LOCUS       DAOSZP010000001        11141 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_4989_length_11141_cov_3.312286, whole genome shotgun sequence.
ACCESSION   DAOSZP010000001 DAOSZP010000000
VERSION     DAOSZP010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 11141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11141
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4989_length_11141_cov_3.312286"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..941)
                     /locus_tag="PK348_00005"
     CDS             complement(<1..941)
                     /locus_tag="PK348_00005"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HXK64626.1"
                     /translation="MTKAKTPHTIEQIMENIQTGDDSIRNKAFEEIIEYGKDAIVPLI
                     TLLAHPDEQIRDSVIWALCELKEHAGQALIQELSNKNPVIRESAAFCLGICKNPHATK
                     ALIPLLKDENEKVRETAAWSLVKIGYDDPQTVQEIVNCLYDDSILVKDTARWILEHIG
                     EPAFEALLQQLLQSSRNIDDIIYILEKIASPQLDRLINLLTTTRDIQQLSYIIQLLGK
                     LREPDAIQYLIPFVNHSNRQIIIITIDALINIGELTIEPLFEKINDETIFPINEMMPL
                     FIRYKKLSVPKLIEMMQSHSLSLRIFAARSLGVIEDKR"
     gene            1223..1399
                     /locus_tag="PK348_00010"
     CDS             1223..1399
                     /locus_tag="PK348_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010632137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HXK64627.1"
                     /translation="MDNTQKVIDAFKKSNEPLNASTIAKMTGLDKKEVDKIMEKLKKD
                     SVIESPKRCYWTLK"
     gene            1457..2212
                     /locus_tag="PK348_00015"
     CDS             1457..2212
                     /locus_tag="PK348_00015"
                     /inference="COORDINATES: protein motif:HMM:NF022112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HXK64628.1"
                     /translation="MATTKKRNSAKTKKSAAKKNISSKKRSSTSSHAPYVLVIMALAA
                     GLIFVLSLKTDTIKQIIPKTNTVVDANKHNSSTNDVSKNSKKDDNHKDFDSKEKLKQE
                     LSHKEKKKEDEVATVQAKVYFLFYNEKADKIELRPVVREVNARVPVKAVLEELIKGPT
                     KVEEKKGYVTAIPPTLKVIDVSIVNNIAFINFNGALEEGAAGNIMLNRLDQIVYTATQ
                     FDNVDGIHILINGQRKRFLGPDGISIAGPLKRH"
     gene            2228..2743
                     /gene="cyaB"
                     /locus_tag="PK348_00020"
     CDS             2228..2743
                     /gene="cyaB"
                     /locus_tag="PK348_00020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IV adenylate cyclase"
                     /protein_id="HXK64629.1"
                     /translation="MLEVEIKSRCSDLDGVKKTIVESGGTFISTEVEEDWYFNHPGRD
                     FGKTDEALRIRKVNDMVFCTYKGPKLGGKTKTRFEKEVQIHDAVAMQEILQKLGFILY
                     GTVQKKRDVYLLNEVTICCDDVKGLGTFVELEMISNNKEEAEKRLFALAEKLGLSDFI
                     TTSYLEMVKGE"
     gene            complement(2750..3157)
                     /locus_tag="PK348_00025"
     CDS             complement(2750..3157)
                     /locus_tag="PK348_00025"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HXK64630.1"
                     /translation="MPEKVFIDTNILIYAFSETEPLKRDTVISLLEFNEIILSTQVIN
                     EFIWVLNKKFKIHFTDIKKLITSLVKNFSIATIDMADIVYAIDIAMKYKFSYWDSLII
                     SSAIKNNCIILYTEDLQNNQVIKNKIKIINPFI"
     gene            complement(3150..3341)
                     /locus_tag="PK348_00030"
     CDS             complement(3150..3341)
                     /locus_tag="PK348_00030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK64631.1"
                     /translation="MIAIKKDYHVKDKYLTIEIPDEFKEQEIEVIIRLKHPVKKEIFL
                     NKIKIDTKKWKFNREEIYA"
     gene            3485..4159
                     /locus_tag="PK348_00035"
     CDS             3485..4159
                     /locus_tag="PK348_00035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK64632.1"
                     /translation="MYKKMMIPCIAIIMLFTGYAIFANEMLKQKIIAFEQSIPWSDVY
                     DGFKTRQHSWHQDVMMAGSPQELGKLLVELQSFIIKEKLGSDWNNIKGAWIAKTGSAT
                     TYDDIAASLKQLQDYYLSSARQDGSDVKKEIITFEQYVPWEDVKDGFKTRQQGWHYDV
                     INAKSPQQAGLLLLEFHDWIYPDKFSDAWTVIKPAWVKKAKNAASWQDVKNLLKELQW
                     NYRKGK"
     gene            complement(4246..5658)
                     /gene="amt"
                     /locus_tag="PK348_00040"
     CDS             complement(4246..5658)
                     /gene="amt"
                     /locus_tag="PK348_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HXK64633.1"
                     /translation="MRLLKISGIIGVVLLCAGLLLAQETNTIVTQVQADSVKMAVVAD
                     TLWVLFAAFLVFFMNLGFAMVEAGLQQAKNAVNISAKNFVVFAISSIAFWFIGWGLMF
                     GDGNGFMGWKGMFMLNGADNSPATGEMYKGVYSSLNWTGVPLYAKFFFELVFCATAAT
                     IVSGAVGGRIKFIAFIVFSFILTSIIYPIAGHWIWGGGWLAKRGFWDFAGSTVVHSIG
                     GWAALTGALVLGPRIGKYNPDGSVNSIPGHSLPLATLGVFVLWFGWFGFNPGSTMAAN
                     AADIGRIAVTTNLAAAAGTLTATITAWLALEKPDLSMILNGALAGLVAITAPCAWVTP
                     GASIVIGGIAGILVVVFVLAFDRWGIDDPVGALSVHLVNGIWGTLAVGLFAAAPYAGG
                     SGQPPLGLFYGGGIGSFANQLVGVVAVGSFVFVLSWVIWIIINATMGIRVTREEELIG
                     LDISEHGIEGYPDFESYTLK"
     gene            complement(5668..7131)
                     /gene="glnA"
                     /locus_tag="PK348_00045"
     CDS             complement(5668..7131)
                     /gene="glnA"
                     /locus_tag="PK348_00045"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HXK64634.1"
                     /translation="MEAIFDPIYLVNGYKEHTSISDILKIIKERNIEFLDLKFTDLFG
                     RLHHYTLTADRADEELFAVGTGFDGSSIRGFQSIHESDMLMRPDPNTAFIDPFFDHRT
                     ISFLCDIIDPVARRRYTKDSRYVGEKAIEYIRSTGIADMAYFGPEPEFFIFDKVQYEQ
                     TQNKAFYEVDSSEASWNSARGEQNLGYKIPYKQGYFPTSPHDQLHNLRSKMVEVLRSC
                     GIKVNLHHHEVATAGQTEIGLVVDELVRQADALVMYKYIIKNVAHRYNKTVTFMPKPI
                     FMDNGSGMHVHVSLWKENRNIFYDFGRYADLSDYARYFIGGIFAHIEAVLAFAAPTTN
                     SYRRLVPGYEAPINLVYSARNRAAEVRIPMYSTSEKANRIEFRCPDPSANPYLAFAAI
                     IMAGMDGIENRIEPPDPIDEDIYELSPHEKRNIRNTPGTLKEVIDGLERDCSFLLKGE
                     VFTVDLIESYIAHKRLHEIDQIALRPHPWEYALYYEI"
     gene            complement(7142..8425)
                     /locus_tag="PK348_00050"
     CDS             complement(7142..8425)
                     /locus_tag="PK348_00050"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HXK64635.1"
                     /translation="MEMYIANIDWALVISIIIIAIAAILIFIKVNLKICEPNEILIFS
                     GRKRKLHSGEVVGYRVIRGGYGLRVPLLERVSRLSLSIIPITLEIEGALSNGMIPLSI
                     VAIAHVKIASKEGGGLENAVERLLGKPSSEVETIAKNTIEGVLRGVIATFTPEEANYQ
                     RIELEKKVYDLAKDELMKLGFTLDSVKIEEIKDSQGYLDAVGRQRNAIVKRDARIKEA
                     EAEAEAKIVEYNARKKASDVEFQSRIAIEEYENNFRERKALLNESTFQKEVRAEYAKK
                     LAELKHLNELEELKKDVNAKKYTAEVIIPAEAEKKAQELKAMGQSSYLKEQGIAMAEA
                     VSKMRAEWQNGNTKELFMLHLLPNIVDSVSSVLKDNLKVEKLVVMGDGGIPKHVGDVT
                     STVVSFIEQIKTATGMDLTKILDEKKSLPVKKELE"
     gene            complement(8447..8788)
                     /locus_tag="PK348_00055"
     CDS             complement(8447..8788)
                     /locus_tag="PK348_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011943147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HXK64636.1"
                     /translation="MKLIIAIIRPEKLADVKKELNEASINLMTVINSIGSGRQKGYPE
                     TYRGVKHEIRLLKKIELQIAVNDDYVEPTIKAITRGAKTGNIGDGKIFVIPIEECVRI
                     RTGERGSRAIG"
     gene            9104..9541
                     /gene="mraZ"
                     /locus_tag="PK348_00060"
     CDS             9104..9541
                     /gene="mraZ"
                     /locus_tag="PK348_00060"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HXK64637.1"
                     /translation="MFMGEYRPTLDEKCRVAIPIKLRKAFGEDNIIHKLIITHGFDKC
                     VMAFREVDWKEFVETKLVPLSQADPMNRMRIRFLLGGATECELDKQGRILIPDYLLEY
                     AQISKDVVMLGLYNRIEIWSAEIYQQYKPDGEALNQFAHDLGF"
     gene            9571..10521
                     /gene="rsmH"
                     /locus_tag="PK348_00065"
     CDS             9571..10521
                     /gene="rsmH"
                     /locus_tag="PK348_00065"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:TIGR00006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HXK64638.1"
                     /translation="MEESTQYYHTPVMPQEVLAFLSGIEGIESSIVVDCTVGEGGHSE
                     LILKTWDGLKLYGFERDREILAIAHKRLSPYGNRVVLINDNFKNLWLHCKGMENRVAA
                     FLYDFGISSFHFDGSNRGFAFKTQEKLDMRLDDSVTLTAYDVVNNFSENELTQIFTTY
                     GQERWSRRIARKIVQKRSEEAIETTEQLANIVLQAIPKQYRVKHIHPATRVFQALRIF
                     INDELSAINESLKHALGYVKPGGRIIAISFHSLEDRIVKERFRRWAKGCCCDNDGLLC
                     QCTANPLVTVLTKKPLQPKESETRQNPRSRSAKLRVCQKV"
     gene            10527..10820
                     /locus_tag="PK348_00070"
     CDS             10527..10820
                     /locus_tag="PK348_00070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK64639.1"
                     /translation="MNTSEQKSIVQYSTIVVILICFIIAFIYQNIEYMQLKMHYNAMI
                     KAYHTILEEYEREKYQLNKALSMEQIEIFAKAHGLEPMTKESLVILKSKQGTH"
     gene            10820..>11141
                     /locus_tag="PK348_00075"
     CDS             10820..>11141
                     /locus_tag="PK348_00075"
                     /inference="COORDINATES: protein motif:HMM:NF013396.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HXK64640.1"
                     /translation="MDIRTYNSFDLYTLLNDTDVTIIKGDEDIRISAIEYDSRKVEKE
                     GLFVAIEGMETDGHKFIKDAVENKASCIVLQENRLDEFGFLLDQQDIAVATAKDTRRA
                     LSQIS"
BASE COUNT         3485 a         2067 c         2183 g         3406 t
ORIGIN      
        1 cgtttatcct caataactcc taaggatcgt gctgcaaaaa tacggaggct gaggctatgc
       61 gattgcatca tttctatgag ttttggcaca gataactttt tgtatcgtat gaacagtggc
      121 atcatctcat tgatggggaa aatggtttca tcatttatct tttcaaaaag tggttcaata
      181 gtcaactcgc caatattaat taaagcatct atggttatga taattatctg tctgttgctg
      241 tgattgacaa acggtatgag gtattgaatg gcatcaggtt cgcgtaactt acctaataat
      301 tggataatgt atgatagctg ctgaatgtca cgtgtggttg ttagaagatt aattaacctg
      361 tcaagttgag gtgaagcaat tttttcaagg atgtatatta tatcatcaat gttgcgggat
      421 gattgcaata attgttgcaa aagtgcttca aacgcaggct caccaatatg ttctaaaatc
      481 caccttgcag tatccttgac caggatactg tcatcgtaca aacaattaac aatttcctgt
      541 acagtttgtg gatcatcata gccaattttt accagtgacc atgctgcggt ttctctaacc
      601 ttttcgtttt catcttttaa aagagggatc aatgcttttg ttgcatgtgg gtttttgcaa
      661 atacctaaac agaaagctgc ggattcacgt ataacgggat ttttgttcga tagttcctgt
      721 ataagggcct gtcctgcatg ttctttcaac tcacataatg cccatattac actgtcgcgt
      781 atctgctcat caggatgtgc aagcaatgta ataagcggaa ctatggcatc tttcccgtat
      841 tctattattt cttcgaatgc tttattgcga atactatcgt cacctgtctg tatgttttcc
      901 attatctgct ctatagtatg tggggtttta gcttttgtca tatagtaata ctgccttatg
      961 tttaagggat tgaattatag taatcaacac ctttgttgaa atatatagtg taaattggat
     1021 attttcaaaa taaaaaatat aattgatttg aatatatgcc atgaataatt aaaaagcaag
     1081 cttattaatt ttacgataca atacatatgc taatagtaag cgtgatatac taccaaagat
     1141 gtgaaatatt ctttacaaaa tgtagtaata atgaaagtat taaattacta aacaccttac
     1201 gttctatttt agagaggtca ctatggataa tacccaaaaa gtaattgatg catttaaaaa
     1261 atcaaatgaa cctttaaacg ccagcacaat tgctaaaatg acagggcttg ataaaaaaga
     1321 agttgataaa ataatggaaa agcttaaaaa ggatagcgta attgaatcgc ctaaacgttg
     1381 ttactggact ttgaaataga ttaaatcata acattaatat acactatact attgatgaaa
     1441 caattatttg aggatcatgg ctaccaccaa aaaaagaaat tctgctaaaa ctaaaaaatc
     1501 agcagcaaag aaaaatattt cttcaaagaa acgttcgtct acatcttcgc atgctcccta
     1561 tgtactggta ataatggcat tggcagctgg tttgattttt gttctgtcat taaaaactga
     1621 taccattaaa cagataattc caaaaaccaa tactgttgtg gatgcaaata aacataatag
     1681 tagcaccaat gatgtaagca agaactcaaa gaaggatgat aatcataaag actttgacag
     1741 caaagaaaaa cttaaacagg aactatcgca caaagaaaaa aagaaagagg atgaagtcgc
     1801 tactgttcag gcaaaggttt attttttatt ctataatgag aaggctgata aaattgaatt
     1861 gcgtcctgtt gtgcgtgaag tgaatgccag ggtccccgta aaagctgtac ttgaagaact
     1921 cataaaaggt ccaacaaaag tagaagagaa gaaaggctat gtgacagcca ttccaccaac
     1981 tcttaaagta attgatgttt cgatagtcaa taatattgca ttcattaatt tcaatggagc
     2041 gcttgaggaa ggtgcagcgg gaaacattat gcttaatcgt cttgatcaga ttgtgtatac
     2101 cgcaacacaa tttgacaatg ttgatggaat acatatttta attaacgggc agcgcaaacg
     2161 tttccttggt cctgatggta tatcgattgc agggccacta aagcgtcatt gaagtatgcg
     2221 aggtataatg ttagaagttg agattaaatc acgatgtagt gatctggatg gtgtaaaaaa
     2281 aacaatagtg gaatcagggg gaacatttat atccactgag gtagaagaag attggtattt
     2341 taatcatcca ggtcgtgatt ttggaaaaac cgatgaggca ttgcggatca gaaaggtcaa
     2401 cgatatggtt ttttgcacct acaaaggccc aaaactgggt ggtaaaacca aaacacggtt
     2461 tgaaaaggaa gtacagattc atgatgccgt agccatgcag gagatattac aaaagttagg
     2521 atttatattg tatggaacag ttcagaaaaa acgcgatgtc tatttgctaa atgaggttac
     2581 catatgctgc gatgatgtta aaggtttggg aacttttgtt gaactggaga tgataagcaa
     2641 taataaggag gaagctgaaa aaaggctctt tgcgctggcc gaaaaattag gactttctga
     2701 ttttattaca acttcatatt tagaaatggt taaaggagag taagccattt tatataaatg
     2761 ggttaatgat tttaattttg tttttaataa cctggttatt ttgtaggtct tcggtgtaaa
     2821 gaattatgca gttattctta attgcagaag aaattattaa actatcccaa tatgaaaatt
     2881 tatatttcat tgctatatct attgcataaa cgatatctgc catatcaata gtagcaatgc
     2941 taaagttttt aactaagctt gtaattaatt tttttatatc agtaaaatgt attttaaatt
     3001 ttttattaag tacccatata aattcattaa taacttgagt gctaagtatt atttcgttaa
     3061 attctaagag tgatatgact gtatctcttt ttaaaggttc agtttctgaa aaggcataaa
     3121 tcagaatgtt tgtatcgata aatactttct caggcataaa tttcttctct gttgaatttc
     3181 catttttttg tatcaatttt tattttgttt aaaaatatct cttttttaac gggatgttta
     3241 agtcttatga ttacttcaat ttcttgttct ttgaattcat caggtatttc tattgttaga
     3301 tatttgtctt ttacatgata atcttttttt attgctatca taatgctacc tctttcaata
     3361 ctgatttcta aacaacaatg tatcagaata attaaaaaag atttgaatta atatatataa
     3421 aataatataa aagtgtaatt tgtcaattag atattttgaa tatttatatt aaggaggatg
     3481 ggctatgtac aaaaaaatga tgataccctg tattgctatt attatgcttt ttactggtta
     3541 tgcaattttt gccaatgaaa tgttgaaaca aaagattatt gcttttgagc aatccatacc
     3601 atggtcagat gtatatgatg gtttcaaaac acgtcagcat tcatggcatc aggatgtcat
     3661 gatggcaggt tcaccacagg aactggggaa gctccttgtt gagttgcaga gtttcattat
     3721 aaaagaaaag ttgggaagtg attggaataa tatcaaaggg gcttggatag ctaaaacagg
     3781 tagtgccact acctatgatg acattgcagc aagtttaaag cagttgcagg attattatct
     3841 tagcagtgca agacaggatg gcagtgatgt taaaaaagaa attattacat ttgaacaata
     3901 tgttccctgg gaagatgtta aagatgggtt taaaacacgt cagcaggggt ggcattatga
     3961 tgttattaat gcaaaatcac ctcagcaggc gggtttattg ttacttgaat ttcatgactg
     4021 gatatacccc gacaagtttt ctgatgcatg gacagttata aaacctgcat gggttaaaaa
     4081 agcaaagaat gctgcaagct ggcaggatgt aaaaaaccta ttaaaagaat tacagtggaa
     4141 ttacaggaaa gggaaataac cttttttatt gatattgatt aattgtataa tcccacgctg
     4201 taaacgacgg cgtgggatta tttgtaatat aactcatata cttttttact ttaaggtata
     4261 tgattcgaaa tcaggatacc cttcaatccc atgttcagag atatcaagcc caataagttc
     4321 ttcttccctt gtaactctta tgcccatggt tgcgttaata ataatccaga taacccagct
     4381 taacacaaag acaaaactgc caactgcaac cacgccaaca agttgattgg caaaagagcc
     4441 tattccacca ccgtaaaaaa ggccaagagg tggttgccca cttccgcctg catacggtgc
     4501 tgcagcaaaa agacccaccg ctaacgttcc ccaaatgcca ttgacaaggt ggactgaaag
     4561 agccccaaca ggatcatcaa taccccatcg gtcaaaggca agaacaaata ccactacaag
     4621 aatgcctgca atcccgccaa tgacaataga tgcaccggga gttacccatg cacagggtgc
     4681 tgtaattgca actaatccag ctaatgctcc attgagtatc atggataaat caggcttttc
     4741 cagtgcaagc catgctgtta tagttgcagt taatgtacca gcagcggctg ccaggttggt
     4801 agttacagca attcgcccta tatccgcagc atttgcagcc attgttgagc ccggattgaa
     4861 cccaaaccag ccaaaccaca gaacaaacac acctaacgtt gcaagtggaa ggctatgccc
     4921 gggtatagaa ttgacactgc catcagggtt gtatttacca atacgaggac ccagtaccag
     4981 tgcacctgtc aatgcagccc agccaccaat ggaatgtacc actgttgaac cggcaaaatc
     5041 ccagaagcca cgctttgcca accagccacc accccatatc cagtggcctg caatggggta
     5101 aatgatactg gtaaggatga atgaaaatac aataaatgca atgaatttta ttcttccgcc
     5161 aactgctccg gaaactatcg ttgctgcagt tgcacagaat accagttcaa agaaaaattt
     5221 ggcatatagt ggtacccctg tccagttaag ggaagagtat acacctttat acatttctcc
     5281 ggttgcgggg ctgttatcag caccatttaa cataaacatt cctttccatc ccataaaacc
     5341 gttgccatcg ccaaacatga gtccccaacc aataaaccag aacgcaattg aagagatagc
     5401 aaacacaaca aagttctttg ccgatatatt aactgcgttt tttgcctgtt gcaagcctgc
     5461 ctcaaccatt gcaaacccta agttcatgaa gaaaactaga aaggctgcaa aaaggaccca
     5521 cagagtatct gcaacaacag ccattttcac tgaatctgcc tgaacctggg taactatagt
     5581 gttggtttcc tgtgccaata gtaagcctgc acacagcaac actacaccaa ttattcctga
     5641 tatttttaat aacctcatag attcctccta aatttcatag taaagtgcat attcccatgg
     5701 atggggtctt aaagctatct ggtctatctc atgcaatcgc ttgtgggcaa tatagctttc
     5761 aatgagatca acagtgaata cttctccttt taaaaggaag gaacaatcac gctcaagacc
     5821 atcaataact tctttcagtg ttccaggggt attacgtatg ttcctctttt catggggcga
     5881 tagttcatag atatcctcat caatggggtc aggtggttca atcctgtttt caatgccatc
     5941 cattccagcc ataataatgg ctgcaaatgc taaatatggg ttagctgaag gatcagggca
     6001 cctgaactca atacgatttg ccttttcgct ggtgctgtac attggtattc taacctcagc
     6061 cgcacggttg cgtgccgaat ataccaggtt gatgggtgcc tcataaccag gaacaagccg
     6121 cctgtatgaa ttggtggtag gggctgcaaa tgccagcact gcctcaatgt gggcaaaaat
     6181 accaccaatg aaataccgtg catagtcact taaatctgca tacctgccaa agtcatagaa
     6241 gatattcctg ttttccttcc acaagctgac atgcacatgc atacctgaac cattatccat
     6301 aaaaatgggc tttggcatga atgtaacggt tttattatat cggtgtgcaa cattcttgat
     6361 aatatacttg tacatcacca gtgcatcagc ctgtctaacc agttcgtcaa caacaagccc
     6421 aatctcagtc tggcctgctg ttgcaacttc atgatgatga agattgactt taataccgca
     6481 tgaacgcagt acttccacca tttttgaacg caggttatga agctggtcat gtggtgatgt
     6541 agggaagtat ccctgcttat agggtatctt ataacctaaa ttttgttcgc ctctggcaga
     6601 gttccagctt gcttcagaag agtcaacttc atagaatgct ttgttctgag tttgttcgta
     6661 ctgtacttta tcaaagatga agaattccgg ttcgggacca aagtatgcca tatcggctat
     6721 accggttgag cggatatatt caattgcttt ttcgcctaca taccgcgaat ctttggtata
     6781 ccgtcttctg gctacaggat caatgatatc acaaaggaat gaaatggtcc tgtggtcaaa
     6841 gaacgggtca ataaacgcag tattagggtc agggcgcatg agcatgtcgc tttcatgaat
     6901 agactgaaat ccccgtattg aggaaccgtc aaaacctgtt cccactgcaa acaactcttc
     6961 atccgctctg tcagcagtta atgtataatg gtgaagcctg ccaaacaggt cagtaaactt
     7021 aagatccaga aattctatat tacgttcttt tattatctta agtatatctg atatggaggt
     7081 atgctcctta tacccattaa ccagatagat agggtcaaaa atagcttcca taaatacctc
     7141 cttattcaag ctcctttttt accggcaaag acttcttttc atctaaaatc tttgttaagt
     7201 ccatccctgt tgcagttttg atttgctcta tgaatgaaac tactgtagaa gtaacatctc
     7261 ctacatgctt gggtatgcca ccatcaccca taacaacaag cttttccact ttcaggttat
     7321 ccttgagaac tgacgatacg ctatccacga tgtttggcaa aaggtgcagc ataaacagtt
     7381 ctttggtatt accattttgc cattcagcac gcatcttgct taccgcttca gccatggcaa
     7441 taccctgttc tttgagatat gatgattgcc ccatggcttt taattcctgt gctttctttt
     7501 cagcttcagc ggggattatt acttcagcag tgtatttttt tgcattaaca tccttcttca
     7561 attcttccag ttcattaaga tgtttcagtt cggccagctt ctttgcatat tctgctcgca
     7621 cttctttctg aaaggttgat tcattcaaca atgccttgcg ttccctgaaa ttgttttcat
     7681 attcttcaat ggcaattctg gactgaaatt caacatcact tgcttttttt ctggcattgt
     7741 actcaacgat ctttgcttcc gcttcggctt cagcttcttt gatacgggca tcacgcttaa
     7801 caatagcatt gcgttgacgc ccaacagcat ctaaatatcc ctgagagtct ttgatttctt
     7861 caattttaac tgagtctaag gtaaatccca gtttcattaa ttcatctttg gctaaatcat
     7921 atactttctt ttcaagttct atcctctggt agttggcttc ctctggcgtg aatgttgcaa
     7981 taactccacg cagaacacct tcgatggtat tcttagcaat agtttcaacc tcggatgaag
     8041 gtttgccaag gagcctttca acagcatttt ccagtccacc accttctttt gaagcaatct
     8101 taacatgagc tatcgccaca atacttaatg ggatcatacc gttggaaagc gccccttcta
     8161 tctccagagt gatggggata attgaaagtg acaaccgtga aacgcgctct agaagaggaa
     8221 cacgcagacc ataacctcca cgtattacac gatatcccac aacttctccg ctgtggagtt
     8281 tgcgtttacg ccccgagaaa atcagaatct cgtttggctc gcaaatttta agattgactt
     8341 tgataaaaat aagaattgct gcaattgcaa tgatgattat tgaaataacc agtgcccaat
     8401 caatatttgc tatgtacatt tccattgttt gcctcctttt aagtaattaa cctatggctc
     8461 ttgatccgcg ttcgcctgtg cggatacgaa cacattcctc aattggtatg acaaaaattt
     8521 tgccatcgcc aatattacca gtctttgctc cacgagttat tgcttttata gttggctcaa
     8581 cgtaatcgtc attgacagca atctgaagct caattttttt caaaaggcgt atctcatgtt
     8641 ttacaccacg gtaggtttcg gggtatcctt tctgcctgcc tgagccaatt gagttgatta
     8701 ccgtcattaa attaatactg gcttcattta actccttttt tacatccgct aatttttcgg
     8761 gacggatgat tgcgataatt aatttcataa aacctcctta ctatttcatt aaattatgta
     8821 gttgtggatt gtgttatcat tctcacccaa ctacattcca gtactggttc tatgtataga
     8881 gtagcagtga ggtattatag gggtcaatgt ttggttaaaa tgaggcgatt attaaccttt
     8941 tatttaacat atgtttaatc attttgtaac ataattgaaa caattggtag atatataaat
     9001 tttcattttt ttcttgacaa ttatgtcaca ttgtgtctat aagtggtaaa aagtggggat
     9061 tagtgcagaa tagtagaaat gtaaggggaa agtatagtat actatgttta tgggtgaata
     9121 ccgccctacg cttgacgaaa aatgccgagt tgcaataccc attaaactgc gtaaagcgtt
     9181 tggcgaagac aacatcatac acaaattaat cataacgcat ggttttgata aatgcgttat
     9241 ggctttcagg gaagttgact ggaaggaatt tgtggaaaca aaactggtac ctctttcaca
     9301 ggctgacccc atgaacagaa tgcgtatcag gtttttattg ggtggtgcaa cggaatgtga
     9361 gctggataaa cagggaagga ttcttatccc tgattatctg cttgagtatg cacagatcag
     9421 taaggatgtt gtcatgttgg gtttgtataa ccgcatcgaa atatggagtg cagaaattta
     9481 tcagcaatac aaacctgatg gagaagcgtt gaatcagttt gcacatgatc tgggatttta
     9541 acaatgaact atagtgtaat gtgacataat atggaagaaa gtacacaata ctatcacacc
     9601 cccgtaatgc ctcaggaagt gcttgctttt ctttctggca ttgagggaat agaatcatct
     9661 attgtggtag attgtacagt tggtgaaggt gggcattcgg aattaatcct taaaacatgg
     9721 gatggattga agctgtatgg atttgaacgg gacagggaaa ttttggcgat agctcataaa
     9781 aggctgtcac catatgggaa tagagtggtg ttaattaatg acaattttaa aaatctctgg
     9841 ctccattgca aggggatgga aaatagagta gctgcgtttt tatatgactt tggaatttct
     9901 tcgttccatt ttgatggcag caatcggggg tttgcattca aaacacagga aaagttagat
     9961 atgcgccttg acgattcagt gacgcttact gcatatgatg tggtgaataa tttcagtgaa
    10021 aatgaattga cacaaatttt taccacatat ggccaggaac gctggtcgcg tcgtattgcc
    10081 cgtaagattg tccagaagcg aagtgaagaa gctattgaaa ccacagagca gttggcaaac
    10141 attgtgctgc aggcaattcc aaaacagtac agggttaaac atattcatcc tgctacacgg
    10201 gtttttcagg ctttacggat ttttattaat gatgaactgt cggcaattaa tgaatcactg
    10261 aaacatgcac tgggatatgt aaaaccaggc ggaagaatta ttgccatatc atttcattca
    10321 cttgaggatc gcattgtaaa ggaaagattc agacgatggg ccaaagggtg ttgctgcgat
    10381 aatgacgggt tgttgtgtca gtgcacagct aatcctcttg ttactgtgtt gacaaaaaag
    10441 ccactgcagc ctaaagagtc cgaaaccaga cagaatccac gttcgcgcag tgcaaaatta
    10501 cgggtatgcc agaaggtatg atgagcatga atacatcaga acaaaaaagt atagtgcaat
    10561 acagtaccat tgttgtcatt cttatatgtt tcatcattgc attcatttat cagaatattg
    10621 aatatatgca gcttaagatg cactataatg ctatgataaa agcgtatcat acaatacttg
    10681 aagaatatga acgtgaaaag tatcagctga ataaagcact gtcaatggag cagatagaga
    10741 tatttgcaaa agctcatggt ctggagccca tgactaaaga aagcctggta atactgaaaa
    10801 gcaaacaagg tacacattaa tggatatacg aacatataac tcgtttgact tatatacatt
    10861 acttaatgat acagatgtca caattataaa aggtgatgag gatatacgta tcagcgccat
    10921 tgaatatgac tcgcggaagg ttgaaaaaga gggactgttt gttgctattg agggaatgga
    10981 aaccgacggc cataaattca taaaagatgc tgttgaaaac aaagcatcat gcatagtatt
    11041 gcaggaaaat cgcctggatg aatttggctt tttacttgat caacaggata ttgcagttgc
    11101 aacagcaaag gatacaagaa gggcactgtc acagatttca g
//
LOCUS       DAOSZP010000002        10480 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_5402_length_10480_cov_3.430312, whole genome shotgun sequence.
ACCESSION   DAOSZP010000002 DAOSZP010000000
VERSION     DAOSZP010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 10480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10480
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5402_length_10480_cov_3.430312"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="PK348_00080"
     CDS             <1..339
                     /locus_tag="PK348_00080"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HXK64641.1"
                     /translation="YSGKLLAFLLLTDAFKALSLSALIVILFFPYTFTGLFGLSFSIG
                     SYSITPLIEIIFFIGKVVIVYSIGVTVIRVIMARLKISQVAKVFLFAVSAISIIGMVL
                     IMLDPKMKMI"
     gene            350..817
                     /locus_tag="PK348_00085"
     CDS             350..817
                     /locus_tag="PK348_00085"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HXK64642.1"
                     /translation="MLGMVYKVVEQLFAKKSTNYFPSKYILEDTADTLKKGTIHPPVA
                     VKEGFRGRLEYNVESCTGCGLCSKVCPANAIELYPAVINDKKTKRIVIYLSRCTYCQE
                     CVNICPKNSIQITTHFFMADYNKYDASLVVGLEHRKKYEVKEEGSPDVQKEGN"
     gene            795..1058
                     /locus_tag="PK348_00090"
     CDS             795..1058
                     /locus_tag="PK348_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK64643.1"
                     /translation="MFKKKEIEIKVKLYSGLHKDASIDRYNPDEGYILKIPKGSRVRY
                     VVKQLGLPDIYSLAYFCNGQRIGIFTKLREGDELSCFRPSGGG"
     gene            complement(1060..1785)
                     /locus_tag="PK348_00095"
     CDS             complement(1060..1785)
                     /locus_tag="PK348_00095"
                     /inference="COORDINATES: protein motif:HMM:NF013348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine biosynthesis TtcA family
                     protein"
                     /protein_id="HXK64644.1"
                     /translation="MSTLTNKHITKLLKVVGKAINTYSMISSNDNVLIALSGGKDSLV
                     LMDALHRRLQHIPITYNLYCCHVSVTNFHNKETIETLRTYCQHYSIPLYVKEITLDLS
                     DTNASPCFVCSWFRRKALFETAAQLQCKKIAFGHNQDDIIVTFFMNMLYHGKLSTMPP
                     KLKMFNGALEIIRPLAFLTEAEVKGYCNALPLVPLENDCPYKDTTRRKQTKELLKIIN
                     KSVKGAQSSIFRSLRHRDDEYLL"
     gene            1894..2250
                     /locus_tag="PK348_00100"
     CDS             1894..2250
                     /locus_tag="PK348_00100"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXK64645.1"
                     /translation="MARCMIVDDSSFMRRIIRQTLQEGGHEVVAEVDNGLEALEKYRE
                     IQPDVVTMDITMWGKDGFEAISEITDSYPDAKIIVISALSERTLKINEKDIKARVFLT
                     KPFEKKELLEAINKVL"
     gene            2252..3298
                     /locus_tag="PK348_00105"
     CDS             2252..3298
                     /locus_tag="PK348_00105"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HXK64646.1"
                     /translation="MEEQLLQLYKKSTVADSAYLIADGKAYLFVTEADKYLLQGKNLI
                     IGATELVLGAKTNENILRLETALALKGTTIKKIPAATLIQGLDNSAFAMNLAIVEAKM
                     LVLTNEILKKDLQLDSQLVKEKNICIEYFTIIQSLQKEYDKRRLPWIKELIEKYKTSL
                     IYKKGEAFTRATESLKVETPRELSSNTIEFPPGSVLCQEGSIGDFMYILESGQLEVYI
                     QNAKVATIAEKGTVIGEIAMLLSMPRTATLKAQNTVLVTKVTRDDLKQSSSPQLIQML
                     LVSLAKKHQANVMHIEDINEKKALAYAEKKEQEPHKTDIHRYINELSQLKSGLEKLIN
                     TKQADYLEYILKIK"
     gene            complement(3302..3835)
                     /locus_tag="PK348_00110"
     CDS             complement(3302..3835)
                     /locus_tag="PK348_00110"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HXK64647.1"
                     /translation="MKIEITILGLLMEENLYGYEIKKRIVERLEDYVDIKFGSIYYAI
                     KKALDNGWVKKIGTEKEGGNPERYIYQILPDGRKYYKKMLKQYFDHNFIHFDIDIVLM
                     FLNSLSPEQREQFIEDRLEHIKEKMNALREKIEAESKNTSPASQLHVYTYLENHLKAE
                     LNWLRSLKKGEVLTEVE"
     gene            complement(4031..4618)
                     /gene="ruvA"
                     /locus_tag="PK348_00115"
     CDS             complement(4031..4618)
                     /gene="ruvA"
                     /locus_tag="PK348_00115"
                     /inference="COORDINATES: protein motif:HMM:NF001040.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HXK64648.1"
                     /translation="MISFLKGNILSCKPTRVVLDVQGVGYEIHIPLSTYEKISTARST
                     ELFIHMVVRENAILLYGFNSEEEQQIFSLLLTISGIGPSIAISILSSMTPQQILSAVK
                     NDQITAFTSIPGIGQSKSEKIIFELKRKLKKLDTILSRESTTTPYMKDAIEALVSLGF
                     DEKQASSTVSSVIAQHSDINNIETIIRLSLKQLSK"
     gene            complement(4646..5131)
                     /gene="ruvC"
                     /locus_tag="PK348_00120"
     CDS             complement(4646..5131)
                     /gene="ruvC"
                     /locus_tag="PK348_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008537374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HXK64649.1"
                     /translation="MAYLLGIDPGYGRCGWAIISDSFSYIDCGVIETKPTDENRLLDV
                     YTSLSDIIQKYSPVSCGVEKLFFNKNVKTAIPVAQVIGVIKLLLAQHNIPYAEYTPVQ
                     IKQSITGYGRSSKDQLAFMVKKILKTPEIIQYDDAFDALAIALCHCLALPGYSIIKKN
                     I"
     gene            complement(5138..5884)
                     /locus_tag="PK348_00125"
     CDS             complement(5138..5884)
                     /locus_tag="PK348_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013163435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HXK64650.1"
                     /translation="MSGHSKWANIKHRKAAQDAKKGKVFTKLIREITVAARVGGDDLN
                     SNPRLRTAVLKARENNMPMDNIERAIKKGTGALEGSTYEEVRYEGYGPGGVAIMVECM
                     TDNKNRTTPEIRTIFSKNGGNLGETGCVAYLFDKKGVINIDANATTEDNVMEILLDYD
                     IEDIKTEDNTIVVYTAPEKFNDILEVIRSNNLPTLNSEITYVPQTTVHLEGQKASQCL
                     RLIEALEDHDDVQSVHANFDIPDDIINNMS"
     gene            complement(6006..7286)
                     /locus_tag="PK348_00130"
     CDS             complement(6006..7286)
                     /locus_tag="PK348_00130"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK64651.1"
                     /translation="MKFKSLILYLSIFSILLLIDNGSGQDYVALIQQNKFTDALQIIQ
                     LQLDEMYSSRSIDKKVPDSYVAIGKIEEGIDLKKLFTDRKLQPYFIENNDTLYTLHIN
                     AALCYLNMFKYKEAVQHYFQALRFTTISEKDHTIFYSLALLFKRLNKYDAYLTYLEEA
                     YEIKPDNYDYSLELALALASGKNKKKALFHLNRYIQAKGNDTPPELYLTAANCYESIG
                     DFINAGRYYQLYLTIHPDDAAIQFALGYLAYTKISDMKLAYTSLSKGLSLYGDSDLIR
                     KGISHAIIGDITSMDLNYTESLNHYLQAIQISERIHQSIEDKKNSIEGIKIKINTIKS
                     TLLDKKDISLYPEYQSLMDELGNNELELRRLEHEYSKLTVGELYFKIASTYENTMQYQ
                     QAIDWYTRAIASGTKIRESSKKIEKIRLKISRGY"
     gene            complement(7352..7966)
                     /gene="pth"
                     /locus_tag="PK348_00135"
     CDS             complement(7352..7966)
                     /gene="pth"
                     /locus_tag="PK348_00135"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010573531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HXK64652.1"
                     /translation="MKLIVGFGNPGEEYFNNRQNIGFKVVDILGNNENIDIRIKKKKS
                     IIGRGKISGEDVVLLKPQTFVTLLGESVLYIASFLRINVRDIICVLEDPSLPLGEIRI
                     DSIMSKLKHPGIESITRALKSDRFAKVRIGIGYPKKGVTMEEHLLNDFSEDENFILID
                     VLNKAEEVVRMLITHSIEDVQNKYNPEGAPKIKKRQLPKIRIRR"
     gene            complement(7991..8614)
                     /locus_tag="PK348_00140"
     CDS             complement(7991..8614)
                     /locus_tag="PK348_00140"
                     /inference="COORDINATES: protein motif:HMM:NF004139.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HXK64653.1"
                     /translation="MEAHVLPVETRTQIGKNANHKLRAQGYIPAVLYSHGESQTIMVQ
                     KKNFFKIFKGHISENVLIDLDIKDSKGSPVKAFVKDYQRHPITDEILHVDFFRVTMTE
                     AISTKVPVEISGTSIGVKQGGILEIIEREIEVECLPADLPEKITIDVTNLSIGQSIHV
                     KDIAAPKGVTILSTPETVIVAVLAPHKAAEEVAQPVAEEVQPAEEQQ"
     gene            complement(8688..9635)
                     /locus_tag="PK348_00145"
     CDS             complement(8688..9635)
                     /locus_tag="PK348_00145"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HXK64654.1"
                     /translation="MIYPIKLFSGTSNFPLAQEIAQYLGIELSEIEIKKFKDGEISVK
                     ILENVRSVDVFLIQSTSNPANDNIMELLLMVDAAMRASAQRITAVIPYYGYARQDRKV
                     EPRVPISAKLVANMLQAAGVDRILTMELHSDQIQGFFDIPVDNLFASPLVIEYVRELN
                     ILNPTVVSPDAGGTERARFLARNIGAELAIVDKRRPEPNKSEVMNIIGEESVKNHNCI
                     LIDDMIDTAGSITQAAHALRENGAKDIYCIATHAVLSENATEKLKKADFKEIILTNTI
                     DISEKKLLPNMKILSMAPLFGEAIRRIHNGESVSSLFVR"
     gene            complement(9651..>10480)
                     /locus_tag="PK348_00150"
     CDS             complement(9651..>10480)
                     /locus_tag="PK348_00150"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HXK64655.1"
                     /translation="TAPGFGPGIPRFESWHLRFFILLVPCMDSNCKAIVLAAGKGVRM
                     QSEMPKVLHHLNGKPLVLHVIDNLIHAGLKEEDIIVVVGYRGEDVIQAIGNRARFVWQ
                     KEQLGTGHAVMQAEPVLQAYNGIVIVACGDVPLIKPETFSMLFSTANDPKTGAVVLTM
                     ELENPFGYGRIIRDDNGNLQRIVEEKDAYPVQKAIHEVNTGTYAFKSQLLFKGLKTVT
                     TENAQREYYLPDALTYIRSTGNTVKIIKLHDPIEGSGINSKEELEKLEDIISKKSNN"
BASE COUNT         3215 a         1818 c         1898 g         3549 t
ORIGIN      
        1 tattcgggga agttattagc atttttgcta ttaactgatg cgtttaaagc attgtcatta
       61 tcggctttga tagttatatt atttttcccc tatacattta caggtttgtt tgggttatct
      121 ttttccattg gtagttatag tattactcca ctgattgaaa taatcttttt tataggaaaa
      181 gttgtcattg tatatagtat tggagtaact gtaataaggg taattatggc acggttgaag
      241 atttctcagg tagccaaggt gtttctattt gcagtttctg ctataagtat tattggaatg
      301 gtgctcatta tgttagatcc aaagatgaaa atgatatgaa aggtgagcca tgttaggaat
      361 ggtatataaa gtggtagagc agttgtttgc aaaaaaatct acaaattatt tcccttcaaa
      421 atatatactg gaggatacag cagatactct taaaaaaggt acaatacatc cacccgttgc
      481 ggtgaaagaa ggtttcagag gcagattaga atataatgtt gaaagttgta ccggttgcgg
      541 attatgcagc aaggtatgcc ctgccaatgc tattgaatta tatcctgctg tcattaatga
      601 taaaaaaaca aagcgaattg tgatatattt atcccggtgc acctactgtc aggaatgtgt
      661 taatatatgt cccaaaaatt caatacagat tactacacat ttttttatgg ctgattacaa
      721 taaatatgat gcttcactgg ttgttggttt ggaacacaga aagaaatatg aagtaaaaga
      781 agaaggatca ccggatgttc aaaaagaagg aaattgaaat aaaggttaaa ttgtatagcg
      841 gattacataa agatgccagc attgacagat ataacccgga tgaagggtat atactgaaaa
      901 ttcctaaggg ttcccgcgtt agatatgtgg taaaacaatt aggattgccg gatatctatt
      961 cgttggcata tttttgtaat ggacagcgaa ttgggatttt tacaaagctt cgtgaaggtg
     1021 atgaactttc ttgcttcagg ccttccggcg gaggttgatt tacaacagat attcatcatc
     1081 cctgtgacgt aatgatctaa aaatgcttga ttgtgcacct tttaccgatt tattaataat
     1141 ttttaatagt tcttttgtct gctttcgcct ggttgtatct ttataggggc agtcattttc
     1201 caaaggtaca agcggtaatg cattgcaata acctttcact tctgcttcag tgagaaatgc
     1261 aagtggcctg attatctcta atgcgccatt gaacattttt aattttggcg gcattgtcga
     1321 tagtttccca tgatagagca tattcataaa aaatgtgacg ataatatcat cctgattgtg
     1381 accaaaggca atttttttgc actgaagctg ggcagcagtt tcaaataagg cttttcttct
     1441 gaaccatgaa cagacaaaac atggtgatgc attggtatca gaaagatcta atgttatttc
     1501 ttttacgtat aaaggtatgc tgtaatgctg gcagtatgtc ctgagtgttt caatagtttc
     1561 tttgttatga aagtttgtaa cagagacatg gcagcaatac aaattatatg taatgggaat
     1621 gtgttgtaaa cgacgatgta atgcatccat caaaacaaga gaatctttcc cgcccgaaag
     1681 agcaatgagt acattatcat tagaactaat catgctgtag gtatttatgg ctttaccaac
     1741 aacttttaaa agctttgtaa tgtgtttgtt tgtgagagta ctcataataa agccttgacg
     1801 taaagattat ggtatgatta agtagtgctt ggaaaggagt agttgtcaat agaatttgta
     1861 cgtaattatc atagttaatg cagaggtttt gtaatggctc gttgtatgat agttgatgat
     1921 tcaagtttta tgcgccgtat tatcagacaa acattgcagg aaggtggtca tgaggtagtg
     1981 gcagaggttg ataatggcct tgaagccctg gaaaaatacc gagaaataca gcccgacgta
     2041 gtcaccatgg atattaccat gtggggcaaa gatggctttg aagcaatatc tgaaataacc
     2101 gatagttacc cggatgctaa aattattgtg atatcagcat taagtgaaag aacattgaaa
     2161 ataaatgaga aggatattaa agcccgtgtg tttttaacca agccgtttga aaagaaagag
     2221 ctgttagagg caatcaataa agtcctgtaa catggaagag caattattac aattatataa
     2281 aaaaagtact gtggcagata gcgcatattt aattgccgac ggcaaggcat atttatttgt
     2341 tacagaagct gataagtatc ttttacaggg taaaaatctt attattggtg ccactgaact
     2401 ggtattgggt gcaaaaacta atgaaaatat attgaggctt gaaacagcat tagcgcttaa
     2461 agggacaaca ataaaaaaaa ttcctgctgc aacacttata cagggattgg acaatagtgc
     2521 gtttgctatg aatctggcaa ttgttgaagc caaaatgttg gtcctaacca atgaaatctt
     2581 gaaaaaagat ttgcaactgg attcacagct tgtaaaagaa aaaaacatat gtattgaata
     2641 ttttacaata attcaatctc tccaaaaaga atatgataaa agaaggcttc catggatcaa
     2701 ggaacttatt gaaaaatata aaaccagttt gatatataaa aaaggcgaag catttacgcg
     2761 ggcaacagaa tcactaaaag ttgaaacccc ccgggagctt tcttcaaaca ctatagaatt
     2821 tcctcctggt tctgttttat gtcaggaagg cagcattggt gattttatgt atatactgga
     2881 atcaggtcag ctcgaggtgt atatacagaa tgcaaaagtt gccacaatag ctgaaaaagg
     2941 aacagtcatt ggggagatag caatgttact atctatgccg cgaactgcca cactaaaggc
     3001 ccagaataca gttctggtta caaaagtaac cagggatgat ttgaaacaat cttcctcacc
     3061 tcagcttata cagatgttac tggtttcact tgccaagaaa catcaggcaa atgttatgca
     3121 tattgaagat attaatgaga aaaaagctct tgcctatgca gaaaagaaag agcaagagcc
     3181 tcataaaact gatattcacc gttatattaa tgaattatcg cagcttaaaa gtggattaga
     3241 aaaactaatc aacactaaac aggctgatta tttagaatat atactgaaaa ttaaataaaa
     3301 attattcaac ctcagttaat acttcacctt tctttaatga tcgcagccag ttgagctcag
     3361 cttttaaatg attttccaga tatgtgtaca catgtaactg tgatgcgggt gatgtattct
     3421 tagattcagc ttcaatcttt tctcgcaatg cattcatttt ttctttgata tgttccagac
     3481 gatcttcaat gaactgttcg cgctgttctg gtgaaagaga atttaaaaac atcaacacta
     3541 tatcaatatc aaaatgtata aaattgtggt caaagtactg ttttaacatc ttcttgtaat
     3601 actttcttcc atccggtaat atctggtata tataccgctc aggatttccg ccttcttttt
     3661 ccgttccaat ttttttaacc cagccattat ccagggcttt ttttattgca taataaatag
     3721 acccaaattt gatatcaaca taatcttcaa ggcgttcaac tatccttttt ttgatttcat
     3781 agccataaag attttcctcc ataaggagcc ccaatatggt aatttcgatt ttcatattta
     3841 cctctctata aaaattaaat tatttctttt aaaatacaat gaagattgaa aaaaagcaat
     3901 aataatattg tatgtttata aaaatatttt aaaaataatt taaattagat tatataaata
     3961 tgactttata tatagttaca tttaatatag tttaatttta ctatttttat tatatcacaa
     4021 atattctgga ttatttagat agttgtttta atgacaaacg gataattgtt tcaatattgt
     4081 ttatgtcact atgttgtgca atgaccgatg aaacagtaga tgaagcctgc ttttcatcaa
     4141 atcctaacga taccaatgct tcaatagcat ctttcatata tggtgttgta gtagattccc
     4201 gggacaggat tgtatctagt ttttttaatt ttctttttaa ttcaaaaata attttttcac
     4261 ttttgctctg gcctatgccc ggtatggatg taaatgctgt tatttggtca ttttttacag
     4321 ccgatagtat ctgctgagga gtcatggatg ataaaattga aattgctatt gacgggccta
     4381 ttccagaaat ggtcagcaga agtgaaaata tctgttgctc ttcttcggag ttaaatccat
     4441 acaacaatat ggcattttcc cttacaacca tatgaataaa aagctctgta cttctagccg
     4501 tactaatttt ttcataggtt gacaggggaa tatgaatttc atagcccaca ccctgaacat
     4561 caagaacaac cctggtaggt ttacaagaaa gtatatttcc ctttaaaaac gatatcatag
     4621 tatgttataa taaacccttt tactattaaa tatttttttt gattattgag taacctggta
     4681 aagctaaaca gtgacataat gcaatggcaa gtgcgtcaaa ggcatcatca tactgtatga
     4741 tttctggagt ttttaatatt ttttttacca tgaatgcaag ctgatccttt gaagaacgcc
     4801 cgtaccccgt tattgattgc tttatctgta caggtgtata ttcagcatac ggtatattgt
     4861 gctgagccag caacaatttt atcacaccaa taacctgtgc aactggaatt gctgttttaa
     4921 catttttatt gaaaaacaat ttttcaactc cacaggatac cgggctatat ttttgtatga
     4981 tatctgaaag tgaagtatat acatctaaca aacgattttc atcagtgggc tttgtttcga
     5041 taactccaca atcgatatat gaaaaagaat cacttatgat agcccagccg caacgcccat
     5101 atcccggatc aataccaagt aaataagcca cgtaatatca actcatattg ttaataatat
     5161 catcagggat atcaaaatta gcatgaacac tctgaacatc atcatgatct tccagtgctt
     5221 caatgagtct aagacattgc gaagcttttt gcccttctag atgtactgtt gtctggggaa
     5281 cataggtaat ttcgctattc agagttggta aattatttga acgaataact tctaaaatat
     5341 cattaaactt ttcaggggca gtgtaaacaa caattgtatt gtcttctgtc ttaatatcct
     5401 caatatcata atcaagtaaa atttccatga cgttgtcttc ggttgttgcg tttgcatcaa
     5461 tgtttataac gcctttttta tcaaaaagat atgcaacgca tcctgtttca cccagatttc
     5521 cgccattttt agaaaaaata gtgcgtattt caggagtagt tcggttttta ttatcggtca
     5581 tacactcaac cattatagcc actccaccag gtccatatcc ttcataccga acttcttcat
     5641 atgttgagcc ttcaagggcg ccagtacctt ttttaattgc tctttcaatg ttatccattg
     5701 gcatattatt ttcacgagct tttaatacag cggtacgtaa tctaggatta ctgttcagat
     5761 catcgccacc aacccgtgct gcaacagtta tttcgcgtat gagcttggta aataccttac
     5821 cttttttggc atcctgtgcc gctttcctgt gttttatgtt tgcccattta gaatgtcctg
     5881 acatgtttta ttctcctcaa tgtacaagtt ttctagtgtt attacaaagc aagaacgaat
     5941 ggtagaatca tgactaattt tgtcattaat aaaataatca caaaatttgt gtaaaaataa
     6001 ttttttcaat aaccacgtga aattttcaac cgtatttttt caattttttt tgaggattca
     6061 cggatttttg tacctgaagc gatagctctg gtataccagt ctattgcctg ctgatattgc
     6121 atggtatttt catacgttga agcaattttg aagtacagtt cccctacggt aagtttgcta
     6181 tattcatgct caaggcggcg taattctaat tcattatttc ctaattcatc cataagagac
     6241 tggtattcgg ggtagagtga tatgtccttt ttatcaagta gtgtagattt aatggtgtta
     6301 attttgattt tgatcccttc aatactattt tttttatctt ctatggactg atgtattctt
     6361 tcagaaattt gtatagcctg gagatagtgg ttgagacttt ctgtgtaatt taaatccata
     6421 ctggttatat caccaattat tgcatgtgat attcctttcc ttatcagatc actatcgcca
     6481 tagagtgaca gcccttttga tagtgaggta taggccaatt tcatgtctga aattttagta
     6541 taggctaaat acccaagagc aaattgtata gctgcatcat cagggtggat agtaagatat
     6601 aattgataat agcgtcctgc gtttataaag tcacctatag attcatagca gtttgcagca
     6661 gttaaataaa gttcaggagg ggtatcgttg ccttttgcct gtatataacg gttgagatga
     6721 aaaagtgctt ttttcttgtt tttaccagaa gcaagcgcca gagctaattc taatgagtaa
     6781 tcataattat caggtttaat ttcatatgct tcttccaaat aagtcagata tgcgtcatat
     6841 ttattgaggc gtttaaaaag gagagcaagg gaatagaaga ttgtatggtc tttttcactt
     6901 atggtggtaa atcgtaatgc ctggaagtaa tgttgtactg cttctttgta tttgaacata
     6961 ttaagatagc aaagggcagc attgatatgt agtgtataaa gtgtatcatt attttctata
     7021 aaatatggct gtaattttct atcagtaaat aattttttca agtctatgcc ctcttctatt
     7081 tttccaattg caacatatga gtcaggaacc tttttatcaa tacttctgga agaatacatt
     7141 tcatctaatt gcaattgtat tatctgcagg gcatcggtga atttattttg ctgtatcaga
     7201 gcaacataat cctgcccact gccattatca atgagcagca atatggaaaa tatagaaagg
     7261 tacaaaatta gcgatttaaa tttcataata gttgttttga ctgtttagta gtattatcgg
     7321 atggtttgaa aagcaagttg aattacagga attatcgccg aatacgtatt ttcggaagtt
     7381 gacgtttctt gatttttggg gcgccttctg gattatattt attctgaaca tcttcaatgc
     7441 tatgagttat tagcattctg accacttctt cagctttgtt aagtacatct attagtataa
     7501 aattttcatc ttcagaaaaa tcattgagaa gatgttcttc catggtcacg ccttttttag
     7561 gatagccaat acctattcta accttagcaa acctgtcaga ttttaacgcg cgggtaatgg
     7621 attcaattcc cgggtgtttt agctttgaca tgatggagtc tattcgtatc tcgccaagtg
     7681 gcagggatgg gtcttccagc acacatatga tatcacgtac attaattcgt agaaaagaag
     7741 caatgtaaag aacagactca cctaaaaggg taacaaaggt ttgaggtttt agcaaaacaa
     7801 catcttcgcc agaaatcttg ccacggccta taattgattt tttcttttta atgcggatgt
     7861 cgatattctc gttattacca agaatatcga caaccttaaa tccaatgttt tgtctgttgt
     7921 taaaatattc ttcgcctgga tttccaaatc ctacgattag cttcaattat taactcctta
     7981 tattaaattg ttattgctgt tcttcagcag gctgaacttc ttctgcaact ggctgtgcaa
     8041 cttcttcagc tgccttatga ggtgcaagaa cagctacaat tactgtctca ggggtactaa
     8101 gaattgtaac acctttggga gcagcaatat cctttacatg tatagattgc ccaattgaaa
     8161 gatttgttac atcgatagta attttttctg gtaaatctgc aggaagacat tcaacctcaa
     8221 tttcacgctc aataatttca agtatgccgc cctgtttaac accaatagaa gttccgctaa
     8281 tttccacagg tacttttgta gaaatagctt cggtcatggt tactctaaaa aaatctacat
     8341 gcagtatttc gtctgttatg ggatgtcgct ggtaatcctt gacaaaggct ttaactggag
     8401 agcctttaga atctttaatg tcaaggtcta taaggacatt ttcggatata tggcctttaa
     8461 aaattttaaa gaagtttttc ttctgaacca ttatggtctg agattcacca tgagagtaca
     8521 atactgcagg tatatacccc tgtgctctga gcttatgatt tgcatttttc cctatctggg
     8581 ttcttgtttc aactggtaaa acgtgtgctt ccatatcaac ctcttttaaa ttatttaata
     8641 tacattatag tgaaaatgtt ataaaattca ataataaatt ttttcaacta tcggacaaac
     8701 aatgaactaa ctgattcgcc gttatgtatg cgacgtatag cttcaccaaa cagtggagcc
     8761 attgataata ttttcatgtt tggcagaagt ttcttttctg atatatcaat tgtattcgtc
     8821 aaaatgattt ctttaaaatc tgctttttta agcttttcag tagcattttc agataatact
     8881 gcatgcgttg caatacagta tatatccttt gcgccatttt cccgtaatgc gtgtgctgcc
     8941 tgtgttatgg aacctgctgt atcaatcata tcgtcaatta atatacagtt gtggtttttt
     9001 acactttctt cgccaatgat attcattacc tcggatttat tgggttcagg ccttcgctta
     9061 tctactatcg ctaattctgc accaatattc cttgctaaaa accgtgcacg ttcagttcca
     9121 ccagcatccg gtgatactac ggtgggattt agtatattga gttctcgtac atattcaatc
     9181 accagtggcg atgcaaaaag attatccacc ggaatgtcaa aaaagccctg tatctgatca
     9241 gagtgtaact ccatggtaag aattcggtcc accccagcag cctgcaacat atttgcaact
     9301 aattttgctg aaatgggaac ccggggttca acctttcgat cctggcgagc atagccgtag
     9361 taagggataa cagcagtaat tcgctgtgct gaagctcgca tggcagcatc caccataagc
     9421 agcaattcca tgatgttatc gtttgcaggg ttactggtgg attgtattaa aaaaacatca
     9481 acgcttcgca cgttttcaag aatcttgaca gatatttccc cgtccttaaa ttttttaatt
     9541 tcaatttctg aaagttcaat gcctaaatat tgtgctattt cttgggctaa cgggaaattt
     9601 gaagttccgg aaaatagttt tattggatat atcatataaa accctttttg ttaattgtta
     9661 ctttttttag aaataatatc ttcaagtttt tctaattcct cttttgaatt tataccactg
     9721 ccttctattg gatcatgtaa tttaataatt ttgacagtat taccggttga ccggatatat
     9781 gttaatgcat caggaagata gtattctctc tgtgcatttt cagtagtaac agtttttaaa
     9841 cccttgaaaa gaagctgtga tttaaatgca taggttcctg tattaacttc atggattgct
     9901 ttttgcacag ggtatgcatc cttttcttcc acgattcttt gcaggttacc gttatcatcg
     9961 cgtattatcc gcccatagcc aaagggattt tctagttcca tggtaagcac aactgctccg
    10021 gtttttgggt cattggctgt agaaaaaagc attgaaaacg tttcaggctt tattaacggc
    10081 acatcgccac aggcaacaat aacaatacca ttatatgcct gcaatacagg ttcggcctgc
    10141 ataactgcat gccctgttcc taattgttct ttttgccata caaatcgtgc tctgttacct
    10201 attgcctgta taacgtcttc acctctgtag ccaaccacca caatgatatc ttcttcttta
    10261 agacctgcat gtatcaaatt atcgataaca tgaagaacca gtggtttgcc attcaggtgg
    10321 tgcaatacct tgggcatttc agattgcatt cgtacgcctt tacccgcagc aaggactata
    10381 gctttacaat tactatccat acatggtacc aataaaataa aaaatctgag gtgccaggat
    10441 tcgaacctgg gaatgccggg accaaaaccc ggtgccgtac
//
LOCUS       DAOSZP010000003        10465 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_5415_length_10465_cov_3.263689, whole genome shotgun sequence.
ACCESSION   DAOSZP010000003 DAOSZP010000000
VERSION     DAOSZP010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 10465)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10465)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10465
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5415_length_10465_cov_3.263689"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..635
                     /gene="pyrH"
                     /locus_tag="PK348_00155"
     CDS             <1..635
                     /gene="pyrH"
                     /locus_tag="PK348_00155"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012963334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HXK64656.1"
                     /translation="PEIVSHIAREVKRAYELGVDVALVIGGGNIFRGSMGTSIGIERA
                     TGDYMGMLGTVINALAVQSVLENMKIPTRVMTAIEMRQVAEPYIRRRALRHLEKRRII
                     IFAAGTGNPFFTTDTAAALRAIEIGADLFLKATKVDGIYDKDPVKFPEAHKINAASYM
                     DVLQQQLGVMDYTAVSLCMENKLPLVVFNLFDEGSLVKILKGEDIGTRIS"
     gene            659..1216
                     /gene="frr"
                     /locus_tag="PK348_00160"
     CDS             659..1216
                     /gene="frr"
                     /locus_tag="PK348_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HXK64657.1"
                     /translation="MIKDILDDVKNRMQKSVQNLAKDFATIRTGRANPAIFDNVKVEA
                     YGTEMPLNQVATISCPEPRLVVIQPWDRTNLSAIEKAILKSNLSLTPNNDGNIIRIQI
                     PELTEERRKEYVKMIKQKAEECKVAIRNIRRDGNDMVKDLEKSKEISEDDAKNALDEI
                     QKITDKFVAEVQTMTDGKEKEIMSL"
     gene            1247..1969
                     /locus_tag="PK348_00165"
     CDS             1247..1969
                     /locus_tag="PK348_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009781585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HXK64658.1"
                     /translation="MLIHYKRKIDYSNIPRHVAIIMDGNGRWAKRRGLPRIEGHRRGA
                     ETIERCMDTAIELGINVVSLYAFSTENWSRPKEEINGLWNLLESFFEKKLNTIIEKGV
                     QVRHSGSLKELPVNVQRIITDAVEKSKNNSRIILNFCLNYGGRHEIIHAVNEWLQKKP
                     SPELSLTPEIFEGYLYTRGLPDVDLLIRTSGEYRISNFLLWQIAYAELVFMKVLWPDF
                     NEKHLYQAIYEYQHRERRFGGL"
     gene            1966..2853
                     /locus_tag="PK348_00170"
     CDS             1966..2853
                     /locus_tag="PK348_00170"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HXK64659.1"
                     /translation="MKERTKNTLIRILTAVVALPVYVFLIVTDSLYSLPVLLVSIIVT
                     GVCLWEFYQIAEGQTGYRPFVKTGMISALIINIIMYSYAYGNITGIGSLTVNFDVRYL
                     LAAVMIAISVFATIHIVKRPLKGGIYSLGATILGWFIIVIPYAHIMFLKSLRDGVLFI
                     IFVHLIVMINDTGAYFGGVYFGKHKANFKASPNKSWEGYFSGLLFGIVFAIIYSQLIE
                     VLYGRYFFSIVESAIIGIIFSIIGNIGDLFESAIKRDGAIKDSGSIIPGHGGMWDVFD
                     STIFAMPLFYYYLVIRGII"
     gene            2869..4023
                     /gene="dxr"
                     /locus_tag="PK348_00175"
     CDS             2869..4023
                     /gene="dxr"
                     /locus_tag="PK348_00175"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013075532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HXK64660.1"
                     /translation="MHSSVIIFGSTGSIGLSTLKVIRQFGNRFTVKGLSCHKNITLLK
                     EQILEFHPQYVAVSDEETCSTDEYKKLKKEFGSIVFYEGLEGLSKLAANGADILVVGI
                     VGAAALKPTLDSLQYIKRIAIANKETLVMAGDIFLKLLQRYAVELLPVDSEHCAIFTL
                     LGGRNTDSVKRLILTASGGSVRHKPVEALDTVTVEEALTHPTWDMGKKITIDSATLMN
                     KGLEVIEAHYLFSVPYESIEVMIHPESIIHGMVELIDGTLLAHMSITDMVYPIQRALT
                     YPQVCHNDFGNVDLTAIGSLHFTKPDKKRYPALELCYEAGRQGGTMPAVLNAANEVAV
                     NAFLQKKISFTNIVSVVEKVLGKHRKIDDPDLQTIFNADAEARQLALTCI"
     gene            4032..5594
                     /gene="rseP"
                     /locus_tag="PK348_00180"
     CDS             4032..5594
                     /gene="rseP"
                     /locus_tag="PK348_00180"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HXK64661.1"
                     /translation="MVTLTYILAAVILLGLCIFVHELGHLLGGKMVGIKAEVFSIGYG
                     KGFIKKKWGDTTYQITLIPFGGYCKFYGEDPNEERQGNPYEFLSAHPLKRVVTVLMGP
                     LFNLFFGIILFFIMNIYGYPVETNKIYIPEYLTKGEYVSPAYEAGLRTGDTIVAINNE
                     QVVGFTDIQSKVAFSKGKPITVKAIRGNDTLEVTIAPKKYTKKGYYTIGVMPYGDRVL
                     VMGILDNSPAKKAGLKEMDEIIEANGTQFHSPEEFTQFIRRHPDKELSLTISRGGHLI
                     TLPITPQTKEVLTINQFTDMRFPKESYTITVDSMSLIKAAANAGKVLINGKAVKSFEE
                     MQNIINASKGVITLKTPGGTYSGIMKFERYGFVGIETAISPENKFIQYGVRDAFVKSL
                     VDPFDFIALNFRGIGMMFSGELDVRQNLSGPIRIAKIAGDTAYYRGVSAFIILMAKIS
                     IILMVMNLLPIPMVDGSFILIFLYEAITRRAFSQKLLEKIQMIGFAILVLLGIFVIFN
                     DLSFLPFFKDLF"
     gene            5615..6697
                     /gene="ispG"
                     /locus_tag="PK348_00185"
                     /gene_synonym="gcpE"
     CDS             5615..6697
                     /gene="ispG"
                     /locus_tag="PK348_00185"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011025684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HXK64662.1"
                     /translation="MNYSRRKTIPVQAGSLTIGGDAPISLQSMTNIPVSQVKATIEKI
                     IRLQQEGAQLVRLALRTEDDAQYIKEIKKEVTVPLCADIHFNYKIALKAIENGIDKLR
                     INPGNIGAKENVVTLVKAASERNIPIRIGVNSGSVDIKKYGEVNPQTLVASAMEHVQL
                     LEDCRFTSIVVSIKSSDLLQTIEANRIFASQRQYPIHIGLTEAGYGTDCIIASSMVIG
                     TLLYEGIGDTIRLSMTGDPVHEVRAAKRILEIGGFRFRPYTIIACPTCGRTDTKLDIL
                     QLTQEVESMVKLHFEKTLIEKHKHITIAVMGCEVNGPGEARHADIGIAGARDGRVVLF
                     ARGEKKKILKASEAIKAIEEEIDAMV"
     gene            6771..7019
                     /locus_tag="PK348_00190"
     CDS             6771..7019
                     /locus_tag="PK348_00190"
                     /inference="COORDINATES: protein motif:HMM:NF039417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6364 family protein"
                     /protein_id="HXK64663.1"
                     /translation="MNSKLTLYIDKKVITQAKQYARKNKTSISEVVEKFLESLVDNHN
                     KYTIAPITKELSNIIVKKPKKINYKREIEDYLLFKYDL"
     gene            7133..7564
                     /locus_tag="PK348_00195"
     CDS             7133..7564
                     /locus_tag="PK348_00195"
                     /inference="COORDINATES: protein motif:HMM:NF024861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HXK64664.1"
                     /translation="MKNKLFIDTDVILDVALRRHPYYINSALVLSLIELKEFHGFTST
                     VIITNLYYILRKLESHDIAVEFIKKLRLIIDVLAVDDEIINLALESKFKDFEDAIQYY
                     TALKNGVKYIITRNTKDYSSGNIIACTPDEFVTIIETKHKE"
     gene            7574..8368
                     /locus_tag="PK348_00200"
     CDS             7574..8368
                     /locus_tag="PK348_00200"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HXK64665.1"
                     /translation="MCSSFCYHIPDGLYTIGITQNSIETLLPYLPPDIKPEYLYHSFP
                     QHTVALQQFAIAMRYVTVSEFAQFIEETGFITQAQKDGWSWTWEKGWTKKQGLWWQKP
                     FGIDELDTLYTEHSSSMCVLQVSCNDARAYCEYLSKKTGEVIMLPTEFMWEAFAAKVG
                     IPGMEQLQELSPNKHVLLSDCCHSRMELTKQRQVLPGTVWEWTDSYFQGYPGCLPHKE
                     YGTTYKVLRGGSYFSSPLQRAREYRFRRCPTARSPFYTFRICVVPA"
     gene            complement(8378..9136)
                     /locus_tag="PK348_00205"
     CDS             complement(8378..9136)
                     /locus_tag="PK348_00205"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014165644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HXK64666.1"
                     /translation="MKIIDLSSTIEPSPPQTPDFLKIDVQYHSHADGAKQVEALLKVP
                     AHLMRNNEGWATETITRLGTHDSTHVDAPWHYNTTIGGKPAPTIDELPLEWFYGNGVK
                     LDMTHKPEGNPVTPEDIENELQRIKYSIKPNDIVLIQTGMDKYYGQPDYIFRGCGVTY
                     EATVMLYNFGVRVMGIDAWGWDMPLNLQAQKALEANKPGIFWAAHQADLVYSHMERLV
                     NLDKVPSTGFKVACFPLKIKRASAAPARVVAIIE"
     gene            9440..>10465
                     /locus_tag="PK348_00210"
     CDS             9440..>10465
                     /locus_tag="PK348_00210"
                     /inference="COORDINATES: protein motif:HMM:NF015573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HXK64667.1"
                     /translation="MKLPFTFDAMLAFGWMSIMLLLGIILRANIKAFQRFLIPSCLIG
                     GAIGLVLRSYEIIPITADILETLAFHLFNISFISIGLTYNKEKADLKRAGEVLQGSIW
                     MANVEGISFAIQAIAGGLCVMLLNSIGFNLFPAFGFFTPLGFTEGPGQALSVGKVWEN
                     LGYAHSATLGLTFAVFGFLFAFFVGVPLANWGIKKGMAVHTPKELSDEIRKGIIQPQS
                     EKEIAGELTTHSSNVDAMAFQASIMGLVYMITYAVVYKVASVLSPEMGQMVWGFFFFI
                     GLVIAFIVRLIISKMGGLYLIDPGIQRRITGFAVDYLLVATIMAIQVVVVWQYIIPIL
                     VISIACGL"
BASE COUNT         3209 a         1763 c         2281 g         3212 t
ORIGIN      
        1 atcctgagat tgtttcccac attgcccgtg aagtgaagag agcctacgaa ttgggagttg
       61 atgttgcact tgtcattggt ggtggtaata tcttcagagg gtcaatgggg acatccattg
      121 gcattgagcg ggcaaccggc gattacatgg gaatgcttgg aacagtgatt aatgcgcttg
      181 ctgttcagag cgttcttgaa aacatgaaaa taccaacacg tgtgatgact gctattgaaa
      241 tgcgccaggt tgcagaacca tatatacgca ggcgggcatt gcgtcacctg gaaaagcgaa
      301 gaattattat ctttgctgct ggtacaggaa atccgttttt tactaccgat actgctgctg
      361 cattgcgtgc cattgaaatt ggtgcagacc ttttcctcaa agctaccaaa gttgatggga
      421 tttacgacaa agacccggtt aaattccctg aagcgcataa aataaatgca gcatcataca
      481 tggatgtttt acagcaacaa ttaggggtaa tggattacac tgcagtgtca ttatgtatgg
      541 aaaacaaact accacttgtt gtatttaatt tgtttgatga aggatcactg gtaaagattt
      601 taaaaggtga agatataggc acacggattt cgtaatatta atttggttgg gggaatatat
      661 gataaaagat attcttgatg atgtgaaaaa ccgcatgcag aaaagtgttc aaaatctggc
      721 aaaggatttt gcaacaatcc gtactggcag ggcaaatcca gcaatctttg ataatgttaa
      781 ggtagaagcc tatggtaccg aaatgccatt aaatcaggtt gctacaatat cctgccccga
      841 gccacggctt gtagtaatac agccatggga cagaaccaat ttaagtgcta tagaaaaagc
      901 aatattaaaa tccaatcttt ctttaacccc aaataatgat ggcaatatca ttcgtattca
      961 aatacctgaa cttaccgaag aacggcgcaa ggaatatgtt aagatgataa agcaaaaggc
     1021 agaggaatgc aaagttgcta tacgtaatat tcgccgtgac ggcaatgaca tggtaaaaga
     1081 ccttgaaaag tccaaggaaa tatcagaaga tgacgcaaaa aatgcactgg atgaaattca
     1141 aaaaatcact gataaatttg tagctgaagt acagacaatg actgacggca aagaaaaaga
     1201 gataatgagc ttgtaacttt tcaagcccat tagtatactt attaatatgc ttatacatta
     1261 taaacgaaag attgattact ccaatatacc acggcacgtt gctatcatca tggatggcaa
     1321 cggaagatgg gcaaaaagaa ggggcttgcc gcgtattgag ggacacaggc gtggtgccga
     1381 aaccattgag cgctgcatgg atactgcaat agagcttggc attaatgttg tttcactgta
     1441 tgcgttttca actgaaaact ggtcacgccc aaaagaagaa attaatggcc tgtggaatct
     1501 tctggaatct ttctttgaaa aaaagctgaa caccataatt gaaaaagggg ttcaggtacg
     1561 gcattctggc agtcttaagg agcttcctgt aaatgttcag cgcattatta ctgatgctgt
     1621 tgaaaaatca aaaaacaatt ccaggataat acttaacttt tgcctgaatt atggcggaag
     1681 acatgagatt attcatgcgg tgaatgaatg gctacagaaa aaaccttccc cggagctatc
     1741 gcttacccct gaaatatttg aaggctattt atataccaga ggattaccgg atgttgatct
     1801 tttaatccga accagtggtg aatacaggat aagcaatttt ttattatggc agatagcata
     1861 tgcggaatta gtatttatga aggtattgtg gccagatttt aatgagaaac atctctacca
     1921 ggcaatatac gaatatcaac acagggaaag gaggtttggt ggcttatgaa agagcgcact
     1981 aaaaatactt taatacggat tctgactgca gttgttgcat tgccggttta tgtgtttttg
     2041 attgtaacgg actcattata ttcattgccg gtgcttctgg ttagcatcat tgttaccggt
     2101 gtttgcctgt gggaatttta tcagatagct gaagggcaaa ccggttacag gccatttgta
     2161 aagacgggaa tgatttcagc attgattatc aatattatca tgtattcgta tgcatatgga
     2221 aacattaccg gtattggcag tttaacagta aattttgatg tacgctatct gcttgcagct
     2281 gtaatgatag ctatcagtgt gtttgctaca attcatattg taaagcgccc attaaaaggg
     2341 gggatatact cattaggtgc aactatactt ggctggttta tcattgttat tccgtatgca
     2401 catataatgt ttttgaaatc attacgtgat ggtgttctat tcattatttt tgtacatctg
     2461 attgttatga ttaatgatac cggtgcatat tttggaggtg tgtattttgg gaagcataaa
     2521 gccaatttta aagcatcgcc caataagtca tgggaagggt atttcagtgg acttttgttt
     2581 ggcattgttt ttgcaattat ttacagccag ctaattgaag tattgtatgg tagatatttc
     2641 ttcagtattg ttgaatcagc cattattggt attatattca gtattatagg taatattggt
     2701 gatttgtttg aatcagcaat aaaacgtgat ggtgccataa aagattcagg ttctattatt
     2761 ccaggtcatg gtggtatgtg ggatgtgttt gattcaacga tatttgcaat gccattattt
     2821 tactactatc tggtaatccg agggattata taaggtactc attggtttat gcattcaagc
     2881 gtaataatat ttggttccac cggttcaata ggcctttcta ctcttaaagt gattcgtcag
     2941 tttggaaaca gatttactgt aaaagggcta tcgtgccata aaaatataac actattaaaa
     3001 gagcagatac ttgaatttca tccacaatac gttgctgtaa gtgatgaaga aacgtgttct
     3061 actgatgaat ataaaaagtt aaaaaaagaa tttggttcga tagtttttta tgaagggctt
     3121 gaaggcttaa gcaagcttgc tgcaaatggt gctgatattc tggttgttgg cattgttggt
     3181 gctgctgcac tcaagccaac acttgattcg ttacagtata ttaaacgcat agcaattgcc
     3241 aataaagaga cactggtgat ggcaggtgat atttttttaa agcttttaca gcggtatgct
     3301 gttgaacttt tgcctgtgga cagtgaacac tgtgctatat ttacattgct tggtggcaga
     3361 aatactgatt cagtaaagcg gttaatactg acagcaagcg gtggaagtgt gcgtcataaa
     3421 ccggttgagg cgcttgatac tgttactgtg gaagaagccc ttacgcaccc aacatgggat
     3481 atgggtaaaa agattaccat agattctgca acattgatga acaaggggct tgaggttata
     3541 gaggcacatt accttttttc agtgccgtat gagtcaattg aggtgatgat tcatccggaa
     3601 agcattattc atggtatggt ggaactcatt gatggcacat tgcttgctca tatgagcatt
     3661 accgatatgg tgtatcctat ccagcgggca ttgacgtatc cacaggtgtg ccataatgat
     3721 tttgggaacg ttgatttaac agcaataggt tccctgcatt ttacaaaacc tgataaaaag
     3781 cgctatccgg cacttgagct gtgttatgaa gctggaaggc agggtggtac catgccggca
     3841 gtactcaatg cagcaaatga ggtagccgtc aatgcgtttt tacagaagaa gatttcgttt
     3901 actaacatag tatcagtagt tgaaaaggtt ttagggaagc ataggaagat tgatgatcct
     3961 gatttgcaaa caatattcaa tgctgatgca gaagcacgac aattagcact aacatgtata
     4021 tgaggtaaat aatggtaaca cttacgtata ttttagcagc tgtaatttta ctgggtttgt
     4081 gtatttttgt tcatgagttg ggacacctgt taggtggcaa aatggtgggc ataaaggcag
     4141 aagttttttc cattggttat ggtaaaggtt ttataaagaa aaaatggggc gatacaacct
     4201 atcagataac attaatccca tttggtggat attgtaaatt ttatggtgag gatccaaacg
     4261 aagagcgtca aggcaatccg tatgaatttt taagtgcaca tcccttaaag cgtgttgtta
     4321 cggtactcat ggggccactg tttaacctgt tttttggtat catactcttt tttattatga
     4381 atatctacgg ctatcctgtt gaaacaaata aaatctatat ccctgaatat ttaacaaaag
     4441 gtgaatatgt ttctcctgcg tatgaagcag ggcttaggac cggggatact atcgttgcta
     4501 ttaacaatga gcaggttgtt gggtttaccg atattcagag caaagttgcc ttttccaaag
     4561 gcaaacctat cacggttaaa gcaatcaggg gcaatgatac attggaagta actatcgccc
     4621 caaaaaaata cacaaagaag ggatactata ccattggtgt tatgccctac ggcgataggg
     4681 tgctggttat gggtattctt gataattccc cggcaaaaaa agctggttta aaggaaatgg
     4741 atgagatcat tgaagcaaat gggactcagt tccactcacc tgaggaattc acacaattta
     4801 tacggcgaca tcctgataag gaattatcgc ttaccataag tagaggtggg cacctcatta
     4861 cacttcccat aactccacaa acaaaagagg tgttgacgat aaaccagttt acagacatgc
     4921 gctttccaaa agaaagctat acaattacgg ttgattcaat gtcgctgatt aaagcagcag
     4981 caaatgctgg aaaggttctc ataaatggaa aagcagtaaa gtcatttgaa gaaatgcaaa
     5041 acattattaa tgcttcaaaa ggtgtcataa cacttaagac tcctggtggc acctatagtg
     5101 gcatcatgaa atttgaacgg tatggatttg tgggtataga aactgctatt tcacctgaaa
     5161 ataaatttat acagtatggt gtacgtgatg cttttgtaaa atcgctggtt gatccatttg
     5221 attttattgc acttaatttc cgcggcattg gcatgatgtt ttcgggtgag cttgatgtgc
     5281 ggcaaaattt atcaggtcca atacgcatag caaagattgc tggcgatact gcatactatc
     5341 gcggcgtgtc tgcatttatc atactcatgg caaaaatttc catcatcctt atggtcatga
     5401 acctgcttcc cattcccatg gttgatggaa gctttattct catctttctg tacgaagcca
     5461 ttacgcgccg tgcattcagc cagaagctat tagaaaaaat tcaaatgatt ggttttgcca
     5521 ttctggtgct tttgggtata tttgttatat tcaatgattt atcgtttttg ccatttttca
     5581 aagatttatt ttaatgtgcg atagttactg attaatgaat tattcccgca gaaaaaccat
     5641 tcctgtacag gcaggcagtt taaccattgg tggtgatgca ccgatatcgc tgcagtcaat
     5701 gacaaatata cctgtaagcc aggtaaaagc aacaattgag aagataatac gtttgcagca
     5761 ggaaggggct cagcttgtac ggcttgcatt gcgtacagag gatgatgcac agtatataaa
     5821 agaaataaaa aaagaagtaa ccgtaccatt gtgcgctgat atccatttca attacaaaat
     5881 agcactgaaa gccatagaga acggcataga taaattgcgc attaatcctg gcaatattgg
     5941 cgcaaaagag aatgtggtta ctttagtgaa ggctgccagt gaaagaaata tccctatcag
     6001 gattggtgtt aactcaggtt ctgtagatat aaaaaaatat ggggaagtaa atccccaaac
     6061 gcttgttgct tcagccatgg agcatgtgca gcttcttgaa gattgcaggt ttaccagtat
     6121 agttgtttcc ataaaatcat cggatttgct gcagacaatt gaagcaaacc gcatctttgc
     6181 atcacagcgc cagtatccca tccatatagg attaaccgaa gctggctatg gcactgattg
     6241 tattattgca agcagtatgg ttattggcac actactatat gaaggtattg gcgataccat
     6301 acgactttcc atgacaggtg atcctgtcca tgaggtaaga gctgcaaagc gcatccttga
     6361 aattggcggt ttccgattca ggccatatac cattattgcc tgcccaacat gtggccgcac
     6421 cgatacaaaa ttagatatac tgcaattgac gcaggaagta gaatcaatgg taaaattaca
     6481 ttttgaaaag acattgattg aaaagcacaa acacataacg atagcggtaa tgggatgtga
     6541 ggttaatggc cccggtgaag cacggcatgc agatattggc attgctggag cacgtgatgg
     6601 cagggttgta ctgtttgcaa gaggtgaaaa gaaaaaaata ctgaaagctt ctgaagctat
     6661 aaaggcaata gaagaagaaa tagatgcaat ggtgtagtaa ttaaattatt acttgacata
     6721 tacgtattgt ttttatattt tattacgtag atatttataa ggtttgaact atgaattcaa
     6781 aattaacatt atacattgat aagaaagtga taacacaagc aaaacagtat gcgcgtaaaa
     6841 ataaaaccag tatttcagag gtagttgaaa aatttttaga atcattggtt gataaccata
     6901 ataaatatac aatagcacct attacaaaag aattatctaa tattatagta aagaaaccaa
     6961 aaaaaattaa ttataagcgt gaaattgagg attatttgct tttcaaatat gatttgtaat
     7021 aaaagcacta tttttttcaa taacaatgtc tttaaaaaaa tgtatatttt gatagttaaa
     7081 gacgcatgcg tttttttata atcaaacaaa aatttaaatc aatcatcagt gcatgaagaa
     7141 taaattattt attgatacag atgttatttt ggatgttgcg ctgagacgtc atccttatta
     7201 tatcaattca gcacttgttt taagcctcat tgaattaaaa gaattccatg gtttcacctc
     7261 aacagtaatt ataacaaatc tttactacat tcttagaaaa ttggaatcac atgatatagc
     7321 cgtagaattt attaaaaaat tgcgtttaat tattgatgtt ctggctgtag atgatgagat
     7381 tataaatctg gcattagaat caaagtttaa agattttgaa gatgctatac aatattatac
     7441 tgcgttgaaa aatggtgtaa aatatatcat aaccagaaat acaaaggatt attcatcagg
     7501 gaatattatt gcctgtacac ctgatgaatt tgtaacaatt attgaaacta aacataagga
     7561 atagagattg ctaatgtgtt cctctttttg ctatcatatt cctgacggct tgtataccat
     7621 tggtattaca caaaattcta ttgagacatt gttaccttat ctgccacctg atataaaacc
     7681 tgaatacctg taccattcat ttccgcaaca cacggtagca ttgcaacagt ttgccatagc
     7741 aatgcgctat gtgaccgtga gtgaatttgc tcagtttatt gaggaaaccg gttttattac
     7801 gcaggcgcaa aaggatgggt ggtcatggac atgggaaaag ggctggacta agaaacaagg
     7861 cctgtggtgg caaaagcctt ttggcattga tgaacttgat acactgtata ctgaacacag
     7921 tagcagtatg tgtgtgttgc aggtaagctg caatgatgcc cgggcatact gtgagtatct
     7981 gtcaaaaaag acgggggagg tgattatgct tcccacggag tttatgtggg aggcttttgc
     8041 agcaaaggtt ggtataccag gtatggaaca attgcaggaa ctatcgccca ataaacatgt
     8101 actgcttagc gactgctgcc acagccggat ggagcttaca aaacaacgcc aggtgttgcc
     8161 cggtactgtt tgggaatgga ccgatagtta ctttcaagga tatcctggtt gcctgccaca
     8221 taaagaatat ggtaccactt ataaggtact gcgtggtggc tcatatttta gcagtccatt
     8281 gcaaagagca cgggagtacc gattcagacg ttgccccact gcacgaagtc cgttttacac
     8341 cttcagaata tgtgttgtgc cagcttgatg aaagcagtta ttcaatgatg gcaaccacgc
     8401 gtgctggtgc ggcacttgcc cgtttaatct ttagcggaaa acaggcaacc ttgaatcctg
     8461 ttgaaggcac tttatctaaa ttgacaagcc gttccatatg cgagtataca aggtcagcct
     8521 gatgggcagc ccagaatatg ccaggcttat ttgcttcaag tgccttctgt gcttgcagat
     8581 tgagtggcat atcccagccc cacgcatcaa ttcccatcac acgaacacca aaattgtata
     8641 acattactgt tgcttcatac gtaacgccgc aaccacggaa gatataatca ggctgtccgt
     8701 aatacttatc catgccagtt tgtatcagga ctatatcatt gggctttatt gaatacttga
     8761 tgcgctgtaa ttcattttca atatcctcag gtgttacggg attgccttca ggcttatggg
     8821 tcatatctaa tttaacacca ttgccataaa accattccag tggcaattca tcaatagttg
     8881 gtgcaggttt tcctccaatg gtggtgttat aatgccaggg tgcatcaaca tgggtgctat
     8941 cgtgtgtgcc aaggcgggta atggtttcgg ttgcccagcc ttcattatta cgcataagat
     9001 gtgcaggcac tttcagaagt gcttccacct gttttgcccc atcagcatgg gagtgatact
     9061 gtacatcaat ctttaaaaaa tcgggagttt gtggtggtga aggttcaatg gttgatgaaa
     9121 gatcaattat tttcatggct acctccagat gcaatatgta gtgtttagtg gttattgaat
     9181 gtagtgaaaa aatgtggtta atgcaaacat ttttttacta tatataaagg tttgtctatg
     9241 tctgtgcttt atgattttta gtattaccag atggaatttt tatagatgcc aaagagtttg
     9301 atgtgtggaa tcactgttta cattacatta aaaataaatt attaattgac atcgttttaa
     9361 atatgattat ataatcatat cacagcaagg gggtcagagc ctatagataa aaatatacat
     9421 taaaaaaatg ggggatgcca tgaaactgcc ttttacattt gatgctatgc ttgcttttgg
     9481 atggatgtca ataatgctac ttttaggcat tattctgcgt gcaaatatta aagcatttca
     9541 aaggtttctt atcccaagtt gtcttattgg tggtgcaata gggcttgttt tacgaagtta
     9601 tgagattata cctattactg ctgatattct ggaaacgtta gctttccacc tgtttaacat
     9661 atcatttatc tctattgggc taacgtacaa taaagaaaaa gcggatttga agcgtgctgg
     9721 tgaagttttg cagggttcaa tatggatggc aaatgttgaa ggcatatcat ttgcaataca
     9781 ggcaattgct ggtggattat gtgttatgct tttaaattct ataggattta accttttccc
     9841 tgcttttggc ttttttacac cgttaggttt tactgaaggg ccagggcagg cattatcggt
     9901 tggcaaggtt tgggaaaatt taggatatgc acattcagct acattgggtc ttacttttgc
     9961 tgtatttgga ttcctgtttg ctttttttgt tggagtgcct ctggcaaact ggggtatcaa
    10021 aaaaggtatg gcagtacata ctccaaaaga attatctgat gaaatccgta aaggtataat
    10081 acaacctcag agtgaaaaag aaatagccgg tgagcttacc acacattctt cgaatgttga
    10141 tgcaatggca tttcaggcaa gcatcatggg gctggtgtac atgattacct acgctgttgt
    10201 ttataaagta gccagtgtat taagtcctga aatgggtcag atggtttggg gatttttctt
    10261 cttcattggg cttgtcatag cttttattgt acggcttatc atttcaaaaa tgggaggatt
    10321 atatctcatt gaccctggta ttcagcggcg tattaccggg tttgctgttg attatcttct
    10381 tgttgccacc atcatggcaa ttcaggttgt ggttgtgtgg caatacatta ttcctattct
    10441 ggttattagt attgcatgtg gttta
//
LOCUS       DAOSZP010000004         9789 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_5903_length_9789_cov_3.471338, whole genome shotgun sequence.
ACCESSION   DAOSZP010000004 DAOSZP010000000
VERSION     DAOSZP010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 9789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9789
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5903_length_9789_cov_3.471338"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..490)
                     /locus_tag="PK348_00215"
     CDS             complement(<1..490)
                     /locus_tag="PK348_00215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK64668.1"
                     /translation="MIKPVVLDFEYNVQSTQNIKNNVPELSFSSILKDAMQNTSEATD
                     PECNQTDDTVNHYSNSATVQVKDTKEKDYDRVQDKSKNGERTFADRRNEQNNEVYVEK
                     QDVMQSLAGSALHTNNNRAIVTDKNALTEKHSVYKKDSYRTLKLHTINNNKEQKLFAN
                     ETI"
     gene            1277..2545
                     /locus_tag="PK348_00220"
     CDS             1277..2545
                     /locus_tag="PK348_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004074731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HXK64669.1"
                     /translation="MEAIESLQNTEIQEPPDFSTSNTDEPPSACNTQSNVITILPSRH
                     VVHKPSTSNKTKIFRKLFYPDIIDSQWNDWRWQIANRIRTYEELSAILELTENEKNAL
                     ISHTQLPMSITPYYASLIERNNPFDPIRRCVVPTIHELEHNHGESEDPLSEDDDSPVP
                     GLVHRYPDRVLFLATDFCSTYCRYCTRSRIVGRSNCTLPGKNWDMALRYIAEHTEVRD
                     VLISGGDPLTMNDSMIEYLLNRLRAIPHVEIIRIGTKVPAVLPQRITHLLVSMLRKYH
                     PLWINIHFTHPDEITPETMKACNRLANAGIPLGSQTVLLKGINDNVETLKKLYHLLLQ
                     CRVRPYYLYQCDPIVGSQHFRTSVAKGLEMIEGLRGHTTGLAVPHYVIDAPGGGGKVP
                     LLPTYYQGMEGDSVVLKNYEHKYFYYPETT"
     gene            2557..3576
                     /locus_tag="PK348_00225"
     CDS             2557..3576
                     /locus_tag="PK348_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015903222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HXK64670.1"
                     /translation="MRVGITYDLRDDYRAMGYDEEAIAEFDSIETINAIDTTLQQLGF
                     ITERIGHIKHLVSRLASGQRWDIVFNIAEGIHGIGREAQIPALLDAYTIPYTFSDPLV
                     LALTLNKAMTKQIIRDSNIPTPPFMLVTGIDDIKRCSLAYPLFAKPYAEGTGKGITAS
                     SVIRDAGELELTCTQLLQKYKQPVLVEEYLPGREFTVGIVGSGNNAKTIGTIEIILLD
                     NAEKDVYSFINKEQCEERVEYRLADDITAQKSADVALKAWRALGCLDAGRVDIKCDSS
                     GNPHFLEVNPLAGLHPTHSDLPIICSKAGILYQQLLATIMHNALTRYNMLDNAPQKLS
                     SLAIK"
     gene            3686..4693
                     /locus_tag="PK348_00230"
     CDS             3686..4693
                     /locus_tag="PK348_00230"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HXK64671.1"
                     /translation="MDIIFIHQYVPDDAPLDEKDVLEQRDELAEIFIQMGYTVGSRAI
                     QSHLDLLSLQELSRSSCIVFNLCESLFGRDDLMFLAPFMLEQYGIPFTGSGSHAIALS
                     CHKLESKKLMMQYGIPTPAYITGNTEYHETLNGTYIIKPYNTHASINITQDSIVQIQS
                     RSDFNVLKEKLMASPNLFAEEYIDGRELNISLLLTDDGVQILPHAEIHFDNYPPDKYK
                     IVDYEAKWIHNSFAYENTNRSFSFTEEDRPMLHEIDRISFKLWDIFKLNGYARIDFRV
                     KDKEVYVLEVNANPCISIYSGFMAAVEEYGLTFAQALDIIIKHPVIPKKHLCEADEAC
                     N"
     gene            4677..5177
                     /locus_tag="PK348_00235"
     CDS             4677..5177
                     /locus_tag="PK348_00235"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXK64672.1"
                     /translation="MKHVIKNLIQYRDFPVQTDCDTVVSILDATGFFYPEEIDIALEL
                     LNEKINYPDSSSYEFIFAEYQTTVVGYICYGRIPLTKASWDVYWIAVHPDFQGNGIGY
                     TLLSKAEETIKLHNGKHFYIETSGRKLYEPTHTFYLKAGYTVSAVFKDFYADGDDKLV
                     FYKKLV"
     gene            5343..5576
                     /locus_tag="PK348_00240"
     CDS             5343..5576
                     /locus_tag="PK348_00240"
                     /inference="COORDINATES: protein motif:HMM:TIGR03831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiT-type zinc finger protein"
                     /protein_id="HXK64673.1"
                     /translation="MECSCGGILTEGRSSYSVSGQHFYLVIEDIPAYKCNRCGKILFE
                     DDVVDKIKALLKRVERDTKEIVTGTPSVHTYDY"
     gene            5606..7042
                     /gene="gltX"
                     /locus_tag="PK348_00245"
     CDS             5606..7042
                     /gene="gltX"
                     /locus_tag="PK348_00245"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008517613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HXK64674.1"
                     /translation="MRVRFAPSPTGFLHVGNARTAVLNFLFKKKYNATYILRIEDTDM
                     ERSTRESEESILNDLKWLGIQWDEGPDCGGTFGPYRQSERFDIYREYTEKLLQGGHAY
                     HCYCTQEELDAQRQKAAQDNKPFVYPGTCRDLTLEQKLAFEKQGRKPTVRFKVKDGIT
                     LSIDDQIKGNVVFNSENIGGDFIIVRSDGVPVYNYIVIIDDTLMNVTHVIRGEDHLPN
                     TPKQMLIAMALGLPVPVYAHMPLLLGPDRTKLSKRHGITSVAVYREQGYLPQALFNYL
                     ALLGWTLESGEEIVPVERLVAEFELSKIGKSSAIFDFQKLKWMNSVYLRNLPFDKAFE
                     LFYPYIEKAYPTVSNSEWLTQVVDTVRGNCEIASDIVKEISIFFGDTAEFEEDAKNML
                     ATPEGKTIIQTAYELIHSTINESNYSTETLNTIKQKTGLKGKMLFMPFRAMVTGRLHG
                     PELDKILPLLGYENFTKRIEYCHQRFVK"
     gene            7305..7994
                     /locus_tag="PK348_00250"
     CDS             7305..7994
                     /locus_tag="PK348_00250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK64675.1"
                     /translation="MKKLGSTIIVFTLLTVCACSAHAPGTIPVGTWQYDLLVNGVEMG
                     KATIQNSIEDNQYVYRFDLTIGNGTTRSQDTIVETLECKPIKLEKHTYIKQTTTEQKM
                     DIIAQCNGRTIILIENNMKSIITLDQDYVLDGNYFLYMLIQNKFKKGLQITHNVYDPS
                     IEREAAIPVTIKLIGTETISVNSILYKAWKIEQSIGKIKNIIVYLDEHGVVLKSSIKM
                     LNMQLDIIRKD"
     gene            7997..8776
                     /locus_tag="PK348_00255"
     CDS             7997..8776
                     /locus_tag="PK348_00255"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HXK64676.1"
                     /translation="MKKIYAIVISAIVGGYLLYLYSQPLPASLSTVPIANITSKLWEE
                     NISLFMVSSDEAFSITQTPFQKILSSRNKNTSYAYIIASGKNKEYHQNPVEKDLKDTR
                     LLQIHNPVIVQHAKTIASSSNPIEEAEELVYNTIETKTEGIPIIPAPAIFELKKGDCT
                     EHAIATIALLRALHIPARAVVGMIAAKEYAGKKNVFVFHMWAEAFYNNAWLLVDATRP
                     GKKKTNIYIALTYHSLETEMPLEFLEAMANIQNLKVTYLSE"
     gene            complement(8757..>9789)
                     /locus_tag="PK348_00260"
     CDS             complement(8757..>9789)
                     /locus_tag="PK348_00260"
                     /inference="COORDINATES: protein motif:HMM:NF015013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HXK64677.1"
                     /translation="LLLNVGIILFCFIAYLYSNVIWLGVGVVAGGILQCVVVFIPLYK
                     TGVHLTLGHIQSGHTKVLRMIVASVMGVAVYHINVLVNTMMASTLKQGSVSYLYYADR
                     FIEIVSGVFIISMSNAFHPEVSRLWAGRDVARLQATMTSMLQASLFIALPASAGLIAT
                     GKVILATFFEHGNFYSFETAMTYKALACAAFGIPAFAVVRILMPLFISMRSSVFINVI
                     STVAIILNAVLGFVLMRTHLLHGGLALANSISLYCVAIALVIKLLHSIKVKVDKRFTV
                     TLLQYFIASGIMGISLYILQSFLPYEKGLTYTVVLVTLGVVLYAILCKLINPGMTLRI
                     LLDAIQKDM"
BASE COUNT         3184 a         1942 c         1813 g         2850 t
ORIGIN      
        1 caatggtttc gtttgcaaat aatttttgct ctttattatt gtttatagta tggagtttca
       61 aagtgcgata tgaatctttt ttgtatacac tatgtttttc agtaagggca tttttatcag
      121 taactatcgc cctgttatta tttgtatgta atgcagaacc tgccaatgat tgcatgacat
      181 cctgtttttc cacatacact tcattatttt gttcattccg cctgtctgca aaagttcttt
      241 ctccattctt tgatttatcc tgcactctat cataatcttt ttctttagta tctttcacct
      301 gcactgttgc actatttgaa taatggttca ctgtatcatc tgtctggtta cattcaggat
      361 ccgttgcttc actggtattt tgcattgcat ctttcaatat agaactgaac gatagctccg
      421 gtacattatt tttgatgttc tgcgtactct gtacgttata ctcaaaatcc agcacaacag
      481 gttttatcat atttataccc tgtaataaga tgaagtctta ccattaatat cgaatgaccc
      541 gtttttttta atgaattaat taagcaatta ttacaaaaaa atatttttat gaacgaaaga
      601 acaacacttt tgcattatgt catattagct gttattttta accttaattt caagaggact
      661 taaaatccta atataacacg ctgtatcaag aattcttctc tcttcgtttt taaaaccgac
      721 attgcaaaaa aaatgttatt ctaaatgaca tcaagatgac cgcttgtggc tatcaaatac
      781 acaaaattag ccaaaataaa aatattaaaa ttttaatata aaaatataaa aaattttagt
      841 tgcaatacta tacgtgtgat atatatggta ctgtaacttc tgcgctatta cctgcaatgt
      901 ttaacgcagg tttatagcgg cattatttgg aatagaactt tccataattg ccgatcctcc
      961 attttttatg gatcccagac tcacgtcccc aaaatgggag acctcttgga cactcatccc
     1021 tgtcaaaagc agggatgctt tttttatatt tttttatttt ttttctttac atgctgtata
     1081 tttgttttta attgcatttt attttttagt atatatgccg attcaaatac tataaatttt
     1141 atagtagaaa aatgtaattt ttttgcgaca taatacggta tttttcttga caaaatactt
     1201 tttttatatt tgtaagaaat agattataca ttgtgattta aaaatcttct tttgataaag
     1261 tgaggtacgc aaatagatgg aagcaattga atcactgcaa aatacagaaa tacaggagcc
     1321 tccggatttt tccacctcaa acactgatga gcctccctcg gcgtgtaata cacaatcaaa
     1381 tgtgataaca atcctgcctt cacggcatgt tgttcataaa ccttccacaa gcaataaaac
     1441 aaagattttc agaaagcttt tttatcctga tatcattgat tcacaatgga atgactggcg
     1501 gtggcagatt gccaacagaa tacgtacata tgaagaacta tctgcaattc ttgaactcac
     1561 tgaaaatgaa aagaatgcgt taatcagcca cacgcagtta cccatgtcaa taacacccta
     1621 ttatgccagt cttattgagc gcaacaatcc ttttgaccca attcgccgct gtgttgttcc
     1681 aacaattcat gaactggaac ataatcacgg cgaatctgag gacccgcttt cagaagatga
     1741 cgacagcccg gtcccgggat tagtgcatcg ctatcccgat agagtgttat ttttagcaac
     1801 cgatttctgt tcaacatatt gccggtactg cacccgttca cgcattgttg gtcgctcaaa
     1861 ctgtacacta ccaggtaaaa actgggacat ggcattacgc tatattgctg aacataccga
     1921 agtccgtgat gtacttattt ctgggggcga ccctttgaca atgaatgatt caatgataga
     1981 gtaccttctt aaccgtctca gggccattcc acacgttgaa attatccgca ttggtactaa
     2041 agttccagcg gtgttgcctc agcgtatcac ccatttactg gtaagcatgt tacgaaaata
     2101 tcatccatta tggataaaca tacactttac ccatcctgat gaaataaccc ctgaaaccat
     2161 gaaggcttgt aacaggcttg caaatgcagg tattccttta ggaagccaga cagtgctgct
     2221 aaaggggatt aatgataatg ttgaaactct gaaaaaactg taccatctgc ttttacaatg
     2281 cagggtacgc ccctattacc tgtatcagtg tgaccccata gtaggttcac agcatttccg
     2341 cacttcagtt gcaaaaggtc ttgaaatgat tgaagggctc aggggacata caaccgggct
     2401 tgcagttcct cactatgtta tagatgcacc gggaggtgga ggtaaagtac cactgttgcc
     2461 cacatactat cagggtatgg aaggggattc tgtggtcctt aaaaattacg aacataaata
     2521 tttctactat cccgaaacaa catgaccagc agagccatgc gtgttggcat tacctatgac
     2581 ttacgagacg attaccgggc aatgggctat gatgaggaag ctattgccga atttgacagt
     2641 atagaaacaa ttaatgccat agataccacc ctgcagcaat taggttttat caccgaaaga
     2701 attggccata ttaaacatct tgtttcacgc cttgccagcg gtcagcgctg ggatatagta
     2761 ttcaatatag ctgaaggtat acacggtatt gggcgtgaag cgcagattcc agcactactt
     2821 gatgcctaca ccattcccta caccttttct gacccacttg tgctggcact gacattaaat
     2881 aaagccatga caaaacagat aatacgcgat agcaatatac ccactccccc atttatgctt
     2941 gttaccggta ttgatgacat aaaacgctgt tccctggcat atcctctatt tgcaaaacca
     3001 tatgccgagg gaaccggtaa aggtattact gcttcttcgg tcattcgtga tgcaggtgag
     3061 cttgaattaa cctgtacaca attgcttcaa aaatataagc aaccagtact ggtggaggag
     3121 tacctcccgg gaagagaatt tactgtaggg atagttggta gcggcaataa cgcaaaaact
     3181 attggaacta ttgaaatcat attactggat aatgccgaaa aagatgtgta ttcattcatc
     3241 aataaggaac aatgtgaaga acgggtagaa tacaggcttg cagatgatat tacagcacag
     3301 aagtcagcgg atgttgcact taaggcatgg agagcacttg gatgtcttga tgcaggccgt
     3361 gtggatatta aatgcgatag ctccggtaat ccacactttc ttgaagtaaa ccctcttgcc
     3421 gggcttcatc ccacacattc cgacctgcca ataatttgct ctaaagctgg catattgtat
     3481 cagcagttat tagcaaccat catgcataat gcgttaacac gttataacat gcttgataat
     3541 gcaccgcaaa aattatcttc attggctata aaatagtact tgcacaaata ctataacata
     3601 aaaaatttgt aataagtggt ttatacacac caaccctgaa tcaccggtgt gcaattgtaa
     3661 attccatttc aataacgttg gaatcatgga tataatcttt attcaccagt atgtacccga
     3721 tgacgctccg ctggatgaaa aggatgtgct ggagcagcga gatgaacttg ctgaaatttt
     3781 tattcagatg gggtacactg taggttcacg ggcaatacaa tcacatctgg acctgctatc
     3841 attgcaggaa ctttcgcgca gcagttgtat tgtatttaat ctgtgcgaaa gcctgtttgg
     3901 gcgtgatgac cttatgttcc ttgcaccttt tatgcttgag caatacggca ttccatttac
     3961 cggttcaggg tcacatgcca ttgcactaag ctgccacaag cttgaaagca aaaaactcat
     4021 gatgcagtat ggcattccca ccccggcata cattacaggc aataccgagt atcatgagac
     4081 cctgaatggc acctatatta tcaaaccata caacactcat gcatccatca atattaccca
     4141 ggattcaatt gtacagatac aatcccggag tgatttcaat gtactaaaag aaaagcttat
     4201 ggcttcaccc aatctttttg cagaagaata tattgacgga agggaattga atatttcact
     4261 tcttctcact gatgatggag tacagatact cccccatgcc gaaatacatt ttgataacta
     4321 tccgcccgat aaatataaaa ttgttgatta tgaagcaaaa tggattcata actcatttgc
     4381 gtatgaaaac accaaccgaa gcttttcctt taccgaagaa gacaggccaa tgttacatga
     4441 aattgacaga atatctttca aattatggga tattttcaaa ttaaatggct atgcacgtat
     4501 tgatttcagg gtaaaagata aagaagtcta tgtgcttgag gttaatgcta acccctgcat
     4561 atcaatttac agcggcttta tggctgcagt ggaggaatat ggattaacct ttgcacaggc
     4621 acttgacatt attattaaac atcctgttat tccaaaaaaa catctatgtg aagcggatga
     4681 agcatgtaat taaaaatctt atccagtaca gagatttccc tgtccaaacc gattgcgata
     4741 cagtagtatc aattcttgat gctaccggtt ttttttatcc agaagagata gatattgcct
     4801 tagaactatt aaacgaaaaa ataaattatc ctgatagctc aagttacgaa tttatttttg
     4861 ctgaatacca aacaactgta gttggatata tatgctatgg aaggatccca ttgacaaaag
     4921 cctcatggga tgtgtactgg attgcagtac atcctgattt ccagggcaac ggtattggat
     4981 acaccttgct ctcaaaagca gaagaaacca ttaaattaca taacggcaaa catttttata
     5041 tagaaacatc cggcagaaaa ctgtatgaac ccacccacac attctatctg aaagcaggat
     5101 acactgttag cgcagtattt aaggattttt atgctgacgg tgatgataaa ctggtatttt
     5161 ataaaaaatt agtataaaat ttacagcatt gtttggattt tgaaggcagt gaatctttct
     5221 atgtattctc atattgtaca gtaactatcg ccccaattaa tattttcttt gcttttttat
     5281 aatttccatt agaatcttga caaatagagt gttacattga tgtatattac tgtgaggtac
     5341 ctatggaatg ctcatgcggt ggtatattaa cggaaggacg aagctcgtat agtgttagtg
     5401 ggcagcattt ttatttagtc atagaagata tcccggccta taaatgcaac cggtgcggta
     5461 aaatattgtt tgaagatgat gtcgttgata aaataaaagc attactgaaa cgagttgaac
     5521 gggacacaaa agaaattgtt accggcacac cttcagtaca tacatacgac tattaatttt
     5581 tttacacata caggagaaca taaccatgcg cgtacgattt gcacccagcc caacaggctt
     5641 tttacatgta ggtaatgctc gaactgcagt attaaacttt ctgttcaaga aaaaatataa
     5701 tgccacatat attctcagaa ttgaagatac tgatatggaa cgcagtacca gggaatctga
     5761 agagtccatt ctcaatgact taaagtggct tggcatacag tgggatgaag gccctgattg
     5821 tggtggaacg tttggccctt acagacaatc tgaacgtttt gacatttacc gtgaatatac
     5881 tgagaagcta ctgcaaggcg gccatgcata tcattgctac tgcacccagg aagaacttga
     5941 tgcccagcgc cagaaggcag cacaggataa taaacctttt gtatatcctg gaacatgtag
     6001 agaccttacc cttgaacaaa aattggcttt tgaaaagcag ggcaggaaac ccacagtgcg
     6061 gttcaaggtt aaggatggga ttactctttc aatagatgac cagattaaag gcaacgttgt
     6121 cttcaacagt gaaaatattg gtggtgattt catcatagtc cgttctgatg gtgttcctgt
     6181 atacaattac attgtcatca ttgatgatac cttaatgaat gtgacccatg ttatccgcgg
     6241 tgaagaccat ttgcccaata caccaaagca gatgctcatt gccatggcgt tagggctccc
     6301 ggtacctgtt tatgcgcaca tgccactttt gcttggaccc gaccgcacaa aactatcaaa
     6361 gcgccatggg attacatctg ttgcagtgta ccgggaacag gggtatttac ctcaggcatt
     6421 attcaattat ttagccctac tgggttggac attggaatca ggtgaggaga tagttcctgt
     6481 tgaaaggctt gttgccgaat ttgagttatc aaagattgga aagagttccg ctatttttga
     6541 ttttcagaaa ctcaaatgga tgaattcagt atatttgcgt aatcttcctt ttgacaaagc
     6601 atttgagctg ttttatccct atattgaaaa agcatatcct actgtaagta acagcgaatg
     6661 gcttactcaa gtagttgata cggtacgggg caattgtgag atagcttcag atattgttaa
     6721 ggagatttct atcttctttg gcgacacagc tgaatttgaa gaagatgcca aaaacatgtt
     6781 agcaacaccg gaaggtaaga caatcattca gactgcatat gaacttattc actcaactat
     6841 caatgagagc aattacagta ctgaaacact caacactata aagcaaaaaa cagggctcaa
     6901 ggggaagatg ctttttatgc cgttcagagc aatggtaacc ggcagattac atggccctga
     6961 gctagataaa atactaccac tgttagggta tgaaaatttc acaaagcgta ttgaatattg
     7021 tcatcaacgc tttgttaaat aaattaaaca gagtaggtta cacaaagcac tcaaagtatg
     7081 aattttttat ttcaaaggcc atgttgaatc ataataaatg tattgacaat atctgtgcta
     7141 taaacaccca caatgataga aatgatattg agaatgacta tgcgtggcaa aaagatttct
     7201 caccccgaaa agtcggggtt cgaaatgaca agttaagatt tctcatcctg atagataggg
     7261 ctgatataat taaatcgtat tttccagggg aatatatgca attcatgaaa aagttaggat
     7321 ctacaataat tgtttttacg cttctaaccg tttgtgcgtg ttctgcacat gcacccggta
     7381 caatccctgt aggcacatgg cagtacgatc ttcttgtcaa cggcgttgaa atggggaagg
     7441 ccactatcca gaacagcatt gaagacaacc agtatgtata tcgctttgat ttgacaatag
     7501 gaaatggtac cacacggtca caggatacta tagttgaaac attagagtgc aaaccaataa
     7561 aacttgaaaa acatacctat ataaaacaaa caaccactga acaaaaaatg gatattattg
     7621 cacagtgtaa tggccgcacc attatcttaa tagaaaataa tatgaagagt atcataacat
     7681 tggaccagga ttatgttctt gatggtaatt attttctgta tatgttaata cagaataaat
     7741 tcaaaaaagg tttacagatc acacataacg tctatgaccc atcaatagaa cgagaagcag
     7801 caattcctgt aaccataaaa cttataggaa ctgaaacaat ttcagttaac agtattctgt
     7861 ataaagcctg gaaaattgag cagagcattg gtaaaattaa aaatattatt gtatatcttg
     7921 atgaacatgg cgttgtgtta aaaagttcta taaaaatgtt aaatatgcag cttgatatca
     7981 tccgtaagga ctgatcatga aaaaaatata tgctattgtt atatctgcca ttgttggtgg
     8041 atacttacta tacctgtatt cgcaaccatt gcctgccagt ctttcaacag ttcccattgc
     8101 caatataacc agtaaattat gggaagagaa tatatccctt ttcatggtat catccgatga
     8161 agcattttcc ataacccaga caccatttca aaaaattctt tcgtcacgga acaaaaatac
     8221 ttcgtatgca tacatcattg catcaggaaa aaataaagag tatcatcaaa atcctgtaga
     8281 aaaagattta aaagacacgc gattattgca aatacataac ccagtaattg tacagcatgc
     8341 aaaaacaatt gcctcaagct ccaatcccat tgaagaagct gaagagttag tttataacac
     8401 aatagaaacc aaaactgaag ggattcccat catacctgca cctgcaattt ttgaattaaa
     8461 aaaaggtgac tgtaccgagc atgcaatagc aacaattgca ctgctgaggg cactgcacat
     8521 tccagcacga gcagtggttg gcatgatagc agcaaaagaa tatgcaggca aaaaaaatgt
     8581 atttgtgttt cacatgtggg ctgaagcctt ttataacaac gcgtggctgc ttgttgatgc
     8641 cactcgtccg ggaaaaaaga aaacaaacat ctacattgca ttgacatatc attcacttga
     8701 aacggaaatg cctctagaat ttttagaagc catggccaac attcagaatc taaaagttac
     8761 atatctttct gaatagcatc taacaatata cgtaatgtca taccagggtt aataagttta
     8821 catagtatgg catatagcac cacacccagt gtaacaagca ccactgtata tgtaagacct
     8881 ttctcatacg gtaaaaacga ttgcaaaata tacagtgaaa ttcccataat acctgaagca
     8941 atgaaatact gcagcagagt aacagtaaac cttttatcca ctttcacttt tattgaatgc
     9001 aaaagtttta tgacaagagc tatcgccaca caatataatg aaatgctgtt agcaagtgcc
     9061 agcccaccat gcaaaaggtg cgtacgcata agcacaaagc caagcactgc attcaggata
     9121 attgcaactg ttgaaattac attaataaat acggacgacc tcatactgat aaagagcggc
     9181 atgagaatcc gcaccacagc aaatgcagga ataccaaacg ctgcacatgc aagtgcctta
     9241 taggtcatgg ctgtttcaaa actatagaaa tttccatgct caaagaatgt agccagtata
     9301 accttccccg ttgcaataag cccagcactg gcaggaagtg caataaatag tgatgcttgc
     9361 agcattgatg tcattgttgc ctgcagacgc gcaacatccc tgccagccca caaccgtgaa
     9421 acctcaggat gaaatgcatt gctcattgaa atgataaaaa ctccacttac aatctcaatg
     9481 aatctatcag cataatacag gtagctaaca ctaccctgct tgagcgttga tgccatcatg
     9541 gtgttgacta gcacattgat atgatacaca gcaacaccca tcactgaggc tacaatcata
     9601 cgcagcactt tagtgtgtcc tgactgtata tgacccaatg tcaggtgcac acctgtttta
     9661 tacaatggaa tgaaaacaac cacgcattgc aaaatgccac cggctacaac acccacccca
     9721 agccatataa catttgaata cagatatgca ataaagcaaa aaagaataat gcccacgtta
     9781 agcaacagt
//
LOCUS       DAOSZP010000005         9766 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_5932_length_9766_cov_4.008959, whole genome shotgun sequence.
ACCESSION   DAOSZP010000005 DAOSZP010000000
VERSION     DAOSZP010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 9766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9766
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5932_length_9766_cov_4.008959"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(283..663)
                     /locus_tag="PK348_00265"
     CDS             complement(283..663)
                     /locus_tag="PK348_00265"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HXK64678.1"
                     /translation="MIYYIDTNAILRYLLKDNIEQFNKANVIFEKAREGDLYIVVLES
                     VLVECIYVLMKFYKVPKNEIVSKLETLLNYKGVKNSDKTELLKALQIFKEKNIAIVDC
                     IVYSKAKQSQSNIFTFDEKLLKLK"
     gene            complement(660..887)
                     /locus_tag="PK348_00270"
     CDS             complement(660..887)
                     /locus_tag="PK348_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008287623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HXK64679.1"
                     /translation="MKTIAKITRKGQVTIPKKIREMLGSDIIEFKVHRNDIIIKPVKS
                     IGGSLHKYSKRYVPFEDIRDTVWDEVVRKNK"
     gene            complement(973..3825)
                     /locus_tag="PK348_00275"
     CDS             complement(973..3825)
                     /locus_tag="PK348_00275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HXK64680.1"
                     /translation="MSTILVIDDKKDNLVSITALLKNLIANCEVVTAQSGKEGIEKAE
                     KHNPDVIILDIKMPEMDGYEVCQILKNNPKTRHIPVIFLTAIHTDTQSKVKGLKLGAD
                     AFLTKPVDQAELVAQINAMIRIKHAEDMLRKEKDLLEDIVLQRTSELLASQMRYKSLF
                     NSISDMVIIHRRDGKILEVNNTTCDRLGFSHDAMMLMTLQQLTAEGDSDRINSYLQSI
                     NETADPYETSFVDRNGTTIPVEIVSTIIYYLNENAILSVARDITERKHIEHVRNQLSI
                     VLDKSDDAIIGATLDGKIASWNKAAQHIFGYAFDEVVGCDLAILSPPYRPDEFDQLLL
                     IIKNNEAIDHYETEGLDKKGAIHNLSMTISPLVDEHDSIIGCSIIARDFTDVKRTQEK
                     LKKGLDIIQNLEDIAPAYFITLNEEGNILTCNKAMLQATGYSLSQVQGKTCCDIFIPD
                     VGQKSFRNQLSTMKRTRATRIFESPINTKSGEEKIVQWHARPVYSDTEFDYFMFIGID
                     ITEKKQLEKKILQANLEERNRIARDLHDGLGQHLAGIIFKTEMLRLKIKNDYPQEANE
                     IVEIQNMVHQAIEQTRLIARGLSPVDLSTKGLYSSLQDMIADVNKNFAIKTILEWNIT
                     SNIDEAIDTVHVYYIIREALNNAVKHASPKNIAITISEDNAYYYVKIMDDGKGIPDEI
                     DLAKGMGMHIMQYRAWIINASFQVQNNKSGGTLVQCIIQKSTQKAVIDKPKTGKKYTI
                     VIVDDHPIVRQGLQQLINTQKNLEVIGQATSADEAIDVVNRKIPDCIIVDISLNGTSG
                     IELVKALKQRFPKLPALVLSMYDEKLYAERAIKAGARGYLMKQEAPDKIIQAIYTVLK
                     GDVYLSSEMKEQMVQSLSDKGTMDSSVEALTNREFEIFQLIGHGLGNKHIAQKLHISV
                     KTVENVREKIKMKLHLETSQDLLQYAIEWVRENSGN"
     gene            complement(3822..4877)
                     /locus_tag="PK348_00280"
     CDS             complement(3822..4877)
                     /locus_tag="PK348_00280"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXK64681.1"
                     /translation="MNKEFLHSISHELKSPLNSILTLSKVLLMQADSRLTLEEKEYLE
                     IIERNGQKLLALINDLIEVFEIDSGIQLSVTDVSLSSVLHNVCERYEGIMRKKGLQLH
                     MHCDDIMLNTDEARLYQIFENLIDNAVKFTEEGSITIAAYKKDGHCVVSITDTGIGIR
                     SEYCDALFSGFTRASGSLSSKYPGTGLGLTIVARLVALLGGTVAVDSQLGKGSTFTVR
                     LPLQKEHEDLQQQRKTILVIDDDPDSIVVVKALLSKLYDIVETHSGQEGLDLAQDLKP
                     ACILLDLSLPDLHGLQVVKQLKENEQTSHIPVIIVSASSLKDERRTIMASGCDDFIQK
                     PFAIEEFVLKVQRWVTQ"
     gene            complement(4870..5367)
                     /locus_tag="PK348_00285"
     CDS             complement(4870..5367)
                     /locus_tag="PK348_00285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK64682.1"
                     /translation="MAKPAEYFIKSKNLDEFRNDIIACDGNFDFDIEEMIALGSAYLE
                     RFPDCFSNRSCQDVQLGYQLARICLVEKLIIDFPSDVKDAFRKMFFSAQAVNEQMEYL
                     KQKLTYDELSNFVDTIQKRLEEYHLKVVSLPKGMIKERFVGGITNLFNIAYLLKMNVA
                     QKAHE"
     gene            complement(5502..6470)
                     /locus_tag="PK348_00290"
     CDS             complement(5502..6470)
                     /locus_tag="PK348_00290"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HXK64683.1"
                     /translation="MRKGISIVLGLLCLLSCAKDITKVTIAKTPVPKNFTATDIKVIS
                     YNGNDVSCMLFAHRFSQGNLVYFEIVPAFDVEKITVMFNEKGIPTTKRSWGFRGFFAI
                     APDQKPGTHNFTVTIGTKKIDIPVVVVRTPFSVSTIPMDLGKFSKKEYLESPEVLEFI
                     RQCTEKKQKAFATHYNDLIRGRAFHPRDVHEVNSPFWAKRVYKLHDSISGKTQTVERI
                     HRGIDLNGNTGDPVYAMLDGIVVLAEMLFYEGNMVIINHGNGMFSYYMHMDKLYVASG
                     DTVVAGQLIGAVGSTGMSTGPHLHVSLVVDGVQVNPMSVVMLPIRD"
     gene            6810..7163
                     /locus_tag="PK348_00295"
     CDS             6810..7163
                     /locus_tag="PK348_00295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK64684.1"
                     /translation="MSTQVSFSKLEKEFLPQFREKISTSEDIIDVQKYFSYAIKEMLQ
                     KAFEKEEITITEEDIELTTIPPYYTIKKIDSSIQSLWEHSDLKDILQRFAEVAHKRYL
                     HLQKNPSKTNKKIRR"
     gene            complement(7204..7572)
                     /locus_tag="PK348_00300"
     CDS             complement(7204..7572)
                     /locus_tag="PK348_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HXK64685.1"
                     /translation="MYIFELIAEQRIREAMERGEFDNLPGMGKPLPKDELDNVPPELR
                     MAYKILKNAGIVPEEVELRKEIYSLQQLIDACYDDEEMKGLVKQLSAKQLRYNILMEQ
                     KGIHVTDYEYRKKIMEKLSK"
     gene            complement(7650..7976)
                     /locus_tag="PK348_00305"
     CDS             complement(7650..7976)
                     /locus_tag="PK348_00305"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HXK64686.1"
                     /translation="MTIDYQKIEEAAKLLELGDRASLQQVKHAYRKLMQKWHPDHCKE
                     DKKLCDEMTKKLTEAYKLLIEFCYSYRIPLTEQKLVEELGNEDPEAFWKRKFGSDPHW
                     GGPGYR"
     gene            8136..>9766
                     /locus_tag="PK348_00310"
     CDS             8136..>9766
                     /locus_tag="PK348_00310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK64687.1"
                     /translation="MKLSPIIMTFLIIPHILFAENYWQRYADSLIKEQKQAKEQPYIA
                     PFVIEYLDTRIAMAQELSRSFDSTLQNNNEHQEHITNCCKQALSLCFDKTLIDITKEQ
                     VTKELQHSDISNNDAIIIHTELILSSIFRQFDTIFTNNSTQQYDIKQIIQRCQPPFDD
                     LSQSTRFQELSAHANFITQQQHFITSIASLCNTTQYDDEQIRQNPEHAKALLAQLENA
                     LINVPEKTATYQQTDGIPYQITIPQQLDCARAIYEIQNKRDAIVTEKDATKIDDIESI
                     ARSYITPIENQIAEQKKLLVIMKSTDGVAVENEEAFNNVVHRFETQSKLLHDYANATT
                     LYCQLSLLQAPHINYSSRCQHIVYVASHIQKLVQSLGDSSKEIIPEVKQLFEVLKAFL
                     YTDAPKENNDERATTLQTLHTIKENIYTIASTRKDDTPNPALCNLEIAMNIETLEKGI
                     KLFNTQTYAKQALMRYASTIQKAFELVQTGFSDNTIQQIIEMQSAIPVVDNFDVQQII
                     NEYTSQQYVLRKLRADSASLMQRIESYKKKGIRIN"
BASE COUNT         2942 a         2106 c         1591 g         3127 t
ORIGIN      
        1 ctggatgtac ccgcgatggg aaaactccct gtacagatgt gccatctact ttgagatagt
       61 ggccacatcc tgttccacaa aaggtgcata cacctggcat ataactcata gattatcctc
      121 actaaccata atgaaattca aaatcataca gttctgtcct aagaaaatgc ttccttattt
      181 aattgctata gtgaatatgg cacattgtca ggatgttgta aatagggaat tttcttattt
      241 ttgttatagg ggaatttact tatgcagcaa tgattgaatt gtttatttta attttaataa
      301 tttttcgtca aacgtaaaga tgtttgattg agattgtttt gctttactat atacaataca
      361 atctacaata gcaatatttt tttctttgaa tatctgcaaa gcttttaaca actctgtttt
      421 atcagaattt tttacacctt tataattcaa tagtgtttct aatttactaa cgatttcatt
      481 ttttggaact ttataaaatt tcattaatac ataaatacat tctaccaaaa cactttccag
      541 aacaacaatg tataggtcac cctcccttgc tttttcaaat atgacatttg ccttattaaa
      601 ttgctcaata ttatccttta acaaataacg taaaattgca ttggtgtcaa tatagtatat
      661 catttgtttt ttctaacgac ctcatcccat acggtgtctc gtatatcttc aaatggcaca
      721 tagcgtttag aatatttgtg tagagaacct ccaatacttt taacaggttt gatgattata
      781 tcattcctgt gtaccttaaa ctcaataata tctgaaccga gcatttccct tattttttta
      841 ggaatggtaa cctgcccctt tcttgtaatt tttgcaatag tcttcatatg ttccttacat
      901 taataatttt cattacttta catcaaaagt aatgaattat aaagaatttg tcaaataatt
      961 cttttacttt tcctaatttc ccgaattttc tctcacccac tcaatcgcat actgcaaaag
     1021 atcctgggat gtttccaggt gcagtttcat cttaatcttt tcccgtacat tttcaacagt
     1081 ctttacactg atatgtaatt tctgggcaat gtgtttgtta cccaagccat gccctattaa
     1141 ctgaaatatc tcaaattcac ggtttgtcag tgcttctaca gagctgtcca ttgtaccctt
     1201 atctgataaa gattgcacca tctgttcctt catttcactg gatagataca cgtccccttt
     1261 taagactgta tagatggcct gtataatctt atccggtgct tcctgcttca tgagatatcc
     1321 ccgggcacca gctttaatag cccgctcagc gtataacttt tcatcataca tggaaagcac
     1381 aagtgccggc agtttaggga aacgctgttt taatgcttta accagttcaa tgccacttgt
     1441 cccattcaat gaaatatcca ctataatgca atcagggatt tttcggttaa ctacatctat
     1501 tgcctcatcc gctgatgttg cctggcctat cacctccaga tttttctgag tattgataag
     1561 ctgttgtaaa ccctgccgca caataggatg atcatcaaca ataacaatcg tatacttttt
     1621 gcctgtcttt ggtttgtcta taacagcctt ttgagtgctc ttctgaataa tacattgcac
     1681 cagcgttccg ccgcttttgt tgttctgcac ctgaaacgac gcattaatga tccatgccct
     1741 gtactgcatg atatgcatac ccattccttt tgccagatca atttcatcag gtataccttt
     1801 gccatcatcc attatcttta cataataata tgcattatct tcagatattg taatagcaat
     1861 gttttttgga ctggcatgct ttacagcatt gttcaatgct tcacggataa tgtaatatac
     1921 atgtactgta tctattgctt cgtcaatgtt acttgttata ttccattcca ggatggtttt
     1981 gattgcgaaa tttttattta catcagcaat catatcctgc agtgatgaat acagtccctt
     2041 ggtgcttaaa tccaccgggc ttaaacctcg tgcaatgagc cgtgtctgct caatagcttg
     2101 atgcaccatg ttctgtatct caacaatttc attagcttcc tgaggataat cattttttat
     2161 tttaagcctc agcatctcag ttttaaatat tatccctgca agatgctgcc ccaggccatc
     2221 atgtaaatcg cgtgctatgc ggtttctttc ctctaagttt gcctgaagta tctttttttc
     2281 aagctgcttt ttctccgtta tatctattcc aatgaacatg aaataatcaa attcagtatc
     2341 actgtatact ggccgtgcat gccactgaac aattttttct tcaccacttt ttgtattgat
     2401 ggggctttca aaaatgcgtg ttgctctggt cctcttcatt gttgacaatt gattgcggaa
     2461 tgacttttgc ccaacatccg gaataaaaat gtcacagcac gttttgccct gcacctgact
     2521 cagtgaatag cctgtagcct gcaacatggc tttattgcag gtgaggatgt ttccttcttc
     2581 atttaaagtg ataaaatatg ctggagcaat atcctctaaa ttctggatga tatccagccc
     2641 ttttttcagt ttttcttgtg tgcgttttac atcggtaaag tcacgtgcaa ttattgaaca
     2701 gccaataata ctgtcgtgtt catcaacaag gggggagata gtcatagaaa gattgtgtat
     2761 tgcacccttc ttatccagcc cctctgtttc ataatgatct atcgcctcat tgttcttgat
     2821 tattaataac aattgatcaa attcatcagg cctgtatggt ggcgatagta tagctaaatc
     2881 acatcccacc acctcatcaa atgcgtagcc aaaaatatgc tgcgcagctt tgttccagct
     2941 ggctattttg ccatctagtg tagcaccaat gattgcatca tcagacttat caagcacaat
     3001 tgataattga ttacggacat gttcaatgtg ctttcgttca gtaatatcac gcgccacact
     3061 caagatagca ttttcattta ggtaatagat aatagtgctc acaatttcca cagggatggt
     3121 agtgccgttt ctgtcaacaa atgaggtttc atagggatct gctgtttcat taattgattg
     3181 caagtatgaa ttaattctat cgctatcgcc ttcagctgtt aactgctgca gggtcataag
     3241 catcatggca tcatgtgaaa agcccaaacg atcacaggtg gtattgttta cctccaatat
     3301 ttttccatcc cttcggtgaa tgataaccat atcagatata ctgttaaaca gtgacttgta
     3361 gcgcatctgc gaagctaaaa gttcactggt gcgctgtaac actatatcct caagcaggtc
     3421 tttttctttt ctaagcatat cctcagcatg ttttatgcgt atcattgcat taatctgcgc
     3481 aaccagttca gcctgatcaa caggcttggt taaaaatgca tctgccccta attttaaacc
     3541 tttgaccttg ctctgcgtat cagtatggat agcggttaaa aagattacag gaatgtggcg
     3601 cgtctttgga ttatttttta aaatctggca tacctcatac ccatccatct cgggcatctt
     3661 tatatcaagg ataataacat caggattgtg cttctctgct ttttctatac cttcctttcc
     3721 ggattgtgct gtgactacct cacaattggc aatcaggttc tttagaagtg cggtaatact
     3781 taccagatta tcttttttat catctatgac caaaattgtg ctcattgtgt aacccagcgc
     3841 tgaactttta gcacaaattc ttcaatggca aaaggtttct gaataaaatc atcacacccg
     3901 gatgccataa tggttcttct ttcatctttt aatgaagaag ctgatacaat aatcaccggt
     3961 atatggctgg tttgttcatt ttcttttagt tgttttacca cctgcaaccc gtgcagatca
     4021 ggcaatgata aatcaagcaa tatacatgct ggttttaaat cctgtgcaag gtccaatcct
     4081 tcttgcccgc tgtgagtttc aactatgtcg tacaatttag acaaaagggc cttaaccacc
     4141 actatgctat caggatcatc atcaataaca agaatagttt tacgttgctg ctgcaaatct
     4201 tcatgttcct tttgtaacgg taaccgtaca gtaaatgttg accccttgcc taactggcta
     4261 tcaacagcta ctgtaccccc taacagtgca acaagccttg caacaatggt aagaccaaga
     4321 ccggttccag gatattttga cgacagtgac cccgaagccc tggtaaatcc tgaaaaaagc
     4381 gcatcacagt attcactgcg gatcccaatg ccggtatcag taatactgac cacacagtgc
     4441 ccatcttttt tgtatgctgc tattgttata cttccttctt ctgtgaactt aactgcatta
     4501 tcaataagat tttcaaatat ctgatacagg cgggcttcat cagtattaag cataatatca
     4561 tcacagtgca tgtgcaattg tagccccttt ttgcgcatga ttccttcata gcgttcacac
     4621 acattatgca atactgaaga taacgataca tccgtcactg acaattgtat accactatca
     4681 atttcaaaca cttcaataag gtcattaata agagccagaa gtttttgacc attgcgttca
     4741 ataatttcta aatactcttt ttcttcaagg gtgaggcgac tgtcagcctg cataagtaac
     4801 acttttgata gcgttaaaat ggagtttaac ggtgatttca attcatggct tatgctgtgt
     4861 aaaaactctt tattcatgag ccttttgcgc tacattcatt tttaataaat acgcaatatt
     4921 aaaaagattg gtaattccac ctacaaagcg ctccttgatc atgccttttg gcaaactaac
     4981 aactttaagg tgatactcct ccagacgctt ctgtattgtg tcaacaaagt ttgatagctc
     5041 atcataggtt aatttttgct ttaaatactc catttgctca ttcacagcct gagcgctgaa
     5101 aaacatcttt ctgaaagcat ctttaacatc agaagggaaa tctataatca atttttctac
     5161 taaacagatt cttgctaatt gatatcccag ctggacatcc tggcagcttc tatttgaaaa
     5221 gcaatcaggg aaacgttcca gatacgcgct tcctaatgca atcatctctt caatatcaaa
     5281 atcaaagttg ccatcacagg caatgatgtc gtttctgaat tcgtctaaat tctttgattt
     5341 gataaaatat tcagctggtt ttgccataca cttattccag tttattggca gtaactatcg
     5401 cataaaaact atagcacatc attactattt tatatagtta aaatagtata ccaattttgt
     5461 aaagaagatt atggaagaga atgtatacaa atacggtttg gttaatcccg tattggcagc
     5521 atcacaacac tcatgggatt tacctgcact ccatcaacca caagtgacac atgcagatgt
     5581 ggacctgttg acatgccggt gcttcccaca gcacctataa gctgtcctgc cacaaccgta
     5641 tccccgcttg caacatacag cttatccata tgcatatagt agctgaacat gccattgccg
     5701 tgatttataa taaccatatt gccttcataa aagagcattt cagcaagcac cacaatgcca
     5761 tcaagcattg catatacggg atccccggta ttgccgttta aatcaatgcc acggtgaatc
     5821 cgttcaactg tttgtgtttt tcctgaaatg ctatcatgaa gtttataaac tctttttgcc
     5881 cagaacggcg aattaacctc atgcacatca cggggatgaa acgctctgcc acggataaga
     5941 tcattataat gtgtggcaaa tgccttttgt tttttttcag tacactggcg tataaactcc
     6001 aaaacttcag gtgactccag atattctttt ttagaaaact tgcccaaatc cataggtatg
     6061 gtggaaactg aaaatggtgt tcttacaacc actacaggta tatcaatttt ttttgtaccg
     6121 atagttactg taaaattatg tgtcccaggc ttttggtcag gtgcaatggc aaaaaatcct
     6181 ctgaatcccc agcttcgttt tgtagtggga ataccttttt cattaaacat tacagtaatt
     6241 ttttcaacat caaaagcagg aactatctca aagtatacaa gattaccctg tgaaaagcgg
     6301 tgtgcaaaaa gcatgcagga aacatcattg ccattgtagc ttattacctt tatatcagta
     6361 gcggtaaaat tttttggcac cggtgtttta gcgatagtta cttttgtaat atcctttgca
     6421 catgaaagca aacatagtaa accaagtact atacttattc cttttctcat tccagaaacc
     6481 tttttatcct tatgtgcgct tctcccgcgt aatacatttt ttacctcacg cagtgtttgt
     6541 tgaatgctca gatatttggt tattacaggc tttggcctca attactaatc attacgcagg
     6601 tgctcaagcg cttgagcgac tgcgattatt gatttatata tttcttttat tcagacgtca
     6661 acaaaagaaa gtgccaaagt acaatgcttt tagattttcc ctgcgtgatg tattaattta
     6721 tctctcaatt acgaagttga tgcttgattt ttattcaatt cctgtatata ctatagtata
     6781 tacacaaact ataccacaag gaggtttgta tgtccactca ggtttccttc agtaaattag
     6841 aaaaagaatt cctcccccag ttcagagaaa aaataagcac ctcagaagac atcattgacg
     6901 tacaaaaata tttttcatat gccataaaag aaatgctgca aaaagccttt gaaaaagaag
     6961 aaataactat taccgaagaa gatattgagc ttaccactat accaccatac tatacaataa
     7021 aaaaaataga ttcatccata caatcattat gggaacattc cgatttaaag gacatactcc
     7081 aacgatttgc cgaggtagct cacaagcgct atcttcattt gcaaaagaac ccatcaaaaa
     7141 ctaacaagaa gatacgacga taagcaatta tacacctgca gcctaagcgg ctgcgtagca
     7201 ttgttacttt gataattttt ccatgatttt ttttctatat tcgtaatcag tgacatggat
     7261 gcctttttgt tccatgagta tgttatagcg tagttgtttt gcactgagct gctttacaag
     7321 tcctttcatc tcttcgtcat catagcaggc atctataagc tgttgcaatg aataaatctc
     7381 tttgcgcaac tccacctctt caggaactat cccggcattt tttaaaattt tgtatgccat
     7441 acgtagttct ggcggcacat tgtccagttc atctttgggc aatggtttac ccatccccgg
     7501 caggttgtca aactctcccc gctccatggc ctcacgtata cgttgctcag ctataagctc
     7561 aaaaatatac ataccttact catcttaagt attcaggcta agagtatata acaaacatac
     7621 ataatatggc ttccgttgta aaatattttt tatctatacc cgggacctcc ccagtggggg
     7681 tcagagccaa attttctttt ccagaacgct tcagggtctt cattccccag ttcttcaaca
     7741 agcttttgtt ccgtcaatgg tattctgtaa ctataacaaa actcaataag cagcttatag
     7801 gcttcggtga gctttttcgt catttcatca cacagctttt tatcttcttt acagtggtcg
     7861 ggatgccact tttgcattaa tttcctgtag gcatgtttaa cctgctgtag ggaagcccta
     7921 tcgcccaatt ccagaagctt tgcagcttct tctatctttt gatagtcaat agtcatatat
     7981 cactcctgaa taataccatt accacattac aaaggtagca tagaaaaagc ataatttcca
     8041 tcttgccgtt tgcaggtaca cgtatcgttt accgtaaata cagtaataaa tcatggtatt
     8101 gcgttcgata attaattgaa gggagaacta ttgccatgaa actatcacca attataatga
     8161 catttcttat tataccacac atactctttg ctgaaaacta ctggcagcga tatgccgatt
     8221 cactcataaa agaacaaaag caggcaaaag agcagcccta cattgcacca tttgtgattg
     8281 agtatcttga cacccgcatt gccatggcac aagagttatc acgttctttt gactcaacac
     8341 tacaaaacaa caacgaacat caggaacaca taaccaattg ctgtaagcag gcactatcgc
     8401 tttgctttga taaaacactc attgatatta caaaagagca ggttaccaaa gaattacagc
     8461 attctgacat aagcaacaat gatgctatca tcattcacac tgaattgata ctttcttcaa
     8521 ttttcagaca gttcgataca atctttacta acaatagtac acaacaatat gatataaaac
     8581 aaatcataca acgatgccag ccaccgtttg atgacctttc acaaagcaca cgctttcagg
     8641 agctttccgc acatgccaat tttattacac agcagcaaca cttcattacc tctatagctt
     8701 cattgtgcaa caccacacag tatgatgatg aacaaatacg gcaaaaccca gaacatgcta
     8761 aagccctgct tgcacaatta gaaaatgcac tcattaatgt tccagaaaaa acagccacat
     8821 accagcaaac tgatggcatc ccctatcaaa taaccatacc acagcaactg gattgtgccc
     8881 gtgcaattta tgaaatacag aacaaaaggg atgcgatagt aactgagaaa gatgctacaa
     8941 agattgatga cattgaatcc atagcacgtt catacataac acccatagaa aaccagattg
     9001 ccgaacaaaa gaagcttctt gtaatcatga aatccacaga tggcgtagcc gttgaaaatg
     9061 aagaagcatt taataatgtt gtacatcgtt ttgaaacaca atcaaagctt ttgcatgatt
     9121 atgcaaacgc aacaacgctg tactgccagc tatctctgtt acaggcaccc cacattaact
     9181 attcaagtcg ttgtcaacac attgtatatg ttgcatcaca cattcagaaa ctggtacaat
     9241 cgttaggcga tagttcaaaa gaaataatac ctgaagttaa gcaactattt gaagtactga
     9301 aagcttttct ttatactgat gcaccaaaag aaaacaatga tgaacgtgcc acaacgttgc
     9361 aaacactgca cacaattaaa gaaaacatat acaccattgc cagcaccaga aaagatgata
     9421 cacctaaccc tgcactgtgt aacctagaaa ttgcaatgaa cattgaaaca cttgaaaaag
     9481 ggataaaact ctttaatacg caaacatacg caaagcaggc acttatgcgc tatgcgtcta
     9541 caattcagaa agcctttgaa ttggtgcaaa ctggttttag cgataacaca atccagcaga
     9601 ttattgaaat gcagtcagcc atacctgtgg ttgacaattt tgatgtacag caaataataa
     9661 atgaatatac cagccagcaa tatgtgttgc gtaaactacg ggctgacagt gcatcactta
     9721 tgcagcgcat agaatcctac aaaaagaaag gcatcagaat aaacga
//
LOCUS       DAOSZP010000006         9506 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_6158_length_9506_cov_3.227912, whole genome shotgun sequence.
ACCESSION   DAOSZP010000006 DAOSZP010000000
VERSION     DAOSZP010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 9506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9506
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6158_length_9506_cov_3.227912"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..800)
                     /gene="aroF"
                     /locus_tag="PK348_00315"
     CDS             complement(<1..800)
                     /gene="aroF"
                     /locus_tag="PK348_00315"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HXK64688.1"
                     /translation="MIIVLKPNVTQDEIDHIVGKIKSLNLQPHVSKGQYRTIITVIGD
                     EDIIREQPLEAISGVESVKPILKPYKLASKETHPDRTIIRVGDIDIGGDKIVLVAGPC
                     AVEGEKEIIEHAKMVKQAGASMLRAGAYKPRSSPYTFQGYGALGLKYLAKAKEETGLP
                     IITEVMDPRDVELVAQYADVLQVGTRNMQNFNLLKELGKINKPILLKRGMSATINEFL
                     MSAEYILSQGNKQVILCPRGIRTFEDITRNTLDAGAVPVLKDFSHLPVV"
     gene            complement(832..1878)
                     /gene="pheA"
                     /locus_tag="PK348_00320"
     CDS             complement(832..1878)
                     /gene="pheA"
                     /locus_tag="PK348_00320"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: protein motif:HMM:NF008865.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HXK64689.1"
                     /translation="MDTASFEKNIIESLSQLTELYSKELQNIMKEGEQGIFPVKAKII
                     SHLQQYARPPLTPELIEKIATEIFSVALSAARPLKVAYLGPVGTFTHIALLEIFGDVI
                     EAVPHRTISDVFDEVETGRATFGVVPIENSTEGAVTYTMDEFLDTDLKIVSEKSLRIT
                     YSLLSVCDSIEKIKKIYSHPQSLAQCKNWLKANLPNVEINQVNSTAKAAETASWDKYS
                     AAIASNISAKIYNLNIIADGIEDSRQNYTRFLVLGKHDNPPTGNDKTSIVCAVKDQPG
                     ALYKLLKPFSEHGINMTMIESRPDKKKMWAYNFFIDFNGHRTDPVVQKALEAMKQETI
                     FLKVLGSYQAEKHD"
     gene            complement(1880..2419)
                     /gene="scpB"
                     /locus_tag="PK348_00325"
     CDS             complement(1880..2419)
                     /gene="scpB"
                     /locus_tag="PK348_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008594845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HXK64690.1"
                     /translation="MGDSDDLFGIVEAILFVSAEPVPLQFFTRQLGIDATQAKIIIDS
                     LIDEYEDRDKGIKIIEISGGYQFVTNPKFASQLRQVMGYKKKETLSKGMLETLAIIAY
                     KQPIMLAEIEELRGVSSRMMVAALMRKNLIKPVGRKDVPGRPLLYGTTDEFLRYFGLN
                     KLSDLPKLSEIKEFNFEEE"
     gene            complement(2537..3286)
                     /locus_tag="PK348_00330"
     CDS             complement(2537..3286)
                     /locus_tag="PK348_00330"
                     /inference="COORDINATES: protein motif:HMM:NF014654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HXK64691.1"
                     /translation="MITPDNNDVQCILHIENFDGPLDLLWDLIKKSKIEITQVSIADI
                     TEQYLEYLKLMESINIPVAMEFIVMASELLYYKSKALLPGADIGDEFFVPPLPPELIQ
                     KLLEYKKYQIASKNLLKLHEMQSDVFTRQIPPLSFEEDVYIEVNLFDLLNAFVDVLEA
                     EKETEQQRIVFDEILVSDRIMLLVSMLQDKDKIEFHDMFTQNPGRMEVVVTLLALLEM
                     TRIGAIAVLQHAQFGNIYIFKKDLNKVDTLH"
     gene            complement(3442..3801)
                     /locus_tag="PK348_00335"
     CDS             complement(3442..3801)
                     /locus_tag="PK348_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXK64692.1"
                     /translation="MAKILIVDDAKFMRTLVKDALVPKGHEIIGEAENGLEAVQLYKK
                     LRPDLVTMDITMREKDGIEAAEEILQFDPNARIIMVTALGQENLLTRAIKIGVKDFVV
                     KPFPPERLQKAAEKALA"
     gene            complement(4021..5067)
                     /locus_tag="PK348_00340"
     CDS             complement(4021..5067)
                     /locus_tag="PK348_00340"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HXK64693.1"
                     /translation="MSEKIKVLVVDDSALVRKVISDILESDKEIEVIGTANNGKSAVF
                     KNLTLNPDVITMDIEMPIMNGLEALQEIIKTNPKPVIMMSVLTQYGAEATFKALEYGA
                     VDFIPKPSTVLAMSMDDIAELLITKVKAVAKASLNLHVTKSEHVSETNKVSIIARHED
                     KKKIVGIGTSTGGPSALQRVLQKLPKNFPSPILVVQHMPPGFTKAFAERLDSLCNVSV
                     KEAEDGEKVLNGCVYIAPGDAHIVVEKTGDTRVIRLSQAEKVSGHRPSADVLFHSLAK
                     EDSHNVIGVIMTGMGRDGARGIKEIHDNGGYTIAQDESTSVVYGMNRVAVEMGGVDAV
                     VDVDSIPEEIISHI"
     gene            complement(5073..8138)
                     /locus_tag="PK348_00345"
     CDS             complement(5073..8138)
                     /locus_tag="PK348_00345"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HXK64694.1"
                     /translation="MGFSLGEYQDMFLEEADELLQELNQNLLKLEKNPSDDEIINNIF
                     RAAHSLKSSAAFVGLTDLGDLAHKMENLLQGIRDKVMKVTPAIVDVLFQCFDVINDVI
                     REVSSGNPPSKDFSSLIKKIESVGKQGNIQEEKKEAPAPKRIQLSTNIIKDIRDGINN
                     GLSCCEIAITLDPDAAMKSLKVQLVLTNLEKIADILITIPSIEEVSMPEFDNRVQIIL
                     LTSATVEELKKACEVDQVTRIDLRKIELVKKDNKVALKYHEQETLFEEKPEADTKARD
                     VEEDVIPDEEEKDEIIIRSKDDKKTLQLRTVKVSVEKLDQLLNNVGELVIANSGFYKL
                     YEEMRRLNIEKSIINEFKNRMEQMSRIAKDLQSGIMKTRMVPIGQVFSRFNRLVRDLA
                     KEFDKKVVLEIKGEETELDKKVIDVIGEPLMHLIRNAVDHGVESVEERRQQKKPEIAT
                     ITLNAYQGGNQIFVEVSDDGKGLDLKKIKAKAIEKGLASPEMIANMDKEEIYNFIFTP
                     GFSTSDVVTDISGRGVGMNVVQETVNELNGSVAIESEPGMGTRFILSFPLTLAIIPAI
                     MVRVQRELYAIPLSDVIETIKILPEEITTIEGHEVINLRGEILSLIRLNNFVGIESGL
                     KGNERMPVVVVGYGNRKIGIVVDLLEGKQEIVIKSLEQNYRTVAGLAGASILGDGSIC
                     LILDISSMINKVIMQQDTFSFKEKQRVLGAKQASVEVVKTEVATKEQPVKETVDVQSK
                     KEIGETLLAKEMPIKEDIVFKKEPEEIVEVEEYKEPEKNYELKRGKDTVIEEKVHSVL
                     EEFKTELKEKIKDTLDKKDEIYQKLGISDDDLAKVQVLANVGITDAAESLSKIINRRV
                     DLSIPDVSVLAIESIPQSFGDVDSVYLGVYMPIVGQINGTILFSLREDSGYELVDMLL
                     GLPQGTTDTLTEDGESVLKEVTNIVGSAVVNAFADKIEMAIKPTVPSVVHDYMQSILD
                     SILAFHTAENEYALVMDTEFFHQDDRVIGKLLIMPTTSSLATIVERLRN"
     gene            complement(8162..8659)
                     /locus_tag="PK348_00350"
     CDS             complement(8162..8659)
                     /locus_tag="PK348_00350"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HXK64695.1"
                     /translation="MNNELLNIAAKTAESVIDDTDSLDILQLVSFKLDEIEYGIDILS
                     VHEILRIPEITRLPNTPEYIKGVINLRGNVIPVVDIRLRFGMQQVALTELSRIIVVEI
                     GEKLVGLLVDNVHQVIRLSRSRIDEPHDLIEGISTEFISGIGRLQDRLIVILRLDNIL
                     FSDEQ"
     gene            complement(8897..>9506)
                     /locus_tag="PK348_00355"
     CDS             complement(8897..>9506)
                     /locus_tag="PK348_00355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK64696.1"
                     /translation="TTKGIIYFISKNGTILHKVNCDAPFVTRPVSFGQMVCVVTADGY
                     LYAIDVVLGVSIYRKYIGKVLDNGIFANYPEMYIVTPASIQKIHLLRDEIIWKYDNSG
                     IVAAVEHPQGILFATAHGTLGKIAANGTLEWQINPGKEIRSLQYTPKGIVCIAENVFY
                     QISDAGEVLWSYTLPALNSEILSVTPKTVYIRLGNSFLALRL"
BASE COUNT         2791 a         2088 c         1424 g         3203 t
ORIGIN      
        1 acaacaggta aatgtgaaaa atcttttaac accggaactg cacctgcatc aagagtattg
       61 cgggtaatat cctcaaatgt ccgtatacca cgggggcaaa gtattacctg tttgttgccc
      121 tgtgaaagta tatattctgc tgacatcaag aattcattta tggttgcact catccctcgc
      181 tttaacaaaa ttggcttatt tatttttccc agctccttta aaaggttaaa attttgcata
      241 ttgcgggtac caacctgcaa tacatcagca tactgggcaa caagttctac atcacgggga
      301 tccattacct cggtaattat tggtaaccct gtttcctctt ttgcctttgc taaatacttt
      361 aaccctaacg ccccataccc ttgaaacgtg tacggcgatg accgtggctt gtatgctccc
      421 gcccttagca tggatgcgcc agcctgcttt accatttttg catgctctat aatttccttt
      481 tcaccttcaa cagcacaggg accggccacc agtacgatct tatcaccacc tatatcaata
      541 tcacccacac ggataatggt tctgtcggga tgagtttctt tgcttgctaa cttgtatggt
      601 tttaatattg gcttgaccga ttcaacacct gatattgctt caagtggctg ttcacggatg
      661 atgtcctcat cgccaataac cgtgataatt gtcctgtact gcccctttga gacatgtggc
      721 tgcaagttta acgactttat tttgccaact atatgatcaa tttcatcctg tgtaacattg
      781 ggttttagta ctataatcat aattaccgtg ctcctttttt atgttaaatc tttaatcatg
      841 tttttcagcc tgatatgatc ccagaacctt caaaaatatg gtttcctgtt tcatagcctc
      901 cagcgccttc tggacaacag gatcagttct atgtccatta aaatcaataa aaaagttata
      961 tgcccacatc ttcttcttat cagggcggga ctctatcatg gtcatattaa taccatgttc
     1021 actgaacggt tttagaagct tatacagtgc accaggctga tctttcacag cacacactat
     1081 tgaagttttg tcattacctg ttggcgggtt atcatgcttc cctaacacaa gaaaccgtgt
     1141 atagttttga cgtgaatctt caataccatc agcaatgatg ttaagattat atattttagc
     1201 tgaaatattt gaagcgatag cggctgagta tttatcccat gatgcagttt cagcagcttt
     1261 tgcagttgaa ttcacctgat ttatttccac attgggcaaa tttgctttaa gccagttttt
     1321 acactgagca agcgattggg gatgggaata tattttttta atcttttcga tactgtcaca
     1381 tactgatagc agactataag taatcctgag cgatttttcg gatacaattt ttaaatcagt
     1441 atcaagaaat tcatccatgg tgtaggtgac cgcaccttcg gtactgtttt caattggaac
     1501 tacaccaaat gttgccctgc ctgtctcaac ttcatcaaat acatccgaaa tagtcctgtg
     1561 cggtacagcc tctataacat caccaaaaat ttccagcaga gcaatatgag taaatgttcc
     1621 aactggacct aaatatgcaa ccttcagagg gcgagctgct gaaagagcca cagaaaaaat
     1681 ttccgttgca attttttcta ttaactcagg ggtaagtggt ggccgggcat attgctgcaa
     1741 atgcgaaatt atttttgctt taaccgggaa aatcccctgc tcaccttctt tcatgatgtt
     1801 ctgcaactct ttgctgtata gttccgttaa ctgagacaac gattcaatta tgtttttttc
     1861 aaaactggca gtatccatat cattcctctt caaaattaaa ttcttttatc tctgatagct
     1921 tgggcaggtc agatagttta tttaatccaa aatagcgcaa aaactcatcg gttgtgccat
     1981 ataataacgg ccttccggga acatcttttc tgccaacagg ctttataaga ttttttcgca
     2041 taagtgctgc aaccatcatt cgtgatgaca caccccgcaa ttcttcaatc tctgcaagca
     2101 tgattggctg tttgtaagca atgattgcta atgtctccag catcccttta gacaatgtct
     2161 cttttttctt ataccccatt acctggcgta actggcttgc aaatttagga ttggtgacaa
     2221 attgataccc accagatatc tcaataatct ttattccttt gtcacggtct tcatattcat
     2281 ctataagcga atcaataata atctttgcct gggtagcatc tatacccagc tgcctggtaa
     2341 aaaactgcaa tgggacaggt tcagctgata caaacagtat agcttctact atgccaaaga
     2401 gatcatcact atcgcccatt tcaaatggtt cctcattgcc tgcctcatca tccgggctgt
     2461 cctcgcgatc agattttatt tcaatatctt cattcatgat atcgtcactc atactattta
     2521 cctgattgtg gattttttaa tgcaatgtat ccactttgtt taaatccttt ttaaatatat
     2581 atatattgcc aaattgcgca tgctgcaaca cagcgatagc tcctatacgg gtcatttcca
     2641 gtaaagccag taatgtaaca accacttcca ttcttcccgg attctgcgta aacatatcgt
     2701 gaaattctat tttgtcctta tcttgcaaca tactaaccag taacattatc cggtcactca
     2761 caaggatttc atcaaaaact attcgttgct gctcagtttc cttttctgct tccagtacat
     2821 caacaaacgc attcaaaagg tcaaaaagat ttacttcaat atatacatcc tcctcaaaag
     2881 ataaaggggg aatctgcctt gtaaatacat ctgattgcat ttcatgcaat ttcaataaat
     2941 ttttagatgc tatctggtat tttttatatt ccagaagctt ttgtataagt tccgggggaa
     3001 gcggtggaac aaaaaattca tctcctatat cagcacccgg caacaatgct tttgatttat
     3061 aatataacaa ttcactggcc attacaataa attccatggc aacaggaata ttaatgcttt
     3121 ccattagctt taaatactcc agatattgct ccgtaatatc agcaatagaa acctgggtta
     3181 tttctatctt tgactttttt ataagatccc ataataaatc aaggggaccg tcaaaatttt
     3241 caatgtgtaa aatacattga acatcattgt tgtctggtgt tatcataact caaatagtgt
     3301 taccgcttat tgttataatt tgatatataa atgtacaaaa acagacaatt aatgataatt
     3361 gctatattta tgcaacgata attaatattt ttgaaatagt aaaaaagtaa aaaatattac
     3421 gataaaacat taatgattgt attatgctaa tgctttttca gcagctttct gaagtctttc
     3481 cggagggaat ggtttaacga caaaatcctt tacacctatt tttatagccc gtgttaacaa
     3541 attttcctgc cctaatgcgg tcaccataat tattcttgca ttaggatcaa actgtaaaat
     3601 ttcttctgct gcttcaattc catctttctc acgcatggta atatccattg tcacaaggtc
     3661 aggccgcaac tttttataaa gctgtactgc ttccagtcca ttttcggctt ctccgattat
     3721 ctcatggcct ttgggcacca gtgcatcctt cacaagggtc cgcatgaact tagcatcatc
     3781 aactatcagt attttagcca tagaattacc ccgcagtata aaatatccct attccaaaat
     3841 ccagtacata accttaaata cataataccg atatcccttt atcattcaag aaaaaatttt
     3901 tattaattac aaaattttgc aaattaattt aacagataaa tattcacttc ctaaacacat
     3961 tgaacattgt ataattccac cattatttgt ggggattata cattattcaa tttttataca
     4021 ttaaatatga gaaattatct cctcgggtat tgaatctaca tccacaacag catccactcc
     4081 ccccatctca actgcaaccc tgttcatccc ataaacaaca gacgtggatt catcctgagc
     4141 aatggtgtaa cctccgttat catgaatttc ctttatccct ctggcaccat cacgtcccat
     4201 acctgtcatg atgacaccta taacattatg cgaatcttcc tttgccagag aatgaaacag
     4261 cacatctgct gaaggtctat gaccggaaac tttctctgcc tgtgacaggc gtataacacg
     4321 ggtatcacct gttttttcaa ccacaatatg agcatcgcca ggagctatat acacacaacc
     4381 gttcaatact ttttcaccat cctcagcttc ttttacagat acattgcata aactatccaa
     4441 tcgttcagca aacgctttcg taaaacccgg aggcatgtgc tgtaccacga gtattggtga
     4501 agggaaattt ttaggcaact tttgtaatac tctctgtaaa gctgaaggac caccagttga
     4561 agtgccaata ccaacaatct tttttttatc ttcatgcctt gcaataatag atactttatt
     4621 agtttctgaa acatgttcac tctttgttac atgtaaatta agcgaagcct ttgctactgc
     4681 tttaacttta gtaataagca gttctgcaat atcatccatg ctcatagcca gaacagttga
     4741 aggtttcggg ataaaatcaa ccgcaccata ttccaatgcc ttgaaagttg cttcagcacc
     4801 atattgtgtc aatacactca tcatgataac aggcttggga tttgttttga taatttcctg
     4861 caaagcctca agcccattca taatgggcat ttcaatatcc atggttatga catcaggatt
     4921 taaagtaaga tttttaaata cagccgattt gccattattt gcagtaccaa taacttctat
     4981 ttctttatca ctttcaagaa tatcagaaat tacttttctg accagagcag aatcatcaac
     5041 taccagaacc tttatttttt ctgacataca atttaatttc gcaacctttc cactatggta
     5101 gctaacgaag acgtcgtcgg cataatcaga agcttcccaa ttaccctgtc gtcctggtgg
     5161 aaaaattcag tatccatcac aagcgcatat tcattttctg ccgtgtgaaa ggccagtata
     5221 gaatctagaa ttgattgcat ataatcgtgc accactgaag gcacggtggg ttttatagcc
     5281 atttcaatct tatcagcaaa ggcattgacc actgctgacc ctacaatatt ggttacttct
     5341 tttaatactg actcaccatc ttcggtcagg gtatctgttg ttccctgagg caaacccaaa
     5401 agcatatcca ccagttcata ccccgagtct tctcgtaaac tgaacaaaat tgttccattg
     5461 atctgcccta ctatgggcat atatacacct aaatacacac tgtcaacatc accaaaagat
     5521 tgtggtatag actcaatagc aagaaccgaa acatcaggta tcgacaaatc cactcttctg
     5581 ttaattattt ttgatagcga ttcggcagca tctgtaatgc caacatttgc aaggacctgt
     5641 acttttgcca gatcatcatc tgatatgcct aacttctgat atatctcatc ttttttatcc
     5701 agtgtatcct taattttttc ttttaattct gttttgaatt cttccaaaac actatgaact
     5761 ttttcctcaa tgacagtatc tttccctctt tttaattcgt agtttttttc tggttcttta
     5821 tattcttcaa cttcaactat ttcttcaggt tcttttttaa atacaatatc ttctttgata
     5881 ggcatttctt ttgcaagaag cgtttctcca atttcctttt ttgattgcac atcgacagtt
     5941 tcttttacag gctgttcctt tgtagcaact tcagttttta caacttctac tgatgcctgt
     6001 tttgctccca acacccgctg tttttctttg aatgaaaaag tatcctgctg cattataact
     6061 ttattaatca ttgaagaaat atcaagtatc aagcatatac ttccatcacc caatattgaa
     6121 gcaccagcta agcctgcaac tgtcctataa ttctgttcca gtgattttat aactatttcc
     6181 tgcttccctt ccaatagatc cacaactatc ccaatttttc tgttcccata accaacaaca
     6241 accactggca tacgttcatt gcctttcaat ccgctttcaa tgcctacaaa attgttcaag
     6301 cgtataagtg ataaaatttc acctctcaga ttaattactt catggccttc tatagttgta
     6361 atctcttcag gcaaaatttt gattgtttct ataacatctg aaagtggtat ggcatacaat
     6421 tctctttgca ccctgaccat tattgcaggt ataattgcca gcgttaatgg gaaactcaga
     6481 ataaaccgtg tgcccatacc gggttcactt tcaatggcaa cactaccatt gagctcatta
     6541 acagtttcct gaacaacatt catcccaaca cctctcccgg agatatcagt aacaacatca
     6601 gaagtggaaa atccgggagt aaatataaaa ttatatattt cttctttatc catattagct
     6661 atcatctcgg gtgatgctaa tcctttctct attgcttttg ccttaatttt cttaagatcc
     6721 aaccctttcc catcatcact tacttcaaca aatatctgat tgccaccctg ataggcattc
     6781 agtgttattg ttgcaatttc aggtttcttt tgctgccgcc tttcttcaac ggattcaaca
     6841 ccatgatcaa cagcatttct gattaaatgc atcagtggtt caccaataac atcaattacc
     6901 tttttatcca gttctgtttc ttcaccctta atttccagaa caaccttttt atcaaattct
     6961 tttgcaaggt cccgaaccag tcggttaaac cttgaaaaaa cctgtccaat agggaccatc
     7021 ctggttttca ttatgccact ctgtaaatcc ttggcaatac gtgacatttg ctccatgcgg
     7081 tttttaaatt catttattat actcttttca atattcagac gccgcatttc ttcatacaat
     7141 ttataaaagc ctgaatttgc tataacaagt tccccaacat tgtttaatag ctgatcaagt
     7201 ttttctactg agacctttac cgtccttagt tgcaaagtct tcttatcatc ttttgacctg
     7261 attattattt catctttttc ttcttcatca ggtattacat cttcctcaac atcccttgcc
     7321 ttagtatctg cctcgggttt ttcctcaaaa agtgtttcct gctcatggta tttaagagca
     7381 accttattat ctttttttac cagttcaatt tttctgaggt ctattcttgt aacctgatcg
     7441 acctcacatg ctttctttag ttcctcaact gtagcactgg ttaataaaat aatctgcacc
     7501 ctgttatcaa attctggcat ggaaacttct tcaattgaag gtatagtaat cagaatatca
     7561 gcaatctttt ccagattggt taagaccagt tgtactttta atgatttcat tgcagcatcg
     7621 ggatcaaggg ttatggcaat ttcacagcac gacaaaccat tgttaatccc gtctcgtata
     7681 tctttaataa tattagttga taattgtatc cttttggggg ctggagcttc tttcttttct
     7741 tcctgaatgt tcccttgctt cccaactgat tcaatttttt ttattaaaga tgaaaaatct
     7801 ttcgatggag gattcccgga tgaaacttct cgtattacat cattaataac atcaaaacac
     7861 tgaaataaca catctacaat agcaggtgtt actttcataa ccttgtcacg aataccctgg
     7921 aggagatttt ccatcttatg agccagatcg cccagatcag ttaaacctac aaaggctgca
     7981 gaacttttaa gtgaatgtgc ggccctgaaa atattattta taatctcatc atcagaagga
     8041 tttttttcaa gttttagtaa attctgattg agttcctgca aaagctcatc agcttcttcc
     8101 agaaacatat cctgatattc acctaaggaa aagcccattt tatcacctca aatataaaaa
     8161 atcattgctc atcgctgaat agaatattat caagacgaag gattacaata agcctgtcct
     8221 gcaaacggcc aataccactt ataaattctg ttgaaatgcc ttcaatcaaa tcatgaggct
     8281 catcaattct tgagcgtgac aatcgtatca cctgatgcac attatctacc aaaagaccaa
     8341 ctaatttctc gcctatctcc acaacaatta tcctgcttag ttcagttaat gcaacctgtt
     8401 gcatgccaaa ccgcaagcgt atatcaacca cagggataac attaccacgc agattaataa
     8461 ctcctttgat atattcaggc gtattgggaa gacgtgtaat ttccggtatt cgcagtattt
     8521 catgcacgga taatatatca attccatatt caatttcatc aagcttaaag cttaccagct
     8581 gaagtatatc aagggaatca gtgtcatcta ttacagattc tgctgttttt gcagcaatgt
     8641 tcaataattc attattcata atccaccctt atactatacc tgattatata caattgtata
     8701 tactatgcta tatattcaat ataatttcaa cacaaaatta ttcaactttt aaaaaagttt
     8761 agtatcattg ttataggatc tttgagcctt ttattatgaa ttattatgta aagatattca
     8821 ataaaaaaac gattttttct aaaatgccta tcatttctgt tagcagaaat gtgcggtttg
     8881 acaatttttt tgtaatttaa agcctcaagg caagaaagct gtttcccagg cgaatataaa
     8941 ctgttttagg agttactgat agtatttcag aatttagggc aggtaatgta tatgaccata
     9001 atacttcacc ggcatccgaa atttgataaa aaacattctc tgctatacat actatccctt
     9061 ttggagtata ttgtaatgaa cgtatttctt tgccggggtt tatctgccat tctaacgttc
     9121 cattagcagc tattttacct aatgtaccat gagcagtagc aaatagtatc ccctgaggat
     9181 gttctactgc tgccactata ccactgttat catacttcca tataatttca tcccgtaaaa
     9241 gatgaatctt ctggatagaa gcaggtgtta ctatatacat ttcagggtaa ttggcaaaaa
     9301 taccattatc cagcacctta cctatatatt tcctgtaaat actgacacct aacacaacat
     9361 ctatagcata cagatatcca tcagcagtaa caacacatac catctgacca aatgaaacag
     9421 gccttgtaac aaaaggagca tcgcaattaa ccttatgcag tattgtgcca tttttactaa
     9481 taaaataaat tatgcctttt gttgtt
//
LOCUS       DAOSZP010000007         9313 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_6295_length_9313_cov_3.135774, whole genome shotgun sequence.
ACCESSION   DAOSZP010000007 DAOSZP010000000
VERSION     DAOSZP010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 9313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9313
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6295_length_9313_cov_3.135774"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..523)
                     /locus_tag="PK348_00360"
     CDS             complement(<1..523)
                     /locus_tag="PK348_00360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK64697.1"
                     /translation="MLKSSAQLINLFDLVNDGILIIDGNNYNVLFNNDRAEKFVTKDG
                     TTSILEDEKIKNILRSLQPDKTHAEHISLKINNKIHHYKVNILAIKDEESYLIAFVLT
                     DTTRLFKLHRKQQQLIAQIRKNYFDRFESLKQLADSIAHEVRNPLVSIGGYANLLMRK
                     CEEGIDHSEAKKFL"
     gene            complement(571..1467)
                     /locus_tag="PK348_00365"
     CDS             complement(571..1467)
                     /locus_tag="PK348_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HXK64698.1"
                     /translation="MIYPSYIALYERGELHKRIEALNTIVTHCTLCPHACKVDRRSTT
                     GKCRTGLQALVASYHAHFGEESCLVGTYGSGTIFFANCNLTCIFCQNWDISQMPNGHE
                     VTPEELADMMIRLQHKGCHNINFVSPTHVVHAIIEALPFAIERGLTIPLVYNSGGYDS
                     VETLQLLDGIFDIYMPDFKYMDATAAMELSGAREYPQKAIAALKEMHRQVGDLVIEKG
                     IAKRGLLVRHLVMPENIVRSDLVFKELSAISPHTYVNIMAQYRPEYRAHTHKSLGRRI
                     TIQEYDQAYLWAKQSGLYRFDR"
     gene            1688..2659
                     /locus_tag="PK348_00370"
     CDS             1688..2659
                     /locus_tag="PK348_00370"
                     /inference="COORDINATES: protein motif:HMM:NF016629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HXK64699.1"
                     /translation="MAENIHDKGYKYLFSNPLMVKELLESFVYMDWVKEVDFTQAETI
                     DKTYVNDYYKEYEADIIYKLRFKNTDMYIYLLIEFQSTVDKFIAFRMLQYIVELYREL
                     IYSHNVKKLPVVFPIMIYNGDKVWKAPLTLEELIDIPEILHECLQYVPRFRYYPLIEN
                     ELNPVILQKMMNVISTVFLLETGDDAIFYKTIDRIKDLVQIYTEKGEFLLIRTLLYWL
                     LHYLQSHGIVDGDEKISENLIKTPQEATSMLAQTLKKIKSDLIKEGFNKGIEKGIQKG
                     IKQGKLIGKHEERIAIARNLLKSGIDDKTIIKITKLTKDQLKELKNR"
     gene            complement(2663..3640)
                     /locus_tag="PK348_00375"
     CDS             complement(2663..3640)
                     /locus_tag="PK348_00375"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase family protein"
                     /protein_id="HXK64700.1"
                     /translation="MVINGTHHTLHLAPMAELTTPALRKVVKNFTDNVVLYSEMLASV
                     AIHAKAPHNEPLIRRYDFDNPFFYQLVGNNPDIMANAATILQDYGCDGININMACSAP
                     HILKTGSGASLLCNFNLARSIIAACRKNVHGLLTVKMRAGFHDVNLQFLRQFVSMLQD
                     EGVDAVIIHPRAAKWGFTRKARWDIIEAIVTEVNIPVIGNGDISEPASALHRLQQTHC
                     DGIMIGRAAVQKPWIFAQCEAALRQQSLSLSLNIEEIWIDVLQNIQVMLPQRLHKSRA
                     HRFCAYYHKNVKVCHTLFSSIRQCNEINDMIVLIRNYFKRNEEERINNV"
     gene            complement(3662..4588)
                     /locus_tag="PK348_00380"
     CDS             complement(3662..4588)
                     /locus_tag="PK348_00380"
                     /inference="COORDINATES: protein motif:HMM:NF013694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HXK64701.1"
                     /translation="MVQRYKFINENFLQNDCGNIVVYSNPNESEISTIASDFGIDIHS
                     VHSALDIDELSRFEIEKDYIVLIWKIPTNMKINELSSLNVITIGFFIKDSTMIIISPE
                     TLTYIHEKVHNQIESLFDALFHFQHHTIKHFTEHLKIIKMISKEIQNKINKSIETKHL
                     VQMFNLSEKLIYYLHAIDTNITSLEKLRSYLITNNQRVNFDFLNDIIIDHHQAKKQAE
                     IYTEVFAGLMDARGSIVNNNMNILIKNLTKINVIFLPLNLIAGIFGMSEYSMMTQGIP
                     WYISYGFFAILIMFVSLLLSLLLNKYDERWRR"
     gene            complement(4690..6432)
                     /locus_tag="PK348_00385"
     CDS             complement(4690..6432)
                     /locus_tag="PK348_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020677671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylpolyamine amidohydrolase"
                     /protein_id="HXK64702.1"
                     /translation="MIRIRRLFNPYIPHNIDAFNAVKNIIKEQFPLLAIKKIDEIEDQ
                     LINPLKYKYHTSLFIAEDIHDSIKGVALVLHFTDLGIFYLDYLAVKPGKSSGGIGSAL
                     YQRIRKEASLLNVFGIFLECLPDDPLLCNNPEDVEQNKKRLAFYERFGARPIINTLYE
                     SKVKPDDDCPPYLVFDGLGNYDSIPNKLARNIIQAILERKYNDYCDEKYITRVIESIK
                     DDPVQLRPFRYIHNKTLIHNIVTPHTYTIRLYINDRHHIHHVKDRGYVESPVRVKSIL
                     SELLKSDIFENGKISHYPDKFIYEVHNKNYVDYFKNVCKNLPEGKSVYPYVFPIRNAA
                     RAPRELSVRAGYYCFDTFTPLNKNAYLAARWGVNCVLSAVDDIVSGIPVAYVLTRPPG
                     HHAEHSVFGGFCYFNNVAIAAQRFSKVGKVAILEIDYHHGNGQQQIFYKRNDVLTISI
                     HGHPSFAYPYFSGFAEELGEENGKGFNYNFPLPETISYDKYKHTLLRAVKIIKDFNPD
                     FLIVALGLDVGKGDPTGTWGLVKEDFYTNGGIIATCGIPTLIVQEGGYRNLTIGKNAF
                     AFFMGFYNSFTLKK"
     gene            6850..7794
                     /locus_tag="PK348_00390"
     CDS             6850..7794
                     /locus_tag="PK348_00390"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HXK64703.1"
                     /translation="MVRKNNNNFPNPEGKINVLKRYLHILALLQSPPGTNETWNAARL
                     AQLLSLEEDEKDISDSHVRKYIKDHIENELGISVDRTQGGLITALAEDLDPDMQLEIA
                     RVYSSFVVKDSTRDMILQKFIATNPDRALWTLARVYFAALEKRMIQCSYKTNEGQLLT
                     GWKLCPCYFIFRNNNLYLIVYDVDMKKYLPLVAERIQNIVVCDKKHDISWEIPDVDEL
                     FATSLSAFIDNTNTVEVTIRYSQVVKTIIENIIASLQPAIQKEPNNWHRATFTISDYL
                     YLCKQLVLYGREVEIVSPHKVRQAMIHMLKESLSVYEQ"
     gene            7963..>9313
                     /locus_tag="PK348_00395"
     CDS             7963..>9313
                     /locus_tag="PK348_00395"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXK64704.1"
                     /translation="MPLPKSYRRHHRLFHRAPQPGLDASIRKEIMEYTMNLLQHLEPN
                     ERLLTLCMECLSITSRREIVESLFSEKEKKNRNEFGPNYYEDALGSLHNPSDLASTIM
                     RIVLSTVQKKKWDTVRYKIFMLLKKEYETIAQYKHELDERVAKLAAFFSLTDIDCTIM
                     KLWAIAISIPNSPLKELLSEVNYSELQKRIAIATGLPLAAVRMSLSKKGLLYTSRIIE
                     SINPDGYEHIVLSDEIIEFLNDVASEDLLEKYVKLDTEPTISLQEFSVSEDDKYIVTN
                     LLKSKQPCNILLYGFPGTGKTQFARSIAAHSGHKVFMLVYTSGRESTRHESISSRIMA
                     VTIAMKIAEKNDAILIIDEADELLNTQSLFPFFSFDSGIKGRINDLLDTSNAQCIWIT
                     NSIYGISSSTLRRFNYSITFKHFTSAQRIILWQRILEHSELEKYFTSEIIHELSHKYA
                     "
BASE COUNT         3051 a         1593 c         1695 g         2974 t
ORIGIN      
        1 ataaaaattt ttttgcctcg gagtgatcta tcccttcttc acattttcgc atcaggagat
       61 tggcatatcc acctatggaa acaaggggat tccgtacctc atgagctatt gagtctgcta
      121 actgtttcag tgattcaaaa cggtcaaaat aatttttccg tatctgggca atgagttgct
      181 gctgcttacg atggagttta aataaacggg tagtatcagt taatacaaat gcaatcaggt
      241 aagactcttc atctttaatt gctaatatgt taaccttata gtgatgaatt ttattattta
      301 tttttaagct gatatgttct gcatgagttt tatcaggttg tagcgaacgt aaaatatttt
      361 ttattttttc gtcttccagg atactggttg taccatcttt tgtaacaaac ttttctgccc
      421 tgtcattgtt aaaaagtaca ttgtaattat taccatcaat gatgagtatc ccgtcattca
      481 ccaggtcaaa aagattgatg agttgtgcag aagatttcag catgggtata atatatatgt
      541 aaaaaaaatt gaggtcaata aaaaataaag ttatctatca aacctgtata atcctgattg
      601 ttttgcccac aaataagcct gatcatattc ctgtatggta atacgccttc ccaggctttt
      661 atgggtatgt gccctgtatt caggacggta ctgagccata atatttacat aagtgtgagg
      721 tgaaatagca gatagttctt taaaaacaag gtcactgcgg acaatgttct caggcatgac
      781 taaatgacgt accagcaaac ctctttttgc aatgcccttt tcaatgacaa ggtctccaac
      841 ctgacgatgc atctctttaa gggcggcaat agctttttgc ggatactctc tggcaccact
      901 taattccatt gcagctgtag cgtccatata cttgaaatcg ggcatgtaaa tatcaaatat
      961 accatctaac aattgtagtg tttctaccga atcataacca cctgaattgt ataccagagg
     1021 aatagtaagg ccacgttcaa tggcaaaagg gagtgcctct ataattgcat ggaccacatg
     1081 tgttgggctt acaaaattta tgttatggca gcctttgtgc tgcagccgta tcatcatatc
     1141 agccagttct tcaggggtta cttcgtggcc gtttggcatt tgtgatatat cccagttctg
     1201 gcagaatatg caggtaaggt tacaattagc aaaaaagatt gtccctgagc cgtatgtacc
     1261 aacaaggcat gattcttcac caaagtgtgc atgatatgaa gctacgagtg cttgcaaacc
     1321 agttctgcat ttaccggttg tagaacgcct gtcaactttg catgcgtgag ggcagagcgt
     1381 acagtgggta acgatagtgt tcagcgcttc aatgcgttta tgaagctctc cacgttcata
     1441 taaagctatg tatgatggat aaatcatgga tgctactatt ttcaataagt attatgttta
     1501 ttgataccag gcaatttaat aaacaaaata attaaattag tacatcatgc tttcttttca
     1561 ttatatgcgt ataacaattt taaagattca ttaaaaaaac tcaaaaattt ttatcataat
     1621 ttttcaaatt ttttatcctg aagcgtattt atatataaat acaatagatt tctaccatga
     1681 ggtatctatg gctgaaaata tccatgataa agggtataag tatttatttt ccaatccatt
     1741 aatggtgaaa gaactgttgg aatcatttgt ctacatggat tgggttaaag aagttgattt
     1801 tactcaggct gaaactatcg ataaaacgta tgtaaatgat tactataaag aatatgaagc
     1861 agatattatc tataaattgc gttttaaaaa tactgatatg tatatctatc tattgattga
     1921 attccaatct actgtagaca aatttatagc attcaggatg ttacaatata ttgtagaatt
     1981 ataccgggaa ttgatatatt ctcacaatgt taaaaaattg cctgttgtat tccctattat
     2041 gatatataat ggtgataaag tatggaaagc accactaaca cttgaagaat tgattgatat
     2101 tccagaaata ttacatgaat gcttacagta tgtccccagg ttcaggtatt atccattaat
     2161 tgaaaatgaa cttaaccctg taattcttca aaagatgatg aatgttatat ctactgtgtt
     2221 tttgttggaa acaggtgatg atgcaatatt ttataaaact attgacagga ttaaggattt
     2281 agtgcaaatt tatacagaga aaggcgaatt tttgcttata cgtacattat tatactggtt
     2341 attacattat ttgcaatcac acggtattgt agatggtgat gagaaaatat ctgaaaattt
     2401 gataaaaact cctcaggagg caacctcaat gttagcacaa acattaaaaa agataaaatc
     2461 agatttaata aaagaaggat ttaataaagg gattgaaaaa ggaattcaaa aaggaattaa
     2521 acagggcaag ttaataggga agcatgagga aagaattgct attgcacgca atttattgaa
     2581 aagcggaatc gatgataaaa caattatcaa aataactaaa ttaacaaaag accagttgaa
     2641 agagctaaaa aataggtaac cattatacat tgtttattct ttcttcctca tttcgtttaa
     2701 aataatttct tataagtaca atcatatcgt ttatttcgtt acattgtcgt attgatgaaa
     2761 acaatgtgtg gcaaactttt acatttttgt gataatatgc acaaaaacgg tgtgccctgc
     2821 ttttgtgaag cctctgcgga agcattacct gtatattttg taatacgtct atccatattt
     2881 cttcaatatt aagcgatagc gacagcgatt gttgccgcaa tgcagcttca cactgtgcaa
     2941 atatccatgg tttctgtacc gctgctctgc caatcatgat gccatcacag tgggtttgct
     3001 gtaaacgatg tagtgctgat gctggttcac tgatatcgcc attgccaata acagggatgt
     3061 ttacctcagt tactatcgcc tcaatgatat cccagcgagc tttgcgagta aatccccatt
     3121 ttgcagcacg tggatgaata atgactgcat ccacaccttc atcctgcagc atactaacaa
     3181 attgacgcaa gaattgaagg ttaacatcat gaaagccagc acgcatctta acagtaagaa
     3241 ggccgtgaac attttttctg caagctgcaa taatagatcg tgccaggtta aaattgcaaa
     3301 gcaggcttgc accactgcca gttttaagaa tatgcggtgc agagcatgcc atgtttatat
     3361 taatgccatc gcatccgtag tcctgtaaaa ttgttgcagc gttggccata atgtcaggat
     3421 tattcccaac cagctgataa aaaaacggat tgtcaaaatc atagcgccgt attaaaggct
     3481 cgttatgggg tgcttttgca tgtatagcaa cacttgcaag catctcagaa tacagaacaa
     3541 cgttatcagt aaaatttttg acaaccttac gcagcgctgg tgtggtaagc tctgccatgg
     3601 gggctaaatg cagtgtgtga tgtgtgccat taataaccat agtatataaa gcatgttgtt
     3661 tttatcttcg ccagcgttcg tcgtatttgt ttagtagcaa gcttaacaac aaactaacga
     3721 acataattaa aattgcgaaa aacccatatg agatgtacca tggtatcccc tgggtcatca
     3781 ttgagtattc tgacatgcca aaaataccag ctattaaatt taaaggtaga aatattacat
     3841 ttatctttgt tagattttta ataagaatat tcatattgtt gttcacaatg cttccacggg
     3901 catccataag accagcaaaa acttctgtat aaatttctgc ctgctttttt gcctggtggt
     3961 ggtcaatgat aatatcattt aaaaaatcaa aattaacacg ctgattatta gttataagat
     4021 atgaccgtaa tttttcaagg gaagtaatgt tggtatctat tgcatgcagg tagtatatta
     4081 acttttcgct gaggttaaac atctgaacaa ggtgttttgt ttctatagat ttgttaatct
     4141 tattctggat ttcctttgaa atcattttaa tgatttttag atgttcagta aaatgtttta
     4201 ttgtatggtg ttgaaaatga aacagtgcat caaaaagtga ttcgatctga ttgtgtactt
     4261 tttcgtgaat atatgttaaa gtttcaggac tgatgataat cattgtactg tcttttatga
     4321 agaagcctat agttataacg ttaagggatg aaagttcatt aatcttcata tttgttggta
     4381 ttttccatat aagaactata taatcttttt caatttcaaa tcgtgatagc tcatcaatat
     4441 cgagagcaga atgaaccgaa tgaatatcta tgccaaaatc actggctata gtggatattt
     4501 cactttcatt aggatttgag tatactacaa tgttgccgca atcattttgt aagaaatttt
     4561 catttataaa tttatatctt tgaaccatat gtcctccata cgtaaaaaga ataattagta
     4621 aagaactaca ttgcaggaaa aaaataaata catgaaggat tcagtactat atgtagtata
     4681 aaatttaatt tattttttca atgtaaaact attataaaat cccatgaaaa acgcaaatgc
     4741 atttttccct atagtaagat ttctatagcc accttcctgt acaatgagtg ttggtattcc
     4801 acatgttgct attattcctc cattggtgta gaagtcttct tttaccagac cccatgttcc
     4861 tgtaggatcg ccttttccca catccaatcc cagggctaca attagaaaat cagggttaaa
     4921 atcttttata atcttcactg cacgtagcag ggtgtgtttg tatttgtcat atgagatagt
     4981 ttcggggagg gggaaattgt aattaaaccc cttgccattt tcttcaccaa gctcttccgc
     5041 aaaaccggag aaataaggat aggcaaacga aggatgacca tgtatggata ttgtgaggac
     5101 atcatttctt ttataaaaaa tttgctgttg cccattgcca tgatgataat ctatctccag
     5161 aatagcaacc ttgcctactt tactgaatcg ctgagctgca atagcaacat tgttaaaata
     5221 acaaaaacct ccaaatacag aatgctctgc atgatgaccg ggagggcggg tcaggacata
     5281 agctaccggg atgcctgaga caatatcatc aacagcagat aagacgcaat taactcccca
     5341 tcgtgctgct aaataagcat ttttattgag tggtgtaaat gtgtcaaagc agtagtatcc
     5401 tgcacgtact gaaagttccc tgggtgccct ggcagcattt ctaattggga aaacatacgg
     5461 gtataccgat ttcccttcag gtagattttt acatacattt ttaaaatagt ccacataatt
     5521 tttattgtgt acttcatata taaatttatc aggataatgt gatatttttc cgttttcaaa
     5581 tatatctgat tttaataatt cggacaatat gcttttgact cttacaggtg attcaacata
     5641 tcccctgtct tttacgtggt gtatgtgatg cctatcgttt atatagagtc taatggtgta
     5701 tgtatgagga gttacaatat tgtgtattaa ggttttattg tgaatgtatc taaatggccg
     5761 tagttgtaca ggatcatcct ttatactttc aataacacgt gtaatgtatt tttcatcaca
     5821 atagtcgtta tattttctct caagtattgc ctgtataata tttcgtgcca atttgtttgg
     5881 tatagaatca taattcccca atccgtcaaa aaccagatat ggaggacaat catcatcagg
     5941 tttaactttg gattcataga gagtatttat tatgggacgt gctccaaaac gttcgtaaaa
     6001 cgccaatctt tttttatttt gttcaacatc ttctggatta ttacacagta aaggatcatc
     6061 aggaagacat tccagaaaaa ttccaaaaac attcagcaag ctggcttctt tacggattcg
     6121 ctgataaaga gctgaaccaa tacccccaga cgattttcct ggtttaacag caagataatc
     6181 aaggtagaaa atgcccaaat cagtaaaatg caaaaccaga gcaacgcctt tgatagaatc
     6241 atgtatgtct tcagcaataa ataatgatgt atgatattta tatttaaggg ggttgatgag
     6301 ttgatcttca atctcgtcaa tttttttaat tgccagaagc gggaattgct cttttatgat
     6361 attttttact gcattaaaag catcaatatt atgaggtata tacggattga acaatcggcg
     6421 tatacgtatc atagtttttc ctttgattag ttttttattt acattgtatc atacatgtta
     6481 atagtgattg tgtatctttc acagatagtg ctattatata gtatagtata taatatataa
     6541 caagatttac aaaaatattt cacaaattgc catcagagta ttttatgatt aaagcattat
     6601 gcacaataaa aggtatggca agtgaatatt tttaaacatt gtgttctata ttattaacct
     6661 ggttgaagtt taaaaattaa ttttgaagga tttagatgat tacttcttaa aacaattttg
     6721 ctaataaatt tttctgtcag tttatagaga gtatatgatt tgaccaggga tatgcaaact
     6781 tttctgcagt aaaaagaatt caaaaaaata gtttacaaac aatttcgtac tgtgaaacat
     6841 atgtgttcaa tggttcgtaa aaacaataac aacttcccta accctgaagg caagatcaac
     6901 gtattgaagc gctaccttca tatactggcg cttctgcaat caccacctgg aaccaacgaa
     6961 acctggaatg cagcaagact tgcgcagtta ctatcgcttg aagaagatga aaaagacatt
     7021 agcgacagcc atgtccgaaa atacatcaaa gaccatatag aaaatgagtt aggaataagt
     7081 gttgaccgta cacagggcgg actcataaca gcacttgccg aagaccttga tcctgacatg
     7141 cagcttgaaa ttgcgcgagt ttatagcagc tttgtggtaa aggactcaac gcgtgatatg
     7201 atattgcaaa aatttattgc aacaaatcct gatagggcac tatggacatt ggcacgcgtc
     7261 tattttgcag cgcttgaaaa acgcatgata caatgtagct acaaaaccaa tgaagggcag
     7321 ttactaacag gctggaaact gtgtccctgt tattttatat ttcgtaacaa caacctgtat
     7381 cttatagtgt atgatgtaga tatgaaaaaa taccttccgc ttgttgctga gcgcatacag
     7441 aatattgtgg tatgtgataa aaaacatgat attagctggg aaatccctga tgttgatgag
     7501 ttatttgcaa cgtcactgag tgcattcatt gataacacca atacagtgga agttaccatt
     7561 cgctattccc aggtagtaaa aacaatcatt gaaaatatta ttgcctcgtt acagcctgca
     7621 atacaaaagg agcccaataa ctggcacaga gcaacattta ccataagcga ttatctttac
     7681 ctgtgcaaac agcttgtgct gtatggaaga gaagtagaga tagtaagccc acacaaggta
     7741 cgacaggcca tgatacacat gcttaaggaa agcttaagcg tgtatgaaca ataaacatcg
     7801 caagtcagtt gctacctttt ttactatgct tatagtattt taccaataaa taacctgcca
     7861 tacgtagtct aaatgtagta gcgatagtta ctcattaagg atctatcttc aacaaccacg
     7921 gtgttaacaa agtaccctat attaccatta caggaggtag ctatgccttt accaaaatca
     7981 tatcgacgtc accatagact ttttcacaga gcaccacagc caggactgga tgcttcaata
     8041 cgtaaagaga taatggaata cacaatgaat ttactgcagc atttagaacc aaacgaacgc
     8101 ttgctaacac tgtgtatgga atgtttaagt attacaagcc gcagagagat agtagaatca
     8161 ctgttttctg aaaaagaaaa gaaaaaccgt aatgaatttg gtccaaatta ttatgaagat
     8221 gcattaggga gcttgcataa tccttcagac ttagcttcta caattatgcg gattgtactg
     8281 agtactgtgc aaaagaaaaa gtgggataca gtacgttaca agatttttat gttattaaaa
     8341 aaagaatatg aaactattgc acagtataaa catgaattag atgagcgcgt agctaaatta
     8401 gcggcatttt tttcactcac tgacattgat tgcactatta tgaagctatg ggctatagct
     8461 atttcaatac caaacagccc gttaaaagaa ctgttgtctg aagttaatta cagtgaattg
     8521 caaaaacgca ttgccattgc tacaggattg ccgcttgctg ctgttagaat gtccctatca
     8581 aaaaaagggc ttttatatac ctctcgcatc attgagtcta taaatcctga tggttatgaa
     8641 catatcgtgc tgtcggatga aataattgaa tttttgaatg atgttgcaag tgaagatttg
     8701 cttgagaaat atgttaaatt agatactgaa ccaacgatat cgttacaaga attcagcgta
     8761 tcagaagatg ataagtatat agtaacaaat ttattaaaaa gcaaacaacc ctgcaatatt
     8821 cttctatatg gattcccggg aacaggtaaa acccagtttg cacggagcat agctgcgcat
     8881 agtggccata aagtctttat gcttgtctat accagtggac gcgaaagtac caggcatgaa
     8941 agtataagtt cgcgcattat ggctgtaacc atagctatga agatagctga aaaaaatgat
     9001 gcaatcctga ttattgatga agcagatgaa cttcttaaca cacaatcgtt gtttccattt
     9061 ttttcttttg attcaggcat taaaggcagg attaatgatt tgcttgatac cagtaatgcg
     9121 cagtgtatct ggataactaa ttctatatat ggaatatcat cttcaacatt gcgccgtttc
     9181 aactacagca taacgtttaa gcacttcact tctgcacagc gtatcattct atggcaaaga
     9241 atactcgagc acagtgagct tgaaaaatat ttcaccagtg agataatcca tgagctatcg
     9301 cataaatatg ccg
//
LOCUS       DAOSZP010000008         9166 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_6436_length_9166_cov_3.093404, whole genome shotgun sequence.
ACCESSION   DAOSZP010000008 DAOSZP010000000
VERSION     DAOSZP010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 9166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9166
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6436_length_9166_cov_3.093404"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..160)
                     /locus_tag="PK348_00400"
     CDS             complement(<1..160)
                     /locus_tag="PK348_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HXK64705.1"
                     /translation="MKRYTLPQLPYEYNALEPYIDAKTMEIHHTKHHATYIDKLNAAL
                     DKYPHLYEL"
     gene            375..479
                     /locus_tag="PK348_00405"
                     /pseudo
     CDS             375..479
                     /locus_tag="PK348_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012451449.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            complement(468..1016)
                     /locus_tag="PK348_00410"
     CDS             complement(468..1016)
                     /locus_tag="PK348_00410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK64706.1"
                     /translation="MLRKKIKYLTTLINNYYFDENNNLVIQYQMIIAIIGSIVLMVGV
                     FLPFIKAPFLGVVDYTRIGKYYDEIVIACGLVAIILALLKLYRWLLIPAFGSLCVIVF
                     SIYKVFSKVSMLREMVEDKLYNNPYKDLIATFIDSMQEATSLQWGVIILGTGVLLLLL
                     AGMLQTKNIVIPKKVNSPQSNM"
     gene            1579..2562
                     /gene="selD"
                     /locus_tag="PK348_00415"
     CDS             1579..2562
                     /gene="selD"
                     /locus_tag="PK348_00415"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: protein motif:HMM:NF002098.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HXK64707.1"
                     /translation="MGPADLAQVMKKLPIEYNSDVLVGMQTSDDAGVYRISDDVALVQ
                     TVDFITPIVDDPFVFGQIAATNSISDVYAMGATPITAMNIVCFPITTFAMDVLEKILQ
                     GGLDVMKKTGVQLIGGHSIDDIELKYGLSVTGITHPNTIFTNTGLQPDDVIILTKPIG
                     TGTINTAIKAGIAANEHIDRAIQSMTTVNDCLSGYPHREHIHAVTDVTGFGLIGHLSE
                     MLGTGSIAIHVHANDVPLIPGATDYAAMGLLPAGLYRNRDFVGSRCSVASGVKRDIAD
                     LLFDPQTSGGLLIATSKHTAHQVIEYIAAKALCTAIISTCSFSQEGHIYIE"
     gene            2583..2954
                     /locus_tag="PK348_00420"
     CDS             2583..2954
                     /locus_tag="PK348_00420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK64708.1"
                     /translation="MKYSDDKRQQLLSKVIWDYNLDIKEIIKTIDNDTITHNKKQIFV
                     RCLQRLPWHNVVGIFGFDAAKTLLTDDVIEQIWPKERREHFATLQKLLRGEPLPSARW
                     DSELREKLKSTVLSYRWYRFQ"
     gene            3040..3132
                     /locus_tag="PK348_00425"
     tRNA            3040..3132
                     /locus_tag="PK348_00425"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3074..3076,aa:Sec,seq:tca)
     gene            3154..4488
                     /gene="selA"
                     /locus_tag="PK348_00430"
     CDS             3154..4488
                     /gene="selA"
                     /locus_tag="PK348_00430"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNASec selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HXK64709.1"
                     /translation="MYEQIPQVQKLLEEDSIRQFIGEIGHPFVVAVIRETLEEQRKLI
                     AKGSPFDYGKCVEAIAHRCMAIRMEKLQRVINGTGILLHTNLGREPLGKDILSSVTEN
                     LSAYCNVEFHLPTQKRGKRGGFAEKLICLITGAEDALIVNNNAASVFLILHHFARNKE
                     VIISRGELIQIGGGFRIPDIMKQAGCHLVEVGTTNITDVSDYKNAMTENTALILSAHR
                     SNFYMRGFTQNPTLQQLATLKSDRVLLVKDLGSGNLIFNNDDNVTQALNYADLVCFSG
                     DKLLGACQAGIIAGKKDLIATLKKDPLMRIVRVDKVTYFILQQVLLHYANNKHGVIPA
                     WSFMKQDLHAIDKKIKKLFKKLKHEVKPYIQRINSKTAFGGGAMPDKVLPDYGIAIAI
                     DGKKASDIATHMVSQPVPIIGYIQNDVFTINFRTILDDDIGNVADAINSLQP"
     gene            4498..6240
                     /gene="selB"
                     /locus_tag="PK348_00435"
     CDS             4498..6240
                     /gene="selB"
                     /locus_tag="PK348_00435"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HXK64710.1"
                     /translation="MYVIGTAGHVDHGKTSLIKALTGIDADRLPEEKQRQMTIDIGFA
                     FIKLPTIGTVSIVDVPGHERFIRNMVTGAWGIDIGLLVIAADDGWMKQTDDHVKVLSI
                     LGIPSIVIALTKIDKVAPEDITRVLSHIHNKLITTPYRNANVIPCSSITGQGIDTLKA
                     ALEDAIHRLPQPEAGNKPYLHIDRVFTVKGVGTVVTGTSKNGSFMVNDSITILPHTIT
                     TRIRQIQSHNTQQEKSDVSQRTALALAGVEKATLQRGHIIVKENFFTETRSCTALITH
                     TFSPIKNNQWIEVLIGTAACDAQYSVIGQSNTVVRLKFKEPVYCYPTERFVATFPGGY
                     TIIAGGYILSTDYKGAIKKDPLLQKLSQSNDTTANAILCTLITNPYMSIEHLKKIFPD
                     EAHVVDTIERLKSNSQVTIIGNYISTTSHYDTTVNIVKQECASDINEQELAQKLLLPY
                     DVVHHIVTHHGLLKQKKDEIGDSEREFLQQVLQKGIEGINPKDFPRHRNIIDKLIKTE
                     HLVVIDKELIWHSDVFTQYTHRIMAHFEKNKELTIQQAKEITGLSRKYMIPLLNAMEQ
                     KNLIKRYGDVRIKV"
     gene            6349..7383
                     /locus_tag="PK348_00440"
     CDS             6349..7383
                     /locus_tag="PK348_00440"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HXK64711.1"
                     /translation="MALFTPNVDKLREKNDIKGLLQLTKHRKAEVRLNALLALVKIRD
                     QSVIEDLKKLIHDPDPRVRTIATIKFGNIKDPVIIENLRQIIISGSQRDKIEALRVLS
                     EIGPTDDLEISKILYLAFKDKKMMVSIEAIRTMGYLKDKYAIPHLVEALDSKHYQIRV
                     EATRALGMIGGDQAVSPLIGCLVDNNAEVRKTAHDALKKIGTERALKAITDAPFMLLV
                     KRMTEGGAQREETVKQIGQFKIKEAAPLLIKACNDEFKSVRMSAARSIGILREKSAIG
                     ALVKLLDDPYYDVRLEAVRALEKIFDPQALKAIEKAMSDPNKNVRDEARVCYYSLKSR
                     LERVHDTEQV"
     gene            7390..8154
                     /locus_tag="PK348_00445"
     CDS             7390..8154
                     /locus_tag="PK348_00445"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HXK64712.1"
                     /translation="MIIGFNIGNTHTVVGLYEAGNPLPVFMDRYPTKREVTVQELSDT
                     ITSAINKYSVDAGAMQGAIYSSVVPQCNDAYDAVCKKLFSINPHRITYRSRMPITIVY
                     DNPAQLGVDRIVNAVAAHIDYNTDCIIVDIGTAITFCLLEHGVLKGGIIAPGVKAAMK
                     GLAQSTAQLFEVPFEKPASIIAQNTKDALISGFFYGWISLIEGIIQKMAGAIAHYTVI
                     CTGGMAEVLQPYLSIPANIDTTLTMRGLYYLYDINR"
     gene            complement(8754..8936)
                     /locus_tag="PK348_00450"
     CDS             complement(8754..8936)
                     /locus_tag="PK348_00450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK64713.1"
                     /translation="MENTNRLYQYLLQKKKSFAYVSDEGIDGITIMIHARPGKSKGWK
                     SSYEVLQGKLNGALQT"
BASE COUNT         2982 a         1781 c         1745 g         2658 t
ORIGIN      
        1 atagctcata cagatgagga tatttatcca gtgctgcatt gagcttgtca atgtatgttg
       61 catggtgttt ggtgtggtga atttccatgg tttttgcatc aatataaggt tcaagagcgt
      121 tgtattcata tggcaattgt ggtaatgtat atcgtttcat ggtttttctc cttttcaagc
      181 attgtagtat taactaaatt attgtaatat acccattatt tttgttttcg tcaataggta
      241 aaaaacattt tgggcgatag ttactgtaaa aaagcctttt aacaattact caacttttat
      301 taataattaa taaatgaatt ttcaataact atcgcattaa aaatataaag gcaatactac
      361 ttaaaacttt ttataaaacg ctaaaaatat atgatgacct gtatcatgaa attttgaatg
      421 aacctgaaaa ggttttggta ataggtgata tagtgcactg gttagattca catatttgat
      481 tgtgggctat ttactttttt tggtataact atatttttgg tttgcaacat tccagccaat
      541 agtaaaagca acacacctgt tcccagtata attacgcccc actgcaatga tgtagcttcc
      601 tgcattgaat cgataaatgt agctatgaga tccttatagg gattattata taatttatct
      661 tcaaccatct cacggagcat gctaacctta ctgaatactt tgtatattga aaatacaata
      721 acacagagcg atccaaaagc aggaatgagc aaccatcgat atagtttgag caatgcaagt
      781 atgatagcaa ccaaaccaca tgcaattact atctcatcat aatatttccc aatccgtgta
      841 tagtctacca cacctaaaaa cggtgctttt atgaaaggaa gaaaaacacc caccattaaa
      901 acgattgacc caataattgc aattatcatt tgatactgaa tgactaaatt attgttctca
      961 tcaaaatagt aattattaat cagtgttgtt aaatatttta tttttttccg taacataaca
     1021 ttaaaaactt cctcacctta atactttttt attttttata aaaatatctt atatattaat
     1081 atagcaatca tgcaccgaca atacatcaca aagcatatca aatatgtcta acgaagcttt
     1141 gtatttttac tagaatacta tacgttctac cagataatta tatttaagga gcttaatgct
     1201 agaggcatac tgtaattatg aagaagagta ttgcaagcac aaaatgttca ctcaatcgat
     1261 ttggacaaat aatgcggcga aataacaata tgtaatgacg agagtagcat gtcataaaca
     1321 gagctaacag ttgaataaaa aagttaattg aatagtaaat aatttcatat atctgatagg
     1381 atcatacatc aaattgaact tgactctgaa aaaaatacct ttttttcttg aaagatatta
     1441 tttattctgt cacaatacat tatcaatttt ttatattcaa ttaaaagatt aattacaatg
     1501 tcaagctgtg atatcaacaa accaaagaaa gatgtgcgtc ttactgagtc tatacgcggt
     1561 tcaggatgag cggcaaagat aggtccggcg gaccttgctc aagtcatgaa aaagctgccc
     1621 attgaataca acagcgatgt actggtgggc atgcaaacca gcgatgatgc cggtgtgtac
     1681 agaatcagcg acgatgtagc acttgtccag actgtcgatt ttattacccc gatagtggat
     1741 gatccttttg tctttggcca gattgctgcc accaatagca tctctgatgt ttatgctatg
     1801 ggcgccacac ccattactgc catgaatatt gtgtgctttc ccattactac atttgctatg
     1861 gatgtactgg aaaagatttt gcagggtggg ttagacgtaa tgaagaaaac aggtgtgcag
     1921 cttattggcg gacacagtat tgacgatatt gagcttaaat atgggctttc ggtaactggc
     1981 attacgcatc ccaatacaat ttttaccaac acaggattac agcctgatga cgttatcatt
     2041 cttaccaaac ctattggcac tggaaccatt aatactgcta taaaagctgg cattgctgcc
     2101 aatgaacaca ttgacagagc tatccaatcc atgactacag taaatgattg cctgtcaggc
     2161 tacccgcata gagagcatat tcatgcagtt actgatgtca ccggctttgg ccttattggg
     2221 catctatctg aaatgttggg cacaggttca attgccatcc atgtacacgc taacgatgtg
     2281 ccactcattc caggtgcaac cgactatgct gccatggggt tacttcctgc tggattatac
     2341 cgcaacaggg attttgtagg ttcacgctgt tcggtagctt ctggtgtcaa acgtgatatt
     2401 gctgaccttt tgtttgaccc acaaacgtcc ggtgggctac tcattgcaac atctaaacat
     2461 acagcacatc aggtaattga gtatattgca gcaaaagcgt tatgcacagc aattatttca
     2521 acctgttcat tttcacagga aggtcatata tacattgagt aatgtaccgg taactatcgc
     2581 ccatgaaata ttcagatgat aaacgacaac agttactttc aaaggtaatc tgggattaca
     2641 atcttgatat caaagagata attaaaacta ttgacaatga taccataaca cataataaaa
     2701 agcagatatt tgtacggtgc ttacagagac ttccatggca taatgttgtg gggatttttg
     2761 ggtttgatgc agccaagact ttattaaccg atgatgtaat tgagcaaata tggccaaagg
     2821 aaaggagaga gcattttgca acacttcaaa agttattacg aggggagcct ttaccctctg
     2881 caagatggga ttctgaactg cgtgaaaaac ttaaatctac cgttttatct taccggtggt
     2941 accgctttca gtaggtaatg acaattccct ctgtacataa aaaatccttt acaattattg
     3001 cccatacttt gctataataa accacatagt gtgtaaaaag gaagcggatg agatctggtg
     3061 gtctctgcgg tcttcaaaac cgttggctgc ctctaaaagg cagtggcagg ttcgattcct
     3121 gccccttccg catttttttt aaaggataac gcaatgtacg aacagatacc acaggtacaa
     3181 aaactgctgg aagaagattc tatacggcaa tttattgggg agatagggca tccgtttgtt
     3241 gttgccgtta tccgtgaaac gctggaagaa caaagaaaac tgattgctaa agggtcgcca
     3301 tttgattacg ggaagtgtgt ggaggcgata gctcatagat gcatggctat ccgtatggaa
     3361 aaactgcaac gtgtgataaa tggcactggc atcctgcttc acaccaacct ggggcgggaa
     3421 ccacttggca aggatatatt atccagtgtt accgaaaatt tgagtgccta ctgcaacgta
     3481 gaatttcact taccaacaca aaaacgggga aagcgtggcg gttttgctga aaagcttatt
     3541 tgccttatta ccggtgccga ggatgcactc attgtcaata ataatgctgc aagcgtattt
     3601 ttaatactgc accattttgc ccgtaataaa gaagttatca tttcgcgtgg cgagttaata
     3661 cagattggcg gtgggttccg catccctgat atcatgaagc aggccggatg tcatcttgtt
     3721 gaggttggca caaccaatat tactgacgtt tctgactaca aaaatgcaat gacggaaaac
     3781 acagcgctta tcctttcggc tcatcgatca aatttttaca tgagaggatt tactcaaaac
     3841 cccaccctgc aacagttagc caccttgaaa tcagacaggg tgttactggt taaagattta
     3901 ggaagtggca accttatttt caataatgat gacaatgtaa cacaggcact taactatgct
     3961 gatttagtgt gttttagcgg cgataaacta cttggtgcat gtcaggctgg catcattgca
     4021 ggcaaaaagg atttaattgc aacattaaaa aaagatcctc tgatgcgaat tgttcgcgtg
     4081 gataaagtaa cctactttat tttacagcag gtattattgc attatgccaa taacaagcac
     4141 ggggttattc cagcatggtc atttatgaag caggatttac atgcaataga taaaaaaata
     4201 aaaaaacttt tcaaaaaact aaaacatgag gttaaaccat acattcagcg tatcaactca
     4261 aaaaccgcat ttggcggcgg cgccatgccc gataaagtac tgcccgatta tggcatagcg
     4321 attgctatag atgggaaaaa agcaagtgac attgcaacac acatggtttc tcaacctgtt
     4381 cctattattg gttatattca aaatgatgta ttcaccataa atttcagaac catactggat
     4441 gatgatattg gcaatgttgc tgatgcaata aattctttac aaccatagga aataaccatg
     4501 tacgttatcg gcactgcagg ccatgttgat cacggcaaga catcgctcat caaggcactt
     4561 accggtatag acgcagatag actgccagaa gaaaaacagc gccagatgac cattgatatt
     4621 ggttttgcct ttattaaact tcccactatt ggcaccgtga gtattgttga tgtacccggg
     4681 cacgaacgat ttatacgaaa tatggtaacc ggtgcgtggg gcattgatat tgggctatta
     4741 gtgattgctg ccgatgacgg ttggatgaaa caaaccgatg accatgtcaa agtgctgtca
     4801 atactgggga taccttccat tgttattgca ttaaccaaga ttgataaagt agcacctgag
     4861 gacattaccc gggtactatc gcacatacac aataagctaa tcacaacacc atatagaaac
     4921 gcaaacgtta taccgtgctc atcaataaca ggacaaggta ttgacacatt aaaagcagca
     4981 ctggaagatg ccatccacag gctcccccaa cctgaagctg gcaataaacc ctatttacat
     5041 attgataggg tctttaccgt taaaggcgtt ggtacggtag taaccggtac cagcaaaaat
     5101 ggtagcttta tggtaaatga ttctataacc attctgccac ataccatcac cacacgcatc
     5161 cgccagatac agagccataa tacccagcag gaaaaatcgg atgtttcaca gcgaacggca
     5221 ctggcactgg caggagttga aaaagcaaca ctacaacgtg gccacatcat agttaaggaa
     5281 aactttttca ctgaaaccag aagctgcact gccttgataa cgcatacatt ttcaccaatt
     5341 aaaaacaatc agtggattga ggttttgatt ggaactgctg cctgtgatgc tcagtacagt
     5401 gtcattggtc agtcaaacac tgttgtgcgc cttaagttta aagaaccagt ttactgctac
     5461 cccactgagc gatttgttgc aacatttcca ggaggatata ccatcatagc tggaggttat
     5521 atcctgagta cagattataa aggtgcaatt aaaaaagacc ctctacttca aaaactttca
     5581 caaagcaatg atacaacagc caatgctatc ctgtgtactc ttatcaccaa tccgtatatg
     5641 tcaattgaac atctaaaaaa aatatttcct gatgaagccc acgttgttga cacaatagaa
     5701 aggcttaaaa gcaactcaca agtcactatt attggcaatt atatttccac aacatcacac
     5761 tatgatacta cggtaaatat agtaaagcag gaatgtgcat cagacataaa tgagcaggaa
     5821 ctggcacaaa agcttttgct gccatacgac gttgtgcacc atatagtgac acatcatggc
     5881 ttgcttaaac agaaaaaaga tgaaattggc gatagtgaac gggaattttt acagcaggtg
     5941 ctacaaaagg gcatagaagg cattaaccca aaagattttc cccgtcaccg gaatattatt
     6001 gataaactta ttaaaaccga acatttagtg gttattgata aagaactgat atggcacagt
     6061 gacgtgttca cccaatacac acaccgtatc atggcacatt ttgaaaaaaa caaagagtta
     6121 accatacaac aggcaaaaga aatcaccggg ctttcacgaa aatatatgat accgcttttg
     6181 aatgccatgg agcagaaaaa tctcataaag cgatatggcg atgttcgtat caaggtttag
     6241 ccgtatatag atttgcataa tacaataaaa ttgtacatta ttagttgact ttttttatgc
     6301 atatggagta cacttgtaat aaatatacat acttaaaaag ggattgctat ggcacttttt
     6361 accccaaacg ttgataaact cagagaaaaa aatgacatta aaggattatt acaattaaca
     6421 aagcaccgca aagccgaggt ccggctcaat gctttgcttg ctctggtaaa aatacgtgac
     6481 cagtcagtta ttgaggattt aaaaaaactt atccatgacc cggacccacg ggtaaggaca
     6541 atagcaacaa taaaatttgg caatataaaa gaccctgtta ttattgaaaa tctgcgtcag
     6601 ataatcatat caggttcaca gcgtgataag attgaagcgc tgagagtttt gagtgaaata
     6661 ggccccaccg atgatctgga gatatcaaaa atactgtatt tagcatttaa agacaaaaag
     6721 atgatggttt caattgaagc catccgcacc atgggctatc ttaaggataa atatgccata
     6781 ccgcatttag ttgaggctct tgacagcaag cattatcaga tacgcgttga agcaacccgc
     6841 gcactgggca tgattggcgg cgatcaggct gtcagtccat taattggctg ccttgttgat
     6901 aacaatgctg aagtaagaaa aactgctcac gatgcactga agaaaattgg aaccgaaagg
     6961 gcactcaaag ccattaccga tgcaccgttt atgctcctgg taaagcgtat gaccgaagga
     7021 ggtgcccagc gcgaggaaac agttaaacag attggccagt ttaaaatcaa agaagcagca
     7081 ccactgctta tcaaggcttg caatgacgaa ttcaaatcgg tacgcatgtc agctgcgcgt
     7141 tccattggca tattacgaga aaaaagcgca attggcgctt tagtcaagct tttagatgac
     7201 ccttactatg atgtgcgctt agaagcggtg cgtgcacttg aaaagatatt tgacccgcag
     7261 gcgcttaaag ccatagaaaa agctatgagt gatcccaaca aaaatgtgcg cgatgaagcc
     7321 cgcgtgtgtt actactcgct taaatcccgc ctggagaggg tacacgacac cgaacaggta
     7381 tagcatttca tgattatagg tttcaatatt ggcaatacac ataccgtggt agggctttat
     7441 gaggcaggca accccctgcc tgttttcatg gaccgctatc ccacaaaacg ggaggttact
     7501 gtacaggaac tatcggacac cataaccagt gcaataaaca aatattctgt agatgctggt
     7561 gccatgcagg gtgctattta ttcaagtgtt gtcccacaat gtaatgatgc ctatgatgca
     7621 gtatgtaaaa agctattttc tataaatccc cacaggataa cctaccgttc acgtatgcca
     7681 ataacaatag tatatgataa cccggcccaa ttaggtgttg atcgcattgt caatgccgtt
     7741 gctgcccata ttgattacaa caccgattgc attattgttg atattgggac tgccataaca
     7801 ttctgcctgc ttgaacatgg tgtattaaaa gggggaatca tagcacccgg ggtaaaggca
     7861 gcaatgaaag gcctggcaca gagcaccgca cagctttttg aggttccgtt tgaaaaacca
     7921 gcttctatta ttgcacaaaa tacaaaagat gcccttattt caggattttt ttacggatgg
     7981 atttctttaa ttgaagggat tatacaaaaa atggcaggtg cgatagctca ttatactgtt
     8041 atctgcactg gaggaatggc tgaagtgtta caaccctatc tttcaatccc tgcaaacatt
     8101 gacacaacac ttaccatgag agggttatat tacctgtacg acatcaacag gtaaaatgtt
     8161 gaatacccca aaaaacagtc tatttatatt aatattcaca aagttatcca cattatccac
     8221 aaatgcagca ggcaatgaca ttaatttact aaattaattg gctatcgatt gaatgtatgt
     8281 tactatgtcg cattgaactg catcgaatgc agtaaattta ttgaataaag ctatgctaaa
     8341 tgctgaaaat tccaggattt atgttgcaac attttttaaa aaaaaatttt tttactttta
     8401 taattattta tatgtataag ctgaaagtgc atatattcaa aaattaattt ctgttaaaat
     8461 tcattataat gtggataaaa gcttgaataa aaattgcatt atcataaata aaaatatgtt
     8521 gtatttccaa acattggtta atgaacgttc acatattttt tattagtttt tcgcaaacaa
     8581 tatcaaacca gaataatcct gggtatatca taaagaagtc atattggcct atgaagttca
     8641 atgacccttt ttatatatca cccatttaaa tttggttaga attaaaaaaa taatgtcttt
     8701 tctatatctt gtatcattac cccatccagt aatctataca gaatgatgca gattcacgtt
     8761 tgaagtgcac cattgagttt tccctgtaaa acctcatacg aagatttcca tcctttagat
     8821 tttcctggac gtgcatgtat cattatagtt ataccatcta ttccctcatc gcttacataa
     8881 gcaaaacttt ttttcttttg caataaatat tgatataacc tgttggtgtt ttccacaaca
     8941 ctccgttgcc aggcacaata cggatctgca caataaatat tacagtccag tatttcctct
     9001 cattttttgt gataggtaca ttctgtccca gtatcaaacg gtatggtttt ttcaattttt
     9061 cctgttaaac tttagatcac tatctctatc ccacgcataa cggtgtctgc agtctatgaa
     9121 actttagagc cttcccctgc actttctttt gtccagacgg caacaa
//
LOCUS       DAOSZP010000009         9133 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_6470_length_9133_cov_3.580414, whole genome shotgun sequence.
ACCESSION   DAOSZP010000009 DAOSZP010000000
VERSION     DAOSZP010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 9133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9133
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6470_length_9133_cov_3.580414"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..544
                     /locus_tag="PK348_00455"
     CDS             <1..544
                     /locus_tag="PK348_00455"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXK64714.1"
                     /translation="LDYAHAAAWANKAIDDKPNFARGCDVYACSMYQQQKYDEAVEYW
                     GRALQLGLDEDRVYYNRACAYFAMGNYNRCIADCTKALKKNKKNINALILLAFTHFTL
                     NDYDAALSACNNALKIQNNTIVAAVLRAVCAGKKNAQIGKDMLVATLGSVENVQDVAM
                     KLLQNFQYTIIDSPADFMLY"
     gene            complement(547..>982)
                     /locus_tag="PK348_00460"
     CDS             complement(547..>982)
                     /locus_tag="PK348_00460"
                     /inference="COORDINATES: protein motif:HMM:NF025743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4416 family protein"
                     /protein_id="HXK64715.1"
                     /translation="LQKKFGTIDLQSEPIPFSHTDYYSTIGKNLYKVFISFEKLINRE
                     DIAKIKLFTNKVETKFLHNGNRTINIDPGYLTLSNVFLASCKDYFHRVYVGKGVYVEN
                     EYRYVAKHYQPWEWTYPDYRKKEYLQFFETLRSIYYEQIKQR"
     assembly_gap    983..992
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1050..2030)
                     /locus_tag="PK348_00465"
     CDS             complement(1050..2030)
                     /locus_tag="PK348_00465"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HXK64716.1"
                     /translation="MEKDSITSTTTQKLISIVPDGYKPMRLDVYLAGRFTYLSRSRWQ
                     KEIKGGKIFVNNIPITIPHYIVYQHYVIEYKRQPVEEPTVDPHYTILYEDDYIIAVNK
                     SGNLPTHPAGKYFHNTLTMLLWSHTGIQCMPLNRLDRETSGVVVLAKSGRIASAFLQY
                     SKTIAKTYYAIVIGNCSFTRLTVDTPIGISQNSRIRKKRAAYTDAQWHAVTHFTVIKR
                     YNGYTLIKAVPQTGRTHQIRVHCEHIGHPIAGDTLYCNHDDDFLAYIQSQGTIQRPFP
                     RCALHSRMYRLYHPFLQKMIAIKAPLPQDMRTFLLQLESTNGTANNTTQS"
     gene            complement(2137..2598)
                     /locus_tag="PK348_00470"
     CDS             complement(2137..2598)
                     /locus_tag="PK348_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK64717.1"
                     /translation="MNLETTTCISYEHLDIIQRYAKLHKLSLSMFIINFINYVVSYKT
                     INTKAYSRLSYRTRYSGNWRRLHIVLLEHEYEFIMDVRKVYKMSLAKVIAYCIDNYLY
                     DFLNALEKEDNTDNYRVSGYSFLVFLEEGIQCCQFYWGPHPEILKIANATP"
     gene            2806..2922
                     /gene="rrf"
                     /locus_tag="PK348_00475"
     rRNA            2806..2922
                     /gene="rrf"
                     /locus_tag="PK348_00475"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(2996..3745)
                     /locus_tag="PK348_00480"
     CDS             complement(2996..3745)
                     /locus_tag="PK348_00480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK64718.1"
                     /translation="MIYKFFAFIILLFLPQTNLLGYSILPETIHSQKHYLKSTQEITL
                     TSTPAIRAFITQNIPITNNITLEAYYTFKKPQNIKNPMTGSNRLFIINILHSISSMQG
                     IKYYSQTRKRLRILFDKSYIVQYPSLKPLHDPCINKLIDEYSFYAMQKDTTFGEVIYQ
                     YTIYQKPEFILLKSENMDVMKYFMMPIVDKKGFKFFLFILVNGEDIALYCLYSIEVIN
                     DKVFPLQKVRDSLFNRSDAIVLWFYNRLFGK"
     gene            4260..5048
                     /locus_tag="PK348_00485"
     CDS             4260..5048
                     /locus_tag="PK348_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017436097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HXK64719.1"
                     /translation="MIKVYIVDAFTSEMFKGNPAAVCVLSSALPDALMQAIARELNCS
                     ETAFVLPHNNEFLLRWFAPLQEVDLCGHATLAASHVLYEQRIATAQQELRFHTKSGLL
                     SVKKQDEIITMDFPQEIATEYSIPLWVIEALGVKPVYTGKNRMDYLIEVETEKEVLEL
                     NPDFIALKKMDSRGAIITAKSSRKEFDFVSRFFCPGLGVNEDPVTGSAHCCLGPYWQQ
                     KLQKNTFNAYQASQRGGILRVEVGESRVYIGGKAVTVLSGLINI"
     gene            5204..6022
                     /locus_tag="PK348_00490"
     CDS             5204..6022
                     /locus_tag="PK348_00490"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HXK64720.1"
                     /translation="MPAIIVDKEKCKMDKLCVMECPMKIISVDDSGIPQTLNTAESMC
                     IECGHCVAVCPHGALSLPMLRAEECQDIHYQWNPGVTVIENYFKSRRSIRRFKKDIVK
                     KEDLMKLLEITAYAPTGHNSRTVEYIVYTQKEEIHALVKHVIDWMHTIIINSPEMAKS
                     MHFDMITKAWEDGIDTVTHSAPVIIVAHGKKSNPNTPTSCTIALTHLELIAPALGLGC
                     CWAGYFSWCAMVYHPLKKALQIPDGNTVYGTMLVGYPLVRYYRIPKREPCIEWR"
     gene            complement(6115..6567)
                     /locus_tag="PK348_00495"
     CDS             complement(6115..6567)
                     /locus_tag="PK348_00495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK64721.1"
                     /translation="MKKLLLVALIAVVAISFTACGSQKIGGQVKGETFISEGWVTPDI
                     FRVTATGVPKPGLKNKIQRRGTAKEAAIIMAEKRIIEKFVGARLEGAAGAADYASTGV
                     AVAKEFGGLVKGGTIVKETYDDDDNCEIVYQVEKKGLKKEVEGGAYER"
     gene            complement(6795..7331)
                     /locus_tag="PK348_00500"
     CDS             complement(6795..7331)
                     /locus_tag="PK348_00500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK64722.1"
                     /translation="MGLLSIMLLCIFYTILIVIVYEYYKLFNSKDEYTKQELKQVVFL
                     IPDHWIPLLSKFRLYPVYALTSLVFGILIPLLSTNWFFQSSVFCILLFIILPQIRRSY
                     EPMKVTVSDSFVDTVATALSEYYEIILFFFSTGTLSSLTYTWVTEKELSFLWFMVNAI
                     IICIIMFFMLASLDKENI"
     gene            complement(7629..8237)
                     /gene="purN"
                     /locus_tag="PK348_00505"
     CDS             complement(7629..8237)
                     /gene="purN"
                     /locus_tag="PK348_00505"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018249462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HXK64723.1"
                     /translation="MKKTVSFLVSGRGSNFQAVASAIIKGDIPATVGIVISDKPGVKA
                     LDIARELSIPAFFVNPKDYATRQDHEKAMIDLLQKCNTDLVVTAGYMRLLTPFFVNEF
                     KNRIINIHPALLPSFPGTHAQKQALEYGVKISGCTTHFIDEGTDSGPIILQKAVPVYD
                     DDTEDTLSARILKEEHQILIESVKLYCMDKLKVKGRKVFIVR"
     gene            complement(8315..>9133)
                     /locus_tag="PK348_00510"
     CDS             complement(8315..>9133)
                     /locus_tag="PK348_00510"
                     /inference="COORDINATES: protein motif:HMM:NF037782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK64724.1"
                     /translation="LPLVFYRCDDEYANVIAHPRHGYGKNANPCIDCKIFFLQKAKEY
                     MGKLNASFVITGEVVGQRPMSQYKSMLKHIEKECGLEGLLLRPLSAKLLPPTIPELEG
                     IVDRNRLYAIAGRSRDTQFELAQEFGITQFQSPAGGCFLTMPQLASRVKDLIEHPPFN
                     ALDLFLANVGRHYRLHEHAKIIVARNENECTILQRYKQTADFFVEPSFKGPVIYGKGT
                     ILQSDYPVLVQILARYGKPSPDENSVALFIRGHHTTTLVTDNPIDDATLSKMRI"
BASE COUNT         2841 a         1541 c         1769 g         2972 t
ORIGIN      
        1 cttagattat gcacatgcag cagcgtgggc aaacaaagcc atagacgaca agccaaactt
       61 tgcccgtgga tgtgatgtgt atgcatgcag catgtatcag cagcaaaaat atgatgaggc
      121 agtggaatac tggggaaggg cactgcaact ggggctggat gaagatagag tgtactacaa
      181 cagagcctgt gcctattttg caatgggcaa ttataatagg tgtattgctg attgtactaa
      241 agccttaaag aaaaacaaaa aaaatattaa tgctttaatt cttttagcat ttacacactt
      301 tactctgaac gattatgatg cagcgttgtc agcatgtaac aatgcgttga agatacaaaa
      361 taatactatt gttgctgctg tactcagggc agtgtgtgcc ggtaaaaaaa atgcacagat
      421 agggaaagat atgcttgttg caactcttgg cagtgtagag aatgtacaag atgttgctat
      481 gaagcttttg caaaattttc aatatacaat tattgattcg cctgccgatt ttatgttata
      541 ttaaaattac cgttgtttta tctgttcgta gtagattgac cgcagtgttt caaaaaattg
      601 aagatattct tttttacggt aatcgggata tgtccattcc cagggctggt aatgttttgc
      661 cacataccga tattcatttt ccacatacac gcctttgccc acatacacac gatgaaaata
      721 atccttgcat gatgccaaaa atacattgga aagggtaaga tagccagggt caatatttat
      781 ggtgcggttg ccattgtgta aaaacttagt ttctacttta ttggtaaata gttttatctt
      841 tgctatatct tctctattaa taagtttttc aaagcttatg aatactttgt ataaattttt
      901 cccaattgta ctgtagtaat ctgtatgtga aaagggtatt ggttctgatt gtagatctat
      961 ggtgccaaat tttttttgta atnnnnnnnn nnatattgat gctatgaaac aaaacatcat
     1021 cgcgggcggt taatagccct atgaacagtt tagctttggg tggtattgtt ggctgtgcca
     1081 ttggtgctct ctaattgtaa taaaaatgta cgcatatcct gtggcaatgg cgcttttatt
     1141 gcaatcatct tttgcaaaaa tggatgatat aagcggtaca tgcgcgaatg aagggcacag
     1201 cggggaaatg ggcgttgtat ggtgccttgt gattgtatgt atgcaaggaa atcgtcatca
     1261 tggttacagt acagtgtatc gccagctatt gggtggccta tgtgttcaca gtgtacgcgt
     1321 atctggtgag tacgccctgt ttgtggcact gctttgatga gtgtgtaacc attgtatcgt
     1381 ttaattacag tgaaatgggt aactgcgtgc cactgtgcat cggtatatgc tgcccgcttt
     1441 tttcggatac gtgagttctg tgatattcct atgggggtgt caactgttag cctggtaaag
     1501 gaacaattac cgataactat cgcataatat gtttttgcga tagttttaga gtattgtaaa
     1561 aatgctgagg caatgcgtcc gcttttagca agcacgacca cacctgatgt ttcgcggtct
     1621 aagcggttga gtggcataca ctgaatgcca gtgtgggacc acaatagcat ggtaagtgtg
     1681 ttgtggaaat atttaccagc agggtgagtg ggaagattgc cacttttgtt aactgcaata
     1741 atgtaatcat cttcgtagag tatggtgtaa tgggggtcaa cagttggttc ttcaacgggc
     1801 tggcgtttgt attcaataac atagtgctga tatacaatat aatgagggat agttatcggt
     1861 atattattaa caaagatttt accgcctttg atttcttttt gccaacgtga gcgggaaaga
     1921 taggtaaaac gtcctgccag gtaaacatcc aaccgcattg gcttgtatcc gtcaggaacg
     1981 atagagataa gtttttgtgt tgtggttgat gtaatgctgt ctttttccat agcttaatga
     2041 tgcacgtgca taccagtatt gtaattaaat atacttcatt gttgtgtgta aacaagacaa
     2101 atttgcgtat agcttatatg gctagtataa aaaagtttat ggtgtggcat ttgctatttt
     2161 taatatttca ggatgtggtc cccaataaaa ctggcagcat tgaattccct cttcaaggaa
     2221 aacaagaaaa ctataaccac tgactcgata gttatcggta ttatcttctt tttcaagggc
     2281 attgagaaag tcataaagat aattatcaat gcagtatgca ataacctttg ccagagacat
     2341 tttgtaaacc ttcctgacat ccataataaa ttcatattca tgttccagta acacaatgtg
     2401 aagccttcgc caattacctg agtagcgtgt tctgtaagac aagcgtgaat atgcttttgt
     2461 attgatggtt ttatatgaaa caacatagtt aataaaattt ataataaaca tactcaaaga
     2521 cagtttatgt agttttgcat aacgctgaat gatgtcaaga tgctcgtatg aaatgcatgt
     2581 ggtggtttcg agattcatag tatatcctcc caaagtaatg ctattttttt aagattaaac
     2641 agtaaatttt tgattcaaaa tttcaacttt tttttgaaca acaacggttt tgcgataatc
     2701 ttataaaaga ttttattgtt aaatttttta aaaattttac ataaattcta ttgacaaagt
     2761 ttgactatct ttttataagt ccattcacac ggtaagtaaa ttcttcccgg tggttataga
     2821 gagggggaaa cacctgttcc cattccgaac acagaagtta agcccctcat cgccgatgat
     2881 actgcaccgg taacggtgtg ggaaagtagg tcgctgccgg gattgcaatc ataaatatta
     2941 ttataaaaaa gccactctgt aatgagtggt tttttttatt gatactatta acgtattatt
     3001 tcccgaaaag cctattgtaa aaccataaaa cgatagcatc ggaacggttg aacaatgaat
     3061 cccttacctt ttgcaaagga aatactttat cgtttattac ttcaattgaa tataagcagt
     3121 ataaggcaat atcttctcca ttgacaagaa taaacaaaaa gaatttaaat ccttttttat
     3181 ccacaatagg catcataaaa tatttcatta catccatgtt ttcagatttt aacaggataa
     3241 attctggttt ctggtatatc gtatattgat aaattacctc accaaatgtt gtatcttttt
     3301 gcattgcata aaaagaatat tcatctatca atttgttaat acaaggatca tgtaatggtt
     3361 ttaatgaagg atattgtaca atataagatt tgtcaaaaag aatgcgtaat ctctttcttg
     3421 tttgtgaata gtatttaata ccttgcattg agctaattga atgtaaaata tttattataa
     3481 acaatctatt gctgccggtc attgggtttt ttatattctg cggtttttta aacgtataat
     3541 atgcttccag tgtaatgtta ttcgtaatag gaatattttg agtaataaac gctcttattg
     3601 caggtgtaga tgtaagtgta atctcttgtg ttgatttaag ataatgtttt tgactatgta
     3661 ttgtttccgg aagaattgaa taccccaata aatttgtttg aggcaaaaaa agcaatataa
     3721 taaatgcaaa aaatttatat atcatatggt ggttgcagtc ccttataaaa tagttgtgaa
     3781 tatatcctgt ataatatata tgtataaatg tatctttgat ttatatagat agcaaatatt
     3841 ttccaataca agaagagtgt aattttttca gtgatataat gagttaatac tgttatattt
     3901 aagcttatat caaaaggtat tcaataagtg aaacagcata ctatttactt tgcatttact
     3961 tttttatggt catggagttt atggtctatc ccaatttttt tgagtttgca agttacccat
     4021 cctgtaagta tgttattata tatcggtggc ggcatagcac cttcttcaac ggggattatt
     4081 cttgcaaggc tcaggggcga tagttactgg aaaagtttta taaggtgttc tatttcattt
     4141 ttgtataaat ttttttagtg aaataataga catttccaac gaaacaaaat atttcaggac
     4201 tgtagtgcaa ttaatgattg ctataataat tttacttact aggaaaaagg agctaaagaa
     4261 tgataaaagt atatatagtt gatgccttta catctgaaat gtttaaagga aatcctgctg
     4321 cagtatgtgt attgtcttct gctcttcctg atgcattaat gcaggcaata gcacgtgaat
     4381 tgaattgctc tgaaactgca tttgtattgc cgcacaataa tgaattttta ctcaggtggt
     4441 ttgctccgct tcaagaggtt gatttgtgtg gtcatgccac attggctgca tctcacgtgc
     4501 tttatgaaca gcgcatcgcg acagcacaac aagagctaag gtttcatacc aaaagcggtt
     4561 tgctttcggt aaaaaaacaa gacgaaataa ttacgatgga ctttcctcag gagatagcca
     4621 cagagtattc aattcctcta tgggttattg aagcattggg tgttaaacct gtgtacacag
     4681 ggaaaaatag gatggattac cttattgaag ttgaaacaga gaaagaggtt ttagagctca
     4741 atcctgactt tatagcctta aagaaaatgg atagtagagg tgccataata acagcaaaat
     4801 cttccagaaa ggaatttgac tttgtatcaa gatttttttg tcctggtctt ggtgtaaatg
     4861 aagatcccgt tactggatct gcacattgct gcctgggtcc ttactggcaa caaaaactac
     4921 aaaagaatac ttttaacgcg taccaggctt cacagcgtgg gggtattcta agggttgaag
     4981 tgggtgaatc acgggtctat attggcggga aggcagtcac agtgttaagt ggcctcatta
     5041 atatttaaca atttttagat aaccacatgt aagcacctgt aattgaatgt tggataaatg
     5101 tgtatatcat acatagcctt tatatgcaaa atggaagttg ttgtatttga tatttgtata
     5161 gatagtctag ttactataat cttcatgttc aaaggagtgt ttaatgcctg caataattgt
     5221 agataaagaa aaatgcaaaa tggacaaatt gtgtgttatg gaatgtccaa tgaagataat
     5281 ttcggttgat gatagtggta ttccacagac acttaatact gccgagtcta tgtgtattga
     5341 atgtggacat tgtgttgcag tttgccctca cggtgcattg agtctaccaa tgctaagggc
     5401 tgaagagtgc caggatattc attaccaatg gaatccgggt gttactgtaa tagaaaatta
     5461 tttcaagtca aggcgttcaa tacgcagatt taaaaaagat atagttaaaa aggaagatct
     5521 gatgaaactt ctggaaataa ccgcctatgc cccaacaggc cataattcac gcactgttga
     5581 atatattgtg tatactcaaa aagaagaaat acatgcattg gtcaaacatg taattgattg
     5641 gatgcatacg ataataatta attcccctga aatggcaaag agcatgcatt ttgacatgat
     5701 aacaaaagcc tgggaagatg gtatagatac agtaactcac agtgcgccag taataatagt
     5761 tgctcatggc aaaaaatcca atccaaatac tccaacatca tgcactatag cccttacgca
     5821 tttagaatta attgccccgg cattagggct tggttgttgt tgggcaggat acttttcatg
     5881 gtgcgcaatg gtatatcatc ccttaaagaa agctttacaa atcccagatg gtaatactgt
     5941 gtacggaaca atgctggtgg ggtatccgtt ggttagatac tatcgcattc ctaaacgaga
     6001 gccttgcatt gaatggcgat aaaataacct tcttaaaaat tattaaaata tttatgcaaa
     6061 ataaaaaagc aggcgactca acgcctgctt taattttact ttgatgaaat tgaattatcg
     6121 ctcgtaagct ccgccttcaa cttctttctt taaacctttt ttctcaacct gatatacgat
     6181 ttcacagtta tcgtcatcgt cataggtttc tttaacgatg gttccacctt ttacaaggcc
     6241 accaaattct tttgcaactg caacaccggt agatgcataa tcagcagctc ctgcagcacc
     6301 ttccaaacgg gcaccaacaa acttttcaat tattcgcttt tcagccataa taatagctgc
     6361 ttcttttgct gttccgcgtc gctggatttt attttttaat cctggttttg gtactcctgt
     6421 ggcagttact ctgaaaatat cgggtgttac ccatccttcg gatataaacg tttcaccttt
     6481 tacctgacca cctatctttt gtgatccgca tgctgtaaag ctaattgcta ccacagcaat
     6541 taatgcaact aataaaagct ttttcataaa cagctcctcc ttctctttag gggggttacc
     6601 ccatattata ttattaataa actaagtgag tatagggaca taccatatac ttaaagcaaa
     6661 tagtaataaa aataaaaata aacgtcaaca taaaaaataa aatttttagg tattaaaccc
     6721 ctgtactcct cttagtatat tattcaaata atatatgata gtacaaatat tttgtataat
     6781 agtgcaaaaa aaatttatat gttttcctta tccagcgaag ccagcatgaa aaacatgatg
     6841 atacatataa taatagcatt aaccataaac cataaaaatg aaagctcttt ttcagtaacc
     6901 caggtatatg tcagtgatga caaagtacca gttgaaaaga agaataaaat aatttcatag
     6961 tattcagaaa gggctgtagc taccgtatca acaaatgaat cagataccgt aaccttcatt
     7021 ggttcgtacg acctgcgtat ctgtgggagt ataatgaata aaagaatgca gaatacggat
     7081 gactggaaaa accagttagt tgatagtaac gggattaata tgccaaacac cagagaggtc
     7141 agtgcatata ccgggtataa tctgaatttg gataatagtg gtatccagtg atctggaata
     7201 aggaaaacta cctgttttaa ttcctgtttt gtatattcat cctttgaatt aaaaagttta
     7261 tagtattcat atacaataac aatgaggatt gtataaaaaa tacaaagtaa cattatagaa
     7321 agtaacccca tacagttcct ccaaaaatat agtttattta gcataattta agatgccagc
     7381 ttctgtcatg acaatcttgt cggcatgcct gcataatcat gaactgcatt acctggaatc
     7441 aattaaaaat ctgctatctt gaatcttgct aaacacagta ccatctaagt tcacaaagtc
     7501 actctgaata tccttaattg ttttaaaaat tatattcagg aatactcact ctatactgta
     7561 tagtgaaatt ttatgcaagt attttattta taaaggatac gtacttgcaa cgataccatt
     7621 aacagtaact atcgcacaat aaaaactttt cttcctttca ctttaagttt atccatacag
     7681 taaagcttta ctgattcaat cagtatttga tgctcttcct tgagaatacg tgctgagagt
     7741 gtgtcttcgg tatcatcgtc atatactggc acagcttttt gcaagataat tggccccgag
     7801 tctgttccct catcaatgaa atgcgtggtg catccagaaa ttttaacacc atactcaagt
     7861 gcctgcttct gcgcatgagt accagggaat gatgggagta gtgcagggtg tatattaata
     7921 atcctgtttt taaactcgtt tacaaaaaat ggagttaaaa ggcgcatata gcctgctgtt
     7981 accaccagat cggtgttgca tttttgtaaa agatcaatca tggctttttc atggtcctgg
     8041 cgcgtggcat aatcttttgg attaacaaag aaagctggta ttgaaagttc acgtgcaatg
     8101 tccagagctt ttacacctgg cttatccgaa ataacaatgc ccactgttgc aggaatatca
     8161 cctttgataa ttgcactggc aacagcctgg aaatttgacc cacgtcctga tactaaaaat
     8221 gatactgttt ttttcatatg ccctctcatg atattctcta taatctggtt gtgataaaca
     8281 catgcagcat ataccgcttc ttatcctggt atgattagat acgcatcttt gataatgttg
     8341 catcgtcaat tggattatct gtaactaatg ttgtagtgtg gtgcccccgt ataaataatg
     8401 caacactgtt ttcatctggt gatggtttgc catatcgtgc cagtatctgt acaagtaccg
     8461 ggtagtcact ttgcaggata gttcctttgc catagatgac gggcccttta aatgatggtt
     8521 ccacaaaaaa gtcggcggtt tgtttgtagc gctgcagtat ggtgcattcg ttttcattac
     8581 gtgctactat tatttttgca tgttcatgta agcgatagtg cctgccaaca ttggccagaa
     8641 aaaggtctaa tgcattaaat ggcggatgtt ctattaagtc cttaacacgt gaagcaagct
     8701 ggggcatggt taaaaaacat ccacctgctg gtgattgaaa ctgtgttata ccaaattctt
     8761 gagctaattc aaattgcgta tcgcgcgaac gcccggcaat ggcatagagg cggtttctat
     8821 caacaatacc ttccagttca ggtatggtgg gtggtaaaag ttttgcggat agtggtcgca
     8881 gcaagaggcc ttcaagtcca cattcttttt ctatatgttt gagcattgat ttatactgtg
     8941 acatggggcg ctgaccaaca acctctcctg taataacaaa ggaagcatta agctttccca
     9001 tgtattcttt agctttctgt aaaaagaaga ttttgcaatc aatacagggg ttagcgtttt
     9061 taccgtatcc atgtctggga tgcgcaataa catttgcata ttcatcatca catcgataaa
     9121 acacaagagg aag
//
LOCUS       DAOSZP010000010         9055 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_6540_length_9055_cov_2.968778, whole genome shotgun sequence.
ACCESSION   DAOSZP010000010 DAOSZP010000000
VERSION     DAOSZP010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 9055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9055
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6540_length_9055_cov_2.968778"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            153..914
                     /locus_tag="PK348_00515"
     CDS             153..914
                     /locus_tag="PK348_00515"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HXK64725.1"
                     /translation="MAQTIQSLDRGLKILEILGKSDKPLSLNEITEYFDIDRSSIFRL
                     LGTLIAHNFVHQDPDTKKYMLGFKIMELSGAFGEQTKIETFLRPVLKYVCEKTQQNTH
                     LAMLHGQDVVFIAVEQPRNSLSMHISVGTREPAYATALGKVLLAFMPQPEKEMLLREV
                     TLQKYTSKTITSVAVYKKHLEAIVNTKYAIDNEEYRLGVVCIAAPVFNHRGVVEYSIG
                     ISGSREQVCPKLDEYISVVKDAGAEASRLLGMPSQ"
     gene            complement(917..1372)
                     /locus_tag="PK348_00520"
     CDS             complement(917..1372)
                     /locus_tag="PK348_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK64726.1"
                     /translation="MRILIYCAILFIGFTSCQPSKPLTPQEAFNALKDAYTHNNMQNG
                     AALLSTNSKKRISTVIHNLQSMNKAQLSAFAQYYGISSDSIKTMDIPEYLLLQKKIAA
                     TRGDDVLLTALSQPIAQVTIAQSKATVQLYNGMSLIFVKEGPYWYFEYE"
     gene            complement(1360..2229)
                     /gene="panC"
                     /locus_tag="PK348_00525"
     CDS             complement(1360..2229)
                     /gene="panC"
                     /locus_tag="PK348_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HXK64727.1"
                     /translation="MKIVNTINELRNEIALQRHHNKKIGFVPTMGYLHQGHLSLVHIS
                     KQHSDYQVMSIFVNKMQFNDPKDYTTYPRDYERDFALAKEAGVDLVFLPDDSEMYQNR
                     LVYVDIEMMTDHLCGATRPGHFRGVLTVVAKLFNIVQPDVSVFGQKDIQQAVIIQKMV
                     KDLDFPVTIIVAPIIREPDGLAMSSRNVHLKGDDRTNAVVIHKSLQKAEELIHQGVVQ
                     SHKLIPAMTDIIASGKPKKIDYVSVVDYETLQPIETLKEKSVIAVAAFFGPTRLIDNM
                     IVEKKGDTYTCVY"
     gene            complement(2233..3087)
                     /locus_tag="PK348_00530"
     CDS             complement(2233..3087)
                     /locus_tag="PK348_00530"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXK64728.1"
                     /translation="MSYETASCVLCNTYNFKPLFSKPFENGEEFTLVRCRHCGLEFVN
                     PRPYYNDMSRYYKEYFNQRTDRGYNNYFSEAMHREVLRLLTLNLQDLLFYDFEASLPG
                     EKYALDIGCAAGYFVDYLTHRGWHAEGIDIAQDCIEYAHNTLKVNAYCGDFLSFHFNK
                     QYHCITLWATIEHLHYPDKFIEKIHTLLHDEGMLYLSTCRSSAFSARLLYGKKWRYYN
                     FPHHLYFFSYRTLAKLLQLKGFAVTRFVTYGSGIGKPKTLVRKLADSAAKRLYLGDMM
                     LIAARKEK"
     gene            3264..4340
                     /locus_tag="PK348_00535"
     CDS             3264..4340
                     /locus_tag="PK348_00535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK64729.1"
                     /translation="MSVVLYYNTNKTMKVSNRIIVITQNKDWEAIASAIASQHQLIIE
                     ENEKLHSLEAIIKEIENSKSTAFLRSSLHSFIREHGIPFLIIIDYPVVAETRTDAIVQ
                     KIFTTLLISFMIIARGSGLANIKGNFFVNITRGDAQLFKHSIIHPEKLLAKLKTNDEK
                     VNAIINLYADQKIFHTLFFVKPCTGATKEDMAHELLAYIDAVKKRHELIEKIVEKQKH
                     TSSRSKDAATVLVKISEDKIVLDHEIMITRDSAYKKYTAGHIYVLGDWTNIHSRKVAG
                     KVITAIKDGFADWKLGSDDPVIIHLEDALVDHTTAATLAQIAFSELRGFSNIRIYCNE
                     KNYKILEAADGFGLIKKLVFIQKS"
     gene            complement(4337..7774)
                     /gene="dnaE"
                     /locus_tag="PK348_00540"
     CDS             complement(4337..7774)
                     /gene="dnaE"
                     /locus_tag="PK348_00540"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF005298.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HXK64730.1"
                     /translation="MDFVHLHNHSEYSILDGAIRIQQLIKFAVDNHMPAIALTDHGNM
                     FGMIEFYEEAHKAGIKPIIGQEFYIAPGSRFHKQSTRASNEENAYHLILLAENYEGYK
                     NLIKLSSLGYIEGFYYKPRIDWELLTRYSKGLIASTACIAGEIPSLILEGKVKEAYTR
                     AQEFADLFGKDHFYLELQYHNLKEQKQVNAHLVEMASKLNIGLIATNDAHYVARDDAY
                     YHDVLLCIQTGKLLLDQGRMRFPNDQFYLKTPQEMEKLFEDYPDALYNTMKIAEMIDC
                     QIPLGNPILPNFEVPAGYTKDSYLTELVYEGAKKRFGGTIPDYVKERIDYELSVIQRM
                     QFSGYFLIVWDFIQFAKNNGIPVGPGRGSAAGSMVSYCLGITELDPIKYNLLFERFLN
                     PERNEMPDMDIDFCALRRDEVIEYVKKKYGEDHVSQIITFNKMKSKGAIRDVARALNI
                     PLPQVNALTKLIHHEDLEEALKNSEELQQLYKSNDAGKQLIDIALKVEGLTRSAGKHA
                     AGVVISRDPLTEHVPLYVDSKDGSISSQYEKTSLEKAGLVKMDFLGLANLTIIDTCIK
                     LIKKHRNIDIDINAIPLDDAKTFKLLQKADTMGVFQLESGGMQNILLKLGPTNFDDII
                     AIVALYRPGPLDSGMVEQFIERKRNPQKIEYLHPSLEPILKDTLGVIVYQEQVMLISQ
                     IMGGFTLPEADKLRKAMSKKKQDLIDAMSSKFVKGAIAKGIHEEIACKTYELMATFGR
                     YGFNKSHSAAYALVSYQTAYLKAHYPLEFMTALLTAQVDKIEDIAKYYADCKAKGIQV
                     LPPDINKSQRDFSIEGKSIRYGFIAIKGVGDKAIESILQARENKGMFKDLTDFFTSID
                     LMTVNKGVLESLIKAGAFDSLHPNRAALFTSVDVMLETARQLQQDIATGQGDLFGATD
                     TSFSRIHIEPASVNDWPENQKLQYEKEVLGIYASSHPLSRYAKDIEKLSCTPIKDLEE
                     NVNGTPITLVGILNNVQIKQSQKGNKFAQAVIEDLTGSIQVLFMPQTLSEYEPLIVSQ
                     EPVVITGTVEIENEKPSRMLAQKVLQLTQFKLTQISELHIIINPIGTDTQLLENIKKL
                     LQRYQGNKPVFFHVRQSNGKENIVKANPLYHIKPHQKLLDSLVSIVGKDGFFYTIGC"
     gene            8203..8364
                     /locus_tag="PK348_00545"
     CDS             8203..8364
                     /locus_tag="PK348_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HXK64731.1"
                     /translation="MIDTKLLDILACPACKGDVEYDARNEKILCTQCKKKYPVKDGIP
                     VMLVEEAEE"
     gene            8392..>9055
                     /locus_tag="PK348_00550"
     CDS             8392..>9055
                     /locus_tag="PK348_00550"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HXK64732.1"
                     /translation="MFDSKHPVIKIVGYVIVGFFVLIIIISFGMPDFLSRMGFDQNTI
                     AKVNGETIGYMDFIRYRDTHMFGKTEDPKQQQRMIIERMIQERLLVQLAKKEGIVVTE
                     KEIKNVIRNRFSDNTGTFNETFFKNFLDRFHMGISDYYKYVQQEIYLGKLQNLLLAGV
                     SVSPLELVSDFRIQNTKIKIQYAFVSNQELAKRYAKEITVTDEEVDAEMKKNPKELKD
                     PKT"
BASE COUNT         2750 a         1664 c         1782 g         2859 t
ORIGIN      
        1 attgtattat tgtcaataag aatttaatta attttttact gaatagttca ttcttagtaa
       61 tgcagaaaat gaaataatgt tgaaataata taattgttga cttttttgtc gttactgttg
      121 ttgcataata cgaaacacga ttgggtatat ttatggcaca aacaatacag tcactggaca
      181 gaggattaaa gattctggaa atactgggaa aatcagataa accactttcc ctgaatgaaa
      241 taacagagta ttttgatatt gaccgttcat caattttccg gcttttgggg acgctgattg
      301 cacataattt tgttcatcag gacccagata caaagaagta tatgctgggt ttcaagatca
      361 tggaactttc cggtgctttt ggcgaacaga caaaaataga aacattttta cggcctgttc
      421 ttaaatatgt ttgtgaaaaa acccagcaaa atacgcacct ggctatgctg catggccagg
      481 atgttgtatt tattgctgtt gaacagccac gaaatagcct cagtatgcat atatcagtag
      541 gaacacgtga gcctgcttat gccactgcac tggggaaggt gcttcttgct tttatgccac
      601 aaccagaaaa ggaaatgctt ttgcgtgaag taacattgca gaaatataca tcaaagacca
      661 taacatcagt tgctgtttac aaaaaacatc ttgaagctat agttaatact aaatacgcaa
      721 tcgataatga agaatacagg ctgggtgttg tatgtattgc tgcgccagtg ttcaatcata
      781 gaggtgtggt tgaatattca attggcatat caggttcacg ggagcaagtt tgtcctaaat
      841 tagatgaata tatttcagtg gttaaagatg ctggtgctga agcatcgcgg ctgcttggta
      901 tgccttcgca gtaatattat tcatattcaa aataccagta aggtccttcc ttgacaaaaa
      961 tcaaactcat gccgttgtat agttgtacag tggcttttga ttgggcgata gttacctgtg
     1021 caatgggctg actgagtgca gtcaaaagaa catcatcccc acgggttgct gcaattttct
     1081 tttgcaataa gagatattca ggtatatcca tagttttgat gctatcagag cttatgccat
     1141 agtactgtgc aaatgctgat aactgtgctt tattcattga ttgcagatta tgaataactg
     1201 tagaaatcct ttttttgctg ttagtggata acaatgccgc accgttttgc atattgttat
     1261 gtgtataagc atctttgaga gcattaaatg cttcctgcgg agtaagtggt tttgatggct
     1321 ggcaggaagt aaaacctata aacagaatag cacaatatat caatatacgc atgtgtatgt
     1381 atctcccttt ttttcaacga tcatgttgtc tattaaccgt gtaggtccaa aaaatgcagc
     1441 tacagctata accgattttt ctttgagtgt ttcaataggc tgaagtgttt cataatcaac
     1501 tactgaaacg taatcaatct tctttggctt acctgaagca ataatgtcag tcattgccgg
     1561 tatcagttta tgtgattgca ctacaccctg atgtataagt tcttctgctt tttgaagcga
     1621 tttatgtata acaacagcgt tagtgcggtc atcgcctttg aggtgcacat tgcgtgaact
     1681 cattgccagg ccgtcaggtt cgcggataat tggtgcaacg attatagtaa caggaaaatc
     1741 taagtcctta accattttct gtattataac agcctgctgt atgtccttct gcccaaatac
     1801 tgatacatcc ggctgaacaa tgttaaaaag ctttgctaca acggtaagca ctcctctgaa
     1861 atgtccaggg cgtgttgcgc cacacaaatg gtcagtcatc atttcaatgt caacatacac
     1921 caggcgattt tgatacatct cactatcatc aggaagaaat acaaggtcaa caccagcttc
     1981 ctttgccagg gcaaagtcac gctcataatc ccgtgggtat gtggtataat ctttgggatc
     2041 attaaattgc attttattca caaagatgct cattacctga taatcagaat gttgttttga
     2101 aatgtgtacc aggcttaagt gtccctggtg aaggtatccc atggtgggta caaatcctat
     2161 ttttttgttg tgatgtcgtt gtaaagcaat ctcgttacgt aattcgttaa ttgtattcac
     2221 tattttcata gtttatttct ccttccgtgc tgcaatgagc atcatgtccc ctaaatataa
     2281 cctttttgct gcggaatcag caagttttcg taccagtgtt tttggtttac caatgccact
     2341 gccgtatgta acaaaccgtg taacggcaaa cccttttaat tgcaataatt ttgcaagtgt
     2401 tctgtatgaa aaaaaataaa gatgatgggg gaaattgtag tatcgccatt ttttgccata
     2461 cagtagccgt gctgaaaatg cactgcttct acaggttgaa agatacagca ttccctcatc
     2521 gtgaagcaat gtatggattt tttcaataaa tttatcaggg tagtgcagat gctcaatagt
     2581 tgcccacagg gtaatacagt ggtattgttt gttaaaatga aacgatagaa agtcaccaca
     2641 gtatgcattt actttcagag tattgtgtgc atactctatg caatcctgtg caatatctat
     2701 accttctgca tgccatccgc ggtgtgttaa ataatcaaca aagtaccctg cagcgcagcc
     2761 aatatctaac gcatattttt cacccggtaa tgatgcctca aaatcataaa acagtagatc
     2821 ttgaagattc agtgtgagca gacgcagtac ttccctgtgc attgcttcag aaaaatagtt
     2881 attgtagcca cggtcggttc gctgattgaa atattctttg tagtatctgc tcatgtcatt
     2941 ataatatggt ctgggattga caaattcaag gccacagtgc ctgcaacgca ccagtgtaaa
     3001 ttcttcgcca ttttcaaatg gctttgaaaa taatggcttg aaattatatg tattgcatag
     3061 aacacaggag gcagtttcgt aactcatgta ttaattgtcg caggcattat atgcatttgg
     3121 caagtatttt ttatcatata aacagtaatt gattcctgaa gttttacaat agtcttgact
     3181 ttatatgatt aatagtatag ggttattaac aaatgtattc ttacgacaaa caatgtcaca
     3241 aaaatttaga atatgatgac tgagtgagcg ttgtattata ttacaataca aataaaacca
     3301 tgaaagtttc caatagaata attgttatca cacagaataa agactgggag gccatagctt
     3361 ctgcaattgc ctcccagcat caattgatta ttgaagaaaa tgaaaaactt cattcacttg
     3421 aagcaatcat aaaagaaata gaaaattcca aatcaacagc atttttacgc tcttcacttc
     3481 attcatttat ccgggaacat ggtatcccat ttttgataat aattgattac ccagtagttg
     3541 ctgaaacacg cacagacgct attgtgcaaa agatttttac cacattactc atatcattta
     3601 tgattattgc ccgtggcagt ggacttgcaa atattaaagg taattttttt gtcaatatca
     3661 ccagagggga cgcacaatta ttcaaacata gtataatcca tccggaaaag cttttggcaa
     3721 agttaaaaac aaatgatgaa aaagtaaatg caattattaa cctgtatgct gatcaaaaaa
     3781 tatttcatac tctgtttttt gttaagccat gcactggtgc caccaaagag gatatggcac
     3841 atgaactatt ggcctatatt gatgccgtta aaaagcgtca tgaactaata gaaaagattg
     3901 tagaaaagca aaaacatact tcttcacgaa gtaaggatgc agcaacagta ctggtaaaaa
     3961 taagtgaaga taagattgtg ctggatcatg agattatgat aacacgcgat agtgcctata
     4021 aaaaatatac tgcaggccat atttatgtac tgggagactg gacaaatata catagcagaa
     4081 aagttgccgg gaaagttatc actgcaatca aggatggttt tgccgactgg aagcttggca
     4141 gcgatgaccc tgttatcatt catcttgagg atgcactggt tgaccatacc acagctgcaa
     4201 cgctggcaca aattgctttt agtgaattga ggggcttcag taatataagg atatactgca
     4261 atgaaaaaaa ttataaaata cttgaagcgg ctgatgggtt tgggcttata aaaaaactag
     4321 tatttataca aaaatcttag cagccaatag tataaaaaaa tccatctttt cccacaatgc
     4381 ttactaagct atccagtagc ttctggtgtg gcttgatgtg ataaagcggg ttagctttta
     4441 caatattttc cttaccattg gactggcgca catggaaaaa taccggcttg tttccctgat
     4501 aacgctgcag taatttttta atattttcca gtagctgcgt atcggtacct atagggttaa
     4561 taataatgtg caattcgctt atctgggtaa gtttgaattg cgtaagctgc aatacctttt
     4621 gtgccagcat acgggatggc ttttcatttt ctatctccac agttcctgta ataacaacag
     4681 gctcctgtga cacaataagg ggttcatatt cagacaatgt ctgaggcatg aatagaacct
     4741 gaatgctgcc ggtaaggtcc tctatgacag cctgtgcaaa tttattccct ttttgactct
     4801 gcttaatctg tacattattg agaatcccaa caagcgttat gggtgttcca ttgacgtttt
     4861 cttccaggtc ttttattggt gtgcacgata acttttcaat atccttagca taacggctta
     4921 acgggtgtga actggcataa attcccaata cctctttttc atattgtaac ttctggttct
     4981 caggccaatc gttaactgat gcaggttcaa tatggatgcg cgaaaatgat gtatcggtag
     5041 ctccaaataa atctccctga cctgtcgcaa tatcctgttg caactggcgg gcggtttcaa
     5101 gcataacatc cactgaagta aacaatgctg cacggtttgg gtgtagcgaa tcaaatgctc
     5161 cagcttttat gagtgattca agtacaccct tgttcactgt cataagatca attgaggtaa
     5221 agaagtcggt gaggtctttg aacatgcctt tgttttcacg tgcttgtagt attgattcta
     5281 ttgctttatc accaacacct ttaatggcaa taaacccata gcgtatggat ttcccttcaa
     5341 tggaaaaatc acgctggctt ttattgatgt caggtggaag cacctgaatg ccctttgctt
     5401 tgcagtcggc ataatatttt gcaatatcct caatcttatc aacctgcgct gttaataggg
     5461 cagtcataaa ttctaacgga tagtgagctt taagataagc cgtttgatat gatacaagtg
     5521 catacgcagc agaatgggat ttgttaaatc catatcttcc aaatgttgcc attaactcat
     5581 acgttttaca ggcaatctct tcatgtatac cctttgctat tgctccctta acaaattttg
     5641 agctcattgc atcaatcaaa tcctgtttct ttttactcat tgctttcctg agcttatcag
     5701 cttcaggcag cgtaaaacca cccataatct gggatataag cataacctgt tcctggtata
     5761 caataacccc caatgtatct ttgagtatgg gttcaagaga ggggtggagg tattctattt
     5821 tctgaggatt gcgttttctt tcaatgaact gttcaaccat acccgagtca agagggcccg
     5881 gccggtaaag agcaacgatt gcaataatat catcaaaatt ggttgggcct aatttaagga
     5941 gtatattctg catgccccct gattcaagct ggaatacacc catggtatca gccttttgta
     6001 acagcttgaa ggtttttgca tcatcaagag gaattgcatt aatatcaata tcaatattac
     6061 gatgtttctt tattagcttg atgcatgtat caatgatggt aaggtttgca agccctaaaa
     6121 agtccatctt gacaaggccg gctttttcta agctggtctt ttcatactga gatgaaatac
     6181 ttccgtcttt tgaatccaca tataggggta catgttcggt taagggatca cgagatataa
     6241 ccacacccgc ggcgtgttta ccggctgaac gagtcagccc ttcaactttt aacgctatat
     6301 caataagctg tttacctgcg tcatttgatt tatagagttg ttgcaattct tcagaatttt
     6361 taagagcttc ttccagatct tcatgatgaa taagcttggt cagtgcattc acctgaggaa
     6421 gggggatgtt taatgcacgt gctacatcac gaatggcgcc tttggatttc attttattaa
     6481 aggttattat ctggcttacg tggtcctcgc catatttctt ttttacatac tctattactt
     6541 catcacggcg cagagcacag aagtcaatat ccatatcagg catttcgttt cgctcggggt
     6601 ttaaaaaccg ctcaaatagc aggttgtatt taatgggatc aagctcagta atccccaggc
     6661 aatatgaaac cattgaacca gcagccgaac ctctacccgg gccaacgggt attccattat
     6721 tttttgcaaa ctggataaaa tcccacacaa tgagaaaata gccagaaaat tgcatgcgtt
     6781 gtataaccga tagttcataa tcaatgcgct ctttgacgta atcagggatg gtgccgccaa
     6841 atcgcttctt agcaccctca tacaccagct cagtaagata gctgtcctta gtgtatccag
     6901 ctgggacttc aaaattggga aggatagggt tgcccagtgg tatctggcaa tctatcattt
     6961 cggctatctt catggtgtta tacagagcat ctgggtagtc ttcaaatagt ttttccattt
     7021 cctgaggagt tttgagataa aactggtcat tggggaaacg cattctgccc tgatcaagta
     7081 acaatttacc agtttgtatg cagagtaaca catcatggta ataggcatca tctcttgcta
     7141 cataatgtgc atcattggtg gctattaaac ctatattcag tttagaagcc atttcaacaa
     7201 gatgtgcatt tacctgtttt tgttctttaa ggttatgata ctgaagttct aaataaaaat
     7261 ggtccttccc aaaaaggtca gcaaattcct gtgcccgtgt gtaggcctct tttactttgc
     7321 cttctaaaat aagtgatggt atttcgccag ctatgcaagc ggtgcttgct ataagccctt
     7381 tactgtatct ggtcagcaac tcccagtcaa tacgtggctt gtaataaaag ccttctatat
     7441 agcccagtga ggataatttt ataagatttt tatatccttc ataattttca gcaagtaaaa
     7501 tgaggtgata ggcattttct tcattacttg cgcgggtgct ttgcttatga aagcgtgatc
     7561 ctggcgcaat ataaaattcc tgtccaatta ttggctttat gccagcttta tgtgcttcct
     7621 cataaaattc aatcatgcca aacatattgc catggtctgt taatgctatg gccggcatat
     7681 gattatcaac agcaaacttt atcagttgct gaatacgtat agcaccatcc aggatagaat
     7741 attcagaatg gttatgaaga tgtacaaaat ccatactgtt ctcgctttaa ggaagggatt
     7801 attgccatga gctatcgctc atgaaatacg acataataaa tatttggtat gtaaccaata
     7861 tactccctgt attttgattt tgcaagcaag tattaatatt ttttatactt tttgcaatat
     7921 gttgttgacc gtaatacaga tgtgcgatag attttagcaa aatgaaggta aactatacaa
     7981 aagatataag aaaaaacaaa ttgcaagaat tgaaaaaaga atttactatg gtttattata
     8041 ctatatcgtc tggacgaaca ttgttattat attgtcattt cgacgaacgc agtgaggaga
     8101 aatcttatta ttatctgtca ttttgacccc gacgtaatcg tgggaagaat ctatcttatt
     8161 agtatcagta aatcaatttt tcaagggaga ggagttttta cgatgattga tacaaagcta
     8221 cttgacattt tagcctgccc tgcatgtaaa ggggatgttg aatatgacgc gcgtaatgaa
     8281 aagatactgt gtacacagtg caaaaagaaa tatcctgtta aagacggtat tcccgtcatg
     8341 cttgtagaag aagccgaaga ataatttaat gtttgatatg aggttatatg tatgtttgat
     8401 tctaaacatc ctgttattaa gatagtgggt tatgttatcg ttggtttttt tgttcttatt
     8461 attataatat cgtttggcat gcctgatttt ttatcccgta tgggttttga tcagaatact
     8521 atcgcaaagg ttaatggcga aacaataggg tatatggatt ttatacgcta tcgtgatacg
     8581 cacatgtttg gtaaaaccga agaccctaaa cagcagcagc gtatgattat agaacgcatg
     8641 atacaggaaa ggctcctggt tcagcttgca aaaaaagagg gcatagtggt aaccgaaaaa
     8701 gaaataaaaa acgttatccg caaccgattt tctgataata ctggaacatt taatgaaaca
     8761 ttttttaaga attttctgga tagattccac atgggtatat cggattatta taaatatgtg
     8821 caacaggaga tatatttagg taaattacag aatctgctgc tggcaggagt cagcgtgtca
     8881 cctctggaac tggtgtctga tttcagaata cagaacacaa aaataaaaat acagtatgcg
     8941 tttgtgtcaa atcaggaact ggcaaagcgg tatgcaaaag aaattacagt taccgatgaa
     9001 gaagttgatg cagaaatgaa gaagaaccca aaagagttga aagacccaaa aactg
//
LOCUS       DAOSZP010000011         8870 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_6709_length_8870_cov_3.382303, whole genome shotgun sequence.
ACCESSION   DAOSZP010000011 DAOSZP010000000
VERSION     DAOSZP010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 8870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8870
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6709_length_8870_cov_3.382303"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /locus_tag="PK348_00555"
     CDS             <1..573
                     /locus_tag="PK348_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018749465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-binding domain-containing protein"
                     /protein_id="HXK64733.1"
                     /translation="YVSKEDTIKWSLVGPVARARGVDIDTRRDHPYAAYDAVQFDVPV
                     VDTCDVWGTVLVRILETFESIKIIRQVLRDMPVDEPLLYEFDEPIKPLVHSISAVEAP
                     RGEVVHYLITGEENRADRWRVRAPTYPNLQGVPLMLLNNQLADAPIIIGSIDPCFSCT
                     DRMALVDLKTGRQSILTNEELERLSRSKKV"
     gene            586..1485
                     /locus_tag="PK348_00560"
     CDS             586..1485
                     /locus_tag="PK348_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020505781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HXK64734.1"
                     /translation="MDILLIVLNIVVFLGLAPLYEGIIRKLTAKVQSRQGPPIYQPYL
                     DLLKLLGKDNLVSDNWAFKLAPMMAFAAVTAVVTIVPIGFKANYLTHYADVITIVYLL
                     TLAGISILLGALSSKNAYAMIGASREMITMIMVEPILAMTFILGAVRMKSLAIDASMF
                     SILSTGYSWSVVLMLVVYLMALQAFVARQPFDISEAEVEILEGPFIEYSGPNYALFKY
                     SMMLKQMFYAVLFVTVFMPFLHTGLFVADLVLQLLAVAVIFVLIALLGSTNPRLRIDQ
                     AVKYYAVLIVISLCTIALSVYGI"
     gene            1510..2055
                     /locus_tag="PK348_00565"
     CDS             1510..2055
                     /locus_tag="PK348_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020505782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HXK64735.1"
                     /translation="MWLTSKIKQLFVVTKPGTVTLPYPFEPRPVPENYRGQPYWDHHK
                     CIGCGGCANHCPARTILVRDLCNELRVMMYDGSRCTYCGRCADLCPEKAITMTPQYEL
                     ATDDKRDINETMELFMLTCQRCGRCFDHEIKNAIDKLAYLGYRYDNLEMRAVIPVSSQ
                     TFDKELYEKTKTYKRPEKIGE"
     gene            2058..2588
                     /locus_tag="PK348_00570"
     CDS             2058..2588
                     /locus_tag="PK348_00570"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018749468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HXK64736.1"
                     /translation="MLGRLAKKAFPKSLWVYHCNSGACNGCDIEILNVLTPYYDVERF
                     GIKLVASPRHADVMLISGAVTRPTFPLVKKAYDAMPSPKLVFGIGSCATGGGCWFDTY
                     NVTGGADKAIPVNYFIPGCPPRPEAIIYGVALALGLVDKKAAPIELKQIEFPIDIYQR
                     TKAWEERNVIYQLLYD"
     gene            2613..3014
                     /locus_tag="PK348_00575"
     CDS             2613..3014
                     /locus_tag="PK348_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020505784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXK64737.1"
                     /translation="MAKKILLVDDDRDLVESLKQALTMNGYEVEVAYSGAEGLKKLLS
                     YHPDLMILDVMMETDTAGFEISYQIRSNREDSKYKDVKNVPIIMLTAINQVTNSRFSL
                     NEEQSFLPGINDFLTKPVRIDELLDKIKKYV"
     gene            3195..5144
                     /locus_tag="PK348_00580"
     CDS             3195..5144
                     /locus_tag="PK348_00580"
                     /inference="COORDINATES: protein motif:HMM:NF026177.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HXK64738.1"
                     /translation="MRKRFATQIRFKLFISILSIVLLSGIASTFVGIRTIEKHVVGQA
                     FDTVLKDLDTADFIYNSRIALKSKMLKHIALLEYIQLAIIHNNRQVLINKCKEVQKEL
                     GIDIVNITDAQGKVIARANNYSFGDDVSDDNHISYVLKNKAPCEGTDIVTHKHLEREG
                     EALAKKVIIDIVPTPKSRKVERTKVVDALAIRATIPIFYNNNFIGIIYGVRILNNYNG
                     IVDQIKELVFKDEKIGGLEVGTSTIFLDDVRVCTNVKKPDGSRAIGTLLSEEVYNKVI
                     LQGKVWWDKAFVVNNWYISAYKPLLNVNNKPIGILYVGILEKKFDEIKTSSTVNFAVI
                     TILIAMVAVGISLYLLNMIIQPVRTLVKASESVINGNYDAHIEIESEDEIGYLCYTFN
                     QMIDAIKERDRKLKEQTQNQILQSEKLASLGRLASGIAHEINNPLTAILTYSSLLLED
                     LKDTDFEEDLRVIIKETFRCRDIVRNILDFARSTKPDIKRTNINNLINEVLLILEKHV
                     NFHNIVINKELDPDVPDMYIDENQFKSVINNLAMNAADAMPQGGTLTVKTLYNLHNDS
                     ITIIVSDTGTGIKEEHLDKIFDPFFTTKEQGKGTGLGLAVTYGIIKRYYGNIDVQSEI
                     GKGTIFTITFPLAKNKEEYAVFQKA"
     gene            5157..5543
                     /locus_tag="PK348_00585"
     CDS             5157..5543
                     /locus_tag="PK348_00585"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXK64739.1"
                     /translation="MKDILARILIVDDEDIVLKSCLRVLQKLDYEIDTAYSGQTALDN
                     LEKKKYDIVVTDLMMPGMDGMQLLEEIKKRYPDVIVIIFTGYATVDTTRQALKAGAFD
                     YIPKPFTPDELRNVIDNAVKSLKNKK"
     gene            complement(5581..7422)
                     /locus_tag="PK348_00590"
     CDS             complement(5581..7422)
                     /locus_tag="PK348_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014957914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HXK64740.1"
                     /translation="MIAVQNLAKRFGSTLLFEDVTFTIGSKEKVGLVGRNGLGKTTLL
                     KIIAGIEQPDEGSVSIPRHYTIGYMSQQLYFTKPVVRDEALSLLPESEQHEYWRAEKI
                     LSGLGFTTSDMLKRVHELSGGFQVRLQLAKTLILNPDMLLLDEPTNYLDITSIRWLAR
                     FLQNWPGELLFITHDRGFMDSIVTHTLGIHRNRIKKIKGNTQQFYEQIALEEEIYEKT
                     RINDERRKKEIEEFISRFRAKARLANLVQSRIKTIEKMQKREKLVPLKNLDFEFRYKP
                     INAKYVLQVDSISFGYDETPLIHNLSFSVAANDRICIIGPNGKGKTTLCKLLAGSLQP
                     QSGTITRALGVETGYFEQTNISTLNPNATIEDEISYSDPDNNKQRARTICGSLLFEGD
                     DALKPVSVLSGGEKSRVLLGKILVKPINLLILDEPSNHLDMEACDALLTAIDSFEGAV
                     ILVTHNELFLRAIAQRLIIFQNNTISIFEGDYERFLEKVGWVSESESTRTGDTDQPES
                     KINKKELRRIRSQLLQQKQSECRPLQQRIEYIENEIMQYDNLLKQCNADIIIASQSQK
                     SSEIQRLSIEINKLEKTLSSLYDELEELMELHEEKNRHYDRLIQEYA"
     gene            complement(7419..7898)
                     /locus_tag="PK348_00595"
     CDS             complement(7419..7898)
                     /locus_tag="PK348_00595"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HXK64741.1"
                     /translation="MVYVYSIIIIIILILLKRMIKIVPRDSICIIEKKLKYHKTLQPG
                     LHIISPFVSSRIVPVEKREGVPVSQEIQFGDSSKQRLTVRTTFLLWDPYTPFFTFEKL
                     DQEMSNKALQELSKLAAGYTKQEFEKNVDVVKQKCLESLNQFGKEYGIEFTSLDITE"
     gene            8171..>8870
                     /locus_tag="PK348_00600"
     CDS             8171..>8870
                     /locus_tag="PK348_00600"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXK64742.1"
                     /translation="MDVEPIRILVLDDDPFIGKMLATMLAGYNVKNTTSFGQFQQVIE
                     EFNPDIAIIDLHLPDAHGLDVCKWIRSQNQYEDVILYILTATNDEQTLIRAYSIGVMD
                     YIIKPFNKFILFSKLRRCALMIEIKRKLEHSLEYQKDIKKRLLQLNDFIKKCIKIDDI
                     DTLVALFLHIKYIIPLDGLFIFNKLTTGKYTVLKLISHESWQSLIPKLHKVIGQNTIE
                     PVSFFFESTTKVYIA"
BASE COUNT         2767 a         1532 c         1808 g         2763 t
ORIGIN      
        1 tatgtttcaa aggaagatac aataaagtgg agtttagttg ggcctgttgc ccgcgcacgg
       61 ggtgtggata ttgatacacg acgcgaccat ccctatgcag catacgatgc agtacaattt
      121 gatgtacccg ttgttgatac ctgcgatgta tggggaactg ttctggtacg tattcttgaa
      181 acatttgaat caataaagat tatcagacag gttttacggg acatgcctgt tgatgaacca
      241 ttgttatatg aatttgatga gcccataaag ccactggttc attcaatatc cgctgttgaa
      301 gcaccacgtg gtgaagtagt tcattacctt attactggtg aagagaacag ggcagatcgt
      361 tggcgagtac gtgcccccac gtatcccaac ttacagggcg taccattaat gttactgaat
      421 aatcagttgg ccgatgcacc aataatcatt ggcagtatag atccatgttt ctcgtgtact
      481 gatagaatgg cactggttga tctaaaaact ggcagacaat caatacttac caatgaagag
      541 cttgaacgtc tgtcacgaag caaaaaagta taaggcaggt ggcatatgga tattctcttg
      601 atagtactta atattgtagt gtttttgggg ttggcaccct tatatgaagg tataatacga
      661 aagctgactg caaaagtaca gtcacgacag ggcccaccta tctatcagcc gtatctggat
      721 ttacttaaac ttttgggtaa ggataattta gtaagcgata actgggcatt caagctggca
      781 ccaatgatgg cttttgcagc agtaacagca gtggttacaa tagttcctat tggatttaaa
      841 gcaaactatt taacacacta tgctgatgtt attactattg tatatttact gacattagct
      901 ggcatctcca ttttgttggg tgcattatca agcaagaatg cgtatgcaat gattggtgca
      961 agccgtgaga tgataaccat gattatggtg gaaccaatac ttgccatgac ctttatactg
     1021 ggtgctgtca ggatgaaaag cctggctatc gatgccagta tgttcagtat tctttccact
     1081 ggctattcat ggtcggtggt actcatgctt gtagtatact taatggcatt gcaggcattt
     1141 gttgcccgtc agccatttga tatttctgaa gctgaagttg aaatactgga aggaccgttt
     1201 atcgaataca gtggtcccaa ctatgcactg tttaaatatt caatgatgtt aaagcagatg
     1261 ttttatgctg tactttttgt aactgtattt atgccattcc tccatacagg gctatttgtg
     1321 gctgaccttg ttttgcagct tttagcagtt gcggttattt ttgtgctgat tgcattactg
     1381 ggatccacca accctaggtt acgtattgac caggcagtaa aatactatgc agtgctcatt
     1441 gtcatatcat tgtgtaccat tgcattaagt gtttatggaa tataaatctt aacgttaagg
     1501 agaatgatcg tgtggcttac aagtaaaata aaacagctat ttgtagtaac taagcccggt
     1561 acggtaacat tgccctatcc ttttgaaccg cgccctgtgc cggaaaatta cagggggcag
     1621 ccatactggg atcatcataa atgtatagga tgtggcgggt gtgccaatca ctgtcctgcc
     1681 cgtaccatac ttgtgcgcga tctttgcaat gagctcaggg tcatgatgta tgatggttca
     1741 cgctgtacct attgcggacg gtgtgcagac ctgtgccctg aaaaagctat aacaatgact
     1801 ccacagtatg agctggcaac cgatgacaaa cgtgacatta atgaaacaat ggaattattt
     1861 atgttaacat gccagcgatg tggaagatgt tttgatcatg aaataaagaa cgcaattgat
     1921 aaactggcat atctgggata ccggtatgat aatttagaga tgagagcagt tattcctgtt
     1981 tcttcgcaga cgtttgacaa agaattgtat gaaaaaacta agacctataa aaggcctgaa
     2041 aagataggag aatagccatg ttgggtagat tagcaaagaa ggcatttcca aagtcattgt
     2101 gggtgtatca ctgcaattct ggtgcgtgca atgggtgtga tatagagata ttaaatgtat
     2161 taactcctta ctatgatgtg gaacggtttg gcatcaaact tgttgcatca cccaggcatg
     2221 ctgatgtgat gcttatttca ggtgcagtta ccaggccaac attcccccta gtaaaaaaag
     2281 cgtatgatgc aatgccatca ccaaaacttg tatttgggat tggatcatgt gcaacaggag
     2341 gcggttgctg gtttgatact tataatgtaa caggtggtgc tgataaggca ataccggtta
     2401 actattttat tcctgggtgt cctccaaggc ctgaagcaat tatttatggt gttgcattgg
     2461 cgttaggtct ggtggataag aaggctgctc ctattgaact gaagcagata gaattcccaa
     2521 tagatatcta tcagcgaacg aaggcatggg aagaaagaaa cgtcatttat cagctgctct
     2581 atgattaaaa tttggtagat ggagtataca caatggcaaa aaaaatatta ttagttgatg
     2641 acgatagaga cctggttgaa agcctgaagc aggctctgac aatgaatggc tatgaggtgg
     2701 aagttgccta tagtggtgct gaaggcttga aaaagttact atcgtatcat cctgatctta
     2761 tgattttaga cgtcatgatg gaaactgata ctgcaggttt tgagatatca tatcagatac
     2821 gaagcaacag ggaagattca aaatataagg atgtaaaaaa tgtaccaatc attatgctga
     2881 cagcaataaa tcaggtgact aattcacggt tttcactgaa tgaagaacaa tcatttctac
     2941 ctgggataaa tgattttttg acaaaaccgg tacgcataga tgaattgctg gataaaatca
     3001 aaaaatatgt atgagtttac cgtgatgtgc atatagtatc acaacaacct gtttacaatc
     3061 gcaaagccgg aatgtcagga tcagatgttc cggctatttt ctatttttgc ttgtgcatga
     3121 ataaaaaggt tgcaaaatat atgcaataag atttattata tgtaatacaa ttaaatgaat
     3181 gattacataa caatatgaga aagcgatttg ccacacagat acggtttaaa ttatttatta
     3241 gcatactttc cattgtgctt ctatcaggta ttgcttcaac ctttgttggc atacggacaa
     3301 ttgaaaaaca tgttgttggg caggcttttg atactgtatt gaaagattta gacacagctg
     3361 attttattta caatagcagg attgctctta aaagtaaaat gctcaagcat attgcgctgt
     3421 tagagtatat tcaattggca attatacata ataaccgcca ggtgttaatt aataaatgca
     3481 aagaagtgca gaaggaattg ggaattgata ttgtcaatat tacagatgct caggggaaag
     3541 tgattgcccg ggcaaataat tattcatttg gtgatgatgt ttccgatgat aaccatattt
     3601 cgtatgtatt aaaaaataaa gcaccgtgtg aaggaacaga tattgtcacc cataaacatc
     3661 ttgagcggga aggagaagca ttagctaaaa aagtaattat tgatattgta ccaactccta
     3721 agtcaagaaa agttgaacgg acaaaggttg ttgatgctct agcaatacgg gccaccatcc
     3781 ctatatttta taataataat tttattggta ttatttatgg cgtgaggatt ttaaataatt
     3841 ataacgggat agtagaccag ataaaggaac tggtattcaa agatgaaaag attggaggac
     3901 tggaggttgg tacttcaaca atattcctgg atgatgtcag agtatgcacc aatgtaaaaa
     3961 aacctgatgg ttcacgagca attggcacac tgctttccga ggaagtatac aataaggtca
     4021 tattgcaggg aaaggtctgg tgggacaagg catttgttgt taataactgg tatatatctg
     4081 cgtataagcc actgttaaat gtaaacaata aacccattgg tattctttat gttgggatac
     4141 ttgaaaaaaa atttgatgag attaagacaa gttcaactgt caattttgca gtcattacaa
     4201 ttttaattgc aatggttgcg gttgggattt ctctgtatct tttaaacatg attatccagc
     4261 cggtgcgaac actggttaaa gcatctgagt cagttattaa tggaaactat gatgcacaca
     4321 ttgagataga aagtgaagat gagatagggt atttgtgtta tacatttaac cagatgattg
     4381 atgcaataaa agaaagagat agaaagctaa aagaacaaac tcaaaaccag atattgcaat
     4441 ctgaaaaatt agcttcactt ggcaggttgg catcgggaat tgctcatgaa ataaataacc
     4501 cccttacggc cattcttacc tacagttccc ttttgctgga agatttgaag gatactgatt
     4561 ttgaggaaga cttaagagtt attattaaag aaacgtttcg atgcagggat attgtacgca
     4621 acattttaga ttttgcccgt tcaacaaaac ctgatataaa acggactaat attaataatc
     4681 ttataaatga agttcttctg attcttgaga agcatgttaa ctttcacaac attgttataa
     4741 ataaagaact ggatcctgat gtgcctgata tgtatataga tgaaaatcag tttaaatcgg
     4801 ttattaataa tctggctatg aacgcagcgg atgcaatgcc acagggaggc acgctgacag
     4861 tgaaaacact gtataattta cataatgata gtatcaccat aattgtttct gatactggaa
     4921 caggaatcaa ggaggagcat ttagataaaa tttttgatcc gttctttact acaaaggaac
     4981 agggaaaggg tactggactt ggccttgctg taacctatgg tattatcaag cgatactatg
     5041 gtaatattga tgtgcaaagt gagataggga agggaacaat ttttaccatt acgttcccat
     5101 tggccaaaaa taaagaagaa tatgcagtat ttcaaaaagc atgaaaggtg tacgttatga
     5161 aagatatact tgcaagaatt ctgattgtag atgatgagga tatagtactc aaaagctgtc
     5221 tcagagtgct gcaaaaactg gattatgaaa ttgacaccgc atattcaggg caaactgcgc
     5281 tggataatct tgaaaaaaag aaatatgata ttgttgtcac cgatctcatg atgccgggta
     5341 tggatggaat gcagcttctg gaagaaataa aaaaacggta tcctgatgta attgtcatta
     5401 tatttacagg ttatgcaaca gttgatacaa cacgacaggc attgaaggca ggggcatttg
     5461 attatattcc caagccgttc actcctgatg agttgcgcaa tgttatagac aatgcagtta
     5521 aaagcttaaa aaataaaaaa taatagcgag tgaaatcagt caaaagttat tattatttcg
     5581 ttatgcatat tcctgaatta atctgtcata gtggcggttt ttttcttcat gcaattccat
     5641 aagctcttca agttcatcat acaagctgct taatgttttt tcaagtttat taatttcaat
     5701 ggataaacgt tgaatttcac tgctcttctg tgattgtgat gcaatgataa tatcagcatt
     5761 acattgtttt aaaaggttgt catactgcat aatttcattt tcaatgtact caatgcgctg
     5821 ctgcaatggt ctgcattcac tttgcttttg ctgcaacaat tgtgagcgta tccttcgtaa
     5881 ttctttttta ttgattttgc tttcaggctg atcagtatca cctgttcttg tactttctga
     5941 ttcagaaacc cagcccacct tttccagaaa gcgttcataa tcgccttcaa aaatgcttat
     6001 agtattattt tgaaaaatga ttaatcgctg tgcgatagct ctaaggaata attcattatg
     6061 cgtaacaagt atcactgcac cttcaaagct gtcaatagcc gtaagcaatg catcgcaggc
     6121 ttccatatct aagtgattcg atggctcatc cagaataaga agatttatag gcttaaccag
     6181 aatttttccc aaaagaaccc ggctcttttc accgccagat agaaccgata caggtttcaa
     6241 tgcgtcatca ccttcaaaaa gaagtgagcc gcatatagta cgtgcacgct gtttgttgtt
     6301 atccgggtcg ctgtaggaaa tctcatcttc aatagtagca ttggggttta atgttgaaat
     6361 attagtttgt tcaaagtatc ccgtttcaac gcctaatgcc ctggtgatag ttcctgattg
     6421 tggttgcagg ctgccagcca aaagtttaca cagtgtggtc ttgcctttac catttggacc
     6481 tattatacag attctatcat tggcagctac tgagaatgaa agattgtgta ttaaaggtgt
     6541 ttcatcgtag ccaaatgaaa tagaatcaac ctgtaaaaca tattttgcat ttatgggctt
     6601 atacctgaat tcaaaatcaa gattttttaa tggtacaagc ttttctcttt tctgcatctt
     6661 ctctatggtt ttaattcggg actgtaccag gttggcaagc cgtgcttttg cccggaatcg
     6721 tgaaataaat tcttctattt ctttttttct gcgctcgtcg ttgatgcggg ttttttcata
     6781 tatttcttct tcaagcgcaa tttgctcata gaattgctgg gtattgccct ttattttttt
     6841 tattcggttg cggtgaatcc caagagtgtg ggtgacaata gaatccatga atcctctgtc
     6901 atgcgtaata aacagaagtt cacctggcca gttctgtaaa aagcgggcta accagcgaat
     6961 agaggtaata tcaagataat tggttggctc atccagtagc agcatatcag ggttcagtat
     7021 cagtgttttt gcaagctgca ggcgcacctg aaatccccct gaaagctcat gcaccctttt
     7081 tagcatgtca ctggtggtaa atccgagccc tgataaaatc ttttcagcac gccagtattc
     7141 atgttgctca gattcaggca aaagcgataa tgcttcatcg cggactaccg gttttgtaaa
     7201 ataaagctgc tgggacatgt acccaatagt ataatggcga gggatagaca cactgccttc
     7261 atcaggctgt tcaatgccgg caatgatttt cagcaacgtt gtctttccca gcccgttgcg
     7321 gccaacaagc cccacctttt ctttactgcc tattgtaaag gtaacatctt caaaaagaag
     7381 tgtgctgcca aatcgtttag ctaaattttg aactgcaatc attctgtaat atctaagcta
     7441 gtaaattcaa taccgtactc tttcccaaac tgatttaatg attcaagaca tttttgtttc
     7501 acgacatcaa catttttttc aaattcctgt tttgtataac cagcagcaag ttttgacagt
     7561 tcctgcaatg ctttattgct catttcctgg tctaatttct caaaggtaaa aaaaggtgta
     7621 taggggtccc ataacaggaa tgttgtcctg actgttaatc tttgtttgga actatcgcca
     7681 aattgtattt cctgtgatac cggcacgccc tccctttttt caacgggaac aatgcgcgac
     7741 gaaacaaatg ggctaataat gtgtaatccc ggctgtaacg ttttatgata tttaagcttt
     7801 ttttcaatta tgcagataga atcacgaggg acaatcttta tcattctctt taaaagaatt
     7861 aaaataataa tgataataat actgtacaca tacaccatag tgttacctcc acaaaaataa
     7921 tacatactgt atatattgtg ttacaggttt ataatattac cagaacatta taaaagtata
     7981 tgtacaccag agtataaatt atttcaacaa aagttttaat tattttagcg atagttcata
     8041 ctatgtaaaa tagtatttga ttatacccgc aagaccaata tgagcatgca aatatttact
     8101 gacctgtaaa aatacacttg aaaaaagtgt ttcagggata tacaatgttt tttgttacag
     8161 gatcgctact atggatgttg aaccaatacg aattcttgtt ctggatgatg accccttcat
     8221 tggcaagatg cttgccacaa tgttagctgg ctataatgtg aaaaatacaa catcgtttgg
     8281 ccagttccag caggtcatag aggaatttaa cccagatatt gcaattatag atttacattt
     8341 gccagacgca catgggcttg atgtgtgtaa atggatcaga agccagaatc agtatgagga
     8401 tgttattttg tatatattga ccgctacaaa cgatgaacaa acactcatca gagcatacag
     8461 cattggcgtt atggattata tcataaagcc ttttaacaag tttattctat tttcaaaatt
     8521 acgccgatgt gctctgatga ttgagatcaa acgtaagctt gagcattctt tggaatatca
     8581 aaaagatata aaaaaacgat tattacagct gaatgatttt attaaaaagt gcattaaaat
     8641 tgatgatata gatactcttg tagcattatt cctccatatt aaatatatta ttccgcttga
     8701 tggattattt atatttaaca aacttacaac agggaaatat acggtattga aattaatttc
     8761 gcatgaatcc tggcaatcgc tcattcctaa gcttcacaaa gttattggtc aaaatactat
     8821 cgaaccggtc tcttttttct ttgaatccac tactaaagta tatattgcca
//
LOCUS       DAOSZP010000012         8737 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_6850_length_8737_cov_3.417531, whole genome shotgun sequence.
ACCESSION   DAOSZP010000012 DAOSZP010000000
VERSION     DAOSZP010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 8737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8737
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6850_length_8737_cov_3.417531"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="PK348_00605"
     CDS             complement(<1..351)
                     /locus_tag="PK348_00605"
                     /inference="COORDINATES: protein motif:HMM:NF012725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXK64743.1"
                     /translation="MYKKIITVLFCICVTALYAQDPEELTREGVELGRKQKFDESLQK
                     LDESIKLYNVRSAKTLHNMGYVYELKGEIDKAIPAYEEAVRRNPVQIPSLERLGYLYY
                     KTGLFDKAVATGEHV"
     gene            535..2796
                     /locus_tag="PK348_00610"
     CDS             535..2796
                     /locus_tag="PK348_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015680943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HXK64744.1"
                     /translation="MKKTIMVIGGGLLQVPVIQTAQKMGLQVIVTDYNPNALGMKYAD
                     IPIVMSTRDIEGSVRVAKKQHEITPISGVLTVGTDASATVAAVANALNLPGIKFEDAI
                     AATNKIKMRTRFFEHGVPSPHFFPVWSLSDAKKACKVLGFPVVIKPSDNMGARGVVRI
                     DNKNQIADAFAFAKSASPSGELIIEEFMEGHELSIDAVVFNGEVTFTGIADRIIEYPP
                     HFVETGHTMPSQLPKEVQDSACEVMKAGIKALGIHLGCAKGDIKITKDGPKIGELAAR
                     LSGGFMSAYTYPLSTGVNLMRAAIEVALGQEPGNLEPLYNRVSIERAIITKPGIVKKI
                     CGVEEALKIPGIAEIFITVKPGDKVKKPTSNVEKAGHIIAVADTLKEAEEAVRKCKEM
                     IHIEICDESELSMDTINISARERLRKVCYVCKQCNGKDCASGVPGMGGAGSGASFKRN
                     SEALLNYKINTRVIHDVTNPDTSTSLFSINLSFPVMAAPITGAITNLGGAIDELDYNI
                     AVVKGCMDAGTIAFLGDGADPEKYKIAMEALSQFGGMGIPIFKPRANNEEIIKRIKAA
                     ETAGAIAVGMDIDAIVFKTMELKHQAVKPKSLTELKSIISSTHLPFILKGIMNPRDAA
                     MAVEAGAHAIVVSNHGGRVLDEMVGTMDVLEDIVREVHGHIHILIDGGFRTGVDILKA
                     LALGAEYVLIGRPVAIAAVGMGSEAVAYYLKSLQAELKKAMILTGCATIHDITGDIVR
                     KIHHNSYQPLEVR"
     gene            3026..3475
                     /locus_tag="PK348_00615"
     CDS             3026..3475
                     /locus_tag="PK348_00615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK64745.1"
                     /translation="MIKLSRWYIAIAIVTVTIATHALQFTDIHGIWQLLYRGNYGYEF
                     RFYKNYQAVCIIYMQTNAVVFKGVYTFDNPQTIRININQMKNADSCCNVNTTTGFAQV
                     KSSYFLFNATLITKNNKKMLELKPLSIVIDGINSEGYFEPVILLQRM"
     gene            3481..4218
                     /locus_tag="PK348_00620"
     CDS             3481..4218
                     /locus_tag="PK348_00620"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HXK64746.1"
                     /translation="MSYRTITIPYKDPIDAVVYPCSQSVGTIVYSHGLFSSKDAYKIH
                     QIAPALHDSGYTVISFSYGYTRHYTTGNALTIALPQCADELNAVLEYAANTYNPSLHI
                     IGSSMGGAIALYYAATVPYIFSLKSLSIIATPVHINHLVRAIAPQGISGNYVTVGEYT
                     IDAQSLQQMNTINLIKLIPRVDIPVCIIHGQNDDVVPVAHAHDIQKNLCVPHTMHIIP
                     DGDHTLTAQRHVELILQYIVSWLKLHP"
     gene            4335..5198
                     /locus_tag="PK348_00625"
     CDS             4335..5198
                     /locus_tag="PK348_00625"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013447291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HXK64747.1"
                     /translation="MLTNLNQCPNPIEYQNQNINEDIFNYLTEKLKEKTILHYKKAIL
                     LQGDVLNKDYFDNDIFDLIVTSPPYNVDIKYNTHKDNISYEEYLQFSEAWMSNCYKWS
                     KKQARLCLNIPLDKNKGGQRSVGADLTILAQKVGWKYHSTIIWNEGNISRRTAWGSWL
                     SAAAPYVIAPVELIVILYKEEWKKTQGSKISDISKDEFMQWTNGLWTFNGESKKRIGH
                     PAPFPLELPYRCIKLFSFVHDIVFDPFAGSGTTLIAANNINRYSIGLEIDSSYCELSK
                     QRILQENGMLF"
     gene            5214..5798
                     /locus_tag="PK348_00630"
     CDS             5214..5798
                     /locus_tag="PK348_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004275842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HXK64748.1"
                     /translation="MQKQKTITDLIIEFFKNHPKEDMPHGPVVDWVEEQYTKLYNRKP
                     RDIWRSIRRLHQEGFLIKVKKGIYRYDPDIANNKHLEEFSQELKNQILERDNYKCVIC
                     GRTAKEGYELHVDHILPKDKGGKATLDNGQTLCSICNFRKKNYNQTESGKRMFIRLLK
                     IAKEVGDIKTQKFCEEILNTYEKFNINGHIKWDK"
     gene            5985..7442
                     /locus_tag="PK348_00635"
     CDS             5985..7442
                     /locus_tag="PK348_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006570020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase family
                     protein"
                     /protein_id="HXK64749.1"
                     /translation="MPLKTPAEYIESIKALKRTVYLFGQKIDNTVDNPIIKPSLNAVA
                     MTYALAQKEEYRDIMTATSHITGNVINRFTHIHQSIDDLVKKSKMGRLLGAKTGCCFQ
                     RCVGMDALNALSMTTYAIDQKYGTQYYQRFLKYLQYVQDNDLVCDGAMTDPKGDRSLP
                     PHKQEDPDLYLHVVKETKDGIIVRGAKAHQTGALNSHEVIVMPTIAMRPEDRDWAVSF
                     AVPADAEGIIYIYGRQSCDTRKLEKYSIDQGNALFGGHEALIVFDDVFVPWDRVFMYK
                     EYDFAGHLVERFASYHRQSYACKVGVGDVLIGATQTIAEYNGIDKASHVKDKIIEMIH
                     LNETLYCGCIACATEGKREEPGTYMVNTLLANVHKQNITRFPYEIARLAQDIAGGALV
                     TLPSADDLNHPEAGKWIKKYFKAKSDIPTEHRIRILRLIENITMGTAAVGYLTESMHG
                     AGSPQAQRIMIARESNVKEKQKTAQRLAQVISSDE"
     gene            7632..8078
                     /gene="tnpA"
                     /locus_tag="PK348_00640"
     CDS             7632..8078
                     /gene="tnpA"
                     /locus_tag="PK348_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010609444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HXK64750.1"
                     /translation="MSTYTQILYQLVFSTKYQEMILLKENRPKLYQYITGILKNKNCH
                     LYQINGIDNHVHILFHLHPSQSLSDLVKDIKLASSKFIKENHLFPNFIGWQNGYGAFT
                     YSIEAKDNLIAYIKNQESHHKIKSFRDEFIELLVEHGIIFDEKFVL"
     gene            complement(8248..>8737)
                     /locus_tag="PK348_00645"
     CDS             complement(8248..>8737)
                     /locus_tag="PK348_00645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK64751.1"
                     /translation="KALSGVKFDYSVQRGFQFGAGMFSISATNDVIAINDDVRMGINF
                     GYIKQATGTEWAIRRVMLSEYQKDNYFVLVNWVAPDTRLPKDYINYWESIVNAEHPYT
                     MKPFTSDNSTKIAAVVKKIQEKPEKVKKLYTLFLGKAGKVEEDRMIQDFKKLMNGIVI
                     NE"
BASE COUNT         2934 a         1656 c         1625 g         2522 t
ORIGIN      
        1 aacatgctca cctgttgcaa cagccttgtc aaataatcct gtcttatagt ataaataacc
       61 aagcctctcc agtgatggta tttgtacagg attgcgccgc acggcctctt catatgcagg
      121 gatagcttta tcaatttcac ctttgagttc ataaacatat cccatattat gcaatgtctt
      181 ggccgagcgt acattatata acttaattga ttcgtccagt ttctgcagtg attcatcaaa
      241 tttttgcttc ctgcccagtt caaccccttc tctggttaat tcttcagggt cctgagcata
      301 cagagccgtc acacaaatgc aaaataatac agtaattatt tttttgtaca taacccttcc
      361 cttactttac caatttataa caattaacag tactatatac tattgagaaa tgccacagaa
      421 atgcaactaa aaattacatt taagttttat tctaaaaaag ccaataatta taacagtata
      481 agtattgctc tattcgcaat gtaccgctaa tgtgacataa tatagggggg catcatgaaa
      541 aagaccatta tggtaattgg tggaggcctt ttacaggtac cagttattca gacagctcaa
      601 aaaatggggc tacaggtgat agttactgat tataacccca atgctctggg aatgaagtat
      661 gctgatatcc ccattgtcat gagtacccgt gatattgaag gttcagtccg cgttgcaaaa
      721 aaacagcatg agattacccc tatatctggc gttttgactg ttggaactga tgcttcagcc
      781 actgtcgctg ctgtagcaaa tgcacttaac ctccccggta taaaatttga ggatgccatt
      841 gctgcaacca ataaaattaa aatgcgcacg aggttttttg agcacggtgt tccttcacct
      901 cattttttcc ctgtatggtc tctttctgat gcaaagaagg catgcaaggt tctgggattt
      961 cctgtcgtta taaaaccatc tgataatatg ggcgcacgcg gtgtggtacg tattgacaat
     1021 aaaaaccaga tagctgatgc atttgcattt gccaaaagcg cctcacccag tggcgaattg
     1081 attatagaag aatttatgga aggccatgaa ttatccatag atgctgttgt atttaacggt
     1141 gaagtaacct tcaccggcat tgccgaccgc atcatagaat atcctccaca ctttgttgaa
     1201 actgggcaca ccatgccatc acagttgcca aaagaagtgc aggacagtgc ctgtgaagtt
     1261 atgaaagccg ggattaaagc gttaggaatt catcttggtt gtgccaaagg tgatattaaa
     1321 ataacaaagg atggcccaaa aattggggaa ctggctgcca ggctttccgg tggttttatg
     1381 tctgcgtata cctacccgct ttcaaccggc gttaatttaa tgcgtgctgc cattgaggta
     1441 gcattgggac aggaaccagg caaccttgaa ccactgtata acagggtatc gattgaacgc
     1501 gcaatcatca caaagcctgg tatagtaaaa aagatatgtg gtgtagaaga agcattgaag
     1561 atccccggca ttgcagaaat attcattacc gtaaaacctg gtgataaagt aaaaaaacca
     1621 acatccaatg ttgaaaaagc aggacatatc attgctgttg ctgatacact gaaagaagca
     1681 gaagaagctg tacgaaagtg caaagaaatg atacacatag aaatatgtga tgaatcagaa
     1741 ctttccatgg ataccatcaa catttctgca cgggaaaggt tgcgcaaagt atgctacgta
     1801 tgcaagcagt gcaatggcaa ggattgcgcc agcggcgttc ccggtatggg cggtgcaggc
     1861 agtggagctt cattcaagcg aaacagtgaa gcactcctta attataaaat caatactcgt
     1921 gtcattcatg atgtaaccaa tcccgataca tctacttcac tttttagcat caacctgtcc
     1981 ttccccgtta tggcagcacc cattaccgga gctattacca atttaggtgg ggcgatagat
     2041 gaattagatt acaatatagc tgttgtaaaa ggatgcatgg atgcaggcac cattgcattc
     2101 ttaggtgacg gcgcagatcc agaaaaatat aaaatagcta tggaagcgtt atctcaattt
     2161 ggcggtatgg gcatacccat attcaagcca cgagcaaata acgaagaaat tattaaacgc
     2221 attaaagctg cagaaacagc aggcgctatt gctgttggca tggatattga tgccattgta
     2281 ttcaagacca tggaattaaa acatcaggca gtaaagccaa aaagcttaac cgagcttaaa
     2341 agcataatat catccacaca tctgccattt atactcaaag gcattatgaa tccacgcgat
     2401 gctgctatgg ctgttgaggc tggcgcacat gcaattgttg tatccaacca tggcggcaga
     2461 gtacttgatg aaatggtggg caccatggat gtacttgaag acattgtgcg cgaggtacat
     2521 ggacacattc atatattaat tgatgggggt tttagaacag gggttgacat tttaaaagca
     2581 ttagcattag gtgctgaata tgtgcttatt ggcaggcctg ttgccattgc tgctgtggga
     2641 atgggttctg aggctgttgc ctattacctt aaatcattac aggcagagct taaaaaagcc
     2701 atgatactga caggctgcgc aacaatacat gatatcacag gtgatatcgt tcgcaaaata
     2761 catcataatt cataccagcc actagaggta cgatagtaca ttgtatggga aatgctctat
     2821 tattactact gctcaatgca gcggtgccaa tacatttcat tatttttaat tgaaaaatat
     2881 tttttaaaaa ttataacact attccaaatt ttcagaaagt ggactattat gtattcccgc
     2941 atattaaaga cattgcttta aaaaattcct tgattattag tcagattatt cttactgtgt
     3001 taaacattac aacgaaggga agtaaatgat caaattatcc agatggtata ttgcaatagc
     3061 tattgtaaca gtaactatcg ccacgcatgc acttcagttt actgacattc atggtatatg
     3121 gcaattactc taccgtggca actatggata tgaattcaga ttctataaaa attaccaggc
     3181 tgtgtgcatt atctacatgc agaccaatgc cgttgtcttc aaaggtgtat atacctttga
     3241 caatccacaa accattcgca ttaacattaa ccagatgaaa aatgccgata gttgctgtaa
     3301 tgtcaatacc actactggct ttgctcaggt aaaatcttca tacttccttt ttaatgctac
     3361 attgataacc aaaaacaaca aaaaaatgct tgaattaaag ccgctgtcca ttgtcattga
     3421 tggaattaat tctgaaggat attttgaacc agtcattcta ttgcagcgca tgtaaccacc
     3481 atgagctatc gaacaattac tattccgtat aaagacccca tagatgcggt tgtgtatccc
     3541 tgttcccaaa gtgtgggtac cattgtgtac agccatgggc tcttttcttc caaagatgcc
     3601 tacaagatac atcagatagc accagccctt catgatagcg gctatacggt gataagtttc
     3661 agctacggat acactcgtca ctatacaacc ggaaacgctc ttacgattgc gctgccacag
     3721 tgtgcagatg aactcaatgc agtgctggaa tacgccgcca atacttacaa ccctagcctt
     3781 catattattg gttcaagtat gggtggtgct atagcgctgt actatgcagc aaccgtgcca
     3841 tacatttttt ctttaaaaag cttaagcatt atagcaacac ctgtgcatat aaatcattta
     3901 gtcagggcga tagctccgca aggcatctca ggtaattatg taaccgttgg cgaatacaca
     3961 attgatgcac aatcgttgca acagatgaat accattaacc tgataaaact tatcccacgc
     4021 gttgacatcc ccgtgtgtat tattcacggg cagaatgacg atgtggtgcc agtagctcat
     4081 gcccatgata ttcagaagaa tctatgcgta ccccatacca tgcacatcat ccccgacggc
     4141 gaccatacat taacagcaca aaggcatgtt gaattaatct tgcaatacat agtgtcatgg
     4201 cttaaactgc atccgtagta ccttttgcta ttaatacaaa ttgaataaac cttgacataa
     4261 tattgttaac atattatatt tgtattgtta gaagttaaaa tatatggagc atgtatatat
     4321 aaaaggttaa aataatgctt accaatctaa atcaatgtcc aaatcctata gaatatcaaa
     4381 accaaaatat aaatgaggat atatttaatt atttaacgga aaagttaaaa gaaaaaacta
     4441 ttcttcatta taaaaaagca attcttttac aaggagatgt tttaaataaa gactattttg
     4501 ataatgacat ttttgattta atagttacat cgcctcctta taatgttgat atcaaatata
     4561 atacacataa agacaacatt tcttatgaag aatatcttca attttcagaa gcatggatga
     4621 gcaattgtta taaatggagc aagaaacaag cacgcttatg tttaaacatt ccactggata
     4681 aaaataaagg cgggcagcgc agcgtaggtg cagatcttac aatacttgcc caaaaagtag
     4741 ggtggaaata ccactcaaca attatctgga atgaaggtaa tatctcacgt agaacggctt
     4801 ggggatcatg gttatcagca gcggccccat atgttattgc acctgttgaa cttattgtaa
     4861 tactttataa agaagaatgg aaaaaaactc aaggttcaaa aatttcagat atatcaaaag
     4921 atgaatttat gcaatggaca aacgggttat ggacattcaa tggagaaagt aaaaaaagaa
     4981 ttggtcatcc agcaccattc ccattagaat taccatatag atgtataaaa ttatttagtt
     5041 ttgttcacga tatagttttt gatccatttg ctggtagtgg aactaccctc atagcagcaa
     5101 ataatattaa tagatattca attggattag aaattgattc ctcatattgt gagctctcaa
     5161 aacaaaggat attacaagaa aatggaatgc tattttaatt atgtaaatat attatgcaaa
     5221 aacaaaaaac tattactgat ttaatcattg aattttttaa aaaccatcct aaagaagata
     5281 tgccccatgg accagtagtt gattgggttg aagaacaata taccaaatta tataatagaa
     5341 aaccacgaga catatggcgt tcaatacgtc gtttgcacca agaaggtttt ttaataaaag
     5401 tcaaaaaagg tatctataga tatgatcccg atatagcaaa caataaacat cttgaagaat
     5461 tttctcaaga attaaaaaat caaattttag aacgtgataa ctataaatgt gtaatatgtg
     5521 gacgtactgc taaagaagga tatgaattac atgtagatca cattttacca aaagataagg
     5581 gaggtaaagc tacattagac aacggacaaa cactttgttc aatttgtaat ttcagaaaga
     5641 aaaattataa tcaaacagaa agcggtaaaa gaatgttcat tcgtttgtta aaaattgcaa
     5701 aagaagttgg agacattaaa acacaaaaat tttgcgaaga aattttaaac acttatgaaa
     5761 aattcaacat aaatggacat ataaaatggg ataaataaaa agtttctaat atccaataga
     5821 tatattatta tattataaaa aaatttttta gaaaactctt tgttggcagt aaattctatt
     5881 aagcaattca attattataa tacatttatt attgacaaac ttaataaagt aatgtaaatt
     5941 tgtatttatg aatgtacatt aaccttttag aaaggagatt acctatgcca cttaaaaccc
     6001 ctgccgaata tattgaaagt attaaagcat taaagcgaac cgtgtatctt tttggtcaga
     6061 aaattgataa taccgttgat aatcccatca tcaaaccatc acttaatgcg gttgctatga
     6121 cgtacgccct tgctcaaaaa gaagaataca gagatatcat gacagctaca tcacatataa
     6181 ccggcaatgt cataaaccgt tttacccata ttcatcagag cattgatgat ctggtgaaaa
     6241 aaagcaagat ggggcgtttg cttggagcaa agacgggatg ctgcttccag cgctgtgtag
     6301 gtatggatgc gctcaatgca ctatcaatga caacatacgc aattgatcag aaatacggca
     6361 cacaatacta tcagcgcttt ttaaaatatt tacagtatgt acaggataat gacctggtat
     6421 gtgatggtgc aatgaccgat cccaaaggag accgttcact gccacctcat aaacaggaag
     6481 atcctgatct gtacctgcat gtggtaaaag aaactaaaga tggcattatt gtgcgcggtg
     6541 caaaggctca tcaaaccggt gctctgaatt cacatgaagt tattgttatg ccaactatcg
     6601 ccatgcgccc tgaagacagg gactgggctg tgtcctttgc agtaccggct gatgctgaag
     6661 ggattattta catctatgga cggcagtcct gtgacacccg taagttagaa aaatatagca
     6721 tagatcaggg aaatgccctg tttggtggtc atgaagcgct gatagtattt gatgatgtgt
     6781 ttgtaccctg ggatagagtc tttatgtaca aagaatatga ctttgcaggt cacttagtag
     6841 aacgctttgc ctcataccac cgccagagtt atgcctgcaa ggttggcgtg ggagatgtgt
     6901 taattggtgc aacacagaca atagccgagt acaacggcat tgacaaagca tcgcatgtga
     6961 aagataagat cattgagatg atacacctga atgaaacgct gtattgcgga tgtattgcct
     7021 gtgcaacaga gggcaagcgt gaagagcccg gcacctatat ggttaacacc ctgcttgcca
     7081 atgtgcataa gcagaatatc acgcgctttc catatgagat agcacggtta gctcaggaca
     7141 tagccggtgg tgcactggtt accctaccat cagctgatga cctcaatcat ccagaagcag
     7201 gcaaatggat taaaaaatat ttcaaagcaa aaagcgatat ccctaccgaa caccgcatac
     7261 gtatcctgcg cttgatagaa aacattacca tgggaacagc tgcggtagga tatttaaccg
     7321 aatcaatgca cggagcaggt tcgccacagg cacagcgcat tatgatagca cgcgaatcca
     7381 acgtgaagga aaagcaaaag acagcgcagc gcctggcaca ggtaatttct tctgatgaat
     7441 aatatttttt ttcaatcgcg aagcgattaa atatgaatag ccatggatgt aatccgtggc
     7501 tattcaccat caatacacaa aaccccaaag gggtttaata tgattgacag aatttttaat
     7561 gtactataca tcttcaatat attaaatata aaccataata tctcaaagca ttacaaccaa
     7621 taaaatttga tatgagtaca tacacacaaa tattatatca attagtattc agcacaaaat
     7681 atcaggaaat gatattatta aaagaaaata ggcctaaatt atatcaatat attactggta
     7741 tattaaaaaa taaaaattgc cacctatacc aaataaatgg catagacaat catgttcata
     7801 tattatttca tcttcacccg tcgcaatcat tgtcagattt agtaaaagat ataaaattgg
     7861 caagttcaaa atttattaaa gaaaatcatc tattcccaaa ctttatagga tggcagaatg
     7921 gttatggagc atttacttac tcaattgaag cgaaagataa tcttatcgct tatataaaaa
     7981 atcaagaatc acatcataaa ataaaaagtt tcagagacga gtttattgaa cttttagtag
     8041 aacacggaat tatttttgac gaaaaatttg tattataaaa tttcaaccct tccagggttg
     8101 aaaaagtttt tgatttattt tctccgcatt acatacggag ctattactat ttaatccctt
     8161 cgggattata gtaaaactat ctttgtattt acacttaaat cttaacatat agccgcgtga
     8221 cacatacgcg gctataattg tattcaatta ttcattaata acaatcccgt tcatgagttt
     8281 tttgaaatcc tgaatcattc tgtcttcttc cacttttccg gctttcccta aaaacagcgt
     8341 gtacagcttc ttaacttttt caggtttttc ctggatcttc ttaaccacgg cagctatctt
     8401 tgtggagtta tcgcttgtaa atggcttcat ggtatacgga tgctcagcat tgacaatgct
     8461 ttcccagtaa ttaatataat cctttggcaa cctggtatcg ggtgcaaccc agtttaccag
     8521 tacaaagtaa ttatctttct gatattcact caacatgaca cggcgtattg cccattctgt
     8581 gcctgtggcc tgttttatat atccaaaatt gatacccata cgcacatcat cattgattgc
     8641 aatgacatca tttgttgccg aaatagaaaa catacctgca ccaaactgga atcctctttg
     8701 cactgaataa tcaaacttga ccccgcttaa tgctttt
//
LOCUS       DAOSZP010000013         8453 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_7162_length_8453_cov_3.505835, whole genome shotgun sequence.
ACCESSION   DAOSZP010000013 DAOSZP010000000
VERSION     DAOSZP010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 8453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8453
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7162_length_8453_cov_3.505835"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..298)
                     /locus_tag="PK348_00650"
     CDS             complement(<1..298)
                     /locus_tag="PK348_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018247992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP cone domain-containing protein"
                     /protein_id="HXK64752.1"
                     /translation="MTDSKDSPQFIRKRDGRIVPFNIEKITEAIFKAARAVGGTDRNT
                     AAQIAESVVDILSLLYKGEHIPTVENVQDLVEKMLIEKGHAKVAKAYILYREQHR"
     gene            575..1096
                     /locus_tag="PK348_00655"
     CDS             575..1096
                     /locus_tag="PK348_00655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK64753.1"
                     /translation="MRINNVAIVIYLSVIMALGMAMGMVYSDDNSLFSTLAITPEDVP
                     QGFIFGKIPNFAKKVFTGNPCIVNSDGIRFLASKLYPEGNAANIKGMYVAIMASESRP
                     YGDDLVYYLLVFKDSKAASVEIKKLQDYYQYNKDRIVLITKDNIALLLFADDVDNYKY
                     IAELGQKLQERLN"
     gene            complement(1093..1497)
                     /locus_tag="PK348_00660"
     CDS             complement(1093..1497)
                     /locus_tag="PK348_00660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK64754.1"
                     /translation="MELFDGFIAAIASEFQLKFHKTKEGAYTTTIEFENDRSQEILIT
                     LNKDESGDRIVNYYSVIGTIKNESFELYKFALKLNSTLDYGALALLNDTLILRNSLLL
                     KDCDPQRFMKSLFYIAAKADELEEMLITKNIY"
     gene            complement(1524..2117)
                     /locus_tag="PK348_00665"
     CDS             complement(1524..2117)
                     /locus_tag="PK348_00665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK64755.1"
                     /translation="MRITNTIPVVPQASLATHRKASIQLQQPVESVRVYPSLKKAISA
                     YTIISEAHQLVTMAINISYELIQKAFTPQDHNEILAKIATINNYFTRLSPGSASVPNI
                     PQSDTLQETTKLIEQLGNAAKNNDTASMKAIHETLTSHAQILDNTQKQMYMALSHPAG
                     TDTPDINKIKSAIEQNQNMVFYAHKPLQYEHIQKLLA"
     gene            2605..2766
                     /locus_tag="PK348_00670"
     CDS             2605..2766
                     /locus_tag="PK348_00670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK64756.1"
                     /translation="MISSISCKKKEENRIPREQIQIDGAHDYSLIFKDIIDKEKKEQQ
                     RYKKGPFDE"
     gene            2759..3421
                     /locus_tag="PK348_00675"
     CDS             2759..3421
                     /locus_tag="PK348_00675"
                     /inference="COORDINATES: protein motif:HMM:NF023757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-J related domain-containing protein"
                     /protein_id="HXK64757.1"
                     /translation="MNKLIEIPKESLIAYCLNIPRPVMESVIIREIVNPLCNGHSTLY
                     QKHFSLFHALYNIKNNPDYFNYMVHIHPMQIVIRNYPVNNKCIDYNPLTDSFCGNQTV
                     DGTYCPFHYTFHAPFRGDMIYDPLNEFYSDPYNINYEFSDELKKVYSGFTKYIINRRK
                     VEQALSFFSFSAMEHCNKALLQKRYRELVKKYHPDHYHGDTTMIKEINAHYTLLKEIF
                     VV"
     gene            3584..4729
                     /locus_tag="PK348_00680"
     CDS             3584..4729
                     /locus_tag="PK348_00680"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HXK64758.1"
                     /translation="MKRRAFQIIPVIVLISVVLFVFNNFSCSNSGPSAFGNSSSSPLK
                     GDQETARAIEIQDTFRKIYELYKDRVVFITTEQVVELPPNPFFDDPFFRQFFGVPEQK
                     SRKQKRSGLGSGFVLSSDGYICTNYHVIANVDSVKVKVKNKVYKATIVGYDQRTDLAL
                     LKINPDGKLDPVYLGDSDQVKVGDWAIAIGNPFGLDRTFTVGVISATSRKDVDMMGGS
                     QSHIQTDAAINPGNSGGPLVNINGEVIGINRMIYSQSGGYMGIGFAIPINTAKAVLEQ
                     LKKYKKVKTGYIGVQIVPLTEEYANELGLKNTEGALVGGIMEDSPAEKAGIQPGDVIL
                     SVDEKPIKTYEDLVEIVSKTPIGKTLKIGVWRNKSKIYLFVTVKERP"
     gene            4782..4991
                     /locus_tag="PK348_00685"
     CDS             4782..4991
                     /locus_tag="PK348_00685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK64759.1"
                     /translation="MDNKYRYIVIIAGIVAIVYIVFRYIIPLLFKLLGIIVSFVAYSI
                     AIVIAIAIVILIIGYVARIYKTHTM"
     gene            5333..7735
                     /locus_tag="PK348_00690"
     CDS             5333..7735
                     /locus_tag="PK348_00690"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HXK64760.1"
                     /translation="MKEQIKIWLDKTKNIINVYINDVRTWFYGIIAVSIIGGLLFGYI
                     VAQVQNCLGIDNLKRFQPSVPTKLYDVNGELIAELFQEKRDLVSFDELPKTLIKAFVA
                     TEDQDFYAHIGISPMAMARAMVKNIISFKIVQGGSTITQQLAKRLFTSGERTFTRKAL
                     ELVLTLQIEKRFTKDEILEMYFNQIYLGHGCYGIASAAELFFNKEVRFLSLAEGSVLA
                     ALPSAPGRYSPFMNTRNAYYKNWDILNRMVDCGFLTKQKAETVYTSFWPQYVDAIKTE
                     FPTKTAYSRIIDNAPFFTDYVRQILISRYGKDVVYNEGLQVYTTLNLKRQRAGEKYLT
                     EGLIKQNEVSARANQYMSGAVDRGLFSVYQQLGMLFNLPRVIVKSDTETIFKKFMVDN
                     LLDEMELVSLLCNADAMHNDIEKFHVVTSGISTSLKVEGALIAVEPKTGYITTMIGGS
                     EFAVSNQYNRAIQARRQPGSAFKPFVYGAGIESKLINAAMALPDAPIVDIDAQGSTWS
                     PDNYEGEFSGMVHLRRALAASINVISVRIYDIIGADTIIDFASRVLKVSPTRFHPGPS
                     LALGSSELTPFEMVTGYAIYANRGRDVIPFAIRYITDRDGNEIENIEEDIGNIIAAKE
                     ADGSIQIVPENVNYVMTSLLQSVAESGTPTEALRVVAKFDKKCAGKTGTTQNWTDAWF
                     CGFTPDIAAVVWVGYDKPFMSLGRHQAGAAVAAPIWGNYMREVYNGMPDPQWPAAPKD
                     VFYGATCKYSGCAPGPNCNELISDLMIPGGTPKCVCNGVHYKMKSVFERYMEKEGLKY
                     EE"
     gene            7877..8356
                     /locus_tag="PK348_00695"
     CDS             7877..8356
                     /locus_tag="PK348_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HXK64761.1"
                     /translation="MFKVGDKIVYPLHGVGIIEAIEKKVVLSKRNEFYIITIINSGMK
                     VMIPTEKAESIGLRKVIAKKDVTKVLNLLKKSDVETEDDWKIRYQNNVDKIKSGSIFA
                     VAEVARDLYKRGKDKELSIMERKLYENAYQLMIHEIALAKDIDVDEAGNLVSEALSS"
BASE COUNT         2753 a         1290 c         1733 g         2677 t
ORIGIN      
        1 tacggtgctg ctcacggtac aggatataag cttttgcaac ctttgcatgc cctttttcaa
       61 tgagcatctt ttcaactaaa tcctgaacat tttcaactgt tggtatatgt tcgcctttat
      121 ataaaagtga caatatatca acaaccgatt cagcaatttg agctgcagta tttctatcag
      181 tgccaccaac agcgcgggca gccttgaata ttgcttctgt aatcttttct atattaaaag
      241 gaactatcct accatcccgt tttcttataa actgggggct atcttttgaa tctgtcatat
      301 aaaaactccg taaacggtga attagtatga atggtgaaaa aaactataat gcaatggtta
      361 aggattgctt gctacgggta tttttaaaaa acagtgacat actgaaacaa aaaagtcaat
      421 aattttatgt cacataataa aaataatttg ctatacatat attagtataa atatgtatgt
      481 atattcttta gtcaagaaaa aattaaatgg taaaatttta ttatttttcc gatagtatag
      541 tgatattaat cagtaaaata taaaaggtaa agctatgaga ataaataatg tagcgatagt
      601 tatatattta agtgttatta tggctttggg catggctatg gggatggtat attcagatga
      661 taattcactt ttctcaactc ttgcaataac tcctgaagat gtgcctcaag gctttatatt
      721 tgggaaaata cctaattttg caaagaaggt gttcactgga aatccatgta ttgtgaattc
      781 agatggaata cgatttttag cttccaaatt atatccggaa ggtaatgctg caaatatcaa
      841 gggtatgtat gttgcaatca tggcttctga atcaaggccg tacggagatg atctggtgta
      901 ctatctgctt gtttttaaag atagtaaagc agcttcagtt gaaataaaaa aacttcagga
      961 ttattatcag tacaacaaag acagaatagt cctcattaca aaagacaaca ttgcgctatt
     1021 gctctttgct gatgatgtag ataattataa atatattgca gagttgggac agaaactaca
     1081 ggaaagactc aactaataaa tattttttgt gatgagcatc tcttctaatt catctgcttt
     1141 tgctgcaata taaaatagtg atttcataaa tcgctgtgga tcacaatctt ttagcaaaag
     1201 agaattcctt aaaattaatg tgtcattgag caatgccagg gcgccataat caagtgtact
     1261 gttgagcttc agcgcaaact tatacagctc aaatgattca ttttttatag ttcctataac
     1321 tgaatagtaa tttacaatcc tgtctcctga ctcatctttg ttcagagtaa ttaaaatttc
     1381 ctgagatcgg tcattctcaa attcaatggt tgtggtatat gcaccctctt ttgttttatg
     1441 aaattttaac tgaaattcgg aagcaattgc tgcaataaat ccatcaaata attccataca
     1501 tcacctttta ctgtaataat tttttaggcc aatagtttct gaatatgctc atactgtaat
     1561 ggtttatggg cataaaaaac catgttctga ttttgttcta tagcactttt tattttatta
     1621 atgtctggcg tatctgtacc tgcaggatgg cttaaagcca tgtacatttg cttttgtgta
     1681 ttgtcaagaa tttgtgcatg tgaggtgagt gtttcatgta ttgctttcat tgatgctgta
     1741 tcattatttt ttgctgcgtt tcccaattgc tcaataagct ttgttgtttc ctgcaatgtg
     1801 tcagattgag gaatatttgg aacagaagct gaacctggtg aaaggcgagt aaaataatta
     1861 tttatagttg caattttagc aaggatttca ttgtggtcct gaggtgtaaa tgctttctgt
     1921 ataagttcat aggatatgtt tatagccatg gtaacaagct gatgggcttc gctgataatg
     1981 gtataggccg aaatagcttt tttcagtgaa ggataaaccc gtacagattc cactggttgc
     2041 tgtagttgaa tacttgcttt tctatgagta gccagtgatg cttgtggtac tactggtatg
     2101 gtatttgtta ttctcatagc tgcccccgac cagataaaaa tatatagtat gtatacaata
     2161 atgtcaacaa gtatttattg tggatgtttt ggacatatag gaattactac tttattttag
     2221 gtagccgtat attctgctgt agatacttca atggatgaaa ataacacaag aatggcatgg
     2281 tgacaatagc atgagcgctc tggatggggt atcttagcct gctggagtgt ggccatctct
     2341 gttaaggaaa gaaagtgctt ctttaattta ctttgggata gtaatgactt gatatgctaa
     2401 atttaattta ttaataaaat ggataaaaaa caatattgaa aattaggtaa tggtattgta
     2461 ctgttgtagt aattatgttg tacgtgatta ttgattaaaa aggagttttc aatcatacta
     2521 catccttgcc gtatttggta aagggataaa tattatggta gagtttttat gaaaaaatta
     2581 gtcattatac tctgttgtat atgtatgata agtagcattt catgcaaaaa gaaagaagaa
     2641 aacagaatac cccgtgagca aattcagatt gatggggcac atgattattc tttaatattt
     2701 aaagatatca tagataaaga aaaaaaggaa caacaacggt ataaaaaagg tccctttgat
     2761 gaataagctc attgaaatcc ctaaagagag tttaattgcc tattgtctga atataccacg
     2821 gccagtaatg gaatcagtta tcataaggga aatcgtgaat cctttatgca atggtcattc
     2881 tacattgtat caaaagcatt tttcgctgtt tcatgcatta tacaatataa aaaataaccc
     2941 tgattacttt aactatatgg tacatattca tccaatgcaa atagttatca ggaactatcc
     3001 tgtaaataat aaatgcattg actataatcc cttgacagat agtttttgtg gaaatcaaac
     3061 cgtggatgga acatactgtc catttcacta tacttttcat gcacccttta gaggggatat
     3121 gatatatgat ccgctcaatg agttttacag tgacccgtac aatattaatt acgaattttc
     3181 ggatgaattg aaaaaagtat atagtggctt tacaaaatat attattaatc gcaggaaggt
     3241 tgagcaggct ctatcatttt tttcatttag cgctatggag cattgtaata aggcattact
     3301 acaaaagcgt taccgtgaac tcgtgaagaa gtatcatcct gatcattacc atggtgatac
     3361 aacaatgatt aaggaaatta atgcacacta taccctttta aaggaaattt ttgtagtata
     3421 atactatatg aagtggcatt cataaatatg aatttttttg tagcattttt accagttaac
     3481 atactaatct ggtcatgaaa ggtatttact aaaactattg tatcaaaata aggtaaataa
     3541 tatattataa gcgatagtta ccataaaata aaaggagaat gatatgaaaa gaagagcatt
     3601 ccagattatt ccagtgatag tcttgataag cgtcgtgctt tttgtgttta ataatttttc
     3661 ctgttccaat agcggcccat ctgcgtttgg caatagcagc agttcacccc tgaaagggga
     3721 tcaggaaaca gcacgggcta ttgagattca ggatacattc agaaaaatat atgaattata
     3781 caaggacaga gtggtattta ttactactga acaggttgtt gagttgccgc caaatccatt
     3841 ctttgatgat ccttttttca ggcagttttt tggtgtaccc gagcagaaat cacgcaagca
     3901 aaaacgaagt ggtttgggca gtggatttgt gctatcaagt gatggatata tttgtacaaa
     3961 ttatcatgta atagccaatg ttgactcagt taaggttaaa gtaaaaaata aagtctataa
     4021 agctacaata gttggttatg atcagcgtac cgatttagca ttgctgaaga ttaatcctga
     4081 tgggaaactt gatccggtat atctgggcga ttctgatcag gtaaaggttg gtgattgggc
     4141 tattgctata gggaacccat ttggccttga cagaacattt acggttggag tcataagcgc
     4201 aaccagccga aaagatgttg atatgatggg gggttcccag tcacatattc aaacagatgc
     4261 agcaattaat ccgggaaatt ccggtggacc actggtaaac attaatggtg aggttattgg
     4321 aattaacagg atgatctatt cgcaaagtgg tggttacatg ggaatcggtt ttgctatccc
     4381 tattaataca gcaaaggcgg tacttgaaca gcttaaaaaa tacaagaaag taaaaacggg
     4441 gtacataggt gtacagatag tacctttaac agaagagtat gctaatgaat taggacttaa
     4501 gaatacagaa ggtgcactgg ttggtggtat tatggaagac agccctgcag aaaaggcagg
     4561 gatccagcca ggtgatgtaa tattatcagt tgatgaaaaa cccattaaaa cctatgaaga
     4621 tctggtggaa atcgtaagca aaacaccaat tggcaaaacc ttaaaaattg gtgtctggcg
     4681 aaataaatct aaaatatatc tgtttgtaac tgttaaggaa agaccataaa tgttttatag
     4741 tgtatgcaat taacaataag tgatagataa ctgtatgcat catggataat aaatatcgtt
     4801 acatagtaat aatagcaggg atagtagcga tagtatatat agtatttcgc tatattatcc
     4861 cgctattatt taaattattg ggtataattg taagttttgt tgcttacagt attgctatcg
     4921 tgatagctat agcaattgta atacttatca ttggctatgt agcacgtatc tataaaactc
     4981 atactatgta aaaatagttg acgtatatag aataataaga gggatatcat gcatattcct
     5041 ctttgctaag taaacaatta gtgatgattt caaagcagtg aggaaacctt acaaaatact
     5101 atagtgatag taggggctgt accaaaagag gtcgttgagt aaattttgca aacattatat
     5161 cgtaatactc gctcatttcg atacaatttt gccacaaaat ctggcaaaat tactcaatga
     5221 gcgttacgaa atttgtatcg aaactacttt tgggacagcc cccttgatgt aacaatagat
     5281 tacatttttt gaatgatatg catgaaatca tgttttagac ggagtatata gtgtgaaaga
     5341 acaaataaaa atctggctgg ataaaactaa gaacatcatc aatgtatata tcaacgatgt
     5401 gcgtacatgg ttttatggta ttattgcggt atcaatcatt ggaggactgc tatttggata
     5461 tattgtagct caggtgcaga attgtctggg aatagacaac ctgaaacggt ttcagccaag
     5521 tgttcctaca aaactgtatg atgttaatgg ggaactcatt gccgagcttt tccaggaaaa
     5581 aagagatctg gtgtcttttg atgaacttcc aaaaacgctt attaaggcat ttgtagcaac
     5641 tgaagaccag gacttttatg cacacattgg cataagtcct atggctatgg ctcgggcaat
     5701 ggtaaaaaat attatctcct tcaagattgt tcagggtggt tctaccatta cacagcagct
     5761 tgcaaaacgg ctttttactt ctggagaacg aacattcaca agaaaagcac tggagcttgt
     5821 attaacactg caaatagaaa aacgtttcac caaagatgaa attttggaga tgtactttaa
     5881 ccagatatat ttagggcatg gctgttatgg cattgcatct gcagcagaat tattttttaa
     5941 taaagaagtt aggtttttga gcctggcgga aggtagtgtg ttagctgcgt tgccttcagc
     6001 acctggaagg tattcacctt ttatgaatac acgcaatgcc tattataaaa actgggatat
     6061 tttaaacagg atggttgatt gtggcttttt aacaaagcag aaggcagaaa cagtctatac
     6121 ctctttctgg ccccaatatg tagatgcaat taaaacagaa tttcctacaa aaacagcgta
     6181 ttccagaata attgataatg cacctttttt cactgattat gtccgacaga ttttgatttc
     6241 ccggtatggc aaagatgtgg tatataatga aggacttcag gtgtatacca cactgaattt
     6301 gaagcgtcag cgtgcaggag aaaaatattt aacagaaggc ttaataaaac aaaatgaggt
     6361 atcagcacgg gcaaaccagt acatgtcagg agctgttgac aggggtttat tttctgtata
     6421 ccagcagttg ggcatgcttt ttaaccttcc aagagttata gtaaaaagtg ataccgaaac
     6481 tatatttaaa aaattcatgg tagataacct ccttgatgaa atggagcttg tatcgttatt
     6541 atgcaatgcc gatgccatgc ataatgatat agaaaaattt catgtagtga ccagtggcat
     6601 ttcaacttct ttaaaggttg aaggtgcttt aattgcagtg gaaccaaaga ctggctatat
     6661 caccacaatg attggtggtt ctgaatttgc agtaagtaat cagtataaca gggcaattca
     6721 agcacgacgc cagcctggtt cagcgttcaa gccttttgtg tatggagctg gtattgaatc
     6781 taaactcatt aatgctgcca tggcattgcc cgatgcacca attgtggata ttgatgcaca
     6841 gggaagcacc tggagccctg ataattatga aggtgagttt tccggtatgg tgcatttgcg
     6901 aagagctctt gctgcatcaa ttaatgtcat ctctgtacgt atttatgata ttattggtgc
     6961 tgatactatc atagattttg cttcaagagt attaaaggtt tccccaacac gatttcatcc
     7021 ggggccttca ctggcgttgg gtagttcgga actgactccc tttgaaatgg tgaccggata
     7081 tgcaatatat gcaaaccggg gaagggatgt tataccattt gcaatacggt atattactga
     7141 tagagatggc aatgagatag aaaatattga agaggatatt ggcaacatca ttgctgctaa
     7201 agaagcagat ggatcaatac agatagtgcc tgaaaatgtt aactatgtca tgacttcact
     7261 gctgcaaagc gttgctgaaa gcggtactcc tactgaagca ctgcgcgttg tggcaaaatt
     7321 tgataaaaaa tgtgctggaa aaactggaac aactcagaac tggacagatg catggttttg
     7381 tggatttacg cctgatattg ctgcagtagt gtgggtgggg tatgataaac cttttatgtc
     7441 actgggaagg catcaggcag gtgcagctgt agcagcgcca atatggggca attacatgcg
     7501 tgaagtatat aatggcatgc ccgatccaca gtggcctgct gcacctaagg atgtttttta
     7561 tggggcaacc tgtaaatatt caggatgtgc accgggtcca aattgtaatg agttaatcag
     7621 cgatttgatg ataccaggtg ggactccaaa gtgtgtatgc aatggtgttc attataagat
     7681 gaaatcagtt tttgaacggt atatggaaaa agagggcctg aaatacgaag aatagtattt
     7741 taaaaaaagg caacaatgct cctgcctatc gcataaaaat aaaatatttt attcttgatt
     7801 ttataatttt tgtgtgctac tattaatagc ttgtaggtta tattattgcg ggttaattta
     7861 tatatgaggg tatcctatgt ttaaagtggg tgataagatt gtttatcctt tgcatggggt
     7921 tggcattata gaggctatcg aaaaaaaagt agtattaagt aaacggaatg aattttacat
     7981 tataaccatt attaatagtg ggatgaaggt aatgattccc actgagaaag ctgaatcaat
     8041 agggttacgc aaggttattg ccaaaaaaga tgtaactaaa gtattgaacc tgctgaagaa
     8101 atcagatgtt gaaaccgaag atgactggaa gataaggtat cagaataatg tggataagat
     8161 taaaagtggg tcaatttttg cagtagccga agttgcgcgg gacctgtaca agcgggggaa
     8221 ggataaggag ctttctataa tggaacgaaa actgtatgaa aatgcttacc agcttatgat
     8281 acatgagata gccctggcaa aggatatcga tgttgatgag gctggcaatc tggtatccga
     8341 agcgttatcc tcataaacta cctgcagtac tataacctgt attctcgcaa atatttacgt
     8401 tttattgtat cctgcatcac tacattgtta ttatattatt attaatgtaa tag
//
LOCUS       DAOSZP010000014         8300 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_7330_length_8300_cov_3.485385, whole genome shotgun sequence.
ACCESSION   DAOSZP010000014 DAOSZP010000000
VERSION     DAOSZP010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 8300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8300
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7330_length_8300_cov_3.485385"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1382)
                     /locus_tag="PK348_00700"
     CDS             complement(<1..1382)
                     /locus_tag="PK348_00700"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HXK64762.1"
                     /translation="MQKATLEGYKEFYTNEKSVINMVLSRAKKRGNAAALSGFIDGKK
                     YSYTWKDLTKIVYAIAHFLIAKGLQKGDRVAIFSQNCPEWTLADLGIQAAGCVPVPIY
                     ATNSRDEAKYILDDASIKAIFTGDQEQYDKILQIMNSTSLQFVVSVQDAVIVNGENSF
                     YIHEIVKTEVSDNAKKTVDARINELSSNDMLTIIYTSGTTGNPKGAVHTHASFLAGIY
                     ASVFRFPEAGPGMVSLAFLPLSHVFERMWTYGVLLKGGENHYCRNPKDIMEILPLSKP
                     HYMCSVPRLWEKMYATIFDRLETAPPAKKKLFNWATTIGIEYDTRKRAKQFIGPWLGI
                     KRFFANALVLKKVRQIVGGNVWVFHVGGAAMSAEINAFFNGLGIPLAQGYGLTEFFPV
                     AVGTAATAYPGVCGPILDIVDVRVSDEGEIQLKGPMCMQGYLNKPEATREMFTHDGWF
                     KTGDVGTIEE"
     gene            complement(1437..2513)
                     /locus_tag="PK348_00705"
     CDS             complement(1437..2513)
                     /locus_tag="PK348_00705"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015948588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HXK64763.1"
                     /translation="MYHNKNIHLRRNTMKTKITELFKIKYPIVLSGMSWISTPELVAA
                     VSNAGGLGILATGVLTAEQTREAVRRVRELTNKPFAANVTLYFPGAERNAEVLIEEKV
                     PIINYSLGKGDKIAKAVHAYGGKVIATVTTHKHALAAQRDGADALIVTGYEAAGHGGA
                     ITSLVLIPAISQAVNIPVIAAGGFADGRGLIAALALGAEGISMGTRFMNTKESPVAES
                     AKQASIKSEVYNTVYTPKIDGLPARFMKSKAVERLMKKHLNPLSSLIRSKKIADMLGY
                     PWLKLAVGIMFMGPQRAIQMARMAIGFDAFKIGTMDGDFDKGVLPLGQVTGIINDTPT
                     VKQVVERIIKEAVEIEKQLAKKVK"
     gene            complement(2642..3961)
                     /locus_tag="PK348_00710"
     CDS             complement(2642..3961)
                     /locus_tag="PK348_00710"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HXK64764.1"
                     /translation="MLEVEKLNLSRIQETCAKQKTRWWDVFAEYAVHKTYSFKNNVFY
                     GVRESEIKGYGIRMNIDGKTGFSFCNDIAMLDSVMEYARETARYGEIEEYDLPAKQPT
                     PHVECYDGNIFNKDTQYYLQTLQNVVDTIRSAYSDCMVDAGLGCVDGYIHLINSRGFD
                     SGYHYSRQGSTISALRILPDGSRVQVYESITWNSDVNIHELSERIIQKLNFSKQTVKM
                     PFGNYYVIFTPKAFGQIMGVVLQGLTGRSIERGISRYIGKFGEKVFGRQFTVYDNPLI
                     DFAPGSYPFDDEGIPAKSKTIIENGYVKTYIAHLQSAHLLHVQPTGNASRGYAILPQE
                     SFSNIVVEPGTTSFKDMIAQMGTGIIIDQFIGFGQSNTLMGDFSANLDLAWLVVQGEV
                     KGRLTNSMVSGDVTTMLNDKIVLSSEREWGGDAYLPYVLCRINYSTT"
     gene            complement(3930..5327)
                     /locus_tag="PK348_00715"
     CDS             complement(3930..5327)
                     /locus_tag="PK348_00715"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HXK64765.1"
                     /translation="MLDPMDLQKKLKSIKTEYAEIRISESFSTTIHLSKRMIETCKVG
                     TSTQGSVRVYNKGCWGFFAFTSLDMVDYAMQQALQLSNIQKKENAPTLSSLQMNVIKE
                     KTNVLIPPNTVSLEEKMNVLTQYNDILKDSNLIENTNVLYRDSLTQYVLCNTDGSIVE
                     YDRSFCGLSLSAIAKDGMVIQPYGESDANYAGFEIVKDKHPVAEEVVRTATELVQAPQ
                     IKSGVYNVIADPKLAGVFIHEAFGHLSEADFVYENPRMKEIMVIGKRFGPDILNVVDD
                     GTLPYAGYIPCDDEGIKPEKTYLIKNGILHSRLHSRETAVKMGEEVTGNARAITASAS
                     PIVRMTNTYIENGNSTVEELFEKLWNGVYVVGALGGQTNLEMFTFTPAYAYEVKKGKK
                     TKKLRECMLTGNVFVTLANIEAIANDCTLFGGLGGCGKQGQAPLPVSSGGPHILINNV
                     LIGGKGAGSRKTKSK"
     gene            complement(5590..6303)
                     /locus_tag="PK348_00720"
     CDS             complement(5590..6303)
                     /locus_tag="PK348_00720"
                     /inference="COORDINATES: protein motif:HMM:NF016873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HXK64766.1"
                     /translation="MKNVSIIITCEHAGNRVPEALRDIFTDTTILSTHWAYDIGALQM
                     ARILAKTLEAPLLYTTVTRLVVDCNRSIGSNELFSELIQGSNIFLKNEIVKRYYTVYR
                     NKIEKAINTMLQSSDAVFHIAAHSFTPQLYGFKRYTDIGLLYDQSRPKEALFCSKIKE
                     ALMQAYPLRVAFNYPFRGCGDGVTVWLRKQYGDSYCGIELEANQQIFFNSKRQWRTVS
                     YHLAQAILYAVNNVVQEDY"
     gene            6393..6749
                     /locus_tag="PK348_00725"
     CDS             6393..6749
                     /locus_tag="PK348_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013749653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HXK64767.1"
                     /translation="MLIITTIIGLSAGILGGLMGIGGGIIMIPALIYLLHFNQHMAQG
                     TTLAAMIPPIGILAAYEYYKSGNVNIPVAITLALGFVIGGYIGGKFAVSLDNETLRRV
                     FGIILFIMSLHIIFSK"
     gene            complement(6750..>8300)
                     /locus_tag="PK348_00730"
     CDS             complement(6750..>8300)
                     /locus_tag="PK348_00730"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HXK64768.1"
                     /translation="TRHEQAAVFMAEAWGRLSRKPGVALVTAGPGFTNALTAIANARL
                     SNAPLLLIAGCVGLESNEKLDLQDMKQLPVIEPMVKKAFVCHKPERVYEYVDMAYRTA
                     CSGRPGPVYLELPVDVLNAAPDETKVKKTNTLVYSKPCDSNAAYDVIELLQHKKKPII
                     IAGSGAWYADAARELLQFVESTGIPVFTNAAGRGVIADTHPLCFESSLAIRPGAALYA
                     NFNADLVLLLGNRVSLYYIFGDIFNKDATLVQVDIEPEEIGRNRSIYKGILSDVSSFI
                     DECNKIVKERQLNLPEQFKEWVLELKKADEESKKQAQQQWLSNAVPIHAMRVAYEVNQ
                     IMDKEDDVVVSDGGDAQIWMGMTRTVKKGGTYLDSGLYGCLGVGIPFANAAKLYYKNS
                     RVVLFTGDGSLGFNFMEIETAIRKGLSITIVVSNDLGWGMIRHSQVLRMGHPVKEGTF
                     IGNIPYHKLVEALGGKGYVVEKPEDIKPAIIDALQSNTVALVNVMTDPDTISPGSVAL
                     ANLGGYKA"
BASE COUNT         2363 a         1933 c         1400 g         2604 t
ORIGIN      
        1 tgttcttcaa tggttccaac atcacctgtt ttaaaccagc catcatgtgt aaacatttcc
       61 ctggttgctt caggtttatt taagtaaccc tgcatacaca tggggccttt cagctggatt
      121 tcaccttcat cagaaactct gacatcaaca atatcaagaa ttggaccaca gacaccagga
      181 taagctgttg cagcagtccc aacggcaact gggaaaaatt ctgtcaatcc atacccctga
      241 gccagcggga tgcccaatcc attgaaaaat gcattgatct cagcagacat agctgctcca
      301 ccaacatgga aaacccatac attgccacca actatctgcc gcaccttttt taatacaagg
      361 gcgtttgcaa aaaatctttt tataccaagc catggcccaa taaattgttt ggcccttttt
      421 ctagtatcgt attcaatgcc gatagtagtt gcccaattaa acaatttctt ctttgcaggt
      481 ggcgctgttt ctaatctgtc aaatatggta gcatacatct tttcccataa acggggaacg
      541 ctgcacatat aatgtggttt tgacagtggc aaaatttcca taatatcttt tggattacga
      601 cagtaatggt tttcaccacc tttcagcaat actccatatg tccacatgcg ttcaaacaca
      661 tggcttaatg gcaaaaatgc aagagatacc atacccggtc ctgcttcggg gaagcggaat
      721 accgatgcat agataccagc caggaagctt gcatgcgtat gtaccgcacc tttaggattc
      781 ccggttgttc ccgaagtgta gattatggta agcatgtcat tgctcgataa ctcattaata
      841 cgagcatcaa ctgttttttt tgcgttatca ctaacttcag tttttactat ctcatgaata
      901 taaaaactat tctcaccatt tactatcaca gcatcctgaa cagatacaac aaactgtaag
      961 ctggttgagt tcattatctg aagaatctta tcatactgtt cctgatcacc agtaaagatt
     1021 gctttgatag aagcatcatc aagtatatac tttgcctcat ccctggagtt agttgcataa
     1081 attggtaccg ggacacagcc tgctgcctga attcctaaat cagctaatgt ccattcaggg
     1141 caattttgtg aaaagattgc aaccctgtca cccttttgca ggcctttagc aattaaaaaa
     1201 tgagctatcg catacactat ttttgtcaga tctttccagg tatagctata cttcttgcca
     1261 tcaatgaatc ccgatagtgc ggctgcatta ccacgtttct ttgcacgtga taacaccatg
     1321 ttgataacac ttttttcatt ggtataaaat tccttatatc cttcaagtgt tgctttctgc
     1381 atagataccc caccccatga taaattttta ttagctcctg taaaaaacga tggctactat
     1441 ttaacctttt ttgccagttg cttttcaatc tccactgctt ctttgattat tctttccaca
     1501 acctgtttca ctgttggtgt atcattaata atgccggtaa cctgacccaa tggcaaaaca
     1561 cctttatcaa aatcaccatc catggttcct atcttgaatg catcaaaacc aattgccatg
     1621 cgtgccatct gaattgctcg ttgtggtccc ataaacataa tgccaactgc aagttttaac
     1681 catggatagc ctaacatatc agcaattttc tttgaacgaa taagagagct cagtggatta
     1741 agatgctttt tcatgagtcg ctcaacagcc ttagatttca taaatcgtgc aggcaatcca
     1801 tcaatttttg gagtatacac ggtgttgtat acttcactct taatacttgc ctgctttgca
     1861 ctttcggcca ccgggctttc cttggtattc ataaagcggg tgcccattga tatgccttct
     1921 gctcccagtg caagagctgc aataagcccc ctgccatctg caaaaccacc tgctgcaata
     1981 acaggaatat ttactgcctg tgatattgca ggaatcaaca ccagcgatgt tatggcacca
     2041 ccatggcctg ctgcttcata gccggttact atcagggcat cagcaccatc gcgctgggct
     2101 gccagtgcat gtttatgcgt ggtaactgtc gcaattactt ttccaccgta tgcatgaaca
     2161 gcttttgcaa tcttatcacc tttacccaat gaatagttta tgataggaac tttttcctca
     2221 atgaggactt cagcgtttct ctctgcacca ggaaaataca gcgtaacatt tgctgcaaat
     2281 ggtttattgg taagttcccg tactcttcgt actgcctcac gggtctgttc agctgttaac
     2341 acacccgttg caaggatacc aagacctcca gcatttgaaa ccgcagcaac aagctcaggt
     2401 gtacttatcc agctcatacc tgaaagcacg attggatatt tgatcttgaa aagttctgta
     2461 atttttgttt tcattgtgtt tctcctcaaa tgtatattct tattatgata cattatttcc
     2521 gctaaaagcg gatgaattat accttgttat ctatctcctc ttattctatc gcaaacataa
     2581 cagagttatc taaaaaagca agaaatatat atactttttg tcaaaacttt tatataaaaa
     2641 attatgtagt tgaataattt atacggcaca atacataggg gagataggca tcgccacccc
     2701 attcccgttc tgaagaaagt actattttat cattcaacat tgttgtaaca tcaccactta
     2761 ccatggaatt tgttaatcgt cctttgactt caccctggac aaccagccat gccaggtcca
     2821 gattggctga aaagtctccc attaatgtat ttgattgacc aaagccaata aactgatcta
     2881 taataattcc tgttcccatt tgagctatca tatccttaaa ggacgtggtg cccggttcaa
     2941 caactatatt gctaaaactt tcctggggta agatagcata ccctcgtgaa gcattcccgg
     3001 taggttgtac gtgcaaaaga tgggcactct gcaaatgagc aatataggtc ttaacatacc
     3061 cattttcaat aatagtttta cttttagctg gaattccctc atcatcaaaa gggtaactac
     3121 caggtgcaaa atcaattaac ggattatcat aaactgtaaa ctgtctacca aaaacctttt
     3181 ctccaaattt tccaatatat cgtgaaattc cacgctcaat tgaacgcccc gtcaaaccct
     3241 gcagaaccac acccattatc tgaccaaacg cctttggagt aaaaattaca taataattac
     3301 caaatggcat tttgacggtt tgtttgctaa aattcagttt ttgtataatt cgttcagaaa
     3361 gctcatgtat attaacatca ctgttccagg taatactttc atatacctgg acacggctac
     3421 catcaggcaa tattcgcaag gcagatattg ttgaaccctg ccgcgaatag tggtacccac
     3481 tatcaaaacc cctggaatta atcagatgta tatatccatc cacacacccc aagcccgcat
     3541 caaccataca gtcactgtat gctgaacgaa ttgtatccac aacgttctgt aatgtttgga
     3601 gatagtattg ggtgtcttta ttgaatatgt tcccatcata gcattcaaca tggggagtgg
     3661 gttgttttgc cggaagatca tattcttcaa tttcaccata acgtgctgtt tcacgtgcat
     3721 attccatcac agaatcaagc attgcaatat cattacaaaa tgaaaaaccc gttttgccat
     3781 ctatattcat cctgatgccg tatcccttta tttctgattc acgcacacca taaaatacat
     3841 tatttttaaa tgaatatgtt ttatgtactg catactcagc aaatacatcc caccagcgag
     3901 ttttttgttt tgcacaggtt tcctgtattc tacttagatt tagtttttct acttccagca
     3961 cctttgcctc ctataagaac attgttaatc aatatatgcg gcccaccgga agaaactggc
     4021 agtggagcct gaccctgttt gccacaaccg cccagtccac caaacaatgt acaatcgtta
     4081 gctattgctt caatgttggc aagagtcaca aatacattcc ccgtaagcat gcactcacgt
     4141 aacttcttgg tttttttacc tttttttacc tcatacgcat aggcaggcgt aaaggtaaac
     4201 atttctaaat ttgtttgacc tcccagagca ccaaccacat agacaccatt ccacagcttt
     4261 tcaaataatt cctcaacggt agaattccca ttttctatat acgtatttgt catacgcaca
     4321 ataggactgg ctgaagccgt tatggcacgt gcattgccgg tcacctcttc ccccattttt
     4381 accgctgttt cccgtgaatg aagacggctg tgtaaaattc cattttttat taaatatgtt
     4441 ttttcaggtt ttataccctc atcatcacag gggatgtatc ctgcatacgg aagtgtccca
     4501 tcatctacaa cattcagtat atccgggcca aaacgcttac caataaccat tatctctttc
     4561 atccgtggat tttcatatac aaaatctgct tcagacagat gaccaaatgc ttcatgaata
     4621 aaaacaccag ctaattttgg atcggctata acattatata caccactttt tatctgtggt
     4681 gcctgtacca gctccgttgc tgtacgcaca acttcttctg ccaccgggtg tttatccttt
     4741 actatctcaa agcctgcata gttagcatcc gattccccat atggctgaat gaccatccca
     4801 tcttttgcaa tagccgatag cgacaatcca cagaaagacc tgtcatattc tacaatgcta
     4861 ccatcagtat tacataacac atactgtgtc agtgaatcac ggtacaacac attggtattt
     4921 tctataagat tactatcctt caatatgtca ttgtactggg taagcacgtt catcttttct
     4981 tctaagctta ctgtatttgg cggaataagc acatttgttt tttctttaat cacattcatc
     5041 tgtaatgagg atagtgtggg tgcgttttct tttttttgta tgttcgatag ctgtagcgcc
     5101 tgttgcatgg cataatcaac catatcaaga gaagtaaatg caaaaaatcc ccaacacccc
     5161 ttattataaa cgcgaaccga cccctgcgtt gaagtgccta ccttacaggt ttctatcatt
     5221 cttttagaaa ggtgtattgt ggtagaaaaa ctttcactta tacgaatttc agcatattct
     5281 gttttaatgc tttttaactt tttctgtaaa tccattggat caagcatggt aacaatctcc
     5341 attcaataaa aaatctatag tttttttcat caggcaacta aagaaaacaa aatataaaag
     5401 cgtattgctt tgccatctct aaaatctttg cctttgataa aagatgtaat tcaaaatgct
     5461 atcctagaaa atccctgcat gttgggatga taaaaacgtc cctcccataa aaataaagat
     5521 tcttgcccca attttatccc cgattttatc agggtcagaa tgataacaat tgtttaattt
     5581 ttaaaaagct tagtaatcct cctgtactac attattcact gcatacagta ttgcctgtgc
     5641 aagatgatat gatactgtgc gccactgtct tttactgttg aaaaaaatct gctggttagc
     5701 ttccagctca attccgcagt aactatcgcc atactgcttg cgcaaccaca cagtcacgcc
     5761 atcaccgcat cctctaaatg gataattaaa tgcaacccgc aaaggatatg cttgcatcaa
     5821 cgcttcttta atcttgctgc agaatagcgc ttctttaggg cggctctggt catacagcaa
     5881 tcctatatca gtatagcgtt taaaaccata taattgcggc gtaaatgaat gtgctgcaat
     5941 atgaaataca gcatctgaac tctgtagcat ggtgttaata gctttttcaa ttttattacg
     6001 atatacagta taataacgct taactatctc attttttaaa aaaatattgc tcccttgtat
     6061 caattctgaa aaaagttcat tgcttcctat tgacctgtta caatccacca ctaaccgtgt
     6121 aacagtggta tacagcagag gtgcttcaag cgtctttgca aggatgcgtg ccatctgcaa
     6181 tgcgccaata tcatacgccc agtgagtgga aaggatggta gtgtcagtga aaatatcgcg
     6241 caatgcctca gggacacgat tgcctgcatg ttcacaggtt attatgatgg aaacgttttt
     6301 catcagtgcc tcaaaatttt tatataactt gacattatac catactatac gttttctttg
     6361 gtcaatatat attttttaca ggagtagtat ctatgttaat cataaccact atcattggat
     6421 tatcagctgg tattctagga ggattaatgg gcattggggg tggcatcatc atgatccctg
     6481 cacttatata tttacttcat tttaatcagc atatggcaca gggcacaaca ttagctgcca
     6541 tgatacctcc cattgggata ctggcagcat atgaatatta caaatctggc aatgtcaata
     6601 tacctgtagc tataacacta gccttagggt ttgttattgg tggctacatt ggtgggaaat
     6661 ttgctgtatc ccttgataac gaaacattgc gccgtgtgtt tggtataata ctttttatta
     6721 tgtcgttaca tataatcttc agtaaataat tacgccttat atccacccaa gttagctaat
     6781 gccacactgc caggacttat tgtatcagga tcagtcatga cattgaccag cgccacagta
     6841 ttgctttgca atgcatcaat gatagctggt tttatgtcct ctggtttttc aactacatat
     6901 cctttgccac ctaaagcttc taccagctta tgatagggaa tatttccaat aaatgttcct
     6961 tcttttacag ggtgccccat ccgtaaaacc tggctgtgcc gaatcatccc ccaaccaagg
     7021 tcattgctaa caactatagt aatggaaagt cccttgcgta tagcggtttc tatctccata
     7081 aagttgaatc ctaaagaacc atcgccagta aaaagcacaa cacgtgagtt cttgtaatac
     7141 agctttgcag cattggcaaa tggaataccc actcccaaac acccatacaa tcccgaatcc
     7201 aagtatgttc cacctttttt taccgtccgt gtcattccca tccatatctg agcatctcca
     7261 ccatcactga ccactacatc atcttcctta tccatgatct gatttacttc atatgcaaca
     7321 cgcatggcat gaataggcac tgcattactc agccattgtt gctgtgcctg ctttttgctt
     7381 tcttcatcag ctttttttag ttcaagtacc cattctttga attgctcagg taaattcaat
     7441 tgtctttctt ttactatttt attacattca tcaataaatg aactgacatc acttagtatt
     7501 cccttatata tactgcggtt tcgtcctatc tcttctggtt caatatctac ctgaaccagc
     7561 gttgcatctt tgttaaagat gtctccaaat atataatata agctaactct attacccagc
     7621 aataacacaa gatctgcgtt aaaattggca taaagagctg ctccggggcg tattgcaagg
     7681 gatgattcaa aacacagcgg gtgtgtatct gctattacac ctcttccggc agcgttagtg
     7741 aagacaggga tacccgtact ctctacaaat tgcagcaatt ccctggcagc atcggcatac
     7801 catgcaccgc tgccggcaat aataataggc tttttcttgt gctgcaatag ctcaattaca
     7861 tcatacgctg cgttgctatc gcacggtttt gaatatacca atgtatttgt cttttttact
     7921 tttgtttcat ctggcgctgc gttgagaaca tcaacaggca attctaaata caccggtcct
     7981 ggcctgcccg aacacgctgt gcgatatgcc atatcaacat attcgtacac acgctcaggt
     8041 ttatgacaca caaaagcctt ttttaccatg ggttcaatta ccggcaactg cttcatatcc
     8101 tgtaaatcaa gcttttcatt gctttccagc cccacacatc cagcaatgag taagagtgga
     8161 gcattggata accgtgcatt ggcaatagcg gtaagagcgt tggtaaaccc cgggccggct
     8221 gtaacaaggg caactccagg ctttctactc aagcgccccc acgcttcggc cataaataca
     8281 gccgcctgtt catgacgggt
//
LOCUS       DAOSZP010000015         8255 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_7379_length_8255_cov_3.189024, whole genome shotgun sequence.
ACCESSION   DAOSZP010000015 DAOSZP010000000
VERSION     DAOSZP010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 8255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8255
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7379_length_8255_cov_3.189024"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="PK348_00735"
     CDS             complement(<1..222)
                     /locus_tag="PK348_00735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK64769.1"
                     /translation="MSKVIDFLKNEWNSYPRNIQISLYVLFALVALFVIFAIQILFWG
                     LGYTDMNNRFAMGIWIIGDLGFVALGGGAF"
     gene            complement(247..1077)
                     /locus_tag="PK348_00740"
     CDS             complement(247..1077)
                     /locus_tag="PK348_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HXK64770.1"
                     /translation="MAKMTRRWGMVIDLDKCTGCGACNIACAQENNLPIFKDDSDVPK
                     RVTFLDLIEVTNDKDPKAQYPDVKVAYIPKMCQQCSGNDPNNPHPPCVSVCPVVATDV
                     GEDGVVSQIWSRCIGCRYCQVACPYEARVFHWWKPRYEGSFKESLNPDVSIASRGTMV
                     KCTFCSHIWKRERDKAIAQGITDINAVTYTPACASACPTNAILFGDLNDPSSPVYEPK
                     IQKDPRAVRLVHSIDELRTKDPSEYEKRTKIKEYPNPKVYYLTSQQWLRDKISGFKNS
                     "
     gene            complement(1091..3058)
                     /locus_tag="PK348_00745"
     CDS             complement(1091..3058)
                     /locus_tag="PK348_00745"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HXK64771.1"
                     /translation="MKLERKDFLTMIGGAVVGTGVGTLISPGPWMGLQWLVEWTQDQY
                     RPPKGEEKYLASICDQCPNKCTVQVRMIGDRAVKIETSNQGCAAGQLLLQVLYHPERV
                     KTPLKRTGKKGKIDFEPVSWENAIADISKKINDAINNKKQDQIVAINPAFPDATAMLN
                     ERIALFAGSPHVYQESGYWALSSACSLATQKTEGAIDYDFENADCVVSFGARLFEGWG
                     NPGRMHNVLNIWKQKGVKFIQVDSIATRTASLADQWVAIKPGSEPIFAMGVAHYLLKY
                     GRRSGATNFGRFTELLINKFTPEKVSELTGASLETIESVAKALLQARRPVAVAGKGGK
                     VVSSSVAEFAIIQTINSMIGSLGNAVKLVTFATPAKPVIAKKSKGLDEYIASDMTCDI
                     VLINNANPVYSSVYGERLSEKLKKASTVVLFTSLLNDSAQYADYIFPTLTSLEIPFTG
                     QAVSAKFEAKHAGDSLLAIAKNIPNIAAQFPWNSYLDVVKAVNFQAKNVANLDIPVDI
                     ITAKLEELEKMLQASGSYSISMVPFEIPVVGEGNSLALPYVLKALPNTILKGDTSWVW
                     MNPVTASKEGISEGSKIDIESSRGELDTVRVHLTKRVAYDTVAMPIGFGHEELTEYGS
                     EKGVNPKPIMSDALDPLSGYADWWLTRVKLS"
     gene            complement(3104..3622)
                     /locus_tag="PK348_00750"
     CDS             complement(3104..3622)
                     /locus_tag="PK348_00750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HXK64772.1"
                     /translation="MKGTRSFLLFFLPFVIFAYISFIVFQQACDYAMNVQAKKLLPFN
                     HRTHTTTYGASDCEICHSYDSNGRFNGIPTVAVCKQCHDPNKANTQVFFAGFGDNDKP
                     WESFAQQPDLVYFSHIAVMKNEKKAQCISCHGDKGNSTTTAKIKGKMAMGTCMDCHTA
                     LKISNACTVCHD"
     gene            complement(3769..6186)
                     /locus_tag="PK348_00755"
     CDS             complement(3769..6186)
                     /locus_tag="PK348_00755"
                     /inference="COORDINATES: protein motif:HMM:NF014615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HXK64773.1"
                     /translation="MNFRERLYKPYPLVMDGAMGTMIFNALPGYKGNLEILSIERPDV
                     IASIHKQYIDAGADIIETNTFGGNALKLHEAGLDSRCEEINKAAAMLAKDVARGQAFV
                     AGSVGPTGTLVEPMGITSAETVYNAFKRQISGLVAGGVDCIAIETMTDLQEARLALLA
                     AKDVTDIPVICSMTFEQNSKTVTGTDIVTALATLSQMGADIVGANCSMGPDGLVALFK
                     GTIDQLNELGVPLSVWANAGLPELIDGKAVYSLSPKRFASLSLEFAQMGFTIIGGCCG
                     TTPEHIRMLSTAVKHILINTKKSKTFHYITSRYTHINVVNHTGLLIIGERLNPTARKQ
                     FAAELKENTFTFLRNESKKQQDEGAHCLDINVGVPGIDEVAMMKKAVNVLSNTVTIPL
                     MIDSDNPYVTQAALWQYPGIPIINSISAKKQSFETMLPLLKKFGSFVVAMCLDESGIH
                     KEASKRIAIGEKLIDLLQHHGIALSRIFIDPLILAESAESGAAQETLKVIEHFAKKGI
                     KTSIGLSNISFGLPQRKFINNVFLKLALQRGLYAAIVNPGAIDTNTAKPYSTEELLAL
                     DFLEGRDKNASHYIAHFGTGQQQTQQKEKTISILDRIFELVVAGNTDDIEPAVKEALS
                     QHTPETIMNDALITALEKVGQLYSNGEYFLPQMIASADTMKKGFNLLKPLMLQKKESV
                     LGKIVICTVKGDIHDIGKNIVAMMLENHGFDVIDLGKDVPNEKVIEAIQQHKPDIVCL
                     SSLLTTTMPNMGTIAHEIKNQKLPCKLMIGGAVVTKEYADGIGAFYGKDAVEAVAVAK
                     GIVKKSQ"
     gene            complement(6307..7047)
                     /locus_tag="PK348_00760"
     CDS             complement(6307..7047)
                     /locus_tag="PK348_00760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HXK64774.1"
                     /translation="MKKVLNALIEILFFFYRPIFYLLVIIDTTILGILTITLSFFDPT
                     GNTIHYIGVFWSRLNLFLSGVRVRVHNREYIKKDQPYIVMMNHQSHYDVWAAIGYIPL
                     QLRWVMKMELRKIPIFGLGCERMGHIYIDRGDSHQARESLKAAGEKIRNGASVVFFPE
                     GTRSPDGKLLPFKKGGFVIAIESRVPILPITGLGSRAILPKGSMAVRPGTIDLIIHPP
                     IEVTGYTQENKEELMEKVRAVIQSKLVL"
     gene            complement(7055..>8255)
                     /locus_tag="PK348_00765"
     CDS             complement(7055..>8255)
                     /locus_tag="PK348_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HXK64775.1"
                     /translation="DSEVAKRYKNAGLVILGKTNTPEFGLVAYTEPEIFGPTRNPWNT
                     ALTPGGSSGGSAAAVASGMVPFAGGGDGGGSIRIPSAYCGLFGLKPSRGRVPTGPEYG
                     EIWQGAAVEHVLTRSVRDSAAMLDILQGSDPGAPYIIEPPKMPYSKEIQKAPGKLKIA
                     LSTDSPIGSPVHPECKNAALEAAKLCEKLGHYVDIDKPNIDGLALAKSYLMLYFGEVA
                     AEIRTIGIKLGRKPKRSDVEVTTWTLGLLGRTYTAGDFVMALREWNKAARAMGQFFTK
                     YDIYLTPTTAQPPAPIGSLAPKPIELFGSRIVNIFGLGKLAIASGVVDKLAVQNLAQV
                     PFTQLANVTGIPAMSVPLHWGPDGLPYGVQFMAAFGQEALLLRLAAQLEKAKPWFNKR
                     QELYHRG"
BASE COUNT         2322 a         1765 c         1593 g         2575 t
ORIGIN      
        1 gaaggctcca ccgcccagcg caacaaaacc taaatcaccg attatccata tccccatggc
       61 aaagcggtta ttcatatcgg tgtaacctaa tccccaaaac aggatctgaa tagcaaaaat
      121 tacaaacagt gctaccagtg caaagaggac atacagagat atctgtatat tcctggggta
      181 actattccat tcatttttta gaaaatctat tactttgctc acggtatact ctccttatac
      241 ttttttttag gagtttttaa atccagatat tttatcacga agccattgct ggctggttaa
      301 ataatatact tttggatttg gatattcttt gattttagtc cgtttttcat attcagacgg
      361 atcttttgta cgtaattcat ctatggaatg taccagacgt acagccctgg gatccttctg
      421 tattttaggt tcatatactg gcgaagaagg atcattgaga tcgccaaata gtatggcatt
      481 agttgggcat gcacttgcac aggctggagt gtacgttact gcgtttatat cggttatacc
      541 ctgtgctatt gctttatcac gttcacgctt ccagatatgt gaacagaagg tacattttac
      601 catagtaccc cgagatgcta tagaaacatc aggattaaga gattctttga aagacccttc
      661 ataacggggt ttccaccagt ggaatacacg tgcttcgtat ggacaggcta cctgacaata
      721 tctgcagcct atacatcgtg accatatctg gcttacaaca ccatcttcac ctacatcagt
      781 tgcaacaacc ggacatactg aaacacatgg tggatgtgga ttatttgggt cattccctga
      841 acactgctga cacatttttg gtatatatgc cacttttaca tcaggatact gagctttggg
      901 atctttatca ttggttacct caataaggtc aaggaacgtg acccttttgg gcacatcaga
      961 atcatcttta aatattggga gattattttc ctgtgcacaa gcaatattgc atgcaccaca
     1021 tccagtacat ttatctaaat ctataaccat tccccatctt cttgtcatct tggccataat
     1081 taatcacctc ttaactcaat tttactcgtg ttaaccacca gtcagcataa ccactgagag
     1141 ggtccaatgc atctgacatt atgggttttg ggttaacacc tttttcgctg ccatattcgg
     1201 ttaattcctc atggccaaaa ccgataggca ttgcaacagt atcatacgca acgcgtttgg
     1261 ttaaatgtac tctcactgtg tcaagttcac cgcgggaaga ttcaatatcg attttggagc
     1321 cttcggaaat tccttctttt gatgctgtaa ctggattcat ccatacccag cttgtatcgc
     1381 cttttaaaat tgtatttggg agggctttta atacataggg aagtgcaaga ctatttccct
     1441 cgccaactac tggtatttca aaaggtacca tcgatatgga atatgagcct gaagcttgca
     1501 gcattttttc taattcttcc agctttgcag taatgatatc aacagggata tccagatttg
     1561 ctacattttt agcctggaaa tttacagctt ttaccacatc taaataacta ttccatggga
     1621 attgagcagc tatattgggt atattttttg ctattgcaag caatgaatcg ccagcatgtt
     1681 ttgcttcaaa cttagctgat acagcctgac ctgtaaaagg aatttcaagc gaagtaagtg
     1741 ttgggaaaat ataatcagca tattgtgcag aatcattaag taaagaagta aacagcacaa
     1801 cggttgaagc ttttttaagc ttttcagata ggcgctcacc atatacgctt gaataaacgg
     1861 gattagcatt gtttatcagc actatgtcac aggtcatatc gcttgctata tactcatcaa
     1921 gtcccttgga cttctttgct attactggct ttgcaggagt tgcgaaggta acaagcttaa
     1981 cagcgttacc aagactgcca atcatgctgt tgattgtctg tattattgca aattcggcaa
     2041 ctgaagaaga tacaactttt ccgcctttgc cagcaactgc tacaggcctt cgtgcttgca
     2101 gtaatgcttt tgctactgat tcaattgttt cgagagatgc accagttagc tcagaaacct
     2161 tttctggagt gaatttattg attaatagct ctgtaaatct gccaaaattt gttgcacctg
     2221 agcgcctgcc atatttaagc agataatgtg caacgcccat agcaaaaatt ggttcactgc
     2281 caggttttat tgcaacccac tggtctgcta atgaagctgt acgtgttgca atggaatcca
     2341 cctgtataaa tttgacacct ttttgtttcc agatattcaa tacattatgc atcctacctg
     2401 gatttcccca tccttcaaaa agacgtgcac caaagctaac aacgcaatca gcattttcaa
     2461 aatcatagtc gatagctcct tcagttttct gggttgccag tgaacaggca ctggagagag
     2521 cccagtatcc tgattcctgg tatacatgag gactaccagc aaaaagtgct attctttcgt
     2581 ttaacattgc tgttgcatct gggaaagcag gatttattgc tacaatttga tcctgttttt
     2641 tgttattaat ggcatcattt atttttttgg agatatctgc tattgcattt tcccatgaaa
     2701 ccggctcaaa atcaattttt ccttttttac cggttcgttt taatggggtt tttactcgtt
     2761 cagggtggta aaggacctgc aaaaggagct gtcctgcagc acaaccctga ttggatgttt
     2821 cgattttaac agctctatcc ccaatcattc ttacctgtac tgtacattta ttggggcact
     2881 ggtcacaaat acttgctaaa tatttttctt cacctttagg agggcgatac tggtcctggg
     2941 tccattcaac cagccattga agacccatcc atggaccggg tgaaatcagt gtaccaacac
     3001 cagtacctac tactgctcca cctatcattg ttaagaaatc ttttcgttcg agtttcatgg
     3061 atgtaataac tccttgcttg aatttcgatt caataataag aatttaatca tgacatactg
     3121 tacatgcatt gctgattttt aaagctgtat ggcaatccat acatgtaccc atagccattt
     3181 tacccttaat tttagcagtt gtagtagaat tgcccttatc accatggcat gagatacact
     3241 gtgctttctt ttcatttttc atcacagcaa tgtggctgaa atataccagg tcgggctgct
     3301 gggcaaaaga ttcccatggt ttgtcattat caccaaaacc agcaaaaaac acttgggtat
     3361 ttgctttgtt tggatcatga cactgtttac aaactgccac cgtagggata ccattgaatc
     3421 ttccattgct atcataactg tggcatattt cacagtctga agcaccatat gtggtagtat
     3481 gtgtgcgatg attgaatgga agaagtttct ttgcctgtac attcatagca taatcacagg
     3541 cctgctgaaa tactataaat gagatgtatg caaatataac aaaaggtaag aaaaagagta
     3601 gaaaagaacg tgtccctttc acagacgtgc tccttaatct ttgttgtaat gtaaaaacct
     3661 gtaaaaatat ataataaaag aagcggattt gtactacaat ccactataat aacataagcg
     3721 aattcttgta tatacaatct ttatgtcaac taaatttata aaaaaaagtt attgagattt
     3781 ttttacaata ccttttgcca cagcaacagc ttcaacagcg tctttaccat aaaaagcacc
     3841 aatgccatca gcatattctt tagtcacaac cgcaccccca atcatcagtt tacatggcag
     3901 cttctgattt ttaatttcat gtgcaatagt acccatatta ggcatggtag ttgttaaaag
     3961 cgagcttaaa cacacaatgt ctggtttatg ctgttgtatt gcttcaataa ccttttcatt
     4021 tggcacatct ttgcctaagt caatcacatc aaagccgtga ttttcaagca tcatggcaac
     4081 gatatttttc ccaatatcgt gtatatcacc tttaacggtg cagataacta tctttcccag
     4141 cactgattcc ttcttttgca gcatcagtgg ttttaaaaga ttaaaacctt ttttcatggt
     4201 atcagccgag gctatcatct gtggcaggaa atattcgcca ttgctgtaga gttggccaac
     4261 cttttctagg gcggtaatga gtgcatcatt cataattgtt tcaggagtgt gctggctcaa
     4321 tgcttctttt acggctggtt caatatcatc ggtattgcct gcaacaacaa gttcaaatat
     4381 tctatcaagt atagatatag ttttttcctt ttgttgcgtt tgctgctgac ctgttccaaa
     4441 atgtgcaata tagtgtgaag cattcttatc tctaccttca agaaaatcca gtgcaagcag
     4501 ttcttcggta gagtaaggtt ttgcagtatt ggtgtcgata gcgcctggat taacgattgc
     4561 agcatagagg cccctttgta aagcaagctt caaaaatacg ttattgataa atttgcgttg
     4621 tggcaagcca aacgatatat tgcttaaccc aatgcttgtt ttgattccct tttttgcaaa
     4681 atgttcaata actttcagtg tttcctgtgc tgcgccgctt tctgcgcttt cggctaaaat
     4741 aagcgggtca ataaaaatgc gtgacagggc aattccatga tgttgcagta aatcaatgag
     4801 cttttcgcca attgcgatgc gcttgcttgc ttctttatga atgccgcttt catccaggca
     4861 cattgctacc acaaagctac caaatttctt taaaagtgga agcatagtct caaagctttg
     4921 tttctttgca ctgatagagt taataattgg tattcctggg tattgccata aagcagcctg
     4981 agtcacataa ggattatccg aatcaatcat gagggggata gttactgtat tgcttaacac
     5041 gtttactgct tttttcatca tggcaacttc atcaatgccc ggaacaccaa cattaatatc
     5101 caggcaatgt gctccttcat cctgctgttt tttggattca ttacgcagga aggtaaatgt
     5161 gttttcttta agttcagcag caaattgttt gcgtgcagtt gggtttaacc gctctccaat
     5221 gataagcagt cccgtatgat tcacaacgtt aatatgtgta taacggcttg taatgtagtg
     5281 gaatgtttta ctcttttttg tattgataag aatatgtttt actgcagtgg atagcatcct
     5341 gatatgttca ggcgtggtgc cacaacaacc gccaatgatt gtaaagccca tctgtgcaaa
     5401 ttcaagtgac agtgatgcaa aacgctttgg tgaaaggcta tacacagctt ttccgtcaat
     5461 aagctcagga agccctgcat tggcccatac tgaaagaggt acgcccaatt cattaagctg
     5521 gtcgatagtt cctttgaaaa gtgcaaccag gccatctgga cccatgctgc aattagctcc
     5581 aacaatatcc gcacccatct gtgaaagtgt ggcaagtgca gtgactatat ctgttccggt
     5641 tactgtttta ctgttttgtt caaacgtcat gctgcagata acaggaatat cggtaacatc
     5701 ttttgctgcc agaagtgcaa gccgtgcttc ctgtaaatca gtcattgttt caatagcgat
     5761 acaatcaaca ccaccagcta caaggccact gatctggcgt ttaaatgcgt tatatacagt
     5821 ttctgcggat gtaattccca ttggttcaac taacgttcca gtaggaccaa ccgaaccagc
     5881 tacaaacgcc tgaccacgtg ccacatcctt tgctaacata gcagcagctt tattaatttc
     5941 ttcgcatcga ctgtctaaac cagcttcatg gagtttcaat gcatttccgc caaacgtgtt
     6001 ggtttcaata atatcagcac ctgcatcaat atactgtttg tgtattgatg ctattacatc
     6061 aggacgttct atagaaagaa tctcaagatt tcccttataa ccaggcaatg cattgaaaat
     6121 cattgtgccc atagcaccat ccatgacaag tgggtagggc ttatataatc gttctctgaa
     6181 gttcactgct gtataccgtc cttttttcac atgtgatttg aaaaaataac cccttataac
     6241 gcaaggggtt attataatga actatcgcac aataaaaaaa caaaacaaat tttacaacac
     6301 aattttttat aatacaagct tgctctgaat tacagctctg actttttcca tgagttcttc
     6361 tttgttttcc tgggtataac cggtaacttc aattggtgga tgaataatga ggtcgatagt
     6421 tcccggacga acagccatac ttcctttggg cagtattgct cgagatccta atccagtgat
     6481 aggaagaatg ggcacccgag attcaatggc tataacaaag ccgccttttt taaaaggcaa
     6541 aagtttgcca tcagggctgc gtgttccttc cgggaaaaaa accactgatg caccgttacg
     6601 aatcttttca ccggcagctt taaggctttc gcgtgcctga tgtgaatcgc cacggtcaat
     6661 gtaaatatga cccatgcgtt cacatcctaa tccaaagata ggaatttttc gcaactccat
     6721 tttcatgacc catctgagtt gtaacgggat atagccaatg gctgcccaca catcgtaatg
     6781 gctttgatga ttcatcataa caatgtaggg ctgatctttt tttatgtact ctcggttatg
     6841 tacacgtaca cgtactccgg aaaggaaaag attaaggcgt gaccagaata ctccaatgta
     6901 gtgaatggta ttacctgtag gatcaaaaaa tgaaagtgtt atggtaagaa taccaagtat
     6961 agttgtatct ataatgacca gaaggtaaaa tattggccgg tagaagaaga atagtatttc
     7021 aataagtgca ttcaaaacct ttttcatgat gatattaccc cctatgatat agttcttgcc
     7081 gtttattaaa ccacggtttt gccttttcca gttgtgcagc cagtcttaat aacaatgctt
     7141 cctggccaaa cgctgccata aactgtactc cataaggtaa gccatctgga ccccagtgca
     7201 ggggtactga catggcggga ataccggtta catttgccaa ctgtgtgaac ggtacctgtg
     7261 caaggttctg aactgctaat ttatcaacaa caccgctggc tatggctagt tttccaagac
     7321 caaaaatatt tacaattctt gaaccaaata attcaattgg ttttggagca agtgaaccaa
     7381 tgggtgcagg aggttgggca gttgttggtg tgaggtatat atcatacttt gtaaagaatt
     7441 gccccattgc tcgtgccgct ttattccatt cgcgaagtgc cataacaaaa tctccggcag
     7501 tgtatgtcct gcctaaaagg cctaatgtcc aggtggttac ttctacatcg ctgcgttttg
     7561 gttttcgtcc taatttgatg ccaatggtcc taatttcagc agcaacctca ccaaagtata
     7621 gcataaggta gctttttgcc agtgccaggc cgtcaatatt aggtttatca atatcaacat
     7681 aatgacctaa tttttcgcac aattttgctg cttccagggc agcatttttg cattcaggat
     7741 gaacaggtga accaattggt gaatctgttg atagggctat tttaagttta ccaggtgctt
     7801 tctgtatttc tttgctatat ggcatttttg gtggttcaat gatatacgga gcaccggggt
     7861 cagagccctg caaaatatca agcatggcag cactgtcacg cactgagcgt gttaaaacat
     7921 gttccacagc agccccctgc caaatttcac cgtattccgg acctgtaggt acacgccctc
     7981 gtgacggttt aagtccaaat agaccgcagt aggcagaggg tatcctgatt gatccgccac
     8041 catcgccacc acctgcaaaa ggtaccatcc cgcttgcaac ggcagcagca gaaccaccac
     8101 ttgaaccgcc aggtgtcaga gcagtattcc agggatttcg cgttgggcca aaaatttcag
     8161 gttcagtata tgcaaccagt ccaaattccg gagtattggt tttgcctaaa atgacaagac
     8221 cggcattttt atagcgtttg gcaacctcac tgtca
//
LOCUS       DAOSZP010000016         8142 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_7514_length_8142_cov_3.720539, whole genome shotgun sequence.
ACCESSION   DAOSZP010000016 DAOSZP010000000
VERSION     DAOSZP010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 8142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8142
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7514_length_8142_cov_3.720539"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(24..1568)
                     /locus_tag="PK348_00770"
     CDS             complement(24..1568)
                     /locus_tag="PK348_00770"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HXK64776.1"
                     /translation="MKYNESVMLKPVYHNYTVFIISIASIIIIAYITALGFSYKFSRP
                     VEAIINALKKFDEGTTSIQIPVINQKDEIGKLAITTQHLLDMVNLEIKALTSTNMELL
                     RLHKLKDDFLANISHEIKIPLESITHLTNSIIHSYSTDETAAVTSSLEMISNSALRLS
                     YMIDDILDFTRLKNNDIILNKKVVDIAGVVNFVLSILQPVISSKSLSITIDINKKATH
                     VIADEQRLQQILLNIIGNAVKYSQKGTISIVTEKHNDTTLSIVVQDSAGGFPEQLIHH
                     IDDFGYDSKLQETPYGGLGLGLVITKKLMELHNGTIAIESIPDKGSTVTLYFPYDEAS
                     IDKELAATKSYIEYTIHPSGKDEHYLQSQFEKSQGFIYIVDDDPVNVKILYDMLTDAG
                     YYVEFSHEDSGLFSMLQQEKLPDIVLLDTVLPGTSAFQVCEKIRKRYSLYELPIIIIT
                     SKHRTQEILTAFRIGANDYLSKPFNKDELIARISNLIALKKSVEEHNEFVMIKHEIRL
                     AHEIHS"
     gene            complement(1625..2044)
                     /locus_tag="PK348_00775"
     CDS             complement(1625..2044)
                     /locus_tag="PK348_00775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK64777.1"
                     /translation="MKIRYKFFIAFIIIIAITTPVPIYILNRQIEQQHASIITEGTIR
                     AKQISHAILNMLLLNSGNIPQTSIDANEYISSFNPMFASGLICLQVILITPNPAYNGV
                     LLVDIPQGKTLSHIPPEHLQAIQKQPAVFFQLFREQN"
     gene            complement(2046..3122)
                     /locus_tag="PK348_00780"
     CDS             complement(2046..3122)
                     /locus_tag="PK348_00780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK64778.1"
                     /translation="MKLKLINFLYVFCVITVVLLNCNIFAQTYFKDNVTVGTQAGYAV
                     ITGYYSDSLNNGLGAGFFSFVPLTDLLMLNGVLVYNRFSLANSSNSNMQSFGITITPS
                     LYYALPFNITMYAGAGLSLQYYTLSAIKTDAYDSTTKTGFLMNAGIAKTLFERTLLIA
                     ECMYHITELSHKYFQTTVFSIKAGYQFALHKPEYYISQEAEKNAVREQTVKKLYTAAM
                     DYVQKKDHVHALETFKQVLSLKPDHKESKQYVAAISQAKEQYDTAGILIKQDKYFDAL
                     PLLAASSQYIAEASIDYKNVQDKLKPQIPELQQKAVEAFNNKDYDACITIMNSVLLID
                     PDNTVAKGYIVRSQRIKETIQKLQ"
     gene            complement(3166..3657)
                     /locus_tag="PK348_00785"
     CDS             complement(3166..3657)
                     /locus_tag="PK348_00785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK64779.1"
                     /translation="MASSSSTARQEINERIHIALTNTYSTLPQDNYVAFISIDSVDAI
                     INFPEHYGVPANWPIYSATGLLIAYNWNDLLDGTIIGELQNLGVCDSFWWSGSLQDGS
                     CDTDYTCNTWTSPTTLSSSSTLAVIPVLTGRSGAHNRTNYEWMYSNDRNCNDKLHLLG
                     LCW"
     gene            4785..4857
                     /locus_tag="PK348_00790"
     tRNA            4785..4857
                     /locus_tag="PK348_00790"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4818..4820,aa:Lys,seq:ttt)
     gene            4914..4986
                     /locus_tag="PK348_00795"
     tRNA            4914..4986
                     /locus_tag="PK348_00795"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4948..4950,aa:Glu,seq:ttc)
     gene            complement(5050..5514)
                     /locus_tag="PK348_00800"
     CDS             complement(5050..5514)
                     /locus_tag="PK348_00800"
                     /inference="COORDINATES: protein motif:HMM:NF013782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HXK64780.1"
                     /translation="MPRIQLNALQAYNFSYYYKINVNDINLWGHVGIPEIIAIIHIVR
                     RDVLRSLGFDEHDLGDNSALIIADVAINLIKELHLDDEIIIETAIGEIGNKSFRFFHK
                     VCHDNSIAAIIEMGAVVIDRVNRKSTFISEEFLNKIVSFSDTILQEPPHCKK"
     gene            complement(5654..6529)
                     /locus_tag="PK348_00805"
     CDS             complement(5654..6529)
                     /locus_tag="PK348_00805"
                     /inference="COORDINATES: protein motif:HMM:NF026048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HXK64781.1"
                     /translation="MNKEYLLNHLESMWNRGLIECHATGNTRTYNAVPWIPGMYEVQL
                     KFIDKEFAHLHTKYIKTVGHYFLTPKPQIMQVIPIEQHVQSHSSPMPYEQLSAIINNS
                     RSFAVNECICKKQTALLNRGCNKPREVCLSISESPNYFDNHPHAGRIITKDEALSILK
                     MAEDAALVHMTANIQEGHYFICNCCGCCCVQLIAARFGMPETVNAHFYALINPELCTQ
                     CGTCETRCQIQAIQNTDGLRSIIQQKCIGCGLCVSTCPNNAITLIHKDKSQDTVPPKD
                     QDDWYREKSKSRYAI"
     gene            7042..7629
                     /locus_tag="PK348_00810"
     CDS             7042..7629
                     /locus_tag="PK348_00810"
                     /inference="COORDINATES: protein motif:HMM:NF028235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HXK64782.1"
                     /translation="MLPKHNAKHDIVAISASLMHLKGFNNTGIQEILQRANIPKGSFY
                     HYFKNKEDLGLQVIDYYTEMITSLFKQYLQDTTVLPLERLDNLMTFYAQHFKKMKYKL
                     GCPLGNFALEMGDLSEEFREKLYGSIDTLISIIESCLQEAKTEGQIADTIDTRKAAEF
                     IFQSFEGALLYMKVSKSDRPLLVCKDAVKRYVQGK"
     gene            7708..>8142
                     /locus_tag="PK348_00815"
     CDS             7708..>8142
                     /locus_tag="PK348_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014811070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HXK64783.1"
                     /translation="MGQWKKTGCVLCAQNCGLEVEIENNTIVKVRGDKDNPRSKGYVC
                     RKGTNIMHHQHHDERLQYPLKRTDKGFVRISWDQAIEEIAAKLKEIIATYGPTSYAYV
                     GGGGQGCHFEAAFGVTLMRAIGSQYYYNALGQELTGLFWAYGR"
BASE COUNT         2566 a         1360 c         1525 g         2691 t
ORIGIN      
        1 ctgggtatat cctgtactac cacttaactg tgtatctcgt gtgcaaggcg tatttcatgc
       61 tttatcatga caaattcatt atgctcttct actgactttt tcagggcaat aagatttgaa
      121 atgcgtgcaa ttaattcatc tttgttgaag ggtttgctga gataatcatt tgcaccaatc
      181 ctgaaagctg tcagtatttc ctgagtgcgg tgctttgatg taattataat tattggtagc
      241 tcatataacg aataacgttt acgaattttt tcacatacct ggaatgcaga agttcctggc
      301 aaaactgtat ccagcaatac gatatcaggc aacttttcct gctgtaacat agaaaatagg
      361 ccagaatctt catgtgaaaa ttctacataa tagcctgcat ctgtcagcat atcatacagt
      421 attttgacat taaccgggtc atcatcaacg atataaataa atccctgtga tttttcaaat
      481 tggctttgca gataatgctc atctttacct gaaggatgta tggtatattc aatataagat
      541 ttagttgctg ctaattcttt atcaatactt gcttcatcat aaggaaaata caaagttact
      601 gttgaacctt tatcaggtat tgattctatt gcaattgtgc cattgtgcaa ttccatcagt
      661 ttttttgtaa taacaaggcc aagccccaat cctccatatg gggtttcttg aagtttacta
      721 tcgtatccaa aatcatcaat atgatgaatt aattgctccg gaaatcctcc tgcactatcc
      781 tgtactacaa tagaaagggt agtatcattg tgtttttctg taacaatgga tatggtacct
      841 ttttgtgaat atttaactgc attgcctata atattcaaca gtatctgttg caatctttgt
      901 tcatcagcaa tgacgtgggt tgctttttta ttaatgtcta ttgtaattga cagggatttg
      961 ctgctaataa ccggctgtaa tattgataat acaaaattaa ctacacctgc tatatcaaca
     1021 actttcttat tcagaataat atcattattt ttcaaccggg taaaatccag tatatcgtca
     1081 atcatatacg ataatcttaa ggcactatta gaaatcattt ccagagaact tgtaacagca
     1141 gcggtttcat cggtagagta gctatggata atagaatttg ttaaatgtgt tatgctttca
     1201 agaggtattt taatctcatg cgatatattg gccaggaaat catctttgag tttatgaagc
     1261 cgtaacaatt ccatattggt tgaagttaat gcctttattt ctaaattaac catatctaac
     1321 aggtgctgag ttgtaattgc cagttttcca atttcgtctt tctggtttat tacaggaatc
     1381 tgaatggatg ttgtaccttc atcaaatttc tttaaagcat taataatagc ttccacaggt
     1441 ctggaaaatt tatagctaaa tccaagtgca gtaatatatg caataatgat aatggaagca
     1501 atacttatga tgaatacggt ataattatgg tatacgggtt ttagcatcac tgattcattg
     1561 tatttcatat gcgttaaaca tgctattgca ctgtttgcca gggatgtttg ttcagtaaat
     1621 tcaatcagtt ttgttctctg aatagttgaa aaaacacagc cggttgtttt tggatagctt
     1681 gtaaatgttc tggtggtata tgtgacagtg ttttaccttg aggaatatca acaagcaata
     1741 caccattata tgccggattt ggtgtaatca gaataacctg aaggcaaatc agccctgagg
     1801 caaacatggg gttaaaactg cttatgtatt cattggcatc tatactggtc tgaggaatat
     1861 tgccactgtt taataacagc atgttaagta ttgcatgtga tatctgtttt gctcgtatag
     1921 tcccttcagt aataatgctg gcatgctgtt gttctatctg tctgttgagt atatatatag
     1981 gtacaggagt ggtaatggca ataatgataa taaatgcaat aaagaattta taccgtattt
     2041 tcatgttatt gcagcttctg tatggtttct tttattcgtt gtgaccgtac aatataacct
     2101 ttagcaaccg tattatcagg atctatcaaa agtaccgaat tcattattgt tatacatgcg
     2161 tcatagtctt tgttattgaa tgcttcaaca gctttttgct gaagttcggg aatctgaggt
     2221 tttagtttat cctgtacatt tttataatca atggaagcct ctgcaatgta ttgagatgaa
     2281 gcagctaaaa gcggaagtgc atcaaaatat ttatcctgct ttatgagtat ccctgcagta
     2341 tcatattgtt ctttggcctg agatattgca gcaacatact gcttggattc tttgtggtca
     2401 ggtttaagcg atagtacctg cttaaatgtt tccagcgcat gtacatggtc ttttttttgt
     2461 acatagtcca ttgcagcagt ataaagcttt ttaacggttt gttcccgtac tgcattcttt
     2521 tcagcttctt gagaaatata atactctggc ttatgtagtg caaactgata cccggcttta
     2581 attgaaaaaa cggtagtttg aaaatatttg tgcgatagct ccgttatatg atacatacat
     2641 tcagcaatga gtagcgtccg ttcaaataga gtcttggcta ttcctgcatt catcaaaaaa
     2701 ccagtttttg tggtggaatc atatgcatca gtttttatag cagaaagagt ataatactgt
     2761 aaagacaacc cggcaccagc atacatcgta atattaaagg gaagcgcata atacagtgat
     2821 ggagtaatag ttatgccaaa cgactgcatg ttagaattgc tgctgttagc aagtgaaaat
     2881 cgattgtaca caagcactcc gttaagcatt aataaatcag ttaaaggtac aaacgaaaaa
     2941 aatccagcac ccaaaccatt attgagacta tcactgtaat agcctgttat tacagcatac
     3001 cctgcctgtg tgccaactgt tacattatct ttaaaatatg tttgcgcaaa tatattgcaa
     3061 ttaagtaaga cgacagtaat gacacaaaat acatacaaaa aatttataag ctttaatttc
     3121 acctgtattt accagcatga ttgtaatata tattgaatac tactattacc agcacaaacc
     3181 taaaagatgt aatttgtcat tgcaatttcg gtcattacta tacatccatt cataattagt
     3241 tctgttatgt gccccacttc gtcctgtgag tacgggtata acagctagtg ttgaacttga
     3301 cgataaagtt gttggagatg tccatgtatt gcaggtatag tcagtatcgc aggaaccatc
     3361 ctgcagactg cccgaccacc aaaaactatc gcatacgcct aaattttgta gttcaccaat
     3421 aattgtacca tccagtagat cattccagtt atatgctatt aataaaccgg tagctgaata
     3481 aataggccag tttgcaggaa cgccataatg ttctggaaaa ttaattattg catcaacact
     3541 gtcaatactg atgaaagcaa cataattatc ctgaggcagt gtagaataag tatttgtgag
     3601 tgcaatatga atacgttcat taatttcctg gcgtgctgta ctgctggatg aagccatggc
     3661 accggtgtat gtgccagcac tgaaaagata tattctgcca atagtggtaa atgttgcagg
     3721 ataatagata tattgttatc aggatcgcat acacagacat ttacaaaata ttgcttccca
     3781 aataataagt ttgtgattat ttgttcagtt acaggctcac tggaccattt atcttcatca
     3841 agaagtccgg actgaagaat ttcttccaat gaaaaaagag tcaatgtgct actaacaata
     3901 acctggtatt gtaatgattc agggggagag ttgtcagttg ccaacgtcca ctgtactttt
     3961 gctgaatttt cagaaatatt agttactatt aagagcccat tattgccagg tataggtgca
     4021 gtactatcca aaaatgtatt agtatatgca ttttcctggt ctatgatagt attaaagttg
     4081 tcatctgtac atgatataca ataaataaca gcgataacca taaatattaa aatggttatg
     4141 cttctggtaa aatgtggaaa tgtatatttc ataactgtaa catggtaaaa atggctataa
     4201 aaagaaaatt accccaaacc atattaagtt aataaaattg ccaatatttt acaacttttt
     4261 tagcaattat ttttaaaaat tttttaaaat cgttagttat acatatcgta aatatataaa
     4321 caatatttat acatatgtac aataatacgg attgagtcaa actgttctta catgtatgta
     4381 ttaaaaatat ttatatagaa ataaaccctt cgtccaacct ttacggggtt gaatagtgat
     4441 gtaaaagatg tgctatttta tataaaattt cggcagtaat ttcattcaat tgtaattttt
     4501 atgaatactc ttcagagtat agcaatgatt tatatcactt tacctttcac ggcagagtta
     4561 atagagcttt tctatcctgg gaaacgtaat aaagcaatat aatgaattat aattgctatt
     4621 attaaacttt tttatgaaaa attttcaaaa tggtgtaatt ttttgaaaag caagaataat
     4681 tgaagtattt tttatttgac actaaaatac tattatttat ataaggttca ttcatttttt
     4741 tatttgcaat gtaatataaa atacgcaaaa gacgtttttt gcaggagtcg ctagctcagt
     4801 cggtagagca actgcctttt aagcagtggg tccggagttc gattctccgg cgactcaata
     4861 aaataatatt gacagtttca atgttatttt tttaagtagt ctcattatta catgtccccg
     4921 tcgtctaggg gtctaggaca ccgggttttc atcccggcaa caggggttcg aatcccctcg
     4981 gggatgcatt tacaaaggga gtgtctcaaa agacgctccc ttttttaata attattttac
     5041 aaatggttat tatttcttac aatgtggagg ttcctgcagt attgtatcag aaaaactaac
     5101 tattttattt aaaaattctt ctgaaataaa tgtggatttt cgattgactc tgtctatgac
     5161 cactgcaccc atttcaataa tagctgcaat ggaattatca tgacaaactt tatgaaaaaa
     5221 gcgaaagctt ttatttccaa tttcccctat tgcagtttca attattattt catcatcaag
     5281 gtgtaattct ttaatcagat ttatagcaac atcagctata ataagagcag aattgtcccc
     5341 taagtcatgt tcatcaaaac ccagtgaacg aagtacatct ctccgtacta tatgaatgat
     5401 tgcaataatt tctggtatac ctacatgtcc ccacaggtta atgtcgttaa cgttaatctt
     5461 atagtaatat gaaaaattat aagcctggag tgcattgagc tgtattcgtg gcatataaca
     5521 acaaccttac gttagttttt tgaattattt tgtaataaac aaattcttat atctcattgt
     5581 caatagtaat ttattcataa tagcgaaaca ttgttacgtt gtatgaataa atagtattgc
     5641 ttgatgaagt ttattatata gcgtagcgtg atttactttt ttccctgtac cagtcatcct
     5701 gatcctttgg tggcactgta tcctgagatt tgtctttatg aataagggta attgcattat
     5761 ttggacaagt agaaacacaa agcccgcatc caatgcattt ttgctgtata atagaacgca
     5821 gcccatcggt attctgtata gcctgaatct ggcatcgtgt ttcacaggtg ccgcattgtg
     5881 tacacaattc aggattaatg agtgcataaa agtgagcatt tactgtttca ggcatcccaa
     5941 atcgagctgc aatgagctga acgcagcaac agccacaaca attgcatata aaataatgcc
     6001 cttcctgtat attggctgtc atatggacaa gtgcagcatc ttctgccatt ttgagtattg
     6061 atagcgcctc atctttggtg ataattcttc ctgcatgagg atgattatca aaataattgg
     6121 gtgattctga gatagataag catacttcgc gtggtttatt gcatcctcta ttaagaagag
     6181 ccgtctgctt tttgcatata cattcattaa ctgcaaatga tctggagtta ttaattatgg
     6241 cagataactg ttcatacggc atgggtgatg agtgagattg aacatgctgt tcaatgggga
     6301 ttacctgcat aatctgtggt ttgggtgtta aaaagtaatg gccaacagtt tttatgtatt
     6361 ttgtatgaag atgtgcaaat tctttgtcta taaactttag ctgcacctca tacatgccag
     6421 gtatccatgg tactgcatta taggttctgg tgttgccagt ggcgtgacat tcaataagtc
     6481 ctcgattcca catactttct aaatggttca atagatattc tttattcatt ccagtacgag
     6541 cagctatctg atctatcgtt tccttttcca gagtaagttt gcaaaaaagc ttgccatcta
     6601 cgggtgtaga aaattttttg caagctcaag ttcaatgccg gtatcagtac aaagaaaacc
     6661 ggtagacatt gaattgcaat aaagagctaa ttaatggtaa ctatcgctta taaaaacatc
     6721 tgaagcctgg tattgaattt atacggtata tgcatgtaag tgttgagtta tttatataaa
     6781 taattaccgg gataaataaa gaacagaaag aataatttca aaaatgtatg aaacaaaaaa
     6841 atacagcaaa caccatgacc atgctaaggt catgcataaa ttgtagatag tgttgctttg
     6901 ggaatacatt ggtaaaaaga tgggaaatgc cataacgtga aaatttttgt aaaaatttta
     6961 caataggggt ggaaaaattc ttgacaaaga gacgaccggt ctactatatg aagaattgga
     7021 aaccattgaa gggttagggc tatgcttcca aaacacaatg caaaacatga tattgttgcc
     7081 ataagcgcaa gtcttatgca tttgaagggt tttaataata ccggaataca ggaaatcctg
     7141 caaagggcta atatacctaa aggctcattt tatcattatt tcaaaaataa agaggattta
     7201 ggacttcagg ttattgatta ttatactgaa atgattactt cactatttaa acagtatttg
     7261 caggatacca ccgttctgcc gcttgaaagg ctggataatc ttatgacatt ttacgcgcag
     7321 cattttaaaa agatgaaata taagttgggc tgtcccctgg gtaattttgc attggagatg
     7381 ggcgatctga gcgaagaatt cagagaaaaa ctgtatggct caatagatac gcttatttcg
     7441 atcattgaat catgtctaca ggaggctaaa acagagggtc agattgctga cactattgat
     7501 acgcgtaagg ctgctgaatt tatttttcag agttttgaag gggcgttgct ttacatgaaa
     7561 gtttctaaga gtgacaggcc cttactggta tgtaaagatg ccgttaagcg ctatgtacag
     7621 ggaaaatgag caatcgtcat ccagagcggt accaggtcat tctgaaggaa gcgaaggatc
     7681 tctaactttt atagtatgga gggtagtatg ggtcagtgga agaaaacagg ttgtgtgttg
     7741 tgcgcacaaa actgcgggct tgaggttgaa atagaaaata acaccattgt gaaagtgcgc
     7801 ggcgataaag ataatccccg cagcaaagga tatgtgtgca ggaaaggtac caatataatg
     7861 catcatcaac accatgatga acggttacag tatccattaa aacgaactga taaaggtttt
     7921 gtgcgtattt catgggatca ggcaattgaa gaaatagctg caaaactaaa agaaattatc
     7981 gccacctatg gacctacgtc atacgcatac gttggtggcg gtgggcaggg ctgtcatttt
     8041 gaggcagcct ttggtgttac tttaatgaga gctatcggct cccagtatta ctacaatgcg
     8101 ctgggtcagg aactcaccgg cttattctgg gcatacggca gg
//
LOCUS       DAOSZP010000017         7808 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_7962_length_7808_cov_3.149104, whole genome shotgun sequence.
ACCESSION   DAOSZP010000017 DAOSZP010000000
VERSION     DAOSZP010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 7808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7808
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7962_length_7808_cov_3.149104"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..221
                     /locus_tag="PK348_00820"
     CDS             <1..221
                     /locus_tag="PK348_00820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="protein TolA"
                     /protein_id="HXK64784.1"
                     /translation="EDKEAKKREEEEKLKKEKAEKEMQLKLEKERKAKMMAQKLEQQR
                     LEKEKAEREKAAKEQKIKEDRVKNIPTL"
     gene            283..1044
                     /locus_tag="PK348_00825"
     CDS             283..1044
                     /locus_tag="PK348_00825"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXK64785.1"
                     /translation="MHLHNVSLWRNSATILKNINLTIKYGEQWAVLGPNGSGKSFLMN
                     IIATLVFPSSGEVVIAGKKLGEVNVWDVRKHIGIVSDYLQYSYPAMTKTVEVVASGFY
                     SSLGLYQELSPTILQKAESILQTLGLEHYAQTPFGKLSYGEQKRVLIGRAIVYDPDIL
                     ILDEPCNGLDIRAREEFLHKLTDLVAIKKHIIYVTHHVDEIMPWINRVMLLADGQCMY
                     AGDRSVLEDEALLSRVMGYPLGIFHHNSRTYWYIK"
     gene            complement(1085..1396)
                     /gene="trxA"
                     /locus_tag="PK348_00830"
     CDS             complement(1085..1396)
                     /gene="trxA"
                     /locus_tag="PK348_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003498087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HXK64786.1"
                     /translation="MANIQEVTDATFDSAVLQSQGKVLVDFWAPWCGPCRLQTPILEK
                     LASNGINAKIVKCNTDVNPAIARKFGISAIPTLILFENGKEIDRMIGVQPEEVLKRRL
                     Q"
     gene            1960..2229
                     /locus_tag="PK348_00835"
     CDS             1960..2229
                     /locus_tag="PK348_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004102608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXK64787.1"
                     /translation="MKVTVEKINQEDLKKQGVFSWPIWQKEISDFDWYYDTTEQFYVL
                     EGEVEVETEDGQKVVFGPGDFVTFPKGVKCRWHVKKPIRKHYNFK"
     gene            2431..2961
                     /locus_tag="PK348_00840"
     CDS             2431..2961
                     /locus_tag="PK348_00840"
                     /inference="COORDINATES: protein motif:HMM:NF014543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK64788.1"
                     /translation="MKKISVCIAIAITGIVIVSCASTEKKVGCVEGKCNEGYGVYVWE
                     DGSKYAGSFKAGRFNGQGTFTWPDGRVYTGIYVDGKRQGYGVMSWPDGSVYKGQFAND
                     DITGNGTYKYADGKVYTGAMVKGKANGKGILEWPDGTRYEGEFIDNRMEGQGMLVYPD
                     GRTLKGRFVNGILVNE"
     gene            complement(3022..>3322)
                     /locus_tag="PK348_00845"
     CDS             complement(3022..>3322)
                     /locus_tag="PK348_00845"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015947239.1"
                     /note="Catalyzes the reversible hydration of unsaturated
                     fatty acyl-CoA to beta-hydroxyacyl-CoA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HXK64789.1"
                     /translation="LDLIITGREINAEEAYQFGLVNYLVKGDIKKEALALAQNIASFP
                     QNCLRSDRMCVYKGFNMPLPDAMEMEFARGTDVIRSGETLEGARKFAATKGKKLP"
     assembly_gap    3323..3332
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3333..3805)
                     /locus_tag="PK348_00850"
     CDS             complement(<3333..3805)
                     /locus_tag="PK348_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014185292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HXK64790.1"
                     /translation="MNIIREIVNDIIIITINRPEVRNAIDRPTAEELTEAFKNFDADD
                     NLKVAILTGADGNFCAGADLLAIAEDGHRKNKLNPNMKDDAPLGPTRMLLSKPVIAAI
                     SGYAVAGGLELACWCDIRMADSTAKFGVFCRRFGVPLIDGGTQRLPRLIGLSRAL"
     gene            complement(3819..4583)
                     /locus_tag="PK348_00855"
     CDS             complement(3819..4583)
                     /locus_tag="PK348_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014244941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HXK64791.1"
                     /translation="MANILTDDPGYTYHEKTPKIDAKEFQKVIDSRRSVRIYEKDPIP
                     EKIIRQCLHNALLAPNSSNLQPWEIYHIVDPAIRQQLNEACLGQIAAKSAATLFVAVA
                     RTNTWRQHAQEMVRLFESVGNVPKAAISYYKKLVPFVYNQGFLGTWGIVKRLLFFIRG
                     LKTPVIREPVSKCHIKIWAVKSTALACQNLMLSLRAYGYDSCPMEGYDSKRIKKILNL
                     PKDAVVVMVISAGKRAVGGVYGPRIRFDENRFLKKV"
     gene            4857..5162
                     /locus_tag="PK348_00860"
     CDS             4857..5162
                     /locus_tag="PK348_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011715238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
                     /protein_id="HXK64792.1"
                     /translation="MNSIPRKKIKYLKACAHGMKPVVLIGKNGLSQAVLNAIDEVLLS
                     HELIKIKFIDCKEERRELAQQIVEKTGSSLVTMIGNVLIIYRQHPEAEKRQYELAAM"
     gene            complement(5164..6123)
                     /locus_tag="PK348_00865"
     CDS             complement(5164..6123)
                     /locus_tag="PK348_00865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK64793.1"
                     /translation="MNIIIIYQKKNLPVLQKAFSAIIQSKKFSTAFVDSNESRKIQPP
                     DKNTIYYLDITGIKKAEIEKQVKKFNTNGHYFAIIDLKKSYTDIAWFFHNNACDYLTK
                     DMLVKPLSEKRMQSIMKLKGITPKTQHAHNYILAKDSWKGIVPGNEYTFCFLFIEILD
                     IGELKKHIGSEHLNQKIFSFRRYVEELLYQDDGRCWMWMDATGLFIFPFNGTSVPAIY
                     SAIRLIVNRLIISLEHTVFNQIIRYRIAMHIGNTVYQPRGKTGTIISDCINSLFHLGQ
                     KYTDEDGLFITENIYSFLNEKTKKLFIKVKPFEGRQIYTFKYK"
     gene            complement(6120..7043)
                     /locus_tag="PK348_00870"
     CDS             complement(6120..7043)
                     /locus_tag="PK348_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK64794.1"
                     /translation="MKIPYSILSITEKALTKSMDVHTMERIVKSIIPNYNLHKIMGFS
                     ESIPIPNKDAAAQIVRDFSESRFFFKLVHALMEAQEYGIMGRRYPIAHLHEIVGEIIN
                     LGFIYDKNNRIFIENSKTRKTPNWGVLEEDEEYIVTLLRLDIAANTRLVRKYESPLIT
                     QTYNNLRSIVTAAVEQRNGRVWNWEGDGCIAAFILGNRNMAAVCSAIEIFHELFLFNY
                     IESQLPEPVNIRVAIDTGYFTYKHSPEELKKTELVSKILELESRHTPLNSITITNSIY
                     ASLHAEIANKFTTTRSGNAVYYIYTIQWATP"
     gene            7183..>7808
                     /locus_tag="PK348_00875"
     CDS             7183..>7808
                     /locus_tag="PK348_00875"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HXK64795.1"
                     /translation="MGSYSLYDIPEADNGEVFQRFTEYFKNKDIQANASLSDKLKQFR
                     VQNKLSYEQLAAITGITSELLQKIEERHVYPSLKIIHQLTRSLQKAKAVAGSPVEWGY
                     TVIKKDEIIQSKNERPKERFHSGAHSHSAGTVNEAVAVKAYIITLEGNYTKKDFSIHN
                     GEEFIHVKKGAIKITLGANEVTLQQGDSIYFLSLIPHKIENIASHESI"
BASE COUNT         2410 a         1336 c         1640 g         2412 t
ORIGIN      
        1 aagaggataa ggaagccaaa aaacgtgagg aagaagaaaa attaaagaaa gaaaaagctg
       61 aaaaagaaat gcaactgaaa ctggaaaaag agcgtaaagc aaagatgatg gcacaaaaat
      121 tagaacaaca aaggctggag aaagaaaagg cggagcgtga aaaagcagcc aaagaacaga
      181 agataaaaga ggacagagta aaaaatatcc ctacgcttta aaacaatagt aaatatttag
      241 aagctccata gattatatgc taattcatat actatggagc ttttgcattt acataatgta
      301 tcactgtggc gaaattctgc taccatcctg aaaaatatta atcttaccat caaatatggg
      361 gaacagtggg ctgtgcttgg ccccaatggt tcgggaaaat cgtttttgat gaatattatt
      421 gctacgctgg tattcccttc tagtggggaa gttgttattg ctggaaaaaa attgggtgaa
      481 gtcaacgtgt gggatgttcg caaacatatt ggcattgtaa gcgattatct gcaatattcg
      541 tatccggcaa tgacaaaaac agttgaggtt gtagcatcag gtttttatag cagtttagga
      601 ttataccagg aactatcgcc cactatacta caaaaagctg aaagcattct gcaaacactg
      661 ggtcttgagc actatgcgca aacgccgttt ggaaaacttt cgtatggcga gcaaaagcgc
      721 gtgttgattg gcagggctat tgtgtatgat cctgatattc tcattttaga tgagccatgt
      781 aatgggcttg atatccgcgc acgtgaggag tttcttcata aactaacgga tcttgttgct
      841 ataaagaaac acattatcta tgttacgcat catgttgatg aaatcatgcc gtggattaat
      901 cgtgtgatgc ttcttgcaga tgggcagtgc atgtatgcag gtgatcgcag tgtgcttgag
      961 gatgaggcac tgttaagcag ggttatgggt taccctcttg ggattttcca tcataattca
     1021 cggacgtact ggtatataaa ataaacggcc ggttttcacc agccgtttta aagggtggag
     1081 tgggttattg gagcctgcgt ttgaggactt cttcaggctg tacgccaatc atacggtcaa
     1141 tttctttacc gttttcaaat aatatcagtg ttggtatggc gcttatgcca aatttacgtg
     1201 caatagctgg attaacgtca gtattgcatt ttacaatttt tgcattaatg ccattggatg
     1261 ctaatttttc caggatgggt gtctgtaatc tgcatgggcc gcaccatggt gcccagaaat
     1321 caacaagaac tttaccctgc gattgtaaca ctgcagaatc aaatgtagca tcagttactt
     1381 cttgaatatt tgccatcagt atgttcctcc gtaataaaat atctattaag aatataataa
     1441 aatacttaaa tatcggcaac aaaaatttta aaaaatccta ataaaaatgt ggcgatagtt
     1501 actggctaag gggtcagagc atgcgtgtac attatgcagg cattactata cttgatctgt
     1561 tttgtgcaca aaatgagttg cgatagttat cggtttagaa acaatgaaag ggtcagagca
     1621 tactataaag cgataattta tcgatgtatt gaatattttg gttgaaaatc acgtgctaca
     1681 ttgattatag tattatcaaa agactgccag tattattacc gaatactaca atgggaataa
     1741 agttctttat aatgtggcgc tactgtatgt gtagaggcta atattacaca aaaggttagt
     1801 acttacgttt acaatatgga ggtaatagtt gttcaagcat ttttcatgag aatgatgttg
     1861 ccgcctgagc tttgtaataa tgttacagat tttatcaaag aatgtgatgt gaatgatggt
     1921 aataaagtct attatccaac gaaaggaggg ataccataca tgaaagtgac tgttgaaaaa
     1981 ataaaccagg aagatttaaa gaaacaaggt gtattttcat ggccaatctg gcaaaaagaa
     2041 atttctgatt ttgactggta ttatgatact accgagcagt tttatgtact ggaaggcgag
     2101 gttgaggttg aaaccgagga tggtcaaaaa gttgtgtttg gccctggtga ttttgttacc
     2161 tttccaaagg gcgtgaaatg tcgctggcat gtaaaaaagc ctatacggaa gcattataat
     2221 tttaagtgat gaatgaatat taagtgttat tgagagcgct gaagggaaat tgtaatgact
     2281 ttctttaagt cactgtgagc aaagcgcatc ttctgagcaa ttcgacaggc gattactgag
     2341 ttcatcaaag ggcttagcaa ccttttgtgg aatttctact cagaaaagtg ttttgttctt
     2401 catactatgt tgaaaggagg ataattaata atgaaaaaaa tcagtgtttg tatagctatc
     2461 gccattactg gtattgttat tgtatcatgt gcttctacag aaaaaaaggt aggctgtgtt
     2521 gaagggaaat gcaatgaagg ctatggcgtt tatgtatggg aggatggttc taaatatgct
     2581 ggcagtttta aggctgggag attcaatggg caagggacgt ttacctggcc agatggtcgt
     2641 gtgtatacag gaatttatgt tgacggcaaa cgacagggat atggagtaat gtcctggcct
     2701 gatggcagtg tatacaaagg ccaatttgct aatgatgata ttaccggtaa tggtacgtac
     2761 aaatatgctg atggaaaagt atataccgga gcaatggtaa aagggaaagc aaatggcaaa
     2821 ggaattcttg aatggcccga tggtacacgc tatgaaggtg agtttattga taacagaatg
     2881 gaagggcagg gaatgctggt ataccctgat ggccgaacgt taaaagggcg ttttgtaaat
     2941 ggcatactgg ttaatgaata aggtaattat ttcactatac tgaatagtat tattgattat
     3001 ttatcaaaat gtatatacat actatggaag ttttttgcct ttagtagctg caaatttccg
     3061 tgcaccctcc agcgtttcac cactgcggat gacatcggtg cctcgggcaa attccatttc
     3121 cattgcatca ggtaatggca tattaaatcc tttgtatacg cacatcctgt cactgcgcaa
     3181 acaattttgt ggaaatgatg caatattttg tgctaatgct aacgcttctt tttttatatc
     3241 gcctttgaca aggtaattta ccaggccaaa ttgatacgct tcttcagcat taatttctct
     3301 gccggttatt ataagatcca gtnnnnnnnn nntccagtgc cctgcttaag cctataagcc
     3361 gtggtaaacg ttgtgtgccg ccatcaatta agggaactcc aaacctgcgg caaaataccc
     3421 caaacttagc tgtggaatcg gccatacgta tgtcacacca gcaggcaagt tcaagaccac
     3481 cggcaacagc atatccggat atggcagcaa ttaccggctt tgatagaagc attcgtgtgg
     3541 ggccaagcgg ggcgtcatcc ttcatgttgg gattaagctt atttttcctg tgaccatcct
     3601 cggcaatggc aagtaaatca gctccagcgc aaaaattgcc atctgcaccg gtaagtattg
     3661 ccaccttcag gttatcgtca gcgtcaaaat tcttgaatgc ttcagttaat tcttccgcag
     3721 tagggcggtc aatggcattt ctcacttcag gtctgttaat ggttatgatg attatatcat
     3781 ttactatctc ccgtattata ttcatacatg taaccctatt ataccttttt caaaaatctg
     3841 ttttcatcaa agcggatacg tggcccatag acaccgccaa ccgcacgctt acctgcagaa
     3901 atcaccatga caactacggc atctttggga agatttaaaa tcttttttat gcgttttgag
     3961 tcatagcctt ccatggggca tgagtcatat ccataagctc taagagaaag catcaaattt
     4021 tggcatgcca gtgcagtgga tttaacagcc catatcttga tatgacattt tgatactggt
     4081 tcacgtataa ctggtgtttt aagtccgcgt ataaagaata gcaaacgttt tactataccc
     4141 caggtgccta aaaatccctg gttgtataca aaaggaacaa gctttttata atatgagata
     4201 gcagcctttg gaacattgcc aactgattca aaaagcctta ccatttcctg tgcatgctgt
     4261 cgccaggtgt ttgtccgtgc tactgctaca aataatgtag cagcagattt ggcagcaatc
     4321 tgacccaagc aggcttcatt gagttgctgg cgtatagcag ggtcaacgat atgatagatt
     4381 tcccacggct gaaggttaga tgaattgggt gccagtaaag cgttgtgcaa acactgccgt
     4441 ataatttttt ctggtatagg atccttttca taaatacgta ctgaccttcg tgaatctatt
     4501 actttctgaa attctttggc atcaatcttt ggtgtctttt cgtgatatgt ataaccagga
     4561 tcatcagtta aaatgtttgc catttgtgct ccctccagaa aatgttttca tcagcttgtt
     4621 tatttcaatg tccaagtgca actacataaa aaaattattg tggtgcattt tgatacatgg
     4681 gattgcgccc aagcatgacg taaaaataaa ttaataaaaa attattgcaa taaaacacta
     4741 attaatttta tttgcaatta agctatcata gttatatatt aatgtcaatt ataaaaaaat
     4801 aaatgcggtg tgaatggatt gtgcaatatt aatattttta atttattgag gattgtatga
     4861 atagcattcc ccgcaaaaag attaaatatt taaaagcctg tgcccatggt atgaagccgg
     4921 ttgtgttaat aggcaaaaat gggctgtctc aggctgtact taatgccatt gacgaagtgt
     4981 tgctcagtca cgagcttatc aaaatcaaat ttattgactg caaggaagaa cgccgggaac
     5041 tggcacagca gatagttgaa aagacaggaa gcagtcttgt cacaatgatt ggtaacgtgt
     5101 taataatcta ccggcagcat cctgaagctg aaaagcgcca gtatgaactt gcagctatgt
     5161 aatttatttg tatttgaatg tgtaaatctg tctgccttca aatggtttta cctttatgaa
     5221 tagttttttt gtcttttcat taaggaaact atatatgttt tcggtgataa ataatccatc
     5281 ctcgtctgta tatttttgac ctaagtgaaa taatgaatta atacaatcgg atataatggt
     5341 accggttttg ccgcgcggct gatataccgt gttaccaata tgcatggcta tacgatagcg
     5401 tatgatttga ttgaacacgg tatgttccag tgaaataatt agccggttta ctataaggcg
     5461 aattgcagag taaattgcag gaacagatgt gccattaaag gggaaaatga acagcccggt
     5521 tgcatccatc cacatccagc aacgcccatc gtcctgataa agcagttcct caacatacct
     5581 gcgaaatgaa aaaatttttt gatttaaatg ttctgaacca atgtgttttt ttaattcgcc
     5641 aatatccagt atttcaataa acaaaaagca aaatgtatat tcattgccag gtacaattcc
     5701 cttccaggaa tcttttgcta gtatataatt atgcgcatgt tgtgtttttg gggtaattcc
     5761 ttttaatttc attatggatt gcatgcgttt ttcggacagc ggtttgacaa gcatatcttt
     5821 agtaagatag tcacaggcat tattatgaaa aaaccacgcg atatcagtat aacttttttt
     5881 aaggtcaatt attgcaaagt agtggccatt ggtattaaac tttttaacct gcttttcaat
     5941 ctcagctttt tttatgccgg tgatatccag gtagtaaatg gtgtttttat ccggtggctg
     6001 tatttttctg gattcattag agtcaacaaa ggcagttgaa aatttttttg attgtataat
     6061 tgcggaaaat gccttctgta aaacaggcag gttttttttc tggtatataa ttattatatt
     6121 catggagtag cccactgtat tgtataaata taatacactg cattgccact tctggtggtg
     6181 gtaaacttgt ttgcaatttc agcatgcagg gaagcataaa ttgaattggt gatagttatt
     6241 gagttaaggg gggtgtgtct ggattcaagc tccagtatct ttgacaccag ttctgttttt
     6301 ttcagttctt caggtgaatg tttgtaggtg aaatatcctg tatcaatggc tacccgtata
     6361 ttaacaggtt caggcagttg tgattcaata taattaaata aaaataactc atggaaaatc
     6421 tctatagctg aacatactgc tgccatattc ctgttgccca gaataaaagc ggctatgcat
     6481 ccatcacctt cccagttcca tacacgacca tttctttgtt caactgctgc agtaactatc
     6541 gaccgtaaat tattatatgt ttgggtgatt aatggcgatt catatttacg gacaagcctg
     6601 gtatttgctg caatgtcaag tcgaagcaat gtgacaatat attcttcatc ttcttcaagg
     6661 acaccccagt taggggtttt tcttgttttg gagttttcga taaaaatacg gttattttta
     6721 tcataaatga accctaagtt tataatttca ccaacgattt catgcagatg agctataggg
     6781 tagcgccgtc ccattatgcc atattcctgc gcttccatga gagcatgtac cagtttaaag
     6841 aaaaacctgg attcgctgaa atcccgtaca atctgcgcag ctgcatcttt gttggggata
     6901 gggatgcttt cggaaaagcc cattatctta tggaggttat aattgggtat gatacttttg
     6961 acaatacgct ccatggtatg gacatccatg gatttggtca gagctttttc cgttatcgat
     7021 aaaatggaat agggaatttt catggtatga tgatactata gtgcaacgca aatttgtcaa
     7081 aaaaaatttc ttgtaaaata attttttatc tgatagatga tatttttcag gatatacttt
     7141 atttgtcgca atgagtggaa tatatatttt aaggaaagta ccatgggctc gtattcatta
     7201 tatgatattc cggaagcaga caatggcgaa gtattccagc ggtttacaga atattttaaa
     7261 aataaagata tccaggccaa tgcatcactc agcgacaagt taaagcagtt cagagttcaa
     7321 aataaattaa gctatgagca gcttgctgcc ataaccggta ttaccagtga gctattgcaa
     7381 aagatagaag agcgtcatgt atatccttca ttaaaaataa tccatcaact tactcgttcg
     7441 ttgcagaagg caaaggcagt agcgggaagt cccgttgagt ggggatatac agttataaaa
     7501 aaagatgaaa tcatacaatc aaaaaatgaa aggccaaaag agcgcttcca ctcaggtgct
     7561 cattcacatt ctgctggaac cgtaaatgaa gctgttgcag taaaggccta tatcattacc
     7621 ttagagggca attatactaa gaaagatttt tccattcata atggtgaaga attcattcac
     7681 gtgaaaaaag gcgcaattaa gataacactg ggcgcaaatg aagttacctt gcagcagggt
     7741 gacagtatct attttctatc gcttatccca cataaaattg aaaatattgc ctcacatgaa
     7801 tcaattat
//
LOCUS       DAOSZP010000018         7754 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_8034_length_7754_cov_3.837252, whole genome shotgun sequence.
ACCESSION   DAOSZP010000018 DAOSZP010000000
VERSION     DAOSZP010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 7754)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7754)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7754
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8034_length_7754_cov_3.837252"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(142..879)
                     /gene="trpA"
                     /locus_tag="PK348_00880"
     CDS             complement(142..879)
                     /gene="trpA"
                     /locus_tag="PK348_00880"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF012511.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HXK64796.1"
                     /translation="MTYNGFYLVGNYPDPQTFLQAAIEGLRYFDFIEIGLPFSDPVAD
                     GPVLASASHKALEAGVTTESVLQSCSALHEYCIKHNRSKNIYIMTYANKVFYNDIDTT
                     FEKFALHGIKGIILADVPYIESQRFAKRAKAHHLDYIHFITPESTPEQIKNICSQATG
                     FLYTVSLRGTTGSTLVITDEMQHILSTAKHFAKVPVVLGFGIQGKEDIIQALHYADGF
                     IMGTALVKKLEEGLDAYKDFLKEIFWR"
     gene            complement(876..2051)
                     /gene="trpB"
                     /locus_tag="PK348_00885"
     CDS             complement(876..2051)
                     /gene="trpB"
                     /locus_tag="PK348_00885"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HXK64797.1"
                     /translation="MTYPDNNGYFGNYGGRFVPETLINALIELEDAYKHFLQDTSMQQ
                     ELNELLHHYAGRPTPLYFARRLSEQTGIVLYLKREDLLHTGAHKLNNTLGQALLTRFM
                     GKKRIIAETGAGQHGVATATVAALLGLECTVYMGAVDIERQMPNVKRMKLLGAEVVPV
                     TSGSKTLKDAINEALRDWVRNVRTTHYLLGSVSGPHPFPMIVRDFQKVIGNETKQQFM
                     TLTGKLPEFCVACVGGGSNAAGFFHAFINTSTRCIGVEAGGKGLYPGGHCASLTLGKP
                     GILHGALSYLLQNDDGQVMDVHSISAGLDYPSVGPEHSFWKDSNKVMYTTCSDKQALD
                     ACITLTRTEGIIPALESSHALGYVLENRNSFAGTSVIINLSGRGDKDLDTILKEVAL"
     gene            complement(2035..2664)
                     /locus_tag="PK348_00890"
     CDS             complement(2035..2664)
                     /locus_tag="PK348_00890"
                     /inference="COORDINATES: protein motif:HMM:NF012900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HXK64798.1"
                     /translation="MFVKFCGFTRPEDVEAACRLGVSACGFIFYTGSPRFCSFTTAKE
                     CIAICKEYGVTSAGVFVDNSPNAIASIACALGLDALQIYSEDAYVELSPNFTIFLGIR
                     VGKNFSMHVIPVIDHKDFYLFDTFDSSQYGGTGKQFDWENVASFPYINQTIISGGVNA
                     HNVKKLCATLNPFGIDVASGIEDAPGVKNISKMKQLIETIKECGYDISR"
     gene            complement(2654..3409)
                     /locus_tag="PK348_00895"
     CDS             complement(2654..3409)
                     /locus_tag="PK348_00895"
                     /inference="COORDINATES: protein motif:HMM:NF012444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol-phosphate synthase"
                     /protein_id="HXK64799.1"
                     /translation="MFSLAMMKRKKLQQRADVINLLQKFPKRNRPIFPLVCDSSINII
                     AEVKKASPSAGTIKDVDPATQATLYQKAGAKAVSVLADSYYFNGSFDDVYHVADAVRL
                     PVLCKEFICYPEQIDVAYSAGADMILLIASLLSSDELHYLFTYTTSKGLLPLIEVHTI
                     SELDSVLTLQPDYIMVNIRNLNTLELEYDIAIATLKSIPDNIQTICASGINNPETLQR
                     IKEQTGTTTFLVGTALMKANDPQKLLQELSHVC"
     gene            complement(3413..4417)
                     /gene="trpD"
                     /locus_tag="PK348_00900"
     CDS             complement(3413..4417)
                     /gene="trpD"
                     /locus_tag="PK348_00900"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HXK64800.1"
                     /translation="MNTEQLITQTIEGNTLSMEQSEYLFDSIFDGRISQIELAAVLAA
                     MKVRREKPEEISGAIKSMRKFAVSLPITESVFDTCGTGGDHSHSFNISSAVAIILNAM
                     GYPIAKHGNRSVTSKSGSADFYEALNIPVNLSGQDAVRYFNKHKFIFLFAPNYHPAMK
                     HAAPVRKQLGVRTIFNYLGPLTNPLQPKRQMIGVFHPSFLEVYAQAALPLGYERLILY
                     SSANGMDEVSPLHPTHVVEICNNKISTFTINPEKFLSNDEANAIPKDLSPQENATLFI
                     ETINNTTLTPLSKLLALNTALALFVLDDDSSIEDNYTVALDVIHSGSVAQTLTKLQEG
                     "
     gene            complement(4383..4976)
                     /locus_tag="PK348_00905"
     CDS             complement(4383..4976)
                     /locus_tag="PK348_00905"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HXK64801.1"
                     /translation="MFCIIDNYDSFTYNLVEYMRILGEDVDVYRNDANFESINFHNYT
                     GIILSPGPSSPAHSGITVTVIQNIHDIPILGVCLGMQAIGYVYGGRIVHAKKIMHGKT
                     DIIQHYGGNLFHNIPQQFTAARYHSLVVSETDFPNCLSIEARSSDGEIMALKHKTKFI
                     WGVQFHPESYASEYGLQILKNFTGGSYEYRTAYNANN"
     gene            complement(4976..6469)
                     /locus_tag="PK348_00910"
     CDS             complement(4976..6469)
                     /locus_tag="PK348_00910"
                     /inference="COORDINATES: protein motif:HMM:NF012640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HXK64802.1"
                     /translation="MYPTLQQCKSLCSTYNRIPIYKELNLSHPGLLLILKSFINNGDC
                     IFFESAKQNKKQSRFSFLGFNPAQTLLFSPPHIVISDSSQTKKILCSDPFDFIDTYIA
                     RYRSPSFKRFGSYNGGLSGYFAYDLVNYTGHLRQFIHQDTLHPLMVLHHIDDFICYDN
                     KYNTYYIATCIYTHDGSIESAYNQAIQCLHTYEDTIINTLSSTSLPYLPAYSESIDLD
                     FTSSPDEFMEKVSQAKTLIEDGEALQVVLSMRALINEPVDPYRFYLKLRQVNPSPYMF
                     YMKHGDLTVTGSSPEIHVKVQDTNATLRPIAGTIAQGKTKTQNKKNKEILLNNEKERA
                     EHLMLVDLARNDLSIIAKPGSVQVTQFMQPEDYSHVIHLVSNVTATLNDRISLSDVLR
                     HTFPAGTVTGAPKVRAIEIIDQLEPHPRGIYAGCVGYIGFNNTMDTCITIRTAVFSPQ
                     GSFLQAGAGIVYDSIPENEFNEIVHKLKALSVSLPFAKSKTMVKEIV"
     gene            7406..7729
                     /locus_tag="PK348_00915"
     CDS             7406..7729
                     /locus_tag="PK348_00915"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HXK64803.1"
                     /translation="MLTFEIEALQNIKERLLQKIPDHFVCMYAFGSRVRGDFTADSDF
                     DVLIVVKNKTPQIEDTIVGVIVEEELQHGISFTPLIKDSKSFELEQQYHTPFYSNIVN
                     EGIQL"
BASE COUNT         2282 a         1526 c         1550 g         2396 t
ORIGIN      
        1 tcatcgagcc tgtcgagatg aagccctttt tccccttgtg aaaagaatat tatggagttg
       61 ttgccgtctg gacaaaagaa agtccgaatg gaaagaatag ttacatagac taatatcagg
      121 aaaaaaaatt tataacagta actatcgcca aaaaatttcc tttaaaaaat ctttatacgc
      181 atcaagccct tcctctaact tttttaccag agctgttccc atgataaacc catcagcata
      241 gtgtagtgct tgtataatat cttctttgcc ctgaatgcca aatcccagaa caacaggaac
      301 ctttgcaaag tgttttgcag tggatagtat atgttgcatc tcatcggtta taacaagggt
      361 actgccggtg gttcctctca gtgagacagt atacagaaaa cctgttgcct gtgaacagat
      421 attttttatt tgttctggtg ttgattcagg tgtaatgaaa tggatgtaat ccaaatggtg
      481 tgcctttgca cgtttggcaa atcgttgtga ttctatatac ggcacatcag ccagtataat
      541 gcctttaatc ccatgtaagg caaatttttc aaaagtagta tcaatgtcat tataaaaaac
      601 tttatttgca tatgtcataa tatatatatt ttttgacctg ttatgtttaa tgcaatattc
      661 atgtaatgcg ctacagcttt gtaaaacaga ctcagttgtt acaccagctt caagtgcttt
      721 gtgtgatgct gatgcaagca ctggtccatc ggctacggga tcgctgaatg gtagccctat
      781 ctcaataaag tcaaaatagc gcaatccttc tatggctgcc tgtaaaaaag tttgcgggtc
      841 gggataattg ccaactaaat aaaatccatt atatgtcata gtgccacttc cttcaatata
      901 gtatctaagt ctttatcgcc acggcctgaa agattaatga taaccgatgt accggcaaaa
      961 ctatttctat tttcaagtac ataacccagt gcatggctgc tttcaagtgc ggggataatg
     1021 ccttcagtgc gcgtcagtgt aatgcacgca tccagtgcct gtttgtcgga acaggtggtg
     1081 tacataactt tattgctgtc tttccagaag ctgtgctcag gaccaacaga aggatagtca
     1141 agccctgcag aaatggaatg aacatccata acctgtccat catcattttg taacagatag
     1201 cttaatgcac catgcaatat accaggcttc cctaaagtaa gtgatgcaca gtgccctccg
     1261 ggatatagcc ctttgcctcc agcttcaaca ccaatacatc gtgtagaagt attaataaag
     1321 gcgtgaaaaa aaccagcggc attgctgcca ccgccaacac aggcaacaca aaactcaggt
     1381 aactttccgg tgagtgtcat aaattgctgc ttagtttcat tgccaatgac tttttgaaaa
     1441 tcacgtacaa tcattggaaa aggatgaggg ccacttaccg aacccagcag gtagtgtgtt
     1501 gtgcgtacgt tgcgcaccca gtcgcgtagc gcctcgttta ttgcgtcctt caaggttttg
     1561 cttcccgatg tcaccggaac aacttcagca cctaaaagct tcattctttt aacattgggc
     1621 atttgccttt caatatcaac tgcacccatg taaacagtgc actccagacc cagtagcgca
     1681 gcaacggttg cagtagccac gccatgctgt cctgcaccgg tttcagcaat gatgcgtttt
     1741 tttcccataa agcgcgtaag caaagcctgc cctaatgtgt tattgagctt atgagcaccg
     1801 gtatgcagta aatcctctcg tttaagatac agcactattc cagtttgttc agaaagtcgc
     1861 ctggcaaaat atagtggtgt tggtctgccc gcataatggt gcaataattc gtttaattcc
     1921 tgttgcatgg atgtatcctg caaaaaatgc ttgtacgcat cttctaattc tatgagagca
     1981 ttgatgagtg tttcaggaac aaaccttcca ccgtagtttc caaagtatcc attgttatcg
     2041 ggatatgtca taaccacact ccttaattgt ttctattagt tgtttcattt tggaaatatt
     2101 tttcacacca ggagcatcct ctatgccgct ggcaacatca atgccaaaag gatttaaagt
     2161 tgcacatagc tttttaacgt tatgtgcatt tactccaccg gaaataatgg tttggttaat
     2221 atatggaaat gatgcaacat tttcccagtc aaattgtttg ccagtgcctc catactgtga
     2281 tgaatcaaac gtatcaaaaa gataaaaatc tttatggtca ataaccggaa ttacgtgcat
     2341 ggaaaaattt ttaccaactc gtattccaag aaaaatagta aaattgggcg atagttccac
     2401 gtaagcgtct tcagaataaa tctgcagtgc gtccagccct aatgcacagg caattgatgc
     2461 aatagcatta ggactattgt ctacaaaaac gccagcactc gtcacaccat attctttaca
     2521 aatggcgata cactcttttg ctgtagtgaa gctacagaag cgcggcgatc ctgtataaaa
     2581 aataaaccca caggcgctaa cgcctaacct gcatgcagct tcaacatctt caggtctggt
     2641 aaatccgcaa aacttaacaa acatgtgata actcctgcag tagtttttgt ggatcgtttg
     2701 ctttcatgag ggcagttcca actaaaaagg tagttgttcc tgtttgctct tttattcgct
     2761 gtagtgtttc aggattattg ataccactgg cacaaatcgt ttggatatta tctggaatac
     2821 ttttcagtgt ggcaatggct atgtcatatt caagttcaag tgtgttcaaa ttccgtatat
     2881 taaccattat ataatcaggt tgtaaggtaa gaacgctatc aagttcagat attgtgtgca
     2941 cttcaataag tggcaatagt cctttactcg tagtatatgt aaaaagatag tgtaattcgt
     3001 cagaactgag caaacttgca ataagcagta tcatatcagc accagcgcta tatgcaacat
     3061 caatctgttc gggataacat ataaactctt tgcacaacac tggtaaccta acagcatcag
     3121 ccacatggta tacatcatca aagcttccgt taaaatagta actatcggct aaaacgctta
     3181 cagcttttgc accggccttc tgatacagtg ttgcctgtgt ggcagggtca acgtctttga
     3241 tggtgccagc tgatggtgaa gcctttttca cctctgcaat aatattaatg gagctatcgc
     3301 acactaaagg aaaaatgggg cgattgcgtt ttggaaattt ttgtagtaga ttaataacat
     3361 cagcacgctg ctgtaatttt ttacgtttca tcattgcaag tgaaaacatg gtttagcctt
     3421 cctgtagttt ggtaagtgtt tgtgcaacgc taccgctatg gatgacgtca agcgctaccg
     3481 tgtaattatc ttctatggat gaatcatcat ccagtacaaa aagcgccagt gctgtattca
     3541 gtgctaaaag tttgctaagt ggggtgagtg tagtattatt tatcgtttca ataaacagtg
     3601 ttgcattttc ctgcggtgat aaatctttag ggatagcatt ggcttcatcg ttacttaaaa
     3661 atttttctgg attaatggta aatgtgctga tcttgttatt acaaatttca acaacatggg
     3721 ttggatgtag tggtgatacc tcatccatgc catttgcaga agaataaagt atcaggcgct
     3781 catatcctaa aggcaatgca gcctgtgcat aaacttctaa aaatgaaggg tgaaaaacgc
     3841 caatcatttg acgttttggt tgtaatggat tggtaagcgg ccctaaataa ttgaatatag
     3901 tgcgcacacc caattgtttc cgtaccgggg ctgcatgttt catggcagga tggtaattgg
     3961 gtgcaaacaa aaatataaat ttatgtttgt taaaatagcg cactgcatcc tgcccagaaa
     4021 ggtttaccgg tatattcagg gcttcataaa aatcagccga gccacttttg ctggtaaccg
     4081 aacggttgcc atgttttgca attgggtagc ccatggcgtt aagaataatg gcaacagcgc
     4141 ttgatatatt aaatgaatgt gagtgatcgc caccggtgcc gcaggtgtca aagacgcttt
     4201 cagtgatggg aaggcttact gcaaatttcc gcattgattt aatggcaccg ctgatttcct
     4261 cgggtttttc cctgcgtact ttcattgcag caagcactgc cgctagttca atctgtgata
     4321 tcctgccatc aaagatagaa tcaaacagat attcggattg ttccatggat aatgtgttgc
     4381 cttcaattgt ttgcgttata agctgttctg tattcataag atcctcctgt aaaatttttt
     4441 aaaatctgta atccgtactc tgatgcgtac gattcggggt gaaactgtac accccatata
     4501 aattttgttt tatgttttaa agccatgatt tcaccatcgc ttgaacgggc ttcaatgcta
     4561 aggcagttgg ggaagtcagt ttcacttacc accagtgaat ggtaacgggc agcggtaaat
     4621 tgttgtggaa tgttgtgaaa taggtttccg ccataatgtt gaattatatc agttttaccg
     4681 tgcatgattt tttttgcatg aactattcta ccaccatata catacccaat tgcctgcatg
     4741 cccagacaca cacccagaat gggtatatca tgaatgtttt gaatgacggt tactgtaatg
     4801 cctgaatgcg ctggggatga agggcctggt gataaaatga taccggtata gttgtggaaa
     4861 ttaattgact caaaattcgc atcgttgcgg taaacatcca catcttctcc cagtatgcgc
     4921 atgtactcaa ccagattata ggtaaatgag tcatagttat caattataca gaacattaca
     4981 caatctcctt taccatagtt tttgattttg caaacggcag gcttaccgaa agcgctttaa
     5041 gtttatgtac tatttcatta aattcattct ccggaatact atcgtacaca atgccagcac
     5101 ccgcctgtaa aaatgaccct tgaggtgaaa agacagctgt cctgatggta atacaggtat
     5161 ccatggtatt gttaaatcca atgtagccca cacacccggc gtaaatcccg cggggatgcg
     5221 gttcaagctg gtcaataatt tcaatagcgc gcaccttggg ggctccggtt actgtgccag
     5281 caggaaaggt atggcgcagt acatcactta aactaatcct gtcattgagt gtagcagtaa
     5341 cgtttgaaac caggtgaata acgtgcgaat aatcttccgg ttgcataaac tgagttacct
     5401 gcacgcttcc cggctttgca atgatactta agtcattgcg tgctaaatca acaagcatca
     5461 ggtgttctgc gcgttctttt tcattgttaa gcaaaatctc tttgtttttt ttattctgag
     5521 tctttgtttt cccctgggcg atagttcctg caattggcct taatgtagca ttagtgtcct
     5581 gtactttaac gtgaatctca ggcgagcttc cggtaacagt aagatcgccg tgtttcatgt
     5641 aaaacatgta tggcgatggg ttgacctgcc tcagtttaag atagaaacga tagggatcaa
     5701 caggttcatt gatgagcgca cgcatgctca gcaccacctg caatgcctcg ccgtcttcaa
     5761 ttaaggtttt tgcctgtgac actttttcca tgaattcgtc tggtgaactg gtgaaatcta
     5821 agtcaattga ttcagagtat gctggcagat atggcagcga agttgaggat agtgtattga
     5881 taatggtgtc ttcataggta tgaaggcatt gtattgcctg gttataggct gattcaatgc
     5941 tgccatcatg tgtatagata catgtagcaa tgtagtatgt attgtattta ttgtcatagc
     6001 agatgaagtc atcaatatgg tgcagcacca ttaatggatg cagtgtgtcc tggtgtatga
     6061 attgtcgcag atgaccggtg tagttaacta agtcatacgc aaaataacct gataggcctc
     6121 cgttatatga accaaagcgt ttaaaagaag gtgagcgata gcgggcaata tatgtgtcaa
     6181 taaaatcgaa gggatcagaa cagagaattt tttttgtttg agaactatcg cttataacaa
     6241 tatggggagg gctaaataat agtgtttgtg ccggattaaa acctaaaaac gaaaatctac
     6301 tttgcttttt gttttgctta gcactttcaa agaagataca gtcgccattg ttgataaacg
     6361 atttcagtat cagcaaaagg cccggatgag aaagattaag ctccttatag atagggatgc
     6421 gattgtatgt actacacaaa cttttgcact gttgtaatgt tggatacatg gtattactcc
     6481 ttgctgtaaa ataaaaaagc cgtggattgg tttaacccac ggccatagtg ccataaaaaa
     6541 gccgtgggtt tgatgtccac ccacggcttt gccattttca tttatacaat aatagccctt
     6601 caggtggaca tcatgtttgt ccaccaccac cagctattgt taactgcaaa tgttaccagt
     6661 gttatcttca ttgctgtgtt acgtttaatg cctttaaaat tatatcgtta aatgacataa
     6721 aaactagaaa taaaaaatgt caacaatttt ttatttttta atatttacat catctgtctg
     6781 atgtttatct atgctctgtt ccacagggca tgattgcgaa agtcaggtac gaataccaag
     6841 ctttggaaat acataaatat tcaaagcaaa ccacaatgcc ataaggatca aaaaccatat
     6901 tgcttctttc atacgtgcac cttttacatt ttatccacag gatgaacctg tatcaaccat
     6961 aaaaccagca ccatacggtg tgtccacaaa atctatggta atgttggttc cagcatccat
     7021 aaaccttgca atatcgctgt catacattac ggggatttcg tttgcaatta ctactgtatc
     7081 ttttgtgttt tctgactcat ccagagccag tcccagacgg gggccgcctc atccaatacc
     7141 ggcaaaaaca agtcttaatc caggctgctt aaattcaaca tctttggcag cttcaataat
     7201 tttttgttta gctgaatctg taatagttat gttcatgata aaacctttac aataatttta
     7261 tataataata aaatactaac atttttggga ttttcaagta tttttttgtt tatatggcac
     7321 taattattgc ttttttccaa tattgttgac aggcaatcat gtattttgta tagttttaat
     7381 acgtgaagta aaaggtaaag catatatgtt aacatttgaa atagaagcct tacaaaatat
     7441 taaagaaagg ctattgcaaa aaattcctga tcactttgta tgcatgtatg catttggttc
     7501 acgtgtacgg ggtgatttta ctgctgattc tgattttgat gtgcttatag tagttaaaaa
     7561 caaaacacca caaattgaag atacaatcgt tggagtaatt gttgaagaag aattgcagca
     7621 tggtatatca tttactccac tcattaaaga tagcaagtca tttgaattag aacaacaata
     7681 tcacactcca ttctatagta atatagtaaa tgaaggtata caattatgac attgtcatcg
     7741 gaagataaaa agac
//
LOCUS       DAOSZP010000019         7698 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_8099_length_7698_cov_3.550177, whole genome shotgun sequence.
ACCESSION   DAOSZP010000019 DAOSZP010000000
VERSION     DAOSZP010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 7698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7698
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8099_length_7698_cov_3.550177"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..789
                     /locus_tag="PK348_00920"
     CDS             <1..789
                     /locus_tag="PK348_00920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK64804.1"
                     /translation="TVERELKVFESRKEKLATVESEIKALMELADMISLKADSLQDGM
                     LKIDQFNARIDALAKVYNDLENRIHELQEYETTITKNLESVNKADLIMKSIETRIKAF
                     QSTVEKSEKKIERLTGYLHSIEENTLILKTREQEIREVKEHFAELESLTAHIEERIKQ
                     VNAMFQKLEDMRDEISQTDSRLQLMFTETDKRMKQFADFLKAIESNTVIGKEIKGIPN
                     VNINERLIKTVRELSNKGWSSQEIANKLLIDENAVRLIINTSSL"
     gene            complement(796..1323)
                     /locus_tag="PK348_00925"
     CDS             complement(796..1323)
                     /locus_tag="PK348_00925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK64805.1"
                     /translation="MRKYILFIVVITMIACSTATVKEKEDPFAQDNKKFLEEKSGTEL
                     FRVLITPDNYQVVQLNYQGRITRNNDESGDKYILEEVAQYNQIDEAREGIVTVWLFPD
                     SGAIMKIRPTQLTFLVELDNLIVQDLQRWTFSFPEKTIEPTKFDVKYRIVLRKKLSDE
                     EIMQQIREKIKDKTQ"
     gene            1826..2467
                     /locus_tag="PK348_00930"
     CDS             1826..2467
                     /locus_tag="PK348_00930"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HXK64806.1"
                     /translation="MLDNLIQQLPVLLDSKSNPLLYLLLCISAILENLIPPVPGDVVT
                     AFGAFMVGTGKLHFFWVYLSTTLGSVIGFTLLFLVGKFFGKELLIKKNFTFFKKEAIL
                     KAEQWVQMYGIWLVLANRFLPGIRSVISLTAGFSNLSTYKVLLFSFISSSIWNLILIY
                     LGYSLGNNWLTVQGKVSHLLSQYSKVIVLLLLCLVIIYFFIKLKKYVAKKQLF"
     gene            2506..4905
                     /gene="lon"
                     /locus_tag="PK348_00935"
     CDS             2506..4905
                     /gene="lon"
                     /locus_tag="PK348_00935"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010692491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HXK64807.1"
                     /translation="MNFEEYFDNDTIQIENTILASDLSKIDTNLPSTIHIIPVRYRPI
                     FPGIITPLIIAKPRFMRSIDDALNTSRAIGLAVLKDDDVEDVEIDDIEMFGTAAKIIK
                     KINLPDESINVLINSICRFRITKIIDDKKSIIAKVEYIYDKANPKNLEIKALTRAILS
                     QLKILSESNPLFTEEMKLTMLNVDEPGRIADFVTSILNLEKQEYQNILETINVKKRLE
                     KVLVLLQKELEVVSVQRKIQNQINEKIYKQQRDYFLREQLKIIKQELGLDDDRSNEIQ
                     QMKSKIETLNLKGEPKEKIQDELNRLYYIESNTPEYIIARTYLDTVLSLPWNTYTEDC
                     LDITRAEKILKKSHYGLDDVKERILEFLAVRMLKPDSGGSILCLVGPPGVGKTSLGQA
                     IADSLKRTFFRVSLGGIRDEAEIKGHRRTYIGAMPGKIIQGLKICKSKNPVFMLDEID
                     KLYQSYQGDPAASLLEVLDYEQNHSFRDHYIDMPFDLSKVLFITTANTTDTIPDVLLD
                     RMEVIRIPGYIAEEKYKIAKHFLLPKQLKKHGLPKNSIELDRNAFFYIINGWAREAGV
                     RNLDRQLERLCRKKAKSIVKNDDSFPHLLDEESIRSLLGPEYYTGDTIPNKLKPGIAI
                     GLAWTSTGGSVLLVEALSTPAQGSSTLTLTGNIGDVMKESATIAYNYIKNYFSDDTQV
                     TDFFTKNTIHIHVPAGAIPKDGPSAGITMASALYSLVKHIPVTKRLAMTGELTLSGRV
                     LPVGGIKEKLIAAKREQCNTVIIPLENKKEMGDIPSHITRGLSLYFVAHVNEVFDIVF
                     K"
     gene            4955..6223
                     /locus_tag="PK348_00940"
     CDS             4955..6223
                     /locus_tag="PK348_00940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK64808.1"
                     /translation="MKKLLILLGILSFSVHVSAQIHGTAMGGISTVTSEGAIDTTRNP
                     ALLGTISAATTSFYIMGNTYYNEDANPEFHTPVMDISSIEQSNDYYYAFSLFAGYARP
                     SGRGILGYSLSSKENFYLRKKDTQKIYGSIPNPTPPPTSLSFEQTETTTTSEINPTLS
                     IAYGWKLSDNNYAGIQLTTTPFLSNKKTDNNNSLGTSYSYTNQEYGVIIQPSFGLLLS
                     GNDSQVGLRFIPSTIKCIKKKAEADFSTTDLSYSDKWDFQQSEGPQIVAGGYAKILPQ
                     TGIALEFGLLLPSSYTNTDINVTDNPLPAINHSSVTIHNDTIINAKAGIQYSFTETFE
                     CMAGAAFFHFINTAGSSKSYGKGKFNLVLITFGLNYSLSSTVILSTLLLINNSSFESY
                     YHMEDTISLDAKTKTNLWNITVGAGLSYRL"
     gene            complement(6245..>7698)
                     /locus_tag="PK348_00945"
     CDS             complement(6245..>7698)
                     /locus_tag="PK348_00945"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HXK64809.1"
                     /translation="EGVDIDINFGNPIAISPFLKSYLAKQKIKSKKLYLDPQELQSDV
                     LLRKIAIDIMYRYMRDIYAMTTLNHDHIFSYFLTKTKNRIKETDFKTKAYCAIESIKK
                     VNLATCHTSLQLKQGYLLTDDPHGRYNDFIDAAKSEGLISIKDGFIYKNKEKFSRLYE
                     FHTIRKDNIVEVLKNEIEPLEQVIKKLDRVFWTPMFCIRRKLVKSFYKRDREIFEKDY
                     KEFFKQGETKPKHIGMPLFKKRWFAHTGIVLVHGYMAAPEEMRQLAEFLYKKGYTVYC
                     ARLRGHGTSPEDLATRSWEEWYESVNRSYIVVKNSAKKVFICGFSTGAGLTLLHAANK
                     KDALQGAIAISAPYRLQNIAAGLAPVVDTWNKFLSFLKIKKIGRMDFIPNHPDNPDIN
                     YLRNPVSGIHQMEVLMNLVEKRLADISIPVLIMQGAGDKTVDPKGAFEMFMKIASEKK
                     EFTMVHSKSHGITRGEEGKVVARRVAAFIKDYA"
BASE COUNT         2503 a         1477 c         1319 g         2399 t
ORIGIN      
        1 actgttgagc gtgagctgaa ggtatttgaa tcacgaaaag aaaaacttgc tacagttgaa
       61 tctgaaatta aagctctcat ggagctggct gatatgattt cattaaaggc agattcatta
      121 caggacggta tgttaaaaat tgaccagttc aatgcccgta ttgatgctct tgcaaaagtg
      181 tataatgatc tggaaaacag aattcacgaa ttacaggaat atgaaactac cataaccaaa
      241 aatttagaat cggtcaataa agctgacctt attatgaaat caattgaaac aagaataaaa
      301 gcatttcagt caacagttga aaaatcagaa aagaagattg agcgtctcac aggatacctg
      361 catagtattg aagaaaatac acttattctt aaaacacgtg agcaggaaat tagagaagtt
      421 aaggagcatt ttgccgaact tgaaagcctc actgcacaca tagaagagcg catcaaacag
      481 gtaaatgcca tgttccagaa attagaagat atgcgtgatg aaatcagcca gaccgatagt
      541 cgcctacaac taatgtttac tgaaaccgat aagcgcatga aacagtttgc tgattttctt
      601 aaagctatag aaagtaacac agttattggc aaagagatta aaggcatccc aaacgtcaat
      661 atcaatgaga ggctgataaa aaccgttcga gagctctcca ataaaggatg gagttctcag
      721 gaaatagcca acaagctttt aattgatgag aatgcagtgc ggctgataat aaatacttct
      781 tctctgtaag gttttttatt gggttttatc ttttattttt tccctaattt gctgcattat
      841 ttcttcatca gaaagttttt ttctgagcac tatcctgtat tttacatcaa attttgttgg
      901 ttcaattgtt ttctcgggaa atgaaaaagt ccatcgctgc agatcctgaa caataaggtt
      961 atccagttca accagaaatg taagttgagt tggtcgtatc ttcattattg cacctgaatc
     1021 agggaataac caaactgtca ctataccctc gcgtgcttca tcaatttgat tatactgtgc
     1081 cacttcttca agtatgtact tatcgccact ttcatcatta ttgcgtgtga ttctaccctg
     1141 ataattcaac tgaactacct gatagttatc gggagttatt aataccctga aaagctccgt
     1201 accacttttt tcttcaagaa actttttatt atcctgtgca aatggatctt ccttttcttt
     1261 aacagttgct gtactgcatg ctatcatggt tattaccaca ataaatagta tatatttcct
     1321 cataaacatc aacatctcct ttaaaacaga attcagtgta aagctacgta catgaagaat
     1381 atcgttgttt gtaactattg attctttgct ttcaactcag aacaatatat taaaatcgta
     1441 tatcaatata acattgtcaa taatttaata caattggatt tgcacataat aaatatgacc
     1501 agctcaccaa ccacctatta ttgtttttaa cctacttcaa tgtaaaaata tatactgtat
     1561 aagcatactt gacaaaataa tgagaacatt atagtgtcgt ctttatgaaa gattttctat
     1621 ccacttgaac aataacatta taacaagtaa atcaattttt ttcaatacct gtttatatta
     1681 tttgcaacaa tcagtcacaa tgttacataa tataccataa tcaatgcact tagtatcaat
     1741 ataaaagttt caataataac aggtaaatta ttttttacag taagtacatc tataattcct
     1801 gaaatttacc aaagaatgta tcattatgtt agataacctt atacaacagc tgccagtgtt
     1861 attagatagt aaaagcaatc cgttactgta tctacttctg tgcatttctg ctatactgga
     1921 aaacctgatt ccgccagtac ccggtgatgt tgttactgcg tttggtgctt tcatggttgg
     1981 aacaggtaaa ttacattttt tctgggttta cctgtcaaca acgcttggtt cggttatagg
     2041 atttacactt ctttttctgg ttggtaagtt ttttggcaaa gagctactca tcaaaaaaaa
     2101 ctttactttc ttcaaaaaag aagctatatt aaaagctgag caatgggtac agatgtacgg
     2161 aatatggctg gttttagcca accggttttt acccggtatt cgcagtgtta tatctttgac
     2221 tgcaggcttt tcaaatttat ccacatataa agttctgttg ttttcattta tcagttcatc
     2281 tatatggaat cttattctta tatacttagg atattcattg gggaacaact ggcttacagt
     2341 acagggtaaa gttagccacc tgttatcaca atatagcaaa gtaatagtgc tgcttttatt
     2401 atgtcttgtg ataatctact ttttcattaa attaaaaaaa tatgtagcca aaaaacaatt
     2461 attctgatag tattaaaatt acttatggtt ctgtggagat aattaatgaa ttttgaagaa
     2521 tactttgata atgacaccat ccagatagaa aatacaatac ttgccagcga tctatcaaag
     2581 attgatacca atctaccttc taccatacac atcatacctg tacgatacag gcccatattt
     2641 ccaggtatca taactccgct tatcattgca aaaccacggt tcatgcgttc aattgatgat
     2701 gcactaaata cctcacgggc tattggttta gctgtattga aagatgatga tgttgaagac
     2761 gtggagatag atgacataga gatgtttggt accgcagcaa agataatcaa aaagattaat
     2821 cttcctgatg aaagcattaa tgtcctgata aatagtatct gtcggttcag aatcactaaa
     2881 attatcgatg ataaaaaaag tatcattgca aaagttgaat acatctatga caaagcaaat
     2941 ccaaaaaacc ttgaaatcaa ggcattaacc cgggctattt tgagccagct taaaattctt
     3001 tctgaaagca accctctatt tacagaagag atgaagctta ccatgctgaa tgttgatgag
     3061 cccggccgaa tagctgactt tgttacatca atattaaatt tagaaaaaca ggaataccag
     3121 aatattctgg agacaattaa tgtaaaaaag cgccttgaaa aggtactggt acttttgcaa
     3181 aaggaactgg aagtagtttc tgttcagcgt aaaatacaga accagataaa cgaaaaaata
     3241 tacaaacagc aacgggatta ttttttacgt gagcagctaa agataatcaa acaggaatta
     3301 gggcttgatg atgaccgcag caacgaaatt cagcagatga aatcaaaaat tgaaacgctg
     3361 aatctcaagg gtgagccaaa agaaaaaata caggatgagc ttaaccgtct ttattatatt
     3421 gaatcaaata cacccgaata tatcattgcc cgcacctatc tggatactgt tctatccctg
     3481 ccatggaata cgtatacaga agattgcctt gatattacac gggcggaaaa aatcttaaag
     3541 aaaagccatt acgggcttga tgatgtcaaa gaacgcatat tggagttttt agcagtcagg
     3601 atgctaaaac ctgattccgg aggctcaata ctatgtcttg ttggccctcc gggagttggg
     3661 aaaacatcac tgggacaggc tatagctgat tccttaaaac gtacattctt cagggtatcg
     3721 ttgggtggca tacgtgacga agccgaaatc aaaggtcatc gtcgtaccta tattggggca
     3781 atgcctggaa aaataatcca gggcttgaag atatgcaaat ctaaaaatcc tgtgttcatg
     3841 cttgatgaaa ttgataaact gtatcagagt tatcagggag atcctgcagc ttcgcttctg
     3901 gaagtccttg attatgaaca aaaccattca ttcagagacc attacattga tatgccgttt
     3961 gatctttcaa aagtactttt cattaccact gccaatacca ccgacaccat acccgatgtt
     4021 ttacttgacc gcatggaagt tatacgcatt cccggctata ttgctgagga aaaatacaaa
     4081 attgcaaaac attttctttt acccaaacag cttaaaaagc atggtttgcc aaaaaattcc
     4141 attgaactgg acaggaatgc tttcttctac attataaatg gctgggcacg tgaggcaggg
     4201 gttagaaatc tggatagaca gctggaacgc ctctgcagaa aaaaggcaaa atcaattgtt
     4261 aaaaatgatg actcattccc tcatcttctt gacgaagaat caatacgctc attacttgga
     4321 ccggagtatt ataccgggga caccataccc aataaactaa aacctggtat tgcaatagga
     4381 cttgcctgga catcgacagg tggttcagtt cttctggttg aggcactttc cacgccagca
     4441 cagggttctt ccactttgac gcttacaggt aatattggcg atgtgatgaa ggaatcagca
     4501 actatcgctt ataattatat taaaaattat tttagtgatg acacccaggt aactgacttt
     4561 ttcaccaaga atactatcca tattcatgtc cctgcaggag ccataccaaa agatggtcct
     4621 tcagctggaa taacaatggc atcagcgctt tattcacttg taaagcatat accggttaca
     4681 aaacgtttag ccatgaccgg tgagttgacg ctttcaggca gggtattacc tgtgggcgga
     4741 attaaagaaa agcttattgc agcaaaacgt gaacaatgca atacagtgat tatacctctg
     4801 gaaaataaga aagaaatggg tgatatacca tcgcacatta cccgggggtt gtcactatat
     4861 tttgttgccc atgttaacga agtgtttgat atagtattta agtaacttat tttactcgtt
     4921 actttgtgta atatacaaga agatggggcg gtctgtgaag aaattattga tattattagg
     4981 tatattaagt ttttctgtac atgtcagtgc ccagattcat ggtacggcaa tggggggtat
     5041 atccacagtc acttccgaag gagctattga tacaacacga aaccctgctt tgcttggaac
     5101 aatatcagca gcaacaacat cattctacat catggggaat acatattata acgaagatgc
     5161 aaaccctgaa tttcatacac ccgttatgga tatttcttca attgaacagt caaatgatta
     5221 ctattacgcc ttttcacttt ttgcagggta tgccagacca tcaggtagag gtatactggg
     5281 ctacagtctg tcctcaaagg aaaattttta tttacgaaag aaagatactc agaaaatata
     5341 tggcagcatt ccaaatccca cacctcctcc cacatcatta tcatttgaac aaacagaaac
     5401 aaccacaacc agtgaaataa accctacatt gagtattgct tatggctgga aactttcaga
     5461 caataactat gcaggtattc aactgacaac aacaccattt ctttcaaata aaaaaactga
     5521 taataataat tctttaggaa ccagttactc ctacacaaac caggaatatg gtgttatcat
     5581 tcagccttct tttggattgt tactatcagg taatgattcg caggtgggat tacgatttat
     5641 cccttccact atcaaatgta taaaaaagaa agcagaagct gacttcagca caactgatct
     5701 ttcatatagc gacaaatggg attttcagca gtctgagggg ccacagatag ttgcaggtgg
     5761 gtatgcaaaa atactgccac aaactggcat tgcgcttgaa tttggattat tgctcccttc
     5821 ttcctatacc aacacagata taaacgtaac ggataaccca ctaccagcaa taaaccattc
     5881 atcagtaacc atccataatg atacaattat taatgctaaa gctggtatac agtattcatt
     5941 tacagaaaca tttgaatgta tggcaggtgc tgcattcttc cattttatca ataccgcagg
     6001 aagttccaaa agttatggaa aaggcaaatt caatttagta ttaataacct ttggattaaa
     6061 ttactcgtta tcctctacgg ttatactttc aacattgctt ttaattaaca attcttcttt
     6121 tgagtcctat taccacatgg aagataccat atccctggat gcaaaaacaa aaactaattt
     6181 atggaatata acagttggtg cgggtctatc atatagattg taagtttctg catgtatgtc
     6241 atgctcacgc ataatcttta ataaatgcag ccactctccg tgccaccact ttgccttctt
     6301 cacctcgggt aatcccatgg ctttttgaat gcaccatggt aaattccttt ttttccgagg
     6361 caatcttcat aaacatttca aacgcaccct ttggatcaac cgttttatcc cctgcaccct
     6421 gcataatcaa caccggtatt gatatatcag ccagccgctt ttcaaccagg ttcattaata
     6481 cttccatctg atgaatacct gaaacaggat tcctgagata gttgatatca ggattatccg
     6541 gatgattggg gataaaatcc attctcccaa ttttttttat ttttaaaaat gaaagaaatt
     6601 tattccaggt atccactact ggtgcaagtc ctgcagcaat attctgtaat cggtaaggtg
     6661 cactgattgc tattgctccc tgtaacgcat cttttttatt ggcagcatgt aaaagtgtta
     6721 aaccagcacc cgttgaaaat ccacatataa aaactttttt cgcactgttt tttacaacta
     6781 tgtagcttct gtttaccgat tcataccatt cctcccagct tcgcgttgcc agatcctccg
     6841 gcgaagtgcc atgtccgcgc aatcgtgcac aatacacggt ataccctttt ttatacaaaa
     6901 attcagctaa ctgtcgcatc tcttcaggtg ctgccatata tccatgaaca agcacaatgc
     6961 cggtgtgagc aaaccatctt tttttaaata atggcatccc gatatgcttt ggtttagttt
     7021 caccctgttt gaagaattcc ttatagtctt tttcaaatat ctctcgatct cttttataaa
     7081 aacttttaac aagctttctt ctaatacaaa acattggggt ccagaaaact ctatcaagct
     7141 ttttaattac ctgctccaat ggctcaatct cgttttttaa aacttcaaca atgttatcct
     7201 ttcgtatggt gtgaaattca tacaatctgg aaaacttttc cttatttttg tatataaacc
     7261 catccttgat tgaaatcaat ccttcagatt ttgctgcatc tataaaatca ttgtatctgc
     7321 catgtgggtc atcggttaac agataccctt gcttcagctg tagtgaagtg tgacatgtag
     7381 ccagattcac tttttttata gattctatcg cacaatacgc tttcgtttta aaatcagttt
     7441 ctttgattct gttctttgtt tttgttaaaa aatatgaaaa tatatgatca tgattaaggg
     7501 ttgtcatagc ataaatatca cgcatatacc tgtacatgat atcaatagct atttttctaa
     7561 gcaacacatc agattgcaac tcctgaggat ccaggtacaa tttcttactt ttaatttttt
     7621 gttttgcaag atagcttttt aaaaatgggc ttattgcaat gggattacca aaattaatat
     7681 caatatctac tccttcaa
//
LOCUS       DAOSZP010000020         7621 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_8209_length_7621_cov_3.237246, whole genome shotgun sequence.
ACCESSION   DAOSZP010000020 DAOSZP010000000
VERSION     DAOSZP010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 7621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7621
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8209_length_7621_cov_3.237246"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1106)
                     /locus_tag="PK348_00950"
     CDS             complement(<1..1106)
                     /locus_tag="PK348_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012531256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXK64810.1"
                     /translation="MNFSLTQEQQIIQETAAKFAKQELEPVASTLDTTKDRNILKSNL
                     KKLAALGFNGIAVDPRYGGTGAGSVAFSLVMTELGKACAATAVTTSVTNMVAEVIQSV
                     GTEDQKQRYIPPLCNGTFYAGSFGLTEAEAGSDPAAMKTTATDGGDWWILNGKKLFIT
                     SAEYAGVFVVWAVTNKHAAKGRGISAFLVEPHFEGFIVGRDEHKMGQLGSSTNEIILD
                     NCKVPKENLLGTLNDGFRIAVRELGGGRIGIGSMALGIGLTAMDYATSYAKERIQFDK
                     PIIHNQAIQWMIADNYTRLEAARLLLLQAAYKKEKGLPFMKEASMAKLFATEVANKAC
                     YDAMQILGGYGYTREYPVERLYRDVRVTTIYEGT"
     gene            complement(1121..1981)
                     /locus_tag="PK348_00955"
     CDS             complement(1121..1981)
                     /locus_tag="PK348_00955"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006422263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HXK64811.1"
                     /translation="MKPDTINTIGVVGAGQMGSGIACVAAVSGFTVILYDLTEELALK
                     GVTYINGLLSKDVAKGKITQDEKQRIMERVRATGEFHALADADFVVEAATENQDIKLK
                     IFKDLDTICRPQVICATNTSSIPIGRIAAATKRPDKVIGMHFMNPVPVMKLVEIIRAL
                     PTSQETFDVTWELSLKLGKTPAEAHDYPGFISNRILMPMINEAIYCLYEGVGTRESID
                     TVMKLGMNHPMGPLALADLIGLDTCLAIMETLYDGFKDSKYRPCPLLRRYVESGWLGR
                     KTGKGFYDYT"
     gene            complement(2005..3174)
                     /locus_tag="PK348_00960"
     CDS             complement(2005..3174)
                     /locus_tag="PK348_00960"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HXK64812.1"
                     /translation="MKAVIVSTVRTALGNFNGSLSSIPATQLGAIVIEEAIKRAGISK
                     EDVNEVIMGQVLPCGYGQNPARQAAIKGHLPWEVECLTINKVCGSGLKAVMLAAQAIQ
                     CGDADVVVAGGMENMSMAPYYLEKARFGYRMGDGQLKDHMVHDGLWDVVNDFHMGISN
                     ELCCEKYNVSREEQDRYAALSYERTLHAIQAGKFKEEIVPVTIAQKQKEIIFDTDECP
                     KPTPYEILATMKPAFKKDGVTTAGNASIISDGAAALVVMSQKKAKELGCRIMATIGAQ
                     ASAGIDMKYVLMAPILAIPKCLKKEGISLQDVDLYEINEAFSGSTVGVLKELGLDSLK
                     VNVNGGSVALGHPIGASGARILVTLLHEMIRQNKRVGLASLCLGGGEAVALIVKR"
     gene            3429..3899
                     /locus_tag="PK348_00965"
     CDS             3429..3899
                     /locus_tag="PK348_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK64813.1"
                     /translation="MNIETTTCISHEHLELLTLHANNHSMSLRTFISCLISFAAHCDD
                     VQVQYFKQVKYRNRNSGNWKRFHLVLYNDEYEFFMDVKKLWKMSLARIIEYCLDNVLY
                     DFLKFLTKAEEDEDYYTDNYRYGGYAFETGTKENIFYITLYWGPHPEILQKAMT"
     gene            4162..4899
                     /locus_tag="PK348_00970"
     CDS             4162..4899
                     /locus_tag="PK348_00970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK64814.1"
                     /translation="MMAKKYKAVTCIVLVHIVFILISCTSVEFVKTGEEYPALNETDE
                     VQVLLTIDKNQYDEIGIADIGGVSLPVRIEKAKQIARSKGGNVIVPVTMCTEEAQKRM
                     RSGYIIQSFYILKTKTEAIAPKEAIESKQITPPVEEEFIPEKQAATFQQLLQHYPSLQ
                     GQPFTNTMVPLRFYKIPPSLLVFGLSDYKLCLMQFDTDNTVLVFIPKEKITVIKNSID
                     TRSTIAIDFTPLGVYKGKYPVLKMLSE"
     gene            4919..>7621
                     /locus_tag="PK348_00975"
     CDS             4919..>7621
                     /locus_tag="PK348_00975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK64815.1"
                     /translation="MVQKVSPQSSLCRFTLNDEEKLSDERVKEEIRKAFQELRAFPFV
                     VNSGIIDSLKKGADPSSLKDLFVAIRNDIFAAITDSPQAKEKIVSDTRLQERILNAII
                     TIHSAIAKGDFTSVINRRFVLIKERPAWPTLYYISPQSAVLSHVGQGPQWTEIPTIYL
                     GLNIFNVLALDKDQKFDKAFYWLLRVEERAIETGYSHIEVYPPHVSRALNELLDEVIR
                     IATISEVEFKEIPARKKIRAFTIRNRESFLKMLNARRDDGNFDFEKNLKAIRSLERLA
                     RRYKKGNDFNSLREIVRLLVAASGHDIHEIRNNANILLERIFAPKEFDAPLANTFVNC
                     AAGSIHTFEFSIPEEGNYFVRIFYNAGTNPYPLEQDIAYSDIDLAFDETKRVYSAHYT
                     FNELGHYDFLVYRKRKKRSQWVTYEGCSGRINVIPDVRGEIVLEIFPDIHGHTRIYWM
                     DEEHPGLVYNEHGEVIRLGRFSDITAHLEDLKERYHITALYLLGVQKRGTNREDWAPE
                     ATSPSPFSPVSLVEIEDFLGGENEFRELVSKAHAMDIKIIIDVVPHLNRRSTELPDEY
                     AVKCYDDSGNLVIRASTDGRYGSWNDGKLLNYRKLEVWEWLINSVVTLIDKYDIDGIR
                     FDSAHAVPIMMKKNNYPFIWGQYRSLESLVEGEIIVNDREDGHFITTGYFDSACRDQI
                     AIPFHHLLMCRIAQKLKEKNKTFFVHLAECYWGHERYLTRSGIIPYNSALFKICEGII
                     HGTTDVREVYHFYDNYLPYALPPGTELLGILGNHDERRALNTFGHRGLRAAIGLTIFM
                     NNIIMDYEGSAEGESWKVYLDNIYVNWNQFEYAAHRSLESFYRQWYRFHRNNKGKGYL
                     IWANNTMVAASIKFTEHTIWIGVFNFADSSQNVALQFDNPRLPIA"
BASE COUNT         2220 a         1659 c         1459 g         2283 t
ORIGIN      
        1 cttgtccctt cgtagatggt ggtgacgcgc acatcacggt atagacgctc aaccgggtac
       61 tctctggtat agccgtaacc tcccagtatc tgcatggcat cgtaacaggc tttgtttgca
      121 acctctgttg caaatagttt tgccattgag gcttctttca tgaacggcaa acctttttct
      181 tttttatagg cagcctgaag taacagcagg cgtgcagctt caagccgtgt atagttatcg
      241 gcaatcatcc actgtattgc ctgattgtgt ataataggtt tgtcaaattg tatgcgctct
      301 tttgcatagc tggttgcata atccatagca gtaagtccaa tccccaatgc cattgaacca
      361 atgccaattc gcccgccacc taactcacgc actgctatcc tgaatccatc attgagtgtt
      421 cctagtagat tttcctttgg aactttgcag ttatcaagga ttatctcgtt ggtagaggag
      481 cccagttgcc ccattttatg ttcatccctg ccaacaataa atccttcaaa atgcggttcc
      541 accaaaaatg cactgatacc cctgcctttg gctgcgtgtt tattggtaac agcccacacc
      601 acaaaaactc ctgcatactc cgcactggtt atgaatagct ttttcccatt gagtatccac
      661 cagtccccgc catctgttgc cgttgtcttc attgccgcgg ggtcagagcc tgcctctgcc
      721 tcagtcaggc caaagcttcc tgcataaaag gtaccattgc aaagtggtgg aatgtaccgc
      781 tgtttctggt cttccgtccc caccgattga ataacctcgg ccaccatatt ggttactgat
      841 gttgttacag cagtggctgc acaggctttg cccagttctg tcatcaccag tgaaaacgca
      901 acgctgccag cacccgtgcc cccataccgc gggtcaacag caatgccgtt aaaacctaac
      961 gctgccagtt ttttcaggtt ggattttaat atgtttctgt cttttgttgt atccagtgtg
     1021 gaagcaaccg gttcaagttc ctgttttgca aatttagcgg ccgtttcctg aattatttgc
     1081 tgttcctgtg ttaaactgaa attcattggc accacctctt ttatgtgtaa tcgtaaaacc
     1141 cttttccggt tttgcgcccc aaccagcctg attccacata gcgcctgagg agcggacatg
     1201 gcctgtattt tgaatcctta aaaccatcat acagggtttc cataatagct aaacatgtat
     1261 caagcccaat aaggtccgcc agtgccagtg gccccatagg gtggttcatc cctaatttca
     1321 taactgtatc tatggactca cgagttccta cgccttcgta caggcagtat atggcctcat
     1381 taatcattgg catgagtata cggttggaaa taaatcccgg ataatcatga gcctcagccg
     1441 gtgtcttccc taatttgagc gatagttccc aggttacatc aaatgtttcc tgcgatgtgg
     1501 gcagtgcccg tataatctca acaagcttca tgacaggcac tggattcata aaatgcatcc
     1561 caataacctt atcaggacgc tttgttgctg cggcaattcg acctatggga attgaagaag
     1621 tattggtggc acatattacc tgtggcctgc agatggtgtc taaatcctta aatattttta
     1681 actttatatc ctgattttcg gtggcagctt ccacaacaaa atcagcatct gcaagtgcat
     1741 ggaactcacc cgttgctctg accctctcca ttatgcgttg cttttcatcc tgcgttattt
     1801 tcccttttgc aacatctttg gaaagcaacc cgtttatata cgttacacct tttaaagcca
     1861 attcttctgt taaatcatag agaataacag tgaatccgct tactgctgca acacaggcaa
     1921 tccctgagcc catctgtcct gcacctacaa cacctattgt attaatggta tctggtttca
     1981 tacacattta tctcctaaaa tacactatcg tttcacaatt aatgcaactg cttcgccacc
     2041 acccaaacac agcgatgcca aacctacacg tttattctgg cgtatcattt catgaagcaa
     2101 tgttaccaga atacgggctc cacttgcacc aatgggatgc cccagtgcaa cgcttcctcc
     2161 attgacattg acctttaaac tatcaagacc cagttctttc agcacaccaa ctgttgatcc
     2221 actgaatgct tcgttgatct cgtaaaggtc tacatcctgc aatgatatcc cttctttttt
     2281 aaggcattta gggatggcaa gtattggcgc catcaggaca tatttcatat caatgccagc
     2341 tgaagcctgt gcaccaatcg ttgccataat gcggcacccc aactctttgg cctttttctg
     2401 tgacatcacc accagcgctg cagcaccgtc actgataatg gaagcattgc ccgcagtggt
     2461 aacaccatct tttttaaacg caggcttcat tgttgccagt atttcatacg gtgttggctt
     2521 tgggcattca tcggtatcaa aaataatttc cttttgtttt tgggcgatag ttaccggaac
     2581 tatctcctcc ttaaatttcc ctgcctggat agcatgtaga gttctttcat atgaaagcgc
     2641 agcatacctg tcctgctctt ccctgctcac attatacttt tcacagcaca attcgtttga
     2701 gatacccata tgaaaatcgt tgaccacatc ccataatcca tcatgaacca tatggtcttt
     2761 gagttgacca tcacccatgc ggtacccaaa ccgtgctttt tccagatagt agggggccat
     2821 actcatattt tccatgccac cagctaccac tacatcagca tcgccacact gtattgcctg
     2881 tgctgcaagc ataactgcct ttaaaccaga gccacatact ttatttattg ttaaacattc
     2941 cacttcccag ggaagatgtc cctttattgc agcctgccgt gccggatttt gcccataacc
     3001 acagggaagc acctgcccca taataacttc attgacatct tcttttgaaa ttcctgcgcg
     3061 cttaatggct tcttcaataa caatggctcc cagttgcgtt gcgggtatgg aagacaagga
     3121 accgttaaaa ttacccagag cagtccgtac agtactgaca ataacagctt tcattggcaa
     3181 cctccatttt tgtatatcaa taatacataa acaaaactat tcattatata gtcaaaatgt
     3241 caaaaatatt taatggtaaa tgttaaaatt ttcaaatgta ccacaaaatc tatatataca
     3301 tactgtattt tcattatatt cagctatatt tattcaattt ctgtgttgct taaaacaatt
     3361 ttgcttgcaa aatactttat attaacgtaa tcataatact ataaaatata aactttggaa
     3421 ggatagttat gaacattgaa acaaccacat gtatttccca tgaacatcta gaactactta
     3481 cattacatgc caacaatcat tcaatgtcac tgagaacgtt tatctcttgc ctcattagct
     3541 ttgctgcaca ctgtgatgat gttcaggtac aatatttcaa acaggtaaaa tacaggaata
     3601 gaaatagtgg caattggaag aggtttcact tagtactcta taacgatgag tatgaattct
     3661 ttatggatgt caaaaagtta tggaagatgt cacttgctcg gattattgag tattgtttag
     3721 acaatgtact gtatgatttt ttaaaatttt taacaaaagc tgaagaagat gaagattact
     3781 ataccgataa ctatcggtat ggaggttatg cttttgagac aggcactaag gaaaatatat
     3841 tttatataac attgtattgg ggaccacacc ccgaaatact ccaaaaagct atgacctgat
     3901 aacgcaaaaa cactattcac gtaatacatc attaagtttc taaagacaaa tagtttttct
     3961 catggataaa attatatata gggagggcat actatttctt tttttaattt ttaaattgac
     4021 taatttaaaa atagcacttc atatttaact agtagtagta acaatatttc attttgcatg
     4081 gtacattgtt gtactggatt actgttagtt gtatcgatag tataatgata ttagtaaaat
     4141 taacattttt aaggggtaat aatgatggca aaaaagtata aagcagttac ttgtattgtt
     4201 ttggtacata tagtgtttat attaatatca tgtacatctg ttgagtttgt taaaactggt
     4261 gaagaatatc ctgcactcaa tgaaactgat gaagttcagg ttttgcttac catagataaa
     4321 aaccaatatg atgagatagg catcgctgat attggcggag tatcattgcc cgtacgtatt
     4381 gaaaaagcca aacagattgc ccgcagcaaa ggtggtaatg tgattgtccc agtgacaatg
     4441 tgtactgaag aagcccagaa gagaatgcgc agcggttata ttattcagag cttttatata
     4501 ctcaaaacaa aaacggaagc tatcgccccc aaagaggcaa ttgaatcaaa acagataact
     4561 ccaccagttg aagaagaatt tatacccgaa aagcaagctg caacatttca acaactgtta
     4621 caacactatc cgtcattgca gggacagcca tttacaaata ctatggtgcc actgagattt
     4681 tacaaaattc ctccatcact tcttgtattt ggactgtcag actataaact ttgtttaatg
     4741 cagtttgaca ctgataatac tgtactggta tttattccca aagaaaaaat cactgttatt
     4801 aaaaactcta ttgatacgag gagtactata gctattgatt ttacaccttt aggtgtttac
     4861 aaaggaaaat atcccgtttt aaaaatgctg tcagaataat ataaaaaggc actgcatcat
     4921 ggtacagaaa gtttctcccc agagtagcct ttgcagattt actctcaatg atgaggaaaa
     4981 attatccgat gaaagggtaa aagaagaaat acgcaaagct tttcaagagc tcagagcatt
     5041 cccctttgtt gtaaattcag gtattatcga ttcattaaaa aaaggtgccg acccctcatc
     5101 gttaaaagat ttatttgtag ctatccgaaa tgatatattt gctgcaatta ccgatagtcc
     5161 ccaggcaaaa gaaaaaatag tatccgacac cagattgcag gagagaattt taaatgcaat
     5221 tatcacaatc cacagtgcta tagcaaaggg cgattttact tcagtaataa accgccgctt
     5281 tgtgcttatc aaggaacgac ctgcctggcc aacactatat tacatttcac ctcaaagtgc
     5341 cgtactatcc cacgttgggc agggtccaca gtggaccgaa attcctacaa tttatctggg
     5401 tcttaatata ttcaatgtgc ttgcccttga taaagatcag aaatttgaca aagcatttta
     5461 ctggttatta cgggttgaag agcgtgcgat agaaactgga tattcgcata ttgaagtgta
     5521 cccgcctcat gtatcccgtg ctctcaatga attgcttgat gaagttatac gcattgcaac
     5581 tatctcagaa gttgaattca aagaaatccc tgcccgtaaa aaaatacgtg cttttacaat
     5641 ccgtaaccgt gaaagctttt taaagatgct caatgcccgt cgtgatgatg gcaactttga
     5701 ttttgaaaaa aacttaaaag caatacgatc actggaaagg ctggcacgcc gttacaaaaa
     5761 aggcaacgac ttcaattcgt tgcgtgaaat tgttcgtctt ctggtggccg catcaggcca
     5821 tgatatccat gaaatacgaa ataacgccaa cattttactg gaacgcatat ttgcaccaaa
     5881 ggagtttgat gcaccgctgg ctaatacttt tgtcaactgt gccgcaggta gtattcatac
     5941 atttgaattt tccatcccgg aagaaggaaa ctactttgtg cgtatatttt acaatgcagg
     6001 cacaaaccct taccctctgg agcaggacat tgcatatagc gacattgatc tggcctttga
     6061 tgaaacaaaa agagtctata gtgcacacta tacattcaac gaattaggcc actatgattt
     6121 ccttgtctac agaaaacgaa aaaaacgttc tcagtgggta acatatgaag gttgcagtgg
     6181 taggattaac gtcatccctg acgtgcgcgg tgaaattgtt cttgaaatat ttcccgatat
     6241 tcacgggcat actcgtatct actggatgga tgaagaacat ccgggattgg tgtacaatga
     6301 gcatggagaa gtaattcgtt taggccgttt tagcgatatc actgctcatc tagaggattt
     6361 aaaagagcgc tatcacatca ctgctctgta ccttttgggt gtccagaagc gtggtaccaa
     6421 ccgtgaagac tgggcacctg aagctacatc accttcacca ttttcaccag tcagtttagt
     6481 tgaaattgaa gactttttag gcggtgaaaa cgaatttcgt gagcttgtat ccaaagctca
     6541 tgcaatggat ataaaaatta ttattgatgt ggtaccccac cttaatcgcc gtagcactga
     6601 gcttccggat gagtacgctg tcaaatgtta cgacgacagt ggcaatctgg ttattcgtgc
     6661 ttcaactgat ggtcgttacg gaagctggaa tgacggcaaa ctgttaaact atcgcaaact
     6721 ggaagtatgg gaatggctga tcaacagtgt tgttacatta attgataaat atgatattga
     6781 cggcatccgc tttgattcag ctcatgcggt ccccatcatg atgaaaaaga ataactatcc
     6841 gtttatatgg ggacagtatc gttcactgga atcgcttgtt gaaggtgaaa ttattgtcaa
     6901 tgatcgcgaa gatgggcatt ttattacaac aggttatttt gacagtgcct gcagggatca
     6961 gatagctatc cctttccatc atcttctgat gtgtcgcatt gcacaaaagc tgaaggaaaa
     7021 gaataaaaca ttttttgtgc atttagccga atgttactgg ggtcatgagc gctatcttac
     7081 ccgcagcgga attattccct ataattcggc cctttttaag atatgtgaag ggatcatcca
     7141 tggcaccact gatgtccgtg aagtatacca tttttacgac aactatctcc catacgcttt
     7201 accgccgggt actgagcttt taggcatact tgggaaccat gatgaacgac gcgcactcaa
     7261 tacgtttggg caccggggac tcagggctgc tattggactt actatattta tgaataacat
     7321 cattatggat tatgagggaa gtgccgaagg cgaaagctgg aaagtatatc ttgataacat
     7381 ctatgttaac tggaatcagt ttgagtatgc cgctcatcgc agcttggagt cattttaccg
     7441 gcaatggtac cgttttcatc gcaacaataa aggaaaggga tatttgatat gggccaataa
     7501 cactatggta gctgcatcca taaaatttac cgaacatact atatggattg gcgtttttaa
     7561 ttttgccgat agctctcaga atgtagcatt gcagtttgac aacccacggc tacccattgc
     7621 t
//
LOCUS       DAOSZP010000021         7544 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_8301_length_7544_cov_3.581520, whole genome shotgun sequence.
ACCESSION   DAOSZP010000021 DAOSZP010000000
VERSION     DAOSZP010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 7544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7544
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8301_length_7544_cov_3.581520"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            102..704
                     /locus_tag="PK348_00980"
     CDS             102..704
                     /locus_tag="PK348_00980"
                     /inference="COORDINATES: protein motif:HMM:NF013695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HXK64816.1"
                     /translation="MMDNLYRKALTLSYFTVGYNILEGVASVIAGAFAGSIALVGFGL
                     DSFVESLSGIIMIWRFRKHGIVSLEEEEEIEHRALKLVGYTFFILAAYILYESVTKLY
                     YVEIPDTSLVGIIIAVLSIIIMPVLYIKKVQVGKTIGSKSLLSDAKETLACTFLSIAL
                     LLGLGLNYLWGLWQADPIVGIIVALYLVKEGREIVTDEED"
     gene            796..2064
                     /locus_tag="PK348_00985"
     CDS             796..2064
                     /locus_tag="PK348_00985"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HXK64817.1"
                     /translation="MGCTVIIGTQWGDEGKAKMIDYYSQKADIIVRYQGGANAGHTVV
                     VNGKKHVFHLIPSGILHEGTICVIGNGVVLDPVQLIEECQLLEKEGIDVRKRLYISDA
                     AHLLLPFHKEMDAAMEEARSNKIGTTKRGIGPCYADKCLRVGIRVGDIFDEEYLKERL
                     STALAVKNTQFERCYNRKGFSFDEIYDLLMQFKAYAGNLAINTPYYLHSEAKKGKNIL
                     LEGAQGNALDIDHGTYPFVTSSNPSIGGAIAGSGINPFLINEVVGITKAYVTRVGEGP
                     FPTEEKGEAGEIMRQRGGEFGATTGRPRRCGWFDMELLKHTIRINGITTIALTKLDVL
                     SGFETIKVAVGYTINGKKIDYFPSAMQDKIVPVYEEHPGWKEDISQCTSFKELPRNAQ
                     NYVKYIQDSLQVPISIISVGPDRNNTFEMK"
     gene            2069..2506
                     /locus_tag="PK348_00990"
     CDS             2069..2506
                     /locus_tag="PK348_00990"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HXK64818.1"
                     /translation="MNKEQLIELLKEFFTRHEHMWPVSMAFLYGSWAHDMQRNDSDID
                     IAVMFKENNPNEIFDYINAISLSLSDHLHRDVNVLYIDDDISKPMLHYNAIVKGEVLY
                     FDDFTHYVDVLLKALEQMEDFSMFGLSWQKQIAEKNLKDLTHA"
     gene            2499..2936
                     /locus_tag="PK348_00995"
     CDS             2499..2936
                     /locus_tag="PK348_00995"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HXK64819.1"
                     /translation="MPEFSYAKGRILESIAFVTCEMMEFEQEYADKKYSEYSSNTKLQ
                     KIIDRTIENILTAIIEMSGAILAENNIKTDSYAEVLRKIGSFFEFSDKECEILASFAI
                     QRNRLAHRYLNFRWQVVTFYNNNKDIITRLVQKMYEYESKKHE"
     gene            complement(2989..4110)
                     /locus_tag="PK348_01000"
     CDS             complement(2989..4110)
                     /locus_tag="PK348_01000"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HXK64820.1"
                     /translation="MPYTFELGPIRPPSEAYSILIRVTRNCPWNKCAFCHTYKGQQFS
                     RRPIQDVIADIDAIYTLAQRILDVASHGITMHTLQKAKGNDDTPLYYYEQVAFWLQYG
                     MKSVFLQDANSLVLSPNDLCTILNHIKERFPTVERITSYARASTISKKSLDDLKAIRQ
                     AGLTRLHIGMESGSDTVLALVNKGTTQQQLIDAGQKAVEANFDVSYYYMPGLGGKEYM
                     EENALQSALVINNVNPTFVRLRSTVPIPGTPLYEMMEQGTWTPLSEVEKVQEIRLFIE
                     HCNGISSTLTSDHMMNLLEDVEGTFPHDKATMLATIDEFLSMQQEDQEKFIVGRRVGI
                     YRYLKDYYPNFKVDQLYYSIKHQYGSIDKAVMDILKSFI"
     gene            complement(4323..7271)
                     /locus_tag="PK348_01005"
     CDS             complement(4323..7271)
                     /locus_tag="PK348_01005"
                     /inference="COORDINATES: protein motif:HMM:NF037510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease"
                     /protein_id="HXK64821.1"
                     /translation="MINISEKEMESSIEADLINAGYFKRNPSHYDKALCLDPEMLINF
                     IIATQKDKWEAYKKQLANKAQEALLTKIKDEIEKRGTLNLLRKPFASYGVYFDFAYFK
                     PASGLNESYRKKYQNNMFSVIRQVKYSQHNENSLDMVLFLNGIPLVTMELKDRMTGSG
                     YNVESAIRQYQNDRDPQEPLFRYGRCLVHFALDEELVYMTTHLKGQKTLFLPFNKGNN
                     GEAGNPFTGGFSTEYLWKEVLLKDSFLELLQHYIQETDVLDDDGKPTGEKKIIFPRYH
                     QMDCVRNIIKHAYENGTGQHYLIQHSAGSGKSNTISWLAHQLSNLHDKNDVNIFDSII
                     VVTDRRILDSQLRANVRAFEQNPGVVAGIEKGSKQLRQELEAGTKIIVTTLQKFPYIE
                     EEIKTLPGKRFAIIIDEAHSSTSGEMSRSLKKVLNLNSEDDPDEEDKTYEDEIIEQMK
                     SRGKQPNISYFAFTATPKPKTLELFGVPMPDGSYKPFHIYTMKQAIQEGFIKDVLPNY
                     TTYATYFKLVKKIIDDPKYDKVKASRLLKTYVELNEHTIDKKTAIIINHFMENCQHEI
                     NGLAKAMVVCSSRAQAVKYKLAFDKYIKENQLPFKALVAFTGVIESYNIGDSSQNEYS
                     EAKMNGFPEDQTASYFKESQYRFLIVANKFQTGFDQPLLYAMYINKKLQGVNAVQTIS
                     RLNRVYPGKKDPITIDFVNDSNTILQAFQNYYEDVFLEEGTDPDKLYDFKLTLESFKY
                     YTNQDVEDFVKIFFSKKFKQEDLVPIIHRVVKNYNEENDPNKKEKFRTSLKSYLRVYA
                     FLSQLISFTDPALEKLYVFGKILFRKLPYDKEKLPKEVTQQVDLESIKIQYLSKGIKV
                     KNGEGGKLKPGDENRNGIKYEFIESLSMIVKEINEKYGTEFTDEDKVVADTILSRLKS
                     DRYIEEIVKNNPKENVWYAFEKKFNEELQKLLEDNFNFYKKLNHNSDIKEEMMKKMFN
                     SLYENMRKAG"
BASE COUNT         2217 a         1267 c         1511 g         2549 t
ORIGIN      
        1 ggtccatgca agaaaagcaa ttagcataac ctgattattc tatagattta caataatttg
       61 aatagggctg taaaatattt tgacttttta taggaggaaa catgatggat aacctttacc
      121 gaaaagcatt aacattatca tattttacag ttgggtataa tatattagaa ggtgtagcct
      181 ctgttattgc gggtgctttt gccggcagta ttgctctggt tggttttggg cttgatagct
      241 ttgttgagtc actatcgggc ataatcatga tatggcgttt tagaaaacat ggtattgttt
      301 cactggaaga ggaagaggaa attgaacaca gagcgctgaa actcgtgggt tatacttttt
      361 tcattttagc agcgtatata ctctatgagt cggtaacaaa attatattat gtagaaattc
      421 ccgatacaag cctggttggg attattattg ccgtgctatc aattattatt atgccggttc
      481 tctacattaa aaaggtgcag gtagggaaga caataggcag taaaagtttg ctttcagatg
      541 caaaagaaac gttggcctgc acatttcttt caattgcgct actgttaggt ctgggactta
      601 attacctgtg gggcctgtgg caggctgacc ccattgttgg aattattgta gcactgtatt
      661 tagtaaaaga gggcagggag atagttaccg atgaagagga ttaatagttg aaaaaagctt
      721 gttattttta tatattcatg tagcgtggca gggttggcgt tacaattata aaataaaatc
      781 agaaaggaat ttgatatggg ttgtacagtc attattggca ctcaatgggg tgatgaaggc
      841 aaagctaaga tgattgatta ctattctcag aaggcggata tcatcgtacg gtaccagggt
      901 ggtgccaatg ctgggcatac agtggtggtt aatgggaaaa aacatgtttt tcatcttatt
      961 ccatctggta ttttgcatga aggtacaata tgtgtgatag gcaatggtgt ggttctggat
     1021 cccgtgcaac ttattgaaga atgccagcta cttgaaaaag aaggcattga tgtccgcaaa
     1081 cgattatata tctctgatgc agctcatctt cttttgccat ttcataaaga gatggatgct
     1141 gcaatggagg aagcacggtc aaataaaatt ggtactacaa agcgtggtat tgggccctgt
     1201 tatgctgaca aatgtctgcg ggtgggtatc agagttggtg atatctttga tgaagagtac
     1261 ctcaaggaga ggctttcaac tgcgcttgct gtgaaaaata ctcagtttga acgctgctac
     1321 aacaggaaag gtttttcatt tgatgaaata tatgaccttc tcatgcaatt caaggcgtat
     1381 gctggcaatc tggctattaa cactccttat tatttgcatt ctgaagccaa aaaagggaaa
     1441 aatattttac tggaaggtgc acagggaaat gcccttgata ttgatcatgg cacctatccg
     1501 tttgttacct catcaaatcc cagtataggt ggggcgatag caggttcagg tataaacccc
     1561 ttcttgataa atgaagtagt tggcatcacc aaggcgtatg ttacccgggt tggcgaaggc
     1621 ccattcccaa ccgaagaaaa aggtgaagct ggtgagatca tgcgccagcg tggtggtgaa
     1681 tttggcgcta caaccgggcg gccacgtcgt tgcggctggt ttgatatgga gcttttaaag
     1741 cataccattc gcataaatgg aataacaacc atagcattga ctaaattaga tgtattatca
     1801 ggttttgaaa caatcaaagt agctgttgga tatacgatta atgggaaaaa gattgattat
     1861 ttcccgtcag ctatgcagga taaaattgtg cctgtttatg aagaacatcc tggatggaaa
     1921 gaagatatat cacagtgtac atcatttaaa gagctgccca gaaatgcgca gaattatgta
     1981 aaatatatcc aggatagttt gcaggtgcct atctccatta tttccgttgg gcctgaccgt
     2041 aacaatacat ttgaaatgaa gtagttttat gaacaaagag cagttgatag aattattaaa
     2101 agaatttttt accaggcatg aacacatgtg gccggtatca atggcttttt tgtacggttc
     2161 atgggcacat gatatgcaga gaaatgattc tgacatagat attgcagtta tgtttaaaga
     2221 gaataacccc aatgaaattt ttgactatat taatgcaatt tcattatcac taagtgatca
     2281 tttacatcga gatgttaacg ttttatatat agatgatgac atttcaaagc ccatgctgca
     2341 ctataatgct attgttaagg gagaagtttt gtattttgat gatttcactc actatgttga
     2401 tgtattgctt aaagctctgg aacagatgga agattttagc atgtttggat tatcctggca
     2461 aaagcaaatt gcagaaaaaa acttaaagga tttaactcat gcctgagttc agttatgcaa
     2521 aaggaagaat actggaatca atagcatttg ttacatgtga aatgatggaa tttgagcaag
     2581 aatatgctga taaaaagtac agtgaatatt caagtaatac taaattacaa aagattattg
     2641 atagaacaat agaaaatatt ttaacagcta ttattgaaat gagtggtgca attttagcag
     2701 aaaacaatat aaaaacagac agttatgcag aggtattgcg aaaaattggt tcgttttttg
     2761 aattttcaga taaagaatgt gagatattag catcatttgc aattcagcga aatagattgg
     2821 cacatagata ccttaatttc aggtggcagg ttgttacatt ttataataac aacaaagata
     2881 ttataacccg attagtacaa aagatgtatg aatatgagtc taaaaaacat gaataagtaa
     2941 agtgttttag atcttgtagt gagttattta taaagtatcc tatctattct atataaaact
     3001 ctttaaaata tccattactg ctttgtcaat gctgccgtat tgatgtttga tagaataata
     3061 cagctgatcc actttgaagt ttggataata atcctttaaa tatcgatata tgcccactct
     3121 acgcccaaca ataaattttt cctgatcttc ctgttgcatt gaaagaaatt catctatagt
     3181 tgcaagcatg gttgctttat cgtgaggaaa tgttccttct acatcttcaa gtaaattcat
     3241 catgtggtca cttgtcagtg tgctgctgat gccgttacaa tgttctataa agagtcgtat
     3301 ttcctgtact ttttcaactt cggacaatgg tgtccatgtt ccttgttcca tcatttcata
     3361 cagtggtgtt cccgggatag gcacggtgct gcgaagtcta acaaaggttg gatttacatt
     3421 atttataaca agggcacttt gaagggcgtt ttcttccata tactctttgc cgcccaatcc
     3481 aggcatgtaa tagtatgata catcaaagtt agcttcaaca gccttctgcc ctgcatcaat
     3541 gagttgctgt tgtgttgttc ctttgttgac tagtgccagc acggtgtcag agccagattc
     3601 catgcctata tgaagacgcg tgagccctgc ctggcgtatt gcctttaaat catccagtga
     3661 ctttttgctg atggtactgg ccctggcata cgatgtgata cgctcaacgg ttgggaaacg
     3721 ctctttaatg tgattaagaa tagtacataa atcgttgggc gatagtacca gcgaattagc
     3781 atcctgtaaa aaaactgact tcatgccata ctgcagccag aaggcaactt gctcgtaata
     3841 atacagtggc gtatcatcat tgccttttgc tttttgcaat gtgtgcatgg taatgccatg
     3901 agaagctaca tccagaatac gctgtgccag tgtatatatt gcatcgatat cagctatgac
     3961 atcctggata ggccgccgtg aaaattgctg tcctttatat gtatggcaga atgcacattt
     4021 gttccatggg caattgcgtg taacacgaat caatatgctg tacgcttcag atggaggcct
     4081 gatgggacct aattcaaatg tgtatggcat ggcaatttct cctgctggat taattagtat
     4141 atcaaaaaaa gaaaaaattt tccgagaaat tgaggggatg ttgataggat tctatgttca
     4201 atgttaaggt gtaggattcg ttagttaggg ttattagaac tttgagaggg tatattcaaa
     4261 aatataacga gatataataa ttatatttat atagatacaa aatactatga attaacccct
     4321 ttttatcctg cctttctcat attttcgtat aatgaattaa acattttttt catcatttct
     4381 tctttgatat ctgaattatg attcaatttc ttataaaaat taaaattgtc ttcaagtagt
     4441 ttttgaagtt cttcattgaa ttttttttca aatgcatacc atacattttc ctttgggttg
     4501 tttttaacaa tttcttcaat atatctatcg ctctttaatc gtgaaagaat agtatctgct
     4561 accactttat cttcatctgt gaattctgtt ccatattttt cgtttatttc ttttacaatc
     4621 attgaaagtg actcaataaa ttcatatttt attccattac ggttttcgtc accgggtttc
     4681 agtttgccac cttcgccatt ttttactttg atgcctttac ttaaatattg aatttttatt
     4741 gattcaagat caacctgttg tgtaacttcc ttaggtaatt tctctttgtc ataaggtaat
     4801 tttctaaaaa gaattttacc aaatacatat aatttttcta aagcagggtc agtaaatgaa
     4861 attaattgtg aaagaaatgc ataaaccctg agatatgact ttaaactagt tctaaatttt
     4921 tcttttttat ttggatcatt ctcttcatta taattcttaa ctacacgatg gataattggc
     4981 acaagatctt cttgtttaaa tttttttgaa aaaaatattt ttacaaaatc ttcaacatct
     5041 tgattggtgt agtatttaaa tgattcaagt gtcaatttaa aatcataaag cttatctgga
     5101 tcggtacctt cttcaagaaa aacatcttca taataattct gaaaagcctg aagaattgta
     5161 tttgaatcat taacaaaatc aatagtgata ggatcttttt ttccgggata tacacggttt
     5221 aatcggctta ttgtttggac agcatttaca ccctgcaatt ttttattaat atacattgca
     5281 tagagtagag gctggtcaaa tccggtttgg aatttgttgg ccactattaa aaacctgtac
     5341 tggctttctt taaaatagct ggctgtttga tcttcaggaa atccattcat tttggcttct
     5401 gaatattcgt tctggctgct atctcctata ttataacttt caataacacc agtaaacgct
     5461 actaaagctt taaagggtaa ttgattttct ttaatgtatt tatcaaaagc caatttatat
     5521 tttactgcct gtgcccttga agagcaaacc accatggctt tggccaaacc gttaatttca
     5581 tgctggcagt tttccataaa atgattaatg ataattgctg ttttcttatc aatagtatgc
     5641 tcgttaagtt caacataagt ttttaataat cggctagctt ttactttatc atattttggg
     5701 tcatcaataa tctttttaac caatttaaaa tatgttgcat atgttgtata gtttggcaaa
     5761 acgtctttaa taaatccttc ctgtatagcc tgcttcatgg tatatatatg gaaaggttta
     5821 tatgacccat caggcatagg tacgccgaat agctcaagag tttttggttt gggggtagca
     5881 gtaaatgcaa aataacttat atttggttgt ttcccacggg atttcatttg ttctatgatt
     5941 tcatcttcat aggttttatc ttcttcatcc gggtcgtctt cggaattcag gttaagaact
     6001 ttcttaaggc tcctggacat ctcgccactg gtagaagaat gtgcttcgtc aataataatg
     6061 gcaaaacgtt tccccggcag ggtttttatt tcttcctcta tatagggaaa tttctgcaag
     6121 gttgtgacaa taatcttggt gcctgcttcc agttcctgtc taagttgttt tgagcctttt
     6181 tcaatacccg caacgacacc agggttctgt tcaaaggctc ggacattggc tcgcaactga
     6241 ctgtcgagaa tacggcggtc ggtaaccaca attatagaat caaagatatt gacatcattt
     6301 ttatcgtgca gattggagag ctgatgcgca agccagctta tagtgtttga ctttccactg
     6361 cccgcgctgt gctgaatgag atagtgctga ccggtgccat tttcataagc atgtttgata
     6421 atattacgaa cacagtccat ctgatgataa cgtgggaaaa ttatcttttt ttcaccagta
     6481 ggttttccat cgtcatcaag tacatcggtt tcctgaatat aatgctgtaa aagttccaga
     6541 aaactgtctt ttaataatac ctctttccag agatattctg ttgaaaaccc cccggtgaaa
     6601 ggattgccgg cttctccatt atttcctttg ttgaaaggta aaaaaagggt tttttgtcct
     6661 ttcaggtggg ttgtcatata tactaattct tcatccagtg caaaatgcac aagacagcgg
     6721 ccatacctga aaagaggttc ctggggatcg cggtcgttct gatattggcg gatagcactt
     6781 tcaacgttgt atccggagcc agtcatgcga tctttaagtt ccattgttac cagaggtata
     6841 ccgtttaaga aaaggaccat gtcaagggag ttttcgttgt gctggctgta ttttacctgc
     6901 cggattacgc tgaacatgtt gttctgatat tttttgcggt agctttcatt aaggccagaa
     6961 gctggtttga aataagcaaa gtcgaaataa acgccatagc tggcaaaggg tttacgaagc
     7021 aggttaagag tgccgcgctt ttctatttcg tctttaatct ttgtaagtag cgcttcctgg
     7081 gctttgtttg ctaactgctt tttatatgct tcccatttat ctttctgtgt agctataatg
     7141 aagttgataa gcatctcggg gtcaaggcat aaagctttat catagtgtga aggattgcgc
     7201 ttaaaatatc cagcatttat taaatcagct tcaatggaag attccatttc tttttcggat
     7261 atattgatca ttacatactc ctaactgtta accatgaaca ccctgaattc tttaaaatta
     7321 tagttgatac ctttaatatt tttcaaggct ttttcttaaa tttatggttc acagtgcatc
     7381 ccttacatcg attttacccg ttaccgcatg agtgatgaga gactgtttat attcatttaa
     7441 aagctcgatc tgcttttcaa tcttgctgac cagtgtatca attttggatg tctcgcggtc
     7501 aagaaaggcg gcgatggctt gttgttcgga gaggggggga aggg
//
LOCUS       DAOSZP010000022         7459 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_8415_length_7459_cov_3.448271, whole genome shotgun sequence.
ACCESSION   DAOSZP010000022 DAOSZP010000000
VERSION     DAOSZP010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 7459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7459
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8415_length_7459_cov_3.448271"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            256..570
                     /locus_tag="PK348_01010"
     CDS             256..570
                     /locus_tag="PK348_01010"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HXK64822.1"
                     /translation="MAEKRKYNRVSKKCKSVVESMEGVTFSTAHDISEGGMFISTPEP
                     VHPGSTVDIEMYISPEETITLKGTVRWVRKHEEGDKKAGMGIQFIPTTQEIEKLKKAM
                     LK"
     gene            complement(562..3300)
                     /locus_tag="PK348_01015"
     CDS             complement(562..3300)
                     /locus_tag="PK348_01015"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HXK64823.1"
                     /translation="MQQVHLPLDEEYISVQDISYSDTAILDYIAQHSFVPNKRFLLKT
                     ESFVLSSAYANNKMLSLSNSRTRILPHQIESTYTVVNSLHKRFLIADEVGLGKTIEAG
                     LIIKELSYRYNYDRICIVCPASLLYQWQHEMKSKFNEDFTIVDKKIYTRYKGNPWVLN
                     KIICSIDFIKNRSFIETLSKVQWDTVVIDEAHRLRRDAATQTKSYQVAEILAKNSKAL
                     LLLSATPFRGKLEELYYLLTLLDKNLLGPVHSFQATYCVPDADLAPLKEKIAQVVIRR
                     TKREVGGFTKRLAKTIRFELYPEERMLYDATTQYVADQFNKAMQTENRAIGFVMTVFQ
                     KLLDSSSYALLCALKKRHEYLTGILVQKKASAYDDSVLDEDAIEDVDTVVQVESVELE
                     EELTTLAKLIAMAQDVTRNKKAEKLVQLVKKLKAQNHKKILIFTQFRTTQDYLAQVLK
                     SYKVVLFHGSLSREEKEQAIECFKSESEILICTEAGGEGRNLQFCNVLINYDLPWSPL
                     RVEQRIGRIHRFGQPQNVIIYNFATKNTVAERVLEVLNHKLKLFEESLGTPDVILGDI
                     ADERFFNSIFMELIAGLKSKKRVTKDIDTHIQKAKSSYSKLQELTVAKKIDLNFDEYY
                     KITMQERAFSNKRIEQFINELKEVDASISNYISGPNPKNKLYFVRYNPVTHTPVNRYG
                     TFTSEVALENESLEFLAFGHPVIDFCIEQCQEHAFGGLAGIKRIDCPYDCSGLLFHYL
                     VTLKAKEEVQRIIPVFVSKDILNQSIIDDIEQETLRQYGCAVSVADDVAREYSSNIYH
                     YYELSLKRLEKKVGAIVTEIKDNMNLHIDPYMERVCQHYQQEKEYLHKRLEWQECQQK
                     WYGKDMQSAITRTKNMIQKIDEEHHNRLKQYSGFTDVTWDASLVNAGILVCTPGSF"
     gene            complement(3451..6441)
                     /locus_tag="PK348_01020"
     CDS             complement(3451..6441)
                     /locus_tag="PK348_01020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK64824.1"
                     /translation="MAVTEEIQNLTPEEEAEITRIVDSFDAELASIAQMRGRVSVIQA
                     DSSAADFTITPGQREEEGEGLTTKEVYEESEEIVDITDMIEEVPEEMPPSVEEALPEF
                     EEHVSEEALPQEPEKAFEETLPEELPEEIPSFEEKELPPKPPKKEVSPLDELEELTKF
                     EPETVEAHEIRTDEFVTEEPRIPRDVSLDEDIHLSEIGTEEVSEISEIKAEELPEADL
                     TTLGLDEEIPEPSAFEEVSKEEAPRARFETPSFDELESFEEPVRKAEKVESVVEEFRP
                     EFEGLEEEEPQPISMEESVEISDAELRRLKKAMLLLHPVLRRKIKEIILRDELSPEDT
                     RKLIDLILSGKPEDNIQRFIEKKLNIKLTLEEEIPHRRVVTARPEYTREGMQRQQRLL
                     RQTKIIAAAVLIGFALTVTVYQFIYKPFMAKRLINKGVALILEPGDPATKKVKDYKKA
                     EEYFEYVDQHYKKDYLFGYNAYARAYFKKREFDYAYSKLKRAFEINSRDIDTLNNTGY
                     FFAKIPQNYYSRIQNDALRTFAPKGTYEMSQVDMAIMMYNRVLAIDPKNITAMFGVGN
                     AYLYQGQYLKSRQYFENILKVDPNSWVGYAGLLNLFIERDNLPEVLSIHTDLRARDIL
                     TDIPSPLLAKLAHYYLGKNRSDTFNVRVDLGIQSPRLKDEEDNLVPACRGVLDALKEK
                     DPDYPPLYVASAKLAAAQKNLKLVEMLLKEAEQKAEEKGERYAAALQLLGEYNYTIKQ
                     PVEAYKYLNAAITAYQYPPEFTYEDFYYESESIGKAYAYLGHIFYYFFDKVKFRFGDE
                     LDEALLDEDADKMANLDIARQKYELAIQENFFSPEVRYNLGRIYYVNQNYQQALEQWL
                     NLYEDIVTNPQVMFAIGNAFYHMNNLEASKGEYLKMIAVFERKAESIRLPRPERKEHR
                     IIFQSLASAYNNLGAIYQIQGSETKSSIAYWKAIDYASRLGMENEFARVNLARGFKPR
                     TTPIMPILDENIPYSIDIFRADMR"
     gene            6646..7377
                     /locus_tag="PK348_01025"
     CDS             6646..7377
                     /locus_tag="PK348_01025"
                     /inference="COORDINATES: protein motif:HMM:NF027875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen-binding domain-containing protein"
                     /protein_id="HXK64825.1"
                     /translation="MPKPLQWIMLIISLCYFSSSDYSPAKSVPYYSLYTLSKAMPPSP
                     VTVSHSKPGMPQILRGTLITYKNRSAHRVYIAGDFSYWKKQPMERSNNGVWFYIIPFN
                     DDDRVVRYKFLVDDVWINDPLNHDIADDNAGSYVSIVRQDKLSEGTQVSYKILDESIR
                     LVEFRLYAPKASIVALAGDFNNWNPEVDLLQKDKNGIWRLQKRLPKGAFRYNFVVDGI
                     WKLDLYNPHTASNAAGMLCSVIQFQ"
BASE COUNT         2071 a         1631 c         1383 g         2374 t
ORIGIN      
        1 gccatttagt tttaaccggc agtgtaatgc cacaatgagg aaacaacact ttgttgtagg
       61 aataaaaaat gcgcaaggta accacccaat tgtacttcat aaaattacgt atacagtaaa
      121 accactgtca acatttgcaa tcattttatt tagtgtagat atataaataa cgctatcaaa
      181 aaaatttatt gcaataatat tacacatact atacaataaa gccattcaac acaacaccac
      241 acaagcgagg tatctatggc cgaaaagcga aaatataacc gtgtgtcaaa aaaatgcaaa
      301 tcagtggttg aaagcatgga aggcgtaacc ttcagcaccg cccacgatat cagcgaaggc
      361 ggcatgttta tatcaacacc cgagccggtt catcctggta gcactgttga tattgaaatg
      421 tacatttcac ctgaagaaac cattacacta aaggggacag tacgctgggt acgcaaacat
      481 gaagagggtg ataaaaaagc tggcatggga attcaattta tacctaccac tcaggaaatt
      541 gaaaagctca aaaaagccat gttaaaatga acccggtgtg cacaccagta ttcctgcatt
      601 caccagactg gcatcccacg ttacatcagt aaatccagaa tactgcttca ggcggttgtg
      661 gtgttcttcg tcaatttttt gaatcatatt ttttgttctg gtaatggcgc tttgcatgtc
      721 tttgccgtac cacttctgct gacattcctg ccactccagg cgtttatgca gatactcttt
      781 ttcctgctgg taatgctggc aaaccctttc catatacggg tcaatatgca gattcatgtt
      841 gtcttttatt tcggtaacta tcgcccccac ttttttttca agtcttttaa gcgatagctc
      901 atagtaatga tatatgttgc tggaatactc ccttgctaca tcatccgcaa cgctcaccgc
      961 gcacccatac tgacgcaagg tctcctgctc aatatcatca atgatagatt ggttgagaat
     1021 atcctttgac acaaacaccg gtatgatgcg ctgcacctct tcctttgcct tcagggttac
     1081 aaggtaatgg aataaaagac cactacaatc atagggacaa tcaatccgct tgatgccagc
     1141 aagtccgcca aatgcatgct cttgacattg ctctatacag aagtcaataa ccggatgtcc
     1201 aaaagccaga aattccagtg actcattttc cagcgccacc tcactggtaa atgttccata
     1261 tcggttcaca ggcgtatgcg ttacggggtt atagcgcaca aagtataatt tatttttggg
     1321 atttggccct gaaatataat tgcttataga agcatcaact tcttttaatt cattgataaa
     1381 ctgctcaatg cgtttgtttg aaaacgcacg ttcctgcatg gtaattttat aatactcatc
     1441 aaaattaaga tcaatttttt ttgcaacggt cagttcctgc aatttactgt agcttgattt
     1501 agctttttgt atgtgggtat caatatcttt cgttactcgc tttttgcttt tcagccctgc
     1561 aatcaattcc ataaagatgc tattaaaaaa ccgttcatcg gcaatatcgc caagaatgac
     1621 atctggtgtg cccagcgatt cctcaaaaag tttaagctta tgattcagca cttcaagtac
     1681 gcgttcggca acagtgtttt tggttgcaaa gttatagatg atgacattct gcggctgccc
     1741 aaagcggtga attctaccaa tgcgctgttc cactctaagt ggcgaccacg gcagatcata
     1801 gtttatcagc acattgcaaa actgcagatt gcgcccctca ccaccggctt ccgtacatat
     1861 taatatttca gattcacttt taaaacactc tattgcctgc tccttttctt ccctgcttaa
     1921 tgatccatga aacaggacaa ccttatagct ttttaaaacc tgtgccaggt aatcctgtgt
     1981 ggtcctgaat tgtgtaaaaa ttaaaatttt tttgtggttc tgtgctttca atttttttac
     2041 aagctgcaca agcttttcgg cttttttgtt tcgtgttaca tcctgtgcca tagcaataag
     2101 ctttgcaagc gttgtcagtt cctcttcaag ctcaacactt tcaacctgaa ctactgtatc
     2161 aacatcttct attgcatcct catctaacac gctgtcatca tatgcactgg ctttcttctg
     2221 caccagaatg ccggttaaat actcatgacg ttttttcaat gcacacagca gcgcatacga
     2281 tgatgagtcc agcaatttct gaaacaccgt cataacaaat ccaatggcac gattttccgt
     2341 ctgcattgct ttattaaact ggtctgctac atactgtgtg gttgcatcat acaacatccg
     2401 ctcctcagga tacaattcaa agcgtattgt ttttgccaga cgttttgtaa agcctcccac
     2461 ctcacgcttt gtacggcgta taaccacctg cgcaattttt tctttaagtg gtgcaagatc
     2521 agcatctggc acacagtagg ttgcctgaaa gctgtgtact ggccccaaca gatttttatc
     2581 aagaagtgtt agcagataat acagttcctc aagctttccc cgaaacggcg ttgccgatag
     2641 caaaagcagc gctttgctgt tttttgccag tatctctgcc acctgatatg attttgtctg
     2701 agtggccgca tcacggcgca ggcgatgtgc ctcatcaata acaaccgtat cccactgaac
     2761 tttcgataac gtttcaataa agctgcggtt tttaataaaa tcaatggagc agattatttt
     2821 atttaaaacc catggattgc ctttataccg cgtatatatt tttttatcaa caatggtaaa
     2881 gtcttcatta aacttgcttt tcatctcgtg ctgccactgg tacagcaaac ttgcagggca
     2941 tacaatacaa atcctgtcat aattatacct gtacgatagt tctttaataa taagacctgc
     3001 ctcaatagtt ttgccaagcc ccacctcatc agcaattaaa aatcgcttgt gcagtgaatt
     3061 gaccacagtg taggtgcttt caatctggtg gggaagtatc cgtgtacgtg aattagatag
     3121 cgacagcatc ttgttattgg catacgcgct tgaaagcaca aagctttcgg ttttaagcaa
     3181 aaaacgctta ttaggcacaa acgaatgttg tgctatataa tccagaattg cagtatcaga
     3241 gtatgaaata tcctgcacac tgatatattc ttcatctaat ggtagatgaa cttgttgcat
     3301 atagattcaa ttccttgtat atatgattta ttctttttat ggtataggat agtccaatgt
     3361 gacataatat acaaatagct cgttattaca agcatttttt atacgtatcg tattccctat
     3421 tcagttgtgt gtggaaattt atattttata ttaccgcatg tcagctctga aaatatcaat
     3481 actgtagggg atattttcat ctaagattgg cataattggt gttgtacggg gtttaaatcc
     3541 acgtgccagg ttaacacggg caaattcatt ttccatgccc aggcgtgaag cataatcaat
     3601 tgctttccag tatgcaatac tgctttttgt ctcactaccc tgaatctggt atatggcacc
     3661 aagattatta tatgctgatg caagtgactg gaatatgata cggtgctctt tgcgctccgg
     3721 ccgtggcaat cgtattgact ccgccttgcg ttcaaatacg gcaatcattt tcaggtattc
     3781 ccctttgctt gcctctaaat tattcatatg atagaacgcg ttgcctatcg caaacataac
     3841 ctgaggatta gtaactatat cctcatacag attaagccac tgttccagcg cctgctgata
     3901 gttctggtta acataatata ttctgccaag gttataccga acctcaggcg agaaaaaatt
     3961 ttcctgaata gccagctcgt acttctgccg cgcaatatct aagtttgcca ttttatcagc
     4021 atcttcatca agaagcgctt catcaagctc atcgccaaac ctgaatttta ccttatcaaa
     4081 gaaataataa aagatgtgac ccagatatgc atacgctttg cctattgact cagactcata
     4141 gtaaaaatcc tcataggtaa actcaggcgg gtattgatag gcagttattg ctgcattgag
     4201 gtatttgtag gcttcaaccg gctgtttgat tgtgtagttg tattcaccca gaagctgcaa
     4261 tgctgctgca taccgttccc ctttttcctc agctttttgc tcagcttctt ttaaaagcat
     4321 ttccacaagc ttcagatttt tttgtgctgc agccagcttg gcgcttgcaa catacaacgg
     4381 cggataatca gggtcttttt ctttcaatgc atcaagcaca ccacggcatg ctggcaccag
     4441 gttatcctct tcgtccttca ggcgtggcga ctgtatacct aaatcaacac gtacattaaa
     4501 cgtatccgaa cggtttttac ccagatagta atgggccaat tttgccagca gtggcgaagg
     4561 gatatctgtt aaaatatcac gcgcacgcag gtcagtatgg atggatagca cttcgggcaa
     4621 attatcacgc tcaataaaaa gattaagaag accagcatag ccaacccatg aattggggtc
     4681 tactttcaga atgttttcaa aatactgccg tgattttaaa tactgcccct gatacagata
     4741 tgcattgcct acgccaaaca ttgctgtaat attttttggg tcaattgcaa gcaccctgtt
     4801 gtacatcatt attgccatat caacctgtga catctcatag gtgcctttgg gtgcaaatgt
     4861 ccttagtgca tcgttttgta tgcgactata atagttttgc ggtatttttg caaagaaata
     4921 gcccgtgtta ttcagtgtgt caatatcacg actgtttatt tcaaatgctc gtttcaactt
     4981 gctgtacgca tagtcaaact ctcgtttttt aaagtatgcg cgtgcatatg cattgtatcc
     5041 aaatagataa tcttttttat aatgctggtc aacgtattca aaatattctt ctgccttttt
     5101 ataatccttt acttttttgg ttgcagggtc acccggctcc agaataagtg ccacaccttt
     5161 attaataagc ctttttgcca tgaaaggctt ataaataaat tgatagacag ttactgttaa
     5221 tgcaaatcca atgagtaccg ctgctgcaat aatctttgtt tgccgcagca atcgctgctg
     5281 tcgctgcatc ccttcacggg tatattcagg cctggcagtg acaacccttc gatgtggaat
     5341 ttcttcttca agggtaagtt taatattgag tttcttttca ataaaccgtt gaatattgtc
     5401 ttcaggtttg cccgatagta tcaggtcaat aagctttcgg gtatcttcag gagataactc
     5461 atcccgtaaa attatctctt ttatttttct gcggagaacg ggatgaagta gcagcattgc
     5521 tttttttagc cgccgcaact cagcatcaga aatttcaacg ctttcttcca ttgaaattgg
     5581 ctgtggctct tcctcttcca ggccttcaaa ttcaggcctg aattcttcaa ctacgctctc
     5641 aaccttttca gcttttctta ccggttcttc aaagctttca agttcatcaa atgaaggagt
     5701 ttcaaaacga gcccgtggag cttcttcttt tgaaacttcc tcaaatgctg atggttcagg
     5761 gatttcctca tcaagtccta atgttgtaag atcagcttca ggaagttctt cagctttaat
     5821 ttcagaaatt tcagatactt cttcagtgcc aatctcagaa agatgtatat cctcatctaa
     5881 actgacatca cgggggatac gtggttcctc agttacaaat tcatcggtac ggatttcatg
     5941 ggcttcaaca gtttcgggtt caaattttgt aagctcttcc agttcatcca gtgggcttac
     6001 ttctttcttt ggtggctttg gtggcagttc cttttcttca aacgagggga tttcttctgg
     6061 caactcttca ggcaacgttt cttcaaaagc tttttcaggt tcctgtggca atgcctcttc
     6121 tgaaacgtgc tcttcaaatt caggaagcgc ttcttcaact gaaggtggca tttcctcagg
     6181 cacttcttct atcatgtcgg ttatatctac tatctcttca gactcttcat atacttcttt
     6241 tgtagttaag ccttctcctt cctcttcacg ttgcccggga gtaatggtaa aatcagcagc
     6301 tgatgaatca gcctgtatca cgcttaccct gccacgcatc tgagcaatgg atgcaagttc
     6361 agcatcaaat gaatcgacta ttcgcgtgat ttcagcttct tcttccggag ttaaattttg
     6421 tatttcttcg gttaccgcca taatgggctc tcgttatctt tgattatcct gaataatgtg
     6481 acataaacat actaatcata ttctgtttaa aaaatcaaac atattttgcc aattatatat
     6541 taatatgcat tttgaataaa atcatataaa tattttctct atacctatcg acatattaat
     6601 atgtaattta taagcgtaaa atttagccac aggagcccat agtacatgcc aaaaccgctg
     6661 caatggatta tgctcatcat ttcactgtgt tatttttcca gtagtgatta ttctccagca
     6721 aaaagcgttc cttattattc actgtacacc ctttcaaagg ctatgccacc ttctccagta
     6781 actgtctccc atagtaaacc gggaatgcca cagatactac gggggacttt gattacctat
     6841 aaaaaccgca gtgctcaccg ggtatatatt gccggtgatt tttcatactg gaaaaagcag
     6901 cctatggagc gaagtaataa tggcgtctgg ttttacatca ttccctttaa tgatgatgat
     6961 cgtgtagtgc ggtacaaatt tctggttgat gatgtgtgga ttaacgatcc tttaaatcat
     7021 gatattgctg atgacaatgc aggttcatat gtttccattg tgcgccagga taaactgtct
     7081 gaaggcacac aggtaagtta caaaatactt gatgaaagta tacggctggt ggaattcagg
     7141 ctgtatgcac caaaagcatc tattgtggca cttgccggcg atttcaacaa ctggaacccg
     7201 gaagttgatc tgctgcaaaa agacaaaaat ggaatatggc gtctgcaaaa gcgactacca
     7261 aaaggagcat tccgctacaa ttttgttgtg gatggtatct ggaagctgga tttatataat
     7321 cctcatactg catccaatgc agcaggaatg ctctgttcag ttatacagtt tcaatgaata
     7381 caaatatggt ttttattctg gcgatagtta ctgaaaaact ataatccttt aatgacagta
     7441 gcaagtgcta agataacaa
//
LOCUS       DAOSZP010000023         7381 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_8543_length_7381_cov_3.390800, whole genome shotgun sequence.
ACCESSION   DAOSZP010000023 DAOSZP010000000
VERSION     DAOSZP010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 7381)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7381)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7381
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8543_length_7381_cov_3.390800"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..987)
                     /gene="groEL"
                     /locus_tag="PK348_01030"
     CDS             complement(<1..987)
                     /gene="groEL"
                     /locus_tag="PK348_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004751305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HXK64826.1"
                     /translation="MAKILQYNDEARRSIYQGVIKLSDAVKVTLGPRGRNVVIDKKFG
                     SPTITKDGVTVAKEIELEDPFENMGAQLVKEVATKTNDVAGDGTTTATILAAAIYHES
                     LKNVTAGANPMSLKRGIDKAVEAAVKFIGDTAREIKDKKEIAQVAAISANNDMEIGEL
                     IAEAMDKVGKDGVITVEEAKSIETHLEVVEGMQFDRGYISPYMVTDPENMEAVLEDAF
                     ILINEKKISTMKDLLPILEKVAQAGRPLLIIAEDVEGEALATIVVNTLRKVIQCVAVK
                     APGFGDRRKAMLEDIAILTKGQVISEDLGLKLENATLDMLGRAKKVIVDKENT"
     gene            complement(1021..1305)
                     /gene="groES"
                     /locus_tag="PK348_01035"
     CDS             complement(1021..1305)
                     /gene="groES"
                     /locus_tag="PK348_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HXK64827.1"
                     /translation="MGIKPLGDRVLIEVMEEELPKQGSLYIPDTAKEKPQQGKVLAVG
                     KGRYEDGKWIPLDVKVGDTVLFGKYAGTEVKYGGKEYLIVRENDILAIFE"
     gene            complement(1597..2751)
                     /locus_tag="PK348_01040"
     CDS             complement(1597..2751)
                     /locus_tag="PK348_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013681688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXK64828.1"
                     /translation="MREVIDQLELTYQRIKLFIPERKRPFFRTLDTENIRSFLLYGPR
                     GVGKTTFLIDTIKDKNFLYLSADSLAIHAYPLYDIIKEIFARGYDGVVVDEIHFSNNW
                     SMHIKNVYDDYPDKYIWISDSSNLILQKGMGDLSRRFVQYRMPMLSFREYVYLTEGIY
                     YDPVDVFSNYEQLLHIIKGINVIKLFKDYITGGLRPIFFEGQYCNRLKSLLEKSIYYD
                     IPFYVPSIQENHLQLMNAIVSHLLTSPIPTINISGMCSDWGVSKDKLYSLLYVMDKSE
                     LIKIVQKKGNKHIYSKGGKIFLGDPSMYYCFNGNIGSAREAFIVFSLGEKYPIFASDN
                     EKEYDYQVGRYTIEVGGRNKKNKKADFVIKDDIDIPYKNNFPLWMIGFCF"
     gene            complement(2875..3342)
                     /gene="moaC"
                     /locus_tag="PK348_01045"
     CDS             complement(2875..3342)
                     /gene="moaC"
                     /locus_tag="PK348_01045"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013007208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HXK64829.1"
                     /translation="MDFTHYTDGKPVMVDVGGKEVTSRIARAAGCVYMQKETIEKIQK
                     NLIPKGNPFDVARVAGILAAKKTHELIPMCHPLMITHVDIAIVCDDNNSRVVIEATVR
                     CDGKTGVEMEALTAVSVAALTIYDMCKAVDKQMVIGDIRLVEKHGGKSDYVEK"
     gene            complement(3357..3755)
                     /locus_tag="PK348_01050"
     CDS             complement(3357..3755)
                     /locus_tag="PK348_01050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK64830.1"
                     /translation="MKKIVYVVVLLLLCNISAFAKDFLSNQDSRDRFIRLEVIVDEGF
                     KSTNIRIDNLRDDIKDIKTFMLWGFGILFSGMGILIGFVLWDRRTALAPVIKKYEELE
                     ERQEKIEHSLKELAIQDKKVGDTLRKVGLL"
     gene            complement(3953..4666)
                     /gene="pssA"
                     /locus_tag="PK348_01055"
     CDS             complement(3953..4666)
                     /gene="pssA"
                     /locus_tag="PK348_01055"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015678810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HXK64831.1"
                     /translation="MSVAWIPNTLTLGNLTLGFISLIFASMNIPHHLTIAGILILAAA
                     LLDGLDGQVARMLGVASELGKELDSLADCVTFGVAPGFLAYKGYLQGTWVQAFGQSID
                     AGVFIAAIFPVCAAYRLARFNVTHVPNAFDGLPSPIAGIIVALVPLCFLNIHIPKIVF
                     IISFIVVALLMVSTVRYSKPQSYLLEKITGIKLFLFIIVFIALIVIFKQWVILAIIAL
                     YILSGILSFIIQVIQDYRY"
     gene            complement(4756..5436)
                     /locus_tag="PK348_01060"
     CDS             complement(4756..5436)
                     /locus_tag="PK348_01060"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HXK64832.1"
                     /translation="MSYRHNNTTVLHCEAVCFDMDGVVADSMHAHAQCWIDVFREYGV
                     HLDKTDIFKREGMTGLESIADIFRDKGFAMPPEHILKDLQKKKLQLFSNYQIEIYPQM
                     YSIFPLLKSKGKKIGIVTGSFRYLVFELIPQELMHYVDVIISVDDVTKGKPNPDPYIE
                     AVKKLQVKPEHVIVVENAPMGIKAAKAAGLFCIAISTTLGAGFLKEADVIMQDHNELV
                     AYFTHCLS"
     gene            complement(5459..6616)
                     /locus_tag="PK348_01065"
     CDS             complement(5459..6616)
                     /locus_tag="PK348_01065"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF002528.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HXK64833.1"
                     /translation="MNKKSGGNSPVKKTTGKKLSLGIIYGGKSGEHDVSRCSAASVAL
                     VASKKYRVVCIGITRQGLWYVQDGINVETHPSFGQVLSLKETGTWLINMHSATDTLEL
                     YNKKTGQTVRTEVVFPLVHGTYGEDGTLQGLLDMVGVPYVGPGVLGSAVGMDKDIAKR
                     VIQHSGLVAIVPFETVRTDDMQSSDDMLLQKIAAMLPVFVKPANAGSSVGISKVTTIP
                     DLQKALHKAFAFDSKVIVEKAVDCREFEVAVMGNEKPRASAVGEVIPQHEYYSYEAKY
                     VDKDGAKLAIPANIPDALALKLRQAACSVYTMLECEGMARVDFFVDKHDGTIYFNEVN
                     TLPGFTSISMYPKLWEYSGVSYPKLIDTLISYAFRRHRVKKKMVKAFLSMK"
     gene            complement(6609..>7381)
                     /locus_tag="PK348_01070"
     CDS             complement(6609..>7381)
                     /locus_tag="PK348_01070"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002721684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HXK64834.1"
                     /translation="RGGSDTSAVALATVLGTRDCEIYTDVDGVFTADPRIVSNTKKLN
                     EISYEEMLELARLGAGVLHSRSVEFAKKYSIRLHVRSSFNDNEGTIVMPREEMMEKFV
                     ISGVTSKDDEAKITIRDIPDRPGVASLLFGELGKQKVYVNMIVQSTGKDGKASISFTV
                     LKNDLDKALKICESLKEKLGASGIDYKENIAIVSAVGVGMLSSWGVAGRMFDVLSKHN
                     INIEMISTSEIGISCVIDAMYTELAVKAIHKEFIESNE"
BASE COUNT         2296 a         1619 c         1105 g         2361 t
ORIGIN      
        1 agtattttct ttgtcaacaa taaccttttt tgcacgacca agcatatcaa gtgttgcatt
       61 ttcaagcttg agtcccagat cttcagaaat tacctgaccc ttggtcaaga ttgcaatatc
      121 ttcaagcatt gctttcctgc gatcgccaaa acctggggct tttactgcaa cacactggat
      181 aaccttacgt aatgtgttaa caacaatggt agccagtgct tcgccttcaa cgtcttcagc
      241 aataatgagt aatggtcttc ctgcctgagc aactttctca aggattggaa gcaggtcttt
      301 catggttgag attttctttt cgttgatgag gataaatgca tcctcaagta ctgcttccat
      361 attttctgga tcggttacca tgtagggaga aatgtaaccg cggtcaaact gcataccttc
      421 aacaacttca aggtgagttt caatggactt ggcttcttca acagtgatta caccgtcttt
      481 gccaacctta tccattgctt cagcaatcaa ctcaccaatt tccatatcat tgttagcaga
      541 aatagctgct acctgagcta tttctttttt atcttttatt tcacgtgctg tatcaccaat
      601 aaattttact gctgcttcaa ctgctttgtc aatgccacgt ttcagactca ttgggtttgc
      661 accagcagtt acattcttta atgattcatg atagatagca gcagctaaaa tggttgcagt
      721 ggttgttcca tcaccggcaa catcatttgt cttggtagca acttccttaa caagctgagc
      781 acccatattt tcaaatgggt cttcaagttc aatttctttt gctactgtaa caccatcttt
      841 ggtaatagtt ggcgagccaa acttcttgtc tataacaaca tttctaccac ggggaccaag
      901 tgttaccttc actgcatctg ataacttaat cacaccctga tagattgatc gccgtgcttc
      961 atcattatac tgtaaaatct ttgccatgtt cttcaccttc cttttctaca atgtaatttt
     1021 ttactcaaaa atagccagaa tatcattttc gcgaacaatc aaatactcct tgccaccata
     1081 cttaacttct gttccggcat atttgccaaa cagtacagta tcaccaacct taacatctaa
     1141 tggaatccat ttgccatcct catagcggcc cttgccaaca gcaagaacct tcccctgctg
     1201 tggtttttct tttgccgtat cggggatgta aaggcttccc tgctttggaa gttcttcttc
     1261 catcacttca atcagaactc gatctcctaa tggttttatt cccacggtac tcctccttaa
     1321 ccttgatttt aattttaata tgataattgt aatctaaaaa tactgaatta tgattctttt
     1381 ttaagcctat tgtactgaga aaataagaag tgttgatatt aattgttagc actcttttta
     1441 gtgagtgcta acaattaata atatagcaaa aaaaaatttt ataacaattt taattatgag
     1501 gaattattat taattattgc tttttataga cttttatcca aaaatatgat atcgaaatat
     1561 cagaaataaa aaatattggt ctaaataaaa aaatttttaa aaacaaaaac caatcatcca
     1621 taatggaaaa ttatttttat aagggatatc aatatcatct tttataacaa aatcggcctt
     1681 tttgtttttc ttatttctcc ctcctacctc aatggtatac ctacccacct ggtaatcata
     1741 ttccttttca ttatccgatg caaatatagg atacttttca cctaaagaaa atactataaa
     1801 tgcctcccgt gcactaccaa tatttccatt aaaacaataa tacattgaag gatcacccag
     1861 aaatattttg ccacctttgg aataaatatg cttattacct tttttttgta ctattttaat
     1921 taattctgat ttatccatta catataataa agaatataat ttatctttac taacgcccca
     1981 atcactacac attccagaaa tattaatagt agggatggga gaagttaata aatggctaac
     2041 aatagcattc ataagttgaa gatgattttc ctgaattgaa ggaacataaa atggaatatc
     2101 ataatatatt gatttttcta gtagtgattt caatctatta caatactgac cctcaaaaaa
     2161 aattggcctc aaaccacctg taatataatc tttaaataat ttgataacat taatcccttt
     2221 aatgatatgc aacaattgtt cataatttga aaaaacatct acaggatcat aatatatgcc
     2281 ttcagttaaa tatacatatt ctctaaaaga caacattggc attcggtatt gtacaaacct
     2341 ccttgataaa tcacccatcc ctttttgcaa tataagatta ctgctatcac taatccatat
     2401 atatttatcc gggtaatcat catatacatt tttaatatgc atactccagt tattgctgaa
     2461 atgaatttca tcaaccacaa ccccatcgta accacgagca aatatttctt taataatatc
     2521 ataaagagga tacgcatgta ttgcaagact atcagcagat aagtataaaa aattcttatc
     2581 tttaattgta tcgataagaa aagttgtttt gccaacaccg cgaggaccat acagcaggaa
     2641 agatcgtata ttttcagtat ctaatgtcct gaaaaatggc cgttttcttt ctggtataaa
     2701 aagcttgatt cgctgatatg tcagttctaa ttgatcaatt acttctctca tagttctatt
     2761 atagaactat atatacataa aatcaactat taattttaaa ttttttcaaa tattttataa
     2821 aattcgttct atattagaac tatttttgat taaatagttc tgaaatagaa ctatttattt
     2881 ctctacataa tcacttttac cgccatgctt ttcaacaagc cgtatatcgc caataaccat
     2941 ctgtttatcc actgctttgc acatgtcata gatagtcaaa gctgcaacac tgactgccgt
     3001 taaggcttcc atctccacac cggttttgcc atcgcatcgt actgttgctt caatgacaac
     3061 ccgcgaatta ttatcatcac aaactatggc aatatccaca tgggttatca taagcggatg
     3121 gcacataggg ataagttcat gggtcttttt tgcagcaagt attccagcta ctctggccac
     3181 atcaaaagga ttgcctttgg ggataaggtt tttctgtatt ttttcaatgg tttctttctg
     3241 catgtaaaca caaccggctg cgcgggcgat acgagaagtt acctccttgc ccccaacatc
     3301 aaccatgacc ggtttaccat ctgtataatg tgtaaaatcc attgtttcta tattttttac
     3361 aataatccaa ccttcctcaa tgtatcgcct acttttttat cctgaatagc caattccttt
     3421 aaagaatgtt caattttctc ctgcctttct tccaattctt cgtatttttt tataactgga
     3481 gccagtgcag ttcgtctgtc ccatagaaca aatcctataa gaatacccat cccgctgaaa
     3541 agaattccga atccccataa cataaatgtt tttatgtctt ttatatcatc ccgtaaatta
     3601 tctattctta tatttgttga tttaaaacct tcatcaacaa ttacttctaa acgtataaac
     3661 ctatctctac tatcctgatt ggataaaaaa tcttttgcaa aagcagaaat attacataat
     3721 aataataaaa caactacata tactattttt ttcattatta acaatcaccc ctcatgtaat
     3781 cgccatcaaa aatctttata aaataaagaa cctttgctgt aaacaaaaca aatatacaaa
     3841 aaaaacatca attcagcaaa tattccgcta acctcagtgg ataaacaact actgaagctt
     3901 taagcatttt taaaaataat ttttaatatc catataacat aaatgaaatc aactaatacc
     3961 tgtaatcctg tattacctgt ataataaaac tcaatatccc tgacagtata tacagggcaa
     4021 taattgccag tataacccac tgcttaaata ttactataag ggcaataaaa acaataatga
     4081 acaggaataa cttaatgcct gtaatttttt caagaaggta tgactgcggt ttggaatacc
     4141 tcactgttga aaccataaga agcgcaacaa ctatgaagct aataataaat actattttgg
     4201 gaatatgtat atttaaaaag cataatggaa caagtgcaac tattataccc gctattggtg
     4261 aaggtaagcc atcaaatgca ttggggacat gtgtaacatt aaaccgtgct aaacggtatg
     4321 cagcacatac agggaatatt gctgcaataa aaacaccggc atcaattgac tgcccaaaag
     4381 cctgaaccca ggtaccctgc aaataaccct tatatgctaa aaagcccggc gctacaccaa
     4441 aggttacgca atctgccagt gaatccagtt ccttacccag ttcacttgca actcccagca
     4501 tacgcgcaac ctgcccatcc aggccatcaa gaagcgctgc agctaaaatt aaaattccag
     4561 ctattgtcag atgatgagga atattcatgc ttgcaaaaat taatgagata aaccccaatg
     4621 taagattacc taatgttaat gtatttggaa tccatgcaac actcatatca cacctatgca
     4681 ctatagtatt tttcaaaacc aaacaatgtt gctattatat gattatttgc cagcctgtca
     4741 atgaaaaaac agctttcatg ataggcaatg cgtgaagtat gcaactaatt cattgtgatc
     4801 ctgcattatg acatcagctt ctttaagaaa cccagccccc agtgtggtac tgatggcaat
     4861 acagaacaag cccgcagctt ttgcagcttt tatgcccata ggtgcatttt ccaccacaat
     4921 gacatgttca ggctttacct gtaacttttt aactgcttct atatacgggt cagggtttgg
     4981 ttttcctttt gtaacatcat caacgctgat gattacatca acatagtgca tcagttcttg
     5041 aggaataagc tcaaaaacaa gataacggaa agaccctgta acaatcccaa tctttttccc
     5101 ttttgacttt aacaaaggaa aaatagaata catttgcgga tatatttcaa tctgatagtt
     5161 tgaaaacaat tgtaattttt tcttctgtaa atctttcagt atatgttcag gcggcatggc
     5221 aaacccttta tcacggaaaa tatcagctat tgactccagc cccgtcatgc cttcacgctt
     5281 aaaaatatca gttttatcca gatgaactcc atattccctg aatacatcaa tccagcactg
     5341 tgcatgcgca tgcatggaat cagcaacaac accatccata tcaaagcaca cagcttcaca
     5401 gtgtagtact gttgtattgt tgtggcgata gctcatagta tcaattcaca ggttgctatt
     5461 atttcattga aaggaaggcc ttcaccatct tcttctttac acggtggcgc ctgaatgcat
     5521 acgaaatgag tgtatctata agtttggggt aagatactcc agaatattcc cacaattttg
     5581 gatacatgct tatactggta aacccgggca atgtattgac ttcattgaag tagattgtcc
     5641 catcatgttt atccacaaaa aaatctaccc gcgccattcc ttcgcattca agcattgtat
     5701 acacagaaca tgctgcctga cgtaacttca gtgccagtgc atcaggaata tttgctggta
     5761 tggcaagctt agcaccatct ttgtcaacat atttggcttc atacgaatag tactcatgct
     5821 gcgggataac ctcacccact gctgaagcac gtggtttttc attgcccatg acggcaacct
     5881 caaactcacg gcagtcaaca gctttttcta ctattacttt tgagtcaaac gcaaaggctt
     5941 tgtgtagggc tttttgcagg tccggtattg ttgttacctt tgatatacca acagatgaac
     6001 cagcattggc aggtttcaca aacacaggta gcatggcagc aatcttttgt agcagcatat
     6061 catcagatga ttgcatatca tcagtgcgaa ctgtttcaaa aggaactatc gccaccaaac
     6121 ctgaatgttg tattacccgt ttggcaatat ccttatccat acccacagct gaacccagaa
     6181 cacctgggcc aacgtatggc acaccaacca tatcaagcaa accctgcaat gtgccatcct
     6241 cgccatatgt gccatgtacc agtgggaaca caacttcagt gcgtacagtt tgtcctgttt
     6301 ttttattata gagttctaac gtatcagtgg cactgtgcat attgataagc cacgtgccgg
     6361 tttctttaag cgatagtacc tggccaaacg atggatgtgt ctctacatta ataccatcct
     6421 gtacatacca cagaccctgc cgcgttatac caatgcacac caccctgtat ttttttgatg
     6481 caacaagcgc aaccgaagct gctgaacagc gcgaaacgtc atgttcacca cttttgccac
     6541 cgtagataat accaagacta agtttcttgc ctgtggtttt ttttacgggg ctattgccac
     6601 cacttttttt attcattgct ttcaataaat tccttgtgaa tggcctttac tgctaattcg
     6661 gtgtacattg catcaataac acatgaaatg cctatctcag atgttgaaat catctcaatg
     6721 tttatattat gctttgacag tacatcaaac atacggcctg caacacccca cgacgacagc
     6781 atgccaacgc caaccgcaga aactatggca atattttctt tatagtcaat gcctgaggca
     6841 cccagctttt ctttcagtga ttcacaaatc tttaatgctt tgtccagatc attttttaac
     6901 acagtaaagg atatggatgc tttgccatcc ttacctgttg actgaacaat catattaacg
     6961 tagactttct gctttcctaa ttcaccaaac aacagtgaag caacacccgg cctgtcgggt
     7021 atatcacgta tggttatctt tgcttcatca tcttttgacg ttacgccgct aattacaaat
     7081 ttttccatca tctcctcccg tggcattact attgttcctt cattatcatt aaaacttgat
     7141 ctaacatgta atcgtatact atacttttta gcaaattcca ccgatctgga atgtaaaacc
     7201 ccggcaccaa gccgggctaa ttccagcatc tcttcataac tgatttcatt caatttctta
     7261 gtattggaaa caatccgtgg atcagcagta aacacaccat ccacatcggt ataaatttca
     7321 caatcgcgtg ttcccagcac tgttgcaagt gctactgctg aagtgtccga gccacccctt
     7381 c
//
LOCUS       DAOSZP010000024         7339 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_8609_length_7339_cov_3.474602, whole genome shotgun sequence.
ACCESSION   DAOSZP010000024 DAOSZP010000000
VERSION     DAOSZP010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 7339)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7339)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7339
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8609_length_7339_cov_3.474602"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..457
                     /locus_tag="PK348_01075"
     CDS             <1..457
                     /locus_tag="PK348_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK64835.1"
                     /translation="KERIVLLKSSIEAQIYLIDKIYDKIEERKKKRAKWVVENIGYQL
                     HNLYCAFEDLFKIIAREFENNIDNGSKYHYELLKRMAIAIEGIRPKLISEDTFPLLDN
                     LRSFRHFFRHAYSYDLDKRKIDIVLEDALKLKELYKHDIERFLKELESE"
     gene            595..1131
                     /locus_tag="PK348_01080"
     CDS             595..1131
                     /locus_tag="PK348_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018052634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HXK64836.1"
                     /translation="MLDALITSKTRLKILLKFFINPSTKGYLRELAAQMNESTNAVRV
                     ELDRLVEAGYLERNANGNKVYYNANTGHPLFKDIHNVVKKYLGIDVLIDSVLAKLGKI
                     HEAYIVGDYANGVDSGIIDVVIVGTVDYSYLAQLIEKAEGIIKRKIRPLVLSPKEKTK
                     YKEKLNLDKALLVWHNGK"
     gene            1121..1621
                     /locus_tag="PK348_01085"
     CDS             1121..1621
                     /locus_tag="PK348_01085"
                     /inference="COORDINATES: protein motif:HMM:NF033644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UpxY family transcription antiterminator"
                     /protein_id="HXK64837.1"
                     /translation="MESNWYAVYVRSRHEFKVHNALLAKGIESFLPVVERLRQWKDRK
                     KLIPFPLFPGYLFVKLPDDKVYFLHVLKINGVVKILGDSCGYQPVAEDQIVSIYKMVN
                     SKLAIDEHPYLKEGQKVKIVTGPLSGVQGILQRKGGEQYVVVSIDILQKGVAVKVNIQ
                     SIEPVI"
     gene            1698..2099
                     /locus_tag="PK348_01090"
     CDS             1698..2099
                     /locus_tag="PK348_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2177 family protein"
                     /protein_id="HXK64838.1"
                     /translation="MNYIKMYVTALILFLIIDALWLTVIAKNMYKQYLSYLMGDVVWW
                     AVIIFYVLFIIGLQYFCIIPAINAQSAMHAFYAGALFGLVSYATYDLTNQATIKGWPV
                     LITCIDLLWGAFIGATVSALTTKIALLLKWH"
     gene            complement(2145..2714)
                     /locus_tag="PK348_01095"
     CDS             complement(2145..2714)
                     /locus_tag="PK348_01095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK64839.1"
                     /translation="MKKNILALLFTLCITAIPSLTFAIADIAVIGGYTPYGNIEFYEE
                     RYDSLKGYNYGVMGHLNVDTEVIMFGFGFYFHGEQLHYTFNGDTRDFTVKQSWGPDII
                     IMITASEIIAPYVRAGIALLSELKYDYGYDKKEKLRFLNSGWWAIGSGIHITEQVLVF
                     GELQRLSMSHDNEHSSKRYTVNAGAMFNF"
     gene            2974..4122
                     /locus_tag="PK348_01100"
     CDS             2974..4122
                     /locus_tag="PK348_01100"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012673735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HXK64840.1"
                     /translation="MNIPFHVPYIDDSDLEAAVSAIKSGWTTMGPKTIEFEESFAQYT
                     GMPFSVAVSSCTAALHLALKVCNIGPGDEVIVPVNTFVATAEVVCYCGATPVFADIDR
                     DSHCISVDSLYRLLSPKTRAIIPVHFAGTPCEMDVIDDIARQHNLYVIEDAAHALPSW
                     YKNRLAGTIGHVGCYSFYATKTLATGEGGMLVTHNEEWARRAGRLRLHGMNRDAWRRY
                     DRGGSWQYDIEEMGYKYNMTDIAAAMGLSQLKKVEMLQKKREAIAQRYNDAFSENDAL
                     IPYVVNKGISSWHLYPLKINLEALTISRDEFIVKLEECGIKTSVHFIPLYRFSYFVKR
                     YGNQESMFPDSEWVFAREISLPIFPSMTDEQIDYVIDCVLSIAKQFKR"
     gene            4125..5945
                     /locus_tag="PK348_01105"
     CDS             4125..5945
                     /locus_tag="PK348_01105"
                     /inference="COORDINATES: protein motif:HMM:NF014740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HXK64841.1"
                     /translation="MKKVLYTIKPLAVVFFVADMLCIIAGYGIASLLFSYSLDAFITP
                     AVLHAFLTIAGLYITQSYRILWRYATMQDYYRIIEGFTGATVLLLIYSSVVNLVSNRV
                     LFLASAGGLALLMVYRVIIRETKPQKIALPKKKSKIILIAGAGEAGRTLLAEFKRDGR
                     GDSIIGFVDDDTRKVGTLLCGKPVLGTTYDIPSLVEQYGVKECIVAMPSAPQKEIDRV
                     IKIIRSSGNVSISILPALTKLFEKALTPELRGIGVEELIGREEIEIDSAAIEAYCKGK
                     TVLITGAGGSIGSEICRQLLHFKAGKIIAVGRGEHSIYNLKNMLDDYAKYFEKPEIYY
                     YIADINDAVRMQKILTKHKPHIVFHAAAHKYVDIMEQNPQEALRNNCLGTRTVLEVAR
                     AAGVGQFVCISTDKAVRPVSVMGATKRMAEIVTLAQNSPDMRTCCVRFGNVIGSRGSV
                     IPLFYQQIAKGGPVTITHPDMTRYFMSIPEAVMLVIHAAMLSNGGEIFVLDMGKQYKV
                     EEVARNLITLLGYEPDVDIPVVYTGIRPGEKLQEDLWNHGEELVPTLHKKIYKIAHNG
                     FDALSVTKIKELTPDYVQSLHEEECKQLIQAIVPDYRPFN"
     gene            complement(5938..7011)
                     /gene="mnmA"
                     /locus_tag="PK348_01110"
     CDS             complement(5938..7011)
                     /gene="mnmA"
                     /locus_tag="PK348_01110"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HXK64842.1"
                     /translation="MKIAVAMSGGVDSSVTAALLVEQGHQVIGLTAKILLCAEMDGCQ
                     PRYDVCCSPESIHDAKKVATQLRIPHYIVNVEDEFSQNVIDPFCAEYIKGRTPSPCIN
                     CNTSIKFPELLNFAKELGFDMLATGHYATICKSNRYYIKRGVDTDKDQSYFLFNLSQD
                     VLKHLMLPLGQSTKREIREKARELGLATAHRPESQEVCFVQDNDYPRFIEQRLGFCPP
                     HGDIVTTDGKVLGRHKGIHHYTIGQRRGLGIAWEHPLYVVAIDAIHNRIIVGSKDELL
                     AKGLLATQVNFMKVTIAHNTPVWVKTRSTQKPFKAHATYENDILTVTFETPQASITPG
                     QAVVCYDDDGAILFGGWIDKSIN"
     gene            complement(7159..>7339)
                     /locus_tag="PK348_01115"
     CDS             complement(7159..>7339)
                     /locus_tag="PK348_01115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK64843.1"
                     /translation="INDYEKAKEIVETIKSMQSLYTVDVGKYKMNQNNIIIIDRQCVA
                     MLKRMMKKNIAAGKI"
BASE COUNT         2244 a         1288 c         1556 g         2251 t
ORIGIN      
        1 aaaagaaaga atagtgttgc tgaaatcgtc aatagaagct caaatatatt tgatagataa
       61 aatctatgac aaaattgaag aaagaaaaaa aaagagagcc aagtgggttg ttgaaaatat
      121 tggctatcag ttacacaatt tgtattgtgc ttttgaagat ttatttaaaa taattgcacg
      181 ggaatttgaa aataatattg ataatggtag caaatatcat tatgaattgc tgaagagaat
      241 ggccattgca atagaaggta tacgacctaa attaatatct gaagatacat tccccttgct
      301 tgataattta cgctcatttc gtcatttttt tagacatgca tattcctatg atttagataa
      361 gcgtaaaatt gatattgttt tagaagatgc attaaaacta aaagaactct ataaacacga
      421 catagagaga tttttgaaag aattagaatc ggagtaatat tcatgaaatg aaatatattg
      481 ctcaaagtaa aaagttgaac aaaatgtaag aatgtgcaaa tttcttcttc ttttagtaat
      541 gggtgtttat taataacttg acatgaatga acaaatttat tactcgtttt gaatatgtta
      601 gatgcactga taacatcaaa aacgcggcta aagattttac tcaaattttt tattaatcct
      661 tccactaaag gatatctgcg tgaattagcc gctcagatga atgaatccac caatgcggtc
      721 cgtgtggaac ttgatcgcct tgttgaggcg ggctatttag aacgcaacgc caatggcaat
      781 aaggtgtact ataatgcgaa tactggccat ccccttttta aagatattca caatgttgtg
      841 aaaaaatatt taggtattga tgtgttgatt gacagtgtgc ttgccaagtt agggaaaatt
      901 catgaagcat atatagtagg tgattatgcc aatggtgttg atagtggtat tattgacgtt
      961 gtcattgtag gcaccgttga ttattcttac ctggcacagc tcattgaaaa agctgaaggt
     1021 attataaagc gaaagatacg gccgctggta ctatcaccaa aggaaaaaac aaaatataaa
     1081 gaaaaactca atttagacaa agcgctactg gtatggcaca atggaaagta actggtatgc
     1141 ggtatatgtt cgctctcgcc atgagtttaa agtgcacaat gctctgttag caaaaggcat
     1201 tgagtcgttt ttgccggttg ttgaaagatt gcgtcaatgg aaagacagaa aaaagctcat
     1261 tccttttcct ctgtttccag gatatctgtt tgttaagctc ccggatgata aagtttattt
     1321 tttgcacgtt ttaaaaatta atggtgtggt aaaaatattg ggcgatagct gtggatatca
     1381 gcctgttgct gaagatcaga tagtgagtat atataaaatg gttaatagca agcttgccat
     1441 agatgagcat ccatatttaa aagaaggaca aaaggtaaaa atagtcactg gtcctctttc
     1501 aggtgtacag gggattctgc aaagaaaagg gggagaacaa tatgttgttg tgtcaattga
     1561 cattttacag aaaggagtag cagtaaaagt taatatacag tcaattgagc ctgttatatg
     1621 acttttttca tgaaaaaatg attgttttat ttctacaatt gaagtataaa aattatactt
     1681 taaatggagg ttctatcatg aattacataa aaatgtatgt tactgcatta attctttttc
     1741 tgataatcga tgcactatgg ttaactgtta tagcaaaaaa tatgtacaaa caatatttaa
     1801 gctatctcat gggtgatgtg gtgtggtggg ctgttattat attttatgtt ctatttatta
     1861 ttgggttaca atatttttgt attatacctg caattaacgc acaatcagca atgcatgcct
     1921 tttatgcagg agcattattt gggcttgtaa gctatgcaac gtatgacctg acaaaccagg
     1981 caaccattaa aggatggcca gtgctcatta catgtattga tttgttgtgg ggtgcgttta
     2041 taggagctac tgtcagtgca cttacaacaa aaattgcatt acttttgaag tggcactaaa
     2101 acgttgaaac aaaacacacg tttttctggt ttttataacg aatgttaaaa attaaacatt
     2161 gctccagcat ttactgtata acgtttagat gaatgttcat tgtcatgcga catggacagt
     2221 cgttgtaatt cgccaaaaac gagaacctgt tctgttatgt gaatacctga gcctatggcc
     2281 caccagccac tatttaaaaa tctgagtttt tcttttttat cgtatccata atcgtatttt
     2341 aattcactca aaagtgcaat gccagctctt acataaggag caataatctc acttgctgtt
     2401 atcatgataa taatatcagg cccccatgat tgtttaacag taaaatccct tgtatccccg
     2461 ttgaatgtat aatgcaactg ttcaccatgg aaataaaatc caaatccaaa catgataact
     2521 tcagtatcaa cattgagatg ccccatcact ccgtaattat accctttgag cgaatcatac
     2581 cgctcttcat agaactctat atttccgtag ggagtatatc ctccaataac tgcaatatct
     2641 gctattgcaa aggtcagtga gggaatagcg gtaatgcata atgtgaataa gagtgcaaga
     2701 atgttttttt tcatgactat cctcattatt ctgtgtaatg aattgtcagg aaccataggt
     2761 attgtacgtt ttaaaataat ttcatgatga actgataagc catctcacct ctataacggt
     2821 gaaagtatat ataaaaagta tatataattt ggtattcttt taatgaagtt aagtttatga
     2881 atatgtaata aaatttttca attctttttt catatttaac ctgctatttt cttccgaaaa
     2941 tatacatagt ctggtataca aggattgcgt gcaatgaata tcccgtttca tgttccctac
     3001 atcgatgaca gcgaccttga ggctgcagtc agcgccatta aaagtggctg gacaacaatg
     3061 ggtccaaaaa caattgagtt tgaggaaagc tttgcccagt atacaggaat gccattttct
     3121 gttgcagtaa gttcgtgtac ggctgcgctg catttggcgc taaaagtatg caacattggc
     3181 ccgggtgatg aggtcatagt tcctgttaat acctttgttg ccacagcaga ggttgtttgt
     3241 tactgtgggg ctactccggt gtttgctgat attgacaggg actcccattg catcagtgtt
     3301 gattcactgt accggttact atcgcccaaa accagagcca ttatcccggt acattttgca
     3361 ggaactccct gcgaaatgga tgttatagat gatattgctc gtcagcacaa cctgtatgtc
     3421 attgaggatg cggcgcatgc attaccatcc tggtacaaaa atagacttgc tggtacaatt
     3481 gggcatgttg gctgttacag cttttatgca accaagacgc ttgcaacggg tgaaggcggg
     3541 atgctggtaa cgcacaatga agagtgggcc aggcgggccg ggagattgcg actgcacggc
     3601 atgaaccgtg acgcatggag gcgctacgac aggggtggaa gctggcagta tgacatcgaa
     3661 gagatgggct ataaatataa catgaccgat attgctgcgg caatgggtct aagtcagctt
     3721 aaaaaagtgg aaatgttgca aaaaaaacgg gaagcgatag ctcaaagata taatgatgcg
     3781 ttttctgaaa atgatgcatt aattccgtat gttgtcaaca aaggaatttc ttcatggcat
     3841 ctgtatccgc tcaagataaa tcttgaggcg ctaactattt cacgcgatga atttattgtg
     3901 aaattggaag aatgcggcat aaaaacaagt gtgcatttta ttccgctcta ccgattttct
     3961 tattttgtaa aacgatatgg caatcaggaa tcgatgtttc cagatagcga gtgggtgttt
     4021 gcacgggaga ttagtcttcc aattttccct tcaatgacag atgaacaaat tgattatgtg
     4081 attgattgcg tgctgtcaat tgcaaaacaa ttcaaaaggt aattatgaag aaggtactct
     4141 atactataaa accattggct gttgtctttt ttgttgctga tatgctgtgt attatcgctg
     4201 gatatggtat tgcatcgcta ttgttttcat attcattgga tgctttcatt acacctgccg
     4261 tattgcatgc atttcttact atagcaggat tgtatattac acagagctat cgcatattat
     4321 ggcggtatgc cacgatgcag gattattatc gtatcattga agggtttaca ggcgctacag
     4381 tgctattgtt gatatacagc agtgttgtga atcttgtcag taatagagtt ctttttcttg
     4441 ccagtgcagg tggccttgca ttactcatgg tctatcgtgt cattatacgg gaaacaaagc
     4501 ctcagaagat cgcattgcca aaaaagaaat caaaaattat tttaatagct ggggcaggtg
     4561 aagcaggaag aaccttgctt gctgaattta aacgtgatgg cagaggtgat agtatcatag
     4621 gatttgttga tgatgatacg cgcaaggttg gtacattgtt atgtggaaag cctgtgctgg
     4681 ggaccactta tgatatacct tccctggtag aacagtatgg tgttaaagag tgcattgtgg
     4741 caatgccatc tgctccgcaa aaggagattg atcgagtcat aaagattatt cgctcaagtg
     4801 gtaatgtcag catttccatt ttaccagcgc taacaaagct ttttgaaaag gctctgaccc
     4861 cggagctacg aggaataggc gttgaggaac tcattggtcg tgaagaaatt gagatagata
     4921 gtgcggcaat tgaagcatat tgtaagggta aaactgtgct tatcactgga gctggcggaa
     4981 gcataggttc tgaaatttgc aggcagctat tacacttcaa agcagggaaa atcattgcag
     5041 ttggcagagg cgagcattca atatataatc taaaaaatat gcttgatgat tatgctaagt
     5101 actttgaaaa acctgaaatt tattactaca tagctgatat taatgacgct gtgcgcatgc
     5161 aaaaaatttt aaccaaacac aaaccacata ttgtattcca tgctgctgcg cacaaatatg
     5221 tcgatatcat ggagcaaaat ccgcaggaag cactgcgcaa taactgcctg ggtacacgca
     5281 cggtactgga ggtagcacgc gctgcaggtg ttggtcagtt tgtctgcata tcaacggata
     5341 aggcggtgcg ccccgtaagt gtgatgggcg caaccaagcg catggccgag attgtaacgt
     5401 tagcgcaaaa cagccccgac atgcgcacct gctgtgtgcg gtttggcaat gttattggca
     5461 gccgtggttc ggttatacct ctgttttacc agcagattgc aaagggtggg cctgtgacca
     5521 ttacccatcc agatatgacg cgctacttta tgagcatacc tgaagccgtg atgcttgtta
     5581 ttcatgctgc catgctttca aacggtggcg aaatatttgt gcttgatatg ggaaaacagt
     5641 ataaggtgga agaggtagca cgcaatttga taacgctttt agggtatgag cctgatgtcg
     5701 atattcctgt tgtatatact ggcattcgac caggggaaaa actgcaggaa gatttatgga
     5761 atcatggcga ggagcttgtg ccaacccttc ataaaaaaat atataaaatt gcccacaatg
     5821 gattcgatgc gctatcggtt actaaaataa aagaactaac acctgactac gtgcagagct
     5881 tacatgaaga agaatgcaaa caattaatac aagcgatagt tcctgattac aggccattta
     5941 attaatactt ttatctatcc agccaccaaa tagtattgca ccgtcatcat cgtagcatac
     6001 aacagcctgg ccgggggtga tgcttgcctg aggtgtttca aaggttacgg taagaatatc
     6061 gttttcatac gtggcatgtg ctttgaacgg tttttgtgtt gagcgtgttt ttacccatac
     6121 gggagtatta tgggcgatag ttactttcat aaagtttacc tgtgtggcaa gaagcccctt
     6181 tgccagaagt tcatccttgc ttcccacaat gatgcgatta tgtatagcat caatagcaac
     6241 aacgtataac gggtgttccc aggcaatgcc aagcccacga cgctgcccga tggtgtagtg
     6301 atgaattcct ttatgcctgc ctaaaacttt gccatcagtt gtgacaatat cgccatgagg
     6361 aggacaaaag cccaggcgtt gttcaataaa gcgtggataa tcattatcct gaacaaaaca
     6421 tacttcctgg ctttcaggcc tgtgggcagt tgccagtccc agttcacgtg ctttttcgcg
     6481 tatctcacgt tttgtggatt gaccaagtgg aagcataagg tgctttaaca catcctgcga
     6541 aaggttaaac aaaaaatatg actgatcctt atcagtatca acgccacgct tgatataata
     6601 gcggttactt ttacatatag ttgcataatg gcccgtagca agcatatcaa atcctaactc
     6661 tttggcaaaa tttaaaagct ctggaaattt tatgctggtg ttacagttaa tgcaggggct
     6721 gggtgtgcgt cctttaatat attcagcgca aaatggatct ataacgtttt gtgaaaattc
     6781 atcttcaacg ttgacaatat aatggggaat acgtaactgt gtggcaactt tttttgcatc
     6841 atgtatgctt tcgggactac agcatacatc ataccgtggc tggcagccat ccatttcagc
     6901 acacagcaaa attttagcgg taaggccaat cacctgatgc ccctgttcaa caagtaatgc
     6961 agcggtaact gatgaatcaa caccaccact cattgctact gctattttca taggtattcc
     7021 tgttaatatt aatgattact agttagtgat aatatacctg aaaagaggcc aaacagtaaa
     7081 aaatatttta tcaatgggga aaatttttat caggattaac tactgcgctt ttttgcggca
     7141 gaaaagctta taatatcttt atatcttacc tgcagcaata ttttttttca tcatcctttt
     7201 gagcattgct acacactggc gatcaatgat gataatatta ttctggttca ttttgtattt
     7261 gccaacatca actgtataaa gagattgcat gctttttata gtttcaacaa tctcttttgc
     7321 tttttcataa tcgtttatt
//
LOCUS       DAOSZP010000025         7248 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_8740_length_7248_cov_4.374253, whole genome shotgun sequence.
ACCESSION   DAOSZP010000025 DAOSZP010000000
VERSION     DAOSZP010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 7248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7248
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8740_length_7248_cov_4.374253"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1310
                     /locus_tag="PK348_01120"
     CDS             <1..1310
                     /locus_tag="PK348_01120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK64844.1"
                     /translation="SGTVHYLYDHLNDDYLPITSGAFAGTFVAGACGCYHPWSVTFTA
                     TGGSTTTANVAAGTSNISGLVVGAEIEFLSGTAANLGQRRTITAIEIAAGAGTTIITF
                     NAAPGAVVNTDTFRISSGSFFVLATGTLASGSFKRWDIATAAWSNLSITTLPATWGTD
                     GRMVTPAILTDFYDSGTVASSTGTSLTDVTKAWTADQWINSQVRITGGTGIGQIRRIT
                     DNDGTVLTVASAWTTNPDATSTYKIEGDENAIYVLGNNAVTMYKYSISANTWAVVAPT
                     TARAAAPIAGMSADFVGKTGDAVWADITDIKDGRYIYSLRGGTAVLDRFDIAGGTAGA
                     GAWTAVTYSPALQTFATGDSTFWDYENIIIAKEGTAAIPQRFYKYSVVDNTIVPFTSD
                     WYFGGAALLGNKIWVKKLSSAGLVRWVYCLQSTSTNLRRIMIF"
     gene            1315..1482
                     /locus_tag="PK348_01125"
     CDS             1315..1482
                     /locus_tag="PK348_01125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK64845.1"
                     /translation="MLLTILANNVMLTATPPAQTGGGSGVAFFKRKEFKKLKENDKRM
                     MKIFEAFLKEV"
     gene            1750..2490
                     /locus_tag="PK348_01130"
     CDS             1750..2490
                     /locus_tag="PK348_01130"
                     /inference="COORDINATES: protein motif:HMM:NF016471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HXK64846.1"
                     /translation="MEKKYIYKDSLVSDSLRLQIKDVDGKKGIVTGYFSDFNSIDSDG
                     DIIKPGAFQKSISQNGPQSSKPRIKHLLNHDSSKPLGVLEVLKEDTKGLYYESRLGTH
                     SLGVDFIKMVDSGLISEHSIGFQTVKYNQLKPWNEWKQGEAARELTELKLYEGSSLTA
                     WGANMNTPLTGLKTEQKVRKINDRIDILIKSLRDGTFSDETFDLLEIELKQMQQAMID
                     LTTEPEQTTQPDEEKAVADIKQFLKTLN"
     gene            2497..3762
                     /locus_tag="PK348_01135"
     CDS             2497..3762
                     /locus_tag="PK348_01135"
                     /inference="COORDINATES: protein motif:HMM:TIGR01554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HXK64847.1"
                     /translation="MEKTLQEQLVELKSQLETSLTEKAKSEIALQIKSLEEKMKPVDL
                     TEIKAEIKKVTDWQVTKDEADKKNQQALDELIAHQSKRKGPETGTSFKDALGEAMEQK
                     KADIQGYTKNRQQVSLELKAVGNMGSGNLTTSGTETFAGNTMIGGVGRKPYEISHIRN
                     VVNVQPIATDSAYVIRDNAGEGGPTAVSMAAAKPQSDRDYVKLIVPVTKVAHYFKIPE
                     EMLADNAWLQNEITAIGLEELLAKEDDLLLNQVAGAGLFGGLTTATNSTAFAAPASLA
                     LGVDLANNYDVLVAAWTQARNAKVSPNLVLCNPSDYAKMILTKESATSGAYVFGAPNI
                     AIPNIFGIPLVPHTAMTSDKFLIGDFSKVTLGQRAGFSVRFYDQNEDDAIKNMVTVVI
                     EERITIVAGRADYLYYGDFSDGRAALETA"
     gene            3814..4386
                     /locus_tag="PK348_01140"
     CDS             3814..4386
                     /locus_tag="PK348_01140"
                     /inference="COORDINATES: protein motif:HMM:TIGR02215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK64848.1"
                     /translation="MNANNIISIQDVTVGAITEPITVQDVKDYLRIEGFTAVGGTEVA
                     FNDDDIIIGIIITAAREQFEKYSGITLTANRSKKVVLNNFYGALELPFGPVKEVTALI
                     NSNGDDLLSSVETVGTMWVCLTSPTGENIAVTYTCGYGTTGIESLPASIKLDLLRACA
                     YFYTNRGDAADVNNFISQLAKKYSRDVWLV"
     gene            4696..5253
                     /locus_tag="PK348_01145"
     CDS             4696..5253
                     /locus_tag="PK348_01145"
                     /inference="COORDINATES: protein motif:HMM:NF016754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HXK64849.1"
                     /translation="MIGFDSYARKLAAAPEEIVKEIEQEVIFAAEAFAGYAKKDVRAQ
                     SFDNGQLAGGIQTKKISSLTYDVTSTAKHSPYVEFGTKRKFRAQADVDSSIYNGKGGG
                     TMAQFIEAIKGWVKRKGIRAGTYSIKTRRRTGSKSKKASEDNALVMGIVFAILRNGVK
                     ARPFFFKQRARVKKEFIDRLNALLK"
     gene            5250..5678
                     /locus_tag="PK348_01150"
     CDS             5250..5678
                     /locus_tag="PK348_01150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK64850.1"
                     /translation="MRNVSTKLLNSYFALLNGTVSVPVYIDSVPDDEDGNYIELRVES
                     ESEVDRNDKSWLKEVIVIADIVTVFDTMVNPDIANGIDNEIGVLLCTTPFSGHNLTAQ
                     TGMQINRLQIDDATYLQEDDGTKKIYRKITRYNNLITIKI"
     gene            5806..6108
                     /locus_tag="PK348_01155"
     CDS             5806..6108
                     /locus_tag="PK348_01155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK64851.1"
                     /translation="MSTEESDCGVFKSFGPSNYKLNFSGLVDTAPDASEGSWTELLAL
                     FEANTPILSKFATVGNVPYITGTSRISSLNLAASSPSDTVKFTATVEFYGAVDITP"
     gene            6110..6487
                     /locus_tag="PK348_01160"
     CDS             6110..6487
                     /locus_tag="PK348_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK64852.1"
                     /translation="MNGAIKVEIDGNVIGLKFAYPAIRMFLDEMAAKSKFYYSQSDGG
                     DPMMTVEGIAKFIQCGYKNDCMIKEVDEVFNYEFFYNWVEQMNNDASIAEAVKKIFEC
                     YGNTEIKRVIDNAVKTPVDEKKN"
     gene            6559..6807
                     /locus_tag="PK348_01165"
     CDS             6559..6807
                     /locus_tag="PK348_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK64853.1"
                     /translation="MKDLYLIMEGRNRKMAAEQMTARNVAAIIGDVFTQVMSGQSSRE
                     MIFKMWPIAGEVVEEEKVMTREERRALLLRHNKIINKA"
     gene            6921..>7248
                     /locus_tag="PK348_01170"
     CDS             6921..>7248
                     /locus_tag="PK348_01170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK64854.1"
                     /translation="MTRSANTATESLKKLKPGSDQATNSLINLSRVAQDAPYGFIGIA
                     NNLNPLLESLQRLKASTGSTGSALTALKGALLGPAGLGLALGVVSSLVVSFGDKLFGA
                     KASMNSY"
BASE COUNT         2366 a         1206 c         1784 g         1892 t
ORIGIN      
        1 tatctggtac ggttcattat ttgtacgacc atttaaacga tgattattta ccaataacat
       61 caggtgcttt tgccggaaca tttgtagccg gggcttgcgg ttgttatcac ccgtggagtg
      121 ttacgtttac cgcaacaggt ggaagtacaa caacggcgaa tgtggcggcg ggaacttcaa
      181 atatcagtgg attagttgtt ggtgctgaaa tagaattttt atccggtacg gctgctaatt
      241 tagggcaacg cagaacgata acagcgattg aaattgccgc cggtgcagga acaacgataa
      301 taacatttaa tgctgctccc ggagcagttg ttaatactga tactttcagg atttcatcag
      361 gtagcttctt tgttttggct accggaactt tggcatcggg ttcttttaaa cgttgggata
      421 ttgccaccgc tgcatggagt aatttatcaa taactacatt acctgcaacg tggggaacag
      481 atggaagaat ggttacaccg gctatattaa ctgatttcta tgattcagga acggtagcgt
      541 ccagtacagg aacaagctta accgacgtta caaaagcatg gacggctgac caatggatta
      601 actcacaagt acgaataacc ggtggaacag gtattgggca aataagaagg ataacagata
      661 atgatgggac tgtattaacc gttgcttcgg catggacaac aaacccggat gcaacaagca
      721 cttataagat tgaaggggat gaaaatgcca tatatgtttt agggaacaat gccgtaacga
      781 tgtataagta ttcaatatcg gctaatacat gggcagttgt tgcacctacc acagcaagag
      841 cagccgcacc aatagcaggt atgagtgcag actttgtagg taaaacaggc gatgcagttt
      901 gggctgatat taccgacata aaagacggga gatacattta ttcattacga ggcggtacgg
      961 cggttcttga cagatttgat attgcaggcg gtacggcagg agcaggggcg tggacagccg
     1021 ttacttattc acctgcattg caaacattcg caacaggtga cagcactttt tgggattacg
     1081 aaaatatcat cattgccaaa gaaggaacgg cagcaatccc gcaaaggttt tataaatatt
     1141 cagtagtgga taacacgatt gttcctttta cttctgattg gtatttcgga ggtgcagctt
     1201 tgttagggaa taagatttgg gttaagaaat tatccagtgc tggcttggta agatgggtgt
     1261 attgcttgca atcaacatca acgaatttaa gacgaataat gattttctaa atgtatgtta
     1321 ttaaccattt tagcaaacaa tgtgatgtta acagcaacgc caccggcaca aactggcggc
     1381 gggagtgggg ttgcattttt taaacgaaag gagtttaaga agttgaagga aaacgacaaa
     1441 cgcatgatga aaatatttga agcattttta aaagaagtat aacatggaaa agaaaattgg
     1501 catcaaggcg aggcggcaag agaattgact gaattaaaat tgtacgaggg ttcatcatta
     1561 acggcttggg gtgctaatat gtacacgccc ttaacgggaa tgaaagcata tgataaaata
     1621 aataaattta ataatcgtat tgatataatg attaaggcaa tgcgaagtgg taattttacg
     1681 gatgaaacat tccagatgtt ggagattgaa ttgagacaac ttcagcaaac tttttaaacg
     1741 aagtataaca tggaaaagaa atacatatac aaggattctt tagtcagcga ttcacttcgc
     1801 ctgcagatta aagatgttga cggtaaaaaa ggaatcgtta ccggatattt ttctgacttc
     1861 aatagtattg attcagatgg tgatattatt aagccgggag ctttccaaaa gagtatttca
     1921 cagaacgggc cgcaatccag caagccccgg ataaaacatt tgcttaacca tgattccagt
     1981 aagccattgg gagtgcttga agtgctgaaa gaagatacta aagggcttta ttacgaaagc
     2041 aggttaggca ctcattcttt aggtgttgac tttattaaaa tggtggacag cggattaata
     2101 agcgaacact caataggatt tcaaacagta aaatacaatc agttgaagcc ctggaatgaa
     2161 tggaagcaag gcgaagcagc aagggaattg actgaattga aactttatga aggaagctct
     2221 ttaactgctt ggggtgccaa tatgaacact cctttaacag ggttgaaaac tgagcagaaa
     2281 gtgaggaaga ttaatgacag gatagatatt ttgataaagt cattaaggga cgggacattt
     2341 tcagatgaaa catttgattt acttgaaata gaattaaagc agatgcagca ggcaatgata
     2401 gacctgacca ctgagccgga acaaaccact cagccggatg aagaaaaagc agttgccgat
     2461 attaaacaat ttttaaaaac gctaaattaa aatagcatgg aaaagacatt gcaagaacag
     2521 ttggtagagc ttaaatcaca gcttgaaacc agcttaacag aaaaagcaaa atcagagatt
     2581 gctttacaaa taaaatctct ggaagaaaaa atgaagccgg ttgatttgac agaaatcaaa
     2641 gcggaaatca aaaaagttac tgattggcag gtaaccaaag acgaagccga taaaaagaat
     2701 cagcaggctt tggatgaact gattgctcac caatcaaaaa ggaaaggacc ggaaactggt
     2761 acttctttta aagatgcttt gggcgaagca atggagcaaa agaaagcaga tatccagggt
     2821 tatacaaaga accgtcagca ggtaagcctt gaactaaaag cggttggtaa catgggttct
     2881 ggaaacctga caacttcagg cacagaaact tttgccggca ataccatgat tggcggtgtg
     2941 ggccgaaagc cttacgaaat cagccacatc cgtaatgtgg taaatgtgca gccgattgct
     3001 actgattcag cttacgttat tcgtgataac gcaggcgaag gcgggcctac tgctgtttct
     3061 atggctgctg caaagcctca atcagaccgt gactatgtga agctgattgt acctgtaacc
     3121 aaagtggcgc attacttcaa aattcctgaa gaaatgctgg ctgacaatgc ttggttacag
     3181 aatgaaatta cagcgattgg ccttgaagaa ttgctggcaa aagaagatga cctgttgtta
     3241 aatcaggttg ctggtgcagg tttgtttggc ggtttaacaa ctgcaacaaa tagcacagcg
     3301 tttgctgctc cggcttcttt agctcttgga gttgacctgg ccaataatta cgatgtactt
     3361 gttgccgcat ggacacaggc aaggaacgca aaagttagcc ctaacctggt tctttgcaat
     3421 ccttcggatt atgcaaaaat gattcttaca aaagaatctg caacaagcgg agcatatgta
     3481 tttggtgcgc ctaacattgc cattcctaat atctttggta ttcctttggt gccacatact
     3541 gcgatgacat cagacaaatt tctgattggc gatttcagta aagtgacact tggacaaagg
     3601 gctggctttt ctgttcgttt ttacgatcag aacgaagatg atgctattaa aaacatggta
     3661 accgttgtta ttgaagaaag gataaccatt gttgcaggtc gtgcagacta cctgtattat
     3721 ggtgatttct ctgacggacg tgcagcactg gaaacagcat aagcataatg gttgcagggc
     3781 gggttcgatt cccgcccatg cttcaaaaaa aatatgaacg caaataatat catatcaatc
     3841 caagatgtaa cggttggtgc cattaccgag ccgataacag tgcaagatgt aaaagactat
     3901 ttgcgtattg aaggctttac ggcagtaggc ggcacagagg tagcgttcaa tgatgatgat
     3961 attattatcg gaataattat aacggcagcg agggagcagt ttgagaaata tagcggcatt
     4021 actttaacgg caaatagaag taagaaagta gtgctgaata atttttatgg tgcgcttgag
     4081 ttaccattcg ggccggttaa agaagtaacg gctttaatta attctaacgg agacgacttg
     4141 ctgtcgtccg ttgaaacagt cggcacaatg tgggtatgtc ttacatcccc aacgggcgag
     4201 aacatagctg ttacctacac ttgtggttat ggcactaccg gtattgagtc gctacctgca
     4261 tcaattaaat tagacttgtt acgtgcttgc gcttactttt atactaatag aggtgatgcg
     4321 gcagacgtga ataatttcat ttcacagctt gcgaaaaaat atagtagaga tgtatggttg
     4381 gtgtaatgaa accgataaga ttaattaaga taacatcaac gcaagatgtt aatggacgat
     4441 gggtagaaac agaatcggct gcttataatg tttgggcttc actgttaaaa acaggaagca
     4501 gcaggtctgc gaataatgga cagacgagta taagcaacac gattcagttt aagatgtatg
     4561 ccaaaggatt tgacatatcg gtaaactggc gattagtgtg ggcagggcgg caatatacgg
     4621 tgaatggtat tgataggata gacgagaaat tgtttaatat attgataaca gcgacggcaa
     4681 atggtaagag ttaatttgat agggtttgat tcgtatgcaa ggaagctggc agcggcaccg
     4741 gaggagattg ttaaagagat cgagcaagag gtaatttttg cagccgaagc ttttgcaggg
     4801 tatgcaaaaa aggatgtaag ggcgcagtcg tttgacaatg gccagttggc cggaggtatt
     4861 cagacaaaaa aaataagttc tttaacatac gacgtaacaa gcacagcgaa acattcacct
     4921 tacgttgaat ttggcacaaa aagaaaattc agggcacagg ccgatgttga ttcatcaata
     4981 tacaatggta agggcggcgg tacgatggcg caatttatag aggcgataaa aggctgggtg
     5041 aagcgtaaag gcataagggc tggaacgtat tctataaaaa cgagaagaag gaccggaagt
     5101 aaaagtaaaa aagcgagtga agataatgct ttggtgatgg gtatagtatt tgcgattttg
     5161 cgtaatggcg taaaggcaag gccgtttttt ttcaagcaaa gggcgagagt gaagaaagaa
     5221 tttattgaca ggttaaatgc tttattgaaa tgagaaacgt ttcaacgaaa ttgctcaata
     5281 gttattttgc tctacttaat ggaacggtta gcgttcctgt ctatattgac agcgttcctg
     5341 atgatgaaga tggcaattat attgagttaa gagttgaaag cgagagcgaa gtagaccgaa
     5401 acgataaaag ctggttaaag gaagtgatag ttattgctga tattgttacg gtctttgata
     5461 caatggtaaa ccctgatatt gctaatggga tagataacga gataggcgtt ttactctgta
     5521 ctacgccatt ctccggtcat aatctcacag cacaaacagg tatgcagata aatagattgc
     5581 aaattgatga tgctacttat ttgcaggaag atgatgggac aaaaaaaatt tatagaaaaa
     5641 taacgagata caataattta ataacaatta aaatttaata caatggcaga cccaacaaca
     5701 gtaggcggta gtgaattaat atggtcgtat tcaacaaacc ttgttacacc gtcgtataaa
     5761 tcggtagtat gcaagcagtc ggcatcttta gatggttcac gaaatgtgag cactgaggag
     5821 agtgactgtg gtgtttttaa atcctttggc ccaagcaatt acaagttaaa tttttccgga
     5881 cttgttgata ctgcacctga tgcaagtgag ggcagttgga cggagttgct tgcgttgttt
     5941 gaggcgaaca ctcccatcct tagcaaattc gcaacggtgg gaaacgtacc ttacataacg
     6001 ggcacgagca gaatctcaag tttgaattta gccgcatcat ccccaagtga tacggtcaag
     6061 tttacggcaa cagttgaatt ttatggagca gttgatataa caccttaata tgaacggagc
     6121 aataaaagta gagatagacg gaaatgttat cggattaaaa tttgcttatc ctgcaataag
     6181 aatgtttttg gatgagatgg cagcgaagag taaattttac tactcgcagt cagacggcgg
     6241 ggatccgatg atgacagtgg aaggcattgc aaaatttatc caatgcggtt ataaaaatga
     6301 ctgcatgata aaggaagttg atgaggtgtt taactatgag tttttttata attgggtgga
     6361 gcaaatgaac aacgatgcaa gcattgccga agcggtgaaa aaaatatttg aatgttacgg
     6421 caataccgag ataaaaagag taatagacaa cgccgtaaaa actccggttg atgaaaaaaa
     6481 aaattagggt gggatgatat agaaagtata ttactctatg aacttgggta cagacgaaat
     6541 gagattaacg cccttcttat gaaagattta tatcttatta tggaagggcg taaccgtaaa
     6601 atggcggcag agcaaatgac ggcaagaaat gtggcggcga taattggcga cgtgtttacg
     6661 caggttatga gtgggcagag ttcaagagag atgattttta aaatgtggcc gatagcaggt
     6721 gaagtagtag aagaagaaaa agtaatgaca agagaggagc gacgggcgtt gttattgagg
     6781 cataataaaa taattaacaa ggcatgaacg atttatcagt cagaataggg gcggacgttg
     6841 cgccggcagt ggcagggctt caagaggtca gcgataggtt gcgcaatacg gctaagagcg
     6901 gcgaggcgtt cagtgcgtca ttgacacgaa gcgcaaatac ggcaacggaa tcgcttaaga
     6961 agttaaagcc gggctctgat caagcaacta actcgttaat aaatttatcg agagttgcac
     7021 aagatgcgcc ctatggattt attggcatag ccaacaattt aaatcccctg cttgaatcac
     7081 tccagagatt aaaggcatca acaggtagca ctggcagcgc attgactgca ttaaagggtg
     7141 cgttgcttgg ccccgcaggt ttagggcttg cgcttggcgt tgtatcttca ttggttgttt
     7201 cttttggtga taaattattc ggtgcaaagg ccagcatgaa cagttacc
//
LOCUS       DAOSZP010000026         7169 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_8856_length_7169_cov_3.169947, whole genome shotgun sequence.
ACCESSION   DAOSZP010000026 DAOSZP010000000
VERSION     DAOSZP010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 7169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7169
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8856_length_7169_cov_3.169947"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            53..652
                     /locus_tag="PK348_01175"
                     /pseudo
     CDS             53..652
                     /locus_tag="PK348_01175"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
     gene            747..3074
                     /locus_tag="PK348_01180"
     CDS             747..3074
                     /locus_tag="PK348_01180"
                     /inference="COORDINATES: protein motif:HMM:NF014900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HXK64855.1"
                     /translation="METLRKIVPHYDDFERVNTLRWRIINAPQEVCIERARYLTQSMM
                     HHWEKPALTRMSLALEHILNNISVIIRDDELIVGCRTSKLKGAPLFPENKSRWIEGDC
                     DNFDKRLLQKALITQKEKQELSANILPFWHGKTVEDIFESRLPQDVMEDMDKYIFTMM
                     LEITYGIGHFTMNYSKVLHLGLKGIISHLEQKMHELEVQGEANEKWLLYDAMIRSCKA
                     AIVFAKRYAQKAREMAGKTKRRKRAKELLEIARICEKVPEFPAETFHEAVQSLYFIHL
                     VTQIESGGNSVSIGRIDQILYPFYEKDMKSGRITQDKARELISLLFIKMNEIWNVLEE
                     AYIPGGEGAEGKTTQNVTIGGVDRQGNDVTNELSYIVLDAYADIRTVQPNFSVRISKK
                     TPKEFLIKAVEYARDGVLMHFFNDEIVIETLMKAGHSLEDARDYALVGCVEPNAQGKC
                     FGSTFAVQFSGIKCVEFALSNGVDNIFGYKSGIETGDPSTFHTFDDVWNAYDRQVKHF
                     MNQMARGMEILDKTIAEHVPSPFASVMIDGCIEKGKDLTSGGAVYNSTGVQLIGFANI
                     VDSLYGVKKAVYDQKYCSIAELAQWLSDDWQDVEDKRVYFFRKIPKFGNDNDEVDGLG
                     IKVLEHFCDTVSSHKNFRGGTFWPGVFVVGFHISFGAFTGATADGRHAGDVLGNGLTP
                     TTGNAISGPTAIMNSITKLPLTKIYNGANLNMRFNGNKISTEHLAYLIETYFTKGGMQ
                     VQFNMVDSKILRDAQKNPKNTGIYLFASAGIRLNL"
     gene            3169..4104
                     /locus_tag="PK348_01185"
     CDS             3169..4104
                     /locus_tag="PK348_01185"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HXK64856.1"
                     /translation="MSKIMTTQEAITRFVNDGDFLFIGGYICRPPFAAIHEIIRQKKK
                     DLTVVRSNTADDFDMLIGAGCVKRFICTFISLGLYGLARCFRRSIEKGIPHKIEIEEY
                     TNLSLPLMLMAGALGMPFVPVKDMVGTDLMKIKSFMGDNKYKFIDSPFDGSPVMLVPA
                     LNPDVAIIHVQQADEEGNAQMWGIGGDCKWGANAAKKVIVSCERIVSREVVGKDPSRT
                     IVPGFKVVAVTEEPFGAHPGYTPGFYDVDFSFGYLYQQASNTVEGFQAFLDEWVFGVK
                     NRTEYINHYIQKFGYEQFKKLWAKFDYSYPVSYAY"
     gene            4117..4917
                     /locus_tag="PK348_01190"
     CDS             4117..4917
                     /locus_tag="PK348_01190"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HXK64857.1"
                     /translation="MSEHPNDYIKPELMACCGAREIQDGDIVIVGTGFPTMSANIARY
                     THAPNAKLMQESGVYDARPLRPALSVGDPCLNPGAAMIGGLVDVMGMFLQGGWIDVGF
                     LAGSQVDKYGNINTTVIGDYNAPKSRLPGSGGANPIGSLAKKVLIISLHDTRHMAEKV
                     DFITTPGYIDGHGSREKWGLPANTGPSVIITNKAVLRFDKTTKVAYLESYHPDTSVDE
                     VVKLTPWKLEVSDNVHETEPPRAEELKALREILDPFRMIKIYEKRGYV"
     gene            4955..6319
                     /locus_tag="PK348_01195"
     CDS             4955..6319
                     /locus_tag="PK348_01195"
                     /inference="COORDINATES: protein motif:HMM:NF015905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HXK64858.1"
                     /translation="MEYTKILSDFCAKLTYNDIPEQVIHKAKLCLLDYIANVYGSLEI
                     EAVKHTIDFIMSITGKNTSTILGITKKTDLLSAAFVNGTTAEAIEAQDGLRFGGNHPV
                     CAVMPAALTVGEKYKKDGKSILTAIIAGYEVANRAAASVHPFHTLSGFLPTGTCGTFG
                     AATTAGKLMQLDEKGMLNAIGNAGYVVPLSMAEHLMGGYTIKIVQGGQAASAGIMAAG
                     LAAHGITGFPKVFEGSELKGGFAQITSKAEPKPERLIDKLGEHFTIMDIYIKPYTGCR
                     HTHGAVQGTLGLIQENNVKPDDVDSILVETYGIAELAVGKHITTNSSFVAAQFSIPYT
                     VSACIFDGDLGPAQITEKRISDSTLIEFSKKVTVKAADDLNAMYPDKTASRVTIKLNN
                     GQIIQKQVDIPKGDPRDPMNVEDIKQKIKRFAKHRKVNADALAEMVMNIEKIKNINEL
                     IKII"
     gene            6354..>7169
                     /locus_tag="PK348_01200"
     CDS             6354..>7169
                     /locus_tag="PK348_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015946871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXK64859.1"
                     /translation="MDFSYSEEQQMLIKLIHDLGEREKFRDLAREIDKTCQFPFQLIP
                     KYAELGLLGMTLSPEHGGGGQPLLNAIIAIEELAKYSPMIAAPVFESNVGPIKAIDIL
                     GSEFQKKKLIPGVCKGEYSVSVCMTEPEAGSDLTALRTNALEDGDSYVLNGRKAFITG
                     GGHATHYLVYTRFDNIKGYKGIGSLIVEKGMEGFSFGKQEVYMGLHGMPSCDLFFDNV
                     RVPKENVVLKPGEFNRLMATFHIERCGNAAMCLGIAGGALAEAKKYAMEREAFG"
BASE COUNT         2252 a         1151 c         1666 g         2100 t
ORIGIN      
        1 gactatctac ctaaaactga ttagctacta ctgattttta gaaaaattta aaaaagtgga
       61 tacagttctt tcaaatgtgg gtattggttt ttttggccct tttgaagaaa ttgatttaga
      121 aaaagccctt aaatgttttg aaatcaatgt cattggtgct gcagcaatat ttaaagcatt
      181 tatcccctat atgcgaagtc tgaaaagagg gaaattaatt gccatgtctt ctcttgttgg
      241 aagaatacct ttcccgtttg aatcaatata tacagcaaca aaatttgcct taacagggct
      301 tattgaatct atacggtatg aagttgagcc ttttggcatt aaagtggcac ttattgagcc
      361 tgcacaggtg tcaactaaat ttgcagcaaa aatacatgtg ttaccgccat ctagttcacc
      421 atattttgac cgggttaaac gtttcattaa cagggataat gaattaatta aaacagcacc
      481 tgatcctgta tatgctgcaa agaaaattta taacgtagtg atggcaaaaa atcccaggct
      541 ttacaatcag atagattttc aaagcacatt tttcctgtgg ctgaataatt ttttgcctgt
      601 aaagttaaga gacattatcc tgataaacta catggatatc aaaccaagat agccatagta
      661 gtttttcata tggagtttgg gtatattgaa tacagatttt tcagtgtatc ctgtgatgca
      721 cgtataagtg taagggaggt aataatatgg aaacattacg gaaaatagta cctcattatg
      781 atgattttga aagggtgaat acattgcgat ggagaataat taatgctcca caggaagttt
      841 gtattgaacg ggcacgatat ttgacccaat ccatgatgca tcactgggaa aagcctgcct
      901 taacaagaat gagcctggca ctggagcata tattgaataa tattagtgta atcattcgtg
      961 atgatgaact gatcgttgga tgcagaactt caaagttaaa aggtgctccg ttatttcctg
     1021 aaaacaagtc gcgctggatt gaaggcgatt gtgataattt tgataagcgc ctattacaga
     1081 aagcattgat tacccagaaa gaaaagcagg aactatcggc taatatactt cctttctggc
     1141 atggcaaaac agttgaagat atttttgaaa gccgattacc ccaagacgtc atggaagata
     1201 tggataaata tatctttacc atgatgcttg agataacgta cggcattggt cactttacca
     1261 tgaattattc taaggtttta catcttggtt tgaaagggat tatttcccat ctggaacaaa
     1321 aaatgcatga gcttgaggta cagggcgaag ctaatgaaaa gtggttgctg tatgatgcaa
     1381 tgatacgttc atgcaaggcg gcgatagtat ttgccaaacg atatgctcag aaagcacgag
     1441 aaatggctgg taaaacaaaa aggcgtaagc gtgcaaagga gttgcttgaa atagcacgta
     1501 tttgtgaaaa ggtgcctgaa ttccctgctg aaacatttca tgaagctgtc caatcgctgt
     1561 attttatcca tctggttact cagattgaat cgggaggcaa ttcggtttca attggaagga
     1621 ttgaccagat tttatatcca ttctatgaaa aggatatgaa atcaggacgt atcactcagg
     1681 ataaagcgcg tgagcttatt tcactgttgt ttatcaagat gaatgaaatc tggaatgttc
     1741 tggaggaggc atacatcccc ggtggggaag gtgcagaagg gaaaaccact cagaatgtaa
     1801 ccataggtgg agttgatagg caggggaatg atgttaccaa tgaactatcg tacatagttc
     1861 ttgatgctta tgctgatata cggacagtac agcctaattt cagtgttagg atttcaaaga
     1921 agacaccaaa agaattttta attaaagctg ttgaatatgc ccgtgatggt gtgttgatgc
     1981 acttttttaa tgatgagata gttattgaga ctttgatgaa agccgggcat tctcttgaag
     2041 atgctcgtga ctatgcgctt gttggctgtg ttgaaccaaa tgctcagggt aaatgttttg
     2101 gctcaacttt tgctgttcag ttcagcggta ttaaatgtgt ggaatttgct ctatccaatg
     2161 gtgttgataa tatttttgga tataaaagtg gaattgaaac aggcgacccc agcacatttc
     2221 atacgtttga cgatgtatgg aatgcttatg accgtcaggt gaaacatttc atgaatcaga
     2281 tggcgcgtgg catggagatt cttgataaaa ctatagctga acatgtacct tcaccatttg
     2341 catcagtgat gattgatggt tgtatagaaa agggaaagga tttaacatcg ggtggtgccg
     2401 tctacaattc aacaggagtg cagcttatag ggtttgccaa tattgttgat agtctgtatg
     2461 gagtaaagaa ggccgtgtat gaccagaagt actgcagtat agctgaactg gcacaatggc
     2521 tttctgatga ttggcaggat gttgaagata aaagagttta tttttttagg aaaatcccaa
     2581 aatttggaaa tgacaatgat gaagttgatg gattgggaat taaggtcctg gaacattttt
     2641 gtgatactgt aagcagccat aaaaatttca gagggggtac attctggcct ggtgtgtttg
     2701 tagtaggttt tcatataagc tttggtgcat ttactggagc tacggctgac ggtcgtcatg
     2761 caggagatgt tttaggtaat ggattaactc ccacaacggg aaatgcaatt tcaggtccaa
     2821 ctgccatcat gaactccata acaaagcttc cgttaacaaa aatatataat ggtgccaatc
     2881 tcaatatgag gttcaatggt aacaaaatat caacagaaca tttagcctat ctcattgaaa
     2941 catattttac aaaaggtgga atgcaggtcc aatttaatat ggttgattca aagatattac
     3001 gtgatgcaca gaagaatccg aaaaatacag ggatttattt gttcgcgtca gcgggtattc
     3061 ggctgaattt gtaggactat cagagatagc tcaagaagaa ataattagcc gcactgaatt
     3121 tgagtataaa ccataaaaat acatctattg attttattgg aggaaagtat gagcaaaata
     3181 atgaccacac aggaagcaat caccagattt gttaatgacg gtgattttct ttttattggt
     3241 ggttacatct gcaggccgcc atttgctgct atacatgaaa ttatacgcca gaagaaaaaa
     3301 gatctaactg ttgtccgcag taatactgct gatgattttg atatgctcat aggtgctggt
     3361 tgtgtaaaac ggtttatttg tacattcatt tcactgggct tgtatggcct tgcacggtgt
     3421 ttcagacgat caatagaaaa gggcattcct cacaaaattg aaattgaaga atatacaaat
     3481 ttatcattgc cactgatgct tatggcaggt gcgcttggta tgccgtttgt accggtaaag
     3541 gacatggtgg gtaccgattt aatgaagata aaatcattta tgggtgataa caaatataaa
     3601 tttattgatt caccatttga tggaagcccg gtaatgctgg tgcctgcact caatccagat
     3661 gttgcaatta ttcatgttca acaggcagat gaagaaggca atgcccagat gtggggtatt
     3721 ggtggtgatt gtaaatgggg agctaatgcg gcaaagaagg tgatagtaag ttgtgaaagg
     3781 attgtaagcc gtgaggttgt agggaaagac ccttcaagaa ccattgttcc cgggtttaaa
     3841 gttgttgcag taactgaaga accctttggg gcgcatccgg gttatactcc gggattctat
     3901 gatgtggatt tttcatttgg atatctttat cagcaagcat caaatactgt agaaggcttt
     3961 caggctttcc ttgatgaatg ggtatttggt gttaaaaaca gaacggaata tatcaatcac
     4021 tatatacaga aatttggcta tgagcaattt aaaaaattat gggccaaatt tgattacagt
     4081 taccctgtat catacgcata ttaaaggagg tttgccatgt cagaacatcc caatgattat
     4141 attaaaccag aattgatggc ctgctgtggt gcgcgtgaaa tacaggatgg agatattgtc
     4201 atagtgggaa caggattccc aacgatgtca gcaaatattg cgcgatatac gcatgctccc
     4261 aatgcaaagc tcatgcagga atccggtgta tacgatgctc ggccgctaag gcctgcttta
     4321 tcggtgggtg atccttgtct taatcccggt gctgcaatga ttggggggct tgttgatgtg
     4381 atgggtatgt tccttcaggg tggctggata gatgttggat ttctagctgg aagccaggtg
     4441 gataaatatg gcaatatcaa tacaacagtc attggtgatt acaatgcgcc aaaaagcaga
     4501 ctcccgggat ctggtggggc aaatccaata ggctcgctgg ccaaaaaagt gcttataata
     4561 tcactccatg atacccgtca tatggctgaa aaagttgatt ttattacaac accaggctat
     4621 atagatggtc acggttcccg ggaaaagtgg ggcttgcctg ccaatactgg tccttctgtt
     4681 attattacca ataaagcagt gctgcgtttt gataaaacaa ctaaagtggc atatttggaa
     4741 agctatcacc ctgatacaag tgtggatgag gtggtcaagc ttactccctg gaaactggaa
     4801 gtcagtgata acgttcatga aacagaacca cctcgtgctg aagaattgaa agcactccgt
     4861 gaaatactgg atccattcag gatgatcaaa atttatgaaa agcgtggata cgtataggca
     4921 ataataatat ttagatgtat aaaaggagga aagtatggaa tatacaaaaa tactttcgga
     4981 cttctgcgca aagcttacat ataatgacat tcccgagcag gtgatccata aagcaaaact
     5041 atgtcttctt gactatattg ctaatgtata tggttcttta gagatagaag ctgttaaaca
     5101 caccattgat tttattatgt ccataaccgg aaaaaataca tcaacaattt taggaattac
     5161 aaaaaagact gatctactga gtgctgcatt tgtaaacggt acaacagctg aagcgattga
     5221 agctcaggat ggtctgaggt ttggaggcaa tcaccccgtt tgtgcagtta tgcctgctgc
     5281 tctaactgtt ggggaaaaat ataaaaaaga tgggaaaagt atccttacgg caatcattgc
     5341 aggttatgag gttgcaaata gggctgcagc ttcagtgcat ccattccaca ccctttccgg
     5401 ttttttgcca acaggcacgt gtggtacatt tggggcagca acgactgcag gcaaactgat
     5461 gcagcttgat gaaaagggta tgctgaatgc aattggcaat gcagggtatg ttgtgccgct
     5521 ttcaatggct gaacatctta tgggtgggta taccattaaa attgttcagg gaggacaggc
     5581 cgctagtgca gggattatgg ctgcaggtct tgcagctcat ggtatcacag gttttccaaa
     5641 agtttttgaa ggctccgagc ttaagggtgg ctttgctcaa attaccagca aagcagaacc
     5701 aaagcctgaa aggcttattg ataagctggg tgaacatttt actattatgg atatctatat
     5761 taaaccatat acaggatgcc ggcataccca cggggcagta cagggaacat taggactaat
     5821 acaggaaaat aacgttaagc ctgatgatgt ggattctatc cttgttgaaa catatgggat
     5881 tgccgaattg gctgtgggaa agcatataac aaccaatagt agttttgttg cagcgcagtt
     5941 ctctattcct tatactgtga gtgcatgtat atttgatggt gatttagggc cagctcagat
     6001 tacagaaaaa cgtataagtg atagtacatt aattgaattt tcaaagaaag taacagtcaa
     6061 agccgcagat gatctgaatg ccatgtatcc tgataaaact gcaagccgtg ttacaatcaa
     6121 actgaacaat ggacaaataa ttcaaaaaca ggttgatatt cccaaaggtg acccgcgtga
     6181 tcctatgaat gtggaagata ttaagcagaa aattaaacga tttgcaaaac atagaaaagt
     6241 aaatgctgat gcgttggctg aaatggttat gaatattgaa aaaataaaaa atataaatga
     6301 gcttatcaaa attatttaat tacaaataaa attttagccc gggggtatta tacatggatt
     6361 ttagctatag tgaagaacaa cagatgctga ttaaacttat tcatgattta ggcgaaagag
     6421 aaaagttcag ggatttagca agggaaatcg ataagacatg ccagtttcct tttcaattaa
     6481 ttccaaaata tgctgagctt ggtcttttgg gaatgacact ttcgccagag catggtggag
     6541 gagggcaacc tttgttaaat gccattattg caatagaaga actggcaaaa tatagcccta
     6601 tgattgcagc tccggttttt gaatcaaatg ttggccctat caaagctatt gatattcttg
     6661 ggagtgaatt ccaaaagaag aaattaattc ctggtgtttg taagggtgag tacagtgtgt
     6721 ctgtatgtat gactgagcca gaggcgggat cagatttaac tgctttgagg acaaatgctt
     6781 tagaagatgg cgatagttat gttctcaatg gccggaaagc ttttattacg ggtggtggtc
     6841 atgcaacaca ttacctggta tatacccgtt ttgataatat taaggggtat aaaggtattg
     6901 gtagtttgat agtggaaaaa ggaatggaag gatttagttt tggcaagcag gaagtttata
     6961 tgggtttaca tggcatgcct tcgtgtgatt tgttttttga taatgttaga gtaccaaaag
     7021 agaatgttgt attaaaacca ggtgagttta acaggcttat ggcaacattt catattgaac
     7081 gatgtgggaa tgctgccatg tgcttaggaa ttgctggggg tgcactggca gaagcaaaaa
     7141 aatatgctat ggaacgcgaa gcgtttggc
//
LOCUS       DAOSZP010000027         7157 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_8878_length_7157_cov_3.458322, whole genome shotgun sequence.
ACCESSION   DAOSZP010000027 DAOSZP010000000
VERSION     DAOSZP010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 7157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7157
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8878_length_7157_cov_3.458322"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..598)
                     /locus_tag="PK348_01205"
     CDS             complement(<1..598)
                     /locus_tag="PK348_01205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK64860.1"
                     /translation="MRWILLVYLLTPSLVWGQLLINEIACNVPGSDWVELFLSSAQPL
                     SMDISSLYVTMYYGTNEPLSQNPVTIYSYDRAETPWDDRFVVVHLTDATTPDECDATG
                     DINGNGVLDVYCNNYVNSLWNTDCVVAIDTDDDPSNGGIIDFVAYSNRDGTLNQSIVQ
                     YVGAAIQHGQWLGTASEEYMVDIGKNGLQEYQSIIRKAA"
     gene            772..1404
                     /locus_tag="PK348_01210"
     CDS             772..1404
                     /locus_tag="PK348_01210"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HXK64861.1"
                     /translation="MVKLDNPYAKQRPSWKVFVPMLLVYPILKWVIHYSYFSFLYQYS
                     IALLILIIIAQYLDERLFTKDRIFSFDFPFERKIFYLFVMIIVLIPIRNIAVQFTPTP
                     QLLNFYQGKIKTLESVLIMVAIIAPCEELFFRGFAQYNLSFFFGKYGGVVIAALLYSI
                     TGIAAHSLYLAVLFFIIGIVIGLIYSRVQSLVQATLLHIITIIFMYLFPF"
     gene            complement(1401..1547)
                     /locus_tag="PK348_01215"
     CDS             complement(1401..1547)
                     /locus_tag="PK348_01215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK64862.1"
                     /translation="MYSVFVFIMVIASLLVLFILPRIESKPDKCDISNNWQSVWEERL
                     KIDN"
     gene            complement(1673..2425)
                     /locus_tag="PK348_01220"
     CDS             complement(1673..2425)
                     /locus_tag="PK348_01220"
                     /inference="COORDINATES: protein motif:HMM:NF015297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HXK64863.1"
                     /translation="MRLIENLEAKLDDLTHKYGLVALKGGTEVEDMTYDELLFLKQIA
                     INLPVIVKIGGPEARNDIRYCKSIAIDGILAPMIESEYALENFITAVLDIYKEATLPY
                     LAINVETITAYHNLSAIYTNPYFSYINQITVGRSDLSQSMKKRVDDDDVLYATANIVK
                     AAKLSGKITSVGGQVNPSNAEVVQKVIQPDRINTRHLVFDCKKADNISKSIQLGLEFE
                     LDLYRAFSNVEPSKSHIYQKRISVTSDRLAVV"
     gene            2794..2997
                     /locus_tag="PK348_01225"
     CDS             2794..2997
                     /locus_tag="PK348_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020875861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HXK64864.1"
                     /translation="MAKGTVKWFNESKGYGFLSQDDGKDVFVHHSGIAGDGFKTLFEG
                     DRVEFSIEQSEKGPRAVSVRTIR"
     gene            complement(3062..3481)
                     /locus_tag="PK348_01230"
     CDS             complement(3062..3481)
                     /locus_tag="PK348_01230"
                     /inference="COORDINATES: protein motif:HMM:NF022317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2784 domain-containing protein"
                     /protein_id="HXK64865.1"
                     /translation="MQIVYTLLDGFFLVFHSAFTIFNMFGWISVKIRKVHFFTMVATG
                     FSWFFLGIWYGWGYCFCTDWHWQIREMLGKPVPFNSYIQFLIYEITGYIPDANITDFF
                     VVTVYFMSLLISIVLNIRDYHNAKTYIILDEIKKPDC"
     gene            3644..3967
                     /locus_tag="PK348_01235"
     CDS             3644..3967
                     /locus_tag="PK348_01235"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HXK64866.1"
                     /translation="MLLSGTIVIDITQFISGSRCTQILADMGAEVIKVEHPKGDTLRL
                     IFQLMAGAERCYSVFNRNKYGIEVNWHDIRGCEIIKQLAAQADVFVHNLVPGSLERWL
                     FISAD"
     gene            complement(4019..>7157)
                     /locus_tag="PK348_01240"
     CDS             complement(4019..>7157)
                     /locus_tag="PK348_01240"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HXK64867.1"
                     /translation="NLVIIARGGGTGLTGGAIPLLPNTMIINTEKLNNIGKIENTTIN
                     GKTIPTITVEAGAVTEDVMEYCEHQNYIFATDPTSAWACTIGGNIAENAGGKKCVMWG
                     TCIDNILSFDIVDHNGDIITVRRVDHPYRKILPGDEVLFTVEKNKKLLKTITLSGLDI
                     RKKGLGKDITNKALGGLPGLQKEGCDGIIVNATFVLYRPFKYTRSVCFEFFGNNMINA
                     SKAIVEIVKTFEDDPVVFLTALEHFDEQYVKAIQYKNKSSRTEIPKAVLVVDIESDDE
                     AKLEKATEDLVAKVKQFNTEGIIAKDTETREKFWQDRKNLSAIAKHTNAFKLNEDIVI
                     PLEKLQDFSDFIEKLNVKKELENNIQIIRALIDYLQERVKTEEDDVCIERCHAGMGQL
                     LSMQSRYTDILNNLDTPAKDYFKYDSEYALRLNTVFQLIQNNELTMDFEKEVDEPLQK
                     LFYGYDDILEKILQVKEATGKRRIVVATHMHAGDGNVHVNIPVHSNDYLMMRDADETA
                     ATIMRQTVALGGVVSGEHGIGLTKIRFIDNETLEKFAEYNRYADPEDLFNPLKLTRDF
                     NLETIYTPSFNLLEGEAFILKATDLETVFNSIATCIRCGKCKSVCNTHYPDGMVFYNP
                     RNKILATALIMEAVLYDIQTSTSLSFKHFNNLREISNYCTICHNCQKPCPVAIDFGNI
                     TLNIRSILEERHKSTFKPVTSFTLFYLKQKGYYINKIFRIILLKWAYSMQRVGFYVAK
                     PVTHILNVIMPYIAMMLKGRLPKSGSKTLRDELKLKSSNTFYVFRNKNKPVVKTVVYF
                     PGCGSERMFPEISMATIALLYNAGVRVIIPPTYLCCGYPMKANGKLDQARIKTNENRV
                     IFHRMADTFSYMEIEDIVISCGTCYEMLSDYHLEDVFRGAKLIDINEFIAREGLYSLS
                     IQDSLIYHEPCHTPMKYMGYQKTFSKLFNIKPTAVPNCCGEGGTLALSTPDISNTLRE
                     RKETNIKTAIKKKNVMVLTTCPSCVQGLCKIQGNINVTGKSLVVYLAEQCLGKHWKKE
                     FIKEIRTQGFDRYIY"
BASE COUNT         2227 a         1360 c         1173 g         2397 t
ORIGIN      
        1 atgctgcttt tcgaataata ctttgatatt cctgcaggcc attttttcct atatcaacca
       61 tgtattcttc agatgctgtt cccaaccatt gcccatgttg tattgctgca ccaacatact
      121 gtacaataga ttgattgagt gtcccatccc ggtttgaata tgcaacaaaa tcgatgatac
      181 caccatttga ggggtcatca tccgtatcaa tagcaacaac acagtcggta ttccacaggc
      241 tgttaacata attattgcag tatacatcaa gtacaccatt gccatttata tccccggttg
      301 catcacattc atcgggagtt gtagcatctg tcaggtgtac cacaacaaaa cgatcatccc
      361 atggtgtttc agctctatca taactataga tggtaactgg attttgtgaa agcggttcat
      421 ttgtaccata atacatggtc acatacagtg aagaaatatc catagataaa ggttgtgctg
      481 aagaaagaaa aagctcaacc caatcacttc ctggcacatt acatgcaatt tcattaatta
      541 ataattgccc ccatactaaa gatggggtta aaagatatac taacagtatc cagcgcattg
      601 ttggctcctc atgttgctct ttttgtataa atacgctgga atgagaaaaa attaaaagtt
      661 tataagaaaa tttttatatt tttttaaaaa atcttatgaa ataatgaaaa aaaatataat
      721 acattgaatt aattgcttga ccaatcatat tttacagcaa atatatttgc catggttaag
      781 cttgataacc catatgcaaa gcaaagacca tcatggaaag tatttgtacc catgctattg
      841 gtatatccaa tattaaaatg ggttatccat tattcctatt tttcttttct ctatcagtat
      901 agcattgccc ttcttatttt aatcatcata gcccagtatt tagatgaaag attattcact
      961 aaagatcgta ttttttcttt tgacttccct tttgaaagaa aaatatttta tctatttgtc
     1021 atgattattg tccttatccc cattcgcaat atagctgtgc aatttactcc aacccctcaa
     1081 ctattgaatt tttatcaggg taaaattaaa acattagaat cagtgctgat aatggtagca
     1141 attatagccc cctgtgagga gctttttttc agaggttttg cccagtataa cctttctttt
     1201 ttctttggaa agtatggagg agtagttatt gccgccctgt tatacagtat aacaggcata
     1261 gcagctcatt cattgtattt agcagtgtta ttttttatta ttgggatagt aataggtctt
     1321 atctatagca gggtgcaatc actggttcag gctacattgt tacatataat aacaattatt
     1381 tttatgtacc tattcccctt ttaattatca atctttaaac gctcttccca gactgactgc
     1441 cagttattgc tgatatcaca tttatctggt ttactttcaa ttcttggcaa aataaacaat
     1501 actaaaagtg aagcaattac cattataaaa acaaatactg aatacataaa aaacctcata
     1561 aaaccattag caattaaagt aatattaatt gaggaaggat atcgtaagaa agagtgtttt
     1621 tagtcaagtt tttttaaaaa tattttttta acctaataat ttttatgtaa tatcacacaa
     1681 cagccagtct gtcagatgta acagaaatcc ttttctggta tatatgactc ttagagggtt
     1741 caacattaga gaaagctcta tataaatcca gttcaaattc aagcccgagt tgtatggatt
     1801 ttgagatatt atcagctttt ttgcaatcaa aaacaaggtg acgagtattt attctatctg
     1861 gttgaataac cttttgaacc acttcagcat tggaaggatt tacctgaccc cctaccgatg
     1921 ttattttacc agataattta gcagctttaa caatattggc agtggcatat aatacatcat
     1981 catcatctac acgctttttc attgactgcg acaggtcact tcgtccaaca gtaatttgat
     2041 taatatatga aaaatatgga tttgtatata tagctgaaag gttatggtac gcagtaattg
     2101 tttcaacatt aattgccaga tagggcagtg ttgcctcttt atatatatct aaaacggctg
     2161 taataaaatt ttctaatgca tattctgact caatcatagg tgccagaata ccatcaatag
     2221 ctatcgattt acaatatctg atgtcatttc ttgcttcagg tccaccaatt tttacaatta
     2281 caggaagatt aatagctatc tgttttaaaa agagcagttc atcatatgtc atatcttcaa
     2341 cttcagtgcc acctttgagt gcaaccaatc catatttatg cgtgagatcg tctaattttg
     2401 cttctaagtt ttcaatcagt ctcatagcaa ttgtcccttt ctatttttaa tttactttat
     2461 actcctatac gattaatcgg catttccaga aaaatgataa aataaattaa ggccgccatc
     2521 ttaccttagg cggttggaag gtacactttg tcatatatta ttcactaaat tatattcagt
     2581 aataacattg aaaaaaacgt aattttttaa gaaaaattta ttttatttga caattattac
     2641 gctatactta ctatgactag atagttggta tcctcttaat tacatcgttt ttccttcctt
     2701 atctattggc aagttaaaac agtacatgtg ggatgttcaa cggtaacagg tataatctcc
     2761 tgttataaca aattatttat ggagcgtcat acaatggcaa aaggtacagt aaaatggttc
     2821 aatgaaagca aaggttatgg ctttctatca caggatgatg ggaaagatgt gtttgttcat
     2881 cattcaggga ttgctggcga cgggtttaaa actcttttcg aaggtgatcg tgtagaattt
     2941 tctattgaac agagtgaaaa aggaccccgg gctgtttcag taagaaccat tcgataaata
     3001 caacatttgt aagttaaaag cttgttatat actaacacgt tgtatttggc aaaaaacccg
     3061 attaacaatc gggttttttt atttcatcga gaatgatata tgttttagca ttgtgataat
     3121 cccgtatatt taatactata gaaatcaata aactcataaa ataaactgtt acaacaaaaa
     3181 aatctgtaat attagcatct ggtatgtatc ctgttatttc ataaatcaaa aactgaatat
     3241 aagaattaaa aggaactggt ttccctaaca tttctcttat ctgccagtgc cagtctgtac
     3301 aaaagcaata tccccaccca taccatatac ccaaaaaaaa ccatgaaaat ccagttgcca
     3361 ccatagtgaa aaaatgaact tttctgattt ttacagaaat ccagccaaac atattaaata
     3421 tcgtaaacgc agaatgaaat accaggaaaa atccatctaa caatgtatac acaatctgca
     3481 taatgaagta tctcacttat tagcataatc aaaatataac taccgtgttc aaatgtaaca
     3541 attaattctc gcattccatt ttcatttata tatttcaagt ataaagttct tgatattcat
     3601 cagctttcat gtattcaata catagtgtca taaggagtat tgtatgcttt tatcaggcac
     3661 tatcgtgatt gatatcacac aatttatttc aggttcccga tgtacgcaga tacttgctga
     3721 tatgggtgct gaagtaatca aagtcgaaca tccgaaaggc gacactctcc gccttatttt
     3781 tcaattgatg gctggtgcag aacgctgtta ctcagtgttc aaccgaaata aatacggaat
     3841 agaggttaac tggcatgaca tcagaggttg tgaaattata aaacaactgg ctgcacaagc
     3901 agatgtgttt gtacacaatc ttgtgcctgg atcgcttgag cgttggttat tcatctcagc
     3961 agattgatga gttatacagg gataagatag tggtgtgata tgtgtgagca gcgtttaact
     4021 aataaatata tctgtcaaaa ccctgtgtgc gtatttcctt aataaattct tttttccagt
     4081 gtttacccaa acattgttct gccaaataca ccactaacga tttcccagta acattgatat
     4141 ttccctgaat cttacataga ccttgtacac agcttgggca ggttgtaagc accatcacat
     4201 ttttcttttt tattgcagtt ttgatattgg tttcttttcg ttcacgcagt gtattggata
     4261 tatctggagt tgataatgca agggttccac cttcaccaca acagttagga actgctgtgg
     4321 gttttatatt gaataattta gaaaatgtct tttgatatcc catatatttc atgggagtat
     4381 ggcacggctc atggtaaatc aggctatctt gtattgacag tgagtataat ccttcacgtg
     4441 ctataaattc attgatatca ataagttttg cacccctaaa aacatcttca agatgataat
     4501 cggataacat ttcataacat gttccacacg aaatcacaat atcttcaatt tccatgtaag
     4561 agaatgtatc agccattctg tgaaatataa ccctgttttc attggttttt attcgggcct
     4621 ggtctaattt gccattggct ttcattgggt aaccgcaaca caggtacgtt ggcgggatga
     4681 tgactctaac acctgcattg tagagaaggg caatggtagc catactgatt tcaggaaaca
     4741 ttcgttctga accacagcca ggaaaatata ccacggtttt tactacaggt ttatttttat
     4801 tcctgaatac ataaaatgtg ttgctgcttt ttaatttcag ctcatcacgc agagtcttac
     4861 tacctgattt tggaagcctt cctttaagca tcattgcaat gtacggcatg ataacattga
     4921 ggatgtgggt cacaggcttg gctacataaa agcccacccg ctgcatactg tatgcccatt
     4981 tcagaaggat aattctaaat attttattaa tataatatcc cttctgcttt aagtaaaata
     5041 atgtgaacga ggtaaccggc ttaaaggtag atttgtgtct ttcctccaag atagaacgga
     5101 tattcaacgt aatgttacca aaatcgatag caacaggaca tggcttctgg cagttatggc
     5161 atattgtgca atagttggat atctcacgca aattattgaa atgcttgaaa ctcaacgatg
     5221 ttgaggtctg tatatcatac agaacagcct ccataataag agctgtcgcc aatattttgt
     5281 tacgtggatt atagaagacc atcccatcag ggtaatgtgt attgcataca gatttacatt
     5341 ttccacaacg gatgcatgtt gcaattgagt tgaatactgt ttctaagtct gttgccttta
     5401 aaatgaaggc ctcaccttcc agaagattaa acgatggcgt atagatggtt tctaagttaa
     5461 aatcccttgt aagttttagc ggattaaaca gatcctcagg atcagcatag cggttgtatt
     5521 ccgcaaactt ttccaatgtt tcattatcaa tgaatcgtat ttttgtaagc cctatgccat
     5581 gttcaccact gaccacacca cccagtgcta ctgtctggcg cataatggtt gctgcagttt
     5641 catcagcatc acgcatcatg agataatcat ttgaatgtac gggaatattg acatgcacat
     5701 taccatcacc cgcgtgcata tgggtagcaa caacaatgcg gcgcttccct gtagcttctt
     5761 ttacctgaag tatcttttcc agtatgtcat cataaccgta aaaaagtttt tgtagtggtt
     5821 catccacttc cttttcaaag tccatagtca gttcattatt ctgtatgagc tgaaaaacag
     5881 tattcagcct gagtgcatat tccgagtcat acttgaaata atccttagct ggagtatcaa
     5941 gattattaag aatatcagta tagcgcgact gcatcgacag taactgtccc attcctgcgt
     6001 ggcagcgttc aatacatacg tcatcttctt cggtcttgac tcgttcctgg agataatcaa
     6061 taagtgcgcg tataatctgt atattattct ccagttcttt tttaacattt aatttttcaa
     6121 taaaatccga aaaatcctgc aacttttcca gaggaataac aatgtcttca ttcagcttaa
     6181 acgcattggt atgttttgca atagcactta aattttttct gtcctgccag aatttttccc
     6241 tggtttcagt atcttttgca ataatccctt cagtattgaa ttgcttaact ttcgctacca
     6301 gatcctctgt agccttttct aactttgctt catcatcgct ttcaatatca actaccagta
     6361 ctgctttagg aatttcagta cgagaagatt tattcttata ctgaattgct ttcacatact
     6421 gttcatcaaa atgttctaaa gctgttaaaa acacaaccgg gtcatcctca aaagtcttta
     6481 ctatttcaac gatagctttt gaggcattga tcatgttatt gccaaaaaac tcaaaacata
     6541 cagaacgggt gtatttaaac ggcctgtaca gaacaaacgt tgcattaaca ataatgccat
     6601 cacaaccttc tttctgcagt cctggtaatc cacccagtgc tttattggtg atatccttcc
     6661 ccaggccttt tttacgaata tcaaggcccg ataaggttat ggtttttagt aattttttat
     6721 ttttttctac agtaaaaaga acctcgtcac caggcaatat cttccggtag ggatgatcta
     6781 ccctgcgtac agttatgata tcaccattgt gatctacaat atcaaatgaa agaatattat
     6841 caatgcaggt accccacatg acgcacttct taccacctgc attttctgct atattaccac
     6901 caatagtgca tgcccatgcg gatgtagggt cagtagcaaa tatataattc tgatgttcgc
     6961 aatattccat tacatcttcg gttactgcac cagcctcaac cgtaatggta ggaatagttt
     7021 taccattaat agtcgtgttt tcaattttcc caatattgtt caatttttca gtattgatta
     7081 tcatagtgtt aggcaaaaga ggtattgcac caccagtaag tcctgtacca ccaccacgtg
     7141 caataataac aagattc
//
LOCUS       DAOSZP010000028         7126 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_8928_length_7126_cov_3.790270, whole genome shotgun sequence.
ACCESSION   DAOSZP010000028 DAOSZP010000000
VERSION     DAOSZP010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 7126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7126
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8928_length_7126_cov_3.790270"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="PK348_01245"
     CDS             complement(<1..215)
                     /locus_tag="PK348_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002753866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HXK64868.1"
                     /translation="MLLDEKLFDKFGVEYEPNDIIFCEFEPGNDFYFIQSGRVEIVKI
                     INNREKTLDILGPGDVFGEMSILDEEQR"
     gene            complement(225..1376)
                     /locus_tag="PK348_01250"
     CDS             complement(225..1376)
                     /locus_tag="PK348_01250"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXK64869.1"
                     /translation="MSNGSPTQGITTRTYKPGSIVYFENDKSENIYILKSGLIILTSQ
                     KLETGEEVKEEVRPGEFFGVKSALGRYPREETAQVIKESVVLVLTLADFERLILKNVN
                     VVLKMLRIFSNQLRRIGRAVREVLGETSTINPEEELFKIGEYYYRAGRFNQARYAYNK
                     YLDYYPNAKYSDLATKRIREIDSGQAESNIAPQPVKRKEVEPSTEELKDFTLDEDIDS
                     MPAKESVVDEMDDFLGENDSASSDFDIADESPKHEDISSMFYDAMSLYSQSEYESALQ
                     IYKKILNQKSLKNDAENKIYQKAHFEIGRTFFKMGQYNEAIEAFSLLMKKFPQSELNV
                     NALYHLAMIYEVKKQFDKAKSYYQKVISLAAKEEIGKQASRRLKNLESR"
     gene            complement(1394..2611)
                     /locus_tag="PK348_01255"
     CDS             complement(1394..2611)
                     /locus_tag="PK348_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016549465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXK64870.1"
                     /translation="MSEWSIAGKGILTPDKFVKKASILVSDGIIDKIDGRSSFSIEVD
                     GIIVPGLINSHDHLLGTYHPKVGNGPYENWLPWDNDLKSSPIYEERQQIENRDLYLLG
                     GYRNLLSGVTTVSDHIPHFVNEPFIDILPVKALKNYALAHAVVSFALAWGDGITLEYQ
                     KAAKEHIPFITHSGEGFDDETRHDIDTLERLGALGEYTVLIHGIAFNEQDIKRIKKAG
                     ASVVWCGDSNMYMFNRTANVKLMLEHGVNVCIGTDSPMSGGENLLYEIKFDRKLYKKL
                     FGQELSDETIVKMITVNPSYAFKLHDHGAIKPGNVADFIVISRMEDNPYSAVVNAQLE
                     DIALVVINGKPMYGDSSYKKLFDHNKVKYQEIILRNRHKLVVGDLLGLLRRISKAVGY
                     KKEFPFIPVEFDI"
     gene            complement(2896..3885)
                     /locus_tag="PK348_01260"
     CDS             complement(2896..3885)
                     /locus_tag="PK348_01260"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HXK64871.1"
                     /translation="MVRNIGIVIVSIIIIAIVLDAIVSILSRPQAGRKACQDAGIPYK
                     TVIAHRGASYWAPEETYFSYSIAKNVGADYLEMDVQRTKNDVIIVFHDDTLNRITNVA
                     EVFPGRENDPINSFTIAELKRLDVGKWFNTSFPERSRHSYSGTQILTLDEVIDIATSD
                     KGLYPLYIETKSPKNYPGIEEQLVHILQTRGYIENENKINAPYAPVIFQSFEIDSLLK
                     LQKLAPQVPRIYLIDEDMVNQKGWDTLIAEAKQAQCAGIGPSGYLGWPWITGKAHRNG
                     LLVHIYTVNKKWQYRLLDWFGADGFFTDRCELLMEYYVKPHKKPFKEILANID"
     gene            complement(3945..4226)
                     /locus_tag="PK348_01265"
     CDS             complement(3945..4226)
                     /locus_tag="PK348_01265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK64872.1"
                     /translation="MPRKEYISGTFCIDIKCTHHKALEQYSGDEYFKQKALLCADCYA
                     WKFFTWLRDHKYRIIQTLPEISARELAARIKGIDPVRVEDLTEDEILCL"
     gene            4597..4986
                     /locus_tag="PK348_01270"
     CDS             4597..4986
                     /locus_tag="PK348_01270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK64873.1"
                     /translation="MKRLEILNNYLATHTVPELVAKKLDANSFLTNNFSYHALRIGNS
                     IGDNLDISIEIIILDEIAKKYNLILNTTEHAELHTQGITEADLDSLVQAAILFENIKN
                     NKKQYKEILRKISYFIRKEFYPVIHQD"
     gene            complement(4987..6450)
                     /locus_tag="PK348_01275"
     CDS             complement(4987..6450)
                     /locus_tag="PK348_01275"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HXK64874.1"
                     /translation="MNKHTDKIFSLIESTPFLSNIIKKGGIQLKWVVTVSMLMVIVIA
                     VISFLLISISETALIKANDKLCQTIAYNISSSESFLTAEKKPLKRSMILQDIVKGLSK
                     TNIEGFVYAAIYDTQGIIAEKKYTYAAHSNAKLHGKYYSKDLLKQFESIHTTVKEKIE
                     LTIDSKPTVCYRYRVPFEFFKAKIGIIEIVFTEDAILGPVHRARLVMLIVAVILLSLS
                     IYAAMKIARQMVEPIVQLTSGVVRVKNGDLTTRLTITTHDEIGILTEEFNEMINHLRE
                     KLKMEKFVSESTISMIREKRDDELDLGGTRKNMAFLFSDIRGFTAMSEKLPPEEVVKI
                     LNEYLDLQASIIKKHGGDIDKFVGDEIMAVFEDKKKADSAVEAAVDIIESIENLNKQR
                     AKEGKITVTVGIGIHMGDIVHGRMGSKERMDFTSIGDAVNLAARLCSHADSQTILVSQ
                     EIIANLTKRKFKGKKLEPIKVKGKEHPIPIFQITGKA"
     gene            6833..>7126
                     /locus_tag="PK348_01280"
     CDS             6833..>7126
                     /locus_tag="PK348_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010419457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HXK64875.1"
                     /translation="MIINHNISAIFAHRTLKFHDWAITKDMEKLSSGMRINKAGDDAS
                     GLAVSEKMRSQIQGLRQAERNTEDGMSMIQTAEGYLQEVHDILQRIRVLAVQSA"
BASE COUNT         2150 a         1361 c         1218 g         2397 t
ORIGIN      
        1 cgttgttctt catcaagtat tgacatttcg ccaaatacat ccccagggcc gagtatatca
       61 agagtttttt cccggttgtt gatgattttt acaatctcaa ctcttcctga ttgaataaag
      121 taaaagtcat tgccaggttc aaattcgcag aaaattatat cgttgggctc atattctacg
      181 ccaaatttgt caaatagctt ttcatcaagt aacatagcat acctttaccg tgattcaaga
      241 ttttttaatc gccgtgaagc ctgtttacct atttcctcct tggctgccag agatatcact
      301 ttctgataat atgatttagc tttatcaaat tgtttcttta cttcataaat catagctaaa
      361 tggtataatg cattaacatt taattcagat tgtgggaatt ttttcattaa caaagaaaaa
      421 gcttcgatgg cttcattgta ttgacccatt ttaaaaaatg ttcgtcctat ttcaaagtgg
      481 gctttttgat aaattttatt ttccgcatca ttttttaatg acttctggtt taagattttt
      541 ttataaattt gtaatgcaga ttcatattcg gattgtgaat acagactcat agcatcataa
      601 aacatggagg atatatcttc gtgttttggg ctttcatcgg caatgtcaaa atctgaactt
      661 gcagaatcat tttcacctaa aaaatcgtcc atttcatcca ctactgattc ttttgccggc
      721 attgaatcga tatcctcgtc cagggtaaaa tcttttaatt cctctgtgga aggttccacc
      781 tccttacgtt ttactggttg aggtgcaata tttgattcag cctggccgga atcaatttcg
      841 cgtattcgct tagttgctaa atcagagtat tttgcgtttg gatagtaatc aagatattta
      901 ttatatgcgt accgtgcctg attaaagcgg ccagctctat aataatattc accaattttg
      961 aacaattctt cttcaggatt aatagtgctt gtttctccta atacttcacg tactgcgcgc
     1021 ccaattcgtc tgagctgatt gctgaagatc cggagcattt ttaaaaccac attgacattc
     1081 tttaaaatga gacgttcaaa atcagcaagt gtcaaaacca gcaccactga ttctttaatc
     1141 acctgtgcag tttcttcacg cgggtaacgc cccagtgctg atttcacacc aaaaaattca
     1201 ccaggtctta cttcttcttt gacttcctct ccggtttcca gcttttgaga agttaaaatt
     1261 attaggcctg atttaaggat ataaatattt tcacttttgt cattttcaaa ataaacaata
     1321 gagcctggtt tataggttcg tgtggtaatc ccttgagttg gtgaaccatt tgacatagaa
     1381 atcctcgcac acgttatata tcaaattcaa caggaataaa tggaaattct ttcttataac
     1441 caactgcttt gcttattctt ctcagtaacc caagtaaatc gccaacaacc agcttgtgcc
     1501 tgtttcgcag gattatttcc tggtatttga ctttattgtg gtcaaaaagt tttttatagg
     1561 aactatcgcc atacattggt tttccattaa taaccaccag tgcaatatct tcaagctgag
     1621 cgtttacgac tgctgaataa ggattatctt ccatacggga aatcacaata aagtctgcaa
     1681 cattgcccgg ctttattgcc ccatggtcat gtaatttaaa cgcataggag ggatttaccg
     1741 tgatcatttt tactattgtt tcatcactga gttcctgacc aaaaagcttt ttgtataatt
     1801 tacgatcaaa ttttatttca tataaaagat tttcgccacc ggacatggga gaatcagtgc
     1861 ctatacaaac attgacacca tgttccagca taagcttgac atttgctgta cgattgaaca
     1921 tatacatatt ggaatctccg caccacacaa cagatgcacc agcttttttt atacgcttga
     1981 tatcttgttc attgaaagct attccatgaa tgagtacggt atattcaccc aacgctccta
     2041 aacgttccaa tgtatcaata tcatgacggg tttcatcatc aaaaccttca cctgagtggg
     2101 taatgaaggg tatatgctct ttagctgcct tctggtattc cagtgttatg ccatctcccc
     2161 aggccaaagc aaaagaaaca accgcatgag ccagcgcata atttttcagt gctttaaccg
     2221 gcagtatgtc aataaatggc tcattgacaa aatggggtat atggtctgaa acagttgtta
     2281 ctcctgacaa aagattgcga tagccaccca aaagataaag gtcacggttt tctatttgct
     2341 gacgctcttc atatattgga gaacttttaa ggtcattatc ccaggggagc cagttttcat
     2401 agggcccatt gccaacttta gggtggtaag tgcccagtaa atggtcatgg ctgtttatga
     2461 ggccaggaac aatgatgcca tcaacttcaa tagaaaaact actacggcca tcaattttat
     2521 cgattatacc atcgctgacc agtatacttg ctttcttgac gaacttatcc ggagttaata
     2581 tccctttccc agctattgac cattcactca ttaaacatct cctgtttaga agataaaaat
     2641 aaaattgtat tttttaaatt tatactatac tatattgata gtcaagtaaa atgtaatgtg
     2701 acccattact ttacatatcg gtcatttaat gtgcagcata tagctttttg tatatgtact
     2761 ctcgccattt tcctgttaat aaagtgcata tgcatgtatg gttcaagcac gtttaaactg
     2821 tgaaaatcaa aagttataat taacctgaaa aaaaggtttt tgacagaaac tacctttaat
     2881 actatgaata ccaatttaat caatatttgc taaaatctcc ttaaatggtt ttttatgggg
     2941 ctttacataa tattccatca acaattcaca tctgtcagta aaaaatccat ctgcaccaaa
     3001 ccagtctaaa agacgatact gccatttttt attcacggta tatatatgaa caagaagccc
     3061 attacggtgg gctttgccag ttatccatgg ccagcctaaa tatcctgatg gtccgatgcc
     3121 agcacattgt gcctgttttg cttcagcgat aagggtatcc catcctttct ggttaaccat
     3181 atcttcatca atcagatata tacggggaac ctgtggtgct aacttttgaa gctttagcaa
     3241 actgtcaatt tcaaatgatt ggaatattac tggtgcatat ggggcattaa ttttgttttc
     3301 attttctatg tatccgcggg tttgaagtat atggacaagt tgttcttcaa tgccagggta
     3361 atttttgggg ctttttgttt caatatataa ggggtataat cctttatcgg atgtagctat
     3421 atctataact tcatcaagcg taagtatttg tgtcccactg tatgaatgcc tggatcgctc
     3481 ggggaatgat gtgttaaacc attttcccac atccaacctt ttcagctccg ctatagtaaa
     3541 actgttgatt ggatcatttt ctcttccggg aaaaacttcg gcaacattgg ttatccgatt
     3601 taacgtgtca tcatgaaata caataataac atcattttta gttctttgca catccatttc
     3661 taagtagtcg gcaccaacat tttttgcaat actgtatgaa aaatatgttt cttctggtgc
     3721 ccagtacgat gctccacggt gagctataac agttttatac gggatgcctg catcctggca
     3781 tgcttttctg cctgcttgag gccgtgaaag tatggacaca attgcatcca gtacaattgc
     3841 aataataatg atagaaacaa taactatccc tatgttacga accattgtat acctccgtag
     3901 caatttgaaa aatctgtgaa atgtacgtat ttatattctt gatactataa acacaatatt
     3961 tcgtcttcag tcaagtcttc aactcttaca ggatcaatgc ctttaatgcg tgcagccagt
     4021 tcacgagcag agatctcagg taatgtttgt attatgcgat acttatgatc gcgaagccat
     4081 gtaaaaaatt tccatgcgta acaatccgcg catagaagag ctttttgttt gaaatactca
     4141 tcaccagaat attgttccag agctttgtga tgtgtacatt taatgtcgat gcaaaaagtc
     4201 cctgaaatgt actcttttct tggcataagc acccctatat acattattag attttattag
     4261 cattgtaaat tatcgtttta aaattgcaaa taaaaaataa gggggcttct tttcccaatc
     4321 atggtcttta gaataggata tatatcagta actatcacca aaagttaatg tgaacaatga
     4381 atggttcccc cattatcccc ttatgtacca agcatactct aatttatttt gttgcttttt
     4441 tacggtacct attggatgat tataaaaact gacaatgtac tacagcggta attcatatac
     4501 aggtactaat aaatagatcc cggaatcgat gttatttgtt tggaggaagg ggaaaagatt
     4561 gatagcatat aatttttttg atgggggaat agtataatga aacgattaga aattttgaat
     4621 aattatttag caacacatac cgtcccggag ttggttgcta aaaagctgga tgcaaattcg
     4681 tttttgacaa ataatttttc ctaccatgct ttgcgaattg gcaattccat tggcgataat
     4741 cttgatattt caattgaaat cataattctg gatgagattg caaaaaaata taacctgatt
     4801 cttaacacca ctgaacatgc agaactgcat actcagggca taactgaggc ggatctggat
     4861 agtctggtgc aggctgcaat attgtttgaa aatattaaga acaataaaaa gcaatataaa
     4921 gaaattttac gaaagatcag ctactttatc agaaaagaat tttatcctgt tatacatcag
     4981 gattaatcaa gctttaccgg ttatctggaa aatgggtatg gggtgttcct tgcctttgac
     5041 cttgataggt tcaagctttt tacctttgaa ttttcttttg gtgaggttgg caataatttc
     5101 ctgcgatacc aggatggttt gcgaatctgc atgggagcat agccgtgctg ccaggttgac
     5161 tgcatctcct atagatgtaa aatccattcg ctcctttgaa cccatccttc catgaacaat
     5221 atcacccata tggataccaa ttcccacagt gacagttatt ttgccttcct tagctcgctg
     5281 cttgtttaga ttttcaattg attcaatgat atcaacagct gcttcaacgg cgctatcggc
     5341 tttcttttta tcttcaaata ctgccattat ttcatcgcca acaaatttat caatgtcacc
     5401 accatgcttc ttaattatgg acgcctgcaa atcaagatat tcattgagaa tttttacaac
     5461 ttcttcaggt ggaagctttt ctgacatggc cgtaaagccc ctgatatctg agaaaagaaa
     5521 cgccatattt tttcttgttc caccaagatc aagttcatcg tctctttttt cacgaatcat
     5581 ggaaatagtt gattcggaaa caaatttttc cattttgagc ttttcccgca ggtgattgat
     5641 catctcatta aattcttctg tcaaaatacc aatttcatca tgtgtggtta tagtaagacg
     5701 tgtagtgagg tcaccatttt taacccgtac aacacctgat gtcaattgca ctattggttc
     5761 taccatttgc cgtgcaattt tcattgcggc atatatgctt aaggacaaca atatgactgc
     5821 aacaatgagc attaccagcc gtgctctgtg aaccgggcca aggattgcat cttcagtaaa
     5881 aactatctca ataattccaa tcttagcttt aaaaaactca aaggggacac gataccggta
     5941 acatactgtt ggtttagaat caatagtcag ttcaatcttt tctttaacgg tagtatgtat
     6001 tgattcaaat tgctttaaaa gatcttttga gtagtatttg ccatgaagtt ttgcattgct
     6061 atgggctgca taggtatact tcttttcagc tataattccc tgagtatcat aaatggcagc
     6121 atagacaaat ccttcaatgt ttgtttttga aagccccttc actatgtcct gtaatatcat
     6181 ggaacgcttt aatggcttct tttctgcagt taaaaaactt tctgaagagg atatattata
     6241 ggcgatagtc tgacataatt tatcattggc ttttatcagg gctgtctctg atatacttat
     6301 caacaaaaag cttattactg caataactat taccattaac attgatacgg tcacaaccca
     6361 ctttaactga atgccacctt tttttatgat gtttgataga aagggagttg attcaatcag
     6421 ggaaaatatc ttatcagtat gtttgttcat ataacctctt gatgattttc tattgaaata
     6481 ttacagtata cactatttac caatgtttat ttatattcaa gaaaaagatt ttaaaaaatt
     6541 tccactatta atttctcatc tatgatttat gaacataata tcaataatga gcaattctgc
     6601 tgactgaagc agtatgagtt tataatgcag tattaataaa agttttttta tattatataa
     6661 aacaataata actctataat ataataacat atattgtaat aaacgtctat aaaagcttat
     6721 aaatgacaat attctatctc agtcgttttt ttcaataaga cgaactttgg aatgaactat
     6781 ttatatagtt aatgctatca cggatgatag tatatctttc aaggaggata ccatgattat
     6841 aaaccacaac atcagcgcaa tttttgccca ccgaacgctg aaatttcacg attgggcaat
     6901 caccaaggac atggaaaagc tctcatcggg gatgcgcatc aacaaagcag gtgatgatgc
     6961 atctggatta gccgtgtccg aaaaaatgcg ttcccagatt cagggattac ggcaggctga
     7021 acgcaatact gaagacggta tgtcaatgat tcaaactgca gaaggatatt tgcaggaagt
     7081 acatgatata ctgcagagaa tcagagtttt agcagtacag tctgca
//
LOCUS       DAOSZP010000029         7107 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_8952_length_7107_cov_3.836500, whole genome shotgun sequence.
ACCESSION   DAOSZP010000029 DAOSZP010000000
VERSION     DAOSZP010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 7107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7107
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8952_length_7107_cov_3.836500"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2139)
                     /gene="infB"
                     /locus_tag="PK348_01285"
     CDS             complement(<1..2139)
                     /gene="infB"
                     /locus_tag="PK348_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002677632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HXK64876.1"
                     /translation="MKAIDVATKNHVSTDDVIKICKDLGIPCTDDQSELANDDVFLIE
                     KKIQIIKEQRAQEAKKLIQQAELKKKIKLKRKVHVAKELKKEAEAKEDEATEEVVEHK
                     EDSKEVAPKHRAETGVVEKEKQEKKERFDKKERHGKPKDRFPKKDFRKDDKQRPGAEE
                     KEQRPERQHAPTVVEKRDVPVIDKEVVDLQKRQKEKGKAKEKDRDKDKRFLKKEKEEL
                     EEKLLLLKRKKESQPREAVAPKEIEITETITVGDLAKKMNIKASEVIAKLMSMGVMAT
                     INQVIDADTASILASEYGTEVKVVSLYEETVIKVDEEDRPQDYVSRPPIVTVMGHVDH
                     GKTKLLDAIRETNVVATEHGGITQHIGAYRVKVEDKYVTFLDTPGHEAFTTMRARGAS
                     VTDIVVLVVAADDGVMPQTIEAINHAKAANVPIIVAINKIDLPEANPNRVKQELTAYE
                     LIPEEWGGTTLFAEISAKQKINIHELLELILIQAEMLELKANPKLRARGVVIESKLDP
                     GRGPIATVLIQNGTLHVGDPFVVGLYSGRVRAMFTDTGEQVQEATPSMPVEVLGISGV
                     PSAGDPFQVVESEKYARQIAQKRQELVRLESAQKVKKVTLEDLNTMIKEGEIKELKIV
                     IKADVDGSVQALRDSLQKLSTNDIRVKVIHAATGGINESDVMLASASNAIIIGYQVRP
                     SAKVAEIAEKESVSIKYYNIIYDANNDVRAA"
     gene            complement(2266..3591)
                     /gene="nusA"
                     /locus_tag="PK348_01290"
     CDS             complement(2266..3591)
                     /gene="nusA"
                     /locus_tag="PK348_01290"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HXK64877.1"
                     /translation="MSGNFFEALHQIATEKGITREMIEEIVQSAMLSAYKKQYGITKP
                     VDITFDRERNTVKIISRKMVVRVPKNYAEEISLEEAQKIKPDAKFGDEVIVEENPLET
                     FGRIAAQTAKQVILQKIKEAEKNIVYDEYKDKVNELINGYVQRKTKEALYIDLGKTEG
                     IIPAREQSPLEHYKIGDRVKAIVLSVQKNSKGPSVVLSRAHPKFVEKLFEMEIPEIYD
                     GIVKIVNIVREPGMRTKVAVTSDRDDIDSVGACVGMKGVRIQSIVRELEGEKIDVIEY
                     SPNKKVMASNALTPAKVKEIIETRDGGVIAVVDNDQYRLAIGKNGHNARLAAKLCGFN
                     IDIKTEDQYREFLSSSESRALVEQLFATTSEGETPLEELPGLDARTITLLEAGGIFSV
                     EDLVEKSVEDLMNIDGIGEKTAQKIINIIQEYVDFEEEDYEDEQEDKQE"
     gene            complement(3603..4043)
                     /locus_tag="PK348_01295"
     CDS             complement(3603..4043)
                     /locus_tag="PK348_01295"
                     /inference="COORDINATES: protein motif:HMM:NF014617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK64878.1"
                     /translation="MKLNESLELIINNVAHDLGYSVYDSVLVLHGKTPRITVKIDHIN
                     GISHLDCEKFSKELSHRIDEGQLLDDYTIEISSPGIKRELRTIEDFLRFTGAPVRGIY
                     QEDDARKTFKGTLVGIEGEIVTINETNKVLTLSYSQIIKANLDY"
     gene            4303..>7107
                     /locus_tag="PK348_01300"
     CDS             4303..>7107
                     /locus_tag="PK348_01300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK64879.1"
                     /translation="MERDILQSIKLEITKNLKFTPYLRICLHPFIAQSKSDIAHNILC
                     AELSAEPAIRFSAIRTITHYKLPGFTDLFHALFQHSITDDEKIQICTYLATYGNSQTV
                     ELLNNYIMDNFNKESNHTIVIQCLETLRILRHPDSKLLATLKSIINETGTHEVIRYYA
                     IRALSIYDDIHVLSSLINQNEYTLLGIFDAIAFISDYCITKKTQKNEASDSSKEENLI
                     IELRVFLSKMLPHFDEFSTRVKISCLNALITSKHRETNDYVLKILGGQNENEKEELLL
                     LLQHTIMLLRDPEPLIRALISYGTVSPHHNTIIVDTIINYFESFQSDRSSTLLKDKLF
                     NYFTVTLDSFFELYRKNYMISDVEEKNYPEIFREVRNFILLKFSPQILNRIIHYLKHE
                     KNDEIHKIITLLTTYLPFIDSSIRDPFSSLVEMLYDRDPKSREITASRLETIDFEKRF
                     LQERIIRLCNIIAKLNIQSAATLLVKIYNYLKKYRDEKLFDACIQTLSTMKYPYMLGE
                     LELMLLSGDRNDQLFSLKYLAHYTDQQAASILFELLKNTANLDPEVIVTALHLLLQDE
                     TAQYKNSSEILKNIALTYNDIAIKQAAIRNIGHCGDEKEMEWLITLFTETNEISIKET
                     ILQSISSIIPRLRDFNKRALSQFLLDCLKESGIRIRIYAIAILLQLNNKEVAKYIKEM
                     LIIKNRDIQTEMLYIFHNYNTPEFSYFLLSLLKEEYAIAPETVSILQNLPAEISEDIV
                     NFIGNMYRKNGIDISQAEATLTIKPGKIDTLSDFFIITITTYGDTNPILLEKLATTLN
                     TIQSLILNHCKKNNLVIHALLPGLITVYSNNPLNIADALIAIVQNIQQYNDTSHIPFK
                     AIIQSYNARVIQSGQDIIIVSDAQYAHDILYNHAIIDEKLYSAIHDEFTCNPLPHILA
                     NHAHIPLFYLSNKKNSLVEAH"
BASE COUNT         2121 a         1548 c         1072 g         2366 t
ORIGIN      
        1 ggctgccctg acatcattat tggcatcata gatgatatta taatacttaa ttgacacact
       61 ttctttctcg gcaatttctg caacctttgc tgatggtctt acctgatatc ctattatgat
      121 agcatttgaa gccgaagcca gcataacatc cgattcatta atgccgcccg ttgctgcatg
      181 aattacttta acacgaatat cattggtgga aagcttctgc aacgaatcgc gcaatgcctg
      241 aactgaacca tcaacatctg ctttgattac aatcttgagt tctttgatct cgccttcctt
      301 aatcattgta ttcaggtctt ccagagttac cttcttaact ttctgagcac tttcaagcct
      361 aaccagttcc tggcgctttt gggcaatctg gcgtgcatat ttttctgatt caactacctg
      421 gaacggatca cctgccgatg gcactccgct gatcccaaga acttcaaccg gcattgaagg
      481 agtggcctcc tgtacctgtt caccggtatc agtgaacatt gcacgcaccc ttcctgaata
      541 tagaccaacc acaaacggat cacccacatg caatgtgcca ttctgaatca gcaccgttgc
      601 aataggacct cttcccgggt caagtttgga ctcaataaca acaccccttg cacggagttt
      661 aggattggcc ttaagctcaa gcatctctgc ctggattaat attaattcca aaagctcatg
      721 gatatttatt ttttgttttg cactaatttc agcaaaaagc gttgtgcctc cccactcttc
      781 aggtataagt tcgtatgcgg ttaactcctg tttcaccctg tttgggttag cttcaggaag
      841 gtctatttta tttattgcta ctataatagg cacatttgca gctttagcat gatttattgc
      901 ttctatagtc tgtggcataa caccatcatc tgctgctaca accagcacaa caatgtcggt
      961 tacacttgca ccacgcgcac gcattgtggt aaacgcttca tgccccggag tatcaagaaa
     1021 tgtaacatat ttatcttcaa cctttaccct gtatgcgcct atatgctgtg taatccctcc
     1081 atgttccgtt gcgacaacat tggtttcgcg tattgcatca agcaattttg ttttaccatg
     1141 gtctacatgt cccataacag taacaatagg tggccgggac acataatcct ggggccgatc
     1201 ctcttcatca actttgataa ctgtttcttc atataatgag accactttta cttcagtacc
     1261 atattcactt gccagtattg aagcagtatc agcatcaatg acctgattaa ttgtagccat
     1321 aacacccatt gacataagct tggcaattac ttcactggct ttgatattca tctttttagc
     1381 aagatcccca acggtaattg tttcggtaat ctctatttct tttggagcaa cagcttcccg
     1441 tggctgagat tctttttttc gcttcaggag caataatttt tcttccagtt cttctttttc
     1501 tttttttaag aaacgtttat ctttatccct gtctttttct ttggcttttc ctttttcctt
     1561 ttgccgtttc tgcaaatcca caacttcttt atcaataacc ggaacatctc ttttttcaac
     1621 tactgttggc gcatgctgtc tttcaggtct ttgttccttt tcttcagccc ctggcctttg
     1681 tttatcatct ttcctgaaat cttttttggg gaacctgtcc ttaggcttgc catgtctttc
     1741 ttttttatca aatcgctcct ttttttcctg cttttctttt tcaactactc ccgtctctgc
     1801 ccggtgtttc ggagcaactt cttttgaatc ctctttatgt tcaacaactt cttcagtagc
     1861 ttcgtcttct tttgcctcag cttctttttt taactctttt gcaacatgta cttttctttt
     1921 tagttttatt ttcttcttta attccgcttg ctgaataagt ttctttgctt cctgggctct
     1981 ctgttctttt attatctgaa tcttcttttc tataagaaag acatcgtcat ttgccagttc
     2041 agactgatca tctgtacagg ggatgccaag atccttacag atttttataa catcatctgt
     2101 tgaaacatga ttttttgttg ccacatcgat agctttcatt aacaaatccc tattagatta
     2161 aattattagc ctgttcttat gccatatcaa tggaagagag caacccggaa accctacctt
     2221 tataaatgat agactcttac ttccgttcaa atggcactat ctgtactatt cctgcttatc
     2281 ttcctgctca tcttcataat cttcttcttc aaagtcaaca tattcttgta ttatattaat
     2341 tatcttttga gctgtttttt caccaatacc atcaatattc atcagatcct ctacagattt
     2401 ttcaaccaga tcctcaacag agaaaattcc acccgcttca agcagagtaa tggttcgagc
     2461 atcaaggcct ggtaattctt ccagcggtgt ttcgccttct gatgttgttg caaatagctg
     2521 ctcaaccagt gcacgtgatt cacttgaact taagaattca cgatactggt cctcagtttt
     2581 aatgtcgata ttgaacccgc ataatttggc agccagccgc gcattgtgac cgtttttccc
     2641 gatagccaga cggtactgat cattgtcaac aaccgctata actccaccat ccctggtttc
     2701 aataatttct ttgacctttg ccggagtcag tgcatttgaa gccattacct ttttattggg
     2761 tgaatattct ataacatcaa tcttttcacc ttctagctca cggacaattg attgaatccg
     2821 tacacctttc atcccaacac atgcacccac gctatcaatg tcatccctgt cactggttac
     2881 ggctaccttg gtgcgcattc ccggctcccg tacaatattt actattttaa ctatgccatc
     2941 ataaatttcg ggaatttcca tttcaaaaag tttttcaaca aattttggat gagcacggga
     3001 aagtactaca ctgggacctt tgctgttttt ctgtacactc aacactatag ctttaactct
     3061 gtcgcctatt ttataatgtt caagtggcga ctgctcacgt gcaggaatta taccttcagt
     3121 ttttcctaaa tcaatataga gtgcttcttt tgttttacgc tggacatagc catttattaa
     3181 ttcatttact ttgtctttat actcatcata aacaatattt ttctctgcct ctttaatctt
     3241 ttgcaaaata acctgctttg ctgtttgtgc agctatacgc ccaaatgttt caagaggatt
     3301 ttcttccacg atgacctcat caccaaactt tgcatcaggt ttaatctttt gcgcttcttc
     3361 cagtgatatt tcttcagcat aatttttagg cacccgtacc accatttttc gcgaaataat
     3421 ctttacggta tttctttctc tatcaaaggt aatatctaca ggtttggtaa tgccgtattg
     3481 tttcttatat gcagacagca tcgctgactg aactatctcc tcaatcatct cacgggttat
     3541 ccctttttca gtagcaatct gatgcagtgc ttcaaaaaag tttccactca tactattcac
     3601 ctttaataat ctaaatttgc ttttatgatt tgagaatatg aaagagtcaa tactttattg
     3661 gtttcattaa tagtaactat ctccccctct atccctacaa gcgtgccttt gaatgttttc
     3721 ctggcatcat cctcctgata aatacctcta acaggcgctc cggtaaaccg tagaaagtct
     3781 tcaatagttc tgagttctcg ctttattccc ggtgaagaaa tttcaatagt atagtcatca
     3841 agcaactgtc cttcatctat acgatgggac aactccttgc tgaatttttc acaatcaaga
     3901 tgcgatatgc cattaatgtg atcaatttta acagtaatgc gcggtgtctt cccatgtaaa
     3961 acaagaacgc tgtcatatac actatagccc aggtcgtgag ccacattgtt aattattaac
     4021 tcaaggcttt cattgagttt cataataatg ctcatgttta ttgatatata acattgacta
     4081 aaaaaaagag tggcttttgc cacccgcttt acacacaatc gttaacaata aagcagaaaa
     4141 ccaactacct caattaatca atatagtatc atatgcttac aatttcaagc ttttttttaa
     4201 ctttacattc tctgcaggaa aaattctgat ttacatacta tgccatacta gtatatattt
     4261 tacctgcaat aaatcaataa atccctgtat ataaggtact gtatggaacg tgatattttg
     4321 caatcaatta aactggaaat tactaaaaat ctaaagttta caccatactt acgtatatgc
     4381 cttcatcctt ttattgcaca atcaaaaagt gatattgcac ataatatact atgcgcagaa
     4441 ctatctgcag agccagctat tcgattcagt gcaatacgta caataaccca ctataaactg
     4501 ccaggattca cagacctttt ccatgcttta ttccagcact caataaccga tgatgaaaag
     4561 atccagatct gcacatacct tgcaacatat gggaatagcc agacggtaga gcttttaaat
     4621 aattatatta tggataattt caataaagaa tcaaaccata caatagtaat acagtgcctt
     4681 gaaacgctga gaatattacg gcatcctgac agtaaactcc ttgctacatt aaaaagcata
     4741 attaatgaga caggaactca tgaggtaatc cggtactatg caatacgggc attgtcaatt
     4801 tacgatgata ttcatgtact gagttcatta attaatcaga atgaatatac tcttctggga
     4861 atttttgacg ccattgcatt tataagcgac tattgcatta ccaagaagac tcaaaaaaat
     4921 gaagcatcag attcatcaaa agaagaaaat cttattatag aactcagggt atttttatcc
     4981 aagatgctac ctcattttga tgaattttca acacgggtga aaatctcatg tttgaatgct
     5041 cttattacat ctaaacacag ggagaccaat gattatgtac taaaaatact tggtggccaa
     5101 aatgaaaatg aaaaagaaga actacttctt ttactccagc ataccatcat gttattacgg
     5161 gatccagaac cgttaatcag ggcactcatt tcatatggaa ctgtatcgcc ccatcataat
     5221 acaataattg ttgataccat tataaactat tttgaatcgt ttcagagcga cagaagcagt
     5281 acattattaa aagataaact ttttaattat ttcaccgtca cattggattc tttctttgaa
     5341 ctgtatagaa aaaattatat gatatcagat gtagaagaaa aaaattaccc cgaaatcttc
     5401 agggaggtaa ggaattttat actgttaaaa ttttctccac aaattttaaa cagaattatt
     5461 cattatctta agcatgagaa aaatgatgaa atacataaaa tcatcactct tttaaccact
     5521 tatttgcctt tcattgacag ctctattcga gatccatttt catcattggt tgaaatgctc
     5581 tatgacaggg atcccaaaag cagggaaatt actgcctcac ggttagaaac aattgacttc
     5641 gaaaagcgat ttttacagga acgaattatc aggttatgta atataatagc caaattaaat
     5701 attcaatcag ctgcaaccct ccttgtaaaa atctataatt atctaaaaaa gtatcgtgat
     5761 gagaaactat ttgatgcatg catacaaaca ttatccacca tgaaataccc ttatatgctt
     5821 ggtgaactgg aattaatgtt attatcaggc gaccgtaacg accagctttt ctcactcaaa
     5881 tacctggcac attatacaga ccagcaggca gcctctatcc tttttgaact tttgaagaat
     5941 acagcaaatc tggaccctga ggtgatagtt actgccttac atctcctgtt acaggacgaa
     6001 actgcacaat ataaaaattc atctgaaatc ctaaaaaaca ttgcactgac atataatgat
     6061 atagccatta agcaagccgc catacgtaat attggccact gtggcgatga aaaagagatg
     6121 gaatggctta taacactttt tactgaaaca aatgaaattt ctataaaaga aacaatatta
     6181 caatcaatta gcagcatcat accccgctta agagatttca ataaaagagc attgtctcag
     6241 ttcctcttag attgtttaaa agaatcaggg attcgcatcc gtatatatgc aatcgcaata
     6301 ttattacaac tcaacaataa ggaggttgca aaatatatta aagaaatgct aatcataaaa
     6361 aacagggaca tccagacaga aatgctatat atatttcata attacaatac accagaattt
     6421 tcatattttc tattatcctt attaaaggaa gaatatgcaa ttgctcctga aacagtatca
     6481 atattacaga atctacccgc tgaaatttct gaggacattg tcaattttat tggtaacatg
     6541 tacagaaaga atggaattga tatttctcag gccgaagcta ctttaacaat caaaccaggc
     6601 aaaattgata ctttaagcga tttctttata ataacaataa cgacatatgg agatacaaat
     6661 ccaattttac ttgaaaaact tgcgaccaca ctcaatacta tacaatccct tatcctgaat
     6721 cactgtaaaa aaaataacct tgtaatccat gcactgttgc ctggtttaat tacagtatat
     6781 tctaataatc ctttgaatat tgctgatgcc ctgatagcca tcgtacaaaa tatacagcag
     6841 tataatgata cttcacatat accatttaaa gcaattattc agtcctataa tgccagggta
     6901 atacaatcag ggcaggatat tataatagta tctgatgcac aatacgccca tgatatttta
     6961 tataaccatg ccatcataga tgaaaaactt tattctgcaa tacatgatga atttacctgc
     7021 aatcccctgc cgcacatact tgccaatcat gcacacattc cactgtttta tctatccaat
     7081 aaaaagaatt cgcttgtcga agcacat
//
LOCUS       DAOSZP010000030         7058 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_9020_length_7058_cov_3.445952, whole genome shotgun sequence.
ACCESSION   DAOSZP010000030 DAOSZP010000000
VERSION     DAOSZP010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 7058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7058
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9020_length_7058_cov_3.445952"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..705)
                     /locus_tag="PK348_01305"
     CDS             complement(<1..705)
                     /locus_tag="PK348_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004765954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HXK64880.1"
                     /translation="MTKVSNETVEKNNNNNRKNGNGNGDYAKKGNGTIQEGRLDLAEL
                     KSKTISSLMEIARSMGIEGVSGLKKQDLIFEILKAQTERSGLIFSSGVLEVLNDGYGF
                     LRSPNYSYLPGPDDIYVSPSQIRLFGLRTGDTVAGQIRPPKDNERFFALLRIEAVNYE
                     NPEKLQNRVLFDNLTPLYPETRINLETSPDEIDMRILNIIAPIGMGQRALIVAPPRTG
                     KTIILQKIANAITTNHP"
     gene            1373..1506
                     /locus_tag="PK348_01310"
                     /pseudo
     CDS             1373..1506
                     /locus_tag="PK348_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006189145.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(1508..2653)
                     /locus_tag="PK348_01315"
     CDS             complement(1508..2653)
                     /locus_tag="PK348_01315"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HXK64881.1"
                     /translation="MDLQSLFEGKRNGEFFYDTKGKRYIDCYCGAGTFNVGRHVQELS
                     NTLQEQADKSDQGNFVLISQEKALLASKLISFIPAKLNGVFFSAGRGEAMDFACKLAR
                     GFTGRTQLVTVDGGYYGETGFSVSLSDGDKKKSFGQIPDIVSIPFGNIQALEERITAK
                     TAAVITETIQVENACRSASEGFFKIMRTVCNRTGALMILDETQTGMGRTGKKFAFEHY
                     GVEPDILVIGEALGAGIFPIAATAFTRKIQKFMNQHPLIHLSTFGGHDLGCRVAYEAL
                     ELYEKTKPWENAGNVGQKLLEELAALQKQYPGKILSVHGRGLVIAITVRSDVMAQKAV
                     TKLVKEGIFTATGKVARDAVIIRPALTISEETAKTIARSIRRVFKTL"
     gene            complement(2655..4067)
                     /locus_tag="PK348_01320"
     CDS             complement(2655..4067)
                     /locus_tag="PK348_01320"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HXK64882.1"
                     /translation="MALWKDTLTDNDKKLIFSLFSRHISKGQTKTMRAAHLDFIETRR
                     KGIGFIDGLFGRKIIDCFTSAGCFNVGRGNPEIIKALNEALNTYDIGTNLLQSNVKKE
                     FIKKLASIAPGNCNQVIFASGGGDAIDCAIKLARGATGRKKIIAMKKAYHGHTGYSLS
                     ANGKDYYRHLFEPLEPNFVFVQFGDLDAIRQVAGIDTAAIILEPVQGEGGIFVGDDYY
                     LKSLRTICNQFSIMLIFDEIQTGFGRTGRMFASQHSGVVPDIMTLAKSISGGLSPNAV
                     VMYRNIPLLTQYVKSNPEFHQSITGGSDIGCAVGIAVIDYIVKNRLWENAESMGNIIM
                     EGLLSIQHENPDIIKEIRGRGLMIGVEYTHEYMGALMADSLARHGIFAVYSGNQPQVM
                     RFMVPITIHEQEVKKMLDIIRKAIATMRMYMPLTKPLSKIPLLKKLLDDIHVQIAVFD
                     IIRYFEDILGITKRNYSKEA"
     gene            4264..4863
                     /locus_tag="PK348_01325"
     CDS             4264..4863
                     /locus_tag="PK348_01325"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HXK64883.1"
                     /translation="MGRRILKDTRRGEIAQALYRCLLRKPFARTTIKDIAKEAKLNHG
                     MLHYYFKNKEEILLYFLDWIVALHVDDFKRWAQKQKLESRNFAEALSLALDYAKNKIT
                     LNEELAKIFIEVWEIALYHKKVRTKLQYVYKEWIEQLVAIIGKEITKKIATTLSVATI
                     AFLEGISLFHIMLSDIYPIKDILNSFEKKIESVINKRNI"
     gene            complement(4898..5905)
                     /locus_tag="PK348_01330"
     CDS             complement(4898..5905)
                     /locus_tag="PK348_01330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HXK64884.1"
                     /translation="MKQWLYKHSIYCILAMIFVPVAIAQSYPKISAYMPSYALYTESG
                     QNKTTRYIIIRRLKYNSVQAYVYINPDTLHTGILKNPKGTLQQNSLQNIIQQCADTQY
                     AKLYQNALQKSKRMFNAGITHVDTHGMILSFDLCPSKRFLDTDCIEQLIALNKIVPLP
                     VAICISGKWMLTHPEDLQWLQSLASAHQLDITWVNHSFTHFYSRKLPVRKNFMLKEDT
                     SVAREVLQNEICMLEHDIFPSIFFRFPGLISNQKIYEKVMGYGLIPLGADAWLAKGKR
                     PVTGSIILVHPNGNEKKGTYILIQLARSGLINITNIVSLTAACTNSSQTASIHKKQVK
                     L"
     gene            complement(6086..6694)
                     /locus_tag="PK348_01335"
     CDS             complement(6086..6694)
                     /locus_tag="PK348_01335"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HXK64885.1"
                     /translation="MKRVAKKNSRGQSRKPPSPLPEKLKQRLYPAVLELFADNDFHHV
                     NIREIYRKSGISPSTIYRYFPSKEDLLFTILDEKISEIGVLVREHIKGIESTKEILRK
                     IFWVTMDYYDRNPGVAVTAFITVPMRTWMKERSYRRDDASAIIEEVVKRARDRGDIDP
                     SIRTSQILDQYYMYCYRQIHLWYFNGMTTRLVDRIPAFFDIL"
     gene            complement(6767..>7058)
                     /locus_tag="PK348_01340"
     CDS             complement(6767..>7058)
                     /locus_tag="PK348_01340"
                     /inference="COORDINATES: protein motif:HMM:NF013722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HXK64886.1"
                     /translation="HTSVGFARIVGLPDIILQGTATLAFAARELTDNEAAGNPQRLKE
                     LSCRFTGMVVPPADIRVLLLQREEHASGTDLFFEVHGKEGRVLSNGYARISG"
BASE COUNT         2096 a         1374 c         1364 g         2224 t
ORIGIN      
        1 agggtgattt gtggtgatag cattagctat cttctgtaag attatggttt taccggtacg
       61 gggaggagca acaatcagtg cgcgctgtcc catccctatg ggtgcaatta tgttaagaat
      121 gcgcatatct atttcatcgg gtgatgtttc caagtttatg cgtgtttcag gatacagtgg
      181 tgtgaggtta tcaaataata cacggttttg cagtttttct gggttttcat aattgaccgc
      241 ttcaatacga aggagggcaa aaaagcgctc attatccttg ggtggccgaa tttgccctgc
      301 aacggtatcc cctgtccgta agccaaaaag ccgtatctgt gaaggcgata catagatatc
      361 atcagggccg ggcagataac tgtagttggg tgagcgaagg aacccgtatc catcgttaag
      421 aacttccagt acaccgcttg agaagattaa accgctgcgc tcagtttgag cttttaatat
      481 ttcgaatata agatcctgtt ttttaagtcc tgaaactccc tctattccca tggaacgtgc
      541 aatttccata agggaactga tggtttttga tttgagttca gctaaatcta aacggccttc
      601 ctgaatggtt ccattgcctt tttttgcata atctccgttt ccgttcccgt tttttctgtt
      661 attattgttg ttcttttcaa cggtttcatt tgatactttt gtcatagcac acctttagaa
      721 tatttaagtt tggaatttat atgaacagct tctttaaaaa caagaattat ctctgctata
      781 caataactgg ttaaaccgta tggattgtgt aaaatttaca cgaaaatgta tggtaatagt
      841 ttaaaataac aaataacatt atgttaacga tattgaacag taccttatta ctttattgct
      901 aagaggattt tcccatgatg atttattttt gctgtaatta tttaatacat atatcataat
      961 ctacaaaagg ttatataaaa actctttcag caggaaggca actcgtttaa catatgttct
     1021 tttcataaaa tatactataa aaaatcattt atgtcacaaa aaaatactat ctttttttat
     1081 tttttacaag agtacatcat actcaaattt cgtcaagaaa aaatgcaaaa ttaaatggtg
     1141 tttatcttca tgaattcttg ctattacttt atacggaatt tatagtcaat aacttcccaa
     1201 aaattttttg taaaatataa catattacaa taccgatctt atataatccc atagttcaat
     1261 attgtaactt acaaagtgta atttgtatta acaatttgca agttatatag tattcaataa
     1321 taactgatgt gcatatatta ttacctatcc atagtgttac aaaagaaacc atatgataaa
     1381 acgtgaagct gaacaaacat taaaaaatta tcaaaacagt tccctgtggt agcaatcaca
     1441 ggtcctcgcc agtcaggaaa gaccactctg gcgcaatatg tatttaacaa taaaaactat
     1501 gtttcatcta taatgtttta aatactctac gtatactccg tgcaattgtc tttgctgtct
     1561 cttcggaaat tgttaacgcc ggtcgtatga tgactgcatc gcgagccact ttgccagttg
     1621 ctgtaaaaat accttctttc acaagttttg taacggcttt ctgtgccatc acatcactgc
     1681 gtactgtaat agcaataaca agtcctctac catgtactga taaaattttt ccaggatatt
     1741 gtttttgtaa ggcagcaagt tcttccagga gcttttgccc aacattccct gcattttccc
     1801 atggctttgt tttttcatat agttcaagtg cttcataagc aaccctgcat ccaagatcat
     1861 gtccaccaaa cgttgacaaa tggataagtg gatgctgatt catgaacttc tgtatcttcc
     1921 tggtaaatgc tgtggctgct atggggaata tgccagcacc cagtgcttca ccaataacaa
     1981 gaatgtcagg ttctacacca taatgttcaa aagcaaattt tttgccggtg cggcccatgc
     2041 cggtttgcgt ctcatcaagt atcataagtg cgcctgtcct gttgcacact gtgcgcataa
     2101 tcttaaaaaa gccttcactg gcactgcggc atgcattttc tacctgtata gtttcggtga
     2161 taacggccgc agtttttgct gttattcttt cttcaagtgc ctgtatatta ccaaatggta
     2221 tggagacaat atcgggtatc tgcccaaaac ttttcttttt atcgccatcc gacagtgata
     2281 cggaaaaacc ggtttcaccg taatagccac catcaacggt taccagttgt gttcggccgg
     2341 taaatccgcg agccagttta caggcaaagt ccattgcctc accacggcct gctgaaaaga
     2401 atacgccatt gagtttggct ggaatgaatg aaataagttt tgaagctaaa agcgcctttt
     2461 cctgtgatat taaaacaaaa ttaccctgat cgcttttatc tgcctgctct tgtaatgtgt
     2521 tcgatagttc ctgaacatgt cgacctacgt tgaacgtgcc agcaccacag tagcagtcga
     2581 tatatcgctt gccttttgta tcatagaaaa attcaccatt tcgttttcct tcaaataatg
     2641 attgcaaatc catgttatgc ctccttactg tagtttcttt ttgttatgcc aaggatatcc
     2701 tcaaagtagc ggataatatc aaacacagct atctgtacat ggatatcgtc taacagtttt
     2761 ttgagcagtg gaatttttga taatggtttg gtaagcggca tatacattct catggtagct
     2821 atcgctttgc gtataatatc taacatcttt tttacttcct gttcatgtat ggtgatggga
     2881 accatgaagc gcattacctg tggctgattg cctgagtata cagcaaagat gccatgccgt
     2941 gccagtgaat cggccataag cgctcccatg tattcatgag tatattcaac cccaatcata
     3001 agtcctctgc cccggatttc ttttatgatg tcaggatttt catgctgaat ggaaagaagt
     3061 ccttccataa tgatgttacc cattgattct gcattttccc acaggcggtt ttttacaata
     3121 tagtcgatca cggcaatgcc tacggcacac ccaatgtcac ttccaccggt tattgattga
     3181 tgaaattccg gattgctttt aacatactgt gtaagaagcg gaatgttgcg gtacatcacc
     3241 acagcattgg gggaaaggcc gccacttata gatttggcaa gggtcatgat atcgggcaca
     3301 acaccactgt gctggctggc aaacatcctg cctgtgcgcc caaaacctgt ctggatttca
     3361 tcaaagatga gcatgatgct aaactggtta catatagttc gtaaactttt caggtaatag
     3421 tcatcaccca caaagatgcc accttcaccc tgaactggtt ccagtataat ggcggcagta
     3481 tcaatgccgg caacctgccg tattgcatct aagtcgccaa attgcacaaa gacaaaattg
     3541 ggttcaagtg gttcaaaaag gtggcgatag taatccttac catttgctga aagtgaatac
     3601 cccgtgtggc catggtatgc ttttttcatt gctatgattt tctttcttcc cgttgcgcca
     3661 cgagctaact ttattgcaca gtcaatcgca tcaccgccac cagaagcaaa tataacctga
     3721 ttgcaatttc ctggagcaat tgatgctaat ttttttataa attctttttt aacgtttgat
     3781 tgcaaaaggt ttgtgccaat atcatacgtg ttcagcgctt cgttaagtgc tttgataatt
     3841 tcaggatttc ctctccccac gttgaagcag ccagctgatg tgaagcaatc aatgatcttt
     3901 cgcccaaata gtccatcaat aaaaccaatg cctttgcgcc gggtctcaat aaaatctaaa
     3961 tgtgcagcac gcattgtctt ggtttgccct ttagaaatgt gccgtgaaaa tagtgaaaag
     4021 ataagttttt tatcattatc agtgagcgta tctttccata aagccatacg aacctccttt
     4081 tagtatatat tagttggatg actaacataa aatatttatt ttgtcaataa attaatattg
     4141 actggctggc aattattttt gaacaatgga gtattgcaaa aaaaagtgta cactcatgtc
     4201 acttgcgcct gtgtgtacac tatctggaag tgataaaaaa tataaaatgg aaatcatggt
     4261 gtaatgggca gacgcatttt aaaagacaca cgcagaggag agatagcaca ggcgttatat
     4321 cgctgcctgt tacgaaagcc atttgctcga acaacaataa aggatatagc aaaagaagcc
     4381 aaacttaacc acgggatgct acattactat tttaaaaata aagaagaaat attactgtat
     4441 tttttagact ggattgttgc attgcatgta gatgatttta aaagatgggc gcaaaaacaa
     4501 aagcttgagt cacgcaattt tgctgaagcg ctgtcactgg cgctggatta tgcaaagaat
     4561 aaaattacat tgaatgaaga actggcaaag atattcattg aagtgtggga aatagcactg
     4621 tatcataaaa aagtacgaac aaaactacag tatgtatata aagaatggat tgagcaactt
     4681 gttgctatca taggcaagga aataactaaa aaaattgcaa caacgctgtc agttgcaacc
     4741 atagcttttc ttgaaggcat ttcactgttt catataatgc tgtctgacat atatcccatt
     4801 aaggatatac tgaattcgtt tgagaaaaag attgaatcag tgataaacaa aagaaatata
     4861 tagtgacaaa agtttctggt agcaatggtt cgtattatta cagcttaacc tgttttttat
     4921 gtattgaagc agtttgtgag ctatttgtac atgctgctgt caatgataca atatttgtta
     4981 tattaattaa ccctgaacgg gcaagttgga tcaaaatgta ggtgcctttc ttttcattac
     5041 cattgggatg taccagaata atactgccag tcacaggcct tttgcctttt gcaagccatg
     5101 catctgcacc taaagggatg agtccatacc ccattacctt ttcatagatt ttttgattgg
     5161 aaataagccc gggaaatctg aaaaaaattg aaggaaaaat atcatgttcc agcatgcaga
     5221 tctcgttctg aagtacttcg cgtgccacac tggtatcttc tttcagcata aagttttttc
     5281 gcactggcag cttccgcgaa taaaaatgag taaagctgtg atttacccag gtgatgtcaa
     5341 gctggtgagc agaagctaaa ctctgtaacc attgaagatc ttctggatgg gtgagcatcc
     5401 atttcccgct gatgcatatt gcaacaggta gtggtacaat tttgttcagt gctatcagct
     5461 gttcaataca atcagtatca agaaagcgtt ttgatggaca caggtcaaag cttaaaatca
     5521 tcccatgtgt atcaacatgg gttatacctg cgttgaacat acgttttgat ttttgcaatg
     5581 cattttgata cagctttgca tattgtgtat cagcgcattg ttgaataata ttctgaagag
     5641 aattttgttg caaagtgcct ttaggatttt taagtatacc agtgtgcagt gtatctggat
     5701 ttatatatac gtaagcctgt acggaattat attttaatct tcgtataatg atgtaacgag
     5761 tggttttgtt ttgccctgat tcagtataca gtgcataaga gggcatatat gcactaattt
     5821 tgggatatga ttgggcgata gctaccggaa caaaaatcat tgccagtata caataaatac
     5881 tatgtttgta aagccattgt ttcattataa aaccttacag tatgtgcgat agttaccgga
     5941 atcaagcttt ctctatgaaa ggaagtaaat gaacaaaacc cgtataaaag tcaattaaat
     6001 aatttttata aaaaatgaca attatactgt ggtattttag tactgtccat ttttacgatc
     6061 catgctcagg tgggctgagt gtttttcaca gaatatcaaa aaaagcagga atgcggtcaa
     6121 caagccttgt tgtcatgcca ttgaaatacc ataaatgtat ctgtcggtag caatacatat
     6181 aatactgatc aaggatctgc gaagtccgta ttgaggggtc aatatcacca cgatcacgag
     6241 cacgtttgac tacttcttca ataatagctg aagcatcatc ccggcgatag gatcgttcct
     6301 tcatccaggt acgcatggga acggtaatga aggccgtgac agcaacacca ggattgcggt
     6361 catagtagtc cattgtcacc cagaaaattt ttctgaggat ttcctttgtg ctttcaatac
     6421 ccttgatatg ttctcgtact aaaacgccaa tctcggaaat tttttcatcc agaatggtaa
     6481 atagcaaatc ctcttttgat ggaaaatatc ggtaaatggt gcttgggcta atacctgact
     6541 tccggtaaat ttcacggata ttgacgtggt ggaaatcatt atcagcaaag agttcaagaa
     6601 cagcgggata aagccgctgc ttgagttttt caggtaaagg cgaagggggc ttgcgtgact
     6661 gcccccgtga atttttttta gcaactcttt tcatagctgc tcactctttg cctcttgaga
     6721 aaaaactcaa tatttttttt atgcagtgtc agatttttta gaaagttcaa ccacttattc
     6781 gtgcatagcc gtttgaaaga acgcgacctt cttttccgtg tacttcaaag aaaaggtcag
     6841 tcccactggc atgctcctcc cgctgtaaaa gcagtacccg gatgtctgca ggtggtacca
     6901 ccatgcccgt aaatctacag gaaagttcct tcaaacgttg tggattgccc gcagcttcat
     6961 tatcagtaag ttctcgtgca gcaaatgcaa gcgttgctgt gccctggaga atgatatctg
     7021 gcagcccaac aattcgggca aagccaacag atgtgtgg
//
LOCUS       DAOSZP010000031         7021 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_9089_length_7021_cov_4.244760, whole genome shotgun sequence.
ACCESSION   DAOSZP010000031 DAOSZP010000000
VERSION     DAOSZP010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 7021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7021
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9089_length_7021_cov_4.244760"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            94..1818
                     /locus_tag="PK348_01345"
     CDS             94..1818
                     /locus_tag="PK348_01345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK64887.1"
                     /translation="MMNVIAYEQSIRGHYDYLKDLKGNTSTLFSELIQRFTVINMRID
                     EFFTMIRPYIYYFYNTDENLPDTFLHDVIIGTNNEMNEYIDTIVKYMIADRETNKEVE
                     NAINKTLTLSNSVAMILDAVEAIDLHAKNTMIISIKAGEEGSTLTTIAVEMSHLADMV
                     NDVSSQFQEIIEYLDKLRTKFSNTCQTVDIIIENYLTHLQIKMNSTIREIIGHQKKLS
                     YNVESIVSFADQLKSSIHKILQEFQVEDIFRQDIEKILFAIEALEEGGIDDSEKDILF
                     WGMYQKLLSLQHDFMHLYTSAGESLHTMKSNVEAIASDYTKASETVFEGDEFAIEKVY
                     NSLEMLQHETIQYIEDILAKKASLLEISSDVLEQLHKFQEFFTKIQDVVRKFDVVNML
                     TRIELARHQQLQKTIASSLSDISIMPKKIKRIVEESESLYKDVIGTMESTYQSYKEAM
                     AIQDTILQNCVSNLKKVSLKLYESKKYYIDISQQIERNVNTLQQFLSEKSEQIEAFKN
                     IEENIKLFCDMLDGAGVEKNMPDVEKINKAVDTLTMLYGTDYKGLMLVSLLKEYSAAK
                     TEDTVIVF"
     gene            1985..2239
                     /locus_tag="PK348_01350"
     CDS             1985..2239
                     /locus_tag="PK348_01350"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HXK64888.1"
                     /translation="MYIIEVDEFVNVKHIKQFADEVITVLRTEKDIVIDFTKSKRLDL
                     SVVQVILSLLKTARQLNKTVKFKGVNDTVKKQLKLCGVIR"
     gene            2359..2730
                     /locus_tag="PK348_01355"
     CDS             2359..2730
                     /locus_tag="PK348_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006317662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXK64889.1"
                     /translation="MKHIVIIDDSPTIRTSVEYTLKDSGYGMVHAENGVDALNKIKDL
                     LSKGDEIALCIVDINMPQMDGITFIKKFRENDKFTPVVVLTTEAEEEKIQEGKKAGAS
                     GWMIKPFKAEQLQSVVHKFLR"
     gene            2741..3232
                     /locus_tag="PK348_01360"
     CDS             2741..3232
                     /locus_tag="PK348_01360"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HXK64890.1"
                     /translation="MNIENTAIENKPDTASTEQFVTFIIGDETYGIEVLKVQEIIGMT
                     HITHVPNTLSFMKGVINLRGSVVPVIDMRKKIGMEEIEYNAFTVIIITEVKGKLIGMI
                     VDSVQDVVTIPVQKIQDTMRFTSHVSTDYIKGIGQIDDNLVIILDVDKLLTSEELAHI
                     KTT"
     gene            3257..3820
                     /locus_tag="PK348_01365"
     CDS             3257..3820
                     /locus_tag="PK348_01365"
                     /inference="COORDINATES: protein motif:HMM:NF015908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK64891.1"
                     /translation="MIRVFDATIKQPVVILVPGEYFAAENQVLSTIVGSCFVVCLYDS
                     IKKIGGMGHCLLPVLSSLQMKKEDIFNYNINFMEHIIGQMVKLGADRKNFIVKVFGGT
                     GSSYNQKFTTDFISEYCKNENMSLDAIDIGGNYRRKLYFFCDTGKVHRYLVEANDSVS
                     EFIKMEKEFIDKVLTDKVQYGNVILFE"
     gene            3824..4636
                     /locus_tag="PK348_01370"
     CDS             3824..4636
                     /locus_tag="PK348_01370"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HXK64892.1"
                     /translation="MNNPFYQQLDQQDFDYIRNIVYRESGIKLSEMKRALVQSRLLRR
                     MRELGMQRYDEYCDFLRENYDDEIENLINCITTNKTEFFREREHFSILQRIVEQELSH
                     KNISIWSAGCSTGEEAYSIAIVLCESNFKKQFTIIATDIDTKVLETARSGIYPLEVVQ
                     TLDISLLKKYFLKGKGQYDGYAKVKNFLKEKISFQHLNLLDDNYRLKGHFDVIFCRNV
                     MIYFDKDVQVKVLKKFYDYLPKGGYLFLGYAEAIVSANLPFHYVAHSVYQKR"
     gene            4672..5238
                     /locus_tag="PK348_01375"
     CDS             4672..5238
                     /locus_tag="PK348_01375"
                     /inference="COORDINATES: protein motif:HMM:NF015908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HXK64893.1"
                     /translation="MYIRNSHRLKKPMKVLYPGDCYVSNKDEYIGTLLGSCVAVCLYD
                     PVNKVAGMNHFVLPGKVVQAHTKQKKSNAGDEEKELLKYGTRSIEKLITQMEQYGKRE
                     NIVAKIFGGGKVLDYQTTQYGISNMNVRIAKILLEMADIPIVSEDVGGTVARKVMFDV
                     ATGRAYCKQLTKIEEKDKTFTMELSQFE"
     gene            5231..6298
                     /locus_tag="PK348_01380"
     CDS             5231..6298
                     /locus_tag="PK348_01380"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010662143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HXK64894.1"
                     /translation="MNKIKVLVVDDSAVMRKTLTDILNGSSKIEVVGTALDPYIAVNK
                     IKQLNPDVLTLDIEMPRMDGLTFLRKLMLANPMPVIMVSAFTDKGALQTIQALEYGAV
                     DFILKPRSDDSAEWDRFASELREKVITAASIQSDRFTARSREKAIEVEEKFSADVILP
                     KTVSKVRTTHSDVIIALGASTGGTEVIAKILGSLHENVPAIAIVQHMPEKFTEAFANR
                     VNNNSKLYVKEAQNGDRLYRGTALIAPGNRHMLVRSDASGYYVEINDGPPVNRHKPSV
                     DVLFRSVAQNVSKSIGILCTGMGADGAEGLGEIMQAGGMTIAQDEASSVVFGMPREAI
                     KRGAAQKVMNIEQMIAYINSL"
     gene            complement(6414..6704)
                     /locus_tag="PK348_01385"
     CDS             complement(6414..6704)
                     /locus_tag="PK348_01385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HXK64895.1"
                     /translation="MVTVENNTITIHCEKATPDDKVEVEYAVERLLGQGVRVIYFDLS
                     KTIYLPSTLMGYLMWKKQDLQKQGKDIQIIAISQSLRKVFEETRLIEFFGIT"
     gene            complement(6711..>7021)
                     /locus_tag="PK348_01390"
     CDS             complement(6711..>7021)
                     /locus_tag="PK348_01390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK64896.1"
                     /translation="IIFQKNINGTDYVGIAFGENPKTNRAFKVYISWQSSNISDYNGT
                     ASVTVIDTDGIKYSNETATIDFDISGPSSNVYNITSSDDITIGTKTLTITSINAYLVQ
                     "
BASE COUNT         2387 a         1006 c         1451 g         2177 t
ORIGIN      
        1 ggtgccgcaa tattaggtga tggaactatc gcccttatta ttgatgtaca tggttttata
       61 agttcgtaat gcatagttca ggactaacac gatatgatga atgtaattgc ctatgaacaa
      121 tccatcagag gtcattatga ttaccttaaa gacttaaaag gtaataccag tactctattt
      181 tctgaactga tacagcgctt tactgtaatt aatatgcgga ttgatgaatt cttcaccatg
      241 attcgtccct atatttatta tttttataac actgatgaaa accttcctga tacttttttg
      301 catgatgtca taattggtac caacaatgaa atgaatgagt atattgatac cattgttaaa
      361 tatatgatag ctgacagaga aaccaataag gaagtggaga atgcaattaa taaaactctt
      421 actttgagta acagcgttgc tatgatactg gatgcagtgg aagcaattga tttacatgca
      481 aaaaacacca tgataatttc cattaaggct ggcgaagaag gctctacgct tacaaccatt
      541 gcagtggaga tgtcacattt agctgatatg gttaatgatg tttcaagtca atttcaggaa
      601 attatagaat atttggataa attacgcaca aaatttagca acacctgtca gacagttgac
      661 attattatag aaaactatct cacacatttg cagattaaga tgaacagcac aatacgtgaa
      721 attattggcc atcaaaagaa gctgtcatat aatgttgagt caattgttag ttttgctgat
      781 cagttaaaaa gcagtattca caaaatatta caggaatttc aggttgagga tatattccgc
      841 caggatattg aaaaaatatt atttgctatt gaagcgctgg aagaaggtgg tattgatgac
      901 agtgaaaagg atatcctttt ttgggggatg tatcaaaagt tactgtcgct tcagcacgac
      961 tttatgcatt tatataccag tgcaggggaa agcctgcata caatgaaaag taatgttgag
     1021 gctatagcaa gcgattatac aaaagcttct gaaacagtgt ttgaaggtga tgagtttgct
     1081 attgaaaaag tatataattc actggaaatg ttacaacatg aaactattca gtacatagaa
     1141 gatattcttg ccaagaaagc ttcattgctt gaaatatcca gtgatgtatt ggagcaatta
     1201 cataagtttc aggagttttt tactaaaata caggatgtgg tgcggaagtt tgatgtggtg
     1261 aatatgttaa cacggataga acttgcacgg catcagcagt tgcagaaaac catagccagt
     1321 tcgctcagtg atataagcat tatgccaaag aaaattaaga ggatagtaga agaatcagaa
     1381 agtttataca aggatgtcat tggcaccatg gaatctacct atcagtctta taaagaggcc
     1441 atggctatac aggatactat attgcaaaat tgtgtatcca atctgaaaaa ggtttcatta
     1501 aaattatatg aatccaagaa atattacatt gatatatcac agcagataga acggaatgta
     1561 aatactttgc agcaattctt aagcgaaaaa tcagaacaga tagaagcatt caaaaatatt
     1621 gaagagaata taaagctgtt ttgcgatatg cttgatggtg caggagttga aaaaaatatg
     1681 cctgatgttg aaaagattaa taaagccgtt gatacactta caatgctata tggcacagat
     1741 tacaaagggt tgatgcttgt ttcactttta aaagaatatt cggctgcaaa aacagaggac
     1801 acggttatag ttttttaatt aatgtgcaac aggggtattg cataaggttc agattgatag
     1861 aattatgaaa gttgaagagt catactggaa tatatgccaa acttattttc aagtaggtaa
     1921 tagtgcgatg ttaaaccaga ttcagtatga cagtgatgat gtcagtgcat aaagggggca
     1981 atacatgtat ataatagaag ttgatgaatt tgtaaatgtt aaacacataa agcagtttgc
     2041 agatgaggtg ataacagtat tgcggactga aaaagatatt gtcattgatt ttactaaatc
     2101 aaaacggtta gatttgtcgg ttgtgcaggt tattttatca ttattgaaaa cagcccggca
     2161 attgaataaa acagttaaat ttaagggagt taatgatacc gtaaaaaagc aattaaagct
     2221 atgtggcgtt atacgttaat ttttgttgtt aagtattatg aattgtatat tattatagca
     2281 cgggggaata ataaagctgt tctggctaat taataggaat gcaaagtcaa atacaatctt
     2341 ttttaagagg agctttatat gaagcatatt gtaatcattg atgactcgcc aactatacgt
     2401 acaagtgttg aatatacact caaagattca ggatatggta tggttcatgc tgaaaatggt
     2461 gttgatgcat tgaataaaat aaaggattta ctttctaaag gggatgaaat tgccctgtgt
     2521 attgtggata ttaatatgcc acagatggat ggtattacgt ttattaaaaa gttcagggaa
     2581 aatgataaat ttacaccggt tgtggtgctt actacagaag ctgaagaaga aaaaatacag
     2641 gaaggcaaaa aagcaggtgc ttcagggtgg atgattaagc catttaaggc tgagcagctt
     2701 cagtcagtgg tccataaatt tttgcgataa gggggaatat atgaatattg aaaatacagc
     2761 tatagaaaac aagcctgata ctgcttcaac agaacaattt gttaccttca ttattgggga
     2821 tgaaacctat ggtatagaag ttcttaaagt gcaggaaatt attgggatga cccacattac
     2881 tcatgttcct aatacacttt catttatgaa aggtgtcatt aacctcagag gttcggtggt
     2941 gccggtaata gatatgcgga aaaaaatagg gatggaggag atagaatata atgccttcac
     3001 tgttataatt attactgagg ttaaagggaa acttataggg atgatagttg attcagttca
     3061 ggatgtggtt actattccag tgcagaaaat tcaggatacc atgcgattta catcgcatgt
     3121 gtcaacagat tatattaagg ggattggaca gatcgacgac aatttagtca ttattctgga
     3181 tgtcgataag ctgttaacaa gtgaggaatt ggctcatatt aaaacaacat aaattaatgc
     3241 aaacaggtat gatgggatga tacgggtatt tgatgcaacc attaaacagc cagtggtaat
     3301 tctggtgcct ggtgaatatt ttgctgcaga aaatcaggtt ctttctacga tagttggtag
     3361 ttgttttgtt gtatgcctgt atgactctat aaaaaaaatt ggtggaatgg ggcactgttt
     3421 attgccggta ttatcttcat tacagatgaa aaaagaggat atattcaatt ataatattaa
     3481 ttttatggag catattattg gccagatggt caagttaggg gctgatagaa agaattttat
     3541 tgtaaaggta tttggtggga cagggtcatc atacaatcaa aaatttacta ccgattttat
     3601 ttctgagtac tgtaaaaatg aaaatatgtc tcttgatgcg attgatattg ggggtaacta
     3661 tcgccgtaaa ctttattttt tttgtgacac aggtaaagtt caccggtatt tagttgaagc
     3721 caatgactca gtttcagaat ttataaaaat ggaaaaggaa tttattgata aagtattaac
     3781 tgataaagtt caatatggca atgttatatt atttgaataa tatatgaata acccttttta
     3841 ccaacaatta gaccagcaag attttgatta catcagaaat attgtgtata gagaatcagg
     3901 cataaagctt tctgaaatga aacgtgcact ggtgcaatcg cgtttgctac gcagaatgcg
     3961 tgagttaggt atgcaacgct atgatgagta ttgtgacttc ctcagagaaa attatgatga
     4021 tgaaatagaa aaccttataa attgcatcac taccaataaa acagagtttt tcagggaaag
     4081 agaacatttt tctatattgc aacgaattgt tgaacaggaa ctatcgcata aaaatatttc
     4141 aatatggagt gctggctgct caaccggtga agaagcatac agtattgcta ttgttttatg
     4201 tgaaagtaac ttcaagaaac aatttactat aattgccacc gatattgaca caaaagtgtt
     4261 agaaacagca cgaagtggca tatacccact ggaggttgta caaacacttg atataagtct
     4321 attaaaaaaa tattttttaa aaggcaaagg ccagtatgat ggatatgcaa aagtaaaaaa
     4381 ttttctcaaa gaaaaaattt catttcaaca cctgaattta cttgatgata actatcgcct
     4441 taaagggcat tttgatgtta tattttgccg caacgtcatg atttattttg ataaagatgt
     4501 tcaggtcaaa gtattaaaaa aattttatga ttatttacca aaaggtggct atcttttctt
     4561 aggatatgct gaagcgatag taagcgccaa tcttcctttt cattatgtag cccattcggt
     4621 atatcaaaaa cgataataca taatgctgtg ttagtgttaa tagaggtagc catgtatatt
     4681 cgcaattcac acaggttaaa gaagccaatg aaagttttat atcctggtga ctgttatgtt
     4741 agcaataaag atgaatatat tggaacgttg cttgggtcgt gtgtagcagt atgcctgtat
     4801 gatcctgtaa ataaggtggc cgggatgaat cattttgttt tgccgggaaa ggttgtgcag
     4861 gctcatacaa agcagaaaaa atcaaatgct ggtgatgaag aaaaagaact gttaaaatat
     4921 ggcacaaggt caatcgaaaa gctcattact cagatggaac aatatggtaa aagagaaaat
     4981 atagtagcta agatttttgg tggaggaaaa gttttagatt atcaaaccac acaatatggc
     5041 atttccaata tgaatgtaag aattgcaaag attttactgg aaatggccga tattcctatt
     5101 gtaagcgaag atgttggtgg caccgttgct cgtaaagtta tgtttgatgt tgcaacagga
     5161 agagcatatt gtaagcaatt aacaaagatt gaagaaaaag ataaaacttt cactatggag
     5221 ttatctcagt ttgaataaaa taaaagtttt agtggttgat gattctgcgg taatgcgcaa
     5281 aacattaacc gatattctta atggttccag taaaattgaa gttgttggta ctgctcttga
     5341 cccttatatt gctgttaata aaataaagca gctcaatcct gatgtattaa cccttgatat
     5401 tgaaatgccg cgcatggatg gccttacttt tttgcgaaag ctcatgttag caaacccaat
     5461 gccggtaatt atggtcagcg cattcactga taaaggtgcg ttgcaaacaa tacaggcgct
     5521 tgaatatggt gctgttgatt ttattttaaa gccacgttca gatgatagtg cagaatggga
     5581 cagatttgca agtgaattgc gtgaaaaggt tatcaccgct gcaagcatac aatcagatag
     5641 atttacagca cgtagtagag aaaaggctat tgaggttgaa gaaaaattta gtgctgatgt
     5701 aattttgcca aaaacagtat caaaggtgcg gaccacccac agtgatgtta tcattgctct
     5761 gggtgcatca acaggtggaa ccgaggttat tgcaaaaatt ttgggtagcc ttcatgaaaa
     5821 tgtaccggct attgctattg tacagcatat gcctgagaaa tttactgaag catttgccaa
     5881 cagggttaac aacaactcaa agctgtatgt aaaagaagct cagaatggtg acaggctgta
     5941 caggggcaca gccctcattg caccaggaaa ccgtcacatg ctggtgcggt ctgatgccag
     6001 tggatattac gttgaaatta atgatggacc accggtaaac cggcataaac catcagtgga
     6061 tgtgttattt cgttcagtag cacagaatgt gagtaaaagt atagggatat tatgtaccgg
     6121 tatgggtgct gatggggctg aaggacttgg tgaaattatg caggcaggtg gcatgacaat
     6181 agctcaggat gaagcttcgt cagtagtgtt tggtatgccg cgtgaggcta tcaagcgagg
     6241 tgcggcacaa aaagtgatga atattgaaca gatgattgcg tatataaata gtctatgata
     6301 tatatactct tttcttttta aaatatgtct ttaaatagat aggcttatcc aatactttac
     6361 atcctgacca gcttcagcat tttcccctat cagttaaaaa ataattaatt ttattacgtt
     6421 attccaaaaa attcaattaa ccgtgtttct tcaaatactt tacgcaatga ttgacttatt
     6481 gctattattt gtatatcttt gccctgtttt tgcagatcct gctttttcca catgaggtag
     6541 cccataaggg ttgatggcag gtaaatggtc tttgataaat caaaataaat aactctaaca
     6601 ccttggccaa gcaaacgctc aacggcatat tccacttcca ctttgtcatc aggggtagct
     6661 ttttcacagt gtattgtaat ggtgttgttt tctacggtta ccatacagtg ttattgtacc
     6721 aggtatgcgt ttattgaagt aatggtaagt gtttttgttc cgattgttat atcatctgaa
     6781 ctggtaatat tataaacatt tgaagatgga ccagatatgt cgaaatctat cgtagcagtt
     6841 tcattagaat atttaattcc atcagtatct attactgtta ctgatgctgt accattataa
     6901 tcacttatat tagatgattg ccagctaata taaaccttaa aagctctatt agttttagga
     6961 ttttcaccaa aagctatacc aacataatca gttccgttaa tatttttttg aaaaataatt
     7021 g
//
LOCUS       DAOSZP010000032         6908 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_9320_length_6908_cov_3.510142, whole genome shotgun sequence.
ACCESSION   DAOSZP010000032 DAOSZP010000000
VERSION     DAOSZP010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6908
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9320_length_6908_cov_3.510142"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            17..268
                     /locus_tag="PK348_01395"
     CDS             17..268
                     /locus_tag="PK348_01395"
                     /inference="COORDINATES: protein motif:HMM:NF023251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HXK64897.1"
                     /translation="MNYYLLLLPSVHDVMKAEKILLNAGVNVNVIATPREISHNCGVV
                     IRFESSNLHKLLALLEELPVEKKIFKKADDNVYVEVTQE"
     gene            851..1738
                     /locus_tag="PK348_01400"
     CDS             851..1738
                     /locus_tag="PK348_01400"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HXK64898.1"
                     /translation="MINRDSIDIKDLPVFPQVAIKILQIQEDNIDISFKELESIILLD
                     PALTAKILKVANSALYARQREITNLQQALTLLGFKMVKSLVLLVSASNVYSKNVKSQQ
                     QTTAATVTKTSTAMIWRHSVVTAFISKYVATRIKNDEKKEDVFVAGLLHDIGRLIMMI
                     SFRDKYEQYIAYLNQMQYKDIREIEEKIFEINHQDIGKIVLEKWNFPNELIDAVAQHH
                     VIQVDSKYKTTVQIVGLANLFAKIIENENLSENDRELLTAFSKALGITQEDYNYLTEE
                     IVNTLKNDELYKMSTSLIG"
     gene            complement(1746..>2160)
                     /gene="pelG"
                     /locus_tag="PK348_01405"
     CDS             complement(1746..>2160)
                     /gene="pelG"
                     /locus_tag="PK348_01405"
                     /inference="COORDINATES: protein motif:HMM:NF028243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="exopolysaccharide Pel transporter PelG"
                     /protein_id="HXK64899.1"
                     /translation="KQKMLLSLKNGIKGIIFFQSIWTVGILIKLPEILEFLGYLHTIN
                     IHIMRILLIAVFFHMLTLNLMIYLLYMELQFEAAMAACLYLILNIAATLFSILYFPLL
                     PGTSYMLASVATTLYCGYHLYKKAPIIDFIIFSRT"
     assembly_gap    2161..2170
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2171..3103)
                     /gene="pelG"
                     /locus_tag="PK348_01410"
     CDS             complement(<2171..3103)
                     /gene="pelG"
                     /locus_tag="PK348_01410"
                     /inference="COORDINATES: protein motif:HMM:NF028243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide Pel transporter PelG"
                     /protein_id="HXK64900.1"
                     /translation="MAGIGFELYKILHKGTLSSIVQAFFLGMIIVAGPWILSVLTIYI
                     IQTYTFGAIANNPALFTVSIVYVYAFSLFLSGGFHYVFSRYIADQLYIENYETIPTAL
                     LTAIIIITILSILPALAFVCFNDFSFIQFQTLYILSLVSLFVVINILWIMLVYIALLK
                     AYYKIFFSYLAGTIASIYGVLYLGKLYGVAGALCGYTIGQAVIVLLLIAISQITYPLK
                     KFTLNKEFIASFKEHKRIFLMGCFFNMAIWSDKMLYWFLQGQHIPGTLYYYYITYDIP
                     VFLAYLTMIPGLVYFLIIAETDFHKKFFAFISQIL"
     gene            complement(3110..4537)
                     /gene="pelF"
                     /locus_tag="PK348_01415"
     CDS             complement(3110..4537)
                     /gene="pelF"
                     /locus_tag="PK348_01415"
                     /inference="COORDINATES: protein motif:HMM:NF038011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GT4 family glycosyltransferase PelF"
                     /protein_id="HXK64901.1"
                     /translation="MDFVAINKPDPSKKTICIIAEGSYPYITGGVSAWIQDIITGLTN
                     YNFIIFAISAEEDEPKYLFPPNVIGLVNRLLTAPVEKPKKSIQSSFFSNIESFHNDMM
                     QKKDFSRFSEIFKLINKHRYDPSSLQRHITSFNIVTQYNEIRNPLYPFSDYYWACRSS
                     HEYMMKIMTWDLPPADLYHTISTGYAGLCAASAKIKYNKPVILTEHGLYNKEREIDIK
                     RATWVKGYQRDMWINIFNDLSAITYEYSDCVISLFEVNRNIQIAQGAKREKTLVIPNG
                     IEIDRYLDLKTETRKEFSVGIVARVVPIKDVKNFIIMAKIVAESIPDVKFYIIGPTDE
                     DESYYEDCVKLVENFQIQDKVVFTGKADVRQYYKFLDVVVLSSIREAQPLVILEAYAA
                     GIPVVSTRVGNVPEMLDYNDILLADPKDSEKLAQGVIYLYNNPDYRNKLIAANRNKVI
                     QFYNKKDLIATYDNLYAKYIGMYTQ"
     gene            complement(4525..5364)
                     /locus_tag="PK348_01420"
     CDS             complement(4525..5364)
                     /locus_tag="PK348_01420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK64902.1"
                     /translation="MSTILPHEDFIFDYTVSKSSKNIIKLYSFENPFDFFRHDVVENP
                     YIVKTQHKLRKAFSEGLLGIEHYLMAATSLRPLIDEAQDLDEITRLFSQQKLAMRPNP
                     HSVKILRTMIKDPNPEIALYAAEGLNTIENSFLTKIEKIKKKLEKATHNIFIYHYLLG
                     LLYTQFAALLESQKLIQKFYIKQAIGNLVKAYALRPKNKRIKIALADSYLLDENFEYA
                     LSLYAYCYSIQYKPVYCLLKMAQCHYNLKHYDRIKTIASIISQYDVSDIEFAEVLIYQ
                     WIL"
     gene            complement(5373..5969)
                     /locus_tag="PK348_01425"
     CDS             complement(5373..5969)
                     /locus_tag="PK348_01425"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HXK64903.1"
                     /translation="MGALNIEKIPFIKYNLYTEQLLLMIADLAAPIIRNAIRYSSLVH
                     KEVDPVTGFNSISEFYEVLKEEFANAQHNKLNLSLLIVECTNSAEIVEKFSMKDALLV
                     LKEIAQLVQQLAKGKVLLFQYKETFQFAAILPNMDFDGAAMFSLSLIEQNSKKAYYIK
                     GQIVNPEIVIGYSSLRPNHQSEEDLILLAENLLLMQKI"
     gene            complement(5933..6814)
                     /locus_tag="PK348_01430"
     CDS             complement(5933..6814)
                     /locus_tag="PK348_01430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HXK64904.1"
                     /translation="MDIIVKIFKKIFPLEIILLVTAVFIFNILKYPDDIGFLSIHYNP
                     YLFVIIFFTSFYGKKSGLITFLTVTIIIASYIIISDLYYSTDILYTTITTPVSYQHLS
                     SLLFLSLIAIIILGEIRDNLGRIIQNQKNIIKELDEQAAKLKRELEAVSMVNQEYQDR
                     ILGQQNSLISLYSTIIALNTLHLENIYPNILNAVVQFTGSQQCSLWQYDRDNNSIHLL
                     AYNGWTQDIIDSTPSNLDTENLIGWSVRNNTMFSIKMLQKYQNLKALDNGNSIITVPI
                     TIDNQVWGRSTLKKSLL"
BASE COUNT         2307 a         1093 c         1248 g         2250 t
ORIGIN      
        1 aacaaaggta taaaaaatga actattacct gttattattg ccatctgtac atgatgtgat
       61 gaaggctgaa aagattttac ttaatgcagg tgttaatgtg aatgtaatag ccaccccacg
      121 agaaatatcg cataactgtg gggtggtaat acgctttgaa tccagtaatt tacataagct
      181 attagccctt cttgaggaac tgcctgtaga aaaaaagatc tttaaaaaag ctgatgataa
      241 tgtgtacgtt gaagttactc aagagtagat tctaactttt taatgctctc atcaagtatt
      301 ttgtattcct tggaattttt tggaaatgta ctctggtact ttttaaagta ctttatctgt
      361 tccaatttcc agcgcttcat gtcaaaatta ttctggatca tattaattcc tccgtttaat
      421 aaactgtcgt actataattt tcgtaaaaaa agtaaaataa tttcattatt ttattgtttg
      481 aactattgtt atttgcaaat ttaatgatta tttaatatgt aacaatatat tagaatataa
      541 tacttgttat cgccagtaag ttagtatata ttaaaatcac ggaatttgtt acgtgaagga
      601 aatttttatg gaaggtgccg ttagtcaata gtacctgcat tagcttgtag atagaaagag
      661 gtaagaaaaa aagtatgata ttttatgttc tcttaagata acaggtgatg ataaggtaat
      721 tgtaacaaag acgtgatgga atgataaata aaattttgtt gataatggtt gtttgtaatg
      781 acgatagtat aatgataagc tataataaaa ttatttaaga ttattttaaa ttggtactta
      841 gtaggaagct atgattaaca gggatagtat tgatataaaa gatttaccag tttttccgca
      901 ggttgcaatt aagattttac agatacagga agataatatt gatatcagtt tcaaggagtt
      961 agaaagcatc attttgcttg atccggcatt gacagccaag atactgaaag tagctaattc
     1021 agcactctat gcgcgtcagc gtgagattac caatttacag caggccttga cgttacttgg
     1081 tttcaaaatg gtaaaaagcc ttgtgttact ggtttcagct tcaaatgttt acagtaaaaa
     1141 tgtaaaatcg cagcaacaga caactgccgc aactgttacc aaaacatcaa cagcgatgat
     1201 ctggcggcac tcggttgtta ccgcattcat ttcaaaatat gtggcaacac gcattaaaaa
     1261 tgatgaaaaa aaagaggatg tatttgttgc tggtttgctt catgatatcg gcaggctcat
     1321 catgatgata agtttcagag acaaatatga gcaatacatt gcatatctca accagatgca
     1381 gtataaggac atacgcgaaa ttgaagaaaa aatatttgag ataaaccatc aggatattgg
     1441 caagatagtg ctggaaaaat ggaattttcc caatgaactg atagatgctg tggcacagca
     1501 tcatgtaata caggttgact caaaatataa gactacagtt cagatagtag ggctggcaaa
     1561 tctgtttgca aaaattattg aaaatgaaaa tctttcagaa aatgacaggg aattgcttac
     1621 tgccttcagc aaggcattag gtatcaccca ggaagattat aactatctca cagaagagat
     1681 tgtcaataca ctgaaaaatg atgaattata taaaatgagc acttcactga ttggataaac
     1741 ttattttatg tcctgctaaa aataataaaa tcaattatag gtgctttttt atacagatga
     1801 tagccacagt atagtgttgt tgctaccgat gccagcatat agctggtacc tggaagtaac
     1861 gggaagtata gtattgaaaa taacgttgct gcaatgttta aaataagata cagacatgca
     1921 gccattgcag cttcaaattg caattccata tacaaaagat aaatcataag attaagcgtc
     1981 aacatatgaa agaatacagc tataagcaga attctcataa tgtgaatatt aattgtatgc
     2041 aaatacccca aaaattccag tatctctggt agctttatta aaataccaac ggtccatatt
     2101 gactggaaaa atataatacc cttaatacca tttttaagtg ataacagcat cttttgtttt
     2161 nnnnnnnnnn cagtatttgt gaaataaatg caaagaattt tttatgaaaa tctgtttcag
     2221 caataattaa aaaatatacc agtccgggta tcatagtcag gtatgccaaa aacaccggta
     2281 tatcataagt gatgtaatag taatacagtg taccaggaat atgctgtccc tgtaaaaacc
     2341 agtaaagcat tttatccgac catatggcca tattaaagaa gcatcccatt aaaaaaattc
     2401 gtttatgttc tttaaacgat gcaataaatt ctttatttag tgtaaatttt ttaagggggt
     2461 atgtaatctg ggaaatagct attaaaagca aaacaatcac agcctgacca attgtgtacc
     2521 cgcacaatgc acctgcaacg ccatacaatt ttcctaaata aaggacgcca taaatactgg
     2581 cgatagttcc tgctaaatag ctgaagaaaa ttttgtaata tgctttgagc agggcaatat
     2641 ataccagcat gatccacagg atgttaatca caacaaaaag tgaaactaac gatagtatat
     2701 aaagagtttg aaattgaatg aatgagaaat cattaaaaca cacaaatgca agagcaggca
     2761 gtattgacag tatggttata ataattattg ctgttaacag ggctgtgggg atggtttcat
     2821 aattttcaat atacagttga tcagcaatat agcgtgagaa tacataatga aaaccacctg
     2881 ataaaaatag tgaaaaagca tacacataca ctatcgaaac agtaaaaagt gcaggattgt
     2941 tagcaattgc cccaaatgta tatgtttgaa taatatatat agtaagtact gacaatatcc
     3001 atggtcctgc aacaatgatc atgcccagaa agaaagcctg tacaattgat gacaacgttc
     3061 ctttgtgtaa aattttatat aattcaaatc ctatcccggc catacatcct cattgtgtat
     3121 acatgccaat atattttgca taaagattgt catatgttgc aataaggtct tttttattat
     3181 aaaactgtat aaccttattc ctgtttgcag ctatcagttt attacggtaa tcaggattat
     3241 tgtatagata aataacaccc tgggctaatt tttctgaatc tttggggtcg gctaaaagaa
     3301 tatcattgta atcaagcatt tcaggaacat tgcccacgcg ggttgagacc acaggtattc
     3361 ctgctgcata ggcttctaag ataaccagtg gttgcgcttc ccggatactg gaaagtacca
     3421 ccacatctaa aaatttgtaa tactggcgca catcagcttt accggtgaat actactttat
     3481 cctgtatctg gaaattttca acaagcttaa cgcaatcttc ataataactt tcgtcttcat
     3541 cagttgggcc aataatgtaa aatttaacat cagggattga ttctgcaaca atctttgcca
     3601 tgatgataaa attcttaaca tcttttatgg gaacaactcg cgccacaatc ccaacactga
     3661 attccttgcg ggtttctgtt tttagatcta aatatcgatc tatttcaatg ccattgggta
     3721 taacaagtgt tttttcgcgc tttgccccct gtgcaatctg aatattgcga ttaacctcaa
     3781 aaagagatat aacacaatca gagtattcat acgtaatagc gcttaaatca ttaaatatgt
     3841 taatccacat atctcgctgg tagcctttaa cccacgttgc gcgttttatg tctatctcac
     3901 gttctttgtt gtataaacca tgctcagtca ggattacagg tttattgtat ttaatctttg
     3961 cactggcagc acatagccct gcatatcctg ttgatatggt atggtacagg tcagccggtg
     4021 gcaggtccca ggtcataatc ttcatcatgt attcatgtga tgatcgacat gcccagtaat
     4081 agtcagaaaa gggatataag ggatttcgta tttcattata ctgagttaca atgttaaaac
     4141 ttgtaatatg tcgctgcaga gaagatggat cgtagcggtg cttattaata agcttaaaaa
     4201 tttcactgaa tcgagaaaaa tctttttttt gcatcatatc attgtgaaag gattcaatat
     4261 tgctgaaaaa cgatgactgt attgactttt tgggtttttc aactggggct gtcagtagcc
     4321 tgttaaccag tccaatcacg ttgggtggaa ataaatattt tggttcgtcc tcttctgctg
     4381 atattgcaaa tatgataaag ttatagttgg taagccctgt aattatatcc tgtatccagg
     4441 ctgatacccc tcctgttatg tatggatatg acccttctgc gataatacat attgtttttt
     4501 ttgatggatc aggtttattg attgctacaa aatccattga tagattaaca cctcggcaaa
     4561 ttctatatcg cttacatcgt actgagaaat aattgaagca attgttttaa ttctgtcata
     4621 atgttttaaa ttataatggc attgagccat tttgagcagg caatatacag gtttgtattg
     4681 tatagaataa caatacgcat agagtgatag tgcatattca aaattttcat ccagtaagta
     4741 gctgtctgcc agagctattt ttatcctttt gttttttggt cgtaatgcat atgcttttac
     4801 caggtttccg attgcctgtt taatataaaa tttctggatt agcttctggc tttctaaaag
     4861 tgcagcaaat tgtgtataaa gaaggccaag caaataatgg taaataaaga tattatgggt
     4921 agctttttcc agctttttct ttattttctc aatttttgtt aaaaatgaat tttcaattgt
     4981 gtttaatcct tcagcagcat acagggctat ttctggattg ggatctttaa tcatggttcg
     5041 taatattttt actgagtggg ggttagggcg catggctaat ttctgctggg aaaataaccg
     5101 tgtaatttcg tccaggtcct gggcttcatc aattaatggt cgtaatgaag tagctgccat
     5161 aaggtaatgt tctataccca gcaagccttc agaaaaagcc ttacgcaatt tatgctgagt
     5221 tttaacaata taaggatttt caacaacatc atgcctgaaa aaatcaaaag gattttcaaa
     5281 ggaatacagc ttaattatat ttttgctgct cttagataca gtatagtcaa aaataaaatc
     5341 ttcatgagga agaatggtgc tcatgctctc acctatattt tctgcattaa cagtagattt
     5401 tcggccagta gtataaggtc ttcttcagat tgatggttgg gtcgcaagct gctgtatcca
     5461 atgactattt cagggttaac tatctggcct tttatataat atgctttttt actgttttgt
     5521 tcgattagcg ataaactaaa cattgctgca ccatcaaaat ccatgttggg taaaatagct
     5581 gcaaactgga atgtttcctt atattgaaat aataatactt ttccctttgc cagttgttga
     5641 actaactgag ctatctcttt taatacaagg agtgcatcct tcattgaaaa cttttcaact
     5701 atttcagcgc tattagtaca ttcaactatc agtaacgaca aattcaattt attatgctga
     5761 gcgttagcaa attcttcctt taatacctca taaaattcac tgattgaatt aaaaccggtt
     5821 acggggtcaa cctctttatg taccagtgag ctatacctga tagcattacg gataattgga
     5881 gctgccaggt cagcaatcat gagcagtaac tgttcagtat agagattata ttttataaaa
     5941 gggatttttt caatgttgag cgcccccata cctggttatc aatagtaatg ggaacagtaa
     6001 taatggaatt gccattatca agtgccttca agttttgata tttctggagc attttaatgg
     6061 aaaacatggt attgtttcgt accgaccagc caataaggtt ttccgtatca aggtttgaag
     6121 gggtactatc aatgatgtcc tgtgtccacc cattatatgc aagcagatga atagagttat
     6181 tatcacggtc atattgccac aatgagcatt gctgagaacc ggtaaattga acaacggcat
     6241 tcaagatatt aggataaata ttttctaaat gtaatgtatt taaggcaata atggttgaat
     6301 acaatgagat aagtgaattt tgctggccta aaatacggtc ctgatattcc tgattaacca
     6361 ttgaaacggc ttcaagttct cttttaagct tggcagcctg ctcatcaagt tctttgataa
     6421 tatttttctg gttttgaata atgcggccta aattgtcacg tatttcgcca agaataataa
     6481 tagcaataag cgatagaaat aataatgacg atagatgctg gtaactaacc ggtgtggtga
     6541 tagttgtata cagtatgtct gttgaataat acagatcact gattataata tatgatgcta
     6601 tgattatcgt tactgtaagg aatgtaataa gacctgattt tttgccataa aacgaagtaa
     6661 agaagattat aacaaataga tatggattgt agtgtataga aagaaaacca atatcgtcag
     6721 gatatttgag aatattgaag atgaatactg ctgttacaag aagaatgatt tcaagtggaa
     6781 atattttttt aaatattttt actatgatat ccataagaaa cagtatattt gtgttaagtt
     6841 ataataaagt gtattgttca atactaataa caaaatttag taaatgtcaa ggaaattaga
     6901 aagtataa
//
LOCUS       DAOSZP010000033         6861 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_9409_length_6861_cov_4.882016, whole genome shotgun sequence.
ACCESSION   DAOSZP010000033 DAOSZP010000000
VERSION     DAOSZP010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6861
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9409_length_6861_cov_4.882016"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            298..1404
                     /locus_tag="PK348_01435"
     CDS             298..1404
                     /locus_tag="PK348_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002610807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HXK64905.1"
                     /translation="MKKGLLFIIVLLVTIALTPYGVFASVARTTYPVVFAHGMAGFDN
                     ILGYDYWGDDYGVFVGDPCDEFLEVTCNANINSGQKSFIAQMQPFQSSEYRGTQLANQ
                     IESYMATTGTRYINLVGHSQGGMDIRKAAKVLYQHKGYQVVKVMVSISSPHRGSPLGK
                     FVLQKGTLGKIVEFLFNVYGNVVYKSGNDAVAALKQFTYDDYDPNDGVITGAKVFNQN
                     YPVSSTYAARYVSLITAQQSADLNPALLFVRALYDIDGNGYCVDDGDNDGAGGCGNGI
                     KNDWDDDGMVGINSQQMGYRLKYTEVFLGQDYCTTDTNLGYVGNINYPNAAQMTSTAD
                     LINQDHLDVIGLPPDTFDEMEFYAAIFDYIANYD"
     gene            1645..2682
                     /locus_tag="PK348_01440"
     CDS             1645..2682
                     /locus_tag="PK348_01440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK64906.1"
                     /translation="MKKQYWYGMIVLFIFVGIAFIIFHKSDTVQSRIDYIKNGGVVPP
                     VNMLLKAFEADEELQARLHADFDMQDVIQYFRDKYGKTINHKYTQVMLIEKLRSYLMQ
                     MYPDDWVVYLYAMVREIFPGMENEILDTFEKLNKYDTWLKDNNGDLAKMDTKDRRDML
                     WKKRYEIFGQDAEDVWAAEKQTEPIYSALDTIKNSNDMTFNEKLTLYKDSIKEAYKNG
                     APDSVARKQQELMNTFLDVEGIQKDLAAMAPEERKENLKELRKAMGLDNAALQRWDEL
                     DQVRDQRWDAGMQYMTERTELEKHYTGSELDKRLDSLRMKYFGDEAATIKAEEDSGYY
                     RFKQQRKYGME"
     gene            complement(2708..6229)
                     /locus_tag="PK348_01445"
     CDS             complement(2708..6229)
                     /locus_tag="PK348_01445"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HXK64907.1"
                     /translation="MELKYGVTVWGNYFLEAIYQYYADIKRLQRGLRYANSDRVKKIE
                     INNTLVLAKVKGNQGRSYTVSLYFSPFKSDEKEKVVTLIEDNLLVLGEIIKGNLPDIL
                     LDELTKNNIKLFPSDWKELNRGCSCPDWGDPCKHMAAVYYLLTREIDLNPFLLFTLRG
                     LDLTKLFKITTKSLIDYPINIQEAHENQYNNELQSFREVAIVTINNYTNFILSKLSNN
                     PSFIDKDFKPIIEKFYKKSQALSDAILLRDIASMDSDLLLKLENLFKSGKISFIPDKN
                     FVGSYFTFYSELIEHEPVRQKIDSLNIMVYDAKTSTYHISPLNMLKLFLQFTTMYESN
                     ESTSECLMYNFLFILSRVAYNFVKSNAFIPAVYLEKNQFYCIFRLLSSPVDIKKQIHN
                     VISAMPVSLTLPSLTGKNTKKSRGTMDKESTIKFMLACFFTEYASIIGTSMKSSLKLT
                     KQFHSTMYDAIFLSKPYSMERFNDKALPLAIANYFSIFELIESNLDFSVIIDKHNDEY
                     FLQFLVNNTTINQITEQKTKNIVLKVLSNFIDVLPEIPLLLKNSTLPVSQERLEDLIT
                     KKLELLQNLGVEVLLPKELKEIIKPQLKIKTRLKDKTHVSFFSLNDMVTFDYEFYIDN
                     ETISYEELIALMNQNKELLKFRDSYIRINTTDIAKLMQQYETKSNLSQFDLLKSHLSN
                     ESETIELCDEIKAMFETMFTEQQYQLPHLNASLREYQERGFNWMLHLLLHGFGVILAD
                     DMGLGKTLQAIAVIQFLKENGYIKKPMLIVVPKTLMFNWESEIKKFSENLSYTFYYGT
                     KRTFSDTDIIFTTYETLQRDYKKLEDIVYDGIILDEAQKIKNYQTLTAKAIKHLSKKA
                     KFRIALSGTPIENRLTELWSIFDFILPGYLKSIDYFVNEYTKEIELKRNQQKIENLRK
                     ITSPFILRRLKTDKSIIKDLPEKIIIDQFVEMEKEQIALYEHVVQQLREEIAASENLL
                     PIILKSFTILKQICNHPRNYDKKSPAISHISGKTKLLLEILPAIIAKQEKVLIFTQFV
                     EMGNILKQIIENELLSEPLFLTGNLSQIKRKELIEKFEHDDSAQIFILSLKAGGVGLN
                     LTKANHVIHYDLWYNPSVENQATDRAFRIGQKKNVFVHRFVTKSSFEEKINAMIQHKR
                     ELNDLVITEGETWLTRLNKDELLDLLG"
     gene            6381..>6861
                     /locus_tag="PK348_01450"
     CDS             6381..>6861
                     /locus_tag="PK348_01450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK64908.1"
                     /translation="MQHGKLYSIMLCVLLVTGCSDKVTVTECGIFAPSRLHYVKGQDG
                     VTPVDFDAQHTMWTFGDTITHEGMIANSLAFTNKVDASNIRELPFHYYTENGEIVPFI
                     RNDKHEDPSRDRLWAFDGIRINNTVYVYYVHVYIHDPLKPLSFSLMESSVAHWDVPYN
                     "
BASE COUNT         2137 a         1076 c         1333 g         2315 t
ORIGIN      
        1 aagtgctcaa atgaaatgca tgttgttgtt tctatgctca tgaagccctc ccaaaaccgt
       61 gatgcatgtt atcataaaaa agtaataacg aatattgtgc aatcataact acacaataaa
      121 atactcaata ccaatgctgt ggcatggtaa atttcagcat ttttacaata aaattaaaaa
      181 aattctaacc agtcggtatt tatttcttga ctttttctgc ttctatatta gtatatacca
      241 tatagacggt atttattatt atatttgttg tttacagaga gttaaggagg cgtcattatg
      301 aagaaaggtt tgctattcat tattgtttta ctggtaacta tcgctctgac cccgtatggt
      361 gtgtttgcgt cagtagcacg aacaacctat ccggtagtat ttgcacacgg tatggctggt
      421 tttgacaata ttctgggata tgactattgg ggcgatgatt acggagtttt tgttggtgat
      481 ccctgtgatg agtttttaga ggttacctgt aatgctaaca ttaattcagg tcagaaatca
      541 tttattgcac agatgcagcc atttcaaagt tctgaatatc gtggtacgca gttagccaat
      601 cagattgaaa gctatatggc aacaaccggt actcgttata taaatttggt aggacactca
      661 caggggggca tggatatccg taaggctgct aaggtattgt atcagcataa gggatatcag
      721 gtagtaaagg tgatggttag catttcatca ccacatcggg gttcaccatt agggaagttt
      781 gttcttcaaa aggggacatt gggcaaaatt gttgaatttt tatttaatgt ctatggaaat
      841 gttgtttata agtcaggtaa tgatgctgtt gcagctttga aacaatttac ttatgatgat
      901 tatgacccca atgatggtgt aatcaccggt gccaaggtat tcaatcaaaa ttatcctgtt
      961 tcatcaacgt atgcagcacg ctacgtttcg ttgataacag cacagcagag tgcggatctt
     1021 aacccggcat tgctctttgt cagagctctc tatgacatag atggcaatgg ttactgtgtt
     1081 gatgacggtg ataatgatgg cgcaggagga tgtggcaatg gtattaaaaa tgattgggat
     1141 gatgatggta tggttggcat taactcgcag cagatgggct acaggctaaa atacaccgag
     1201 gtatttttag gacaggatta ttgtacaacc gatactaatt taggatatgt tggcaatatc
     1261 aattatccta atgccgctca gatgacttca actgccgatt tgataaatca ggatcacctg
     1321 gatgttattg gtttgccgcc tgatacattt gatgaaatgg agttttatgc agcaatattt
     1381 gattatatag caaactacga ttaattgtat agtacagcgc actttcataa aaatccttct
     1441 ccatacagtg gaggaggatt tttttagatg agggcaggta aaaagtaagt gtggcataat
     1501 gctttggcga tagttactgt tgaatggtat gttgctgtca aatactattt gtagcatgga
     1561 agtataaaaa cctggttaaa tagtatacat gacatgattc gcactaaaac aatgatacca
     1621 tgattacatt atggggataa aagtatgaaa aaacaatatt ggtatgggat gatagtatta
     1681 ttcatatttg tgggaatagc ttttattata ttccataaaa gcgatacggt gcaatcgcgt
     1741 attgattata taaagaatgg tggggtggta ccaccggtga atatgctgct aaaagcattt
     1801 gaagctgatg aagaattaca agcacggctc catgctgatt ttgatatgca ggatgttata
     1861 cagtatttca gagataaata tgggaaaaca atcaaccata agtatactca ggtaatgtta
     1921 attgaaaagt taagaagtta tcttatgcag atgtatccgg atgattgggt tgtatatctg
     1981 tatgctatgg tgcgtgagat atttcctggt atggaaaacg agatacttga tacatttgaa
     2041 aagttgaata agtatgatac gtggctaaag gacaacaatg gtgatctggc aaagatggat
     2101 acaaaggata gacgcgacat gttgtggaaa aagcgatatg aaatttttgg gcaggatgcc
     2161 gaagatgtat gggcagcaga aaaacaaacc gagcccatct acagcgcact tgacactatc
     2221 aagaatagca acgacatgac atttaacgaa aagttgactt tatataaaga tagtattaaa
     2281 gaagcatata aaaatggtgc tcccgatagt gttgcccgca aacagcagga gcttatgaat
     2341 acctttcttg atgttgaagg tatacaaaaa gatcttgcag ctatggctcc cgaagagcgt
     2401 aaggaaaatc taaaagagct acgcaaggca atggggcttg ataatgcagc attgcagcgc
     2461 tgggatgaat tggatcaggt tagagatcag cgctgggatg ccggtatgca gtatatgacc
     2521 gagcgtacag agctggaaaa gcactataca gggtcagagc ttgacaaacg gcttgatagt
     2581 ttgcgcatga aatattttgg tgatgaagca gctacaataa aagctgaaga ggacagcggg
     2641 tattataggt ttaaacagca aagaaaatat ggaatggaat agtaaaaata ggcttttata
     2701 aacaatgtta tccaagcaaa tcaagcagtt catctttatt gagccgtgta agccatgttt
     2761 cgccttcggt tatcactaaa tcgttcagct ctcttttatg ttgaatcatg gcattgattt
     2821 tttcttcaaa tgaactttta gtaacaaacc tgtgtacaaa gacatttttt ttctgcccaa
     2881 ttctaaaggc tctatcggta gcctggtttt caacagatgg gttataccaa agatcataat
     2941 ggataacatg atttgctttt gttaaattta aaccaacccc tcctgctttt aatgaaagta
     3001 taaagatttg tgcgctgtca tcatgttcaa atttttcaat gagttctttt cttttaatct
     3061 gagataaatt gccggttaaa aataatggtt ccgataataa ttcattttca ataatttgtt
     3121 ttaatatatt tcccatttct acaaattggg tgaagattaa taccttttct tgtttcgcta
     3181 taattgctgg taaaatctca agcaataatt ttgttttccc tgagatatgt gaaatagcag
     3241 gacttttttt atcataattt cggggatgat tgcatatttg ctttaatatt gtaaagcttt
     3301 tcaatataat gggaagcaga ttttcgcttg cggcaatctc ttctctgagt tgctgaacaa
     3361 catgttcata gagtgcaatt tgctcttttt ccatttcaac aaattgatca atgattatct
     3421 tttcgggtaa atctttgatg attgatttgt cagttttaag cctgcgcaaa ataaacggtg
     3481 atgtaatctt tcgtaaattt tcaatttttt gctgattacg ctttaattct atttcctttg
     3541 tgtattcgtt aacaaaataa tctattgatt ttaaataccc tggcaatata aaatcaaaga
     3601 tactccagag ctctgtaagc ctattttcta taggtgtgcc actcaatgca atacgaaact
     3661 ttgctttctt tgatagatgc ttaatagctt ttgcagttaa tgtttgataa ttttttatct
     3721 tctgtgcctc atcaaggatt atgccatcat atacaatatc ctccagtttt ttataatccc
     3781 tctgtaatgt ttcgtaggtg gtaaatatga tatcagtatc actgaatgtt cttttagttc
     3841 cataataaaa tgtatacgaa agattttcag aaaatttttt aatttctgat tcccagttaa
     3901 acattaatgt tttgggaaca acaataagca ttggtttttt tatgtaccca ttttctttta
     3961 aaaattgtat aacagctatt gcctgtaatg tttttcccaa acccatgtcg tcggctaaaa
     4021 ttactccaaa tccatgtaaa agcagatgga gcatccagtt aaagcctcgt tcttgatatt
     4081 ctctcagcga tgcgttgaga tgaggaagct ggtactgctg ctctgtgaac atggtttcaa
     4141 acattgcttt gatttcatca cacagttcaa tcgtttctga ttcattgctg agatgagatt
     4201 ttaaaagatc aaattgcgac aggtttgatt tggtttcata ctgttgcatg agcttggcaa
     4261 tatcagttgt gttaatccgt atatagctat ctctaaattt aagtagctcc ttattttggt
     4321 tcattaaagc aataagttcc tcataggaaa ttgtttcatt gtctatataa aactcgtaat
     4381 caaatgtaac catatcgtta aggctaaaaa acgatacatg tgttttgtct tttaagcgcg
     4441 tttttatttt aagttgaggt tttataatct cttttaattc ttttggtagt aaaacctcta
     4501 ccccaaggtt ttgtaacagt tcaagttttt ttgttatgag atcttccaat cgctcctgtg
     4561 atacaggcag agtgctattt tttaatagta acggtatttc aggtaaaaca tcaatgaagt
     4621 ttgataaaac tttcagtact atattttttg tcttttgttc ggtaatttga ttgatggttg
     4681 tattgttgac taaaaattgc aaaaagtatt catcattatg tttatctatg ataacagaaa
     4741 aatcaagatt tgattctata agttcaaaga tagaaaaata attagcaata gccaatggta
     4801 aagctttatc attgaagcgt tccatactat atggttttga taaaaatata gcatcgtaca
     4861 tggtactgtg aaattgcttt gttaacttta acgaagattt cattgatgtt ccaattattg
     4921 aagcgtattc agtaaagaaa catgccagca taaatttgat agtggattct ttatccatgg
     4981 taccacggct tttctttgta tttttaccgg tcaatgatgg caatgttagt gagacaggca
     5041 tggctgatat tacattatgt atctgctttt taatatcaac aggcgatgaa agcaatcgaa
     5101 agatacaata gaactgattt ttttctagat aaacagcagg gataaaggca tttgatttta
     5161 caaaattgta tgcaacacgc gaaaggataa acaaaaaatt atacatcaaa cattcgctag
     5221 tagattcgtt tgattcatac atggttgtga attgaagaaa taatttgagc atgtttaatg
     5281 gtgatatatg gtatgtgctg gttttggcat catataccat tatgttgagg ctatctattt
     5341 tttgacgcac cggttcatgt tcaatgagct cactatagaa ggtaaaatat gaaccaacaa
     5401 aatttttatc cggtataaaa gagatcttcc cggatttaaa aagattttcg agcttcaaca
     5461 ataaatcact gtccatcgat gctatatccc tcaaaagaat agcatcgctt aaggcttgtg
     5521 attttttata aaatttttca ataatgggtt taaaatcttt atcaataaat gatggattat
     5581 ttgatagttt tgataatata aaattagtgt aattattaat agtaactatc gccacttcgc
     5641 gaaagctttg caattcgttg ttgtattggt tttcgtgtgc ttcttgaata ttgatgggat
     5701 aatcaatgag cgatttagtg gtgattttga acagttttgt taaatccaat ccacgtaatg
     5761 taaataataa aaatggattt aaatctattt ctctggttaa tagatagtat actgctgcca
     5821 tgtgcttgca cgggtcgccc cagtcagggc agctgcatcc tctattcaat tccttccagt
     5881 cagaagggaa taatttaata ttattttttg ttagttcatc aagcagtata tcgggtagat
     5941 tgcctttgat gatctcgccc aatacaagta gattgtcttc tatgagcgta acaacctttt
     6001 ctttctcgtc gcttttgaaa gggctaaaat agagcgacac tgtatatgat ctgccttgat
     6061 ttccttttac ttttgctaaa acaagggtat tgttaatctc aatcttcttt actctgtcgc
     6121 tatttgcata tcgcaatccc cgctgtaacc gttttatgtc agcatagtac tggtagatag
     6181 cctctaaaaa ataattaccc cataccgtta cgccatattt caattccata gccatgctct
     6241 ataaaaaaag attataataa aaattactaa tagctctgat aaagccgttt aatcaacaaa
     6301 aaaagaaaaa ataattgaaa aaaagtagta tttatcgata gtcatacgat agtaaaaaag
     6361 gtaaaggcta atcatacagt atgcaacatg gtaaacttta tagcattatg ttatgtgttt
     6421 tactcgttac tggttgttca gataaggtta cagtaactga atgcggtatc tttgctcctt
     6481 cacgtcttca ctatgttaaa gggcaggatg gtgtaacgcc agtagatttt gacgcacagc
     6541 ataccatgtg gacattcggt gacaccatta ctcatgaggg gatgatagct aatagtctgg
     6601 catttaccaa taaagttgat gcaagcaata tcagggaatt accattccac tattacactg
     6661 aaaatgggga aattgttcca tttatccgga atgacaaaca tgaggaccct tcaagagaca
     6721 gattgtgggc ttttgatggt atacggatta ataacacggt gtatgtatac tatgtccatg
     6781 tctatatcca tgatccgttg aaacctctgt cattttcatt gatggaaagc tcggttgctc
     6841 actgggatgt tccctataac t
//
LOCUS       DAOSZP010000034         6817 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_9496_length_6817_cov_3.601154, whole genome shotgun sequence.
ACCESSION   DAOSZP010000034 DAOSZP010000000
VERSION     DAOSZP010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6817
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9496_length_6817_cov_3.601154"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1031)
                     /gene="serC"
                     /locus_tag="PK348_01455"
     CDS             complement(<1..1031)
                     /gene="serC"
                     /locus_tag="PK348_01455"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007938641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HXK64909.1"
                     /translation="MARIYNFASGPSILPLPALEEASRELVDYRHTGMSLIEMSHRGK
                     IYDEVHNEAIALIKEILQVPDDYYILFIQGGATLQFGMIPIAFLGPGKIADFILTGTW
                     ARKAHEDAKLIGNPHVIWDGASEKYRRIPDQRELQYTPGAEYVHICSNETIDGIQWQK
                     MPTTGNIPLIVDMSSDIMSRPVEWDKVSMLFAGTQKNLAPAGMALVVMKKELVEKARK
                     DVPAYLRYDLHAKENSLYNTPPTFTVWMTTLTLRWIKSIGGLPEIEKRRDEKAAILYN
                     AIDSSNGYYNCPVDKHSRSKMNVVWRLKNEQLEEKFVKEAEKEGLSGLKGHRSVGGLR
                     ASIYNAMPV"
     gene            1170..1859
                     /gene="rsmI"
                     /locus_tag="PK348_01460"
     CDS             1170..1859
                     /gene="rsmI"
                     /locus_tag="PK348_01460"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013252935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HXK64910.1"
                     /translation="MSGKLYIIATPIGNMEDITLRALRILKEETDIVYCEDTRQTAKL
                     LNNYDIHVPIRSLHTHSSKARLQSIIQELESGKTIAYLTDSGTPGISDPGGQLVALAR
                     KHNICVIPVPGASAVTAIISVCGFPATQFYFAGFLSKKEGKRKNQLQKLSHLDAIIVL
                     YESPYRIIKLLHAIAEVMPQAQILIGREMTKHFEEFIYCTARDIEDIAATLTQKGEFV
                     VAVYNAKKTYM"
     gene            1947..3137
                     /locus_tag="PK348_01465"
     CDS             1947..3137
                     /locus_tag="PK348_01465"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXK64911.1"
                     /translation="MKYYPRTLEKTLAHYLKLFPAVAVCGPRQSGKSTMLKKQYGHKY
                     RYITFDDPIVRERFNIDPKGFLHEYNNQVIFDEVQKVPGIFEYLKMLIDANRSQYGNF
                     ILTGSSQFSMVTHITESLAGRIGVLTLLPFQFSEIPQSLQKHQIIYGSYPENVVRKYQ
                     GVHEWYAAYITNYIERDVRSLFNIGNLKDFQRLLSLLASQTAQELNMNRLANDIGVTV
                     KTIKSWISVLEASYILFTISPYYNNIGKRIVKRPKIYFYDTGLVCYLTGMHTVDILHK
                     GPLAGPMFENYIIAELKKLLVHYNKRMNLYYLRDNASNEIDVIIEDPLNRKLILTEIK
                     HTATPKPLMAQRLQKLVTLVKNHIPAYTVHGMILYQGKEDFYVHDVRFANYKHFLDEL
                     ERHF"
     gene            3333..3626
                     /gene="flgM"
                     /locus_tag="PK348_01470"
     CDS             3333..3626
                     /gene="flgM"
                     /locus_tag="PK348_01470"
                     /inference="COORDINATES: protein motif:HMM:TIGR03824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HXK64912.1"
                     /translation="MVIDKIGNIKNIVEPKSTKSVGKTRSTGKSDSVEISSEAKKAAE
                     QSKIAQVVKQTSDIRAERVREIKEQIANGTYDFNDDRIIDKVAEKIAMQLLRK"
     gene            3654..4880
                     /locus_tag="PK348_01475"
     CDS             3654..4880
                     /locus_tag="PK348_01475"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HXK64913.1"
                     /translation="MAGIIDKKAESGFRFFMSKAYGQTIEGLWPDLYIPTAVYAQPDS
                     VTLLPSIIKLFGSRILFITSSIDFNQYNHLFMPIARSLQNNNIPFMVYDEIPENNTDY
                     IDTAVYFARKTHCDLVIGFGGINSINAAKAVALLTPNYFFAHELFTYPFLHEPIPFIS
                     IPVHPLFGFDITPCTILTDIHDGIHKTFFHNYIFPKAVILDPALAFGIDDETMARYSI
                     ATLAIATESVISRNINNITNTLSLRTIDLIFKNLPAALRESDNETARLNVMMASLMAG
                     IAFSSAQLSVCLALSLALSSLTPIDLASYMAVMLPHIMEYNLTSAPGKYVQMSKVMDE
                     DVRDITVIEAAIKAVEGVRKLALDVEIPQRLSQFDINKGIFSKVAEITLSYPFIHNAP
                     RALNRDEVETILIAAY"
     gene            4884..5633
                     /gene="larB"
                     /locus_tag="PK348_01480"
     CDS             4884..5633
                     /gene="larB"
                     /locus_tag="PK348_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HXK64914.1"
                     /translation="MSQSALNNLLQRFKNGLVSEEDFLQELSHITIEQLGHTTLDHHR
                     HTRLGFPEVIFCQNKTPEQVADIAKALAKRNTSFLGTRATKEHYNKVRRHIKKAHYNE
                     AGKIISYRAQTLHHKPHKLVIITAGTSDIPVAEEAKETALIMGLTVETIYDIGVAGIH
                     RVFHNWEFIHDATVIIVIAGMEGALPSVIGGLVPCPVIAVPTSVGYGASFGGIAALLG
                     MLNSCAPGVSVVNIDNGFGAVYCAYRIIRSL"
     gene            5643..6806
                     /gene="larC"
                     /locus_tag="PK348_01485"
     CDS             5643..6806
                     /gene="larC"
                     /locus_tag="PK348_01485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HXK64915.1"
                     /translation="MKILYCDIQLGASGDMLVGALIDMGLPVDVITTQLQSLSVKGFA
                     IAPAKVERYGIHGTQAGITAHTDTTHRNLDSIYNIIAHTPCSDTVKTNAMKIFDTLAT
                     AEATVHGISKEDVHFHEVGAIDSIVDILSFCIGIEYFSIDTLYYSTFALGTGTINTQH
                     GMLPLPAPATVKLTHGKQVMFTGLQGELTTPTAAAILVTLGTQQTQFGGTIVTTGTGF
                     GTRDYGFPSYTRTLLIEDNSNEYEVLSVLECTIDDMNPEIYPHVAELLFEAGARDVYL
                     QQVIMKKGRPGVVISVLCPFHAVDQCTQILFRQTTTLGVRAYTVNRYSLPREFATVIL
                     YGEEIRIKQSYRYQELSPKPEFEDCKKVALKTGKPLYHIMEEALKEYYKQKKL"
BASE COUNT         2158 a         1357 c         1276 g         2026 t
ORIGIN      
        1 tcaacaggca tggcattgta gatagaagca cgcaaacctc caacagatcg gtgccccttt
       61 aagccactta agccttcctt ttctgcttct ttcacaaatt tttcttcaag ttgttcattt
      121 ttcaaacgcc ataccacatt catctttgag cggctatgtt tatcaacagg acaattataa
      181 tatccattgc ttgaatcaat agcattgtac agtattgcgg ctttttcgtc gcggcgtttt
      241 tcaatttccg gcaatcctcc aattgattta atccacctga gtgtaagtgt tgtcatccac
      301 acagtaaatg ttggcggtgt attatacagc gaattttctt ttgcatgcag gtcatagcgt
      361 aaatatgctg gtacatcttt tcttgctttt tctacaagtt cttttttcat aacaaccagt
      421 gccatgcctg ctggagccag atttttctga gtgcccgcaa aaagcatgct caccttatcc
      481 cactccactg gccttgacat gatatccgat gacatatcaa caattaatgg aatattaccg
      541 gtagtgggca tcttttgcca ctgaatgcca tcaatagttt cattggaaca tatatgtaca
      601 tactcagccc ctggtgtata ctgtaactct ctctggtctg gaatgcgtcg gtatttctca
      661 gaagccccat cccagataac atgaggattg ccaatgagct ttgcatcttc atgcgctttt
      721 cttgcccagg tacccgttaa tataaaatca gcaattttac caggcccaag gaatgctata
      781 ggtatcatac caaattggag agtagcacca ccctgtatga acagaatata gtaatcatcc
      841 ggtacctgca aaatctcttt aataagagct atcgcctcat tatgaacttc atcgtatatt
      901 ttgccacggt gactcatttc aatgagagac ataccagtat gcctataatc aacaagttcc
      961 ctgcttgctt cttccagtgc tggcaatggt aatattgaag ggcctgatgc aaaattatag
     1021 atacgcgcca tgtattctat tctccttatg gaagtataac tattctaaaa gcggttatta
     1081 cgtattaata ccacattcta cttgctaaaa aagctattga tatgatactg ttataaaaaa
     1141 tcaagcaaat aaatccagag ggatacccca tgtcaggaaa actgtatatt attgcaacac
     1201 caattggtaa tatggaagat attacattac gggctttacg tatccttaaa gaagaaactg
     1261 atatagtcta ttgcgaagat acacggcaaa cagcaaagct tcttaataat tacgatatac
     1321 atgtacccat acgttcactg catacccatt caagcaaagc cagactacag agtattattc
     1381 aggaattaga gtcaggcaaa acaatagcct acctcactga ttcaggtacc ccaggaatat
     1441 cagatccagg aggtcaactg gtagcactgg cacgaaagca caatatttgt gtaatcccag
     1501 taccaggggc ttcggcagta acagctatta tttcagtgtg cggttttcct gcaacacaat
     1561 tctattttgc aggtttttta agcaaaaagg aaggtaagcg aaaaaaccag ttacaaaaac
     1621 ttagtcacct tgatgctatt attgttttgt atgaatctcc ctaccgcatc ataaaactat
     1681 tgcatgcaat tgccgaagtt atgccacagg cacagattct cattggcagg gaaatgacca
     1741 aacattttga agaattcatt tattgtacag cacgtgacat tgaagacatt gctgcaaccc
     1801 taacacaaaa aggggaattt gttgtggcag tgtataatgc taaaaaaacg tatatgtaaa
     1861 agtggaagtg gatgattaat attaaattat atattatttt acatttttat attgacagta
     1921 taaatttgta aatatatagt aaactcatga aatattatcc aagaacatta gaaaaaacgt
     1981 tagctcatta tttaaaactt ttcccggctg ttgcggtatg cggtccacgg caatccggaa
     2041 aatccaccat gttaaagaaa caatatggtc ataagtaccg ctatatcaca tttgatgatc
     2101 caattgttag ggaacgtttt aacattgatc ccaaaggttt tttacacgaa tataataacc
     2161 aggtcatctt tgatgaagtt caaaaagtgc cgggaatttt tgaatatctt aaaatgttaa
     2221 ttgatgcaaa tcgttctcaa tatggtaatt ttatacttac cggatccagc caattttcaa
     2281 tggttacaca tataaccgaa tctttagcgg gtaggattgg agtcttaacc ctattgcctt
     2341 tccaattttc agagattccg caatcacttc aaaagcatca gattatatac ggcagttatc
     2401 ctgaaaatgt agtgcgaaaa taccagggtg tacatgaatg gtatgctgca tacattacaa
     2461 attacattga acgcgatgtc cgttcacttt ttaatattgg aaacttaaaa gattttcaac
     2521 ggttactatc gctactggcc tctcaaactg ctcaggaact aaatatgaac aggttagcca
     2581 atgacattgg tgtaaccgta aaaacaatta aatcatggat ttcagtactc gaggcaagtt
     2641 acatactatt tacaatatca ccatattaca acaacatagg aaaacgaatt gtaaaaagac
     2701 caaagattta tttttacgat acaggccttg tatgctacct gaccggcatg catactgtag
     2761 atatattaca caaagggccc ttagcgggac caatgtttga aaattatatt attgctgaat
     2821 taaaaaaatt actggtacac tataataaaa gaatgaattt atattacctc agagataatg
     2881 cgagcaatga aattgatgta ataattgaag atccattaaa caggaaattg attttaaccg
     2941 aaataaaaca cactgcaaca cctaaaccac tgatggcaca acggttgcaa aaattggtaa
     3001 cgctggtaaa aaatcatatt ccagcatata ctgtacatgg catgatactg taccagggaa
     3061 aagaagattt ttacgtgcat gatgtgcgtt ttgctaatta taagcatttt cttgatgaac
     3121 tagaaaggca tttttagtgt atatttgatt ctctttttgt tttttgttct ataataaata
     3181 tgattaacat catcacgtta atcattattc taccctgata ttaatttaat tacttgatta
     3241 attcctctat taatgtatat ttattacaaa atcatttaag atttgacaaa taatagccga
     3301 tagttactat agattaaaag agggtgatta ccatggttat tgataaaata ggaaacatta
     3361 agaatattgt tgaacctaaa agcaccaaat cggttggtaa aacaagatcc actggtaaaa
     3421 gtgactcggt tgagatttca agtgaagcta aaaaggcagc agagcaatcc aaaattgccc
     3481 aggttgttaa acaaacatct gatataaggg cagaaagagt acgcgaaatc aaggaacaga
     3541 ttgccaatgg cacatatgat ttcaatgatg accgtataat agataaagtt gcagaaaaaa
     3601 ttgccatgca gcttttacga aaataactac acccttatta aaggttatta tagatggccg
     3661 gcataattga caaaaaggcg gaatcaggat tccgcttttt tatgtctaaa gcctatggac
     3721 aaactataga aggcctgtgg ccggatctat acattccaac tgcggtgtat gcacaacccg
     3781 atagtgtaac acttcttcct tcaataatta aactttttgg atcacgtata ttatttatta
     3841 ccagttcaat tgattttaac cagtataacc acctttttat gcccattgca cggagcctgc
     3901 aaaacaataa cataccgttc atggtatatg atgaaattcc tgaaaacaat actgattata
     3961 ttgatactgc ggtgtatttt gcccgcaaaa cccattgcga cctggtcata ggatttggcg
     4021 gtattaattc aatcaacgct gcgaaagcag ttgcactgtt aacccctaac tatttctttg
     4081 cacatgaact tttcacctat ccttttctac atgagccaat tccatttatc agcattcctg
     4141 tacatccact gtttggattt gatataactc cctgtacaat attgactgat attcatgacg
     4201 gtatccacaa aacatttttt cacaattaca tcttccctaa agcagttatc cttgaccccg
     4261 ctcttgcatt tggtattgat gatgaaacta tggcccgata tagtatagca acattagcta
     4321 ttgcaactga atcagtcatt tcaagaaata tcaataatat taccaataca ctttcgctga
     4381 gaactataga cctcatattt aaaaatttac ctgctgcatt gcgcgaaagt gacaatgaga
     4441 ctgcacggct taatgtgatg atggcttcac taatggctgg tattgcattt agtagtgcac
     4501 agctctctgt ctgtcttgct ctgtcactgg cactttcttc attgacacca attgatttag
     4561 catcatatat ggctgttatg ctaccacata ttatggaata taatttaaca tcggcgcccg
     4621 ggaaatacgt ccagatgtca aaagttatgg atgaggatgt acgtgatatc accgttattg
     4681 aagctgcaat taaagctgtg gaaggtgtaa gaaaattagc tcttgatgtt gaaattcccc
     4741 aacggttatc ccaatttgat ataaataagg gcattttttc caaagtagct gaaattacct
     4801 tgagctaccc atttatacat aatgcaccgc gtgcactcaa ccgcgacgaa gttgaaacca
     4861 ttctcattgc tgcctattag cttatgagtc agtctgcact taacaattta ctgcaacggt
     4921 ttaaaaatgg tctggtatcc gaagaggatt ttttacagga actatcgcac ataacgatag
     4981 aacagttagg ccacaccaca ctggatcacc acagacatac caggttaggt ttccccgaag
     5041 ttattttttg ccagaacaaa acacctgagc aggttgccga tattgctaaa gcgttagcca
     5101 aacgtaacac aagcttttta ggtacacgtg ccacaaaaga gcattacaac aaagtaagac
     5161 gacacattaa aaaagcccat tataatgaag caggcaagat catttcttac cgtgcacaaa
     5221 cacttcacca taaaccacat aaactggtaa ttataacagc ggggacatcg gacataccgg
     5281 ttgctgaaga agcaaaagaa accgcactca ttatggggct tactgttgaa actatctatg
     5341 atattggcgt tgctggtatt caccgtgtat ttcataactg ggaattcatt catgacgcaa
     5401 cagttatcat tgtgatagct ggtatggagg gagcgctgcc aagcgttatt ggagggcttg
     5461 tcccctgccc ggttattgct gtaccaacct cagtgggata cggtgcttca tttggtggta
     5521 tagcggcgct tttggggatg ctcaactcat gtgccccggg ggttagcgtt gttaacattg
     5581 acaacgggtt tggcgctgtg tactgtgcct atcgtattat acggagtttg taggatagtt
     5641 acatgaaaat actttactgc gatatacagt taggcgcttc cggtgatatg cttgttggtg
     5701 cacttattga tatgggactt cctgttgatg ttattaccac acagctacaa tcattatcag
     5761 taaaaggttt tgcgatagcc cctgcaaagg tagagcgtta tggaatacac ggcacacagg
     5821 ctggaattac cgcacacact gacaccacac accgtaatct tgatagcata tataatatta
     5881 ttgcacatac gccatgctct gacactgtta aaaccaatgc aatgaagata tttgacacat
     5941 tagccaccgc tgaagctaca gtacacggca tttcaaaaga agatgttcat tttcatgagg
     6001 ttggagccat tgacagcatt gtggatatac tttctttttg tattggtatt gaatactttt
     6061 caattgatac actgtattac agcacatttg cgcttggaac aggcactatc aacacacagc
     6121 atggcatgtt gccgttacct gcacccgcaa cggtaaagct tacccacggc aaacaggtta
     6181 tgttcactgg actgcagggt gaactgacca caccaacagc tgctgcaatc ctggtaacac
     6241 ttggcacaca acagacacaa tttggcggta ctatagtaac tacaggtacc ggttttggta
     6301 cacgtgatta tggttttccc agttatacgc gaactttgct tattgaggat aattctaatg
     6361 aatacgaagt tctgagtgta ctggaatgca ctattgatga catgaatccc gaaatatatc
     6421 ctcatgtggc cgagttgtta tttgaagccg gtgcgcggga tgtatatttg cagcaggtta
     6481 ttatgaagaa agggcgcccc ggggtggtga tttcagtact ctgccctttt catgcggtgg
     6541 atcaatgtac acagattctt ttcaggcaga caacaacgct gggggtcaga gcatataccg
     6601 tcaaccgcta ttcgttgcca cgagagtttg caacagtaat cctgtacgga gaggaaatcc
     6661 gtattaagca aagttaccgt tatcaggaac tatcgccaaa acctgaattt gaggattgca
     6721 aaaaagtagc attaaaaacg ggaaagccct tatatcatat catggaagag gctctgaaag
     6781 aatactataa acagaaaaaa ttatagctgc ttcaatt
//
LOCUS       DAOSZP010000035         6766 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_9596_length_6766_cov_3.545224, whole genome shotgun sequence.
ACCESSION   DAOSZP010000035 DAOSZP010000000
VERSION     DAOSZP010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6766
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9596_length_6766_cov_3.545224"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..682)
                     /locus_tag="PK348_01490"
     CDS             complement(<1..682)
                     /locus_tag="PK348_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK64916.1"
                     /translation="MINKIMVFIIVVYITSSSAYAFLENYNNYQYLIGDRAAGFGGAY
                     CAIANDSTALWYNPAGLANINDNRLNISANTYSYLTRNSRQYWQIEESPGQYQCLDLK
                     ETDISVVPNTVAYARRVNWLGNDVIAYGLFVPIQDSIQATIAGKATGSVLNVELNASY
                     RINTKSYYGVAGYGVNITKNLKLGLSAGVGYFQGKGGANISAYLDPGGTNQSEIAMII
                     DNELTALTA"
     gene            1051..1299
                     /locus_tag="PK348_01495"
     CDS             1051..1299
                     /locus_tag="PK348_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012899471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HgcAB-associated protein"
                     /protein_id="HXK64917.1"
                     /translation="MQNNNCCNSSCSIEAVVSVDERGQLVLPKDLREKYGIKAGDKFV
                     VLTLNSTESLCCIAFVKADSLNSVVFEQIAPVFSNLGK"
     gene            1302..2090
                     /gene="arsM"
                     /locus_tag="PK348_01500"
     CDS             1302..2090
                     /gene="arsM"
                     /locus_tag="PK348_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013330730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HXK64918.1"
                     /translation="MDTNTKIKKIVQKNYGTIALSKTSCCSAGCCNPTDVASKIGYSA
                     NEMNELDSANLGLGCGNPNAIASLKQGEIVLDLGSGAGIDCFLAAKKVGQHGRVIGVD
                     MTPEMIALAKENAKKLRVNNVEFILGDIEHLPVDDNSVDVIISNCVINLAPDKERVFK
                     EAYRVLKRGGRMAISDIVLDGTLPPYIKESIDAYVSCIAGAILKSEYIALLHKAGFSN
                     VEIVKEYGYGNIFASNECNNDGTSYDGQEQYKEIVKSISLVAWK"
     gene            2646..4019
                     /locus_tag="PK348_01505"
     CDS             2646..4019
                     /locus_tag="PK348_01505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXK64919.1"
                     /translation="MKEGYVVPTIEKIAYGLGDLAINIAYTTIGFYFLFFLVNVAGLP
                     AQWAGIIFFIARAWDAITDPLMGMISDRTKSKYGRRRVYFLFGCIPFGLMFMLIWLVP
                     SHDHYILLGYYAAITILFNTAFTVVAVPYNALMPELSQNYDERTSISGFRMSLSFVGN
                     LIAAVGVSVIVDVVFKGKEHYITSYPVMGVIFGIIIIVLIFITFVGTKERVQSEAVIH
                     DGLLKTFKAVLSLHEFRIILGMFLFNMIGFDLIQALFIFFLKDVILIEEDMTFVVMGI
                     PLIVAVLSAPLWIYISDKLDKRRAYMVAAIYFTLSLLLCLIAPVHNLQFVIIIGVLAG
                     VGISASQIIPFSMIPDVIEYDEYKNGVRREGAFYGITTFLYKVASASAIALASAFLAF
                     FGYIENSATAQPQSAVSAIRYMMAFAPGLCFLISVYFVSILPITREGFDEIKRILNER
                     KSIANNG"
     gene            4128..4658
                     /locus_tag="PK348_01510"
     CDS             4128..4658
                     /locus_tag="PK348_01510"
                     /inference="COORDINATES: protein motif:HMM:NF039195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PsbP-related protein"
                     /protein_id="HXK64920.1"
                     /translation="MRRKYLLCISIIIFSVMILPGCSRKEKGRFYSDEYNFSIVFPKH
                     WEIQQQEMGAVVVALSPEEGMRDDFRENVAITVEEVSGDSTLDKYFELSQKNLKSYSK
                     DYRLLYKNNTWLARKPAMILGFTYTMSGLKLKVLQYYCLNNNKVYVITCTALPHTFDR
                     YEETFLKIVRSFRFEK"
     gene            complement(4683..5504)
                     /locus_tag="PK348_01515"
     CDS             complement(4683..5504)
                     /locus_tag="PK348_01515"
                     /inference="COORDINATES: protein motif:HMM:NF020476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1848 domain-containing protein"
                     /protein_id="HXK64921.1"
                     /translation="MIISASRRTDIPAFYSQWFIDCIQKGHCSVTNPFNPNQRKEVSL
                     LPSDVDAIVFWTRYPVPLMKYLGQLTDSGYRYIFLYTITGYPSWLEPHSPELSHAIQA
                     FKDVSRILGSQKVVWRYDPLVFSKELDFDFHIKNFAAIAQQLQGYTQRVIISIMEPYK
                     KVTRRLKVLHEKGIVTYPMNIFGQDELFKFFTCITHIAFDCGMQIHSCCSNLTEFGIE
                     NGACIDGNLINSMFGLDLHFEKDIYQRTNCLCAKSVDIGTYNTCKFGCLYCYASR"
     gene            complement(5525..6259)
                     /locus_tag="PK348_01520"
     CDS             complement(5525..6259)
                     /locus_tag="PK348_01520"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HXK64922.1"
                     /translation="MKIYKNISIIYEDNHIIVVEKPAGMLSQKDYTDTDSLLEILKEY
                     IKTTYKKPGNVFLGLVHRLDKQVSGIMVYAKTSKAASRLSSQFRSHDVIKIYCAALQT
                     ITSTDTAWHTIHDAIIRHHDKTLISSDNITAMHASLKYKIIYTKPSYSLALIQLYTGK
                     KHQIRAQMSHLGMPIANDIKYGATLISSEIAIALHAVYLQCIHPVTKERISFYSKPQG
                     IIASIFNTIDVDHVIKNALIHEGYSL"
     gene            6478..>6766
                     /locus_tag="PK348_01525"
     CDS             6478..>6766
                     /locus_tag="PK348_01525"
                     /inference="COORDINATES: protein motif:HMM:NF017026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HXK64923.1"
                     /translation="MQKHEHKGKSSSVYINAHELIAKLPITNGMHILDAGCGDGYFSI
                     ALSEKFPDSTIYAMDYNEQAIDNLNIVVKEKRLTNMQPMPGDITKDIPVKAD"
BASE COUNT         2134 a         1066 c         1330 g         2236 t
ORIGIN      
        1 atgcagtaag tgctgtaagt tcattatcaa taatcattgc aatttcactt tgatttgttc
       61 cacctggatc aagataggct gaaatgtttg caccaccttt cccctgaaag taaccaacac
      121 ctgccgataa accaagtttc aggtttttag tgatattaac tccatatcct gcgaccccat
      181 aatatgattt tgtattgatt ctatatgaag cattaagttc aacatttaat acactacctg
      241 ttgctttgcc agcaatagtt gcctgtattg aatcctggat gggaacaaat aatccataag
      301 caataacatc atttcccagc cagtttactc tgcgggcata ggcaacggtg tttggcacaa
      361 ctgaaatatc ggtttctttt aaatcaaggc actgatattg tccaggtgat tcttctattt
      421 gccagtattg tctggaattt cgggtaagat aactataggt attggctgat atgttcaatc
      481 tattgtcatt aatatttgca agacctgcag gattatacca cagcgcagtg ctatcattgg
      541 caattgcaca atatgctcca ccaaaaccag ctgctctatc accaatgaga tattgatagt
      601 tgttataatt ttcaaggaaa gcatacgcac tgctactcgt aatataaaca actataataa
      661 ataccataat tttgttaatc attgggaacc tccataattt cataagtgta tatttttctt
      721 gctaaacaaa ttaataataa tgtagtaaaa aaacaaatga ttattttttc ttttaataca
      781 aagtattagg aaactacttt tgtttaaaat atattaggtt tatgatattg ataattttga
      841 aataaagtga acagtgccag agcatgagat tgtcaaatta tctttctgct aaaagtaaaa
      901 ggggtcagag cacatataac atttctgcaa aaaaaagaat ttgtgaaaat tttgttgaca
      961 aaacagcaat aaaataatca atatcagaaa tatcagaaaa tacagaatat tctgataaaa
     1021 cattatatca tgattaataa ggataaaact atgcaaaata ataactgttg caattcttcg
     1081 tgttcaatcg aagcagtggt ttctgttgat gagcgcggac aattagtatt gccaaaagat
     1141 ctcagggaaa agtatgggat aaaagcagga gacaagtttg tcgttttaac tcttaatagc
     1201 actgagtcgt tatgttgtat tgcttttgta aaggccgatt cattaaattc agttgttttt
     1261 gagcagatag ctccagtatt cagcaattta gggaaatgag tatggatact aatacaaaaa
     1321 ttaaaaaaat cgtgcaaaaa aattatggca caatagccct ttcaaaaact tcatgttgtt
     1381 ctgctggttg ttgcaatccc acagatgttg cttccaaaat tggctattca gctaatgaaa
     1441 tgaatgaact tgattcagca aatttagggt tgggatgtgg caatcccaat gcaatagctt
     1501 ctttgaaaca gggggagata gttcttgatt taggcagcgg tgcaggcata gactgttttc
     1561 ttgcagcaaa aaaggtaggt caacatggca gggttattgg tgtagatatg acgcctgaaa
     1621 tgatagcact tgcaaaagaa aatgcaaaaa aactaagagt taataatgtt gaattcatac
     1681 tgggcgatat tgaacattta cctgttgatg acaattcagt tgatgtgata atttcaaatt
     1741 gtgttataaa tttagcacct gataaagaac gggtattcaa agaagcttac cgggtattga
     1801 aacgtggtgg tagaatggct atatccgaca ttgtgcttga tggaacctta ccgccatata
     1861 ttaaagaatc tattgatgcg tatgtaagtt gtatagctgg ggcaattcta aaaagtgagt
     1921 atattgcgtt gttacataaa gcgggttttt caaatgttga aattgtaaaa gaatatggtt
     1981 atggcaatat ttttgcaagt aatgaatgta acaatgatgg cacatcatat gatggtcaag
     2041 aacagtataa agagatagta aaaagtatta gtctggttgc ctggaaataa ataatttatt
     2101 ttccttgatt tttgcttgcc actatagttt tgtattaaca atattcacat atcggtcact
     2161 tcgatatacg tgctttgcat gcacttggtg atcaattttc ataataaggt cgtcgagtgt
     2221 ttggagctaa gcaaggcata tatcgagaca acgagaattt tcattctgac cccgatataa
     2281 ttgggggaag aatctcagtt ataaagtaat aaaacggcaa gatccttccc gattacatcg
     2341 ggattgcatc aggacttaaa gtttggactc ctcaggatga cagtgagggt attacgttgt
     2401 gacttgcaat aacgttgatt gcacgttaca gcgagcaatg tgtgatagtc tcaggtatat
     2461 aaatgggatt accatgttct aacttcatcg ggaaccgcaa atacggtgtg tgaaacggcg
     2521 agattcttca tcacgatttc attgggactc agaatctaat aatttatgta aatttctatg
     2581 tttgatatag catgtaattc tttattattc agatatatat caataatata aaaaggtatt
     2641 tgccaatgaa agaaggttat gtagtcccca ctattgaaaa gattgcatat gggttaggtg
     2701 atttagcaat taatattgcc tataccacaa taggttttta ctttttattt tttttagtca
     2761 atgttgcagg cttacctgcg cagtgggctg gcattatctt ttttattgct cgtgcatggg
     2821 atgccataac ggatccgctc atgggtatga tttctgacag gactaaatct aaatatggaa
     2881 ggcgaagggt ttattttctg tttggttgca ttccttttgg tcttatgttc atgttaatat
     2941 ggctggttcc ctctcatgat cattatatac tgttgggtta ttacgcagca atcaccatac
     3001 tgtttaatac tgcttttact gtagtggcag ttccctacaa tgctctgatg ccggagctat
     3061 cgcaaaacta cgatgagcgg acaagcatat caggctttag aatgtcactt tcatttgttg
     3121 ggaatctgat agcagcagtt ggtgtttcgg taattgtaga tgtagtcttt aaaggcaaag
     3181 aacactatat tacaagctat cctgttatgg gtgttatctt tggtatcata attatagtat
     3241 tgattttcat aacatttgtg ggaactaaag aacgagttca gtctgaagcg gtcatacatg
     3301 atggtctttt gaaaacgttt aaagctgttc taagtttgca tgaattcagg attattttag
     3361 gaatgttttt atttaatatg attgggtttg atttgattca ggcgctattc atctttttcc
     3421 tcaaagatgt tattctgatt gaagaagaca tgacttttgt tgttatgggt atacctctca
     3481 ttgtagcggt actatcagcc ccattgtgga tttatatttc agacaaactt gacaaacgca
     3541 gagcatatat ggttgctgct atatatttta cgctatcgct tttgttatgt ttgatagctc
     3601 cagtacataa tttgcagttt gtaataatca ttggtgtact tgcaggggtt ggcatatcag
     3661 catcacagat aatcccattt tccatgatac ctgatgttat tgaatatgat gaatataaaa
     3721 acggtgtgcg acgtgaaggt gctttctatg gcattacaac gtttttatat aaggttgctt
     3781 cagcatcagc tattgcactg gcatcagctt ttctggcttt ttttggatat attgaaaaca
     3841 gtgccacagc acagccacaa tctgcagttt cagctatacg atatatgatg gcatttgcgc
     3901 caggtttgtg ctttttaatt tcggtgtatt ttgtttcaat actacccatt acacgggaag
     3961 ggtttgatga aataaaaaga atattaaatg aaagaaaaag cattgctaat aatggttaac
     4021 aatattttat gatataatta aagaatgtgt aatataccat tataataaat gaagtataca
     4081 ctatatagta ttatacagat atgttctatt caatctggag caaacgtatg agacgaaaat
     4141 acttactatg tatatcaatc attatattca gtgtaatgat actacccggt tgttcacgga
     4201 aggagaaggg caggttttac agtgatgaat ataatttttc cattgtattc ccaaaacact
     4261 gggagataca gcagcaggaa atgggtgctg ttgttgttgc attgagtcct gaagagggaa
     4321 tgcgtgatga tttcagagaa aatgttgcca taacagttga agaagtttca ggtgatagta
     4381 ctcttgataa atattttgag ctttcacaaa aaaatttaaa aagctattca aaagattaca
     4441 ggctactgta taaaaacaac acgtggctgg cacgcaaacc tgcaatgatt ttaggattta
     4501 catatactat gagtgggtta aaattaaaag tgttgcagta ttattgcctc aataacaata
     4561 aagtgtatgt tattacatgt acagcactac ctcatacatt tgaccgttat gaagaaacgt
     4621 ttttaaagat agtgaggtca tttcggtttg aaaagtagta tgggcgatag ttactgtttt
     4681 tattacctgc tggcgtaaca gtataaacaa ccaaacttac atgtattgta tgtcccaatg
     4741 tcaacacttt ttgcacacag gcaattggta cgctggtata tatctttttc aaaatgtaaa
     4801 tctaacccaa acatagaatt gataaggttg ccgtcaatac atgcgccatt ttctattcca
     4861 aactcagtga gattactgca gcaactatga atctgcatgc cacaatcaaa cgctatgtgc
     4921 gttatgcagg taaaaaactt gaaaagttca tcttgcccaa aaatattcat tgggtatgtt
     4981 acaattcctt tttcatgcaa tacttttagc ctgcgtgtaa cctttttgta tggttccata
     5041 atgctgataa taactctttg tgtgtagccc tgtaattgtt gtgctattgc agcaaaattt
     5101 tttatgtgaa aatcaaaatc caattcttta gaaaatacta gcggatcata gcgccatact
     5161 actttttgtg atcccagtat cctgcttaca tctttaaatg cctgtattgc atgcgatagt
     5221 tctggtgaat gaggctcaag ccagctggga tagcctgtaa tggtgtataa aaagatgtaa
     5281 cggtatcctg aatcagtcaa ctggcctaaa tatttcatga gtggtacagg atatcgcgtc
     5341 cagaagacta ttgcatcgac atctgatggc agcaacgata cttcctttct ctgattggga
     5401 ttaaatggat tggtgacaga gcaatgacct ttttgtatgc aatcaataaa ccactgtgaa
     5461 tagaatgcag ggatgtcagt tcttcggctt gcactgatta tcatggttac tatcgcccta
     5521 aatgttatag tgaatagcct tcatggatca atgcattttt aattacatgg tcaacgtcaa
     5581 tggtattaaa aattgaagca ataatgcctt gaggtttgga ataaaaggat atacgttctt
     5641 ttgtaacagg atgaatacat tgcaggtaca ccgcatgcag ggcaattgca atttccgatg
     5701 atatcaaagt tgctccgtat ttaatgtcat tggcaatggg catgccaaga tgcgacatct
     5761 gtgcacgtat ctggtgcttt ttgcccgtat acagttgaat cagtgccagt gaataggatg
     5821 gtttggtata aataatttta tatttgagtg atgcatgcat ggcagtaatg ttatcactag
     5881 aaatcagtgt tttgtcatgg tgtcggatga ttgcatcatg gattgtatgc catgcagtat
     5941 cagtgctggt gatggtttgt agtgccgcac agtatatctt gatgacatca tggcttctga
     6001 attgagacga aagacgtgaa gcggctttcg atgtctttgc atacaccatg attccggaaa
     6061 cctgtttgtc aagcctgtga acaagaccaa ggaatacatt gccaggcttt ttataggtgg
     6121 ttttgatata ttctttaaga atttcaagca acgaatcagt atcagtatag tccttctgac
     6181 ttaacatgcc tgcaggtttt tctacaacaa tgatgtgatt atcttcgtaa ataatggaaa
     6241 tattcttata gattttcatg gtaaaggtga tatcttcaaa gtatactatt cataccgtat
     6301 atgtaccaag tattatagtg caatattgtt atgtccactg taaaatattt gtttattgat
     6361 acataatttt ccgttacaat tgaatcataa tagggcatat tacacagggg taattatgta
     6421 attgcctttg tggatattta taaaacattt taaaagcaaa tatcataagg agatccaatg
     6481 caaaaacacg aacacaaagg gaaatcaagc agtgtatata taaatgcaca tgaacttatt
     6541 gcaaagcttc ctataacaaa tggaatgcat attctggatg ctggttgtgg tgatggatat
     6601 ttttcaattg ctctttctga aaagtttcct gattcaacaa tttatgctat ggattacaat
     6661 gaacaggcaa tagataacct gaatatcgta gtaaaagaaa aacggcttac aaatatgcag
     6721 ccaatgcctg gtgatataac aaaagatatt cctgttaaag ctgacg
//
LOCUS       DAOSZP010000036         6734 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_9648_length_6734_cov_3.788741, whole genome shotgun sequence.
ACCESSION   DAOSZP010000036 DAOSZP010000000
VERSION     DAOSZP010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6734
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9648_length_6734_cov_3.788741"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2023)
                     /locus_tag="PK348_01530"
     CDS             complement(<1..2023)
                     /locus_tag="PK348_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015706698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HXK64924.1"
                     /translation="MITVGINIGSTSVKVVVCNSGVVETFNIMPHEGDVPGTLTTILS
                     QLNIPKDARAIVTGNEGRFLLHLPRVIESVCIEEALKHSDYRPDAIVSMGGEDLVVYT
                     VDEHKKIKNNFSGNKCAAGTGEFFKQQLARMNMKLADIHSIPHESKVLRLSTRCSVFM
                     KSDCTHRLNKGEATKGDIILSLSNVMAVKVVDFLKKARIHNGTVIITGGITRNPYIVQ
                     FIKEKLPDIDFIIPDTAAYFEAYGAALLAQQYGEPIGDTLFEKHTISFPRFNSLKTAL
                     NNVTYIPSKRGVAKPGGEYILGVDGGSTTTKACLVDIHTHEIVAAHYGRTHGDPVKAL
                     KQCIEEIQKQLKDQIGDNPITISLAATTGSSREILGVFLETSAIYNEIIAHSVGTTYF
                     KPDIDTIFEIGGQDAKYVYLKNGVPIDYAMNEACSAGTGSFLEESAQGDLNIAHAWEI
                     GDIALQAEQPLKFGEHCSAFINSDIRKAIQQGASREDITAGLITSIVANYLNRVVGNR
                     TIGNSIVLQGGVAKNKAVPLAFAMLLGKHITVPPDPELMGCFGVAIVALNKLHDGLLQ
                     KKFYSLDGILNTDIVYEREFTCKACDNYCPIRVLNVNGNRYMFGGRCNKYANMRKKRV
                     IHEEEVVDFIEKRNELLFHECAPQEMPHNASITVGVPLCFSAYTLWPLYS"
     gene            complement(2090..3002)
                     /locus_tag="PK348_01535"
                     /pseudo
     CDS             complement(2090..3002)
                     /locus_tag="PK348_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513420.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
     gene            complement(3107..3775)
                     /gene="hypB"
                     /locus_tag="PK348_01540"
     CDS             complement(3107..3775)
                     /gene="hypB"
                     /locus_tag="PK348_01540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HXK64925.1"
                     /translation="MEIQVLQNIYESNIQKAEEIKKLLSQKKVYMFDIMSSPGAGKTT
                     ITDRIINDLIKDYNIALIEGDIETTNDAQRLAKYNIPIVQIETKLFGGACHLESSWIL
                     GCLEKFDLNAVDVVIIENVGNLVCPAEFELGDDERVVVLSVTEGEDKPVKYPLMFNSS
                     HTLLINKIDLLPYLDFNMDELLANVKRTNPKMEVHKVSAKTGEGIEGFLKALRSRIKN
                     KVGR"
     gene            complement(3882..5030)
                     /locus_tag="PK348_01545"
     CDS             complement(3882..5030)
                     /locus_tag="PK348_01545"
                     /inference="COORDINATES: protein motif:HMM:NF024571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXK64926.1"
                     /translation="MIFIGARQTGKTTILRQIQKSLEKESTAVYFINLEDPDYLSLLN
                     ENPKNLLKLFSFDLNKKSYVFIDEIQYLTNPSNFLKYFYDEYKGKIKLCVSGSSAFYI
                     DKKFNDSLVGRKKLFYVRTLSFKEFLRFKNENKLIDNDFKNLSITEKEKISFYYHEYM
                     LYGGYPKVVLSDFDQKIDLLMDIAYSYVKKDIYEAHIKSNEVFYKLCKILASQIGGLV
                     NTSELANTLGISKTAIDNYIYILQKSFHISLVRPFYMNVRKELTKMPKCYFMDTGLRN
                     FFVSSFESFKTRKDKGQLLENACFRQLVDSNNEDNIKFWRTIQGNEVDFIVNEKIAYE
                     VKVDENQFRKSKYSVFMQMYKSIPIAIVTLDKKVEMVEGCQVFDVWEI"
     gene            complement(5242..>6734)
                     /locus_tag="PK348_01550"
     CDS             complement(5242..>6734)
                     /locus_tag="PK348_01550"
                     /inference="COORDINATES: protein motif:HMM:NF033181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HXK64927.1"
                     /translation="RVVDAKSKGDMFRGFEMILKGRNPVDANQITQRICGVCPVSHGI
                     ASSKCLDAAFGIVPNKNGRLLRNLVFGANYLQSHILHFYHLAALDYVDITAILKYNGS
                     DKKLLALRDWAKNELEVKKGRQDAITAVAPFLPRYEGDFYIKDLDLNIAAIAHYVQAL
                     DMRMKAHQMVALFGGRVPHLIGLVPGGVTQVPTRSAIRQYRKLLKQVEEFVNEVYIND
                     VIAVAKAYSDYFKIGRYTNFLSYGVFEENPEQTEFFFKRGVFDGKKLQEFDSSKIREQ
                     VRFARYNSGSNLHPYQGQTNPDPHKAGAYTWLKAPRYDGMPMEVGPLARVVVNYLGGN
                     ADVKKEVDALLKVFNADIDAVFSTLGRHAARAIECKLLCQRMYEWLDDLEVGEMPRST
                     YEIPDKGQGEGLTEAPRGALGHWIVVEDKKIANYQCVVPTTWFCGPRGDDGKLGPVEQ
                     ALLGTPITDPRNPIEAARVVRSFDPCIACAVHVVEGDKEIGTFKIC"
BASE COUNT         2053 a         1446 c         1071 g         2164 t
ORIGIN      
        1 atgaatacag cggccataag gtatatgcag aaaaacacag cggtacacca accgttatag
       61 aggcattgtg tggcatttcc tgtggtgcgc attcatggaa caatagctca tttctttttt
      121 caataaaatc aacaacctcc tcttcatgga taacgcgttt tttccgcata tttgcatatt
      181 tattgcatct gccgccaaac atgtacctgt tgccattgac attgagtacc cgtatggggc
      241 aatagttatc gcaagccttg catgtaaact cgcgctcata gacaatgtct gtattaagaa
      301 ttccatcaag cgaatagaat tttttctgta agaggccatc gtgcaactta ttcagggcta
      361 ctatcgccac accaaaacaa cccatgagtt ccgggtcagg cggcactgtg atgtgttttc
      421 caagaagcat ggcaaatgca agcggtactg ccttgttttt tgccacacct ccctgcaaca
      481 caatggaatt gccaatagtc ctgtttccca caacacggtt gagatagtta gccactattg
      541 atgtaataag acctgcagta atgtcctccc tgcttgctcc ctgctgaata gccttgcgta
      601 tatcagaatt aataaaggct gaacaatgtt ctccaaattt tagtggttgt tctgcctgta
      661 atgctatatc gcctatttcc catgcatggg caatattcag atcgccctga gctgattctt
      721 ccaaaaacga accggtaccc gccgagcagg cttcattcat ggcataatca atgggtacac
      781 catttttcaa atatacatac tttgcgtcct gaccacctat ctcaaatatt gtgtcaatgt
      841 ctggcttaaa gtaggtagta ccaacagaat gggcaatgat ttcattgtag attgccgacg
      901 tttcaagaaa gactcccagt atttcacgtg atgaaccggt agttgctgca agcgaaatgg
      961 tgatgggatt atcacctatc tgatctttta actgcttttg aatttcttct atacactgct
     1021 tcaatgcctt aacggggtcg ccatgggttc gtccatagtg tgctgctact atctcatgag
     1081 tgtgtatatc aacaaggcat gcttttgttg tggtggatcc accatcaaca cccaatatat
     1141 actctccacc aggttttgca acaccacgct ttgagggaat atatgtaaca ttattaagtg
     1201 ccgtttttaa actgttaaac cgaggaaagc ttatggtatg tttttcaaat agagtatcac
     1261 caataggttc cccatactgc tgtgccagca gtgctgcacc ataggcttca aaatatgcag
     1321 cagtatctgg tatgataaaa tcaatatcag gcaatttttc tttaataaac tgtactatat
     1381 atggattacg ggtaatgcca ccagttataa tcactgtacc attgtgtata cgtgcttttt
     1441 ttaagaaatc tacaacttta actgccatca cattggaaag tgaaagaata atatcacctt
     1501 ttgttgcctc tcctttattg agcctgtggg tacagtcact tttcataaat actgaacagc
     1561 gcgttgataa ccgcaatacc ttgctttcat gtgggatgga atgaatatca gcaagcttca
     1621 tgttcatgcg tgccaattgt tgcttgaaga actcaccggt acccgcagca cacttgttcc
     1681 ctgaaaaatt attcttaatt tttttatgtt catcaacagt atagactaca aggtcctctc
     1741 ctcccataga tactattgca tctggcctgt aatcactatg tttcagcgct tcctcaatgc
     1801 aaacagattc tatgacacgg ggtaaatgta ataaaaacct tccttcattc ccggtaacta
     1861 tcgcccgtgc atctttggga atgtttaatt gcgatagaat tgttgtaagc gtgcccggca
     1921 catcaccttc atggggcatg atattaaatg tttcaacaac accactgtta cataccacaa
     1981 ctttaacact ggttgaacca atattaatgc ccacggttat cataacccta cctccacagg
     2041 aaaactggta ttgtcttttt gttatgcctc tgtcaatatt ttattttttt tatttatgtt
     2101 caggaatata gattgatgtt acaattgatg ctgtcaatac aaatgcaatt atcccaagcg
     2161 aaatttctgg ggatgtgata cccaacctga gcagcaatca tcttgcagcc tacatagatt
     2221 aaaataactg ataacccata atgcaaaaaa cgaaaaagac gcatgactgc tgccagcgca
     2281 aaataaagtg cccttagtcc cagaatagcc atgacattgg atgtatacac aataaacgga
     2341 tcatggctta ttgccagcac agcaggaatt gaatcaaccg caaacactac atctgttgat
     2401 tcaatgacca gcaagacaat aaaaagcggt gttacaaatc gtttcccatt ttctttaaca
     2461 aaaaaatatc cattgcgata gtcactggta acaggcagca tggtacgtgc taattttaac
     2521 actggatttt tttcaggatg aatttcttta tccttttctt tcatcatctt gtatccagta
     2581 tagattaaaa acgcaccaaa gatatacagt atccagtcaa acttttctat taatgctatg
     2641 cctgcaaaaa taaaaatggc acgcataatg agtgcaccca gtacacccca gaagagtact
     2701 ttgtgctgat acattggatg tactttaaaa tatgtaaaaa gcatcaggaa tacaaatata
     2761 ttatccatac ttaatgattt ttcaacaagg tatgcagtaa aaaacagcaa agctttatga
     2821 tatccggcgt aaaaatatat gcccagatta aacatcaaag acaacactat ccagaatatt
     2881 gtccacagta ccgcttcttt tacttttatt tcatgagcct tcctgttaaa gacacctaaa
     2941 tctaaaagaa gcataagcag gacaaaaaca ttaaaaccaa cccataatag agttttagtc
     3001 atctattggt actccacgtg cacagtacat catgtcaaag taaagcatcc gtaagcgctg
     3061 ccgaaagtag aaatgatgtc ccgtacatta ttatgttatt atactgttac ctgccaacct
     3121 tattttttat ccgcgagcgc agtgctttta aaaatccttc tattccttca ccggtttttg
     3181 cgcttacttt atgtacttcc atctttggat ttgtgcgttt aacatttgcc agtaattcat
     3241 ccatattgaa atccagatag ggcaacaggt ctattttatt aatcaataac gtatgtgaag
     3301 agttaaacat taacgggtat ttaaccggtt tgtcttcacc ttctgttaca ctcaatacca
     3361 ccactcgttc atcatcaccc aattcaaact cggctgggca taccagatta ccaacatttt
     3421 caataatgac cacatcaaca gcattaagat caaatttttc caggcacccc aatatccagg
     3481 agctttctaa atggcaggca ccgccaaata gctttgtttc tatctgaaca atgggaatat
     3541 tatactttgc caaacgctga gcatcattgg ttgtttcgat atcgccttca atgagggcta
     3601 tattgtaatc ctttataagg tcattgatta ttctatcagt aattgtggtt ttccccgcac
     3661 cgggtgaact catgatgtca aacatatata cttttttttg gctgagtaac ttttttattt
     3721 cctcagcctt ctgaatattt gattcataga tgttctgtaa tacctggatt tccattgtta
     3781 ttctcctata atttgaataa taatcaaagc aacaaaatta tagtgttcgt ttaaagttac
     3841 tttcaataat taaataaacc attttttttc aaataaaaaa attatatttc ccacacatca
     3901 aaaacctggc aaccctcaac catttctacc tttttatcta aagtaactat cgctattggt
     3961 atggatttat acatttgcat aaaaacagag tatttgctct ttctaaactg attttcatct
     4021 accttcactt cataggctat tttctcatta acaataaaat caacttcatt accctgtata
     4081 gttcgccaga actttatatt atcttcattg ttactatcca ccagttgtcg aaaacacgca
     4141 ttttcaagta gctgtccctt atcctttctt gttttaaaag actcaaaaga agaaacaaaa
     4201 aaatttctca gtccagtatc cataaaataa cacttgggca ttttggtcaa ttcttttcgt
     4261 acattcatat aaaaaggtct taccagggat atatgaaatg acttttgtaa tatatatata
     4321 taattatcaa ttgctgtctt tgaaatacct agagtatttg ctaactctga tgtatttacc
     4381 aatccaccaa tttgcgatgc taatatttta cataacttgt aaaaaacttc attacttttt
     4441 atatgtgctt catatatatc tttttttaca tatgaatacg ctatatccat taaaagatct
     4501 attttttgat caaaatcact caaaacaact tttggatatc caccatatag catatactca
     4561 tgataataaa atgatatttt ttccttttcg gttatactca gatttttaaa atcattatct
     4621 atcagtttat tctcattttt aaagcgtagg aattctttaa aagataatgt gcgcacataa
     4681 aacaactttt ttcgtccaac caacgaatca ttaaattttt tatcaatgta aaaagcactt
     4741 gaacctgata cacataattt tatttttccc ttatattcat cataaaaata ttttaaaaaa
     4801 tttgatggat tagtaagata ttgaatttca tctataaaaa cataactttt tttattcaaa
     4861 tcaaaactaa acagtttcaa aagatttttg ggattttcat taagtaaact taaataatct
     4921 ggatcttcca gatttataaa ataaactgca gtgctttctt tttcaagaga tttctggatc
     4981 tgccgtagaa tagttgtttt cccggtttgc cgagctccaa taaaaatcaa aatatcatca
     5041 ttatggatat agtttagtgc ttcaagggta acatctcgga taatgctcat aatcacttcc
     5101 tatattttat aaatacaaaa tataataaaa tttgtatctg tcaaatactt ttactataaa
     5161 tatcttatta tatttgaaca aaaaaaggct accaccttta taagatgata gccctttatt
     5221 atcctgttca atttcacaat attagcaaat cttgaaggtt cctatctcct tatccccttc
     5281 aacaacatgt accgcacagg ctatacatgg gtcaaatgaa cgaactacac gtgctgcttc
     5341 aattggattc ctgggatcag ttattggtgt acccaataac gcctgttcaa ctggtcctaa
     5401 cttaccatca tcaccccgtg gaccgcagaa ccatgttgtt ggtacaacgc actgataatt
     5461 tgctatcttc ttatcttcaa caacaatcca gtgtcccaat gcaccacgtg gtgcttcagt
     5521 caagccttca ccctggcctt tgtccggtat ttcatacgtt gagcgtggca tttcgccaac
     5581 ttccagatca tccagccact catacattct ctggcaaaga agtttacatt ctatagcacg
     5641 ggcagcatga cgtcccaatg tagagaatac tgcatcgata tcagcattga acactttgag
     5701 caatgcatca acttctttct ttacgtctgc attgccaccc agatagttca caactacgcg
     5761 tgctaatggc ccaacttcca ttggcatgcc atcatatcgt ggtgccttaa gccatgtata
     5821 tgcaccagct ttgtgtggat caggattagt ctgaccctgg tatggatgca ggttgctgcc
     5881 agagttgtaa cgagcaaacc gtacctgttc acgaatcttt gatgaatcaa attcttgtag
     5941 ttttttgcca tcaaaaactc cacgtttaaa gaagaactcc gtctgctctg gattttcttc
     6001 aaatacacca tacgacagga agtttgtata cctgccaatc ttgaaatagt cactgtatgc
     6061 ttttgccaca gctattacat cattgatata cacttcattt acaaattctt caacctgttt
     6121 taaaagtttt ctgtactgac gtatagcact gcgtgtgggc acctgcgtta caccgcctgg
     6181 tactaaacca ataagatgcg gcacccttcc tccaaacaga gcaaccatct gatgagcttt
     6241 cattctcata tcaagggcct gtacataatg agctatcgct gcaatattca aatcaagatc
     6301 tttaatgtaa aaatcgcctt catagcgtgg taaaaacggt gctacagctg taatagcatc
     6361 ctgtcggcct ttctttacct caagctcatt tttggcccaa tcacgcaacg caagaagctt
     6421 tttgtcagag ccattgtatt tcaggatagc agttatatcc acatagtcaa gtgcagctaa
     6481 atggtaaaaa tgcaatatgt gtgactgcag atagttggca ccaaatacta agtttctgag
     6541 cagtcgtcca tttttattgg gcactatacc aaacgctgca tctaaacatt tgcttgatgc
     6601 aataccgtgg gaaacagggc acacgccaca tatacgctgt gtgatctgat ttgcatcaac
     6661 agggtttcga ccttttaaaa tcatctcaaa accacggaac atatcacctt tactttttgc
     6721 atcaaccacc ctgc
//
LOCUS       DAOSZP010000037         6605 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_9873_length_6605_cov_3.247328, whole genome shotgun sequence.
ACCESSION   DAOSZP010000037 DAOSZP010000000
VERSION     DAOSZP010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6605
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9873_length_6605_cov_3.247328"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="PK348_01555"
     CDS             complement(<1..221)
                     /locus_tag="PK348_01555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK64928.1"
                     /translation="MIVNIIITISGIIFWIILLPVTIALISFKIVPTPHHITDLAGIG
                     VAVGIIGLLISLWAVISQITIGKGTPVPV"
     gene            complement(307..1008)
                     /locus_tag="PK348_01560"
     CDS             complement(307..1008)
                     /locus_tag="PK348_01560"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HXK64929.1"
                     /translation="MKKQWFIIISIILLVNIQASVVKQKQADTEKVKQQYVSDVERKD
                     AIIKKIHALMKEINANVGGEEKLVVPVEPSNATDVVDHDEYAKNEMPPDKKPEEDKKG
                     NKESLIAPTKGKMTSKYGYRENPVTKKYSFHKGIDIAAPKGTKVVASAHGIVASSQYM
                     NNGYGNCIIIEHENNIKTLYAHLEKRLVKPGEVVQQGQQIGTVGATGRATGPHLHYEV
                     YKGKQSINPEELIHY"
     gene            complement(1237..4440)
                     /locus_tag="PK348_01565"
     CDS             complement(1237..4440)
                     /locus_tag="PK348_01565"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HXK64930.1"
                     /translation="MPDKKSLVTLILSIFFLWAIVTEVFASNTLFVTDDFDVANLGLY
                     VQYLRDVPPHTDISEIITAYNNNQFKESTDINPSFGYSHNCIWVSFTLANTSKTPVPL
                     LLEYDYPIIDDITLFIPGGVHFKEIQTGDRFPFSSRPFPYRSFVFPIEVKPGVNTYFL
                     RIQSEGSLVVPLKLWGLKAFEDMRNSTLPLIWMHYGLMVALFFYNLFLFISTREKSFL
                     YVGIFIIGVTLFAMANNGLAAQYLWPTSTWWGNICHPFSVFFAMTGILLFTREFLSLR
                     FVIRKLDTYVLLLTLITVLSALTPFLLPYYYATQLSVLMAAIGSVSVIVIGVVAQIKK
                     IREAIFYNIATLSFVIGVLLIGLRSFGVLGDNFFTAWGYQLGSSAMIILFSLGIADKV
                     NTWRRDKERALSQLATSEARYRTLVENAHDGIILVQNWKIVYSNDAFARMIGYTTQEL
                     ENLNLKNIVADTELGKEVLTQYNNRLQKKEAKLQYEAQLKRKTGNIIDVLISAQMVSL
                     DGIPTSIAIITDISELKEAYDTIQQQYEEIQSQYEELEAMNEEMVRTHYELTEATHKL
                     EEETERLHATLRSIYDGVVATDLQGHIILANDKAEKLAGKSSDEVIGRPFNSVFTFYY
                     VTTREEFKNPVNEILQYGELDTSGFALTFKSQDRDVVVELSGAPIYVEGNIIGTVIVM
                     HDITGQYWLEKEIQKVSKLESLSILAGGIAHDFNNLLTSIIGNLSILKVKLTDGELYQ
                     KYIERIENAAQRAADLTRQLLTFSKGGEPIKEVTSLEQVIRDTAQFVLSGSSVRCNFE
                     IQSDMWNCEVDVGQMSQVFQNLIINAAQSMPHGGIITIKAENFVYDGTQQKPLLPGNY
                     VRITVKDEGIGIPKENLSRIFDPFFTTKERGSGLGLATSYSIIKRHGGHIEVHSEVDK
                     GSKFIIYLHATEKSIHSHTGQEAHITPPGGKILLMDDDFTIYEVASHMLDMYGFQVDW
                     VDSGEKAIIKYRESIADGFPYDIIIMDLTVPGAMGGVQAVREILEINPKAKVIVSSGY
                     SNDPVMSHYKEYGFKGVIAKPYKIEEIVRVINEVLSQ"
     gene            4604..4744
                     /locus_tag="PK348_01570"
     CDS             4604..4744
                     /locus_tag="PK348_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014454461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK64931.1"
                     /translation="MAHYKLDGAKFESLEELKEVMWQLYKDKMSREEFEKYVEQNVQV
                     SE"
     gene            complement(4729..5505)
                     /locus_tag="PK348_01575"
     CDS             complement(4729..5505)
                     /locus_tag="PK348_01575"
                     /inference="COORDINATES: protein motif:HMM:TIGR01486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HXK64932.1"
                     /translation="MDAFPVVFSDIDGTILDYTTYSYKKSLPAIQKLAGRGIPLILVS
                     SKTYAEMLKLHKELSLTYPFVFENGAGIAKPDGSYILCGKSYDQLLPYKTIIEQLCGN
                     VQWADSLSLQELSAYTGLTIQETKNMMARMASVLCIAPAPLDIERINNELIPHGIAIT
                     TGGRFFTVIDSTVNKGNAVHVIKAMYREKHGAIYSYAIGDGINDADMFNAVDEAYFVG
                     RKDLWGTIQKLCPVIHKTKKTGPSGFSEVIKNIIKNYSLT"
     gene            complement(5508..>6605)
                     /locus_tag="PK348_01580"
     CDS             complement(5508..>6605)
                     /locus_tag="PK348_01580"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HXK64933.1"
                     /translation="GLYMYQIQCTDNAGNKAQAMIREISLTRQYQTVDVSASLEYFSH
                     ALHNTIVFTPIVSDTKDLVKWQLVVTNEKDKPVYQTQGQVMPKTITWDCKTDKGEKLN
                     DGIYRYKFSAEYLSGNNPQSFVKTFTIDSTPPDVNLSYEPDLFSPDGDGENDILTITP
                     SINDNVGIKSWELIIYAPAGYVFKTFKGTEVPGELKWDGIGVNNELVESAADYFMELS
                     ATDITGNVAKTKRIKLPIDVLVMVTERGLKIRISNIEFAFDSAKLTGKAFPILNRVAE
                     ILNKYEKYNVLIEGHTDDIGEEQYNLKLSEARAQSVMDYLVKKGIDKKRLSSRGMGET
                     SPFLPNTSVENRRRNRRVEFLLIKPEESQVN"
BASE COUNT         1937 a         1281 c         1172 g         2215 t
ORIGIN      
        1 aacacaggga caggtgttcc tttacctatg gttatttgtg agataacagc ccatagagaa
       61 attaacaggc caatgatccc cactgcaaca cctatacctg caaggtcagt tatgtgatgc
      121 ggtgttggta caattttaaa tgatattagt gcgatagtta ccggtagtag tataatccag
      181 aaaataatcc cggatattgt tataataatg ttaacaatca cctctcataa tcctttcaat
      241 aaaaaatctt caggcataaa aatgtatttc ctttgaacat gtgagatagt aatcataagt
      301 aaaaagttaa tagtgaataa gctcttctgg atttattgac tgcttgcctt tatacacctc
      361 atagtgaaga tgtggtccgg ttgctctacc ggtagcacca acggtgccta tctgttgacc
      421 ttgttgcaca acctcacctg gctttaccag acgtttttca agatgtgcat acagtgtttt
      481 tatattgttt tcatgttcaa tgatgatgca attaccatat ccattattca tgtactgtga
      541 tgaagcaaca atgccatgtg cacttgccac tactttagtc ccttttggtg cagctatgtc
      601 tatcccttta tgaaatgaat attttttggt tacaggattt tccctgtatc catatttact
      661 tgtcattttt ccttttgttg gtgcaattaa ggattctttg ttgccttttt tgtcttcttc
      721 tggttttttg tcaggtggca tttcattttt tgcatattca tcatggtcaa ccacatcagt
      781 agcgttagaa ggctcaacag gaactaccag tttttcttct ccaccaacat ttgcatttat
      841 ttctttcatt aatgcatgta tttttttaat gattgcatcc tttctttcaa catcagatac
      901 atactgctgt tttacttttt cagtgtcagc ctgtttttgc ttaactacag aagcctgaat
      961 atttaccaat agtattatgc ttataataat gaaccattgt tttttcatat atacattaaa
     1021 tctccttgta aaatagaatt tcatgattgt catccctgat actatccgta atctttggag
     1081 cagtaagatt gtgaatcacg ttcaggatga ccattgcaaa aaaatatttt catttcacga
     1141 ttgcagagta acaccctgta tccagataca gttatctgct atcacggcga tatatattca
     1201 tactatattt attttcggag ttattaaagg aaagtcttac tgtgaaagga cttcattaat
     1261 tactcttact atctcttcaa ttttatatgg ttttgcaata acacctttga atccatattc
     1321 tttataatgt gacatcaccg ggtcattgga atatccgctt gaaacaataa cttttgcttt
     1381 aggatttatt tctaaaattt ctcttactgc ctgtacccca cccatggctc cagggactgt
     1441 aaggtccata atgataatat cataaggaaa gccatcagct atggattcac ggtacttaat
     1501 aattgctttt tccccactat caacccaatc aacttgaaat ccatacatat caagcatatg
     1561 cgaagcaacc tcatagattg tgaaatcatc atccatgagc agtatttttc ctcctggggg
     1621 agtaatgtgt gcttcctgtc ctgtgtggct atgtatgctt ttttctgtag catggagata
     1681 gataataaat tttgatcctt tatctacttc tgaatgaact tcaatatgtc caccatgacg
     1741 ctttattata gaataacttg ttgccagccc taaaccactg ccacgttctt tagtggtaaa
     1801 aaaaggatca aatatccgtg ataaattttc cttcgggata ccaattcctt cgtcttttac
     1861 tgttatcctg acataattgc caggcaacag tggtttttgc tgagtaccat catacacaaa
     1921 attttcagct tttatggtaa taatccctcc gtgaggcatg gattgtgctg catttatgat
     1981 taaattttga aacacctgac tcatctggcc cacatctact tcacaattcc acatatcaga
     2041 ttgtatttca aaattacacc ttacactgga acctgacagt acaaactgtg cggtatccct
     2101 gattacctgt tccagcgaag ttacttcttt tattggctca ccaccttttg aaaaggttaa
     2161 aagctgtctg gtgaggtcag ctgccctttg tgctgcattt tcaatgcgtt ctatatactt
     2221 ttgatataat tctccatcag taagtttaac tttcagtatt gaaagattgc caattataga
     2281 tgttaataga ttattgaagt catgagcaat gcccccagct aaaatagaaa gtgattctaa
     2341 tttacttacc ttttgaattt ctttttccag ccagtactga ccggtgatgt catgcattac
     2401 aataactgta ccaatgatat ttccttcaac gtagattgga gcacccgata gttctacaac
     2461 tacgtctcta tcttgggatt taaatgtcag agcaaagccg cttgtatcaa gctcaccata
     2521 ttgtaaaatc tcatttacgg gatttttgaa ttcttctctg gtggtaacat aataaaatgt
     2581 gaatactgaa ttgaatggcc tgccaattac ctcatcagat gatttgcctg ctaatttttc
     2641 agccttgtca ttggcaagaa taatatggcc ctgtagatca gttgctacaa caccatcata
     2701 tatagaacgc aatgttgcgt gtaatcgctc agtctcttct tcaagcttat gcgtagcttc
     2761 agtaagttca taatgcgtgc gcaccatttc ttcattcatt gcttccaatt cttcatactg
     2821 agattgtatt tcctcatact gctgttgtat ggtatcatac gcttctttca attcgctaat
     2881 atcagtgata atggctatgc tggtgggtat gccatccagg gaaaccatct gagctgaaat
     2941 gaggacatct attatattgc cagtttttcg ttttagctgt gcttcatatt gcagttttgc
     3001 ttcctttttt tgcaatcgat tattatactg ggttaatacc tctttaccca actcagtgtc
     3061 agccacaata ttctttaaat ttaaattttc aagttcttgc gtagtatacc caatcatccg
     3121 tgcaaatgca tcattggagt agacaatctt ccagttctga accagaatga ttccatcatg
     3181 ggcgttttca acaagggtac gatatcgagc ttctgaagtt gcaagttgtg acaatgctcg
     3241 ctctttatca cggcgccacg tatttacctt atcagcaatc cctaatgaaa acagtataat
     3301 cattgccgat gagcctaatt gataacccca tgcagtaaag aaattatcac ctaatacacc
     3361 aaaagatcgt aatccaataa gcaacactcc tattacaaag cttaatgtgg caatattgta
     3421 aaagattgcc tctctgattt tttttatttg tgctactact ccaataacaa taactgaaac
     3481 tgagccaata gctgccatga gtactgatag ctgtgtagca tagtaatatg gcagcaaaaa
     3541 tggtgttagt gcactaagta cagttatgag tgtcaataga agtacatatg tatccaattt
     3601 tcttataaca aatcgtaatg ataaaaattc acgggtaaag agcagtatgc cagtcattgc
     3661 aaaaaataca gaaaaaggat gacatatatt tccccaccag gtacttgtag gccacaaata
     3721 ttgagcggca agtccattgt tggccatggc aaaaagggtt acgccaatga taaatatacc
     3781 tacatacaaa aagctttttt cacgagtgga tataaataaa aatagattat aaaaaaataa
     3841 tgcaaccatc agaccatagt gcatccatat tagtggtaat gtactattgc gcatgtcttc
     3901 aaaagctttt aatccccaca gttttaaggg tacaaccagg gatccttcag attgtattcg
     3961 aagaaaatat gtgttaacac caggttttac ttcaatgggg aatacaaatg atctgtaagg
     4021 aaaaggacgt gatgaaaatg ggaaacgatc acctgtttgg atttctttaa aatgcacacc
     4081 accgggaata aaaagggtaa tatcatcaat aatggggtaa tcatactcta aaagcaatgg
     4141 tacaggtgtt tttgatgtat ttgcaagggt aaagcttacc catatacagt tgtgagaata
     4201 gccaaacgat ggatttatat cagtagattc tttaaattga ttattgttat aagcagtgat
     4261 aatttcgcta atatcagtgt gtgggggtac atcgcgtaaa tattgtacat acaatccaag
     4321 attggcaaca tcaaaatcat cagtaacaaa tagtgtatta ctggcaaaaa cttcggtaac
     4381 tatcgcccac aaaaaaaata tggataatat caatgttact aagctctttt tatctggcat
     4441 tgttgtacac cctgcaaacg atgatactgg tatttgtgat ttaattcaaa tgttttttta
     4501 ggataattgt aacattatgg tgtaaaattt tatattgaca gtatagttgt aaagtgttta
     4561 ttggtattta agatttgtat attcagcaaa agggttctgt agtatggcac actataagct
     4621 tgacggggca aaatttgaat cgcttgaaga attaaaagaa gtcatgtggc agttgtataa
     4681 ggataaaatg tcccgtgaag aatttgaaaa gtatgttgaa cagaatgttc aggttagcga
     4741 ataatttttt attatatttt ttatcacctc actaaaacca gatggtccgg ttttttttgt
     4801 tttatgtatt acagggcata atttctgaat ggttccccac agatcttttc ttccaacaaa
     4861 ataggcttca tcaacagcat taaacatgtc tgcatcattg atgccatcgc ctatcgcata
     4921 ggagtatatt gcaccatgct tttcccggta cattgctttt atcacatgaa ctgcgttgcc
     4981 tttattaaca gtgctatcaa taacagtaaa aaacctccca ccggttgtta tggctatacc
     5041 atgcgggata agttcgttgt tgattctttc aatatcaagt ggggcaggtg ctatacataa
     5101 caccgatgcc atgcgtgcca tcatattctt tgtttcttgt atggtaagcc cggtatatgc
     5161 cgatagttcc tgcaatgaaa ggctatctgc ccactggaca ttaccacaca actgttcaat
     5221 aatcgtttta tacgggagaa gttggtcata tgattttcca cataaaatat aacttccatc
     5281 tggtttggca ataccagcgc cattttcaaa gacaaatgga tatgtaagcg aaagctcttt
     5341 gtgcagcttc aacatttcag catacgtttt gctggaaact aaaataagag ggatgcccct
     5401 gccggcaagc ttttgaattg ctggcaggga ttttttatat gaatatgtgg tgtaatccag
     5461 tatggtcccg tctatatcag aaaatacaac agggaaagca tccatggtta attaacctgt
     5521 gactcttcag gtttaatcaa cagaaactca acacggcggt ttcgccttcg gttttcaaca
     5581 cttgtgttag gtaaaaatgg tgatgtttcc cccatgccgc gtgaagataa gcgcttttta
     5641 tcaatacctt ttttgaccag ataatccata accgattgag ctcgcgcttc agaaagtttc
     5701 agattatact gttcctcacc tatatcgtca gtatgccctt caatcagaac attgtatttc
     5761 tcgtatttgt tgaggatttc tgcaaccctg tttaaaattg ggaatgcttt cccggttagt
     5821 tttgcactat caaacgcaaa ttcaatgttg ctgatgcgaa tcttgagtcc acgctctgtc
     5881 accataacca atacatcaat gggtagtttt atgcgttttg tctttgcaac atttccagtt
     5941 atatcggtgg ccgatagttc catgaagtaa tccgcagcag attcaacaag ctcattgttt
     6001 accccaatgc catcccactt gagttctccc gggacttcag tgcctttgaa tgttttaaat
     6061 acatatccag ctggtgcata gattatcaat tcccagcttt tgattccaac attatcgtta
     6121 atagatgggg tgatagtaag aatatcattt tccccatcac catcaggcga gaaaagatca
     6181 ggttcatagg atagattaac atcaggtgga gtgctatcaa tggtaaacgt tttcacaaat
     6241 gattgtggat tattgccact taaatattca gcactgaatt tgtagcgata gatgccatca
     6301 ttaagctttt cgcctttatc tgtcttgcag tcccaggtta ttgtttttgg catcacctga
     6361 ccctgagtct gatatacagg tttgtccttt tcattggtaa caacaagttg ccatttaaca
     6421 aggtctttgg tgtcagagac tataggagta aatactatag tattgtgtaa tgcatgtgaa
     6481 aaatattcca gtgatgcgct gacatcaact gtctggtact ggcgagttaa acttatttcc
     6541 ctgatcatag cctgtgcttt gttgccagca ttgtcagtgc actggatctg atacatgtac
     6601 agacc
//
LOCUS       DAOSZP010000038         6587 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_9921_length_6587_cov_3.273270, whole genome shotgun sequence.
ACCESSION   DAOSZP010000038 DAOSZP010000000
VERSION     DAOSZP010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6587
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9921_length_6587_cov_3.273270"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            322..1311
                     /locus_tag="PK348_01585"
     CDS             322..1311
                     /locus_tag="PK348_01585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK64934.1"
                     /translation="MQLKKEIKTITLKDPFQQFKEAQRVNEIRYCPLTGKSTRVLGLS
                     LKEFATADRSAMIEESQKTCPFCPGLIDALTPKFTPPLQEQYTSGNARCFPNAFPYDE
                     NNAVVTITSQHYVPVNGFTHQMLQEALECALIFLSDIDPERYPYQSINWNYMPVAGSS
                     LVHPHFQIIASSTPSNYYVDILANLEKNPNLFMDYLNTEKSLNERLIEVMGDSTWLIA
                     FAPRGQFDILGILHECTPLHTLTGKLDSVITYIRKTLQFFHHKKIDNFNMAFYSIANN
                     KLFLPHIRLCPRVTFPPYATSEINYLQMLHNDSFTLLKPEIITEEFKAFQFDL"
     gene            1689..2648
                     /locus_tag="PK348_01590"
     CDS             1689..2648
                     /locus_tag="PK348_01590"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HXK64935.1"
                     /translation="MLQKVLQEYDSKIHLEELLTFGMALFLNILIIVFSHITLAPVIF
                     TINTIIMLLIFATVYAQRYATSTIFTIYRDWYILPLLVAIFMEHNKLVPLINPHDIDT
                     ALIKIDYALFFGNHPTVLLENVLNPFITELLQIVYASFYFLPFTLCLLLYLKERKVEF
                     HIVASTIMLGFYLSYIGYYIFPAIGPRFTLTHLQTKPLEGLWLFNFVHHGLAYLEGVT
                     RDCFPSGHTLVSILTVLLAIKFHKKFSVTATVWALLLIISTVYLRYHYVIDVIAGFFV
                     GLATFRYGPVLARLYIFGSAEISTPVLDYLRNWWDWLSEIRRR"
     gene            complement(2655..3383)
                     /locus_tag="PK348_01595"
     CDS             complement(2655..3383)
                     /locus_tag="PK348_01595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK64936.1"
                     /translation="MRRRIKTKPVTIPETKKTYWKRRPSEIRGYQRVFYCNSENGDYI
                     RVDYNFKDKRVRLYLEDSEEGGNPYYAVIDKGRISAERNATSGRPVSLQEKFEKRAEL
                     ISTIPNKDVIKLINKHYGIGESPEEIAARKKKRQEELERTRKRYFKPEEVPSFVKKDT
                     TVVSKQRVRIRDMMDIGIGGATVGGVYFVSLDFIKAGIVAAAFGILLAFVDIFIREKE
                     TLLLKTLLFLVLGIALYIYGYFIL"
     gene            complement(3649..4533)
                     /locus_tag="PK348_01600"
     CDS             complement(3649..4533)
                     /locus_tag="PK348_01600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK64937.1"
                     /translation="MGKVQKIIIAAFIIAALVYIFIRSVVFVGDGVLAFVTDTNGQIL
                     GHLHKRVNLVPQGILVTIKANYIPLHGSCNVDIVIPISPLESIKSDHYSIKIPVVINY
                     TLNPEQLTLDLSRLYSDTSYLSQQFEIRLSGSMKDAISRYLYPYYQYNALQVNIHKEL
                     SVAIDHVKESFARDGIIIKEVTPGIIYVPDYTVYTEGKKFLLELLTQEKLRTIQLNAL
                     QQKLKEDELKNAQYLEHLEKISRIIAKNKDILKYIYIDKLADNVRVIVTSDKNIPLDI
                     DSDSESESLKKDFDNFKK"
     gene            complement(4537..4767)
                     /gene="hfq"
                     /locus_tag="PK348_01605"
     CDS             complement(4537..4767)
                     /gene="hfq"
                     /locus_tag="PK348_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001274793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HXK64938.1"
                     /translation="MGKQTKNLQDTFLNAARKDKVEITIYLMNGVPIKGKVISFDSFT
                     ILVEVDKKQNLIYKHAISTIMPSKPVQYKDEE"
     gene            complement(4892..5803)
                     /gene="miaA"
                     /locus_tag="PK348_01610"
     CDS             complement(4892..5803)
                     /gene="miaA"
                     /locus_tag="PK348_01610"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HXK64939.1"
                     /translation="MNNKAVVLVGPTASGKTNLSLEIAQDNFEIVSVDSAQVYTYMDI
                     GTGKVSPYHRSLITHYCIDIVNPDYWFTAGDFCREAEHAIQIIQSHGKIPLFVGGTGL
                     YIHAYFYGLAELPKIDSTVKKQLQSELEQKGLKTLYEELLHCDLQWAKRIHPNDTQRI
                     LRGLEVYRATGIPITQFLQSGHKKSNLNILMIALDVDRDVLYKRIENRVDEMIRQGFV
                     EEVKNLRAMGYTQELNSQKAIGYLQLHRVLTNELSLNEAIEQIKQETKHYAKRQVTWF
                     KRYNPVWVAPDNIERIKGIISEWLYKK"
     gene            complement(5894..6094)
                     /locus_tag="PK348_01615"
     CDS             complement(5894..6094)
                     /locus_tag="PK348_01615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK64940.1"
                     /translation="MIIPLDKLIAFEDNRYVFSCAAMKVVDKLGNIEGYPESDKNWKV
                     VPNILNLMLNNTIKYEFKEETK"
     gene            complement(6075..>6587)
                     /gene="gmk"
                     /locus_tag="PK348_01620"
     CDS             complement(6075..>6587)
                     /gene="gmk"
                     /locus_tag="PK348_01620"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR03263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HXK64941.1"
                     /translation="TRVKRAGEAEGKNYYYVSYSEFMQMVDRGDFLEWAKVHDNYYGT
                     TKKEVDRIKANSRIPIFDVDVQGARSLRNKLQNVISVFIIPPSIKELERRLRHRSTET
                     DHQITVRLNNAIGELREYVNYDYIVVNDDLQKALDELNAILKTIILKREYMDSVVKRL
                     LEDVHDYTIR"
BASE COUNT         2026 a         1280 c          964 g         2317 t
ORIGIN      
        1 tatagtaata gtagttatat ttttttatta gtgcaacaag ctcatcatag cgctttttta
       61 ctttttccat tgttatcacc ccttctaaaa acgcaatttt gaataaagta ttatcctgat
      121 taacgaaatt atgaaagatg gcagcattgc catgttgttt taatataaag ttttatgtaa
      181 ctccaatcaa caaagttcat ttgccaagat aaaaaaagta gtttaacacc atgtaagata
      241 aaaaaattat tgtaaaatac attagacgtt ttaaaaagca aaatagtatt tagattatgt
      301 attaatcaat gggggaaaag tatgcaacta aaaaaagaaa tcaaaacaat aacactgaaa
      361 gacccattcc agcagtttaa agaagcacag cgagttaatg aaatacgata ttgtccatta
      421 acaggcaaaa gtactcgtgt tttaggttta tccttaaaag aatttgctac tgctgaccgc
      481 tctgcaatga ttgaagaatc acaaaaaacg tgcccctttt gccccgggct tattgatgct
      541 ctgaccccaa aatttactcc accactacag gaacaataca ctagtggcaa tgcccgatgt
      601 tttcccaatg cgtttcctta tgatgaaaat aatgcagtgg tgaccataac cagccagcac
      661 tatgtacccg ttaatggttt tacccatcag atgttgcagg aagccttaga atgcgcactg
      721 atctttttgt ccgatattga ccctgaacga tatccctatc aatcaataaa ctggaattat
      781 atgccagttg caggcagctc ccttgttcat ccgcacttcc agatcatagc atcttccaca
      841 ccatcaaatt attatgttga tatactggca aacctggaaa aaaatcccaa tctttttatg
      901 gactacctta acactgaaaa aagcctgaat gaacggctta tagaagttat gggcgatagt
      961 acctggttaa tagcttttgc tccaaggggt cagtttgaca tacttggcat actccatgaa
     1021 tgtacaccac tacatacatt aacaggaaaa ctagattcgg taattacata tatacgcaaa
     1081 acattgcagt ttttccatca taaaaaaatt gataacttca atatggcttt ttacagtatt
     1141 gcaaacaaca aattattttt accgcatata cgactatgcc cacgtgttac atttccacca
     1201 tatgcaacaa gtgagatcaa ctatctccag atgttgcata atgattcatt cacattgctc
     1261 aagcctgaaa taattaccga agagtttaag gcatttcaat ttgacttata agtgaacttc
     1321 taaaaactgt ttttttagga tgttccctta tgggcacaat agatgggata aaaccgcttt
     1381 ctttttattg catccgtaat gatgaaagcg gttttttaga ggtgcccata atatttcttg
     1441 acaaatgatt aaaattctga ccaaaattgt tcttgtctgg catttatcta tacaacaggc
     1501 tttatcaggg aaggtaatgt gtattcctga tgattgaaga aagtacaata gtattaaatc
     1561 gtaaatattt agtttttgta taatatttca tgcaaaatat tgtcatttta taacaaattg
     1621 tatatattat tttataagat aatagaaaac tataccatac atacatatat tgggataaga
     1681 ggccggatat gctacagaag gtgttacagg aatacgattc aaagattcat ctggaagagt
     1741 tgctgacatt tggcatggca ctttttctta atatattaat tatagttttt tcgcacatta
     1801 cgctggcacc ggtaatcttt accatcaata ccatcattat gttacttatt tttgcaacag
     1861 tatatgctca gcgttatgcc acttccacaa tatttactat ttacagagat tggtatatat
     1921 tgccacttct tgtagcaata ttcatggagc ataataaact ggtacccctt attaaccctc
     1981 atgacataga tactgccctg attaaaattg actatgcact attttttggt aatcatccta
     2041 ccgtattact cgaaaatgtc ttaaatccct ttataactga gctattacag atagtttatg
     2101 caagtttcta ttttttacca tttacactat gcttattatt atatcttaaa gaacgaaaag
     2161 ttgaatttca catagttgct tccacaataa tgctcggatt ttacttatcg tatattggct
     2221 actatatatt cccggctata ggcccaaggt ttacactgac tcatcttcag acaaaaccac
     2281 ttgaagggct atggttattc aactttgtcc atcatggact ggcatattta gaaggtgtta
     2341 ctcgcgattg tttccccagt ggccacacat tggtatcaat tttgacagta cttcttgcca
     2401 taaaattcca taagaaattt agcgttactg caacagtatg ggcactactt ctgattatat
     2461 caacggtgta tctgcgctat cactatgtca ttgatgttat agcaggattt tttgttggcc
     2521 tggctacatt tcgatatggc cctgtactgg cacggttata tatatttggc agtgcagaaa
     2581 tttctactcc agtactggat tatttacgca actggtggga ttggctgtct gaaattagaa
     2641 gacgttaact ttaattataa aataaaataa ccatatatat acaaagctat tccaagaact
     2701 aaaaacaata atgtcttgag caatagtgtt tctttttcac gtataaaaat atctacaaat
     2761 gccagaagta ttccaaaagc tgcagcaaca atacccgctt taataaaatc taaactgaca
     2821 aaatacacac caccaacagt tgcaccacca attccaatat ccatcatatc acgtattcta
     2881 actctttgtt ttgaaacaac tgtggtatct tttttaacaa acgatggtac ttcttctggt
     2941 ttgaaatacc gttttcgggt tctttccagt tcttcctgtc gttttttctt tctagcagct
     3001 atttcctcag ggctttctcc tattccataa tgtttgttta taagttttat gacatcttta
     3061 ttgggaattg tggatataag ttcagcacgc ttttcaaatt tttcctgcaa acttacaggc
     3121 cttcctgatg tagcattcct ctcagcagaa attcgaccct tatctatcac tgcataatag
     3181 gggttcccgc cttcttcact atcttctaaa tagaggcgaa cccttttatc tttaaaatta
     3241 taatcaaccc gaatataatc tccattttca ctattgcagt aaaaaacgcg ctgatagccc
     3301 cgtatttctg atggccttcg tttccagtag gtcttctttg tttcagggat tgtaaccggt
     3361 tttgttttta ttcttctacg cattatataa cctgattata tagtaaaata gaaaagagat
     3421 tgacctgtcg ttttgccaat ctctcttcca tttacattat cgtaatttct tcttgtgacg
     3481 attaagtctt cttcgttttt ttcgtttgtg attagcaatc tttctgcgtt ttcgcttcct
     3541 accacagggc ataatattta actctcctta tataaaaaat atatcaatat aatactttat
     3601 gaagtatatc caattattaa tatactgcga aattaagtaa ataatccact attttttaaa
     3661 gttatcaaaa tctttcttta aagattctga ttcgctgtca ctatctatat ccaatgggat
     3721 atttttatcc gaagttacta tcaccctcac attatcagct aatttgtcaa tataaatata
     3781 cttcaatata tccttatttt tagctataat ccttgatatt ttttccaggt gttccagata
     3841 ctgtgcattt tttagttcat cttccttaag cttttgttgc aatgcattta attgtatagt
     3901 acgtaatttc tcctgggtaa gcaattcaag caaaaatttc ttaccctccg tataaacagt
     3961 ataatcaggc acatagataa tacctggagt aacctcctta ataataatac catctctggc
     4021 aaaggattct ttcacatgat ctatcgccac agatagttct ttatggatat ttacctgcaa
     4081 ggcgttatac tggtaatacg gatatagata tctggaaata gcatctttca tgctccctga
     4141 caaccgtatt tcaaattgct gtgatagata tgacgtgtcc gaatacagcc tacttaaatc
     4201 cagtgttaat tgttcaggat tgagtgtata attaataaca actggaattt taatagaata
     4261 atggtcactt tttattgatt ccagcgggga gatagggatt acgatatcca cattgcatga
     4321 accatgtaag ggaatatagt ttgccttaat ggtaacaaga atcccctgtg gaaccagatt
     4381 cacccttttg tgtaaatgtc caagtatctg accatttgta tcagtaacaa aagccaatac
     4441 tccatcacca acaaagacaa cagatcgtat aaaaatataa accaaagcag ctataataaa
     4501 tgctgcaata ataatttttt gcaccttacc catgaattat tcttcatctt tatactgaac
     4561 aggctttgac ggcataattg ttgatattgc atgtttataa ataagattct gctttttgtc
     4621 aacctcaact aaaattgtga agctatcaaa gcttatcacc tttcccttga ttggaacacc
     4681 attcataagg tagatagtaa tttccacttt atctttgcgt gctgcattta gaaaagtgtc
     4741 ctgtaaattt tttgtttgtt tgcccattgt gcaccctctt tatgtatttt aatgttaata
     4801 tttattttat tgtaactatt tgttactact taacacttac ttatcattga atagtaataa
     4861 tatcaatatt tttttctaat tattttaata tctacttctt atatagccat tcagagatta
     4921 ttccctttat tctttcaata ttatctggtg ctacccatac tggattgtat cgcttaaacc
     4981 aggtgacctg ccgtttggca taatgctttg tttcctgttt tatttgctca attgcttcat
     5041 taagcgatag ctcatttgtt aacaccctgt gaagttgtaa atatcctatt gccttttgag
     5101 aattgagttc ctgtgtatat cccatagctc ttaaattttt tacttcctcc acaaagccct
     5161 gtctgatcat ttcatcaacc ctgttttcaa tccgtttgta taatacatca cggtccacat
     5221 ccagcgcaat cattaaaata tttaaattac tctttttatg ccctgattgt aaaaattgtg
     5281 ttattggtat accggttgct ctgtaaactt ccagaccacg caatatacgc tgagtatcat
     5341 tgggatggat gcgctttgcc cattgtaaat cacagtgcaa tagctcttca taaagggttt
     5401 tcagcccttt ttgctctaat tcactttgca attgtttttt tactgttgaa tcaattttgg
     5461 gcagttcagc taacccataa aaataagcat gaatatacaa tccagtacca ccaacaaaaa
     5521 gcggtatttt cccatgagat tgtataatct gtattgcatg ctcagcttct ctgcaaaaat
     5581 ctcctgcagt aaaccagtaa tccgggttaa caatatctat gcaataatgt gttatcaaag
     5641 atctatggta gggggaaact tttcccgtac caatatccat atatgtataa acctgagctg
     5701 aatcaacaga aactatctca aaattgtcct gtgctatttc aagcgataga tttgtttttc
     5761 ctgaagctgt tggaccaaca agaacaacag ctttattatt caaatcattt atcccaaaac
     5821 atcaaaacct gtatgcatta tcatcaacaa tttaagcatg atgtcgtatc agaattggca
     5881 agcactattg ccgttattta gtttcttctt taaattcata tttgattgtg ttattaagca
     5941 tcaaattcaa tatgtttgga acaaccttcc agtttttatc actttctgga tagccttcaa
     6001 tattgcccag cttatccacc actttcattg ctgcacagga aaatacatat ctgttatctt
     6061 caaatgctat taatttatct aatggtataa tcatgtacat cctccagtaa cctttttact
     6121 acagaatcca tatattcacg tttaagtata attgtcttta aaatagcgtt gagttcatcc
     6181 agggcttttt gtaaatcatc attaactact atataatcat aatttacata ttcccgcagt
     6241 tcaccaattg cattatttaa tctcaccgtt atttgatgat ctgtctctgt tgaacggtgc
     6301 ctcaaccttc tttccagttc ttttatagat ggtggaataa taaatactga tataacattt
     6361 tgtaatttat tacgtaagct tctggcaccc tgaacatcca catcaaaaat aggtattctt
     6421 gaatttgctt taattctgtc aacctctttt ttagtggtac cataataatt atcatgaacc
     6481 ttggcccatt ctaaaaaatc acccctgtcc accatctgca taaattcact ataacttaca
     6541 taataataat ttttcccttc agcctcacct gcccgtttaa cccgggt
//
LOCUS       DAOSZP010000039         6558 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_9977_length_6558_cov_3.537906, whole genome shotgun sequence.
ACCESSION   DAOSZP010000039 DAOSZP010000000
VERSION     DAOSZP010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6558
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9977_length_6558_cov_3.537906"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..454
                     /locus_tag="PK348_01625"
     CDS             <1..454
                     /locus_tag="PK348_01625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK64942.1"
                     /translation="FHTMLYSVVCMYNDILDDSAIARTLLAATQDTFYYHMMYFQDTY
                     DRLPVRIFQLFETLETFLHAQEHYACINPILLVTPDNYTRISQKDFAYPFPIVIQKGF
                     ARHCINWLKQQYSESIELVSFQDEADQKYVYNELGIEYVKIVFNTYFP"
     gene            1072..1524
                     /locus_tag="PK348_01630"
     CDS             1072..1524
                     /locus_tag="PK348_01630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK64943.1"
                     /translation="MNIETTTCIAYENVKLLQDYADVYKLSLHTFIINFITYVMSYKK
                     IPVKSCRRLTYRKRYERWKRVHLYLYENEYEFLMDVRKVCKMSIAKVIAYCIENYLFD
                     FLAALDSEDNTDNYRFSEYTFMFYLENGIQCCRFYWGPHPELVKLAMQ"
     gene            complement(1959..2579)
                     /locus_tag="PK348_01635"
     CDS             complement(1959..2579)
                     /locus_tag="PK348_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK64944.1"
                     /translation="MYILRENFYKLLTNLIITIFFIFACDEPSHGHGKNELWYYPDCY
                     TIQTAGYIGFIAMGIGFLSFNNTWESSLLYGFSPADVTGKEIHTVSWKNIVYPFTLNF
                     LDDNVLLPFYFGVTMLFVMDKNVYWERSTNCPDRYYNPTGRHSAVSVGLQIQGKSTMI
                     GLIHGFYVEITILDTYLKACLWDDSEYLNFDDVASLAIGYKIITKY"
     gene            complement(2567..3379)
                     /locus_tag="PK348_01640"
     CDS             complement(2567..3379)
                     /locus_tag="PK348_01640"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HXK64945.1"
                     /translation="MKVFSKATIPCACYILIVTICILFQGCYLGLEYSPYSTDVDYKN
                     LNKKNIDRIKARESESSNYRIALIADTHRFYTETENVIKVLNSRNDLDFVFILGDITD
                     VGLLKEYNFAYDIFSKLYCPFIVIAGNHEFLGYGENIYKKMFGPLNFNFTFRGTEFIC
                     FNDSNWESGEPDLWWLEQTAALSYEQHKIVLAHIDCSITSERFNQEQVDRFNDIVKSY
                     FDMAFHAHDHVNSYKLIEDIPRYHVGSPQHRHYVILEYDYNTDNFTVELCTF"
     gene            complement(3422..3709)
                     /locus_tag="PK348_01645"
     CDS             complement(3422..3709)
                     /locus_tag="PK348_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK64946.1"
                     /translation="MVMLFIVYSNAVDDEVVEIVKKYASGYTKFVDVQGEGNKEPQLG
                     THIWPGLNNCMMIALEDNKSKDKIAQELQKLKVKFKGVGISVFVVQLKEIL"
     gene            complement(3740..>6558)
                     /locus_tag="PK348_01650"
     CDS             complement(3740..>6558)
                     /locus_tag="PK348_01650"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HXK64947.1"
                     /translation="MGLAVSDMRERIDIRKRFLPDDVKNPVVLKFDISMMPVVMLSVT
                     GPKDLAYVKKYAEDNLKNLFEQTDGVAAAYVSGGQSYEVKVELIKNRIDAYKLTIDQI
                     IQLLAAENLNIAGGDVKTPYMKYTLRTQGEFKSLDDIRNVVVTVKNGTPIYVRDIARV
                     YLAPADVKEIVRLNGENGVILRVQKQSDKNTVIVARNILKKAEEVRKTLPKGMDIQPF
                     FNAAEFIELSISNMIKNAVEGALIAILVVLVILRNMRAALIMGLSIPVSIITSFVLMY
                     FFDLSLNMMSMGGLAIGVGMLIDNSIVVLENIFRYREKGARPNEASKLGADEMGMAIT
                     ASTLTNLVVFVPFLFSQGLAVQLFKDMALTITFSMLSSLVVALSLVPMLAAKFIPRIE
                     HTYTGKLSFLQPLFDRSEQYFIRLERFYGKVINWALDHRRRVIRYVAISFVIGLIMIP
                     IAGMEFMPEYDDAQLTIVAKLPVGTNLETTESVMKMIEKRALDVLKKEEYVVVSVRAG
                     YGEGLTAAFGETTDYTGKIEMRLVQPSKRKRSKNEIRQALREAFKGIPGVTFNFSLQG
                     NAGGRILGMGGATISIEVYGYDFEKSSQYVEDIRNAIKNIPGLKDIDVSREEGLPEKV
                     IVVNREKASKMGVNAYQIANLIKNNVAGYPATRYRFEGDEYDINVRLREADRETIEHI
                     KSIEINTPIGKRVPVGNLIEIVTKSGPSTIERKKQERVVYLNCKAEGRALSAVVWDIN
                     KAISKLPKPANFDVVIAGAYKDMQDTFQDLLLVMLVAMVLTYVIMAAQFESLLRPFIV
                     MSSVPTVIFGVSLFLFLTGTTFNVVTFLGVIMLVGIVVNNAIVLVDYTDILRKRGYGI
                     REALVEAGTKRLRPIMMTTLTTILSLIPMASGLGEGSELSSPLARAVMGGMSTAFIFT
                     LVFIPVMYMIFETIRERYEARRKY"
BASE COUNT         2058 a         1340 c         1040 g         2120 t
ORIGIN      
        1 attccatacc atgctatatt ccgttgtttg tatgtacaac gatatactgg atgacagcgc
       61 tattgcccgc acactgttag ctgccacaca ggacaccttt tattatcaca tgatgtactt
      121 ccaggacacg tatgacagat taccagtgcg gatatttcag ttatttgaaa cgctggaaac
      181 atttctacac gcacaggaac actatgcgtg tattaatccc atactgctag taacacctga
      241 taattataca agaatttcac aaaaagattt tgcctatccc ttccccattg ttatacagaa
      301 aggttttgca cgccactgca taaactggct taagcaacag tacagtgaaa gcatagagct
      361 ggtaagcttt caggatgaag ccgaccagaa atatgtatat aatgaacttg gtatagaata
      421 tgtaaaaatt gtttttaata cctactttcc ataaattctt aactttcatt aataattata
      481 atttcaatat aaattggtaa tatttcacaa aacttaatcg tgattgtttg taagcgtcaa
      541 attaaccata ggggtattga tatgcgatga aattttaaaa agcaaggaaa gtttattttt
      601 tttattctat tgtttcagat aagcaatata gtattaaacc ataacctgtg tcaccacttt
      661 acggtaaagt cagcaatgaa atataattgt aatttacgaa accaccatgt aaattgatta
      721 ttatcaaaat tatgttccct ccactttgct gctttatttt ggccccttat tccatactgc
      781 cgtatgtgct cttcacacta ctgccgcttt gtgtttctgg taaatgtgct tgtttcatta
      841 ctgtttgcca ccttatggac aatgttttat ttttatgagg gtggtagtta tgtgagtggg
      901 taatttatat ggtttctaaa attatatgta tatacaaaaa catatttcat cgttccgtgt
      961 atctattaat catgtattgc tacattttca tttctacgct taaattatga ttgcaaattg
     1021 tcttagcata tcatagtaac gatgatacac atatacttct ggagggaaat catgaatata
     1081 gaaacaacca cctgcattgc gtatgaaaat gtaaaacttt tgcaggatta tgctgatgtc
     1141 tacaaactat cgcttcatac gtttattatt aattttatta cctatgtgat gtcatacaaa
     1201 aagattccgg taaaaagctg caggcggctt acgtatcgca agcgctatga acgctggaag
     1261 cgggtgcatc tgtatctgta cgagaatgag tatgagtttt taatggatgt gaggaaagtg
     1321 tgcaagatgt ccattgctaa ggttattgcc tactgtattg aaaactatct ttttgatttt
     1381 cttgcagcgc ttgatagtga agataatacc gataactatc gatttagcga atacacattt
     1441 atgttttatt tagaaaatgg catacaatgt tgtcgatttt actggggacc acacccggaa
     1501 ttggtgaaac tggccatgca gtaaccattt acaacaccaa tttcttccca ctttataggg
     1561 atagtgcaaa tccttgagga tataactatt attttttacc tgaacgatac tgcatttctt
     1621 aatccgcttt cctcatactg ctacaggtgc tctttcctat actcacgctt tgtgcttctt
     1681 tgcacatgtg ctttttccat tgtaatattc ctccttctgt acaatattat tttacatagg
     1741 aaagatatac actatgtttt atttttgtaa agatggtgtt aatttgacgg gtacgtcttt
     1801 gtgtccatac aatgtatttt taaaatcaac aggaatagta ataatgatgg ataataactt
     1861 ttatgtaagg ttgtttcact attgctatta cacttataat ctacagtata aactaacttt
     1921 ttttatagca attatctctt tttattcatc aaattttttt aatactttgt tattatttta
     1981 tatcctatag caaggctagc aacatcatca aaatttagat attcactatc atcccagagg
     2041 catgctttta aatacgtatc aagtatggtt atttcaacat aaaaaccatg tattaaacct
     2101 atcattgtgc ttttcccctg tatctgtaat ccaacactta cggcactatg ccttcccgtg
     2161 gggttataat acctgtcagg gcagtttgtg gatctttccc aataaacatt tttatccatc
     2221 acaaaaagca tcgtgacacc aaaataaaat ggcaatagta cattatcatc taaaaaattt
     2281 aaagtaaatg ggtatacgat atttttccag gaaactgtat gtatctcctt acctgtaaca
     2341 tctgcaggac taaaaccata caatagagag ctttcccagg tattgttgaa agaaagaaaa
     2401 ccaatgccca tagctataaa tccaatatac ccggcagttt gtatggtata acaatcagga
     2461 taataccata attcattttt gccatgccca tgtgaaggtt catcacaagc aaaaataaaa
     2521 aatatggtta taattaaatt agtaagtagc ttatagaaat tttctcttaa aatgtacata
     2581 attcaacagt aaaattatct gtattgtaat catattctaa tatgacataa tgtctatgtt
     2641 gtgggctacc cacatgatag cggggaatat cttcaatcag tttataactg ttgacatggt
     2701 catgagcatg aaaggccata tcaaaataac ttttaacaat gtcattgaat ctatctacct
     2761 gttcctgatt gaatctctca gatgtaattg aacaatcaat atgagccaat actattttat
     2821 gttgctcata acttaaagca gcagtctgtt ccaaccacca caaatcaggt tcaccgcttt
     2881 cccaattact atcattaaag caaataaatt cagttccacg aaacgtaaaa ttaaaattta
     2941 atggcccaaa catcttttta taaatatttt cgccatatcc aagaaattca tggttgccag
     3001 caatgacaat aaaagggcaa tacaattttg aaaatatgtc ataggcaaaa ttatattctt
     3061 tgagtaaacc aacatcagta atatcaccta aaatgaatac aaaatccaga tcattccggc
     3121 tatttaatac tttaataaca ttttcagttt ctgtgtagaa acgatgggta tcggctataa
     3181 gcgcaattct ataattactg gattctgatt cacgcgcttt aatacggtca atatttttct
     3241 tattgagatt tttataatct acatcagttg aatacgggga atattccagc ccaagataac
     3301 acccctgaaa tagtatacat atagttacta tcaggatata acaagcacag ggtatagtag
     3361 ctttactgaa aactttcact gctttaaacc tctgttttgc tgtgtcagta atccttattt
     3421 tctatagaat ctctttcaac tgcaccacaa agacactgat gccaacacct ttaaatttta
     3481 cttttagttt ttgtagctcc tgggcaattt tatccttaga tttattgtct tcaagagcta
     3541 tcatcataca gttattgagc cctggccata tatgggtacc caattgcggt tctttatttc
     3601 cttcaccctg cacatctaca aattttgtat atcctgaagc gtattttttt actatctcaa
     3661 caacttcatc atcaacagcg ttgctgtaaa ctataaatag cattaccata ctaattatct
     3721 cctctgcaaa taaatttaat taatatttcc gtcgtgcttc ataccgttca cgtatggttt
     3781 caaatatcat atacattacc ggtataaaca ccagggtaaa gataaacgct gtactcattc
     3841 cacccataac agcacgcgct aatggagatg aaagctctga tccttcgccc aggcctgaag
     3901 ccataggtat aagcgatagt atcgtagtta gcgtagtcat cataattggt cgtaatcgtt
     3961 ttgtacctgc ttcaaccagt gcctctcgta tgccataccc gcgctttcgc aggatatcag
     4021 tataatccac aagaactatc gcattgttaa caacaatacc aactagcatg atgacaccta
     4081 aaaatgtgac cacattgaat gttgtaccgg ttaagaacaa aaatagtgac acaccaaaaa
     4141 taacggtagg cacactggac ataacaataa atggcctaag taacgattca aactgagctg
     4201 ccataataac ataggtcagc accatggcaa ccagcattac cagcaataaa tcctgaaatg
     4261 tgtcctgcat atccttatat gcaccagcta tcaccacatc aaaattagca ggtttgggca
     4321 atttactgat tgctttgtta atatcccaaa cgacagcact caaagctcta ccttcagctt
     4381 tacagtttaa atatacaaca cgctcttgct ttttccgctc aatcgttgac ggccctgatt
     4441 tagtcacaat ttcaatcagg tttcctactg gcactcgctt gccaatggga gtgtttatct
     4501 caattgattt tatatgctca atagtttctc tgtcagcctc tcgtaaacga acattaatat
     4561 catactcatc gccctcaaag cgatatcggg ttgccggata ccctgcaaca ttatttttta
     4621 taagatttgc aatctgatag gcattgacac ccatcttgga agctttttca cggttaacaa
     4681 ctataacctt ttcgggcaat ccttcttccc gggaaacatc aatatctttt aaacccggaa
     4741 tatttttaat agcattgcgt atatcttcaa catactgtga tgatttctca aaatcatatc
     4801 catatacttc aattgaaatt gtagctccac ccattcctaa gattctacca ccagcattac
     4861 cctgaagtga aaagttaaac gtaacacccg gaattccctt aaatgcttca cgtaaagcct
     4921 gccgtatttc attttttgac cgtttgcgtt tacttggttg aaccagcctc atttcaatct
     4981 taccagtata atctgttgtt tcgccaaacg ctgcagttaa cccttcccca taaccggctc
     5041 tgaccgaaac aacgacatat tcttcttttt ttaacacatc aagagcacgt ttttcaatca
     5101 tcttcataac ggactcagtt gtttccaaat tagttccaac gggcaatttt gcaacaatag
     5161 tgagttgagc atcatcatat tccggcataa attccatccc ggcaataggg atcatgataa
     5221 gcccaatcac gaatgaaatt gcaacgtatc ggataactcg ccttctgtgg tcaagtgccc
     5281 agttgataac ttttccataa aaccgttcca atcgtataaa gtattgttca cttctgtcaa
     5341 ataatggctg taaaaatgaa agtttcccgg tataggtatg ttcaatgcgg ggaataaact
     5401 ttgcagcaag cattggcaca agtgatagtg ccacaaccag tgaacttaac attgaaaacg
     5461 ttatggtaag cgccatgtct ttaaatagct gtacagcaag cccctggctg aataaaaatg
     5521 gtacaaagac aaccaggttg gtaagggttg acgctgtaat ggccataccc atttcatcgg
     5581 cacctaattt tgacgcttca tttggccgtg cacccttctc cctatatctg aaaatatttt
     5641 ccagtaccac aatggagtta tcaataagca taccaacacc aatggcaagc ccacccattg
     5701 acatcatgtt gagtgacagg tcaaagaagt acatcagaac aaatgaagta ataatagaaa
     5761 caggtatcga caatcccata atgagtgcag cccgcatatt tcgcagtata acaaggacaa
     5821 caagtatagc tattaaagcc ccttcaacag cattttttat catattggat attgatagct
     5881 ctataaattc agcagcattg aagaatggct gtatatccat ccccttgggc agggttttgc
     5941 gtacttcttc agcttttttt aaaatattgc gggcaacaat aacggtattt ttatccgatt
     6001 gtttctgaac acgcaagata acaccatttt caccattaag gcgtactatc tctttaacat
     6061 cagcaggggc taaatatact ctggctatat ctcgcacata aattggggtg ccatttttta
     6121 ctgtaacaac cacattgcga atatcatcaa gagacttgaa ttcgccctga gttcgcaagg
     6181 tatatttcat gtatggggtc ttgacatcac cgccagcaat atttaagttt tctgccgcca
     6241 gaagctgtat aatctggtcg atagtcaatt tataagcatc aatcctgttt ttaataagct
     6301 caaccttaac ttcataactc tgcccaccac tgacgtatgc cgctgctaca ccatcggtct
     6361 gctcaaataa attttttaaa ttgtcctcgg catatttttt tacatacgct aaatctttag
     6421 gtccggtaac tgaaagcatg acaacaggca tcatggaaat atcaaattta agtaccacag
     6481 gatttttaac atcatcaggt aaaaagcgtt tgcggatatc aatacgctcg cgcatatcac
     6541 ttactgccag ccccatgt
//
LOCUS       DAOSZP010000040         6475 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_10131_length_6475_cov_2.823520, whole genome shotgun sequence.
ACCESSION   DAOSZP010000040 DAOSZP010000000
VERSION     DAOSZP010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6475)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6475)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6475
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10131_length_6475_cov_2.823520"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            208..1887
                     /locus_tag="PK348_01655"
     CDS             208..1887
                     /locus_tag="PK348_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006967284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HXK64948.1"
                     /translation="MEKVWLKSYAPGVPHSIKYDEITVVHGFERSVKEYPNKDALIFL
                     DAKISFKKMQSMINKIANALLDMGVKPGDKVAMLMPNMPQIVIGSYAAWKIGAVVVMN
                     NPLYTDHELEYQLNNSESTVLITLDLLGPRMIALRPKTPVKKIIIAHIRDHLGFPKKQ
                     LLPIVAKDKHKDIPPTQDVYEWTDLLKKYPDKDPGIMAKFNDLANLQYTGGTTGVSKG
                     VMLTHKNLSCNLQQISAWFPGFKRGELTSLGILPYFHSFGLTTVMNLCLWQGWTQVLI
                     PRPEPGAILEAIVKHKVNFFPAVPTMYVGLLNHPKIKDTDISSIKGCFSGAAPLPVDV
                     IKEFEAKTGAQICEGYGLSETSPVATINPYGGKTIQGSIGLPVPDTELKIVDLEEGTK
                     EMPLGQEGEVCISGPQVMSGYYKMPDETAKTLRDGWLYTGDIGKMDENGYFYIVDRKK
                     DMIIAGGYNIYPRDIDEVLFEHPKIMEACAIGVPDKYRGETVKAFVVLKPGETMTEQE
                     VIDYCKTKLAAYKVPKMVEFIDSLPKSNVGKILRKEIRKMELEKMQKEQAG"
     gene            1962..2642
                     /locus_tag="PK348_01660"
     CDS             1962..2642
                     /locus_tag="PK348_01660"
                     /inference="COORDINATES: protein motif:HMM:NF016163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF429 domain-containing protein"
                     /protein_id="HXK64949.1"
                     /translation="MYYIGIDPSWSGKNYTAVVMCDDALQVIDTCYTNDIKKIISAIV
                     PFNDAIIGIDAPLIIKNATGHRRHEIEFLRVFSRFGLGLHAVNKKRYPYFFPEVLYKE
                     LHALGFSFNNNNIFEVYPHATIMTCFNNMKLLQYKSKYGVTMRRENIEKLYNYLAAVI
                     KCSDLFTGTIAQAKGKQLKKYEDYLDALVCAYTVYYCSRKNCYSFGDAENGILLVPSP
                     ECRKVVDT"
     gene            2735..4069
                     /locus_tag="PK348_01665"
     CDS             2735..4069
                     /locus_tag="PK348_01665"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXK64950.1"
                     /translation="MAEQKFAKHEIFAILLIALMNLFLFADQNLMAPNLTQIAHDFGF
                     NDVQRDVMLGGRISFVFWVLGGLVTLAIGYLTDKISRRDLFLFVILVGEIPCLLTGFA
                     QNYNQLFWLRALTGIGIGGALPLTFSLIGDYFSHKNRAAAAAWIGLAQGLGIAVGQLM
                     AGFIGPVYGWRLPFILVAIPNFLLAIIFLLFVKEPQRGKTEESLKELIEEGIAYTGRI
                     NWKEYKNLFKIKTNILVFLQGIPGTVPWGVFFIFLNDFYSQDKGYSVQMATLIVMTVG
                     AAAIIGAFVGGLIGNKLYNIKPKYLPMLCGTSTLIGIIPMALLLNYNPQIIVPEPDPT
                     WPLIFGFVTGFTVTITGPNVRAILLNVNTPETRGSIFSLYNLTDDLGKGFGPVIISAL
                     IVWFGRIMAFNVANLFWLVCGLLLLVMIWTFPEDEAKLNAILKERAAQMKKI"
     gene            4096..>6475
                     /locus_tag="PK348_01670"
     CDS             4096..>6475
                     /locus_tag="PK348_01670"
                     /inference="COORDINATES: protein motif:HMM:NF015644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HXK64951.1"
                     /translation="MNKKFLIALIVFMVIYILVYSVGNFYIDYRWFSIHHHQAIFWIL
                     FFSKFNVQTLFWGLFIGLFILNFILIRVLGGKGRIFTKNILDRIRIPGFGTTRNLLFV
                     LLAAGVIVLGFFMGLWASAYWKEYLMYKNAVPFTGFPNDPLFNKDIGFYVFTLPFYKF
                     MFRWILVSLLIITVFSFFFHILNNGIFSQGGKLEFSRFARAHLSTLMGLIVLLIATGY
                     RLLSYDLLYNNRAKFFGAGYTDVHAKLLAYDICIVISVIAAILLFANIITRSFKLPVI
                     ILITLLPVYFIFGTIFPALQQRFIVDPNELEKESPYIKYNIEFTRLAFGLNTIKEMPF
                     ENAATLTLRDLEKSKDVVNNIRLWDWRPLKQTYKQLQGLKPYYDFIDVDIVRYTVNNQ
                     KVALNISARELDNSKLTVSKNSWLNNHLIFTHGYGIVASRVDRITPEGLPELVVYDIP
                     PKYRIPIPVEVPQIYYGEHDNSYIITNTRVTPGEFDYPYGDENKYTIYNGTGGDVLDS
                     VIKRAMYAIAFGDFNILISNVITNTSRIHFRRNIKEMINAITPFLYYDSDPYVVVAGG
                     KIYWIIDGYTSSNAFPYSTPVQLEYGESINYIRNSVKIVIDAYNGSITYYISDPDDPV
                     INAYSKIFKNLFLPIDKMPSELQVHLRYPEGLFNLQAQQLLRYHMTNINVFYNNEDLW
                     EIPLQIYENEKEYMHSYYLVTALPGEKATEFLLILPFTPAKKDNMIAFMVARCDMPYY
                     GQLLLYTLPKDKLNFGPMQIEARINQDAEISKQLTLWSQRGSRVIRGNMLVLP"
BASE COUNT         2086 a          998 c         1287 g         2104 t
ORIGIN      
        1 taaaaattat aattaatata caaataatta taaatataaa agtatattat gttatcatta
       61 ttcaataaaa ttataaatat gttatatata aataaatagt tgacattgtt atcatatatt
      121 gtttgaataa agtcataaaa tacaaattca cttcaataat ctatcgtttc tgttttttta
      181 aattaaaaaa atcgcaggag gtttgcaatg gaaaaagtct ggttaaaatc ctatgcacca
      241 ggtgtacctc attcaatcaa atatgatgag ataaccgttg tgcacggatt tgaaaggtca
      301 gtaaaagaat atcccaataa ggatgcgctg atctttcttg atgctaagat cagttttaag
      361 aaaatgcagt caatgattaa caaaattgca aatgcattac ttgatatggg ggtaaagccg
      421 ggggataagg ttgcaatgtt aatgccaaat atgccacaga tagtcattgg atcgtatgca
      481 gcatggaaaa ttggtgctgt tgtggtaatg aataatcctt tatatactga tcatgaactg
      541 gaatatcagc taaataattc tgaatcaact gttttgataa cactggattt gttaggtcca
      601 cgcatgattg cattacgccc aaaaacaccg gttaaaaaaa ttatcattgc tcatatccgt
      661 gaccatttag gatttcccaa aaaacaactt ttgccaatcg tggcaaaaga taaacacaaa
      721 gacatcccac caactcagga tgtatatgaa tggaccgatt tattaaagaa ataccctgat
      781 aaagatccgg gtataatggc aaaattcaat gatctggcca atttacagta tacaggtgga
      841 acgactggtg taagtaaagg tgtaatgctt acgcataaaa atttatcatg caatttacaa
      901 caaataagtg catggtttcc gggatttaag cgcggtgagt taacaagcct gggaatttta
      961 ccgtatttcc attcatttgg attgaccacg gtaatgaacc tttgcctctg gcagggttgg
     1021 acacaggtgt tgataccacg accagaacct ggtgccatcc ttgaagctat agtaaaacat
     1081 aaagtgaatt tcttcccggc tgttcccacg atgtatgtag gattgttgaa tcatccaaaa
     1141 atcaaagaca ccgatatatc aagtattaag ggatgttttt caggtgcagc gccattgcct
     1201 gttgatgtta ttaaagagtt tgaagcaaaa acgggtgctc aaatttgcga gggttatgga
     1261 ttgtctgaaa cttcgcctgt tgcaacaata aatccgtatg gtggaaaaac aatacagggt
     1321 tcaattggat tgccagttcc tgatacagag ctaaagattg ttgatctgga agaaggtaca
     1381 aaagaaatgc cattggggca agaaggtgaa gtatgtatca gtggacctca ggttatgagt
     1441 ggttattaca agatgcctga tgaaacagca aagacacttc gcgatggatg gttatataca
     1501 ggtgatattg gtaaaatgga tgaaaatggc tatttctata ttgttgatcg gaagaaggat
     1561 atgattattg caggtgggta taacatctat ccacgtgata tagatgaagt gctctttgaa
     1621 catccaaaaa ttatggaagc atgtgcaata ggagtgcctg ataaatatcg tggtgaaacc
     1681 gttaaagcgt ttgtagtatt gaagcctggt gaaaccatga ctgagcagga agttatagac
     1741 tattgcaaaa caaaattagc tgcatacaaa gttccaaaga tggtagaatt tatcgacagt
     1801 ttgccaaaga gtaatgttgg gaagatattg cgtaaagaaa tcaggaagat ggaacttgaa
     1861 aagatgcaga aagagcaggc tggataaaat ataaagcatt tataaaaaca tttgataagg
     1921 cgcacaccta gtgcgtctta tctttttatc aggattatag tatgtattat attggcatag
     1981 acccctcatg gagtggtaaa aattatacag cagtagtaat gtgtgatgat gcattgcagg
     2041 ttatagatac ttgttataca aatgatatta aaaaaataat ttcggcgata gttcctttta
     2101 atgacgctat tataggtatt gatgcacctt tgattataaa aaacgcaaca ggacatcgca
     2161 ggcatgaaat tgaattttta agagtatttt cgcgatttgg gcttggactg catgcagtga
     2221 ataaaaaacg gtatccatac tttttccctg aagtgttata caaagaattg catgctctcg
     2281 gtttcagttt taataacaac aatatttttg aagtatatcc tcatgcaaca attatgacat
     2341 gttttaacaa catgaaactt ttacagtata aatctaaata cggtgttact atgcgcagag
     2401 agaatattga aaaattatat aattatttag cagctgtaat taagtgtagt gatttattta
     2461 cgggaactat cgcacaggct aaagggaaac aattaaaaaa gtatgaagat tatcttgatg
     2521 cattagtgtg cgcatatacg gtatattatt gttcgcgtaa aaattgttat tcatttggcg
     2581 atgctgaaaa cggtatttta ctggtgcctt ctcctgaatg cagaaaggtt gttgatacat
     2641 aatttaacaa aaaatccttg aatttattat ggattaatgg tatttacaat gcactttcgt
     2701 atttcaaaag tatatacatc gtgggggaat aagtatggct gaacaaaaat ttgctaaaca
     2761 tgaaatattt gcaatactcc tgattgcatt gatgaattta tttttatttg ctgatcagaa
     2821 tctgatggca cccaatctga cacagattgc ccatgatttt ggttttaatg atgttcagcg
     2881 agatgttatg ttaggcggac gaatatcgtt tgtattctgg gtgctgggcg ggcttgtgac
     2941 tcttgccatt ggttatctga ctgataaaat atcacgtcgg gatctatttt tatttgttat
     3001 tcttgttggt gaaattccgt gtctgcttac tggttttgca cagaattata atcagttatt
     3061 ctggctcaga gcattaacgg gaattggaat tggcggagca cttccattaa cattttcact
     3121 gattggggat tatttttcac ataaaaacag ggcggctgct gcagcatgga ttgggcttgc
     3181 acagggttta ggaattgcag tggggcagct catggcaggg ttcattggcc ctgtctatgg
     3241 atggcggtta ccatttatac tggttgcaat acccaatttt ttactggcaa ttattttctt
     3301 gctgtttgta aaagaaccac aacgtggaaa aacagaagaa agtcttaagg aattgattga
     3361 agaaggtatt gcatatactg gccgtatcaa ctggaaagaa tataaaaatt tatttaagat
     3421 taaaaccaat atccttgtct ttttacaggg gataccgggc actgttccat ggggtgtatt
     3481 ctttatattt ttaaatgatt tttattcaca ggataaagga tattcagttc agatggctac
     3541 actcattgtt atgacggttg gtgctgctgc aataattggt gcatttgttg gtggacttat
     3601 tgggaacaaa ctgtataata ttaaaccaaa atatttgcca atgctttgtg gcacatcaac
     3661 gcttatagga ataataccaa tggcattgtt gcttaattat aatccacaaa taatagttcc
     3721 cgaacctgat ccaacctggc cacttatttt tggatttgta actggattca cagtaactat
     3781 taccgggcct aatgtaagag caatattact caatgttaat actcctgaaa cgcgtggttc
     3841 aatattctca ctttacaatc tcactgatga tcttggtaaa ggatttggac cggttatcat
     3901 cagtgcactg attgtttggt ttggaaggat tatggcattt aatgttgcaa atcttttctg
     3961 gcttgtgtgt ggattactgt tactggttat gatatggaca ttcccggaag atgaagctaa
     4021 acttaatgca atattaaaag aacgcgctgc acagatgaaa aaaatttaat gataaagaaa
     4081 ataatggagt aagccatgaa taaaaagttt ctaatagcac tgattgtttt catggtcata
     4141 tatatactgg tatattcagt gggtaacttt tatattgatt atcgctggtt ctcaatccat
     4201 caccatcagg ctatattctg gattcttttc ttttcaaagt ttaacgtgca gacgttattc
     4261 tggggtttgt tcataggatt attcatactt aattttattc ttataagggt tctgggcggc
     4321 aaaggtagaa ttttcacaaa aaacatactg gacagaatac gtattccggg atttggtaca
     4381 acgcgcaacc tgttgtttgt cttattggct gcaggtgtaa tagttttagg ttttttcatg
     4441 ggtttatggg cttcggctta ctggaaggaa tatttaatgt ataaaaatgc agttccattt
     4501 actggcttcc caaacgaccc cctgtttaat aaagacattg gattttatgt attcactctc
     4561 ccgttctata aattcatgtt ccggtggata ctggtttcat tattaattat aactgtattc
     4621 tcttttttct ttcatatatt aaataatggc attttttcgc agggtggcaa gctggagttt
     4681 tctcgttttg cacgggctca tctgtcaaca cttatgggtt taatagtatt gctgatagca
     4741 accgggtata ggttactgtc atatgattta ttatataata acagggcaaa gttttttggt
     4801 gcaggttata ccgatgttca tgccaagcta cttgcttatg atatatgcat agtaatatca
     4861 gtaattgctg ctattttact ttttgctaat ataattaccc gcagctttaa attaccagtt
     4921 attattttaa taacattatt gcctgtttat tttatatttg gcactatctt tcctgcgttg
     4981 cagcagcgtt ttattgttga cccaaatgag ttggaaaaag aatcaccata tattaaatat
     5041 aatatcgaat tcactcgatt ggcttttggg ctcaatacaa taaaagagat gccatttgaa
     5101 aatgcagcta cgttaacatt gcgggattta gaaaagagca aggatgtggt caataacata
     5161 cggctctggg attggcgtcc tttaaagcaa acatataaac aattacaggg attaaagcca
     5221 tactatgatt ttattgatgt agatatagta cggtatactg taaacaatca gaaagttgca
     5281 cttaatattt ctgcacggga attggataac agtaaattaa ctgtttcaaa aaattcatgg
     5341 ttaaacaatc atttgatttt tactcatggg tatggtattg tagccagtag agtggacaga
     5401 attacgcctg agggtttgcc agaacttgta gtgtatgata taccgccaaa atacaggata
     5461 ccgatacctg ttgaagtacc acaaatatat tatggagagc atgataattc gtacattatt
     5521 accaatacaa gagtgactcc tggtgagttt gactatccgt atggtgatga aaataaatat
     5581 acaatatata atggaacagg cggtgatgtg cttgattcgg ttattaaacg ggctatgtat
     5641 gcaatagctt ttggtgactt taatatctta atttctaatg taataaccaa taccagcaga
     5701 atacattttc gtagaaatat taaagagatg ataaatgcca tcactccatt cttatattat
     5761 gattctgacc catacgttgt agttgcagga ggtaaaatat actggattat tgatggatac
     5821 acatcttcaa atgcatttcc gtattcaaca ccagtacaac tggaatatgg tgaatcaata
     5881 aattacatac gtaattcagt aaaaattgtc attgatgcat ataacggtag cattacatat
     5941 tatatctctg atccagatga tcctgtgatt aatgcatatt ccaaaatttt taaaaattta
     6001 tttcttccca ttgataaaat gccttcagaa ttgcaggtac acctgcgtta tcctgaaggt
     6061 ttattcaatc ttcaagcaca gcaactgttg cgatatcaca tgacaaatat aaatgttttt
     6121 tataataatg aggacctgtg ggaaattcca ttgcaaatat atgaaaatga aaaagaatat
     6181 atgcatagtt actatctggt aactgcttta ccaggagaaa aagcaacaga atttctttta
     6241 atattgccgt ttacacctgc caagaaagac aatatgattg catttatggt tgcccgttgt
     6301 gatatgcctt attatggcca actactactg tacacactcc caaaggacaa acttaacttt
     6361 gggcctatgc agattgaagc acgaataaat caggatgcag aaatttctaa acagctaaca
     6421 ttgtggagtc aacgtggttc acgggtaatt cgtggcaata tgctggtact gcctg
//
LOCUS       DAOSZP010000041         6451 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_10178_length_6451_cov_3.742652, whole genome shotgun sequence.
ACCESSION   DAOSZP010000041 DAOSZP010000000
VERSION     DAOSZP010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6451
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10178_length_6451_cov_3.742652"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(96..938)
                     /locus_tag="PK348_01675"
     CDS             complement(96..938)
                     /locus_tag="PK348_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012505031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HXK64952.1"
                     /translation="MNKIIQNSKISENVGKAIIEAPYIAHHRKAGQFVIVIVDEVGER
                     IPLTIADSDREKGTITLYYQIVGTSTHKLSLKKEGEYVYDVAGPLGHPTDIRNYGTAV
                     CVGGGIGIAAVYPIAVAMKEAGNFCINIIGARNKDLLILEQELKAKSDQIIVTTDDGS
                     YGIKGFVTDELKKILDSKKVDIVVAVGPVPMMKAVSELTRRYNVKTLVSLNSIMVDGT
                     GMCGGCRVTVDGKKQFTCVDGPEFDAHLVDFDELMKRLNTYKDQEKESFEKHTCQLEK
                     YHGR"
     gene            1303..2901
                     /locus_tag="PK348_01680"
     CDS             1303..2901
                     /locus_tag="PK348_01680"
                     /EC_number="1.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013257519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HXK64953.1"
                     /translation="MGNLSRPNSNDATRTANRSRSVAPQSGICSRCLDGCTGNCEIFQ
                     ATMRGRELLYPGPFGEITAGADKDYPVDYSHLNIQGYAAGAKGLLNPGPETARFPLVS
                     TETYFGTKNRVKLEVPMFTGALGSTDIARINWEHFAVGAAISGIAIVCGENVCGIDPG
                     LERDKNGKVTKSPEMIRRVETFKKYKGEAGDILVQMNVEDTNLGVAEYIISQLGVETV
                     ELKWGQGAKCIGGEIKVRSLERAIELKNRGYIVTPDPTIPAIQAAFKDGAIKEFERHS
                     RLGFISKEAFLQEVERLRALGAKRVTLKTGAYSQKELAAALRWASEAKIDLLTIDGAG
                     GGTGMSPWRMMEEWGVPTFYLQCMAYDMAQKMASKGMWVPDLAMAGGFSTEDHIFKVL
                     AMGSPYFKAVCMGRALMIPGMVGKNIEKWLKENNLPKNVAQHGATKEEIFVCYEELKA
                     KYGKEVDNFPLGAIGLYTFSQKIKVGLQQIMAGSRNFRIDTISRSDLMALTEEAAKIS
                     GIPYVMDAYKEEAEKILLEGCDYI"
     gene            complement(3077..4987)
                     /locus_tag="PK348_01685"
     CDS             complement(3077..4987)
                     /locus_tag="PK348_01685"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HXK64954.1"
                     /translation="MGELKTMVQYFYDTCSKFPERPAQKFNSKLYDDNNGMFTYAELK
                     ERVEDIACALLKIGFLHKGRAAIMAPTSYMWTQVDIAIACAGGVSVTIYPTLSDTEVT
                     YIMKDSKSTVIFVGNKEELDQIKRIWKQLKDLKTVIVMDTSFKGDNKKVYGIKDFIAI
                     GREWRKNAANYKKYIKRKDGVKLDDWFTILYTSGTTGQGKGAIITHWTGSSRLEGTVA
                     YFQKYNFDVNEHDVTLCYLPLSHVFDRGSCQWLALTKGSLICYADKPATLIEDMQKYN
                     PTWINCVPRLYEKIYITMQQAMAESTLKKKLFDWAIGVGLQALEYRKDANGCYNMHPE
                     FDLKSKLPLGLKIKYSIADKLFAKVRGLFGNRFHHSFSASAAISPDLLRFYYALGLAV
                     VEGYGSTESFNAAVLNPITACKPGCMGKEANGSKCRVAADGELELSGAGIFKGYLNKP
                     KETKEAFTKDGWFKTGDVVELTPDGYIKFVERKKGIICTSIGKNVAPAKIENLFSTSS
                     WVEQIFLVGDEKPYISALIVPNFANFIALFKREGIAFDESKLVYENIGGMQICTKVGD
                     DFINNKRLQELIDNEVKEANKRLEKFEEVKRYTILNQRFTESNGMITPTQKAKKRVII
                     QVYKDVIEKMYE"
     gene            complement(5082..>6451)
                     /locus_tag="PK348_01690"
     CDS             complement(5082..>6451)
                     /locus_tag="PK348_01690"
                     /inference="COORDINATES: protein motif:HMM:NF014342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HXK64955.1"
                     /translation="KMEEQRNRGRQSWKGVEGRIDGVVGEILKKVQSTQFEGYEKYDS
                     ESEIIVLSDFESLKTHLNEGQEGILVVKNTPFYGESGGQVGDTGYIIGDSGTFRVVDT
                     KKSGDLIVHIGQVEKGTLQEGTKVRLSIDGLRRNLTRANHTATHLLQAALRHVLGQHV
                     KQAGSLVDPQHFRFDFSHFKAMTQEEIQQVEAIVNEKIWAALPVNTAIIPLQEALNQG
                     AMAVFDEKYSDIVRMVSISDFSKELCGGTHVDNTGKIGVFRIMRESSPGAGVRRIEGV
                     TLKGVYERLIHEHAIVRRIMGLLEVNESNIEKKVAEMLEKVSYLEKELEKAKAAGLKD
                     IARELYTKAEHINSLKFVSASLQNMDAEALRTLADYIRELSDTLVVCLASSKENKAFI
                     IAAATKGAIDKGIDCGAIIKTVSKEIGGGGGGRKDLAQAGGKSPEHIAKALAKCREIV
                     LHSSK"
BASE COUNT         1868 a         1425 c         1101 g         2057 t
ORIGIN      
        1 gcactgctgt tttttcatca taccccaaag ggacttcgtt aaagttatgg atccgtttgg
       61 ctggatcctg ctgaggcatc ttttgtcttg ggattttatc ggccatggta tttctccagc
      121 tgacatgtat gtttttcaaa gctttctttt tcctgatcct tataggtgtt gagccgtttc
      181 atgagttcat caaaatcaac taaatgagca tcaaactcag gtccatcaac acaggtaaat
      241 tgctttttgc catcaactgt tactctgcac ccaccacaca tgccggtgcc atcaaccata
      301 atagaattca ggctcaccag cgtttttaca ttatacctgc gtgtgagttc actgaccgct
      361 ttcatcatgg gtacaggccc cactgcaacc acaatatcaa ctttcttaga atcaagaatc
      421 tttttcaatt catcagttac aaaaccttta atgccatatg acccatcatc ggtagtaaca
      481 ataatctggt cactttttgc tttcagttcc tgctccagta tcaataaatc tttattccgt
      541 gcaccaataa tgtttataca aaaatttccc gcttccttca ttgcaactgc tatagggtac
      601 acggcagcaa taccaatgcc accacccaca cacaccgctg ttccatagtt acgtatgtcc
      661 gtggggtgtc ccagaggtcc tgcaacatca tatacatatt caccttcttt tttgagcgat
      721 agtttatggg tagaggtccc cacaatctga taatacaggg taatggtacc tttttccctg
      781 tcagaatcag caatagtgag agggattcgt tcgcctacct catcaactat tacgatgaca
      841 aattggcctg ccttacggtg gtgtgcaata tacggggctt caattatagc cttgcccaca
      901 ttttccgata ttttactatt ttgtattatt ttattcacat gtacctcatt ttataaacat
      961 gaaattttat tatatattaa aatacttaat attttacagt ttttcaacat atatttttta
     1021 tcttgcagaa tagcctcatt gattacaatt atttactaat acaggaatgc aagaaaaaat
     1081 tattcttttc aaatttatct tcttatgtta catatatgtt aaattttaag gaatttatat
     1141 ttcatacaca ttaaatattt tgacttttaa accaatcaac cttcaataac acaaaagttc
     1201 ttaaaaatat ctttttgcaa acttgttact ttaaaaattt ttttgatcag cactgtcaag
     1261 caaaagctta aaaactataa caatttacaa aggagcacac ttatgggaaa tctatcacga
     1321 ccaaattcta acgatgcaac ccgtaccgct aaccgttcaa ggagtgtagc accacagtct
     1381 ggtatttgct cccggtgtct ggatggctgt acagggaatt gcgaaatttt ccaggccacc
     1441 atgcgaggac gcgaattgct atatccgggt ccttttggtg agataacagc aggggctgat
     1501 aaggattacc ctgttgacta ttcacacctc aacattcagg ggtatgcagc tggtgcaaag
     1561 ggtctactca atcctggccc tgaaactgca cggtttccat tagtttcaac agaaacgtat
     1621 tttggaacta aaaacagggt caaactggaa gtaccaatgt ttaccggcgc actgggttca
     1681 accgacatag cacgaataaa ctgggaacat tttgcagttg gtgcagccat ttcaggtatt
     1741 gctattgtat gtggtgaaaa cgtatgtgga atagaccccg gtctggaaag ggacaaaaat
     1801 ggcaaggtta ctaaatcacc tgaaatgatc cgtcgtgtag aaacctttaa aaaatataaa
     1861 ggtgaagccg gggatatcct tgttcagatg aatgttgaag ataccaactt aggtgtggct
     1921 gaatatatta tctcacagct tggtgttgaa accgttgaat taaaatgggg acagggtgcc
     1981 aaatgtatag gtggtgaaat aaaagttaga agtttagaaa gagctataga acttaaaaat
     2041 cgtggataca tagtaacacc tgacccaact atccctgcta ttcaagctgc tttcaaagat
     2101 ggtgctatta aagagtttga gcgccattca cgcttgggat tcatcagtaa agaagctttc
     2161 cttcaggaag tagaaagact cagagcattg ggagcaaaac gcgttacatt gaaaacaggt
     2221 gcgtactctc aaaaagaact tgcagctgca ttacgctggg catctgaagc aaaaattgac
     2281 cttcttacaa tagatggcgc tggtggtggt acaggcatga gcccatggcg catgatggaa
     2341 gagtggggtg ttcctacatt ctatctccag tgtatggcct atgacatggc tcagaaaatg
     2401 gcttcaaaag gcatgtgggt tcctgattta gcaatggcag gtggtttttc aactgaagat
     2461 cacatcttca aagttttagc aatgggatct ccatatttca aagctgtatg tatgggacgc
     2521 gcactgatga tacctggcat ggttggcaaa aatattgaaa aatggttaaa agaaaataac
     2581 ctgccaaaga atgttgctca gcatggcgca acaaaagaag agatctttgt atgctatgaa
     2641 gaactcaaag ctaaatacgg gaaagaagtg gataatttcc ctcttggagc aataggtctt
     2701 tacacattca gccagaaaat aaaagttggt ttacagcaga ttatggctgg cagccgcaat
     2761 ttcagaattg ataccatttc aagaagcgac cttatggcac ttacagaaga agcagcaaaa
     2821 atttctggaa taccgtatgt aatggatgcc tataaggaag aagctgaaaa gatcctttta
     2881 gaaggatgtg attacatcta atatttattg tatccattta aaaagaggcc gtctcaaaac
     2941 aaaccatggg gcggcttttt ttagtttttg atattttaaa aaaagggggt gtcttttgag
     3001 acacccccta ttttatctta tgaatgaaat atgtacttcc cgtcaaatgc ggcaagagcc
     3061 ttaattaatt tttactttat tcgtacatct tctcaattac atctttatag acttgaatta
     3121 tcacgcgttt ctttgctttc tgagttggag taatcatacc atttgattct gtaaacctct
     3181 ggttcagtat ggtgtagcgc ttgacttctt caaatttttc cagtcgtttg tttgcttctt
     3241 ttacttcatt atctatcagt tcctgcaaac gtttattatt gataaaatca tctccaactt
     3301 ttgtacatat ctgcatgcca ccaatatttt catataccag ttttgactca tcaaaagcaa
     3361 tgccttcacg tttgaacaac gctataaagt ttgcaaagtt tggcacaatc aacgcgctaa
     3421 tatatggctt ttcatcacct acaaggaaaa tctgttctac ccagctactt gttgaaaata
     3481 gattctcaat ttttgctggt gcaacatttt tgccaataga agtacagatt attccttttt
     3541 tcctttcaac aaatttaata tacccatcag gtgtcaactc tactacatca ccggttttaa
     3601 accaaccatc tttggtaaat gcctctttag tttcttttgg cttgttgaga tagcctttaa
     3661 atataccagc gcctgaaagc tccagttcac catcagcagc cactctacat ttactaccat
     3721 tggcttcttt acccatacaa ccaggcttgc acgcagtaat tggattaagc actgctgcat
     3781 tgaagctttc agttgaacca tatccttcta caacagccag ccccaatgca taataaaacc
     3841 tcagcaaatc aggcgatata gccgcacttg ctgaaaatga atggtggaat ctgtttccaa
     3901 ataaaccacg aaccttggca aaaagtttat cagctattga atactttatt ttaagaccca
     3961 aaggcaactt agattttaaa tcaaactctg gatgcatgtt gtagcaacca tttgcatctt
     4021 tgcggtattc aagtgcctgt aatcccacac caattgccca gtcaaacagc tttttcttca
     4081 atgtgctttc tgccattgcc tgctgcatgg ttatataaat tttttcatac aatcgtggca
     4141 cacagttaat ccatgtggga ttatattttt gcatatcttc aattaatgtt gcaggcttat
     4201 cagcatagca tatcaaagat cctttagtta aggcaagcca ctggcaagaa ccgcggtcaa
     4261 atacatgcga tagtggcagg tagcatagtg taacatcatg ttcattaaca tcaaaattat
     4321 atttctggaa atatgcaact gtaccctcta atcgtgatga accagtccag tgcgtgataa
     4381 tagcaccttt cccctgaccg gttgttccag aagtatacag tattgtgaac caatcatcca
     4441 attttacacc atctttccgt ttgatgtatt tcttataatt tgctgcattt ttccgccact
     4501 ctctgcctat ggcaataaaa tcctttatgc catagacctt cttgttgtca cctttaaatg
     4561 atgtgtccat tacaataaca gttttaagat ctttcagctg tttccagatc cgttttatct
     4621 gatccaattc ctctttgttg ccaacaaata tcactgtaga ttttgaatct ttcatgatgt
     4681 atgtgacttc agtatcagat aatgtgggat aaattgttac actgacgcca ccagcacagg
     4741 caatcgcaat atcaacctgt gtccacatat agcttgttgg cgccataatt gcagctctgc
     4801 ctttgtggag aaaccctatt ttcaacaatg cacaagcaat atcctctaca cgctctttta
     4861 actctgcata cgtaaacatt ccattattgt catcatacag cttactatta aatttttgtg
     4921 caggccgttc aggaaatttg ctgcaagtgt cataaaaata ctgaaccatc gttttaagct
     4981 cacccataat aacccctctg aatatattaa tttaaatata aatatttata tatcacgact
     5041 ggaaggtatt tcaataaatt aattacattt ttccatttat gttatttact gctatgcaat
     5101 acaatttccc tgcattttgc aagtgctttt gcaatatgtt caggggattt gccaccagcc
     5161 tgtgccagat cttttctgcc gccaccaccg ccaccaatct cttttgatac cgttttgata
     5221 atagcaccac aatcaattcc tttatctata gcacctttgg tagctgcagc tataataaat
     5281 gccttatttt cctttgaaga agctaaacac actaccaaag tatccgaaag ctcccgtata
     5341 taatcagcca gtgtacgtaa agcttcagca tccatatttt gcagtgacgc actcacaaac
     5401 ttcagactgt tgatatgttc agctttggta tataactccc tggcaatatc tttcaaccct
     5461 gcagcttttg ccttttccag ttccttttcc agataggaaa ctttttcaag catttcggca
     5521 accttcttct caatatttga ttcatttact tcaagcaacc ccattatcct tcttactatc
     5581 gcatgctcat gaatcagccg ttcatatact cccttcagtg tcactccttc aattcttcgc
     5641 acacctgcac caggggaaga ctctcgcatt attctgaaaa caccaatttt gcctgtattg
     5701 tcaacatgcg tcccaccaca taattccttg ctgaaatcgc tgatagaaac cattctgaca
     5761 atatcagaat atttttcatc aaatactgcc atcgctccct ggtttaaggc ttcctgcaat
     5821 ggtataattg cagtattcac cggcaatgca gcccatattt tttcattaac tatcgcctcc
     5881 acctgctgaa tttcttcctg tgtcattgcc ttaaaatggg aaaaatcaaa cctgaaatgc
     5941 tggggatcta ccagtgaacc tgcctgcttt acatgctgcc ccaaaacgtg acgcagtgct
     6001 gcctgtaaaa gatgtgttgc ggtatgattt gctcgggtaa gatttcttct gagaccatca
     6061 attgataacc gtacctttgt accttcttga agagttcctt tttccacctg ccctatatgg
     6121 acaatcagat caccagattt ttttgtgtcc acaaccctga atgttccact atcaccaata
     6181 atgtaaccag tatccccaac ctgcccacct gactcaccat aaaatggcgt gtttttgaca
     6241 accagtattc cttcctgccc ctcatttaaa tgagtcttta agctttcaaa atctgacagc
     6301 acaataattt ccgattctga atcatatttt tcatagcctt caaactgtgt gctctgaacc
     6361 ttttttaata tttcacctac aaccccatca atcctgcctt ccacaccttt ccagctctgt
     6421 cttccgcggt tacgctgttc ttccatttta t
//
LOCUS       DAOSZP010000042         6409 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_10265_length_6409_cov_3.246931, whole genome shotgun sequence.
ACCESSION   DAOSZP010000042 DAOSZP010000000
VERSION     DAOSZP010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6409
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10265_length_6409_cov_3.246931"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            471..671
                     /locus_tag="PK348_01695"
     CDS             471..671
                     /locus_tag="PK348_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003012710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HXK64956.1"
                     /translation="MQKNMGTIDRLLRTIIAIVIIVLYFTGQITGTAAIILGILAVVF
                     LLTSAVSFCPLYVPLKINTKKQ"
     gene            complement(757..1356)
                     /locus_tag="PK348_01700"
     CDS             complement(757..1356)
                     /locus_tag="PK348_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010479675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HXK64957.1"
                     /translation="MKHDIILPKPLYEGNVSVEYALKQRRSVRRFSKMAISLQELSQI
                     LWAGSGISQGSYRTAPSAGATYPLNCYVAAGNVEELEKGVYRYIVKNHGLKRIAPYDL
                     RSALCRAALHQTFIEEAPIVIVITAIYERTTGHYGQRGIRYVHMEAGHAGQNISLQAV
                     ALGLGSVMVGAFQDKMVKEIVSLEKGEEPLYIIPVGKPA"
     gene            1623..2738
                     /locus_tag="PK348_01705"
     CDS             1623..2738
                     /locus_tag="PK348_01705"
                     /inference="COORDINATES: protein motif:HMM:NF019522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HXK64958.1"
                     /translation="MKKIFFVVLLLTITIPLFAKMQYDDERIFTRAEITKIEKHRIAG
                     DHNYPLYETKIHVTILDGVLKGQTKVATFRGEHDLPKDMFYKQGDVVFIDISKTGMES
                     TPEYISLYDIDNTTMILILTLMMILSVIVIGKVKGFFSLMALLITILLLFFVLIPLTL
                     KGYPPLPLAIVISIISTVITLPVIAGFKKKTFAAILGASAGIIIASILAYIVGNIMHL
                     SGFVTNEMLTVFYVADVAIDIRGLALASIIIAALGAVMDVCISIASATAEIFNANPDL
                     SPKDAFRSVLTVGTDILGSMVNTLVLAYVGSSLSLILLISMRIQPGMPVWMIFNYNPI
                     LSEIVKSIIGSIGMFICIPITAYISVHLYGRKDGTAR"
     gene            complement(2782..4686)
                     /locus_tag="PK348_01710"
     CDS             complement(2782..4686)
                     /locus_tag="PK348_01710"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HXK64959.1"
                     /translation="MIHKWYIQQLTFPEILNRNAIQFGTRRAQWWKTGPNTTTSITYA
                     ELGIIIKQLTAGLMSLGVEKGDRVAIASHTAPQWLWADYSILCAGGITVCVYPTLSAK
                     EMSFIINDSGSKILYVQDEEILLRALSAIQEMPTLEKIIIMNNDYNGNDSRVVTLQQV
                     RDFGIKLLAQDRLAYERRWRSVNILDPMTIVYTSGTTGRQKGAVHTHFSINAACCRDL
                     RIVPEYREDDVLLSFLPLAHTYERECGHGTAMMAAATIAYSSPMTLVEDLQIFRPTVF
                     MSVPRIYERIFMAMRDMAAKSPIKKKIFDFAIKTGLQLTEARADKNGFVDMSEGIDFT
                     EGVGRWLAFKYKLVDKLVFSKVREKLGGRFRFAFSAAGSLPADLCKVFMAMGVRIYEG
                     YGATETCNTVNLNLPHKVLPGYVGPVCNGVEGRVASDGEWQVRGDNIFLNYWNNPEAT
                     KEAFTEDGFYKTGDIVQMGPDGYIKIVDRKKGIMVLDTGKNVPAAKIESLFSVSRYVD
                     IVFPIADDRPFVGALVVPKFDVFIELFDKEGIPYDKSKLVFFGEGAARMCIGVGEDFI
                     QNEKLKKLVDEDIQLANRELEEYERIKKYVIINRKFTEATGEMTPTLKVKRNIIMKNF
                     EKEINELFKR"
     gene            5106..5831
                     /locus_tag="PK348_01715"
     CDS             5106..5831
                     /locus_tag="PK348_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK64960.1"
                     /translation="MMDAQPIRFVVTSNTSPDSPYEEFNPQLTNTLLSINKENPVFTM
                     HCGNIIHGGSSIMGINVIDVEKQFQQFFKFAKKLNCILFTACGPLDTYNDSTAIYTKF
                     TGKDAWYSFNYGTNHFIVLYSTSKKTLSISDRQLSWLKEDLKKHSHAHIFVFSYFLPD
                     IQRTKQQFANDSHQKLHELLSQYTVKAFFGVTGLTQPVTIGTIQYIPVPCDNSYTNKY
                     KKAIRYYVVDFDGQYITLVPRYF"
     gene            5850..6131
                     /locus_tag="PK348_01720"
     CDS             5850..6131
                     /locus_tag="PK348_01720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK64961.1"
                     /translation="MQREIKFDSIHEFINALHASNITKAAFTQINEKRAVEVKPGTLQ
                     VVDVVIAEILAYKDATIYKCKIENADLDDLYTMLEDEGFELTKRSRNIT"
     gene            6134..>6409
                     /locus_tag="PK348_01725"
     CDS             6134..>6409
                     /locus_tag="PK348_01725"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXK64962.1"
                     /translation="MEAIVTIENLTISFTNLYGKSYAVRNLSLSIPKGKTLGLVGESG
                     CGKSVTAYSILNLVPKPGKIESGTITYNSIDLLHCSETTLRSIRGKNI"
BASE COUNT         2008 a         1339 c         1041 g         2021 t
ORIGIN      
        1 ccgcaaaagc ctgaatcgtt ggtagtacta tcgtaatcac aagagcacct atcaaggttt
       61 taaacgatat tacatgccaa acagtactat tgtttccctt tctcatgatg gatacaatgc
      121 ttgttaacac tatttaaaat accctgtact ttatattcat tttgattaca gcactatttc
      181 tttacaaatt acccatgatt gtaaattgat taatacacat tgaatgatgc acaacgtaca
      241 cctgagaagc tggaaagtat acatgccatt attcattcaa tcttttagta ttgatgttag
      301 ccacaataaa aaatcactct atgttcagtt tgtactcatg tcaagggatt attataagaa
      361 aggacagacg ttttaatttt cctcaatgca aaaaaaacat taccacacct tgacttttaa
      421 tcaatattta agtataatac tatatactat aatactacag ggggataaca atgcagaaaa
      481 atatgggaac aattgacagg ttactgcgaa caataattgc cattgtaatc attgtgcttt
      541 actttaccgg gcagattact ggcactgcag ctattatact gggaatactt gccgtggttt
      601 ttttattgac cagtgcagta agcttttgcc ctttatatgt tccactcaag attaatacta
      661 aaaaacaata atcgagttaa cgttaaccca aacacaattc aggaattatg actatataat
      721 tgaataccca aaaaaagggg tattcaatta tgtatgttat gcgggtttcc caacaggaat
      781 aatatagagc ggctcttcac ctttttccaa tgatactatt tctttaacca ttttatcttg
      841 gaaagcccca accatgacac tacccaatcc cagtgcaaca gcctgtaaac taatattctg
      901 gccagcatgc cctgcctcca tgtgtacata ccgtataccg cgctgaccat aatgccccgt
      961 agtgcgttca tagatagcag ttattacaat gactattggt gcttcttcaa taaacgtttg
     1021 atgtaaagca gccctgcaca gggcactacg caaatcatac ggtgctattc gtttcaatcc
     1081 atgatttttt acaatatacc gatacacccc tttttctaat tcttcaacat tgccagctgc
     1141 tacatagcag tttaagggat aggttgcacc tgctgatggc gcagtacggt aacttccctg
     1201 tgatatgcca ctgccagccc atagtatttg cgatagttcc tgcaaagaga tagccatttt
     1261 agaaaaacgc cgcaccgaac gacgctgttt cagcgcatat tctactgaaa cgtttccctc
     1321 atacaaaggt tttggtaaaa taatatcatg tttcataact catatcctgc aattaaatag
     1381 cagacattca ttcttgggtt agcactcatt tcactgctta ataatagaaa acattattcc
     1441 actctaacag attaaaaaat acaaattgca acttgacaaa tcaacacatg catcaactga
     1501 tataattaaa aaattgatat gtacttccaa ttctcacatc tgtggcagtt cataatgttg
     1561 tattgttttt cattacgcta tttgatttca atttaattat aataactctc aggaactatc
     1621 tcatgaaaaa aatatttttt gtagtgttac tgttaactat aactatacct ttatttgcca
     1681 aaatgcaata tgatgatgaa agaattttta cccgtgctga aataacgaaa attgaaaaac
     1741 accgcatagc aggtgatcac aattatcctc tatacgaaac aaaaatccat gttactatcc
     1801 ttgatggcgt attaaaagga cagacaaagg ttgctacctt ccggggagag catgacctgc
     1861 caaaagacat gttctataaa cagggggatg tcgtattcat tgacatatcc aaaactggaa
     1921 tggaaagcac tcctgaatat atatcgctat acgatattga taatacaaca atgatactaa
     1981 tcctcacatt aatgatgata ctatccgtaa tagtcattgg aaaagtaaaa ggcttttttt
     2041 cgctgatggc tttactcatt acaatactct tactattttt tgtattaata cccttaacac
     2101 ttaaaggcta tcccccttta ccattagcaa tagttatttc aattatatcc actgtgataa
     2161 cattgccagt aatagctggt ttcaagaaaa agacatttgc tgctatatta ggcgcatctg
     2221 caggaataat tatagcatct atccttgcat atattgtggg aaatataatg catctttcag
     2281 gatttgttac caatgaaatg cttaccgtat tttatgttgc cgacgtagca attgatatac
     2341 gggggctggc attagccagt attatcattg ctgctctggg tgcggtaatg gatgtatgta
     2401 tttctatagc gtctgcaact gctgaaattt tcaatgcaaa tccagattta tcacctaagg
     2461 atgctttcag gtcggtttta acagtaggaa ctgatatttt gggttccatg gtcaatactc
     2521 tggtcctggc gtatgttgga agctctctgt cattaatact tcttatctcc atgcgtattc
     2581 aaccaggaat gccagtgtgg atgatattta actacaatcc aattttaagt gagatagtta
     2641 aatcaattat tggtagcatt ggtatgttta tatgcatccc tatcactgca tatatttcag
     2701 tacatttata tgggcgaaag gatggcactg ctagataaat ttcaccccac catgtaaagc
     2761 tggtggggtg aaatagaaga actaccgttt aaacaactcg ttaatttcct tttcaaaatt
     2821 tttcatgatg atattacgct taactttcag cgttggcgtc atctctccgg ttgcctcagt
     2881 aaacttcctg tttatgatta catatttctt tattctttca tactcttcaa gttccctgtt
     2941 tgccagctgg atatcctcat caaccagttt tttcagtttt tcattctgaa taaagtcttc
     3001 gccaacacca atacacattc tggctgcgcc ctcgccaaag aataccagtt ttgatttatc
     3061 gtatggtatt ccttccttat caaacagttc aataaaaaca tcaaatttag gaacaaccag
     3121 cgcgccaaca aatggcctgt catcagcaat tgggaatacg atatcaacat accgcgagac
     3181 ggaaaataaa ctttctatct ttgcagcagg aacatttttg ccagtatcca gaaccattat
     3241 cccttttttt ctgtcaacaa tcttgatata tccatcaggc cccatctgaa caatatcacc
     3301 tgttttatag aaaccatctt cagtaaaagc ttctttcgtt gcctcaggat tattccagta
     3361 attcaggaat atattatcac cacgcacctg ccattcgcca tcacttgcca cacgcccttc
     3421 tacaccattg catactggcc caacatagcc aggtaatacc ttatgcggca aattaagatt
     3481 aacagtatta caggtttcag tagcaccata cccttcataa atcctgaccc ccattgccat
     3541 aaacaccttg cacaaatctg cgggcaaact gccagcagct gaaaacgcaa acctgaatct
     3601 gccgcccaat ttctcccgta ccttgctgaa tacaagctta tcaaccaatt tgtatttgaa
     3661 tgccagccac cttccaacac cttctgtaaa atcaatgcct tcggacatat caacaaatcc
     3721 atttttatct gcacgtgctt ctgtgagttg caatccggtt ttaattgcaa aatcaaagat
     3781 cttcttcttt attggggatt tagctgccat atcgcgcatt gccatgaaaa tgcgctcata
     3841 tattcgcgga accgacataa aaactgttgg cctgaaaatt tgcaaatcct caaccagcgt
     3901 cataggcgat gagtatgcaa tggttgcagc agccatcata gctgtaccgt ggccacattc
     3961 acgttcatag gtatgtgcaa gcggtaaaaa tgaaagcaac acatcatctt cacgatattc
     4021 cggaacaata cgcaaatccc tgcagcatgc tgcattgata ctgaaatgag tatgcacagc
     4081 acctttttgc cttccagtgg tgcccgatgt atagacaata gtcattgggt caagtatgtt
     4141 aaccgatcgc catcgccgct catacgcaag cctatcttgt gccagcagtt taatcccaaa
     4201 atcccttacc tgctgtagcg ttacaacacg tgaatcatta ccgttgtagt cattgttcat
     4261 gatgatgatt ttttccagtg ttggcatttc ctgtattgca ctcagggccc gtaatagaat
     4321 ttcttcatcc tggacatata aaattttaga tccggaatca ttaataataa aactcatttc
     4381 ttttgctgaa agcgttggat atacacatac agtaatgcca ccagcgcata gtattgaata
     4441 atccgcccat aaccattgtg gtgcagtatg ggaagctata gcaacacggt cacctttttc
     4501 cactcctaag ctcatcagac cagcagttaa ctgttttatt ataattccta actctgcata
     4561 ggttatggat gttgtagtat ttggaccggt tttccaccat tgtgcacgcc gggtaccaaa
     4621 ctgtattgca ttcctgttga gtatttcagg gaatgtcagt tgttgtatat accacttatg
     4681 tatcataaaa gcccccttat aattaataag aaaatctgga aaatacatag catattttat
     4741 tacgataata tatttatttg tatatgtcaa gttgaatatt ataattatta ttttatacag
     4801 caaaaaaatt tttactgttt cactaattct ggaaaattga aataataaca atagattaat
     4861 tgagtttatt ctacatatca aacatctttt tgccggttta tcaaaatatt tattgataaa
     4921 aataaaatat tagtatgcaa catcctattg tggtattact gtatattaat ggtattattg
     4981 ttcaatattc ctgatatatt acctgaacaa cggtgattgc tactatgaag aaactatctc
     5041 ttgctatctt ttttgtgata acaacagttt tcataatcat tcagtgctcc tgcagttcca
     5101 gccaaatgat ggatgcacaa cccatacgtt ttgtggttac cagcaacaca tcgccggatt
     5161 cgccctatga ggagttcaat cctcagctta ctaacacact tctatcaatc aataaggaaa
     5221 acccagtatt taccatgcat tgtggcaaca tcattcatgg gggctcatct atcatgggta
     5281 ttaatgtaat tgatgttgaa aaacaattcc agcaattttt caaatttgca aagaaactta
     5341 actgcatcct ctttactgca tgcggtccgc ttgatacata taatgattct acagctatct
     5401 acacaaaatt taccgggaag gatgcatggt attcttttaa ttatggcacc aatcacttca
     5461 ttgtattata cagcaccagc aaaaaaacac tttccatttc agacaggcaa ctgtcctggc
     5521 taaaagaaga tctgaaaaag cattcccatg cgcatatatt tgtcttttca tactttttgc
     5581 cagatattca gcgcacaaag cagcagtttg caaatgattc acaccagaaa cttcatgaac
     5641 ttttgtcaca gtatactgtt aaagcttttt ttggcgtcac agggcttact caaccagtaa
     5701 ctatcggcac tattcaatat attccagttc cctgtgacaa ttcgtatacc aataaatata
     5761 aaaaagcaat acgctattat gtcgttgact ttgacgggca atacattacc cttgtaccac
     5821 gatattttta aatcatataa ggtcatgcca tgcaacgtga aataaaattt gattccatac
     5881 atgaatttat caatgcactt catgccagca acattactaa agcagcgttt acacagatta
     5941 acgaaaagcg tgcagttgaa gttaaacccg gcacattaca ggttgtagat gttgttattg
     6001 ctgaaatact tgcctataaa gatgcaacca tttacaaatg caagattgaa aatgctgatt
     6061 tagatgacct ctatacaatg ctggaagatg aaggttttga attaacaaaa cgaagcagga
     6121 atattacata atcatggaag cgatagttac cattgaaaac cttaccattt catttacaaa
     6181 tctttatggt aaaagctatg cagtacggaa tctgtcactg tccataccaa aaggcaaaac
     6241 gttgggcctt gtcggtgaat cagggtgcgg taaatccgta actgcatatt ctatccttaa
     6301 tttagtgcca aagcctggca aaattgaatc tggcactatc acatacaatt ccattgatct
     6361 tttgcattgt tctgaaacca cactgaggtc aatccgtggg aaaaatata
//
LOCUS       DAOSZP010000043         6305 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_10466_length_6305_cov_2.678400, whole genome shotgun sequence.
ACCESSION   DAOSZP010000043 DAOSZP010000000
VERSION     DAOSZP010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6305
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10466_length_6305_cov_2.678400"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(196..2274)
                     /gene="fusA"
                     /locus_tag="PK348_01730"
     CDS             complement(196..2274)
                     /gene="fusA"
                     /locus_tag="PK348_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011365698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HXK64963.1"
                     /translation="MPLKKLRNIGIAAHIDAGKTTVTERILYFTGVTRKLGEVHDGQA
                     VMDFMKQEQERGITISSAAISCQWNGHSINIIDTPGHVDFTVEVERSLRVIDGLIAVF
                     CAVGGVEPQSETVWNQADRYKVPRIAFINKMDRVGADFYEVVSQMDKYLDANPVPFQI
                     PIGAEDNFEGCIDLVEQKAIVFNENERLVVDIPQDYKDKANEARKNIIEKLADYDDEI
                     MELYLAEQEVPAELIKKVGREATLKMLITPVLCGAAYKNKGIQLLLDAVVDYLPSPVD
                     IGAVVGTDLNDSEKTHTRHPSVNEPFAALAFKLINDLYVGQQTFIRIYSGKLESGMQV
                     FNSTKGKYERIGRIMRIRAKDREEISEAGPGDIVALIGMKYTKTGDTLCDPNHPLLLE
                     SIFVPPSVIDLQIMPTSKKEEEKLGIALHKLASEDPSFNVRVDEETGETIISGMGELH
                     LEIMVDRLKEEFGLNVQVGEPSVAFRETITTEAQQETKFVKQTGGKGQYAHCVIRIEP
                     NPAKGFEFVDHIKGGAIPNEYIPSIQKGIHAAMEEGILAGFPVVDVRVVLLDGSFHPV
                     DSSDMAFRTCASICFKDAFMKAKPILLEPQMKIEVNTPDEYIGDVIGDLNKRRGKVEA
                     MRRFRKGAQKVNGFVPLMEMFGYATTLRSLSSGRANYAMEFDKYVPLPREMQEKVLAK
                     IKEKKRKQSA"
     gene            2629..4077
                     /locus_tag="PK348_01735"
                     /pseudo
     CDS             2629..4077
                     /locus_tag="PK348_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007884251.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4365..5159)
                     /locus_tag="PK348_01740"
     CDS             complement(4365..5159)
                     /locus_tag="PK348_01740"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HXK64964.1"
                     /translation="MGNRSDKPMNPLEMLAYILSKQIKDHEVVYIGTGLPMIAGILAR
                     KTHAPNIVLVYESGAQDPMEGALPWSVGGPATWRKSPLIMEMAYSFGQLYNGYVDKAF
                     LGFAQIDMYGNVNTHMIGKDFLKPDHRLTGSGGNNDLSSLAENMILVGLQSPQKFVPK
                     VDFITSPGFLDGGDSRKKAGLLGNGPVCVISQAAVFDFEPETKRMRVKSLHPGVSFDL
                     CQLACGFELLKPEGEIPVTEVPSKEILEILRNEVDPRGVFTSIPGM"
     gene            complement(5197..>6305)
                     /locus_tag="PK348_01745"
     CDS             complement(5197..>6305)
                     /locus_tag="PK348_01745"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HXK64965.1"
                     /translation="KFSFNEEAVKNLKITDTSILERLVDFEDARKFHLNKDRSRKDKR
                     MSLKEAVAEYVHDGDIMTDGGFCYVRTSIQSYFEVIRQQKKNLQFCSSPNTNHSYMIN
                     AGCCRYSHNSYLGAEMRGIDRNYARQLKAGKVTILSEWSHGTMAQGFKAAQLGLPGVF
                     SKQLLGSDMLKYNPYVKVMQNPIRSDADPVVFIPAIYPDVSIIHVHAADKKGNARFFG
                     PAINDIAMAAATRKLIITAEEIVPESDIRYNNKGVYIPAMYVDAVVELPFGAVPGQMP
                     GCYYWSRQWWEMAFRYAMKSDENFQHFLEYWVYSVKDQYEFIEKLGGAKWIAESRRLT
                     KAEEYDNEDIGVNFDYEEYASGEWPEDMSKMRFY"
BASE COUNT         1881 a         1390 c         1077 g         1957 t
ORIGIN      
        1 aatagccatc agcagaaagt ctatatggat gcaggtaatt gaaaaaaaca tcctccattc
       61 accacttgaa atatatacag cactggaaat caataataga attatcctgt tttacgacaa
      121 gattacatat ttcataatga aaggatatga acatttaata taaaaaaagg ggcactttta
      181 catgcccctt tatatttatg ctgattgttt gcgtttcttt tcttttattt ttgcaagcac
      241 cttttcctgc atttcacgtg gtagtggaac atacttatca aattccatag catagttagc
      301 tcttccactt gaaaggcttc gcaatgttgt tgcataccca aacatttcca taagtggtac
      361 aaaaccatta accttttgtg ctccctttct gaaacgccgc attgcctcaa ctttaccacg
      421 ccttttattt aaatcgccaa taacatcgcc aatgtattca tcaggggtat tgacttctat
      481 cttcatctgc ggttcaagta aaattggctt ggccttcata aatgcatcct tgaaacatat
      541 cgatgcgcat gtcctgaatg ccatatctga tgagtcaaca ggatggaagc tgccatcaag
      601 aagaactact ctgacatcca caacagggaa tccagctaaa ataccttctt ccattgcagc
      661 atgtatcccc ttctgtattg aaggtatgta ttcattaggt atagctccac ctttaatatg
      721 gtccacaaac tcaaaacctt tcgcaggatt tggttcaatt cggataacac aatgtgcata
      781 ctgaccttta ccacctgtct gcttaacaaa tttggtttcc tgctgcgctt cagtggttat
      841 ggtttctcta aatgctactg acggttcacc cacctgtaca ttcagaccaa actcttcttt
      901 taacctgtcc accataattt ctaagtgaag ttcacccata cccgaaatta tagtttcacc
      961 cgtttcttca tcaacccgca cattgaatga cgggtcttct gatgctagtt tgtgcagagc
     1021 aatacctaat ttttcttctt cttttttaga agtgggcatt atctgcaaat caataactga
     1081 aggcggcaca aagatggatt caagcaacaa cggatgattg ggatcacata atgtatcgcc
     1141 tgttttggta tatttcattc caattaatgc aactatatca ccgggacctg cctctgaaat
     1201 ttcttctctg tctttggcac ggattctcat tattcggcct attcgctcat atttcccttt
     1261 tgttgagtta aatacctgca tcccgctctc aagctttcca gaatatatgc gtataaatgt
     1321 ctgctggcct acatacagat cattaattaa tttaaacgct aacgcagcaa atggctcatt
     1381 aaccgaagga tggcgtgtat gggttttttc cgaatcattg agatcggtac ctacaactgc
     1441 accaatatca actggcgaag gtagatagtc aacaactgca tccagtaaaa gctgtatgcc
     1501 tttattctta tatgcagcac cacataatac cggagttatc agcattttta atgttgcttc
     1561 acgcccaact ttttttataa gttcagccgg tacttcctgt tcagctaagt acagttccat
     1621 tatctcatca tcataatcag ctaacttttc aataatgttt ttgcgggctt cattagcctt
     1681 gtctttataa tcctgtggta tatctacaac aagcctttca ttttcattga acactattgc
     1741 tttttgttca acaaggtcta tacagccctc aaagttatct tcagcaccaa ttggaatctg
     1801 aaatggcacc gggttagcat ctaaatattt atccatctgc gacacaactt cgtaaaaatc
     1861 agcacccaca cgatccattt tattaataaa tgcaatacga ggtactttat acctgtcggc
     1921 ctgattccat actgtttcac tctgaggctc aacaccgcca accgcgcaga atactgcaat
     1981 caatccatca ataacacgca aagaccgctc cacttcaacg gtgaagtcaa cgtgtcccgg
     2041 ggtatcaata atattgatac tgtgcccatt ccactgacat gagatagcag ccgaggatat
     2101 ggttatcccg cgttcctgtt cctgtttcat gaagtccatt acagcctggc cgtcatgaac
     2161 ctcgcccaat ttccgtgtta ccccggtaaa atataaaata cgctcggtaa ccgttgtttt
     2221 gcctgcatct atatgcgcag ctataccaat atttctcagt tttttaagtg gcatagtttt
     2281 ttactcctta caatataata atttcattat cagcaaaatg cttagctgac cgcttattac
     2341 tatagtggaa ttaatgttcg ctttttttta atctaagagg aagaaaacca ctaaatatgg
     2401 tcggtcatag tgataaagat agcaaacctg tcaacaattt taaaattttt tttatatttt
     2461 aaaattaaaa cttgcaatat tttgttgttt tcggttaatt tattacggta actatcgccc
     2521 caaaatattt gtgcgatagt tactgtttaa ttttgtttta ttccccaaaa cggcggaatt
     2581 ctttgcaatg aaatccaata ctgaattcaa tttttatgga ggcgatacat gacaggcgac
     2641 agatctcaat ctatttttgg caatcagatt tccaaacatg tgtacagcaa agctgtaaag
     2701 cagaaagaac gttttgcaaa acaatttgga tataatccac aggataccta tctgctttat
     2761 gctcaaccaa acccggtatt aaaaaaatat gttaacctgt acaccattgc gcaggaaaag
     2821 ggggcagaaa ttgcaaaagc aaaatcaatc attattggta ccatacgcat gggttatgga
     2881 cattaccgaa ttgctatggc agttgcatca gcagcacatt ctatgggatt gacaccatac
     2941 tggtttgacc ttctttcatt tgatactacc ggtgccaaaa tcatcaaaca tttagaaaag
     3001 ctgtattcac tgggctcccg cctttcacag caattttatt tgtttaataa attgtactag
     3061 gagcatctca ccgcaattgg gtttaaacgc ctgccctata atgccagtga ccagaaaatg
     3121 accgagcttt ttgcaaatat atacgaaaat cttccccact ctgtgccttt tgttgctaca
     3181 catgcatggg catctcaggc tgccattcat gctggtatga aacgtgttgt caacatgata
     3241 cccgacaact ggcctctggc actgcatctt tccgaaggag ctctacacac cgtgcagaca
     3301 ccatctgcct attatggata caggacatta aaaaatatgg gcaagcgcaa tgaaatctta
     3361 aatcctatgc caaaagacag tttgtattat acaggccatt atattgacca cgaactggtt
     3421 gccaatattg aaaaagactg tactgcacgc ataaacagaa tgaaagcaaa aaagccacgc
     3481 cgatttttat taagcattgg tggcgctggc gcacagcaaa aactgtgtat ggatatcatc
     3541 cagcattgca caccgctact ggaaaatgaa aaacttacac ttattataaa taccggtgac
     3601 cataaaaaca tttttgatat gatagtcaat acacacttta gcccaaaaat tcaatgtaaa
     3661 atttacactc agtggacaga tacagaaaaa ttggtaaaca ccttgagtgc aaaggatatt
     3721 ccaggacttc atgtggtata taatgaaaat atattttccg ctgtgtatgc cacaaatctt
     3781 ttaatgcgag tgtcggactg ccttatcaca aagccaagtg aactggcatt ttacccagta
     3841 ccaaagcttt ttgttgcaag ggttggtggt catgaaatgt ggggtgcaat acgtggtgct
     3901 gaagttggcg atagtaccgt tgaatgcgaa acaaccgagc atacattgca ggcacttgat
     3961 ttgttgatgt atgatgatga ccttctttca atgtactgca ataatattat caaggcaaag
     4021 agtattggtg tgtacaatgg agcgtacgaa gttattaaac tggcattagg gaaataaaaa
     4081 atatattaat tttttaccaa taacactgtc attgatatga gcgtcagcta tcgatgccgt
     4141 gttgatacat tctttgcttt gctcagaaca ctgggtgacc tggtgtctta aagccggtca
     4201 ttgaatgcac tcgaagagta ttgaatactg ccataatctt ttcacatctc tggaaatgag
     4261 aagcttactg aaactgcaca tatataacat ccatcctgta gggaaaaaga aaacctctgt
     4321 tttgatgtac agaggttttc ttttaaattt cttataccaa gtacctacat tcctggaatg
     4381 cttgtaaaca ctccgcgtgg atcaacttca tttcgcaata tttcaagaat ttcctttgat
     4441 ggaacttcag ttacagggat ttccccttca ggtttcagca attcaaatcc acaagctaac
     4501 tggcatagat caaatgatac tcccggatgc aatgatttga ctctcatccg ttttgtttca
     4561 ggttcaaaat caaataccgc agcctgagaa ataacgcata caggtccatt gcccagtaac
     4621 cctgctttct tcctgctatc gccaccatct agaaatcccg gtgaagtaat aaaatcaact
     4681 ttgggaacaa atttttgtgg tgattgcaat ccaacaagaa tcatattttc agccagtgaa
     4741 gaaaggtcat tattcccacc tgaaccagtc aagcggtgat ctggtttcaa gaaatcttta
     4801 cctatcatgt gtgtattgac attgccatac atgtcaatct gtgcaaaacc caaaaatgct
     4861 ttatcaacat agccattata tagctggcca aatgaataag ccatttccat tattaacggc
     4921 gacttgcgcc atgttgctgg accaccaacc gaccatggaa gtgctccttc cattgggtcc
     4981 tgtgcacctg actcatatac caggactatg ttaggggcat gtgtcttgcg tgccagtata
     5041 ccagcaatca ttggcaaacc cgtcccaata taaactactt catgatcttt aatttgcttt
     5101 gataaaatat atgcaagcat ttctaatgga ttcattggtt tatcagatct atttcccata
     5161 agtcctcctt aatatttaaa tatttaaaaa taaattttaa tagaatctca tcttgctcat
     5221 atcttctggc cattcaccac tggcatattc ttcgtaatcg aaattaacac caatatcctc
     5281 attatcatac tcttctgctt tggttaatcg gcgtgattca gcaatccatt ttgcaccacc
     5341 caacttttct atgaattcat actgatcctt tacactatat acccagtatt caaggaagtg
     5401 ctggaagttt tcatctgact tcattgcata tctgaaagcc atttcccacc actgccgtga
     5461 ccagtaatag caaccaggca tctgacctgg aaccgcacca aaaggcaact caacaactgc
     5521 atcaacatac atagctggta tatatacacc tttgttatta tatcgaatat cactttcagg
     5581 gactatctct tctgccgtaa ttatcagttt tctggttgca gctgccatag caatatcatt
     5641 tattgctggt ccaaagaagc gagcatttcc ttttttatct gcagcatgaa catgaataat
     5701 tgaaacatcc ggataaattg ccggaataaa taccacaggg tccgcatcac ttcttattgg
     5761 attctgcata actttaacat acggattata tttcagcata tcagaaccta acagttgctt
     5821 gctgaatacc cccggcaaac ctaactgggc agctttaaaa ccctgtgcca ttgttccatg
     5881 gctccactct gaaagtatag tgaccttccc agcttttaat tgacgggcat agttgcggtc
     5941 aatacctcgc atctcagcac ccaggtaaga attgtgcgaa taacggcagc atcctgcatt
     6001 aatcatatat gagtgattgg tatttggtga actacaaaac tgcaggtttt tcttctgctg
     6061 tcgtataact tcaaaatatg attgaataga agtgcgaaca taacaaaagc cgccatcggt
     6121 catgatatca ccatcatgga cgtattcagc aaccgcttcc tttaaagaca ttcttttatc
     6181 tttgcgcgat ctgtccttat ttaaatgaaa ctttcgcgca tcctcaaaat ctaccaatcg
     6241 ctcaagaatt gaggtatctg taattttgag atttttaact gcttcttcat taaagctgaa
     6301 ttttc
//
LOCUS       DAOSZP010000044         6300 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_10482_length_6300_cov_3.230745, whole genome shotgun sequence.
ACCESSION   DAOSZP010000044 DAOSZP010000000
VERSION     DAOSZP010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6300
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10482_length_6300_cov_3.230745"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(120..3074)
                     /locus_tag="PK348_01750"
     CDS             complement(120..3074)
                     /locus_tag="PK348_01750"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXK64966.1"
                     /translation="MNIKEFNYSSITQRRFVLLSVFFSIATLVVFTLLNILYVSSLTL
                     YSPVWKYTLTFAFSGLLAGIIAGNSILKRLQNHRIIFIIQEFIAVCVAIAPVIAAFVY
                     NDILNAYGTILQHCYWLLYGAIFLLYFSTGIKAPYYLKTTIGDYIDDKNPAILAVCFA
                     LLSFIASLAFGLFIFYNQHYLILYVVLPILSCVFTILLSLPFCPPAIISRGVAEERAI
                     TSRDDILFTYCNVSFISIFIILILLSIVSRYGLYLQIIFYSAIAIAAGFIVGTIASQY
                     IKTHNTVFIYGEMLYPFLFLGFIIIIYSNDIPYYISIMTALPFSIFSGLTFGTSLKQI
                     ILKNNHQSRYTILLFSFFIIPIPIIIAFLWIELTYLWFFILIYLMAIITIIFPGMYLL
                     QNTKRSVPSILYFVVSLLFIPAIIITHIYFRLPFSNTLVNRLCTGYDTVAMANFNASY
                     ITQPATAYIQGIPACVVTDHFARDMKRATVLSGLFAGSGHTIIIDGYHKFFTNNLYSL
                     VPAPVIIDYIPERIVDYQKPTITGVQQYATINSDILRGLSDKKGTIIDIPNIFDHYMY
                     AFHFTDNYIASIKPHVDDYYFTIIDITHIAPEQLHSLGKALQKSFTHSMVAIYGNLMI
                     IGATDKQDGFAIDQAKMQTLENLIQTTEVSNLFINPEHALSYILSTSVQSVLPDDGEN
                     STVMLKFSKPDATIYPNSFIDTVINTNDAVTGLADKTPYWANTLQLRMFYAKRINQLL
                     KQSEYYGLKEQYEREMQALMELRRTGEYTPSLRQYIINVIAYKEKLYENIALMYEKDK
                     QWDKAISLYSALLAVNPNNFTANYRIGIISATIQNMDNAFKYLNNAMRMNPNNPDVLY
                     QLGILLVSQGKYTEALNYLLQAHQMNMATASLFFNIGLCYESLKNIEEAKRYYEKAKL
                     LDPNDPSIQSSLERFSGKTEEPVNPNADTRTNQLDEEKGESFPLPINQSAYDVRLKEN
                     EVKEQDKLAMPQ"
     gene            3405..3725
                     /locus_tag="PK348_01755"
     CDS             3405..3725
                     /locus_tag="PK348_01755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK64967.1"
                     /translation="MHSKNAFEYIPEDNLVVLFVLMVCTAGLYYFWWIARVSRIFYDN
                     PVTNILLTILTAGVWLIYLTIKYMQKSEMLNGRQMPWYMVLFLPIGFLIIQHNINERY
                     FTSQ"
     gene            3743..5452
                     /locus_tag="PK348_01760"
     CDS             3743..5452
                     /locus_tag="PK348_01760"
                     /inference="COORDINATES: protein motif:HMM:NF025691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4389 domain-containing protein"
                     /protein_id="HXK64968.1"
                     /translation="MIVRCSECNAAYQVDDEKVDNKRFAFTCPKCKAHVIIDNRIDIA
                     ADKSIDDMIMAHAVEEKPKKMAQAPEEFDTLLQEEPFEKEYTAEKISTTDEDLLADIE
                     SFDATQDKKDKSKDEFEPIESETIDLSDFSAEELKSATEEITLPEEPIEKDLLLDEEI
                     KADEIFSKEKDVDESITIDLDTLDIPLDESTVIEEAEMPGESSVLEKEELLVEDMGFD
                     DILVADEEIPQKPKAKKAPEKEPIDDITLDLDSLDIQLEEHAEVLPGEQIIEEDEKLT
                     LEDAGITIDELIEEEKKQSLEEEEDLKLSLDEIEPGLTVDDLHKELTESEIETLMSKE
                     IELPEVDLDKFELEEGEIKEFAPEEFEYAEKESYDSIPAGFVNLTIDYALSYSRPGAV
                     ARLLGLYLVTLIPHIIVSSIYSLVSSIVGFFNWCVVLFTGQFIDDFADMQQNTLRYYI
                     AILASAAQITEDRPVYAGRPKVEHSLQLDIVYPSEPSRLLAFLRITGIGILTAALPHL
                     LLLAVLTLGMLILIPVGLISIIATKRWPSLLFDFMYRYLRYYSRVNAYIMGLVDKYPS
                     FIF"
     gene            5510..>6300
                     /locus_tag="PK348_01765"
     CDS             5510..>6300
                     /locus_tag="PK348_01765"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HXK64969.1"
                     /translation="MDIHSIIHTVFSSGMLANYIHDFEYRAIQEQMATAIARSWYDEE
                     HIVIEAGTGTGKTFGYLLPAALFALLEDVKVAIVTETKALQQQLIVKDLPVVASILKN
                     ITDSSLEYALCLGSANYPCFRRLQHAIDTGSFDKKFAGEIANLQKLLKKNHTLTRFDV
                     QVPDALWNTICRESELCSMNKCRYKSTCSYMEARKQWQKAHILVMNHYLFFSNIAASK
                     SLVPDCKAVVFDEAHSLERIASGQLGFSINSQHLKETLEHMIQRK"
BASE COUNT         2019 a         1058 c         1294 g         1929 t
ORIGIN      
        1 cattccaaat aaaaacaatc ccaatccacc aaagattgtg atacctattg aaaatccatc
       61 catagtgtat aacctcgagt taataattta tattcatata caaatagtaa aacattattc
      121 tattgtggca tagcaagctt atcttgttct tttacttcat tttctttaag ccgcacatca
      181 tacgctgatt gattaatggg taatggaaaa ctttcgcctt tttcctcgtc aagctgattg
      241 gttcgggtat cagcgtttgg gtttacaggc tcttctgttt tacctgaaaa acgctctaaa
      301 cttgattgta tggaagggtc attggggtca agcagttttg ccttttcata gtagcgtttt
      361 gcttcttcaa tgtttttcag tgattcataa cacaggccaa tattgaagaa taatgatgct
      421 gttgccatat tcatctggtg ggcctgcagc aaataattca gcgcctcagt atatttgccc
      481 tgtgatacca gcagtattcc aagctgatac agaacatcag gattattagg gttcattcgc
      541 attgcattat taaggtattt aaatgcattg tccatattct gaatggttgc gcttataatg
      601 ccgatgcgat agtttgcagt aaaattattg ggatttacag caagcaatgc ggaatataat
      661 gaaatagctt tatcccattg cttgtccttt tcgtacatca gtgcaatatt ttcatagagt
      721 ttttccttgt atgcaattac attaataatg tattgccgta gtgaaggtgt atattcacct
      781 gtccgccgca attccatgag tgcctgcatc tcacgttcat attgttcctt taatccataa
      841 tattcagact gctttaaaag ctgattaatt cgctttgcat agaacatacg aagttgcagt
      901 gtattagccc agtatggagt tttatcggca agccctgtaa cggcatcatt ggtattaata
      961 acagtatcaa taaagctgtt tgggtatatt gtggcatctg gtttactaaa ctttagcatt
     1021 acagtactgt tttccccatc gtcaggtaat accgattgta cagatgtact aagtatatag
     1081 gataatgcat gctcaggatt gatgaaaagg tttgaaactt ctgttgtctg aatgaggttt
     1141 tccaatgttt gcattttggc ctgatcaata gcaaatccgt cctgtttatc tgttgcgcca
     1201 ataatcataa gatttccgta tatggcaacc attgaatgtg taaaagattt ttgcaaggct
     1261 ttgccaagac tatgtaattg ctctggtgct atatgggtta tatcaataat agtaaagtag
     1321 taatcatcaa catgtggttt aatgctggca atgtaattat cagtaaagtg aaaggcatac
     1381 atgtaatggt caaaaatgtt agggatatca ataatggtcc cttttttgtc tgataatcca
     1441 cggaggatat cgctgttgat ggtggcatac tgctggacac cagttattgt tggtttttgg
     1501 taatcaacaa tacgctcagg gatgtaatca ataataacag gggcaggtac caggctgtac
     1561 aggttgttgg taaagaattt atgatagcca tcaataatga tggtgtgtcc ggaacctgca
     1621 aaaaggcctg atagcaccgt tgcccgtttc atatcgcgtg caaagtgatc ggtaaccaca
     1681 catgcaggta tcccctgtat atatgctgta gcaggttgtg tgatatatga tgcattaaaa
     1741 tttgccattg ctacagtgtc atatccggtg cataatctat tgaccagtgt atttgaaaat
     1801 ggcagcctga aataaatatg ggtaataata attgcaggga taaataacaa acttacaaca
     1861 aaatagagta ttgatggtac agaacgtttt gtattttgta aaagatacat gccgggaaag
     1921 ataatcgtga taattgccat aaggtaaatg agtataaaaa accacaggta cgttaattct
     1981 atccataaaa atgcaattat tatgggaatg ggtataataa agaaagaaaa aagtagtata
     2041 gtatatcgtg actgatgatt gtttttcagg ataatctgtt taagggaagt accaaatgtt
     2101 agtcctgaaa aaatactgaa aggcagagcg gtcataatac tgatatagta aggaatatca
     2161 ttgctgtaaa ttataataat aaaacccagg aaaaggaatg gatagagcat ctcaccataa
     2221 ataaaaactg tgttgtgtgt ttttatatac tgtgaggcga tagttcctac gataaagcct
     2281 gccgcaatgg caattgctga ataaaaaata atttgcaggt atagtccata ccgggaaacg
     2341 atactcagca gtataaggat aataaatatg cttatgaatg agacattgca gtatgtgaac
     2401 agtatatcgt cccgtgacgt aattgcacgt tcttctgcaa caccacgtga aatgattgct
     2461 ggcgggcaaa aggggaggga aaggagtata gtgaacacac aggatagtat gggtaataca
     2521 acataaagaa taaggtagtg ttgattataa aatatgaaaa gcccaaacgc taaggatgca
     2581 ataaacgata gcagagcaaa acatactgct aaaatagcag gatttttatc atcgatataa
     2641 tcgccaatgg tagtctttaa gtaatatggg gcttttatac ctgtagaaaa atatagtaaa
     2701 aatatcgccc catatagcag ccagtaacaa tgttgaagga tagttccata tgcattgagg
     2761 atatcattgt acacaaaggc agctatgaca ggagctatcg ccacacatac tgcaataaat
     2821 tcctgtataa taaaaataat cctgtggttt tgtaatcgtt ttaaaatact gttgccagca
     2881 attataccag ccagtaaccc tgaaaatgca aacgttaaag tgtatttcca gacaggggaa
     2941 tacagtgtca gtgagcttac gtacaggata ttaagtaggg taaatactac cagggtggca
     3001 attgagaaaa ataccgaaag taacacaaat cgccgctggg ttatactgga atagttgaat
     3061 tcttttatgt tcattttttt gaatacgttc tcctgcatac tactgaattt attgttaacc
     3121 cctttaaggt tatggtatct caattcttga ttgatactat aatgacttga tgactaaaac
     3181 cgcagtcttt ttagcaaatt actgatacaa ttggcactgc tgaatgcatt tttatgacgt
     3241 agtatacgct atcgataaaa atgacaagaa tttttcttta gtaaataatt attaagattt
     3301 tttcatgatt ttaccgtaaa ttatatatag aagcttttgg actttttttg ttgcagtatt
     3361 ttattaattt tgatatatat gcaatagaat ttagtggagc gtatatgcac tcaaaaaatg
     3421 cgtttgaata tatacctgaa gacaatcttg ttgtattatt tgtattaatg gtatgtacag
     3481 cagggttata ttatttctgg tggattgccc gtgtaagccg cattttctat gacaatcccg
     3541 ttaccaacat actgcttacc attctcacag ctggcgtgtg gctcatttat ctaactatta
     3601 aatatatgca gaaatctgaa atgctcaatg gcaggcagat gccctggtat atggtattat
     3661 ttttacccat aggctttttg attattcagc acaatattaa tgagcggtat ttcaccagtc
     3721 aataactcat caggagccat tcatgattgt acgatgcagc gaatgtaatg cagcctatca
     3781 ggttgatgat gaaaaggtag ataacaagcg ttttgcattt acctgcccca aatgcaaggc
     3841 tcatgttatt attgataatc gtatagatat agcagcggat aaaagtattg atgatatgat
     3901 tatggctcac gcagtagaag aaaagccaaa aaagatggca caggctccag aagagtttga
     3961 tactctgctg caggaagaac catttgaaaa agaatatacc gcagaaaaaa tttcaacaac
     4021 tgatgaagat ttactggcag atattgaatc atttgatgca acgcaagata aaaaagacaa
     4081 aagtaaggat gaatttgagc ccattgaaag cgaaacaata gatttgtctg atttttctgc
     4141 tgaagaattg aaaagtgcta ctgaagaaat aaccttacct gaagaaccca tagaaaaaga
     4201 tttgttgctt gatgaagaaa ttaaggctga tgagattttt tcaaaggaaa aggatgttga
     4261 tgaaagtatc actatagatt tagataccct tgatatacct cttgatgaat caacagtaat
     4321 tgaagaagct gaaatgcctg gtgagtcgtc agttcttgaa aaagaagaac tactggtgga
     4381 agatatgggg tttgacgata ttttagtggc agatgaagaa atccctcaaa aaccaaaagc
     4441 aaaaaaagcg cctgaaaaag aaccaataga tgacatcacg ctggatctgg attcacttga
     4501 tatacaactg gaagagcatg cagaggtgtt gccaggtgaa cagataattg aagaagacga
     4561 aaagctaaca cttgaagatg caggaataac tattgatgaa cttatagaag aagaaaagaa
     4621 acaatcactt gaagaggaag aagacttgaa gctttcttta gatgaaattg agcccggtct
     4681 tacggttgat gacctgcata aggaacttac agaatcagaa atagaaacct taatgtcaaa
     4741 agaaattgaa ttacccgaag tggatctgga taaatttgaa ctggaagaag gtgagattaa
     4801 agaatttgca cctgaagagt ttgaatatgc tgaaaaggaa agttatgatt caatacctgc
     4861 aggttttgtc aatttaacca ttgattatgc attgtcatat tcacggcctg gtgcagttgc
     4921 acggctttta gggttatatt tagtcacgtt gatcccgcat attattgtca gttccatcta
     4981 tagtcttgta tcatctatag ttggattttt taactggtgt gtggtgttat ttaccggcca
     5041 gttcattgat gattttgcag acatgcagca gaacacatta cggtattata tcgcaattct
     5101 ggcatctgca gctcagataa ctgaagacag gcctgtttat gcaggcagac caaaagttga
     5161 gcacagcctg cagcttgata tagtatatcc ttccgagcca tcacgcctgc tagctttttt
     5221 gaggattacg ggtattggaa tactcacagc agcacttcct catctgctgt tgcttgctgt
     5281 gctgactctg ggaatgctta ttctgattcc tgttggcctt atcagtatca ttgcaacaaa
     5341 gcgctggcca tccctgttgt ttgatttcat gtaccggtat ctgcgctact attcccgggt
     5401 taatgcatat attatgggac tggttgacaa atacccttca tttatttttt gatagacatc
     5461 ccgcatagca ttcattacag tgccgctata aaaatatata tagcactcta tggatattca
     5521 ttctatcata catactgtgt tttccagcgg catgctggca aactatatcc atgattttga
     5581 gtatcgtgcc attcaggaac agatggcaac tgctattgcc cgctcatggt atgatgagga
     5641 acacattgtc attgaagcgg ggactggtac aggcaaaacg tttgggtatt tacttcctgc
     5701 tgccctgttt gcgctcttgg aagatgttaa agtggcgata gttactgaaa caaaggctct
     5761 gcagcagcaa ttgattgtaa aagatttgcc tgtggttgct tctatactaa aaaacataac
     5821 cgatagttcc ctggaatatg ccctgtgcct gggaagtgcc aattacccct gcttccgcag
     5881 gttgcaacat gcaattgata ctggaagttt tgataaaaaa tttgctggtg aaatagcaaa
     5941 tctgcaaaag cttttaaaga aaaatcacac tcttacacgc tttgacgttc aggtacctga
     6001 tgctctgtgg aatacaatct gccgtgaatc ggaattatgc tcaatgaata aatgcaggta
     6061 taaaagcacc tgctcatata tggaagcgcg aaaacagtgg cagaaggcac atattcttgt
     6121 catgaatcat tatctctttt tttcaaatat tgctgcgtca aagtcgctgg tgccggattg
     6181 taaggcagtg gtatttgacg aagcacattc tctggaaagg attgcgtcag gtcagttagg
     6241 cttttctata aatagccagc atcttaaaga aacactggaa cacatgatac aaagaaaaaa
//
LOCUS       DAOSZP010000045         6272 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_10537_length_6272_cov_3.425607, whole genome shotgun sequence.
ACCESSION   DAOSZP010000045 DAOSZP010000000
VERSION     DAOSZP010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6272
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10537_length_6272_cov_3.425607"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..379
                     /locus_tag="PK348_01770"
     CDS             <1..379
                     /locus_tag="PK348_01770"
                     /inference="COORDINATES: protein motif:HMM:NF012626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HXK64970.1"
                     /translation="VSLTLKGVEGIQKIESIMKHFRSNPPKQLAGTTVTAILDVKQGT
                     LTDVMSGTTKAVDLPASDVLQFTLENGSKVTMRPSGTEPKIKFYFSSRKKGTDIKQLE
                     EEVSRYNETLAKELLSTIESVIT"
     gene            complement(589..1032)
                     /gene="ybeY"
                     /locus_tag="PK348_01775"
     CDS             complement(589..1032)
                     /gene="ybeY"
                     /locus_tag="PK348_01775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HXK64971.1"
                     /translation="MAIKVNVEREHITMPKELAAFCKKIIAAVLQGQEVDNVVVTLIL
                     TNDEAIAHINKTYRKKKGPTDVISFAYREAPMPHVTTKEHLGDIFISLETAQRQAIEY
                     GVTLKEELKRLLVHGVLHLLGYDHEQSLYKKRKMQKKEEEIMQKI"
     gene            complement(1016..3253)
                     /locus_tag="PK348_01780"
     CDS             complement(1016..3253)
                     /locus_tag="PK348_01780"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HXK64972.1"
                     /translation="MVFKRGIQFAFKKKSLKASFIYTLIVIITATALLTLNRIGATYS
                     YQAGDIAREDIRAPMNIEYPVAIETEKEKNRIAEAMPAIFDLNESIVEDKLKISNMLC
                     EAVIKVKEQMPDSSPSKQVLLLRTMLPDYLQYSDRVLMGLLQYDEQLLHRDLNKILLQ
                     IYNNGILEKPYTNPLRLQSNHVIVRVVGSASELKEKQLNVDALVDMKKIQSLVPVISK
                     DILNKPSSDALYAVNSVIISLLQPNLFYNEEETKRRIAEAVQSVKPVTRVIKKGQSIA
                     REGDTITEDMLQKINIINKNAHFSGSSFIIGLLLIQLGFLVIFGYFLITYYSNLVVDN
                     KMPLIVFTLVLAFIVIAFFIARWQGRAFEGLFFPLLLPIPLVTMVLAIMYNMHIAMLI
                     GVYIVFFVTVSMKGDFTTLLLAFSSALSGVFVVGDVERRSDFLRGGVTLGLINSVIVI
                     AVGLMQEMQFGIIANNVGLAFISGIINAIIVFGVFPVYENLFGVTTKFKLLELSDLNA
                     PIFKEMLIKAPGTYNHSLMVATLAESACKEVGANALLARVGGYYHDIGKIQDANFYIE
                     NKVTDPRAKDMSALEYCRLIISHVEKGVELARKNNLPEMVIDFIREHHGQTVMTYFYH
                     QALEEGTTNGNSIQKSDFQYPGPKPHTRETAIVMLADAIEAASRSIQEPTLEKLETLV
                     KRIIYNRLNEGDLERADITLNELNKIQNAFVQVLQGIFHSRIEYPTKEAINRLEKKSS
                     NGNQG"
     gene            complement(3287..4246)
                     /locus_tag="PK348_01785"
     CDS             complement(3287..4246)
                     /locus_tag="PK348_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HXK64973.1"
                     /translation="MVDTKFEIDDISIYKRICGYKDKNIKAIEKLLDVKIIPRGNTLI
                     VEASGDNAAKALQLLHLMGDYLYMRDSLYEFDDFDIRYLALSINQGIKVDADEINKLK
                     IVIPETGKTIVPKTINQANYMSAIHKSPITISIGPAGTGKTYLAVAVGVKFLLTGRVE
                     RIILTRPAVEAGESLGYLPGDFIQKINPYLRPLYDALFDILPYERISKYMEKGIIEIA
                     PLAYMRGRTLNKAFIILDEAQNTTSSQMKMFLTRLGNSSKMVISGDITQIDLEKPRQS
                     GLLAAQKILHGIEEITFIEFSKEDICRHPIVEKIIAAYEKHAE"
     gene            complement(4246..6021)
                     /gene="aspS"
                     /locus_tag="PK348_01790"
     CDS             complement(4246..6021)
                     /gene="aspS"
                     /locus_tag="PK348_01790"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011916245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HXK64974.1"
                     /translation="MFEQRNYCGELTVNDVGTMVRLAGWVDTKRDLGGIIFIEMRDVT
                     GIVQVVFDASRNSTAYENADKVKNEYCIFVTGQVAKRSDETVNPNLKTGMIEVIGQDI
                     SILSESEVTPFPIDTYTNLNEEVRLKYRYLDLRRADMQEAIIVRHKVMQTIRRYLTDN
                     RFIEVETPILNKSTPEGARDFLVPSRINRGMFYALPQSPQLFKQILMISGFDRYFQIA
                     KCFRDEDLRNDRQPEFTQVDLEMSFVNPQMVMQIIEGLLKEIVREVLDKEIETPFPVF
                     TYDEVMQRYGKDAPDTRFGLELNDVSDIFTNSTFNVFKAALENGGIIKAIVINDEGKI
                     SRAMIDEYINYVRTFKAMGLPSVRYTGSAFETGIAKFLSDSEKQKLVDRLKLNSNALI
                     IFSCDKPKIVNDALGNLRIKIAQQLNLIDENKLNFLWVVDFPLLEYDAEEGRYYAMHH
                     PFTAPKPECEPMLDTVTPQTTQDIKAQAYDVVLNGVEIGGGSIRINRMPLQKKMFGIL
                     GISEEEAKIKFSFLLDALKYGAPPHGGIALGLDRILMLLLKRNSIRDVIAFPKTQKGQ
                     CLMSEAPSPVSAQQLKELSIKVVEL"
BASE COUNT         1858 a         1355 c         1111 g         1948 t
ORIGIN      
        1 tgtttcgctt acactgaagg gtgtggaagg cattcaaaag atagaatcca tcatgaagca
       61 ctttagaagt aatccaccca aacaactggc cggaactact gttactgcaa ttcttgacgt
      121 caaacaggga acgcttactg atgtaatgag tggtaccaca aaagctgtgg atttgcctgc
      181 atcggacgtg ttgcagttta cgttagaaaa tggctcaaag gtaaccatgc gtccttcggg
      241 aactgaaccc aaaatcaagt tttatttcag ctcacgtaaa aagggaacag acatcaaaca
      301 acttgaagaa gaagtatcac ggtataatga aacgttagca aaagaattat tgagtacaat
      361 tgaatcggtt attacataac tacaaagcat ttatactatg ctttaatcta caaatttttg
      421 ttgccatatc cacaaccaca agatctatga atttacggtt tagtggcact gaacatcttg
      481 tatgggcaaa gccactgcac tttattaaag tgtagcaatc tcatcctcac aaaagagatt
      541 gcctgacttt gcttgcaatg attatggtga gttaggcagg agtgctcatc aaatcttttg
      601 cataatctct tcttcttttt tctgcatttt tctttttttg tataacgatt gttcatggtc
      661 atatcccaac aggtgcagca ctccatgtac cagtaatctc tttaattctt ctttcagggt
      721 cactccatat tctatagcct gcctttgggc agtctccagt gaaatgaaaa tatcgcctaa
      781 atgttccttt gtggtaacat gtggcatggg tgcttcacgg taggcaaaag atatgacatc
      841 ggttggacct ttttttttgc gataggtttt attaatatga gctatcgcct catcattagt
      901 taaaatgagt gtaacaacta cattatcaac ttcctgaccc tgtaaaacag cagctataat
      961 ttttttgcaa aacgcggcta actctttagg catcgtgatg tgctcacgtt caacattaac
     1021 cttgattgcc attagatgat tttttctcca atcggtttat tgcttccttt gtggggtatt
     1081 caatacgtga atggaatatg ccctgtaaca cctgcacaaa cgcattctga attttgttta
     1141 attcattcag cgtaatatcg gccctttcca ggtcaccttc attcagccta ttatagatta
     1201 ttcgttttac cagtgtttcc aatttttcca gtgttggctc ctgtattgaa cgcgatgcag
     1261 cttcaattgc atccgccagc atgacaattg cggtttcacg tgtatgtggt tttgggccag
     1321 gatactgaaa atcacttttc tgtatgctat tcccgttagt ggtgccttct tcaagtgcct
     1381 gatggtaaaa atatgtcatc actgtttgtc cgtgatgctc acggataaaa tcaatgacca
     1441 tttcaggaag attatttttt cgtgccagtt caacgccctt ttcaacatgt gagataatta
     1501 atctacaata ttcaagggct gacatatcct tagcacgtgg gtcggtaacc ttattctcaa
     1561 tataaaaatt tgcgtcctga atttttccta tatcatggta gtacccgcca acacgtgcca
     1621 gcaatgcatt agcaccaacc tccttgcatg cagattctgc aagtgttgcc accatcagtg
     1681 aatgattata ggttccgggt gccttgataa gcatctcttt aaatattggc gcattgaggt
     1741 ccgatagttc cagcaactta aactttgttg taacaccaaa gaggttttca tataccggga
     1801 acacaccaaa gacaattatt gcattaatga tacctgatat aaaagccaat ccaacgttat
     1861 ttgcgatgat gccaaattgc atttcctgca tcaatccaac agcaattact attaccgaat
     1921 taataagccc taaggtaaca ccaccacgta aaaaatcaga acgacgttca acatcaccaa
     1981 caacgaatac acctgataat gcggaactga atgccaatag taatgtggta aagtcgcctt
     2041 tcatgcttac cgttacaaag aacacaatat acacaccaat caacatggca atgtgcatat
     2101 tgtacatgat tgcaagtacc atcgtgacca ggggaatagg taaaagaagt gggaagaaca
     2161 aaccctcaaa agcccttccc tgccacctgg ctataaaaaa ggcaataaca ataaaagcca
     2221 gaacaagggt aaacactatc agaggcatct tattatcaac aaccaggttg ctataatagg
     2281 ttatgagaaa atagccaaag atgactaaaa atcccaactg aataagaagc agccctataa
     2341 taaaacttga tccagagaag tgcgcattct tattaataat attaattttt tgcaacatat
     2401 cctctgttat ggtatcacct tcacgggcta ttgactggcc tttctttatc acccgggtaa
     2461 ctggttttac cgactggaca gcttcagcta ttcttctttt tgtttcttcc tcattataaa
     2521 aaagatttgg ctgcaacaga ctgataataa cagaatttac tgcatataac gcatcactgg
     2581 aaggtttgtt aagaatatct ttgcttatga ctggcaccag cgattgtatt tttttcatat
     2641 ctactaatgc atcaacattc aactgttttt cttttagttc tgaagcactt cccactactc
     2701 tcacaatgac atgatttgac tgtaaccgta atggattggt atatggcttt tccagaatac
     2761 cattattata tatttgtaat aaaattttat tcaggtcccg gtgaagaagc tgttcatcat
     2821 attgcaacaa tcccattaat accctatccg aatactgcag ataatcaggc aacattgtcc
     2881 tcagcaacaa cacctgcttt gaagggctac tatcgggcat ttgttcttta actttaatta
     2941 ctgcctcaca cagcatgttg cttattttaa gcttatcctc tacaatggat tcattgaggt
     3001 caaagattgc aggcattgct tcggcaattc tattcttttc tttttcagtt tctatagcta
     3061 cgggatattc aatgttcatg ggtgctctaa tatcctcacg ggctatatcc ccagcctgat
     3121 aactgtatgt agcacctatc ctgtttaatg taagaagggc agtagcggta ataataacta
     3181 tcaatgtata aataaaagaa gctttcaggc ttttcttctt gaaagcaaac tgtatgcccc
     3241 ttttaaaaac cataaatgaa tttaacatat cagtaaccgg attgtattat tcagcatgtt
     3301 tttcatacgc tgcaataatt ttttcaacta ttggatgccg gcagatgtcc tcttttgaga
     3361 attcaatgaa ggtaatctct tcaatgccat gaagaatctt ttgtgcagca agtaaaccag
     3421 attgacgtgg cttttccaga tctatctgag taatatcacc agatataacc atttttgaac
     3481 tgttacccag acgtgttaaa aacatcttca tctgtgatga agtggtattc tgtgcttcat
     3541 caagtataat gaatgcctta ttgagtgtac gtccgcgcat ataggctagc ggtgcaatct
     3601 ctataatccc tttttccata tacttggaaa ttctttcata gggaagaata tcaaacaggg
     3661 catcatacag cggccttagg tatggattga tcttctgaat aaaatcaccg ggtaagtacc
     3721 ctaaactttc acctgcttca acagcaggcc gtgttaaaat aatgcgctcc accctgcccg
     3781 ttaacaggaa ctttacacct acagctactg ccagatatgt ttttcctgtc ccggccggcc
     3841 caatggagat agtgatgggg cttttatgga ttgccgacat gtaatttgcc tgattgattg
     3901 tcttgggtac aatggttttc cccgtttcag ggataactat ctttagtttg ttgatttcat
     3961 cagcatctac tttaatcccc tgattaatag aaagtgccag gtagcgtata tcaaaatcat
     4021 caaactcata gagggaatca cgcatatata aataatcacc catcaggtgc aaaagctgta
     4081 aagctttagc ggcattatca ccggaagctt caactatcag ggtattgcca cgggggatta
     4141 tcttgacatc aagaagcttt tcaattgctt tgatattctt atctttatac ccacatatgc
     4201 gtttgtaaat agatatatca tcaatttcaa attttgtatc taccattata attcaaccac
     4261 cttgatagat agctctttta actgctgtgc actcacaggc gaaggcgctt cactcatgag
     4321 gcactgtcct ttttgcgttt tggggaatgc aatcacatca cgtattgaat tgcgtttgag
     4381 taacagcatc aggatcctgt ccaaccccag tgctattcca ccatgaggcg gtgcaccata
     4441 ttttaatgca tccagcaaaa atgaaaactt tatttttgcc tcttcctcgg aaataccaag
     4501 gatgccaaac atctttttct gtaatggcat gcggttaatt cggatagaac caccgccaat
     4561 ttcaacacca ttcagtacaa catcatatgc ctgcgccttg atgtcttgag ttgtttgcgg
     4621 agtaacagta tcaagcattg gctcgcattc aggttttggc gctgtaaacg gatgatgcat
     4681 tgcatagtag cggccttcct cagcatcata ttccagaaga ggaaaatcaa ccacccacag
     4741 gaagttgagt ttattttcgt caattaaatt aagctgctgt gcaatcttga tacgaagatt
     4801 gcccagtgca tcattgacaa tctttggctt atcacaggaa aaaataatga gtgcattgga
     4861 attgagtttt aacctgtcaa caagcttttg cttttcacta tcgcttaaaa attttgctat
     4921 ccctgtttca aaagcactcc ccgtataacg cacacttggc agccccattg ctttaaacgt
     4981 gcgcacataa ttaatgtatt catcaatcat tgcccgtgaa attttgcctt catcgttaat
     5041 aactatcgcc ttaattatgc caccattttc cagtgctgcc ttaaaaacat taaacgttga
     5101 attggtaaag atatcactta catcattcag ctcaagtcca aaacgtgtgt caggggcatc
     5161 tttgccatag cgctgcataa cttcatcata cgtgaataca gggaacggcg tttcaatttc
     5221 tttatccagc acctctctaa ctatctcttt caaaagccct tcaataattt gcataaccat
     5281 ttgaggatta acaaatgaca tttctaagtc aacctgagta aattcaggct ggcggtcatt
     5341 gcgcaggtct tcatcacgga agcattttgc aatctgaaag tagcggtcaa aaccggaaat
     5401 catcaaaatt tgtttgaaaa gctgtggcga ttgcggcagt gcataaaaca taccacggtt
     5461 aatacggctt ggaacaagaa aatcacgcgc accttcagga gtggacttat tcagtatagg
     5521 cgtttcaact tcaataaacc ggttatcagt aagatatcgc cgtattgtct gcataacctt
     5581 atgacgtact ataatagctt cctgcatatc agccctgcga agatctaaat accggtactt
     5641 caaccgcacc tcttcgttca ggttagtata ggtatcaatg ggaaatggtg tcacctcaga
     5701 ctctgataaa atggaaatat cctgcccaat aacctctatc atgcctgttt tcaaattggg
     5761 attgactgtt tcatcagaac gctttgcaac ctggccggtt acaaaaatac agtattcatt
     5821 tttcacttta tcagcatttt catacgcggt actattcctg gaggcatcaa acacaacctg
     5881 tacaattcca gtcacgtccc tcatttcaat aaatattatg ccacctaaat ctcgcttggt
     5941 atcaacccag ccagccaaac gaaccattgt accaacatca ttaactgtta actcaccaca
     6001 gtagtttctt tgttcaaaca ttcctctaac ccaatagaaa ataatatagc aacataccag
     6061 aatcaattaa aaattcaaaa aaatatggga attttgctcc ccgcctgaag cggtttcccg
     6121 ccaacaggac acagtagcaa ataatttgat ttcgatagta ttacggaggc aggctgattt
     6181 ttgtcaagta attatatgca catccattat ttcattgttt tgaacgtgtt tacaatggct
     6241 tcacactgtt tttttgactt cccttcagca at
//
LOCUS       DAOSZP010000046         6265 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_10555_length_6265_cov_3.398229, whole genome shotgun sequence.
ACCESSION   DAOSZP010000046 DAOSZP010000000
VERSION     DAOSZP010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6265
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10555_length_6265_cov_3.398229"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(72..1913)
                     /locus_tag="PK348_01795"
     CDS             complement(72..1913)
                     /locus_tag="PK348_01795"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HXK64975.1"
                     /translation="MKVELHSYNSKIKNFTLQFILRTEGISYFIIVPILLLFLSINIT
                     FTYNQLIFFIICIAFAFPISFITTQINNIIVTKPVQKYFNAVVKNEAIPEESYELAFK
                     RFLSLPYLHSIGAFFRWVFGLSMFTVPITLSQQFSATQVFISWMSILICAPSGTVLYF
                     LLTEIFIQEIYNTGIFPKIPENFTFKNTMGITKKLMISITIIVLTPFFIMIAFIATIL
                     DQGAIHLSSSWWKLFIFGSIGLLFSLFLAKLLAKTLIMKTNIIKEFLFAVGKGQLAAY
                     TKKIAVSDELAEINIAVYNMKENLRRMVELIRSSSLELKATSNNMKVSSTKFSEASRD
                     LTAIIEETSSAYEEMSSSFEMIVNTIKLQLEQANTVNTEITVINSSGANLTHQVNELT
                     GTFNEAINGVEKGKETIHRSVSAITDISQYLETVESTISTINEVADQINLLALNAAIE
                     AARAGEHGRGFAVVADEVNKLADQTASLVKSIQSTIEQYSSKIKNEIKFINETAALFE
                     SVRDKMLATQNVLNITHQFTKELTTQNESIMGKIEKLVQLSNEIYNTSNEQQITIDEL
                     TKAINSINEVATHTAEESFTIQSLSEKLEDNARRLLENIEYFKISYE"
     gene            complement(1976..3226)
                     /locus_tag="PK348_01800"
     CDS             complement(1976..3226)
                     /locus_tag="PK348_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015949203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HXK64976.1"
                     /translation="MQLPAYYEFCNSVKTIVGHKALEQIPQSLKAINAQRPMIITDKG
                     VEQAGLIKIVQAAMQKSKLKAVALFDSVPADSDVKVVNEVAKIYRAKKCDSIIAVGGG
                     SVLDTAKGANILVSENAADLMQFVGYGNVKRKLRPFVAIPTTAGTGSEVTSVAVIANH
                     EKNIKMAFGSLFLLPDIAIIDSRMTKTLPPTITAFTGMDALTHAVEAYFCLAKNPLSD
                     TTALAAIQLIAQNLVNVVKKPSDLDGRLALALASNLAGMAFSNSMVGLVHNIGHAIGG
                     VCGVPHGVCMNILLPYGLEYNMHKRSQYIGELLYPLAGEEVYNKTPKKERPLKTVEYI
                     RKLIQDLHDATDGKHPRFLKEVYDRTGKQMIPKEKLPDIARTAMSDPAQFYNPEDLDY
                     NDYIMVLEAAWEGIPLDRKKIKKG"
     gene            complement(3249..3791)
                     /locus_tag="PK348_01805"
     CDS             complement(3249..3791)
                     /locus_tag="PK348_01805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK64977.1"
                     /translation="MNALQYPEIKPKKKFIKRIYIAVMLWVVGRAIQAAAKVDREVKE
                     EFAKLKDNFLLKMWIWPNGPAMYVGKTKDGKVKYLGWKDKGYKPTLTMTIKSVEAAML
                     LFTFQEGTAISTARNRLVVDGDLPDACALVRILNIVEVYLLPKIIAKLAVKRYPKWSQ
                     MNPLRKHFGRILIYVRAFTF"
     gene            complement(3814..4956)
                     /locus_tag="PK348_01810"
     CDS             complement(3814..4956)
                     /locus_tag="PK348_01810"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HXK64978.1"
                     /translation="MIPYNFTFLSHPKICSGLKALEHIPFELESLNARKPLVITSARW
                     AKKGLAKTLTKALYDSNITIGALFTNAPNYPSVTIINELSKLYRDRGCDSIIALGSGG
                     IAHIAKAVNMVVSLKTDILQCTDLTISSPLKPLICVPTADCEGGEMAAVAYYERFEKQ
                     SDFLYPDVIVIDSRITRHATSQEIANCALTSLAHAIEACGIVAPNPINDAYAHTALSF
                     IYQYFPKTIKKPCYKDGSVAIVNAAACADTAFSNAPQGIVHTLAQAIALDTNLQPGIV
                     MGILLRYSLAAKLHAKGGIRPELLLALAGIDTYAKTPEKDRQKAAYDYLTKCIDATCN
                     YVPSTLKGLHIPHYKLKEYARLAAAKNKQHKENEYMALLEKSFEGK"
     gene            complement(4969..>6265)
                     /locus_tag="PK348_01815"
     CDS             complement(4969..>6265)
                     /locus_tag="PK348_01815"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HXK64979.1"
                     /translation="VLGSKNVKAIVAKGNEFKIVPKNPRKFDKARKKTLKFINRNPIT
                     SVSYRQFGTNANVNLNNAGGLLPVRNFTGGSHGEAHKISGEEMAERFDTKFSTCKPCA
                     ILCGHKGNINGEIRQVPEYETIGTFGSNLEIFDPVAVSEFNELCTKYGLDTISTAVTI
                     AWAMEATEKGLIKTDLKFGSPDNVAKTITDIAYRRGFGNDLANGTRKLSQKYGGADFA
                     IHVKGLEMAAYDPRGAWGQALSYAVANRGACHLSTASFAVEVYLNMHDPFSLKGKGSM
                     IAFLEDVTCAINSMHVCQFTAFAVELEPFVVKYTPTFLLNPMVTYLNPIATKFMDVSL
                     WPEMWSGITGKRMGMFKWIKAGRRIHVLERYMNTREGISRKDDTLPQRFLTEGRHCDP
                     QQRTVPLEELVDSYYKARGFDENGIPKDRTLKRLKIEKK"
BASE COUNT         1758 a         1194 c         1187 g         2126 t
ORIGIN      
        1 atttttgaat aaaatgtaaa gtagtaaata gtcccacctt aaaaggctat gtactgtctg
       61 caaataatta attattcgta ggaaatttta aaatattcta tattttccag tagtcgcctg
      121 gcattatctt caagtttttc tgataaggat tgtatagtaa aagattcttc tgcagtatgg
      181 gtggctactt cattaataga attaattgct ttggtcagtt catcaattgt tatttgctgc
      241 tcattggaag tattgtatat ctcattggaa agctgtacta acttttcaat tttccccata
      301 atgctttcat tttgtgtggt tagttctttt gtgaattgat gggttatatt caatacattt
      361 tgcgttgcaa gcattttatc acgaactgat tcaaataatg cagctgtttc atttataaat
      421 tttatttcat ttttaatttt actggagtac tgttctatgg ttgattgaat actcttgact
      481 aaagatgctg tctgatcagc caatttattc acttcatctg caacaacagc aaaccccctt
      541 ccatgttccc cagcacgtgc ggcttcaata gctgcgttga gtgccagaag gtttatctga
      601 tctgccactt cattaattgt tgatattgtt gattcaactg tttccaggta ttgagaaatg
      661 tcagttatag cgcttactga cctgtgtatt gtttccttac ctttttccac tccattgatg
      721 gcttcattaa aagttcccgt taattcattt acctgatgtg ttaagtttgc cccagaagaa
      781 ttaataacgg ttatttcggt atttaccgta tttgcctgtt caagttgtaa tttaatagta
      841 tttactatca tttcaaatga tgatgacatc tcttcatatg ccgagcttgt ttcttcaata
      901 atagcagtga ggtcgcgcga tgcttctgaa aactttgttg aagaaacttt catattattg
      961 cttgttgctt taagttctaa agaacttgag cgaataagct caaccattcg tctaagattt
     1021 tctttcatat tataaacggc tatattaatt tccgccagtt catcagaaac ggctatcttt
     1081 tttgtatatg cagctaactg ccctttccca acagcaaaca gaaattcttt aataatgttg
     1141 gtcttcataa taagtgtttt tgctaacaat tttgcaagaa ataaagaaaa tagtaatcct
     1201 atgcttccaa agatgaaaag tttccaccat gaagaagaaa gatgtatagc tccttgatcc
     1261 agtatggtag ctataaatgc tatcattata aagaatggag taaggacaat aatggtaatt
     1321 gatatcatta gtttttttgt aatacccatt gtgtttttga atgtaaaatt ttccgggatt
     1381 tttggaaata tacccgtatt ataaatttcc tggataaata tttcagtaag gaggaaatac
     1441 aatacagttc ctgacggagc acatattaat atactcatcc agcttataaa gacctgtgtg
     1501 gctgagaatt gttgtgacag tgttatggga acggtaaaca tcgataatcc aaaaacccat
     1561 ctgaaaaaag cgccaatgct gtgcaaatag ggtagtgata gaaatctttt aaatgccaat
     1621 tcatatgatt cttctggtat ggcttcattt tttactactg cattaaaata cttctgtact
     1681 ggctttgtca caataatgtt gtttatttga gtagtaatga atgaaattgg aaatgcaaaa
     1741 gcaatacata taataaagaa tataagttga ttgtatgtaa aagtaatatt tattgataaa
     1801 aaaagtaata gtatgggaac tataataaag tagcttattc cttcagtgcg gaggataaat
     1861 tgcaatgtaa aatttttaat ttttgaatta tatgaatgga gttcaacctt catacataat
     1921 cctcataaag tacttataac agtgccatga ataattcacg gcactgtttt ttacattatc
     1981 ctttttttat ttttttcctg tcaagcggta ttccttccca tgctgcttct aaaaccatga
     2041 tgtaatcatt ataatccaag tcttcgggat tataaaattg tgcggggtcg ctcattgctg
     2101 ttctggcaat atcaggtaat ttttctttgg gtatcatctg cttgccagtt ctgtcatata
     2161 cctctttcag aaagcgtggg tgtttgccat cagtggcatc gtgcagatcc tgaataagtt
     2221 ttcgtatata ttctacagtt ttaaggggtc gttctttctt tggtgttttg ttgtatactt
     2281 cctcaccagc aagtggatag agtaactcgc caatgtactg tgaacgtttg tgcatgttgt
     2341 actccaatcc atacggcagg agtatattca tgcatacacc atgcggtaca ccacacacgc
     2401 caccaatagc atgcccaata ttgtggacaa ggccaaccat tgagttggaa aatgccatgc
     2461 cagctaagtt tgaagccagt gccagtgcta acctgccatc aagatcagaa ggttttttaa
     2521 ccacattgac taaattctgt gcaataagct gtattgctgc aagcgctgtt gtgtcgctta
     2581 atggattttt tgccaggcag aagtatgctt ccactgcatg ggtaagggca tccattccag
     2641 tgaaagctgt tatggttggt ggcaacgttt tggtcatgcg ggaatcaata attgcgatgt
     2701 caggcagaag aaaaagagat ccaaaagcca ttttgatgtt cttttcatgg ttggcaatga
     2761 cagcaactga agtcacttcg gaacctgtac ctgctgtagt tgggattgct acaaatgggc
     2821 ggagtttgcg tttaacattg ccataaccta caaattgcat taaatcagct gcattttcag
     2881 atacaagaat atttgcccct ttagcagtat ccagaacaga gccaccacct acggcaataa
     2941 ttgaatcaca tttttttgcc ctataaattt ttgccacttc attgactact tttacatctg
     3001 agtctgccgg aacactgtca aagagtgcaa cggcttttag ctttgatttt tgcattgctg
     3061 cctggacaat ttttatgagt ccggcctgct caacaccttt atcagtaata atcattggtc
     3121 tttgtgcatt gattgctttc aagctctggg gtatttgctc cagggccttg tgaccaacta
     3181 ttgttttaac actgttacaa aattcataat atgctggtaa ttgcatgatt gcacctcgtt
     3241 tcatttattt agaatgtaaa tgcacgaaca tagataagta ttctgccaaa atgttttctt
     3301 aaggggttca tctgtgacca tttggggtat cgtttaactg caagctttgc aataattttt
     3361 ggcagtaaat aaacttctac tatattcaga atcctcacca gtgcacaggc atcaggcaaa
     3421 tccccatcta caaccagcct gttgcgagca gttgagatag cagttccttc ctggaatgta
     3481 aaaagaagca ttgcagcctc aacacttttt atggtcatgg ttaatgtggg tttgtagcct
     3541 ttatctttcc agcccaggta ttttaccttg ccatctttag ttttgccaac atacattgca
     3601 ggtccatttg gccatatcca catcttcaat aaaaaattat cttttagttt tgcaaattct
     3661 tcttttactt ccctgtcaac ttttgctgca gcctgaatgg ctcggccaac cacccacagc
     3721 ataacagcaa tatatattcg tttaatgaat ttcttctttg gcttaatttc aggatattgt
     3781 aatgcgttca taatgtaact cctttttaaa aatttatttg ccttcaaagg atttttctaa
     3841 cagtgccata tattcatttt ccttgtgctg tttgttcttt gcagcagcta atctggcata
     3901 ttctttcagt ttgtaatggg gaatatgtaa tcccttcaat gttgacggaa catagttaca
     3961 tgttgcatca atacactttg tgagatagtc ataggcggct ttctggcggt ccttttcggg
     4021 agtctttgca tatgtatcaa tgcctgcaag agctaacaat agttcagggc gtataccacc
     4081 tttagcatgc agtttggcag ctaaactgta tctgagcaga attcccatca caatgccagg
     4141 ctgaagattg gtatcaagag ctatcgcctg cgctaatgta tgaactattc cctgtggtgc
     4201 atttgaaaat gcagtgtcag cacaggctgc cgcattaact atggcaacac tgccatcttt
     4261 gtaacagggc tttttaattg tttttggaaa gtattgataa ataaatgaaa gtgctgtatg
     4321 tgcatatgca tcattgatag gattgggtgc aacaatgcca cacgcctcaa ttgcatgtgc
     4381 cagtgatgtg agtgcacagt ttgctatttc ttgtgaagtt gcatggcgtg tgatgcgcga
     4441 gtcaatcaca ataacatcgg gatagagaaa gtccgattgt ttttcaaaac gctcataata
     4501 tgcaacagca gccatttcgc caccttcaca atctgcggtg ggtacgcaga tcaatggctt
     4561 cagtggtgat gaaatagtaa ggtcagtaca ttgcaaaata tcagttttaa gggaaacaac
     4621 catattgact gcttttgcaa tatgtgctat tccacctgac ccaagagcta tgattgaatc
     4681 acaaccccga tctctgtaca gtttcgataa ctcattgatt atagttacac tgggatagtt
     4741 aggtgcattg gtaaaaagag cacctattgt tatgttacta tcgtataaag cttttgttaa
     4801 tgtcttagct aaaccttttt ttgcccatcg ggctgatgtt attacaaggg gcttgcgggc
     4861 attcagtgat tctaattcaa atgggatatg ttccagcgct ttaagccctg aacagatttt
     4921 tggatgactt aaaaatgtaa aattgtatgg aatcatggtg tcacctcatt attttttttc
     4981 aatttttaat cgttttaatg ttctgtcttt aggaatgcca ttctcatcaa agccacgtgc
     5041 tttatagtaa ctatctacca gttcttccag tggtactgta cgctgctgtg gatcacagtg
     5101 cctgccttcg gttaagaatc gttgtggaag agtatcatct ttccgtgaaa tgccttcgcg
     5161 ggtattcatg tatcgctcaa gaacatgtat ccttcttcct gctttaatcc acttgaacat
     5221 acccattcgt ttgccggtaa tcccggacca catttctggc cacaggctta catccatgaa
     5281 ttttgtggca attgggttaa gataggttac cattgggtta aggagaaacg ttggagtgta
     5341 ttttaccaca aagggttcca gttcaactgc aaaggcagta aactggcata catgcatgga
     5401 attaattgcg caggtaacat cttccaaaaa tgcaatcatg gaacctttac ctttaagaga
     5461 gaaaggatca tgcatattca gatatacttc aacagcaaag cttgcagtag aaagatggca
     5521 cgcaccacgg ttagcaacag catagcttaa tgcctgcccc cacgcaccac ggggatcgta
     5581 ggctgccatt tcaaggcctt taacatgtat tgcaaaatca gcaccaccat atttttgcga
     5641 tagtttccgg gtaccattgg ctaaatcatt accaaatcct cttctatatg caatatcagt
     5701 aatggttttt gctacattat caggtgatcc aaatttgagg tctgttttaa ttagtccctt
     5761 ttcggtagct tccattgccc atgctatagt aacagcagtt gatattgtat caaggccata
     5821 ttttgtacac aattcgttaa attcgctgac agcgactgga tcaaagattt ctaaattgga
     5881 accaaacgtc ccaatagttt catattcagg aacttgacgt atttcaccat ttatgttgcc
     5941 tttgtgacca cacaaaatag cacagggctt gcacgttgaa aatttggtat caaagcgttc
     6001 agccatttct tcaccggaaa ttttgtgtgc ttcgccgtgt gatccaccgg tgaaattgcg
     6061 aaccggcaat aaccctcctg cattattcag gttaacattt gcattggtac caaactgtcg
     6121 gtatgacacg gatgtaatgg gattgcgatt gataaatttg agggtttttt tacgagcttt
     6181 atcaaacttt ctgggatttt tgggtacaat cttgaattca tttcccttag caactatcgc
     6241 tttaacattt tttgaaccca gtact
//
LOCUS       DAOSZP010000047         6214 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_10653_length_6214_cov_3.153596, whole genome shotgun sequence.
ACCESSION   DAOSZP010000047 DAOSZP010000000
VERSION     DAOSZP010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6214
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10653_length_6214_cov_3.153596"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1073)
                     /locus_tag="PK348_01820"
     CDS             complement(<1..1073)
                     /locus_tag="PK348_01820"
                     /inference="COORDINATES: protein motif:HMM:NF024873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HXK64980.1"
                     /translation="MNLKDKLNIYYTDSGKPVTDSSWIQDAGGEIITTPFGQTITFVQ
                     QYPVASLHIDDTGTDIAAYCAMHRMEPITLDQCVFFDIETTSLSSGTGNYAFLIGLGY
                     FSENKFTVEQVFMRQYGEEPAVLFHCAERLQKFKTIVTFNGKTFDIPIIKTRYRINRI
                     QGFPVSMPVIDLLKPARSIFKSIYASCSLKSLEELLLGITRTDDIPGYQIPDVYFTYQ
                     QTGYDPRLTAVIEHNRIDITSMVLLLVFFNSLYTLIHTKQFDQVPTHLYKNIAKHLYS
                     RNIQLFIELIEYAGYALLDNDHSLFKKFSTALKRSGLLEQALTFWEKHINLFSLEELA
                     KHYEHRVKDYHKALSYCTWATAH"
     gene            complement(1036..3315)
                     /locus_tag="PK348_01825"
     CDS             complement(1036..3315)
                     /locus_tag="PK348_01825"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013916657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HXK64981.1"
                     /translation="MTIEQLIEYLKNYEPFMKQVAAWYTVPARQAVYAPYPDDIDERI
                     RNVYMQRGIENLYSHQRSAYDAVQKKENIVVVTPTASGKTLCYNLPVLNFMAKHPDAR
                     ALYLFPTKALSQDQLSELQEIMKITGLSIKTYTFDGDTPQSARKAIRTAGNIVITNPD
                     MLHSGILPHHTIWVKLFENLQYVVVDEIHTYKGVFGSHFANLMRRLKRICSFYGSHPQ
                     FICCSATIANPKELAEKLFEMPFTVIDNNGAPCGKKHFVIYNPPVVDKALGIRASAVK
                     EAAKVGTLCVKNNISTIVFCRSRLRVEIIATYLKEQCKGFSVAAYRGGYLPNERRSIE
                     KGLREKKINAVVSTNALELGIDIGMLDCAISVGYPGSIASLQQQSGRAGRRGTDSLAI
                     MVATSSPLDQYIATNPHFVFENNPEMANINPDNLLILMDHIKCAAFEIPFGIDELFTP
                     HHSTTREMLEYLAQKNILKESAGKFHWMSDIYPANDISLRSASHDNVVIVHKDNHAQV
                     IGEIDLPSAPTMIHDDAIYLHQGQQYYIDKLDWERRIAYCHEVESDYYTDAEEKTDIH
                     VLEEFESKPLSIATLSLGEVNVRTKAIMFKKIKFGTHENVGWGKINLPELEMHSTAMW
                     CSFSDEIINTMEREEAGEVFLGLSYILRNIAPLYTLSDYADIRVVFENRSHFTNQPTI
                     FVYDAIPGGVGISERVYHIFGLIAQASQEAIAACSCTHGCPACIGPMITREGIKEKVI
                     ALLKNLHHESEGQTQHILH"
     gene            complement(3312..4166)
                     /locus_tag="PK348_01830"
     CDS             complement(3312..4166)
                     /locus_tag="PK348_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013007532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2156 domain-containing protein"
                     /protein_id="HXK64982.1"
                     /translation="MAINEQSLELSHKLLLQPLLQKVTIPISEYSFANLYLFRSVHQY
                     ILITGDITAIKGVTYDGIHYIMPVMPLDEEYAEKLVQLLQPNMCLFPIHHDWLHFFTE
                     KGFTYHYNDGDSDYVYTKEKLATYSGKKLHGKRNLLYQFTAKYSVEVLPLTSQRVNDA
                     LYILDIWKKESGNDADIAACIEACMLLDELNLCGSMYYVEGKPAAFIIGEAITDDMFA
                     IHFAKAITSYKGIYQYMYNHCAKALPANYKYFNFEQDLGLEPLRFAKRSYQPDMIVTK
                     YRVSALIQ"
     regulatory      4545..4685
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            4747..6102
                     /locus_tag="PK348_01835"
     CDS             4747..6102
                     /locus_tag="PK348_01835"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HXK64983.1"
                     /translation="MGIPKSFWKSISPFQLLVSGYLLITLGGAFLLSIPIATKSGRFE
                     NYFNALFMASSGISTTGLCVVDPGSFYTFFGQVVLMVIFQIGGIGYMTLLMFILYLFD
                     IKTSLTVQLTARESLAGASLRVLGKFFIATVIFTLIFEMGGALLLALYWLKYYNLVHA
                     VWYGIFHSISAFCTAGFSLFPDNLIKYRDSIVVNAVIDIVSIAGGLGFIVLYEAVIFI
                     VNKYHNIKKLRLSIHTKLVFITTLILLMGGTFLVLITEKWDGSIDYIHRFMYSSFQVI
                     SAHTTDGFNTIDIGRMSSASLTVIMMLMFIGASPGSTGGGIKTTTFAITAKYIISLLK
                     GKSSGVVNIYEREIPQESVNKALGIFILFIVIIAIDVTIMSMTENQDFRNILFEIISA
                     LGNTGLSCGITQQLTLIGKLLLATTMFIGRVGPVTFGYFVFGSVKNTGYHYPQEDVFV
                     G"
BASE COUNT         1821 a         1078 c         1287 g         2028 t
ORIGIN      
        1 ctatgagctg tcgcccaagt gcagtaactc agtgctttat gataatcttt aacacgatgt
       61 tcatagtgtt ttgctaactc ctcaagtgaa aacagattta tatgcttttc ccagaatgta
      121 agggcctgct ccagaaggcc agagcgcttt aatgcagttg aaaacttttt aaaaagggaa
      181 tggtcgttgt cgagcaatgc atagccagca tactcaatga gttcaatgaa cagttgaata
      241 tttcgggaat ataggtgctt tgcaatattt ttatacaggt gtgtgggtac ctgatcaaac
      301 tgctttgtgt gtataagcgt gtagagggaa ttgaaaaaaa caagtagcag caccatggat
      361 gtaatatcaa tacgattgtg ttcaataacg gcagtaaggc gcgggtcata acctgtttgt
      421 tgataggtaa agtacacatc aggaatctgg tagcctggta tatcgtcggt cctggtaatc
      481 ccaagtagaa gctcttcaag tgattttaat gaacagcttg catagattga tttaaaaatt
      541 gagcgtgcgg gtttaagtaa atcaatgacg ggcatagaaa cagggaagcc ctgaatgcgg
      601 ttaatcctgt agcgcgtttt tataatgggg atgtcaaagg ttttgccatt gaaggttaca
      661 attgttttaa atttttgtaa ccgttcagcg cagtggaaaa gtactgctgg ctcttcacca
      721 tactgacgca tgaaaacctg ctcaacggtg aatttgtttt cactaaaata tcctaagcca
      781 attaaaaaag cataattgcc tgttccgctg gacagtgatg ttgtttctat atcaaaaaaa
      841 acacactggt caagtgtgat aggttccata cgatgcattg cacagtaggc tgcaatgtca
      901 gtgccagtat catcgatatg taatgatgca actggatatt gctgcacaaa cgtaatggtt
      961 tggccaaacg gtgtggtaat aatttcccca cctgcgtcct gtatccatga actatcggtt
     1021 acaggcttgc ctgagtcagt gtagtatatg ttgagtttgt ccttcagatt catggtgtaa
     1081 gtttttaagt aatgctatta ctttttcttt aatgccttct ctggtgatca tggggccaat
     1141 acaggcaggg cagccatgag tacagctgca agcggctatt gcctcctgtg atgcctgtgc
     1201 tatcagtcca aatatgtgat atactctttc ggatattcct acaccgccag ggatggcatc
     1261 atatacaaag attgttggct gattggtgaa gtgtgaacgg ttttcaaata caacgcgtat
     1321 atcggcataa tcagatagtg tatacagtgg cgcgatgttg cgtaaaatat atgaaagacc
     1381 taagaatacc tcaccagctt cttcgcgttc catagtatta ataatttcat cgctgaagct
     1441 acaccacatt gctgttgaat gcatttccag ttcaggaagg ttaatctttc cccagccaac
     1501 attttcgtgg gttccaaatt ttatcttttt aaacatgata gctttagtgc gtacgttaac
     1561 ctcgcccagg cttaacgttg caattgacag cggttttgat tcaaattctt caagcacatg
     1621 gatatcggtt ttttcttcgg catcggtgta gtaatcggat tctacttcat ggcaataggc
     1681 aatcctgcgt tcccagtcta atttgtcaat atagtactgc tgaccctgat gaagatagat
     1741 agcatcatcg tgtatcatgg ttggcgctga aggaaggtct atttcgccaa taacctgtgc
     1801 atgattgtct ttatgaacta tcaccacatt atcatgtgaa gcgctgcgca atgatatatc
     1861 atttgcaggg tagatatcac tcatccagtg aaacttgcca gctgattctt ttaagatatt
     1921 cttttgcgcc aggtattcaa gcatctcacg ggtggtgcta tggtgtggtg taaacagttc
     1981 atcaatacca aagggaattt caaatgctgc acatttaatg tggtccataa gaatgagcag
     2041 gttatcagga ttaatgtttg ccatctcagg attgttttca aacacaaagt gtggattggt
     2101 tgcgatatac tgatcaagcg gtgagcttgt ggcaaccata attgcaaggg aatcggttcc
     2161 ccgcctgcca gcacgacctg actgctgctg cagtgaagct attgagccag gataaccaac
     2221 agagatggca caatccagca taccaatgtc aatacctaac tcaagtgcgt tggttgagac
     2281 aaccgcgttt atcttttttt cacgtaaacc cttttcaata gatctacgtt cattgggtaa
     2341 atatccgcca cgatatgcgg caactgaaaa ccctttacac tgttctttta aatatgttgc
     2401 gatgatctca acgcgcaatc tgcttcgaca aaaaactatt gttgaaatgt tattcttcac
     2461 gcacagtgtg ccaacctttg cagcttcctt cacggctgaa gcccgtatac ccaaagcctt
     2521 gtcaaccaca ggtggattat atattacaaa atgcttttta ccgcagggtg caccgttatt
     2581 gtcgattaca gtaaatggca tttcaaaaag cttttcagca agttcttttg gattggcgat
     2641 agttgctgag cagcagataa attgcgggtg gctgccataa aatgagcata tacgtttaag
     2701 tcttcgcatg agattggcaa aatggctgcc aaagactcct ttataggtat gaatttcatc
     2761 aacaacgacg tactgcaaat tttcaaaaag cttaacccat atagtatgat gtggcagaat
     2821 acctgaatgc agcatatcgg gattggtgat gacaatatta cctgctgtgc gaatagcttt
     2881 acgagctgac tgtggtgtat caccgtcaaa ggtgtatgtt ttaatggata aaccggtgat
     2941 cttcataatc tcttgaagtt cagaaagctg atcctgcgat agggcttttg tggggaatag
     3001 gtacagcgcc cgtgcatcgg gatgtttggc cataaaatta agcacgggaa gattgtagca
     3061 tagcgttttg cccgaagctg ttggcgttac taccacaata ttttcttttt tttgaactgc
     3121 atcatatgct gagcgctgat ggctgtaaag attttctatg ccacgctgca tgtatacatt
     3181 gcggattcgt tcgtctatgt catccggata tggtgcatag acagcttgcc tggcaggtac
     3241 cgtataccat gctgcaacct gtttcataaa cggttcatag tttttaagat attcaataag
     3301 ctgttcaata gtcattggat taaagcactc acacgatatt ttgtcactat catatcgggc
     3361 tgatatgaac gctttgcaaa gcgcagcggt tctaatccta aatcctgttc aaagttaaaa
     3421 tatttataat ttgcgggcaa tgcttttgca cagtgattat acatgtattg ataaattcct
     3481 ttgtaacttg ttatagcttt tgcaaaatga attgcaaaca tatcatcagt tattgcttct
     3541 ccaatgatga atgcagcagg ttttccttca acgtagtaca tgctgccgca cagatttaat
     3601 tcgtcaagca acatacacgc ttcaatgcac gcagcaatat ccgcatcatt gccggattcc
     3661 ttcttccata tgtcaagtat atataatgca tcattaactc tctggctggt taatggaagc
     3721 acttctacag aatattttgc cgtaaactga tacagaagat ttctttttcc atgaagtttt
     3781 ttcccggaat aggtggctaa cttttctttg gtatagacat aatcggagtc gccatcattg
     3841 taatgatagg taaagccttt ttcagtaaaa aaatgcaacc agtcatggtg aatgggaaat
     3901 agacacatat tgggttgcag gagttgtacc agtttttcag catattcttc atccagcggc
     3961 ataacaggca taatatagtg tataccgtca tatgttacac ctttaattgc tgtgatatcc
     4021 cctgttataa ggatgtactg gtgaactgaa cgaaaaagat acaggtttgc aaatgaatac
     4081 tcagatatag gtatggttac cttttgcaaa agaggctgaa gtaataattt gtgcgatagt
     4141 tctaatgatt gttcattgat agccatagta gccatttttt atatagatat atatttacca
     4201 acaatgtatt gtgtatatat actttttcaa tctctttttt tcaaatttac aagaacaatc
     4261 cacaggaggt taaatattaa aagctaccgg tgagatgtgg agttttgtga cacgataatt
     4321 tctcttagct tgcaaaagat gtaatgaaat cattgttgct ttttcataaa ttatgatgct
     4381 gagaataaaa aagcatggtt acgtataacg cattataaca aaatgtgtat agctacatca
     4441 ctttaataat tgaattaatt tcttttaaga ataaatataa aaaataatat ttattattga
     4501 cgagataaag aatttaaagt atattaatag ccaattttac atgctgcata aaaaagccta
     4561 aatccattac agcagcaatg gatgcggggg acccaatttt tggggcgtat tccttttaca
     4621 taaggatagg gtacttccaa acccgagccc gtcagctaac ctcgtaggct tttggaaggg
     4681 caagagcact ataaaactgc tttttgcggt tttttttatg cctttgcccc gcataggagg
     4741 ggtaatatgg gcattccaaa aagcttctgg aagtctattt caccattcca gttattagtt
     4801 tcaggatatc tattgattac actgggtgga gcattcctgt tatcaattcc aatagcaact
     4861 aaaagtggtc gctttgaaaa ttattttaat gccttattta tggcatcctc aggtataagt
     4921 acaacaggtt tgtgtgtggt tgatcccggg agtttttata ctttttttgg gcaggttgta
     4981 ttaatggtta tattccagat tggcggaata gggtatatga cattgcttat gtttatattg
     5041 tatctgtttg acataaaaac atcattaaca gtacaattga ctgccagaga gtcgttagca
     5101 ggtgcatcac tgagagtgtt gggtaagttt tttattgcaa cggtcatttt tacgctaatc
     5161 tttgagatgg gaggtgcatt attactggca ttatactggt taaagtatta taatttagtt
     5221 catgctgtat ggtatggtat atttcattca atttcagctt tttgtacagc aggatttagt
     5281 ttattcccgg ataatttaat aaaatataga gatagtattg tagtaaatgc tgtgattgat
     5341 attgtatcaa ttgctggtgg acttggtttt attgtccttt atgaagcagt aatatttatt
     5401 gtcaataaat atcataacat taaaaaactt cgtttatcga ttcatacaaa gctggtattc
     5461 attactacct tgatactctt aatggggggt acattcttgg tattaataac tgaaaaatgg
     5521 gatgggtcta tagattatat tcatagattt atgtattcgt catttcaggt aatatctgca
     5581 catacaactg atggatttaa tactattgat attggtagga tgagttctgc cagccttaca
     5641 gtgataatga tgttaatgtt tattggtgct tcgccaggta gtacaggtgg tggaattaaa
     5701 acaacaactt ttgctattac agctaagtat attatttcac tgttaaaagg gaaaagcagt
     5761 ggtgtggtta atatttatga aagagagatt cctcaggaat cagtgaataa agcactgggt
     5821 atttttattt tatttatagt aataattgca attgatgtaa caataatgag tatgacggaa
     5881 aaccaggatt tcagaaatat attgtttgaa ataatctctg cattaggcaa tacagggctt
     5941 tcttgtggta ttacacaaca attgacatta attggcaaac tattgttggc cactacgatg
     6001 tttattggaa gagttggacc tgttacattt ggttactttg tatttggtag tgttaaaaat
     6061 acagggtatc actatccaca agaagatgta ttcgttggat aaactggtgg tacaaaagta
     6121 ttattctatg aaggaatgat aatacaatat tccatgtgtt ttggtttgac atcatcaatt
     6181 atttttagaa aacattttta cataatgata gagt
//
LOCUS       DAOSZP010000048         6161 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_10755_length_6161_cov_2.869636, whole genome shotgun sequence.
ACCESSION   DAOSZP010000048 DAOSZP010000000
VERSION     DAOSZP010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6161
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10755_length_6161_cov_2.869636"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..482)
                     /gene="ispF"
                     /locus_tag="PK348_01840"
     CDS             complement(<1..482)
                     /gene="ispF"
                     /locus_tag="PK348_01840"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HXK64984.1"
                     /translation="MHNLTAYRTGIGFDAHRLVQGRKLIIGGVHIPYEYGLLGHSDAD
                     VLTHAIIDALLGASNLGDIGTLYPDTSSQFKDKYSIDMLKDTYQLLTKHNIEIINIDA
                     VILCQKPRIAPYSEQMKEILSAACGNLPVSSISIKGKTTEGMGFTGTGEGIAALATCL
                     V"
     gene            complement(515..814)
                     /locus_tag="PK348_01845"
     CDS             complement(515..814)
                     /locus_tag="PK348_01845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HXK64985.1"
                     /translation="MEINLNNKGNLVEVKVKGDIEMMSIKEFKEKLLAVGEDNDVDIA
                     LDLSEVDYIDSSGVGVLISLNKIQKKKGKKLELINVSPKVLNVLKLSSLAEVFNL"
     gene            1061..2938
                     /locus_tag="PK348_01850"
     CDS             1061..2938
                     /locus_tag="PK348_01850"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXK64986.1"
                     /translation="MQLKKTYTDVYESKKIDFQKLCEIIKPGSRVFLSSGPAMPVYTV
                     EQMMTSDAPTLQDLEIIHLITLGNYLKTDSNLYKFRLKTFNTGESISKDIEQGKADFV
                     PAHLVEIPFIFSSGAIGVDVAIIQVSPPDHRGFMSLGIAIDVANIVIENAKTVVAEVN
                     PYVPVTMGDTAVHIDAIDYIIESKVPLIEREYKRYDEIMDKIGWHVANLIDDGSVIAL
                     HVGRQFDAIAKHLHNKRELKVYSHVVSDWIIDLIESGAVKIDRGIRNLAPVTLSYCYG
                     TRMLYDYVDRNPMFEFLPLMRLLYPTVVQRIPKLVSVMNVKKIDLSGESVVFYSGDNL
                     LSGYESKLNFAVGAAFSRGGKAIVALRSIDQHGDSNIVIRHTETEQVRSTLGVTRYVV
                     TEYGVANLFGKSIRERALALIDIAHPNHRLSLLNQAKEAGLVYPDQIYVIENIINYPV
                     TLETMKTFKDGLLVKFRPIKPSDEDMMRRLFYQFSDESKYLRYFARIRTMPHKQMQSY
                     VNIDYDRILSIVGIIQHAGTERIIAEARYGYDDNEKTYEMAFIVDEEFQGKGIATFMA
                     RYLMKIAKERNIEKMSANVLPQNDKMMKVFEKCGVPFTQRFEDGVMHFDFHLQKADIV
                     I"
     gene            complement(2931..3572)
                     /gene="thyX"
                     /locus_tag="PK348_01855"
     CDS             complement(2931..3572)
                     /gene="thyX"
                     /locus_tag="PK348_01855"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012882510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HXK64987.1"
                     /translation="MEIYSPKVTLLAITPNAEKLIEEAGRTCYLSLDRITEGSETNFI
                     RRCIMNGHHSILEHASASFRVLGASRAFTHQMVRHRLASFSQQSQRYVNEEEFNFIIP
                     PSIAANNEAKTIFTDFMEQARIIYKKLRELSIKKEDARFVLPNALESQIVISANFREW
                     RHIFSLRLEKAAQWEIRSVCMQMLKILQDAAPSVFGDFIIDEATCTAKSNLIK"
     gene            complement(3587..4189)
                     /gene="rsxA"
                     /locus_tag="PK348_01860"
     CDS             complement(3587..4189)
                     /gene="rsxA"
                     /locus_tag="PK348_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009424153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HXK64988.1"
                     /translation="MEIKILFTILISTIFINNYVLSRILGLCPYLGVSKKLDSAIGMG
                     LAVIFVMTMASFFTFIIYKFVLIPFHIEYLRTIAFIIVIASLVQLVEMVIEKISPALY
                     QALGIFLPLITTNCAVLGVAVLNIESGFITQEFGLLNSVVQGFGAGVGFTLALLLMAG
                     IRERLEIAMVPENLKGLPITFITAGLMAMAFLGFSGMKIF"
     gene            complement(4214..4822)
                     /locus_tag="PK348_01865"
     CDS             complement(4214..4822)
                     /locus_tag="PK348_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HXK64989.1"
                     /translation="MDYFKEFFKGVWQKNPILVLGIGMCPTLAVTTSASNAIWMTIAT
                     TFVLLGSNILVSAIRTIVPSHVRIPIFITIIASFVIIVELTLKAFQPDVYKALGIFIP
                     LIVVNCIILGRAEEFASKNSVLSSILDALGMGLGFGLTLFILATIREVLGANKLFGYV
                     LIPGMKPAILMILAPGAFFTLGLLLWAMNVIKSRKALFKRTE"
     gene            complement(4822..5499)
                     /locus_tag="PK348_01870"
     CDS             complement(4822..5499)
                     /locus_tag="PK348_01870"
                     /inference="COORDINATES: protein motif:HMM:NF016121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HXK64990.1"
                     /translation="MKPADIVKATIIISVIGFISAFSLSFMKKITQEPIAKQEQEKKL
                     AALALVLPGYTVQQEKTVTIDDSPFTYWIATKEENRIVKKAWAFLAEKKGYGGPVQSI
                     VGVDESNTILGFTILSQNETPGLGARCVEVASNETFVDHFITKNLTSKEEVSWFQEQF
                     IGLNASKPIAIQKKGDWHKEMKDELIQHNAVTAITGATITTKAVTQSIEEGIQKLHKA
                     LTLQEVQ"
     gene            complement(5838..>6161)
                     /locus_tag="PK348_01875"
     CDS             complement(5838..>6161)
                     /locus_tag="PK348_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HXK64991.1"
                     /translation="VSYIATVALIMGIYYYFQGIPNLQYAILFHVLSGGLILGAFFMA
                     TDMVTSPVTKTGMIIFGIGCGILASVIRLWGGYPEGVSYSILLMNAVTPLIDRLTRPR
                     VFGTR"
BASE COUNT         1899 a         1172 c         1215 g         1875 t
ORIGIN      
        1 accaggcatg tagccagtgc agctatgcct tcacctgttc cggtaaaacc catcccttca
       61 gttgttttac ctttaattga gatagatgat actggcaaat taccgcaggc agctgaaagt
      121 atttctttca tctgttctga atagggtgca atcctgggtt tctggcatag tatgactgcg
      181 tcaatattta taatttctat attatgtttg gttaaaagct gatatgtatc tttaagcata
      241 tctatgctgt atttgtcttt aaactggctg ctggtgtcgg gatatagcgt gccaatatcg
      301 cctaagttgc ttgcgccaag caatgcgtca ataattgcat gtgttaaaac atcagcatct
      361 gagtgcccca gcaggccata ttcatacggt atatgcacac caccaataat aagctttctg
      421 ccctggacaa gtcggtgagc atcaaagcca atgcccgttc tataagctgt gagattatgc
      481 atataaaggt tctggtataa ggaactatcg ccaattataa attaaaaact tcagctagcg
      541 agcttaattt caatacatta agcactttgg ggctgacatt gatgagttcc agttttttgc
      601 cttttttctt ctgtatttta ttcaggctga taagaacgcc aacacctgaa gagtcaatat
      661 aatcaacctc agaaagatca agtgctatat caacatcatt gtcttcacca actgcgagga
      721 gtttttcttt gaactctttg attgacatca tctcaatatc gcctttaact ttaacctcaa
      781 ccagatttcc cttattgtta aggttgattt ccatgtggtt acccctgtgt atattcaagt
      841 cttaccagac taatataaac gcatatgtgc tattgtgtca ataattaaat ctttgttaca
      901 gtaaaaatat atttgaatga catgtgatta agaaataata atcattaaaa accgtgttta
      961 ctgcctaaat aattgacaaa aataaaaaat aatgtagaat aataaacagt agtggtggct
     1021 ataggacctg ttaatacaat actacactaa ggaatataca atgcaattaa aaaaaacata
     1081 taccgatgtt tatgaatcaa aaaaaattga tttccagaaa ctatgcgaaa ttataaaacc
     1141 tggttcccgt gtttttttga gcagtggccc ggcaatgcct gtgtatactg ttgaacagat
     1201 gatgacatca gatgccccca cactgcagga cttggagata atacatctta ttacactggg
     1261 caattacctt aaaaccgaca gcaatcttta taaatttcgc ctgaagacct tcaataccgg
     1321 tgaaagtatc agcaaagata ttgagcaggg caaggcagat tttgtgcctg cgcatttagt
     1381 ggaaattcca tttatttttt ccagtggtgc tattggtgtg gatgttgcta ttatacaggt
     1441 ttcgccacct gaccacaggg ggtttatgag tttgggcatt gcgatagatg ttgccaatat
     1501 agttattgaa aatgcaaaaa ctgttgtagc agaagtcaac ccgtatgttc ctgttacgat
     1561 gggtgacacg gctgtgcata ttgacgctat agattatatt attgaaagca aagtgcccct
     1621 tattgaaagg gaatataagc gatacgatga aataatggat aagattggat ggcatgttgc
     1681 caatcttatt gatgatggtt cggtgattgc attacatgtt ggaagacaat ttgatgcgat
     1741 agcaaagcat ctgcacaaca aaagggaatt aaaggtttac agccatgttg tctctgactg
     1801 gattattgac cttattgaat caggagctgt gaagatagac cgaggaatac gcaatttagc
     1861 acccgtgacg ttgagctatt gttatggcac ccggatgctg tatgactacg ttgatcgtaa
     1921 tcccatgttt gagtttttac cactcatgcg ccttctatat cctaccgttg tgcagcgcat
     1981 acctaagctg gtgagtgtca tgaatgtcaa gaagattgat ctttctggtg agtcagtagt
     2041 attttactca ggtgataatt tactgtccgg ttatgaaagc aagcttaatt ttgccgttgg
     2101 tgcagctttt tcccgtgggg gcaaagctat agtggcatta cgttctattg atcagcatgg
     2161 cgatagtaac attgtaatac ggcacacaga aaccgaacag gtacgatcaa ccttaggtgt
     2221 tacccgatat gtagtaaccg aatatggtgt ggcaaacctt tttggcaaat ccattcgtga
     2281 gcgggcactg gcgctgattg atattgctca tcccaatcat aggctttcat tattgaatca
     2341 ggctaaggaa gcggggctgg tgtatcctga tcagatatac gtaattgaaa atattataaa
     2401 ctatcctgta acccttgaaa caatgaaaac attcaaggat ggtctgctgg taaagttcag
     2461 gcctatcaag ccatctgatg aagatatgat gcgaaggctt ttttatcagt tctcagatga
     2521 atcaaaatat ctgcggtatt ttgcgcgtat acggaccatg ccgcataagc aaatgcagtc
     2581 ttacgtaaat attgactatg acagaatatt gtcgatagtt ggcataattc agcatgctgg
     2641 cactgaacgg attattgcag aagcgcgcta tggatatgat gacaatgaaa aaacgtatga
     2701 gatggctttt attgttgatg aggaattcca gggtaaaggt atagccacat ttatggcacg
     2761 atatttaatg aagattgcaa aggaacgaaa tattgaaaag atgtcagcca atgtgttgcc
     2821 gcaaaatgat aagatgatga aagtttttga aaagtgtggt gtgccgttta cccagcgttt
     2881 tgaagatggc gtaatgcact ttgattttca tttgcagaaa gctgatattg ttatttaatg
     2941 agattggatt tagctgtaca ggttgcttca tcaatgatga aatcaccaaa aactgatggt
     3001 gctgcatctt gcaaaatttt aagcatctgc atgcacacgc ttcgtatttc ccactgtgct
     3061 gccttttcaa ggcgcaaaga aaaaatatgt cgccactccc tgaaattagc tgaaatgact
     3121 atctggcttt ccagtgcgtt gggaagcaca aaacgcgcat cttctttttt tatgctcagt
     3181 tcccgcaact ttttatagat aatgcgcgcc tgttccataa aatcagtaaa gatggttttt
     3241 gcttcattat ttgctgcaat tgagggtgga atgataaaat taaattcttc ttcgttaacg
     3301 tagcgctgtg attgctgcga aaagcttgca agcctgtgtc gcaccatctg atgggtgaat
     3361 gcgcgggaag cccccagtac cctgaatgat gctgatgcat gttcaagtat tgagtggtga
     3421 ccgttcatga tgcaacggcg aatgaaattt gtttcacttc cttcggttat tctatccaga
     3481 gaaaggtaac aggtacggcc tgcttcctca ataagctttt cagcattggg tgttatagct
     3541 agtaaggtta cttttgggga ataaatttcc atacactatc gcttacttaa aaaattttca
     3601 tgcccgaaaa gcctaaaaat gccattgcca tgagaccagc ggtaataaac gtgatgggca
     3661 gacctttaag gttttccggg accatggcaa tttcaaggcg ttcacgtatg ccagccataa
     3721 gaagcagagc gagtgtgaaa ccaacgccag caccaaaacc ctgcaccaca gaattaagca
     3781 ggccaaactc ctgtgtaata aagcctgatt ctatattaag aacagctact cccaacacag
     3841 cacaattggt tgtaataagt ggtaaaaata ttccaagcgc ctgatacagg gcaggggata
     3901 ttttctcaat aaccatttca acaagctgta caagagaagc tataacaata ataaacgcaa
     3961 tggttcgcaa atattcaata tgaaaaggta taagcacaaa tttgtaaata ataaaggtaa
     4021 agaaagaagc cattgtcata acaaagataa cagccagccc catgccaatg gccgaatcca
     4081 attttttaga aacacctaaa tagggacaaa ggcccagtat tcttgaaaga acataattat
     4141 ttataaagat agtacttatt agtattgtaa aaagtatctt aatttccata acgatagtac
     4201 ctcacctgta cttttattca gtacgtttaa aaagagcttt ccgtgatttt ataacattca
     4261 tagcccacag gagcagtcct aaggtaaaaa atgcacctgg tgcaagtatc attagaattg
     4321 ctggcttcat gccaggaatt aaaacatatc caaaaagctt atttgcacct aacacctcgc
     4381 gaattgttgc aaggataaag agggttaaac caaaccccaa ccccatacct aatgcatcaa
     4441 gaattgatga tagaactgaa ttctttgaag caaactcttc ggctcttccc aatatgatgc
     4501 agtttactac aatgagtgga ataaatatac caagtgcttt gtatacatca ggctgaaatg
     4561 ccttaagtgt taattcaaca atgataacaa agctggctat tatggtgata aaaattggta
     4621 ttcgaacatg tgaagggaca atggtacgta tggccgatac aaggatattg gaccccagta
     4681 atacaaacgt tgttgctatt gtcatccaga tagcattgga tgctgaagta gtaactgcaa
     4741 gtgttggaca catccctatc cctaatacca ggattggatt tttttgccat actcctttaa
     4801 aaaattcctt aaagtaatcc attattgtac ctcttgcagt gttaaagctt tatgtaattt
     4861 ttgtatgcct tcttcaatac tctgtgtaac tgctttagtg gtgatagtgg cccctgttat
     4921 tgcagtaacg gcattatgct gaattaactc atctttcatt tctttgtgcc agtcgccttt
     4981 tttttgaata gcaatgggtt tgctggcatt caagccaata aattgttcct gaaaccagga
     5041 cacctcttct ttactggtaa gattttttgt tataaaatgg tcaacaaatg tttcattgga
     5101 tgcaacctca acacagcgtg caccaagtcc cggagtttca ttctgtgata gtatggtaaa
     5161 acctaaaatt gtatttgatt catctacccc aacaattgat tgaactggtc cgccgtatcc
     5221 ttttttttca gctaaaaaag cccatgcttt ttttactatc ctgttttcct cttttgttgc
     5281 tatccagtaa gtaaatggag aatcatcaat ggtaactgtt ttttcctgct gtactgtata
     5341 accgggtaat acaagagcaa gagccgccag cttcttttcc tgttcctgtt ttgctattgg
     5401 ttcctgagta atctttttca taaatgataa tgaaaaagca gatataaatc caataacact
     5461 tataataatc gtagccttta cgatatcagc aggtttcatg taataattgt tcctctcatc
     5521 ttaaaaatat gatgtatagc aattatcatt ttaaatcccg atttcatcgg ggtgagaaat
     5581 ctttttaaca gcgaaggtca tcctgaggaa tcccaacttt atcgggacga cgaaggacct
     5641 tgtccttact actcagagat tcttcgctgc gctcagaatg acaaagaaat taaatacgat
     5701 ttctcctcac ttcgttcgtc gaaatgacag tatgaaaaat tacttcgttc gtcttagtaa
     5761 cagcgtaaaa ataatcatag tgacagagca aaaatgttcg tcgaaataag taattccaaa
     5821 aatattataa tcagttacta tcgggtacca aatactcttg gtcgtgttaa cctgtcaatt
     5881 aacggtgtta cagcattcat caatagaatt gaatatgata caccttcagg gtatccgccc
     5941 cataagcgta tgaccgatgc cagtatacca catcctatgc caaatataat catccctgtt
     6001 tttgtaacag gtgaggttac catatcggtt gccataaaaa aggcacccaa aattagtccg
     6061 cccgacagta catggaaaag tattgcatac tgaagattgg ggatgccctg aaaataatag
     6121 tatattccca taattagtgc aaccgttgct atataggata c
//
LOCUS       DAOSZP010000049         6090 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_10926_length_6090_cov_3.593041, whole genome shotgun sequence.
ACCESSION   DAOSZP010000049 DAOSZP010000000
VERSION     DAOSZP010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6090
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10926_length_6090_cov_3.593041"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..984
                     /locus_tag="PK348_01880"
     CDS             <1..984
                     /locus_tag="PK348_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018975661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HXK64992.1"
                     /translation="PEFKYAAAKYLKEVFGVEGIDPETEVNHSIGSKPALAMLPSAFI
                     NPGDVCLMTVPGYPVFGTHTEWYGGQVYNIPLLKNNNFLPDLDSIPADIVKKAKVLVI
                     NYPNNPTGAVATEDFYKKVIEFALKNKIMVVQDAAYAALTYNGKPLSFLSIPGAKEVG
                     VEIHSLSKAFNMTGWRMAFVAGNPLVVKAFANVKDNYDSGQFKAIQKAAITALEHPEL
                     TEKIKEKYKRRLSSLVTTLRRLGFDAHMPGGTFYLYVQAPKKTKSGIVFDNAESFSQY
                     LIKECLISSVPWDDVGNFVRFSATFEAKDQNDEQRILKEVETRLGGLAFEF"
     gene            1000..3261
                     /locus_tag="PK348_01885"
     CDS             1000..3261
                     /locus_tag="PK348_01885"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HXK64993.1"
                     /translation="MTRKTLLLITGGAVAIILLAIGIYRFYFLPVSYQDTITEGVAFP
                     VAIYRLDKGYPLIVAENRDDAIFALGIVHAQDRLLQMEFLRSLATATGVQDDLSQYES
                     IDRLVKAVGLPLKAKTLLSELSPQYLQTLQKYVDGINTYTFKMGHASKNKTHWSVEDI
                     VCITLFLEFCNAFVTNSELFFPVEDKDFPLLYRELANKKIVHSYRKQDFTIIKEIQKL
                     QEVVASIFGLYGSYTRGFAVAIPSRLMYTDSATLCSSYEHSVSLYPMIYPVNFQVAGK
                     VIEGYTCAGLPYVVAGKTNTLRYASFSLFADSQVFCKYQTQTRNNVLYYNSAAGYKEL
                     QCQPDASQCVSRFTDSGPVISDAFEYNLKDAIVAISYVSPAKGYIESMIDVAFANSIQ
                     DVIQIVQKYKGQPRVFIVQDDTRSYQIIAGSAMLDNESVLLSPEKALLLKNAPALFEI
                     KQADMCMAGSEFVENPSPAIQRVMVFNNTGRLSSLSHELSHYNEMQYKDIEKILYSVT
                     YPDAKEYLVIINNLLDKMPITSAKLAKVYFRDWDLTMGSSEVAPTLFNAIIAAMIRET
                     FADELPNAVKNMLTHYSIFEPEFKRIFKEDRSVYFDDITTLEKIESRDMIFDRAFLHS
                     LKPFNYTIGPYIDEWKWGKVHSATIHYLDSGSLVKNVMGKKKTIAFNGCASTVYRSLS
                     EQKNFSVDTVTVSCFYMDKMVHRASLSTSISSNPASHYYNEFVAKDSFKDINLSKRNE
                     LFLIKPVKEIQKQ"
     gene            complement(3273..3345)
                     /locus_tag="PK348_01890"
     tRNA            complement(3273..3345)
                     /locus_tag="PK348_01890"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3310..3312),aa:Ala,seq:cgc)
     gene            3453..3935
                     /locus_tag="PK348_01895"
     CDS             3453..3935
                     /locus_tag="PK348_01895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK64994.1"
                     /translation="MLDFILDFIAKLLSYSMKFINWVADILMRLIDYCIGIFRELEIQ
                     EKIIILLSIVSVIIPILPIARFYIFESWYYVNNPLAVYFIGIIIIMVISTIVQKPWIL
                     ITRLIIIVYYLAWMIYLPAGNLITRAKPYELAYGYYINIAISIVYIGLCVFSLLSQRR
                     "
     gene            complement(3940..4347)
                     /locus_tag="PK348_01900"
     CDS             complement(3940..4347)
                     /locus_tag="PK348_01900"
                     /inference="COORDINATES: protein motif:HMM:NF018391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1178 family protein"
                     /protein_id="HXK64995.1"
                     /translation="MISFDLECNNGHKFEGFFKDYNAFDDQMKAGLVECPVCNSREIK
                     RLYTGCSIQAKSSLLEKKQPTFFEMVRMVESYIKEHFENVGDRFPEVARAIYYGIEEE
                     RNIYGNASKEEMKELIDEGIPVLPLPKIDDIEN"
     gene            4453..5676
                     /locus_tag="PK348_01905"
     CDS             4453..5676
                     /locus_tag="PK348_01905"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HXK64996.1"
                     /translation="MTQWWEKSIIYNLDYLANNGILFKNAFVTTSLCSPSRASFLTGK
                     YASRHGVINNFSPWKAENVTFMELLKQGGYDTAFIGKWHMPGKGLPHLRGVDLFISFT
                     RKDGQGDYYNCPLFVNHKPVSNNKEYITEELTDYALEFISQPRKNPFCLYLSHKAVHH
                     DWKPPRHLKGLYKNEKIDFLAPESDKWNTWSGNNWLEGSMGSMHDIYKRYCECLVSVD
                     EQIGRIIQLLNKMGILENTVIVYAGDNGYMWGEHRLYAKHWPYEESIRIPFIFFYPGI
                     VKKPGKREQMILNIDLAPSLLQLAGIRIPDWMQGESFVPIVKNPLSPGRKSFMYELFK
                     DFPFGGRVPPNKTLRTERYKYIEWQACRGPELYDLLEDPKEMNNMIGTQKAKKIVPML
                     KKELEEIKIKYGAID"
BASE COUNT         1959 a         1001 c         1194 g         1936 t
ORIGIN      
        1 cctgagttta aatatgcagc agctaaatat ttaaaagaag tatttggagt tgagggaatt
       61 gatcctgaaa cagaagtaaa ccattcaatt ggttcaaagc cggcacttgc catgttgcct
      121 tcagcattta ttaatccggg tgatgtatgt ttaatgacag ttcccgggta tccagttttt
      181 ggtactcata ccgagtggta tggtgggcag gtgtataata tcccattatt aaaaaataat
      241 aattttttgc ctgatttgga ttcaatccct gcagatattg taaaaaaggc aaaagttctt
      301 gttataaatt accccaataa tcctacagga gcagtggcta ctgaggattt ttataaaaag
      361 gtcattgagt ttgcacttaa aaataagatt atggtggtac aggatgccgc ctatgcggcc
      421 ttgacctata atgggaagcc gctatcattt ttaagtatcc ccggtgcaaa agaagttggg
      481 gttgaaatac attcgctatc aaaagctttt aatatgacag gctggcgaat ggcttttgtt
      541 gcaggcaacc ctcttgtagt aaaagcattt gcaaatgtaa aagataatta tgattcaggg
      601 caattcaagg ccattcagaa ggctgcaata actgccctgg aacatcctga gctaactgaa
      661 aaaataaaag aaaagtataa aagaagacta tcttcacttg ttacaacctt acgacgtttg
      721 ggctttgatg cacacatgcc tggtggaaca ttctatctgt atgtgcaggc accaaaaaaa
      781 acaaaaagtg gaattgtttt tgacaatgca gaaagttttt cgcaatattt aataaaagag
      841 tgcctgatat cctctgtgcc atgggatgat gtgggtaatt ttgtccgctt ttcggctacg
      901 tttgaagcaa aggaccagaa tgatgaacag cgtattttaa aagaggtgga aaccagatta
      961 gggggactgg catttgaatt ttaatgggtg gttataccta tgaccagaaa aactctttta
     1021 ctgattacag gtggtgctgt tgcaattata ctgctggcaa ttggcattta ccggttttat
     1081 tttttacctg tttcgtatca ggataccata accgaaggtg ttgctttccc tgtagcaatt
     1141 tatcgacttg ataaaggcta tccgctgatt gtagcagaaa accgtgatga cgcaatattt
     1201 gcattaggaa ttgttcatgc acaggatagg ctcttacaaa tggagttttt gcgaagtctt
     1261 gcaactgcta caggtgtcca ggatgatttg tctcaatacg aaagcataga caggctggtc
     1321 aaagctgtag gattgccctt aaaagcaaag acgttattgt cggaactatc gccacagtac
     1381 ttacagactt tgcaaaagta tgtggatggc atcaatacct atacatttaa gatggggcat
     1441 gccagtaaaa ataaaacaca ctggagtgtt gaagatattg tttgtattac cctgttttta
     1501 gaattctgta atgcgtttgt tacaaacagt gaacttttct ttcctgttga ggataaagat
     1561 tttccactgc tttaccgtga attagctaat aaaaaaattg tccatagcta cagaaagcag
     1621 gatttcacta ttatcaaaga gatacaaaaa ctgcaagaag ttgttgcatc aatttttggg
     1681 ctatatggtt catatacaag aggatttgct gttgctatcc cctcacgttt gatgtacact
     1741 gattctgcta cactgtgttc aagttatgag cacagtgttt ctctgtaccc catgatatat
     1801 cctgtaaatt ttcaggttgc aggcaaagtt attgaagggt atacatgtgc aggattgccg
     1861 tatgttgttg cagggaaaac caatacactc cgctatgctt cattctcatt atttgctgac
     1921 agtcaggtgt tttgcaaata tcagacacaa acacgcaata atgtattgta ctataacagt
     1981 gctgcagggt ataaagaatt acaatgtcag cctgatgcat cgcaatgcgt atcacggttt
     2041 accgatagtg gcccggttat atctgatgct tttgaatata atctgaaaga tgcaattgta
     2101 gcaatatcgt atgtgtcccc tgcaaaaggg tatattgagt cgatgattga tgttgctttt
     2161 gcaaattcaa tacaagatgt aatacagatc gttcagaaat ataaaggcca gccgcgtgta
     2221 tttattgtgc aggatgatac caggtcatat cagattattg ccggaagtgc gatgctcgat
     2281 aatgaatctg tattgctaag cccggagaag gcattattgt taaaaaatgc tcctgcctta
     2341 tttgaaataa aacaggctga tatgtgtatg gctgggagtg aatttgttga aaacccatca
     2401 cccgcaatcc agcgtgtaat ggtatttaac aatacaggta ggttatcctc actatcccat
     2461 gaactgtcac attacaatga gatgcaatat aaggatattg aaaaaattct ttatagcgtt
     2521 acataccctg atgctaagga atatctggtt attatcaata atttgctgga taaaatgccc
     2581 attacgtctg ctaagttggc aaaagtctat tttagggatt gggatttgac catgggaagc
     2641 agtgaggtgg ctcccacgct cttcaatgcc attattgctg caatgattcg ggaaacattt
     2701 gcagatgagt tacccaatgc agtaaagaat atgcttacac actattcaat ttttgaaccg
     2761 gagtttaaaa ggatttttaa ggaggataga tcagtatatt ttgatgatat aacaactcta
     2821 gaaaagatag aatcgcggga tatgattttt gatagagcat ttttgcattc attaaaacct
     2881 tttaactata caataggccc gtatattgac gaatggaaat ggggcaaagt tcacagcgca
     2941 acaatacatt atcttgattc aggttcactg gttaaaaatg taatggggaa aaagaaaaca
     3001 atagccttta atgggtgtgc ttctactgta taccggtcac tctctgagca aaaaaacttc
     3061 agtgttgata ctgtaaccgt atcatgtttc tatatggata aaatggttca ccgggcttct
     3121 ctatcaacga gcatatccag caatccagct tcccattatt ataatgagtt tgtagcaaaa
     3181 gattcattta aggatattaa tttgagcaag aggaatgagc tttttttaat aaaaccagtg
     3241 aaagaaatcc agaaacagta gtgtaacaca attggagctg atcggattcg aaccgacggc
     3301 ctctagagtg cgattctagc gctctcccag ctgagctaca gccccattgt atatacgtca
     3361 acctaagggc atattggtat ttaagtcaat tgatttttat atagtcagtg attacaatct
     3421 tttaggataa tattacaaaa aggactccag gtatgcttga ttttatttta gactttattg
     3481 caaagctact atcgtatagt atgaaattta ttaattgggt agctgatatt ttgatgcggt
     3541 tgatagatta ttgtattgga atttttcggg agctagaaat acaggaaaaa ataattattt
     3601 tactttcaat agtatcagtt atcataccca ttttgcctat tgcacgattt tatatttttg
     3661 aaagctggta ttatgttaat aacccactgg ctgtttattt tatcggtatt atcataataa
     3721 tggtgatttc aaccatagta caaaaaccct ggatattgat tacaaggctt atcatcattg
     3781 tatactattt agcgtggatg atatatctgc ctgcaggcaa tcttatcacc agggcaaaac
     3841 catatgaatt agcgtatggg tattatatta atattgcgat ttcaatagta tatatagggt
     3901 tatgcgtgtt ttcgcttttg agtcagcgcc gatagcttat taattttcta tatcatcaat
     3961 tttgggcaaa ggcaataccg gaatcccctc gtcaatcaac tctttcattt cttctttgga
     4021 tgcattacca taaatattgc gctcttcttc aataccatag tatattgcac gggcaacttc
     4081 aggaaacctg tcgccaacat tttcaaaatg ttctttgata tatgattcaa ccatgcgaac
     4141 catttcaaag aaggttggtt gttttttttc aagtaatgag ctttttgcct gtattgagca
     4201 tccggtatac agtctcttta tttcgcgcga attgcacacc gggcattcaa caaggcctgc
     4261 cttcatctga tcatcaaagg cattgtaatc cttaaaaaat ccttcaaatt tatgcccatt
     4321 attacattct aagtcaaaac tgatcatggt atggactctt caccgtattg tattaaagcc
     4381 attcattaat aattgtaata gtatggcaaa tatatttcaa aaaatttata gtatgtgcga
     4441 tagctcatag tattgacaca atggtgggaa aaatccataa tatataatct agattacctg
     4501 gcaaataatg gaatactttt taaaaatgca tttgttacca cttcattgtg cagcccaagt
     4561 agagcaagtt ttttaacagg taaatatgca tcacgccatg gtgttataaa taatttttca
     4621 ccatggaaag ctgaaaatgt gacatttatg gaattgttaa agcaaggtgg atatgataca
     4681 gctttcattg gaaaatggca tatgcccggg aaaggtttac ctcatttacg gggtgttgat
     4741 ctttttattt cctttacaag aaaagatggg cagggtgatt attataattg tccacttttt
     4801 gttaatcata agccagtctc taataataaa gaatatatta ctgaagaatt aacagattat
     4861 gctcttgaat tcatttctca accaagaaaa aatccatttt gtctgtatct ttcgcataaa
     4921 gcagttcatc atgattggaa accgccacgg catcttaagg gattatataa aaatgaaaaa
     4981 attgatttcc ttgcaccaga atcagataag tggaacacat ggagtggaaa taactggctt
     5041 gaagggtcaa tgggctcaat gcatgatata tacaagcgat actgcgaatg cctggtttct
     5101 gttgatgaac aaattggtcg tattattcag ctactaaata aaatgggcat cttagaaaat
     5161 acggtaatag tatatgctgg cgataatgga tatatgtggg gcgaacatcg actttatgca
     5221 aaacactggc cttatgaaga atcaatcagg atcccgttta tttttttcta ccccggaatt
     5281 gtcaaaaaac ctggtaaaag ggagcaaatg attttgaata ttgatctggc tccaagttta
     5341 ttacagttag ctggtattcg tattccagat tggatgcagg gtgaaagttt tgtcccaatt
     5401 gtaaaaaacc cactttcacc gggaagaaag tcctttatgt atgaactttt taaagatttc
     5461 ccttttggag ggagggttcc tccaaacaag actctgcgta cagaacgtta taagtacata
     5521 gaatggcaag cttgtagagg acctgaacta tatgatttat tggaagatcc aaaagagatg
     5581 aataatatga ttggcacaca aaaagccaaa aaaatcgtgc ccatgttaaa gaaggaatta
     5641 gaagaaataa agataaaata tggtgccata gattgaattt tgtgagtaat aaatactgat
     5701 attttttata atttttattg acaattatat agttaatttt gtatggtcac aaaacgttga
     5761 tttaaaagac tacatcctgt taatactgaa gtgaggatgt actaaaaaaa atttttttta
     5821 aagaattttt ttatcaaaaa agcttgacag tacaatagtg tttaactata tgtacatatc
     5881 catatcgctt aacgatagaa gctgacgtgt tctttttaac catcgtataa aaaatttctt
     5941 aaagaaagct ttaaaaaaat cgaaaagttt gtaagaaaaa cttgacgaac taaaataaag
     6001 cgagtatatt taaagaaaag ctttcagaac actttaagca actctcccac atgagtggga
     6061 ttttttatcc aaaatcatgg ttctttgaaa
//
LOCUS       DAOSZP010000050         6079 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_10954_length_6079_cov_2.987384, whole genome shotgun sequence.
ACCESSION   DAOSZP010000050 DAOSZP010000000
VERSION     DAOSZP010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6079
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10954_length_6079_cov_2.987384"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1141)
                     /locus_tag="PK348_01910"
     CDS             complement(<1..1141)
                     /locus_tag="PK348_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020677484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HXK64997.1"
                     /translation="MQHHQKVLFIDLSTGYYRLARYPIGDFFGPVDLGLHIAQRYYTT
                     NIGVGVLAGSIFPGSNRLFVNGYSPCWNSFFISSMGGAGLVFDNLGINMIALSKKAPT
                     PSVLYLNRIHGEEIEIEIKPIDVFRIWESGHGGVYSLMHEVLSMYAGKYLTDPRVIAT
                     GPASHATDFGALCSAPVKDGTLTWVDTWAGRGGFGSKLFREHGIAAIIYGGTYIDEDF
                     RDRKVADEWFIDKYSKKLAAKDLEATTKYRFDPQFNTGGTFGVNFATIGGKLLAFNYR
                     SIYLTEEERLAIHQKLVVDHYLKQFNEETIATKSMKTCGEPCTAVCKKMRDIYKKDYE
                     PYQAMGPLCGVFDQRSAEMLVHKADMYGFDAISVGGVLSWLKECLD"
     gene            complement(1414..2430)
                     /locus_tag="PK348_01915"
     CDS             complement(1414..2430)
                     /locus_tag="PK348_01915"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HXK64998.1"
                     /translation="MPKTHVTIPFFIPHSGCRHRCVFCNQIYTSNTRFLPNEEYIVEK
                     IEQYLQTIKPGVNHIEVAFFGGSFTGLDEAIQQQYLAIAYTYLSLHKIDGIRLSTRPD
                     YINHKTLTLLREYGVTTVELGAQSMHDDVLQQAQRGHTVQDILNASKLIKEYGFNLII
                     QLMPGLPCDSFEKSIETARKTVLLYPSGVRIYPTVVIKDTQLETLYKENKFIPLSLDE
                     AIEVCTYMYDIFTSNNIPVIRMGLHPFDDAVADTIIAGPYHPSFGYLVKSRYLRNKLE
                     NNIKNFLQNNGNIQTLTIKLPQNKSAEYYGPHKENIAFLRNVFHTITLQFYSADVQDL
                     EIYG"
     gene            complement(2439..4292)
                     /locus_tag="PK348_01920"
     CDS             complement(2439..4292)
                     /locus_tag="PK348_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HXK64999.1"
                     /translation="MNALWIVTAAATFYMLAYRFYGAFLAAKVLALDETRVTPAYRLN
                     DNTNYYPTNKWVLFGHHFAAIAGAGPLIGPVLAAQFGYLPGFLWILIGSVVAGGVHDM
                     VILVASIRKDGKSLAEIAKEEIGPVTGLAALFAVLFILIVAIAGLGLVVVNSLYNNPW
                     GTFIIAMTIPIALLMGVYMKSIRPEKVLETSIIGIVLLLITVIYGRHILGTNIEGFFT
                     LNQHNLAITLAVYGFIASVLPVWLLLAPRDYLSSFMKIGVIVLLAIGVVAVHPDLHLP
                     ALTQFIHGNGPIIPGKLFPFVFITIACGAISGFHSLISSGTTPKMIENEKYALPIGYG
                     AMLTEGFVSMMALIAAAVLIPGDYLAINTHLSFDTLAQMGFPVDKINDLSKLVGIDLA
                     GRPGGAVSLAVGMAYIFSSIPFLSHLMAYLYQFAIMFEALFILTTIDAGTRVARYVVQ
                     ELGGYVYKPFGELRSLSGNIIASLLVVIAWGYFIYTGSVQTIWRMFGTANQLLAMLAL
                     CIGTTVIIKMNKIKYIWVTLIPMLFMLSTTFTAGITLIIEYIPKALSSQPDAYSFMIN
                     IFLLTLMILLALFILLDSVYKWYGFITGKRPIKNNTEQPSQYSKEFPKMSD"
     gene            complement(4650..5771)
                     /locus_tag="PK348_01925"
     CDS             complement(4650..5771)
                     /locus_tag="PK348_01925"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HXK65000.1"
                     /translation="MRSSLFYIIFTAILIIYLTANFYVLQRIQKLVPNQYKILTATFI
                     SILALSFLLGRILERYTVCAASDFLIWIGALWLGIFIYLFFGFIIGDSIQGIVHIFIK
                     TLNIQKAAYSIVIIVSIIITSVGFINARTPHVKEIAIHIDKPSSPTHLKIAYASDIHL
                     GSIIANSRLQNLVRLINSAQPDLIIFGGDILDEDIKPVIENNLGELLIQLKARYGVIA
                     VPGNHEYIGGIQNAINYIKDHNIRVLRDEVYSIGDIQIIGRDDRSKYAFTGSRRLPLE
                     NLIKQIDISKPVIVIDHQPFKLSQTASYPVDFLLSGHSHHGQLFPFNYITKKLYEISW
                     GYKVINKTNVYVSSGYGTWGPPIRTNSYPEIVIFNVYFK"
BASE COUNT         1915 a         1143 c         1190 g         1831 t
ORIGIN      
        1 gatcaaggca ttccttaagc catgatagta cgccaccaac tgaaatagca tcaaacccat
       61 acatatcagc tttgtgtaca agcatctcag ctgacctctg atcaaacacg ccgcacagtg
      121 gtcccattgc ctggtacggt tcgtaatctt ttttgtagat gtcacgcatc tttttacaca
      181 cggcagtaca aggctcaccg caggttttca tgcttttggt ggcgatagtt tcttcattaa
      241 attgcttcag gtaatgatca acaacaagct tttgatgaat agcaagcctt tcttcttcgg
      301 tcaaataaat tgagcgatag ttaaatgcaa ggagcttacc gccgatagta gcaaaattaa
      361 caccaaaggt accaccagtg ttaaactgcg ggtcaaagcg atatttggtt gtagcttcta
      421 agtctttagc tgccagcttt ttgctgtatt tatcaataaa ccattcatca gcgaccttgc
      481 ggtctctgaa gtcttcgtca atatatgtac caccgtatat tattgcggca atcccatgtt
      541 cgcggaaaag ctttgagcca aatccaccac gtccagccca ggtatcaacc caggtgagcg
      601 tgccatcctt aacgggtgcc gaacacagtg caccaaaatc tgttgcatgt gaagcagggc
      661 cagtggctat gacacgggga tcagttagat atttgccagc atacatggaa agtacttcat
      721 gcatcaggga atacacacca ccgtgtccgc tttcccatat cctgaataca tcaatgggtt
      781 taatttctat ttcaatttct tcaccatgaa tacggttaag atacagcacc gaaggtgttg
      841 gtgctttttt ggaaagggca atcatgttta tacccaggtt atcaaacacc aggcctgcgc
      901 cacccatgga tgaaataaaa aaactattcc agcagggtga atacccattg acaaagagtc
      961 tattagagcc ggggaagata gatccagcta atacacccac accaatattt gtggtgtagt
     1021 agcgctgtgc aatgtgcagc ccaagatcaa caggaccaaa aaaatcgcca atagggtatc
     1081 gtgctaagcg atagtaaccg gtggaaaggt ctatgaagag tactttttga tgatgttgca
     1141 tatttttctc ctgaaaatta ttttgttaaa tccaggcctt ttacctcatg ttcagtgatc
     1201 ctgattcata aacaatcatc ctgacgcaca aacggtcatc ctgaagcaca aacagtcttc
     1261 ctgaacttga ttcagtggct tctgcatact acgtcatact gaacttgatt catcatcttg
     1321 atgagagaga ttctgattca ggttcaaaat gactaaacca ttaagtaatg ctaaatcctg
     1381 aattacgttg tgattcagca ccactatctg aatttaccca tatatttcga gatcctgtac
     1441 atcagcagaa taaaattgca atgtaatagt gtgaaaaaca ttacgtaaaa aagcaatatt
     1501 ttctttatgt gggccataat attctgcgct tttgttctgg ggtaatttta tggtgagggt
     1561 ctgtatattg ccgttattct gtaaaaagtt tttaatgtta ttttcaagct tattgcgtaa
     1621 atatcgcgat tttactaaat aaccaaatga tggatgatat ggacccgcaa tgatggtatc
     1681 agctactgca tcatcaaaag gatgcaaacc cattcggatt actggaatat tgttgctggt
     1741 aaatatgtcg tacatgtaag tacatacttc aatagcttca tctaaggaca aaggaataaa
     1801 tttgttttct ttgtataatg tttctaattg agtatctttg ataacaacag tgggatagat
     1861 gcgtacaccg ctagggtata gtaaaacagt tttgcgtgct gtttcaattg atttttcaaa
     1921 agaatcgcat ggaagcccag gcataagctg aattataagg ttaaacccat attctttgat
     1981 gagctttgaa gcgtttagta tatcctggac tgtatggcct cgttgtgctt gctggagtac
     2041 atcatcatgc atggattgag cccctaattc aactgttgta actccatatt ctctgagcaa
     2101 agtaagagtt ttatggttta tatagtcagg acgggttgat aatcgtatgc catcaatttt
     2161 atgcagtgat aaatatgtat aagctatcgc taaatattgt tgttgtattg cttcatccag
     2221 gccggtaaag ctcccgccaa aaaaagcaac ttcaatatga tttacacctg gttttatggt
     2281 ttgtaaatac tgctcaattt tttcaacaat atattcttca ttgggaagaa atcgtgtatt
     2341 gctagtgtat atctggttgc agaacacaca tcggtgcctg caaccagaat gtggtataaa
     2401 gaaaggtatg gttacatgtg ttttgggcat gtaatcattt aatcactcat ttttggaaat
     2461 tcttttgaat actgtgatgg ctgttctgta ttgtttttga tgggtcgttt gccggtaata
     2521 aatccatacc atttatacac agaatccagt aaaataaaca aagctaacag aatcattaag
     2581 gttaataaaa aaatattaat cataaaactg tatgcatcag gctgtgatga aagtgctttt
     2641 ggtatatatt caattataag ggttataccc gcagtgaacg tggtgctgag cataaaaagc
     2701 atgggtatca gtgtaaccca gatgtactta attttattca ttttgatgat aaccgttgtg
     2761 ccaatacaca gagctaacat ggccaaaagc tgatttgctg tgccaaacat tcgccagatt
     2821 gtttgtaccg aacccgtgta gataaaataa ccccatgcaa taacaacaag caaactggca
     2881 ataatattac ctgataaaga tcgtaattcg ccaaatggtt tgtaaacata gccacccagt
     2941 tcctggacta cataccgtgc aactcgggta ccagcatcaa ttgtggttaa aataaatagc
     3001 gcctcaaaca ttatggcaaa ctgatacagg tatgccatca aatggcttaa aaaaggtatg
     3061 gatgaaaaga tatacgccat acccacagca agtgaaacag caccaccagg gcggccagct
     3121 aaatcaatgc caacaagttt tgacaggtca ttgattttgt ccaccgggaa tcccatctgt
     3181 gccagtgtat caaatgacag gtgagtgttg attgcaaggt aatcaccggg aataagcact
     3241 gcagcagcaa tgagagccat cattgataca aacccttcag taagcatggc accgtagcca
     3301 atgggtaacg catacttttc gttttcaatc atcttgggtg ttgtaccgga tgagatgagt
     3361 gagtggaatc ctgaaatagc accacaagct atcgttataa atacaaatgg gaatagtttt
     3421 cccggaatga ttggaccatt gccgtgaata aattgtgtca atgccggtaa atgtaaatcc
     3481 ggatggacag caaccacgcc aatagcaagc agcactataa cgccaatttt cataaatgat
     3541 gacaggtaat cacggggggc aaggaggagc catactggca atacgcttgc tataaagcca
     3601 tagacagcaa gagttatcgc cagattatgc tgatttaaag taaagaatcc ttcaatgttt
     3661 gttccaagaa tatgcctgcc ataaataacg gttatcagta ataacacaat cccaataatt
     3721 gatgtttcaa gaaccttttc ggggcgtatg gatttcatgt atacacccat aagcagtgca
     3781 atggggatgg tcattgctat gatgaaagtg ccccatggat tattatataa agaatttact
     3841 accacaaggc caagtcctgc gatagcaact attaaaataa acaatactgc aaataaagcc
     3901 gcaagccctg tcactggccc aatttcctct ttagcaatct ctgccagcga tttgccatct
     3961 ttgcggattg atgcaacaag tatcaccatg tcatgcacgc caccagcaac tactgagcct
     4021 ataaggatcc acaaaaaacc cgggagatag ccaaactgcg cggcaagaac agggccaatt
     4081 aatgggcctg cacctgctat agcagcaaaa tgatgaccaa atagtaccca tttattggtg
     4141 ggatagtaat tggtattatc gttcagtcta tatgctggag taacacgtgt ttcatcaagt
     4201 gccagcacct ttgcagctaa aaaagcacca taaaagcgat atgccagcat gtagaatgtg
     4261 gcggctgcag taactatcca taatgcgtta attttttcct gagggttgta aatctgtgtg
     4321 actatagcaa aggatagtgc aaataataat gatattacaa tccagaaggc aatttttaaa
     4381 agcttcttca tagtatatct cctgatgaag aaaattttat gtgctaacgg tagacacctg
     4441 ttctcacgtt gtgtcaagag attttagtaa aaaaatatgt attgcactaa taattggtgg
     4501 tcatgctttt aatagcaaag taatccagta ttcatcctta ctataatagg ttgggagttt
     4561 tcatgtattc cttctttaaa aaattttaat gaataaatac tataagcaaa atgaaattag
     4621 gcttaccgca gtgatagagt ggtaaatatt tatttaaaat atacattaaa tataactatc
     4681 tcagggtagc tattggtacg tatgggaggc ccccatgtac cgtaaccact tgatacatat
     4741 acgttcgttt tgtttatgac tttgtatccc cagcttattt cgtaaagctt ttttgtgata
     4801 tagttaaagg gaaataattg accatgatgt gagtggccgg aaagaagaaa gtctacagga
     4861 tatgatgctg tttgtgatag tttaaatggc tgatggtcaa tgacaataac tggttttgaa
     4921 atatctatct gttttattaa attttctaat ggtaaacgcc tggaaccggt aaatgcatat
     4981 ttgcttctgt catcgcgtcc gattatctgg atatcgccaa tggaatatac ttcatcccgt
     5041 aacacccgta tattatgatc tttgatataa ttgatagcat tctgtatccc cccaatatat
     5101 tcatggtttc cgggtactgc aattacgccg tatctggctt taagttgtat cagtaactcg
     5161 cctaaattat tttcaattac gggtttgata tcttcatcca gtatatcacc accaaaaatg
     5221 atgagatctg gttgtgcgct attaataagc cttaccaggt tttgcaaacg agagtttgct
     5281 atgattgatc ccagatggat atcgctggca tacgcaattt tcagatgcgt tggtgatgaa
     5341 ggcttatcaa tatgaatggc tatttccttt acgtgagggg tgcgcgcgtt aataaatccc
     5401 acagatgtga ttattatact tacgattatg actatactgt aggctgcctt ttgaatatta
     5461 agtgttttta taaagatatg aactattccc tggatactat cgccaataat aaacccaaaa
     5521 aataaatata tgaaaatacc caaccagagt gctccaatcc agatgaggaa gtcactggct
     5581 gcacacactg tatagcgttc aagtattcgg ccaagcagaa acgaaagagc aaggatgctt
     5641 ataaacgttg ctgtcaaaat tttatactga ttgggcacca gcttttgtat acgttgcagc
     5701 acgtaaaaat tggctgtaag atagattatc agaattgcgg tgaagatgat ataaaacagt
     5761 gaacttcgca taaagttaat tccgtaataa tggtatatgc taatattagc acaggttata
     5821 tatttagtat aaaattaaaa gcctaaaata gtaataaaat atttttttgt tattgacttc
     5881 actatttatt tgaaatacaa taaatagtga aagggtatgt aaatcataac attggggtta
     5941 ttattatgaa acacaaaaat ataaatgtta ctttatgtat tattgtgctg atcctttgca
     6001 tttattgcac atatcttttt tccccgcaaa tgtttaaaag taatgaattt attcaaacag
     6061 atatatctcc agacaggtt
//
LOCUS       DAOSZP010000051         6078 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_10956_length_6078_cov_3.059937, whole genome shotgun sequence.
ACCESSION   DAOSZP010000051 DAOSZP010000000
VERSION     DAOSZP010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6078
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10956_length_6078_cov_3.059937"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..470)
                     /locus_tag="PK348_01930"
     CDS             complement(<1..470)
                     /locus_tag="PK348_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65001.1"
                     /translation="MRLFRQLIIQGINIIFLSLLLLLGWMLHTVKIEGNLLVIFIVFS
                     IVTIFPLYTYYIERFYKNIIPLKYEDLYIQTVDMFLSLQSFDDIIKVIFDKVLQYIGV
                     KSGLLIFYSHASDDYTIYYQKQQKQKVIRKAHIDKNNIIFKIIQSGDDILIKSK"
     gene            complement(482..1093)
                     /gene="tmk"
                     /locus_tag="PK348_01935"
     CDS             complement(482..1093)
                     /gene="tmk"
                     /locus_tag="PK348_01935"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HXK65002.1"
                     /translation="MKIKTPLFIVFEGIDGAGKSTQANLLFSFCRAKTPAVLLQEPTD
                     SQYGKLLRKMLKGEIPGTRDELLDLFIKDRAYDVEHNITPSLNRGYVVIVDRYFYSTA
                     AYQAGDGIKSSDIVRMNIERGFPVPDRVYFIDIEPAIALERIHRRSGDNKEIFETLQT
                     LETIRKNYLSIADSTFAVIQGSLGVEDMFQEVLKDFEKHFVTG"
     gene            complement(1107..2498)
                     /locus_tag="PK348_01940"
     CDS             complement(1107..2498)
                     /locus_tag="PK348_01940"
                     /inference="COORDINATES: protein motif:HMM:NF012562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HXK65003.1"
                     /translation="MIQFDFNYFMDEFIGSEHGIAKKDIDDLTPQCDAARKRMDDEKA
                     KGMLGFLELPFADTIVEDIRAFKATVPWCDTVALIGIGGSALGPQALKNALADIYLNE
                     LPDEIRGKRCKAYFFDNVDPGEMQSLLKILNIPSTLFLVISKSGGTTETNANFAILLD
                     AIKKQTKDYKKHIVTITDPDKGILRQITQEEGFVSFPVPSNVGGRFSVLSAVGLVIAE
                     FLGIDAYGLLKGAARVVDRYYGKTVWENAPLLNAAFHYLFDIKKNKRINVLMPYTRKL
                     YLFADWYRQLWAESLGKRFDIQGKEVMAGLTPVAALGTIDQHSQLQLYLEGPNDKVIT
                     FLKLKDFGTDILMPQFYKGKQELEYLSGKSLRKLNEFEEQATELVLKDASRPNASIIL
                     PKLDEEAVGQLIMFLEMQTAYAGYLYNINPYDQPAVEQGKRFTFGLLERPGYEDYKEK
                     FVKGYVKKESYIL"
     gene            complement(2621..3577)
                     /locus_tag="PK348_01945"
     CDS             complement(2621..3577)
                     /locus_tag="PK348_01945"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013100168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HXK65004.1"
                     /translation="MIYPATEIYIQRAAQAIKQGNVVAFPTETVYGLGANALNAEAVA
                     KIFEIKQRPYFDPLIVHVDSREMVQAVVATLPDIALRCMEAFWPGPLTLVLPKSTIIP
                     EIVTAGLDTVAVRMPQHQVAQKLIQASGVPIAAPSANPFGYLSPTTANHVASQLGDKV
                     EIILDGGQCPVGVESTIIKIEKDSITLLRHGGIPVEDIEAVAGKISVIRHTQVEAPGQ
                     LPYHYAPKTKIVLINDISKIPDDSSCGVILYKKRDLPFKNIHVMYLSDNGDLRQAAAN
                     LFTCLHALDAMRLSVIYAEAIPEVGLGQAIMDRLKKAQARHS"
     gene            complement(3578..4033)
                     /locus_tag="PK348_01950"
     CDS             complement(3578..4033)
                     /locus_tag="PK348_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010299248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiD C-terminal domain-containing protein"
                     /protein_id="HXK65005.1"
                     /translation="MTKRQRILRKLVNLYPPFLGAGIRIKFDDTTNKVAVSMKLTWYN
                     RNYVGTHFGGSLYTMCDPFYMILLMDILGKEYIVWDKSAKIEFVKPGRSTVHAEFYIP
                     DEIVEKIKKEVDKNGKSMPEFVAEVKDEQGEVVARVYKYIYVRKKDTGR"
     gene            4229..4687
                     /locus_tag="PK348_01955"
     CDS             4229..4687
                     /locus_tag="PK348_01955"
                     /inference="COORDINATES: protein motif:HMM:NF024560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3996 domain-containing protein"
                     /protein_id="HXK65006.1"
                     /translation="MKNILLPCIFVLMFATIASAAHNFGAGFILGDPSGLTAKIFMGK
                     SDAIDIGIGESADDLYIYADYLRHFHGVFPINELVFYLGVGAGFHDWEKDRKNDHDEE
                     NRIDVRIPVGLEYTFTKVPVGIFIELVPALRIIPDVDFDIRGGLGARYYF"
     gene            complement(4788..>6078)
                     /locus_tag="PK348_01960"
     CDS             complement(4788..>6078)
                     /locus_tag="PK348_01960"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013826098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HXK65007.1"
                     /translation="GTCFAVMGNTVICVDTDSDKIEKLKKGIIPIYEPGLDELVVENH
                     KKGNLLFTTDIKEALEKTNVMFIAVGTPMGEDGSADLRHVLDVAQSIGKFMTHDMIVV
                     DKSTVPVGTAEKVKATIQEELKKRGVTFKVMVVSNPEFLKEGAAVEDFMRPDRVIIGT
                     DDEEALNVMKELYAPFTHSHERFIAMDIRSAEMTKYAANAMLATKISFMNEIANICEK
                     VGADVNMVRIGIGSDHRIGYSFIYPGVGYGGSCFPKDVQALIKTSRDYGYEPKIITAV
                     EEVNAKQKLSLVDKVVKRFGQDLSGKVFAIWGLAFKPETDDMREAASITIIKELLKRN
                     AKVKAYDPKATEQAQGFYLKGLQNIEYCDSKYDALTGADALLLVTEWKEFRSPDFDEV
                     KKRLKNPVIFDGRNQYNMKKLQKDGFEIYQIGVGKVK"
BASE COUNT         1775 a         1352 c         1004 g         1947 t
ORIGIN      
        1 attttgcttt ttatcagaat atcatcccct gattgaatta tcttgaatat aatattattt
       61 ttatcaatat gggctttacg tattactttc tgtttttgct gtttctgata gtaaatggtg
      121 taatcatcgc ttgcatggga ataaaatatt agcagccccg acttaacacc aatatactgt
      181 aataccttat caaagataac cttgataata tcatcaaatg actgcaacga caaaaacatg
      241 tcaactgtct gtatatagag atcttcatat tttaatggaa ttatgttttt ataaaatcgt
      301 tcaatataat acgtatacag aggaaatatg gttacgatgg aaaaaactat aaatattaca
      361 agaagattac cttcaatctt cacagtgtgt agcatccacc ccaaaagcag caaaagggaa
      421 agaaaaataa tattaatccc ctgaataatt aactggcgaa atagcctcat atactatcta
      481 tctatcctgt cacaaaatgt ttttcaaaat ccttcaaaac ctcctgaaac atatcttcca
      541 ctcccagact gccctgtatg acagcgaagg tggagtctgc tattgacaaa taattttttc
      601 tgatagtttc aagagtttgc aatgtttcaa atatttcttt attgtcacct gaccgcctgt
      661 gtattcgctc taaggcgata gcaggctcaa tatcaatgaa atacacccta tcaggcacag
      721 gaaatcctct ttcaatattc atcctgacaa tgtctgaaga tttgatacca tcacctgcct
      781 gataggcagc agttgaataa aaatatctat caactatcac cacataccct ctgttaagcg
      841 atggcgttat gttatgttcc acatcatatg ccctatcctt tataaaaaga tcaagaagct
      901 catcccttgt accagggatt tctcctttta gcattttcct aagaagcttg ccatactggc
      961 tgtcggttgg ctcctgcaaa agaaccgcgg gagtttttgc cctgcaaaaa gaaaacaaaa
     1021 gattggcctg cgttgatttg cctgcaccat ctattccttc aaagacaata aaaagcgggg
     1081 ttttaatttt catgattgtg atttttttat agtatatatg attctttttt tacataccct
     1141 tttacaaact tttctttata gtcctcatac cccggtcgtt ccagcagccc aaacgtaaaa
     1201 cgtttcccct gttccaccgc aggttgatcg tatggattta tgttatacag ataaccagcg
     1261 tatgctgttt gcatttccag aaacatgatt aactgaccaa cagcctcttc atcaagtttg
     1321 gggagtatga tcgatgcatt aggccggcta gcatctttca aaacaagctc agttgcctgt
     1381 tcttcaaatt cattcagctt ccgcaaactt ttcccgctaa ggtattccag ttcctgtttg
     1441 cctttgtaaa actgcggcat cagtatgtcg gttccaaaat ccttcagttt aagaaatgtt
     1501 ataactttgt catttggccc ttccagataa agctgtagct gcgaatgctg atcaatagta
     1561 cctaaagcgg caaccggcgt taggcctgcc atcacctctt ttccctgtat atcaaaacgc
     1621 tttcccaacg actccgccca taactgacga taccagtcag caaacaggta cagcttgcgc
     1681 gtatagggca tgagcacatt aatacgttta ttctttttta tatcaaatag gtaatgaaat
     1741 gctgcattta atagcggagc gttttcccac acagtcttac cataataacg atctacaaca
     1801 cgtgcagcac ctttgagcaa cccatacgca tcaattccca gaaattcagc aatcaccagg
     1861 cccaccgctg aaagcaccga aaatcttcca ccaacatttg atggaacagg aaatgacaca
     1921 aacccctctt cctgtgttat ctgtcgtaat atgcctttgt cgggatcggt tatagtcact
     1981 atgtgttttt tgtagtcctt ggtttgtttt tttattgcat ccagtaaaat tgcaaagttt
     2041 gcattggttt cagtagtccc tcccgatttt gatatgacca aaaacaacgt tgaaggaata
     2101 ttgagtattt ttagtaacga ttgcatctca ccaggatcaa cattatcaaa aaaatatgct
     2161 ttacatcgtt tacccctgat ttcatcaggc aattcattaa gataaatatc cgccagggca
     2221 tttttcaatg cctgtggccc caacgctgat ccaccaatac caattaaagc tacggtatca
     2281 caccagggca ctgttgcttt aaatgcacgt atatcctcaa caatagtatc agcaaatggc
     2341 aattccagaa aacccagcat cccctttgcc ttttcatcat ccattcgctt acgtgcagca
     2401 tcacattgtg gcgtaagatc atctatatcc ttctttgcaa taccatgctc ggatccaata
     2461 aactcatcca taaaataatt aaaatcaaac tgaatcatgc aaccactcct gtaaaaagta
     2521 tactttttat atactacaaa atcatctcat gtcaaccata ttattggcga tagttactga
     2581 taaaaaagaa tgttgctttt aaaaggaatg ttatacgtaa tcagctgtga cgtgcttgcg
     2641 ctttttttaa tctatccatg atggcctgcc ccagaccaac ctcaggaatt gcctccgcat
     2701 atatgacaga aagcctcatt gcatcaagag catgaaggca tgtaaaaaga tttgctgcag
     2761 cctgccgcaa atcaccatta tcagaaagat acatgacatg gatattttta aatggcagat
     2821 cccttttttt ataaagaata actccacagg aactgtcatc agggattttg cttatatcgt
     2881 ttatcaggac aatttttgtt tttggagcat agtgataggg caattgacca ggagcttcaa
     2941 cctgtgtgtg acgtataaca gatattttac ctgcaacagc ttcaatatct tcaacgggta
     3001 tccccccatg acgaagcaat gtaatgctat ctttttctat cttgataatg gttgattcaa
     3061 ccccaacagg gcactgtcca ccatccagaa tgatttcaac cttatcaccc agctgcgatg
     3121 caacatgatt cgcagtagtg gggctcaaat atccaaatgg gtttgcactt ggcgcagcta
     3181 tgggcacgcc acttgcctgt atcagttttt gtgcaacctg atgttgtggc attcttacag
     3241 caacggtatc aagtcctgca gtaactatct ccggtattat tgtgcttttt ggcagtacca
     3301 gtgtcaatgg accaggccag aatgcttcca tacatcgcag ggcaatatcc ggtagagtag
     3361 ctaccaccgc ctgcaccatc tcgcgcgaat ccacatgaac aataagagga tcaaaataag
     3421 gtctttgctt aatttcaaaa atctttgcca ctgcttctgc atttaaagca tttgcaccaa
     3481 gaccatatac cgtttcagtt gggaatgcca ctacattgcc ctgcttaatt gcctgtgctg
     3541 ccctctggat atatatttcg gttgcaggat atatcattta tctaccggta tcctttttcc
     3601 tgacataaat atatttatac acacgagcaa ccacctcacc ctgttcatct ttaacttcgg
     3661 caacaaattc tggcatagac ttaccgtttt tatcaacttc cttttttatc ttttctacaa
     3721 tttcatcagg tatataaaat tcagcatgga ctgttgagcg cccgggtttt acaaattcaa
     3781 tttttgctga cttatcccat acaatatact ctttcccaag gatatccatc aataaaatca
     3841 tataaaatgg gtcgcacatg gtatacagcg agccaccaaa atgggtcccc acgtaattac
     3901 ggttatacca tgtaagtttc attgaaaccg caaccttatt tgttgtgtca tcaaatttta
     3961 ttcgtatacc ggcacctaaa aatggtggat aaaggttaac aagttttctc agtattcgtt
     4021 gtcgttttgt catactcttt ttacatctta ataataaagt gtgtttagct cacctactat
     4081 gaatagtatt atataacata taatgaatca tactaaaaat tttatggaca cgcagtatga
     4141 ttaaaaaatt gaattgacgt acctgttgaa ttatactgta taataatttt accttacata
     4201 accttatcaa tgccattgag gtgaacccat gaaaaacatt ttgttgccat gcatttttgt
     4261 attgatgttt gcaactatag catctgctgc acataacttt ggtgctggct tcattctggg
     4321 tgacccatca gggttaacag caaaaatttt tatggggaaa agtgatgcca tagatattgg
     4381 cataggagaa tctgcagatg atttatatat ttatgcagat tatttacgcc atttccatgg
     4441 tgttttccca attaacgaac tggtgtttta ccttggtgtt ggtgcaggat tccatgattg
     4501 ggaaaaagac aggaaaaatg accatgatga agaaaacaga attgatgttc gtatcccggt
     4561 agggcttgaa tatacattta caaaagttcc agttggcata tttattgaac ttgtacctgc
     4621 actacgtatt attcccgatg ttgattttga tattcgcgga ggcttaggag cacgatatta
     4681 tttttaatag tacgatagtt cacgcagagt attgattgaa taccacaagg gagataaagc
     4741 tatcctctac ctgtatactc tgcgtgatgg taccttaatc atataaccta tttcactttg
     4801 cccacaccaa tttgataaat ttcgaagcca tctttttgca attttttcat attatactgg
     4861 tttctgccat caaagataac cggatttttc aatcgctttt tgacctcatc aaaatccggg
     4921 cttctgaatt ccttccactc agtaaccagc agcaacgcat ccgcaccagt gagtgcatca
     4981 tatttactat cgcaatactc aatgttctgt aatcctttaa gataaaatcc ttgtgcctgt
     5041 tctgttgcct tgggatcata ggctttaact tttgcatttc ttttcaataa ctcttttata
     5101 atggtgatac ttgctgcttc acgcatatca tcagtttccg gcttgaaagc aagcccccag
     5161 attgcaaaaa ccttacctga cagatcctgg ccaaagcgtt ttacaacctt atccaccagt
     5221 gaaagtttct gttttgcatt tacttcctca actgcagtga ttatctttgg ttcatatcca
     5281 taatcccttg aagtctttat cagcgcctgc acatccttgg ggaagcatga accaccatac
     5341 ccaacaccgg gataaatgaa gctgtagcca attctatgat cactgccaat gccaatgcgc
     5401 accatattaa cgtctgcacc tactttttca caaatattgg ctatttcatt cataaagctt
     5461 atctttgttg ccagcatggc gtttgcagca tactttgtca tttctgcact tcgtatatcc
     5521 attgctataa agcgctcatg gctgtgtgta aatggtgcat ataattcttt cataacattc
     5581 agtgcttctt catcatcagt tcctatgata accctgtcag gacgcataaa atcttcaacc
     5641 gcagcacctt ctttcaaaaa ctctggattg ctcactacca taaccttgaa ggtaacacca
     5701 cgctttttca gttcttcctg aatggttgct ttaacttttt cagcagtgcc aaccggtact
     5761 gttgatttat ccacaacaat catatcatgg gtcataaatt taccaattga ctgtgctaca
     5821 tcaagcacat ggcgcaaatc agcactgcca tcctcaccca tgggcgtgcc aacagcaatg
     5881 aacataacat ttgttttttc cagtgcttct ttaatatcag tagtaaaaag tagatttcct
     5941 tttttatgat tttctacaac cagctcatca agccctggct cataaattgg gataattcct
     6001 tttttaagtt tttcaatttt atcactgtca gtatccacac aaattaccgt atttcccatt
     6061 actgcaaaac aagttccg
//
LOCUS       DAOSZP010000052         6003 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_11123_length_6003_cov_3.565232, whole genome shotgun sequence.
ACCESSION   DAOSZP010000052 DAOSZP010000000
VERSION     DAOSZP010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6003
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11123_length_6003_cov_3.565232"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..723)
                     /locus_tag="PK348_01965"
     CDS             complement(<1..723)
                     /locus_tag="PK348_01965"
                     /inference="COORDINATES: protein motif:HMM:NF036774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgO family outer membrane protein"
                     /protein_id="HXK65008.1"
                     /translation="MKYTKQTTFIVIIIMAIALSVSAIPGKKVIGVTAFTNLSKDTTF
                     DWLCIGIADTISYKLRNVQEYILVDRVNVDKLIGELQLSQSGLIDENTVEAGKALGAN
                     ILVVGNFQKFGDQIRITAKIVDVQSHAVINQVQVTGSLNDIFNLQDSLALGIIEKNDT
                     AITTETKTKIVQNDTNNISAYEYFVRGQKFLLYQLDYSKAIEMYQKAISIDPNYAIAY
                     AGLGKAYSLRSWELREYHNTTDP"
     gene            complement(1041..3476)
                     /locus_tag="PK348_01970"
     CDS             complement(1041..3476)
                     /locus_tag="PK348_01970"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HXK65009.1"
                     /translation="MSNRVTEILCVILWGFAVIVFLSLATFHIADYDTLGTMPVQNIL
                     GPAGVILAQILRISFGIASYFVVAILLLTGWGLVKYKSLEPVLEHIFAITFAMVSTSL
                     LVTIIQPETIAPFAGGVVGMALYHVFTSVMGLTGAWIATILLVLISLILLGIVSLSYF
                     FDSQRFTSLANVAALLYRKIVLFKHRDSIPLIDITSFNAEKKIPWITRTKITLYKKTP
                     VRKPKLIEYDNILTYKGDSSYYNKKDHTDDLQIINTHTTPTENSILDNSDSSSMNTAD
                     TESKEATSQVVAVTNAVIELPQVDSIQDEHEPTVITKEISDIDEHYDEDDEYDDATDN
                     EEDMVARNVFEEIPINQKYSIPVDFLKQYTVHDETGYKQEIAKNSELLVNTLRDFGIE
                     SKVVNVNRGPVITLYELQIAPGIKVNRIVGLADDIAMALAASRVRVVAPIPGKKAIGV
                     EIPNKVRETVTLKDIIASSEYTHQQGRLKVGLGKDIMGKPVVIDIKKLPHLLIAGATG
                     AGKSVCVHSIITSLVYNYDPNYVRFIMVDPKLVELQIYNGIPHLLTPVIIEPFMVPKA
                     LKWTMMEMERRYYLLSEYNTRDIDRYNQMVERNKNGEYLPYIVIIMDELADLMMVAAK
                     EIEGYITRIAQKARAVGIHLVLATQRPSVDIITGIIKANFPARIAFQVAQKTDSRTII
                     DQNGAEKLLGKGDMLYQSPMSSFPVRIQGAFITEDELTSIVLYLKKIGRPRYVDIEQS
                     LVEFEDDDEEGEDELFIEAVKIVETTKKASASYLQRRLSIGYNRAARIIERMEELGYI
                     GPQQGSKPREVYI"
     gene            complement(3506..4336)
                     /locus_tag="PK348_01975"
     CDS             complement(3506..4336)
                     /locus_tag="PK348_01975"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HXK65010.1"
                     /translation="MAYSIVIFCILGIIQGVTEFLPVSSSGHLALLEAIPQVHTIFGA
                     MDTGAKLFFNVALHLASLAAIIIFYYNDIIKLVTGTIAEITKKHYGTHCQFACNIIVA
                     SIPAAMVGLFLNDVVETSISSPALVAGLLIINGIMLIVSNKIPKKSRKCEEMGILQAL
                     LIGLFQAVAILPGISRSGSTIVGGLLIGLKPEEAGKFSFLMAIPVIAGAGLLELRKLG
                     TINAFEFGIPVVIAMAITFIAALASLKLLVAMLKSLHLHYFGYYTIIAGVVALIILLV
                     "
     gene            complement(4338..>6003)
                     /locus_tag="PK348_01980"
     CDS             complement(4338..>6003)
                     /locus_tag="PK348_01980"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HXK65011.1"
                     /translation="LWTHRLMKEKKIRIIPIGGLHAIGGNMMAIEYDNEMIIIDCGIT
                     FPNGETPGIDFMIPDFSFILENKTKVKGIIITHGHEDHIGAIPFLLQKINVPVYATKL
                     TLGLVTSRLAERPVNAPTFIEIEPRDVIHIGSFVIEFLRVNHSIIDGVGLAIKTPIGT
                     IIHTGDYKIDYSPIDGNVADLHRFAEYGQQGVLLLISDSTNATNPGYTPSEIVLKKKL
                     LEIFAKSKGRIFVASFASNIHRIQQVLEASQKYNRKVVISGTTMQKNIEIATELGYLK
                     YKKDLIIDAKDIGKYTHKKLVVLCTGSQGEPMSALSRMATGTHKHFRIGAGDTVIITA
                     SVIPGNERMVYSVINSLMQMGADVFYEQDEEIHVSGHASQEELKLMITLTQPKYFLPV
                     HGEYRHLKAHASLAESLNIKPQNIIVAQNGDIIELTPNRFEKKGNIPLSTVYVDGSYI
                     GDITDDIIAERKTLASDGVIVISIVIGDGLLLYPPVIIARGFIGIKNDRFMQVLQKEL
                     SNTIEKQLLQNASPEHLTVAVKKLAKMIAHRFTNNTPLIETIIQEI"
BASE COUNT         1703 a         1243 c         1210 g         1847 t
ORIGIN      
        1 agggtcggtg gtattatggt attctctgag ttcccatgaa cgcagtgaat acgctttccc
       61 cagtcctgcg tatgctattg catagttggg atctattgat attgctttct gatacatttc
      121 aatagctttg gaataatcta attgatacag taaaaacttt tgcccacgaa caaagtattc
      181 atatgcactt atattattgg tatcgttctg tacaattttt gtttttgttt cggtggttat
      241 ggcagtatca ttcttttcaa tgattcccag tgccagcgaa tcctgcaggt tgaatatatc
      301 attcaatgat ccggtaacct gaacctgatt gatgacggcg tgtgattgta catcaactat
      361 ttttgctgta atgcgtatct ggtcaccaaa tttctggaaa ttgccaacaa caagaatatt
      421 ggcacccaat gctttgccgg cttcaacggt gttttcatca atgagaccgg attgcgatag
      481 ctgtaattcg ccaatgagtt tatcaacatt gaccctatcc accagtatat attcctgcac
      541 gttacgcagt ttataggata tggtatcagc aataccaata cacagccagt caaacgttgt
      601 atcttttgac agattggtaa atgcagtaac acctattact ttcttgccag gtattgcact
      661 gacagaaaga gctattgcca tgataataat gacaataaaa gtggtttgtt tggtatattt
      721 catagagcgc ctcacatatg atttaatgaa catcttgcaa ctacttcgta aaaaaatata
      781 tctttacctc actcacccct tccttcaaaa acctctctct gaacttgaag aaaggctcag
      841 taactatctg tttataagaa agaactatta agggtaatgg taaaaatatt ttcatctctg
      901 aaaagaatta ttctaatgca tagatcgcaa gcacatatac atgtaaatac atccaataat
      961 atgcacatgc tatcactttc aatagtggat agtataaaga tataataaaa tatgggataa
     1021 aaaaagtcac ttaaattttt ttatatatac acttcgcgcg gtttacttcc ctgctgcggg
     1081 ccaatgtatc ctaattcttc catgcgctca atgatacgtg ctgcgcggtt gtagccaatt
     1141 gaaagcctgc gctgaaggta tgatgctgag gctttttttg tggtttcaac tatctttact
     1201 gcttctatga aaagttcatc ttcaccttct tcatcatcat cttcaaattc caccaggctt
     1261 tgttcaatat caacataccg tggcctgcca atttttttaa ggtataaaac tatagaagtc
     1321 agttcatcct cggttataaa tgcgccctga atccgaaccg ggaaagaact cattggagac
     1381 tgatacagca tatcgccttt gcctaaaagt ttctcagccc cattctggtc aataatagta
     1441 cgtgagtcag ttttctgagc aacctgaaat gcaatacggg cagggaagtt tgccttgatg
     1501 atgccggtaa tgatatctac cgatgggcgc tgggttgcca gtaccagatg gatgcccact
     1561 gcacgtgctt tctgtgcaat acgggttatg tagccttcta tttcttttgc ggcaaccatc
     1621 atgaggtcgg caagctcatc catgatgatg acaatatagg gcaggtattc gccattcttg
     1681 ttgcgttcaa ccatctggtt atagcggtca atgtcgcgtg tgttatattc tgaaaggagg
     1741 tagtagcgcc gttccatctc catcatggtc cacttcagcg cttttggtac catgaatggc
     1801 tcaataatga ccggggtcag tagatgtggg atgccattgt atatctgcag ttcaacaagt
     1861 tttggatcaa ccatgataaa gcgcacatag ttgggatcat agttgtacac caggcttgtt
     1921 atgatggaat gaacacatac agatttaccg gcacctgttg caccagcaat aagcaggtgt
     1981 ggtagttttt taatatcaat aaccacaggt ttgcccatga tatccttgcc cagacctact
     2041 ttcaggcgcc cctgctgatg ggtatactca gaagaggcaa tgatgtcttt taatgttact
     2101 gtctctcgca ctttgttggg tatctctacg ccaatggcct ttttgccagg gattggtgct
     2161 accactcgaa cacgggaagc ggcaagtgcc atggcaatgt cgtcagcaag cccaacaata
     2221 cggttgactt taatacccgg agctatctgc aattcataca atgtaataac cggtccacgg
     2281 ttaacattga caacttttga ttcaatgcca aagtctcgca atgtgttgac cagaagttca
     2341 gaattttttg ctatctcctg tttatagcct gtttcatcat gcacggtgta ttgctttaaa
     2401 aagtctacag gtatgctgta cttctgattg attggtattt cctcaaaaac gttacgggca
     2461 accatgtctt cttcattatc tgttgcatca tcatattcat catcttcatc ataatgttca
     2521 tcaatgtcac tgatttcctt tgtaatcact gtgggctcat gttcatcctg tatactgtct
     2581 acctgtggca gttcaatcac cgcattggtt acggcaacaa cctgtgaggt agcttctttg
     2641 ctttcggtat cagcggtatt catggaagaa gaatctgaat tatcaagtat gctattttca
     2701 gtaggtgtag tatgtgtatt gattatctgc aggtcatctg tatggtcttt tttattataa
     2761 taggaactat cgcccttata cgtgagtatg ttatcatatt caatgagctt tggtttgcgc
     2821 actggtgttt ttttatacag ggtaattttg gtccttgtga tccagggtat cttcttttcg
     2881 gcattgaatg atgtaatgtc aataagaggt atggaatccc tgtgcttaaa aagcacaatc
     2941 tttctataca gcaacgcagc aacgtttgct aaagaggtaa accgctggct atcaaagaaa
     3001 tagcttaatg acactatgcc aagcagtata agtgaaataa gtaccagaag tattgtggcg
     3061 atccatgcac cagtcaaacc cattactgaa gtaaacacgt ggtataatgc catgccaacc
     3121 actccacctg caaaaggtgc aatagtttcc ggttgtataa tggttacaag aagtgaagtc
     3181 gaaaccattg caaaggttat ggcaaagata tgttccagga caggttctaa cgatttatat
     3241 ttgacaagcc cccatccggt taaaagcagt attgcaacaa caaaatatga tgctatgcca
     3301 aacgatattc tgagtatctg agcaagtatc actccggcag gtccaagtat gttttgtact
     3361 ggcatggtgc ccagagtatc ataatcagct atgtggaatg ttgccagtga aagaaagaca
     3421 ataacagcaa acccccacag tataacacac agtatttcag tgacacgatt ggacatacac
     3481 gtttgctaat cctgtacctg taattttata ccagtaaaat aattaatgct accacccccg
     3541 ctataatggt atagtaccca aaatagtgca ggtgcagcga cttgagcatt gccacaagca
     3601 gcttcaatga ggcaagtgct gcaataaatg ttatagccat agcaatcaca acgggaatac
     3661 caaattcaaa ggcattaatg gtgcccaatt tccgcaattc aagcagtcct gccccggcaa
     3721 taaccggtat tgccattaaa aaagaaaatt ttccagcttc ttcaggcttt aatcctatta
     3781 aaagacctcc aacaatggtg ctacctgagc gtgatatgcc cggcaaaatt gcaactgcct
     3841 ggaaaagccc aataagcaat gcctgcagta ttcccatctc ttcacatttt cggctttttt
     3901 tgggtatttt attagatact atgagcatga taccattaat tatcagaaga cctgcaacaa
     3961 gtgcagggct ggaaattgat gtttcaacaa cgtcgttgag aaacaaacca accatcgcag
     4021 caggtattga ggcaactatg atattgcagg caaactggca atgtgtacca tagtgttttt
     4081 ttgtaatttc ggcgatagtt ccggtaacaa gcttaatgat gtcattatag taaaatatga
     4141 ttattgctgc caggcttgcc aggtgcagtg caacgttaaa aaaaagtttg gcgcccgtat
     4201 ccattgcacc aaagatagtg tgaacctgtg gtattgcctc taacagtgca aggtgccctg
     4261 atgatgatac aggcaaaaat tcagttacac cctggatgat ccccaggatg cagaaaataa
     4321 caatactgta ggccatgtta aatctcctga atgatagttt caatgagtgg cgtattgttg
     4381 gtaaaccgat gggcaatcat tttggcaagc ttttttactg cgactgtcag gtgttcaggt
     4441 gaggcattct gtaaaagctg cttttcaata gtgttcgata gttccttttg aagcacctgc
     4501 ataaatctat cattctttat gccaataaaa cccctggcta taataactgg cgggtacagt
     4561 agcaacccat cacctataac aatgctgata acaatgacac catcagatgc caatgtttta
     4621 cgttcagcaa tgatgtcatc agtgatatcg ccaatatagg aaccatcaac atatacggta
     4681 gagagaggta tattcccctt cttttcaaaa cggtttggtg ttagctctat tatatcgcca
     4741 ttctgggcaa caatgatatt ctgtggtttt atgttgagtg attctgccag tgaggcatgt
     4801 gctttaagat gccggtattc gccatgtacc ggtaaaaagt attttggctg agtcagagta
     4861 atcataagct taagttcttc ctgtgacgca tgccctgaca catgaatttc ttcatcctgt
     4921 tcataaaata catcagcccc catctgcatc agggaattga taacactgta caccatgcgt
     4981 tcatttcctg gaataaccga agcggtaatg ataacggtat cccctgctcc tatcctgaaa
     5041 tgcttgtgcg tgccggtagc catacgggaa agagctgaca tgggttcacc ctgagaaccg
     5101 gtacacagaa ccaccagttt tttatgggta tacttgccaa tatcctttgc atctataatc
     5161 aggtcttttt tgtatttcag gtatcctagt tcagttgcta tctcaatatt tttttgcatg
     5221 gtggttccgg aaataacaac tttacgatta tatttctgtg aagcttccag aacctgctgg
     5281 attctgtgta tgttagaagc aaagcttgcg acaaaaatac gtccctttga ctttgcaaat
     5341 atttcaagga gcttcttttt cagcactatt tcagatggtg tataaccggg attggttgca
     5401 ttggtgctgt cactgatgag gagcagcacg ccctgctgac catattcagc aaagcggtga
     5461 aggtcagcaa cattaccatc aataggcgag tagtcaattt tatagtcgcc agtgtgtatg
     5521 atagtgccaa tgggagtttt tatggcaagc ccaacgccat caatgattga atgattcaca
     5581 cgtaaaaatt caatgacaaa tgaaccaata tgtattacat cacgtggctc tatctcaatg
     5641 aatgtaggtg catttaccgg ccgttcggca agtctgcttg ttaccagccc aagggttagt
     5701 tttgtggcat ataccggtac atttatcttt tgcagtaaaa agggtattgc cccaatatga
     5761 tcttcatggc catgggtaat gatgattcct ttaactttag ttttattctc aagtataaag
     5821 ctgaaatccg gtatcataaa atcaatacca ggtgtttcac cattggggaa ggtaatgccg
     5881 cagtctatta tgatcatttc attgtcatat tcaatggcca tcatgttacc gccaatagca
     5941 tgaaggccac ctattggtat aattcgtatt ttcttttctt tcataagcct gtgtgtccaa
     6001 agc
//
LOCUS       DAOSZP010000053         5969 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_11205_length_5969_cov_3.495096, whole genome shotgun sequence.
ACCESSION   DAOSZP010000053 DAOSZP010000000
VERSION     DAOSZP010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5969)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5969)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5969
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11205_length_5969_cov_3.495096"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="PK348_01985"
     CDS             complement(<1..215)
                     /locus_tag="PK348_01985"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008679395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051499 - D-aminoacyl-tRNA deacylase
                     activity [Evidence IEA];
                     GO_process: GO:0006399 - tRNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HXK65012.1"
                     /translation="MRAVIQRVTRATVEVEGNIIASINKGLCVLVGFTATDTEKDLQY
                     ICQKIIGLRIFDDENAFMNLSIADVNG"
     gene            complement(219..533)
                     /gene="trxA"
                     /locus_tag="PK348_01990"
     CDS             complement(219..533)
                     /gene="trxA"
                     /locus_tag="PK348_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015180335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HXK65013.1"
                     /translation="MGNIVEVNDINFNSEVLEHPGKVLVDFWAPWCGPCRMQTPILEK
                     LAQDPATKTKIVKVNTDNNPETAQRYGISSIPTLILFENGKEIDRMVGVQPETVLKNK
                     LS"
     gene            942..1664
                     /locus_tag="PK348_01995"
     CDS             942..1664
                     /locus_tag="PK348_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HXK65014.1"
                     /translation="MKSIINLFKFIWLFFWASFMSVVLFIPMTVAAIFSVTGNLAFNI
                     SKLWAWTMLIVTGVRVHIKGRETIKEGMQYVIISNHQSQYDILALVTSLKIQFRWVIK
                     KELLYVPLFGWALYAAKNVFIDRSNREKAIASINKAVNRLPQGVSLLVFAEGTRSKDG
                     TLQKFKKGGFTIAIERKMPILPVVVKGSRAILPKGSLIFHSGNIEVVVCDPIPADQYT
                     HETIEDLINKTHNVIEHELSVS"
     gene            complement(2077..2640)
                     /gene="thpR"
                     /locus_tag="PK348_02000"
     CDS             complement(2077..2640)
                     /gene="thpR"
                     /locus_tag="PK348_02000"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HXK65015.1"
                     /translation="MNRLFIALPIDEGIIQNLKPVYAMLSQYNDVLKVVEPSLYHLTL
                     KFIGECEGNVARAIESEFASLRFTIPPLPYVVKSIGMFPNSKNPSVIWTAIETNQKSI
                     NELVTCIQNFVKKYGIKEEDRPFVPHLTLARIRKGRKLTETIHKRLQTFATQEFGSAT
                     FTRVILFSSKLTPQGPVYTELQEINFA"
     gene            complement(2637..2933)
                     /gene="gatC"
                     /locus_tag="PK348_02005"
     CDS             complement(2637..2933)
                     /gene="gatC"
                     /locus_tag="PK348_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010277370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HXK65016.1"
                     /translation="MRFDESVINKVCELARLELTAEEKQEFTTQLEDIIRYVEKINEL
                     NTASIQPADHIVPLSNVFREDIAKESLPVEDVEHIAPQFEKSHFVVPRIIEEIE"
     gene            complement(2933..3616)
                     /locus_tag="PK348_02010"
     CDS             complement(2933..3616)
                     /locus_tag="PK348_02010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65017.1"
                     /translation="MHNHITNIKVLSIVCVLALLLACKTQFGLVHTVKSLDELPVAVK
                     NLGDISVTIEPFTEETWVRIAAGSQFIDRSKSGRLPKIPFFLILIENKSSQPIILKEI
                     AITYGKTRSPMLTNDECKSYLSSPAYGFINVDAIITPQRLLAYSGKVQNIDFSSDVIP
                     CTLPFIPSGDIHCMVVAGKWIPVQERQFTVEITIKSPDNEKIVDCTFVRKEYRTKGNH
                     FLVPDGRND"
     gene            3768..5966
                     /locus_tag="PK348_02015"
     CDS             3768..5966
                     /locus_tag="PK348_02015"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HXK65018.1"
                     /translation="MKRYIVIYSISIVLLCAACSSKPVVTKLLQTDLTVEYNLQLLNE
                     LYSDTVYVDPWLQKPWIVKVAHDIDKEKKWSKATRSLVIAATKQSAGKVLFPLQHGGK
                     LSFDCASMGQGRLIVQLLSPTKKKVLGEYSLSSLPFTHVQCNIPHSFADAKLVMEFQS
                     YSKDPAYCFVANAVVRHRDNDFKKPNVVFISVDALRADAVHCIIPKYNITPNMDALAA
                     DGAAFTRHFVVANWTRPSTIAMLSSVYGSATGVNIYYFQVSKQEKHYFYTQSGVVPLP
                     VLFGNNGYITRSIGNNAFILDHSGIGVDLGFDDMSEYERQWEDTMDIADEVIQWITAN
                     NDKPFFLFINFNAPHNAYIPPPQYLDPLRKSIKHVHPWFRRYLGEVAYTDDYIGRVVT
                     ALKKLNLYDNTIIVVTTDHGEVFNSVHEHSPYTDKKSIFTHGQTMYDEELHVPLIIKL
                     PKNIFSDSKAVPYQVRNIDIAPTVCELSDIPVPDSHQGKTLMSIIAGKELQDRPLYSE
                     GRLMYAVRIDNFKYVEKFYGFGIKPHHWGGAQVDEYFELYDLNQDPDELHNIVAVDTK
                     KAFEMQQKLYALRFKQPENILYAKKDVRGSISVREGFFYTVESSGVVKKISRQKYMFS
                     LKKGQRLVFSTIPSRTACYIQSDGSVALWGYSGTALPETDGMYVFDTTQPLLQKMPDD
                     AALHLFPDELIVWSKPQKGGIQKVSEHVAISDEMNTLLKQWGYIQNGGTR"
BASE COUNT         1802 a         1056 c         1203 g         1908 t
ORIGIN      
        1 tcaccattaa catcagctat tgacaggttc atgaaggcat tttcatcgtc aaaaatacgc
       61 agacctataa ttttttggca tatgtactgt aaatcttttt cagtgtcagt ggcagtaaaa
      121 ccaactaaaa cacacaatcc tttgtttata gatgcaatga tattcccctc aacttcaaca
      181 gttgcccgtg ttacccgctg tatgactgca cgcattggtt atgaaagttt atttttaagt
      241 acagtttcag gctgaacgcc taccatacgg tcaatttctt tgccattttc aaaaagtatg
      301 agagttggta tagatgaaat gccgtaccgc tgggctgtct cgggattatt gtcagtattg
      361 acttttacta tctttgtttt tgttgcggga tcctgagcta atttttctaa aattggtgtt
      421 tgcattcggc aaggcccaca ccatggagcc cagaaatcaa ccagcacttt gccaggatgt
      481 tcaagtacct cagaattgaa attgatgtca ttaacttcaa caatatttcc catacatacc
      541 tccacaaaaa ttcaagaaac aataatagca tggtaaaacg ttaattcaac aattatttta
      601 aagatagtac actaattatt ttttgaaaaa ttatataagt taatgcaata tccagtcttt
      661 ccgcccttag ctatggatgt tgtatacgtt actttcatgt atacatgact attggttctg
      721 gatgtttata tatatagatt aatacaccat tgcaattatt gtcaacacaa aagaatgaac
      781 aataaagata tcccatgatt tttttacttg cagttaacat tatgttgtgg ctatttacta
      841 aaacatttat actgagtcta tgatatcctt ttgaattgac aatgaaaatg tatattgtat
      901 aataatataa taaatgaaat tttgtttttt ggtatttgtt tatgaaatct attattaatc
      961 tttttaaatt catctggctt ttcttctggg catcatttat gagtgtggta ctttttatcc
     1021 ccatgactgt ggcagcaatt ttttcagtaa ctggaaatct ggcatttaat atttcaaaac
     1081 tgtgggcatg gaccatgctg atagttacag gcgtacgagt acacattaaa ggcagggaaa
     1141 caattaagga aggcatgcag tatgttatta tcagcaatca tcaatcacag tatgatattc
     1201 ttgcacttgt tacttctttg aaaattcaat tcaggtgggt tattaaaaaa gaattgctgt
     1261 atgttccgct ctttggatgg gcattatatg cagcaaaaaa tgtgtttatt gaccgtagca
     1321 acagggaaaa agccatagcc agcatcaata aagcggttaa ccgtttgcca cagggagtaa
     1381 gtcttcttgt ttttgctgaa ggaacacgtt caaaagatgg gacattacaa aaatttaaaa
     1441 aaggtggctt tactatagct attgaacgca aaatgccaat tttgccagtt gttgtaaaag
     1501 gaagcagggc aatacttccc aaaggcagcc ttattttcca ctctggtaat attgaggtgg
     1561 tggtgtgtga cccaatacca gcagatcaat atacccatga gactattgaa gatttaatta
     1621 ataaaactca caatgttatt gaacatgagt tatcagtatc ataacttaaa accaccatcc
     1681 agggttcaac aaattcatat gagggtgaaa tcaatttcca aataactaga ttattcaggc
     1741 attccaataa aatcaggaca tctttagaat gatacgtgcg attgcttcat cccgactttg
     1801 tcgtgatttg taatgacttt gttagaatgt cactgcaagt gaagtgtggt agtctcaaat
     1861 aaaaggtaag gagtatgtat aaaaagggta gatttttagt ccgctgacat gtcctcaaaa
     1921 tacgatgaca gatagaaaaa tacttccttg ctatgaggtt actgaattta taaaaggtat
     1981 gaatttaaac ttttaactga tagtatcctg gtatttcaat aattcgggat tcctcaggat
     2041 ggtattttat aatgaaatct attctcaagg atgcatttaa gcaaaattaa tttcctgtaa
     2101 ctctgtatac acagggccct gcggggtaag cttggatgaa aaaagtatta cacgggtaaa
     2161 agttgcacta ccaaactcct gtgtggcaaa tgtttgcagg cgtttgtgaa ttgtttcggt
     2221 gagcttacga ccctttcgta tgcgggcaag cgtcaggtgt ggcacaaaag ggcggtcttc
     2281 ttccttaatg ccgtattttt ttacaaaatt ctgaatgcag gtaacaagtt cattgatgga
     2341 tttctgattg gtttctattg ctgtccatat aaccgatgga tttttactgt ttggaaacat
     2401 tcctatactt tttactacat agggtagggg tggaatggtg aaacgtaatg atgcaaattc
     2461 agattcaatt gcccgtgcaa cattgccttc acattcccca ataaatttca atgttaaatg
     2521 atataacgaa ggttcaacaa cttttagtac atcattgtac tgtgaaagca tagcatatac
     2581 gggttttaaa ttctggataa ttccttcgtc aatgggtaat gctataaaaa gcctgttcat
     2641 tctatctcct caataatgcg aggtacaaca aaatggcttt tttcaaattg tggtgcaata
     2701 tgttcaacgt cttctactgg caatgattct ttagctatgt cttctctaaa cacattggat
     2761 aatggaacaa tgtgatcagc aggctgtata ctggctgtgt taagctcatt tatcttttca
     2821 acataacgga taatgtcttc aagctgtgtg gtgaattcct gcttttcttc agcggtcagc
     2881 tctaaacgtg ctaattcaca tactttgtta atgactgatt catcaaatct cattaatcat
     2941 tcctcccatc tggaactaaa aagtgattgc cttttgtcct atattcttta cgtacaaatg
     3001 tacaatcaac tattttttca ttatcaggag actttattgt aatttcaacg gtaaactgac
     3061 gttcttgcac aggtatccat ttacctgcta ctaccatgca gtgtatatcc cctgatggta
     3121 tgaaaggcaa tgtacatgga ataacgtcgc ttgaaaaatc aatgttttgc actttgcctg
     3181 aatacgcaag caatcgctgt ggagttatta tcgcatcaac atttataaaa ccatatgctg
     3241 gtgaagaaag atagctttta cattcatcat tggtcaacat tggggagcgg gttttgccat
     3301 atgttattgc aatctcttta agtatgattg gctggctgct tttgttttct ataagtatta
     3361 aaaaaaaggg tatttttggt agtcttccac tttttgacct gtcaatgaac tggctacctg
     3421 cggcaatccg tacccaggtt tcttcggtga atggttctat ggtaacagaa atatcaccaa
     3481 ggtttttcac agcaacaggc aattcatcca atgactttac cgtatggaca aggccaaact
     3541 gtgttttgca ggccagtaaa agagccagca cacatactat agagagtact tttatattgg
     3601 taatgtgatt atgcatggta tgcaaacaat aacagggtga tagttcattt gtaaagtttt
     3661 ttacacataa tcacgtaaga aaattatgag atagtaaatc aaaattatat tgcaataata
     3721 ctggatggta tatccatgtg tttgtaatta tacgttactg gggaatcatg aaacgatata
     3781 ttgttatata tagtattagc atcgtgttgc tttgtgctgc atgcagcagt aagccagtgg
     3841 taacaaaatt gcttcagact gatttaactg ttgagtataa tctacagctt ctgaacgaat
     3901 tgtattctga tactgtgtat gttgacccat ggctgcagaa gccgtggatt gtgaaagttg
     3961 cccatgatat cgataaagaa aaaaaatggt caaaggcaac acgttcactg gtgattgctg
     4021 caacaaaaca aagcgcagga aaggttttgt ttccactgca acatggcgga aagctttctt
     4081 ttgactgtgc atcaatgggt cagggcaggc ttattgtaca gttactatcg cccacaaaaa
     4141 aaaaagtatt gggtgaatat agcctgtctt ccttgccatt tacgcatgta cagtgcaaca
     4201 tacctcattc atttgctgac gcaaagcttg ttatggaatt tcaatcgtat tcaaaagatc
     4261 ccgcatattg ttttgttgcc aatgctgtag tcaggcatag agataatgac tttaaaaaac
     4321 caaatgtagt gtttatttcc gtggatgctt tgcgcgccga tgcagtacac tgtattatcc
     4381 caaagtacaa cattactcct aacatggatg cactggcagc tgatggtgct gcgttcacca
     4441 gacactttgt ggtggcaaac tggacacgcc cttcaactat tgccatgctt tcgtcggttt
     4501 atggttcagc aacaggtgtt aacatttact actttcaggt gtcaaagcag gaaaaacatt
     4561 atttctatac acaaagcgga gtggtgcctt tgccagttct ttttggtaac aatgggtata
     4621 ttacgcgttc tattggcaac aacgcattta ttctggatca ttcaggtatt ggcgttgacc
     4681 ttggctttga tgatatgtcc gagtatgagc gtcaatggga agataccatg gatattgctg
     4741 atgaggtgat acagtggatc actgccaata acgataaacc attctttctt tttattaatt
     4801 tcaatgctcc acacaatgcc tatatcccac ccccgcagta tttggatcca cttagaaaat
     4861 caattaaaca tgtgcatccg tggttcaggc gatatttagg tgaggttgct tacactgatg
     4921 attatattgg gagggtggtg acagcgttga agaagctaaa cctgtatgat aataccatta
     4981 ttgtggttac caccgatcac ggtgaagtgt ttaacagcgt acatgaacac agcccataca
     5041 ctgataaaaa gtcaatattt acccatgggc agacaatgta tgatgaagaa ttgcatgtgc
     5101 cattaattat caagctgcca aaaaatatct ttagtgattc taaggcggtg ccctatcagg
     5161 tgcgtaatat tgatattgcg ccaacggtgt gtgaattaag tgatatacca gtgcccgata
     5221 gtcaccaggg aaaaactctt atgagtatta ttgcaggcaa agaactgcag gacaggcctt
     5281 tatacagtga aggcaggctc atgtatgcag tcaggattga caatttcaag tatgttgaaa
     5341 aattttacgg atttgggatt aagccgcatc actggggtgg ggctcaggtt gatgagtatt
     5401 ttgaattata tgatttgaat caggatcctg atgagctaca taatattgtg gctgttgata
     5461 caaagaaagc gtttgaaatg cagcagaaat tgtatgcact acggtttaag cagccagaga
     5521 atattctgta tgctaaaaag gatgtcaggg gtagcatcag tgtccgtgaa ggattttttt
     5581 atacggtgga gtctagtggt gtggtgaaaa aaatttcacg gcaaaagtac atgttcagcc
     5641 ttaaaaaggg gcaacgcctt gttttttcaa ctattccatc acgcactgcc tgttatattc
     5701 aatctgatgg cagcgttgcg ctgtggggat acagtggcac tgctcttcct gaaactgatg
     5761 gcatgtatgt ctttgataca acacagccac tgttacagaa aatgccggat gatgcagcgt
     5821 tgcatctatt tcctgatgaa ctcattgtgt ggagtaaacc acagaagggt ggcatacaga
     5881 aggtatcaga acatgtagcc atttcagatg aaatgaatac gcttctcaaa cagtggggtt
     5941 atattcagaa cggtggtacg cgctaagtt
//
LOCUS       DAOSZP010000054         5866 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_11441_length_5866_cov_3.441920, whole genome shotgun sequence.
ACCESSION   DAOSZP010000054 DAOSZP010000000
VERSION     DAOSZP010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5866
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11441_length_5866_cov_3.441920"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1770
                     /locus_tag="PK348_02020"
     CDS             <1..1770
                     /locus_tag="PK348_02020"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002645703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HXK65019.1"
                     /translation="AVITISLALGARKMVNQNALIRKLPAVETLGSVTYICSDKTGTL
                     TQNKMTVETIFVNNTMFTATQPLQDNRSKSSQLFFYALALNNDAIRDTSGTLLGDPTE
                     TALYYYAFTNSFDKDLLTRTFPRVAEIPFDSERKCMTTIHKNTNQEITQSPYIVFTKG
                     AVDTIIDSANTIMIHSEIVPINKDEILKVAEQLSEQGLRVLAVTSKFLDTLPSSITPE
                     TVESNLTFLGLVGMIDPPRQEVQEAVSECKSAGIKPVMITGDHPLTAKAIAKRLGIID
                     ENRPYIITGKELQELSLEEFEQKVEYISVYARVAPEQKLKIVKALQDKGQFVAMTGDG
                     VNDAPALKRADIGVAMGITGTDVSKQAAHMILLDDNFATIVKAVREGRRIFDNIRKFI
                     KYTMTSNSGEIWTIFLAPFFGLPIPLLPIHILWINLVTDGLPGLALASEPEEKGIMQR
                     PPRHPQESIFAHGLGAHIIWVGLLMGAVSIITQALSITLWNAHWQTMVFTVLCLSQMG
                     HVLAIRSEKQSLFTLGLFSNKPLLGAFILTFVLQMATIYVPYLNGIFKTHPLTLEELS
                     ITLALSSVIFIAVEIEKFLKRKK"
     gene            complement(1824..2474)
                     /locus_tag="PK348_02025"
     CDS             complement(1824..2474)
                     /locus_tag="PK348_02025"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXK65020.1"
                     /translation="MAIVPELIKVFIIEDENLVRDTLAQSLELVDNINVVGTSENISS
                     SIGDIKATKPDVLIVDVRLKNENGIEASKNVLKLMPAVKIIILSGYFNDSLAANALAA
                     GASAFLSKSIAISHLVAAIFHVMVHNSFYAPQLPDEVLEDIMVKNKTSKRLCALSKRE
                     KEVLQLIAKEYSTKDIATMLGISEKTVRNHKSNIMAKLGITSHVGLIKYAYYMAMI"
     gene            2839..4218
                     /locus_tag="PK348_02030"
     CDS             2839..4218
                     /locus_tag="PK348_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013825103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXK65021.1"
                     /translation="MDKVSPATRKTALAVTTLGSFLTSFMGSSINVALPIIGKEFAID
                     AIVLSWIATSYLLAAAMFLVPFGRIADIYGRKKIFTYGVILYTFSSFLTAIAQSALIL
                     ILFRVLQGISGAMIFSTGAAMLISVFPPQQRGRVLGINVSAVYIGLSTGPFLGGILTQ
                     HLGWRSIFAISVIVGVAIVLLIYSKLEGEWVEADGETFDFTGSLIYAIALTMVMFGFS
                     SLPSVFGTIMIIGGIAFIFLFIKWELHTRHPVLDMNLFKNNTVFTFSNLAALINYSAT
                     FAVTFIVSLYLQYIKGISPQSAGIILISQPVTMALFSPIAGRMSDKIQPQVIASWGMA
                     FTTIGLLLLTFLHLHTSILFIITCLIFIGFGIALFSSPNTNAVMSSVHKKYYGVASGI
                     LGTMRLTGQAFSMGIVTLILAVHMGRVQITPLYFDRFLQSSQTAFIIFTLLCFGGIFA
                     SLARGKIHR"
     gene            4390..5514
                     /locus_tag="PK348_02035"
     CDS             4390..5514
                     /locus_tag="PK348_02035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00341 family protein"
                     /protein_id="HXK65022.1"
                     /translation="MKKQNNNETNPEKVQDSLVQKFKNMVDTVIQPAEHSMRNYVNIN
                     FWRTKYNEYKKIREKEKEDLDIYETLSYGARPTIEYYLLTLLSCLIATAGLLQNSPAV
                     IIGAMIVAPLMTPILAFSLGVIWGDTQLIKTSFSSIIKGSVLAILISGTLAFFVPIAE
                     YTSEILARTKPNLFDIVIAISSGLVGAYGNANKKISNTLVGIAIAVALMPPLCTIGIG
                     VGNFDWHIARGALILFSINLISISLAGAIIFWIMKIHPKSREEKENVTRRALSQIIIS
                     VILLTIIAIPVGIYTYRTFLLEHAKKKVWVTAKKYFGTDVFAIQFDENQGKLDVTVIV
                     LSQQADEESLKQVQNIKSLHPDFGTVNIKFIQTVSSNTTD"
     gene            complement(5619..>5866)
                     /locus_tag="PK348_02040"
     CDS             complement(5619..>5866)
                     /locus_tag="PK348_02040"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002718201.1"
                     /note="Catalyzes the synthesis of acetoacetyl coenzyme A
                     from two molecules of acetyl coenzyme A. It can also act
                     as a thiolase, catalyzing the reverse reaction and
                     generating two-carbon units from the four-carbon product
                     of fatty acid oxidation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HXK65023.1"
                     /translation="DLIEANEAFAAQAVVVNKEMGWDVTKVNVNGGAIAIGHPIGASG
                     ARILTTLIYEMIKRNAKKGIATLCIGGGQGVAVTVER"
BASE COUNT         1805 a         1160 c         1032 g         1869 t
ORIGIN      
        1 gcagtaatca ccatttcact ggctctgggt gctcgaaaga tggtgaatca aaacgccctt
       61 atacgcaaac ttcctgctgt agaaacattg ggttcggtca cctatatttg ttcagacaag
      121 acgggtacgc ttacacaaaa taagatgact gttgaaacaa tttttgtcaa taacacaatg
      181 tttactgcaa ctcaaccact acaagacaat cgtagcaaaa gttcacagtt atttttctat
      241 gctctggctc tgaataatga tgccatacgt gatacatctg gcacactgtt aggcgatcca
      301 accgagactg cattgtatta ctatgccttt accaattcat ttgataaaga tttgctcact
      361 cgaacatttc cccgtgtagc tgaaataccg tttgattctg aaagaaagtg catgaccacc
      421 attcataaaa atacaaacca ggaaataacc caatcaccgt acattgtatt taccaaagga
      481 gctgttgata ccatcataga tagtgccaac actatcatga ttcattcaga gatagtaccc
      541 atcaacaaag atgaaatttt aaaagtggct gaacaactct ctgagcaggg actacgagtg
      601 ctggcagtta catcaaaatt tcttgatact cttccttctt caataacccc tgaaacagtt
      661 gaatcaaatc ttacattctt aggtcttgtt ggcatgattg accctccccg ccaggaagta
      721 caagaagctg tgtctgaatg taaatcagca ggcatcaaac cagtcatgat aacaggcgac
      781 catccactga cagctaaagc catagccaag cgccttggca ttattgatga aaaccgaccc
      841 tatattataa caggcaaaga attgcaggag ctatcgcttg aagaatttga acaaaaggta
      901 gaatacatta gcgtttatgc aagggttgcc cctgaacaaa aactaaagat tgtaaaagca
      961 ttgcaggata aaggccaatt tgttgccatg acaggcgatg gtgtcaatga tgcacctgct
     1021 ttgaagcgag cagatattgg tgttgccatg ggaataacag gaaccgatgt atcaaagcag
     1081 gcagcacaca tgattttact agatgataat tttgcaacca ttgtaaaagc agtcagagaa
     1141 ggaagaagga tctttgacaa tattagaaaa tttataaaat acactatgac aagcaattca
     1201 ggtgaaatat ggacaatatt tttagctcca ttttttggct tacccattcc actgcttcct
     1261 atacacattc tatggataaa tcttgtcact gatggtttgc ctgggctggc attagcttct
     1321 gagcccgaag aaaagggaat catgcaacgc ccacctcggc atccgcagga aagcattttt
     1381 gctcatggcc tgggtgctca cattatatgg gtgggattgc tcatgggagc tgtttctatt
     1441 attacgcaag cactttcaat aacattatgg aatgcccact ggcaaacaat ggtattcaca
     1501 gtgctgtgtt taagccagat gggacatgta cttgccatac gttcggaaaa acagtctttg
     1561 ttcacgcttg gccttttctc aaacaaaccg ctgctcggag cgtttatact cacatttgtg
     1621 ctacaaatgg caacaatata cgttccctac ctcaatggga tttttaaaac gcatccatta
     1681 acacttgagg aattgagcat aacacttgca ttatcatctg tgatatttat tgccgttgaa
     1741 attgaaaagt ttcttaaacg taaaaaatag tacattgggc gatagtaact gataaaaaac
     1801 ctaacgtttt ttataagcta ttattaaatc attgccatgt agtatgcata cttgataagg
     1861 ccaacgtggc ttgtaatgcc caattttgcc atgatgttgg atttatggtt acggacagtt
     1921 ttttcgctga ttccaagcat ggtagcaatg tcttttgttg aatactcttt tgcaataagc
     1981 tgcaaaactt ctttttcccg ctttgaaagt gcacatagtc tttttgatgt tttgttcttc
     2041 accattatat cttcaaggac ttcatcaggt aactgtggtg cataaaaact attatgaacc
     2101 ataacatgaa atatagcagc caccagatgt gatattgcaa tggattttga taaaaaagcc
     2161 gaagctcctg ctgccagtgc atttgcagca agcgagtcat taaaataacc cgaaagtatg
     2221 attattttta cagcaggcat taattttaaa acatttttac tggcttcaat cccattttca
     2281 tttttaaggc gcacatcaac aataagcaca tctggttttg ttgctttgat atctccaata
     2341 gaagaggata tgttttcaga agtgccaaca acattaatat tatcaactag ctccaggctc
     2401 tgagccaggg tatcccgtac aaggttttca tcttctataa taaaaacctt tataagctct
     2461 ggtacaatag ccatatattt aatccatttt ttctatatat tgtgctttat tatacatcat
     2521 cgcctgtttt agtcaatagg caaatgtcct aataaatggg cactttcact aaaataatta
     2581 atttttattc tctatcaata catatttttt atataatcct ttcctcctgt tactattact
     2641 tgctttatta ttaattcaac tacacaatac attccccctt actgataaat agtgttaata
     2701 ggtttttcga ctatattttt tatatagaaa gattatgata agctggtaga atagtaccta
     2761 aatatatttt tatatgtctt gatactatac tttaaaatta atggcataaa tttctcattg
     2821 tacataagga tatctaccat ggataaagtc agtcctgcaa ccagaaaaac agcattagcg
     2881 gttaccacct taggctcatt tctcacatca tttatgggtt catcaattaa tgttgctctt
     2941 cctattatag ggaaggaatt tgccattgat gcaattgtgt taagctggat tgcaacatcg
     3001 tatctattag ctgcagctat gtttctggta ccttttgggc gaatagccga tatctacggc
     3061 aggaagaaaa tatttaccta cggcgtcatt ctctatactt tttcatcatt cctcacagct
     3121 attgcacaat ctgcccttat actcattcta tttagagtgc tccagggaat aagcggtgca
     3181 atgatattca gcacaggggc tgccatgctc atatcagtat tccctccaca gcagcggggc
     3241 cgtgttctgg gtattaatgt ttcagcggtt tacatagggc tttcaacagg ccctttttta
     3301 gggggcattt taacacaaca tttaggatgg cgcagtatat ttgcaataag tgttatagta
     3361 ggggtagcga tagttctatt aatatattcc aaactggaag gagaatgggt tgaagctgac
     3421 ggtgaaacgt ttgattttac tggatcgctc atctatgcaa ttgcgttaac catggtgatg
     3481 tttggatttt catctcttcc gtcagtattt ggaacaatta tgataatagg tggaattgcc
     3541 ttcattttcc tttttataaa atgggaacta cacaccagac atcctgttct tgacatgaat
     3601 ctttttaaga ataatactgt ctttacattt tcaaatctgg cagcgttaat taattacagt
     3661 gcaacatttg cagtaacatt cattgtaagc ctttatctgc aatatataaa aggaataagc
     3721 ccgcaaagtg ctggcatcat ccttatttca cagcctgtta ccatggctct gttttcacct
     3781 atagcaggga gaatgtccga caaaattcag cctcaggtta ttgcttcctg gggtatggct
     3841 tttaccacaa ttgggcttct gctcttaaca tttttacatt tacatacatc aattttattc
     3901 atcatcacct gcttaatttt cataggtttt ggcatagcac ttttttcttc acccaatacc
     3961 aatgctgtca tgagctcggt acataaaaaa tattacggtg ttgcttctgg tatactgggg
     4021 acaatgcgcc taaccgggca ggctttcagt atgggcattg ttacattgat tctggcagta
     4081 catatgggaa gagtacagat aaccccactg tattttgaca ggttcttgca aagttcacaa
     4141 acagcattta taatattcac attgctgtgc tttggcggga tatttgcatc acttgcacgt
     4201 ggtaaaatcc atagataagt attgttatct gaagcattat atatttttgc accaaaacct
     4261 gcaataacat ctgaaatgat aaaaatgcac agcaacctta cctatttatt acttgattta
     4321 atcaattgtt ttcagtatta tgtgttacat actatttggt aaatctcatt ccagcttcag
     4381 gagtgcatta tgaaaaagca aaacaataat gaaacaaatc ctgaaaaagt tcaagattca
     4441 cttgtccaga aatttaaaaa tatggttgat acagttattc aacctgcaga acattccatg
     4501 cgtaattatg ttaacattaa tttctggcgg acaaaataca atgaatataa aaaaattcgt
     4561 gaaaaagaaa aagaggacct tgatatatat gaaacacttt catacggtgc acgcccaact
     4621 attgaatact atttattgac attgttatcc tgcctcattg caaccgcagg ccttctgcag
     4681 aattcgccag cagtaattat tggtgccatg atcgttgctc cacttatgac accaatatta
     4741 gcgttttcat taggggtcat ctggggtgat acacagctta ttaaaacatc gtttagttca
     4801 attattaaag gttcagttct ggcaattttg atatcaggca ctttggcatt ttttgtcccc
     4861 attgctgaat ataccagcga aatacttgca cgaacaaaac caaatctttt tgatattgtt
     4921 attgccattt catcaggcct tgttggtgca tatggcaatg caaataagaa aatcagcaat
     4981 acgcttgtag gtatagctat tgctgtggcc ttaatgccac cattatgcac cattggcatt
     5041 ggagttggca actttgactg gcatattgca cgtggagcac ttattctttt ttcaatcaat
     5101 ttgatatcca tttcactggc aggtgcaatt attttctgga ttatgaaaat acatccaaaa
     5161 tccagagagg aaaaggaaaa cgttacccgg agagctcttt cacagattat tatttcagtt
     5221 atactgttaa ccattatcgc aataccggta ggaatttata cttacagaac ctttctactg
     5281 gaacatgcaa aaaagaaggt atgggtaaca gcaaaaaaat attttggtac cgatgttttt
     5341 gctattcaat ttgatgaaaa tcagggaaaa cttgatgtaa cagttatagt gctgtcacaa
     5401 caggctgatg aagaatcgct caaacaggtg caaaatataa aatcattaca tcccgatttt
     5461 ggtacagtta atattaaatt cattcaaact gtatccagta atactactga ttaacttttc
     5521 aagcaaatca aattatattc attttataca cagttttgat ttcaactaaa aagggagcac
     5581 ccaggctccc cttttaaaat tataattttc aacagttact atcgctccac tgttactgca
     5641 acaccctgtc caccaccaat acacagtgtg gctatgcctt ttttagcatt acgcttaatc
     5701 atttcataaa ttagtgttgt taaaatacga gcaccacttg ccccaatggg atgccctatc
     5761 gcaattgctc caccattaac attaaccttt gtaacatccc agcccatttc tttgttaacc
     5821 accacagcct gggcagcaaa tgcttcattt gcttctatta aatcta
//
LOCUS       DAOSZP010000055         5832 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_11516_length_5832_cov_3.451792, whole genome shotgun sequence.
ACCESSION   DAOSZP010000055 DAOSZP010000000
VERSION     DAOSZP010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5832
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11516_length_5832_cov_3.451792"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(81..1655)
                     /gene="murJ"
                     /locus_tag="PK348_02045"
     CDS             complement(81..1655)
                     /gene="murJ"
                     /locus_tag="PK348_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HXK65024.1"
                     /translation="MKTESKDKSLFNTVMVATGIFLSRIAGLIRDRVFAHYFGNSDAA
                     DAFRAAFRIPNFLQNLFGEGVLSASFIPVYAKLLAEKKFDQATKLARSIAGILSLVIS
                     SLVFVGILITPLLIDIIAPGFQGEKRELTILLVRIFFPGAGILVFSAWCLGILNSHGK
                     FFLSYSAPVFWNLIIIITLLFFGDSSTQNDLATYTAAGSVVGSLMQVLIQLPFVLTIV
                     KNVTLRFDTQSREVRTVIKNFIPVFFSRGVVQVSAYVDSLLASLLPTGAVAALSYAQT
                     LYMLPISLFGMSVSAAELPSMSKVIGPQEEIASKLRNRINNSIKRIAFYIVPSTVAFF
                     ALGDILVGAIYQTGKFTTHDTFFVWMVLAGSTIGLLAITQSRLYSSAYYAMNDTRTPL
                     KFAIIRVLLTSVTGYYASQYGPLLFHIDMVYGTVGLTITAGLSGWVEYKMLQVSISRK
                     IGTVGLEKKYLFYLWTSALIAATIAFFIKINLHYHPLILAVTLLPLYGVLYFFFAFIF
                     RIEEIMNILKMVGFKK"
     gene            1943..3910
                     /locus_tag="PK348_02050"
     CDS             1943..3910
                     /locus_tag="PK348_02050"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HXK65025.1"
                     /translation="MKKIIIYTIIMVTIASMSVAGDWPIYKGNLFFTGNNDEIIVSNN
                     QLKWLFLAKDRVFNPVVSDGRIYFVDKNAFLYCIDEDSGNIIWLNDVRSISSQFKAYS
                     KSAGKIKYPLVYGNTVIVSDPIAIYAFDKRTGNVIWARTGLRVDETKPQGLTGITTQI
                     SIDGIYADPIIDQDTIYYGTRKIFASRDIKNGHIIWENKDIATYSGFPVYYDQYIITQ
                     SMNYETKTFTVFCLEQRTGKVVWQRNFNQPVTILPPVVNKHVYVPINNAVYCLDIKNG
                     ETVWQKSYNGTITSPLSFTNRSILFSLDNASIVGINPENGSTIANIFVGQQSSPYFVT
                     IRDVIYIVYNQHNDKNQPVAKVKAMYINSGNIWWEFTAPFPGGVSQPVASDGILFLPA
                     GNYLYAIGAGYMGSQSYESSKPEETKKGEPQEPSKLPDQKLSEKELADKTITPESKPA
                     PQPAKQEMPTRKLPVEITDNNKAPLKSRITIQYKDENGEYNQSTAVNGKGDITIPDKD
                     NVEVIFEADGHVPKKVIVNKSDSQLKIVLDEIQPGKAVVVDNIHFDFDKWYIRKDSIN
                     ILDKLVDMMKANPYLKVEVWGHTDSIGDDTYNQKLSEKRADAVCDYIIKHGISPERIS
                     AKGFGETKPIASNDTKEGRQKNRRTEFYFKS"
     gene            complement(3895..>5832)
                     /gene="recJ"
                     /locus_tag="PK348_02055"
     CDS             complement(3895..>5832)
                     /gene="recJ"
                     /locus_tag="PK348_02055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HXK65026.1"
                     /translation="HRVADLLLLRGIDSPYSAYTFLNPDLTLCYSPFLMNEMDEAVRI
                     IKNAVSNKKKIGIFADSDIDGLTSLTLLTHLLDLLNVKYFYRYPVGDELYGLTISIIE
                     EFKKNNIDCIITLDSGTRDVNEIAYARSLGMDVIVCDHHEPSETLPDAAIINPKLMTS
                     QYPFKELAGVGVTYKLCLAVLLSYTKYYDKYIIVIALSHDSILYKVINRGRAIAHGSA
                     SSIQEIKHLSKQYTGAILFNYDVPDAALLQHGMHYDIRDFVDELSPIHQIIKGDIKDL
                     FPSPVDYATMLLFKIQYQQSKKISQFIQDMLPLVALGTIADMMPLIDENRIIVSNGIS
                     YFDKTNHCGLKVIREYIAKTINSDTIGWDISPLLNTPGRFGKTKLAADFLLEKKPSKV
                     KELLQEIIQLNKERKTIIQTLVSKYSNDDSNENHALRVICSDEIPDGLTGIIANRLAD
                     QLLQPVLVISDKPDAEIIKGSGRSRNNFDFFTSVSKYEHLFEKLGGHNHAFGFSIKKE
                     NIPILLKELDHTLCYEATENNIPEYDYELLVEDITFELLEELKLFEPYGIGHKPFIFL
                     SKNCTISQYFIINGKHCKLIINGSIKLEAMAWNSADKYNDILTQKIPVDILFTVKANE
                     FNGLISPLLIIENLYPSQDLK"
BASE COUNT         2037 a          989 c          992 g         1814 t
ORIGIN      
        1 cgaatgatag aatggattaa aaatattatg gtgtaaaaat ttttaagcct ggttttaaac
       61 cagggtaccc atactattta ttatttttta aagccaacca ttttaagtat attcattatt
      121 tcttcaatac ggaatataaa agcaaagaaa aagtacaata caccatataa cggaagcagt
      181 gtcactgcaa gtattaaagg atgataatga aggttgatct taataaagaa tgctattgta
      241 gctgcaatga gggcacttgt ccataaataa aataaatatt ttttttccaa accaacagta
      301 ccaatctttc ttgatatact tacctgtaac atcttatatt caacccatcc tgacaaacct
      361 gcagtaatcg ttaaaccaac agttccatag accatatcaa tgtggaaaag tagaggtcca
      421 tactgtgaag catagtaacc tgtgacactt gtcagtaata cccgtattat tgcaaacttc
      481 aatggtgtac gggtgtcatt catggcataa tatgctgaag aatataaacg actctgtgtt
      541 attgctaaaa gcccaattgt tgaacctgcc agcaccatcc acacaaagaa tgtatcgtgt
      601 gtggtaaatt ttccagtctg gtagatagct cctaccaaaa tatcacctaa ggcaaaaaat
      661 gcaactgttg acggaactat ataaaatgca atgcgcttaa ttgaattgtt aattctattc
      721 ctcagctttg atgcaatctc ttcttgtgga ccaataactt tagacatgga tggcaattca
      781 gcagcggata ctgacatgcc aaatagactt attggcaaca tgtataatgt ttgtgcatag
      841 gataatgctg cgacagctcc ggttggcaac aggctggcaa gtaaggaatc aacgtatgca
      901 ctaacctgta ctacaccacg gctgaaaaaa accggtataa aatttttaat tacagttcgt
      961 acttctcttg attgagtatc aaaacgtaat gtaacatttt ttactattgt taacacaaaa
     1021 ggcaattgaa ttaatacctg catcaaactg ccaacaactg atccggctgc ggtataagtg
     1081 gctaagtcat tttgcgtgga actatcgcca aaaaataata atgttattat gattataaga
     1141 ttccaaaata ccggtgcact atacgataaa aagaacttgc catgactgtt taaaatgcct
     1201 aaacaccagg ctgaaaatac aagtattcct gcgccaggaa aaaatatacg aaccagtaaa
     1261 atggtaagct ctcgcttttc accctgaaaa cctggtgcaa taatatcgat aaggagtggt
     1321 gttatcagga ttccaacaaa cacaagtgat gaaattacaa gtgacaatat tccagctatg
     1381 ctacgggcaa gttttgtggc ctgatcaaac tttttttctg caagtaattt agcatacacc
     1441 ggtataaatg aggctgaaag gacgccttca ccaaaaagat tttgcaaaaa atttggaatt
     1501 ctaaatgctg ccctaaacgc atcagcagca tcagaattac caaagtaatg ggcaaacacg
     1561 cgatctcgta taagaccggc tattctactt aagaatatac cggtggcaac cattactgtg
     1621 ttgaataaac ttttgtcttt tgattctgtt ttcatattaa aaatgacata tacgctctgc
     1681 tattgttggc atagaaatat ataaattctt taaatactat tttagtataa agctaatgca
     1741 ttatctatcc ctgtctttta cggcaggggc atataagcat ttcttacttt ataaagctaa
     1801 ggaaaaaata ttgattttta aaacaacaat tcacctgtca aaatttgtca atttgtaaaa
     1861 ataaaaaaat aaattatttc ttgtagttat tatacacata gcagataatt tgatgttagc
     1921 atgttttaag gaggcaatta ttatgaagaa aattataata tacactatta ttatggtaac
     1981 tatcgcttcc atgtcagttg cgggtgactg gccaatctat aagggtaatt tattttttac
     2041 gggaaataac gatgaaatta ttgtttcaaa taaccagtta aaatggctat tcttagccaa
     2101 agatagagta tttaatcctg ttgtaagtga tggaagaatt tattttgtag ataaaaatgc
     2161 atttctttat tgtatcgatg aagattcagg aaatattatc tggcttaacg atgttcgatc
     2221 catatcttca cagtttaaag catattcaaa atctgcaggg aaaattaaat atccacttgt
     2281 atatggtaat acagttattg tcagcgatcc cattgctatt tatgcttttg ataaacgaac
     2341 cggcaatgtt atatgggcac gtactggatt acgtgttgat gaaacaaaac ctcaaggatt
     2401 aaccggaatt acaacacaaa tttctataga tggcatatat gctgatccca ttattgatca
     2461 ggatactatc tattatggga cacggaaaat atttgcatca cgagacatta aaaacggcca
     2521 tataatatgg gaaaacaagg atattgctac ctatagtggg tttcctgttt actatgatca
     2581 gtatatcata acgcaatcaa tgaattatga aacaaaaaca tttaccgttt tttgcttaga
     2641 acaaagaacc ggtaaagtag tatggcaaag aaattttaat caaccagtta cgatattgcc
     2701 acctgtagta aataagcatg tatatgtacc aataaataat gctgtatatt gtttagatat
     2761 aaaaaatggt gaaacagtat ggcagaagtc atataatggc accatcacct cgccattaag
     2821 ttttacaaac aggtctatac tgttttcgct ggacaatgca tcaattgttg ggataaatcc
     2881 tgaaaacggc agcacaattg caaatatatt tgttggacaa caatcttctc cttactttgt
     2941 cacaatccgt gatgtaatat acattgtgta taatcaacat aatgataaaa atcaaccagt
     3001 agcaaaagtt aaagcaatgt atattaattc aggtaatata tggtgggaat tcacagctcc
     3061 cttccctgga ggagtatcgc agccagtagc atctgatgga attttatttt tacctgctgg
     3121 aaattatctt tatgcaatcg gtgcagggta tatgggtagc caaagttatg aaagttcaaa
     3181 accagaagaa accaaaaagg gtgaaccaca ggaaccatca aaattacctg atcagaaact
     3241 atctgaaaaa gaattggccg ataaaacaat aacaccagaa tctaagcctg caccacaacc
     3301 tgcaaaacaa gaaatgccaa cacgaaagtt gcccgttgag attacagata ataataaagc
     3361 accactgaaa tcacgaataa ccattcaata caaagatgaa aatggagaat acaaccaatc
     3421 aactgctgtt aacggtaaag gtgatataac aattcctgac aaagataacg ttgaagtgat
     3481 ttttgaagca gatggtcatg ttccaaaaaa ggtaattgtc aataaaagcg atagtcaatt
     3541 gaagatagtc cttgatgaaa tacaacctgg caaagctgta gtggtggata atattcactt
     3601 tgattttgat aaatggtaca tcagaaaaga ctctattaat atactggata aactggtaga
     3661 catgatgaaa gctaatccat acttaaaagt tgaggtatgg ggacatactg actcaattgg
     3721 cgatgataca tataatcaaa aattatctga aaaacgtgct gatgctgtat gtgattatat
     3781 tattaagcat gggataagcc ctgaaagaat cagtgcaaaa ggttttgggg aaacaaagcc
     3841 aatagcatca aacgatacaa aagaaggaag acaaaagaac aggagaactg aattctactt
     3901 taaatcctga gagggataaa gattttctat aatcagtagc ggcgatatta gcccattaaa
     3961 ttcattggct tttactgtaa aaagtatatc aacaggaatt ttttgtgtta agatatcgtt
     4021 atatttatct gcagaattcc atgccatagc ttctaatttt atagaaccat taataataag
     4081 cttacaatgt ttgccattta taataaaata ttggctgatg gtgcagtttt ttgataaaaa
     4141 aataaaaggc ttatgaccta ttccgtaagg ttcaaataat tttagttctt caagtaattc
     4201 aaaagtaatg tcttctacta ataattcata atcatattct gggatattat tctctgtagc
     4261 ttcataacac aatgtatgat ctaattcttt taacagtatg ggaatattct ctttttttat
     4321 tgaaaaacca aaggcatggt tatgccctcc taatttttca aaaagatgtt catattttga
     4381 tacacttgta aaaaaatcaa aattatttct tgaccgccct gatcctttaa ttatttctgc
     4441 atcaggttta tctgaaatta caagcacggg ttgtaacaac tgatcagcaa gtctatttgc
     4501 aataatcccc gttaaaccgt caggtatctc atcagaacat attaccctta atgcatgatt
     4561 ttcattagaa tcatcattag aatattttga aactaacgtt tgaatgatag ttttacgctc
     4621 cttgttgagt tgaataatct cctgtaaaag ttctttgacc tttgatggtt ttttctctaa
     4681 aagaaaatcg gcagccagct ttgttttgcc aaatctgcca ggtgtattga gaagtggtga
     4741 tatatcccag ccaatcgtat cggaatttat agtttttgca atatattctc ttattacctt
     4801 taatccacaa tgattggttt tatcaaagta tgatatccca ttactcacaa taattctgtt
     4861 ttcatctatc agaggcatca tatcggcaat agtaccaagt gccaccagtg gcagcatatc
     4921 ctggataaat tgtgatattt tttttgattg ctgatattgt atcttgaaaa gaagcattgt
     4981 ggcataatca acagggcttg ggaataaatc cttaatatca ccttttatta tttgatgaat
     5041 tggcgatagc tcatctacaa agtctctgat gtcataatgc atcccatgtt gtaataatgc
     5101 tgcatctggt acatcgtaat taaaaagaat tgcaccagta tattgttttg agaggtgttt
     5161 aatttcctgt atgctgctag ctgaaccatg agctatcgcc ctgcccctgt taataacttt
     5221 atataaaata gaatcatgtg ataatgcaat aactataatg tatttatcat aatactttgt
     5281 gtagcttaac aataccgcca gacacaattt ataggtaaca ccaacacctg ccagttcctt
     5341 gaaaggatat tgcgaagtca ttaattttgg gttaattatt gccgcatctg gaagagtttc
     5401 agatggttca tgatgatcgc atacaatgac atccataccc aaacttctgg catacgcaat
     5461 ttcattaaca tcacgagtgc cactatcaag ggtaataata caatcgatgt tatttttttt
     5521 aaactcttca ataatagata ttgttagccc atataattca tcgccaacag gatagcggta
     5581 aaagtacttt acattcaaaa gatcaagaag gtgagttaat aatgttaatg aagtgagacc
     5641 atcaatatct gaatcggcaa aaatgcctat cttcttttta ttacttacag catttttaat
     5701 aatccttact gcttcatcca tctcattcat aagaaatgga ctgtagcaca atgtaaggtc
     5761 aggattgagg aatgtgtagg cactataagg gctgtctata ccccttaata atagtaaatc
     5821 tgcaacacga tg
//
LOCUS       DAOSZP010000056         5824 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_11540_length_5824_cov_2.815566, whole genome shotgun sequence.
ACCESSION   DAOSZP010000056 DAOSZP010000000
VERSION     DAOSZP010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5824
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11540_length_5824_cov_2.815566"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1013
                     /gene="aroA"
                     /locus_tag="PK348_02060"
     CDS             <1..1013
                     /gene="aroA"
                     /locus_tag="PK348_02060"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HXK65027.1"
                     /translation="GNSGTSLYFGIGVASLIHGMTVFTGDHQIRNRPAEDLLNAITEL
                     GGYAVSTRSNGKPPVVVQGPIIGGHVSIRAITSQYLSSLLVATPKAKNNTIIDVPLLN
                     EAPYVGMTLWWLKELGIEYHNTGYEQFIVKGGQRYKPFTKYIPADFSSATFFMVAAAI
                     TQSSVVLKGLDFNDTQGDKEVVNILKAMGADVDIMPDAIAISGKPLKGGVFDLNAIPD
                     ALPALSVCACFADGETRLVNVPQARVKETDRIAVMCAELKKMGADIEELPDGLIIRKS
                     TLKGAHVNGHHDHRVVMSLAVAGLAAEGSTTIDTAESVSVTFPNFVELMQALGASIRK
                     EE"
     gene            1010..1414
                     /gene="aroH"
                     /locus_tag="PK348_02065"
     CDS             1010..1414
                     /gene="aroH"
                     /locus_tag="PK348_02065"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006716183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HXK65028.1"
                     /translation="MMVVRGVRGAITVDENSKEEIVHRTEELLRAIVTINKIQTNDIA
                     SAIFTVTDDLNAEFPAVAARKLGWIHIPLLCSREIPVPGSLGMCVRVLLHINTDKNPE
                     EIVHVYLRDARKLRPDIGTPEQDKYYVSDSQT"
     gene            1565..2344
                     /locus_tag="PK348_02070"
     CDS             1565..2344
                     /locus_tag="PK348_02070"
                     /inference="COORDINATES: protein motif:HMM:NF028441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HXK65029.1"
                     /translation="MKNIHSIMLVILLGVSIIFFSHDHSVAQADREIELTAQEILARV
                     DRIMDYPDGILKGYMMHITPDGSSQKLQVTGYINKNQFLFDIVKPARGSMQKILYRIG
                     GEDLWVYDMAARALFHKMGIDKYDDILATNFFYVDLSNADLQSNYVARIDGSVILKNQ
                     DCYKLTLDPIDKTGAYGKLTLYVIKQNYIPLRIDYHDQDKVIFKTLSIAKIGQKDGKS
                     FPIRYDMLNIKKGTMTVLEFTSIEDGATINTEIFRPEKLGD"
     gene            2346..3068
                     /locus_tag="PK348_02075"
     CDS             2346..3068
                     /locus_tag="PK348_02075"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HXK65030.1"
                     /translation="MAQFFVSAPGHDNTVVIEGKDYYHLVRVRRVRVGDSIDVIDTEG
                     VRYRATIAHIEAQKIIATIMQTITYPEISLNLTLCVALLKGKKFDLVIQKAVEIGVRS
                     IIPVVTERTIPDISEKEEKKLERWQKIATEAAKQCLRKDVPRVSSVMEYTDVLQLSKG
                     IGILAHADPEAQNLRQYLKNNPQHNDVMLLTGPEGGFSKKEVEIARQFGWIVLYNGAT
                     QLRAETAAIVLPAIIIYEWGYT"
     gene            3082..3279
                     /gene="thiS"
                     /locus_tag="PK348_02080"
     CDS             3082..3279
                     /gene="thiS"
                     /locus_tag="PK348_02080"
                     /inference="COORDINATES: protein motif:HMM:TIGR01683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HXK65031.1"
                     /translation="MRIRVNGIDIEFEKTTLKELLDLYRFNPSKIMVEKNGRIVEKEN
                     YATETLQDGDVLEIARFVGGG"
     gene            3276..3788
                     /locus_tag="PK348_02085"
     CDS             3276..3788
                     /locus_tag="PK348_02085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65032.1"
                     /translation="MIEKLRTALMESSLSHEELQHIMFLLFCCGDSNRQVPEDLIKAG
                     YISINDNCCTLTDKGRAYRDSLLQKLAKFHAASDEVFRQYFDNELLPIKKKMQLLKHE
                     WRSTRDKRLRYKETLMKQGKDKAAIKKDVQYKKLQRQQNTLRTRMRHAEKEYNKKLSA
                     CIKRIGILDL"
     gene            3800..5080
                     /locus_tag="PK348_02090"
     CDS             3800..5080
                     /locus_tag="PK348_02090"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HXK65033.1"
                     /translation="MKKSYLAFCDIEGNVYKHPGALAAFRSGRRFVKVDEKELIPIPD
                     GSTLYSLPKRYPVEGKNFKTITHYNGNTIWAVSSFLSSGYLRTYLPAYVKTKDAPVLS
                     LWAYCGLVFVGDDFYVPALRIDPDPRSDPALHRNDIELFKQIKVVKEQYPSNSLVRQL
                     AKCSTQYGCLCARNFFLSRHEAPLPTTKSCNALCLGCFSFQKDTGVHVSQYRLTRQPS
                     PQEIAEVMLHHIERVDRAVVSFGQGCEGEPLLRAKDLAAAISIVRSKTSKGTIHLNTN
                     GSLPQGLQQCIDAGLDSVRISMNSPTEEYYTRYYRPKNYSFKDMLRSIEVALKAGIFI
                     SINLFFLPGFTDAQSEVERLCTFLDNFPVNMIQTRNLNIDPDYYLDSIGFEDEEAIGV
                     RQLLKLLQTKYPRLTLGYYNPPKEEFGVITKVDW"
     gene            5123..5530
                     /locus_tag="PK348_02095"
     CDS             5123..5530
                     /locus_tag="PK348_02095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HXK65034.1"
                     /translation="MEKMVQDPIGYARDVVGKDPLATFLGIEVEDVKHGYARCGLTVK
                     PEYLNAVERAHGGIIHAVADQAFAVASNSMGTMAVALTMTLQYLQAALDGERIFAECM
                     LVHAGRKISSWRLEVRGNNDAIIAIGDAVAYHK"
     gene            5543..>5824
                     /locus_tag="PK348_02100"
     CDS             5543..>5824
                     /locus_tag="PK348_02100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65035.1"
                     /translation="MEDSAKRLEVIVEFMVPGATLSGDTFDSENNFLYPAYTVYTSEI
                     IDTLKAKGIKRIYYTPAENNDATAFTEQAQDIWESLFEAVRTNARPDLEQ"
BASE COUNT         1817 a         1029 c         1359 g         1619 t
ORIGIN      
        1 ttggcaattc cggtacatca ctgtattttg gtataggggt ggcttcactt atacatggaa
       61 tgacagtatt tacaggagat catcagatac gtaaccgccc agctgaagat ttactcaatg
      121 caattactga attaggtggg tacgctgttt ccacccgttc aaatggcaaa ccgccagttg
      181 tggtacaggg accaatcatt ggcggacatg tttcaatacg cgctattacc tctcaatacc
      241 tttcatcgct gctggtggca acaccaaagg caaagaacaa taccattata gatgtacctc
      301 tcctcaatga agctccctat gtgggaatga cgttgtggtg gcttaaagaa cttggcatag
      361 aatatcacaa tacaggttac gaacaattta ttgttaaagg cgggcaaaga tataaaccct
      421 tcaccaaata cattccggct gatttttcat cggcaacttt ttttatggta gcagcagcca
      481 taacgcaaag cagcgttgta ttaaaggggc ttgattttaa tgacacacag ggtgacaaag
      541 aagtagtgaa tattttgaag gcaatgggtg ctgacgttga tattatgccg gatgctattg
      601 ccattagtgg aaaaccattg aaaggaggtg tgtttgacct caatgcgata cctgatgcgc
      661 tgcctgcact ttcagtgtgt gcgtgttttg cagatggtga aacgcgcctt gtcaatgtgc
      721 cacaggcacg ggtaaaggaa actgacagga ttgcagtgat gtgtgccgaa ttaaaaaaga
      781 tgggtgcaga tattgaggaa ttgcccgatg gacttatcat acgaaaaagc acattgaaag
      841 gtgcacatgt taacggccat cacgatcaca gggtagtgat gtcactggct gttgcagggc
      901 ttgctgcaga gggtagcaca accatagata ctgcagaatc cgtatcagtt acatttccca
      961 attttgtaga gcttatgcag gcgttaggtg ccagcatacg caaggaggaa tgatggttgt
     1021 acgtggagtg cgtggtgcaa ttaccgttga cgaaaattca aaagaggaga tagttcatag
     1081 aactgaagag cttttacggg cgatagttac tataaataaa attcagacca atgatattgc
     1141 ctcagccatt tttactgtaa ccgatgatct gaatgcagaa tttccagcgg tagcagcgcg
     1201 taagttagga tggattcata taccgctgtt gtgttcccgt gagattccgg ttccgggatc
     1261 acttggtatg tgcgtgaggg ttttgctgca tatcaatact gataaaaatc ctgaagagat
     1321 agttcatgtg tatctgcggg atgcaaggaa attgaggcct gatataggaa caccagaaca
     1381 ggataaatat tatgtatcag atagccagac ataaaaaata atttgcaaat ataatgttgt
     1441 atgatgtatc cttattctat tgttttggct actttccata tgcggcatga aaaaagttta
     1501 cttattttaa aactgcgaaa gtataaaaag tgatatccgt aataatgtgt gccgggaaaa
     1561 tgcaatgaag aatatccaca gtataatgtt agtaatcctt cttggtgtca gtatcatctt
     1621 tttttcacat gatcatagtg ttgcccaggc tgatagagag atagaactga ccgcacagga
     1681 aatattagcc cgagttgatc gcattatgga ctatcctgac ggaattctta aagggtatat
     1741 gatgcatata acacctgatg ggtcatccca aaaattacaa gtaacagggt atatcaataa
     1801 gaatcagttt ttgtttgata ttgtaaaacc agcacgagga agtatgcaaa agatactgta
     1861 ccgtataggt ggtgaagacc tgtgggtata tgacatggct gcacgggcac ttttccataa
     1921 gatgggtatt gataaatatg atgatatact ggcaaccaac tttttttatg ttgatttatc
     1981 caatgcagat ttacagagta attatgttgc acgcatcgat ggttcggtta ttttgaaaaa
     2041 tcaggactgc tataagctga cactggatcc tattgataaa acgggtgcgt atggtaaact
     2101 tacattatat gtaatcaaac aaaattatat tccattacga atcgattatc acgatcagga
     2161 taaagtaata tttaagaccc tttcaattgc aaagattgga caaaaagatg ggaaatcatt
     2221 ccccatacgg tatgatatgc tgaatatcaa aaaaggcaca atgactgtac ttgaatttac
     2281 ttctatagaa gatggagcaa ctattaatac agaaatattc aggcctgaaa aattagggga
     2341 ttagcgtggc acagtttttt gtcagtgcgc cgggtcatga taatacagtg gttattgaag
     2401 gtaaggatta ctaccattta gtgcgggtac gtcgtgtacg tgttggtgat agtattgatg
     2461 tcattgacac agaaggcgta cgctacagag caactatcgc acacatagaa gcacaaaaaa
     2521 ttattgctac tataatgcag accattacat atccagaaat ttcattaaac ctgacattgt
     2581 gtgtagcgct attaaaaggc aaaaaatttg atcttgtcat tcaaaaagca gtggagatag
     2641 gggtacgtag cataattcct gttgtgactg aacgtaccat accggatatt tctgaaaaag
     2701 aagaaaaaaa gttagaacgc tggcaaaaaa ttgccactga agctgcaaag cagtgtttaa
     2761 gaaaagatgt tccccgggtc agttccgtga tggaatatac tgatgtgcta caattatcta
     2821 agggcattgg tatactggca catgcagatc ctgaagcaca gaatttacgg cagtatttaa
     2881 aaaataatcc gcagcataac gatgttatgc tgttaacagg ccctgagggt ggcttctcaa
     2941 aaaaagaggt tgaaattgca cgacaatttg gttggattgt attatataat ggagcaacac
     3001 agcttagagc tgaaacagca gctatagtat tacctgccat tattatatat gagtggggct
     3061 atacgtaaaa aaggaaacag tatgaggatc agggtaaacg gcatagatat tgaatttgaa
     3121 aaaaccacat taaaagagct tcttgatttg taccgattta atccctcaaa aatcatggtg
     3181 gagaagaatg gccgtattgt ggaaaaggaa aactatgcca ccgaaacatt acaggatggc
     3241 gatgtccttg aaatagcacg gtttgttggt gggggatgat agaaaaatta cgtactgctt
     3301 taatggaatc ctctctttca catgaggagt tgcaacacat tatgttcctg cttttttgtt
     3361 gcggcgatag taaccggcaa gtgcctgaag atttaatcaa agctggatat atatccataa
     3421 acgacaattg ctgcacgcta accgataaag gtagagcata ccgtgatagc ctgttacaga
     3481 agctggcaaa gtttcatgct gcaagtgatg aagttttcag gcaatatttt gataatgagc
     3541 tattgcccat taaaaaaaag atgcagttat taaaacacga gtggcgcagt acccgtgaca
     3601 aacggttgcg ctataaagaa accctgatga agcagggaaa ggataaagct gcaataaaaa
     3661 aagatgtaca atataaaaag ctacaaaggc agcagaatac actgcgcaca cgcatgcgcc
     3721 atgcagaaaa agaatataat aaaaaattat ctgcatgtat aaagcggatt ggtatacttg
     3781 atttgtaaag tgcaaaggta tgaagaaatc atatttagca ttttgcgata ttgagggtaa
     3841 tgtgtacaaa catccgggag ccttggcagc tttcaggtca ggccgccggt ttgtgaaggt
     3901 ggatgaaaaa gagcttatac ctatccctga tggcagtaca ttatactctt taccaaaacg
     3961 ctatcccgtt gaaggaaaga atttcaaaac aataacgcac tataacggca atacaatctg
     4021 ggcagtttca tcgtttttaa gttcagggta tttacgaaca tacctgcctg catatgttaa
     4081 aactaaggat gcacccgttc tttctttatg ggcgtactgt ggccttgtgt ttgtgggtga
     4141 tgatttttat gtgccggcac ttcgcattga tcctgatcca cgctctgacc ccgcattaca
     4201 ccgcaatgat attgaactgt ttaagcaaat aaaagtggtt aaagaacagt acccctcaaa
     4261 cagcctggtg cgccagcttg caaaatgctc cactcagtat gggtgcctgt gtgcgcgcaa
     4321 tttcttttta agccggcatg aagcaccgct gccaacaaca aagtcatgca acgcattgtg
     4381 tttgggctgc ttttcgtttc aaaaagatac tggtgttcat gtatcacagt acaggcttac
     4441 ccgtcagccc tcaccacagg agatagctga agtgatgcta catcacattg agagggttga
     4501 cagagcagtg gttagctttg gtcagggttg cgaaggtgag ccgctgttac gcgcaaagga
     4561 tttagcagcg gccatatcaa tcgtgcgaag caaaacttca aaaggtacca ttcacctgaa
     4621 caccaatgga tcattgcctc agggcttgca acagtgtatt gatgcagggc ttgattctgt
     4681 gcgcattagc atgaattcgc caacagaaga gtattatacc aggtactatc gcccaaaaaa
     4741 ttatagcttt aaggatatgt tgcgcagtat tgaggtggct ttaaaagctg gtatttttat
     4801 atcaataaat cttttctttt tacccggttt tactgacgca cagtcagagg ttgagcgttt
     4861 atgtactttt cttgacaact tcccggtgaa catgatacaa acccgtaatc tgaatattga
     4921 tccagattat tatctggaca gtattggttt tgaagatgaa gaagcaatag gtgtgcgaca
     4981 gttactaaaa cttttgcaaa caaaatatcc caggcttaca ttagggtatt ataatccccc
     5041 aaaagaggag tttggagtta tcacaaaagt tgattggtaa tatggtgtgt gataaaatta
     5101 atgaaaaatt ttgaggtgaa taatggaaaa gatggttcag gatcccattg gctatgccag
     5161 ggatgttgtg gggaaagatc ctctggcaac atttttaggg attgaagtgg aggatgtaaa
     5221 gcatgggtat gcgcgctgtg gtttaaccgt aaagccggaa tacctcaatg cggtagagcg
     5281 tgcacatggc ggtattattc atgctgtggc agatcaggca tttgcagtgg caagcaattc
     5341 catgggaact atggcggtgg ctcttaccat gacactgcaa tatttgcagg cagccctgga
     5401 tggtgagcgg atatttgccg aatgcatgct ggttcatgct ggcaggaaaa taagttcctg
     5461 gcgacttgag gtgcgtggga acaatgatgc cattatagct attggagatg ctgtggctta
     5521 ccataaataa ggtgttgcta gtatggaaga tagcgctaaa cgtttagagg ttattgttga
     5581 atttatggtt ccgggtgcaa cgctttccgg agatactttt gattctgaaa ataatttttt
     5641 atatcctgcc tatactgtgt ataccagtga aattattgat acgctgaaag caaaaggaat
     5701 aaaacgtata tattatacgc cagcggaaaa taatgatgca acggcattca ctgaacaggc
     5761 gcaggatatc tgggaatcat tgtttgaagc tgtcaggaca aatgcacggc ctgatctgga
     5821 gcaa
//
LOCUS       DAOSZP010000057         5768 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_11686_length_5768_cov_3.145458, whole genome shotgun sequence.
ACCESSION   DAOSZP010000057 DAOSZP010000000
VERSION     DAOSZP010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5768
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11686_length_5768_cov_3.145458"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(389..1087)
                     /locus_tag="PK348_02105"
     CDS             complement(389..1087)
                     /locus_tag="PK348_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010582603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HXK65036.1"
                     /translation="MLLGIDPLYWMMMAPVLLISLWASFRVKSAFNKYSQIPSRSGHT
                     GADVARMILQQNGLSHIPVEETTGFLSDHYDPIKKVVRLSPEVYNSNSLAAIGVAAHE
                     TGHAIQHAKLYRPLVLRNAIAPTASIGSNLSWIIIFAGFAMGSLGLVKLGILLFSLVV
                     FFQLITLPVEFNASSRAKEILQGYGIVSSGELAGVSKVLSAAAMTYVAAAASAIMTLL
                     YFLIRAGLLGGRDE"
     gene            1440..3296
                     /locus_tag="PK348_02110"
     CDS             1440..3296
                     /locus_tag="PK348_02110"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXK65037.1"
                     /translation="MKKFLPLLMIIAIACSSLPDTKTETIARPVYKETSFELYSKALL
                     LKNEKKYTEAIKLFEEASKEGTQLDAIYYQIAECYYYQYDYAKAIEYASKSIAVNENW
                     DKPYVLQYSVYMNLNQADEGVTVLEKLLQKRPELYRVRFNVATVYYSQLKQNVRARYH
                     FLKVIEQSECESIESYYKEQSYYYLGHIYYSFGEFKKAYHYFLKAYNENPENYSMLYI
                     LGLLNLEKGDIKQAISYIIQYLERYPNDLKAILHAGRIYYIYEDMKALPVLRKAINHR
                     SSEGLLARMMYNELLHNDDAARELAQKLNRMNTSLDMPYIALARIAMRKHDTATAAAN
                     FYTAGVMLYQKKDYEAATIMFTLALTNDPSIYEAYLYLGQLYEDTNQLNRAIYYYTIA
                     YSMKKENDVLLRIGYLYALLKDYTKAFEYMNAAQNQDKENPDAYFFKGLVYMQKNNYP
                     DAEDMLRKAVDLKKSETYYFYLASALDKQKRVQDTIAVLQEACNNEQKSARICNYLGY
                     LYADNNIRLDESLMLINRALESEPTNGAYLDSLGWVYFKMGKYHDALPLLLEAAQELE
                     LEGYPDAVVYEHIGDAYNALQLPDKALEYWNKAYKLDPKPEVQKKIDTHKGK"
     gene            3304..4095
                     /locus_tag="PK348_02115"
     CDS             3304..4095
                     /locus_tag="PK348_02115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65038.1"
                     /translation="MNVKSKNNCYIVVILSLVVLYGCTSVPVKETEKKETSATLLLEE
                     IIKLNATLPHTVIASLRIENISKEGFKSSAKLLYNVDARKAKMSIVDSVFGTQLSVVV
                     RDNDTLYIYQPIANNLLVFDYNKFPVYSFLPFNIDVELIDNLLIGKIPLLKNYYSVKA
                     LEADSEQFILLENDTMHQTISMKDNVPSKILFVVKSTNSRYEIYFLKPKIDNNMFTCK
                     ALRFVDVNGKTTFEVTMQQYMINEAVPDTVAELKLPKNTKITDYR"
     gene            complement(4086..5321)
                     /locus_tag="PK348_02120"
     CDS             complement(4086..5321)
                     /locus_tag="PK348_02120"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HXK65039.1"
                     /translation="MRICLLCYRANPFCGGQGIYLKYVAEAFARQGHEVHAIVGPPYP
                     HHMDHVTIHYIENNQYYIKKGVECITYDNPFDIFHPINAYEYIHSRLGAFPEISGFSY
                     RAFFMVRKLHEKLHFDVIHDNQCIGYGLLFMKALGIPVIATIHHPLTIDLENVLERAS
                     SFKNRMKGVMFYPILMQQIVSKRLDHIITVSEDSKHRITKDFGVPPHKQSVVYNGLDT
                     SIFRKLPNIKKKHNKLLFVGNVEDGKKGFVYLLKALTLIQSDATLTVIDGGAPHRRIT
                     QKLVDTLGMRNKVEFVGAASTDELVHHYNEATIAIVPSVYEGFGFPAAEAMACGTPVI
                     ASDGGALREVVGDAGIVVPARDEVALANAIDNLLQNKKLLQELSHKGIKRVQTHFNWD
                     NAALQMAAIYQKVIASYYR"
     gene            complement(5318..>5768)
                     /locus_tag="PK348_02125"
     CDS             complement(5318..>5768)
                     /locus_tag="PK348_02125"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020876666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HXK65040.1"
                     /translation="AANGSKTALTTVRLAGHEGRIDIGNNVLVMNGVRISSASHIVIG
                     DDCMLANFCYLTDADWHDIHDRTNPVGKTAPIILEKGVWIGDSAIICKGVRIGQNSVV
                     GAGAVVTKDVPPNVVVAGNPARIVKEIDPDKVVTHSTLYHKVGAPRI"
BASE COUNT         1855 a         1087 c         1115 g         1711 t
ORIGIN      
        1 tgcagtgacg tgcaaccaaa cgtcattgca agtcctgact tgtcactcat accgttatcc
       61 tgatgagtca cagctttttt gaaacgatga aggattttgc cgtttctcac gtaaaatgtc
      121 cgctttttct atgtgatgtg aaatcaacgt tattacagat cacaacgtac tatctactat
      181 gtcatcctga ggagtccctt tctttttggg acgacgaagg atctcgccgt tcaaatcatt
      241 aaatatttct aactctgtat tttgttacaa caccgcaggc actcaagcgc ttgagagcct
      301 gcaattttat ttttttatca attacatatc cttactctga cccaggtttt taatatgctc
      361 actccatctt atttcttata accttttttt attcgtctct accacctaag agtccagccc
      421 ttatgaggaa atacaacagt gtcattattg ctgatgctgc agcagccaca tacgtcattg
      481 cagcagcgga tagtactttg ctaacccctg caagctcccc tgaactcaca atgccataac
      541 cttgtaaaat ttcctttgca cgtgatgaag cattaaattc taccggtagt gtaataagct
      601 gaaagaatac taccaggcta aaaagcaaaa ttcccagttt aacaagccct aatgaaccca
      661 tagcaaagcc tgcaaaaata attatccatg aaaggtttga accaatggac gcggttggtg
      721 ctatggcatt acgcagaaca agaggcctgt acagttttgc atgctgaata gcgtgaccag
      781 tttcatgtgc tgccacacca attgcagcaa gtgaattgct attatatact tcaggtgaaa
      841 gcctgactac tttctttatg gggtcataat ggtctgataa aaaacctgtg gtttcttcaa
      901 ccgggatatg tgataatcca ttttgctgta aaatcatacg tgccacatct gctcctgtat
      961 gtccggatcg tgatggaatt tgagaatatt tgttaaatgc actttttacc ctgaacgaag
     1021 cccacagtga aattagaaga acaggtgcca tcatcatcca gtataagggg tcaattccca
     1081 aaagcatgtg ttacctcctg aaaagttttt aatgttctaa catattcctt actttcattg
     1141 aactgtatgt tgcctttaaa tgcatgcagt gtataatata caaaatattg aggcatttgc
     1201 aaagtatttt tgtatcaatc cttttgtttt tgttacaaaa ttattccatg acgatttata
     1261 tcgatgtttt atgtttgaca gttaccataa atagtatata ttgttatata cgttaatgtg
     1321 tgcatggtat tgtacacata cataggtatt atcgcatatc cccgacacca tatgcggaaa
     1381 attatactat ccattgtgta ccgggaaata ctaaaaggtt attaaaagga actatcgcca
     1441 tgaaaaaatt cttgccgctt cttatgatta ttgccattgc atgcagttct ttaccagata
     1501 caaagactga aactattgcc aggccagtat ataaagaaac atcgtttgaa ctgtacagca
     1561 aggcattact attaaaaaat gaaaaaaaat atactgaagc tatcaaactc tttgaagaag
     1621 ccagcaaaga aggtactcag ttagatgcta tttactatca gattgcagaa tgttattatt
     1681 atcagtatga ttatgcaaag gcaatagaat atgccagcaa atcaattgca gtaaatgaaa
     1741 actgggataa gccgtatgtg ttgcagtatt cggtatacat gaatctgaac caggctgatg
     1801 aaggcgttac ggttcttgaa aagctgttgc agaaaaggcc tgaactgtac cgtgtacgtt
     1861 ttaatgtagc aaccgtctat tattcacagt taaaacagaa tgtcagagcg cgatatcatt
     1921 ttttaaaagt tatagaacag agcgaatgtg aatcaattga atcgtactat aaagaacaat
     1981 catattatta tttagggcac atctattata gttttggcga atttaaaaaa gcgtatcact
     2041 attttttaaa agcgtataat gaaaatccgg aaaattattc catgttgtat atactggggc
     2101 ttctgaatct ggagaaaggt gatataaaac aggcaataag ttatattata caatatcttg
     2161 aacgatatcc caatgatctg aaagcaatat tgcatgcagg cagaatatac tatatctatg
     2221 aagatatgaa agcgttgcct gttttgcgga aagccattaa tcacagaagc tcggaaggat
     2281 tgctggcacg gatgatgtat aatgaactgc tgcacaatga cgatgcagcc agagagctgg
     2341 cacaaaagct taatagaatg aacacgtctc ttgatatgcc gtatattgca ctggctcgca
     2401 ttgcaatgag aaaacatgat actgctactg ctgcagcaaa cttttataca gcgggtgtta
     2461 tgctgtatca aaaaaaagat tatgaagctg caacaataat gttcacgctg gctttgacta
     2521 atgacccatc aatatatgag gcatatctgt atttaggaca attatatgaa gataccaatc
     2581 aactgaacag agctatctat tactatacca ttgcatattc catgaaaaaa gaaaatgacg
     2641 ttttattaag aattggctat ctgtatgcac ttttgaagga ttatacaaag gcttttgaat
     2701 atatgaatgc agcccaaaac caggataaag aaaatcctga tgcgtatttt tttaaaggtc
     2761 tggtgtatat gcaaaaaaat aattatcctg atgcagaaga tatgctcaga aaggcagttg
     2821 atttaaaaaa gtcggaaacc tattattttt acctggcatc tgctcttgat aaacagaaac
     2881 gagtacagga tactatcgcc gtattacagg aagcctgcaa caatgaacag aaaagtgcaa
     2941 ggatttgtaa ctatttagga tacttatatg ctgataacaa catacgcttg gatgaatcgt
     3001 taatgctcat taacagggcg ctggaaagtg aacctacaaa tggagcatat ttagattcac
     3061 tgggatgggt atatttcaaa atggggaaat atcatgatgc attgccttta ctgttagaag
     3121 cagcacagga attagagctt gaaggctatc ctgacgcagt tgtgtatgaa catattggcg
     3181 atgcctacaa tgccctgcaa ttgccggaca aagcactgga gtactggaac aaagcatata
     3241 aacttgatcc aaaaccggaa gtgcaaaaaa agattgatac tcataaaggg aagtaatagt
     3301 aatatgaatg tgaaaagtaa aaataactgc tacatagtag taatactatc tctggttgtt
     3361 ttgtatgggt gtacttctgt accagtaaaa gaaacagaaa aaaaggaaac aagtgcaaca
     3421 ctgctcttag aagaaataat aaaattaaat gctacattgc ctcatacagt gattgcatcg
     3481 ttgcgaatag aaaatatatc taaagaaggt ttcaagtcat cagcaaaact gctctataat
     3541 gtggatgcaa ggaaggcaaa aatgtcaatt gttgattcag tatttggaac tcagctgagc
     3601 gtggtggtgc gagataatga taccctttat atttatcaac caattgcaaa caatctttta
     3661 gtgtttgact ataataaatt ccctgtatat tcttttctcc catttaatat tgatgttgaa
     3721 ctaatagaca atctgttgat tggcaagata ccgcttttaa aaaattatta tagtgtaaag
     3781 gcgcttgaag ctgatagcga acaatttata ttactggaaa atgacacaat gcatcagact
     3841 atatctatga aagacaatgt ccccagtaaa attttgtttg tggtaaagag caccaacagc
     3901 cggtatgaaa tatatttttt aaaacctaag atagataata acatgtttac ctgtaaagca
     3961 ttacggtttg ttgatgttaa tggcaaaaca acatttgagg taaccatgca gcagtacatg
     4021 ataaatgaag cagtgcctga tactgtggct gagctaaagc tacctaaaaa tacaaaaata
     4081 acagattacc gatagtaact ggcaataacc ttttgataaa tagctgccat ctgtaacgca
     4141 gcattgtccc agttaaaatg tgtttgtaca cgtttaattc ctttatgcga tagttcctgt
     4201 aatagctttt tattttgcaa caggttatcg atggcattgg ccagtgctac ctcatcccgt
     4261 gcaggtacaa caatccctgc atcaccaacc acctccctta gtgcaccacc gtcgctggca
     4321 ataacaggcg tgccgcatgc catagcctcg gctgcaggaa atccaaatcc ttcatacact
     4381 gaaggaacaa tggcgatggt tgcttcatta tagtgatgta ccagttcatc agttgatgcg
     4441 gcaccaacaa attctacttt gttgcgcatg ccaagagtat caacaagttt ctgtgtgata
     4501 cgacggtgtg gagcaccacc atcaataacg gttagtgtgg catcactctg tatcagggtt
     4561 aaagccttga gcaaatatac aaatcctttc ttgccatctt caacattacc cacaaataaa
     4621 agcttattgt gttttttctt tatatttggc aattttctga aaattgaagt atcaagtcca
     4681 ttgtatacca ctgactgttt gtgtggagga acaccaaaat cttttgttat tctgtgtttg
     4741 gaatcctctg aaacagtgat gatgtggtca aggcgttttg acacaatttg ctgcataagg
     4801 atgggataaa acatgactcc tttcattctg tttttgaatg atgaagcacg ttctaaaaca
     4861 ttttctaagt caatggttaa tggatgatgg atagtggcta ttacaggaat gcccagtgct
     4921 ttcatgaata gcagtccata gccaatacac tgattatcgt ggatgacatc aaaatggagt
     4981 ttttcatgga gctttcgcac cataaaaaat gcgcggtaac tgaatccgct tatttcagga
     5041 aaggccccaa gccgtgaatg gatatattca taggcattaa tgggatgaaa aatatcaaat
     5101 ggattatcat acgtgataca ttcaacgcct tttttaatat agtactgatt attttcaata
     5161 tagtggatag taacatgatc catgtggtgg ggatagggag gaccaacgat ggcatgcact
     5221 tcatgcccct gccgtgcaaa agcttctgct acatatttaa ggtatatacc ctgaccaccg
     5281 caaaaagggt tggcacgata acacaaaagg catatcctca tatacgaggc gctcccacct
     5341 tatgataaag agtactgtgt gtcacaactt tatcgggatc aatttctttt acaatccgtg
     5401 caggattgcc agcaacaacc acattgggtg gcacatcttt ggttactacg gcacctgcac
     5461 caactactga gttctggcca atgcgcactc ctttacagat aatggctgaa tcgccaatcc
     5521 ataccccttt ttcaaggata ataggtgcag tcttgcccac agggttggtg cggtcatgta
     5581 tatcgtgcca gtcagcatcg gtaaggtagc aaaagttagc taacatgcag tcatcgccaa
     5641 taacgatatg tgaggcactg cttattcgca cgccgttcat cacaagtacg ttattcccta
     5701 tatcaatacg cccttcatga cccgcaagtc gtacggttgt taaggcggtt ttactgccat
     5761 tggcagct
//
LOCUS       DAOSZP010000058         5703 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_11867_length_5703_cov_3.372698, whole genome shotgun sequence.
ACCESSION   DAOSZP010000058 DAOSZP010000000
VERSION     DAOSZP010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5703
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11867_length_5703_cov_3.372698"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="PK348_02130"
     CDS             complement(<1..264)
                     /locus_tag="PK348_02130"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HXK65041.1"
                     /translation="MLSDYHYNTHTVLDLFKDGVKKYGNKVLLNHHNGTQWESFTWNQ
                     VDTMVKALASYFIDLGLKPGDVVSIYSENRPEWAIADLATLSIG"
     gene            759..>2298
                     /locus_tag="PK348_02135"
     CDS             759..>2298
                     /locus_tag="PK348_02135"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HXK65042.1"
                     /translation="MKVLLINPPYPFEESPSPPFGLMALAAYLEEKNIEVKIEDYIVN
                     RFSENRAQDVMSSFAPDVVGSTGVTMNIKKALAILKVYKQINPDIITVLGGPHATFDA
                     HNMLLHHPYVDVIVRGEGELTFEQLLSHLNNKNAYAEIEGISYRDNDNTIVHNPNRDF
                     IPDINILPYPARHLVELSKYRALGFPINMMTSRGCPFNCIFCVGSKMVGRKVRYFETN
                     RVVDEFAMLATMKFKQINIVDDLFTSHKSRCIEVCKEIKRRGINHPWTAFARVDTVNI
                     ELLEHMKDAGCTMLCFGIESGNQEILDKVKKKITLEKCKQAVAMCRQVGISPMTSYIL
                     GLPGETEETIKKTLEFAKELSDNYGFHVLAPFPGTEVREMKDYYGIEILTNDWDLYDA
                     NRAVSVTPWVTADRINEIVDRFNGSIKAYILSLGEKQNHGQKLSHSEQELISNIRSFE
                     IVRDMILDRLIEEFPGIENGVPVDVKKEFVEYLSQKTKFSKEELQNQVERLVELQCID
                     FEYRN"
     assembly_gap    2299..2308
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2479..2958
                     /locus_tag="PK348_02140"
     CDS             2479..2958
                     /locus_tag="PK348_02140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65043.1"
                     /translation="MNFETTTCISKEHYTLLCYYSEKYNVKLSKLITALLRFVGENQK
                     VYFTTNRRIKYRERQGGNWKRIHIMLTPYDYEIILDLKKYGKMSLAKMIEFSIENYFE
                     RFVNRVLKKQSTDNYLYANYHFEVTTENNIFSYSVYWGLPPKILQKLTKTTHLFSKT"
     gene            complement(2972..3781)
                     /locus_tag="PK348_02145"
     CDS             complement(2972..3781)
                     /locus_tag="PK348_02145"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXK65044.1"
                     /translation="MIVRDKNFLQDYIKVWDDGAIKDAPFITLGLEAVLTDYYGNLLD
                     APPPFEVKPDETILDLGCGWGRVLKPVRAITDNAIGLDISHQMLMAAQKHLHHHNLPT
                     PLVRGDGTLLPFKDCSFDTVYSLLVLQHLSKENGKKVIKEIHRILKPGGTAYIRVPSR
                     FSPENLLMAFLQLVSIHIFRYKDPIRMRFYRIGEIHKICKPLFNTVEITAHEFRPPWN
                     FHTPWTWHYIIIPKRFHKRLRVLSDKLEKRANSKSPWLKHFGVTLMIKVVK"
     gene            complement(3765..5249)
                     /locus_tag="PK348_02150"
     CDS             complement(3765..5249)
                     /locus_tag="PK348_02150"
                     /inference="COORDINATES: protein motif:HMM:NF024598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HXK65045.1"
                     /translation="MTMAYKGKIVKLVIVLSILLVIIFQWHRLIYIANYIKGYYITIK
                     DSLFAPDTVSIIYPTRFFNYKRPYHHAPHELLHCGNMLLYIAPKEEQVADIAQRLIGY
                     TYYYRASHLHNAIVTLNALTSKTVGAGSVLVIPHPLPFITIDLSKKSMTQIPYVKGVY
                     YSGNSLASSKMMRQLSELKQAGINAVVFDVKDVCGIVNYKSHVPIVVELNTHEKRPVD
                     DVVTLIRNFKELDLYVIARIAVFRDHLLATKNPDMAIKSKRTGGIWNQGSKELWVDPT
                     KQTVWDYNIALACEIATLGADEVQFDYIRFPTAGDLSDAQYAYDFGKKTKVETITEFL
                     AKAKNELHKLNTAVSIDIFGVAAWQKEEDVSKLGQKIELLARSCDVISPMLYPSHFND
                     NFDGYANPGDEPYYFIATGNKKLQAIAPKLLIRPWLQAFKWRVSNYNEDYILAQVNAC
                     INTNTYGYLFWNASNDYTVVYKAFMKKNNGNAKKQRGKAGDSQR"
     gene            5340..>5703
                     /locus_tag="PK348_02155"
     CDS             5340..>5703
                     /locus_tag="PK348_02155"
                     /inference="COORDINATES: protein motif:HMM:NF014460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXK65046.1"
                     /translation="MVQIIKKNIFIIVQKVDGIIKGIADFNAYMKALVRSFRSLRYLK
                     IRSLYTIAINQTRFTGIDALPLVSWIALLLGATVIIQATTNFPKYGIESFIGNLLVII
                     IARELGPLVTALIVVARSG"
BASE COUNT         1781 a          965 c         1116 g         1831 t
ORIGIN      
        1 accaattgaa agagtggcta aatcggcaat tgcccattca gggcgatttt ctgaatagat
       61 tgatacaaca tctccaggtt ttaatcccag atcaatgaag tacgatgcca gggctttaac
      121 cattgtatca acctgattcc aggtaaagga ttcccactgt gtgccattat gatggtttaa
      181 taagacttta ttgccatatt tcttaacacc gtctttaaaa aggtccaaaa cagtatgtgt
      241 attgtaatga taatctgaca gcatatgtaa cctcaccatt tttttagaat aatagtattg
      301 ttgaaagtgt acgaatagta tatcatattg tcaattaaaa tatataaatt ttaataaata
      361 tacatgaaaa aatgataatt caattgtatc aggctatcac atgcaaatgt tagcatgtta
      421 gtatgtacat aaaataaggt gtgaaaaagc atcacgcaga gtgcgctgag atcgcaaaaa
      481 gcgctgtggg aaggagcagt gttatgtcgg aaattacaaa acttcgtatt ctaagcttta
      541 tctgtgtgaa aaaatatatt acgtagagtg tgcggatgtt actgtaggtg ctgaggaaag
      601 gggtcagagc atatgtttga aatcactatg ttcactccgt tttcagtgtg aggaaaagga
      661 gtaaacataa tttttttaaa aaaatattgc taaataatgt tcgatttctt aatgtcatta
      721 tttgaacatt cactattaca agtaataaaa ggaacattat gaaggtactg cttataaatc
      781 caccataccc ttttgaggaa tcgccatcac caccatttgg acttatggca cttgctgcat
      841 atcttgaaga aaaaaacatt gaagtaaaaa ttgaggatta tatagtcaac aggttttcag
      901 aaaaccgtgc acaggacgtt atgtcatcat ttgcaccgga tgttgttggt tcaaccggag
      961 ttacgatgaa cataaagaag gcgttagcaa ttttgaaagt gtataaacag attaatccag
     1021 atatcatcac agtacttgga ggacctcacg caacgtttga tgcacataat atgttacttc
     1081 atcatccgta tgttgatgtc attgtgcgtg gtgaagggga attgactttt gaacagttac
     1141 tatcgcacct gaataataaa aatgcatatg cagaaattga aggcatttca taccgcgata
     1201 atgacaatac tattgttcat aatcccaaca gggattttat acctgatatt aatatacttc
     1261 cttaccctgc acggcattta gtggagcttt caaaatatag agcgctgggt tttcccatta
     1321 acatgatgac atcacgggga tgcccgttta attgtatttt ttgtgtaggc agcaagatgg
     1381 ttggcaggaa ggtgcgctat tttgaaacaa atcgtgttgt tgatgaattt gctatgcttg
     1441 ccaccatgaa atttaaacaa attaatattg ttgatgacct ttttacatca cataaaagcc
     1501 gttgtattga agtgtgcaag gaaattaaac ggcgcgggat taatcatccg tggactgcgt
     1561 ttgctcgcgt tgatacggta aatattgagc tacttgaaca tatgaaagat gctggttgta
     1621 ccatgctttg ttttgggatt gaaagtggta atcaggaaat tcttgataaa gttaagaaaa
     1681 agataacact tgaaaagtgt aaacaggcag ttgcaatgtg caggcaggtt ggcattagcc
     1741 ctatgacgtc gtatatactg ggattgcctg gcgaaacaga agaaaccatt aaaaaaaccc
     1801 tggaatttgc aaaagagtta agcgataatt atggatttca tgtgttagca ccattccctg
     1861 gcactgaagt acgggaaatg aaagattatt atggtattga gattttaacc aatgactggg
     1921 atctgtatga tgctaacaga gctgtttcgg ttacgccctg ggttactgct gataggatta
     1981 atgagatagt tgatagattc aacggcagca taaaggcata tatattatcg ttgggtgaaa
     2041 agcagaatca tggccagaag ctttcacaca gtgaacagga actaatcagc aatatacggt
     2101 catttgaaat agttcgtgac atgatattag ataggcttat tgaagaattc ccgggtatag
     2161 aaaatggggt tcccgttgat gttaaaaagg aatttgtgga gtacctgtcg caaaaaacaa
     2221 aattttcaaa ggaagaatta caaaatcagg ttgagcggct tgtagagtta caatgtattg
     2281 attttgaata cagaaatann nnnnnnnnat aaaatttatc cggtgtggac ttaatatgag
     2341 tgatggagct actatagttt tttaggtttt tatttaccat caatagtaat taccatgagt
     2401 aattatcgtg tatatatttt gtgttgtaca atatatttat aaaatattac atatagaaaa
     2461 aagtatagcg gaggatatat gaattttgaa acaaccacat gtatatcaaa agagcattat
     2521 acactattat gctattattc agaaaagtat aatgtgaagc tctcaaaact tattactgct
     2581 ctgttacgtt ttgttggtga aaatcaaaaa gtttatttta ccacaaacag acgaattaaa
     2641 tatagggaaa gacagggagg taattggaaa cgaattcata ttatgcttac cccctatgat
     2701 tatgagataa ttctggattt gaagaagtat gggaaaatgt ctctggccaa gatgattgaa
     2761 tttagtattg aaaattattt tgaaagattt gttaacaggg ttcttaaaaa acaaagtacc
     2821 gataactatc tatatgcaaa ttatcatttt gaagttacaa cagaaaataa tatattcagt
     2881 tacagtgtct attggggatt accaccaaaa attcttcaaa agttaacaaa aaccactcat
     2941 ctattctcca aaacgtgacc ttaattgttt cttactttac tactttgatc atcagtgtta
     3001 caccaaagtg cttgagccat gggcttttac tgtttgctct cttttctagt ttgtccgata
     3061 gcactcgcag ccgcttgtgg aagcgtttgg ggatgataat atagtgccat gtccatggtg
     3121 tgtggaagtt ccatggtggt ctaaattcat gtgcagttat ttctacagtg ttgaacaggg
     3181 gcttacagat tttatgtatt tcaccaatac ggtagaagcg cattctgata ggatctttat
     3241 acctgaatat gtggatgctt acaagttgca aaaatgccat caacagattt tcaggagaaa
     3301 accgcgaagg cactcttata tatgctgttc ctccaggttt taatatgcgg tgtatttctt
     3361 ttataacttt tttgccgttt tctttggaaa gatgttgtaa taccagaagt gaatagactg
     3421 tatcaaagct acaatcctta aatggtaata acgtaccatc gcccctgacc aatggtgttg
     3481 gaagattgtg atggtgtaga tgtttctgtg ctgccatgag catctggtga gagatatcga
     3541 gaccaatggc attgtcagtt atagccctga caggctttaa cacacggccc caaccacaac
     3601 ctaaatccag tattgtttca tcaggtttta cttcaaaagg tggtggagca tctaaaaggt
     3661 ttccgtagta atcggtcagt acagcttcaa gccccaatgt gataaatggt gcgtccttaa
     3721 tagcgccatc atcccacact tttatataat cctgcaaaaa attcttatct ctgactatca
     3781 cctgcttttc ccctttgttt tttagcattg ccattattct ttttcataaa agctttatac
     3841 actaccgtat agtcattgga tgcattccag aataggtacc cgtatgtatt ggtgttgatg
     3901 catgcattaa cctgcgctaa tatatagtct tcattataat tgctgacacg ccatttaaat
     3961 gcctgtaacc atgggcggat taatagttta ggagctatcg cctgtaactt tttgttccct
     4021 gttgcaataa agtaatatgg ttcatcccca gggtttgcat atccatcaaa attgtcattg
     4081 aagtgtgaag ggtacagcat tggtgagatt acatcacagc ttcgtgccag tagttcaatt
     4141 ttttgcccta actttgatac gtcttcttct ttctgccatg cagcaacgcc aaaaatgtcg
     4201 atagaaactg cggtattgag cttatgcaat tcatttttag cttttgctaa aaattcagtt
     4261 atggtttcca cttttgtttt tttaccaaaa tcatacgcat actgtgcatc tgacaggtcc
     4321 cctgcggtag ggaaacgtat atagtcaaat tgtacttcat cagcacccag tgtagcaatt
     4381 tcgcaggcca gtgctatatt ataatcccat acagtctgtt ttgttgggtc aacccataat
     4441 tcctttgatc cctgattcca tattccaccc gtgcgttttg actttattgc catatcaggg
     4501 ttttttgttg ccagaagatg gtcacggaat acagcaatgc gtgcaatgac atacaaatca
     4561 agttccttga aatttcgtat gagcgtaacc acatcatcaa cagggcgctt ttcatgtgta
     4621 ttcaattcta ctactatggg tacatggctt ttataattta ctatgccgca tacatcttta
     4681 acatcaaaga caactgcgtt tattcctgcc tgttttaact cagaaagctg ccgcatcatt
     4741 ttgctgcttg ctaaactgtt accagaatag tagacccctt ttacataggg tatttgcgtc
     4801 attgattttt tggaaaggtc tatagttata aacggcaaag gatgtggtat cacaagaact
     4861 gaacctgcac caactgtttt agaagttaag gcattgagcg tcacaattgc attgtgaaga
     4921 tgtgatgccc tgtaatagta ggtataccca atcaaccttt gtgcaatgtc agcaacctgt
     4981 tcttcttttg gtgctatata gagaagcata ttgccacagt gtaaaagctc atgtggcgca
     5041 tggtgatagg ggcgcttgta attgaaaaag cgtgttggat agataatgga aaccgtgtca
     5101 ggtgcaaaaa gtgaatcttt aatggttata taatatcctt taatataatt tgcaatatat
     5161 ataagcctgt gccactggaa gattataaca agaagaatac tcagaacaat aacaagcttg
     5221 acgattttac ccttatatgc catagtcata ctattcactt tcaaaagata atgtcttact
     5281 atcacagtgt gtatcacact gtaaaaacaa ggaaataaat tcaatataaa aattattcta
     5341 tggttcagat tattaaaaaa aatattttca ttattgtaca aaaggttgat ggaattataa
     5401 agggtattgc tgatttcaat gcatatatga aagctcttgt tcgttctttt cgttcattgc
     5461 ggtatcttaa aatcagatca ctatatacca tagctattaa tcaaacacga tttacaggca
     5521 tagatgctct gccacttgtt tcgtggattg cgcttttact tggagcgaca gttattattc
     5581 aggcaactac caatttcccc aaatatggaa tagaaagctt tattggcaat ctattggtta
     5641 ttatcattgc tcgtgagctt ggtccactgg ttaccgcatt aattgtggta gcacgatctg
     5701 gtt
//
LOCUS       DAOSZP010000059         5637 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_12047_length_5637_cov_3.275170, whole genome shotgun sequence.
ACCESSION   DAOSZP010000059 DAOSZP010000000
VERSION     DAOSZP010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5637
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12047_length_5637_cov_3.275170"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            126..314
                     /gene="rpmD"
                     /locus_tag="PK348_02160"
     CDS             126..314
                     /gene="rpmD"
                     /locus_tag="PK348_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002705987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HXK65047.1"
                     /translation="MAKKLRVKQIRSYISAKPKQKATLRALGLRKINAERVHDDTPVI
                     RGMLQVVKHMVEVSEINE"
     gene            335..772
                     /gene="rplO"
                     /locus_tag="PK348_02165"
     CDS             335..772
                     /gene="rplO"
                     /locus_tag="PK348_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HXK65048.1"
                     /translation="MQYTLKKPEYIKKKKRIGCGPGSGHGKTSCKGHKGQKARSGAHF
                     KPGFEGGQMPLQRRVPKRGFNNAQFKEEVQIININDIAKLGIQEVTPEVLYQKGLIKS
                     QLLPVKLLANGDIASAVKVTVDAVSSSAKEKIEKAGGSVSLRS"
     gene            799..2145
                     /gene="secY"
                     /locus_tag="PK348_02170"
     CDS             799..2145
                     /gene="secY"
                     /locus_tag="PK348_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HXK65049.1"
                     /translation="MPNVITNIFKIPELRKRVLFTLFILVVYRIGAHIPIPGVNIEAL
                     SSYFEMAAKSHGGGFVDFFDLFAGGALKRFTIFALGIMPYISASIIMQLLTVVIPAIE
                     KLSKEGPEGYKKINQYTRYGTVLLCALQGFGLTMWMKSMTSPKGTVVPQDAGIGFIIV
                     AVLAITTGTMILMWLGEMITERGIGNGISLIIYAGIVVRAPSAFLDTMNRIISRSDPI
                     IALILIALFIGVVAASILLTLGVRKIPVQYGKRVVGRRMVQMASQAIPLRVNAAGVIP
                     IIFASSVILFPAQISNMLGATKYAIFTKMQYWLSPGHTAYMIIYSLLIIFFCYFYTAI
                     QFNPNDLAENLKKYGGFIPGIRPGQNTADYIMHILNRITLPGSIFLALIAIVPDFIIR
                     IWPEEVSPGMAYLFGGTSLLIIVGVALDTLKQIESQLLMRHYDGFFKKGRIKGRRY"
     gene            2193..2945
                     /gene="map"
                     /locus_tag="PK348_02175"
     CDS             2193..2945
                     /gene="map"
                     /locus_tag="PK348_02175"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HXK65050.1"
                     /translation="MAIFIYSEEEIKRVEDACRITAAVLDEIGKHVDAGITTFELDRI
                     ARDLIKKFKAIPAFLGYKGYPAAICTSINEVVVHGIPSKGQILKKGDIVGIDIGVQYN
                     GYFGDAAKTFVVGKVSNNVKKLLEVTREALERGILQCKDGNHLTDISHAIESHVVQYG
                     FQPVHEFVGHGIGRNLHEEPSIPNFGKPGKGPVIKTGMIFAIEPMINEGTYQVEILQD
                     GWTAVTKDRKFSAHFEHTVAVVNGNPKILTHN"
     gene            2974..3192
                     /gene="infA"
                     /locus_tag="PK348_02180"
     CDS             2974..3192
                     /gene="infA"
                     /locus_tag="PK348_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020675823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HXK65051.1"
                     /translation="MAKEEPIEVEGEVVEALPNAMFRVKLDNGHVVLAHISGKMRMHY
                     IRILPGDRVTVELSPYDLSRGRITYRSK"
     gene            3216..3344
                     /gene="rpmJ"
                     /locus_tag="PK348_02185"
     CDS             3216..3344
                     /gene="rpmJ"
                     /locus_tag="PK348_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013637834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HXK65052.1"
                     /translation="MKVRVSVKKICSDCKVIKRHGVVRVICKNPRHKQRQKKRTAK"
     gene            3363..3737
                     /gene="rpsM"
                     /locus_tag="PK348_02190"
     CDS             3363..3737
                     /gene="rpsM"
                     /locus_tag="PK348_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HXK65053.1"
                     /translation="MARIAGVDLPNNKRVEVALTYIFGIGPSSSRKILEDAQVNPDTR
                     VKDLTDEEINQLRKIIERSYKVEGDLRTQIAMDIKRLMDIGCYRGIRHRRGLPVRGQN
                     TKNNARTRKGNRKVSVGSKKKK"
     gene            3761..4147
                     /gene="rpsK"
                     /locus_tag="PK348_02195"
     CDS             3761..4147
                     /gene="rpsK"
                     /locus_tag="PK348_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HXK65054.1"
                     /translation="MKQKRTKKQEKKNIPVGKAFIQATYNNTIITITDMQGNVVSWAT
                     AGGEGFRGSRKSTPFAAQMAAKAAAQKAIDNANMRTIEVYVKGPGIGREAAIRSLYQA
                     GLNISKIKDITPIPHNGCRPRKRRRV"
     gene            4164..4793
                     /gene="rpsD"
                     /locus_tag="PK348_02200"
     CDS             4164..4793
                     /gene="rpsD"
                     /locus_tag="PK348_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011734648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HXK65055.1"
                     /translation="MAKYTGPLCKLCRREMTPLMLKGRRCLTDKCAIKHKKYPPGPPR
                     KRRTKLSDYGVQMREKQKIKRSYMLLETQFKRTFDEATRLKGVTGENMLVLLERRLDN
                     VIYRLGFASSRLQARQFVQHGHVIVNGKRVDIPSYQVKVNDIVEIKEDFKTNTMLEDA
                     IKLSKAVGSVPQWLEVDYEKRSGKIIRLPERKDIEPIFNEQVVVELYSK"
     gene            4834..>5637
                     /locus_tag="PK348_02205"
     CDS             4834..>5637
                     /locus_tag="PK348_02205"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002974165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HXK65056.1"
                     /translation="MSHKNLLKGFRRPSKIVFEHDEVNQSYGRFIAEPFERGYGLTIG
                     NSLRRVLLSSIEGAAITAVKIQGVPHEFTAIDGVVEDVTRIILNLKKLRFKYESELPK
                     VLHIVKDGEAELTGADFAIDSDVEIMNPETHIATIERGGRIDMEVQIERGRGYLPGEI
                     SKSSVETIGVIPIDAIFSPIRKVNIKVEDTRVGQRTDYDKLIIEIWTDGSVKPEDALA
                     QAAKIIKDHMTIFINFEEEVEEEEVAVDESHDKLKVILAKSIDELEFSVR"
BASE COUNT         1843 a          863 c         1344 g         1587 t
ORIGIN      
        1 cacgaaaccc ggttaatgtt accaaagcaa cgataaatgg cttattgcaa ttacgaagcc
       61 tgaaggacgt agctgattta cgtgaagtac cgttaccaaa gttatggcaa ccataagagg
      121 tgtatatggc taagaaatta cgtgtaaaac agatacgaag ttatataagt gcaaaaccaa
      181 agcaaaaagc aaccctgagg gctttaggat tgcggaaaat aaatgcagaa agagtacatg
      241 atgatactcc tgtaatcagg ggaatgcttc aagttgtaaa acatatggtt gaagtttcag
      301 agattaacga ataaaagtag taggtgtgag tgttatgcaa tatacattaa aaaaacctga
      361 atatataaaa aagaaaaagc gtattgggtg tggccctgga agtggtcatg gaaaaacaag
      421 ctgtaagggg cataaagggc aaaaagccag gtccggtgct cattttaaac cgggttttga
      481 aggtgggcag atgcccttgc agagaagggt tccaaaacgt ggatttaaca acgcacaatt
      541 taaggaagaa gtgcaaatta ttaatattaa tgatatagca aagttaggga ttcaggaagt
      601 aactcctgaa gttttgtacc agaagggtct gatcaaatca cagttgttgc ccgtgaaact
      661 gcttgctaat ggggatatcg cttcagcagt taaagttact gttgatgcag tttcatcttc
      721 tgcaaaagag aaaattgaaa aggccggtgg aagtgtttcg cttcgatcat aattatataa
      781 agaggtcata aagaaataat gccaaatgta attacaaata tattcaaaat tccagaactg
      841 cggaaaaggg tgttatttac cctttttatt ttggtagtat atcgtattgg tgcacatata
      901 ccaataccag gggtgaacat tgaggcgtta agttcatatt ttgagatggc tgcaaaaagc
      961 catggtgggg gatttgttga tttttttgac ctttttgcgg gtggcgcgct gaaacgcttt
     1021 actatatttg ccctggggat tatgccgtat atttctgcat caattataat gcagctttta
     1081 acagttgtta ttccggccat agagaagtta tctaaagaag gacctgaagg atataagaag
     1141 ataaaccagt atacccgtta tgggactgtg cttttgtgtg cgctgcaggg ctttggcctc
     1201 acgatgtgga tgaagtccat gacatccccc aagggtacag ttgtgcctca ggatgccggc
     1261 attggcttca ttatagttgc agtacttgct attactaccg gaaccatgat acttatgtgg
     1321 cttggcgaaa tgataacaga acgaggcatt ggtaatggta tatcgctaat catttatgca
     1381 ggtattgttg ttcgtgctcc ttcagcgttt cttgatacca tgaacaggat aatcagcaga
     1441 agtgacccta ttattgcgct catactgatt gcattattta ttggggtagt ggccgcatca
     1501 atactgttaa cgctgggtgt acggaagatt ccagtacagt atggcaaacg tgttgtggga
     1561 aggcgcatgg tacagatggc atcgcaggct atacctctgc gagtaaatgc ggcaggcgtt
     1621 atcccaatca tatttgcttc ttcagttatt ctttttccag cacagataag taatatgctg
     1681 ggtgcaacaa aatacgctat ttttaccaag atgcagtact ggttatcacc agggcatacc
     1741 gcatatatga ttatatacag cttgttaatt atctttttct gctattttta tacggcaata
     1801 cagtttaacc caaatgatct ggccgaaaat ttgaaaaaat acggtggatt tataccgggc
     1861 atacgaccag ggcagaacac tgctgattat attatgcata tcttaaacag gattacgtta
     1921 ccaggttcca tctttttggc actcattgcg atagttcctg attttattat acgcatatgg
     1981 cctgaagagg tatcaccggg aatggcatac ctttttggtg gcacatcatt attgattatc
     2041 gttggtgtgg cgctggatac attaaagcag atagagtcac agcttttaat gagacattat
     2101 gatggcttct ttaaaaaagg gcgtataaaa ggtagaagat attaaattta aaagctatat
     2161 ttatttttta tacaattata ttttgaggcg ttttggctat ttttatttac agtgaagagg
     2221 agatcaagcg ggttgaggat gcatgcagaa taactgctgc tgttctggat gagattggga
     2281 agcatgttga tgcaggtatc actacttttg agctggacag gattgcaagg gatttaataa
     2341 aaaagtttaa ggcaatcccg gcatttttag ggtataaagg gtatcctgca gctatctgta
     2401 catcaattaa tgaggttgtt gtccacggga tacccagtaa agggcaaatc ttaaaaaaag
     2461 gcgacatagt tggtattgat ataggcgttc agtataatgg atattttggt gatgcagcaa
     2521 aaacatttgt tgttgggaaa gtaagtaata acgttaaaaa attgcttgaa gttacaaggg
     2581 aagcacttga acgtggtatt ttacagtgta aagatggcaa tcatctgact gatatatcgc
     2641 atgctattga atcacatgta gttcaatatg gttttcagcc tgttcatgag tttgttggcc
     2701 atggtattgg aaggaatctg catgaagaac cttcaatacc caactttggc aaacctggca
     2761 aaggtcctgt gataaaaaca gggatgatct ttgcaataga accaatgatt aatgaaggca
     2821 catatcaggt tgagattttg caggatggat ggacagcagt aacaaaggac agaaaatttt
     2881 cagcacattt tgaacatact gttgctgttg ttaatgggaa tccaaaaata ttaacacaca
     2941 attaatttta tagtataagg agtttaaaac tggatggcca aagaagaacc gattgaagtt
     3001 gaaggtgaag tggttgaagc gcttcccaat gccatgttca gggttaaatt agataatggt
     3061 catgtagtat tagctcacat atctggcaaa atgaggatgc actatatacg aattttaccc
     3121 ggtgacagag taacagtaga gttgtcgccg tatgatttaa gcagaggaag gataacctat
     3181 cggtccaaat gatagttaat gtttgaggta tagatatgaa agtcagagtt tcggtaaaaa
     3241 agatttgcag tgattgtaaa gttataaagc gtcatggtgt tgtacgtgtg atctgcaaaa
     3301 atccacgtca taaacaacga cagaagaaaa gaacagcgaa ataatatgag gtagaggtat
     3361 atatggcacg aattgcaggt gttgatttac caaacaataa gcgtgttgaa gttgcgctta
     3421 cttatatttt tggtattgga ccttcatcat caaggaaaat tttagaggat gcacaggtta
     3481 atcctgatac tcgcgtaaag gacctgactg atgaagagat taatcagttg cgaaaaatca
     3541 ttgaaagaag ttataaggtt gaaggtgatt tgcgaaccca gatagcaatg gatattaaga
     3601 ggcttatgga tattggttgc tacaggggta tacgacacag gcgaggattg ccggtgcgag
     3661 gacagaatac caagaacaat gcacgtaccc gcaaagggaa cagaaaagta tcggttggaa
     3721 gcaaaaagaa gaaatagtaa gtcaatgata gaggtgtaca gtgaagcaga aacgaacaaa
     3781 gaagcaggaa aagaagaata taccagtagg taaagcgttc attcaagcta cgtataataa
     3841 tactattatt acaataacag atatgcaggg taatgttgta tcatgggcaa cagcaggtgg
     3901 agaaggattc cgtggttcgc ggaaatccac gccgtttgca gcacagatgg ctgcaaaagc
     3961 tgctgcgcag aaagcaatag ataatgcaaa tatgcgaacg attgaggtat atgtaaaagg
     4021 tcctggtatt ggacgtgaag cagctatacg ttccttatat caggctggtt taaatatctc
     4081 aaagattaaa gatattacac ccataccaca taatgggtgc cgaccacgca aacgaagaag
     4141 agtataatgt gagggtttag tttatggcaa agtatacagg gcctttatgt aaattatgta
     4201 gacgagaaat gactccctta atgcttaaag ggaggcgttg tttaaccgat aaatgtgcca
     4261 tcaagcataa gaaatatccc ccgggacctc cccgaaagag gagaaccaaa ttatctgact
     4321 atggcgtaca gatgcgtgaa aagcagaaga ttaagcgcag ctatatgctt ttagaaaccc
     4381 agttcaagcg tacgtttgat gaagctacac gccttaaagg tgttacaggc gagaatatgc
     4441 tcgttctgct tgagcgaaga cttgataatg ttatttatag attgggcttt gcatcttcac
     4501 ggttacaggc acgacagttt gttcagcatg ggcatgtaat tgttaatggc aagcgtgttg
     4561 atatcccttc ttatcaggtt aaggtaaatg atattgttga gatcaaggaa gactttaaaa
     4621 ccaatacaat gctggaagat gctatcaaat tgtcaaaagc agtgggatca gttcctcaat
     4681 ggctggaagt tgactatgag aagaggagtg gcaaaataat acggttacct gaacggaaag
     4741 atattgagcc gatatttaat gaacaggtgg ttgttgagtt atattctaag taagttatgt
     4801 ggttaagtat gtgtttattt attggaggta ataatgtccc ataaaaacct attaaagggt
     4861 tttcgaagac catcgaaaat tgtgtttgaa catgatgaag ttaatcaaag ttacggaaga
     4921 tttattgctg aaccttttga acgaggatat ggtttaacta tagggaattc tttacggcga
     4981 gttttattat catcgattga aggtgctgcc attactgcag taaaaatcca gggcgttccg
     5041 catgaattta ctgctatcga tggtgtagta gaagatgtaa cccgaatcat attaaatttg
     5101 aaaaaactac gatttaaata cgaaagcgaa ctgccaaagg ttctgcacat agtcaaggac
     5161 ggcgaagcag aactgacagg agcagatttt gcaatagata gtgatgtgga aataatgaat
     5221 cctgaaactc atattgcaac tattgagcgt ggtggcagga ttgatatgga agtgcagatt
     5281 gagcgtggca gaggctattt gcctggtgaa ataagcaagt cgagtgtaga aactattgga
     5341 gttattccta ttgatgcaat tttttcacca atacgaaaag ttaatataaa ggttgaagat
     5401 acacgagtag gtcaacgtac tgactatgat aaattgatta tagaaatatg gacagatggt
     5461 tcggttaagc ctgaagatgc actggcgcag gcagcaaaaa tcataaaaga tcatatgacc
     5521 atatttatta attttgaaga agaagttgag gaagaggaag tggctgtaga tgaaagccac
     5581 gataagctga aagtaatcct ggcaaaatca attgatgagt tggaattttc ggtgcgt
//
LOCUS       DAOSZP010000060         5635 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_12056_length_5635_cov_3.279749, whole genome shotgun sequence.
ACCESSION   DAOSZP010000060 DAOSZP010000000
VERSION     DAOSZP010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5635
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12056_length_5635_cov_3.279749"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..983)
                     /locus_tag="PK348_02210"
     CDS             complement(<1..983)
                     /locus_tag="PK348_02210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65057.1"
                     /translation="MKFVHITVFIISFSILATGFAGTIPFEQSSAKGYFERSLQSAMA
                     EKTKEAFIHKAMQAADTAYSIWEKEALTAYDGAIVTGEDDISGFLKTEARTAIQKMLE
                     EALGSYDARCFEIVVPEYAKEELYARINQMMQEDPHEDMVATYSAWDENVGHYVQQLL
                     AGIDSQLQTTRDALLSEINTTTQEYRKGFIETLDEKLNNIHKLIQRDIELYYVGAKNT
                     YFYQHYSDTASLRFYTQQQSASQITAQILESTTDKTDAILTEVQNKLSYTISSIGLPP
                     EGDDLDMLVQAGIYAWKAAEDDLLSARLSWERSSLDGYRQADEIWAKSFEQ"
     gene            complement(980..1666)
                     /locus_tag="PK348_02215"
     CDS             complement(980..1666)
                     /locus_tag="PK348_02215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65058.1"
                     /translation="MAGTIKIVISSFALAILCCGFATPVHVSAATHIPSTSRLRIIEA
                     TQKASQYMAAMKAMQCIEEEAMLAMHSYTIKAGGYSFSLHNDGISPAATHIPPAWNVL
                     THSFYSHTNVMLCMVYVMLLAVAYCYERFYYRQREAYPPCEFLYHELSHIYINACGIY
                     CIPSIIFKTPVNSGHTINKSFLPMYMSLLPCSVLYCMAVSRFQHIHTKTSPLEKSVHI
                     SISVKHEVRL"
     gene            1984..2322
                     /locus_tag="PK348_02220"
     CDS             1984..2322
                     /locus_tag="PK348_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HXK65059.1"
                     /translation="MDITKRTKDDVVILDISGEIDLYNAPEIKDIINSLIEEKKFNVI
                     INLEKVTYIDSSGIGALISSLSNLKKYQGGLKIINVFASVRKVFELTKLTSFFEIYDS
                     EEAAIESFKR"
     gene            2714..4033
                     /locus_tag="PK348_02225"
     CDS             2714..4033
                     /locus_tag="PK348_02225"
                     /inference="COORDINATES: protein motif:HMM:NF025704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4398 domain-containing protein"
                     /protein_id="HXK65060.1"
                     /translation="MHINKNVAVLAAIVCLSMITACFEKVPVKELSLAKNAITEAESV
                     KADYYAKDEYTAAKDLLLKAHDNVKNEEYDKAKQNAQESQKKAKEAYDKALPLLAKDT
                     LDIATQSLKAADDAYASVLAKEDYTKAADMLQEANKQYENKNYLAAYKAALDADLYAK
                     NARNVAMNQKETLADSIKEVNIVIAQAESYGAAQYAPEKLALAKEHSQIARSAYEAGE
                     LKKGFSAIEVAKLNADDAYFVSLKATAAKKIEEAQAAIDAAKGSEAAKLSPNDIKAAE
                     ESLAQAKSLFNDGKYKESMIASGQAINIAQGVSSKKAVAVAPIVEQQGKEGVAVKEEQ
                     KEAIQKKVEEETEYTLYKVKYNPAKRDCLWRIAEKFYNDGFKWKVIYDANRDIVKNPD
                     LIKPGWILKIPKAATKLTQPVNPVKEKNTDAIKENEPPATELKMKTE"
     gene            complement(4071..4847)
                     /gene="mazG"
                     /locus_tag="PK348_02230"
     CDS             complement(4071..4847)
                     /gene="mazG"
                     /locus_tag="PK348_02230"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HXK65061.1"
                     /translation="MSIPPLYKLKEIASILRSEHGCAWDKAQTSDTLKPYLIEEAYEV
                     YQAIENGNTENLKEELGDLLYQIYAHAQLASEQNIFDIDDVASGIIEKLIRRHPHVFG
                     NETAKDKYEVIDRWEKIKKKEKEDGQSMLNGVPVHLPALLKAYRVQQKVSRVGFDWEH
                     IQDIVVKLHEEVNEFKDALQNNKDSREALKEEIGDILFTIVNIARHMEINPEEALQHT
                     VSKFIARFQHIEKRLHEMNKDITQTSLQELDELWEESKGE"
     gene            complement(5155..>5635)
                     /locus_tag="PK348_02235"
     CDS             complement(5155..>5635)
                     /locus_tag="PK348_02235"
                     /inference="COORDINATES: protein motif:HMM:NF027666.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4976 domain-containing protein"
                     /protein_id="HXK65062.1"
                     /translation="DITPTILDLLNIQQPQSFDGTSLMKNHNRTFDAYCETGIWFDNT
                     GNFFFQKKRIMYPDITGISTIDFNHHDEITVTPYYDNLITIAKHRSIVANNLKLIYMP
                     THYGIEYELYDISQDPEERNNIYKTSPYANSMKQKLFLLFNNDRKIKIYNSYFMPVE"
BASE COUNT         1756 a          982 c         1133 g         1764 t
ORIGIN      
        1 aattgctcaa agctttttgc ccagatttca tcagcctgcc tgtacccatc taatgatgag
       61 cgttcccatg acagtcgtgc tgacagcaaa tcatcctcgg ctgccttcca tgcatatatt
      121 cccgcctgta ccagcatatc aaggtcatca ccttcaggtg gtaagccaat actgctaatg
      181 gtgtatgaca gcttattttg aacttctgtt aagatggcat ctgttttgtc tgtagttgat
      241 tcaagtattt gtgcagtaat ctgtgatgct gattgctgct gggtgtaaaa gcgtaaactt
      301 gccgtatcac tgtagtgctg gtaaaaatac gtattttttg caccgacgta atagagctca
      361 atatctcgct gtataagctt gtgtatattg tttagttttt catctaatgt ttcaataaat
      421 cctttacggt attcctgcgt ggtggtattt atctcagaca gcagtgcgtc gcgtgtggtt
      481 tgtaactgtg agtcaattcc tgctaaaagc tgttgcacat agtgcccaac attttcatcc
      541 catgcactgt atgttgccac catgtcttca tgggggtctt cctgcatcat ctggtttatg
      601 cgtgcataca gctcttcttt tgcatactca ggaacaacta tctcaaaaca gcgtgcgtca
      661 tagctgccca gagcttcttc aagcatcttc tgtatggcag tgcgggcttc tgtcttgaga
      721 aaacctgata tgtcatcctc tccggtaact atcgccccgt catatgctgt taatgcttct
      781 ttttcccata tgctgtatgc ggtatctgca gcctgcatgg ctttatgtat gaaagcttct
      841 tttgtcttct cagccattgc agattgaaga gagcgttcaa aatatccttt tgcactgctc
      901 tgttcaaatg gaattgtgcc ggcaaagcca gttgccagaa tgctgaaaga aataatgaaa
      961 acagtaatat gtacaaactt catagacgca cctcgtgttt aactgaaatt gatatatgta
     1021 cggatttttc caatggtgat gtttttgtgt gtatatgctg aaaacgtgac acggccatgc
     1081 agtaaagaac gctgcaaggt aacagtgaca tgtacatggg aagaaaactt ttgttgatgg
     1141 tatgccccga attaacaggt gttttaaaaa ttatggaagg aatacagtat atgccacaag
     1201 cgtttatata tatgtgcgat agttcatggt aaaggaactc acacggggga taggcctcgc
     1261 gctggcggta ataaaaacgc tcgtagcagt aggcaacagc cagaagcata acgtatacca
     1321 tgcacagcat tacatttgtg tgtgaataga aactatgtgt aagcacattc catgcaggag
     1381 ggatgtgagt ggctgcaggc gatattccat cattatgaag tgaaaaagaa tatccgccag
     1441 cttttatggt atatgaatgc atggcaagca tggcctcttc ttcaatgcac tgcatagcct
     1501 tcattgctgc catgtactgg ctggcttttt gtgttgcttc aatgatacgc agcctgcttg
     1561 tagaaggtat atgggtagcg gcagatacat gcacaggtgt ggcaaatcca cagcacagta
     1621 ttgccagtgc aaaagaggat attacgattt ttattgttcc tgccatgctg ttataatgaa
     1681 aatataaaca agaaaaatgc tttaaataga atttctatat gtacattaag tatatatttg
     1741 tactgtaatg ttaataaaat tgaatataat aatattgatt atgtcaataa aaaaatctgg
     1801 tggtaaaaca atttttttat tttatgtagt atttcattct agaatttgca cagtattaga
     1861 acaggttgta aaaaaaaaat tgtttacaaa acaataaaaa taaaatatct tgacatttta
     1921 tttcagtatt gtagtattat ctgatgatgc actgttagca ttattttggg gagttaacta
     1981 tttatggata ttacaaaaag aactaaggat gatgttgtca tactggatat atctggtgag
     2041 atagaccttt acaatgctcc ggaaattaag gacatcatca acagtcttat agaagaaaaa
     2101 aaatttaatg tcatcattaa tctggaaaaa gttacgtata ttgactcttc agggattggg
     2161 gcattgatat ccagcctttc aaatttaaag aaatatcagg gtggattaaa gatcattaat
     2221 gtttttgcat cagtgcgaaa ggtatttgaa ctcacaaagt taacgtcctt ctttgaaata
     2281 tatgattctg aagaggctgc catagagtca tttaaacggt aagatgctag cagattgtgt
     2341 gtaattactt ctataatata ctgaagagtg gttcataaaa aatagaaatt tttgacacat
     2401 ttgattttga aattaatgca ggtaaatagt tttcataatg taatgttttt gggcgatagt
     2461 aaccgcaagg aatgtttatt ttaatatcag gatttctctt cctgaaattt aagtatacag
     2521 attgctactg atagtagcat catgaatgta attttgagtc gtacagtata atggagatac
     2581 ccgatcaagt ttacataaca gtgggaaatt ttttaagtca agcttttaca tgatagaaca
     2641 taaactcata atggcagcat gaaggccata gtgatcaaat gaataaacca taaacataat
     2701 ttcggaggaa tatatgcaca taaacaagaa cgtcgctgta ctggcagcca ttgtatgttt
     2761 gtcaatgatt actgcctgtt ttgaaaaggt tcctgtaaag gaactatcgc ttgcaaaaaa
     2821 tgcaatcact gaagctgaat ctgtaaaagc ggattactat gcaaaagatg aatatactgc
     2881 agctaaagat ttgcttttaa aagcccatga taatgtaaag aatgaagagt atgataaggc
     2941 aaaacagaat gctcaagaat cacagaaaaa ggcaaaggaa gcctatgata aggctttgcc
     3001 attacttgca aaagatactc tggatattgc aactcagagc ctgaaggctg ctgatgatgc
     3061 atatgcatca gtacttgcaa aggaagatta tacaaaagca gctgatatgc tgcaggaagc
     3121 aaataagcag tatgaaaata aaaattacct tgctgcatat aaagctgcgt tagatgcaga
     3181 tttgtatgct aaaaacgccc gcaatgtagc aatgaaccag aaggaaactc tggctgattc
     3241 cataaaagaa gtgaatatag tgatagcaca ggcggaaagt tatggcgcag cacagtacgc
     3301 tcctgagaaa ctggctcttg caaaagagca ctctcagatt gcccgctctg cgtatgaagc
     3361 tggtgaactt aaaaaaggtt tttcagcaat agaagttgcc aagctcaatg ctgatgatgc
     3421 atacttcgta tcgctgaaag caactgctgc caagaagatt gaggaagctc aggcagcaat
     3481 agatgcagca aaaggcagtg aggcagctaa actatccccc aatgatataa aggctgcgga
     3541 agaatcgctg gcacaggcaa aatcattatt taatgatggc aagtataagg aatcaatgat
     3601 tgcctcaggg caggctataa atattgcaca gggagtgtcc tctaaaaaag cagtagctgt
     3661 tgccccgatt gttgagcagc aggggaaaga gggtgtagcg gtaaaagagg aacagaaaga
     3721 agctatacag aaaaaggtgg aagaagaaac tgaatataca ctgtataaag ttaagtataa
     3781 tcctgcaaaa agagattgcc tgtggcggat tgctgaaaag ttttacaatg atggattcaa
     3841 atggaaagta atctatgatg ctaaccgtga tatagtaaaa aatcctgatt taataaagcc
     3901 gggctggata ttgaagatac caaaagctgc aacaaagctc actcagcctg taaatcccgt
     3961 aaaagagaaa aatactgatg caattaaaga gaatgaaccg ccagcaaccg aacttaagat
     4021 gaagaccgaa taatgtacat acaaggggct gcgctagcag ccccttgttg ttattcccct
     4081 tttgattctt cccatagttc atccagctcc tgcaatgatg tttgtgttat atccttattc
     4141 atttcatgca gtcttttttc tatgtgctga aagcgcgcaa taaactttga gacggtatgt
     4201 tgcagggctt cttcgggatt tatttccata tgacgcgcaa tattgacaat ggtaaatagt
     4261 atatcaccaa tttcttcttt gagtgcttcc cggctgtctt tgttattttg caatgcatct
     4321 ttgaattcat ttacttcttc atgtaacttg acaactatat cctgaatatg ttcccagtca
     4381 aaaccaacac gtgatacctt ttgctgcaca cggtaagctt tcagaagtgc cggcagatgt
     4441 accggtactc cattcaacat agactggcca tcttcctttt ctttcttttt tatcttttcc
     4501 cagcgatcaa ttacctcgta tttatctttt gcggtttcat tgccaaatac atgggggtga
     4561 cggcggatta atttttcaat aataccactg gcaacatcat caatatcaaa tatattctgt
     4621 tcacttgcaa gctgagcgtg tgcatagatc tgatataaca ggtcgcctaa ctcttcttta
     4681 agattttcgg tgtttccgtt ttctattgcc tgatacactt catatgcctc ttctataaga
     4741 taaggtttga gtgtatctga cgtttgtgct ttatcccacg cacatccgtg ttcactgcgc
     4801 aatatactgg caatttcttt taatttatac aggggtggta ttgacatggg ttttctccag
     4861 ataatgaatg tgcattatgt aatgatgtat tgagtgtata gattactaaa gagcaaatgg
     4921 tatatagtat ataataattc aatggtttca attaacgtaa aagtaatcca aaaaaaatta
     4981 catactacga ttatatcgcg tagtatacat ttcatcaatc atctttgacg tgaattgata
     5041 atactcgctc atttcgatac aattttgcca taaaatctgg caaaattact caatgagcgt
     5101 tgcaaaattt gtatcgaaac gccatttggg acactcccgt gttggttggc cttatcattc
     5161 aacaggcatg aaataactat tatatatttt tatcttccta tcattgttaa ataataaaaa
     5221 aagtttttgt ttcatactat ttgcatatgg tgatgtttta tatatattgt ttcgctcctc
     5281 agggtcctgt gatatatcat acaattcata ttctatccca taatgcgtag gcatgtagat
     5341 aagttttaga ttgtttgcaa cgatgctacg gtgttttgct atggtaatca gattgtcata
     5401 atatggtgtt actgtgatct catcatggtg attaaaatct atggttgata tgccagttat
     5461 atcaggatac attattcgtt ttttctgaaa gaaaaaattt ccagtattgt caaaccatat
     5521 accagtttca caatatgcat caaatgtacg gttatggttt ttcataaggg atgttccatc
     5581 aaaggattgc ggttgttgta tatttaaaag atcaagaatt gtaggtgtaa tatct
//
LOCUS       DAOSZP010000061         5625 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_12085_length_5625_cov_3.215081, whole genome shotgun sequence.
ACCESSION   DAOSZP010000061 DAOSZP010000000
VERSION     DAOSZP010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5625)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5625)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5625
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12085_length_5625_cov_3.215081"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="PK348_02240"
     CDS             <1..202
                     /locus_tag="PK348_02240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65063.1"
                     /translation="DYVKSQITIKKGDNVTILGSKTTMNNETVIITKTVKTKDKEVTF
                     RNDDGTPKWAGQMKYKKYKMNQ"
     gene            complement(203..2812)
                     /locus_tag="PK348_02245"
     CDS             complement(203..2812)
                     /locus_tag="PK348_02245"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HXK65064.1"
                     /translation="MPEIKFDTIRKNIESNPLYGAAIGLILGLLIIGFLFTNVYRLFE
                     YKLFDVRFKIKPQLPQWEYLTFLDIDDNSISNVGQFPWPRYIYANGLEVIKDIAPKQI
                     AFDIQFMDKSPRYAQPDILKIVEEKVKTGKRITYEELNQAILDSDRLFSEAIAKNNVI
                     LAYSFLNRTLAETKLTPEEQQKRNESIQRFEKIASIPVPKDKQKEFSRYVDPTRVIIQ
                     YPIPELAMKARDFGYVDSDFDIDGIARRIRLIRVFNDRIYFHMAMVMLMDLMKVKKTD
                     VEIVHGKHIILHNAIDPITLQKKDYVIPIDDAGMSYIHWSGEFTETFNHVSFYALLEY
                     NQLKDTIHEFFDEQEVQSKNTERSRLYGELATMHEIFEKTKDMKAKQDLWEKIKTNRK
                     RLYEIESGYIKNLENQLAEINKQLKETNDNQLKEASKNLINFINAARLVLEVDRLKDH
                     SCIIGLTATASHDIGVTPLSSEYLMVGTYHNFINTVLQQAYIYQVPQWIDYCLILLLA
                     IFLVLAVQRLSAMGTITAIVGTLVVYNIINIALFAFQNLYMDQLGVSLAILLPSAIVS
                     SVKFMSEESQKRYIKEVFSKYMAPRVVEELIKDPGKLQLGGELRNISIFFSDVAGFSS
                     ISEKLTPQELVALLNEYLSEMTDIILKYEGTVDKYEGDAIIAFFGAPLAFEDHPERVC
                     LAAIDMKRRLAELREEWRKKGTFELKVRMGINTGEAVVGNMGSRQRMDYTMMGDAVNL
                     ASRLEGANKYYGTYTMISETTYERVKDVVEARELDLIRVVGKEKPIKVYELFGKKGTL
                     PDYMYEMLKKYNEGLQLFRERQWEEAQSAFKAGLRIVPDDGPCKTYIERCKEFAKNPP
                     PKKWDGVYRLKTK"
     gene            complement(3024..3635)
                     /locus_tag="PK348_02250"
     CDS             complement(3024..3635)
                     /locus_tag="PK348_02250"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HXK65065.1"
                     /translation="MYISKNYYSLFPQLQKIIQLYEEVEKITKNVKNLFSVSCPHGCG
                     QCCKTASHNIEASIIEMLPLCVYLYEHTNYQLWLDKASDNLCPFYKAETLYDDAGCCA
                     VYEYRPLVCRLFGFSFMKNKYGAIVPVACSTLQNQYTAKKEIAAIHFSNLPHMSSLSM
                     QSMMIDPSRGERYPINEAFIKAMEYVILKIRLMHYQSEYNKTA"
     gene            complement(3725..4342)
                     /locus_tag="PK348_02255"
     CDS             complement(3725..4342)
                     /locus_tag="PK348_02255"
                     /inference="COORDINATES: protein motif:HMM:NF014707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HXK65066.1"
                     /translation="MKVSQLQTVLLHTCCGPCLTHVHSVLVSQYNIHIFYYNPNISPL
                     SEYKRRLYEVEQFSHLHSIPVTIGHYNQREWFNAVKPYRFYEELSPRCHACYAFRLEE
                     TFKRAREQNFDAVCTTLTVSPYKDAEKINEIGKTLSKVYNITYIPSDFKLDGGYQHSI
                     ELSKQYEMYRQKYCGCIYSKLERKKYSLWSKRVQAFKESFDALNK"
     gene            complement(4339..4695)
                     /locus_tag="PK348_02260"
     CDS             complement(4339..4695)
                     /locus_tag="PK348_02260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65067.1"
                     /translation="MKKRNKASFGVNGWYLSPSDNICLAKCIFDEPYREEIKEINKHE
                     IIVKIADCIGPEPSNLCIAEEIFEKLKFEIANFVIHHEYNCTRGVNAGLLLWLDGKVN
                     PAKLKKLNSFIDKYLK"
     gene            5153..>5625
                     /locus_tag="PK348_02265"
     CDS             5153..>5625
                     /locus_tag="PK348_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014509903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HXK65068.1"
                     /translation="MATCNANPVLLSCNITNVATPSSPLYSKTYRDASVIHLPYNISI
                     SENTLAIIPGPCSIESEEQIMATAEFVKKSGAAILRGGAFKPRTSPYAFQGMGIAGLQ
                     LLRKAGDYYRMPIITEVLDSEDITIVADYADILQVGARNCQNFSLLRKLGKIRKPV"
BASE COUNT         1745 a         1049 c          874 g         1957 t
ORIGIN      
        1 tgactatgtt aaatcacaga taactattaa aaaaggtgac aatgtaacaa tcctgggttc
       61 aaaaacaaca atgaataatg aaactgttat cattacaaaa actgtcaaaa ccaaagataa
      121 agaagtaact ttccgtaatg atgacggtac accaaaatgg gccggtcaga tgaagtataa
      181 aaagtataaa atgaatcaat aattattttg tttttaacct gtatacacca tcccattttt
      241 tcggtggtgg atttttagca aattccttgc aacgttctat atacgtttta caggggccat
      301 catcaggaac aatgcgcaat cccgctttaa aagcagattg cgcttcttcc cattgtcttt
      361 ctctgaatag ctgtaatcct tcattatatt tcttcaacat ttcatacata taatctggaa
      421 gcgtgccttt ttttccaaat aattcatata ccttaattgg cttctccttt ccaacaaccc
      481 gtattaaatc taattccctt gcttcaacaa catcttttac ccgttcatag gttgtttcac
      541 tgatcatagt atatgtacca taatacttgt tagctccctc caatcgcgaa gccaggttaa
      601 cggcatcacc catcatggta taatccatac gctgccggga tcccatatta ccaacaacag
      661 cctcacctgt attaattccc attcttactt tcagctcaaa tgtgcctttt ttacgccatt
      721 cttccctgag ttcagccaac cgtcttttca tatcaatagc tgccaggcac accctttcag
      781 gatgatcctc gaatgcaagt ggtgcaccaa aaaaggctat tattgcatcc ccttcatatt
      841 tatctaccgt accttcatat tttaagatta tatctgtcat ttctgataag tattcattga
      901 gtaatgcaac aagttcctgg ggtgttaatt tttcagaaat ggatgaaaat ccagccacat
      961 ctgagaaaaa gatacttata tttcgcagtt ctcctcctaa ctgtaattta cctggatcct
     1021 ttatcagttc ttcaactaca cgtggtgcca tatatttaga aaacacttct ttaatatacc
     1081 gtttctggct ttcctcactc ataaacttga cactacttac tattgctgat ggcaacagta
     1141 tagccagcga cacacccagt tgatccatat aaagattttg aaatgcgaat aatgctatgt
     1201 ttataatatt gtaaacaaca agcgttccca caattgctgt aattgtaccc attgcactta
     1261 accgttgaac agcaagtacc aaaaatattg ccagcaataa aattaaacaa taatctatcc
     1321 attgaggaac ctgataaatg taggcttgct gtaaaaccgt gttaataaaa ttatggtatg
     1381 taccaaccat tagatattct gatgataggg gtgtcacccc aatatcatgt gaagctgttg
     1441 ctgttaatcc tattatgcat gaatgatctt ttaatctgtc aacctcaagc accaggcgtg
     1501 cagcgttaat aaaattaatt aaatttttac tggcctcttt caattgatta tcgttggttt
     1561 cttttaattg cttatttatt tcagctaact gattttccag attctttata tatccacttt
     1621 ctatctcata caaccgttta cggttagttt ttattttttc ccatagatcc tgtttggctt
     1681 tcatgtcttt tgttttttca aatatttcat gcatggtagc cagttcacca tataacctgc
     1741 ttcgttcggt gtttttggac tgcacttcct gttcatcaaa gaattcatgt attgtgtctt
     1801 ttaattgatt atattcaagc agtgcataaa atgatacatg attaaaggtt tcagtaaatt
     1861 cgccactcca gtgaatatag gacataccag catcatcaat aggaatgacg taatcctttt
     1921 tttgcaatgt tattgggtca attgcattgt gcagtatgat atgttttcca tgaactatct
     1981 ccacatcagt ttttttaact ttcattaaat ccatcagcat aaccatagcc atatggaaat
     2041 atattctatc attgaataca cgtataagtc gtatgcggcg ggcaatacca tcaatatcaa
     2101 agtcactatc cacatatcca aaatccctgg ccttcatggc aagttctgga attggatatt
     2161 gaataattac tctggttggg tcaacataac gagaaaattc cttttgctta tctttgggaa
     2221 ccggaattga agctattttc tcaaatctct ggatggattc gttccgtttt tgctgctctt
     2281 cgggtgttaa ttttgtttct gcaagagtgc gatttaaaaa cgaatacgcc agaataacat
     2341 tatttttggc gatagcttct gaaaatagtc ggtcgctgtc cagtattgcc tgattaagtt
     2401 cttcgtaagt gattcgcttg ccagttttta ctttttcttc tacaattttc aatatatcag
     2461 gttgagcata ccgaggtgat ttatccataa attgaatatc aaatgctatc tgttttggcg
     2521 ctatatcctt tatgacttca agaccattag cgtagatata ccgcggccat ggaaattgac
     2581 ctacattgct gatgctgtta tcatcaatat caagaaaggt tagatattcc cattgtggaa
     2641 gctgtggttt tattttgaac cgcacatcaa ataatttata ctcaaaaagc ctgtatacat
     2701 tagtgaataa aaaaccaata ataagcaaac ccagaatcaa gcctatagca gccccatata
     2761 acggatttga ttcaatattt tttcttatag tatcaaactt tatttctggc ataaaccacc
     2821 tgcttatctt tatttttctg cattataaca gattatgttt aaatattaca agtataataa
     2881 tattttgtta tcttatttat tccatttctt ttggatacaa agatatcttt aattatcctg
     2941 ccacaaaaag tggtaaaggt aataaatttt ataaatttac aaaatagtat tttttgaaat
     3001 tatcagacaa atactaaatt attttatgca gttttattgt attctgattg atagtgcatc
     3061 aaacgtattt tcaagattac atattccata gcttttataa aagcctcatt aatgggatat
     3121 cgttcacctc tggaaggatc tatcatcatt gactgcatag aaagagaact catatgcggt
     3181 aaattactga aatggatggc agctatttct ttttttgcag tatactgatt ttgcagcgtg
     3241 ctacatgcta caggtactat cgccccatat ttatttttca taaaactaaa accaaataag
     3301 cgacacacca atggcctgta ttcatacaca gcacaacacc ctgcatcatc gtataatgtt
     3361 tcagctttat agaaagggca caggttgtct gaagccttat caagccataa ctggtaatta
     3421 gtgtgttcat acaaatatac acataatggt aacatttcaa ttattgacgc ttcaatattg
     3481 tggctggctg ttttgcagca ctgaccacag ccgtggggac agctgacaga aaacaaattt
     3541 tttacatttt tggtgatttt ttcaacttcc tcatacaact ggataatttt ttgtagttgt
     3601 ggaaataacg aataataatt tttactaata tacataatca cacaacacca ttatcgtaaa
     3661 ttagaaaatt attattgtta catcaggtag aaaattcaaa atatgaaacg tactgtcaac
     3721 ttttttattt atttaatgca tcaaaagatt ctttaaatgc ttgtactcgt ttagaccata
     3781 aagaatattt ttttctttca agctttgagt atatgcatcc acaatatttc tggcgataca
     3841 tctcgtattg ttttgataat tctattgaat gctgataacc accatctaat ttaaaatcag
     3901 aaggtatata tgttatgtta tatacttttg acagcgtttt gcctatctca tttatttttt
     3961 cagcatcttt atatgggctg acagttaacg tggtgcatac agcatcaaaa ttttgttcgc
     4021 gcgcacgttt aaatgtttcc tctaaacgga atgcatagca tgcatgacag cgcggcgata
     4081 gctcctcata aaagcggtaa ggctttacgg cattaaacca ttctctttga ttatagtgtc
     4141 cgatagttac cggaattgaa tgaagatgtg aaaattgttc cacttcatac aatcgccttt
     4201 tatattctga cagtggagat atattgggat tataataaaa aatgtggata ttatattgag
     4261 acacaagaac agagtgtaca tgtgttaaac acggtccaca acatgtgtgt aaaagtacag
     4321 tttgtaattg actaactttc acttaagata tttatcgata aaactattta actttttcaa
     4381 ctttgcagga tttactttgc catcaagcca caataaaagg cctgcattca caccacgggt
     4441 acaattgtat tcgtgatgaa tcacaaaatt tgcaatttca aatttcagct tttcaaaaat
     4501 ctcttcagct atacacagat ttgaaggctc aggtccaata cagtcagcaa tttttacgat
     4561 gatttcatgt ttgttgattt ctttgatttc ctcacggtaa ggttcgtcaa aaatacattt
     4621 agctaaacaa atattgtctg aaggagataa ataccatcca tttaccccaa aagaagcctt
     4681 gtttcttttc ttcattgctt attcctcttg taccatggca agatataaaa aatatgtaaa
     4741 agcaatataa tatttctatt aaatatataa aaataataac aaaatgtcaa atatttaaaa
     4801 ctaaattgtt tcatgaaatg aatttttttt tatattttat atacatttgc catttgtgat
     4861 taaaattatt ttatttttta cttgacatga ttacattaaa ttggttattg atttatacat
     4921 ctttgaatta atgtactatt tatgaaacta tcattgtata acatcatcag caaaagctat
     4981 tatttctggt tttattattt ttatagcagg gctcgtctgt agcttttgct gatgctgtta
     5041 ttatgcaagg gccgcaggcg ggtagccggc ggcctttgtt atttatggaa taatgtataa
     5101 ggccgccacc ctccgggctc ttaattgact ttaaaatttt tcggaggaat atatggccac
     5161 atgtaatgca aatcctgttt tattatcctg caatattact aatgttgcaa caccatcatc
     5221 accgctttat agtaaaactt atcgtgatgc ttcggtaatt cacctgccct ataatatatc
     5281 aatttctgaa aacacattgg caatcatacc aggcccatgt tcaattgaat ctgaagaaca
     5341 gattatggca accgctgaat ttgtaaagaa atcaggtgca gctatattac gtggcggtgc
     5401 tttcaaacca aggacatctc catatgcatt ccagggtatg ggtattgcag gattacagtt
     5461 gttacgaaaa gcaggtgact attacagaat gcctattatt acagaagttc ttgatagtga
     5521 agatattact attgtagctg actatgctga tatcctccag gttggcgccc gcaattgcca
     5581 gaatttttcc ctgctgagaa aattgggaaa aattcgtaaa ccagt
//
LOCUS       DAOSZP010000062         5624 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_12089_length_5624_cov_2.952236, whole genome shotgun sequence.
ACCESSION   DAOSZP010000062 DAOSZP010000000
VERSION     DAOSZP010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5624)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5624)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5624
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12089_length_5624_cov_2.952236"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..609)
                     /locus_tag="PK348_02270"
     CDS             complement(<1..609)
                     /locus_tag="PK348_02270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65069.1"
                     /translation="MKRILMVLFYMILSVSVAMFSLQCAGKAVKQAEPVSTDEVPRDT
                     ALKEDDSDTTYSKGLKLEKEESFATKESAKTKALAPKKMDGAKTASGLKAGFADDNKQ
                     FNYFVNFIKDYKDKVQHYPINISERIIFYCKDIAGKSIPNAKVNVFSGKRLLETGKTY
                     ADGSFMFYPSEYDTKYTTYVLTFEALNTKAQITVDRQGKREHT"
     gene            complement(743..1897)
                     /locus_tag="PK348_02275"
     CDS             complement(743..1897)
                     /locus_tag="PK348_02275"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HXK65070.1"
                     /translation="MPKEGFKTAKQKNDCIEAIIQELVNRNGFLIVGHTNPDEDCVAS
                     MIAFSLLAKKFDKQVKIFSSKKIQENFKYLVDIAMYNRIDVVTAIPRSFAVDTIVICD
                     TAKPSMIDANRTILQMLKKSTIRKIEIDHHIGADSDYAGDPGYRLVTEASSASELVGQ
                     IALKLRAKKEILKKYVILDPLSRNLVLAILTGIIGDSQMGRYLKSSREKRMYRIFSTL
                     FNDILSTSTIKESNITNMNQIFEQLKQASEKEKHCFQFIKKKSKVYKSVGYAMLTEDD
                     MRYLYENFDDDDIISTTRTLADMLAEDSGKVGLVSYYDIPEKSNLVQFRMRRSQNYKN
                     YDLREFLTTMGIENGGGHEGAIGFRLPKHAVDDYPKFVTSLVKKINALLP"
     gene            2394..3107
                     /locus_tag="PK348_02280"
     CDS             2394..3107
                     /locus_tag="PK348_02280"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXK65071.1"
                     /translation="MKKIALSCLITLLSVTIAYTISFAAKKDDDTPFKKGVKYFYQQK
                     FEIAELLFQEELKNNPENAMAYSYLGDIFLYKKRYDGALQLYIKALELNPKSAEDAFR
                     IGQVYYYKKQPEEALAYFKKAFSLNPQLKFAYYHIGLTYLMLLRDKEKTIESWEKFLA
                     IAPEDPQYEKIKRVIELLKDPKFVLPPPGSDVSIEEALHLGGIILDDVQREAQDKKAG
                     HETKKTKNKLEEIYRDDEL"
     gene            3136..5520
                     /locus_tag="PK348_02285"
     CDS             3136..5520
                     /locus_tag="PK348_02285"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXK65072.1"
                     /translation="MAYYYDRYRNLKIAGVVAVILLLLFGGYYFLSRPKDALKDIVDI
                     RLKQIELYEKILETNPNDVDTLIKLGNLYKETGNLDKAIDYYKKALALQPDNYNALNE
                     LGHAYALKGDYENAIKTLELAKKYFPKYPQAYNKLGNVYETQEKLDKALKEYKSAIAV
                     APQYQESYWNIAQQRMKKGDYKGAEEILKKGIKANPSDPEGYYNLGNLYMHKKQYAKA
                     IPQYQKAITLSPGTGKYYRALGNAYLAQNKIPEAVGAYSTALSKDPGDALSAERLGDI
                     YASQGNWAKAADYYKQALSYNSNDENLKNKYAHALSKLQKPSIIGGPDTQQLAVATKE
                     TTEPSAQQVISRDKDSKDLSEPTPQTTPANPVNDSALKWRELGDKYRAEKKYDEALKA
                     YAKATQLDKTDDHSHYWMGRIYYHNQMDDEAKKSFQNAIIANPKNADSHYHLGLIYYS
                     EGKYSPAVNEFTKAIEEKPVFAKAYYARGLAQYKMNNLQAAIKDLKQSIEQDPNLDRA
                     YFNLGDIYLKQKNYKVALDYFTKDRELRPNNPDTLFKIAETYHEMKSYSNAETFYNKT
                     LEADPEYFQALFNLGLIAATKQNYTQAISFYTKALSIKNDEPPTLYELGKAYEGLGDD
                     NKAIEYYSLAITKNPNYTKAYINLGNLYKKNKLHDKAIEQFKQATINDPKSFEAHYNL
                     ANAYLSANMNDEAIEEYKTAIDLSPMNDQAHFFLGIAYKEKGVFEQAVKEFERSLQIN
                     SSLSDAHEELGMIYYRKLKNNEKAIYHFEKLLSMKPNHPKADQIRDIITMLKKQ"
BASE COUNT         1888 a         1054 c          965 g         1717 t
ORIGIN      
        1 cgtatgttca cgcttcccct gtcggtctac cgtaatttgt gcctttgtgt ttaacgcttc
       61 aaatgtaagc acgtaggtag tatatttagt atcatattct gaagggtaaa acatgaacga
      121 tccatcagca tacgttttac ctgtttcaag aagtctttta ccactaaaaa cattaacctt
      181 agcattgggt attgatttgc ctgctatatc tttacagtag aaaataatcc tttcagatat
      241 attgatgggg taatgctgga ctttatcttt gtaatcctta ataaaattta caaaataatt
      301 gaactgttta ttatcgtcag caaaaccagc ttttaatcct gaagctgttt ttgctccatc
      361 catttttttt ggtgcaagcg cctttgtttt tgctgattct tttgttgcaa atgattcttc
      421 tttttcaagc tttaaccctt ttgaatatgt tgtatcacta tcatcttctt ttaatgcagt
      481 gtcacgtgga acctcatcag tgcttaccgg ctctgcctgt ttaactgctt tacctgcaca
      541 ctgcaatgaa aacattgcca ctgaaactga taaaatcata tagaataata ccataagaat
      601 tcttttcata ctattcctcc ggaaaatatg tgatgtggct gaaaaatcat atcaatcacg
      661 atgaactata gaaatttacc tttattctgt attgttctgt catgaaaaat atttagaaac
      721 ccacaaagta aacctgttgt cttcatggca aaagggcatt tatttttttt actaatgacg
      781 ttacaaattt tggataatca tccacagcat gttttggcaa cctgaagcca attgctcctt
      841 catgccctcc accgttttca atgcccattg tagtaaggaa ttcacgtaaa tcatagtttt
      901 tataattctg gctgcggcgc atacggaatt gcaccagatt tgatttttca ggaatatcat
      961 agtatgatac cagccccacc ttcccggaat cttcagcaag catgtcagcc aacgttcgtg
     1021 tagttgaaat aatatcatca tcatcaaaat tttcatagag atagcgcatg tcatcttctg
     1081 tcagcattgc atatccaact gatttataca ccttgctttt tttctttata aactggaaac
     1141 aatgtttttc tttttcagat gcctgtttca actgttcaaa gatctgattc atattggtga
     1201 tatttgattc ttttattgtg gatgtactta atatatcatt aaacagtgta ctgaatatcc
     1261 tgtacatccg cttttctcgt gatgatttca aataccgtcc catctgtgaa tcaccaataa
     1321 tgcctgttaa aattgcaagc accaggtttc gggacagtgg atccagaata acatattttt
     1381 ttaagatttc tttctttgcc ctcagtttca gcgcaatctg cccaacaagc tcacttgcac
     1441 ttgatgcctc ggttaccagc ctgtacccag gatcaccagc atagtcacta tcggccccaa
     1501 tatgatggtc tatttctatt tttctaatag tgcttttttt aagcatctgc agtatagttc
     1561 tgttggcatc aatcatggaa ggtttggcag tatcacaaat aacaattgtg tcaacggcaa
     1621 atgagcgggg tattgctgtg acaacgtcta tgcggttata catggcaata tcaacaaggt
     1681 acttgaagtt ttcctgtatt ttttttgacg aaaatatttt tacctgctta tcaaattttt
     1741 ttgcaagcag ggaaaatgct atcatagaag caacacaatc ttcatcagga ttagtatgtc
     1801 ctacaataag aaaaccattg cggttcacca attcctgaat tattgcttca atacagtcat
     1861 ttttttgttt tgctgtttta aatccttctt taggcatggg aatttccagt tacgttggca
     1921 gtaaatgtag aacaatatct ttaaaatgtc aacagtatat ttttttattt ttgtgttgct
     1981 tgacagggtg tattttattc attattttta aagagttgcc catggaaaca atgtggttat
     2041 taatgtttta tacgttgtca tttcgacgaa cattttcaca ctgtcatttt gacgaatgtt
     2101 tttatactgt catttcgacg aatgttttca tactgtcatt tcgacgaacg cagtgaggag
     2161 aaatcttatt ttacaacagc atcccgataa aatcgggatt cgaaatgaca tagagaataa
     2221 atattatttt caatggaggt ctacatgaat tttgagtgga aacggtttat agtagctttt
     2281 tgtacaatga tgctgttctt ttttgttaca gtttttacat gctgatgttc gtatactgtt
     2341 tttatgtatt ctgttatcgt catataaccg ataataatac tggaaatact tctatgaaga
     2401 aaatagcttt atcctgtctg ataacccttt tatcagtaac tatcgcatat accatatcct
     2461 ttgctgcaaa aaaggatgat gatacccctt ttaaaaaggg tgttaaatat ttctatcagc
     2521 aaaaatttga gatagccgaa ctcttatttc aggaagagtt gaaaaacaat cctgaaaatg
     2581 ccatggcgta ttcatacctg ggtgatattt ttttatataa aaaacgctat gacggtgcat
     2641 tgcagctgta tattaaagct ctggaattga atccaaaaag cgccgaagat gcattcagaa
     2701 taggacaggt atattattat aaaaagcagc cggaagaagc acttgcatat tttaaaaaag
     2761 cattcagcct taatccacag cttaaatttg cttactacca tattggcctg acgtatttaa
     2821 tgctgctcag ggataaagaa aaaaccattg agtcatggga aaaatttttg gcgatagctc
     2881 cagaagaccc acaatatgaa aaaataaaaa gggtcattga attattaaaa gacccaaaat
     2941 ttgtgttacc accaccagga agcgatgtat ccattgaaga agcattgcat ttaggtggaa
     3001 taatacttga cgatgtccag cgtgaagcac aggataaaaa ggccgggcac gaaacaaaga
     3061 agacaaagaa caagcttgaa gaaatttatc gtgacgatga attataagat aatttaacat
     3121 accagaggta ccattatggc atactattat gaccgttacc gcaatctgaa aattgcaggt
     3181 gttgttgcag taatactatt actgctattt ggcggatatt attttctttc tcgacccaag
     3241 gatgctctaa aggatatcgt tgatatacgg cttaagcaaa ttgaattata tgaaaagatt
     3301 ctggaaacaa atcccaatga tgttgatacc cttataaagt taggaaatct ttataaagaa
     3361 acaggtaatc tggacaaagc aattgactac tataaaaaag cactggcact gcaacctgat
     3421 aattacaatg cattaaacga attgggacat gcctatgccc tcaaaggcga ttatgaaaat
     3481 gccattaaaa cactggaact tgccaaaaag tacttcccta aatatcccca agcctataat
     3541 aaattgggaa atgtatatga aacacaggaa aaattagaca aagcccttaa agaatataaa
     3601 agcgctattg ccgttgctcc ccagtatcag gaaagctact ggaacatagc gcaacaacga
     3661 atgaaaaaag gtgactataa aggtgcagaa gaaatcctga aaaaaggcat taaagccaat
     3721 ccttctgatc ccgaaggata ctacaactta ggcaatctgt atatgcataa aaagcagtac
     3781 gctaaagcta tccctcaata ccagaaagca ataactcttt caccgggcac aggtaaatat
     3841 taccgtgcat taggcaatgc ataccttgcc cagaataaaa tacctgaagc tgttggtgca
     3901 tattctacag cgctgtcaaa agatcctggc gatgcattat ctgctgagag gttaggcgac
     3961 atctatgctt cacagggcaa ctgggctaaa gcagccgatt attacaaaca ggctttaagc
     4021 tataacagta atgatgaaaa tttaaaaaac aaatatgcac atgccctttc aaaattacaa
     4081 aaaccttcta ttattggtgg gcctgataca caacaacttg cagtagcaac aaaagaaaca
     4141 acagaaccct cagcacaaca ggtaattagc agagataaag attcaaaaga tttatcagaa
     4201 ccaacacctc aaacaacacc ggcaaatcct gtcaatgatt cggcactcaa atggcgtgaa
     4261 cttggagata aatatcgtgc agaaaaaaaa tacgatgaag cgctgaaggc atacgccaaa
     4321 gcaacacagc ttgataaaac cgacgaccat tcgcattact ggatgggacg catctattat
     4381 cataaccaga tggatgatga agcaaaaaaa tctttccaga atgcaattat tgctaacccc
     4441 aaaaatgctg attcccacta tcatttaggg cttatctatt attctgaagg gaaatacagc
     4501 cctgcagtta atgaatttac aaaagccata gaagaaaaac ccgtatttgc aaaggcatac
     4561 tatgcacgag gccttgcaca gtataaaatg aataatctgc aggcagctat taaagactta
     4621 aaacaatcta tagaacagga tccaaactta gatagggcat attttaattt aggtgatatc
     4681 tatcttaaac agaagaatta taaggtggca cttgattatt ttaccaaaga cagggaatta
     4741 aggcccaata atcctgacac attgttcaag atagccgaaa cctatcatga aatgaaatcg
     4801 tattcaaacg ctgaaacatt ctataacaaa acacttgaag ctgaccctga atacttccag
     4861 gcacttttca acttagggct tattgcagcc acaaagcaga attacacaca ggctatttca
     4921 ttctacacca aagcattatc aattaaaaat gatgaaccgc caacactgta tgaattgggt
     4981 aaggcatatg aggggcttgg tgatgataat aaggcaattg aatattattc attagctatc
     5041 actaaaaacc caaactatac taaagcttac atcaacttag gcaacctgta taaaaagaac
     5101 aaactgcatg acaaggccat agagcaattc aaacaggcta ctattaatga ccccaaatcc
     5161 tttgaggccc actataactt agcaaatgca tacttatctg ctaacatgaa tgatgaagct
     5221 attgaagaat ataaaactgc tattgatctt tcaccaatga atgatcaggc acatttcttt
     5281 ttaggtattg cctataagga aaagggcgtt tttgaacagg ctgtaaaaga atttgaacgt
     5341 tcactgcaga taaacagttc actatctgat gcacatgaag agcttggcat gatctattac
     5401 cggaagctta aaaacaatga aaaagctatc tatcattttg aaaagctttt atcaatgaag
     5461 ccaaatcacc caaaagctga ccagatacgc gatattatta caatgttgaa gaagcagtaa
     5521 caataccatt ttttatttga catataacga tttgtgctat ttttgtatat taaattgata
     5581 attctacatt aacggttaaa caatgcgtaa atgtttattt tcaa
//
LOCUS       DAOSZP010000063         5586 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_12193_length_5586_cov_2.990056, whole genome shotgun sequence.
ACCESSION   DAOSZP010000063 DAOSZP010000000
VERSION     DAOSZP010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5586
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12193_length_5586_cov_2.990056"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1126)
                     /locus_tag="PK348_02290"
     CDS             complement(<1..1126)
                     /locus_tag="PK348_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010249095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliI/YscN family ATPase"
                     /protein_id="HXK65073.1"
                     /translation="MIQILPHEKVDIFSKYKDVLEEVDTLRATGRVEKITGLTIESHG
                     PDVQYGELCRIRLKSGNYLYAEVVGFNKNKIILMPIGDMKGVVPGADVYAVGSQLFVP
                     VGEALIGRVIDGLGNPIDGKSKLFLKERYPVDNKPLTPLEREIIDSPLPTGIRAIDGL
                     ITVGKGQRIGIFSGSGVGKSTLISMIARNTAADISVIALIGERGREAKDFVEKELQNE
                     GLKRSVVIISTSDQPPMVRLRAGFLAHSVAEYFRDKGLHVNLLMDSITRFAMAQREVG
                     LAAGQPAATRGYPPSVFSLLPQLLERAGNKKGAGSITGFYSILVEADDMNEPIADAVR
                     GILDGHVVLDRKLAHKNHYPAIDVLGSISRCMNDVIDEEHK"
     gene            complement(1148..2074)
                     /gene="fliH"
                     /locus_tag="PK348_02295"
     CDS             complement(1148..2074)
                     /gene="fliH"
                     /locus_tag="PK348_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008592071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HXK65074.1"
                     /translation="MSKLVFKPTEIQVISAPKQIELPEKYQKNVLTDEEYAEFEVDEE
                     GNPVEVYQGPSIEEMEAELERYRRETEEEVRRMLEDAKAKAAQIEEEGKKAAFLELQQ
                     AREQIKIEMERFKIESEREIERGKFEAEKMIKEAELKVSEIEHEAYKRGYDAGREEGY
                     KEGQAEVMRLIDRLGTIVSTAVDIRDDIIRSSEKLMTEMILMIARKVIKDEIVERREV
                     VINNIKEALQRVKDRDRIDIRVNFADLDMTTAHKDELIKMMESLKKVNIYEDSRVERG
                     GCIIETDVGAIDARISTQLESIEEAIRNARPI"
     gene            complement(2067..3083)
                     /gene="fliG"
                     /locus_tag="PK348_02300"
     CDS             complement(2067..3083)
                     /gene="fliG"
                     /locus_tag="PK348_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HXK65075.1"
                     /translation="MLQQKKSQLTGRQKAAIFLVSLGSDVSSEIFKHLREDEIEQLTF
                     EIARLDKVEPEDRDKVLMEFQELMMAQEFIATGGIDYAREVLERALGTQKAIDIVNRL
                     TSSLQVRPFDFIRRTDPSHLLNFIQGEHPQTIALILAYLDPQKAATILSGLSHQIQAD
                     VARRIATMDRTSPDVLREVERVLERKLSTLASEDYTSAGGIDAIVEVLTHVDRGTEKI
                     IIEALEEEDPELAEEIKKRMFVFEDIVLLDDRAIQKVLREVDTQDLAKALKGVDAEVQ
                     EKIFRNMSKRASALLREDMDFMGPIRLRDVEEAQQKIVNIIRKLEEAGDIVVARAGEE
                     ELVV"
     gene            complement(3232..4929)
                     /gene="fliF"
                     /locus_tag="PK348_02305"
     CDS             complement(3232..4929)
                     /gene="fliF"
                     /locus_tag="PK348_02305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HXK65076.1"
                     /translation="MEFFTKIFNQLKEIYSKLDTTKKIIIGVVTGVLIIAFIVLFSVS
                     SQKANVVLFSDLPSDEFGQVTKKLEEMGFHYTTSGTTTILVNPDQREIILTRLAQENM
                     IPKGIPGWKLFDISKWTETDKELNVKYMRALRDEIKRHIESLKNVDKADVEIAITEDE
                     LFADKENAYTAAVTVYLAPGYEGLTKKEIKGIQYLVARAVGTKLKPENVTVTDEYGKI
                     ISDFDDQFDKAKEEYTLLEYRKKIEEKARVQMLKDIRAGLEKIFSPDRIQIVRLNMDF
                     NWDKIQEEQEEYTPIEMEKDNPLTPYSERKVKDSLTISKKSTKEHFQGHGWNPEGPAG
                     TEGNKPPGYKASDDQFSQYDKEENVENNAVNKTVRKIERDPYDIASVSVAIAIDGYQD
                     LPRLPDGSYDLDPTKKPIQVPLTEDELRQAENIVKKAINFNPARGDQVAVENIMFDRT
                     KQWEQLREEFRKKEQFKRMLLAALVGILALFLGVVLFRAISRELARRRRIREEQLALE
                     QQRMREAALRAAEEEGIDVELSLEEKARLELQQNAINLARERPDDVAQLLRTWLAEE"
     gene            complement(5161..5490)
                     /gene="fliE"
                     /locus_tag="PK348_02310"
     CDS             complement(5161..5490)
                     /gene="fliE"
                     /locus_tag="PK348_02310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HXK65077.1"
                     /translation="MIQESNGITKSNIFVMKTTHPLHYSDKPQKEQDDDVAGSFMDVL
                     KSALGKVNDLQVDAEDLTQKMIYEPESVDIHTVMIAQQKAEIALSLTKAIRDEAIRAY
                     RELINLR"
BASE COUNT         1558 a         1321 c          830 g         1877 t
ORIGIN      
        1 ttttatgctc ctcatctatc acatcattca tgcaacggga aattgaacca agtacatcta
       61 tagcaggata atggttttta tgcgcaagct ttctgtccag taccacatga ccatccagaa
      121 tacccctaac agcatcagct ataggttcat tcatatcatc tgcttcaaca agtattgaat
      181 aaaagcctgt gatactccct gcaccttttt tattacccgc acgttcaagc aattgtggca
      241 ataatgaaaa tactgaaggt ggatatccac gggttgctgc aggctgaccg gctgcaagtc
      301 ccacttcacg ctgtgccatt gcaaaacgtg taatagaatc catgagtaaa ttaacatgca
      361 atcctttatc tctgaaatat tcagcaacag aatgtgccag aaaacctgcc cttaaacgca
      421 ccattggtgg ttggtcagac gtagaaataa taacaactga acgctttaaa ccttcattct
      481 gtaattcttt ttctacaaaa tcttttgctt ctcttccacg ttcaccaata agcgcaataa
      541 cactgatatc agcagccgta tttcgagcaa tcatagaaat taaagtagat tttccaacac
      601 cagaacctga aaatatacca attcgttgtc ctttacctac tgttataagc ccatctattg
      661 cccgtatccc agtaggcaat ggcgaatcaa ttatctccct ttcaagcggc gttaatggtt
      721 tattgtcaac tggatacctt tcttttaaaa aaagtttact tttaccatca atgggattgc
      781 ccaatccatc aatgaccctt ccaataagag cttctccaac cggtacaaaa agctgcgaac
      841 caactgcata aacatctgcc cccggtacaa cacctttcat atccccaatt ggcataagaa
      901 ttattttatt tttattaaaa cccacaacct cagcatacag gtaatttcca gattttaatc
      961 taatgcgaca taattcacca tactgcacat caggaccatg cgattcaatg gttaagccag
     1021 taattttttc aacgcgcccc gttgctcgca acgtatcaac ttcttcaagc acatccttat
     1081 acttggaaaa tatatcaact ttttcatgtg gtagaatctg tatcatgcaa acctcgttat
     1141 taagctatca tattggacgt gcattacgaa tagcttcttc tattgattca agctgtgttg
     1201 aaatacgagc atcaatagcc ccaacatctg tctcaataat acaaccaccc cgctcaaccc
     1261 ttgaatcttc ataaatatta actttcttca gtgactccat catctttata agttcatctt
     1321 tatgggctgt ggtcatatct aaatcagcaa aattgactcg tatatcaata cggtctctat
     1381 ctttaacacg ctgtaatgct tctttaatat tgttaataac tacttcacga cgttcaacta
     1441 tctcatcctt tattacctta cgtgcaatca tcagtatcat ttcggtcatc aacttttcag
     1501 atgacctgat tatatcatca cgaatatcta ctgctgttga aacaatggtc cccaatctat
     1561 caataaggcg cattacctcc gcctgacctt ctttgtaccc ttcttcacgt cctgcatcat
     1621 aaccacgttt ataagcttca tgttctatct ccgaaacctt caattcagct tctttgatca
     1681 tcttctctgc ttcaaattta ccgcgctcaa tctccctttc ggattctatt ttaaagcgct
     1741 ccatttcaat ctttatctgt tcacgtgcct gctgtaattc cagaaatgct gcctttttcc
     1801 cttcctcttc tatctgtgct gccttagctt tagcatcttc cagcatccgg cgcacttctt
     1861 cttctgtttc acgccggtac cgttctaact cagcttccat ctcttctata gatggaccct
     1921 gatacacctc aacagggtta ccctcttcat ctacttcaaa ttcagcatat tcctcatcgg
     1981 ttaaaacatt tttctggtat ttttcaggca actcaatctg ttttggtgcc gatataacct
     2041 gaatttcagt aggtttaaaa acaagcttag acaacgagtt cctcctcgcc ggcacgtgct
     2101 acaacaatat cgccagcttc ttcaagtttt cgtattatat taacaatttt ctgctgagct
     2161 tcttcaacgt ctctcagtct gattggcccc ataaaatcca tatcttcacg caacagtgct
     2221 gatgcacgtt ttgacatatt cctgaatatt ttttcctgaa cttcggcatc aacaccttta
     2281 agtgctttag caagatcctg tgtatcaact tcacgcaaca ctttctgaat tgctctatca
     2341 tcaagtaata caatatcttc aaaaacaaac atacgtttct ttatttcttc agcaagctcc
     2401 ggatcttctt cttcaagtgc ttcaataatt attttttcag taccacgatc aacatgggtc
     2461 aatacttcta caattgcatc aataccacct gccgaagtat aatcttcact ggctaatgtt
     2521 gaaagttttc gttcaagaac tcgctcaact tcacgtaaaa catcaggcga agtgcggtcc
     2581 atggtagcta ttcggcgcgc tacatcagcc tggatttgat ggcttaaacc cgataaaatt
     2641 gtagcagctt tctgtggatc taaataagct aaaatcaatg caattgtttg tggatgctca
     2701 ccttgaatga agtttaaaag atgactaggg tctgtacgtc ggataaaatc aaagggtcta
     2761 acctgcaacg atgacgttaa acgatttact atatcaatag ctttctgagt acccagcgcc
     2821 ctttcaagta cttcacgtgc ataatcaatg ccaccggtgg caatgaattc ctgagccatc
     2881 ataagctcct ggaattccat taaaaccttg tcccggtctt caggttcaac tttatctaaa
     2941 cgggcaattt caaacgtcag ctgctcaatt tcatcttccc tcaggtgttt aaaaatctct
     3001 gacgatacat ccgatcctag tgataccaaa aatatagcag ccttttgcct gcccgttaat
     3061 tgcgatttct tttgttgtaa catattttaa gctccacatt accccgatat acttacatat
     3121 aaatcagcca ttcttatata ttctacctac ttatagtata taccatctcc tttttaaagt
     3181 caatatcagc ttattagtat atcggtcttt caggatgtac caattacaac attattcttc
     3241 tgctaaccat gttctgagca actgtgctac atcatccggg cgttcacgtg ccaaatttat
     3301 agcattttgc tgcaattcta atcgtgcttt ttcttcaagg gatagttcta catcaatacc
     3361 ttcttcttct gcagcccgta aggcagcttc acgcatacgc tgctgttcca gtgccagttg
     3421 ttcttcacgt attctacgtc tacgggcaag ctcacgagag atagccctga ataaaacaac
     3481 acccaagaat aatgccaaaa tcccaaccag tgctgcaagc agcattcgct taaattgttc
     3541 tttttttctg aattcttcac gtaactgttc ccactgcttg gtcctatcaa acatgatgtt
     3601 ttcaacagca acctgatctc cacgcgcggg attgaaattg atggcttttt ttacaatgtt
     3661 ttccgcctgc cgcagttcat cttcagtcaa tggaacttgt attggctttt tggttggatc
     3721 aagatcatag cttccatcag gaagccgtgg taaatcctga tacccatcaa tggcaatagc
     3781 aaccgaaaca cttgctatat cataagggtc acgttcaatc ttacgcactg ttttgttaac
     3841 tgcattgttc tccacatttt cttctttatc atattgtgag aactggtcat cacttgcctt
     3901 atatccaggt ggtttgttgc cttcagtccc tgccggtccc tcagggttcc atccatgtcc
     3961 ctggaaatgc tcttttgttg attttttgga gatagttaac gagtctttta ccttgcgttc
     4021 agaatatggg gttaaaggat tgtccttttc catttctatt ggtgtatatt cttcctgttc
     4081 ttcctgaatc ttatcccagt taaaatccat attaagacgc acaatctgaa tacggtcagg
     4141 tgaaaatatc ttttctaaac cagcccttat atcttttagc atctgtacgc gcgctttttc
     4201 ttctatcttt tttctgtatt ccaaaagcgt gtattcttct tttgccttat caaactgatc
     4261 atcaaaatca gagataatct tgccatattc atcagttacc gttacatttt caggctttaa
     4321 ttttgtacct acagctcttg caactaaata ctgaataccc ttaatttcct ttttagtaag
     4381 cccttcatat cctggagcca gatacacagt aacagcagca gtataggcat tttctttatc
     4441 tgcaaatagt tcatcttcgg ttattgctat ttcaacatct gctttatcta catttttcaa
     4501 tgattcaata tgtcgtttaa tttcatcgcg caatgccctc atatacttta catttaattc
     4561 tttatccgtc tcagtccact ttgagatatc aaaaagcttc caaccaggaa tacctttagg
     4621 tatcatattt tcctgagcaa gccgtgtcag gataatttca cgctggtcag gattaaccag
     4681 tattgttgtt gtacctgatg ttgtatagtg aaaacccatc tcttcaagtt tcttggtaac
     4741 ctgcccaaac tcatcactgg gaagatctga aaataaaacc acattagctt tttgcgatga
     4801 aaccgaaaat agtacaataa aagcaataat aagtacaccg gtcactacac caataataat
     4861 tttttttgtt gtatctaatt ttgaataaat ctctttcagt tgattaaata tcttggtaaa
     4921 gaattccata ttttctttcc ctcccacaac ttaatgatta tgcgcactat gctttacata
     4981 atcacctgat tatctgttat tataaaaaca aatcacagtg gcgatagttc atagtatatg
     5041 gtacttgcaa agtgacataa tccattttca caacaaacat aataaaacac ctgtattaaa
     5101 aagtcaatta atttactaaa aatttatagt atttttatta tgtttgtaaa gcatcatttt
     5161 ttacctgaga ttaataagtt cacgatatgc cctgattgcc tcatcacgga ttgcttttgt
     5221 taaacttaaa gcaatttcag ccttttgctg tgcaatcata accgtatgaa tatccactga
     5281 ttcaggttca tatatcatct tttgtgtaag atcttcagca tcaacctgaa ggtcattaac
     5341 cttacctaat gcgcttttca atacatccat aaatgaacca gcaacatcat catcctgctc
     5401 tttttggggc ttatcgctat aatgcaacgg atgtgttgtt ttcatgacaa agatatttga
     5461 ttttgttatt ccattacttt cctgaatcat accgtacact cccagacaat attcgtgatt
     5521 caattatagg tcgctcgcca aaccccatct gttgccactg actttttcgt ctgtttaatg
     5581 taggtg
//
LOCUS       DAOSZP010000064         5560 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_12264_length_5560_cov_3.059037, whole genome shotgun sequence.
ACCESSION   DAOSZP010000064 DAOSZP010000000
VERSION     DAOSZP010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5560)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5560)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5560
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12264_length_5560_cov_3.059037"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1501
                     /locus_tag="PK348_02315"
     CDS             <1..1501
                     /locus_tag="PK348_02315"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: protein motif:HMM:NF018571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HXK65078.1"
                     /translation="DEIRNNINKDLKHVLENLILNYPVPNMIYENELIADKIISSLCK
                     IVKQVILGHLIVLGDVFDRGDEPDKIIRILNQKDIKRYLTFIWGNHDILWMGAAAGNK
                     SLIAEALRISTRYDNLAFLDRIGINITKLKEFALHTYTGEIPGNFKAKQDISRRMEKA
                     LAIIQFKLEEQTIRENPEFNMESRLWLHKLAEMLKNNDTSGLTDTQFPTIDLDNPDKL
                     SPEEEEIINDLTYQFTTSKKVRYLMEFLFEHGKLYHIHNYILNIHALIPSTHDGQFEE
                     FLGYRGKALLDHLQHRIKTIGKNYLEGKPQNPKDLALMFYLWCGSKSPFFGKNAMKTF
                     ERYFIADKSTHKEKLLYWGENLLKKEFMDKIMEEFGAERIVYGHTPVDVRKGEKIASP
                     DGRAINIDGGFSDAYLSRGHALIQTPYSLYAIILPTPEETIQARQKKEPVNLMVEIID
                     NFETPLRVKDTYTGKLLIKKRNELYEKLLQYSEHYSDMEHNTTLNYSIV"
     gene            complement(1553..1951)
                     /gene="atpC"
                     /locus_tag="PK348_02320"
     CDS             complement(1553..1951)
                     /gene="atpC"
                     /locus_tag="PK348_02320"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HXK65079.1"
                     /translation="MAMKLNCNVLTPERQIYEGQVDFAVVQAYDGEMGFLYNHAPLIS
                     ELGTGEIRLRSGDSTEYFYVDGGFVEIKNNSLIILAQEAVSKQDLKPEVLESKIKEIA
                     ALPKPETFADRLNIDIELKKLKARLKIAKR"
     gene            complement(1973..3385)
                     /gene="atpD"
                     /locus_tag="PK348_02325"
     CDS             complement(1973..3385)
                     /gene="atpD"
                     /locus_tag="PK348_02325"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002771341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HXK65080.1"
                     /translation="MAENIGKVVQVIGSTLDAEFQEDKLPAIYNALKLEVEVMGEKKT
                     LVCEVAQHLGGNRVRAVALGSTDGVKRGTPIIDTGSPITVPVGEETLGRVFNLLGDVV
                     DKGAPITVKERRSIHQEPPKFDQLEPKAEIFETGIKVIDLIEPYIKGGKTGLFGGAGV
                     GKTVVIMELINNVAMQHGGYSVFAGVGERTREGNDLWLEMKESGVINKACLVYGQMNE
                     PPGARLRVALSALTMCEYFRDLSGRDVLLFIDNIFRFSQAGSEVSALLGRMPSAVGYQ
                     PTLATEMGELQERITSTKRGSITSVQAIYVPADDLTDPAPATAFIHLDASTVLSRQIA
                     EKGIYPAVDPLESSSRILSPEIVGEEHYAVAMEVKRILQRYKDLQDIIAILGMDELSE
                     EDKLLVQRARKIERFLSQPFFVAEQFTGMPGKYVKLEDTIRSFKGLINGEYDHLPEQA
                     FYMVGTIEEAVEKAKKLAGN"
     gene            complement(3433..4317)
                     /gene="atpG"
                     /locus_tag="PK348_02330"
     CDS             complement(3433..4317)
                     /gene="atpG"
                     /locus_tag="PK348_02330"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HXK65081.1"
                     /translation="MATQREILKRIKSVKNTQKITKTMEMVSTAKMKKMQDRITMSKP
                     YGEKLETIIDHLRQTGVEDVYDPLMQERPDPNRILVLMITGNRGLCGGFNTRVIDNTI
                     NFKNKLTIEEGKEVLIYSMGKKGSNYFKFIKEPIYKSVLNPEDKFKFEDASKLGEELI
                     NLFVTGEVDEVYVSYTKIMTRSTQKATITRLLPLKPESVAIQESVEDFHVEYIFEPNP
                     YKIFSSLLPLYIKMKLYLAVLESGFSENFARRVAMKNATDAAKEMVRDLTITYNRARQ
                     AKITKEISEIVGGAAALE"
     gene            complement(4343..>5560)
                     /gene="atpA"
                     /locus_tag="PK348_02335"
     CDS             complement(4343..>5560)
                     /gene="atpA"
                     /locus_tag="PK348_02335"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002771345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HXK65082.1"
                     /translation="GRVVNPLGIPIDNKGPINSSKMRPVEFIAPGIADRQPVKEPLQT
                     GIKAIDSMTPIGRGQRELIIGDRKTGKTAIAIDAIINQKGKGVYCIYVAIGQKASTVA
                     AVVEKLEQYGAMDYTIVVAANASDPAPLQYIAPYAGCAMGEYFMYEKNGHSLCIYDDL
                     SKQANAYRQLSLLLRRPPGREAYPGDIFYCHSRLLERAAKLSDDLGGGSLTALPVIET
                     QEGEVSAYIPTNVISITDGQIYLVPGLFASGIRPAVDVGISVSRVGGNAQIKAMKKYA
                     GSLRLDLAQFRELEAFAQLGTELDPATQRQLDRGQRLVEVLKQDQYEPMPVAEQVAII
                     YAGTQGYLDKIPVSEVRKFEKEFLKFMREAHPELLDGISSTGELKDPDRFGKVVGDFV
                     ESYLAGKKADVRA"
BASE COUNT         1729 a         1176 c          928 g         1727 t
ORIGIN      
        1 tgatgaaata aggaataaca tcaataaaga tttaaaacat gtacttgaaa accttatact
       61 aaactatcct gtgccaaata tgatttatga aaatgaatta attgcagata agattataag
      121 ttcactgtgt aaaatagtta agcaggttat tctgggacat ctcattgtac ttggcgatgt
      181 atttgaccgt ggtgatgaac cagataaaat aattcgtatt ctaaatcaaa aggatataaa
      241 acggtattta acatttatat ggggtaatca tgatatattg tggatgggtg ccgcagcagg
      301 taataaatca cttattgctg aggcattgcg tataagcact cgctatgata accttgcatt
      361 cttagaccga ataggaataa atatcacaaa attgaaagag tttgctttac atacttatac
      421 aggagaaata ccgggtaatt tcaaagcaaa gcaggatata tcacgaagaa tggagaaagc
      481 acttgccatt atccagttta agcttgaaga acaaactata cgggaaaatc cagaattcaa
      541 tatggaatca aggctctggc ttcacaaatt agcagaaatg ttaaaaaata atgatacttc
      601 tggattaact gatacacagt ttcctacaat agatttagat aatcctgata aactatcacc
      661 cgaagaagaa gaaataatta atgaccttac atatcaattt acaacaagca agaaagtccg
      721 ctatttaatg gaatttttat ttgaacatgg caaactatac catattcaca attatatact
      781 taacatacat gcgttgatac ccagcacaca tgatggtcag tttgaagaat ttttaggtta
      841 cagaggaaaa gcattacttg atcatttgca acatagaatt aaaacaatag gcaaaaatta
      901 tctggaaggc aaacctcaaa atcccaaaga ccttgctctt atgttttatt tgtggtgtgg
      961 ttcaaaatcc cccttctttg ggaagaatgc catgaaaacc tttgaacgat atttcattgc
     1021 tgataaatcc actcataaag aaaagttgct ttactggggt gaaaacctgc ttaaaaagga
     1081 attcatggat aaaatcatgg aagaatttgg agcggaaaga atagtgtatg ggcatacacc
     1141 agtagatgtg cgaaagggcg aaaaaattgc atctcctgac ggcagggcca ttaatattga
     1201 cggcggattt tctgatgcgt acctcagcag agggcatgct ctcattcaga ctccgtattc
     1261 actttatgca ataattttac ccacccctga agaaacaata caggcgcgcc agaaaaaaga
     1321 acctgttaat ttaatggttg aaatcattga taattttgaa acacctttaa gggtaaaaga
     1381 tacatatacc ggcaagcttc taatcaaaaa aaggaatgaa ttatacgaaa aattacttca
     1441 atactccgag cactattcag acatggagca taatacaact ctaaattatt caattgtata
     1501 attatttgaa ctataaaaaa acgggcagtt tagtctgccc gtttttttat tatcaccttt
     1561 tagcaatctt taaacgtgcc ttcaatttct ttaattcaat gtctatattt aacctgtcag
     1621 caaatgtttc aggttttggc aatgcagcta tctctttaat ctttgattca agtacttctg
     1681 gcttcaaatc ctgtttgctc acagcttcct gagccagaat aatgagtgaa ttattcttaa
     1741 tttctacaaa accaccatca acataaaaat attctgtact atcgcctgat cgcaatcgta
     1801 tttcaccagt acccaactca gagattaaag gggcatggtt atataaaaat cccatttcac
     1861 catcatatgc ctgtaccacc gcaaaatcaa cctgcccttc atatatctgt cgttctggcg
     1921 ttaatacatt gcaatttaat ttcattgcca tagttctatc ctctacaatt tattaattac
     1981 cagcaagctt cttggctttt tcaactgctt cttctatggt gccaaccatg tagaaagcct
     2041 gctcaggaag atggtcatat tcaccattta ttaatccttt gaagcttcgg atagtatctt
     2101 ctaatttaac atatttgcca ggcataccag taaactgctc agcaacgaag aatggctgtg
     2161 ataaaaatct ttcaatttta cgtgctcgct gaacaagcaa tttatcttct tcagataatt
     2221 catccatacc taatatggcg ataatatcct gtaaatcttt atatcgctgt aatattcgtt
     2281 tcacttccat agcaactgca taatgctctt caccaactat ctcaggagat agtattcgtg
     2341 aagaagattc aagaggatca acagcaggat aaattccttt ttcagcaatc tgtcgtgaaa
     2401 gaacagtact tgcatccaga tggataaatg ctgttgcagg tgcagggtca gttaagtcgt
     2461 ctgcaggaac ataaatagcc tgaactgatg taatagaacc acgtttagta gacgtaattc
     2521 gctcctgtaa ctcacccatt tcggttgcca gagttggctg ataacctaca gcggaaggca
     2581 tacgccctaa cagtgcagat acttcagaac cagcctgtga aaacctgaat atattgtcta
     2641 tgaacagtag aacgtctcta ccagaaaggt cacggaaata ctcacacatt gtcaaagcac
     2701 tcagtgcaac acggagtcgt gctccaggtg gctcattcat ctgaccatat accaaacatg
     2761 ctttattgat aacacctgat tccttcattt caagccacag gtcattacct tcacgggttc
     2821 tttcacctac accggcaaat accgaataac caccatgctg catagcaacg ttgttgataa
     2881 gttccataat aacaactgtt ttccctacac cagcaccacc aaataatccc gttttccccc
     2941 ctttaatata tggctcaatg aggtcaataa ctttgatacc tgtttcaaaa atctctgctt
     3001 ttggctctaa ctgatcaaat tttggtggct cctgatgaat agatcttctt tctttaacag
     3061 ttattggtgc acctttatca acaacatcac ctaaaaggtt aaatactcgg cccagggttt
     3121 cttcaccaac tggtactgta attggagaac cggtatcaat aattggagtg cctcttttaa
     3181 caccatcggt agatcccaga gctactgcac gtaccctgtt accacctaaa tgctgtgcaa
     3241 cctcacatac aagggttttc ttttcaccca taacttcaac ttctaacttt aatgcattat
     3301 agattgcagg gagtttatct tcctggaact cagcatccag tgtagatcct ataacctgta
     3361 caactttacc tatattctca gccatatcta actccttaat tcatgaataa ttagtattat
     3421 atcatttacc ctttattcaa gggctgcagc accacctaca atttcagaga tttcttttgt
     3481 aatcttggcc tggcgagctc tgttatatgt tatagtcaaa tctcttacca tttcttttgc
     3541 tgcatctgtt gcattcttca ttgctactct acgtgcaaag ttttcagaaa aacctgactc
     3601 cagcactgcc agatacagtt tcattttaat atataatggt agcaatgaag aaaatatttt
     3661 atatggatta ggctcaaata tatattctac atgaaaatct tcaacacttt cctgaatggc
     3721 cacactctca ggcttcagtg gaagcagccg ggtaattgta gctttctgtg ttgaacgagt
     3781 catgattttg gtatatgaaa catacacctc atcaacttca ccggtaacaa acaaattgat
     3841 caattcctca cctaattttg atgcatcttc aaacttgaat ttatcttcag ggtttaatac
     3901 acttttatat atgggctctt taatgaactt aaaataattt gagccctttt tgcccattga
     3961 ataaataagt acttcttttc cttcttcaat ggttaattta tttttgaaat ttattgtatt
     4021 atcaattaca cgtgtattaa atccaccaca aagccctctg ttaccggtaa tcataagaac
     4081 taatattcta tttggatccg gtctttcctg cataagtggg tcgtatacat cttcaacgcc
     4141 ggtctgtcgc agatgatcaa ttatggtttc cagcttttca ccatatggct ttgacatagt
     4201 tatcctgtcc tgcatttttt tcatcttggc agtggaaacc atttccattg tcttggtgat
     4261 cttctgtgta tttttgacag actttattcg ctttaatatc tctcgttgtg ttgccacagc
     4321 ttttcctcaa aatttagtat ttttaagccc taacatctgc ctttttaccg gctaagtaac
     4381 tttctacaaa atcacccact actttgccaa atctatccgg atcctttaat tcgccagttg
     4441 atgaaatacc gtctagtaat tcaggatgag cctcacgcat gaattttaaa aattcttttt
     4501 caaactttct tacttcagat accgggattt tatccagata cccctgggta cctgcatata
     4561 tgattgccac ctgttcagca actggcattg gctcatactg atcctgtttc agtacctcaa
     4621 caagcctctg ccctctatca agctgacgct gagtagccgg gtcaagttca gtccccaact
     4681 gtgcaaaagc ttcaagttcc ctgaactgtg ccaaatcaag tcgtaatgaa cctgcatact
     4741 ttttcatggc tttaatctga gcattgccac caactcgcga tactgaaata ccaacatcaa
     4801 ccgcaggtct gataccagaa gcaaataatc caggaacaag atatatctga ccatcagtaa
     4861 tggatataac attcgtagga atgtaagcag aaacttcacc ttcctgagtc tcaattacag
     4921 gtaatgcggt tagcgaacca ccaccaaggt catcagaaag ctttgcggca cgctcaagga
     4981 gtcgtgaatg gcagtaaaag atgtcgccag gatatgcttc acgtcctgga ggtcttcgga
     5041 gtaacaatga aagctgacgg tatgcattag cctgttttga aaggtcatca tatatacaca
     5101 atgaatggcc atttttttcg tacatgaaat attcacccat tgcacaacca gcatatggag
     5161 ctatatactg tagaggtgct gggtctgaag cattagcagc aacaactatt gtataatcca
     5221 ttgcaccgta ttgctctaac ttttcaacaa ctgcagcaac ggtagatgct ttctgaccaa
     5281 tagcaacata tatacaatac acacctttgc ctttctggtt aattatagca tctattgcaa
     5341 tagcagtttt accagtttta cggtcaccaa ttataagttc tcgctggcct cgtcctattg
     5401 gcgtcataga gtcaattgct ttaatacctg tctgtaaagg ttcttttaca ggctgacggt
     5461 cagcaatacc aggagcaata aactctacag gcctcatctt gctagaatta atgggacctt
     5521 tattatctat tggaatcccc aacggattaa caactcttcc
//
LOCUS       DAOSZP010000065         5544 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_12314_length_5544_cov_3.172709, whole genome shotgun sequence.
ACCESSION   DAOSZP010000065 DAOSZP010000000
VERSION     DAOSZP010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5544
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12314_length_5544_cov_3.172709"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1786)
                     /gene="mrdA"
                     /locus_tag="PK348_02340"
     CDS             complement(<1..1786)
                     /gene="mrdA"
                     /locus_tag="PK348_02340"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HXK65083.1"
                     /translation="MQFASIRTKIIEEFRKRLFFVGIIVIAMFGIFFFQLVNLQIIQG
                     KEYSEKSRMNMENNIPIPASRGEIYDRNFAPDKQNIVLATNRPSFNIIVIPARYKEKQ
                     TLYYAIKNVCALLKLPYDEIIADIQSNNPYNRYIIQEDVPFDIVVTIATNQDKFPNIM
                     WEDAPVRVYPFANMFSHVLGYIGSLTKDEYNTYKDVGYKYYQKIGKAGIERQYDSTLR
                     GVDGYVRRIVDAKNRTEGEEIGQHPKPGYNIILTIDYDVQKTAYTALGDNRGSVIVMK
                     PATGEILSLVSKPDYDPNLIISKNNAKIITQLNNDKNKPFLNRTIQSRYPPASTFKLV
                     TAIAALEEEKWNPNVTLYCPGKYTLKGYVDRDFYCYQAHGSVNLLWAIAKSCSVYFYQ
                     LGLKLGPTTIFNYAGYLGLAEPSGIDIPGEITGFIPSKKWKLKTFGQPWFDGDTVNLS
                     IGQGFMLVTPMGMMDFISAIVNSGVVFKPHLVKEIRSNDNSSIVSVVVPEKLREIPLS
                     PTTLETIKQGMRMAVVSGTAARLGYLKVQMCGKTGTAQTRSKRQEDASQHAWFVGFGP
                     YDAEPEKVVAIVVMVEYGVAGAATAVPVA"
     gene            complement(1790..2290)
                     /gene="mreD"
                     /locus_tag="PK348_02345"
     CDS             complement(1790..2290)
                     /gene="mreD"
                     /locus_tag="PK348_02345"
                     /inference="COORDINATES: protein motif:HMM:NF016019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HXK65084.1"
                     /translation="MILMYIITGALLLASLIIQGHSSFDVLRIGGVKPDLIFIVVVYF
                     AYSFGSLYGEVTGFIGGLLHDAISNSPLGLLAFPKMALGYIIGFFGRSIFKENLFTVF
                     LLIFFASIAKGVITLILCYLFHQGSLSSITSIILPESFYNALLSPPLFFLFDKIFEQE
                     LSREGR"
     assembly_gap    2402..2411
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2412..3162)
                     /gene="mreC"
                     /locus_tag="PK348_02350"
     CDS             complement(<2412..3162)
                     /gene="mreC"
                     /locus_tag="PK348_02350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HXK65085.1"
                     /translation="MEFFIKHKTIILFILFTLFCFISLSFQSSGVTNVVEGAGNAVIF
                     PFQKLYHAVQTNVSLLWSGFSQLRNAQEELEKTRLRLQQYESVAEELEEIKRENEQLR
                     NLLGLKQRLKYDTVLATIISKDPDNWFRTIIINRGSSDGIEVNMPVVAFFGDEKAIVG
                     KIIEVRGSISRILPIISPDLKIGVVFQESRYPGLLSGYSAIGKLCVIDYVDKTATIKP
                     GDIVITSGQGGVFPQGLLVGVAVKSFPSESGA"
     gene            complement(3249..4274)
                     /locus_tag="PK348_02355"
     CDS             complement(3249..4274)
                     /locus_tag="PK348_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010574148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HXK65086.1"
                     /translation="MIFDSVYSLFSNDMGIDLGTANTLVHVKGQGIVLSEPSVVAVQT
                     STGKVLAVGHEAKRMLGRTPGDIVAIRPMKDGVIADFETVEKMIRYFIQKVHKRTTLV
                     RPRVVIDVPSGITEVEKRAVRESAEQAGAREIYLIEEALAAAIGANIPIHEPAGHMIV
                     DIGGGTTETAVISLGGMVIADSVRIAGDEFDEAIIKYMRTQYNLVIGERMAEDVKFRL
                     GNAFPEKKIETMELKGRDAISGLPRTLEIDSTEIRKALKEPVDQILDAIKHTLERTPP
                     ELAADIVERGIVLSGGGSLLKGLDKYISKETGVPVIRAENPLTCVVMGAGRFLEEIKN
                     LYRSNLK"
     gene            complement(4397..5392)
                     /locus_tag="PK348_02360"
     CDS             complement(4397..5392)
                     /locus_tag="PK348_02360"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXK65087.1"
                     /translation="MYYRYKSKQRRKITTKWVINLLLIIAAVYVVHQYHQYIFFWKYT
                     INKLQEKIEIASKQPDSTIRQQQLKDLCNAAYKYAESNPLSSDAYYLLGETYCHYAYS
                     LLNKSIYSLYDESTLQNISANIRQYFLLSIKAINKGIALGGRSSIDDNTRVIQAISLY
                     LSGYYPVNEIYNIIQKVSNIQKLSLHNIRMYSLIAIKSGHYEEGIELVKAKGVSDNIE
                     GRMFLAASYLYANQQSNAIMQYRNILQNSRDNTIIKEVHIELGKIFYRKGVYDQSIEH
                     LNAALQIDAQDVQCKLWLGKVYSAVGNVSQAKALWSEVLLLDSENAEAKRLLGIM"
BASE COUNT         1772 a         1134 c          861 g         1767 t
ORIGIN      
        1 cagcaaccgg cacagcagta gctgctcctg ctacaccata ttcaaccatg acaactatgg
       61 ctacaacctt ttccggttct gcatcatacg gaccaaaacc aacaaaccag gcatgctgac
      121 ttgcatcttc ctgccttttg gaacgtgttt gtgcagtacc agttttgcca cacatttgca
      181 ctttcagata tcctaaccgt gcagcggtac cacttactac agccattctc attccctgtt
      241 taatagtttc cagtgttgtt ggcgataaag gaatttcccg taatttttcg ggaacaacaa
      301 ctgaaactat agaagaatta tcatttgaac gaatctcttt aacaagatgg ggtttgaaaa
      361 caacccctga attaacaata gcagaaataa aatccatcat gcccataggt gtaaccagca
      421 taaaaccctg gccaatggaa agatttaccg tatcaccatc aaaccatggc tgaccaaagg
      481 tctttagttt ccattttttt gaaggtatga atcctgtaat ctcacctggt atatcgatac
      541 ctgaaggttc agctaaacct aaatagcctg cataattaaa gattgtagtt ggccctaatt
      601 ttaatccaag ctgataaaaa taaacactgc atgattttgc aatagcccac aataaattaa
      661 ctgagccatg agcctgatag caataaaaat ccctgtctac atatcctttc aaggtatatt
      721 taccagggca atataatgtt acattggggt tccatttttc ttcttccagt gcagcaattg
      781 cagtaacaag tttaaaggtt gatgcagggg ggtatcggga ctggatagtc ctgtttaaaa
      841 atggcttatt cttatcatta ttcaactgcg ttattatttt tgcattattt tttgatataa
      901 ttagattggg gtcataatcc ggcttgctta ctaaacttaa aatttcacct gttgcaggct
      961 tcataacaat aacagagccc ctgttatcgc ccaatgcagt ataggctgtt ttctggacat
     1021 cataatcaat cgtaagaata atattatacc cgggcttggg gtgttggcca atttcttctc
     1081 cttcagttct atttttggca tcaacaatac ggcgtacata accgtcaaca cctcgtaatg
     1141 ttgaatcata ctgccgttca attcctgctt tgcctatctt ctgataatat ttgtatccaa
     1201 catccttata ggtattatat tcatctttgg ttaagcttcc aatgtatcct aacacatgcg
     1261 aaaacatgtt tgcaaacgga tataccctta ccggtgcatc ttcccacata atgtttggaa
     1321 atttatcctg atttgtggcg atagttacta caatatcaaa cggaacatct tcctgaataa
     1381 tatatctgtt atacggatta tttgactgta tatccgcaat tatctcatca tagggaagtt
     1441 ttaaaagtgc acatacattt tttattgcat aatataatgt ttgtttttcc ttataacggg
     1501 cgggaataac aataatatta aaagacggtc tgtttgttgc cagtacaata ttttgtttgt
     1561 caggagcaaa atttctgtca taaatttcac cacgggatgc aggaattgga atattgtttt
     1621 ccatgttcat acgagacttt tccgaatatt ccttcccctg aattatctgc agattaacaa
     1681 gctggaagaa aaaaatacca aacatggcaa taacaataat gccaacaaaa aaaagccgtt
     1741 ttctgaactc ttctataatc tttgttctta ttgaagcaaa ctgcatatct caacgacctt
     1801 ccctggacaa ctcctgttca aagatcttat caaaaaggaa gaataatggt ggggataata
     1861 aggcattata aaatgattca ggtaaaataa ttgaagttat tgaggataat gatccctgat
     1921 ggaataaata acataatatc agtgttataa cacctttagc aatcgaagca aaaaagatta
     1981 aaagaaaaac agtaaaaaga ttttctttaa aaattgacct cccaaaaaaa ccaattatat
     2041 accccagagc cattttgggg aaagccagca aacctaaggg ggaattggaa attgcatcat
     2101 gtaatagtcc accaataaat ccagtaacct caccatataa tgagccaaaa ctataggcaa
     2161 aatatacaac aactataaaa attaggtcgg gtttaactcc acctattctc agaacatcaa
     2221 aggaagaatg tccctgtatt attagtgatg ctagcaatag tgctcctgtt attatataca
     2281 ttaaaatcat agttgtgaat catcctggat taattttaaa atctctttat ctggctcttt
     2341 tttaatgaca acaacattct caataaggtc aaaattgata tatggcttaa ctagaacacg
     2401 cnnnnnnnnn natgcaccag attcggatgg gaatgattta actgcaactc ccacaagtaa
     2461 tccctgaggg aacacaccac cctgaccaga cgtaataacg atatcacccg gcttgatagt
     2521 tgctgtttta tcaacataat caataacaca taatttacca atagctgaat acccacttaa
     2581 aaggccggga tacctgcttt cctgaaaaac aacaccaatt tttaaatcag gagaaataat
     2641 tggcaaaatg cgtgatattg aacctcgtac ttcaataatt tttccaacta tcgctttttc
     2701 atcaccaaag aatgcaacta ctggcatatt gacttctata ccatcagaac tacctctatt
     2761 gataattatc gttctgaacc agttatcagg atcttttgat attatagtag ccagaactgt
     2821 atcatacttt agacgttgtt ttaggcctaa aaggttccgc agttgctcat tttctcgttt
     2881 tatctcttca agctcttcag caacagattc atactgctgt agccgtaatc ttgttttctc
     2941 cagttcttcc tgagcatttc gcaattgcga aaaccctgac cataaaaggg atacattggt
     3001 ttgaacagca tggtataatt tctggaaggg gaaaataaca gcattacctg ctccctctac
     3061 aacgtttgtc actccagacg attggaaaga aagtgatata aaacaaaaga gggtaaatag
     3121 tataaataat ataatcgttt tatgctttat aaaaaattcc acaataaacc catttacctt
     3181 aaatataaaa taaatactcc tgcagtatat gcaagagcat ttgtatagtg aatgaaatac
     3241 agtaaaaact acttcaggtt agatcgataa agatttttaa tttcttctaa gaatctaccc
     3301 gctcccatga caacacaggt taatggattc tctgctctta taaccggtac tcctgtttct
     3361 ttgcttatat acttatcaag acctttaagc aatgatccac cacctgatag tacaatccca
     3421 cgctcaacta tgtcagcagc aagctcaggg ggagtgcgtt ctaaggtatg tttaatagca
     3481 tctaaaatct gatctacagg ctcttttaat gcttttcgta tttcagtaga atctatctcc
     3541 aatgtccgtg gcaaacctga gatagcatca cgtcctttta attccatagt ttcaatcttc
     3601 ttttcaggga acgcattacc taacctgaat ttaacatcct cagccattcg ctcgccaata
     3661 accaggttgt actgagttcg catatatttt ataattgcct catcaaactc atcaccagca
     3721 atccttactg aatctgcaat aaccatacca cccagggaga taacagcagt ttctgtagta
     3781 ccaccaccaa tatcaactat catgtgcccg gctggctcat gaattggaat attggcacct
     3841 atagctgctg ccagtgcttc ttcaatgaga tatatttccc gggcaccagc ctgttcagca
     3901 gattctctga ctgcacgttt ttcaacttcg gtaatacctg atggtacatc tataactacc
     3961 cttgggcgaa ccagggtagt acgtttatgc actttctgta tgaaatatcg tatcattttt
     4021 tctacagttt caaaatcagc aataacacca tctttcatag gtcgtattgc aactatatca
     4081 ccaggagttc ttccaagcat cctctttgct tcatgtccaa ctgctaaaac cttacctgtg
     4141 ctggtctgga cagcaacaac agaaggttca ctcaaaacaa taccctgccc ttttacatga
     4201 accagcgtgt tagcagtccc taaatctatc cccatatcat ttgaaaataa actataaaca
     4261 gaatcgaaga tcataataag tttacctttt agtcaaaaaa ttgattatcc gaatattatg
     4321 tgcaattgaa tgatacttta cggccgtttt tgtcaagcga ttatcattaa atttttttat
     4381 caaaaattaa aaaagattac attatcccca gtaatcgctt tgcttcggca ttttctgaat
     4441 caagcaaaag gacctcactc cacaacgcct ttgcctgaga tacattccct accgctgaat
     4501 atacctttcc cagccaaagt ttacattgta catcctgagc atcaatctgt aaggcagcat
     4561 taaggtgttc aatagattga tcataaactc cttttctata aaatatcttt cctaattcaa
     4621 tatgaacttc tttaataatg gtattatccc tggaattttg aagtatattt ctatactgca
     4681 taatggcatt gctctgctga ttggcataaa gatatgatgc agctaaaaac attcttcctt
     4741 caatattatc tgaaacccct tttgctttta caagctcaat cccttcttca taatgaccgc
     4801 tttttatggc tataagcgaa tacattctga tattatgtaa ggataacttc tgtatatttg
     4861 aaactttttg aataatgtta tatatttcat taacaggata atacccactt aaatacaatg
     4921 atatggcctg aataaccctt gtattgtcat caatggaaga ccggccgccc agagctatcc
     4981 ctttatttat ggcttttata gataaaagaa aatactggcg gatattagca gagatattct
     5041 gcaacgtcga ttcatcatat aacgaataaa tggatttatt taaaagacta tatgcataat
     5101 gacagtatgt ttcgcccagt aaataatatg catccgaact caacggattg ctttcagcat
     5161 atttatacgc tgcattgcag agatctttta actgctgctg gcggatggta ctatctggct
     5221 gttttgaagc tatctcaatc ttttcctgta atttatttat tgtatatttc cagaagaata
     5281 tatattgatg atactgatga acaacataca cagcagcaat tattaatagt aaattaatta
     5341 cccatttcgt tgtaattttt cttctctgtt ttgatttata gcgatagtac atggtaaaaa
     5401 tcacccttag cattaagatt tggtaaaatt ggaatattct ttttcaatat tcagtaaaac
     5461 atcagactct tcattaagaa ctatacaatt aacagtattt ttacctttta ttgaaaataa
     5521 catgtaatta tcgtgtgctt tcat
//
LOCUS       DAOSZP010000066         5517 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_12391_length_5517_cov_3.147016, whole genome shotgun sequence.
ACCESSION   DAOSZP010000066 DAOSZP010000000
VERSION     DAOSZP010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5517
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12391_length_5517_cov_3.147016"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..744
                     /locus_tag="PK348_02365"
     CDS             <1..744
                     /locus_tag="PK348_02365"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXK65088.1"
                     /translation="SIRISSGNTSLHFDPFISMNKSLQYASIDDYSGATDIFITHGHF
                     DHIYNIPEIWDLSKCTIYCGIQVADVLLKQGVLPDYIRIIDHGQVVLIPPFIIKIYKS
                     EHIRFDIPLIFKTLINKRVLQYFGDFKQIIALNKTMPAGTIFAYEIVCDDKSILHFGS
                     LNYSPTIEYPYKPAMLTLPFQGRSDIDHYAIRFIKKFNPQSIFLHHFDDTFPPVSSHV
                     DTTQFVEAVTKAFPHITVIIPQYGIPYTL"
     gene            complement(937..3591)
                     /locus_tag="PK348_02370"
     CDS             complement(937..3591)
                     /locus_tag="PK348_02370"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXK65089.1"
                     /translation="MDFKSTFNEQIVSMLNIFVNEQKKRFPSNEVLTIDLHCHDHNSN
                     IPDEQLGRILGIPETWLPTEDLLTTLKNHGCDTYTVTNHNNARSCYELLDKGHDVLVG
                     AEFSCTVPDYKVGIHVLAYGFTPEKEIKLNKLRTDIYKFLDYTLENDIPTIWAHPLYH
                     YKSKGIPPMEFFDKMALLFERFEVINGQRDSWQNMLVKQWIESLTQDKLEELSHKFKI
                     KPDRYCTTPYTKYMSGGSDSHMGIFTGLTGTYLHIPDLAERLKTEKRSALALEAIKNG
                     AMAPFGTHNDSEKMAISFIDYFCQIGMNMKDPGLPHILLHKGDVWDKLSALAITNAFH
                     ELKRHKTTLNFLELFHKSLIGKTFSKTKRLYVSKDYKKVFDIAYKLSRYTKTRKKDDV
                     TFYTQSVYGIYKELVQLLMKRSDKHINELINNSRFKSKEFESFFENIEIPTHFRKYIE
                     GNEKPNGNGSARVNLPKLFDELSFPFLGSAVIATAFFTSARVMYKARPLLYEFAKAHN
                     TLKHPERTLWLTDTFADSNGVALVLQSMLKEIQTRDLPIDIITISSTLQPQDHLIVIR
                     PLKEFTLPFYEQQPLRIPDILEIHNIFKQGEYSKIIASTEGPMGLAALWLKYAYSVPA
                     YFYVHTDWMMFAEQTLKLTDENLHNFRRLLRTFYKGFDGLFVLNNDHRKWLTGKDMGF
                     NPSQVHLTAHWVEETFKPYKINKEDIFGVSNTTPVILFAGRLSEEKGVMDIPKVMEIV
                     KQKHPDAKVAFVGIGPKEQELRKLLPDAIFLGWVQHDTLPKIYSAADVLVLPSRFDTF
                     GCVVLEALSCGLPVLAYNTKGPKDIINNGLNGYLVKNEIEMGQKIIELLDNPLLLNLF
                     KNQSLLRAHEFSPDAIIKQFINDLNSAA"
     gene            4000..4476
                     /locus_tag="PK348_02375"
     CDS             4000..4476
                     /locus_tag="PK348_02375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65090.1"
                     /translation="MNAETTTCISLTHDKILKEICKKYNLSITTIVVLMVKFAVERQK
                     LSIISNRNVKYRNKNCSIWRRIHLQLSPNDYEFLMDVKKIWKISVAKMIEYCIDNVLF
                     ELQEKVLEKNKTDNYLYNNYHFEIGEEEGLTYYLIYWGLPLKLLKKLTLQNSILCP"
     gene            4925..5161
                     /locus_tag="PK348_02380"
     CDS             4925..5161
                     /locus_tag="PK348_02380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65091.1"
                     /translation="MNTGLLLTIIAAVSFVILIYMLIKQVMKIVVIIAVLFIMLAGYI
                     YIKNGSLPANVKGYVEEGKIAIDQLHQSYLKLIK"
     gene            5266..>5517
                     /locus_tag="PK348_02385"
     CDS             5266..>5517
                     /locus_tag="PK348_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020001669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HXK65092.1"
                     /translation="MKTQTKDTALNSMQIVCFNIGKEEYGIEILKVQEILKLPAVTPL
                     PKSADYIMGVIDLRGKVIPIIDLGVRFGISEKGAKNKRAI"
BASE COUNT         1767 a          881 c          973 g         1896 t
ORIGIN      
        1 tcaattagaa tatcttcagg caacacttct ttacactttg atccattcat atccatgaat
       61 aaatcattgc agtatgcttc cattgatgat tattcaggtg caacagacat atttattaca
      121 cacggccatt ttgatcatat atataacata cctgaaattt gggatctgag taaatgcacc
      181 atatattgtg ggatacaggt tgccgatgtt ttgcttaaac agggtgtttt gccagattat
      241 atacgaatta ttgatcatgg acaggttgtg ttaatcccgc cctttattat aaaaatatac
      301 aaaagtgagc atatacgttt tgatattcct cttatattca aaactcttat caataaacgg
      361 gtattacaat attttggtga ttttaaacaa attatagctc tgaataaaac aatgcctgcc
      421 ggtacaatat ttgcttatga gatagtatgt gatgataaat ctatactaca ttttggcagt
      481 ttaaattatt cccctactat cgagtatcct tataagccag ccatgctgac acttccgttc
      541 caggggcgtt cggatatcga tcactatgcc atacgcttta taaagaaatt taatcctcaa
      601 tcaatatttt tacatcattt tgatgataca ttcccaccag tgtcatcaca tgtggatacc
      661 acacaatttg ttgaagcagt tacaaaggct ttcccgcata ttaccgttat tatcccccag
      721 tatggcattc cctatacatt ataaactatg ataaatcgcc aactttctgt agaatagttg
      781 cttcataggg gtaacactgc aatcgcaggt cagcaaagtg ttcaaattta ctcctatgag
      841 tggaatacag tactttccac tgcgcttcat agcgatggtt tagcattgtt gggttattat
      901 attaccccgt gcttccacca tatatgcttt tgttgattat gctgcgctgt taaggtcatt
      961 gataaattgc ttgatgattg catcagggct aaattcatga gcacggagca atgattgatt
     1021 tttgaagagg ttcagaagca gaggattatc cagaagttct atgatctttt ggcccatttc
     1081 aatttcattt ttcaccagat atccatttaa tccgttgttg atgatatctt tgggtccttt
     1141 tgtgttgtaa gcaagtacag gcagtccgca tgataacgct tcaagcacta cacatccaaa
     1201 tgtatcaaat cgtgatggca atactagaac atctgctgct gagtatatct ttggtaaggt
     1261 atcatgctga acccatccca aaaagattgc atctggcaaa agctttctta gttcttgttc
     1321 ttttggccca atgccaacaa atgcaacttt tgcatcagga tgtttttgct taactatctc
     1381 catgactttt ggtatatcca tgacaccttt ttcttctgaa agtcttcctg caaaaagtat
     1441 aacaggtgtt gtgttgctta ctccaaaaat gtcttctttg tttattttat atggcttaaa
     1501 ggtttcttca acccagtgtg cagttaagtg tacctgtgaa ggattaaaac ccatgtcttt
     1561 acctgtcaac catttgcggt ggtcgttatt aagaacaaac agtccatcaa atcctttata
     1621 aaatgtgcgc aataaacgtc tgaaattatg taagttttca tcggttagct ttaatgtttg
     1681 ttctgcaaac atcatccagt cagtatgcac gtagaagtat gcaggaacag aataagcata
     1741 ttttaaccac agtgcagcta atcccattgg gccttccgtt gaagcaataa ttttactata
     1801 ttcaccttgt ttgaagatgt tatggatttc cagaatatca gggattcgca aaggttgctg
     1861 ttcatagaat ggaagggtaa attcctttaa tgggcgaata acaattaagt gatcctgtgg
     1921 ttgaagtgtg ctgctaatag taataatatc aatgggtagg tctcgtgttt gaatttcttt
     1981 aagcatagat tgcagaacaa gagcaacacc attggaatct gcgaaagtat cagtaagcca
     2041 tagtgtacgt tcggggtgtt taagcgtatt gtgtgctttt gcaaattcat aaagtagtgg
     2101 tcgtgcttta tacattactc gggcactggt aaaaaaggct gttgcaatga ccgctgagcc
     2161 caaaaatggg aaggacaact cgtcaaaaag ttttggcagg ttaactctgg ctgaaccatt
     2221 accattaggt ttttcattgc cttcaatata ttttctaaag tgggtaggga tttcaatatt
     2281 ctcaaagaat gattcaaact cttttgattt aaatctgctg ttattaatta attcgtttat
     2341 atgtttatca gaacgtttca tgagtagttg aacaagctct ttatatattc catatactga
     2401 ttgtgtatag aatgtcacat catctttttt tctggttttt gtatatcgtg ataatttgta
     2461 ggcaatatca aaaacctttt tataatcttt acttacatac aatcgttttg ttttggaaaa
     2521 tgtttttcca attaatgatt tatggaaaag ctctaaaaag tttaacgtgg ttttatgccg
     2581 tttcaattca tggaaagcat tggttatggc cagtgcagat agcttgtccc aaacgtcgcc
     2641 tttgtgtagc agaatatggg gaaggccagg gtctttcata ttcatgccta tctggcaaaa
     2701 gtaatcaatg aaagaaattg ccatcttttc actgtcatta tgtgtaccaa aaggtgccat
     2761 tgcaccattt tttatggctt ctagtgccag tgctgaacgt ttttcggtct ttaatctttc
     2821 agctaaatct gggatatgca gatatgtccc cgttaagcca gtgaagattc ccatgtgact
     2881 atcgctgccc cctgacatat acttagtgta gggtgttgta caatatctat ctggttttat
     2941 tttgaatttg tgcgataact cttctaactt atcctgtgtt agtgattcaa tccactgctt
     3001 aaccagcata ttctgccagc tatcgcgctg tccattgata acttcaaagc gttcaaataa
     3061 aagggccatc ttatcaaaaa attccatggg tggtatgccc tttgatttgt agtgatacaa
     3121 tgggtgtgcc cagatagttg ggatatcatt ctcaagagta taatctaaga atttgtatat
     3181 atcagtacgg agtttattaa gttttatctc tttctcagga gtaaagccat atgcaagcac
     3241 atgaatacct actttgtaat caggtactgt gcagctaaat tcagcgccaa caagtacatc
     3301 atgtccttta tcaagaagct cgtaacatga tcgggcattg ttatgatttg ttactgtata
     3361 agtatcacaa ccatgattct tcagagttgt aagtaaatct tcggttggta gccatgtttc
     3421 ggggattcct aatattcttc ctaactgttc atcagggata ttgctgttgt ggtcatggca
     3481 gtgaaggtca attgttaaca cttcatttga aggaaagcgt ttcttttgtt cgtttacaaa
     3541 aatgtttagc attgaaacaa tttgttcatt aaatgtactt ttaaaatcca tatctcctct
     3601 taaagtttct atatccttaa atctcgatga taatatgacg tggatgtgta aagattttat
     3661 gaagtttatg tttagttgtg gtgaaaaaat cttgattatt tatattaggt tttaatattg
     3721 ttaaatgggt gagtatataa agtaataaaa aataaaaact ttaaaagatg atatccttat
     3781 taaaattgtt taatctatgc gttgatgaca ctgtgggaag gaaatgttaa aatgtggttg
     3841 tgatatttgc gggtaaatat cgacaataaa tcatataatt aatgcaaaaa atattaaata
     3901 ttaatgtcaa cataccaaag tagaagttgt tgaaaaagtt aaatttaatt ttgtttaaaa
     3961 aagcaatagt aacataaata aacataaatg gagtacttta tgaatgctga aacaacaacc
     4021 tgtatttctc ttactcatga caaaatttta aaggaaattt gcaaaaagta taatctttct
     4081 atcacaacaa ttgttgtatt aatggttaaa tttgcagttg aaagacagaa attatcaata
     4141 atcagcaaca gaaacgtaaa gtatagaaat aaaaattgtt ccatctggag aagaattcat
     4201 ttgcaattaa gtccaaatga ttatgaattt ttaatggatg ttaagaaaat atggaagata
     4261 tctgttgcta agatgattga atattgtata gataatgtac tttttgaatt gcaggaaaaa
     4321 gttttagaaa aaaataagac cgataactat ctatacaaca actatcattt tgaaattggt
     4381 gaagaagaag gactaacata ttacctcatt tactggggat taccgctaaa attactgaaa
     4441 aaactcacct tgcaaaactc aatactttgc ccatagtaat aatatacaca tcaatactat
     4501 actttacttg acatatttta ctattgctat atcaatataa ttaaatttta ccagttgcaa
     4561 caaatattct cgtattatct ttttatttat tgggagagac agagactatt gcttaattat
     4621 acaaaactta taaatgctat agtataatgt tctatgcatc aaaatatgtt tagtcaatac
     4681 aacagggtag atgtatgcga gtaaaagtaa taatcatttc aattattctt tttttgtttc
     4741 ttataataat attacagaat acccaaccgg tttcattaac tttacttgcg tggaatatca
     4801 ctttgccatt tattgtaatg ctttttgcta ctttaataat tggattggta tccggtatgg
     4861 tactgagttc tttggcattc cgaaaaaaac gtaaaagtat tataaaagta gaaaacagaa
     4921 aatcatgaat actggattat tattaacaat aatagcagca gtcagctttg ttattcttat
     4981 atatatgctt ataaagcagg tgatgaagat tgttgtcatt attgcagtac tgtttataat
     5041 gttggcagga tacatatata ttaaaaacgg ttcacttcct gctaatgtaa agggatatgt
     5101 agaagaaggg aaaatagcaa ttgaccagtt gcatcaatcg tatttaaaat taataaaatg
     5161 agttctcatg agctatcgcc aaaaaataaa aattttaact tgaaaaattg agttattatt
     5221 actactattg tgaaaatgag ttaaaattgc attaaggatt tcagtatgaa aacccagact
     5281 aaagatacag ccctcaatag tatgcagata gtatgcttca atattggcaa ggaagaatat
     5341 ggcatagaaa ttcttaaagt acaggaaatc cttaaactgc cagctgttac accactgcca
     5401 aaatcagcgg attatattat gggagtcatt gatttacgtg gaaaggttat accaattatt
     5461 gatctgggtg tgcgttttgg tatatctgaa aagggtgcaa aaaataaacg agcaata
//
LOCUS       DAOSZP010000067         5516 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_12393_length_5516_cov_3.150705, whole genome shotgun sequence.
ACCESSION   DAOSZP010000067 DAOSZP010000000
VERSION     DAOSZP010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5516
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12393_length_5516_cov_3.150705"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(67..1173)
                     /locus_tag="PK348_02390"
     CDS             complement(67..1173)
                     /locus_tag="PK348_02390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65093.1"
                     /translation="MITINFEESAKTIISLLQQQENIAIIIKGSPDPDALASSYFFWK
                     LCGILHVKATIFGFMPVSLPSNKEFISVFNIPLRVLADIKEIKLHNYTGYIVCDYQTA
                     VLEENFTTTPCIIHLDHHSPAEHDVPARFRILTDQVSSTSTLCALLMQHISLVSLEDV
                     SSLATALYVGIKTDSDGFKHVGEYDTMALEYIKPYCNMAVIEQIESTPLTPQALSLLQ
                     KAMDNAIVYKDWLIAGVGFVEERYRDTIAIIADYLLQDNPVELVVVYGAVYHNNHIKL
                     DIDASLRTRSENFDLNELIKNITADGGGRKYKGAFQVHIDYFVHCPDQDKLWEIIEIT
                     TNQILKNLRDSSLKIVLQSKFKNFKKILKHLFRL"
     gene            complement(1214..3310)
                     /locus_tag="PK348_02395"
     CDS             complement(1214..3310)
                     /locus_tag="PK348_02395"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HXK65094.1"
                     /translation="MVNVLKRFSYAVNRWFSFIALLTAGMISCLLVPFFIPNIEITEL
                     TRWYFALIIIINQAFFHYTQIFPRWEKKSPLFIIIISAIPGVITFLYTVATNSIILSA
                     SFDGLHIQFVYGQFIVLYLIVFIFYIMGTFLTLYYKVRRLENTSFRYQLFYVFMGNHI
                     GALIILISFIIMPYVFKRFEYHSLGISLAALMLLYINNYAISDERYLNFKEFYLKIVY
                     YGVVLVFTVIPTYWILVNSYRYFLAGGQIPAVAVAFINFVYLIFFTRIIQPYLEKWIK
                     REYAGLEKRFNEFIQALSQVEEFEGDPTYWDRFFTTTMDSLEYLFNVTSASFLMYNEP
                     EKAYILSHTIGQPPQVKRISADDSLVQLCQIAKTTIHQSLFYTDEIIKPYDDVLALVK
                     NNSIEVILPCFDQLGKLIGILFIGPHKKGRPYTFNEIEFMELYRVKFNSALVNSLQIE
                     EVKSKQIGEHDNLVVSAIKNSIIPRQMPYIDHIRISSFFMNNSNLGGEFFNAKVLHKN
                     ALAFLISDCSYSGVDAAVLALELYAAFESLSPTYDTPEKIMQLLNWVVCTSKFTERYA
                     TSFCFTFNSELLSLKYINAAFNPLIIYEIQNDSFIELDTKGIPIGIEKDFIYESKEYV
                     IKGPCIGCIYSNGITGAVNQTGQPYSIGRIKDLIRLHNSESPALIIRRISSDIDTYTS
                     NTAVKEDISIIIFRIE"
     gene            complement(3480..4025)
                     /locus_tag="PK348_02400"
     CDS             complement(3480..4025)
                     /locus_tag="PK348_02400"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXK65095.1"
                     /translation="MDNKSSDCIAQCYILYGNYVKSIIARFYSDKDEIEDIFHDVFLK
                     LLSAGFCGDPHSHKTRNYIRKATRHVCISRMRKEIARQKYCNNSNPDSMIDIETVTDM
                     QTMDIGETVIDGMIVSTLYDVIDELEHDEQNLIFNKYFYNKKYFQLAAEQGVSYHVIK
                     RKLLAVNKKLKHKLMNTIAYQ"
     gene            4390..5073
                     /locus_tag="PK348_02405"
     CDS             4390..5073
                     /locus_tag="PK348_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008731394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HXK65096.1"
                     /translation="MHKPPLTMQTTTLWNYPSQHYGNKQQGDQNYPGATPSYIIWNLL
                     MRYTKKGDLVIDPMCGSGTTIDVCNDTGRKCLGYDIHPYRTDIIKADARALPLENNKA
                     DFVFIDPPYGDNITYSDEKDCIGKLKATEKEYFTAMEQVIQEIYRILKHERYMGLYVC
                     DYYNKKDGFVPIGFHLFSLLTHYFTPIDIIAVVRHNRTLQMGNYHKAALEGNFLLRGF
                     NYLFIMYKK"
     gene            complement(5075..>5516)
                     /locus_tag="PK348_02410"
     CDS             complement(5075..>5516)
                     /locus_tag="PK348_02410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65097.1"
                     /translation="YIIAGLPGATVKTCIDDILFLASLPVMIGFSPLYMLPGVRLFES
                     MGVPDDRRLCRGSALYSFGEGFSREDIAALWKLCRFINRLKIADAINDEVRVHYEFYK
                     KACNDHVWHYRDEKGLWHEGFSFSASLPECFHICDINGTTRLIG"
BASE COUNT         1866 a         1032 c          853 g         1765 t
ORIGIN      
        1 tatatcgtat gaacttggct tccaattcag tctgttattt taatataacc agatcttcat
       61 atatgattat aagcgaaata aatgtttaag tatttttttg aagtttttaa atttactctg
      121 taacacaatt ttcaagctac tatctcttag attttttaat atctgatttg ttgttatttc
      181 tattatctcc cacaacttat cctgatcagg gcaatgcaca aaataatcaa tatgaacctg
      241 aaatgctccc ttgtacttgc gtccgccacc atcagcagta atatttttta taagctcatt
      301 caaatcaaaa ttttcacttc ttgtcctcaa cgatgcatca atatccaatt taatgtgatt
      361 attatgatac actgccccat atactactac aagctcaact ggattatctt gtaaaagata
      421 atccgcaatt atggcgatag tatcacgata tctttcttca acaaatccaa ccccagcaat
      481 aagccaatct ttataaacaa tcgcattatc cattgctttc tgtaacaggc tcaatgcctg
      541 aggagttaaa ggagtacttt ctatctgttc aattactgcc atattgcaat atggctttat
      601 atattccagt gccattgtgt catattcacc tacatgttta aaaccatcag aatctgtttt
      661 aatgcctaca tatagtgctg ttgccagact ggaaacatct tctaatgata ccaacgatat
      721 atgctgcatt aataatgcac agagtgtgct tgttgacgat acctgatcag ttaaaatgcg
      781 aaaacgtgca ggaacatcat gttcggctgg agaatggtga tctaaatgga ttatacaggg
      841 ggttgtagta aaattttctt ccagaacagc tgtctgataa tcgcaaacaa tataccctgt
      901 gtagttatgt agtttgattt cttttatatc tgccaaaacg cgtagtggta tattaaaaac
      961 tgatataaat tctttatttg atggcaggga aacaggcata aatccaaata tagtagcttt
     1021 aacatgtaat attccacata gtttccagaa aaaatatgac gaagctagtg catccggatc
     1081 cggagaacct tttataataa ttgctatatt ttcctgttgc tgtaacaacg atataatggt
     1141 tttggccgat tcttcaaaat taatagtaat catgataaca ctactataat tagatatatt
     1201 ttattgggag taatcattct attcggaaga taataatact gatatcttcc ttaaccgctg
     1261 tattgcttgt atatgtatca atgtctgaag aaatacgcct gattataaga gccgggcttt
     1321 cactgttgtg gagccttatg agatctttaa ttcttccaat ggaatacggt tgccctgtct
     1381 gattcacagc accggtaatg ccattagagt aaatacaacc aatacacggt cccttgataa
     1441 catattcctt tgattcataa ataaaatctt tttctatccc aatggggatc cctttagtat
     1501 ctaactctat aaaactatca ttttgaattt catatataat caagggatta aacgctgcgt
     1561 taatatattt cagtgatagt aattccgaat taaatgtaaa acaaaatgaa gtggcatacc
     1621 tttcagtaaa tttggatgta cataccaccc agttcaataa ctgcataatt ttttccggcg
     1681 tgtcatatgt gggagataaa gattcaaatg cagcataaag ctccaatgcc agtactgcag
     1741 catccactcc actataggag caatcggata ttaaaaaagc cagtgcattt ttatgcaaaa
     1801 cctttgcatt gaaaaattcc ccccctaaat ttgaattgtt cataaaaaat gagcttatcc
     1861 tgatgtggtc aatatagggc atctgccggg ggataattga attttttata gcactcacaa
     1921 ctaaattatc atgctcacca atctgctttg actttacctc ttcaatctga agtgaattaa
     1981 caagtgcact gttaaatttt actctgtaca actccataaa ttcaatttca ttgaatgtat
     2041 atggcctacc ctttttatga ggtccaataa aaagtatccc tatgagtttg cccaactggt
     2101 caaaacatgg gagaataact tcaatagaat tgttcttcac tagtgccagt acatcatcat
     2161 atggctttat tatttcatcg gtgtaaaata atgattgatg tatagttgtt tttgctatct
     2221 ggcataattg aaccagtgaa tcatcagcag atatcctttt tacctgaggc ggttgtccta
     2281 tagtatgact taaaatatat gccttttcag gttcattata cattaaaaat gaagcagatg
     2341 tgacattaaa taggtattcc agtgaatcca ttgtggttgt aaagaatcga tcccagtatg
     2401 tgggatcacc ttcaaattcc tcaacctgtg acaatgcctg gataaattca ttgaaccgct
     2461 tttctaatcc tgcgtattcc cttttaatcc atttttctaa atatggctgt ataattcgag
     2521 taaagaaaat taaatatacg aaatttataa aggcaacagc aactgcaggt atctggcctc
     2581 ctgccaaaaa ataacggtaa ctgttaacaa gaatccaata tgttggaatt acagtgaata
     2641 ccagcacaac accataataa acaattttaa gataaaattc tttaaagtta agatatcgct
     2701 catcagagat agcatagtta ttaatatata ataacatcaa agctgccaag gatattccta
     2761 aactatgata ttcaaagcgt ttgaaaacat agggcattat gataaaagat atgagtatta
     2821 tgagtgcacc aatgtgattt cccataaaca cataaaaaag ctggtaccga aaagaggtat
     2881 tctctaagcg tcgtacttta taatacaagg tgagaaacgt tcccatgata taaaaaataa
     2941 aaacaatcag atacagtact ataaactgac catacacaaa ctgaatatgg agaccatcaa
     3001 aagaagcaga aagaatgata ctattggttg ctacagtata caaaaaagta atcactcctg
     3061 gaattgccga aattataata atgaacagag ggcttttctt ctcccagcgt gggaatatct
     3121 gcgtataatg gaagaaggcc tgattaataa ttataattag ggcaaagtac cagcgggtta
     3181 gctcagttat ctcaatattt ggaataaaaa aaggcactag caggcatgaa atcatccctg
     3241 cagtaagtaa tgctataaac gaaaaccacc tgttcactgc atatgaaaac cgtttcagaa
     3301 cattaaccaa aactacatac aacgcataaa atattgtaag aaaacaacac aagataatta
     3361 tatatttcaa agcaaaacct tccgatatct atgatttaat atatatttaa actaactctg
     3421 aatacttcta taatagatat cgataaattt caagctaaat aataaaacat ctgtaagctt
     3481 tattgatatg caatggtatt catcaattta tgctttagct ttttattaac tgccaaaagc
     3541 tttcttttta ttacatgata tgaaacaccc tgctcagcag ccaattggaa atattttttg
     3601 ttataaaaat atttattaaa tatcaaattc tgctcatcat gttcaagttc atcaataaca
     3661 tcatagagag tgctcacaat catgccatca attacagttt cgccaatatc catggtttgc
     3721 atatcagtga cagtttctat atctatcatg ctatcaggat tactattgtt acaatacttt
     3781 tgccgcgcta tctctttcct catacgggaa atgcatacat gcctggtggc ctttctgata
     3841 tagtttcttg ttttgtgtga atggggatca ccacaaaagc cagcgctgag taatttcaaa
     3901 aaaacatcat gaaatatatc ttcaatttcg tctttatctg aataaaacct tgcaataatg
     3961 cttttaacat aattgccata aagaatataa cattgagcaa tacaatcaga acttttgtta
     4021 tccatatgcc tctcccaaaa tattccttat tttttgtatt attattttta taactcttct
     4081 ttccaaaaaa ctaaatattg cacatccctg cttacaaagg ttgcgggaat gttcacacca
     4141 ttgttccaga attatttaaa taaataaatg atatttttta tactttcttt agcattaata
     4201 gtgttttgtt tcttctcatt ctgtgatata tctgaaattg agaaaatttt tctctaccag
     4261 gaattcacaa catagaaatg cattacatag tatatagttt aatatcccta tgctattttt
     4321 tagtattaat tgcttaaaat aattgatata tttatttact ggtatagttt actatcaagg
     4381 gagcagtata tgcataagcc accattaact atgcaaacca caacgttgtg gaattatcct
     4441 tcacagcatt atgggaataa gcaacagggc gaccagaact accctggagc cacaccgtca
     4501 tatatcatct ggaatcttct catgcgctat acaaaaaagg gggatcttgt tattgatcct
     4561 atgtgtggca gtggtaccac cattgatgtt tgtaacgata caggaagaaa atgcctgggc
     4621 tatgatatcc atccctaccg tacagatatc atcaaagctg atgcacgtgc attgcctctt
     4681 gaaaacaata aggctgactt cgtcttcata gacccaccct atggtgataa catcacctat
     4741 tctgatgaaa aggattgtat aggcaaactg aaagcaactg aaaaagagta ttttactgct
     4801 atggaacagg ttatacaaga gatatacaga atattaaagc atgagcgcta catgggctta
     4861 tacgtatgcg actactacaa taaaaaagat ggttttgttc ctattggatt ccatttgttt
     4921 tcactgttga cacactattt cacacccatt gatattatag cagtagtacg ccataacagg
     4981 acacttcaga tgggaaacta ccataaagca gccttagagg gaaatttttt gttacgagga
     5041 tttaattatc tttttatcat gtataaaaaa taaatcagcc aatcagcctt gttgtaccat
     5101 tgatatcaca tatatgaaaa cattcaggaa gggatgcaga aaacgaaaag ccttcatgcc
     5161 acaatccttt ttcatcccga taatgccata catggtcatt gcatgctttt ttgtaaaact
     5221 cataatgtac gcgcacctca tcattaattg catcagcaat tttaagcctg ttaataaaac
     5281 ggcataactt ccacagtgct gcaatatctt ccctgctaaa accttcacca aatgaataca
     5341 aagcactgcc tctgcacaat cgcctgtcat caggcactcc catactttca aatagtcgta
     5401 cccctggcag catatagagt ggggaaaatc caatcatcac aggcaatgaa gctaaaaaaa
     5461 gaatatcatc gatacatgtt ttaacagtag caccaggcaa ccctgcaatg atatac
//
LOCUS       DAOSZP010000068         5508 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_12406_length_5508_cov_3.189804, whole genome shotgun sequence.
ACCESSION   DAOSZP010000068 DAOSZP010000000
VERSION     DAOSZP010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5508
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12406_length_5508_cov_3.189804"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /locus_tag="PK348_02415"
     CDS             complement(<1..274)
                     /locus_tag="PK348_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010420073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HXK65098.1"
                     /translation="MDSMLFWIAPVGSIIALLFAFIFYKMVMKADKGNETMVEIAQAV
                     REGAFAYLKQQYKVVSLVFVILVILLSILAYYGIQNPFVPFAFLTGG"
     gene            511..1353
                     /locus_tag="PK348_02420"
     CDS             511..1353
                     /locus_tag="PK348_02420"
                     /inference="COORDINATES: protein motif:HMM:NF015436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HXK65099.1"
                     /translation="MASDEQKPKKTNNNDAVSLLKQKFFKFIHKKNISVPVDKLAIQK
                     QEMIQGILSLSTKNVREVMIPRVDINAVEDTIQLKELVKRVIDEGHSRIPVYSGTIDN
                     ITGILYVKDLMPFIIDRRMKFNIKKLLHKPYFVPETMTLDELLLQFQKQNLHLAIVVD
                     EYGGVAGIVTLEDILEEIVGEIRDEFDEDEIPEIQTISKNTWEVDSRLSISDFNEHTG
                     CAIPDQEFDTIGGFVFDLFGKIPKKDEQIKYNNVTFKIKDIQGTRIGRIIVTVTPQKN
                     SDTY"
     gene            1360..2103
                     /gene="recO"
                     /locus_tag="PK348_02425"
     CDS             1360..2103
                     /gene="recO"
                     /locus_tag="PK348_02425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HXK65100.1"
                     /translation="MEIIKSEGIILKKIPVLEADVADIIYTQNHGKRTFIFKGIKKTK
                     RRSQAAIEPGSVVHLVYYYNEQKAMYHVKEFSLLLDTVSIRDNYQAMITLFLLLEVVE
                     KTTGHNDPNITIYKLLKGAIETLHKTSAYTFLSLSFILHYLKLSGIIPDYLHCSVCHK
                     EITDDLYLTEYQLISYCNQCAPNNAFVFKATAPLIYEILTKKFLNIETDIYNSIDIQK
                     LLFHTLLFIDHYFNIHIKTKELLYNNTKL"
     gene            2205..2975
                     /locus_tag="PK348_02430"
     CDS             2205..2975
                     /locus_tag="PK348_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65101.1"
                     /translation="MFKRFFIIVAIMAVSIFLIQPGYACFDTFLFLHHKSMVYPTGFF
                     AADVLGEYSFNDTDAPDQDSYFTNYSMYYGLAERVSVQFGLTQAETTRAENKYSLESW
                     SVRGVFNTIRSNSGNYFMDLVAEHHTGVDIDENMAHFSIPNLLYLSNFIVVIHPVYEL
                     TYSKNDREHTFGGHGGLFYNINNIGIIGVGGEFASAQSSGALGKRFTEGEAAASLFIG
                     FNLGNVYFQNEFAKGLNNSRDFGFAATLKFFFNTGMSI"
     gene            complement(3235..4185)
                     /locus_tag="PK348_02435"
     CDS             complement(3235..4185)
                     /locus_tag="PK348_02435"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HXK65102.1"
                     /translation="MARILVIAEQRNGKISDATLELCKAAKALASAMGASPAAAVFYK
                     DDSLAKEVAQYIPEVFAVVDPKLEVYSADTYVAAAKAVVEAQDVKGVLVPHTYDGTDY
                     AAKLALNLGCGIVTNCNKFEVQGGAVVFTRNTYNGKIQEQRSVKTDKFVATFEKGAFE
                     KEAAGGAGSVTAVSASIPEPRRKFKQFVETMAGSVDISQAKIIVSGGRGTKEKDKYND
                     IIVNFAKKLGGEYAASRPVVDAGWTDAARQVGQSGKTVTPDLYIACGISGAIQHVAGM
                     KGSKVIVAINKDPEAPIFNIATYGIVGDLFEVIPAIMEQL"
     gene            complement(4197..4985)
                     /locus_tag="PK348_02440"
     CDS             complement(4197..4985)
                     /locus_tag="PK348_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HXK65103.1"
                     /translation="MKIAVLVKQVPDMESKFKIIGDSKIDESQIAFKMNDFDEYAVEA
                     ALQLKEKFGGEVIIISAGPERASKEIRQAFAMGGDWGIQIQDEQVDAGDNFVVASALC
                     KAVESLGGVDLVLTGVQAEDDQAAVTGVMIADMLGLPHCTNVVKIEVGGDGKSMTVNR
                     ELEGGLNEVLEMAMPAVLTIQSGINQPRYPTLPGIMKAKKKRLDVKKAADLGVSAVGD
                     AGSKTKFVKMFFPVSDHKAEIIEGDPKTAAAKLVEKLRNEAKVI"
     gene            complement(5115..>5508)
                     /locus_tag="PK348_02445"
     CDS             complement(5115..>5508)
                     /locus_tag="PK348_02445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65104.1"
                     /translation="LMDVKTGVVIAHYNESDNTKYSLQTVSLDIAKNLYSTIPYWGKI
                     QSYSDDMVSVNLGSIDGIEIDSMLETFVDNEDTIDSEKPRKKVILQVKEVDTRVLLAK
                     PINPKDIDLLSKGQEVYPVSKRRAKKIN"
BASE COUNT         1767 a         1014 c          904 g         1823 t
ORIGIN      
        1 aaccaccagt taagaatgca aaaggtacaa atggattctg gatcccataa tacgcaagaa
       61 tagacaataa aattaccagg atcacgaata ccaaagagac caccttgtac tgttgcttaa
      121 gataagcaaa agccccttcg cgtacagcct gcgcaatctc gaccattgtt tcatttcctt
      181 tatctgcttt catcaccatt ttataaaaga tgaaggcaaa taaaagggca atgatcgacc
      241 cgacaggggc aatccaaaaa agcatactat ccatagggcc actccttttt ataaatttta
      301 attcagaatg ttaaaacaat taaaataata aagtaatctt ttgatgtcta aaaaaaagaa
      361 ataccgttta ttgtcaagtg tattttaaaa ttaaaaatgc cacttatctt ctggtaataa
      421 acacctggta aaaatcaaat tgacaatttg ccagtcacat tggtactatg ctgaattata
      481 agaaaaggag gtaaaataat tatatttact atggcttcgg atgagcaaaa gcccaaaaaa
      541 actaataata atgatgcagt atctttatta aaacaaaaat tctttaaatt tatacataaa
      601 aaaaatatat ctgttccagt tgataagtta gccatccaga aacaggagat gattcagggc
      661 attttaagcc tttccaccaa aaatgtaagg gaagtaatga taccccgagt tgatatcaat
      721 gctgttgagg atactatcca gttgaaggaa ctggtaaaac gtgttattga cgaaggtcat
      781 tcgcgtatac ctgtatattc aggaactatc gacaatatta ctggcatttt gtatgttaaa
      841 gaccttatgc catttattat cgacaggcga atgaaattca atataaagaa gcttttgcac
      901 aagccatatt ttgtacctga aacaatgact ttagatgaac tgctgctaca gtttcagaag
      961 caaaatttac atctggcgat agttgttgat gaatatggtg gggttgctgg cattgtaaca
     1021 cttgaagata tccttgaaga aatcgttggt gaaattcggg atgaatttga tgaagatgaa
     1081 attcctgaaa tacagacaat ttcaaaaaat acatgggagg ttgattcccg gttatcaata
     1141 tcagatttta atgaacatac tggttgcgct atacctgatc aggaatttga tacaattgga
     1201 ggatttgttt ttgacctttt tggaaagatt ccaaagaaag acgaacaaat aaaatacaat
     1261 aatgtaacat tcaaaattaa agatatacag ggtactcgca ttggcaggat tattgtaaca
     1321 gttaccccac aaaaaaattc agatacctat taaaattcaa tggaaatcat taaatctgaa
     1381 ggaataatat taaaaaaaat tcctgtcctg gaagcagatg ttgcagacat tatctatacc
     1441 cagaaccatg gtaaacggac atttatattt aagggaatta aaaaaacaaa acggcgcagc
     1501 caggctgcaa tagagcctgg tagtgttgta caccttgtat actattacaa tgaacaaaaa
     1561 gcaatgtatc atgtaaagga attttcgctt ttactggata cggtatcaat tagagataat
     1621 tatcaggcaa tgattaccct ttttttacta ctggaggttg ttgaaaaaac cactggtcac
     1681 aatgacccga atattacaat atataagcta ttaaaaggag ctatcgaaac attacataaa
     1741 accagtgcat atacttttct ttctctttct tttattcttc attatcttaa attatcaggg
     1801 attatacctg attacttaca ttgttcagtt tgccataaag aaattactga tgacctgtat
     1861 ttaactgaat accaacttat ttcgtattgt aaccaatgtg cacctaacaa tgcatttgtt
     1921 tttaaggcaa cagctccatt aatttatgaa attctgacta aaaaatttct aaatattgaa
     1981 actgatatat ataattcaat agatatacaa aagcttcttt tccatacatt attgtttatt
     2041 gatcattatt ttaatataca tataaaaact aaggaattgc tatataataa cactaagtta
     2101 taattaatat tatcttataa atatataaaa atattattga cattaagtgc tattaatatg
     2161 attttagtat tcatttaata ctaattttta aaggagggtg tattatgttt aagcggtttt
     2221 ttattatagt tgctattatg gcagtatcta tttttttgat acagccagga tatgcatgtt
     2281 ttgatacttt tttattctta catcataaat ctatggtgta tccaactggt ttttttgcag
     2341 cagatgtatt gggtgaatat tcattcaacg atactgatgc tcccgatcag gattcatatt
     2401 ttaccaatta cagtatgtat tatggattgg ccgaaagagt ttcagttcag tttggcttaa
     2461 ctcaggcaga aaccacacgt gcagaaaaca aatattcatt agaatcatgg tcagtaagag
     2521 gtgtatttaa tacaatccgt tcaaatagtg gaaattattt tatggatttg gttgcagagc
     2581 atcatacagg tgtagatatt gatgaaaata tggcacattt ttctatacca aatcttttgt
     2641 atctgtcaaa tttcattgtt gttattcatc cagtatatga attaacctat agtaaaaatg
     2701 acagagagca tacatttggt ggacatggtg gtttgtttta taatataaac aatataggta
     2761 ttataggtgt tggtggagaa tttgcatctg ctcaatccag cggtgcattg ggtaaacgtt
     2821 ttactgaagg ggaagctgca gcaagccttt ttattggatt taatctgggc aatgtatatt
     2881 ttcaaaatga atttgcaaaa ggattaaata attcaaggga ttttggtttt gcagcaactt
     2941 taaaattttt ctttaacact ggtatgagca tataaattaa aaaaggccgt tgcatatcaa
     3001 accatgtgac ggccttgttt tataaataat taaacttcac aaacgtttca atctccttat
     3061 tatcaagttg tttgtaattt ttcttcctgc tgaattcagt atccactatg attctttttg
     3121 atagtatttt taaatttttt cagattatca atatctagta tatggtaata aattaattaa
     3181 aaaaagaggt gtcttttggg agacacccct tttttaatac gattttactg ttaactatag
     3241 ctgttccata atcgcaggta ttacttcaaa aaggtcaccc acgataccat aggtagcaat
     3301 gttaaaaata ggtgcttcag ggtctttgtt gatagcaact ataactttag aacctttcat
     3361 accagctacg tgctggatag cacctgaaat accacaagct atataaagat ctggagtaac
     3421 agtttttcct gactgtccta cctgacgtgc agcgtctgtc caaccagcat caacaacagg
     3481 gcgagatgca gcatattcac cgccgagctt cttggcaaaa tttacgatga tatcgttata
     3541 tttatccttt tcctttgttc cacgtccacc ggatactatg atcttagcct gtgaaatgtc
     3601 aactgagcca gccatagttt ccacaaactg tttaaatttt cttcgtggtt caggaattga
     3661 agctgatact gcagttactg aacctgcacc acctgcagct tctttttcaa aagcaccttt
     3721 ttcaaatgtt gcaacaaact tatcggtttt aactgatctc tgttcctgta ttttgccatt
     3781 ataggtgtta cgtgtaaata caacggcacc accctgcact tcaaatttgt tgcagtttgt
     3841 aactatacca catcctaaat tcaatgccaa tttagcggca taatctgtac catcgtatgt
     3901 atggggtact aacacaccct ttacatcctg tgcttctacc actgccttag cagcagcaac
     3961 atacgtatca gcactataaa cttcaagttt aggatctact actgcaaata cttctggaat
     4021 atactgtgca acttcctttg ctaaactgtc atctttatag aatactgcag cagcagggga
     4081 tgcacccatt gcagatgcta atgcctttgc agctttgcaa agttctaatg tagcatcgct
     4141 tatttttccg tttctctgtt ctgctattac taatatacgt gccattgtgt acccccttat
     4201 atcacttttg cttcattgcg tagtttttct acaagcttgg cagcagcggt ttttggatca
     4261 ccttcaataa tttctgcctt gtgatctgat accgggaaga acatcttaac aaattttgtc
     4321 tttgaaccag catcaccaac agcagatact ccaagatctg cagccttctt gacatcaagt
     4381 cttttcttct tagctttcat aataccagga agggtcggat accgtggctg attaatacct
     4441 gactgaattg taagaactgc aggcatagcc atctcaagta cttcatttaa tccaccttca
     4501 agctcacggt taacggtcat tgatttgcca tcgccaccaa cttcaatttt aacaacattt
     4561 gtacaatgtg gcaagccaag catatcagca atcataacac cagtgactgc tgcctggtca
     4621 tcttcggcct gaacaccggt cagtacaagg tcaacaccgc ccaagctttc aacagctttg
     4681 cacagtgctg atgcaaccac aaaattgtca cctgcatcaa cctgctcatc ctgaatctgg
     4741 ataccccagt cgccacccat tgcaaatgcc tgacgaattt cttttgatgc tcgctcaggg
     4801 cctgctgaaa tgataattac ttcaccacca aatttttctt tcaattgcag cgcagcctct
     4861 acagcatatt catcaaaatc attcatcttg aaagcaatct gggattcgtc gatcttactg
     4921 tcgccaataa ttttgaattt ggattccata tctggaacct gcttaactaa gacagctatc
     4981 ttcatagtcc cactcccttt tataaaattt tatgcgaaaa aattagaata agcccatttg
     5041 tgcacaatgg tataaattgc attttatatg tcaagattta ttagtatatt tcattatata
     5101 aatttatata ctgactaatt tatttttttt gctcgtcgtt ttgaaacagg atacacttcc
     5161 tgtccttttg aaagcaggtc aatatcttta gggtttatag gtttagctaa aagaaccctg
     5221 gtatcaactt ccttcacctg taatattact ttttttcggg gtttttcgga atcaatggta
     5281 tcttcattat ctacaaatgt ttcaagcatg ctgtctattt caataccatc tatactcccc
     5341 aggtttactg acaccatatc atctgagtaa gactgaattt taccccagta cggaatggtg
     5401 ctatacaaat ttttggctat atccagagat accgtttgta atgaatattt ggtattgtcc
     5461 gattcgttat agtgagctat tactactcca gtttttacat ccatcaat
//
LOCUS       DAOSZP010000069         5493 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_12462_length_5493_cov_2.897389, whole genome shotgun sequence.
ACCESSION   DAOSZP010000069 DAOSZP010000000
VERSION     DAOSZP010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5493
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12462_length_5493_cov_2.897389"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(87..869)
                     /locus_tag="PK348_02450"
     CDS             complement(87..869)
                     /locus_tag="PK348_02450"
                     /inference="COORDINATES: protein motif:HMM:NF005596.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HXK65105.1"
                     /translation="MIDYHVHTSLCDHATGTPQEYIEKAIEAELFEIGFSDHAPLPLH
                     LRQNITMEPEETEAYVAMLQELTDKYPVAVKIGFEVDYPLHPTFDTRYLNDRRLDYII
                     GSCHFIGDWPFDHPAFVDEYTKRDINQVYRDYYHIVYDMVATGMFDIIGHFDLVKKFG
                     YRATADLNTEIEPILQLAAQQSTAIEINTSGWRKPVKEAYPSFEIIQKMFQYNVPITL
                     GSDAHAPEEVAYNFYEALSLIKQAGYRKIVGYRKRKQYTITF"
     gene            complement(875..1528)
                     /locus_tag="PK348_02455"
     CDS             complement(875..1528)
                     /locus_tag="PK348_02455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65106.1"
                     /translation="MFDIFESHEYRPPVFENMQMEISDIDNDISSDIDNLLANRFDSL
                     YMKDVYKKQHVAVEIIYPRIQYNHHKVLLKSPDYFRFLLSLYPFVSDLENVAKIVIRP
                     RHVVLGDVELMGLYLHRKQILVIYLHQPHFYSLKTARFARFAEFTPLAHLLQSKVISA
                     QNSENDPYLIPPLWYMLSLIEASHSDKVDKFLLRCDTTSKLQAALNEISFFYGRHGY"
     gene            complement(1537..2523)
                     /locus_tag="PK348_02460"
     CDS             complement(1537..2523)
                     /locus_tag="PK348_02460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65107.1"
                     /translation="MGIQDIIIFTKDEEELVETLKHAIKDKYTSEYTLIEKHKEALRS
                     LATSISLYPSLLDSQRLSNQKRTMESLLDTLCSRSIPDMILHIPTKAILGRAFTIAKI
                     NFFIMLWYIVRDRDEYASFLDTLLSCIASNVFMLTAEEVYTSIIEDDTLALNIRHNAA
                     YLLARTWEHRLDYGVAEFAPILLNLWKARERLIPNFGTMMGFSELCMLSEHTSPLWLD
                     FLQRDTITEDEVYAVEEFIFDLSFEEIVFVRDYLEKHNKITVSRDELPSILQKTHIPE
                     YQGQDPRELYRSFRDRKINSRFRARSGLQGPKKTLEEYLMCFLLSSRSMVEY"
     gene            complement(2806..4176)
                     /locus_tag="PK348_02465"
     CDS             complement(2806..4176)
                     /locus_tag="PK348_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007090220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HXK65108.1"
                     /translation="MANREHWGSKMGFVLAAAGSAIGLGNVWKFPYVTGSNGGAAFVF
                     IYLISVVILGLPIMIAELVIGRHTEKDPVGAFKQIAGGTTWEFVGYLGVLSGFFILSF
                     YSVVGGWTIGYIVKGITGSIAGLHDTAQASEIFKQYVSNPYLSVLYHVLFMLSCMYIV
                     IKGIKNGIEKWSKILMPLLFVILIALIVRGITMEGSLEGIIFFLNPDFSKITLNSILS
                     ALGQSFFSLSLGMGAMITYGSYLSREDKILHSALWIVFLDTLIAILAGLAIFPSVFAM
                     SLSPQEGPGLIFHVIPAVFARMPLGQFFEVLFFVLLFIAALTSGISLLEVVVAYITDE
                     KGWSRRKAVLVMGTIIIILGIPSALSFGVMGNMTILGNTFFDFMDKLTSNYMLPIGGF
                     FIAICLGWKYGLENTIHELDPDTRFISLKELWAFMIKFVSPFILFVVFFMLIKDDIVA
                     LLQKVF"
     gene            4452..5273
                     /gene="ccsA"
                     /locus_tag="PK348_02470"
     CDS             4452..5273
                     /gene="ccsA"
                     /locus_tag="PK348_02470"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HXK65109.1"
                     /translation="MILIYIITLLFIVASAYSILSIHKDMPRYVTATKLLMSIILIVL
                     LVIGYCMPVYIDSVYKIHIFTFFYLIMLFLTLLTLYMAISQWNNDYRSLSAILIPLTT
                     IISIISLFFINSPRHMTIGPDNSIMVIHIVLALLGEVLFFVAFASSVLYIIVSWQLQK
                     KSSMKFINRFPSLTVLSKLTSFSLSRSLFFFTAGIVIGIIMLFQFYGNISSGSPKEIL
                     MYVAWGIIFILWLISRSHITSNYYLSIMTIVSFLLLMILFIASNVVITSGFHSFR"
     gene            5280..>5493
                     /locus_tag="PK348_02475"
     CDS             5280..>5493
                     /locus_tag="PK348_02475"
                     /inference="COORDINATES: protein motif:HMM:NF017051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HXK65110.1"
                     /translation="MKEHIPQIGMVGMSHKTAPVETRECFAFDTQVLESFVNNAKKHS
                     IQEMVYIATCNRMELYFVSNQIEEATQ"
BASE COUNT         1766 a         1102 c          911 g         1714 t
ORIGIN      
        1 taatgccagc ctgcaatcca cataaaccat agattctgaa aatgtgttga gggtctttat
       61 ccagacgtat catatatgat tctttattaa aatgtgattg tatattgttt tctttttctg
      121 taaccaacta tttttctgta ccccgcctgt tttataaggc tcaatgcttc ataaaaatta
      181 tatgccactt cttccggagc atgggcatct gaacccagtg taattggaac attatactga
      241 aacatttttt gtatgatttc aaatgatggg tatgcctctt tgacaggctt tcgccatcct
      301 gacgtattta tctcaattgc agtagattgc tgtgcagcta attgtaatat tggttcaatt
      361 tctgtgttta aatcagcagt tgccctgtaa ccaaattttt taaccaggtc aaaatgccca
      421 ataatatcaa acatacctgt tgccaccata tcatacacta tatggtaata atcacgatat
      481 acctgattga tatcccgttt agtatattca tcaacaaatg ccggatggtc aaatggccaa
      541 tcgccaataa aatggcatga accaattata taatctaaac gcctgtcatt taaatatcga
      601 gtgtcaaacg ttgggtgcag cggataatcc acctcaaaac ctatctttac agccactgga
      661 tatttatcag ttaattcctg caacatggca acatatgctt cagtttcctc tggctccata
      721 gtaatgtttt gccttaagtg caatggcaaa ggtgcatggt cagaaaaacc tatctcaaat
      781 aattctgctt ctattgcttt ttcaatatat tcctgcggag ttcctgttgc atggtcgcac
      841 agggaggtat gtacatggta atctatcatg taaattaata tccatgacgt ccataaaaga
      901 aagatatctc attcagtgct gcctggagtt tggaagtagt atcacaccgc aataaaaact
      961 tgtcaacttt gtcggaatga gatgcttcaa taagtgaaag catgtaccac agtggaggga
     1021 taagatatgg atcgttttca gaattttgtg cactgataac ttttgattgc aacaaatgtg
     1081 ccagcggggt aaactcagca aaccgggcaa agcgtgctgt ttttaatgag taaaaatgtg
     1141 gctggtgcag atagataaca agaatttgtt ttctgtgcag atacaatccc attagctcta
     1201 catcacccag aacaacatga cgtggacgga ttactatttt ggcaacattt tcaagatcac
     1261 tgacaaacgg atacagtgat agtaagaacc tgaagtaatc aggtgatttt aataatactt
     1321 tatggtgatt gtattggatg cggggataga taatttcaac agccacatgt tgttttttat
     1381 acacatcttt catatacaat gaatcaaacc tgtttgccag cagattatca atatcagagg
     1441 atatatcgtt atcaatatcg ctgatctcca tctgcatatt ttcaaatact ggcggcctgt
     1501 attcatgtga ttcaaaaata tcaaacatgg catctcctaa tattcaacca tactgcgaga
     1561 agataataaa aaacacatca gatattcttc aagcgttttt ttggggccct gcagccccga
     1621 acgagccctg aagcgcgagt taatctttcg gtccctgaat gatcggtata attctcgcgg
     1681 gtcctgaccc tgatattcag ggatatgagt tttttgcaaa attgacggca actcatccct
     1741 gcttaccgtg attttattat gcttttctaa ataatccctt acaaaaacta tctcttcaaa
     1801 acttaaatca aatataaact cctcaaccgc atatacctca tcttcggtaa ttgtgtcgcg
     1861 ctgtaaaaaa tcaagccata atggcgatgt atgttccgat agcatgcata actcagaaaa
     1921 acccatcatg gtgccaaagt tagggattag gcgttcgcgg gctttccaca aattaagcag
     1981 tattggggca aactcagcaa ctccataatc aaggcggtgt tcccaggttc ttgccagcaa
     2041 atatgcagca ttatgacgta tattcaatgc aagcgtgtca tcttctataa ttgatgtata
     2101 tacctcttcg gcagtgagca taaatacatt tgatgcaata catgagagca atgtatcaag
     2161 aaatgatgca tattcatctc tatcccttac aatataccac aacattatga aaaaattaat
     2221 ctttgctatg gtaaatgccc gtcctaaaat tgcctttgta gggatatgca aaatcatatc
     2281 aggaattgac ctggaacata aggtatccag caaactctcc attgtacgct tttgattact
     2341 caacctctgt gaatctaata gcgaggggta taacgaaatt gatgttgcta aactccgtaa
     2401 tgcttcctta tgtttttcta tcaaagtata ttctgacgta tacttatctt taatagcatg
     2461 ttttaatgtt tcaacaagct cttcttcatc tttagtaaat atgataatat cctgtatacc
     2521 cattgaatca accctcacct ataaataaat ccagtttgag aattttgtca atattaaatt
     2581 cgtataacaa attaataaaa aaagacgatc ccttacggaa ccgtcaatat cattagcgga
     2641 ttatgttgga aggtaactac tcaatgagta gcttcaccat taattgtaat aaaaacccct
     2701 ataatagtca aataaaattt ttctttttat tgataaatat ttcattgcat atcatgccgt
     2761 tttcagtaac gggtatatgc catatacatt atgtttacat gaaaactaaa ataccttttg
     2821 taacaatgca acaatatcat cctttatgag catgaaaaat actacaaata atataaaagg
     2881 cgacacaaac ttgatcatga atgcccacaa ctctttcaat gatataaacc gtgtatccgg
     2941 gtcaagctca tgaatggtat tttcaaggcc atacttccac cccaaacaga ttgcaataaa
     3001 gaaaccacct attggcaaca tgtaatttga ggtaagctta tccatgaaat caaaaaatgt
     3061 atttcccaat attgtcatat tccccattac cccaaatgaa agtgctgaag gtattcccag
     3121 aatgataata atggttccca ttacaagtac cgcctttctt ctactccacc ccttttcatc
     3181 ggttatgtat gccacaacca cttccagcag cgaaataccc gatgtcagtg ctgctataaa
     3241 caacaacaca aaaaacaaaa cttcaaaaaa ctgccccaac ggcatacgtg caaaaaccgc
     3301 tggtatgaca tggaaaatga gccccgggcc ttcctggggc gatagcgaca tcgcaaatac
     3361 tgatgggaaa atagcaaggc cagctaaaat tgcaatgagc gtatccagaa agactatcca
     3421 cagtgctgaa tgcaggattt tatcttcccg tgagagatag ctcccatagg ttatcattgc
     3481 ccccatacca aggcttaagc taaagaatga ttgccccaaa gctgaaagaa ttgaatttaa
     3541 cgttattttt gaaaaatcag gatttaaaaa gaaaattata ccttcaagac ttccttccat
     3601 tgttatgcca cgcacaatca gcgctatcaa aataacaaac aaaagtggca tcaatatttt
     3661 actccatttt tcaatgccat tttttatccc ttttatcact atgtacatac atgacagcat
     3721 aaagagaaca tggtataaca ctgatagata gggattggaa acatactgtt taaatatttc
     3781 agaagcctga gccgtatcgt gtaaacctgc tattgaaccg gtaatacctt taacaatata
     3841 accgatagtc cacccaccta ccacactata aaaggaaagg atgaaaaaac ctgatagcac
     3901 gcctaaatag ccaacaaatt cccaggtagt gccaccagct atctgtttaa atgcacccac
     3961 agggtctttt tcagtatggc ggccaataac cagttctgct atcataatgg gcagccccag
     4021 tataactact gaaataagat atataaatac aaatgctgca ccaccgttag agccggttac
     4081 atacggaaat ttccacacat tacccagccc tattgccgaa cctgctgcag ccagcacaaa
     4141 acccattttt gatccccaat gttcacggtt agccattctc attcacctct ttaaagtaaa
     4201 tagaattttt gcaaagctta cgtttttcct gaaaaaattg cgggtacaca cgttacaaaa
     4261 tagttgaata tacaagaatt attgcaatat tttttttatt aatcaaattt ttgccatgaa
     4321 ctatcgctta tattatttta aactacgttg atttacaaga atcaaccaat agtacttgac
     4381 acagtgtcaa atgaacaata attatgaatt gtattaaaat agatatatat taataaacaa
     4441 tccgaggatt tatgatttta atatatataa taacattact gtttattgtg gcatcggcat
     4501 acagcatcct ttccatacat aaggacatgc cacgctatgt gacagccact aaattactaa
     4561 tgagcattat tttgattgtg cttctggtaa ttgggtattg tatgcctgtg tatattgata
     4621 gtgtatataa aatacatatt tttactttct tttaccttat aatgcttttt ttaacattgc
     4681 ttactcttta tatggcaata tcacaatgga acaatgacta caggtcgttg agtgcaatac
     4741 ttattccact cacgaccatt ataagcatta tttcattgtt tttcataaac tccccgcgac
     4801 atatgaccat tggtcctgat aacagtatca tggtcatcca tattgtactg gcactgctgg
     4861 gtgaggtact gttctttgta gcttttgctt cttcggtgct ctatattatt gtttcatggc
     4921 aactgcagaa aaaatcatcc atgaaattta ttaaccgttt cccaagccta accgtattga
     4981 gtaaactcac cagcttctca ctgtcacgct cacttttctt ctttactgct ggtatcgtca
     5041 ttggcatcat catgttattt cagttttatg ggaatatatc atctggaagt cctaaagaaa
     5101 ttttaatgta tgtggcatgg ggtattattt ttattttatg gcttataagc aggtcacata
     5161 taaccagtaa ctactatctc agtattatga ctatagtatc ttttttattg cttatgattc
     5221 tgtttatagc cagcaatgtt gtgattacat caggattcca cagtttcagg taattaataa
     5281 tgaaagaaca tataccacag attggcatgg ttgggatgag tcataaaaca gctcccgttg
     5341 aaacacgtga gtgctttgcc tttgatacgc aggttctgga atcatttgta aataatgcaa
     5401 aaaagcattc catacaggaa atggtgtata ttgcaacctg caacaggatg gaactgtact
     5461 ttgtgtctaa tcaaatagaa gaagcaacgc aag
//
LOCUS       DAOSZP010000070         5487 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_12478_length_5487_cov_2.983800, whole genome shotgun sequence.
ACCESSION   DAOSZP010000070 DAOSZP010000000
VERSION     DAOSZP010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5487
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12478_length_5487_cov_2.983800"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..939
                     /locus_tag="PK348_02480"
     CDS             <1..939
                     /locus_tag="PK348_02480"
                     /inference="COORDINATES: protein motif:HMM:NF014820.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HXK65111.1"
                     /translation="GAVMRPVQTVTADFIQALGIGCMMINTGRFRTIAVEAHSKASNV
                     KTLNEVKAFALDPVYIRPLKENADFLAGLEMKRFLEETGNTEEQCAEVIVKNKANALN
                     NPYAAHAASLTIDDVLSSPPISLPLKELDAAKPADGAIMIVLASEKEAKALCKKPIWI
                     RGISWFSGQGNPWCRDFAYADYAQLAAQKAFAMAGIANPLKDLDFAEICDEYSYKELQ
                     HLESIGIARKGEAGFLTQTGVTKLGGLLPVNVSGGSLGVGHTYEASGGMKVVEAVMQL
                     RHEAGIRQIPNAKTGLVQSWRGVPTATGAIALLSNE"
     gene            955..2118
                     /locus_tag="PK348_02485"
     CDS             955..2118
                     /locus_tag="PK348_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013335676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase domain-containing protein"
                     /protein_id="HXK65112.1"
                     /translation="MGFRKVAIVGAGITKFVRRAQETGPEMSYLASKIALDQAGLKLK
                     DVEMVTLGTAPDAFDGVHMKGEYLLSGAGGNNKPYIRNFVGGGTGVFAPIHGWMHVAS
                     GACDVCLVVCSEKMSSCHPHPQGAFLTIFDHTTEQPLKPTLIWIFALEMRRYMETYGL
                     TKEDIARVAVKNKNNALDHPSAQVAEAVTIEDILNSEVLAEPVHRLDISPASDGAAAV
                     VLASEDVAKRLTDKPVWIDGVGWNLDTAYWATKDLYYPRYVEKAARMAYEMAGITEPH
                     KEIQVAEPYDPFDYKELHHMEGLLLCERGEAARLTAEGYTARTGNLPTCPSGGALGVG
                     NPIAATGLMKVIEIFLQLREEAGKRQVPGKPRVGVAQAWGDLMQVGTVIVLRR"
     gene            2128..2631
                     /locus_tag="PK348_02490"
     CDS             2128..2631
                     /locus_tag="PK348_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012966990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HXK65113.1"
                     /translation="MKIENVPVKGLSSDEYEKTLKISWKPTLQYAWDNGIGIGKYLQS
                     LKEGKILAAYCEKCGRIMLPARAFCEMCFRPTDKYLPVKDTGTVNTFAICHVNWDASR
                     LKPDEPRYIPAVIEIDGASPGQGIMHLLGNVEPAKVRIGMKVKAVWKPADKREGSITD
                     ILFFEPY"
     gene            2645..3115
                     /locus_tag="PK348_02495"
     CDS             2645..3115
                     /locus_tag="PK348_02495"
                     /inference="COORDINATES: protein motif:HMM:NF013920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HXK65114.1"
                     /translation="MSFKETIQSNIQLSYFEGQIPVNYQYTLGVAGNKFFETLKQKDE
                     FIASVCSHCGVKTIYPLIYCEECFNPIDTYVSIGLEGELYSFTKCYRDYQGNTYDEPH
                     VIGLIRFDGIKGGIIHRLNINEKDLKLGIRVKAKLKPTNKRTGGLDDIVCFEKV"
     gene            3165..4322
                     /locus_tag="PK348_02500"
     CDS             3165..4322
                     /locus_tag="PK348_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014956194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXK65115.1"
                     /translation="MYDFGLTEEQKLLRQNVREFAEKEILPVAQELDETETFSVELTK
                     KMGSMGLFGIVVPQEYGGAGMDYLSYAIVVEEIARIDGSQAATVAAGNSLGIGPIYYF
                     GNEKQKQEWLPRLCKGEILASFGLTEPEAGSDAGASKTTARLEHGQWVINGSKIFITN
                     STSPMAGVCTVQAITGKRDNGKKEVSCILVPNGTPGFTAKKMTRKMMWRASDTGELYF
                     DDCKVPEENLLGKRGEGFHQMLSTLDNGRLAIAAMGLGGAQGAYELALKYAKQRKQFG
                     QPISTFQAIAFKLAQMATEIEAARNLLYKACKLKDAGLPFSKHAAMAKLYCSEVMGRV
                     VDQAVQIHGGYGLMKEYNVERFYRDYKLLTIGEGTSEIQKIVISRQIGCYE"
     gene            4595..>5487
                     /locus_tag="PK348_02505"
     CDS             4595..>5487
                     /locus_tag="PK348_02505"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HXK65116.1"
                     /translation="MKQKQKPHNFAIFVARQPIFNRHKELYGYEILFRSGFENFYNAL
                     DADASTSKTLINSFLVIGTDSLTGGRRAFINFTEETLKTNLAYFFPKEVLTIEILETV
                     PITHEIITIAKKLKSQGYKIALDDFVFTDEYRELLPFADIIKVDFLNTTTFERIQIHN
                     ETAHLNIKLLAEKIETYEDFHEALSLGYSYFQGFFFSQPEIIESRDIPVYKLNYLEIL
                     QQIHKEDVDFGKLEEIIKRDVSLSFKLLKFINSAAFGFASQIGSIKHALALLGIREFK
                     NWLSFVVLHSIAQDKPQELIVT"
BASE COUNT         1815 a          979 c         1181 g         1512 t
ORIGIN      
        1 ggtgcagtta tgcgtcctgt acaaacagtt actgcagatt ttattcaggc tttaggcata
       61 ggatgtatga tgatcaatac tggtagattc aggacaattg cagttgaagc acatagcaaa
      121 gcttcaaatg taaagacgtt gaatgaagtt aaagcatttg cccttgatcc tgtatatatc
      181 agacctctta aagaaaatgc tgacttttta gctggattag aaatgaagcg ttttcttgaa
      241 gaaacaggaa acaccgagga acaatgtgca gaggttatag taaaaaataa agctaatgct
      301 cttaacaatc cttatgcagc acatgctgca agcctaacaa ttgatgacgt tctatcatca
      361 ccacccatat cgcttcctct taaagaactg gatgctgcaa agcctgctga tggtgcaatt
      421 atgattgtac tggcatcaga aaaggaagca aaagcattgt gtaaaaaacc aatttggatt
      481 cgcggtataa gttggttttc agggcagggt aatccatggt gtcgagattt tgcttatgct
      541 gattatgcac aattagctgc acaaaaagct tttgcaatgg caggtatagc taatcctttg
      601 aaagacctgg attttgctga aatatgcgat gaatattcat ataaagaact acaacattta
      661 gaatcaattg gcattgcacg aaaaggtgaa gccggttttc taacacaaac aggtgttaca
      721 aaattaggag gactattacc cgttaatgtt tcagggggtt cattgggtgt tggccataca
      781 tacgaagcta gtggcggaat gaaagtcgtt gaagcagtta tgcaattgag gcatgaagcc
      841 ggaatccgcc aaatacctaa tgcaaagact ggtcttgttc agtcttggcg gggtgttcca
      901 accgccactg gagctatcgc cttattatca aatgaataaa ggaggagggt gtatatggga
      961 tttagaaaag ttgcaattgt cggcgcaggc ataactaagt ttgtacgcag agcacaggaa
     1021 accggccctg aaatgtcata cctagcttca aagatagcac tggatcaggc tggattaaaa
     1081 cttaaagatg tagaaatggt cacattaggc acagcacctg atgcgtttga tggggttcat
     1141 atgaaaggtg agtatctttt gagcggtgct ggtggcaata ataaaccata tatacgaaat
     1201 tttgtaggag gtggcacagg agtttttgca ccaattcacg gctggatgca tgttgcatct
     1261 ggtgcatgtg atgtatgcct ggttgtatgt tctgaaaaaa tgtcatcctg ccatccgcat
     1321 cctcaaggag catttttaac tatttttgac catacaaccg aacaaccgtt aaaaccaaca
     1381 ttgatttgga tctttgcatt agaaatgcgt cgttacatgg aaacatatgg attaaccaaa
     1441 gaagatattg ctcgtgttgc agtaaaaaat aaaaacaatg ctcttgacca tccatcagca
     1501 caggtagccg aagcagtaac cattgaagat attttaaatt ctgaagtttt agctgagccc
     1561 gttcaccgtt tagatattag cccggcatca gatggtgctg cagctgtagt tttggcaagc
     1621 gaagacgtag caaagcgatt gactgataaa ccggtatgga ttgatggcgt aggttggaat
     1681 ttagatacag catattgggc aacaaaagac ctttattatc ctcgatatgt tgaaaaagca
     1741 gcacgcatgg catacgagat ggcaggcatt acagagccac acaaagaaat acaggtagcg
     1801 gaaccctatg acccctttga ctataaagag cttcaccata tggaaggctt gttattgtgt
     1861 gagcgtggcg aagctgccag acttacagcc gaagggtata cagcacgaac aggaaatctt
     1921 cccacatgtc cttctggtgg cgcactggga gtaggcaatc caattgctgc aactggattg
     1981 atgaaggtca tcgagatttt cctacaattg cgtgaagaag caggcaaacg tcaggtccct
     2041 ggaaagccac gtgtaggagt tgcccaagca tggggcgatc ttatgcaggt tggcacagtt
     2101 atagtattac gacgatagga ggcaaatatg aaaatagaaa atgttccagt aaaaggtctt
     2161 tcaagtgatg aatatgaaaa aactcttaaa atatcatgga aaccaacttt acaatatgct
     2221 tgggataatg gaattggcat tggcaaatat ctgcagtcgt taaaagaagg aaaaatttta
     2281 gctgcatatt gtgaaaaatg cggccgcata atgttgcccg cccgtgcttt ttgcgaaatg
     2341 tgctttaggc caactgataa atatttacct gttaaagata ctggcactgt aaatacattt
     2401 gcaatatgcc atgtcaactg ggatgccagc cgtcttaaac ctgatgaacc gcgctacatc
     2461 cctgcagtta ttgagataga tggtgcttca ccaggtcagg gaattatgca cttacttggc
     2521 aatgttgaac cagcaaaagt gcgtattggt atgaaggtta aggcagtatg gaaacctgca
     2581 gataaaagag aaggttcaat aactgatatt cttttctttg aaccatacta aaaggaggaa
     2641 tgccatgtca tttaaagaaa ctattcaatc aaatatacaa ttaagttatt ttgaaggcca
     2701 gataccagtt aattatcagt atactctggg agtagctgga aataaatttt ttgaaacact
     2761 taaacaaaaa gacgaattta ttgcatcagt atgttcacat tgtggcgtaa aaactatata
     2821 cccattgatt tattgtgaag agtgctttaa tccaattgat acatatgtat caatcggatt
     2881 agagggcgaa ttgtattcat ttaccaaatg ttatagagat tatcagggta atacatacga
     2941 tgaaccacat gttataggct taattcgatt tgatggaatt aaaggcggaa taatacaccg
     3001 tttaaatatt aatgaaaaag atttaaaatt aggaatcagg gtaaaagcaa aattaaagcc
     3061 tacaaataaa cgcactggtg gtcttgatga tattgtgtgc tttgaaaaag tataaatacc
     3121 aaaacaacat aatgttacca taaaaataat aaaagaggtt ccccatgtat gattttgggt
     3181 taaccgagga acaaaagctt cttcgccaga atgtgcgaga atttgctgaa aaagaaattt
     3241 tacccgtggc tcaggaactt gatgaaacgg aaaccttttc agtagagctg acaaaaaaaa
     3301 tgggttccat gggccttttt gggattgtcg tgccacagga atatggtggt gcaggcatgg
     3361 attatttatc atatgcgata gttgttgaag aaatagctcg catcgatggt tcacaggctg
     3421 caacagtagc tgctggcaat tccttaggga ttggtcccat atattatttt ggcaatgaga
     3481 agcagaagca agagtggctg cctcgtctgt gcaaaggcga aatactggca tcatttggtc
     3541 ttactgaacc ggaagccggt tctgacgcag gagcaagcaa aaccactgcc cgtttagaac
     3601 atgggcagtg ggttataaac ggcagcaaaa tatttatcac aaactcaaca tcaccaatgg
     3661 caggcgtgtg cacagttcag gcaatcactg gcaaacgaga taatgggaaa aaagaagtat
     3721 cgtgtatact ggtgccaaat ggcactccag gttttacagc aaaaaagatg acccgaaaaa
     3781 tgatgtggcg tgcatctgac acaggggaac tttactttga tgactgcaag gtgccggagg
     3841 aaaatctact gggcaaacgt ggagaaggat ttcatcagat gctatcgaca cttgataatg
     3901 gaaggcttgc aatagccgcc atgggccttg gtggtgcaca gggagcttac gagttagcat
     3961 taaaatatgc taaacaacgc aaacaatttg ggcaaccaat ttcaaccttc caggcaatag
     4021 catttaagct tgcccagatg gcaactgaaa ttgaagccgc acgaaactta ttgtataaag
     4081 cctgcaagtt aaaagacgct ggcctcccct tctcaaagca tgccgccatg gcaaaactat
     4141 actgctctga ggtcatgggg cgggtggtag accaggcagt gcaaattcat ggtgggtatg
     4201 gacttatgaa agaatataat gttgagcgct tctacaggga ttacaagctg ttaaccattg
     4261 gtgaagggac atcagaaata cagaaaatag taatatcacg gcaaatcggc tgttatgagt
     4321 aaataaggca ccaacctttt atgccaatca tggaatttgt ttgaaaaacc tgagatcctg
     4381 aatccttatc cagggaaacc gtgtaaaaat tcttgacaag aaaaatatcc ataaaaattt
     4441 tttcctttat ataaaatact tgccaataat agtgttgtaa tatatcatta atagtgtaaa
     4501 ttttttatta caaattttat actatttttg aattagattt atatatccct cttataaacg
     4561 attgggatat aaactttaaa tccaacttgt tattatgaaa caaaaacaaa agccgcataa
     4621 ttttgccatt ttcgttgcac ggcaaccaat atttaacagg cataaagaat tatatggcta
     4681 tgaaatatta ttccgtagcg gctttgaaaa tttttataat gcacttgatg ctgatgcatc
     4741 aacatcaaaa acccttatta atagcttttt agtcattgga accgattcac tgacaggtgg
     4801 cagaagagca ttcattaatt ttactgaaga aaccttaaaa acaaaccttg cctatttttt
     4861 cccaaaagaa gtcctgacaa ttgaaatact tgaaacagtt cccattacac atgaaatcat
     4921 cactattgca aaaaagctaa aaagccaggg gtataaaatc gcgcttgatg attttgtatt
     4981 tactgatgaa taccgtgaat tactcccttt tgcggatatc ataaaagttg attttttaaa
     5041 cacgactacc tttgagcgca ttcagataca taatgaaaca gcacatctca acatcaaact
     5101 actggctgaa aagattgaaa cctatgaaga ttttcacgaa gcactttcac tgggatactc
     5161 atactttcag ggatttttct tcagccagcc agagattata gaatcaagag atattcctgt
     5221 ctataaactc aactacttag aaatattaca gcaaatccat aaggaagatg ttgattttgg
     5281 caaattagaa gagataataa agcgtgatgt atcattaagc tttaaactgc ttaaattcat
     5341 caattctgct gcgtttggat ttgccagtca gattggttca attaaacacg cactggcact
     5401 actgggtata cgtgaattca aaaactggct atcatttgtg gtgcttcaca gcatagcaca
     5461 ggataaacca caggaactca ttgtaac
//
LOCUS       DAOSZP010000071         5443 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_12602_length_5443_cov_3.195991, whole genome shotgun sequence.
ACCESSION   DAOSZP010000071 DAOSZP010000000
VERSION     DAOSZP010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5443)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5443)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5443
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12602_length_5443_cov_3.195991"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1275)
                     /gene="flhF"
                     /locus_tag="PK348_02510"
     CDS             complement(<1..1275)
                     /gene="flhF"
                     /locus_tag="PK348_02510"
                     /inference="COORDINATES: protein motif:HMM:TIGR03499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HXK65117.1"
                     /translation="MRYVKIKARTYNEAMMKLKMEHGDDAIPISHKNVKEGGLFNTGL
                     FARDFVELTAAIPDVKPAKLKPKKNIDLTVGTNDNVNELLKQVIEKPETEPHKKPHTG
                     LEQLLTQSILQSRTQQEEVKPVVKTVEEQKVETTDSSYIKLEKEFNELKALLKNLVET
                     KQERLSSDICLDNGDTYFKPFKEILRKNDMDDEECTMLLDEVKRNVSSEDMKDKYKIE
                     KTLRELIKSRIVVSGPIDRGKRKKVLMFVGPTGVGKTTTMAKLGAILSLRQGLKVSFI
                     TIDNYRIAATEQLKKYAEIMRIPIHVVTDPAEFKEIVENEKADIIMVDTSGRSHRNDL
                     KIAEIKNFADNCTCEIVKILCVSANTKRSDLSEVFKAYDILHIDNVIITKVDETSYIG
                     NVISVADKYNKPISYITNGQEVPNDIAIAEPDM"
     gene            complement(1312..3393)
                     /gene="flhA"
                     /locus_tag="PK348_02515"
     CDS             complement(1312..3393)
                     /gene="flhA"
                     /locus_tag="PK348_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004573406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HXK65118.1"
                     /translation="MAEQTYDFSWMQKSDILLGIGVIAVVTMLVIPLPTFLLDFLMAI
                     SIMLGILILLVVMYVPRSFDFSVFPALLLITTVYRLALNVSSTRLILLQGAAFDGKII
                     RTFGEFVVGGNYVIGFIVFVILVAVQFVVITKGATRTAEVAARFTLDAMPAKLMSIDS
                     DLSNGIINEEEALRRRREVRKEADFYGAMDGASKFVQGDVKVGIIITIVNIIGGFIIG
                     MVMRGESFDVAVHTYTLLSIGDGLVAQIPSLLITTATGIIVTRAVSEDSLGKDLSAQL
                     GSQPRALMLTAAALGLSAIIPGFPKITLILLALGIGALGYLLKQTEEEQIEKTKIEQK
                     EAALKTHKPESVIPLIQVDPLEVEIGYSLIPLADPDQGGTLLDRITNIRRRSALEMGL
                     IVPPIRIRDNMELAPQDYSILIKGVEVGHGSLQVGKLMAMDPGTVEEKIEGVEFTEPV
                     FNLKAIWIDPDNRDIAERRGYTVVDCPTIIATHLTEIIRRHADEILGRQEVQQLIDTI
                     KNDYPAVVNELVGDKKLSIGEIQKVLQNLLRERVSIRNMVTILETLASYSDYTKDIGL
                     LTEYVRVALARQICREYADKNNTLSVITVDPEIETIIRSSIHEDPVEGRIVSLDPDTH
                     REVLESLLGSYSKARSAGFAPVYLVSPHIRSVLFTLLEREVPDPVVLSYNEIVPEIRV
                     NVIGTALLRAA"
     gene            complement(3409..4566)
                     /gene="flhB"
                     /locus_tag="PK348_02520"
     CDS             complement(3409..4566)
                     /gene="flhB"
                     /locus_tag="PK348_02520"
                     /inference="COORDINATES: protein motif:HMM:TIGR00328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HXK65119.1"
                     /translation="MKWYEDINLQILSLHTSSAIVFDLQLFAAEDEGRTEEPTEKKLR
                     EAREKGQVAKTEELPQALVVIFAMITIVVFCGFIFETIIRMMHYYLTSFSHFQLTERS
                     LFHEAIATGIESFKILLPIFVATVIAALLGNIAQVGFQISTHPLKFDLSKIKFDPATM
                     ARKIFFSRQVAMNLFKSIFKVVVIGFVCYLVISNDFDELIKTPDISVGLAFQNISIIA
                     LKIIIWSTVCLLVLSIPDYIFQKREYIESLKMTKQELKEEWKETVGDPHVRARLQEMQ
                     RELIMRNMIREVPKADVVVTNPTHFAIAMRYEPEAMYAPMVIAKGVDAMALRIRQIAL
                     DNNILVIENRPLAQELYQRVEVGDIIPEDLFYAVSLIYAEVYKKRNYKAAI"
     gene            complement(4563..5342)
                     /gene="fliR"
                     /locus_tag="PK348_02525"
     CDS             complement(4563..5342)
                     /gene="fliR"
                     /locus_tag="PK348_02525"
                     /inference="COORDINATES: protein motif:HMM:TIGR01400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HXK65120.1"
                     /translation="MEYFVYQFQAFFLVLVRMTAMMVIAPFFSSGAIPLLMRGALSFF
                     VSLVIFPVLSISNAALTGNMGVYYLMVIQEIIIGIYIGFLVSVVFSAFQLSGQYFAVQ
                     IGFGINEVIDPLAQISIPLVGQLKNIIGLLVFLVINGHHFMIKAIFHSYELAPAFALK
                     GESIQAFLKFLAHSFSGMFVIALKISLPVIAAVFLVSVSLGILAKAAPQMNIMMLGFP
                     FKIAVAFGMMILISPLIVRVMNVSLERTFQFIVKVIRYWPV"
BASE COUNT         1625 a         1218 c          930 g         1670 t
ORIGIN      
        1 catatcaggt tcagcaattg ctatatcatt tggaacttcc tgtccattag tgatatatga
       61 tatgggctta ttatatttat cggctactga gatgacattt ccaatatatg atgtttcatc
      121 aacttttgtt attattacat tgtcaatatg caggatgtca tatgctttga atacctcaga
      181 tagatcactg cgttttgtgt tagcactaac acaaagtatc tttacaatct cacaggtaca
      241 gttatcagca aagtttttta tctcggcaat tttaagatca ttcctgtggc ttcttcccga
      301 tgtatctacc attataatgt cagctttttc attttcaact atttctttaa attcagccgg
      361 atcagtaaca acatgaattg gtatgcgcat tatttcagca tattttttaa gctgctcagt
      421 tgcggcaatt cggtaattat caatagtaat aaatgatact ttcaaaccct gccgcaaaga
      481 taatatagca cccaattttg ccatcgttgt tgttttacca acgcccgttg gacctacaaa
      541 catcaacact ttcttccgtt tgccccggtc aataggtcca cttaccacta tcctgctttt
      601 aatgagttca cgcaacgttt tttcaatttt atatttatct ttcatatctt cactgcttac
      661 attgcgtttt acttcatcta aaagcatagt gcattcttca tcatccatat catttttacg
      721 caaaatttcc ttaaacggtt taaaatatgt atcaccgtta tcaagacata tatccgaaga
      781 cagtctttct tgctttgtct caacaagatt tttaagtaaa gcttttaatt cgttaaattc
      841 cttctccaat tttatgtatg aagaatcggt tgtttcaact ttctgttcct caaccgtctt
      901 cactacaggc ttaacctctt cctgctgggt tcttgattgc agtatactct gagttaaaag
      961 ttgctcaagt cctgtatgtg gttttttatg tggttcagtt tcaggttttt ctataacctg
     1021 ttttaaaagc tcatttacat tatcattggt tccaacggtt aaatcgatgt tcttctttgg
     1081 ttttaatttt gcaggcttga catctggtat tgctgcagtc aactcaacaa aatcgcgtgc
     1141 aaacaatcct gtattaaaca aaccgccttc tttaacattc ttatggctga taggtatagc
     1201 atcatcacca tgttccatct tgagtttcat cattgcttca ttataggtgc gtgctttaat
     1261 ttttacgtat ctcattgtta attacctcgc tttaacaaat attttattat attatgctgc
     1321 ccgcaataac gctgtaccta taacattcac tctgatctcg ggcacaattt cattatacga
     1381 aagaacaacg ggatcaggga cctccctttc cagcaatgta aacaagacac tacgaatatg
     1441 tggcgatacc agatacaccg gtgcaaatcc tgcacttcgt gccttggaat atgaacccag
     1501 caaactctcc agtacctctc tatgcgtatc tggatcaagg ctaacgattc ttccctcaac
     1561 tggatcctcg tgtattgaag atcgtattat tgtttctatc tccggatcta ctgtaattac
     1621 cgaaagcgta ttgtttttat cagcatattc cctgcatatc tgacgtgcca gtgcaacacg
     1681 tacatactca gtcagtaaac ctatatcttt agtataatct gaatagcttg ccagtgtttc
     1741 cagaatggta accatatttc taattgaaac acgttctcgc aggaggtttt gcaatacttt
     1801 ctgtatttca cctatgctta gctttttatc cccaacaagt tcattaacca cagcaggata
     1861 gtcattctta attgtatcaa taagctgctg cacttcctgc ctgcccagta tttcatccgc
     1921 atgcctgcgt ataatctcag tgagatgtgt tgcaataata gtgggacaat ccacaacagt
     1981 atatcctctg cgctcagcaa tatctctgtt atcaggatct atccatattg ctttgaggtt
     2041 gaataccggt tcggtgaatt caacaccttc aattttttcc tcaacagtac cgggatccat
     2101 tgccatgagt tttcctacct ggaggcttcc atgcccaact tcaacacctt ttattaaaat
     2161 ggagtaatcc tgaggtgcaa gttccatatt atcccggata cggattggtg gcactataag
     2221 tcccatttca agggcgctac gtctgcgtat attggtaatc cgatcaagta atgtgccacc
     2281 ctggtcagga tcagccaggg gtataaggct gtaaccaatt tctacttcaa gaggatcaac
     2341 ctgaatgagt ggtattaccg attcaggttt atgagttttc aacgcagctt ctttttgctc
     2401 tatcttggtt ttttcaattt gttcctcttc agtttgtttt aacagatacc ctaatgcccc
     2461 aatacccaaa gctagtagta taagagttat tttggggaaa cccggaatga ttgcagataa
     2521 tcccaatgct gcagcagtaa gcatcaatgc acgtggttga gaacccaatt gcgccgaaag
     2581 gtctttcccc aggctatctt ctgatacagc acgggtaaca atgataccag ttgctgttgt
     2641 aataagcaat gatggaattt gagcaaccag gccatcgcct atcgacaata atgtgtatgt
     2701 atgaactgct acatcaaaac tttcaccccg cattaccatc cctatgataa aaccgccaat
     2761 aatattgact attgtaataa taatgccaac cttaacatcg ccctgtacaa attttgaagc
     2821 accatccatg gctccgtaaa aatcagcttc tttacgaact tcacgcctgc gccgtaatgc
     2881 ttcctcttcg ttgatgatac cattggaaag gtctgaatct atactcatga gtttagccgg
     2941 catggcatcc aatgtaaaac gggctgctac ttcggctgtt cgtgttgcac ctttggtgat
     3001 gacaacaaac tgcactgcaa caaggattac aaatacaatg aatccaataa cataattgcc
     3061 cccaacaaca aactcgccaa atgtgcgtat tattttacca tcaaatgcgg caccctgcaa
     3121 aaggattaag cgggttgaag aaacattaag cgccagacga taaacagtgg ttataagcag
     3181 caaggcaggg aatacagaaa aatcaaaaga gcgtggtaca tacatgacta ccagcaatat
     3241 caggattccc agcataatgc tgattgccat aagaaaatcc agaaggaacg ttggcaacgg
     3301 tataaccagc atggttacaa cagcaataac accaataccc agtagaatat cgctcttttg
     3361 catccatgaa aaatcgtatg tttgttctgc catacgctac ctcaacgatt atattgctgc
     3421 tttatagttt cttttcttat acacttcagc ataaattaac gaaacagcat aaaataaatc
     3481 ctcaggaata atatccccaa cttccacacg ttgatataat tcctgtgcca gtggcctgtt
     3541 ttcaataacc agtatgttgt tatcaagagc tatctgccgt atccttaaag ccattgcatc
     3601 aacaccctta gcaatgacca ttggcgcata cattgcttct ggttcatatc gcatagctat
     3661 ggcaaaatgt gttgggtttg ttactaccac atctgctttg ggtacttcac gtatcatgtt
     3721 gcgcataata agctctcgct gcatttcctg taaccgtgcc cgtacatgcg ggtcaccaac
     3781 agtttccttc cattcttctt tcaattcctg cttggtcatt tttaatgatt caatatattc
     3841 acgtttctgg aaaatataat ccggtatgga aaggacaagc aaacatactg ttgaccatat
     3901 aattatcttc agggcaataa tagagatatt ttgaaatgct aaaccaacac ttatgtcagg
     3961 cgttttaatc aattcatcaa aatcgttgct gataactaaa taacatacaa agccaataac
     4021 aaccacttta aaaatagatt taaatagatt catggcaacc tggcgggaaa agaatatctt
     4081 gcgcgccatt gttgctgggt caaattttat ttttgaaagg tcaaacttca ggggatgtgt
     4141 tgatatctga aatcccacct gcgctatatt acccagcagt gctgcaatca cagttgctac
     4201 aaatattggc aatagtatct tgaaggattc aatgccggtt gctatcgcct catgaaataa
     4261 acttcgttca gttagctgaa aatgtgaaaa gctggtaaga taataatgca tcattctgat
     4321 aattgtttca aagataaaac cacaaaacac aacaatggta atcatggcaa aaataaccac
     4381 cagagcctga ggcaattctt cggttttggc tacctgcccc ttttcgcgtg cttcacgaag
     4441 cttcttctcg gtaggctctt cagtcctgcc ttcatcctcg gctgcaaaaa gctgaaggtc
     4501 aaaaactatc gcactactcg tatgtagcga tagtatctgc agatttatgt cttcatacca
     4561 tttcatactg gccagtacct gatcacctta actatgaact ggaatgtcct ttctaacgac
     4621 acattcataa ctctgactat cagcggcgaa ataagaatca tcataccaaa tgctaccgca
     4681 atcttgaacg ggaagcccag catcataata ttcatctgtg gtgctgcctt tgctaaaatc
     4741 cccagactaa ctgaaacaag aaataccgct gctatgacag gcagcgaaat tttaagggct
     4801 atcacaaaca tgccgctgaa tgaatgagcc aaaaatttta aaaaagcctg gatgctttcg
     4861 cctttaagcg caaatgctgg cgccaattca tatgagtgga aaattgcttt aatcatgaaa
     4921 tggtggccgt taattactaa gaatactagc aaaccaataa tattttttag ctgccccaca
     4981 agcggaattg aaatctgagc aagggggtct atgacttcat ttataccaaa gcctatctgc
     5041 acggcaaagt attgcccgga aagctgaaat gctgaaaata caacactaac aagaaaccca
     5101 atatatattc ctattattat ctcctgtata accataaggt agtacacacc catattgccc
     5161 gtcaacgcag cattgctgat tgaaagtaca gggaagataa ccagtgacac aaaaaacgaa
     5221 agcgcccccc tcataagtaa aggaatagcc ccacttgaaa aaaatggagc aatcaccatc
     5281 attgctgtca tacgcaccag caccagaaag aatgcctgaa actgatatac aaaatattcc
     5341 attatccggc acccaattta gaaatcatca tgaataatcc ttttgtatac gcaaccagtg
     5401 tatgaagaat ccacgctgca aacagtatca aagcagcaaa gat
//
LOCUS       DAOSZP010000072         5436 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_12625_length_5436_cov_3.818807, whole genome shotgun sequence.
ACCESSION   DAOSZP010000072 DAOSZP010000000
VERSION     DAOSZP010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5436
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12625_length_5436_cov_3.818807"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            406..834
                     /locus_tag="PK348_02530"
     CDS             406..834
                     /locus_tag="PK348_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004460550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HXK65121.1"
                     /translation="MAGKSDSINSTIGEGSVFEGKFYISGSLRIDGKFEGEIKTDEEL
                     VVGETGKVKTNIHAKNVVVAGTVIGNIIADEEVKLLETAKVLGDIETPILTVQRGVVA
                     QGMVTITGGQKKDVKKLIEESYAGAPQAQTGIFGKKGQER"
     gene            866..1876
                     /locus_tag="PK348_02535"
     CDS             866..1876
                     /locus_tag="PK348_02535"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HXK65122.1"
                     /translation="MKHWQLHSYSFGSLSITVLSAEIIIIFRGTKTTIIKSIPFKKIK
                     SIALVCGTIVIGVILFTGYVRSHHVFNSEAPWYDEIKKKAIASKDFEEPDKKKPLKIV
                     QHTVKSGETLSEIAQMYGVSMDTICGCNRLESYDLVHVGQVLKIPNKDGLLVSVQRGQ
                     TLPALAKKYNVAINKIVEENSFANPDFINIGQDIFIPDAKPLDIFKGFLWPVHTKNIT
                     SGYGWRKDPFYGESQFHQGIDVRANYEWVRASKYGRVSYAGWLGGYGKVVVVTHPGGD
                     KTLYGHLSKITVREGQYVKQGQIIAKSGNTGNSTGPHLHFEIIRKGQHINPYAELKKK
                     RK"
     gene            1913..3985
                     /gene="glyS"
                     /locus_tag="PK348_02540"
     CDS             1913..3985
                     /gene="glyS"
                     /locus_tag="PK348_02540"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HXK65123.1"
                     /translation="MANCNFVCEIGTEEIPAGYIPSAIEQVEKIVTEGLQSYRIAFGK
                     CTVYATPRRVAILVYDMADSQSEDYEELKGPSVKAAYDDRGNPTRALTGFLEGNKIKL
                     EDTYIQKTSKGDYIYAKRKVEAQPATTIIPVIIEKIINELNFPKRMRWNVKRVSFPRP
                     VNYICVLFNDAVVPYSIDGIDFDNKVRGHYIQYNRMIPLSRCDEYIPTLENNGVIVDH
                     NKRKAIIHEQLQNAAKQLGGVLVEDDELLTTVTFLTEMPYVVIGSFDEGFLSIPDIVL
                     ITEMKEHQKYFAVRDSKGNLLPKFLVISNNPPTEHIVKGNQRVIRARFSDARFFFEED
                     RKVPLAGRVDMLKSILFHKELGSIYDKVERMQTIAKVICNELSLNTQITQKIQRAVML
                     SKTDLTTALVFEFTSLQGQIGKIYALLDGEDEEVSQAIDFQYRPRFQGDVLPEHIVSI
                     VVSLAEKIDNIFGSFSVGNIPKGSADPYALRRQAAAIVDMLVERTIHLDLANVFREVS
                     SQYSNGDKHVEPILEFINGRAKTFLYEKQFAFDEVNACLAVGCYDYYELFLRAQSIHE
                     FRKDKRFGDLLIAFKRMNNIVSAFVKKNPGYVFSFNPDALIIDEEKRLYSFFNNKEKT
                     IKEYIAENKYRELFLLLIEGKEIIDTYFDKVMVMDANTSIRDNRLGMLQYILTLFTTL
                     IDFSQIQE"
     gene            3989..4654
                     /locus_tag="PK348_02545"
     CDS             3989..4654
                     /locus_tag="PK348_02545"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXK65124.1"
                     /translation="MKVCDGVYAYIWRGVFENNCNTFYLGDPFNMFIDPGLAKYVDMR
                     FADMKKDGIDTEKIAYIVATHSHPDHFEGCQQFMGKGPKVAMHKEEIAFLNEIGPQFF
                     AMFGMKFPQFTFDIELEEGPWKIGNEVLQVYKTPGHSPGSVCLYWESKKVLVCGDLIF
                     KESVGRTDFPGGSSALLKESIERMKELDIEYLLPGHMDIVVGKSNVKKNFELIEKYIF
                     PML"
     gene            complement(4655..>5436)
                     /locus_tag="PK348_02550"
     CDS             complement(4655..>5436)
                     /locus_tag="PK348_02550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65125.1"
                     /translation="EEEYHYSSKNIEDYARKRGYLKKGKTFNFKECFSDWFYTYFSRC
                     AARQSRTTRCAMQHSGSITPQSMFAFLRDHGSDHEYISPDKTDMGTVCMHASLLPTRP
                     SQSVAAFVAHLRKDTPVFWATGSSGTCTSVFMPFYLIGKEIDYFPANETSTYSPDVYW
                     WMHEKIHRQAIFKWDYFQRTIAPAIHTLEDEFIKKENELYKKKNTSSQKYFDFSKQCV
                     EKVIELHDRWDKELKAAPASPTKLSFKLFWNGQTKKAMIPL"
BASE COUNT         1725 a          876 c         1232 g         1603 t
ORIGIN      
        1 ttctcctcac tgcgttcgtc gaaatgacag agtaaagaac gtgcgtcgaa atgacagggt
       61 aaagaacgtt agtcgaaatg acagagtgta aagaacgtta gttcaaataa cgtggtgttt
      121 caattactta ttgacatcct tacatcaaat cagtcataca atccgtattc agggtgtcta
      181 ttgtgcaaaa ctttatttat tggtattggt acacgttgaa accttaaggc tattcaacag
      241 taactatcgc acaaaatatg taattgctaa aaatatgcaa taataaaaat taccggggga
      301 ttaattgatt tacaataaaa aatggttgaa ataaaattgc aaaaatctga gagtacgtgt
      361 aaattgtaac ggtgatgaaa tttataatcg tggggtgtta acagtatggc aggtaaaagt
      421 gatagtataa acagcactat tggtgaaggt tctgtttttg aaggaaagtt ttatatcagc
      481 gggtcactgc gaattgacgg taaatttgaa ggtgaaataa aaactgatga ggaacttgtt
      541 gttggcgaaa ccggaaaggt aaaaaccaat attcatgcaa agaatgttgt ggttgctgga
      601 actgtaatag gaaatattat tgccgatgaa gaagttaaac ttttagaaac agcaaaagtg
      661 ctgggtgata ttgaaacgcc aattcttact gtgcagcgtg gtgtggtggc acaaggcatg
      721 gttaccatta ctggcgggca aaagaaagat gtaaaaaaac ttattgaaga atcgtatgca
      781 ggggcaccac aggcacagac aggcatattt gggaaaaaag gccaggaaag atgattcgtg
      841 aaacagaaat accgataatt acactatgaa gcactggcaa ttacatagct attcgtttgg
      901 aagtctcagt attactgttt tgtctgctga gatcattatt atattcaggg gcacaaaaac
      961 tactattatt aagagtatac catttaaaaa aattaaaagc atagctttag tgtgtggaac
     1021 tattgtgata ggggttatac tgtttacagg gtatgtccgc tctcatcatg ttttcaattc
     1081 tgaggctcca tggtatgatg aaataaagaa aaaagcaatt gcaagcaaag actttgaaga
     1141 acctgataag aaaaaaccgc taaagattgt tcagcatact gtgaaatcgg gtgaaacatt
     1201 aagtgagatt gcgcagatgt atggcgtatc catggatact atctgcggtt gcaacaggct
     1261 ggaatcgtat gacctggttc atgtaggtca agttttaaag ataccaaaca aagatggtct
     1321 gctggtaagt gtacagcgtg gtcaaacatt acccgctctg gcaaaaaaat ataatgtggc
     1381 aataaacaag attgttgaag aaaacagctt tgcaaatcct gattttataa atattgggca
     1441 ggacattttt attcccgatg caaaaccact ggatattttt aaaggatttt tatggcctgt
     1501 gcacacaaag aatattacca gcggttatgg gtggcgcaag gatcctttct atggtgaaag
     1561 ccagtttcat caggggattg atgtgcgggc taattatgaa tgggtgcgtg caagcaaata
     1621 tgggcgggta agctatgcag gctggctggg ggggtatggc aaggtggtag tggttactca
     1681 tcctggtggc gataaaacct tatatgggca tctttcaaaa ataactgtcc gtgaggggca
     1741 gtatgttaag cagggacaga ttattgcaaa aagtggcaat accggcaatt caacaggacc
     1801 acatcttcat tttgagataa tccgtaaagg gcagcacatt aatccatatg cagagttaaa
     1861 aaagaaacgt aaataatata ataccaaata tattcactga caggacataa gtatggctaa
     1921 ctgtaatttt gtatgcgaaa ttggcactga agaaattcca gcagggtata tcccatcagc
     1981 tattgaacag gtagaaaaaa ttgtaacaga aggtttacag tcatatcgca ttgcatttgg
     2041 gaagtgtacg gtgtatgcaa caccacgtcg tgttgcaata ctggtatatg acatggccga
     2101 tagtcagtcg gaagattatg aggaactcaa aggtccttcg gtaaaagcag cctatgatga
     2161 caggggtaac ccaaccaggg cattaaccgg ttttcttgaa ggaaataaaa ttaaacttga
     2221 ggatacatac atacagaaga ccagcaaagg tgattatatc tatgcaaagc gtaaagttga
     2281 agcacaacct gctacaacaa ttattcctgt tattattgaa aagattatta atgagctaaa
     2341 ttttccaaag cggatgcgct ggaacgttaa acgtgtatca ttcccgcggc cagttaacta
     2401 catatgcgta ctttttaatg atgcagtggt tccctattca atagatggca ttgattttga
     2461 caataaggtg cgaggtcatt atatacagta taaccgtatg atacctttat cacgatgtga
     2521 cgagtatatt cccacgctgg aaaacaacgg ggtcatagtt gatcataata agcgaaaagc
     2581 tataattcat gaacaattgc aaaatgcagc aaaacaattg ggtggtgtgc ttgttgagga
     2641 tgatgaattg cttaccactg ttaccttttt aacagaaatg ccatatgtgg taataggcag
     2701 ctttgatgaa ggattcttat ctatacctga tatcgtactg ataactgaga tgaaggagca
     2761 ccagaaatat tttgctgtgc gcgatagtaa agggaattta cttcctaaat ttttagttat
     2821 atcaaataac ccaccaacag agcatatcgt aaagggaaat cagcgtgtaa ttagagcacg
     2881 gttttcagat gcgcgtttct tctttgaaga agaccgtaaa gtcccgcttg caggcagagt
     2941 ggatatgctt aaaagcatcc tgttccataa ggagttgggt tctatctacg ataaagttga
     3001 aaggatgcaa acaatagcaa aggttatttg caatgagtta tcgcttaata cgcaaattac
     3061 ccagaagatt cagcgtgctg tcatgctatc caaaactgac ctgacgacag cactggtgtt
     3121 tgagtttacg tccctgcagg ggcaaattgg caaaatatat gcgctgcttg atggcgaaga
     3181 tgaagaagtg tcgcaggcta ttgatttcca gtacaggccg cgttttcagg gggatgtgct
     3241 tccggaacat attgtgagta ttgttgtttc gctggctgaa aaaattgata acatatttgg
     3301 ttcgttttct gttggaaata tccccaaagg ttctgccgac ccatatgcac tgcgacggca
     3361 agctgctgca attgtggata tgcttgttga acgaacaatc catcttgatt tagcaaacgt
     3421 attcagggaa gtttcatcgc aatatagtaa tggggataag catgttgaac caatacttga
     3481 gtttattaat ggcagagcca agacattttt atatgaaaag cagtttgcct ttgatgaggt
     3541 taatgcatgt ttagcagttg gctgttatga ttactatgag ctgtttctca gggctcagag
     3601 cattcatgaa ttccgtaagg ataagcgttt tggtgatctt ttgattgcat tcaaacgcat
     3661 gaacaatatt gtcagcgcat ttgttaaaaa gaatcctggt tatgtctttt catttaatcc
     3721 cgatgcgctg ataatagatg aagagaaacg tttatatagt tttttcaaca ataaggaaaa
     3781 aaccataaaa gaatatattg cagaaaacaa gtatagagaa ttgttccttt tgcttattga
     3841 aggtaaagag attatagata catattttga taaagtcatg gtgatggatg ctaacacttc
     3901 gattcgcgac aacaggttgg gaatgttaca gtatattttg acactgttta ccacacttat
     3961 tgatttttca cagattcagg agtaatgtat gaaagtatgt gatggtgtgt atgcatatat
     4021 atggcgcggg gtttttgaaa ataactgcaa tacattttat ttaggtgatc ctttcaacat
     4081 gtttattgat ccaggccttg caaagtatgt ggatatgcgc tttgcggata tgaaaaagga
     4141 tggaattgat actgagaaaa tagcgtatat tgtggcaacc cattcgcatc ctgatcattt
     4201 tgaaggatgc cagcagttta tgggcaaagg tcctaaagtt gcaatgcata aagaggagat
     4261 agctttttta aatgaaattg gccctcagtt ttttgccatg tttggcatga aatttccaca
     4321 atttacattt gacattgagc tggaagaagg cccttggaag attggcaatg aggttttgca
     4381 ggtgtataaa acgccagggc attcaccagg ttcagtttgt ttatactggg aatcaaaaaa
     4441 agtgcttgta tgtggtgacc tgatattcaa ggaaagtgta ggccgaacag attttcctgg
     4501 cggcagcagt gcattgctga aagaaagtat tgaacgaatg aaagaacttg atattgaata
     4561 cctgttgccc gggcacatgg atatagtggt tggaaaaagc aacgttaaaa agaactttga
     4621 gctgatagaa aaatatatct tccccatgct gtaattacag gggtatcatg gctttttttg
     4681 tctgtccatt ccagaatagc ttaaaggaca attttgttgg ggatgccggg gctgctttca
     4741 gttccttatc ccagcgatca tgaagttcaa tgaccttttc tacacattgt tttgaaaaat
     4801 caaaatactt ctggctactg gtattcttct ttttgtacag ttcattttct tttttaataa
     4861 attcatcttc aagtgtatgg atagcagggg cgatagttct ctggaaataa tcccatttga
     4921 aaatggcctg acggtgaatc ttttcatgca tccaccagta cacgtcaggt gagtaagtgc
     4981 ttgtttcatt tgccgggaaa taatctattt cttttccaat aagataaaat ggcatgaaga
     5041 cgcttgtaca ggttcctgac gagccagtag cccaaaagac tggagtgtct ttgcgaagat
     5101 gggcaacaaa tgcagcaacg gattggcttg gacgggtagg aagtagtgat gcatgcatac
     5161 acactgttcc catatcagtt ttatctggtg aaatatattc atggtcgctg ccatgatcac
     5221 gtaaaaatgc aaacatactt tgaggtgtta tggatccaga atgttgcatt gcacaacgtg
     5281 ttgtccgtga ttgccgtgca gcacatcgtg aaaagtatgt ataaaaccaa tcagaaaagc
     5341 actctttaaa gttaaaggtt ttgccttttt ttagatatcc tcttttgcgg gcataatcct
     5401 caatattttt tgatgaatag tggtattcct cttcta
//
LOCUS       DAOSZP010000073         5435 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_12629_length_5435_cov_3.577881, whole genome shotgun sequence.
ACCESSION   DAOSZP010000073 DAOSZP010000000
VERSION     DAOSZP010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5435
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12629_length_5435_cov_3.577881"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            105..2345
                     /locus_tag="PK348_02555"
     CDS             105..2345
                     /locus_tag="PK348_02555"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HXK65126.1"
                     /translation="MMISIQKFETWAIKPFFAIFNFISERITYTFAIAFTTSLGGFII
                     AAYIALFLYATVRFDVSNTGQDFVILFSGMTLFIYAPLYYIHFGLLYNAGVPCFTKRL
                     SLINTYIVHNTLHQTIDDTEAVRLLEALEHLPRDNMTAALIYPSIVMAGVCVYEAIKG
                     AFTNALFLLLGISSAIFIYVFLTYIVAELLSGRMRRKVKWLLVKRGISFNERYILSIR
                     GKFIFINIMVLVAMLEIGLMIYYNNYDIFSPIPILFIVITGFIVFVILFFYLLSIEES
                     LSEIESAAIDLARGGSGSLFLPYLDKELINTSKGFLAAAYEVNDIRRNLEQKVEERTK
                     ELNQALTALKEKNDLIAKELKIAASIQQGILPQTPVVFNAIKVLAYNRASSDIGGDLY
                     DIIRMPNDCLGVLIADVSGHGIPAALISTMVKISFHESCRKYLLPRRIFADVNNQLVN
                     IIRTQEYATVFFMVIFPTYDVMYANGSHRKALVCKRNSGRLEYWDTGGIFLGALPNEE
                     IGGLFEDNFGILEPGDRVFLYTDGLPESRNWNNEEFGDERVEKLLLETLDMPIDDAKE
                     YIIEQWEKFLGGTPPRDDMTFVLLELDPIMHSALQHREKGQELYAQKKYTLAIDELKR
                     GVIMNPEDMTSLLLLARSYIYTRRYSNAVVHLTEYVAKKPDDSMAQYLLAIAQYKLHN
                     YEEAYKAATHSLALRPSYIPALYVMAAICASLHKTSDAIEYCNKILEADPGNVKAQKL
                     LQKLKH"
     gene            complement(2342..4159)
                     /gene="pepF"
                     /locus_tag="PK348_02560"
     CDS             complement(2342..4159)
                     /gene="pepF"
                     /locus_tag="PK348_02560"
                     /inference="COORDINATES: protein motif:HMM:TIGR00181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HXK65127.1"
                     /translation="MQKNAKQIPARNEIPAEHTWDLMPLFVSDSNWEEVYSNTEQLIP
                     RYKNYEGKLADSFDVFLQAIEFDLSIARDIDRLYTYAHLRSDEDTTNQNAQAMLQKAH
                     SLAQRAAEASSFLVPEIQSLPEAVISNYLTNTNISQYVFYIEKILRYREHTLSKEIEE
                     ILAMAHEVVIAPMEFFRKLDNADMRFGTITDEQGNTVELSHGNFITFLMSYNQDVRKN
                     AFHTYYKAYDNHKYSIAQSLASSVKKDVFFSRVRKFKSCLHQALFPDNIPDTVYHTLI
                     ETVSSNLQPLFKYLSYRKEALGLTELHFYDTYVPLVADVPFSMEYEEACDVCAQALHI
                     LGEEYISTLHTGLLGGWVDRYENRGKVSGAYSSGCYDSPPYILLNYDANTINSLYTLI
                     HEAGHSMHSYYSRKNQPYVYHEYTIFVAEVASTLNEILLSHYLLQQYSDNPRMQAYIL
                     NREIDEIRGTLFRQTMFAEFELLVHTMAENNKPLTFQTMTDTYTNLLHKYFGTTIVID
                     TDLYLEYLRIPHFYSAFYVYKYATGIAAALSLAKQIINNPDTSQRYLQFLSLGGSTFP
                     LEELRIAGVDMESPEPVIDALQYFDSLVDRFIDVHKKIH"
     gene            complement(4190..>5435)
                     /gene="nhaC"
                     /locus_tag="PK348_02565"
     CDS             complement(4190..>5435)
                     /gene="nhaC"
                     /locus_tag="PK348_02565"
                     /inference="COORDINATES: protein motif:HMM:TIGR00931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HXK65128.1"
                     /translation="IKSYFLGHNWSEIEGSIVNGIKGTLPAIMILLTVGILSGSFIAS
                     GTIPLLIDYGLKLIHPSWILPAAYIVCCISSLVIGSSWTTAATIGVALVGMAGAMHIS
                     PAICAGAVVSGAYFGDKLSPLSDTTNLAPGVSETDLFSHIRAMMATTIPAWSLALTGF
                     IVLNIYIVPDTTTRIEHIESLLQTLESSFNLHPVTILPLAVVFIMALVRFPAVVILLS
                     GSTAAILVGWLAQSQSLLSVLQYAYSGYTIHTNNEIINNLLNRGGIVSFLETTLIILC
                     ATGLGGIWESGGYITSLHDNLLHKIKTSRMLIISTVITAVISNIIMAEQYLSIVMTGR
                     IYKDAYKRKKLQPFMLSRSLEDGGTVTSPLVPWNSCGAFMSSALGVPTLHYLPFAFFN
                     LLMPVVAIVLTAFGLFIYRKND"
BASE COUNT         1618 a         1022 c         1136 g         1659 t
ORIGIN      
        1 ctgtgaaata taaaaatttt tttaattgat ttttttagta tgatatacta caatttacaa
       61 gaatatacta aatatctaaa atacttgtac tatcaggata gttgatgatg atatccatac
      121 aaaaatttga aacatgggca attaagcctt tttttgccat atttaatttt ataagcgaac
      181 ggattaccta tacctttgct atagcattta ctacaagttt gggtggcttt attattgcag
      241 cgtacatagc actttttttg tatgctactg tcaggtttga tgttagcaat acaggccagg
      301 atttcgtaat ccttttttca ggtatgacgc tttttatcta tgctccctta tattatatac
      361 attttggatt attgtataac gcaggagttc cctgttttac aaaacggctt tctctcatta
      421 atacgtatat agtacataat acgcttcatc agacaattga tgatactgaa gcagtacgat
      481 tgctggaagc tcttgaacat ttgccgcgtg ataatatgac ggcagcatta atttatcctt
      541 caattgtgat ggcaggagta tgtgtgtatg aagccataaa aggggctttt actaatgcat
      601 tatttctttt gcttggtatt tcttcggcaa tattcatcta tgtattttta acctatattg
      661 ttgccgagct tttatcggga aggatgcgcc gtaaggtaaa gtggcttttg gtaaagcgtg
      721 gcatttcttt taatgaacgc tatatcttaa gtatacgggg aaaatttata ttcattaata
      781 tcatggtcct ggtggcaatg cttgagatag gacttatgat atactataat aattatgaca
      841 tattttcacc aatacccatt ttgtttattg ttatcacagg atttattgta tttgttattt
      901 tgtttttcta cctgctgtca attgaagaat cattgagtga aattgagtct gctgcaatag
      961 atcttgcgcg gggtggcagc ggttcattgt ttcttcccta tctagataaa gaacttatca
     1021 acaccagcaa aggcttttta gctgctgcgt atgaggttaa tgacatacgt agaaatctgg
     1081 aacaaaaggt tgaagagcgg acaaaagaat tgaatcaggc cctgacggca ttgaaggaaa
     1141 aaaatgattt aattgcaaaa gagttaaaaa tagctgccag cattcagcag gggatattgc
     1201 cgcaaacacc ggttgtgttt aatgcaataa aggttcttgc atacaaccgt gcttccagtg
     1261 atattggcgg tgatttgtat gatatcatac gcatgcccaa cgactgctta ggggttttaa
     1321 ttgccgatgt atccggtcat ggtattccgg ctgcacttat tagcaccatg gttaaaataa
     1381 gcttccatga atcatgccgt aaatacttgc ttccacgacg catatttgct gatgtcaata
     1441 atcagcttgt caatattatc cgcacgcagg aatacgcaac agtatttttc atggtaatat
     1501 ttcccaccta tgacgtcatg tatgcaaatg gatcacaccg taaagcgctt gtgtgtaaac
     1561 gcaatagcgg tcgtctggaa tactgggata ccggtggaat atttttaggt gctcttccca
     1621 atgaagagat tggcgggctt tttgaggata attttgggat actggaacct ggtgacagag
     1681 tatttttgta tactgatggg cttccggaat cacgtaactg gaacaatgaa gagtttggcg
     1741 atgaacgggt tgaaaaactt cttttggaaa ctcttgatat gcctattgat gatgcaaagg
     1801 agtacatcat tgagcagtgg gaaaaatttt tgggtggcac accaccacgg gatgacatga
     1861 catttgtgct gcttgaactt gatcccatca tgcatagtgc actgcagcat cgtgaaaaag
     1921 ggcaggagct ttacgcccaa aagaaataca ctcttgcaat agatgaactg aagcgtgggg
     1981 tcattatgaa tccagaagat atgacctcat tattgctatt agcccgttca tatatttaca
     2041 cacgccgcta cagtaatgct gtggttcact taactgagta tgtggcaaaa aaacctgatg
     2101 acagcatggc tcaatatttg ttggctatag ctcagtataa gttacacaat tatgaagaag
     2161 catataaagc tgcaacccac tcgcttgcct tacggccatc atacattcca gcgctttatg
     2221 ttatggctgc tatttgtgct tccttacata aaaccagtga cgccatagag tactgtaaca
     2281 aaatacttga agctgatcct ggcaatgtta aagcgcaaaa gctgttacag aagctgaagc
     2341 attaatgaat ttttttgtga acatcaataa acctgtctac aagactgtca aaatactgta
     2401 acgcatcaat aacaggctct ggtgattcca tatccacacc cgcaatgcga agttcttcta
     2461 aagggaatgt actgccacct aatgacagga attgaagata acgctgtgat gtatcgggat
     2521 tgtttataat ctgtttagct agtgaaagtg cggcagcaat tcctgttgca tatttgtaca
     2581 cgtaaaacgc tgaataaaaa tgtggaatac gcaggtattc caggtataga tcggtatcaa
     2641 taacaatggt ggtaccaaaa tacttgtgta acaggtttgt gtatgtatca gtcattgttt
     2701 gaaatgtcaa tggtttgttg ttttccgcca tggtatgcac cagtagctca aactcggcaa
     2761 acatggtttg cctgaatagt gtgccacgga tttcgtctat ctcacggtta agaatgtaag
     2821 cctgcattcg cggattatca ctgtactgct gcaaaagata atggcttaaa agaatttcat
     2881 tgagtgttga tgcaacctct gcaacaaata tagtgtactc atgatatacg taaggctgat
     2941 ttttccgtga atagtatgaa tgcatggagt gtcctgcttc atgtatcagt gtgtaaaggc
     3001 tatttatggt atttgcatca tagtttaaaa gaatatacgg tggtgagtca taacagcctg
     3061 atgagtaagc accgctaacc tttcctctgt tttcatagcg gtcaacccag ccaccaagga
     3121 gccccgtatg caacgtagaa atgtattctt caccaagtat gtgcagggcc tgcgcgcata
     3181 cgtcacaggc ttcttcatat tccatgctaa atggcacatc ggcaacaaga ggaacgtagg
     3241 tatcatagaa gtgcaattca gtaagcccca atgcctcttt acgatatgaa agatatttaa
     3301 acagtggttg caggttagaa gatactgttt caatcaatgt atggtacaca gtgtcaggta
     3361 tattatcagg aaacagtgcc tgatgcaggc agcttttaaa ctttcgcaca cgtgaaaaaa
     3421 atacatcttt ctttacagag ctggccagtg actgtgcaat gctgtatttg tgattgtcgt
     3481 aggctttata ataggtgtga aaagcattct tcctaacatc ctgattatag ctcattaaaa
     3541 atgttataaa gttcccatgg cttagctcaa cggtattacc ctgttcatcg gtaattgtac
     3601 caaagcgcat gtcggcattg tcaagttttc ggaaaaattc cattggtgca atgacaacct
     3661 catgagccat ggctaaaatt tcttcaattt ctttggataa tgtgtgttcc ctgtagcgaa
     3721 gaatcttttc tatataaaag acatactgag aaatgttagt gtttgtcaga tagttactga
     3781 taacagcctc aggcagtgat tgaatttcag gtactaaaaa gcttgaggct tcagctgcac
     3841 gctgggctaa cgagtgagct ttctgcagca ttgcctgtgc attctggttg gtggtgtcct
     3901 catcgcttct tagatgagcg tatgtataga gcctgtcaat atcacgtgct atggaaaggt
     3961 caaattcaat tgcctgtaaa aatacatcaa aggaatctgc cagcttccct tcatagtttt
     4021 tatatctggg aattaattgt tctgtgttac tgtagacttc ttcccagtta ctatcggaca
     4081 caaacaatgg cattaaatcc catgtatgtt cagctggtat ttcattgcgt gcaggtatct
     4141 gctttgcatt tttttgcata gtacaatctc actgtaaaaa agtattgttc taatcattct
     4201 ttctgtaaat aaagaggcca aaagctgtca acacaattgc aacaaccggc atcaaaagat
     4261 taaaaaacgc aaagggaaga tagtgcagtg taggcacacc cagcgcagaa ctcataaatg
     4321 ctccgcatga attccacggt accagtgggg acgtgaccgt tccaccatct tccagcgaac
     4381 gggaaagcat aaaaggctga agttttttcc gcttgtatgc gtctttataa attcttcctg
     4441 tcataacaat ggataaatat tgttcagcca taatgatatt agaaataaca gcggtaataa
     4501 ccgttgaaat gatgagcatt ctgctggttt taattttatg aagcagattg tcgtgtagcg
     4561 atgttatgta acccccgctt tcccatatgc cacctaaacc tgtagcgcat agaataataa
     4621 gcgttgtctc taaaaagctt actataccgc cacggttcaa aaggttgtta atgatttcat
     4681 tattggtatg tatggtatac cctgaatatg catactgaag taccgaaagt aagctttgtg
     4741 attgtgccag ccatcccacc agtattgctg ctgtacttcc tgatagtaag ataacaacag
     4801 ccggaaaacg cactagtgcc ataatgaaca ctactgccag cgggagtatg gtgaccgggt
     4861 gcaggttaaa tgatgattcc agggtttgta ataatgattc aatgtgttct atacgggtag
     4921 tagtatccgg aacaatgtat atgtttaaca caataaaacc agttaatgcc agtgaccatg
     4981 ccgggatagt ggttgccatc attgcccgaa tatgtgaaaa caggtcagtt tcagaaactc
     5041 ctggtgctaa atttgttgta tcggataacg gcgaaagctt atcgccaaaa taggcgcctg
     5101 aaacaacagc accagcacag atggcagggg atatgtgcat ggcaccagcc atcccaacaa
     5161 gcgccacacc aatagtagct gcagtagtcc aggaacttcc aatgacaagg ctgcttatac
     5221 agcacacaat gtatgctgcg ggaagaatcc atgatggatg aataagcttc aaaccataat
     5281 ctataagcag aggaatagtt cctgatgcta taaaactgcc tgataatatt ccaacagtca
     5341 aaagaatcat gatggcaggt aatgtgcctt ttataccatt gacaatactg ccctcaattt
     5401 ctgaccagtt gtgacctaaa aaatagcttt ttatg
//
LOCUS       DAOSZP010000074         5399 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_12728_length_5399_cov_3.195921, whole genome shotgun sequence.
ACCESSION   DAOSZP010000074 DAOSZP010000000
VERSION     DAOSZP010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5399
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12728_length_5399_cov_3.195921"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..494)
                     /locus_tag="PK348_02570"
     CDS             complement(<1..494)
                     /locus_tag="PK348_02570"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXK65129.1"
                     /translation="MSTKIAIITGAAGGIGRAITAEFIKKNITCVLVDINKKALADLQ
                     KQYGDKVYGLYCDITEIKSVKSTVNKAVKKYGAIDILVNNAGIIEPNLFENASYKEIM
                     RQIQVNLMGPINCTYEVLPYLKKSKSPSIVTIASLAGIVPETYSSLYTATKFALRGLF
                     LTLH"
     gene            complement(865..1767)
                     /locus_tag="PK348_02575"
     CDS             complement(865..1767)
                     /locus_tag="PK348_02575"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXK65130.1"
                     /translation="MKRKLWISISFVVTLLVFVYAQNPSRLKESGFNSAFTIPLETPY
                     EQYVELMTHVIANARVETNNKDAIVAMNSPFIFKPQVKPVNGIYPKGILLIHGLSDSP
                     YFLRAVGKYFTEKGFLVYGLLLPGHGTVPGDLLKVKNDEWIKATHYGMLQLKKNAKDV
                     YMGGFSTGALLSVHYVLSNNNHDVKGLFLFAPAFAIYSKLAWVAPIVGTFKDWESVED
                     DTDYTKYQSFTFNGVTQTYRLIKKVDELFDEGKRIQVPVFVAQSIDDRTVSTERTLFV
                     MKNYVTGNKMFVLYTTQPEKDYDG"
     gene            complement(2033..2959)
                     /locus_tag="PK348_02580"
     CDS             complement(2033..2959)
                     /locus_tag="PK348_02580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65131.1"
                     /translation="MLLTRYSGKIIFSLLVFLLLSSPTLFAQTQIDLNPSAKTIDGIT
                     NIAASIVADSFVPMTHYNEDNYMVSIVPAYFTVQKVYDDPEINGNDVKGYGAGTGFGY
                     ATSDRMLIYGIAAYMHFDGRLSSAFLKGLDANIYQMKVDYSIISLYSGIGYDVVESSE
                     FSLPLFAGIFIQRYNGTGSLPEILNTKLSVDSSATLFGVSFGIAPSFKYNDLFKITPY
                     YLVSYSLNKPDATAKITTTVPFPITANYDVPANNIKASMVGMALTWLSSKSISLSLSL
                     GGWLRNETSWYNKTFLNGLQMKSAVLVVSFSN"
     gene            3519..5057
                     /locus_tag="PK348_02585"
     CDS             3519..5057
                     /locus_tag="PK348_02585"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HXK65132.1"
                     /translation="MSILQKFLRIYDNKDYLTRQQAEALFILYFIVALGLAAMGVSMF
                     IIYKSYHPTHVGVLLCEIVLSISFFLNYKGKNIAAGYLMLFPLNIVLWYIIWIITGVD
                     DTITVTDSIFYVFPLIATATLINNRTSIIVFTIFNVLSHGAYTVYFHKTGVLNAKQFL
                     DLLQDGIIAISVFGGICVSFITMKMKSHALILNSAKEAQDNKSKIEEMLQQTYSVSKD
                     LAVSTSHLVGMTDNFSQATQSQASTLEEITSSVEEVTASGEGVLAMVQHQSGMGDYVK
                     NNMDSLYQIVTEVGEKIKEAIEIRNVLNDLVGQSRNEIQITLNDVHNALSQFHDMKNT
                     VEIIEDISDKINLLSLNAAIEAARAGEFGRGFAVVAEEIGKLADNTQANLKIINEMFN
                     KSNIGITQASKRLDEFIHLLNEMIGNIEKLSGSIDEVVKLARDDLNLNRQTRNSLEEL
                     LQEAGNILRATNEQKQALDEIAKSINVFNETIQDIASSAQNLSGFSVDLASMVQKLKS
                     MSGI"
BASE COUNT         1755 a          921 c          936 g         1787 t
ORIGIN      
        1 atatgcaatg tcaaaaataa accacgcaat gcaaactttg ttgctgtata tagtgaactg
       61 taggtttcgg gcactattcc agcaagtgag gcgatagtta ctattgatgg gcttttgctt
      121 ttcttcaaat atggcaatac ctcatacgta caattgattg gccccattaa attaacctga
      181 atctgacgca ttatttcttt ataggaagca ttttcaaaaa ggtttggttc aattattcct
      241 gcattattga caagtatatc gatagctcca tactttttta ctgctttatt aaccgttgac
      301 tttactgatt taatttcagt tatatcacag tacaggccat aaactttatc accatactgt
      361 ttttgcaaat cagccagcgc ttttttatta atatctacaa gcacgcacgt tatattcttc
      421 tttataaatt cagcagttat agccctacct ataccgcctg ccgcaccagt gataattgca
      481 atttttgtac tcatagtatt gtacctctat tttaatatat gtagcgatgc aacgtattgc
      541 accacaatat gtgtgttatt tttcaattac tgataaaaat tcacccattt gtttgcacat
      601 actattaaaa tgcggattat acgtcaaccg tgtaataact ttatactttt caatattttc
      661 ttcggtattt tcgcctttcc atatttcagg atctgataaa cactgctgtc gttcaggact
      721 atttggtttg taatataaac aattacggta atcgccattt ttgccgtagt gcggatcatc
      781 gggtggtata gtgatacata tatgcggtat atttaaaatt ttctcttgag gaatataact
      841 gtttacagtg ataacaaaat tatcttatcc atcataatct ttttcaggct gtgtggtata
      901 tagaacaaac atcttattac ccgttacata atttttcata acaaagagcg ttcgttcagt
      961 acttactgta cgatcatcta tgctttgagc aacaaacaca ggaacctgaa ttcttttgcc
     1021 ttcatcaaac aattcatcaa ctttctttat aagcctgtat gtctgggtta caccgttaaa
     1081 tgtaaatgat tgatacttgg tataatcggt atcatcttct acgctttccc aatccttaaa
     1141 tgtaccaaca ataggagcta cccatgctaa tttagaataa atggcaaaag ctggtgcaaa
     1201 caaaaagagc ccttttacat catgattgtt gttagatagc acataatgaa cagacaacaa
     1261 tgcgccagtt gagaatccac ccatatacac atcttttgca ttctttttaa gctgtaacat
     1321 gccatagtgg gtagctttta tccattcatc attttttact tttaataggt cgcctggtac
     1381 ggttccatgt cctggtaaaa gtaagccata aacaaggaac cctttttcag taaaatattt
     1441 tcccacagca cgtaaaaaat acggtgaatc cgacaggcca tggataagca aaatcccttt
     1501 tggataaatg ccattaacag gttttacctg tggtttaaag ataaatgggc tattcatagc
     1561 aacaatagca tctttattat ttgtttcaac acgagcattt gcaataacat gtgtcatcaa
     1621 ttcaacatac tgttcatacg gtgtttccag tggaatagta aaagcactgt taaaacctga
     1681 ttcttttaac cgtgaaggat tctgtgcata gacaaaaact aatagagtaa ctacaaatga
     1741 aatacttatc cataactttc gtttcatcat ttccccctaa tcgtagaatt tcacaaatgt
     1801 tattccgaat ctgattcgga atctattgct gtattaatat tctgaacctt ttcctgaaat
     1861 aaaattggga taaggttcag aatgactttg ctcataatat tttcatagta tggtttctta
     1921 gcccggtgaa gtatgattgt ttaccgcagt cataaatgcc cttatattgt ataatgattt
     1981 atatccaata ttgcttttgc aactactttt tatattgatt ttgttagagg ttttaattac
     2041 taaaactcac taccaaaact gctgatttca tctgtaaacc gtttaaaaat gttttgttgt
     2101 accatgatgt ttcattgcgt aaccagccac caagactaag cgatagtgat atcgatttac
     2161 tgctgagcca cgtaagtgcc ataccaacca tggaagcttt tatattgttt gctggtacat
     2221 catagtttgc agttattggg aaaggtacag tagtagtgat ttttgctgta gcatcaggtt
     2281 tattcaggct gtagcttaca agataatatg gtgttatttt aaacaagtca ttatatttga
     2341 aggaaggagc tataccaaac gaaacgccaa ataaggtcgc agagctgtca actgatagtt
     2401 tagtgtttaa aatttcaggc aatgaccccg taccgttata tctctgtata aatatacccg
     2461 caaacagggg gagtgaaaat tcactggatt caaccacatc ataccctatg cctgaatata
     2521 acgagattat agaataatca actttcatct gataaatatt agcatccaat cccttcaaga
     2581 aagcagatga taatcttcca tcaaaatgca tatatgcagc aatgccatat ataagcattc
     2641 tatcgctggt agcataccca aaccctgttc cagcgccata cccttttacg tcatttccgt
     2701 tgatttcagg gtcatcatat actttttgaa cagtgaagta tgcaggaact atcgaaacca
     2761 tatagttatc ttcgttgtaa tgcgtcatgg gcacaaaact atcagcaact atggatgcag
     2821 caatattggt tattccatcg atagtttttg cggatgggtt aagatcaatt tgtgtttgtg
     2881 caaacaaagt gggtgagctc aggagaagaa atacaagtag cgaaaagata attttaccag
     2941 aatatcgagt tagtagcatc tagactcccc ctgattaagt tttttatttc tataaataga
     3001 tattagatat aacgctatca atgatgtttg cttatgatga cgaatggaac atattcatga
     3061 tatctatact gtaaagctga agtataagat gttgcagatc aaatagagta tcaatgaagt
     3121 ttcgtaacct taatagcgtt aagtatgaaa tttactaaaa ggatctgtca atgaaaatta
     3181 taaaaaaata tcattcttta ttactgaatg tgaaaatatg aataaaatat acatacagta
     3241 tactccagat gccttcggtg gcggaagtac ttgctttatg taatataaaa tttttttaag
     3301 gattattacc taaacaaata cactataact ccataatgga accgccatag ttcaatagaa
     3361 tgttgtctga tatgaagcat tgttcattgg ttttaaaata tcatgtaacc atgaatattg
     3421 gttgactatt atattatttt actatctgtt tggttattaa aatactgtta ttttcttaat
     3481 catccatgtt gtatattatt ttttaaggat gccttggtat gtctatactc caaaaatttt
     3541 tacgaatcta tgataacaaa gattatttaa cccgacaaca ggccgaggca ttatttattt
     3601 tatattttat tgttgctctt gggcttgctg caatgggagt atctatgttt attatctata
     3661 aatcttacca tcctacacat gtaggcgtgc tgttgtgcga aatagtctta tcgatttcat
     3721 tttttcttaa ttacaaagga aagaatattg cagcgggata tttgatgctt tttccgctta
     3781 atattgtgtt atggtatata atatggataa taacaggagt tgatgataca atcacagtaa
     3841 ctgattcaat tttttatgta tttcccctga tagctacagc tacgctgata aacaatcgca
     3901 cttcaattat agtatttaca attttcaatg ttctatcgca tggggcatat acagtctatt
     3961 tccataaaac aggtgtcttg aatgcaaaac aatttctgga tttattacag gatgggatta
     4021 tagccattag tgtgtttgga ggcatatgtg tttcttttat aacaatgaaa atgaaatcac
     4081 acgctctaat actaaattct gcaaaggaag cacaggataa taaatcaaaa attgaggaaa
     4141 tgttgcaaca aacatattca gtttcaaaag acctggcagt ttcaacctct catctggttg
     4201 gcatgactga caacttttca caggcaactc aatcgcaagc ttcaacactg gaagaaatta
     4261 cctcttcggt tgaggaagtt actgccagcg gtgaaggggt gctggcaatg gtgcaacatc
     4321 agtcaggtat gggggattat gttaaaaaca acatggattc tttatatcag atagtaaccg
     4381 aagtaggaga aaaaattaag gaagcaatag aaataagaaa tgtcctgaat gatttggttg
     4441 gccagtccag aaatgaaatc cagatcacgc tgaatgatgt gcataatgcg ctttcacagt
     4501 tccatgatat gaagaataca gttgagataa ttgaagatat ctcagataag atcaatttat
     4561 tatcattgaa tgcagctatt gaagctgccc gtgcaggtga atttggcagg ggctttgctg
     4621 ttgttgcaga agaaattggc aagttggctg ataacactca ggctaatttg aaaataataa
     4681 acgaaatgtt taacaaaagt aacatcggta ttacacaggc atcaaaaagg cttgatgaat
     4741 tcatccatct actcaatgaa atgataggaa atattgaaaa actatcgggc agtattgatg
     4801 aagttgtaaa attagcccgg gatgacctta atctcaacag gcaaacacgc aattcactgg
     4861 aggaactatt gcaggaggcg ggtaatattt taagagccac aaacgaacaa aaacaagctt
     4921 tagatgaaat agcaaaaagc attaatgtat ttaatgaaac cattcaggat attgcatcaa
     4981 gtgcacaaaa tctctctgga ttttctgttg atctggctag tatggttcaa aaattaaaaa
     5041 gtatgtcagg tatataaaca atcatttttc cggttaggtc tgtttgaaac tattgttgat
     5101 tggatcatat atatttttta acaacttcac ccatattaat gcaatgagcc gtcttttgta
     5161 ggcaaataac tttgggcagg cgcggctaac ttatataaaa acagcattgt acactatctc
     5221 atatttaaaa taatgacccg gtactataat tggcagataa aaaaatactt gacaaaattt
     5281 attttataac ggatgttcga tataaataaa ttaaattttg gaggttcgta agtttatgca
     5341 acagcaagaa tatgatgcaa ttattgtagg tactggtcca ggtggtgcaa ccgttgccc
//
LOCUS       DAOSZP010000075         5346 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_12879_length_5346_cov_2.962578, whole genome shotgun sequence.
ACCESSION   DAOSZP010000075 DAOSZP010000000
VERSION     DAOSZP010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5346
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12879_length_5346_cov_2.962578"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1118
                     /locus_tag="PK348_02590"
     CDS             <1..1118
                     /locus_tag="PK348_02590"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HXK65133.1"
                     /translation="KKEVGMAKVYFIDMHMSSRENISSKIKKLFYKLEVNSIINEKDF
                     VAIKTHFGEYGNIAYLPPAIIRAFVDCVKDLKANPFITDANTLYRGSRSNAIDHLKNA
                     EMNGFSIFSCGAPVIIADGLRGNDFKEIPASGKWYSSVKVASAIHDADAMIVVSHVKG
                     HELYGFGGAIKNIAMGCAPPSMKQSLHSDLKPKVKQALCTSCGACIKRCPAFAISFDE
                     NKKAFIDSNKCFGCGECTVICIYNAIPVVWKTDATALFERTAEYVDAFMQQKKNKVAF
                     FNFIMNVSPECDCCYWNDTPIVPNIGILASTDPIAIDRASVDLINKAMPLPGSRIALK
                     DASKDNITALYPVQWEYLFKYAASLGAGDEKYELIEI"
     gene            1167..1607
                     /locus_tag="PK348_02595"
     CDS             1167..1607
                     /locus_tag="PK348_02595"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXK65134.1"
                     /translation="MKSPHDFPAINPKKPDGIKPNGIPYKVMVVEDKEFHRKQIIQIL
                     ESEGYEVIAAATNGAEALKLYDSLAADLDLITTDLDMPKLDGYALLYELSQKKPKAKI
                     VFISDETTKGVLADLLKMGAADFILKPIQRGTILERVKAVLQKK"
     gene            1633..2709
                     /locus_tag="PK348_02600"
     CDS             1633..2709
                     /locus_tag="PK348_02600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65135.1"
                     /translation="MGENEKAYQHLSEQTKKINIALHYTHGDMEKARQMVAGNYNDLY
                     AIKVRFASSTMFGAFLLFFNHIYKIISGLTLILSPSYTLQNINPEWDWIVFEKEIAEL
                     MQKGDYDQILAKTFRDKIMKSLDIILLNELARNIELKNEIAIDNLFRKLIQETLALKR
                     VDSFVVYQPMSSLAMELHSVTIPKLKSEIVDGEQVTKPETQSTATGEAEYVPQVGVDG
                     VKLIIKSALILSPIKGKHISKLSPGDRVMISIIDTNPQAVKVAQALNAYENETFKPIP
                     ARITYVKYIPQKGYKVYGLIAKGIVVDIDEEEENIKVALDPAYAATIQSPEEEVKPNY
                     VLIAILLLAFGILMGILYLLLWSK"
     gene            complement(2711..3469)
                     /gene="hisF"
                     /locus_tag="PK348_02605"
     CDS             complement(2711..3469)
                     /gene="hisF"
                     /locus_tag="PK348_02605"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010880065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HXK65136.1"
                     /translation="MLAKRIIPCLDVKDGRVVKGVNFVNLQDAGDPVHNGMFYDEEGA
                     DELVFLDITASSDRRAIILKMVKEVAETVNIPFTVGGGIRTVDDVRMILENGADKVSI
                     NTQAVQQPELITECARRFGSQCIVVAIDAKRNNGGWNVYLHGGRTKTDIDAVQWAKQV
                     EDLGAGEILLTSMDRDGTKLGYDLELTKKVAESVTIPVIASGGVGTLKHLYEGFAIGK
                     ADAVLAASIFHYREYSIKQAKEYLAMRGIPVRPV"
     gene            3586..4653
                     /locus_tag="PK348_02610"
     CDS             3586..4653
                     /locus_tag="PK348_02610"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HXK65137.1"
                     /translation="MSKKNEYISFAEFSRTYVPAINEYIYTFYSNKELSAHNPDIKLA
                     YNLLKEYCTRDGKRIRPLLLLLSYLGYGGPISEIQSVIPVAASLECMHAFLLIQDDII
                     DGAIMRRGKPAFHKVCDSSYNKYNEHIGSWVAIVLADVLMSNALEMISGLTTTSMSAF
                     LHEFAIIYERTALGQMLDIIWSNPKYIDDENIPHAIASMKTAHYTVSGPLRLGYILAG
                     GSNNDELQKLEQAGYKVGFAFQMRDDIIGVFGVEEDSGKSASSDIEEGKYTVLVQKTL
                     QLLHDSTVKEQFMAIFKKKQKSKEDIQAIKAYIAKSGAKANVQQLISRYVNNAKEIIP
                     QLQCDSFMKRCLLDFIESLFN"
     gene            4713..>5346
                     /locus_tag="PK348_02615"
     CDS             4713..>5346
                     /locus_tag="PK348_02615"
                     /inference="COORDINATES: protein motif:HMM:NF024571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXK65138.1"
                     /translation="MIYKNRQLEYILKEASTTFPALLLTGPRQSGKTTLLKNLFSDKA
                     KFISLDESDIRLWAHEDPRDFIQNNMPPVIIDEIQYAPDLLTYIKRRIDDDRSPGQWF
                     LTGSSQFTLMKHVSESLAGRTAIFHLLPFSHSEIHGRVYPNWDDWFTSIKDNVPCDED
                     LGNVLLRGTYPELVCNATINRKIWYDSYIQTYIERDLSLVYNIGDINTFTK"
BASE COUNT         1724 a          937 c         1055 g         1630 t
ORIGIN      
        1 acaaaaaaga ggtgggtatg gcaaaagtat attttattga tatgcatatg agtagccgcg
       61 aaaatattag tagcaaaatt aaaaaattgt tctataaact ggaagttaac tcaattatta
      121 atgaaaaaga ttttgtagca ataaaaaccc attttggtga atatggcaat attgcatatt
      181 taccacctgc catcatacga gcttttgtag attgtgttaa agatttaaag gcaaatcctt
      241 ttattacgga tgccaataca ttgtatcgcg gttcacggag caatgcaata gatcatctga
      301 aaaatgctga gatgaatggt ttctctatat tttcgtgtgg ggcacctgtt atcatcgccg
      361 atggtttgcg tgggaatgat tttaaagaaa tccctgcttc cggtaaatgg tattcaagtg
      421 tcaaagtggc ttctgctatt catgatgcgg atgctatgat agtggtaagc catgtaaaag
      481 gacatgaatt gtatggtttt ggtggagcaa taaaaaacat agccatgggc tgtgcacctc
      541 cttccatgaa acagtcattg cattcggatt tgaaacctaa ggttaaacag gcattatgta
      601 catcatgtgg ggcatgtata aaaagatgtc ctgcatttgc tatttcattt gatgaaaata
      661 agaaagcatt tatagatagt aataaatgct ttgggtgtgg tgaatgtact gttatatgta
      721 tttataatgc aataccggta gtatggaaaa cagatgcaac tgctttattt gaacgtacag
      781 cagagtatgt agatgcgttt atgcaacaaa agaaaaataa agttgcattt ttcaatttta
      841 ttatgaatgt gtctcccgaa tgtgattgtt gctactggaa tgatacgcct attgtaccta
      901 atataggcat tttagcgtca actgacccta tagctatcga tagggcatcg gtagacctta
      961 ttaataaagc tatgccattg ccaggatccc gtatagcttt aaaagatgca tctaaggata
     1021 atattacagc actttatccg gtgcaatggg aatatctatt caagtatgca gcctcccttg
     1081 gggctggtga tgaaaagtat gaattgattg aaatttagac atttttaaca gcttgtatta
     1141 tgtttgtaac gaagaggtaa tcatacatga agagtccaca tgattttcct gcaattaatc
     1201 caaaaaaacc tgatggcata aagccaaatg gcatacccta taaggtgatg gtagttgaag
     1261 ataaagaatt tcatcgtaag caaataattc agattttaga atccgaaggg tatgaggtta
     1321 tagctgctgc cactaatggt gcagaagcat taaaattata tgattcgctt gcagctgatc
     1381 ttgaccttat tacaactgat ctggatatgc caaagctaga tggctatgct ttactgtatg
     1441 agttgagtca gaagaaaccc aaggcaaaga ttgtgtttat cagtgatgaa actacaaaag
     1501 gtgtgcttgc tgatcttctt aaaatgggag cagctgattt tatattgaag ccaattcaac
     1561 gaggaactat tcttgaacgc gttaaggcag tattgcaaaa aaaataatct tatatcataa
     1621 aggttaaagc tcatgggaga aaatgagaag gcatatcagc acctttcgga acaaacgaaa
     1681 aagataaata tagcactgca ttatacccat ggggatatgg aaaaggctcg tcaaatggta
     1741 gcgggcaact ataacgattt gtatgctatt aaagtgcgat ttgcttcttc tactatgttt
     1801 ggtgcgtttt tgcttttttt taatcatata tataaaatta tttcaggcct gacgcttatt
     1861 ctatcgccgt catatacgtt acaaaatatt aatccggaat gggactggat agtttttgaa
     1921 aaagagatag cagagctcat gcaaaaaggt gactatgacc agattttagc aaaaaccttt
     1981 cgtgacaaaa taatgaaaag ccttgatatt atactgttaa atgaattagc ccgcaatatt
     2041 gaattaaaaa atgagatagc aattgataac cttttcagaa agctcataca ggaaacactg
     2101 gcattgaaaa gggttgattc atttgttgta tatcagccaa tgtcatctct tgctatggaa
     2161 ttacattcag ttacaattcc taaattaaaa agtgaaattg ttgatggtga acaagtaaca
     2221 aagccagaaa cacagtcaac tgcaactggt gaagctgagt atgtcccaca ggttggtgta
     2281 gatggagtta agctaattat taaatcagct ttaatattat cacctattaa gggcaaacac
     2341 atctcaaaac tgtcacctgg cgatagagtc atgatatcca ttattgatac caatccacag
     2401 gcggtaaaag tggcacaggc tttaaacgct tatgaaaatg aaacatttaa accaattcct
     2461 gcccgtatca cgtatgttaa atacattcct caaaagggct ataaagtata tggtttaatc
     2521 gctaaaggta ttgttgttga tatcgatgag gaagaagaaa acattaaagt agcgcttgac
     2581 cctgcttatg ctgctaccat tcagtccccg gaagaagagg taaaacctaa ttatgtgctc
     2641 atagccattc ttttgctggc atttggtatt ttaatgggta ttctgtattt gttgctatgg
     2701 tcaaaataga ttagacagga cgtaccggaa taccgcgcat ggctaaatac tcttttgcct
     2761 gcttaatgct gtattctctg tagtgaaata ttgaggcggc taacactgca tcggcttttc
     2821 caattgcaaa tccctcatat aaatgtttta gggtaccaac accgcctgag gctatcacgg
     2881 gaatggtaac tgattcagct acttttttag taagttcaag atcatagcca agtttagttc
     2941 catcacggtc catgctggta agtaaaattt caccagcacc taaatcttca acttgctttg
     3001 cccattgtac tgcatcaata tcagttttag ttcttccacc gtgaagatat acattccatc
     3061 cgccgttatt gcgtttagcg tcaattgcaa ccacaatgca ttgtgaacca aaacgccgtg
     3121 cacattcggt gataagctct ggttgctgta ctgcctgtgt attgatagac accttgtcgg
     3181 caccattttc cagaatcatt cttacatcat caacagttct gatgccacct ccaacagtaa
     3241 aagggatatt cactgtttct gcaacttcct ttaccatctt taaaataatg gcgcgcctat
     3301 cgcttgatgc ggtaatgtca agaaatacca gttcatcggc tccttcttca tcataaaaca
     3361 tgccgttgtg aacgggatca cctgcatcct gcaggtttac aaaatttaca cctttgacta
     3421 cacgcccatc tttaacgtcc aggcagggga taattcgttt ggctaacatt ctacggtatc
     3481 ctgaaaaata aattgtacag ttatatggta tagatgcaat tggtatatgt caaatagtta
     3541 aaactggttt gacaatcaca actcaatcta tcaaatggaa ttgttatgtc aaaaaaaaat
     3601 gaatacatat catttgctga attttcgcgc acctacgtgc ctgccatcaa tgagtacata
     3661 tatacgtttt attccaataa ggaactatcg gcacataatc cagatataaa actggcttac
     3721 aatttgctta aagaatattg tacacgtgat ggcaagcgca tacgcccgct tttattatta
     3781 ttgtcatacc tgggttatgg tggccctatc tccgaaatac aatcagttat tccagtggca
     3841 gcctcacttg agtgcatgca cgcattttta ttaatccagg atgacatcat tgatggagcc
     3901 ataatgcgcc gtggcaaacc agcatttcat aaggtgtgcg atagttctta taataagtac
     3961 aacgaacata ttggttcatg ggtggcaata gtactggctg atgttctcat gagcaacgca
     4021 ctggagatga tttccggatt aaccacaacg tctatgtctg cgtttttgca tgaatttgcg
     4081 ataatatatg aacgaacagc actggggcag atgcttgata ttatatggtc taatccaaaa
     4141 tatatcgatg atgaaaatat cccacacgca attgcctcaa tgaaaacagc acattataca
     4201 gtttcaggcc ccttgcggct gggctacata cttgccgggg gtagcaacaa tgatgagttg
     4261 caaaaattgg agcaggctgg ttataaagta ggctttgcat tccagatgcg tgacgatatt
     4321 attggtgtat ttggcgttga ggaggatagc ggtaaatcag ccagttcgga tattgaagaa
     4381 ggtaaatata ctgtgctggt gcaaaagacc ttgcagcttt tacatgacag tactgttaaa
     4441 gagcagttta tggccatatt taaaaagaag caaaaaagta aagaggatat tcaggccatt
     4501 aaagcgtata tagcaaaaag tggtgccaaa gcaaatgttc agcaactgat ttcccggtat
     4561 gttaacaacg caaaagaaat tataccacag ttacaatgtg attcatttat gaagcgttgc
     4621 ttgttggatt ttattgaaag tttgtttaat tgaaaccttt aagataataa aaataattga
     4681 caaatttaat taatcatatt aaatttgtcc atatgatata taaaaatcgc cagctggaat
     4741 acattctaaa agaagctagt accacatttc ctgcattact ccttactggt ccacgacaat
     4801 cagggaaaac aacgctgctt aaaaatttat tttcagataa agcaaaattt atatcattag
     4861 atgaatccga catacgttta tgggcacatg aagatccccg agattttatt caaaacaata
     4921 tgccacctgt aatcattgat gaaattcaat atgctcccga tttgcttacc tatataaaac
     4981 gaagaattga tgatgaccga tcccctgggc agtggttctt aacaggttcc agccaattca
     5041 cactgatgaa gcatgtttca gaatcgttag ctggccgaac ggctattttt catctgctac
     5101 ctttttcgca ttcagaaata catggtagag tatatcctaa ttgggatgat tggtttacgt
     5161 caattaaaga taatgtcccc tgtgatgaag atttaggtaa tgtgttactc agaggaacat
     5221 atcctgagtt agtatgtaat gctaccataa atcgtaaaat atggtatgat tcttatattc
     5281 agacatatat tgaaagagat ttgtccctgg tttacaatat tggggatatt aatacgttta
     5341 ctaaaa
//
LOCUS       DAOSZP010000076         5315 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_12961_length_5315_cov_3.326046, whole genome shotgun sequence.
ACCESSION   DAOSZP010000076 DAOSZP010000000
VERSION     DAOSZP010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5315
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12961_length_5315_cov_3.326046"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            195..2135
                     /locus_tag="PK348_02620"
     CDS             195..2135
                     /locus_tag="PK348_02620"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase dimerization domain-containing
                     protein"
                     /protein_id="HXK65139.1"
                     /translation="MKITLLSISALIFICTACSKTPESVEVSEYTKSLIHNVSLKFDE
                     IDKQESTRGLIAEAKSLTINDNKGKVVWDMSAYSFLKKEKPQTAHPLLWEMAQLNATP
                     GLYQVVQGIYQIRGFDIANMAIIEGKTGWIVVDTLTTKETAMAAFDFFKKNVKKKPIV
                     AIIFTHSHIDHFGGVLGIITENDVKNKKITVIAPKGFMEEAFSENILLANAMARRAEY
                     QFGQDLPISPTGQIDSGIGKTAAQGSYSIVEPTVTVDTTPTKMIIDGVEFIFQYTPDT
                     EAPAEMTFYLPAYKAFCPAEIASRTMHNLYTLRGTKIRDAYKWSKYINESLQLFPDVE
                     VCFFTHNWPMYGNNRIREFLIKQADMYKFIHDQTIRLANKGFTPEEIAHKLTLPQSLS
                     LFMPNRQFYGVLQQNVKAVYQMYMGWYNGNPAYLNQLPDIERAKRYVEYMGGVKSILE
                     KAGKSYNNGDYQWVAEVLNHVVMAQPDNQEARKLLAKTYTQLAYQAESGVWRNVYLKA
                     AQELSTGMQKKKSVMFKGYDMLKHMPPEKLFDSMAVNLNPEKAEHSSISIAIHFSDKN
                     ASYMLYIRNSVLYYSKGIIGKQEADLHIPYTTFIKLITGAIDKTKILFDKDVQWNGSN
                     VALIKFFSMFEKQERFAIVTPR"
     gene            2231..3778
                     /locus_tag="PK348_02625"
     CDS             2231..3778
                     /locus_tag="PK348_02625"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HXK65140.1"
                     /translation="MTGFSQWFFSDYEHSDYLTKRKAFLVFIFALAVIVLLNSGVILS
                     FIFISVERGVGFLQSAIPASIFSLITMYYIKKGRLQLASNILVIFCSLVVLIGLFTKA
                     AHVGFVTMVYFMFATMLFASAFSTRVIASIIYGTYIVGLFVFYFLHADQVEGKLAEAL
                     KIGLIDSAAALTLSYLIVMMTITSFNRVIESLDIEKNKTIQQIGVLKSLHTIIEDISN
                     KMMSISSLISDKLKDFLQNIQDQASAVEEITASTQEVSNGFGNVNQNVNKQYNALKTL
                     FDTINALAQEIDLLKSRSVEISHAFESILTITRKGEDAIRIVNDNAKALLDSSGKLTS
                     IMNILQDIFDKIQLLALNASIEAARAGEAGRGFAVVADEVNKLSEQSVMSLKEINVNI
                     QSNIHSVETSNTGVATIVNLFQDIVSTLNTVSAGMQDIFKHIDNQEKIKEEIQTQVAG
                     SQTLSEQIAEDTNRHNEIIIEISNSIANINTLIQNNMSVAEDISDTSQELSNMGKDLL
                     NKIKEEA"
     gene            complement(3781..5076)
                     /locus_tag="PK348_02630"
     CDS             complement(3781..5076)
                     /locus_tag="PK348_02630"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HXK65141.1"
                     /translation="MSVLQSAMAAKEASIYLAAVSTSVKNKALQIIADEIKKNVEAII
                     QANTNDIQRSQEEHLPAPLLKRLKFDQAKINEVIAGIESLIALPDPVGNTLLATELDN
                     ALTLYKVSCPIGVIGVIFESRPDALVQISTLCLKSGNAVLLKGGSEAKETNKILAEII
                     YNAGVRAGIPEHWLTLLETREDVTALLKMDDYIDLIIPRGSNAFVKYIMDNSRIPVLG
                     HADGICHCYVDKDADLNMAVTVVTDSKVQYVAVCNATETLLVHRAIAHTFLPLIEQEL
                     KKYNVEIVGCEETQKIISVNPATEEDWKTEYLDYKLSIKVVDSVEDAIRHINKYGSKH
                     TDSIITSNKDTAKKFLDLVDSGNVFWNCSTRFSDGFRYGFGAEVGISTSKIHARGPVG
                     LEGLVIYKYKLIGNGNIVEDYAKGKRTFTHKKLNTQCPL"
BASE COUNT         1670 a          975 c         1057 g         1613 t
ORIGIN      
        1 gttctgctac cgcttcagtt tgaaacttta tatattttta ttttatctcc atgctatttt
       61 ctctatgaat aaaagtttca cattatacta aataaaaaat tctttcaata atcaatacat
      121 tagtctatac catggtgagg aacaattaat atgtatatgg gaaatactac tttatcaaga
      181 ggggagccag caacatgaaa attacattgt tatccattag tgcattaata ttcatttgta
      241 cagcatgtag taaaacacct gaatcagtag aggtttctga atatacaaag agcttaatcc
      301 acaatgtgtc gttgaagttt gatgaaattg ataaacagga atcaacacgt ggtttaattg
      361 ctgaagcaaa aagcctgacc ataaacgata acaaaggcaa ggttgtgtgg gatatgtcag
      421 catattcgtt tttaaaaaaa gaaaaaccac aaacggcaca tccattacta tgggaaatgg
      481 cacaattgaa tgcaacacca ggattatacc aggttgtaca aggtatatat caaataaggg
      541 gctttgacat tgcaaatatg gccataattg aagggaaaac aggctggatt gttgtggata
      601 cacttaccac aaaagaaact gcaatggctg catttgattt ttttaaaaag aatgtgaaga
      661 aaaagcccat tgtagcaatc atttttaccc atagccatat tgaccatttt ggtggtgtat
      721 tggggataat tactgaaaat gacgtaaaaa ataaaaaaat aacggtgata gcacctaaag
      781 ggtttatgga agaagcattc agtgaaaata tactccttgc caatgcaatg gcacgaagag
      841 ctgagtatca gtttggacag gatttgccca tttcgcccac agggcagata gatagtggta
      901 ttggaaaaac tgcggcacag ggttcctaca gtattgtgga gccgacagtt actgttgata
      961 caactcctac gaaaatgata atcgatggtg tggaatttat attccagtat acacccgata
     1021 ccgaggctcc agcagagatg acgttttacc tacctgcgta taaagccttt tgcccagctg
     1081 agattgcaag tcgcaccatg cataatctgt atacgttacg cggaacaaaa attcgggatg
     1141 catataagtg gagtaaatac attaacgagt cactacagct ttttcctgat gttgaagtgt
     1201 gcttttttac acataactgg cccatgtatg gcaacaatcg cataagggaa ttccttataa
     1261 agcaggctga catgtataaa tttattcatg accagaccat ccggcttgca aacaaaggat
     1321 ttacacctga agagatagct cataaactaa cgcttccaca gtcactatcg ctctttatgc
     1381 ctaaccgaca gttttacgga gtactgcaac aaaacgttaa ggctgtgtac cagatgtata
     1441 tgggatggta taacggtaat cctgcatatc tcaatcaact gcccgatata gaaagggcta
     1501 aacggtatgt agaatatatg ggtggggtca aaagcatact tgaaaaagca gggaaatcat
     1561 ataacaacgg tgactaccag tgggttgctg aggtgttaaa tcatgtggta atggcacagc
     1621 ccgacaatca ggaagcacgg aagctccttg ccaaaacata cacgcaactg gcttatcagg
     1681 ctgaatctgg tgtatggcgc aatgtgtatt taaaagctgc acaggaatta agcactggca
     1741 tgcaaaagaa aaaatcggtg atgtttaagg gatatgatat gctgaaacac atgccaccgg
     1801 aaaaattatt tgatagcatg gcggtaaact taaatcctga aaaggcagaa catagcagca
     1861 taagcattgc aattcatttt agcgataaga atgcgtcata tatgctgtat attcgcaatt
     1921 cagtgttgta ttattctaaa ggaataattg gcaagcaaga ggctgacctg cacataccct
     1981 atactacttt cattaagctt attaccggag ctatcgacaa aacaaaaata ctttttgata
     2041 aggatgttca atggaatggc agtaacgtag cactgataaa atttttttct atgtttgaga
     2101 aacaggaacg ctttgcaata gtaacaccac gctaattagt atggtaacga taaatagtta
     2161 ttgactatat atgtatgttt agcttactgt caatagaaac agataaatgt tatatagttg
     2221 aggtgttatc atgactggat tttcccaatg gtttttttct gattatgaac acagtgatta
     2281 ccttacgaaa cgaaaagcat tcctggtatt tatatttgcg ctggcagtta ttgtattgct
     2341 caattcaggt gttatactat catttatttt tatcagtgtt gaacgtgggg taggtttttt
     2401 gcagtctgct atcccggcat caatcttttc tttaatcacc atgtattata ttaagaaagg
     2461 gagattgcaa ctggcctcca atattctggt tattttttgc tctctggtgg tacttatagg
     2521 tctttttaca aaagctgctc acgttggatt tgttacaatg gtgtatttta tgtttgccac
     2581 catgctattt gcttctgcat tttcaaccag ggttattgct tcaatcattt atggcacata
     2641 tatagtggga ttattcgtgt tttacttttt acatgctgac caggttgaag ggaaactggc
     2701 tgaagcattg aagattggac tgattgatag cgctgcagcg ttgacgcttt catatttgat
     2761 tgttatgatg accattacct catttaacag ggttattgaa tcgctggata ttgaaaaaaa
     2821 taaaaccatt caacagattg gtgtgctgaa aagcttgcat accattattg aagatatttc
     2881 caacaaaatg atgtcaatat cttcacttat ttctgataaa ttgaaggatt ttttgcaaaa
     2941 tattcaggac caggcttctg ctgttgagga gatcactgca tcaacgcagg aggtgtcgaa
     3001 cggttttggc aatgtaaacc agaatgtcaa caaacagtat aatgcattga aaacactctt
     3061 tgatactatt aatgccctgg cgcaggaaat tgatttatta aaaagcaggt cggttgaaat
     3121 atcccatgcg tttgaatcaa tactgactat caccagaaaa ggggaagatg ctataaggat
     3181 agtaaatgac aatgctaaag cattgttaga tagctctggc aaattaacct caattatgaa
     3241 cattttacag gatatatttg acaagataca gctattggca cttaacgcct caattgaggc
     3301 tgcccgtgct ggcgaagccg gaaggggctt tgcggtggtt gccgatgagg ttaataaact
     3361 ttctgagcaa tcagttatga gtttaaaaga aattaatgtc aatattcagt ccaatattca
     3421 cagtgtggaa acttctaata ccggtgttgc aacaattgta aatttgtttc aggatatcgt
     3481 atccacatta aatacagtca gtgctggcat gcaggacata ttcaagcata tagacaatca
     3541 ggaaaaaata aaagaagaaa tacaaactca ggttgctggc tcacagactc tttcagaaca
     3601 gattgctgaa gataccaatc gccacaatga aatcattatt gagatttcaa attcaatagc
     3661 aaacatcaat acactcattc agaataatat gtctgttgct gaagatatca gcgacacatc
     3721 acaggaacta tcgaacatgg gtaaggattt gctcaataaa attaaagaag aggcgtagta
     3781 ttacaacggg cactgtgtat tcagtttttt atgcgtaaac gtacgcttgc cttttgcata
     3841 gtcttctaca atgttgccat ttccaattag tttatattta taaattacca gaccttctaa
     3901 cccaaccggt ccacgtgcgt gaatttttga agtgctgatc cctacttctg cgccaaagcc
     3961 atagcgaaat ccatcgctaa aacgggttga acagttccag aagacattgc ctgaatcaac
     4021 cagatcaaga aatttctttg ctgtgtcctt gttgctggta ataatgctgt cggtatgttt
     4081 tgaaccatat ttattgatat gcctgattgc gtcttcaaca ctatcaacaa ccttaataga
     4141 aagtttataa tccaggtatt ctgttttcca gtcttcttcg gttgcaggat ttacggaaat
     4201 aatcttttga gtttcctcac atccaactat ctccacatta tattttttaa gttcctgttc
     4261 aattaacggc aaaaaagtat gtgcgatagc tctgtgtacc agcaatgttt cagtagcatt
     4321 acacactgca acatactgta ctttggaatc agtaacaaca gttactgcca tgttcaggtc
     4381 tgcatcttta tcaacgtagc agtggcaaat gccatccgca tggccaagca ccggtatgcg
     4441 tgagttatcc atgatatatt tgacgaaagc atttgacccg cggggaataa taaggtcaat
     4501 gtaatcgtcc atctttaaaa gcgctgttac atcttcacgc gtttcaagca gtgtcagcca
     4561 gtgttccggt ataccagctc tgacacctgc attataaatt atttctgcaa gaattttgtt
     4621 ggtttctttt gcttcgctgc cacctttaag gagtaccgcg ttgccacttt tcaaacacag
     4681 tgttgatatc tgcaccagag catcaggccg tgattcaaaa attacaccaa tcacaccaat
     4741 aggacagctt actttataga gggtcagagc attatcaagc tctgttgcaa gaagggtatt
     4801 ccccacagga tcaggtaatg caatgaggct ttcaattcct gcaataactt cattaatctt
     4861 ggcttgatca aatttcaagc gtttgagcaa tggtgcgggc aggtgttctt cctggctgcg
     4921 ctgtatatca ttggtattag cctgtataat tgcttccacg ttttttttga tttcatcagc
     4981 tattatctgt aatgccttat tcttaacgct ggttgaaaca gctgccagat aaatggatgc
     5041 ttcctttgca gccattgcag attgtaatac gctcatggga tgacctcttc tggtataatt
     5101 atagtgttat tgggtaaaag ttttggtatt tgtgtcaata tatttttact ggtgtcagag
     5161 caaaagctca ccacggcaat aagcacacag acgttcaata cactcttcca atgtgttacc
     5221 taactctaaa aaatattgat gcagggtaat ctttatagtg gggcgatagt tcctgtcaaa
     5281 gtaagccctt gacccaccaa aatagctttc aaaag
//
LOCUS       DAOSZP010000077         5311 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_12969_length_5311_cov_3.193113, whole genome shotgun sequence.
ACCESSION   DAOSZP010000077 DAOSZP010000000
VERSION     DAOSZP010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5311
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12969_length_5311_cov_3.193113"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            56..2920
                     /locus_tag="PK348_02635"
     CDS             56..2920
                     /locus_tag="PK348_02635"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HXK65142.1"
                     /translation="MSKKSIHIIVTILVCVAAPLLIYPFGKNKVTIETFKWHILKTIH
                     CNVYYPYGMEQLATKTAVIAEKAYVHIADALQHELNDPVPIIVFPSHIDFQENNILLQ
                     IIGEGTGGFTEAFKNRVVVPFNGSYDEFQHVLVHELVHAFQFNILMRDSSGKMQSMMS
                     MGSVPLWFTEGMSEYLSIGFDETADMTMRDFFLNDQYISLMDFTTYRTGNMYYYYKVG
                     QAFFYYFEQVYGKGKIGEFLRDIRDLDNFEEALKVHTKKNLEEIDTEFKHFYKKRFYP
                     VIAGRNFDEEEGQRITNHQKTRSIFNTCPAISPDGKKIAYIDNRDIYSSISIITIGEK
                     EAEEKARVILKGNETSEFEGMHLLDNYLSWTPDGKGIVFVSQSKNRDVIFIIDASNGK
                     ILNEIVLPFRSIKDPALSPDGKFICFIGQTNIQSDVYIYSLHDKSLKQVTQDVYSERY
                     PKLAANNTVIFYSSNYPAGDYTSQQYAIIKHTLSTNAREVIVQPQSKNLQVDIAKDGN
                     SIVYISNREGIYNVYQYDISKYSETKITNASTGIFYPRIFPDANKIAFVSYQNGGYDI
                     FIKEDIKPVTIAPPFNTEHIPVMLPQSYFPLSQAIWDNYTTTVSPDWVFFGLGGTVGY
                     GFAGFAQMSFSDYLGDNRIVVTTDYLRYGSSANYLNWDVQYLYLRYRWDYSLGFFRQK
                     NPFGIFTLASINDLIHNAYWSTLSMDHYGVYAIASYPFTKFSRFSTRVSSSRYERDYS
                     YLDQRPDVFANLNSVSVSYNYDNVLWGFLAPLRGTRGQIMFTQVVNLTGQDYSFSSID
                     IDVRHYLFLDKQYVLAFRGIGGKIMGEKKEYFKYYIGGFNTLRGHPFIEYGGTNVFLF
                     NAEFRFTFIESIRMGWPLFLKIGNIGGVLFVDAGSAWDNTYTFMNKDTGEFQDFKMDM
                     GFGFRLTLYPIVILKLDYAWPYYYSGFGHKEIVFSLGYEF"
     gene            2938..3522
                     /gene="hisB"
                     /locus_tag="PK348_02640"
     CDS             2938..3522
                     /gene="hisB"
                     /locus_tag="PK348_02640"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004697683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HXK65143.1"
                     /translation="MERSAHIERKTKETDITVKLLLDSIEPGTIQTGIGFFNHMLTLF
                     AAHGRMSIQLNAKGDLDVDAHHTIEDTGICLGKAFQKALGDKQGIVRFGQAIVPMDEA
                     CSSVIVDISGRGYFVYRGDKLTGLIQTYSEELTLEFFKAFAHNAQITMHIQQLYGDNR
                     HHIHESIFKAAGIALYRAYTISDSTGIPSTKGVI"
     gene            3519..4127
                     /gene="hisH"
                     /locus_tag="PK348_02645"
     CDS             3519..4127
                     /gene="hisH"
                     /locus_tag="PK348_02645"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013841338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HXK65144.1"
                     /translation="MITVIDYGMGNLRSVVKAVEKYTSDVQISSDPGSIATSKALIMP
                     GDGAFGHAMENLTTLGWVEPLVDFIRGGGYFFGICLGYQLLFSTSEEFGHHKGLDIIP
                     GNVVRFTNDLKVPHMGWNQVVFKQHHPVVDGIPQYSYFYFIHTYYPVVLDSSWIIGQT
                     QYSVEFASIVGKGNCIATQFHPEKSHKYGLRMIENFVRLACS"
     gene            4118..4837
                     /gene="hisA"
                     /locus_tag="PK348_02650"
     CDS             4118..4837
                     /gene="hisA"
                     /locus_tag="PK348_02650"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HXK65145.1"
                     /translation="MLVIPAIDLKEGRCVRLVQGDPLQETVYSYDPESIAKQFEEAGA
                     KLIHVVDLDGAFEGQPVNMHIVKRIAKAVSIPIEIGGGIRTETAIKMYLDEGIERIIM
                     GTVLMEDTFREIAERFKEYIIAGVDAKDLKIATHGWKKIIDMDAIKCIKELQSIGISE
                     IIYTDIATDGMLQGPNYTSIEKILSEIPGIALIASGGISAIEDLEKLKKYENHGLKGC
                     IVGKAIYDGRIDLRNAITLCD"
     gene            4850..>5311
                     /locus_tag="PK348_02655"
     CDS             4850..>5311
                     /locus_tag="PK348_02655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HXK65146.1"
                     /translation="MNDDMSLYDEDIKFPIEIEQYERKIYDLKQLIEISKGLNSTLDY
                     NVLIDSILLTCMGQMQLLKAGIFTHRSIDRQEFVLHRNYKGFEIDHKNEYLIPTNSKL
                     IQFLDANFRCYTLNELKELLPDEPSLENLIRLDPSLIVPLKGKGTLNGIIVL"
BASE COUNT         1673 a          878 c         1128 g         1632 t
ORIGIN      
        1 tatatgtatt atctattaca ttctattttt tagtggagat ttttatctta ttctcatgag
       61 caaaaaaagt atacatataa ttgtcactat tctggtatgt gtagccgccc cattacttat
      121 ttatcctttt gggaaaaaca aggttaccat tgaaacgttt aaatggcata tattaaaaac
      181 aatccattgt aatgtgtatt atccctatgg gatggagcag cttgctacaa agaccgctgt
      241 tatagctgaa aaggcttatg tccacatagc tgatgcattg caacatgaac tcaatgatcc
      301 ggtgccaatc attgtttttc cctcacatat tgattttcag gaaaataata ttcttcttca
      361 aatcattggt gaaggtacag gaggatttac tgaagctttt aaaaatagag ttgttgtgcc
      421 tttcaacggt tcgtatgatg aatttcagca tgtccttgtt catgaactgg tccatgcatt
      481 tcagtttaac attcttatgc gcgatagttc cggtaaaatg caatcaatga tgagcatggg
      541 ttctgtaccg ctatggttta ctgaaggcat gtcagagtat ttatcaattg gctttgatga
      601 aactgcagac atgaccatgc gtgatttctt tttaaatgat cagtacatta gtttaatgga
      661 tttcacaacg tatcgaactg gcaatatgta ctattactat aaggttggac aggctttttt
      721 ttattacttt gagcaagtat atggaaaagg taaaattggt gaatttttac gagatatacg
      781 ggatctggat aactttgaag aagcattgaa agtgcacaca aagaaaaacc ttgaagaaat
      841 tgatactgaa tttaaacatt tttataaaaa gcgcttctat cccgtaatag caggaagaaa
      901 ttttgatgag gaagaaggcc agcgaataac caaccaccag aaaacgcgtt caatatttaa
      961 tacctgtcca gctatctcac ccgatggaaa aaaaattgcc tatattgata acagggatat
     1021 ctattcctcc atttccataa taactattgg tgaaaaggaa gctgaagaaa aagcgcgtgt
     1081 catactgaag ggcaatgaaa catctgaatt tgaagggatg catttgctgg ataactatct
     1141 gtcatggacg cctgatggta aaggtattgt gtttgtatct cagtcaaaaa acagggatgt
     1201 tatatttatt attgatgcca gtaatggaaa aattttgaat gaaattgtat tgccattcag
     1261 atcaataaag gaccctgcgt tatcacctga tggaaagttt atatgtttca ttgggcaaac
     1321 aaatatacag agtgatgtgt atatctattc attacacgat aaatcgttaa aacaggtcac
     1381 gcaagatgta tacagtgagc ggtatccaaa attagctgcc aataatactg tcatctttta
     1441 ttcatcaaat tacccggcag gggattatac cagccagcaa tacgccatca ttaaacatac
     1501 actatcaaca aatgcccgag aagttattgt acaaccacaa tcaaaaaatt tacaggttga
     1561 tattgcaaag gatggaaata gtattgtata tatttcaaac agggaaggta tttacaatgt
     1621 ttatcagtat gatatatcca aatatagcga aacaaagata accaatgcat caaccggtat
     1681 attttatccg cgcatatttc ctgatgcaaa caaaattgca tttgtatcct atcagaatgg
     1741 cgggtatgat attttcataa aagaagacat taaaccagta actatcgccc ccccttttaa
     1801 tactgagcat ataccagtca tgttgccaca aagctatttc ccattatcac aggctatatg
     1861 ggacaattac acaacgactg tttcacctga ctgggtgttt tttgggcttg gtggcactgt
     1921 gggatatggt tttgctggtt ttgctcagat gagtttcagc gattatttag gcgacaatcg
     1981 catagtggta accacggatt atcttcgcta tggcagcagt gctaattatt taaactggga
     2041 tgtgcagtac ctttatctgc gctatcgatg ggattattcg ttaggttttt tcaggcaaaa
     2101 aaatcctttt ggcatattta ctctggcaag tataaatgac ctgattcaca atgcctactg
     2161 gtccactctg tcaatggatc attatggtgt atatgccatt gcaagctacc catttaccaa
     2221 gttttcccgg ttcagcacgc gtgtttcgtc aagccgctat gaacgcgatt acagctactt
     2281 agatcagcgc cccgatgttt ttgccaattt aaattcagtt tcggtgtcat ataactacga
     2341 taatgtattg tggggatttc tggctccctt acggggcaca cgcggccaga taatgtttac
     2401 ccaggttgta aatctgacag gccaggatta ttctttcagc agtattgata ttgatgtgcg
     2461 ccactatttg ttcctggaca aacagtatgt acttgcattc agaggaattg gcgggaaaat
     2521 aatgggtgaa aagaaagaat atttcaagta ctatattggt ggatttaata cgctgcgtgg
     2581 tcatccgttt atagaatatg gtggtaccaa tgttttcctg ttcaacgctg aattcaggtt
     2641 tacgttcatt gaatcaatac gaatgggttg gccattgttt ttaaaaattg gtaatattgg
     2701 tggcgtttta tttgttgatg ctggttcggc gtgggataat acgtatacat ttatgaataa
     2761 agatacgggg gaatttcagg attttaaaat ggatatgggt tttggattta ggcttacgct
     2821 atatcccata gttattttaa agcttgacta tgcctggccg tattattatt cagggtttgg
     2881 acataaagaa attgttttca gtttaggata tgagttttaa atacaggaga ataatcaatg
     2941 gaacggtctg cacatattga gcgtaaaacc aaagaaaccg atataaccgt aaaactttta
     3001 cttgatagca tagagccagg caccatacaa accggcattg gtttttttaa ccatatgctg
     3061 acactgtttg cagctcacgg aagaatgagt attcagttga atgcaaaagg tgatttagat
     3121 gttgatgctc atcatacaat tgaagataca ggaatttgcc ttgggaaggc attccagaaa
     3181 gcccttgggg ataaacaggg cattgtacgg tttggccagg cgatagtacc tatggatgaa
     3241 gcatgcagca gtgtaattgt cgacatatcg gggcgaggat attttgtata ccggggtgac
     3301 aagttaactg gtttgataca aacatatagc gaggagttga cactggagtt tttcaaagca
     3361 tttgcacaca atgcacaaat taccatgcat attcaacaat tatatggcga taacaggcac
     3421 catattcacg agtcaatatt caaagctgca ggcatagcac tgtaccgggc ctatacaata
     3481 agcgatagca ctggcattcc ttccacaaaa ggagtcatat gattaccgtt attgattatg
     3541 gtatgggaaa cctgcgctca gttgttaagg cagtggaaaa atataccagc gatgtccaaa
     3601 taagctctga ccctggcagt atagctactt ccaaagcact gataatgcct ggtgatggtg
     3661 catttgggca tgcaatggaa aatttaacta cgctagggtg ggttgagccg cttgttgatt
     3721 tcatacgggg tggaggatat ttttttggaa tatgcctggg gtatcagctt ttattcagta
     3781 ccagtgaaga atttggccac cataagggac ttgacataat acctggtaat gtggtacgat
     3841 ttactaatga tttaaaagta ccgcatatgg gatggaatca ggttgttttc aaacagcatc
     3901 acccggttgt tgacggtatt ccgcaatata gctactttta ttttattcac acatattatc
     3961 ctgtagtgct tgatagctca tggataatag gccagacaca gtattcagtg gaatttgcca
     4021 gtattgttgg gaaaggtaat tgcatagcaa cacaatttca tcctgaaaag agccataaat
     4081 atggactgcg catgatagaa aattttgtga ggttggcatg ctcgtaatac cagctattga
     4141 tttaaaagaa ggtcgatgtg ttcgactggt gcagggggac cctttacagg aaacagtgta
     4201 ttcatatgat cccgaaagta ttgcaaagca gtttgaagaa gcaggagcaa aattaattca
     4261 tgtagttgat cttgatgggg cttttgaagg gcagcctgtc aatatgcata tagtaaaaag
     4321 aatagcaaaa gcagtatcaa tccccattga aataggaggt ggtatcagga ctgaaactgc
     4381 aataaagatg tatctggatg aaggaattga aaggattatt atgggaacgg tgttaatgga
     4441 ggataccttc agggagatag cggagcgatt taaggaatat attattgctg gtgttgatgc
     4501 caaagatttg aaaatagcta ctcatggctg gaaaaaaatt attgacatgg atgcaatcaa
     4561 atgtataaag gagcttcaaa gtattggcat ctctgagatc atttatacag atatagcaac
     4621 tgatgggatg ctgcaggggc caaattatac gtccatagaa aaaattttat ctgaaattcc
     4681 tggcatagct cttattgctt ctggtggtat ttcagctatt gaagatttgg aaaagttaaa
     4741 aaaatatgaa aatcatggat taaaaggttg tattgttggt aaggccatat atgatgggag
     4801 gatagattta cgcaatgcta ttacattgtg tgactaaatg gtgaaacaca tgaacgatga
     4861 catgtcatta tatgatgaag atataaaatt ccccattgaa atagagcagt atgagcggaa
     4921 aatctatgat ttaaagcagt tgattgaaat aagcaaggga ttaaattcaa cgttagatta
     4981 taatgttctt attgattcaa ttctcttaac ctgtatgggt cagatgcaac ttctaaaggc
     5041 tggcatcttt acacacagaa gtattgaccg gcaggaattt gtattacaca ggaattacaa
     5101 aggctttgaa atagaccata aaaatgaata cttaattcct actaattcca aactcatcca
     5161 gtttcttgat gccaatttcc gttgttatac attaaatgaa ttaaaggagc ttctgcctga
     5221 tgagccttca ctggaaaatc tcatacgatt ggacccatca ttgatagtcc cgttaaaggg
     5281 caagggtact ctcaatggaa taattgttct t
//
LOCUS       DAOSZP010000078         5307 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_12980_length_5307_cov_3.232483, whole genome shotgun sequence.
ACCESSION   DAOSZP010000078 DAOSZP010000000
VERSION     DAOSZP010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5307
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12980_length_5307_cov_3.232483"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="PK348_02660"
     CDS             <1..246
                     /locus_tag="PK348_02660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65147.1"
                     /translation="YCFNSFTYEFYKNNSVSCFTISGDMDALDITTKYYNGYIQFKKP
                     RIYLVTRLRIPDAVYTFKHKQFCPQHYANYDVLIDCE"
     gene            complement(260..1798)
                     /locus_tag="PK348_02665"
     CDS             complement(260..1798)
                     /locus_tag="PK348_02665"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HXK65148.1"
                     /translation="MKVKSIADLYALYNPLIRRRAVLLYIMNIIILCLVLPVPFLVLI
                     IRGDFLRPFIIGIAPIIGSLISIITLRKGIYHFSANATSLATSIAILLGSYLQYQGTP
                     GIGYSSMIYIMPAGIVFASLFCSRLWTTGIATLFFISNIYFYYLNTLQGIIDKKILFT
                     GLIDSSLSIVFCFALSFLIVKIMKDSMNEIQIESDKNKEQYAQLKELIIAVQQVAANL
                     KDASAKMRDMTGRLSEQAQSQAASVEEVTSAIEEVNASMDLVNQHVKDQTVNLELLDS
                     MTSDFTELFGQLRQTIDTVARKIYTTADQSRNNEKTLEELNITMQEVGVKSQQMNSVA
                     DVIDTIAEQISLLALNASIEAARAGEAGRGFAVVADEISKLSDQTGESLKEIYNLTKE
                     TETQIQKGIKIVQETVQAMSAVIHNVHEISNDIQNISKNMAMQIENNKTISDKTKETH
                     QHADEIHYSASEQMTALGEVAKSIAMINDYTQDLSNAVVNLLEIAKIVDNEALQLKER
                     TQIL"
     gene            1924..2862
                     /locus_tag="PK348_02670"
     CDS             1924..2862
                     /locus_tag="PK348_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 and DUF1722 domain-containing protein"
                     /protein_id="HXK65149.1"
                     /translation="MQFPKPVICVSACLNGQNIRYNGQTINDDFVRLLLKYCKALDVC
                     PEVSIGLGVPRDHILVYYANNQFRLFQPATHKDVTGLMQQFATSFLSTLQGVDGFILK
                     AKSPSCGVSGTKIYKDTMGSIFHSKGKGLFATEVINKFGFLPIEDESRLRDEDIRENF
                     LIRIFTLARFREMLSNNVSIQKLIQFHQENKYIMMTYYQRKLSQLGRIVAGYKKGAFG
                     KTLNDYKTVFYTAFTRKPSQKQHVNTILHIFGYFSDKLNSNEKKHFLHLLDQYREHKI
                     SRIVIIELLRNWAHRFNDTYLLSQQYLDPYPEELKT"
     gene            complement(3001..>5307)
                     /locus_tag="PK348_02675"
     CDS             complement(3001..>5307)
                     /locus_tag="PK348_02675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HXK65150.1"
                     /translation="EKELRESEEKWRAITENLTDIVWIVNLQFETIYINKAVEKLFGF
                     TVDEYLQRKVQEKYPPEVLQDIYNKLIEEFENEKKPGIDKNRTRIVEIQEYKKDGTLA
                     DLSVHVKFLRDEAGNPNAIIGISRDITEQKKRLKEIQRYKLIFEQSINEIYIFDAKTL
                     MFTYVNQASINNLGYSLEELQRMTPVDIKPEFTLQQFNELLSPLREGTEKSIVFETVH
                     KRKDGSTYAVEVHLQLVKIDGEEHFAAIILDISERKRIEKSLKESEEKFRILAESTPM
                     TIMMYQDNKWIYANPAAEALLEYSEQELCSMNFWDVVHPDDKAIVIERGSKRQKGEDA
                     TVGYEFRVISKSGMIKWVLLYGATVMYKGKPAGLISVLDITERKKIEEEKRKLQEQLL
                     QSQKMESIGTLAGGVAHEFNNMLNIISGHAELALLNIPQENDARKDIEEIKKAAQRSA
                     EITKKLLTFARKQEEKSSIVNVNSAIDSMIGMLKRLIGENIQLVWQPANRELSVQIDE
                     VSIDQILVNLSVNARDAIDKQGTITISTHSVSIKDEVALQHNVLPGEYALIQVTDDGI
                     GIDEETQKLIFDPFFTTKEVGKGTGLGLSTVYGIVKNNGGFIEVQSEKEKGTTFKIYL
                     PVFIERRKFAQYHDNAVKDLFHGTETILIVEDEVSILKMIKKMLEILGYTVLEAGDPF
                     EGLEIVKNYIGTIHLLITDVVMPKMNGVDLAKLVSAIYPQIKILYTSGYSAEVLLKHN
                     IKASDINYLSKPVSFASLASTVRTILDN"
BASE COUNT         1607 a         1031 c          808 g         1861 t
ORIGIN      
        1 tactgtttta attcttttac gtatgaattt tacaaaaata attcagtatc ttgttttact
       61 atttcaggcg atatggatgc gctggatatt acaactaaat attataatgg atatattcaa
      121 tttaaaaaac cacggatata tctggttaca cgactacgta tacctgatgc ggtatataca
      181 ttcaaacata aacaattttg cccgcaacac tatgctaact atgatgtact tattgattgc
      241 gaatagcaca aaacacatgc tatagtatct gtgtcctttc tttcaactga agtgcctcgt
      301 tatcaacaat ttttgcaatt tcaagaagat taactacagc gtttgacaga tcctgagtat
      361 aatcattaat cattgcaata gattttgcaa cttcaccaag tgctgtcatc tgttcggaag
      421 cagaataatg tatttcatct gcatgctgat gtgtttcttt tgttttgtca cttatagttt
      481 tattattttc aatttgcatt gccatatttt tacttatgtt ttgaatatcg tttgatattt
      541 catgtacatt atggattaca gcactcattg cctgcactgt ttcctggaca atttttattc
      601 ccttttggat ctgtgtttcc gtttctttag tcaaattgta aatttccttt aagctttctc
      661 ctgtttgatc tgataatttt gatatctcat ccgcaaccac agcaaatcct cttcctgctt
      721 cacccgcacg cgcagcctca attgaagcat taagtgccag caggctaatc tgttctgcta
      781 tggtatcaat aacatctgct actgaattca tctgctgaga ttttacgcca acttcctgca
      841 ttgttatatt gagctcttcc agggtctttt cattattgcg cgattgatct gctgtagtat
      901 atattttacg ggctactgta tcgatagttt gccgtagctg gccaaacagt tcagtaaaat
      961 cacttgtcat ggaatctaaa agttcaaggt ttactgtttg atctttcaca tgctgattta
     1021 ccaaatccat actggcattg acttcttcaa tagctgatgt aacctcttct accgatgctg
     1081 cctggctttg agcctgttcg gatagcctgc ctgtcatatc acgcatttta gcagaagcat
     1141 cttttaaatt tgcagctacc tgttgaacag ctattataag ttcttttaac tgtgcatact
     1201 gttctttatt tttatcagat tcaatttgaa tttcattcat tgaatctttc attattttta
     1261 caatgagaaa tgacaatgca aaacaaaaaa ctattgaaag gctactatct attaaacctg
     1321 taaatagaat ctttttatca attattccct gcagtgtatt aagataataa aaatatatat
     1381 tacttataaa aaataatgtt gcaataccgg tagtccacag cctgctgcag aatagtgatg
     1441 caaatacaat acccgctggc attatatata tcatagaaga gtaacctata ccaggtgtac
     1501 cttgatattg cagatatgat cccaacaaaa tggcaattga agtagcaagc gatgtagcat
     1561 ttgctgagaa atggtatata cctttacgca aggttataat gcttattaat gatcctataa
     1621 ttggagcaat gcctataatg aatggcctta aaaaatcacc acgaataata agaaccagaa
     1681 atggaactgg cagaaccaaa caaagtataa ttatgttcat tatatataac agcacagctc
     1741 ttcgccgaat taatggatta tataaagcat ataaatcggc aatactttta actttcatac
     1801 tatcctctca ccaacagtgt ttgtgacaaa atatcataaa ctggatggac ggtcaaccaa
     1861 tttttttatt gacaaacata ttacatcatg taaactaaaa tcacttttca atggaggaat
     1921 cttgtgcaat ttccaaaacc tgttatatgc gtcagtgcat gccttaatgg ccaaaatatc
     1981 agatataatg gacaaacaat taatgatgat tttgtcaggc tgcttctgaa atactgcaaa
     2041 gcgttagatg tatgtccgga agtttcaatt ggtttgggtg taccaagaga ccatatcctg
     2101 gtctattatg ctaataatca attcaggctt tttcaaccag ctacacacaa ggatgtaaca
     2161 ggtctcatgc agcaatttgc aacatcattt ctttccactc ttcagggtgt tgatggtttt
     2221 atattgaagg caaaatcgcc atcgtgtggg gtatcaggaa caaaaattta taaagacacc
     2281 atgggtagca tttttcacag caagggcaag gggctttttg ctacagaagt aatcaataaa
     2341 tttggatttt tacctattga agatgaaagc aggctgcgtg atgaagatat aagagaaaat
     2401 ttcttaatca ggatcttcac cttagcacgt ttcagagaaa tgttatcaaa taatgtgtct
     2461 attcaaaaac ttatacaatt tcaccaggag aataaatata ttatgatgac atattatcaa
     2521 cgaaaactta gccagttagg aagaatagtt gctggatata aaaaaggtgc atttggaaaa
     2581 acgttgaatg attataaaac agtattttat actgctttca cgagaaagcc ctcacaaaaa
     2641 cagcatgtta atactatatt acacatattt ggatatttct ctgataaact caattcaaat
     2701 gaaaaaaaac attttctgca tttacttgat caataccgcg aacataaaat aagccgtata
     2761 gttattatag aactattaag gaattgggca catagattca atgatacata cttactatct
     2821 caacaatatc ttgatccata ccctgaagaa ttaaaaacat aaaattcttc aatacttaaa
     2881 agaacatgtt gccccataca aagtgccaaa gcttattgta tttatggatc agttacccac
     2941 cagtggtgtt ggtaaaatat tgaagcggga aatcaagaaa atgcttagtg aaatataatt
     3001 tcaattatca agaatcgttc tcacagtaga cgccaaactg gcaaatgaaa caggcttact
     3061 taaataatta atatcactgg ccttgatatt atgtttcagc agcacttcag cagaatatcc
     3121 tgaggtatat aatattttaa tctgaggata tattgctgaa acaagttttg ccaggtcaac
     3181 accattcatt ttgggcataa caacatcagt aataagaaga tgtattgtcc caatataatt
     3241 tttcacaatt tccaatcctt caaacgggtc acctgcttcc aatacggtgt atcctaaaat
     3301 ctctaacatc tttttaatca ttttaagaat tgaaacttca tcctcaacaa taagaattgt
     3361 ttcagttcca tgaaataggt cctttactgc attgtcatgg tattgtgcaa attttcttct
     3421 ttctatgaat acaggtaaat agattttaaa cgttgtacct ttctcttttt cgctttgcac
     3481 ttcaataaat cctccattgt tttttactat accatacacc gttgataatc ccagcccggt
     3541 ccccttgcca acttcttttg tggtaaaaaa agggtcaaag atgagtttct gtgtctcttc
     3601 atctatgcca ataccatcat cagtcacctg tattaatgca tactctcctg gtaaaacatt
     3661 gtgctgtaat gcaacttcat ctttaattga tacactatgt gtgcttattg taatggttcc
     3721 ctgtttgtca atagcatcac gtgcattaac actcagatta accagtatct gatcaatgct
     3781 cacttcatca atttgaaccg atagctccct gttcgcaggc tgccatacaa gctggatgtt
     3841 ttcaccaata agcctcttaa gcattcctat cattgaatca atagctgaat tgacatttac
     3901 tatagaggat ttttcttctt gctttcgtgc aaatgtcagc aatttttttg ttatctctgc
     3961 agacctctgc gctgcttttt ttatttcttc aatatcttta cgtgcatcgt tttcttgagg
     4021 tatatttaat aatgccagct ctgcatgccc cgaaatgata ttaagcatat tgttaaactc
     4081 atgcgctaca ccaccagcga gcgtaccaat tgactccatc ttctgtgatt gcaagagctg
     4141 ttcctgcaat ttcctttttt cctcttcaat tttttttctt tccgttatat ccaataccga
     4201 tatcaatcct gctggcttac ctttatacat tactgtggca ccatacaata aaacccattt
     4261 tatcatgcca cttttactaa ttactctgaa ttcatatcct actgttgcat cctcaccttt
     4321 ttgccttttg ctgcctcgct caatgacaat agctttatca tcaggatgga caacatccca
     4381 gaagttcata gaacacaact cctgttctga atattccaaa agtgcttctg ctgcaggatt
     4441 tgcataaatc cacttgttat cctgatacat cattatagtc atgggtgttg attctgccag
     4501 gatacggaat ttttcttcac tttctttaag gcttttttca atacgttttc gttcggatat
     4561 atcaaggatt attgctgcaa aatgttcttc gccatctatc ttcactaatt gcaaatgtac
     4621 ttccacagca tacgtactgc catcttttcg tttatgtacg gtttcaaaaa ctatactttt
     4681 ttcagtacct tctctgagtg gtgacaataa ttcattaaat tgctgtagag taaattcagg
     4741 ttttatatca acaggcgtca tacgttgtaa ctcttcaagg ctatagccta aattgttaat
     4801 tgacgcctga ttgacatagg taaacataag cgttttagca tcaaaaatat atatctcatt
     4861 aatggattgt tcaaaaatga gtttatagcg ttgaatttcc ttaagcctct ttttctgctc
     4921 tgttatatcc cgtgatatac ctattatggc atttggattg cccgcttcat ctcgtaaaaa
     4981 tttaacatga acgcttaaat cggcaagtgt tccatctttt ttatattcct gtatttcaac
     5041 aatcctggtt ctgtttttat caatccccgg ttttttttca ttttcaaatt cttcgataag
     5101 tttgttatat atatcctgca atacttcagg tggatatttt tcttgaacct tacgctgcag
     5161 gtactcatct actgtaaatc caaataattt ttccaccgct ttatttatat atatcgtttc
     5221 aaattgaaga ttaactatcc acacaatatc tgtaaggttt tcagtgatag ctcgccattt
     5281 ttcttcactt tcacgtaatt ctttttc
//
LOCUS       DAOSZP010000079         5287 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_13049_length_5287_cov_3.151758, whole genome shotgun sequence.
ACCESSION   DAOSZP010000079 DAOSZP010000000
VERSION     DAOSZP010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5287)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5287)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5287
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13049_length_5287_cov_3.151758"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            325..1833
                     /gene="amrB"
                     /locus_tag="PK348_02680"
     CDS             325..1833
                     /gene="amrB"
                     /locus_tag="PK348_02680"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HXK65151.1"
                     /translation="MNGIPVIAVLILLFSLQCTESYSITKQSKLAGSWYPSDKNELLK
                     YVSSSEHPSQQISHIPLIIVPHAGYIYSAHVAAKAYSAIASYKPDIIVIIGPSHYEYF
                     TGFALPVYDAIKTPLGVVTVEQKVIHTLSQNNTFTFNNNAFDPEHSIEIQLPFIQHYF
                     SGIPILPVLAGNVTIQQARQAAMQLLPAIAHYKKPLFVISSDFTHHGQRFSYAPYYKL
                     QPDKRMEDIKRDDMKAIDYILSKNTEKFYNYCQSTGITICGHNAIVLGLLLLSKVYTK
                     ELVAYSTSASITNDYDNTVSYAAVVITGEINTISTSTLGDSSCNISSEDKKFLLSLAR
                     QSIETMLKNKTLVEYKGTIPEHCKQNAGAFVTLTINGNLRGCIGYIDPVKPLYQTVIE
                     CAAHAAFEDPRFKPLTKDEYKKIHIEISVLSPYKPIASLDELRLGTDGLIVVKGNRRG
                     VLLPQVATEYCLTKQQFFQHTCEKAGIYPFEYDSVTMYTFTAAIFSENEIMK"
     gene            1894..3015
                     /gene="amrS"
                     /locus_tag="PK348_02685"
     CDS             1894..3015
                     /gene="amrS"
                     /locus_tag="PK348_02685"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HXK65152.1"
                     /translation="MRSVTRKQFLHSCLYSMQALVVGSVIPFLPEDVIASNYRQALHW
                     KKIDAQTVQCNLCPNQCMLTNGTRGLCKARQCMDGKLYSLVYGRIAAYHIDPIEKKPL
                     YHFLPGNDALSVATAGCNFSCKFCQNWQLSQSQPEKLDSQYITPVQLVKQAKEHKTPV
                     IAFTYNEPSIQYEYIYDTVQIAKKDNISCVIISNGYLNKHASEMLYPLLNAIKIDFKA
                     YSQKFYSEICGGNLSPVLSNIELAKKKNVWLELVNLVIPTLNDNPDDIGAMVRFIKTH
                     CGRTTPLHFTRFYPIYKLKNIPPTPVSTLERCREIALAEGLKYVYIGNVPGHSANNTY
                     CHNCGKPVIERSGFYTTKNKHKSGKCPYCNTAIPGIWTL"
     gene            3012..5192
                     /locus_tag="PK348_02690"
     CDS             3012..5192
                     /locus_tag="PK348_02690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65153.1"
                     /translation="MKYNRVFLYISTFFIGISGSIAQAIMIRELLMFFRGNELSIGII
                     MVLWFAGITAGAAISKKIKAHQFSSIAHNILLHPSSVCISLILLYCTPIFYHTYDSFY
                     PLEVELIIAVVCLLPQCFIIGYIFPVLVNACSRLTINNPGTVVFLLESLGAFTGGIAF
                     TFLIIQKINPLGTTLILFIIACIISFTVVQQRIKRLAIVIAMVIVAFVIAHSSYVEKK
                     LLSLSFAANHPGTLLEYRRTPYQMMFVAGVDDTIALYGNNNLYAQLPDDYQVRPIIHA
                     LLSTGSVLKDTDILISGDTAFLHYVFAYAGASIDHVTVDPVMWNIVQQKINSIYPDFP
                     TKLINVYYDDTRHYLKSTGKKYNAVIILPPPPDSIMHNRLYTVEFFKDCKLHLTHNGV
                     CIIQIEGFANIMNPFLRRYVASVINSFRQVFPSSLISAGDTIYCIGFNYNRDTDTSNY
                     LSQYLKNFSASPISKTIRILVPGFSPQEFKMYFQESQQEYLAKQLQSEHIINSDLLPK
                     GYFEYLANSLKQSSSEAERIITNPLLMILLLIIIVILSLWYFNSRGTQHIIFGSIIFV
                     SGFISMAAMIIALIAYQNVFGVLYYKIALCNALFMLGLAIGSASTFTQKDFFITRIMV
                     LITGLVMLFCFTIYNNAILLYVSIILIAATTGSFIPLLLNRKTNDIQLLASTLDAADH
                     SGALCGALITPFVLLPFFGIYSLLILIVMGFILIVFRLQNYSHI"
BASE COUNT         1702 a         1021 c          854 g         1710 t
ORIGIN      
        1 gccatcaagc cagttagttg agcacatgcc atttaaatgc aaaatatagg ttgtgctgaa
       61 accggctgaa gcaataaaca caaaacctgc cacgaaaagc aatactacag caaacctctt
      121 cataaacccc ccatatctat aatataatta taccatatag acggtatgta taaatcatcg
      181 aaatattatg tcaattttgt caagattttt ttttaaatat ttttcgataa cgatttatat
      241 attttatgaa tttatacttg attcttatac tattatttca tatttcaatt tgacaattaa
      301 aaaataatat tttattttat caacatgaat ggtataccag taatagcagt acttatatta
      361 cttttttctt tacaatgcac tgaaagctat agcattacca aacaatctaa attagcagga
      421 tcatggtatc cttccgataa aaatgaactt ttgaaatacg tttcatcatc agaacatcca
      481 tcacagcaaa tatcacacat tccactcata atagttcctc atgcaggcta tatctatagt
      541 gctcatgttg ctgcaaaagc ttacagtgct atagcaagct ataaacctga tattattgtc
      601 ataataggac catctcacta cgaatatttt actggatttg cccttcccgt ttatgatgct
      661 atcaaaactc ctttaggtgt tgttactgtt gaacaaaaag taatacacac attgtcacaa
      721 aacaatactt ttacgtttaa caacaatgcc tttgatcccg aacattctat cgaaattcaa
      781 ttgccgttta ttcagcatta tttctctggg atccccatcc ttcctgtgct tgcaggcaat
      841 gtaacaatac agcaagccag acaggcagct atgcaattat taccggcaat agcgcactat
      901 aaaaagccac tatttgtcat aagcagcgat tttacccacc atggccaacg atttagctat
      961 gctccctatt acaagctgca accagataag cgtatggaag atatcaaacg tgatgatatg
     1021 aaagcaattg attatatact ttcaaaaaac actgaaaaat tttataatta ctgccagtca
     1081 acaggcatta caatatgtgg tcataatgca atagtgttag ggctgttatt attgtcaaag
     1141 gtatatacaa aagaattagt agcgtattca acgtcagcca gcattactaa cgactacgat
     1201 aacacggtaa gctatgcagc tgtagtaata acaggtgaaa ttaataccat aagcacaagc
     1261 acattgggcg atagttcctg taatatatcc agtgaagata aaaaattttt gctttcatta
     1321 gctcgtcaat ccattgaaac catgctgaag aataaaacat tagtagaata taaaggaaca
     1381 atccctgaac actgtaaaca aaacgcaggt gcctttgtta cattaactat taatggtaat
     1441 cttaggggtt gtattggtta tattgatcca gtaaaacctc tttatcaaac cgttattgag
     1501 tgtgctgcac atgctgcttt tgaagatcca cgctttaaac cacttacaaa agatgaatat
     1561 aaaaaaatac atattgaaat atctgtgtta tcgccatata aaccaattgc atcactggat
     1621 gaattgcgtc tgggaactga tggccttatt gttgtaaaag gcaacaggcg gggtgtttta
     1681 cttccacagg tagcaacaga atattgttta acaaaacagc aattttttca gcacacgtgt
     1741 gaaaaggcag gaatttatcc atttgaatat gactctgtaa caatgtacac gtttactgct
     1801 gcaattttca gtgaaaatga aatcatgaaa taacatttta tgatgttttt aatgtaacat
     1861 tggttctatc caataagaat ctggattgta aatatgagat ctgttactcg taaacaattt
     1921 ttacatagct gtttatattc aatgcaagcg cttgtggttg gctcagttat tccattcctg
     1981 ccagaagatg ttattgccag taactatcgc caggctttac actggaaaaa aattgatgca
     2041 caaaccgtgc agtgtaacct gtgtcccaat caatgcatgc taacaaacgg cacaaggggt
     2101 ttatgcaaag cccgacagtg catggatggc aaactataca gcctggtata cggacgtatt
     2161 gctgcatatc atatagaccc aattgaaaaa aagccattgt atcatttttt gccaggcaat
     2221 gatgcattgt cagttgcaac cgctggctgt aattttagct gcaaattttg ccagaactgg
     2281 cagctttcac agtcacagcc tgaaaaactt gacagccagt atataacccc tgttcagctt
     2341 gtaaagcagg ctaaagaaca caaaacgcca gtcattgcct ttacatacaa tgaaccatca
     2401 attcagtatg agtacatcta cgacactgta cagatagcaa aaaaagacaa catatcctgt
     2461 gtcatcattt ctaatggtta tctcaataaa catgccagcg agatgttata tccgttgctc
     2521 aatgccatca agattgattt caaagcatat tcacagaaat tttattctga aatatgtggt
     2581 gggaatctat cgccagtact ttcaaatatt gaactggcta agaaaaaaaa tgtatggctt
     2641 gaactggtaa atctagttat tcccacatta aatgacaatc ctgatgatat cggggcaatg
     2701 gttcgtttta ttaaaactca ttgtggcaga acaacgcccc tgcacttcac acggttttat
     2761 ccaatataca aattaaaaaa tattccgcct accccagtat caactcttga acgctgcagg
     2821 gaaattgctc ttgctgaagg acttaaatat gtgtatatag gcaacgtccc agggcatagc
     2881 gccaacaata cctattgcca taactgcggc aaacctgtta tagaacgaag cggtttttat
     2941 acaacaaaaa acaaacataa atcagggaaa tgcccatact gcaatactgc aatccctggt
     3001 atatggacat tatgaaatac aaccgtgtat ttttatatat atctacgttc ttcattggca
     3061 tttcaggtag cattgcccag gcaattatga ttcgtgaatt actcatgttt ttcaggggca
     3121 atgaactatc tattggtatt attatggtat tgtggtttgc tggtatcacc gctggtgcag
     3181 ccataagcaa aaaaataaaa gcgcatcaat tcagcagtat tgcccataac attttacttc
     3241 atcccagctc agtatgtatt tcgcttatac tgctatactg tacacctata ttttatcaca
     3301 catatgattc attttatcca cttgaagtgg aacttattat tgcagtggtg tgtcttttac
     3361 cgcaatgctt tattattgga tatatctttc cagttttagt taatgcatgc tcacgtttaa
     3421 caattaacaa tcccggaact gtagtatttt tacttgaatc acttggcgca tttactggtg
     3481 gaatagcatt tacattttta atcatacaaa aaattaaccc tttgggaacc acactcattc
     3541 ttttcatcat tgcctgcatt atttcattca ctgttgtaca acaaagaatc aagcgtttgg
     3601 cgatagttat tgctatggtt attgttgcct ttgtaatagc tcactcatca tacgttgaga
     3661 agaaactgct ctcactgtca tttgctgcaa atcatcctgg tacactactt gaatatagac
     3721 gtacccccta tcagatgatg tttgttgcag gtgttgatga caccattgca ctgtatggaa
     3781 acaataacct gtatgcacaa ttaccagacg actaccaggt gcgaccgata attcatgcat
     3841 tactttccac gggctctgtg ttaaaagata cagatatcct catttcagga gatactgcct
     3901 tcctgcatta cgtttttgca tatgcaggtg cttctattga ccatgttact gttgacccgg
     3961 tgatgtggaa tattgtacag caaaaaataa attcaatata tccagatttc cccacaaaat
     4021 taataaatgt ttactatgac gatacacgcc attacttaaa atcaacaggc aaaaaatata
     4081 atgctgtcat cattttgcca ccaccccctg acagcattat gcataaccgg ttatatacag
     4141 tagaattttt taaagactgt aaactacatt taacacataa tggtgtgtgt attatccaga
     4201 ttgaaggctt tgccaatatt atgaatcctt ttcttaggag atatgttgca tcagttataa
     4261 atagcttcag gcaagtattc ccttcatcac ttatttcagc aggggatact atttactgta
     4321 ttggatttaa ttacaaccgt gatactgata ccagtaacta tctgtcacaa tatttaaaga
     4381 atttttcagc atctcctatt tcaaaaacaa tacgcattct tgttccgggc tttagtcccc
     4441 aggaatttaa aatgtatttt caggaatcgc aacaggaata tcttgcaaaa caattacaat
     4501 ctgaacatat cataaacagt gaccttttac caaaaggata ctttgaatat ttagctaatt
     4561 cattaaaaca atccagttca gaagcagaac gtattattac caatcctttg ttgatgatac
     4621 ttttgttaat aataatagta attttaagtt tatggtattt caattcacgg ggaactcaac
     4681 acataatttt tggcagcata atatttgtaa gcggatttat cagcatggca gcaatgatca
     4741 ttgcacttat tgcgtatcaa aatgtgtttg gtgttttgta ttataaaatt gctctttgta
     4801 atgcattgtt tatgttagga ttagcaatag gcagtgcatc cacatttaca caaaaagatt
     4861 tttttattac acggattatg gtattaatta ctggcttagt gatgttattt tgttttacta
     4921 tctacaataa cgcaatacta ttgtatgttt caattatact cattgcagca accaccggtt
     4981 cttttatacc actattactt aacaggaaaa ccaatgatat acagctttta gcttcaactc
     5041 ttgatgctgc cgatcacagt ggtgcattat gtggtgcatt gataacacca tttgtattgc
     5101 ttccgttttt tgggatttat tcgctattga tattgattgt aatggggttt attctgattg
     5161 tttttagatt acaaaattat tctcatatct gactgtctct ataacttcat cagctttttg
     5221 tatttgctct tctgtacctg taacagctaa ccatacacaa ccttcagcac cacttactcc
     5281 ccctgct
//
LOCUS       DAOSZP010000080         5219 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_13274_length_5219_cov_3.165569, whole genome shotgun sequence.
ACCESSION   DAOSZP010000080 DAOSZP010000000
VERSION     DAOSZP010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5219
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13274_length_5219_cov_3.165569"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            210..866
                     /locus_tag="PK348_02695"
     CDS             210..866
                     /locus_tag="PK348_02695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65154.1"
                     /translation="MKHWMLNFYCKETTSSLSTIHDDMTGWTSTPLGLFSIEGIFSGY
                     DGEVYVFDTYSAYSFYRFNGTDAELMQSFQSTPPTSGTYLGGFRTKNYFYIWYDGGND
                     SIFRTNGSVDINYNDTTAWSNVVDVAVTDDDKMYAIIFVSGTYNLLHIKGPMDFTTVL
                     FLGTTGTFKIDCLDSKHIIVAAYGNTDGYNGLFIYNVESKKIEKFITAEDAIALYVHR
                     "
     gene            1109..1624
                     /locus_tag="PK348_02700"
     CDS             1109..1624
                     /locus_tag="PK348_02700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65155.1"
                     /translation="MIQLRSFVILSGLLISLPFISCSTIQLAQQKTQLYSTTSDTLTL
                     PKSKERIDKTPTYELFFELLDNTRCLDISVIAQDRNGKRFRCAFILEALVDTSLYPVK
                     PLKPIYTEFARNFDINWRFQSNIFICSDENDPLKRLTKGNYRLKISAFSNNYYTFTID
                     IKSSVPVEFRK"
     gene            1656..2138
                     /locus_tag="PK348_02705"
     CDS             1656..2138
                     /locus_tag="PK348_02705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65156.1"
                     /translation="MPKHYDDIESEDLYEMALQWASQGFYDKAIELVKQSIELNPNFI
                     GAYIDLAHYYAKKHRYNDAFHVLKKASKIDMCFHRLNYLMAKYAYKNGDYIAASKYID
                     RAIELSAEKLYIKAKAVIGKKSVKKLTKKKTVIIHKIISYCKIMNYIFYVFSNIYIFL
                     "
     gene            2250..2771
                     /locus_tag="PK348_02710"
     CDS             2250..2771
                     /locus_tag="PK348_02710"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HXK65157.1"
                     /translation="MKKILLVLLVFFTASSVFAAGIGIKGGYSKMYDDYADAFDDTWN
                     IGIVFDLGTFLFNNLQFRPGIDYLSLENEDANFEYADVYGIHLDWYWFFMGKKQFSPF
                     LGFGPALNYYSFDDDRTNNDDSDAGIEGFAGVDFDLSGPWKLMLELRYLWHDIADRDT
                     TIVKLNVGLLYYF"
     gene            complement(2814..3374)
                     /gene="pyrE"
                     /locus_tag="PK348_02715"
     CDS             complement(2814..3374)
                     /gene="pyrE"
                     /locus_tag="PK348_02715"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007222521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HXK65158.1"
                     /translation="MNDNQRLFEILYQKSFMYRDNPPFTLVSGKQSPYYFDCKATTLD
                     PEGCILVGRCMFELIKDLPADAIGGLTLGADPISLSTAMHAFAQGKVLYPLIVRKEPK
                     KHGTQKWIEGNTANVKNVIAIDDVITTGGSTITAIERLTESGLSVIAAAVIIDREEGG
                     REAIEEKGITVLSLFKRSDFDKKRLE"
     gene            complement(3371..4672)
                     /locus_tag="PK348_02720"
     CDS             complement(3371..4672)
                     /locus_tag="PK348_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018052413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HXK65159.1"
                     /translation="MNLFDKNTYIPLAERMRPKTIEEFVGQKHLLSKGKLLYSIFEQK
                     KALSLILWGDPGTGKTTLAHLIASACNMDSYFISAISAGVADVRKVIDKGKANRTAGV
                     QTLLFIDEIHRFNKAQQDAVLGAVENGDIVLIGATTENPSFSIIAPLLSRTRVLRLYP
                     LQEDDLRAIVTAAIANDQELSHKNITLDEAALDALVQLAMGDARRALNILEAAAFLKG
                     QGTIDKDTVFEAYKEQSIAYDRAGDKHYDTISAFIKSLRGSDPTAAVYYLARMLVAGE
                     DPEFIARRMVIFASEDVGNAYPLALTVATSGMLAVKNVGLPECEIILAHMATFLASCP
                     KSNASYMALVNAKEIASSDVHEIPLYLRNAPTQLMKQLGYAKGYKYPHDYPGHFVDEE
                     YLPKEIKDLIFYAPSEEGNEKNIKERLTRLWPKKYKKEKGE"
     gene            complement(4683..>5219)
                     /locus_tag="PK348_02725"
     CDS             complement(4683..>5219)
                     /locus_tag="PK348_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019597085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HXK65160.1"
                     /translation="RRALAIQMIINHEYGLAKNQNPLQGSFIIEELTNLVEEAVIAEF
                     DNISARGGVLGAMETMYQRNKIQEESLYYENKKMSGELPIIGVNTFLSKNGSPIQIPD
                     EVVRATPEEKDTQLASLEAFHAVWKQESTVALEKLKTAVKQHENVFETLMDVAKYCSL
                     GQLTGALYSVGGMYRRSM"
BASE COUNT         1594 a          982 c          936 g         1707 t
ORIGIN      
        1 cggatgccaa tatccttcgt caggtacgtc aaaaactctt ccattcgctt tgcatttgcc
       61 tgcatagtca tatcaattcc tgtgaagttt aattgataag aaatcgcttg tcaggcggca
      121 tgggtgtccg cgggtagttg aaaccagtct gaagaagcgc ttcatggtgt gcaaggtaac
      181 aggcgcagcc tgagacgtca aacacagatt tgaaacactg gatgctgaat ttttattgca
      241 aagaaactac atcttcatta tccaccatac atgatgacat gactggttgg acaagtactc
      301 ctctgggttt attttctata gaaggtatat tcagtggcta tgatggagaa gtatacgtat
      361 ttgatactta ctctgcttat tctttttata gatttaatgg tactgatgct gaactgatgc
      421 aatcttttca aagtactcct ccaacgtctg gaacatatct gggtggtttt agaactaaaa
      481 attattttta tatatggtat gacggtggaa atgattctat atttagaact aatggtagtg
      541 tagatataaa ttataatgac actactgcat ggagcaatgt cgttgatgtt gcagttactg
      601 atgatgataa aatgtatgct ataatattcg ttagcggaac atataatctg ttacatataa
      661 aagggccaat ggactttacc acggtattgt tcctgggaac tacaggaaca tttaagatag
      721 attgtcttga cagcaaacac attatagttg ctgcttatgg aaatactgat ggttacaatg
      781 gactttttat ctataatgtt gaaagcaaaa agattgaaaa attcataact gctgaggatg
      841 ctattgcact ttatgttcac agatgaaata aacaatagtt ataattattt ttttacatga
      901 aacgttttga tattatacta ctcaattttt taatttaaaa aaaatggaca taatatacca
      961 tggtgtatag tagtattatc taacgataaa ctttcaatga aaggtaaaat ggtttaaaac
     1021 ataatgcatg tatttttaat acagtacagg tattctgtgg tacaaccaag agtgtatgat
     1081 aatttcaaag agattattac tggtatttat gatacaattg cgtagttttg ttatattatc
     1141 tggcttgctt atatctctac catttatttc atgctcaacc atacagttag ctcaacaaaa
     1201 aacacagcta tattccacta cgtcagatac actcacattg cccaaaagca aagaacgcat
     1261 tgataaaact ccaacgtacg aacttttttt tgagcttctt gataacaccc gttgtcttga
     1321 tatttctgtt attgcacagg acagaaacgg caaacgattt cgctgtgcat ttatactgga
     1381 agcattagtt gatacatcgt tatatcctgt aaaaccactt aagccaatat atactgaatt
     1441 tgccagaaat tttgatataa actggcgctt ccaaagcaat attttcatat gcagtgatga
     1501 aaatgatcca ttgaaaagac ttactaaagg gaactatcgc ctgaaaatat cagcattctc
     1561 aaataattat tatacattca ctatcgatat aaaatcatcg gtgcctgtag aattcagaaa
     1621 ataaaatttg ttatgttaac tcagataggt actatatgcc aaagcattat gatgacattg
     1681 aatctgaaga cctgtatgaa atggcattac aatgggcttc acagggattt tacgataaag
     1741 ctattgagct tgtaaagcaa tcaatagaac tcaatccaaa ttttattggt gcatatattg
     1801 atttagcgca ttattacgca aaaaaacaca ggtataacga tgcattccat gtattaaaaa
     1861 aagcctcaaa gattgatatg tgctttcacc gtttaaatta ccttatggca aaatatgcat
     1921 acaaaaatgg cgattatatt gcagcatcaa aatatattga tcgggcaata gaactttcgg
     1981 ctgaaaaatt gtatattaaa gcaaaagccg ttattggtaa aaaaagtgtt aaaaaactta
     2041 caaaaaaaaa aacagtaata attcataaaa ttatttctta ttgtaaaata atgaattata
     2101 tattctatgt attcagcaat atatatattt tcttgtagaa ctaaattcat gttttaaaat
     2161 gtttatttta taacacaaat tttacttgca cttttaacta tataaccttt tactatatat
     2221 attgtaaaat aactatttca ggaggttata tgaaaaagat attacttgtg ttactggtat
     2281 tttttacagc ttccagtgtt tttgctgctg gcataggcat caaaggtgga tactcaaaga
     2341 tgtatgatga ctatgcagat gcatttgatg acacatggaa tattggtatt gtttttgatc
     2401 ttggtacatt tttatttaat aatctgcagt tcaggccggg tattgattac ctgtcactgg
     2461 aaaatgaaga tgccaatttt gaatatgctg atgtgtatgg cattcatcta gactggtact
     2521 ggttctttat gggcaaaaaa caattttcac cattcttagg gtttggtcca gcgttgaact
     2581 actacagttt tgatgatgac agaaccaata atgatgattc agacgcaggt attgaaggat
     2641 ttgcaggtgt tgattttgat ttaagtggtc catggaaact tatgcttgaa ttgcggtatt
     2701 tatggcatga cattgctgac agagacacaa ctattgtcaa actaaatgtt ggtttgttat
     2761 actattttta attattaaaa aaaagaggcg gttatctacc gcctctttta attttactcc
     2821 aaccgttttt tatcaaaatc agaacgttta aataacgata gtactgtaat ccctttttct
     2881 tctattgctt cacgtccacc ttcttcgcgg tcaataataa ctgctgcggc aatgacgctg
     2941 agccctgatt cagtcaaccg ttcaattgca gtgatggtgg aaccaccggt ggtgattaca
     3001 tcatcaatgg caatgacgtt ttttacatta gccgtattgc cttctatcca cttttgagtg
     3061 ccatgtttct ttggttcttt cctgacaatt agtggataca aaaccttgcc ctgagcaaac
     3121 gcatgcatag ccgttgacaa tgaaatggga tcggcaccta atgttaaccc accaattgca
     3181 tcagctggta aatcctttat taattcaaac atgcacctgc ctactaaaat acaaccttca
     3241 ggatcaagtg ttgttgcctt gcagtcaaag taataaggac tttgtttgcc actaaccagt
     3301 gtaaatggcg gattgtccct gtacataaat gacttctggt ataaaatttc aaataatctt
     3361 tggttatcgt tcattcacct ttctccttct tgtacttttt tggccacagt ctggtaagcc
     3421 gttcttttat attcttttca ttcccttctt cagaaggtgc gtaaaaaatc aaatctttta
     3481 tttctttggg aagatattct tcatcaacaa aatgtcctgg ataatcgtga ggatatttat
     3541 agccttttgc atatcccagc tgcttcatga gttgtgtggg agcattacgc aggtagagcg
     3601 gaatttcatg tacatcactt gacgcaattt ctttggcatt aaccagtgcc atgtaactgg
     3661 cattactttt aggacatgat gctaaaaatg ttgccatgtg ggcaagtata atttcacact
     3721 caggcaagcc aacatttttc acagccagca tcccacttgt tgcaacagta agcgccagtg
     3781 gatacgcatt ccctacatcc tcagaagcaa atattaccat acgccgtgca ataaactcag
     3841 ggtcttcccc tgcaacaagc atgcgtgcca ggtaatacac cgcagcggtg gggtcagagc
     3901 cacgtaatga ttttataaac gccgaaatag tatcgtaatg cttatcgcca gccctgtcat
     3961 atgcaatgct ctgttccttg taggcttcaa aaactgtatc cttatcaatt gtgccctgcc
     4021 ctttcagaaa tgccgcagcc tcaagtatat tcagtgcacg tcgggcatca cccattgcaa
     4081 gctgtaccag tgcatccagt gcagcttcat caagcgttat atttttgtgc gatagttcct
     4141 ggtcattggc tattgctgca gtaactatcg cccgcaaatc atcctcctgc aatggataca
     4201 gccgcaatac gcgggttcgt gacaataatg gcgctatgat gctgaaagac ggattttccg
     4261 ttgttgcccc aatcagaaca atgtcaccat tttctactgc accaagaacg gcatcctgtt
     4321 gagccttgtt aaagcgatga atttcatcaa taaaaaggag tgtttgaaca cctgcagttc
     4381 tattggcctt ccccttatca ataaccttac gtacgtcagc taccccggcc gagatagcgc
     4441 ttataaaata tgaatccata ttacaggcac tggcaatgag atgagccaga gtggttttgc
     4501 cggttccagg atcgccccac aggataagcg acagagcttt cttttgttca aagatagaat
     4561 acagtaactt cccttttgaa agcaaatgct tttgccccac aaactcttca atagttttgg
     4621 ggcgcatccg ctctgctaag ggaatgtatg tgtttttatc aaataagttc attatccata
     4681 acttacatac tgcgcctgta cataccacca acgctataca gggcaccggt taactggcct
     4741 aatgaacaat attttgcaac atccattaat gtttcaaaca cattttcatg ttgtttcaca
     4801 gcagttttta atttttctaa agctactgtt gattcctgtt tccatacagc atgaaatgct
     4861 tccagtgaag caagctgagt atctttttct tcaggagttg cccgtaccac ttcatcagga
     4921 atttgaatag gacttccgtt tttagaaagg aatgtattaa cgccaatgat aggcaactct
     4981 cctgacattt ttttattttc atagtacagg gattcttcct gtattttgtt acgctgatac
     5041 attgtttcca tagcacccag tacgccacca cgagcactta tattgtcaaa ttcagcaata
     5101 acagcttctt caactaaatt ggtcaattct tcaatgataa aagaaccctg cagtggattt
     5161 tgattttttg caagtccata ttcatgatta ataatcatct gtattgctag tgctcttcg
//
LOCUS       DAOSZP010000081         5203 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_13326_length_5203_cov_3.175214, whole genome shotgun sequence.
ACCESSION   DAOSZP010000081 DAOSZP010000000
VERSION     DAOSZP010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5203
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13326_length_5203_cov_3.175214"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..403
                     /locus_tag="PK348_02730"
     CDS             <1..403
                     /locus_tag="PK348_02730"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HXK65161.1"
                     /translation="LLRFKNGKVEYVNAAHTEMLLKRKESNNVIVCTHKERDIKGMFL
                     GKSVFQTTYTPLQFSMWPGDQLLVYSDGLTEQKNKDAEEFGFERLQEAFLHAQGQTAQ
                     EICDYIIKTFNEFRGDVGLGDDVTVIVLQKK"
     gene            complement(404..1594)
                     /locus_tag="PK348_02735"
     CDS             complement(404..1594)
                     /locus_tag="PK348_02735"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HXK65162.1"
                     /translation="MAHGEKINKRKENKRKHIVDIFRQHPVLSKAEAKRYSGYSMDTI
                     MTSFKILKNTGIIVPTEGKQKEKGRKALFYTLNHEKQVYLGITFNQKAIYTSIVSFSN
                     KVLAKTKVGISSSISKEEFTRVMLQQIDEVLSKNPVFTNNLVAVGCTVPGDIDTRNGI
                     LRSYTLMPQLKDFNFNELLSTKFPNTRIFFDHNISSMISYILSTSDINQRYDRIMVVS
                     ARAASAVGLIYKGAIVNSRGEIGHIKVCDDNHQCICGRSGCLDCYFSYEAFVKLLQQQ
                     NAIDENECYDNVIAKLNNHYTNRTEGIFEKLDTALKFFSEALLDVTNVFSPDYILLTG
                     DLFEIYNDPVAQIDIHIKDLFHDTGYITMYKRALMEFQSIKTESIAHGVCLSLIKQDW
                     GYSA"
     gene            1745..3184
                     /locus_tag="PK348_02740"
     CDS             1745..3184
                     /locus_tag="PK348_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015920004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXK65163.1"
                     /translation="MNQERKITFKNLLSYGVGDIFGGGSFVVINLLFIYFLTDIAKLN
                     PALAGLVVLAGKAWDAISDPIMGYISDTTKSKYGRRRIFFLLGIFPIILSFIAMWLPF
                     SSESQIIKFIYYACAYVFFSTAFTMVMIPYSALNAEMTREYKDRTRLSGARLIFSQIS
                     SLISGTVPKMVVDAFTDKGQGFLVMSIVFAVFFALPWIIVFFGTWELPDVKVENKSLT
                     DFLKNLLVIWTNKSFRVHIGLYICAYSALDLIMAMFIYYLTYYIQKPHIFTMCLGAML
                     VTQVLFLPVYVFIANKKGKGFSYRVGVSIWAIALVSTLLFDSATPQQYIVALSVLMGA
                     GMSAGTMIPWAILPSVTDVGELITGKQQAGVYAGAMTFIRKLVQAVVLAIFGLVLQTI
                     GYKANTVQSPETLEYLRLMFALVPSTLMLCGILISLKFKITPVTHPILIQEINRLKSG
                     GSKDAVEPFVKKVCEVLTGYKYENLFKKQ"
     gene            3194..4456
                     /locus_tag="PK348_02745"
     CDS             3194..4456
                     /locus_tag="PK348_02745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="fucose isomerase"
                     /protein_id="HXK65164.1"
                     /translation="MKEVTVGVVCFARKTFDYSAAKEIYGSIQSQLKTIKAVQWHCYN
                     ELVFEVEDAQKAIAFLKKNNIDALVCISGTFHLGHLALELIKHVSVPVMLWGLPELPY
                     DGGKIRLNSICGVNLDASNLYKAGIRNYYAHFGNTIDTDWIGAIRIISMLKNAKIGLV
                     GYHAHGFFNLDTWHPALYAQCGTMIEHYELPHVFNYPVNKQYQNQYADDIESNFDTGD
                     INSTQKQKVAELSSRLHSFMEDNNLTALAIRCWPEFAAEFGIAPCAAMSLVQAHNFVI
                     ACEGDVDGALSMLAHKALGAQTPFLFDFSQVNLEENFALLWHCGVAPFTLKDTASTAS
                     LDTYFAAGKGVTAGFVLKPGDVSICRFDFDGSNYRLLLLKAHGVTMDKLLKGTYLKVT
                     FDVPVEIFLIPLFIMVLLITHQWCTGIS"
     gene            4566..>5203
                     /locus_tag="PK348_02750"
     CDS             4566..>5203
                     /locus_tag="PK348_02750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HXK65165.1"
                     /translation="MHSVKKPCIAVGFGNAVYSMKEGNFKLKDSITSKQYLKHFTVKE
                     DKNNITIQLQDAIELTITADTYCTINIKAKKPFNRFWLHCVADIHEHIYGCGEQFSKL
                     DLKKKKLPLWVQEQGVGRGHDLITLLANVLYGAGGTWYTTYYPQTTFVSSSNYFIHCN
                     ASSYAEFEFKKKEHILHFWQIPESVVVGVYEKPAECIKAVGDLLGRQPRLPQ"
BASE COUNT         1594 a          853 c         1026 g         1730 t
ORIGIN      
        1 acttttacgt tttaaaaatg gtaaggttga atatgtaaat gctgcccata cggagatgct
       61 tttaaaacgt aaagaaagca ataatgttat tgtatgtact cataaagaaa gagatattaa
      121 ggggatgttt ttagggaaaa gtgtattcca aactacctat acaccccttc aatttagcat
      181 gtggcctgga gatcagttgt tggtatacag tgatgggctt actgaacaaa aaaataaaga
      241 tgctgaagaa tttggatttg agaggcttca agaagctttt ttgcatgctc agggacaaac
      301 tgcacaggaa atttgtgatt atatcattaa aacgtttaat gaattcaggg gtgatgtagg
      361 attaggtgat gatgtaacag ttatagtatt acaaaagaag taattatgct gaatatcccc
      421 aatcctgctt tattaaactc aagcataccc catgagctat tgattcagtt tttatactct
      481 ggaattccat taatgcccgt ttgtacatgg tgatatatcc ggtatcatga aatagatctt
      541 tgatatgaat atctatctgg gcaacaggat cattgtatat ttcaaacaaa tctccagtaa
      601 gaagaatata atcaggtgag aaaacattag ttacatcaag taatgcttca gaaaaaaact
      661 tcagtgctgt atcaagcttt tcaaatatac cttctgttct attagtataa tggttgttaa
      721 gttttgctat tacattatcg tagcattcgt tttcatctat ggcgttttgc tgctgtaata
      781 atttgacaaa tgcttcatag gaaaaatagc aatccaggca tccacttctt ccacagatac
      841 attggtggtt atcatcacag actttgatat ggccaatttc ccctctactg tttactatcg
      901 cccctttata aattagtcca acagcacttg ctgcacgtgc tgaaacaacc attatcctgt
      961 cgtaacgctg attaatatca gaagttgata agatgtatga aatcatgctg ctgatattat
     1021 gatcaaaaaa tattcgtgta ttgggaaatt tagtggataa cagttcatta aaattaaaat
     1081 ccttcagttg aggcatgagt gtatatgatc gtaagattcc atttctggta tcaatatcgc
     1141 caggtactgt gcaacctaca gcaacaagat tgtttgtaaa cacaggattt tttgataaca
     1201 cttcgtcaat ttgttgtagc ataacccggg taaattcttc ttttgaaata ctgctggaaa
     1261 tcccaacttt tgttttggct aaaactttgt tgctgaaact aacgatagat gtgtatattg
     1321 ctttttgatt aaaggtgata cctaaatata cctgcttttc atggtttaga gtatagaata
     1381 atgctttacg gcctttttct ttttgtttac cttcagtagg tactattata cctgtatttt
     1441 ttaaaatttt aaaagaggtc atgatggtat ccatagaata cccggaatat cgttttgctt
     1501 cggcttttga taatacagga tgttgccgga agatatcgac tatatgctta cgtttatttt
     1561 cttttcgttt gttgattttt tcaccatgtg ccataacatt cttcctgagt atgataattt
     1621 tcttgacata ttatcgataa cgattttatt attaaactat caaaatgata gttgatatta
     1681 taacattatt gctcattgtt tttacttatg caacaatttt ttataattta aaacaggtgc
     1741 gcttatgaat caagaaagaa aaataacttt taaaaattta ttatcgtatg gcgttggtga
     1801 tatttttggt ggtggttctt ttgttgttat aaatctttta tttatatatt ttttaactga
     1861 tatagcaaaa ctcaatcctg cactggctgg acttgtagtg ctggctggta aggcatggga
     1921 tgcaatcagt gacccaatca tgggatatat ttctgatact actaaatcaa aatacggtag
     1981 acgcagaata ttctttttac tggggatttt cccaatcata cttagtttta ttgccatgtg
     2041 gctgcctttt tcatctgaaa gccagataat aaaatttatt tattacgcct gtgcgtatgt
     2101 attttttagt acagccttta ccatggtgat gattccgtat tcagcactca atgctgagat
     2161 gacacgagaa tataaagata ggacgcgcct ctcaggtgca cggttaattt tctctcaaat
     2221 atcatcactc atatcaggta ccgtacctaa aatggttgtt gatgcattta cggataaggg
     2281 gcagggtttc cttgtaatga gtattgtttt tgcagttttt tttgcgttgc catggattat
     2341 cgtatttttt ggaacatggg aactgcctga tgtaaaggtg gaaaataaat ctcttactga
     2401 ttttctcaaa aatctcttgg ttatatggac aaacaaatcg ttcagggttc atattggttt
     2461 gtatatctgc gcatattcag cacttgattt aatcatggca atgtttattt attatcttac
     2521 ctactatatt caaaagccgc atattttcac catgtgtctt ggtgccatgc ttgtgacaca
     2581 ggttttgttt ttgcccgtat atgtatttat agcaaataaa aaaggtaagg gtttttccta
     2641 tcgtgtaggg gtaagtatat gggcgatagc tctggtaagt actcttttgt ttgatagtgc
     2701 tactccgcaa caatacatcg ttgccctttc agtactgatg ggggctggca tgtcggcagg
     2761 aaccatgata ccctgggcaa ttttgccttc agtgactgat gttggggaac tcataaccgg
     2821 caaacagcag gcaggtgtat atgcaggtgc tatgacattt atacgaaaat tggtacaggc
     2881 agttgtgctg gctatatttg gtttagtgtt gcaaactatt ggatataaag caaataccgt
     2941 tcaatcccct gaaacattag agtatctacg cttgatgttt gcactggttc catcaacgct
     3001 tatgctctgt ggtattctca tttcattaaa atttaaaatt acaccggtaa cacatccaat
     3061 tcttattcag gaaattaaca gattaaaatc cggtggaagc aaagatgcag ttgaaccatt
     3121 tgttaaaaaa gtttgtgaag tattaaccgg ttataaatat gaaaatcttt ttaaaaagca
     3181 ataggtgata ataatgaaag aagtaactgt tggtgttgta tgttttgctc gtaaaacctt
     3241 tgattacagt gcagctaaag aaatatatgg ttccatacaa tcacagttaa aaacgattaa
     3301 agctgtacag tggcattgct acaatgaact tgtatttgaa gtagaagatg cacaaaaggc
     3361 tattgccttc ttaaaaaaga acaatataga tgctcttgtg tgtatcagtg gaacatttca
     3421 tttagggcac ttagctctgg aattgataaa acatgtcagt gttcctgtga tgctgtgggg
     3481 attgcctgaa ttgccatatg atggcggtaa aatacggctc aattcaattt gtggcgtaaa
     3541 tttagatgca agcaacctgt ataaagcagg gatacgcaac tactatgctc attttggtaa
     3601 caccatagat actgactgga ttggtgctat acggattatt tccatgctta aaaatgccaa
     3661 aattggactg gttggctatc acgcccatgg attttttaac ctggatacct ggcatcctgc
     3721 attgtatgca cagtgtggca caatgattga acattatgag ttaccgcatg tattcaatta
     3781 tcctgttaat aagcagtacc aaaaccaata tgctgacgat atagaaagta attttgatac
     3841 aggtgatatc aatagcacac agaaacaaaa ggttgcagaa ttaagttcca ggctacacag
     3901 ttttatggaa gataataacc ttactgctct ggcaattcgg tgctggcctg aatttgccgc
     3961 tgaatttggc attgcgccat gtgcagccat gtcgctggta caggcacata attttgtcat
     4021 tgcctgtgaa ggtgacgttg atggtgcact atccatgtta gctcacaaag cgctgggtgc
     4081 gcaaacacca ttcttatttg atttttcaca ggtaaatctt gaagaaaatt ttgcattgct
     4141 gtggcattgc ggtgttgcgc cattcacact caaagatact gcaagcacag catcgcttga
     4201 tacatatttt gctgctggca aaggtgttac ggcaggtttt gtattgaaac ctggtgatgt
     4261 gtcaatatgc agatttgatt ttgatggcag taactatcgc ttattattat tgaaagctca
     4321 tggcgttaca atggataaat tgctaaaagg gacatattta aaagttacct ttgatgtgcc
     4381 tgtcgaaata ttcttgatac cattatttat aatggtattg ctcatcacgc atcaatggtg
     4441 tacggggatt tcataaaacc atttgagata gcagctcgca ttcaaaactg gggaatagtg
     4501 acatgctaaa aattgaacac aatgattcat cgttttcact atattacaag aatcgatgta
     4561 tcttgatgca ctctgtaaaa aagccatgca ttgcggtggg ttttggtaac gcagtctata
     4621 gcatgaaaga aggcaatttt aaactaaaag attccattac gtcaaaacaa tatttaaaac
     4681 attttactgt taaagaagat aaaaacaata ttacaataca attacaagat gctatagagt
     4741 tgacaataac tgcagataca tattgcacta ttaacattaa agcaaaaaaa ccttttaata
     4801 gattctggtt gcattgtgtt gccgatattc atgaacatat ctatggttgt ggtgaacagt
     4861 tttcaaagtt ggatttaaag aaaaagaaac ttcccctatg ggtacaggag cagggagtag
     4921 ggcggggaca tgatctgata acattgttag ccaatgtact atatggcgct ggaggtacat
     4981 ggtacacaac gtattatccg caaacaacgt ttgtatcaag cagcaattac tttatccact
     5041 gcaatgcatc gtcatatgca gaatttgaat ttaaaaagaa agagcatata cttcatttct
     5101 ggcagattcc tgaatcagtg gtagttggtg tgtatgaaaa accagctgag tgtatcaaag
     5161 cagtagggga tttattgggg cgacagccac ggctacctca gtg
//
LOCUS       DAOSZP010000082         5179 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_13397_length_5179_cov_3.179938, whole genome shotgun sequence.
ACCESSION   DAOSZP010000082 DAOSZP010000000
VERSION     DAOSZP010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5179
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13397_length_5179_cov_3.179938"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1150)
                     /locus_tag="PK348_02755"
     CDS             complement(<1..1150)
                     /locus_tag="PK348_02755"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013257091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HXK65166.1"
                     /translation="MADNDVVIVSACRTPIGSFGQSLKDVKAYELAALVMKEALRRAN
                     VEGSALSDVIFGNCLMGSDEANVARTAALKAGIPVEVPATSVQRQCASGMTAVVFGSQ
                     QLMVGDSDIVLAGGVESMSNAPYVLWKARWGLRLTHSELTDAMWELLHSGSGLLGEPY
                     IMGQTAENLAEKYSISREEQDQVAYESHKKAAKAIDSGRFNDEIVPVPIPQKKGEPKM
                     FEKDEHVRRDVTLESLSALKPAFKKNGTVTAGNASGINDGAAALVLTTAKRASELGLK
                     PLARIAGNAFAGVEPHLMGYGPVPAVQKLLKKTGKKLDDIDLIELNEAFAAQYLACEK
                     GLGLDRSKVNVNGSGIALGHPVGCTGARIIVSLIYEMKKRNAKWGIATL"
     gene            complement(1368..1712)
                     /locus_tag="PK348_02760"
     CDS             complement(1368..1712)
                     /locus_tag="PK348_02760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HXK65167.1"
                     /translation="MLNVSVNQLADVVVIDLEGEFDIDVIRDVEKAWNDALEKKPRVI
                     GFNCKELKFIDSSALGTLVKFMNSATNRKISLALFNVAPSVITIFKTSRLDKFFSINS
                     KEEFENKFLPVK"
     gene            1895..2260
                     /locus_tag="PK348_02765"
     CDS             1895..2260
                     /locus_tag="PK348_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65168.1"
                     /translation="MRFALLLKVLALILRIASKRNEDFKRFIGTVSVKIAIKTKNNKG
                     RTFIFNNGSVSSTRSLKQYDAALVFSDAKTGFTVLKEGTQEASFYAAATGTLYVEGMA
                     FFIQWFNDAVTIAMKKNKK"
     gene            2273..3928
                     /locus_tag="PK348_02770"
     CDS             2273..3928
                     /locus_tag="PK348_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015949727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HXK65169.1"
                     /translation="MSINGGHLIGKYLAKQGIQFAYGISGGHIEALLDGLQQYGIRSI
                     DVRHEQAAVMMAHATAIYTGTPGVCFLTAGPGFTNGITGIANACLDNAPVVVLCGRHP
                     LRDDHKGALQEMNQVDVVKPLVKWCATCYDTKRIPEYLDMAFRHATGGRPGPVFLELP
                     PDILGKAVDEFDFTPSVDEFKAVPDSSSLYKAAEIINSAKKPLFIGGSGIGYSNCTLE
                     LQAFIEKTGIPFILLNNGRGVLPDNHPLSIWNTGNVGLMMTMSQADVIVAAGIRFNWL
                     FQSGAVIPPTTKVVRIDIEPTEINRNRVADVGLLGDTGAALKALLPLVEQRNHQQWLD
                     MLTQAGYAFIDSEIKLRQNPSDPIHPIRLVAKAQEVFGTDALYVVDGGDTTYFGLVGF
                     QSRHKAGVLSQSAGLLGCLGAGIPFAIAAKLIHPDKQVVVLSGDGSFGFNGFEFDTAV
                     RHNIPFVCIVNNDCAWGMIKHSQELSIGKERVTCAELGIRRYEKVVEGMGGHGEFVEK
                     DEAIAPALIRAKESGKPACVNVITDPTVTSPATPLFYQSLKFD"
     gene            4175..4576
                     /locus_tag="PK348_02775"
     CDS             4175..4576
                     /locus_tag="PK348_02775"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HXK65170.1"
                     /translation="MKRIFEIHELSATLTPVFTKHNIIFAYLFGSIVNNESTPTSDID
                     IAVYCKGVMDDSVFEKKLSLHLDICRTLKIDNVDIIVLNKTTNLMLIDEVIRNGILLY
                     DTDTSLREKYEYFMIHKAIDFKTQRLYTMGI"
     gene            4576..4998
                     /locus_tag="PK348_02780"
     CDS             4576..4998
                     /locus_tag="PK348_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011365802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HXK65171.1"
                     /translation="MERIIQKIGKIHEYLTIIYTIKDDCINKIYTDPIYKGALLHYLY
                     LLSDSCISLAELVIKYKKLRVPQTYHEAIDILGENNILNPDFAYSFAKIAGFRNYLAH
                     DYDQLDTAIICNEILKRTKDIEEYLVQIKNSLGIKYYK"
BASE COUNT         1582 a         1035 c         1004 g         1558 t
ORIGIN      
        1 acagggtggc aataccccat tttgcattac gctttttcat ctcatagatg agcgatacga
       61 tgatgcgagc accggtacat ccaactggat gacccagcgc tatccccgat ccattgacat
      121 taactttgct tctgtcaagg ccaagtcctt tttcacaggc cagatactgt gctgcgaatg
      181 cttcattgag ttctataagg tctatatcat ctaatttttt ccctgttttt ttgagtaatt
      241 tttgaaccgc aggaacaggt ccatatccca tcaggtgtgg ttcaacacct gcaaatgcat
      301 ttcctgcaat ccttgccagt ggttttaatc caagttcact ggcacgctta gcggtagtaa
      361 gcaccagtgc agcagcacca tcatttatcc ctgaagcatt accagcagta accgttccat
      421 tctttttaaa tgcaggctta agggctgaca agctttccag cgtaacatca cggcgaacat
      481 gttcgtcttt ttcaaacatt tttggttcgc ctttcttctg gggtatggga acaggaacta
      541 tctcatcatt aaacctcccg gaatcaatgg ctttagcagc ttttttatgg ctttcatagg
      601 ccacctgatc ctgttcttcc cgtgaaatag aatatttttc tgctaaattt tctgcagttt
      661 gccccataat atatggctca cctaacaggc cacttcccga atgaagcagt tcccacattg
      721 catcagtaag ctcagagtga gtaagccgca aaccccatcg cgctttccac agcacatacg
      781 gtgcattaga cattgactca acaccaccag caagtactat atcagaatca cctaccataa
      841 gctgctggga tccaaatacc actgcagtca ttcctgatgc acattgccgc tgtactgagg
      901 ttgctggcac ttctacagga atacccgctt tgagtgctgc agtacgtgca acatttgcct
      961 catcagatcc cataagacag ttaccaaaaa ttacatccga caaagcactt ccttcaacat
     1021 ttgcccgccg taacgcttct ttcattacca gtgcagctaa ttcatacgct ttaacatctt
     1081 ttaatgattg cccaaaactg ccaataggcg tcctgcacgc ggaaacaatg acaacatcat
     1141 tatcagccat tacctatcct ccatgtatat agtattaaaa taaaagatgc ccaacaacca
     1201 ctacaggatt taagtggtta tatcaaacgc agtataaata tatatcccca tccccaaatg
     1261 gggcaggggt atacagtatt gttactgtat tacaaatata tttctaaaat ggtagggtta
     1321 ttttgcaagc ctttaattat ctggtaatca ttttttggat ataattatca ctttaccggc
     1381 agaaatttat tttcaaactc ttcttttgaa ttaattgaaa aaaacttatc caggcgtgaa
     1441 gtcttgaata tagtaataac tgatggagca acattaaaaa gtgctaaact aatctttcta
     1501 tttgttgcac tgttcataaa ttttaccagg gtgcccaatg ccgatgaatc aataaacttc
     1561 agttctttac agttgaaccc aataactctt ggtttttttt ctaacgcatc attccacgcc
     1621 ttttcaacat cacgaatgac atcaatatca aactcacctt caaggtctat gactactaca
     1681 tctgctaact ggtttaccga aacattaagc atggccaatc acctcttgta agctttatat
     1741 gctttttagt aacgtgtgaa ttgtacatta taaaaatctc catcgcaagc atatttttaa
     1801 atgcatcaga aaaaaataat ttacaaatac tacaagctat cgtacaaata ctgcagttta
     1861 tctcatgttt atatcttata taaagggtta tattatgagg ttcgcactac tgttaaaagt
     1921 actggcatta atattacgca ttgcttcaaa gcgcaatgaa gacttcaaac gctttattgg
     1981 cactgtcagt gtcaaaatcg caattaaaac aaaaaataat aaaggacgaa cattcatttt
     2041 taacaatggt tctgtctctt caacacgttc gctcaaacag tatgatgctg ctcttgtctt
     2101 ttccgatgct aaaaccggtt ttaccgtatt aaaagaaggc actcaggaag catcgtttta
     2161 cgcggcagca acaggaaccc tgtatgttga agggatggct tttttcattc aatggttcaa
     2221 tgacgcagta actatcgcca tgaaaaaaaa caaaaaataa acaggaggaa ttatgtcaat
     2281 aaacggtgga cacttaattg gcaaatattt agctaaacaa ggtatacagt ttgcctatgg
     2341 catatcgggc gggcatattg aggcattgct ggatggacta cagcaatatg gcatacgatc
     2401 aattgatgtg cggcatgaac aggctgctgt gatgatggct catgccactg ctatctatac
     2461 tggtacacct ggtgtgtgct ttttaaccgc aggtcccggc tttaccaatg gcatcaccgg
     2521 tattgccaat gcatgtttag ataatgcacc ggttgttgtg ttatgcggtc gtcatccgct
     2581 tcgcgatgat cataaaggtg cattgcagga aatgaaccag gttgatgtgg taaagccatt
     2641 ggtgaaatgg tgtgccacct gctatgatac taagcgcatc ccggaatatc tggatatggc
     2701 attcagacat gccacgggtg gaaggccagg gccagtgttt ttagaactac ccccggatat
     2761 acttgggaaa gctgttgatg agtttgattt tacacccagc gttgatgaat ttaaagctgt
     2821 gcccgattca tccagcttat ataaagccgc tgaaattata aacagcgcca aaaagccact
     2881 ttttataggc gggtcaggta ttggttacag caactgtact ctggaactgc aggcttttat
     2941 tgaaaaaaca ggtattccgt ttatactgct gaacaatggt cgcggtgttt tgcccgacaa
     3001 ccatccgctg tcaatctgga atactggcaa tgttggcctc atgatgacaa tgtcacaggc
     3061 tgatgtcatt gttgcagcag gcataaggtt taactggctt tttcaatcag gtgctgtcat
     3121 tccgccaacc acaaaagtgg tgaggattga cattgaaccc actgaaatca atcgcaaccg
     3181 tgttgctgat gtgggccttc tgggtgacac tggtgcagca ctgaaagcat tgttgccact
     3241 ggttgagcaa cgcaatcatc aacagtggct tgatatgctg acacaggcag ggtatgcgtt
     3301 tattgacagt gaaattaagc tgcgccagaa tcccagtgac cccatccacc ctatacggct
     3361 ggttgcaaag gctcaggaag tgtttggcac cgatgcgctg tacgttgttg atggcggtga
     3421 tactacttac tttggtctgg tgggttttca gtccaggcac aaagccggtg tactatcgca
     3481 atcggctggg cttctgggtt gtttgggagc aggcatccca tttgccattg cagccaagct
     3541 cattcatccg gataagcagg tagtggtgct gtccggcgac ggttcgtttg gatttaacgg
     3601 ttttgaattt gataccgctg tgcgccacaa tataccattt gtatgcattg taaacaatga
     3661 ctgtgcctgg ggaatgatca aacacagcca ggaactttct attggcaaag aacgggtaac
     3721 ctgtgctgag cttggcatcc gaagatatga aaaggttgtt gaaggcatgg gtggacacgg
     3781 tgaatttgtt gaaaaggatg aggcaatagc acctgcgctt atccgtgcaa aggaaagcgg
     3841 taaaccggca tgtgttaatg ttattactga tccaacggta acaagtccgg caacaccgct
     3901 attttatcag tcgttgaaat ttgattagaa gctttcttat ggccatttta agacgtgttc
     3961 cataatgagt tatcaattgg tgaaagttgc tacctgaatt gatgaacatt tgatgttatt
     4021 atcaatgtaa caaaatttta tatgatcgtc atttcaacga acatagtgag gataaatttt
     4081 aataaattaa aaataatttt tttcataaaa aaaattctta tttttacatg gtttttttca
     4141 atgatatgta tattaaattg aatagagaga tgatatgaaa cgtatatttg aaatacacga
     4201 attaagcgca accctaacac cagtatttac aaaacataat attatatttg catatttatt
     4261 tggttcaata gtgaataatg agtctacgcc cacaagcgat attgatattg cagtatattg
     4321 caaaggtgtt atggatgaca gtgtatttga aaaaaagcta tcattacatt tagatatatg
     4381 ccgaacacta aagattgata atgttgatat catagtatta aataaaacaa ccaatcttat
     4441 gcttattgat gaagttatcc gtaatggtat attgctctat gataccgata catcattgcg
     4501 tgaaaaatac gaatatttta tgattcataa agcaatcgat tttaaaactc aaaggctata
     4561 tactatgggt atctaatgga aaggatcata cagaaaattg ggaaaataca tgaatactta
     4621 acaatcattt atactattaa agatgattgt ataaacaaaa tatataccga tcctatttat
     4681 aaaggtgcat tgcttcatta tctgtatttg ttatccgact catgtataag ccttgcagaa
     4741 ctggtaataa agtacaaaaa attacgagtt cctcaaacat accatgaggc tattgatata
     4801 cttggggaaa ataacatact caatcccgac tttgcatatt cttttgctaa aatagcaggt
     4861 tttagaaatt atcttgctca cgattatgac cagcttgata cagccattat atgtaatgaa
     4921 atattaaaga gaacgaaaga tattgaagaa tatttggtgc aaattaagaa ttcattagga
     4981 ataaagtatt acaaatgata tatataaata ctatattaat cattacagta ctgttatgaa
     5041 agcactacag tttaatgtaa caataccgca atgggtagcg ctcaaagcgc tgggagcatg
     5101 tacaaaatct gtattttaca aaagtcccat tggcactctc aaactggttg aaattccaga
     5161 gccagaacct attaataat
//
LOCUS       DAOSZP010000083         5164 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_13432_length_5164_cov_3.660012, whole genome shotgun sequence.
ACCESSION   DAOSZP010000083 DAOSZP010000000
VERSION     DAOSZP010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5164
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13432_length_5164_cov_3.660012"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="PK348_02785"
     CDS             <1..234
                     /locus_tag="PK348_02785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65172.1"
                     /translation="DTMVDLTTIRELRELEASLRNEIQTAKGEIIRDLQKKEEYLTHL
                     IRVLIAKIDALEQEKKAVFQEKPKTNTRRLFRI"
     gene            complement(365..679)
                     /gene="cutA"
                     /locus_tag="PK348_02790"
     CDS             complement(365..679)
                     /gene="cutA"
                     /locus_tag="PK348_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008288422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HXK65173.1"
                     /translation="MEYCVIFCTVPTKEVGLEIANTLVSKQLAACVNIVPGLTSIYSW
                     KGEICNDQELLLIIKTRGNLYTNIEEAIKKIHPYEVAEIISLPITSGSYEYLQWIVNV
                     TK"
     gene            complement(707..1168)
                     /locus_tag="PK348_02795"
     CDS             complement(707..1168)
                     /locus_tag="PK348_02795"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013258215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HXK65174.1"
                     /translation="MNAKTVKESTITIVQQMTHQDANLAGNVHGGTIMKLIDNTGGIV
                     ASRHAGCLVVTASIDRLDFHSPVYVGDLLRLKASVNYVGTTSMEVGVRVEAENFITGE
                     VRHTASAYLTFVALDEHGKPKNVPPLQPETEDEKRRYQEAEIRRKKRLGNK"
     gene            1375..2028
                     /locus_tag="PK348_02800"
     CDS             1375..2028
                     /locus_tag="PK348_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006308437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HXK65175.1"
                     /translation="MPVNRSELVSFYSHMIGVVGAIIGLVMLIVTTNKPLIQILSVIY
                     GISVIMLFTASTLYHGLKQQENEDSLWRRLDHMAIFIMIAGTYTPVCYIYLDYKTALY
                     IIIAQWLLVVLGIIFKIYFFNAPRVIYTAIYLAMGWMAVLVIKDIVRIMDTVSLWYLF
                     LGGVAFTIGALFYIVKWPQKHHGYVGFHEIFHGFILAGAFFHYMMVYHSIQYIQNIH"
     gene            2175..2651
                     /locus_tag="PK348_02805"
     CDS             2175..2651
                     /locus_tag="PK348_02805"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HXK65176.1"
                     /translation="MVLTDYLNKKTILINPSVKDRWELIESMLDVAIKNKAVKSEYRE
                     TIKKTLFEREKSMSTGIGKGVAIPHCSCPYVDDVVMILALSKKGINFDSIDNLPVHIA
                     ILLIVPKDKISQHIKTLANIAKLMSDDALREALSNAKDAQDIIKILKEYEQKGKKQ"
     gene            2626..3669
                     /gene="ruvB"
                     /locus_tag="PK348_02810"
     CDS             2626..3669
                     /gene="ruvB"
                     /locus_tag="PK348_02810"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019414056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HXK65177.1"
                     /translation="MNKKEKNNNTPQSLISPLSLEEEDFEKKLRPQFLNEFIGQKQLT
                     ENLRIFIESAKLRKKPLDHVLLAGPPGLGKTTLAFIVANELGVNIKATSAPAIEKPGD
                     MASMLTNLEPMDVLFIDEIHRLSPTIEELLYGAMEDGELDIIVGQGVGAKSIKLQLAP
                     FTLIGATTRTGLLTSALRDRFGIPLRLDYYEIADLETIAKRTANIIGCIIDDMAAQEI
                     ARRSRRTPRIVNRLVKRVLDFAIVMKKGSIDLDIARYALDRLHIDSLGLDDMDRKILT
                     TIIDKYDGGPVGIKTIAISIGEEITTLEDFYEPYLVQIGMLKRTPRGRVAARLAYEHL
                     QLPYKGMDESSLF"
     gene            complement(3716..4315)
                     /gene="yedF"
                     /locus_tag="PK348_02815"
     CDS             complement(3716..4315)
                     /gene="yedF"
                     /locus_tag="PK348_02815"
                     /inference="COORDINATES: protein motif:HMM:TIGR03527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HXK65178.1"
                     /translation="MKEVDARGLECPKPVLLTKETIEKDNPDNLTVLVSNDTAVGNVT
                     RFAENCGYSVTTTQQGNYFILHLSKGTQAQSQPVSQPRTMESVNENIVFFITSNEFGQ
                     GHQELGQLLIKLFINTINEANMLPATMIFVNSGIFLTTEGSPVLPALQEIAQKGVQII
                     NCGTCLDFYKRKDQLKVGSISNMYTITETLMKATKVIRF"
     gene            4490..>5164
                     /locus_tag="PK348_02820"
     CDS             4490..>5164
                     /locus_tag="PK348_02820"
                     /inference="COORDINATES: protein motif:HMM:NF023863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3683 domain-containing protein"
                     /protein_id="HXK65179.1"
                     /translation="MDKIFKRDFKYREIPYNYTSFSDREIILKYFDAQTWDLVQALRA
                     KRRTGRSAKLLFENIGDIFIIDRNPYIQYDIVENPAKLKQLYKRHQRRLATVKEGANG
                     DQQVLEFIAKIEKLDEAFFRKLKETKKLQEKIFNRLKHITAAGNIHFSPFHRASHVTD
                     ATDWRVEYPVVVVYPDSVHEIQELVKAAKKLNLVIIARGGGTGLTGGAIPLLPNTMII
                     NTEKLNN"
BASE COUNT         1619 a          924 c         1010 g         1611 t
ORIGIN      
        1 gatacaatgg tagatttaac aacaatcagg gaattgcgcg agttagaagc atctttgcgt
       61 aatgagatac agacagcgaa aggtgaaatc atacgcgatt tacagaagaa ggaagaatac
      121 cttactcatc ttattcgtgt attaattgcc aaaattgatg cactggagca ggaaaagaag
      181 gcagtatttc aggaaaaacc aaaaacaaac accagaaggc tgttccgtat ataataatta
      241 gaatatataa ataaaatttt ttgaaaaaat cacggcctgt gatataaata gtatatgaca
      301 gcataggtga tgacctcacc atgcgtttct atataaaatt ccgtaccaca agtacggaat
      361 ttttttattt tgtaacattc actatccact gcaagtattc atatgaccca cttgtaattg
      421 gtaaagaaat aatttcagcc acttcatagg gatgtatctt ttttattgct tcttcaatat
      481 tagtatacag attacccctg gttttgatga taagaagtaa ttcctggtca ttgcatattt
      541 cgcctttcca tgaataaata gacgtaagac ctggaacaat attgacacag gcagctaatt
      601 gttttgaaac aagggtattg gctatttcca aacctacttc tttggtgggg acagtacaaa
      661 aaataacgca gtattccata cataaactcc tgttacaaaa tttactttat ttatttccca
      721 accgcttttt tcttctaatt tcggcttcct gatatcggcg tttttcatct tcggtttctg
      781 gctgtaacgg gggtacattt ttaggcttac catgttcatc cagggcaaca aaagtaaggt
      841 atgctgaagc agtatgtcgc acttctccag tgataaaatt ttcagcctca acgcgtacac
      901 caacttccat ggaggtagtt ccaacatagt taacacttgc ttttaaccgt aacagatccc
      961 caacataaac aggactgtga aaatccaatc tgtcaattga tgctgtcaca acaagacagc
     1021 ctgcatgccg ggaagcaaca atgcctccgg tgttgtcaat cagcttcatg atagtcccac
     1081 catggacatt tccagccaga ttggcatcct gatgtgtcat ctgctgtact attgttatag
     1141 tgctttcttt gacggttttt gcattcatac aattgatgta ctttcaatgg aataaaaaat
     1201 atatgaatta gtatattttt atatatgaaa aacataaatt tttgcaacca aaaaaacaaa
     1261 tcaattgtcc ataaatgaat gcattaaaaa atgtgagtat atggtactat cacttatggc
     1321 aataaagtat ttactgtttc tatctattta atcaatgaat ataaaaggta tagtatgcca
     1381 gttaacagat cagaacttgt atcattctat tcacatatga taggagttgt tggtgctatt
     1441 attggattgg taatgttaat tgtaactact aataagcccc ttatacagat actatcggtt
     1501 atatatggta tttcagtcat tatgctgttt actgccagca ccctgtatca tgggctgaag
     1561 caacaggaaa atgaagactc actatggaga agattggacc atatggctat cttcatcatg
     1621 attgcgggaa cgtatactcc tgtatgttac atctatttag attataaaac cgctctgtat
     1681 attattattg ctcagtggtt actggtggta ttgggtatta tcttcaagat atattttttc
     1741 aatgcgccgc gtgttatata tactgcaata tatttggcca tgggatggat ggctgtactg
     1801 gttatcaagg atattgtcag gattatggat actgtatccc tgtggtatct ttttctgggt
     1861 ggtgttgcgt ttaccattgg cgcactgttc tatatcgtaa agtggccgca aaagcatcat
     1921 ggatatgttg gttttcatga aatttttcat ggttttattc tggcaggagc ttttttccat
     1981 tatatgatgg tataccatag tatacagtac atacaaaata ttcattaaaa caaaaattat
     2041 tccctcactt tttaaagatc ccatatacgt tgtaactatc aaaaaaatta tattacactt
     2101 gacgaaattc ttatacgggt agtattttag ttgtaattat agaggttgat ttatttttgg
     2161 ggttgattgt atatatggtt ttaactgatt atttaaataa aaagacaata cttattaatc
     2221 cgtcggtaaa agatcggtgg gaattgattg aatcgatgct ggatgtggca attaaaaata
     2281 aagctgtaaa atccgaatat cgtgaaacca taaaaaaaac gttatttgaa cgtgaaaaat
     2341 caatgagtac cggtataggg aagggagtgg ccatacctca ttgcagttgc ccgtatgttg
     2401 atgatgtggt gatgatactg gcattgagta aaaaaggtat aaactttgat tcaattgata
     2461 atttgccagt gcatatagct attcttttaa ttgtccccaa agacaaaatc tcccagcata
     2521 tcaagacact ggcaaatata gcaaaactta tgagtgatga tgccttgcgt gaagccctat
     2581 ccaatgcaaa ggatgctcag gatattataa agattttaaa agagtatgaa caaaaaggaa
     2641 aaaaacaata acacacctca aagccttatt tctccgctat ctttagaaga agaggatttt
     2701 gaaaaaaaat tacgccctca attcctcaat gaattcatag ggcaaaagca acttactgaa
     2761 aatctgcgta tatttattga gtcagcaaaa ctgcgcaaaa aacccctgga tcatgtactg
     2821 cttgctggac cgccagggct tgggaaaacc acacttgctt tcattgttgc aaatgagctt
     2881 ggtgtcaata tcaaggcaac ttcagcacca gcaattgaaa aacctggtga tatggcttca
     2941 atgttaacca atctggagcc aatggatgtc ctgtttattg atgagatcca caggctttca
     3001 cccaccattg aggaattatt atatggagcc atggaagatg gtgagcttga tatcattgtg
     3061 ggccagggtg ttggtgcaaa aagtattaag ctgcaacttg caccctttac cctaataggc
     3121 gcaactactc gtacaggttt attgacgtca gcactccgtg accgatttgg catccctctc
     3181 aggctggatt attatgagat tgcggattta gagacgatag ctaagcggac tgcaaatatt
     3241 attgggtgca ttattgatga tatggctgca caggagatag cacggcgttc tcgccgtact
     3301 ccccgaatag ttaacaggct tgtaaaacgt gtacttgatt ttgcgatagt tatgaaaaaa
     3361 gggtcaattg accttgacat cgcacgatat gctttggaca ggcttcacat tgattcttta
     3421 gggcttgatg acatggaccg aaagatactg acaaccatca ttgataaata cgatggcgga
     3481 cctgttggca taaagacaat tgccatctca ataggtgaag agataaccac gctggaagat
     3541 ttttacgaac catacctggt acagattggc atgctgaagc gaactccccg cggcagggta
     3601 gcagcacgtc tggcatatga acatctgcag cttccctaca aaggcatgga tgaatcaagc
     3661 ctgttttagc aattgaggca ataccacata ccatccttct attacaaatc cacaatcaga
     3721 aacgtattac ttttgtagct ttcattaagg tttcagtgat ggtgtacatg ttggatatac
     3781 tgccaacttt caattgatct ttgcgcttat aaaaatcaag gcatgtgcca cagttaataa
     3841 tctgcacacc tttttgtgca atttcctgca atgcgggcag taccggtgat ccttcagtgg
     3901 ttagaaatat cccggaatta acaaatatca tggtagctgg cagcatattg gcctcattaa
     3961 ttgtgttgat gaataatttt atcaaaagct ggcccagttc ctggtgtccc tgaccaaatt
     4021 cgttggatgt aataaagaaa acaatgtttt cattaacaga ctccattgtt ctgggttgtg
     4081 acacaggttg tgattgtgct tgtgtgcctt tacttaaatg gaggataaag taattgccct
     4141 gttgtgtcgt tgtgacagaa tacccacaat tttctgcaaa gcgagttaca ttgcccaccg
     4201 cggtgtcatt gcttaccagt accgtcagat tatcaggatt atccttttct attgtttcct
     4261 tggttaaaag tactggtttt ggacattcaa gccctcgcgc atccacctct ttcataacta
     4321 tctcccttaa aaaattttta attaagatat tgaccagtaa ctatcgtaca atgtattacg
     4381 ttattttgta gtattaatac ttgatgtaac acattgctat tttaaaccct tgtggtataa
     4441 taattatact tgagtaaatt tttaaaacaa ttttttaaca gggttctgaa tggataaaat
     4501 ttttaaacga gattttaaat acagggaaat accctataac tatacatcat tttctgatag
     4561 ggagataatc ttaaagtatt ttgatgctca gacctgggat ctggtgcagg ctctcagagc
     4621 aaagcggcga acaggcagga gtgcaaagct tctctttgag aatattggcg atattttcat
     4681 tatagacaga aatccctata ttcaatatga tattgtggaa aaccctgcca agctgaaaca
     4741 actgtataaa cgacatcaaa gaaggcttgc aaccgtaaaa gaaggtgcta atggtgatca
     4801 gcaggtcctg gaatttatag caaaaattga aaagcttgat gaagcttttt ttagaaagct
     4861 gaaagaaaca aagaagctgc aggaaaagat ttttaataga ttaaaacata tcactgctgc
     4921 tggcaatatt cattttagcc ccttccatcg tgcatctcac gtaactgatg caacagactg
     4981 gcgtgtggag tatcctgttg ttgtggtgta tcctgattca gtgcatgaaa tacaggaact
     5041 ggtgaaggct gcaaaaaagc tgaatcttgt tattattgca cgtggtggtg gtacaggact
     5101 tactggtggt gcaattccac ttttgcctaa cactatgata attaatactg aaaaattgaa
     5161 caat
//
LOCUS       DAOSZP010000084         5163 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_13438_length_5163_cov_2.954385, whole genome shotgun sequence.
ACCESSION   DAOSZP010000084 DAOSZP010000000
VERSION     DAOSZP010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5163
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13438_length_5163_cov_2.954385"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..416
                     /locus_tag="PK348_02825"
     CDS             <1..416
                     /locus_tag="PK348_02825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65180.1"
                     /translation="MAKKIDQLALAGRPFAEAQGILQGVVSYSNEFKPGDLIREKDEK
                     GRILYPLQESKIFQSDVFSLKQGTTSRCINGFDGMYIFTPLVRYIPGTQIPDKERQTL
                     EQNLFYTKANSLYISLLTRLFEKSKIIRNQKFEAQ"
     gene            464..928
                     /locus_tag="PK348_02830"
     CDS             464..928
                     /locus_tag="PK348_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012389599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HXK65181.1"
                     /translation="MNINAEYVNPFLEAASVVFKQLLNVDLRRGKLVLKEVPQPSHDV
                     AIIIGITGAVTGQVVFSMSLEMVKKIAQTLAPGLSEKDIMNEWKDIVGEVANMITGNA
                     MNLFAYSGKRVEMTTPTVIEGESFTITLIKQTTLGINLYSPFGQLEMNVALK"
     gene            complement(935..1732)
                     /locus_tag="PK348_02835"
     CDS             complement(935..1732)
                     /locus_tag="PK348_02835"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXK65182.1"
                     /translation="MNFLHVLIVCSLLVSQPANKEAILETILSEPKAAKIPMQYKTDD
                     DSTQKKGAGTPDDVLLKQGIDFYNAEFYERALQALESLQKNYPQSPFKDQASLWKAKI
                     FVQQQKYTNALQELSTIPQQSGEYPAALFMTGRVNQAMGKPLDAIEYYTRVAGLYPDQ
                     DFSDDALILVAQLYISQKKGNAALSALLKVIKQYSDRETVDDAYYWLGRILSTDPNLK
                     DVELARKVYTIFLKRAQDPAYNAFYSSPLKARVADELKRLDATYFPE"
     gene            complement(1729..2355)
                     /locus_tag="PK348_02840"
     CDS             complement(1729..2355)
                     /locus_tag="PK348_02840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65183.1"
                     /translation="MNPIASILQTIVRMINRMIDSIDIRTQQSIKEGFFFLVFLLCIG
                     AVFLGYHLGKQSAKPAGKPYAEITNDVFDLDIKIKKEPGSFQSMLEGALMEQRQQLSD
                     NKLTYPTKEDEPMTMPAKPIDIPKDIKEIQKPLSGLDDTKPVEPINPDYTPAVVKPVD
                     SMKRDKEKPVASPLKPQSEVDTEKKETIMPKGTKELKPMDIRDKVFEQ"
     gene            complement(2372..3238)
                     /locus_tag="PK348_02845"
     CDS             complement(2372..3238)
                     /locus_tag="PK348_02845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65184.1"
                     /translation="MKLHYRRYVFIFFLAIALALTLSSLSVPVGNKVIQYYNNVEINW
                     TDQIIVAKATGKPFVSESGTPLDTSTYEILSVNNARSRAIEEAKDRATESLITALQII
                     QVDPIRSLGDCIQSDDYTQMQLGMYPNDIKYSFKPDGAIATQCTAILPMGRLLKLLPE
                     ALPAKDIPQLPGKLPSTDYSGLIVDTRGQHFSPMLFPSLFAEDGTEIFGKNFIDSRDA
                     YVYGTVAYCFSDDVAMHHKKAGKNPYYCAALRLYNRCPVIANTDLKKILASSVTRNNL
                     KKGNIIIIIDRD"
     gene            complement(3299..4978)
                     /locus_tag="PK348_02850"
     CDS             complement(3299..4978)
                     /locus_tag="PK348_02850"
                     /inference="COORDINATES: protein motif:HMM:NF014075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF115 domain-containing protein"
                     /protein_id="HXK65185.1"
                     /translation="MENFTITIAKSGHKTLHYTNGQQTIRIHSAYDPVKEAQRIADQC
                     NPKRASIIIVCGLGLGYHVQALKDKFPGLTIIVIEKDKNLIHLVKQEFPEVFTCAHIV
                     NNEEEIATVLESIDIRSFRGTALLVHRPSYSLHPEFYDALIANIHKQISSRISDLLTR
                     FEFEELWVKNILLNAKQMHTALPVKSLFGKFKGIPGIIVSAGPSLINSLDALSQAYEK
                     ALIVCVDTAYKVLERHGISPHIVMTLDAQTHSIKHFLGITQKPLLLADVVSSPKVTRL
                     IEKKLFSTTAKYYTAPDGRIQRESTPLMEWIQNFTGQIGDIQSGGSVATSAFDLLLTA
                     GCSAIVLVGQDLAYTGREIHSRGTHHNDDWLPAINRFKNLDTINQNVIRKRKIKYVPS
                     NSGSTVITDFVLDLYRSWFEDSAKRVPIPVYNTSTGGAVIANTTFTPLQALVQKWKKP
                     PVSPQEIISHELSHKTPINTQSLFRKFSRLHHRLKKLQTAQGEDAANLYAMLDNEDLD
                     TLCSPFMRKTLFYIARHNLEQQKIDMLIKDAAQAAARELLKIFAKLEDSLI"
     gene            complement(4984..>5163)
                     /locus_tag="PK348_02855"
     CDS             complement(4984..>5163)
                     /locus_tag="PK348_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65186.1"
                     /translation="YKDHVSITYLANLLCGVESRKYFYYYCEDEICRQFKIETQDAFI
                     KLHKLCMDTYNKIAQ"
BASE COUNT         1402 a          996 c         1138 g         1627 t
ORIGIN      
        1 aaatggcaaa aaagattgac cagcttgcac ttgcaggcag gccatttgca gaagcccaag
       61 ggatattgca gggagtggtg tcatattcca atgaatttaa gccaggcgac ctcataaggg
      121 aaaaagatga aaaggggcgg atactgtatc ctttacagga gtcaaagata ttccaatcag
      181 atgtttttag tttaaagcaa ggaacaacgt cgcggtgtat taacggattt gacgggatgt
      241 atattttcac gcctttggta cgatatatac cgggcacgca gattcctgat aaggagcgcc
      301 agacactgga acagaacctt ttttacacca aagctaattc tttatatata tcactgttaa
      361 caagactgtt tgaaaagtcg aaaataataa ggaatcagaa gtttgaggca cagtagaagg
      421 tacaaggtgg ttttaatttt tgttacacag caaggggtac tgtatgaata ttaatgctga
      481 atatgttaat ccgttcttag aggcagcaag tgtagtattc aaacaacttc ttaatgttga
      541 tttaagaaga ggaaagctgg tattgaaaga agtaccacag ccatcacatg atgttgctat
      601 tatcattggt attaccggag cggtcacagg tcaggtggtt ttcagtatga gcttagagat
      661 ggtaaaaaag attgcccaga cccttgctcc gggattatct gagaaagata taatgaatga
      721 atggaaggat attgttggtg aagttgccaa catgattacc gggaatgcaa tgaacctttt
      781 tgcgtattca ggaaagcgcg ttgagatgac aacacctacc gtcattgaag gagaaagttt
      841 tactatcacc ttaataaaac agaccactct ggggattaat ctttacagcc catttgggca
      901 gctggaaatg aacgttgcat tgaaatgaat agtgttattc agggaaatag gtcgcatcta
      961 aacgcttgag ctcatcagca actcttgctt tcaatggtga tgaataaaag gcattatatg
     1021 caggatcctg agcacgcttc aggaatattg tatatacttt gcgtgccagt tcaacatctt
     1081 tcaaattagg gtcagtactt aaaatcctgc ctaaccagta gtatgcatcg tcaactgttt
     1141 cacggtcact gtactgcttg ataactttca gtaatgcact taacgctgcg ttgccttttt
     1201 tttgcgatat atagagctgt gcaaccagaa tcagcgcatc gtcagaaaaa tcctggtcag
     1261 ggtataatcc tgctactctg gtgtagtatt cgatagcgtc cagtggcttg cccattgcct
     1321 ggtttaccct gcctgtcata aaaagtgccg caggatattc cccggattgt tggggtatgg
     1381 tcgatagttc ctgtaaggca tttgtatatt tctgctgctg tacaaaaatc tttgctttcc
     1441 ataaacttgc ctgatcttta aagggacttt gagggtaatt tttttgcagg ctttccaggg
     1501 cctgtagagc ccgttcatag aattcagcat tataaaagtc aatgccctgt ttcagcaaca
     1561 catcatcagg agtacctgct cctttctttt gagtagaatc atcgtcagtt ttatattgca
     1621 tggggatttt agcggctttt ggttcagata aaatggtttc cagtatagct tctttatttg
     1681 ctggctgtga taccagtaac gagcatacta tgagtacgtg taaaaaattc attgctcaaa
     1741 caccttatca cgtatatcca tgggcttaag ttctttggtg ccttttggca taatggtttc
     1801 ttttttttca gtatcaacct ccgattgtgg cttaagcgga gaagcaacag gcttttcctt
     1861 gtcgcgtttc attgaatcaa caggctttac cactgctggt gtataatcgg gattgatggg
     1921 ttcaaccggt ttagtgtcat caagccccga cagaggtttt tgtatttcct tgatatcttt
     1981 aggaatatca ataggttttg caggcattgt cattggttca tcttcttttg tggggtaggt
     2041 tagtttattg tccgataatt gctggcgctg ttccatcaat gcaccttcaa gcatggactg
     2101 gaatgagcct ggttccttct ttatcttgat atccaggtca aatacgtcat ttgttatttc
     2161 tgcatacggt ttgccagctg gctttgctga ctgtttgccc agatgatatc ccagaaatac
     2221 tgcaccaata catagaagaa agacaaggaa aaagaagccc tcttttatgg attgctgtgt
     2281 tcgaatatca atagaatcaa tcatcctgtt gatcattcgt acaatagttt gtaatatgga
     2341 tgcaataggg ttcatatgat tgtgcttctt attagtctct atcaataata ataataatat
     2401 tgcctttttt caagttattt cttgttacac tgcttgcaag gatttttttt aagtcagtat
     2461 tggcaataac cgggcacctg ttataaagac gcaatgctgc gcagtagtat ggattcttac
     2521 cagctttttt atgatgcatc gcaacatcat cgctgaaaca ataggcaaca gttccataca
     2581 cataggcatc acgggaatca ataaaattct tgccaaatat ttcggtacca tcttcagcaa
     2641 atagcgacgg aaaaagcatg ggtgaaaagt gctgtcctcg tgtatccaca atcagccctg
     2701 aatagtcagt tgatggcagt ttaccaggca actgcggtat gtccttagct ggcaatgctt
     2761 caggcaaaag ttttaaaagc cttcccatcg ggagtatagc agtgcattga gtggcaatag
     2821 cgccatcagg tttaaagctg tatttgatat cattggggta catgcccaac tgcatctgcg
     2881 tataatcatc actctgaata caatcgccca gtgaacgtat ggggtcaacc tgtattatct
     2941 gcagtgccgt tataagagat tccgtagccc tgtctttagc ttcttctatt gccctgctgc
     3001 gtgcattgtt cactgacaat atctcatagg ttgaggtatc aagtggtgtg ccgctttcag
     3061 aaacaaaagg ctttcctgtt gcttttgcca caattatctg atcggtccag ttgatttcta
     3121 cattgttgta gtactggata actttattgc ccacaggcac tgatagtgaa gaaagggtca
     3181 atgccagagc tatcgccagg aaaaaaataa aaacataccg cctgtagtgt agtttcatag
     3241 tatgcaacat agtagtttcg tttttaaagt tatcggacaa aatgacactg aaatatagtc
     3301 aaattaatga gtcttcaagt tttgcaaata tcttcaaaag ttcccgtgct gctgcctgcg
     3361 ctgcatcttt gattaacata tcaatcttct gctgttcaag gttatggcga gcaatataga
     3421 acaatgtttt gcgcataaag ggggaacaca gcgtgtccaa atcttcatta tcaagcattg
     3481 catacaggtt tgctgcatct tctccttgtg cagtttgcaa tttttttagc ctgtggtgca
     3541 gacgtgaaaa tttcctgaaa agggattgtg tattgatggg agttttgtgc gatagttcat
     3601 gggatataat ctcctgtgga ctaacaggag gctttttcca cttttgaacc agagcctgca
     3661 gtggtgtaaa tgttgtattg gcaatcactg ctccgcccgt actggtgttg tacaccggta
     3721 tgggtactcg ttttgctgag tcctcaaacc agctccggta caaatccagg acaaaatcgg
     3781 tgatgacagt gctgccactg ttggatggga catattttat tttgcgtttt cggatgacat
     3841 tctggttgat agtatcaaga tttttaaagc ggtttatggc aggaagccag tcatcattat
     3901 gatgggtgcc ccggctgtga atttccctgc ctgtgtaagc taaatcctga cccactagca
     3961 ctattgcact gcacccggcg gtgagtagta agtcaaacgc actggtggca accgaacccc
     4021 ctgattgaat atcgccaatc tggccggtaa aattctgaat ccattccata agtggtgtag
     4081 attcacgttg tatcctgcca tcgggggcag tgtagtattt tgcggttgtg ctgaataatt
     4141 ttttttcaat caaacgggtt actttagggc tgctgacaac atccgctaac agaagtggtt
     4201 tctgtgttat gcccagaaaa tgttttatgc tgtgtgtttg tgcatcaagc gtcataacta
     4261 tatgggggct tataccatgg cgttccagca ctttatatgc agtgtcaaca caaacaatta
     4321 aagctttctc atacgcctgt gacagcgcat ccaggctgtt aattaatgaa ggcccggctg
     4381 atacaatgat acccggtatg cccttaaatt tgccaaatag cgactttacc ggtagtgcgg
     4441 tatgcatctg ttttgcattg agcaatatgt ttttaaccca tagttcctca aattcaaagc
     4501 gggttaaaag gtcactgatg cgagagctta tctgcttgtg aatgtttgct attagtgcat
     4561 cataaaattc agggtgcaac gaatacgatg gccggtgtac cagcagggcg gttcctctga
     4621 acgacctgat atcaattgat tcaagcactg tggctatctc ttcttcatta ttgactatgt
     4681 gcgcacatgt aaatacttca gggaattcct gcttaacaag gtgtataagg tttttgtctt
     4741 tttcaataac tatgatagtc agtccgggga atttatcttt taaggcctgt acgtggtatc
     4801 ccagtcccag cccgcaaacg ataatgattg aagcacgttt agggttacac tgatcggcta
     4861 tgcgctgcgc ttctttaaca gggtcgtatg ctgaatgaat gcggattgtt tgctgaccat
     4921 tagtatagtg cagtgttttg tggccacttt ttgctatggt tattgtaaag ttttccatga
     4981 gatttactgc gcaattttgt tataggtatc catgcagagt ttatgcagtt tgatgaaagc
     5041 atcttgtgtt tctattttaa attgtcgaca tatttcatct tcacaatagt aatagaaata
     5101 ctttcttgat tcaactccgc acaaaaggtt agctaaatag gtaatagaaa catgatcttt
     5161 ata
//
LOCUS       DAOSZP010000085         5161 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_13443_length_5161_cov_3.606150, whole genome shotgun sequence.
ACCESSION   DAOSZP010000085 DAOSZP010000000
VERSION     DAOSZP010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5161
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13443_length_5161_cov_3.606150"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(85..1470)
                     /gene="asnS"
                     /locus_tag="PK348_02860"
     CDS             complement(85..1470)
                     /gene="asnS"
                     /locus_tag="PK348_02860"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015225265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HXK65187.1"
                     /translation="MKRMRIVDCLKSDIPIDDCIVAGWVRTKRESKENIFIEINDGSC
                     LSNLQVVVGVGDSSFDIAQRLHTGASVYIQGSLVPSPAVGQTWELKPNIITVFGDADP
                     ESYPLQKKRHSFEFLREIAHLRPRTNTMGAIMRIRNQVSYAIHTFFQQKGFLYVHTPI
                     ITTSDCEGAGEMFQVTTLDLHNLPMVNGAIDYTKDFFGAKAALTVSGQLEGELLAMAL
                     GDIYTFGPTFRAENSNTTRHLSEFWMVEPEMAFATLEDDCSLAEEFLRFIFKWVLEKC
                     QEDMQFFNERIKPGIIQNLELIVNSSFEIVTYTEAIEKLLQSKESFEFPVHWGMDLQT
                     EHERWLTEKYFKKPIIVIHYPKTIKAFYMKLNDDNNTVRAMDVLVPGVGEIIGGSERE
                     DRYDVLITRMKEMGLAPDDYWWYMDIRKFGSAPHAGFGLGFERAIQFITGMQNIRDVI
                     PFPRTPGNARF"
     gene            complement(1522..2751)
                     /locus_tag="PK348_02865"
     CDS             complement(1522..2751)
                     /locus_tag="PK348_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015947228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HXK65188.1"
                     /translation="MKISCIELYHVSIPLRHTFWPTWIPGYPQTHNRFTLIKLITDDG
                     IEGYSAGTAMGKEREGLGDLLAGYIMGTDPNDIDRVQSLLKQAGYLGWRNFWIEPAFW
                     DIIAKSKGKPVYELLGGNARPIEVYCSTGEMHDPHKRVDEILKLTERGFKTFKLRVKN
                     KELKDDIRHIEIISKGVGNKVKLGVDANQGWPVSIVDRVPDWDLKRAKEFVRACEANN
                     IVWLEEPLDSRDYDGNAALKRFAKKVKISGAELNYGWDEIKIMFEKDCFHIYQPDATF
                     AGGIAQVKKVIDTCHEKKREFSPHTWTNGIGFYINWNMVLSDTKNKLPLEYPLEEPSW
                     IPEFRDGIIEPIIPDKNAMLQPFTRPGLGFEIDKKLLSKYGKRYFRMTEFGLKIKVIR
                     EKGIKEALALKKRKEGQ"
     gene            3113..3907
                     /locus_tag="PK348_02870"
     CDS             3113..3907
                     /locus_tag="PK348_02870"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HXK65189.1"
                     /translation="MRFIFAALLFFLSVSPLYASNKVYVLVYHTFMGKKIKYDISIQE
                     FQKQLLDLRSHGFSFISFDDIKYRKVSGDKNILITIDDGHHTVLDAYYSVLKPLGIKP
                     LLGINTFIIGKKPYVLTWEELQQLTREGCAIASHGYFHLFINDELYTKNYRYFKLEVF
                     ESKKMLEEKLGITVDTFVYPFGVVTDIGKQALSQAGYTYAFTIKWGPLSLPLAHNTLE
                     LPRYMYQNNWNSIANAIIYKNKKRTPAREPEKILVSTTHQLKGIHQ"
     gene            complement(3873..>5161)
                     /gene="gltA"
                     /locus_tag="PK348_02875"
     CDS             complement(3873..>5161)
                     /gene="gltA"
                     /locus_tag="PK348_02875"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HXK65190.1"
                     /translation="ATRCLQCKRPTCVEGCPVNINIPGFIKAVKEGNPLEAFAVIKQT
                     NSLPAVCGRVCPQEDQCEKLCVLAKKGDPVAIGNLERYVADYEREKQHYTFTKGETVK
                     GKRVAIIGSGPAGLTAAGDLARMGYSVTVFEALHKAGGVLVYGIPEFRLPKDIVQYEV
                     DALKAMGVTIELSRVIGTAETVNDLLSSGFDAVFVATGAGLPLFLDIPGENLQGVYSA
                     NEYLTRSNLMKAYRFPEYDTPIVKGEKVAVVGGGNVAMDSARTALRLGSKEVYIIYRR
                     SKKEMPARIEEVERAEEEGVIFKLLTNPVRFIGDEKGRVKAVECIQMELGEPDASGRR
                     SPIPVKGSEFIIDIDVAIIAIGNGPNPLIPKTTPGMDTTKRGNIIADPETMKTSKRGV
                     FAGGDIVTGAATVIQAMGAGRKAAKAIDEYLSTGVW"
BASE COUNT         1452 a         1237 c          882 g         1590 t
ORIGIN      
        1 ctctttaaaa tgctgcataa tatattgttt taactcaacg cctgcatgat cattaccaat
       61 tgctattttc atagtatcca cctgctagaa tcgtgcattt cctggtgtac ggggaaatgg
      121 tattacatca cgtatattct gcataccggt aataaactga attgcacgct caaaccccag
      181 gccaaaacca gcatgtggtg ctgaaccaaa tttgcgtata tccatatacc accagtaatc
      241 atcaggggca agtcccattt ctttcatacg tgttatcaac acatcgtatc tgtcctcgcg
      301 ctcacttcca ccaataatct cgccaacacc cggcaccagt acatccattg ctcttacagt
      361 gttattatcg tcattgagct tcatataaaa agcttttatt gtctttgggt aatgtattac
      421 tattattggc tttttgaaat atttttcagt taaccagcgt tcgtgttcag tttgcaggtc
      481 catcccccag tgtaccggaa attcaaatga ctctttggat tgcagtaatt tttcaatggc
      541 ttctgtgtat gtaactatct caaacgaact gttaacaatt aattctagat tctggataat
      601 gcccggcttt atccgttcat taaaaaactg catatcctcc tggcatttct caagcaccca
      661 tttaaaaata aaacgcaaaa attcttctgc caatgaacaa tcatcttcaa gggttgcaaa
      721 tgccatctca ggctcaacca tccagaattc ggacaaatgg cgcgtggtat tggaattctc
      781 ggctctgaat gtaggcccaa atgtatagat atcaccaagc gccatggcca gcaactcccc
      841 ttcaagctgt ccactaaccg ttaaagctgc tttagcacca aaaaagtcct tggtataatc
      901 aattgcacca ttgaccatcg gcaaattatg taaatcaagt gtcgtcacct gaaacatctc
      961 accagcgcct tcacaatcgc tggtggttat aatgggtgta tgcacataca aaaatccctt
     1021 ttgctggaag aatgtatgaa tggcatacga tacctgattg cggattcgca tgatggctcc
     1081 catggtgttt gttcgcgggc gcaggtgtgc tatctcacgt aaaaattcaa atgaatgcct
     1141 ctttttctgc aagggatagc tttcagggtc agcatcgcca aacacagtaa tgatattggg
     1201 ttttagttcc catgtctggc ctacagcagg ggatggcacc agactgccct gtatatacac
     1261 acttgcaccc gtatgcagcc tttgggctat atcaaaggaa ctatcgccca cacccaccac
     1321 cacctgcagg tttgacaggc aggagccatc attaatttca ataaagatat tttccttgga
     1381 ttcccgtttt gtgcgcaccc atccagctac tatgcaatca tcaataggaa tatcactctt
     1441 caaacaatct actattcgca ttcgtttcat atttttcttt ggtgcctttc acgtacccat
     1501 caattatttc atctataaaa attattggcc ttccttgcgt ttctttaatg ctaaggcttc
     1561 ctttattcct ttttcacgta tcaccttaat ctttaaacca aactcagtca ttcggaagta
     1621 ccgctttcca tattttgata gtaatttttt gtcaatctca aaaccaagtc ccgggcgtgt
     1681 aaatggctgc agcattgcat ttttatccgg aataattggc tcaatgatgc catccctgaa
     1741 ttcaggaatc catgaaggtt cttctagtgg atattccagt ggcagtttat ttttagtatc
     1801 agaaagtacc atattccagt taatataaaa tcctatgcca ttggtccatg tatgcggtga
     1861 aaactctcgc tttttttcat ggcaggtatc aatgaccttt ttgacctgcg caatccctcc
     1921 ggcaaacgtt gcatcgggct gatatatatg gaaacagtcc ttttcaaaca ttatctttat
     1981 ttcatcccat ccgtagttga gttctgcgcc tgaaatctta accttttttg caaatcgctt
     2041 taatgccgca tttccatcat aatcacgtga atccagcggt tcctcaagcc atacaatgtt
     2101 gttggcttca catgctcgca caaactcttt tgcacgcttt aaatcccagt caggaaccct
     2161 gtcaactatt gaaacaggcc atccctgatt ggcatccact cccagtttta ctttgttacc
     2221 aacacctttt gatataattt caatatggcg tatgtcatcc ttaagttctt tattttttac
     2281 ccgcagttta aatgttttaa atccgcgctc agttaacttt aagatttcat caacacgctt
     2341 gtgtggatca tgcatttcac cggttgaaca gtatacctca atagggcgtg cattgccacc
     2401 caacagttca tagactggct ttcctttact cttggcaata atgtcccaga atgccggctc
     2461 tatccagaaa tttctccacc ccaaataccc tgcctgtttt aaaaggcttt gaacacggtc
     2521 aatatcattt ggatcagttc ccatgatata cccggctaaa agatctccaa gcccttcacg
     2581 ctctttaccc atagccgtcc ctgctgaata cccttcaatg ccatcatccg ttataagctt
     2641 tatcaacgta aatcggttat gtgtctgtgg atacccggga atccacgttg gccagaatgt
     2701 atggcgcaat ggtatagaaa catggtataa ttcaatacag cttattttca tggctaaccc
     2761 ccattcgtat agtatgttaa atacgttaac agatagtaat gtcccgtcaa tcacaattca
     2821 attgctttac aatattctcc tactataatt gtaaaattac aaacttttat gttgacaggc
     2881 atggtttgat aattgtttta tagttataat ttatttgtac cgatagttac tgttaataca
     2941 tcaacatatc tttgtgtata tcacaataaa attttcgtaa aagattatgc agtgaacgtt
     3001 gagtgcgtag aaaaaatatt ttcagttgat agaacatgat tacttatccc gattaattaa
     3061 aatgaaaaac aacaaaaaca aaggatacca aaactatttt cgaggaaaac ccatgagatt
     3121 tatttttgct gccttattat tcttcttatc agtttctcca ctctatgcca gcaataaagt
     3181 ttatgtactt gtttatcata cctttatggg aaaaaaaatt aaatatgaca tatcaattca
     3241 ggaatttcaa aaacaactgc ttgatttgcg ttcgcatgga ttttctttta tatcatttga
     3301 tgatatcaaa tacagaaaag ttagcggtga taaaaatatc cttatcacca tcgatgatgg
     3361 ccaccacaca gttcttgatg catattattc agtattaaag cctttaggca taaagccatt
     3421 gctgggcatt aatacattca ttattggtaa aaagccttac gttctcacct gggaagaatt
     3481 acagcaatta accagggaag gatgtgcaat agcttctcat ggttactttc atttgtttat
     3541 caacgatgag ctatatacta aaaactatcg ctattttaaa ctagaagttt ttgaatcaaa
     3601 aaagatgctg gaagaaaaat taggcattac cgttgatacc tttgtgtatc cctttggcgt
     3661 tgtaactgat attggaaaac aggcactgtc acaggcgggc tatacgtatg cattcaccat
     3721 taagtggggc cctctttcat tacccctggc acacaatact ctggagcttc ctcgctatat
     3781 gtaccagaac aactggaatt ccattgccaa tgcaattatt tataaaaata aaaaacgcac
     3841 accagcaagg gaaccggaaa agatactggt ttctaccaca caccagttga aaggtattca
     3901 tcaatagcct ttgccgcttt tctgccagct cccattgcct gaataacggt tgcagcaccg
     3961 gtaacaatat caccaccggc aaacactcca cgtttactgg ttttcatagt ttccgggtca
     4021 gcaataatat tgccacgttt tgtagtatcc atccccggtg tagtttttgg tattagggga
     4081 tttgggccat tgccaatggc aatgattgct acatcaatgt ctatgataaa ctctgaccct
     4141 ttaacaggta tggggcttcg ccttccggaa gcatcaggtt cacctaattc catctgaata
     4201 cattcaaccg ctttaaccct gcccttttca tcaccaataa accgcacagg attggtcaaa
     4261 agcttaaaga taacgccttc ttcttcggcg cgctccactt cctcaattcg agcaggcatc
     4321 tcttttttgg aacgacggta aataatatat acttctttac tacccaaacg cagtgctgta
     4381 cgtgctgagt ccatagcaac gttgccacca ccaacaacag ccaccttttc acctttaaca
     4441 attggcgtat catactcagg gaagcgatat gctttcatca aattggaccg tgttaaatat
     4501 tcattggcac tgtacacacc ctgaagattt tcgcccggta tatccagaaa taatggcaat
     4561 cctgcgccag ttgccacaaa caccgcatca aaaccggaag atagcaagtc attgacagtt
     4621 tctgcagtgc ctatcaccct gctcagttct atggtaacac ccattgcttt aagagcatct
     4681 acctcatact gtactatatc ttttggcagc ctgaactcag gtattccata taccagaact
     4741 ccaccagctt tatgcaatgc ctcaaacacg gtaacactgt accccatacg ggccaaatct
     4801 cccgcagccg ttaaccccgc tggccccgaa ccaatgatgg caaccctttt tccctttact
     4861 gtttcacctt tcgtgaatgt ataatgctgt ttttcgcgtt cataatcggc aacgtaccgt
     4921 tccaggttac cgatagcaac cggatctcct ttctttgcca gaacgcacaa cttttcacat
     4981 tgatcctcct gggggcaaac cctgccacac actgccggca gcgaatttgt ctgctttata
     5041 acagcaaatg cctccagtgg attgccctct ttcaccgctt tgataaatcc cggaatatta
     5101 atattcacag gacacccttc cacacaggta gggcgcttac actgcaggca acgggttgct
     5161 t
//
LOCUS       DAOSZP010000086         5150 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_13474_length_5150_cov_3.273798, whole genome shotgun sequence.
ACCESSION   DAOSZP010000086 DAOSZP010000000
VERSION     DAOSZP010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5150
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13474_length_5150_cov_3.273798"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            208..1026
                     /locus_tag="PK348_02880"
     CDS             208..1026
                     /locus_tag="PK348_02880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65191.1"
                     /translation="MFLSIFLLPFSSQAQVNSGDFGLILNGGVGTGSSMYGVILNTTN
                     DSSSTLGVGPGSSFNAGFMANFNIIAIHSQLYYVTLKDLEWEQEVSGVDRKFKTEGSG
                     HFWTWDGTIGIKALTEEGDMGYTHVYVGFRIWKVLREEDKKTVDGVSNPLYPPVKQEL
                     TGNGFIVGIRDFSTLPLGSISLALQTGLWLYKAPLSTFKQDGTEIDTTADQNIGFGFE
                     IGGGLAFEDIGLSTIVGLRMDVQATEIKLPLNIDAVAGSGYAQFFFAVSKEFGF"
     gene            complement(1089..>1569)
                     /locus_tag="PK348_02885"
     CDS             complement(1089..>1569)
                     /locus_tag="PK348_02885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65192.1"
                     /translation="NTLKPDVPNAEDFKLNGVAIEGEYNLNLGGMLLGFSLEYAYLSY
                     KDDKAYDYGFLSPMVTLKFLAPGGFYIGPGVSARYLMDSYLPKGQDEPDSEVDLWVNG
                     VAGFMTPIAEGIYLDLQARFGWNLTQNQFEDIFTKIDKNYDMAFYVGVGTRTRSTGI"
     assembly_gap    1570..1579
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1794..1958)
                     /locus_tag="PK348_02890"
     CDS             complement(1794..1958)
                     /locus_tag="PK348_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020249087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HXK65193.1"
                     /translation="MQELIKEGCFLKRHGANHDIYVNPSNGKKAPVPRHAEIKDSLCK
                     LIRKQLGIDK"
     gene            complement(1946..2176)
                     /locus_tag="PK348_02895"
     CDS             complement(1946..2176)
                     /locus_tag="PK348_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017839951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HXK65194.1"
                     /translation="MTKTFTLEYWIDEGWYVGRLLEVPGVFSQGETLEELIVNIREAY
                     KLMVEEVEPVYIETKRKEIEVEVSKEKNLFRS"
     gene            complement(2277..2567)
                     /locus_tag="PK348_02900"
     CDS             complement(2277..2567)
                     /locus_tag="PK348_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65195.1"
                     /translation="MRISDGLSIITLPQTTAKPARTVMVWPVYDDSRITPVKGVARRL
                     ENNIQYIKPTPDEHARLLSQVQENPEPLYSNTARIQKSMSAVIPGTFFDAIA"
     gene            complement(2632..2870)
                     /locus_tag="PK348_02905"
                     /pseudo
     CDS             complement(2632..2870)
                     /locus_tag="PK348_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004494163.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
     gene            complement(2900..4684)
                     /locus_tag="PK348_02910"
     CDS             complement(2900..4684)
                     /locus_tag="PK348_02910"
                     /inference="COORDINATES: protein motif:HMM:NF024598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HXK65196.1"
                     /translation="MKSLALSVCLLVTSLSYAWQPLLYQATNGKLYYTGKPYHQGLPD
                     TIHIKDIQVIKNTFYISTREHGLFKRILGDSSWQHITPRDAHIRTTNGLLPTYRSISA
                     FCVDGSDPSILYVATKYSLYCSEDGGTSWQSVALTGLPANACITALYAKGSTLIVGTS
                     WNGVYRYTGTRFINISNGLPRKHYSDSIVFIEEVYRVSPEFCITTYTNEVYSYNGKEW
                     KKRLSGHTGTFWGGSINGKVVVVDNDTVVTLTGQKEIHQELSPIQAHCVLFLEGTQYV
                     FVKAIEEQKDTVRGLYAGVPSSWKVVKELVAVAKKAGYNALVFDIKDDYGNVYVSEPT
                     AESIGALRKGLPPEEVCTYVHNHTMKAIARMVVFKDERLFKAFDTKYAIKDSATKAAW
                     RGNPKEYWVDPYAEFVHSYNIAIAKEAQRAGFDEIQFDYIRFPTDGPVHRCVFTYKPP
                     NGIYKYEAIAQFLRSARQALHVPISVDVYGFTVWYEFGQWLGQDIEAMSSIVHVLCPM
                     VYPSHYGRKFFEGNIDPGDYYAIVIESNRRALMKSHAVVRPYLQAFNMLSPGWGYTYI
                     QRQIEACTDSGVSGYIWWNAGRDYGVIK"
     gene            complement(4665..>5150)
                     /locus_tag="PK348_02915"
     CDS             complement(4665..>5150)
                     /locus_tag="PK348_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HXK65197.1"
                     /translation="ASENEKKLHYVEQLLKHHPNDRVLIIGQYIDQLQKIQERFSFPL
                     ITGSTPVKEREELYKQFKQGAITRLIVSKVANFSIDLPDANVAIQVSGTFGSRQEEAQ
                     RLGRILRPKPGDNTAHFYSLITAHTVEENFAHNRQLFLTEQGYTYIIMNESMFNEKFS
                     T"
BASE COUNT         1474 a         1093 c          945 g         1628 t
ORIGIN      
        1 ataatatatt ctgattgttt atgtgttctc agcatgatta attttttacc aaccatcacg
       61 cagggaacat gttagtacgt aaaggacaat ttttatagtt ttaaaaaaac atgtttcact
      121 ttttgggtca tcattgatga ccaataagaa tattttttcc cagggaggtt atatgttaaa
      181 aaaactgttc tagcattttg tatttcagtg tttctcagca tttttctatt accgttttca
      241 tcacaggctc aggttaacag tggtgatttt ggattaatcc taaatggtgg cgttggtaca
      301 ggttcttcaa tgtacggcgt tatactcaat accacaaacg attcaagttc aacattaggg
      361 gtaggtcctg gcagcagttt taatgctggt tttatggcaa attttaacat cattgcaatc
      421 cattcacaat tgtactatgt aacactaaaa gatttagaat gggaacagga agtttcagga
      481 gtagatcgta aattcaagac tgaaggttct ggccatttct ggacatggga tggcaccata
      541 ggaattaaag cattaaccga agaaggcgat atgggatata cacacgttta tgtggggttc
      601 cgtatatgga aagtactccg ggaagaagat aagaaaactg tagatggtgt atccaatcct
      661 ttatatccac cggtcaagca ggaattaact ggtaatggat ttatagttgg tatccgtgat
      721 ttttcgacat taccgttagg atctatctca cttgcattgc aaacaggttt atggctttac
      781 aaagcccctt taagtacctt caagcaagat ggtactgaaa tagacacaac agccgatcag
      841 aatattggct ttggatttga aattggcggt gggctcgcat ttgaggatat tgggctttcg
      901 accatagttg ggttacgtat ggatgtacag gccactgaga ttaaacttcc attaaatata
      961 gatgcagtag caggttcagg atatgcacaa ttcttctttg ctgttagcaa ggaatttgga
     1021 ttctaataaa taaaaaaaca caggcaggga ttaacctgcc tgtgtttata aacaatatgg
     1081 tacttacatt atattccagt tgagcgggtg cgggtaccaa caccaacata gaaagccata
     1141 tcatagtttt tgtcaatttt cgtgaaaata tcttcaaact ggttttgcgt cagattccag
     1201 ccaaagcgtg cctgaaggtc taaataaata ccttctgcaa tgggtgtcat aaagcctgct
     1261 acaccattta cccacaggtc aacttcacta tcaggttcat cttgaccctt tggtaaatag
     1321 gaatccatca aatacctggc tgaaacaccc gggccaatat aaaaaccacc aggagctaag
     1381 aacttgagtg ttaccatggg gcttaaaaag ccataatcgt atgctttatc atctttatat
     1441 gacaaatatg catattctaa agaaaatcca agaagcatac cacctaaatt aaggttatat
     1501 tcaccttcaa tagctacgcc atttagtttg aaatcctctg cattaggtac atctggcttc
     1561 aatgtattcn nnnnnnnnnc ctgtgcctat agccctgttg tcacacatgc aacagcagca
     1621 acaagaatac acaacgctaa cttcttaaac atcgtaaaac ctccttactt ttgatttttt
     1681 attatgttgc ttttttgata cacaaactac aaatgcactt tattgtttat atatatactg
     1741 gcaacaaaaa ttgcaagctt tattttttat ccaaagttat tgaaaaaaat ttatcatttg
     1801 tctattccta attgctttcg tataagttta catagactat cttttatttc tgcatgacgt
     1861 ggtactgggg cttttttccc attagaagga ttaacatata tatcatgatt ggctccatgt
     1921 ctttttaaaa aacaaccttc ctttattaac tcctgaataa attcttttct tttgaaactt
     1981 caacctcaat ttccttcctc ttggtttcaa tatatactgg ttcaacttcc tccaccatta
     2041 gtttatatgc ttcacgtata ttgactatta attcttcaag tgtttccccc tgactaaata
     2101 ctcccggtac ctcaagcaat cttccaacat accatccttc atctatccag tactcaagtg
     2161 tgaatgtttt ggtcatatac tttttatcct cgtaatataa taagtgttta atactgatgg
     2221 attcagaaat aaagataaaa ttaatttcac taaatgtaaa gaaaaaataa cgatatttac
     2281 gcaattgcgt caaaaaaggt gccggggatg actgctgaca tgcttttctg gattcttgct
     2341 gtgttgctgt acagtggttc ggggttttcc tgtacctgcg atagcaaccg tgcatgctca
     2401 tcaggagtgg gctttatgta ttgaatgtta ttctctaacc tgcgtgcaac gccttttacc
     2461 ggtgtgatgc gtgaatcatc atataccggc cacaccatca ccgtgcgagc aggttttgct
     2521 gtagtttggg gcagtgtaat aattgataaa ccatcagaga ttctcatgta gccattatcg
     2581 gcataaatta tgaaaatatt gagttaataa cacggggcgc catgtaatta tttatgaaca
     2641 acatacccaa tttcggtacc tgccttccgt tatctctttt aatcctttcc gcaaatcctt
     2701 taccagatgc acatcaccat agtatttctt ccatttcctg atcagatact gttatttcat
     2761 ttgtgatata tgataatgtt gcatattcac taaaatctga cagagacctt cgttcaccat
     2821 ctgctacttt tttttatttc atatacagca ccatcaactc gtagaggaat agtctttggc
     2881 ctactttcct cgtattactc tattttataa caccataatc tcttcctgca ttccaccata
     2941 tatagccact tacacctgaa tctgtgcagg cctctatctg cctctgtatg tacgtatagc
     3001 cccatcctgg tgaaagcata ttgaacgcct gcaggtacgg ccgcaccaca gcatgtgatt
     3061 tcattaaagc cctgcgattg ctttcgataa ctatcgcata ataatcccca ggatctatgt
     3121 ttccttcaaa aaatttcctg ccgtaatgcg aaggatatac catggggcac aatacatgca
     3181 ctattgatga catagcttca atatcctgtc ccaaccactg accaaattca taccatacgg
     3241 taaagccata cacatccaca cttattggca catgaagcgc ctgtctggca gatcgtaaaa
     3301 attgcgctat agcctcatac ttataaatcc cattgggggg tttatacgta aatacacagc
     3361 gatgcactgg cccatcggta gggaagcgaa tataatcaaa ttgaatctca tcaaagcctg
     3421 ccctttgtgc ttctttagca attgcaatat tgtagctgtg cacaaactct gcatacgggt
     3481 caacccagta ttcttttgga ttgccgcgcc atgctgcttt tgtagcacta tccttaattg
     3541 catattttgt atcaaaggct ttaaacaggc gctcatcttt aaataccacc atgcgggcaa
     3601 ttgctttcat ggtatggttg tgtacatacg tgcaaacctc ttctggtggc aggcccttac
     3661 gcaatgctcc aatagattcc gcagttggtt cactcacata cacattgcca taatcatctt
     3721 taatatcaaa taccagagca ttgtaaccag ccttctttgc aacagcaaca agctctttaa
     3781 ctactttcca tgatgaaggc actcctgcat acagtccgcg tacagtgtct ttttgttctt
     3841 caattgcctt tacaaataca tactgtgttc cttccaaaaa caatacacag tgagcctgta
     3901 tgggcgatag ttcctggtga atttcctttt ggccggtaag tgtaaccaca gtgtcgttat
     3961 caaccacaac cactttgcca ttaatacttc caccccagaa tgtacctgta tgtccgctta
     4021 atcgtttttt ccactctttt ccgttatacg aatacacttc gtttgtatac gttgtgatgc
     4081 aaaactccgg tgaaactcgg tacacttctt caataaacac aatgctgtca gaatagtgtt
     4141 ttcgtggaag gccattgcta atattgataa accttgtgcc tgtgtatcgg tacacaccat
     4201 tccatgatgt ccccacaatg agagtgctac ccttagcata gagtgccgtt atgcatgcat
     4261 tggctggaag gccagtaagg gcaacactct gccagcttgt accaccatcc tctgagcaat
     4321 acagtgaata cttggttgca acgtaaagaa tactggggtc agagccatca acacagaacg
     4381 ctgatatact gcggtaggtg ggaagcaacc cgtttgttgt ccgtatgtga gcatcacggg
     4441 gggtgatatg ttgccaggaa ctatcgccca ggatacgttt aaataagccg tgctcacgtg
     4501 tggaaatata gaatgtattt tttataacct gaatgtcttt tatatgaatt gtatcaggta
     4561 gcccctgatg atatggcttc cccgtataat ataatttccc gtttgtggcc tgatacagaa
     4621 gcggctgcca tgcataggaa agtgatgtaa ctaataaaca aacactaagt gctaaacttt
     4681 tcattgaaca tgctttcatt catgattata taggtatagc cctgctccgt taagaaaagc
     4741 tggcggttat gcgcaaaatt ttcctcaact gtgtgcgccg taatgagcga ataaaaatgc
     4801 gctgtgttat cccccggctt tggacgcagg atgcgcccca accgctgtgc ttcctcctgc
     4861 cgtgaaccaa acgtacccga tacctgaatg gcaacattgg catcaggcag atcaatagaa
     4921 aaatttgcaa cttttgaaac aataagccgt gttatagcac cttgtttaaa ttgtttatac
     4981 agctcctcac gttcttttac gggagtgctt ccggttataa gtggaaatga aaaacgctcc
     5041 tgaatctttt gcagctggtc aatatattgc ccaataataa gaacgcggtc attgggatga
     5101 tgttttaaca attgttccac atagtgaagc tttttttcat tttctgatgc
//
LOCUS       DAOSZP010000087         5113 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_13598_length_5113_cov_3.485567, whole genome shotgun sequence.
ACCESSION   DAOSZP010000087 DAOSZP010000000
VERSION     DAOSZP010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5113
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13598_length_5113_cov_3.485567"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1380)
                     /gene="feoB"
                     /locus_tag="PK348_02920"
     CDS             complement(<1..1380)
                     /gene="feoB"
                     /locus_tag="PK348_02920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HXK65198.1"
                     /translation="MTKKSITIGLVGNPNCGKTTLFNALTGARQKVANWAGVTVERRE
                     GFFHHKGYTIKVVDLPGAYSLTSFSMEELITRNFILLDKPDFIVNVVDAGNLERNLYL
                     TTQLIEIEAPVIIALNMIDEAYDKGLTIKTEKLSELFSMPVVPTVGTKNIGIEQLKNT
                     IVDSYEKQSTHRKIVIPYGNDIEQAIHSLQTIIEKNTVAYPSRWLAISLIEGDSDAEK
                     KISHLPNGDEILKTTVSIRNKLIATFNDDIETVLSDMRYGFISGALKEAVHKKPAKRY
                     DITQKIDAIVLNRYIGFPLLFIMLFILFHLTFILGEYPKQLIEAGVGWISNLFTSIIP
                     DGQLQNLIVQGIIGGVGSVIVFLPNILILFLGISFMEDTGYMARSAFLMDKIMHSLGL
                     HGKSFIPLVMGFGCNVPAIMATRSLENKRDRILTVLINPFMSCSARLPVYILFAGTFF
                     PEHAGIIILF"
     gene            complement(1373..2095)
                     /locus_tag="PK348_02925"
     CDS             complement(1373..2095)
                     /locus_tag="PK348_02925"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HXK65199.1"
                     /translation="MRRRQGNPFNVTDNSSTSIDTPLSPNMEDYMENISMLLKEHDVV
                     RVKDIAKKLNIKMPSVTAALQKLKDKGLVDYEKYGHVQLTAKGKEVADTIYEKHSFLT
                     EFFKDILGMNTQEAEQQACKLEHHLSSDASNRMQRFVEFYKQEQLDSQQWINRLDSVL
                     EEKRLSDLKEGEKAEIVRILGSGPLKKRLLEMGFRKGEIIHVIKYAPLKDPIEISIKD
                     FLLSIRVEEAHSIIVKPLQNHD"
     gene            2796..3380
                     /locus_tag="PK348_02930"
     CDS             2796..3380
                     /locus_tag="PK348_02930"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HXK65200.1"
                     /translation="MDYISTYLSKISKLVVEQRHALGISQEYLAELCKVHRNTIAVLE
                     LGESDPTIVTLTNIFMAFGIQRFIIDHNNCHLQMITGVPFVDLLESSIINDKIGFFID
                     SLRLKHNISRDKLSLATGIHRNTIARIENAQVTARLSTIIQIYRYFNVSEVYTTTNCD
                     PNDCTFYGVTLRCIDISQRNSTMLINSSPVAIRS"
     gene            3445..4170
                     /locus_tag="PK348_02935"
     CDS             3445..4170
                     /locus_tag="PK348_02935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65201.1"
                     /translation="MRKGTQLVVIMAIVTVISFFAVPIAAEAPSGSFIIAHLGYVPIA
                     ATGYDFSAIDSENGWNPNFVVLEHKSQGAALNIEYNFNTNPLLFSFGFEYQLLWDKFS
                     VFQDIGGGVYETIKLKTYLNQYMMPSVTIKYLTASGFYMGTGIALKYLAVTEKVKPDN
                     GYDFSLEFDKKLDFWSITIVGYIISINQGLYLDVQGRFGYNLTNNQFSSLKTNSPGGD
                     MKAYLMPRTMYDAAILVGVGMNM"
     gene            4493..4951
                     /locus_tag="PK348_02940"
     CDS             4493..4951
                     /locus_tag="PK348_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65202.1"
                     /translation="MKRALGVIAVVAIAFIFIAADKTPSADELIDQLGARHSKVFCKY
                     GVPIKMHIDRGPTKEEDYIIFDYLQFGFVFHREMIRQVLFYPEWKGGKIKNINIGMTK
                     DQVIKIMGTPEEEVALNDGRTRITYLEPDKYFDIILSTSNVVTMVRFEVK"
BASE COUNT         1578 a          895 c          975 g         1665 t
ORIGIN      
        1 aaaaagaatt ataataccag catgttcagg gaaaaaagtg ccggcaaaaa gtatgtagac
       61 cggaagtctg gctgagcagc tcataaaagg attaataagt actgtcagta tgcggtctct
      121 tttattttcc agcgaacgag ttgccataat tgcgggcaca ttgcatccaa agcccataac
      181 aagtggaata aaagatttgc catgcaagcc taaactgtgc attattttgt ccatcaggaa
      241 tgcagaacgt gccatgtatc ccgtgtcttc catgaagctg atacccagaa ataaaattaa
      301 tatatttggc aaaaatacga taacactacc aacgccacca ataattccct ggacaattag
      361 attttgcagt tgcccgtctg gtataatact ggtaaaaaga ttgcttatcc accccacacc
      421 tgcttcaatc agctgttttg ggtattcccc caaaatgaat gttaaatgga atagaataaa
      481 aagcatgata aaaaggaggg gaaagccaat gtatctattc agtacaatgg catctatctt
      541 ttgtgttata tcataccgtt ttgcaggctt tttgtggacc gcttctttca aggctccgct
      601 tatgaatcca taacgcatat ctgatagcac tgtttcaata tcatcattaa aggtagcaat
      661 cagcttgttg cgaatagata cagttgtttt cagtatctca tcgccattgg gcaaatgact
      721 gattttcttt tcagcgtcac tgtcaccttc tatcaggctt atagctaacc atcgagatgg
      781 gtacgcaaca gtattttttt cgataattgt ctgtaacgaa tgaattgcct gctctatatc
      841 attaccatag gggattacaa ttttacggtg tgttgattgt ttttcataac tatcgacaat
      901 agtattttta agttgttcaa taccaatatt ttttgtgcca accgttggaa caacaggcat
      961 tgaaaacagt tcactcagct tttcggtttt tatggtaagc cctttatcat acgcttcatc
     1021 aatcatgtta agagcaataa taactggagc ttcaatctca attaattgtg tggtaagata
     1081 tagattgcgt tctaaattgc cagcatcaac aacattgaca atgaaatctg gtttgtccaa
     1141 taaaataaaa tttcgcgtta tcagctcttc catgctgaat gaagtaaggc tgtatgcacc
     1201 aggcagatcc acaaccttga tggtatatcc cttgtgatgg aaaaaacctt cccggcgttc
     1261 cacggtaacg ccagcccagt ttgcaacctt ctgccgcgca ccggtcaggg cattaaacag
     1321 ggtagttttg ccacaattgg gattgccaac aaggccaatg gttatgcttt ttttagtcat
     1381 ggttttgcaa tggttttaca ataatagaat gtgcttcttc gacacgtatt gaaagtaaaa
     1441 aatccttaat tgaaatttca atgggatctt ttaaaggagc atattttata acgtgaataa
     1501 tttcgccttt tctgaatccc atttcaagca agcgcttttt taaagggcca cttccaagaa
     1561 tccttacaat ttcagctttt tcaccttctt taagatcact caagcgcttt tcttcaagta
     1621 cactgtcaag cctgttaatc cattgctggc tatcaagctg ttcctgtttg taaaattcaa
     1681 caaagcgctg catgcggtta ctggcgtcac tgctcagatg atgctctaat ttacaggcct
     1741 gttgttctgc ttcctgtgta ttcatgccaa gaatgtcttt aaaaaactca gtaagaaatg
     1801 aatgcttttc ataaatagtg tcggccactt ctttgccttt agcagtaagc tgcacatgcc
     1861 catatttttc atagtcaaca agccctttgt ctttaagctt ttgtaatgct gcagtgacgc
     1921 tgggcatctt tatattgagc tttttagcaa tatctttaac acggacaaca tcatgctctt
     1981 ttaaaagcat tgagatgttt tccatataat cttccatatt gggcgaaagt ggagtatcaa
     2041 ttgaggtact ggagttatcg gtaacattaa aaggatttcc ctgtcttcgt ctcattctca
     2101 tggcgatatt acatttttct attatagtat aatacaattt actgaatata atataattag
     2161 tcaagactaa tattatgcat tatataaaat ataatttgag cgatagttac tgagtaagag
     2221 tgacatgtca ttttgaataa agcgaagaat cctaaaaaac gtcgatattc ttcgtcctga
     2281 ttttatcggg ataaagaagc attatctttt taagtaatga gattgcttcg tcccgattgc
     2341 atcgggactc gcaatgactt tgcggggaag gtcatcttga gtcccgatcc aattgggacg
     2401 accgaagaat gacccagtag cataaatgcc acccaaagct gaaagaagcg gtcggtcact
     2461 tcgatacaca cgctttgcgt gcactcagtg accgggaatt cgataaatgt gctttgcatg
     2521 cactcggtga ccgggaatcc gatacacgtg gtttgcgtgt actcagtgac cgaaaatccg
     2581 atacacgtgg tttgcgtgta ctcagtgacc gggaattcga taaacgtgct tagaacgcac
     2641 tcggtgatcg tatcgtacta taaatatata taataattta agagaaataa aaaaatacca
     2701 tgtactctga catccttgat taaaatgcat ggttattttt tttaattact tgaaaaattt
     2761 aattgtatgg ttttacaatt gtaaaaaggt gaaaattgga ttatatttct acatatctta
     2821 gtaaaatttc aaagcttgtt gttgaacaac gtcatgcact gggcatatcc caggaatatc
     2881 ttgcagagct ttgtaaggta catcgaaaca caattgctgt attagagtta ggggaaagtg
     2941 atccaacaat agttacattg acaaatattt ttatggcttt tggaattcag cgctttatta
     3001 tcgatcacaa taactgtcac ttacaaatga ttacgggtgt cccttttgtt gatttattgg
     3061 aaagctccat cattaatgac aagatagggt tttttattga ttcacttcga ttaaaacaca
     3121 atatatcccg tgataagctt tcattggcta cgggtattca ccgtaatact attgcgcgaa
     3181 ttgaaaatgc acaggttaca gcacgtctga gtaccattat acagatatat agatatttta
     3241 acgtttccga agtctatact acaaccaatt gcgaccccaa tgattgtaca ttttatggtg
     3301 tcaccttacg ctgtattgac ataagccaaa gaaacagtac catgctgatt aatagcagtc
     3361 ctgtagcaat acgcagttga ttttaaattc tgtacatgca gctacattac ctgctataat
     3421 caaaaaattt atggaggttt tattatgcgt aagggtactc aattagttgt tattatggca
     3481 atagtaactg taatttcatt ctttgcagtt cccattgcag ctgaagcacc atcaggcagt
     3541 tttattatag ctcacctggg ttatgtccca attgcagcaa cagggtacga tttttcagca
     3601 attgatagtg aaaacggatg gaatccgaat ttcgttgtac tggagcacaa atctcagggt
     3661 gctgcattga acatagaata taatttcaat acaaatcccc ttttattttc ttttggtttt
     3721 gaataccagc ttttatggga taagttctct gtttttcagg atataggagg aggagtgtat
     3781 gaaacaatta aattaaaaac atacttgaac cagtatatga tgccaagtgt taccattaaa
     3841 tacctgacag catctggttt ttacatgggt accggtattg cactgaaata tcttgcagta
     3901 acagaaaaag ttaagccgga taatggctat gatttctctc tggagtttga taagaagctt
     3961 gatttctgga gtatcacaat agtggggtat atcataagta ttaatcaggg attatacctg
     4021 gatgtgcagg gacgttttgg gtataactta acaaataatc agttttctag tttaaaaacc
     4081 aatagccctg gtggagatat gaaagcatat ttgatgccaa gaaccatgta cgatgctgcc
     4141 atactggttg gtgttgggat gaatatgtaa agtatgtaac ataggtatat gttcatgaga
     4201 ggttgtttaa aaaattttat tgcttgaata tcataaatcg ttattgtcta tttttcattt
     4261 tcaggtgatt atgctataac ttttgttaaa gttattcaat tcctgtttta ggatttatag
     4321 cataacagcc attttggaca acctcttata catatcatat tgtataaata aaaatattac
     4381 ttcagtaact atcgcccaaa aaaaataaaa ttattacttg ctattaataa tcatttatgg
     4441 cacaataccg aaatatgttg tttattataa taaaatttta aggaggcttc ttatgaagcg
     4501 tgcattaggc gtaattgccg ttgtggcgat agctttcatt tttattgctg cagacaaaac
     4561 accatcagca gatgaattaa ttgatcagct tggtgcgcgt cactcaaagg ttttttgtaa
     4621 gtatggcgtt ccaatcaaaa tgcatattga taggggtcca actaaagaag aagattacat
     4681 catctttgac tacttgcagt ttggttttgt gtttcacaga gaaatgatac ggcaggtcct
     4741 gttttatcct gaatggaaag gtggaaagat taagaatata aatataggta tgactaaaga
     4801 ccaggttatt aaaattatgg gcacgcccga agaagaagtg gcattgaatg atggccggac
     4861 ccgtataacg tatttagagc cggataagta ttttgatatt atcctctcaa caagtaacgt
     4921 tgttacgatg gtgagatttg aagtgaaata acatattaaa gcatacaata aaatataaaa
     4981 tgaaatcaaa ggaagagtat gagttttgaa gtgcctgtaa gtctggttca gtttttattg
     5041 ccaccaattt gctttggaat acttcttggt ggatttattt tatatgtgta tatgtactgg
     5101 agatataaaa att
//
LOCUS       DAOSZP010000088         5110 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_13610_length_5110_cov_3.346785, whole genome shotgun sequence.
ACCESSION   DAOSZP010000088 DAOSZP010000000
VERSION     DAOSZP010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5110
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13610_length_5110_cov_3.346785"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            378..1334
                     /locus_tag="PK348_02945"
     CDS             378..1334
                     /locus_tag="PK348_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011670088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXK65203.1"
                     /translation="MKVKFWGVRGSIPTTLNSVTIRDKIKQALQLATPKDIANDESIE
                     TFLDSLPTSIVGTYGGNTTTLEVRTASDDLIIIDCGTGLKHLGNELLKGDFGNGKGFA
                     TILLTHTHWDHIQGIPFFAPFYIKGNRFNIYSPYNDIKERIEYQQVFTHFPINLEYML
                     AQKEFFIIEKEGEININDVKIKNKRMRHPGGSFGFRFEENGKSFVFTSDCEFNIDEID
                     KIASYKEFFENADVCVFDTQYTFEESINKVDWGHSSASIAIDIAAMFNIKRLILFHHE
                     PDYNDNKLDAVLSNAKLYLGMNPSRRGKLTIDIAREGMEIEI"
     gene            complement(1335..2021)
                     /locus_tag="PK348_02950"
     CDS             complement(1335..2021)
                     /locus_tag="PK348_02950"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HXK65204.1"
                     /translation="MKKYFVIGLGNFGFNIARALEENGCEVLGMDIDRELVQRAKDFI
                     SHAIIGNASDREVLESLAISDFDGAIISIGQDMAASILIALYLKEIGMQKIIVRAISE
                     DHGKILTLIGVDTVVFPERDMAIRLASKLALKNAMDYLPLTEEYGILEVVPPKSFINK
                     SLKELKLSTRFGCQVIGLKYYANGNYNDVTEKNAQIKIAPTADDVVTEHSVMVVIGKL
                     SDIERLQNAD"
     gene            complement(2031..3374)
                     /locus_tag="PK348_02955"
     CDS             complement(2031..3374)
                     /locus_tag="PK348_02955"
                     /inference="COORDINATES: protein motif:HMM:TIGR00933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015379 - potassium:chloride symporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HXK65205.1"
                     /translation="MKKLLEYLSPIEKVLLSFVLLILVGACILCLPVCTTGGISFVDA
                     LFTSTSAVCVTGLIVLDTGKDFTFVGQLVILLLIQLGGLGIMTFSVALLSLMGGSVSI
                     KWRFTLQNMYSDVSKLPIRNILGRIIIYTFTIEITIALILFTQFIKRFTCTQAVWHSI
                     FHSVSAFCNAGFSTFSNNLMDYNGNSVIILSIAIDIIAGGIGFLAMSELFNVKSFRLK
                     QMWKKLTLHTRIVLTTTTILIFGGMLLFYILEYNGILQTLSFKEKLLASFFQSVTART
                     AGFNTVDIGALREGTCLVLIFLMFIGGSPGSIAGGVKTSTFAILWLFLVSRLKGLRQI
                     TVWERALAYDNVERAVTLVVVSGLFIFFATFILVVIPTLPTGEFLQSFFEITSAFGTV
                     GLSMGITPKTVPLERIILCVVMYTGRLGPLTLISALTMRKKTINIKYAEDHIMIG"
     gene            complement(3419..3491)
                     /locus_tag="PK348_02960"
     tRNA            complement(3419..3491)
                     /locus_tag="PK348_02960"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3456..3458),aa:Val,seq:tac)
     gene            complement(3496..3765)
                     /locus_tag="PK348_02965"
     CDS             complement(3496..3765)
                     /locus_tag="PK348_02965"
                     /inference="COORDINATES: protein motif:HMM:NF012442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HXK65206.1"
                     /translation="MTKHEIITRLHLKYGISRDVVQQIVDGFVDEIVVSVQKGEKVTI
                     KGFGVFSRVKQAERKVYSPIAQREVTIPSRYVIDFKSSKLTDIEH"
     gene            complement(3787..4050)
                     /gene="rpsT"
                     /locus_tag="PK348_02970"
     CDS             complement(3787..4050)
                     /gene="rpsT"
                     /locus_tag="PK348_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001274011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HXK65207.1"
                     /translation="MANIKSAEKRNRQSEKRRQRNSQVKSSIRTAEKKVRKQLESQSK
                     ENVPALFKEFVKKIDTAARKGIIHWKTAARKKSRLAKKVNAAS"
     gene            complement(4205..4732)
                     /locus_tag="PK348_02975"
     CDS             complement(4205..4732)
                     /locus_tag="PK348_02975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65208.1"
                     /translation="MKRILSLFTVLSLVIVIAQVWAYVAMDIQKWEIDRKDVVASYIH
                     TSPYKTYTLSQLKGYSNKLLNYILAIDENYKDKVIILRVTGDITRDFMLINNKLYVIT
                     DYMQKSSEDYISGAIAQLEKKYGVPQKQSEGTTTTYTFTRDETKVIAIGENKGNNYSL
                     TIYYYYKNLFRSLMQ"
     gene            complement(4772..>5110)
                     /locus_tag="PK348_02980"
     CDS             complement(4772..>5110)
                     /locus_tag="PK348_02980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65209.1"
                     /translation="SGYCGLRLSPSATGVTGYYIYIMMVIFILRFTVMLLGTSRCAPT
                     ITRHARGCRTAPFVSLTHYYCRKMFDNNSLSGNTLTDLPDVTKRAFVTCNRGIVFIGA
                     CKNVLKPRYG"
BASE COUNT         1680 a         1014 c          870 g         1546 t
ORIGIN      
        1 gggctggccg gtcatcgagc ttgtcgagat gaagcccttt ttcccctttg gaaaagaata
       61 agatggaatt gttgccgtct ggacaaaaaa agtgaataaa aaaagtataa gtaataaaaa
      121 aagatagcgc caattcaagt aaaaagatgg ataaccacaa tagggttaga gcataataat
      181 catttacacg tattcagtga tatctgtgtg agaataaaaa ataataaatc acacctacat
      241 caaagagggg cgacaaaaaa ataggaatgt aaaatcatga aaatggggtc agagccttat
      301 tctaaaatta ttcttgattc tcataaaaac cacgatatta tataatatac ataagataaa
      361 attgaagagg cacctgtatg aaggttaaat tctggggagt acgaggttca atacccacca
      421 cacttaactc tgttactata cgtgataaaa taaaacaggc acttcaatta gcaaccccaa
      481 aagatattgc taatgatgaa tcaattgaaa cttttttaga ttcactgcca acatcaattg
      541 ttggaacata tggtggcaac acaactacac tggaagtacg aacagcttca gatgatttaa
      601 tcattattga ttgcggcacc ggtttaaaac atctaggcaa tgaactctta aagggcgatt
      661 ttggtaatgg aaaaggattt gctactatac tgcttacaca cacacactgg gaccacatac
      721 agggaatacc tttttttgcc ccattttata ttaaagggaa tcgttttaat atctattctc
      781 catacaatga tatcaaagaa cgcattgaat atcagcaggt ttttacacac ttccctatta
      841 atcttgaata tatgcttgct cagaaagaat tctttataat tgaaaaagaa ggtgaaatta
      901 acatcaatga tgtcaaaatt aaaaacaaac gcatgcgaca tcctggtggc tcctttggtt
      961 tcagatttga ggaaaatggc aagtcttttg tatttaccag tgattgtgaa tttaatattg
     1021 atgaaattga taaaattgct tcatataaag aattttttga aaatgctgat gtatgcgttt
     1081 ttgatacaca gtataccttt gaagaatcaa ttaataaagt ggattgggga cattcttcgg
     1141 cttcaattgc aattgatatt gctgcaatgt tcaacatcaa gcgattgata ctgtttcatc
     1201 acgaaccaga ttacaatgat aataaattag atgctgtttt atctaatgca aagctttatt
     1261 tgggcatgaa tccttcacga cgtggaaaat taaccattga catcgcacgt gaaggcatgg
     1321 aaatagaaat ataatcagtc agcattttgc aggcgttcaa tatcgcttaa cttgcctatt
     1381 accaccataa cagaatgctc agttacaaca tcatcagccg tgggtgcaat tttaatctga
     1441 gcattttttt ccgtaacatc attataattc ccgttagcat aatacttcag acctataacc
     1501 tggcatccaa aacgtgtact aagtttcagt tctttaagtg atttgttaat gaaacttttg
     1561 gggggcacaa cttcaaggat tccatattct tcagtaagag gaagataatc cattgcattt
     1621 ttcagtgcca atttgctggc aagacgtatt gccatatcac gttcaggaaa tacaacagta
     1681 tctacaccaa tcaatgttaa aatctttcca tgatcttcgg agatagctct aacaataatc
     1741 ttttgcatgc ctatctcctt taaatacaat gcaataagaa tgcttgctgc catgtcctgc
     1801 ccaatgctga tgatggcacc gtcaaaatcg cttattgcaa gcgattcaag gacttctctg
     1861 tccgaggcat tccctattat agcatggctt ataaaatcct ttgctctttg aacaagctcc
     1921 ctgtcaatat ccatgccaag aacttcacag ccattttctt caagtgctct tgcaatatta
     1981 aatccaaaat tacccaatcc aataacaaaa tattttttca tacacaacct ttacccaatc
     2041 attatatgat cttcagcata tttgatatta atggtttttt tacgcatagt caatgctgaa
     2101 attaacgtca acggacctaa ccttcccgta tacatgacaa cacacaaaat tatcctttca
     2161 agtggaactg tctttggcgt tattcccatt gaaagcccta ccgtaccaaa tgctgatgtt
     2221 atttcaaaaa aagactgtaa aaattccccc gtgggcagcg ttggtatcac aacaagaata
     2281 aatgttgcaa agaaaataaa caatcctgaa actaccacta atgtaactgc tctttctaca
     2341 ttatcatagg ccagtgcgcg ttcccatact gttatctggc gcaatccttt taaccgtgaa
     2401 accagaaaca accataatat tgcaaatgta cttgtcttaa caccaccagc aatagaacct
     2461 ggcgaaccac caataaacat aaggaatatt aataccaggc aggtaccttc acgcaaagct
     2521 ccaatatcca cggtattaaa accagcagta cgtgcagtaa ccgactggaa aaatgatgct
     2581 aacaactttt ccttgaaact taaagtttgc aaaatgccat tatattccag tatataaaaa
     2641 agaagcatcc ctccaaaaat taaaattgta gttgttgtta acactattcg tgtatgcaag
     2701 gttagctttt tccacatctg ttttaatcga aaacttttaa cattaaataa ttcactcatt
     2761 gctaaaaaac caattccacc agcaataata tctattgcta tgcttaaaat aataacactg
     2821 ttgccattat aatccatcag gttattactg aatgtggaaa atccagcatt acagaaagca
     2881 gaaacagagt gaaatatgga atgccatact gcctgggtac atgtaaaacg tttaataaac
     2941 tgcgtaaaca ggataagtgc aatcgttatt tctattgtaa acgtataaat aatgattctt
     3001 ccaaggatat tgcgtatggg gagttttgac acatcactgt acatattctg caacgtgaag
     3061 cgccatttaa tagaaacact tccgcccatg agcgaaagca gtgcaactga aaatgtcata
     3121 attccaagac caccaagctg gataagcaaa agaataacca gctgcccaac aaaggtaaaa
     3181 tcctttcctg tatcaaggac aatcagcccc gtaacacaca ctgccgaagt ggatgtaaat
     3241 aatgcatcaa caaatgaaat gccgccagta gtgcatacag gaaggcacag aatacatgca
     3301 ccaacaagaa ttaatagaac aaagctaaga agaacttttt caattggcga aagatattcc
     3361 agtagctttt tcatagactt ataaaataag ggtatacaag agtataccct tttacctttg
     3421 ggcgctagag gatttgaacc tctgaccctc tgcttgtaag gcagatgctc tcccagctga
     3481 gctaagcgcc ctttgctaat gctcaatatc ggtgagcttg cttgatttaa aatctataac
     3541 atagcgtgaa ggtatggtca cttcccgctg ggctattggc gaatacactt tacgttctgc
     3601 ctgtttaacc cttgaaaaaa caccaaaacc ttttatggtt accttctccc ccttttgaac
     3661 cgataccaca atttcatcca caaagccatc aacaatctgt tgcaccacat cacgggagat
     3721 accatatttt agatgcaacc gggtaattat ttcatgtttg gtcaaaaaat acccctgtct
     3781 gcacaattat gatgcagcat taaccttctt tgccaaacgg gactttttac gtgcagctgt
     3841 cttccagtgt ataattcctt ttcgtgctgc agtatcaatc ttcttgacaa actccttaaa
     3901 cagtgcaggt acattttctt tggactggct ttctaactgc ttacgcactt ttttttcagc
     3961 agttctgata gatgatttta cctgcgaatt tcgctgtctt cgtttttcgc tctgtctgtt
     4021 ccttttttca gctgatttaa tattagccaa tgttacaccc tcgttactct ataatgattg
     4081 ttataatttt cttattgaat ttaatgaatt ggatataaac aaattctatc acaatgtgtc
     4141 aagaatttaa taaaattttt cgtaaaaaaa gaacacaaaa tacactctgc caaacttgct
     4201 gattttattg catcaggctg cgaaataaat ttttataata ataataaatt gtcaaagaat
     4261 aattattacc tttattttcg ccaatggcta ttacttttgt ttcatctcgg gtaaaggtat
     4321 aggttgtagt tgtaccctca ctctgtttct gtggcacacc atattttttt tcaagctggg
     4381 cgatagctcc tgaaatataa tcctctgagg atttttgcat ataatcggta ataacatata
     4441 atttattgtt gataagcata aaatctctgg tgatgtcacc tgtgacccgg agaatgatga
     4501 ccttgtcttt ataattttca tcaatggcaa ggatgtaatt tagcaattta ttggaataac
     4561 cctttaactg gctgagtgtg tacgttttat agggcgatgt atggatataa gatgcaacta
     4621 catccttcct gtctatctcc catttctgta tatccatggc aacatatgcc cacacctgag
     4681 ctatgacaat aacaaggctt aataccgtga ataatgatag tattcgtttc atatatagtt
     4741 gtgctccagt tacttaagac tacataatga tctacccata cctgggtttt agtacgtttt
     4801 tgcaagctcc tataaaaaca attccacgat tgcaagtaac aaatgcacgt tttgtgacat
     4861 ctggtaaatc agttaaagta ttgcctgata aactattatt gtcaaacatt tttctgcaat
     4921 agtaatgcgt gagggatacg aagggtgccg tacggcagcc acgggcgtga cgggtgattg
     4981 taggagcaca tcgtgatgta cccaaaagca tcacggtgaa gcggagtatg aagataacca
     5041 tcatgatgta tatgtaatac cccgtcacgc ccgtggccga aggcgaaagc ctgagcccgc
     5101 agtagcccga
//
LOCUS       DAOSZP010000089         5093 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_13672_length_5093_cov_3.652044, whole genome shotgun sequence.
ACCESSION   DAOSZP010000089 DAOSZP010000000
VERSION     DAOSZP010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5093
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13672_length_5093_cov_3.652044"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1395)
                     /locus_tag="PK348_02985"
     CDS             complement(<1..1395)
                     /locus_tag="PK348_02985"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HXK65210.1"
                     /translation="MDVMRNVLAKYSDASFIMQQRAKTLSYFLTVCIVLVLFFLVAQN
                     LMATRPLFSFINAVLCAILIFLTIATLLLRAGNYSWAANISVIGSVFVLGLLVNFGTM
                     AHNEGIILTNYQFLIFIIFSALFCSRRMVITVAIVSLVFAIISFIRTDLISGKVKTVA
                     IVDFTFELIIITAISYLLLRLMDTTIQKLQEELENRDQLMRIKDLLEAVKDISLKLAV
                     ASHELSDTSKNFSVNAQNQAALAEEITATIEEISAGVENVSNSANYQYDSINSFTRRT
                     EELSSLVSRMEQEINNALQLTASIETHARLGAELLNGMDTSITNIHTSSGEMTNIVKI
                     IKDISDQINLLSLNAAIEAARAGDAGRGFAVVADEISKLADRTSVSVKDIESLISAND
                     KEIQKGMTSVQQTVDTISGIIKGVNEINAMVKVLADYMKQQLEVNVIVTKESGQVKVR
                     SEEIRNAAEEQKNAS"
     gene            1733..2431
                     /locus_tag="PK348_02990"
     CDS             1733..2431
                     /locus_tag="PK348_02990"
                     /inference="COORDINATES: protein motif:HMM:NF016764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HXK65211.1"
                     /translation="MAGSFDFSQFINDSKATVLSPKDYFSSMAKEGGLVEPIIKAVLY
                     GVISGVIYFIFTLLNLSAYTMFGTGAAMALIGPAIFAIIGLFIGGVIMLIISAICGGN
                     TAFEANARVVAALMVLGPVQALFSFLTSISFYLGLIVSALIALYGLYLTFIALVNALN
                     AKENVAKIVIGILAVLYVLSLYGSFKAYRFAGHMGEQMLEQTEKLSSEQQKALEQLQQ
                     LQKSLEKLEQEQNE"
     gene            complement(2474..3088)
                     /locus_tag="PK348_02995"
     CDS             complement(2474..3088)
                     /locus_tag="PK348_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015190871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HXK65212.1"
                     /translation="MIHENVKTDFVSREYTPVIDASAYVHPLAAVIGNVILGKNVMVA
                     PFASVRGDEGQPIYVGDDSNVQDGVIIHALETEHHGHPVEKNLVEVSGKKYAVYVGTR
                     VSLAHQVQIHGPAFVGDSSFIGMQSLVFRARVGKGCVVEPKSLVMGVTIPDGRYVPAG
                     SVIKTQHDADNLPVIDDSYPFKSLNDGVIHVNVALAAGYKAKKI"
     gene            3234..>5093
                     /locus_tag="PK348_03000"
     CDS             3234..>5093
                     /locus_tag="PK348_03000"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXK65213.1"
                     /translation="MFKQFLHKHGISNITLLATESPLHQRIVSYLSRNHITYTVIATD
                     DPLPDTHSLLILPLSMHTITPVIDILQKMLTSTYTPLLLFATDFPITTRFKKRFSIAG
                     IIGILELTERPHFITLETQSLQSNPTASLNALIYTTENLISRNIYKLLSQNILYNALV
                     KLHPSVVCITTLQGTIIYYRAHRQYFTRNNNIKKLLYGKKIQELTTQPEKIEHLFSLI
                     DRNIEQRESEITFRMNNTDTTLLVTGEKIKLYGDLEFYLLIGINITTQRKAEDMLISS
                     ELRYRTLVENLPDGLSLSKGDMLLYANPQFFKLFNFTTETLITDVNLKSLIHPDDWAA
                     LYELLQKPIGNVATINVKAYTATGKPLILELSVLSFMGDSSLYHQIYWHDITQTRQLW
                     DKILQSERLSAIGEMASGITHEFNNILTSIQGYVQYTLNNPDDIEANTKAFKVIEKMT
                     EQGHAILKNLSMLTRKDITNKERHNIADLINDIVRIQEKILIKDNITLIKKFVKNPVC
                     YVDAGLIQQVFLNILLNAVHAIRPKGHGTITITVDESNGNAIIKIHDTGTGIDPKIQD
                     EIFNPFFTTKTGTHNIVGTGMGLSISKNIIEQHNGRIWFHSIPDKETEFIIELP"
BASE COUNT         1598 a         1059 c          849 g         1587 t
ORIGIN      
        1 tgatgcattt ttttgttctt cggcagcatt acgtatttcc tcagacctca ccttgacctg
       61 gcctgactct ttggttacta ttacatttac ctccaattgc tgcttcatat aatcagccag
      121 cacctttacc atggcattta tttcatttac ccctttgata ataccactta tagtatctac
      181 tgtctgctgc acacttgtca tacctttctg tatttctttg tcattagcac taattaatga
      241 ctcaatatcc tttacactga ctgatgtcct gtcagctaac tttgatattt catctgccac
      301 tacggcaaaa cccctgcctg catcgcccgc acgtgctgct tcaatggcag cattcaaaga
      361 cagaagattt atctgatctg agatatcctt gattatcttc actatatttg tcatctcacc
      421 tgaactggta tgtatgttag tgatacttgt atccatgccg ttcagtaatt cagcacccaa
      481 acgagcatga gtttctattg aagcggtcag ctgcagtgca ttgttgattt cctgttccat
      541 ccgtgaaaca agtgacgata gttcctctgt acgccgtgta aatgaattga tgctgtcata
      601 ctgataattt gcactgtttg acacattttc aacaccagcc gaaatttctt caatggttgc
      661 cgttatttct tcagctaaag ctgcctggtt ttgtgcatta actgaaaaat ttttggaagt
      721 gtccgatagc tcatgggaag caactgccag cttgagcgaa atatccttta ccgcttccag
      781 caggtctttg atacgcatca attgatccct gttttcaagc tcttcctgta atttttggat
      841 tgttgtatcc atgagtctta acagcagata gcttatagca gtaattatta tcaattcaaa
      901 agtaaaatca acaatagcaa ccgtttttac cttacctgaa ataaggtcgg tacgtataaa
      961 tgaaataatc gcaaatacaa gcgacacaat agccactgtt ataaccatgc ggcgtgaaca
     1021 aaacaatgcg ctaaagataa taaaaattaa aaactgatag ttggtaagaa tgatcccttc
     1081 attatgtgcc attgtgccaa aatttaccag aagccctaat acaaatactg aaccaatcac
     1141 cgaaatattt gctgcccatg aataattacc tgcccgcaat agcagagtag caatggttaa
     1201 aaaaataagt atggcacata aaactgcatt aataaatgaa aatagtggac gtgttgccat
     1261 tagattttgt gcaacaagaa aaaataatac cagaacaatg catactgtta gaaaataaga
     1321 aagagtttta gcacgctgct gcattataaa cgaagcatcg ctatattttg caagtacatt
     1381 cctcattaca tccatagata ctatctcctg aagtagtttg ttgattttta tgccatgtgt
     1441 aagtttccgc actgcaatac tatatctcgt taaaacaaat gcaattctat ttattctata
     1501 ctttagattt tatcaagtaa aatatagtat gcttacccca ggcacctgcg gcatgagtat
     1561 cttcacttat atattattta aagcaatgat tctttaatta aaaaaactat tgctaaataa
     1621 ttttatgttt gacttatatt tttacatatt gtatttttta gttgtttaca tataactgat
     1681 atgtcaactc cccatgaaaa atttttaatt ttattcaaaa aaggaggaaa gtatggctgg
     1741 tagcttcgat ttttcacaat ttattaatga ttcaaaagca acagtattaa gtcctaagga
     1801 ttatttttca tcaatggcta aagaaggtgg ccttgtcgaa cccattatca aagcagtttt
     1861 gtacggagta atttcaggcg ttatttactt tatttttacg ttacttaacc tttctgccta
     1921 taccatgttt ggcacaggtg ctgccatggc tttaattgga cctgccatct tcgcaattat
     1981 tgggcttttt attggtggcg tcattatgct cattatttca gcaatatgcg gcggcaatac
     2041 tgcctttgaa gcaaatgccc gtgttgtggc tgcattaatg gttcttggtc ctgttcaggc
     2101 attattttca tttttaacaa gcatcagttt ttatttgggg cttatagtat cagcgttaat
     2161 tgccctgtat ggcctgtacc ttacctttat tgcacttgtg aacgcgttaa atgctaaaga
     2221 aaatgttgca aagatagtca taggcattct tgctgtattg tatgttcttt cactctatgg
     2281 ttcatttaaa gcatacagat ttgcaggaca catgggtgaa cagatgttag aacagaccga
     2341 aaagctctct tcagaacaac agaaggcttt agaacagtta cagcaattac aaaagtcatt
     2401 agaaaagctt gaacaggaac agaatgaata atcttacaaa agaccccctc ttaaaaagag
     2461 ggggtcttgg tatttatatc ttttttgcct tatatccagc agcaagcgct acgtttacat
     2521 gaatcacgcc gtcattcaaa ctcttaaaag gatagctatc atcaataacg ggaagattat
     2581 cagcatcgtg ttgagtttta ataactgacc ctgcaggtac atatctgcca tcaggtatgg
     2641 tcactcccat aaccagcgat tttggttcta ccacacagcc tttgcctact cgtgccctga
     2701 atacaagact ttgcattcct ataaaggaac tatcgcccac aaaagccgga ccgtgaatct
     2761 gcacctgatg agcaagtgac acacgagtac caacatatac ggcatatttt ttaccactta
     2821 cttccaccag atttttttct accggatgac catggtgttc tgtttcaagg gcatgaatga
     2881 taacaccatc ctggacattt gaatcatcgc caacataaat gggctgccct tcatcccccc
     2941 gcacagaagc aaacggtgct accataacat ttttcccaag tatcacattc ccaataaccg
     3001 cagcaagagg atgcacatag gctgatgcat cgatgaccgg tgtgtattcc ctgcttacaa
     3061 aatcggtttt tacattttca tgaatcatag atcctccaaa attgcatttt taattatccc
     3121 ttatgtacca ttacaatctg tcaacataat ttatattgtt ttttaatgta agaaaaaagc
     3181 gttgatgaat atgcatataa ttatttaatt atactctgta tacaggatac ggtatgttta
     3241 aacaattttt acataagcat ggcatcagca atataacgct gcttgccact gaatcgccgt
     3301 tgcatcagcg gatcgtgagc tatctttcac gcaatcatat aacatatact gtaattgcca
     3361 ctgatgaccc tttgcctgat acacattccc ttttaatact acccttgagc atgcatacca
     3421 ttacaccagt aattgatatc ttacaaaaaa tgcttacatc tacatatacc ccgcttttac
     3481 tgtttgctac cgattttccc ataactaccc gatttaaaaa acgttttagt attgcaggaa
     3541 ttatcggcat acttgaactg acggaacgcc ctcacttcat cacgttagaa actcagtcac
     3601 tgcagtcaaa tcccacagct tcactgaatg cgctaatcta tacaactgaa aatctgattt
     3661 cacgcaatat atataaattg ctatcgcaaa acattttata taatgctctg gttaaactgc
     3721 atccatcggt agtatgcatc accacattac agggaacaat aatatattac agagctcacc
     3781 gacaatattt tacccgaaac aataatatta aaaaattatt gtacgggaaa aaaattcagg
     3841 aattaaccac acaaccggaa aagatagaac atctgttttc attaattgac cgcaatattg
     3901 aacagcgtga atcagaaata actttcagaa tgaataatac tgacaccaca ctgcttgtga
     3961 cgggcgaaaa aattaagctg tatggtgatc tggaatttta cctgcttatt ggtataaata
     4021 tcaccactca acgtaaagct gaggacatgc tcatttcgtc tgagctaagg tatcgtacgc
     4081 tggttgaaaa tcttcctgat gggctgtcac tttcaaaagg ggatatgctt ttatatgcta
     4141 acccacaatt ttttaaatta ttcaatttca ccacggaaac tctgattacg gatgttaacc
     4201 ttaaatcact catccatccc gatgactggg cagcacttta tgaactccta caaaaaccca
     4261 ttggcaatgt agcaactata aatgtcaaag cctataccgc taccggtaaa ccgctgatac
     4321 ttgagcttag tgtcttaagt tttatgggcg atagttcctt atatcaccag atttactggc
     4381 acgatataac gcaaacacgc cagctgtggg ataagatact gcagtctgaa cgattgtctg
     4441 ctatagggga aatggcttca ggtattaccc acgaattcaa caatattctc acaagcattc
     4501 agggctacgt ccagtacact ctgaataatc ctgatgatat tgaagccaat acaaaggcct
     4561 ttaaggttat tgaaaagatg accgaacagg gccatgccat ccttaaaaat ctttccatgc
     4621 ttactcgtaa ggacattact aataaagaac gccataacat agcggatctc attaatgata
     4681 ttgtacgtat acaggaaaaa atattaataa aggacaacat cactctgata aaaaaatttg
     4741 taaaaaatcc agtgtgttat gtggatgccg gccttataca gcaagtattt ttaaacatac
     4801 tgcttaatgc agtacatgct atacggccaa aaggtcatgg aactatcacc attactgttg
     4861 atgaatcaaa tggcaatgca attattaaaa tccatgatac cggtaccgga attgacccaa
     4921 aaattcagga tgaaattttt aatccttttt ttacaaccaa aactggaaca cataacatcg
     4981 ttggcacagg aatgggttta tccatttcca aaaatatcat tgaacagcat aacgggagga
     5041 tctggtttca tagcatccct gacaaagaaa ctgaatttat tatagaactt cct
//
LOCUS       DAOSZP010000090         5060 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_13792_length_5060_cov_3.142657, whole genome shotgun sequence.
ACCESSION   DAOSZP010000090 DAOSZP010000000
VERSION     DAOSZP010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5060
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13792_length_5060_cov_3.142657"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="PK348_03005"
     CDS             complement(<1..226)
                     /locus_tag="PK348_03005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65214.1"
                     /translation="MRSNQEFYTYLQYSLYEAIAFFNFRIYIFGSEKTIKAIMFDSYC
                     EKEVLKSIINSAHKGLTPVIKEAIHFFKQYE"
     gene            complement(229..1161)
                     /locus_tag="PK348_03010"
     CDS             complement(229..1161)
                     /locus_tag="PK348_03010"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004162.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HXK65215.1"
                     /translation="MTVLYNLSERDEVFKNPVITIGSFDGVHRGHRAIFDRMLELAQS
                     LHGEPVVFTFSEHPRKLLTPHTPPKILTTAAEKIHAIEQVGINNIVMMPFTHEIAALT
                     AEEFLFKIVFYHIGKAHIVVGYDHAFGKGRQGDFEFLKGLSLREGFLVERVEPVCIQS
                     RPVSSSWIRTEIEDGNIALANMLLGRPYTLRGTVVKGQMRGRLLGYPTANITPDDPDK
                     IIPKDGVYAVRVHINGMLYHGMLNIGTNPTFENTERTIEVHIFDFDADIYGQTLEVFF
                     IERIRDEKRFGSVTELKEQLHNDKLASEKILAGN"
     gene            complement(1145..2218)
                     /gene="aroC"
                     /locus_tag="PK348_03015"
     CDS             complement(1145..2218)
                     /gene="aroC"
                     /locus_tag="PK348_03015"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HXK65216.1"
                     /translation="MHGTNTFGQVLKVTTFGESHGKALGAVVDGLKPGIPLSVEDIQK
                     QLDRRKPGQSSVSTQRKEADSVEIVSGLFEGKTTGTPLCMIVYNKDQDSRAYESIKDI
                     FRPGHADFTFWQKFGIRDYRGGGRSSGRETVGRVAAGAIALKMLKEKGVTIVAYAEEI
                     AGIKGEKVDIDFIEKNPVRSADPDKAQAMEQAIKQAQAEHDSVGGIVACIIKGLPAGL
                     GDPVFCKLDARLAMAVMSIGAVKGIEFGSGFAAARKRGSENNDQMSDGKFLTNNAGGI
                     LGGISTGQDIVMRIAVKPTPSIAKPQKTMDIDGNTVDISIKGRHDPCIVPRIIPVVEA
                     MVALVVYDAWLLQERIGRYDGTV"
     gene            complement(2267..2752)
                     /locus_tag="PK348_03020"
                     /pseudo
     CDS             complement(2267..2752)
                     /locus_tag="PK348_03020"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
     gene            3314..4063
                     /locus_tag="PK348_03025"
     CDS             3314..4063
                     /locus_tag="PK348_03025"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HXK65217.1"
                     /translation="MAARNKGIQLMINKIEKAPDIPSMVVSQIIELISSGVLKPGDRL
                     PSELEMTRRFGISRISLREAMKLLEAKGYIESQGRKGKYVKSVVDDAIKTPIEGLIQI
                     DHKKIWELLEVRRIIDAEAAALAALRANEEQRRKLNSFRTQIELMGVENLIQKREGGK
                     FYAQFYNHLYEATNNTIFAHILNAISTILRGVLPYSREKLAKVKNTSKDIYEHHIKIL
                     DAINSHKPDMAREAVVEHIDYLERTLKKILK"
     gene            4282..>5060
                     /locus_tag="PK348_03030"
     CDS             4282..>5060
                     /locus_tag="PK348_03030"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXK65218.1"
                     /translation="MELTFNLPGLETIEQRTVPLDSSTLYDVLIVGGGPAALTAAVYC
                     MRKGVKTGLITLDVGGQVAETSTVENYMGYRHIEGISLVQRFKEQVEQFEIGFLQGYK
                     AKKVIDGNIKKVECEDGNIYQAKSIIITTGKRWRRLNVPGESELTGKGVAYCAICDAP
                     LFKDKDVIVVGGGNSAIEAAIDLAKLATSVTVVQLLPALTADKVLIDELSKFTNVTIL
                     YEHEIKAIHGTSAVEAVTVHDIKNKKENTLSVQGIFIEIGLV"
BASE COUNT         1605 a         1050 c          827 g         1578 t
ORIGIN      
        1 gttcatactg cttaaaaaaa tgtattgctt ctttgattac gggagttaaa cctttgtgag
       61 ctgaattgat tatgcttttc aatacctctt tttcacagta actatcgaac ataattgctt
      121 taattgtttt ttcactacca aaaatataaa tcctgaaatt aaaaaatgca attgcttcat
      181 acagtgaata ttgtaaatat gtatagaatt cctgattact gcgcatggtc agttcccagc
      241 taatattttt tcactggcca gtttatcatt atgcagttgc tccttaagtt cagtaacaga
      301 accgaaccgc ttttcgtcgc gaatgcgttc aataaaaaac acttcgagag tttggccata
      361 tatatctgca tcaaaatcaa atatatgaac ttcaattgtt cgttcagtgt tttcaaatgt
      421 tggattggta ccaatattaa gcataccatg atacagcata ccatttatgt gtaccctgac
      481 tgcatacact ccatcctttg gaattatctt atcagggtca tcgggggtaa tgttggctgt
      541 agggtacccc aaaagccggc cacgcatttg ccctttaacc acagtaccac gcaaggtata
      601 gggccttccc aaaagcatat ttgccagtgc gatatttcca tcctcaatct cagtgcgtat
      661 ccatgatgaa gaaaccggac gcgactgaat acaaacaggc tcaacacgct caaccaaaaa
      721 cccttcacgt aatgacaaac cctttaaaaa ttcaaaatct ccctgccttc ctttcccaaa
      781 cgcatggtca tatcctacca caatatgtgc tttccctata tggtaaaata caatcttaaa
      841 taaaaattcc tctgctgtaa gggctgcaat ctcatgggta aagggcatca tgacaatatt
      901 gttaatgcca acctgttcaa ttgcatggat tttttcagca gcagtcgtca atatcttagg
      961 tggtgtgtgt ggtgttaaaa gttttcgcgg atgttcactg aatgtaaaaa ctaccggttc
     1021 cccatgtaat gattgtgcta attccagcat acgatcaaat attgccctgt gtcccctatg
     1081 gactccatca aagcttccta tggttattac aggattttta aaaacttcat cacgctcaga
     1141 taagttatac agtaccgtca taacgcccta tcctctcctg aagcaaccac gcatcatata
     1201 ccaccagagc aaccattgcc tcaacaactg gaataattcg tggcacaata cagggatcat
     1261 gccttccttt aattgaaata tcaacggtat ttccgtctat atccatagtt ttttgtggct
     1321 ttgctataga gggggtcggc ttaactgcaa tacgcatcac aatatcttgc cctgttgaaa
     1381 tacctcctag aataccacct gcattattag tcaaaaattt tccatcactc atctggtcat
     1441 tattttcact tcccctctta cgtgcagcgg caaacccgct tccaaactca atacccttta
     1501 ctgcaccaat tgacataacg gccatcgcaa gccgcgcatc cagcttacaa aataccggat
     1561 cgcccaaacc tgcaggcaat cctttaataa tacatgctac tataccgcca acagaatcat
     1621 gttcagcctg agcctgttta attgcctgtt ccattgcctg agctttatcc ggatcggcag
     1681 agcgcacagg atttttttct ataaaatcaa tatctacttt ttctcctttt atgccagcaa
     1741 tttcctcagc atacgccact attgttacac ctttttcttt caacattttc agggcgatag
     1801 ctcccgcagc aactctaccc actgtctcac gcccggatga ccttcctcct cctcgataat
     1861 cacgtatacc gaatttctgc cagaatgtaa aatctgcatg acctggcctg aatatatctt
     1921 taatagattc ataagccctg gaatcctgat ctttattata cacaatcata cacagcggtg
     1981 tccctgttgt tttaccttca aacaaaccag aaacaatttc cacactgtca gcttctttcc
     2041 gctgggtgct aacagaactt tgacctggtt tacgccgatc aagctgtttc tgaatgtctt
     2101 caacagataa tgggatcccg ggtttcaacc cgtcgacaac agcccccaat gctttcccat
     2161 gactttcacc aaatgttgtt accttcagaa cctgtccaaa ggtattggtt ccatgcatac
     2221 attgtgataa tacaaaagat atctggtata gtaaatcctc atgagcagta tcttcatttt
     2281 cttttgttac agtataaata atatcactta tatgttccgt ggcttcatat attttttgaa
     2341 cacgttggct atacttatca aatgcattgt ctccctgtaa aaatggcggg attccggtaa
     2401 cctgcatcct ttgccataaa aaatcaactg tagctttcaa taaaatcatt acactttctt
     2461 ttctaagctg tattctgttt tccgggaata tcattgctcc gccaccagta gctattatac
     2521 accagtcata caacgcagct tttttaattg cctctttttc aagctctctg aaaaatgatt
     2581 caccatgatc agttgcaatc tgcctgcaac tttttttatt gccatgtttt tcttcaaaaa
     2641 gagtttcaat catctcatca gtttcaaaga aaggtatatg taatatttca gctattctct
     2701 tcccaattgt tgattttccc accccttttg ggccagtaat aattatcttc atggtttatc
     2761 agcaccctcc acataccggt caaattaata tcttttttca ctacatggta tttaaaaata
     2821 ctcgtcaatt aaaacttttt ataccattgt agtttactgt acataccata gcatgtagct
     2881 ttcgtttata cgcaacatat acatttactg taatgcaaat tactatacac aactatcaaa
     2941 gtaacagagg ggtatatact ttttaattat accattgttt taatcagtaa ttcatataaa
     3001 taattatcta cccttacaat accacctgta ttatagcaat attacaaaaa ttcaaaacat
     3061 tgacttacaa taaatcaaat catatttttt ttagattgtt tgtatagtaa tgatataata
     3121 atacaataat tttccagttc ttttaattat attattttgt atatattcca ataccaattt
     3181 gcaatattaa tgttgttgac ataattaatt taatattata ttatgtatta cgatattact
     3241 aaatacaatc agattaaata gttattctca ataattaaaa acaacacatc aataaaaaaa
     3301 ggagatctaa tacatggcag cacgaaataa aggcatacag ttaatgatca ataagattga
     3361 aaaagctcct gatatcccaa gcatggtggt ttcacaaatt atagaactca tttcttccgg
     3421 tgttctaaaa ccaggtgaca ggcttccatc tgagctggaa atgacacggc gttttggtat
     3481 cagccgtatt tcattgcgag aagcaatgaa gcttttagaa gctaagggat atattgagtc
     3541 tcagggccga aaaggtaaat atgtaaagtc agttgttgat gatgcaataa aaactccaat
     3601 cgaaggttta attcagattg accataaaaa aatatgggag cttttggaag ttcgccgcat
     3661 tattgacgct gaagccgctg cattagcagc cttacgtgca aatgaggagc aaagaagaaa
     3721 acttaactca ttcagaactc agattgaatt gatgggagta gaaaacctga ttcaaaagcg
     3781 cgaaggtggt aaattttacg cacaatttta taatcacctt tacgaagcaa ccaacaatac
     3841 catttttgcg cacatactca atgcaatatc aactatcctg aggggtgtcc ttccttatag
     3901 ccgtgaaaag ttggccaaag taaaaaacac atcaaaagat atttatgaac accatatcaa
     3961 gattctggat gccataaact cccataaacc agatatggca cgtgaagctg ttgttgaaca
     4021 catagactat ttagagcgca cattgaaaaa aatattaaag taaaatatct accacgataa
     4081 atgggcacac gttaatgtgc ccattttcat tcatttttta tataagctgc ttttactttg
     4141 aagcactcaa taattatatc atattttcaa actccattga acaattcata ttataagaga
     4201 atagaatact taatttattc cttgacgatt aataaatttt ttattactat ttcaagataa
     4261 gaatagttaa aaggagtatc catggaatta acatttaacc ttccgggact tgaaacaatt
     4321 gagcagcgaa ctgttccgct ggatagttca acactatatg acgtgttgat agttggtggt
     4381 ggaccagcag cattgactgc tgcagtctat tgtatgcgca aaggagttaa aactggtctt
     4441 attacactgg atgttggtgg ccaggttgcc gaaacatcaa cagtagaaaa ttatatgggc
     4501 tataggcaca ttgaaggaat ctcactggta cagcgtttca aagaacaggt ggaacaattt
     4561 gagataggat tccttcaagg atacaaggca aaaaaagtta ttgatggaaa tattaaaaaa
     4621 gtagaatgtg aagatgggaa tatctatcag gcaaaatcaa ttattataac taccggtaag
     4681 cgatggcgaa gactcaatgt cccaggtgaa tctgaactta ccggtaaagg tgttgcatac
     4741 tgtgctatct gcgatgctcc actcttcaag gacaaagatg ttattgttgt tggtggtgga
     4801 aactcagcca ttgaagctgc aatagatctg gcaaaacttg caacatcagt aacagtggtt
     4861 caactgttac ctgcattaac tgctgataaa gtgcttattg atgaactatc aaaattcact
     4921 aatgtcacca tattgtatga acatgaaata aaagcaatac acggaaccag tgcagtagaa
     4981 gctgttaccg ttcatgatat taaaaacaaa aaagaaaata cattgtcagt gcaggggatc
     5041 ttcattgaga taggtttggt
//
LOCUS       DAOSZP010000091         5042 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_13850_length_5042_cov_3.484259, whole genome shotgun sequence.
ACCESSION   DAOSZP010000091 DAOSZP010000000
VERSION     DAOSZP010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5042
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13850_length_5042_cov_3.484259"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2026)
                     /locus_tag="PK348_03035"
     CDS             complement(<1..2026)
                     /locus_tag="PK348_03035"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HXK65219.1"
                     /translation="MITLSLIGSLLSSFMLLFTIFYGLYKNYHDRILRYYSYFGLCAF
                     GILFTMFLTYAFPNELDLTLVNKITQASTVLTFSALFVLSLIFPKTEKQVPFLVITII
                     LVPAYIIAYIAVFTDMNITAAYFKDGKLVREFRFFYTVYLSVAFGYVLLGVINFIRKY
                     ITTKVELYRKQMRFVFIGTGIAMTFAAVFSIIMPRFFGYVDLYVLGPSLATFIGVSSL
                     FYAVISYQMMDIRTAIHKTTMYTVISTAIFLPMFALVYIYDINILGLKTIPLYIIAFI
                     IVVMFMVLSKYIQPIIDKAFKRKHYELQEYLDTFVKEISTIRDIDELIKKTVSTIHNT
                     FYLKSTYFLLYNNEVRSYQLEYSMGEAPVSPSINRLSPIVRWFNRNQRIVTLDNVYID
                     ETSFIDVRDEFIQYFTTNNIIIMIPIYHRRVLTGILCLGEKDTLAAYKPDEIELLSFL
                     SNETNINLSHAITYQEAKKEQMLQRTITISSELLAKAVPRALPNIAGIKYGAFYIPRQ
                     GGGIDYFDFIRPGLQGIGTIATDIAGIGMQSALYSVIMRSAFHTSLSDAHSSYSVIQK
                     LNRVIHNYSQGKGILITAYYYYIDVRNMRLIYTNAGFPPMELYRVEKSDFSSLDTEGI
                     PLGYDPTFNYGMGRTNILRGDIGILYSKALITSKNSRGEQFTLDHVRNIIK"
     gene            2298..2825
                     /locus_tag="PK348_03040"
     CDS             2298..2825
                     /locus_tag="PK348_03040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65220.1"
                     /translation="MLKQRKHYLIDRSIQVTIATKILVIPIILLTCFSLILLFFVYQN
                     SNYISKISANQENLIEMFMTTPALMDKENQIVVAGEKTFRENLGILQSIKNNNLILLY
                     CAIGITIFQAVLLFVYGVYLTHRIAGPVYVMRQYLKEIHQGKTPQFRQLRKGDLLTDF
                     YDDFVKAIKTLQTKK"
     gene            complement(2837..3484)
                     /locus_tag="PK348_03045"
     CDS             complement(2837..3484)
                     /locus_tag="PK348_03045"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HXK65221.1"
                     /translation="MNHIPVHKLSEYIDGILDAQDNDAITSHVSECPECRQNLLQLQK
                     MIALLSSLKTISIPATFATDTLARAKKQYYHKRNYRSLRGSVFLAAMMLIAIITMLPD
                     SIKHDQFKNHITQNTQNNQRLDCIIPTNMDLNTAMMVIQQHNARVLDFSNSYVIVESD
                     VSSFSSIRQIINNYESYRPNPLSNVGITYMQQQEKTYPELTHANKKKYIFRLQLR"
     gene            complement(3481..4044)
                     /locus_tag="PK348_03050"
     CDS             complement(3481..4044)
                     /locus_tag="PK348_03050"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HXK65222.1"
                     /translation="MSEYSDNELIDRVIAGDTDAFADIITRYQNKIFRYVYTRVYDYD
                     EACDMTQDIFLITMESLKTFRKESQFSTWLFSIAVNYCKNHRRKNRHKVYSIHSAQDE
                     TEIQIPDIRQNQEELVITNESLQIMLEEIHKLPDDYRDILVLRDIEGESYSAIAQALH
                     LSLANVKVRIHRGREMLKTRLQQRGLL"
     gene            complement(4096..>5042)
                     /gene="tyrS"
                     /locus_tag="PK348_03055"
     CDS             complement(4096..>5042)
                     /gene="tyrS"
                     /locus_tag="PK348_03055"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013537758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HXK65223.1"
                     /translation="RKRLTREEVLSNAETYKKQVFKILDPEKTIVDFNSRWCMPMSFA
                     DVLDLASRYNVARMLERDDFAKRYKDGVPISILEFLYPLIQGYDSVALKADIELGGTD
                     QKFNLLVGRDLQREYGQEPQVIMTLPLLVGLDGVQKMSKSLNNYIGIDEDPQQIFGKV
                     MSISDDLMFLYYELVTDVPKAEIESYKKGIQDGSVHPKDVKVRLAKEICAQFYNEEIA
                     QKAKMEFDKIFVQKDLPSEMPEFKVPESEKKNNTIWIVKLLVLAGMAKTNGEARRLIE
                     GGGVYIDGNKVENPDAEIPCSASFIIKVGKRRFARILP"
BASE COUNT         1617 a          886 c          908 g         1631 t
ORIGIN      
        1 cttttattat atttcgtaca tgatctaatg taaattgttc accacgagaa ttcttagatg
       61 taatcaatgc tttggaatac agaatgccaa tatctcccct gagtatgttg gttcggccca
      121 tgccataatt gaaggttgga tcatatccca gtgggatccc ttcagtatcc agtgatgaaa
      181 agtcgctttt ttctacacgg tataattcca ttggtggaaa tccagcattt gtgtaaataa
      241 gcctcatatt gcgaacatca atataatagt aataggctgt aattaatata cctttaccct
      301 gagaatagtt atgaataacg cggttaagct tttgtatgac actatatgat gaatgagcat
      361 cactgaggct ggtgtggaaa gcagacctca tgataacaga atataatgca ctctgcatac
      421 caatgccggc aatgtcggtg gcgatagtac ctattccctg taaacccggg cgaatgaaat
      481 caaagtaatc aatacctcca ccctgccgtg gaatataaaa agcaccatat tttatgcctg
      541 caatatttgg caatgctctg ggaacagctt ttgctaaaag ttcgcttgaa atggtaattg
      601 tgcgctgcaa catctgttct ttttttgctt cctgatatgt tattgcgtga gaaaggttaa
      661 tattggtttc attggataag aaagataata gttcaatctc atcaggttta tatgctgcca
      721 gtgtatcctt ttcacccaag catagaatac cagtcaaaac acgacgatga taaataggaa
      781 tcatgattat aatattattg gttgtaaaat attgaataaa ttcatcccgt acatcaataa
      841 atgaagtttc atctatgtaa acgttatcca gagttactat cctctggttt ctgttgaacc
      901 agcgtactat aggtgataag cggtttatag aaggtgaaac aggtgcttct cccattgaat
      961 attcaagctg atagctccgg acttcattat tgtacagaag gaaatatgtt gattttagat
     1021 aaaatgtatt gtgtatagta gaaactgttt tttttataag ttcatcaatg tcccgtattg
     1081 tacttatttc tttgacaaat gtatccagat attcctgcag ttcataatgc ttgcgtttaa
     1141 aagctttatc tattataggt tgaatatatt ttgaaagaac cataaacatg acgactatga
     1201 taaatgctat aatatagaga ggtatggttt ttaatcccag tatattaata tcataaatat
     1261 aaaccagtgc aaacatgggt aaaaatatgg ctgtggaaat gactgtatac atggttgttt
     1321 tatgaatagc ggtcctgata tccatcatct ggtatgaaat aactgcatag aaaagtgatg
     1381 atactccaat aaatgttgcc agtgatggtc ctaaaacata gagatcaaca taaccaaaga
     1441 atcgtggcat aatgatggaa aaaacagcag caaacgtcat tgctatacca gtaccaataa
     1501 acacaaagcg catctgcttg cgatacagtt caacttttgt ggttatatat ttacggataa
     1561 aattaataac acctaaaaga acatatccaa acgctactga taaatataca gtataaaaaa
     1621 atctgaattc acgaactaat tttccgtctt taaaatatgc tgcagtaata ttcatatcag
     1681 taaaaactgc tatgtaggca atgatgtatg caggaacaag aataatagta ataaccagaa
     1741 atgggacctg tttttcggtt tttgggaata ttaaggatag aacaaataaa gctgaaaaag
     1801 ttaataccgt tgatgcctga gtaattttat ttactaatgt tagatcaagt tcattgggga
     1861 atgcatacgt taaaaacatg gtaaaaagta tgccaaatgc acacaatcca aagtatgagt
     1921 aataccgtaa tattctatca tgatagtttt tatatagtcc ataaaatata gtaaataaca
     1981 gcataaatga tgacaataat gaaccaatta aactcaaggt aatcatgggg atattcctcc
     2041 caattaaagg tttgtaatgg gatacgtacc actttaaact tgctcttacc gatgttgtaa
     2101 gaacaattag aacaaaatag tacattcgtt gctaataata gctttttatt tacctgattg
     2161 tcaagtttta ttagtaaaac taaaaacgta gttgacgtaa tataatttat actatataat
     2221 tacagaaaaa taattaagat gctacatatg atttttacac gggtatataa aaaataatta
     2281 atgtagagga ataagttatg ttgaagcaac gcaaacatta cctcatcgac aggtccatcc
     2341 aggttactat cgcaacaaaa atactggtta taccaattat actgttgacc tgtttttcgc
     2401 tcattctatt attttttgtg tatcagaaca gcaattatat tagcaagatt tcagctaatc
     2461 aggaaaacct catagaaatg tttatgacaa cacccgccct tatggataag gaaaatcaga
     2521 tagtagtggc tggtgaaaaa acattcagag aaaatttagg catactacaa tcaattaaaa
     2581 acaataatct tattctttta tattgtgcga ttgggataac aatatttcag gcagtgttgc
     2641 tttttgttta tggtgtgtat cttacccata gaattgcagg tcctgtgtat gtcatgcgcc
     2701 agtatttaaa agaaatacat cagggcaaaa ccccacaatt cagacaatta cgcaaaggcg
     2761 atcttttaac tgatttttat gatgactttg taaaggctat taagacatta caaaccaaaa
     2821 agtagaaaat atatttttag cgaagctgca aacgaaaaat atacttcttt ttgttggcat
     2881 gtgttaattc cgggtaagtt ttttcctgtt gttgcatata ggtaattccc acatttgata
     2941 acggattggg gcgatagctc tcataattgt ttatgatttg ccggatagat gaaaaactgc
     3001 tcacatcaga ttccacaatg acatacgagt ttgaaaaatc aagtacacgg gcattatgct
     3061 gctgtataac catcattgcg gtgttcaggt ccatattggt tggaatgata caatctaacc
     3121 tttgattgtt ttgagtattc tgtgtaatgt gatttttaaa ttgatcatgt ttgattgagt
     3181 cgggcagcat ggttattata gcaataagca tcatagcggc tagaaaaact gaaccacgca
     3241 gtgaacggta attccgttta tgataatact gttttttagc ccgtgccaga gtatcagttg
     3301 caaatgttgc aggtatagat attgttttca gggaggatag tagagcaatc atcttctgaa
     3361 gttgaaggag gttttgcctg cattcaggac attctgatac atgtgatgta atagcatcgt
     3421 tatcctgggc atcaagaatg ccgtcgatat attcagaaag cttatgaaca ggtatatgat
     3481 tcatagaagc cccctttgct gcaaacgagt ttttaacatc tcgcgccctc ggtgtatgcg
     3541 tactttaaca ttggccaaac ttaaatgtaa cgcttgtgct atagctgaat acgattcacc
     3601 ttctatatct cgtaaaacaa gaatgtccct gtaatcatcc ggcaatttat ggatttcctc
     3661 aagcatgatc tgtaatgact cattggttat gacaagttct tcctggttct ggcgaatatc
     3721 gggaatctgt atttctgttt catcctgggc agagtgtatt gaatatactt tatgtctgtt
     3781 ctttcttctg tgatttttac agtaattaac agcgatgcta aaaagccatg tagaaaattg
     3841 agattctttt cggaatgttt ttaacgattc catggtaatg aggaatatat cctgggtcat
     3901 atcacatgcc tcatcataat catacacacg tgtatacaca tacctgaaaa tcttattctg
     3961 gtatcgtgta atgatatcag caaatgcatc agtgtctcct gcaattaccc tatctataag
     4021 ttcattatca ctatattctg acatagttag tagatataag ttactgaaaa aattagtgta
     4081 gaaacgggaa aaaagttacg gaagtattct ggcaaaacgg cgttttccaa ctttaataat
     4141 aaaagatgca ctgcagggaa tctcggcatc agggttttct accttgttgc catcaatata
     4201 cacaccacca ccttcaataa gcctgcgtgc ttcaccattt gtttttgcca tgccagccaa
     4261 aaccagtaac ttcacaatcc atattgtatt gttctttttc tcagattcgg gtactttaaa
     4321 ttctggcatc tctgatggca ggtctttctg tacaaagatt ttgtcaaatt ccatctttgc
     4381 tttttgggct atctcttcat tataaaactg tgcacatatt tccttggcca gccttacctt
     4441 tacgtctttt ggatgcactg atccatcctg tatgcctttt ttgtagcttt caatttcagc
     4501 ttttggaaca tcagtgacga gttcataata taaaaacatg agatcgtcag atattgacat
     4561 aacctttcca aaaatctgct gtgggtcttc atctattcca atataattat taagcgattt
     4621 actcattttc tggacaccat caaggcctac cagtaacggt agcgtcatga taacctgtgg
     4681 ctcctgtccg tattcacgct gcaggtctct tccaaccagc aggttaaatt tctgatcggt
     4741 accgcctaat tcaatatcag ctttcagtgc cacggaatcg tatccctgaa ttaagggata
     4801 taaaaattcc agtatggaaa taggtacacc atctttatag cgtttggcaa aatcatcacg
     4861 ctccaacatt cgtgcaacat tatagcgtga agccaggtct aaaacatcgg caaagctcat
     4921 gggcatacac cagcgtgaat taaaatcaac tattgttttt tcagggtcaa ggattttgaa
     4981 aacctgtttt ttgtaggttt cagcattgct caacacttct tcacgggtca gtcttttacg
     5041 ag
//
LOCUS       DAOSZP010000092         5027 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_13895_length_5027_cov_2.789622, whole genome shotgun sequence.
ACCESSION   DAOSZP010000092 DAOSZP010000000
VERSION     DAOSZP010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5027
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13895_length_5027_cov_2.789622"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..398
                     /locus_tag="PK348_03060"
     CDS             <1..398
                     /locus_tag="PK348_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cAMP-binding protein"
                     /protein_id="HXK65224.1"
                     /translation="MVQTQPQLATRLIQLLSERIWTAYRQLENLMIRDPLGRMYDTLL
                     IQVEKQKVRIAPKESFTFDFGVKELLNMVGIPQDKGDHLVVELLEDKNITLDEGKLIC
                     TNLEELEKTVNFYKKKSALERKREASKSS"
     gene            376..2472
                     /locus_tag="PK348_03065"
     CDS             376..2472
                     /locus_tag="PK348_03065"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HXK65225.1"
                     /translation="MKPAKVVKYSAIILILIIMLLAAFAAAFVYLFPKETLKSIIVQT
                     LGDSLNRPVTIGAIDYSIRGIQITDLYIYDTDKEKPYIAHSKDAAIRFQLVPLLQKQF
                     IINYISLNDAIIHILYYTENKITASNLEKLLKELLSKEKSTITTQIKLISLNNTTVIL
                     ENPPQKLKPLEGKYSIDTHVELHDDILELKNLSINLPQNRGKISGDCSITITSNYKIQ
                     GDFTLRDCDLLWVYNWGKNLATILPYRVFSGTIDNLLITKSAVQGTVTGSCKLSNNAM
                     LFLSNAFTNVNLNKKTVTVANAKGKIENSTFVLNQLLFAFSGEIQNIKVSDASINTKE
                     IAPLLGKIDISDIAGELKGNFEYKDNSIDADVTLSNLSIGKKGTILSLQETNLLIKNN
                     QFKKDGIQATVYSVPCVLSVATVDSSFEKFTVNIEADEVDLNKIISRQPQKPEQPEKT
                     EKEIHIPYELSGKCIIQKLYKDNLVIEKINCNYTMSKNIIALPTFSAKVFGGDITGRS
                     VITLSSENPQITLVAKFSNARLQNISSYFQEYENRLFGNISGQLQVTIVPKDPVIDRI
                     TGKVEVSVENGKLANTGLQNGLSIWLSDLKYKLSNLEFQKIYGNFNLSGPAITINSFI
                     FNAPDIRLKLNGQYNRHDESDIAISLEFSSNFIQDLPNPALLQLAKYKKGRWYIIPFR
                     AQGKDIFEGKNIKQVQ"
     gene            complement(2474..2968)
                     /gene="leuD"
                     /locus_tag="PK348_03070"
     CDS             complement(2474..2968)
                     /gene="leuD"
                     /locus_tag="PK348_03070"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013291143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HXK65226.1"
                     /translation="MKAHGKVWKFGNDINTDEIIPARYLNTSDPDELAQHCMEDADPD
                     FMKKARPGDIIVAGKNFGCGSSREHAPIAIKAAKISCVIAQSFARIFYRNSVNIGLPI
                     IESEEAAKDIEQGDEIEIDFDSGVIKNITKNKEYRAKPFPPFMQDIIAKGGLMAKVKA
                     ELKK"
     gene            complement(3009..4268)
                     /gene="leuC"
                     /locus_tag="PK348_03075"
     CDS             complement(3009..4268)
                     /gene="leuC"
                     /locus_tag="PK348_03075"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HXK65227.1"
                     /translation="MGMTISEKILAAHAGKERVEPGELIEAKVDLVLGNDITAPIAIK
                     EFEDLGVSKVFDNEKIALIPDHFTPNKDIKSAQQVKILREFAKKYNIVHFYDVGRVGV
                     EHALLPELGLVRPGMCIIGADSHTCTYGAFGAFSTGVGSTDMAAAMATGKTWFKVPES
                     IKFVVYGKLQPYVSAKDIILHIIGSIGVDGALYKSMEFTGDTIAELSMEGRMTMANMA
                     IEAGAKNGIFAPDGKTVEYIKSRTGLPYTLYYSDDDARYVEIKEYDASKIEPTVAFPH
                     LPENAKPAREAGIPIDQVVIGSCTNGRIEDLRVAAKILKGRKVHKDVRLLIIPATQEV
                     YRKALEEGLIDIFIEAEAAVSAPTCGPCLGGHMGILAEGERCVATTNRNFVGRMGHPK
                     SEVYLASPAVAAASAILGRIAHPDELR"
BASE COUNT         1600 a         1050 c          922 g         1455 t
ORIGIN      
        1 caatggttca gacacaacca cagcttgcaa ccaggcttat acagcttttg agtgagcgta
       61 tctggacagc ctatcggcaa ctggagaatt tgatgatacg tgatccgtta ggcagaatgt
      121 atgatacatt gcttatacag gttgaaaagc agaaggtaag gatagccccc aaagaaagct
      181 ttacttttga ttttggtgtt aaagagcttc tcaatatggt gggaattccg caggataagg
      241 gcgaccattt agtggtggaa ttacttgaag ataagaatat aacactggat gaaggcaagc
      301 tcatatgcac caatttagaa gagcttgaaa agactgtaaa cttttataag aaaaaatcag
      361 ctttagaacg caaacgtgaa gccagcaaaa gtagttaaat acagcgctat aatacttata
      421 cttataatca tgctgcttgc tgcttttgca gcagcatttg tctatctttt ccctaaagaa
      481 acattgaaat caattattgt acaaacactg ggggattcct taaacaggcc agttactata
      541 ggagcaatcg attatagcat cagaggtatt cagatcactg atctgtacat atacgacact
      601 gacaaagaaa agccgtatat tgcgcactca aaggatgcag caatcaggtt tcagcttgta
      661 ccactattac agaagcaatt tattattaac tatatttcac tcaatgatgc aataattcat
      721 atactgtatt atacagaaaa taaaataaca gcgtcaaatc ttgaaaaatt gcttaaagaa
      781 ttattatcaa aagaaaaatc caccataacc acccagatta aactaatatc actcaataat
      841 acaaccgtta ttcttgaaaa cccaccccaa aagctcaagc ccttagaagg gaaatacagt
      901 attgatactc atgtggaact gcatgatgat attctggaat tgaaaaacct ttcaataaac
      961 cttccgcaaa acagaggaaa aatttccggt gattgttcca taactattac cagtaattat
     1021 aaaattcagg gcgatttcac cctgcgtgat tgtgatctac tatgggttta caactgggga
     1081 aagaatctgg caactatatt gccttacaga gtattttcag gaactatcga caaccttttg
     1141 ataacaaaga gtgctgttca ggggactgta accggcagtt gcaaactcag taataatgca
     1201 atgctttttc ttagcaatgc gttcaccaat gttaatctta ataaaaaaac tgtcacagta
     1261 gcaaatgcaa aaggaaaaat tgaaaattca acgtttgtac tgaaccagtt gctgtttgct
     1321 tttagtggag aaatccagaa tatcaaagta agtgatgcat caattaatac aaaagagata
     1381 gctccattac ttggaaaaat tgatataagc gatattgctg gcgaacttaa aggaaatttt
     1441 gaatataaag acaatagtat tgatgctgat gtaacgctat caaacctttc tattgggaaa
     1501 aaaggtacta tactatcatt acaggaaaca aatcttctaa taaagaataa tcaatttaaa
     1561 aaagatggca tacaggcaac cgtatattcc gtaccctgtg tgttatctgt tgctactgta
     1621 gattcatcat ttgaaaaatt tacggtaaat attgaagctg atgaagttga cctcaataag
     1681 attatttcca ggcagcccca aaaacctgaa caacctgaaa aaacagaaaa agaaatacat
     1741 ataccgtatg aactatcagg aaaatgtatt atacaaaaat tatacaaaga caacctggtt
     1801 attgaaaaaa tcaactgcaa ctataccatg tcaaaaaata tcattgcact acccacattt
     1861 tctgctaaag tatttggtgg agatattacc ggtagaagcg tcatcaccct ttcatcggaa
     1921 aaccctcaga tcacactggt tgcaaaattt agtaatgcac gcctgcagaa tataagctca
     1981 tactttcagg aatatgaaaa cagactcttt ggcaatatat caggacaatt gcaggtaact
     2041 atcgtaccca aagatcctgt tattgataga ataaccggca aggttgaagt gtcagttgaa
     2101 aatggcaaac ttgcaaacac cgggttacag aatggactga gtatatggct cagtgattta
     2161 aaatacaaac tgagcaatct ggagtttcag aagatatatg ggaattttaa tctcagtggc
     2221 cctgctatta ccatcaattc atttatcttt aatgcacctg atatacgact aaaactaaat
     2281 ggtcagtaca accgccatga tgaaagtgat atagctatat cactggaatt cagttcaaac
     2341 tttatccagg atctgcctaa ccctgctctg ctgcaacttg caaaatacaa aaaaggccgg
     2401 tggtatatca ttccattcag ggcacagggt aaagatatct ttgaaggcaa aaatataaag
     2461 caggtgcaat aacttatttt ttcagttcgg ccttaacctt ggccatcagg ccaccttttg
     2521 caatgatatc ctgcataaat ggcggaaatg gctttgccct gtattcttta ttttttgtaa
     2581 tattttttat aacgcctgaa tcaaaatcaa tttcaatctc atcaccctgt tcaatatcct
     2641 ttgctgcttc ctctgactca atgatgggaa gaccaatgtt cacgctattt cggtaaaata
     2701 ttcgggcaaa ggactgtgca atgacacagg aaatctttgc tgcttttata gctatgggag
     2761 catgttcccg cgatgagcca catccaaaat ttttaccggc aacaataata tcacctggcc
     2821 ttgccttttt catgaaatca gggtcagcat cttccataca gtgctgtgcc agctcatcag
     2881 ggtcggaagt attcagatat cgtgcaggga taatttcatc ggtattaata tcatttccaa
     2941 atttccaaac tttaccatgt gctttcatgc tgtacctcgt gaatagtttc aattagcgat
     3001 agctcatgtt atcgtaactc atctggatga gcaatccttc ccagtattgc agatgctgct
     3061 gctaccgcag ggcttgctaa atacacttcg ctttttggat gccccatacg gccaacaaag
     3121 tttcggtttg tggtggcaac acaccgttca ccttcggcta aaattcccat atgtcccccc
     3181 aaacatggcc cgcatgtggg agcagatact gccgcttcag cttcaataaa tatatctata
     3241 agaccttctt ccagagcttt tctatatact tcctgtgttg caggaatgat gagcaatctg
     3301 acatctttat gcactttgcg gccttttaat atcttagctg ccacccgcag gtcctcaata
     3361 cgcccgttgg tacaggagcc aataacaacc tgatcaattg gtatccctgc ttcgcgagca
     3421 ggtttagcat tttcaggcag atgcggaaac gctaccgttg gttcaatttt tgaagcatca
     3481 tattctttaa tttccacata gcgtgcatca tcatcgctgt aatacagtgt atagggaagg
     3541 cctgtgcggc ttttaatata ttcaacagtc ttaccatcag gggcaaagat accatttttt
     3601 gcaccggcct caattgccat attagccata gtcatcctac cttccatgga taattcagca
     3661 atagtatcgc cggtaaattc cattgactta tataatgcac cgtcaacccc aatgctgcca
     3721 ataatatgca aaatgatatc ctttgctgat acatacggtt gcagtttgcc atacactaca
     3781 aatttgattg attcgggcac cttgaaccat gttttgcccg tggccatcgc tgcggccata
     3841 tcagtggaac ctacaccggt cgaaaatgca ccaaacgcac cataggtaca ggtatgtgaa
     3901 tcggcaccaa taatacacat accggggcgc accaaaccca attctggaag cagagcatgt
     3961 tcaacaccaa ccctgcccac atcataaaag tgaacaatat tatatttctt tgcaaactct
     4021 ctcagtattt taacctgctg tgcagatttt atatccttgt taggagtaaa atgatccggg
     4081 ataagcgcta ttttttcatt atcaaaaact tttgatacac ctaaatcttc aaattcttta
     4141 attgcaatag gggcagtaat atcattaccc agtactaaat ccactttagc ttcaattaat
     4201 tcacctggtt ctacacgttc cttcccggca tgtgctgcta aaatcttttc agaaattgtc
     4261 atgcccatac tatttttcct ctacatagat atttacaatt acatgcaatt gcaatagtaa
     4321 aaatgtatca tccttttata tattttcagt ttttttatgg cagttttggc gatagttact
     4381 gttaatatac tatgcaggga ttgtctataa aaaatggtcg ttgagagaat tccataaaca
     4441 ttattttatc ataatcttct catctggata taatgcactc atctcgatac gccttgaagg
     4501 ctactcgatg accacatctc gatacgcctt gaaggctact cgatgaccac gtctcgatac
     4561 acaaccggtc actgagtgcg tgcgcagcac gtgtatcgaa gtgaccggag tgctgttgtc
     4621 tcgatacgcc cttgtttgcc atgtatcgaa gcaccggttg ccgagtgcac gcgaagcgtg
     4681 tgcatcgagg caccgattcc cgaaacttct cgctactctc gaatgatagg gcaaggattt
     4741 gccgtgcagg gaagcacggc aaatttggat gcgggcgagg acctgaagga tgctattccg
     4801 cagcccgcag tggaaagttt aatattacag agagtaaagg gcatttttct ttggtacttt
     4861 cttttgttgc ccgggacaaa agaaagtaca taaaagcatt atcattttaa aggaataaag
     4921 cgactgagaa atcttaccct atggtttgtt ttgatacaac gccttcttat aaattataca
     4981 ttctctacaa gaactgcacc acccattcca ccaccaacac acagggt
//
LOCUS       DAOSZP010000093         5016 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_13931_length_5016_cov_3.721427, whole genome shotgun sequence.
ACCESSION   DAOSZP010000093 DAOSZP010000000
VERSION     DAOSZP010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5016
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13931_length_5016_cov_3.721427"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            479..1240
                     /gene="tpiA"
                     /locus_tag="PK348_03080"
     CDS             479..1240
                     /gene="tpiA"
                     /locus_tag="PK348_03080"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010047578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HXK65228.1"
                     /translation="MNTKRKQIFAGNWKMYTTQKEAVNLALGIAEGLQKTDREIVIFP
                     PSVYVVCVKAACEGTPIKVGFQNMYFEKEGAFTGEIGPLMVKDTGASYVLIGHSERRH
                     IFGETNEMIHKKVVAAIAHGITPMLCVGELLEEREAGKSESTVETQIVKAFEGLTAED
                     ALKVVIAYEPVWAIGTGKVATPEIAQSMHAFIRKTLQTLYTDTVAETIPVLYGGSVKP
                     DNIAGLYAMPDIDGVLVGGASLKVQSFLDIIQVAL"
     gene            1294..1629
                     /gene="secG"
                     /locus_tag="PK348_03085"
     CDS             1294..1629
                     /gene="secG"
                     /locus_tag="PK348_03085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HXK65229.1"
                     /translation="MSILISIGTVLFVILSILLIIIILLQSDKSAGMGILGGSSNTAF
                     GSSTADIITKITTVMVALFMLGSLGLSVVESYRARSLEKDLLSEEKKGGVIEQKALEK
                     PQEQPQQPK"
     gene            1692..1883
                     /locus_tag="PK348_03090"
     CDS             1692..1883
                     /locus_tag="PK348_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011734413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HXK65230.1"
                     /translation="MAKEVYVDQSECTGCELCVDTLPEVFEMTPDGVSKVKNSKGASE
                     DKIQEVIDSCPAECIHWKD"
     gene            2009..3160
                     /gene="metK"
                     /locus_tag="PK348_03095"
     CDS             2009..3160
                     /gene="metK"
                     /locus_tag="PK348_03095"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011733999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HXK65231.1"
                     /translation="MGRNYLFTSESVSEGHPDKVADQISDAILDEHIKGDPYSRVACE
                     TLVTTNRVVISGEITSAVTVDYEAIARKVIAEIGYTSPEIGFDAKNCIVEVFIHPQSP
                     DISQGVTEGQGLFKEQGAGDQGMMFGYAVAETDELMPMPILYAHRLVKRLSEIRKKGE
                     VNFLRPDGKSQVTVEYENDKPKRISAIVISTQHDDAVTLPTLEEMVRELVIKKVIPPH
                     LWDSNTTIYVNPTGRFEVGGPHGDTGLTGRKIIVDSYGGMGRHGGGAFSGKDPSKVDR
                     SAAYMGRYIAKNIVAAGLAYRCELQVAYAIGVADPVSVMVDTFGTNTIPEEEIEKRIK
                     AIFNLKPKSIIQTLDLLKPKYKATAAYGHFGRNESGFTWEDTKVAAQLR"
     gene            3338..3889
                     /locus_tag="PK348_03100"
     CDS             3338..3889
                     /locus_tag="PK348_03100"
                     /inference="COORDINATES: protein motif:HMM:NF021886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HXK65232.1"
                     /translation="MSIKYAILGLLHYTDMHGYRIKDHIEKNFGHMWTINFGQIYTSL
                     KDLEESGLIEVTEIVPSDDGGPHKKCYRITEEGKKDFTRWLHSSPEKQMLLRDPFLLR
                     FAFFGFGDPDRAVEIIDDQIKMYEEQLERRTQNLSRWRKQSVYVRLIADLGVKFNEMY
                     LEWLKQAKEEIAKESEELFVKKV"
     gene            3927..4919
                     /locus_tag="PK348_03105"
     CDS             3927..4919
                     /locus_tag="PK348_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015681799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HXK65233.1"
                     /translation="MIQYKGITLVTGAAGFMGSHLVEYLAKQGVKVRATSRPRKDTSF
                     FDNLDVEFVPSDLTKPETLPKLFEGDVDRVFHLGAICNFSTPYEVLYHTNVEGVDRIT
                     TLALAHKVKCYVHVGSTSVYGYYKGVPFTEDSPREPQDSYGRSKRDGENIVWGKIKQG
                     LPAIITRPCTVYGPRCNDGAGKVFSRPTRIGAIPGNGRQLLSNVRAEDVAAAVVHLSQ
                     LKEAIGQAYNISDDSHPTVEEALCLAAKVFETTPPRVHMPLPIIKLAARIDGYFSARK
                     GKIPDLEYDAVKYLYDDYVVDNTKLKSTGFRFQYPDFKQSMEQIGEWYKRQKSR"
BASE COUNT         1649 a          820 c         1118 g         1429 t
ORIGIN      
        1 gaatgacaaa gaaataacat aagatttctc ctcactgcgt tcgtcgaaat gacaaggtaa
       61 aaacattcgt tgtcgaaatg acaaggtaag agcattcgtt atcaaaataa tagtgtacaa
      121 atattgtggt attcatctat tccgacatgt catttcgaat ccccgattgc atcggggtta
      181 aaataagttt tctccttaca aagattaaac ggtcgcgttc tgttttcagc ctgaagtaag
      241 attaaacacc tgcgactgtt atcaggaact atcgccccaa gtatatgacg attactatgt
      301 tacatgtatt ataaaaattt ctttgcaacc ttttcatagg tatgataatc attgataatg
      361 acatataatg tccctttaaa aaattttgta aaatcgttgc atttgtgaaa aaatatagta
      421 tctgtggttt atagattgag gattattgca tggaaaaata acacaaaaag ggaacattat
      481 gaatacaaaa cgaaaacaga tatttgctgg aaactggaag atgtatacca ctcagaagga
      541 agcagtaaac cttgctctgg gtattgctga aggccttcaa aaaactgaca gggagatagt
      601 gatttttcct ccatcggtgt atgtagtgtg tgtcaaggct gcctgtgaag gaacgcctat
      661 taaggttggt ttccagaata tgtattttga aaaggaaggt gcctttacgg gggagattgg
      721 gcccttaatg gttaaggata ctggagcttc ttacgttctt attggtcact cggaacgcag
      781 gcatatcttt ggtgaaacca atgagatgat acacaaaaaa gtagtggcgg cgatagctca
      841 tggaataacg cccatgctct gtgtaggtga acttttagaa gaacgtgaag cgggaaaatc
      901 cgaatcaact gttgaaacgc agatagtaaa agcctttgaa ggattaactg cagaagatgc
      961 gctgaaagtg gttattgcct atgagcctgt gtgggcaatt ggcacaggca aagttgcaac
     1021 acctgaaata gcacaatcca tgcatgcttt tattagaaaa acattgcaaa cgctgtatac
     1081 tgataccgtg gcagagacta tcccggtgtt gtatggtggt tctgttaaac cggataatat
     1141 tgcagggttg tacgcaatgc ctgacataga cggtgtgctg gttggtggtg caagccttaa
     1201 agtacaatct tttcttgaca ttatacaggt tgcgttgtaa caaggcacaa ataatagcat
     1261 ttttaaaatt tattgaatat ataaaggtgc agtatgagca tacttatttc aatagggaca
     1321 gtattgtttg taatattatc aatactgctc attatcatca tcctgctgca gtcggataaa
     1381 agcgccggaa tgggtatttt aggtgggtca agtaacacag catttggttc ttcaactgct
     1441 gatattatca ccaaaattac cactgttatg gttgcccttt ttatgctagg atcgcttggg
     1501 ctttcagttg tagagtcata cagggcgcgc agcctggaaa aagacctttt gagtgaagaa
     1561 aagaaaggtg gtgttattga gcaaaaggct ttagaaaaac ctcaagaaca gccccagcag
     1621 ccaaaataaa ttgcaaatgg atatcctttg tagtataatg ctataaatat cttaaaccaa
     1681 tggaggaacc tatggcaaaa gaagtgtatg ttgatcagag cgaatgtaca ggatgcgaac
     1741 tttgtgttga tacattgcca gaagtttttg aaatgacacc tgatggtgta agtaaagtta
     1801 aaaattcaaa aggtgcatca gaggataaaa ttcaggaagt tattgatagc tgtccagctg
     1861 agtgtattca ctggaaagac tgatagatca ctatatatat attaaattct aaccatactt
     1921 acaaggcata ttcatgcaat atgcctttca atgtttatag aagtacgggt tgggcaatta
     1981 ttgtaagcgc aaaggagtaa acataacaat gggaagaaat tatttattca catctgaatc
     2041 tgtttcggaa gggcatcctg ataaagttgc cgatcagatt tctgatgcca ttttagatga
     2101 acacataaaa ggtgatccat actcacgtgt tgcctgtgaa acgctggtta ccaccaaccg
     2161 tgttgttatt tcaggcgaga ttacctcagc ggttactgtt gattatgagg caatagcacg
     2221 caaggttatt gctgaaattg gctatacgtc acctgaaatt gggtttgatg caaaaaattg
     2281 tatagtagaa gtatttatac atccacaatc ccctgatatt tcgcagggtg ttactgaagg
     2341 ccagggactt tttaaggaac agggtgctgg tgatcaggga atgatgtttg ggtatgcggt
     2401 tgctgaaacc gatgaattga tgcccatgcc cattttatat gcacaccgcc ttgtcaaacg
     2461 gttatctgag atccgcaaaa aaggtgaggt gaacttcctg cgccccgacg gaaaatctca
     2521 ggttactgta gaatatgaaa atgataaacc caaacgcata agtgcaatag tcatttcaac
     2581 acagcatgat gatgcagtca cgttgcccac actggaagaa atggtccgtg agcttgtgat
     2641 aaaaaaggta attcctccac atttgtggga tagcaatact acaatatatg ttaacccaac
     2701 aggccgcttt gaagtaggtg gacctcatgg ggataccggt ttaacgggaa ggaagattat
     2761 tgtcgatagt tacggtggaa tgggcagaca tggtggtgga gcattttcag gcaaggatcc
     2821 atcaaaggta gaccgatctg cagcatatat gggacgctat attgccaaga atattgtggc
     2881 tgcagggctt gcataccgat gtgaattgca ggtggcctac gctattggtg ttgcggatcc
     2941 agtatcagtg atggttgata cgtttggaac taataccatc ccggaagaag aaattgaaaa
     3001 acgaataaaa gcaatattta atttaaaacc aaaatctatt attcaaaccc ttgatctgct
     3061 taaaccaaag tataaagcaa cagcagcata cgggcatttt ggtcgtaatg aatcagggtt
     3121 tacctgggaa gacacaaagg tggcagccca gttgcggtaa agaagaattt aattttaaaa
     3181 ctttgaagag tatgaaaagg aatgcaggaa gtgcattcct tttttttatg gataaaattt
     3241 ttctaaaaaa aatccaaaaa aaatacttga cactatatat aaagtatata tatataatgt
     3301 atatagttaa aatttaatat ttgtaaagga tatctacatg tctataaaat atgctattct
     3361 gggtctgctt cattatactg atatgcatgg ctatcgcata aaagaccata tagaaaagaa
     3421 ttttggccac atgtggacaa ttaattttgg gcagatatat accagtttaa aagatcttga
     3481 ggaaagcgga ttaattgaag tcactgagat tgttccgtca gatgacggtg ggcctcataa
     3541 aaagtgctat cgcataacag aagaagggaa aaaagatttt acaaggtggc ttcattcatc
     3601 gcctgaaaaa caaatgctgc tgcgagaccc ttttttgttg cgttttgctt tttttggatt
     3661 tggagatccc gatcgggcag tggaaatcat tgatgatcag ataaaaatgt atgaggaaca
     3721 gcttgaacga agaacacaaa atctttcacg ctggcgaaag cagagtgtat atgtcaggct
     3781 gatagctgat ctgggagtta agtttaatga aatgtattta gaatggctga aacaggctaa
     3841 agaagaaata gcaaaagaat cagaggagct ttttgtaaag aaagtataac atatcaatac
     3901 agttggtgta ttgcctggag gaaagtatga tacagtataa agggattaca ctggttactg
     3961 gtgctgcagg atttatggga agccaccttg ttgaatatct ggctaaacag ggtgttaagg
     4021 tgcgtgcaac atcacgacct cgcaaggata cttcattttt tgataattta gatgttgagt
     4081 ttgtcccttc cgatttaaca aaacccgaaa cactaccaaa actttttgaa ggtgatgtag
     4141 acagggtttt tcatcttggt gctatatgta atttttccac accctatgaa gttctttatc
     4201 ataccaatgt agaaggtgtt gacaggataa ccacactggc acttgcccat aaagttaaat
     4261 gttatgttca tgtaggttca acaagtgtat atggatatta taaaggtgtt ccctttactg
     4321 aagacagccc acgtgaacct caggattcgt atggcagaag taaacgagat ggagaaaata
     4381 tagtctgggg aaaaataaaa cagggacttc ctgcaatcat tacaaggcca tgtacagtat
     4441 atggtcctcg ctgtaatgat ggtgctggca aggttttttc acgaccaacc aggattggtg
     4501 caattcccgg aaatggcagg cagttattgt caaatgtacg tgcagaagat gtagcagctg
     4561 ctgtagtgca tctttcacag ctaaaagaag ccatagggca agcatataac atttcagacg
     4621 attcacatcc aacagttgaa gaagcattat gtttggcagc aaaggtattt gaaaccacac
     4681 cgccacgagt tcacatgcca ttgcccatta tcaaattagc tgcaagaatt gacggttatt
     4741 tttcagccag aaaaggcaaa atacccgatc tggagtatga tgctgttaaa tatctttatg
     4801 atgattatgt tgtggataat acgaaattaa aatctaccgg gttcaggttt caatatcccg
     4861 attttaaaca atcaatggaa caaataggtg aatggtataa acggcaaaag agcagataaa
     4921 ggttaaaaag aataatataa ataataatta ttaaattgga ggaacgtata tgaatgatgt
     4981 tatgagctat gaattgagcc ctatagtaaa tacaaa
//
LOCUS       DAOSZP010000094         4991 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_14021_length_4991_cov_3.182942, whole genome shotgun sequence.
ACCESSION   DAOSZP010000094 DAOSZP010000000
VERSION     DAOSZP010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4991
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14021_length_4991_cov_3.182942"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..612
                     /locus_tag="PK348_03110"
     CDS             <1..612
                     /locus_tag="PK348_03110"
                     /inference="COORDINATES: protein motif:HMM:NF014135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HXK65234.1"
                     /translation="DYKYNLSSMIFSVPIFILITLFFLLHHVLDISIPLIALTGSSLI
                     LLLGKIKPSKIIKEIDWVLLLFFASLFIVVKGAEKAGILSLFLKFSNFNNSLHSITLL
                     HAVSLVFSQIVSNVPFVILLGPTLKYSNSNIIWLALASSSTLAGNATIIGAMANLIVL
                     ESAAKSGIQISFMEFLKPGIIVTVLTLMLSIAVLYFYSVANSL"
     gene            759..1100
                     /locus_tag="PK348_03115"
     CDS             759..1100
                     /locus_tag="PK348_03115"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HXK65235.1"
                     /translation="MQIDVHKEGPLLIFTLNGRYDISEVHNFETVYRHHIEDNPKIIA
                     LNLRDLKYIDSSGIGSLVRCMNLALKSNIEFVCYDINENVESIFKIAKLDQFISILTQ
                     EEFIKKYIKKQ"
     gene            complement(1087..2079)
                     /locus_tag="PK348_03120"
     CDS             complement(1087..2079)
                     /locus_tag="PK348_03120"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HXK65236.1"
                     /translation="MGVANLFTYKYLKRKKLTVIIYLIILCAIVTINVLFLLAHQFAL
                     NYIAISFFSEIMLLILSNIVAVSISFVIVNKMLQKRYNDVAASLLTVLKQFEKGNFTY
                     PFSIDNGRFDNVLRYMQKLMMRQNSTPASFDEKLNERVFEIITVLISAMEDKDPYTSG
                     HTTRVAHYALAIGKRLGLSQEKMRLLNHAAAAHDIGKIGIPIEIIEKNGRLNEKEKLM
                     MESHPIRGMKILSSIDYLEDIASIIMYHHERADGKGYYQLPGEKVPVEAKIIAVADAL
                     DAMLSDRPYRKALSLYEAKQEILKNKGTQFDPEVVDALINVLEEKEPLKEFIAF"
     gene            2268..3242
                     /gene="trpS"
                     /locus_tag="PK348_03125"
     CDS             2268..3242
                     /gene="trpS"
                     /locus_tag="PK348_03125"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006718952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HXK65237.1"
                     /translation="MSRILSGIQPSGTLHIGNYFAMMKPMIELQHNNDLFCFIVNYHA
                     LTSIHNPQELSENTINAAMDFIALGLDPDKVHFWVQSDIPEVTELAWILSCHTSLGLL
                     ERSHSYKDKIAKGIVPNTGLFTYPVLMAADILLFNAEVIPVGKDQKQHLEITRDIAER
                     FNQMYGEIFVLPEPMISEDIAVIPGIDGQKMSKSYNNTINIFEDEATLKKKVMRIVTD
                     STPVEAPKNPDTCNLFALYKLFAEPAKTEILKERYRKGGIGYGEVKKELFGMIWEYFA
                     PFRDKRKQLQNNKSEIAAILKKGAEKTRPIAQETLLRVKKAVGLDYLQ"
     gene            complement(3243..4640)
                     /locus_tag="PK348_03130"
     CDS             complement(3243..4640)
                     /locus_tag="PK348_03130"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HXK65238.1"
                     /translation="MKLRIVLITVITFVICTAPLTDAKTVKKAQGSENISDLSKFNEQ
                     LVKVTNSAIPTVVSISAEKKAGSPRESKRMPMPFPVPKEAKALGSGVIIDKRGYIVTN
                     NHVVKNTKSITITLFDKRTFPCELVGSDPATDIAVIKISGTVPQDLPVAVMADSDTLK
                     VGEIAIAIGNPFGFGHTVTMGIISATGREGFGLADYEYFIQTDAAINPGNSGGALINI
                     NGKLIGINTAIFSRNGGYMGIGFAIPSNMVKQVVDELINKGKVTRGWLGVYIQNITPE
                     IAKNFKFEGRKGVLVADIMKDSPAKNAKIEIGDIIISVNGVEVSDINQLRRYVASLKP
                     GSTAKVKIFRGGQELEREVLIAELPAQAQAPEEKMEEVDTIGMRVGDITEENAYKFRI
                     TDTTGVIVLEIQEQSPAYEAGMMVGDIIKEIENTPVENVDKYNELLKQYEKKETLLLL
                     VKRGGIHKFVVIKKQ"
BASE COUNT         1569 a          957 c          810 g         1655 t
ORIGIN      
        1 gactacaaat ataacctgtc atcgatgata ttttctgttc caatttttat tcttatcaca
       61 ttattttttc tgcttcatca cgttcttgat atatcaatac cactgattgc gctgactggt
      121 agttcattaa ttttattatt gggtaaaatt aaaccatcaa aaataatcaa agagatagac
      181 tgggttttat tattgttttt tgcatcacta tttatagtag tcaaaggtgc tgaaaaagct
      241 ggaatcctgt cattattcct gaagttttcc aactttaata acagcctgca cagtataacc
      301 cttttacatg ctgtcagtct ggttttttca cagatagtca gcaatgtacc ttttgttatt
      361 ctattagggc caaccttaaa atacagcaac agcaacatca tatggttagc gctggcttca
      421 tcatcgacac tggctggcaa tgcaacaata attggtgcaa tggccaattt gattgtgctg
      481 gaatcagcgg caaaatcagg tattcaaata agctttatgg aattcttaaa acccgggata
      541 attgttacag tattaacatt aatgctatca atcgctgtac tatattttta ttcagtagct
      601 aactcactgt gaccaatatt ttttaatctt ttaaataatt tatttgttga catgtataat
      661 tcaatacctt aacctaaata acaagaattt tgcagttata aaacatatat atcaggtttt
      721 tttactgttg ctaattcata atttatttga gttatcacat gcaaattgat gtacacaaag
      781 aaggaccctt attaatattt acgttaaatg gtcgatacga catttctgaa gtgcataact
      841 ttgaaacagt ttatcgtcat catattgaag ataatccaaa gattattgca cttaaccttc
      901 gtgacttgaa atatatagat tcttcaggta ttgggtcgct ggtacgatgc atgaatttag
      961 cacttaaaag caatattgaa ttcgtctgtt atgatataaa tgaaaatgtt gaaagtattt
     1021 ttaaaatagc taaactggac cagtttattt ctatcttaac acaggaagaa tttataaaaa
     1081 aatatatcaa aaagcaataa actcttttaa tggttctttt tcttccagca cattaatgag
     1141 tgcatcaacc acttcaggat caaactgtgt acctttattt ttaagtattt cctgttttgc
     1201 ttcataaagc gatagtgcct ttctatacgg cctatcactg agcattgcat ccagtgcatc
     1261 agcaactgca ataatctttg cttcaacagg aactttttca ccaggaagtt gataatagcc
     1321 tttgccatct gcacgttcgt gatgatacat tattattgat gcaatatctt caaggtaatc
     1381 aattgatgat aaaattttca ttccccgtat aggatggctt tccatcatga gtttttcttt
     1441 ttcatttaga cggccgtttt tctctattat ttcaatgggg atgccaattt taccaatatc
     1501 gtgggcagct gcagcatgat taagtaatcg cattttttcc tgtgacaaac ccaaacgttt
     1561 tccaattgcc aatgcatagt gtgcaacacg tgtggtatgt ccgctggtat aaggatcttt
     1621 atcctccatt gcactgatta acactgttat tatttcaaac actcgttcat tcagcttctc
     1681 atcaaatgat gccggcgtgc tgttttgtct catcattaat ttctgcatgt agcgcaacac
     1741 attatcaaaa cgaccattat caatagagaa tggataggta aagttacctt tttcaaactg
     1801 tttcagaaca gttaaaagcg aagcagcaac atcattgtac cgtttctgca gcattttatt
     1861 aacaatgaca aaagatatac tcactgccac tatgtttgac aaaatcaata acataatttc
     1921 tgaaaagaaa cttatggcaa tgtaattgag agcaaactga tgtgccagca aaaacagcac
     1981 attgatagta actatcgcac ataatataat taaatatatt atgactgtta atttttttct
     2041 tttaagatac ttataggtaa aaagatttgc cacacccatc gtaatgcctt tacactgtat
     2101 tcagtaatta taaaatactt aacatctttc caaatatcaa attattgtta tttttttata
     2161 agcgatagtt cctgttaaat taattattga cgataattgc atactcagta catctgttta
     2221 caatctgcat acacaacaac gtgaccatac tgaaaaaggg gttaaaaatg tcaagaatac
     2281 tttctggcat acaaccgtct ggaacgcttc atattggcaa ttattttgcc atgatgaagc
     2341 caatgattga actgcagcac aataacgacc ttttttgttt tattgtaaat taccatgcat
     2401 tgaccagtat tcacaatcca caggaactat ctgaaaacac cattaatgct gcaatggact
     2461 ttattgcttt aggtcttgat cctgataagg tgcatttctg ggtacaatcc gatattcccg
     2521 aagtgacaga gctagcatgg attttatcat gccacaccag cttaggactt ttagagcgca
     2581 gccacagtta taaggataaa attgcaaaag gcattgtgcc taacacaggc ctttttacct
     2641 atccagtgct tatggctgct gacatactgc ttttcaatgc tgaagttata cctgtgggca
     2701 aagaccagaa gcaacactta gaaataaccc gtgacattgc tgaacgcttt aaccagatgt
     2761 acggtgaaat atttgtgttg cccgaaccaa tgatatcaga ggatattgca gtcattcctg
     2821 gaatagatgg ccagaagatg tctaaaagct ataataacac cataaacatt tttgaagacg
     2881 aagccacact caagaaaaaa gtaatgcgta tagtaaccga ctcaacacca gttgaggcac
     2941 caaaaaatcc tgacacctgt aatctgtttg ccctgtataa attatttgct gaaccagcaa
     3001 aaactgaaat actcaaagaa cgctaccgta aaggtggcat tgggtatggt gaggtaaaaa
     3061 aagagttgtt tggaatgata tgggaatatt ttgctccctt cagagataaa cgaaaacaat
     3121 tacaaaacaa caaaagtgaa atagctgcaa ttttaaagaa gggtgctgaa aaaacccgcc
     3181 ccattgccca ggaaacacta ctgcgcgtta aaaaagcagt ggggctggat tatttacaat
     3241 aactattgct tcttaattac cacaaacttg tggatacctc cacgcttgac cagaagcagt
     3301 aatgtttctt tcttttcata ttgtttcagc aattcgttat atttatcaac attttccaca
     3361 ggagtatttt caatttcctt gatgatatca ccaaccatca tgcctgcctc atacgcagga
     3421 ctttgctcct gaatttcaag tacaattact cctgtagtat cagtaattct gaatttatag
     3481 gcattctctt cagtaatatc acccacacgc atgcctattg tatcaacttc ttccatcttt
     3541 tcttctggtg cctgtgcctg tgcaggaagt tcagcaatga gtacttcccg ttccagttcc
     3601 tgaccacccc tgaatatttt gactttggca gttgaacccg gttttaatga agcaacatat
     3661 cttcggagct gatttatatc agacacttca acgccattga ccgaaataat gatgtcgcca
     3721 atttcaattt ttgcattttt tgcaggtgaa tccttcatga tatcggcaac caaaacaccc
     3781 tttcttcctt caaacttgaa gttttttgct atttccggtg taatgttctg aatatatacg
     3841 ccaagccacc ctcgtgttac cttgccctta ttaatcaact catccaccac ctgttttacc
     3901 atgttggatg gtattgcaaa ccctattccc atatagccgc catttcttga aaatatagca
     3961 gtattaatgc caataagttt accattaatg tttatcaggg caccaccgga attaccggga
     4021 ttaatggcag catcggtttg gatgaaatac tcataatcgg caagtccaaa tccttcccgg
     4081 cctgtagcac tgataatacc catggttacg gtatgcccaa atccaaacgg attacctatt
     4141 gctattgcta tctccccaac ttttaatgtg tctgaatcag ccatgacggc aactggcaaa
     4201 tcctgaggaa ctgtaccgga aattttaatg actgcaatat cagttgcggg gtcagagcca
     4261 accaactcac aggggaatgt tcgtttgtca aataacgtaa tggttatgga cttagtattt
     4321 tttaccacgt gattgttggt aactatatat ccgcgtttat caataataac accagagccc
     4381 agtgcctttg cttctttggg gaccgggaaa ggcataggca tccgtttgct ttcccgtggg
     4441 ctgccagcct ttttttctgc tgatatactg acaacggttg gaatagcact attggttacc
     4501 tttaccagtt gttcattaaa ttttgataaa tcggaaatat tctcagatcc ctgagctttt
     4561 tttacagttt ttgcatcagt taatggtgcg gtacatataa caaaggtaat gactgtaatt
     4621 aagactattc gtaatttcat acaattatcc tgtttatata aaaaaatatt ctatcagaga
     4681 aataaacttt tctgactgta aatacagtat atacctataa acttatcggt atgtaattat
     4741 gctatcgtat attgttttgt tacaatattt acgataattg tcaagaatta tgaataataa
     4801 agtttgcatt attgccatct ttatttacag gaatacatgt gtccattttt tacaaaattt
     4861 atattcagta attattaata accaacttac agtgttaaat taatataata actaacctaa
     4921 attggaagaa attacttaaa tgaaaatatt taatatttta caaaaattcc agtaaagcga
     4981 tattttgtca t
//
LOCUS       DAOSZP010000095         4985 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_14036_length_4985_cov_3.825355, whole genome shotgun sequence.
ACCESSION   DAOSZP010000095 DAOSZP010000000
VERSION     DAOSZP010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4985
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14036_length_4985_cov_3.825355"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            364..1584
                     /locus_tag="PK348_03135"
     CDS             364..1584
                     /locus_tag="PK348_03135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65239.1"
                     /translation="MKRHVFWIALIMIISFVACGGSKPQQKASKAGCEMCGEADGFAA
                     IFDNDIALARDRAIDDAMNKLVKMKLGTTVEGRAIVEDYALVESIVEAKSTGMVRNWK
                     VIKEGSQEGAYTVRIYGEVYPQAVNDTIEATLKNYGRPKFMILVRETFEGQQNEPGFT
                     VTELTMMEIMQNAGFEFVDAATVQQLIKKDRAKMMQAMQGQVGGNVQELLLDDVGAEC
                     IIVGDVKTSDQSAVLKQYSANMKSKQAILNLKAIDVYTGSILASTSVNMPGAHIDDAT
                     ASKIAIQRALVKILGSTDEETGKFKSGPFMNQITKKFLLAATHRMITLNIVGLDYNGL
                     TKFRDVLQNRIRGIQKVYVRGQAGKQSKIDVEFSGKTTDLADELVGKSNSIGFKVEIK
                     ETYPNKIMMVVQKL"
     gene            1823..2188
                     /locus_tag="PK348_03140"
     CDS             1823..2188
                     /locus_tag="PK348_03140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65240.1"
                     /translation="MDSQEKQPERILAVDEISSILTSDSIVKALAQYKANTSEQEHAI
                     EFVKAHYDFIQEIVTNDIQRKIVRSDFEIKDLVSHVNALMQHKDEYIFTTLVVHSPRH
                     YQQVQKAVLQEMAKEEKEN"
     gene            complement(2292..4349)
                     /locus_tag="PK348_03145"
     CDS             complement(2292..4349)
                     /locus_tag="PK348_03145"
                     /inference="COORDINATES: protein motif:HMM:NF020660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1926 domain-containing protein"
                     /protein_id="HXK65241.1"
                     /translation="MDCNFIFCLHNHQPVGNFDHVFEWAYNDCYRKTLDLLYQYPEFK
                     FAIHNTGPLLEWIERHDPTYCDILAQMVQRGQVEIIGGGFYEPIFSIISEDDIHGQLS
                     LMQSFCKKRFGTEPEGFWTAERVWDPDIPQLVYDFKLQYTLLDDIHFRYAGIPESGMY
                     GYYITERLLRPLRVFPIDKFLRYSIPFKLPHETIDYFKRQCDKYGNCAFVYGDDGEKF
                     GVWPHTYQWVFEEKWLQNFIEAILKEDFITLVHPKDYIKKHPPIDRVYLTQGSYYEMS
                     EWALPAESAAELVRIHNEIKSAGREAEFYPFLKGGVWNNFLNKYHESNAINKRTLYLS
                     REITSLEMKTGKDLSQAKIELYKAQCNCAYWHGLFGGIYLTHLRGALYEHILESEELV
                     CKAKKLHTPEIVTGDFRNEGKDQIFIRNKSCSIVVNPAFGTSISEFSNRIKKVNAFDV
                     IARRKEAYHHLLAELNEDELNNDTVKSIHDMVTVKEKGLKRYLVYDSSRRYSCKELLF
                     TTMPSAEELMFNTIHYTDCSQYPYTYAIHNNSIISDSSGNPLPAITKIIHVHDTDPAI
                     SVHYTIRGFNGIVGIESNVNMRAPHAKECHYIVDGLAQEESYLDSMGQMNNITRYAIT
                     DDIRNVIFSVQASRTATLLRYPIYTVSQSDSGFEKNFQGSCVMLCFEVADTLELDLIL
                     SLE"
     gene            complement(4424..>4985)
                     /locus_tag="PK348_03150"
     CDS             complement(4424..>4985)
                     /locus_tag="PK348_03150"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HXK65242.1"
                     /translation="KLDSSKDFGKDIIPRIFSTERVFAYDYVQNEGERAYWRDIGTIA
                     SYYEAHRDIISVTPILNLYDKEWPIRTYMEQWPPAKFIHNEEGRRGVAINSLVANGAI
                     ISGGYVENSVISPGVKVNSFSEVKGSIIMHGSTVGRYAKIKNAIIDKDNHIPEHMHIG
                     YDLEEDKKHFTVSPEGIVVIPKGYLQ"
BASE COUNT         1520 a          941 c         1022 g         1502 t
ORIGIN      
        1 aaagatatat acaccccatc gcataaaacc atgcatgcca ccaacattaa agtatgattc
       61 cctggtaatc tggtcgacag gcaggtcata taaacttgaa aggctcatgt ataaccctcc
      121 ttacccttga aaataattgc acatattcat tttacaacaa tactttaata gttgaagtcc
      181 ttgtcaacac tattttaaat aaaatagtaa ctcaggaatg attgattagc aaccggtgat
      241 attctggaac ttacgttatg cagatattcg caaaaaatag tttgacttat ccaattaatt
      301 catgtaatgt atcaaatata tataactttc tacaaaaatt atttcaaaat acgaggttca
      361 actatgaaac gacatgtttt ttggatagca ttgattatga ttatttcttt tgttgcctgt
      421 ggtggttcca aaccacagca aaaagcgtct aaagcaggat gtgaaatgtg cggcgaggct
      481 gatggttttg ctgcaatatt tgataatgat attgcactgg caagggatag ggcaattgac
      541 gatgctatga ataaactggt taaaatgaag ttaggtacca ctgtggaagg ccgcgcaatt
      601 gtcgaggatt atgcactggt agaatcgata gttgaagcaa aatccaccgg gatggtacgg
      661 aactggaagg ttataaaaga aggttctcag gaaggggctt atacagtacg gatatacggc
      721 gaggtgtacc cacaggctgt taatgatact attgaagcta cattaaaaaa ttatggccgc
      781 ccaaagttta tgattttggt gcgtgaaaca tttgagggcc agcagaatga accgggcttt
      841 actgtaacag aacttaccat gatggaaatc atgcagaatg ctggttttga gtttgtggat
      901 gcagccaccg tgcagcagtt gataaagaaa gacagggcaa agatgatgca ggcaatgcag
      961 ggccaggttg gtggaaatgt tcaggaattg ctactggatg atgttggagc tgaatgtatt
     1021 atcgtaggcg atgtcaagac atctgaccag tcagcagtat tgaagcagta ttcagcaaac
     1081 atgaaatcaa agcaggcaat ccttaatcta aaggctattg atgtgtacac cggaagtatc
     1141 ctggcatcta ccagtgtgaa catgccgggc gctcatatag acgatgcaac tgcttccaaa
     1201 atagctatcc agcgtgctct ggtgaagatt ttaggcagta ccgatgaaga aaccgggaaa
     1261 tttaaatccg ggccgtttat gaatcagatt actaaaaagt ttttactggc agcaactcat
     1321 agaatgatta cactgaatat agtgggactg gactacaatg gcctgacaaa gttcagggat
     1381 gttttgcaaa atagaatccg gggcattcag aaagtttacg taaggggcca ggctggtaaa
     1441 cagtcaaaga ttgatgttga gttttcgggg aaaacgactg acctggcaga tgaacttgta
     1501 ggtaaatcaa atagtattgg ctttaaggta gagattaaag aaacctaccc aaataaaatt
     1561 atgatggttg tgcagaagct gtaatacaac atagaatttt ggattatatt tataagggca
     1621 gtgactatca ctgctcttat ttttggttga caatttattt tggtaccgta attttattgg
     1681 tagtactgtg tatagtctgc aatagccatg ataatgttgg aatgaagcaa cgctcaaaca
     1741 taccatctct ctgtgtgagg gatgaagcaa tgtcattctg tgggttgtga taatttaatt
     1801 tttatttccg ggggaacaat gtatggattc tcaagaaaag cagcctgaac gcattctggc
     1861 ggtagatgaa atatcatcaa ttttaaccag tgatagtatt gtaaaagcgt tagcacagta
     1921 taaagcaaac acttctgaac aagaacatgc aatagaattt gtaaaagccc attatgattt
     1981 tattcaggag atagttacca atgatattca gcgaaaaatt gtccgttctg attttgagat
     2041 caaagattta gtatcccatg taaacgcact tatgcagcat aaagatgagt atatctttac
     2101 tacgctggtt gtacattctc ccaggcatta tcagcaggtt cagaaagcag tgttgcagga
     2161 aatggctaaa gaagaaaaag aaaattaaag gtaatatatg ttaataattc ttaaaacacc
     2221 gtagatacat tgagtattaa tgtgttttta cataattcag aagatcgtca tatactgtaa
     2281 catgtaaata attattccag tgacagtatc aggtccagtt ccagagtatc agcaacttca
     2341 aaacataaca taacacagct tccctggaag tttttctcaa accccgaatc cgattgtgaa
     2401 acagtgtata tcggatatcg cagtaatgta gctgttctgc ttgcctgcac gctgaatatt
     2461 acattgcgaa tatcatcggt aattgcatac cgggtaatat tgttcatctg tcccatgctg
     2521 tccagatatg attcctcttg tgccaaacca tcaacaatgt agtgacactc tttggcgtga
     2581 ggtgcacgca tattgacatt agattctata cccacaatac cattaaaccc acgtatggta
     2641 tagtgaacgg atatggcagg gtcagtatca tgaacatgaa taatttttgt tattgctggc
     2701 aatggattgc cagaactatc gcttataatg ctgttattgt gtattgcata cgtataggga
     2761 tattgcgagc aatctgtata gtgtattgtg ttaaacataa gctcttctgc agaaggcatg
     2821 gttgtaaata aaagctcttt acatgaatat cgccgtgaac tgtcatacac aaggtagcgc
     2881 ttcaggcctt tttcttttac cgttaccata tcatgaatgg atttaacagt atcattgtta
     2941 agttcatctt cattaagctc agccaacaaa tgatgatatg cttctttgcg gcgagctatg
     3001 acatcaaaag cattaacttt tttgatacgg ttgctgaatt cagaaattga agtgccaaaa
     3061 gcagggttta caacaatgct acatgattta ttacggataa aaatctgatc cttgccttca
     3121 ttgcggaaat caccggtaac tatctccggg gtgtgtagct tttttgcttt gcatacaagc
     3181 tcttcgcttt caaggatgtg ttcatacaga gcgcctctta agtgtgtgag atagataccg
     3241 ccaaagagcc catgccagta tgcgcagttg cactgagctt tatacaattc tatttttgcc
     3301 tgtgacagat ccttgcctgt tttcatctcc agactggtta tttctcgtga aagatacagc
     3361 gtgcgtttgt taatggcatt tgattcatgg tatttgttta aaaaattatt ccacacacca
     3421 cccttcaaaa acggataaaa ctctgcttcc ctaccggcag attttatttc attgtgtatc
     3481 ctgacaagtt cagcagcgct ttcggcaggg agtgcccatt ccgacatttc atagtaactt
     3541 ccctgtgtga ggtataccct gtcaattggc gggtgctttt taatgtaatc ttttggatgt
     3601 accaatgtaa tgaagtcttc ctttaaaatt gcttctatga aattctgcag ccatttttct
     3661 tcaaataccc attgataggt gtggggccat acaccaaact tttcaccatc gtcaccataa
     3721 acaaaggcgc agttaccgta tttatcacac tggcgtttga aatagtctat tgtttcatgg
     3781 ggcaatttaa atgggatgga atagcgcaaa aatttatcta tagggaaaac gcgtaagggc
     3841 ctcagcagcc gttcggttat ataataccca tacattccac tttcgggtat tcctgcatag
     3901 cgaaaatgga tgtcatcaag cagcgtatac tgtaatttaa agtcatatac cagttgaggt
     3961 atatccggat cccatacacg ctcagctgtc cagaaccctt caggttctgt gccaaatcgc
     4021 tttttacaga agctctgcat taaggacagc tgtccatgga tatcgtcttc actgataatt
     4081 gaaaaaattg gttcataaaa accaccacca ataatctcaa cctggcctcg ctgcaccatc
     4141 tgtgctaata tgtcgcagta tgtcgggtca tgacgttcaa tccattcaag aagggggcca
     4201 gtgttgtgaa ttgcaaattt aaactcaggg tattgatata aaagatccag agttttccgg
     4261 tagcaatcat tgtaagccca ttcaaacaca tgatcaaagt taccaacagg ctgatggtta
     4321 tgcaggcaaa aaataaaatt acaatccaca gaattcccct ccccaactaa caattcatca
     4381 cgattcaatt aatctatcac ggaaaatgtt gtttgctata attttattgc agatagcctt
     4441 taggtattac cacaatgcct tcagggctaa cggtaaaatg ttttttatct tcttccagat
     4501 cgtatccaat atgcatgtgt tctggtatat gattatcttt gtctatgata gcgtttttta
     4561 tttttgcata ccgtccaaca gtagaaccat gcataatgat actgcccttt acctctgaga
     4621 aactgttaac tttcactcct ggagatatga ccgagttttc cacgtaaccc ccgcttatga
     4681 ttgcaccatt ggcaaccaat gagtttattg caacacctct tcttccttct tcattatgaa
     4741 taaattttgc cggtggccat tgttccatgt aggtacgtat aggccattct ttatcgtaca
     4801 ggttaaggat aggggttact gaaataatat ccctgtgggc ttcataatat gaggcgatag
     4861 ttccgatatc gcgccagtag gcgcgttccc cttcattctg tacgtagtcg tacgcaaaga
     4921 cacgctctgt agaaaaaatg cggggaataa tatctttgcc aaaatccttg gagctatcga
     4981 gctta
//
LOCUS       DAOSZP010000096         4984 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_14043_length_4984_cov_3.464192, whole genome shotgun sequence.
ACCESSION   DAOSZP010000096 DAOSZP010000000
VERSION     DAOSZP010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4984
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14043_length_4984_cov_3.464192"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1010)
                     /locus_tag="PK348_03155"
     CDS             complement(<1..1010)
                     /locus_tag="PK348_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013037318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HXK65243.1"
                     /translation="MTIFWVFVYLVIVAFLGYLGYRHTKGASDYLVAGRRVHPFIMAL
                     SYGATFISTSAIVGFGGAAGVFGMGLLYLTFLNIFVGIFIAFVFFGKRTRRMGYHLDA
                     HTFPEFLGRRFQSPFVQKFAGIIIFLFMPLYAAVVLIGAAKYIEQQFAIDYATALLFF
                     TLIVAVYVIMGGLKGVMYTDAFQGSIMAIGMLTLIIFTYYDLGGIIKSHADLTVLTPP
                     PPLAAKGHMGWTSMPALGSDFWWTLVSTIIMGVGIGVLAQPQLVVRFMTVKSNRELNR
                     AVAIGGIFILLMTGVAFTSGALSNVWFTKNEKGVKYCEISGAILEVCPGKKVVKIDEK
                     NI"
     gene            complement(1027..1197)
                     /locus_tag="PK348_03160"
     CDS             complement(1027..1197)
                     /locus_tag="PK348_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012631504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65244.1"
                     /translation="MLGLGDVWVFMAYLLCIASAILCAVYGFIKWNDDEEPYTDEAKR
                     WVQEEAEIEKTL"
     gene            complement(1581..2462)
                     /locus_tag="PK348_03165"
     CDS             complement(1581..2462)
                     /locus_tag="PK348_03165"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXK65245.1"
                     /translation="MYTITPTYFYSNNYKLDARLYLPDTGKKTPVFVMAHGFGARMDW
                     GLHPFADIFAQMGCAVLMFDYRGFGNSEGNNRRLVYHVHHIEDYHMAIQYARTIPNID
                     SSKVLLWGTSFSGGHVLTVASQDSSIAGVIAQVPFVDGVATAFHLPVKNILSGLWYGS
                     LDVVMSLITSKPVTIPLVATPDTFAAMNTPECYDGYMKLVPPEARDENWCPARVCLTL
                     PLYRPIAVVDKISCPVCIIGAQHDSLIPIKAVEKTAKRIKHVDFHILPCGHFDPYDGE
                     MFDKNIQIQKQFLQKVL"
     gene            complement(2536..4026)
                     /locus_tag="PK348_03170"
     CDS             complement(2536..4026)
                     /locus_tag="PK348_03170"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HXK65246.1"
                     /translation="MMKKVNNVFRINKITLQTMISVLYSILAILYCIPLSPYPVIAQE
                     NATIISYEQYISVITQKLPQIKNNVLQVEKAYNQVEKAKSVDDTNLIAKGQYARQKMY
                     TTGKSLYEPDYKQSYYGYMGIEQTLASTGTRVSLGLEYNASSITGNLTIPPQAYSVDE
                     YRPSITATITQPLLKNAFGIIDRYVKNSAAMKLEIQKLQQLQDDQSVLNYYKKLYFNW
                     VLYKQALDIWQESITNARSLVDSVKRKARAGLAENDDVQRAVSSLLNYQAQYKQYEIA
                     YKSLCDELSLYFDTSITPDINDMNSMFDNVVSAEYTEVPFEKTRSWLIVKKNMDVMQY
                     TINVADNSTLPQMNLILSASRKNYSDRQREALSKLPDTDYSVGVEFSYPLGNHDADAQ
                     LKDYELSLKELEHNLLITQNSYYKSLRTIMQYLNGYKELIATHNKNLQALISQRQTER
                     KKYEQARLDLQYLINTENSVAMEKLTLLQLKVGLLGYYIDYKDLTE"
     gene            complement(4007..4141)
                     /locus_tag="PK348_03175"
     CDS             complement(4007..4141)
                     /locus_tag="PK348_03175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65247.1"
                     /translation="MSEMRCADGSYDGYNEDTGKYSLTAGPLYYEFTEDEDEDDEESE
                     "
     gene            complement(4156..4833)
                     /locus_tag="PK348_03180"
     CDS             complement(4156..4833)
                     /locus_tag="PK348_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016548480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXK65248.1"
                     /translation="MLAIEASNVVKSFGDFTALKSISLNIRKGEFVAITGKSGSGKST
                     LFYSLSGLDVIDSGHVLVLGNNIHNLPQEDLHDFRNTKMGFVFQFHYLISELTGLENI
                     LLPAKKKNIHKEKREYALYLLDYFAIAHCKDKLPSQMSGGERQRVAVARSLIMNPEII
                     FADEPTGNLDSINSEIVMNIFREINLKNKTTVILITHDDEYAHSADREIRLKDGEIES
                     DVHYKKR"
BASE COUNT         1552 a         1054 c          829 g         1549 t
ORIGIN      
        1 tcaatattct tttcatcaat ttttacaacc ttcttacccg ggcaaacttc taaaattgcc
       61 ccactgattt cacagtattt gacgcctttt tcattctttg taaaccatac gttggaaagt
      121 gcaccagaag taaatgcaac gcccgtcata aggagtataa agattcctcc tattgctacc
      181 gctctgttca gctcacggtt actctttact gtcataaagc gtacaacaag ctgtggctgc
      241 gcaagcactc caatacccac acccattata attgttgaaa ccagcgtcca ccagaaatca
      301 gaacctaacg ctggcatgga tgtccatccc atatgtcctt ttgcagccag tggtggcgga
      361 ggtgtaagaa cagttaaatc agcatgtgat ttgataatac cacctaaatc gtagtatgta
      421 aagataataa gggttaacat accaatagcc attatggatc cctggaaggc atcggtatac
      481 attactcctt tcaggccacc cataataaca tatactgcta caattaatgt aaaaaacagt
      541 agcgctgtgg cataatctat tgcaaactgc tgttcaatgt attttgccgc accaatgaga
      601 actacagctg catacagagg cataaataaa aagattataa tccccgcaaa tttctgtaca
      661 aacggtgatt ggaaacgcct tcctaaaaac tcaggaaacg tatgtgcatc taaatgatac
      721 cccatacgtc gtgtacgttt tccaaagaat acaaatgcaa tgaatattcc aacaaaaata
      781 ttcaaaaaag taaggtacag taaccccata ccaaaaacac cagcagcacc tccaaatcca
      841 acgattgctg aagtactgat aaatgtagct ccatacgata gtgccatgat gaaaggatgt
      901 acccttcgtc ctgcaacaag atagtctgaa gcaccttttg tatggcggta tcccaaatac
      961 cctaaaaatg caacaattac aagatataca aatacccaga atatagtcat gacgcacctc
     1021 gttagtttaa agtgtttttt ctatttcggc ttcttcctgt acccagcgtt ttgcttcatc
     1081 ggtatatggc tcttcatcgt cattccattt tataaaacca tacactgcgc aaagaattgc
     1141 acttgcaata cacaacaggt aagccataaa aacccataca tcacctaatc ccaacatgca
     1201 aacctcctgc aataattatc taaaataatt ttaaaattac ctatgtacac cccactgctg
     1261 tagacagtga tataatagtg tacttttatg ctttgaatga atttataaaa aaaagtgaag
     1321 ctaattatag tataacaaca ttatatgtca agtgtttatt gctactatgt tatcatattt
     1381 ttaattttat ttagtatatt ctaattaaca tatttattgc atgcattata atactaataa
     1441 ttattattca caaaaaacac caaaaaaata atattcaggc aaaaaaagtc acactaatct
     1501 gaatgcaatg gcaattttac ttgcttattt tgcaaggttt agcgttaata cctgtatcct
     1561 tgagacaggt attaatattt ttacaaaact ttttgcaaaa attgcttctg tatctgtata
     1621 tttttatcaa acatctctcc atcatatggg tcaaagtgac cacagggtaa tatgtggaaa
     1681 tcaacgtgct taattctttt tgctgttttt tctaccgcct tgattggaat gagtgaatcg
     1741 tgctgtgccc caataataca taccggacat gaaattttat ccactactgc tattggcctg
     1801 tacaatggaa gagtaaggca aaccctggcg gggcaccaat tttcatcacg tgcttcaggt
     1861 ggtaccagtt tcatatagcc atcataacat tccggtgtat tcatagcagc aaatgtgtca
     1921 ggggtggcta ccagtggtat tgtaactggt tttgacgtta ttaaagacat cactacatcc
     1981 aggctaccat accacaggcc gcttaatata tttttaacag gtaaatgaaa agccgtggca
     2041 acaccatcaa caaaaggcac ctgagctata acccctgcta tagagctatc ctgtgatgct
     2101 acagtcaata catgccctcc gctgaatgat gtgccccata ataacacttt agagctgtca
     2161 atattaggaa ttgtcctggc atattgaata gccatatgat aatcttcaat atgatgaaca
     2221 tgatatacca gtcggcgatt attgccttca ctgttgccaa aaccacgata atcaaacatc
     2281 aacacggcac atcccatctg tgcaaagata tcagcaaatg gatgcaaacc ccaatccata
     2341 cgggcaccaa agccatgtgc cataacgaaa acaggagttt ttttaccggt atcgggaaga
     2401 tacagtcttg catctaattt ataattgttg ctgtaaaaat acgtgggtgt tatagtatac
     2461 atatgtgaac tcctacaaaa attaaatcaa aagagtttta attacagaat gcaaggaaaa
     2521 ttctttttac ttattttatt cagttaagtc tttatagtca atataataac caagcaaacc
     2581 taccttcagc tgcagtagtg taagcttttc cattgcaacg ctgttttcag tattaatcaa
     2641 atactgtaga tcaagccgtg cctgttcata cttctttcgt tctgtctgtc gttgtgaaat
     2701 cagagcctgt aaatttttat tatgtgttgc aataagctct ttatagccgt tcaaatattg
     2761 cataattgtc cgtaaagact tataataact attctgtgtt attaaaagat tatgttccaa
     2821 ttctttcagt gataactcat aatctttaag ctgcgcatct gcatcatggt tacccaatgg
     2881 ataactaaat tcaaccccaa ccgaatagtc tgtatcgggt aatttgctta aagcttcgcg
     2941 ctgccggtca ctataatttt ttcgtgatgc cgataaaata aggttcattt gcggaagagt
     3001 gctgttatca gcaacgttta ttgtatactg cataacatcc atgttcttct ttacaataag
     3061 ccatgaacgc gttttttcaa acggcacctc ggtatattct gctgaaacta cattatcaaa
     3121 catactattc atatcattaa tgtccggggt aatagacgta tcaaagtaaa gggataattc
     3181 atcacaaaga gatttatagg caatttcata ttgtttatac tgtgcctggt agttcaaaag
     3241 tgatgatacc gctcgctgaa catcatcatt ttcagcaagc ccggccctgg ctttgcgctt
     3301 tactgaatcc accagtgacc ttgcatttgt aatactttcc tgccatatat caagcgcctg
     3361 tttatacaaa acccagttga aataaagctt tttatagtaa ttcaataccg attgatcatc
     3421 ctgtaactgt tgaagtttct gtatttctaa tttcattgct gcactatttt tcacataccg
     3481 gtcaattata ccaaaagcat tttttaaaag aggttgtgtg atagttgccg ttatagaagg
     3541 tctgtattca tcaacggaat aagcctgtgg aggaattgtc agattacctg tgatagatga
     3601 agcattatat tcaagtccca aggacacacg ggttccggtt gaagctaatg tttgctcaat
     3661 gcccatatat ccgtaatacg attgtttgta atcaggttca taaaggcttt taccagttgt
     3721 gtacatcttt tgccgtgcat actgcccctt cgcaatcagg tttgtgtcat ccacactttt
     3781 agctttctcc acctgattgt aggctttttc cacctgaaga acgttatttt taatctgagg
     3841 caacttttga gtaattactg atatatattg ctcgtatgaa atgatagtag cattctcctg
     3901 tgcaatgaca ggatatggtg ataaaggaat acaatatagt atggcaagaa tgctatacaa
     3961 aacactaatc atcgtctgta gagtaatttt gtttatcctg aaaacattat tcactttctt
     4021 catcatcttc atcctcgtcc tcagtaaatt cataatacag tggtccagct gttaaggaat
     4081 atttcccagt atcctcatta tatccgtcgt atgaaccatc agcgcaacgc atttctgaca
     4141 aatcaccata cgcctctacc tttttttata atgcacatca gattcaattt caccatcctt
     4201 taaacgaatt tccctgtcgg ctgaatgtgc atactcatca tcatgggtaa tcagaatcac
     4261 cgtggtttta tttttcaaat ttatctcacg gaaaatattc attactattt cactgttgat
     4321 ggaatccaaa ttaccggtag gttcatcggc aaagattatt tcagggttca tgataagtga
     4381 tcgggcaaca gcaacacgct ggcgctcacc acctgacatc tgtgatggca atttatcctt
     4441 acaatgagct atcgcaaaat aatctaatag atacagagca tactcacgtt tttctttatg
     4501 gatgtttttc tttttagcag gcaaaaggat attttcaagc cccgtcagct ctgagatcag
     4561 ataatgaaac tgaaacacaa aacccatttt tgtgtttctg aaatcatgaa ggtcctcctg
     4621 gggcaggttg tgaatattat ttccaagaac cagcacatgc ccgctgtcaa tgacatcaag
     4681 gccactgagc gaataaaaca acgtagattt gcctgagcct gattttcccg ttatggctac
     4741 aaattcacct ttccgtatat tcaggctaat tgactttaat gcagtaaaat cgccaaaaga
     4801 cttcaccacg ttggaagctt ctattgccag cattattccg ttctcctgat aatttcaata
     4861 ggcgatagtt tgccagcatt ctttgccggt aaaaagcttg caagcagcgc agaagttacc
     4921 accagcataa aaccccacat gtatattgtt atatcccagg caaccatcat tggccttcca
     4981 ggaa
//
LOCUS       DAOSZP010000097         4974 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_14074_length_4974_cov_3.015654, whole genome shotgun sequence.
ACCESSION   DAOSZP010000097 DAOSZP010000000
VERSION     DAOSZP010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4974)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4974)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4974
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14074_length_4974_cov_3.015654"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            214..504
                     /locus_tag="PK348_03185"
     CDS             214..504
                     /locus_tag="PK348_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015403942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HXK65249.1"
                     /translation="MIFSEEQVGFTSELLKSIAHPIRLKVLCFLMDGEKTVGEIEKEF
                     GSTISNISQHLTILRKMNIIHRRKEANFMYYSIKDERILKLISTLKNLYCAQ"
     gene            527..2089
                     /locus_tag="PK348_03190"
     CDS             527..2089
                     /locus_tag="PK348_03190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65250.1"
                     /translation="MILFLYSDVFSLPKDITIQGTVIYNQDYLVRAADIDTIPASKFT
                     FGTIAAAITKFYHDNGYTLARVLLVRETLASIAVFVDEGRLNRIVFKNLNSLDTLRIQ
                     YDFSLPNRIYHTQTVDKQLEKLKKKYAIKDFIVTLQESKDYSSALFQIDRLSKPFTDG
                     KLPLVPTIPAVPDPHYDLIITIVAFSPEESGGFTYGLDTSFSLGLIPYVRYTHNDLIQ
                     RGDSFRIGFKTGFMYGLDGNFTSLPYNTFNQIKSTYNFRSHFDGLFIPRVSSEVYNSK
                     AGRKDLGLDQYRYVRVNVLAEPGFTPIEKLKVYPGYGVEKVFILQAVKNPDTAVTQPN
                     IAKDTQVWHFIGIKANIDLIPFSLKHTTGRKITLEAYRYQNATHFYKVGIDTIVKFEF
                     FNFDLFVFQGDYTYLNDPVPFYYEEPVSTRTFKGFMGKDYHTTHIARASFEYEISIHR
                     DFIYTGVYNDFTAFKGKYLLHGNQIGIAYGLSFHYVFFEQFEFALWWGKDYLPSTDQS
                     GYNLKFTLAKKW"
     gene            complement(2340..2474)
                     /locus_tag="PK348_03195"
     CDS             complement(2340..2474)
                     /locus_tag="PK348_03195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65251.1"
                     /translation="MIKENKSSLCVAVAHTVIHTTNTKKEYPVIIDRGGMQVKVNEGG
                     "
     gene            complement(2538..3032)
                     /locus_tag="PK348_03200"
     CDS             complement(2538..3032)
                     /locus_tag="PK348_03200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1640 domain-containing protein"
                     /protein_id="HXK65252.1"
                     /translation="MGFAMRVYEAFKDDEVKAKVLAEFIEKVEEAINNNQVATRQDLH
                     VQELKLTKEIEQTRLEIKEAELKLTKEIEQVRADMKETELKLTKEIEQVRVDLTKDIE
                     KMRLEIKEIELKLTKEIERVRAELKIEIQQTKVSLLKWLIGLLLTQTITIIGVIIGLF
                     QVIK"
     gene            complement(3548..4354)
                     /locus_tag="PK348_03205"
     CDS             complement(3548..4354)
                     /locus_tag="PK348_03205"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HXK65253.1"
                     /translation="MEFATLLHDTVFCALDCETTGLNPIIDRIVSIGIIRFTLDRVID
                     TFYTLVNPLKPIPPHVTRLHGITADMVQKAPVITELFDTIEQYTSNTYCVIHNPRFDI
                     SFIDSAYHQHDRNTPDLKSFDTVHLARKTFKKLPSYNLGNLCKFFGISINAHHALSDA
                     QGSMEIFKKVIDKLNAHNWCIRDLERYHGGIISAYSLGIKSTHIPADSRIPLGYRITI
                     RYKDAQGNIVERSILPQRYFIMGKNNYIQAYCYLRNEIRYFNTARIVAVY"
     gene            complement(4360..>4974)
                     /gene="murB"
                     /locus_tag="PK348_03210"
     CDS             complement(4360..>4974)
                     /gene="murB"
                     /locus_tag="PK348_03210"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:TIGR00179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HXK65254.1"
                     /translation="VVVDAKVTKKRFVEEAIEHGLAGMEFMAGIPGCIGGGVIMNAGT
                     TMGTFSDIITDITCYTADGTLLTVANTSLFSYRKSMLPEGAIVISARCTLNIADNPED
                     VKNTVKEILRERKNKHPLDYPSAGSVFKNPDGHASWKLINDAGLRGYSIGGAKVSELH
                     TNFIVNTGKATAMDIKHLVEFIQETVHKLYDINLEPEIRFIGEF"
BASE COUNT         1447 a         1009 c          852 g         1666 t
ORIGIN      
        1 atagagacct aagattaaga taaagtaaaa aaatattgac aacatcaccg atattgctac
       61 cttgttatta aacagtaaaa taattgttta attaaaatct taattacttg ttgttttaaa
      121 acttttttta catttgcatt ttttcttttg tatagcacta tataatgagt taacaggatt
      181 gtattataca gcacagaggg ggactttata tatatgatct tttctgaaga acaggtgggg
      241 tttaccagcg aactactaaa atccattgcc catcccatac ggcttaaagt attgtgtttt
      301 ttaatggatg gtgaaaaaac ggtaggcgaa attgaaaagg aatttggctc caccatatca
      361 aatatatccc agcaccttac cattttaaga aaaatgaata ttatacacag acgcaaagaa
      421 gccaatttta tgtattactc tattaaagat gaacgcatat taaagcttat ctccacatta
      481 aaaaatttgt actgtgcgca ataaatgcat tttactttta ccagtattga ttctgttcct
      541 gtattcggat gtttttagcc ttcctaaaga cattaccata cagggcactg ttatctacaa
      601 tcaggactac ctggtccgtg ctgccgacat tgataccata cctgccagta agtttacttt
      661 tggaactatc gccgccgcaa taactaaatt ttatcatgac aatgggtata cgctggcacg
      721 tgttcttctg gtgcgcgaaa cattagcttc cattgccgta tttgttgatg agggacgctt
      781 aaatcggatt gtgtttaaaa atttaaacag ccttgacacg ctgcgtattc agtatgactt
      841 tagcctgcca aaccgcattt accatacaca aaccgttgat aagcaattag aaaagctcaa
      901 gaaaaaatat gctattaaag actttattgt gacattacag gaatcaaaag attattccag
      961 tgcccttttt cagatagaca ggttgagcaa gcctttcact gacggcaaac tgccgctggt
     1021 accaacaatt cctgcagttc ctgatccaca ctatgattta attattacca ttgtagcatt
     1081 ttcaccagaa gagtccggtg ggtttaccta tggccttgat accagtttta gtttaggact
     1141 cataccgtat gtacggtata cccataatga tctcatacag cggggcgata gtttccggat
     1201 aggttttaaa actggtttta tgtacgggct ggatggaaat tttacttcac ttccctacaa
     1261 cacgtttaat cagataaaat ccacctataa cttcagatca cactttgatg gcctgtttat
     1321 ccccagggtt tcatctgaag tgtacaattc aaaagccggg cgaaaggact tagggcttga
     1381 ccagtatagg tatgtaaggg taaacgttct ggccgaacca ggatttaccc ccattgagaa
     1441 attgaaagta tatccgggat atggcgttga aaaagttttt attttgcagg ctgttaaaaa
     1501 ccctgatact gcagtaacac agcccaacat agcaaaagat acgcaggtgt ggcactttat
     1561 tggcattaaa gcaaacattg accttattcc attttcgcta aaacatacaa cagggcgtaa
     1621 gataaccctt gaagcctacc gctatcaaaa tgcaacccat ttttacaaag tgggcattga
     1681 tactattgtt aaatttgaat tttttaactt tgatttgttt gttttccagg gtgattatac
     1741 ctatcttaat gacccggtgc ctttttatta tgaggaacct gtttctacac ggacgttcaa
     1801 aggattcatg ggcaaggatt accatacaac gcatattgca cgtgcttctt ttgaatacga
     1861 aatttctata cacagggatt ttatatatac cggtgtgtac aatgacttta ctgcttttaa
     1921 aggcaaatat cttttacatg gtaatcagat aggcattgct tacggcctga gttttcatta
     1981 cgtatttttt gaacaatttg aatttgcact atggtggggc aaggattatc ttccttccac
     2041 tgaccagtcg gggtacaacc ttaaatttac attggccaag aaatggtagg tggtatttca
     2101 tgcgtgtttg ctggatgcat attgggtact ggctaaaaaa tggcaagggc tttttcatgc
     2161 gtgtttgctg cacgcatatg catagtttca cagtaaccgt caaactaacc gcaggttaga
     2221 tgaatctata tcaattttaa aaacgcaaga aaaatttatt ttcgtacttt actttcaggt
     2281 aagcatatat cattcaacga ataatattaa gatacatagc acttatgcta atgtatccct
     2341 taaccaccct catttacttt tacctgcatg ccacctctgt ctattatcac gggatattcc
     2401 tttttggtat ttgttgtatg tataacggtg tgagctactg caacacatag actgctttta
     2461 ttttctttaa tcatcaggca actgcccata ccaccgggca aattggatat aattcagatt
     2521 tatatcataa attactatta ttttatcacc tgaaacaaac caataatgac tcctattatg
     2581 gttattgttt gcgttagcaa caaccctata agccatttta acaggcttac ctttgtttgc
     2641 tgtatttcaa tttttaactc ggctcgcacc cgttctatct cttttgttaa cttaagctct
     2701 atctctttta tttccagacg cattttctct atatcctttg taaggtcaac acgcacctgt
     2761 tctatctctt ttgttagctt cagctctgtc tctttcatat cagctcgcac ctgttctatc
     2821 tcttttgtta atttcagctc tgcctctttt atttccagac gcgtttgctc tatctctttt
     2881 gttaacttta attcctggac atgtaaatcc tgccttgttg ccacctgatt gttatttatt
     2941 gcttcctcta ccttttcaat aaattcggcc aagacttttg cctttacttc atcatcctta
     3001 aaggcttcat atacccgcat tgcaaatccc attgtactat acccttcctt atgtattttt
     3061 tctacgaatc atactctatt cattctacag actttcttgt tatcagtttt ttcccataca
     3121 gtaaatataa tctacatgca atacctgcgt caataaaatt tatttttttc ataattcctg
     3181 gcattgcgga tataataata ctcattgccc atttcatgat taaaatacat gaaatctctt
     3241 tattaatttt ctgcacttat ttaaaaatta ttttaaattt ttcccaactc ctgcacaatt
     3301 acatctaaaa ttcataattc aattttctga ataattcaat gggtataatt catatatgga
     3361 tatatatatt tatagggttg gctaaggctc ccgaagccac ccctgtttta tattacggtc
     3421 gtttcgacag gctcagcaca ggcgtttcgt taggctcagt gaccggtcat cgagtagcac
     3481 tacaggaatg tatcgagaca accgcattgt attataatga atatatttca taaccagatg
     3541 gtaaggttta atacaccgct actatacgag ctgtattgaa ataacggatt tcatttcgca
     3601 gataacagta tgcctgtatg tagttgtttt ttcccattat aaagtagcgc tgtggcagta
     3661 tgctgcgctc cacaatattc ccctgtgcat ctttatatct gatagttata cggtacccca
     3721 agggtatcct gctatcggca ggaatatgtg tgctttttat gccaagtgaa taggcactga
     3781 taataccacc atgatagcgt tccagatcac gaatacacca gttgtgcgca ttgagcttat
     3841 caatgacctt tttaaatatt tccattgatc cctgtgcatc gctcagtgcg tggtgagcat
     3901 taatggatat accaaaaaat ttacaaagat tgcctaaatt atagcttgga agtttcttaa
     3961 atgtcttgcg tgctaaatgt actgtatcaa atgacttcaa atctggagta tttctgtcat
     4021 gctgatggta tgcagaatca atgaatgata tatcaaaacg tggattgtgg ataacacagt
     4081 aggtattgct ggtatattgt tctatagtat caaataattc tgtaataacc ggagctttct
     4141 gtaccatgtc agcagtaatt ccatgaagcc gtgtaacatg tggtggaatt ggcttcaatg
     4201 ggtttaccag tgtatagaat gtgtcaatga ctctgtctaa tgtaaaccgt attattccaa
     4261 tagaaactat cctgtcaatg atgggattta atccggtagt ttcacaatcc agtgcacaga
     4321 atacggtatc atgtaatagt gttgcaaatt ccatgtatat taaaattccc ctataaaacg
     4381 tatttcaggc tctaaattga tatcatacag tttatgcacc gtttcctgaa taaattcaac
     4441 cagatgcttg atatccatag cagtggcctt gccagtattt acaataaaat ttgtatgtaa
     4501 ctctgacact ttggcaccgc caatactgta cccgcgtaaa cccgcatcgt tgataagctt
     4561 ccatgaagca tgcccgtcag gatttttaaa gactgatcca gccgaaggat aatccagcgg
     4621 atgcttgttt ttgcgctcac gcaaaatctc ttttacagta ttttttacat cctccgggtt
     4681 atctgcaata tttaatgtgc accgggcact tataacaatg gcaccttccg gcaacatgga
     4741 ttttcggtat gaaaataacg acgtgtttgc aacagttaac agcgtgccat ctgcggtata
     4801 acaggttata tcggtaataa tatctgaaaa agtccccata gtggtgccag cattcatgat
     4861 gacaccacca ccaatacagc ctggtatccc tgccataaac tccataccag caagcccatg
     4921 ctctattgct tcttccacaa atcgtttttt tgttaccttg gcatcaacca caac
//
LOCUS       DAOSZP010000098         4968 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_14092_length_4968_cov_3.602483, whole genome shotgun sequence.
ACCESSION   DAOSZP010000098 DAOSZP010000000
VERSION     DAOSZP010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4968
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14092_length_4968_cov_3.602483"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1012
                     /locus_tag="PK348_03215"
     CDS             <1..1012
                     /locus_tag="PK348_03215"
                     /inference="COORDINATES: protein motif:HMM:NF014801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HXK65255.1"
                     /translation="IYDTRKGRLGWHKGLCKIFDVNMEHLPPVFNSTDTIGTITKKAA
                     RELGIAEGIPVFGGGGDVPLTLVGSGCTDLYDTNIYVGTSGWVSSNVDKRMVDIGNFI
                     ASILGAIPNHYVYVAEQETAGACLKWVRDHLALDEIGMYLQARHIADKSREYESLYDF
                     LNTVVMQTPPGAGNVLFTPWLHGNRSPREDPYARGMFFNLSLTTGKRQMIRSVLEGVA
                     YHILWMLEAMEKKIPRQEVLRFAGGGAKSDVWCQIMADITGRNIEAIENEINAGTVGA
                     SVVCAVGLGILSSFNEAKRLVGVRKIFKPQPQYTDMYTRNFKVFTKLYEKNKDLFKIL
                     NY"
     gene            1030..2487
                     /locus_tag="PK348_03220"
     CDS             1030..2487
                     /locus_tag="PK348_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008595555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HXK65256.1"
                     /translation="MKQEYAISHWPDSKEILEKLRVLANELPVYQINRNKMNEYLEKY
                     ETKFKSSKQLVEKARQRIPGGVQHNLAFNYPFPLAIAKADGAYLYDVDGNKFIDFLQA
                     GGPTVLGSNYKPVRDKVIEVINECSPVTGLFHEYELLLAELIHSHIPSVELFRSLASG
                     TEGCMAAIRAARCFTDKKYIIKVGGAYHGWSDQMVFAMRIPGLGTLDSHGIPEGCYTY
                     MQEFYPNDIEGLEKLLKENESKGGTAAVIVEPLGPESGTRPVHYDFNAKVRELCDKYN
                     SLLIFDEVVTAFRVGLGGAQGYFGVKPDLTVFGKCVAGGYPAAGGVGGRREIMETFAA
                     GIQGLKKRAMVGGTLSANPLSCAAGYFAIKAIEETQAHIKAGKAGDKLTQGLQEIFKR
                     YDLPFVAFNHGSICHMETGAAMLMELTNPKIFEQVGQRRKVMEELGAALMVEGIITLA
                     GSRIYTSMADTDDVIDDALERFDRVFKYFEPVKEK"
     gene            2489..3700
                     /locus_tag="PK348_03225"
     CDS             2489..3700
                     /locus_tag="PK348_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016549415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXK65257.1"
                     /translation="MTHEIKVYNYRWIILFIFALLNIVIQIHWISFASITSEAAAFYN
                     VTPLSIGFLSMLFMIVYVFISLPASYIIDTHGIKLGIGIGAVLIGIFGIIKGLYAYSF
                     NAIVTTQIALAIAQPFILNAYTRLSAKWFPLDERATAAGIASLAQYIGIIVGLAATPF
                     LVHSYSIPHTMYIYGILSIAIAVLFLLFMKEEPPTPPCEKGQEERFKVKEGLQHIFSM
                     KQMWLVILLFFIGLGMFNAVTTWIEQILEPRGFNSEQAGIVGASMMAGGILGAIILPL
                     LSDHYRKRKVFLTICMAFLIPGLAGLTFAQHFNTILISSFILGFFIMSAGPIGFQYAA
                     EISYPAPESSSQGLLLLSGQISGILFIFAMDAFRTSTGAMTPFMIAFIVLMVINTLLT
                     FKLKESNLISS"
     gene            3838..4491
                     /locus_tag="PK348_03230"
     CDS             3838..4491
                     /locus_tag="PK348_03230"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HXK65258.1"
                     /translation="MSQYTMYKEQILQWCQWLNEHGFFGTQLGSGGNISCRIENEEKI
                     AVTPSSLPYKDMTTSDICIVDYNQNVIEGKKPTMELSMHLAIYKARDDISAVVHTHQI
                     YASILSVMNIDKPISAMFDEIAFAIGDEVAFVPYALSGTPELASNVASAVTNKCKCYI
                     IKNHGAIALGKNLEQACLHAEMLEKVSMVYYYSLAAGKEAGSLPEPIMQVIRQLFGK"
     gene            4621..>4968
                     /locus_tag="PK348_03235"
     CDS             4621..>4968
                     /locus_tag="PK348_03235"
                     /inference="COORDINATES: protein motif:HMM:NF022666.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3015 family protein"
                     /protein_id="HXK65259.1"
                     /translation="MKKSVSLLVLFMLVAAISIAEAGVVRNNAGCGVGSMIFGDKDGL
                     LFEILATTTNGICGNQTFGMTSGTLGCAPMKGIVSNEKINLYVADNMDNLAKDIAKGN
                     GEYLETLALLMNVP"
BASE COUNT         1499 a          883 c         1081 g         1505 t
ORIGIN      
        1 tatttatgat acacgaaaag gaagactggg ctggcataaa ggcttatgca aaatatttga
       61 tgtaaacatg gaacacctgc cccctgtgtt caactcaaca gatactatcg gtactattac
      121 aaaaaaagct gccagggaat taggtattgc tgaaggtatt ccagtctttg gcggtggtgg
      181 tgatgttcca cttactctgg ttggttctgg ttgcactgac ctgtatgata caaacatcta
      241 tgttggaacc agcggatggg tttcatctaa cgttgataaa cgtatggttg atattggcaa
      301 ctttattgct tcaatcttgg gagctattcc caatcattat gtgtatgttg ccgaacagga
      361 aactgcgggt gcctgtttaa aatgggttcg tgatcattta gctttagatg aaatagggat
      421 gtatttgcag gctcgccata ttgcagacaa aagtagggaa tacgaatctt tgtatgattt
      481 tttgaatacg gttgtaatgc agacacctcc cggtgcggga aatgtacttt ttacaccatg
      541 gctgcacggc aaccgttcac cacgtgaaga cccgtatgca cgtggtatgt tctttaacct
      601 gagtcttaca actggtaagc ggcagatgat acgcagtgtg cttgaaggcg ttgcatacca
      661 catactctgg atgctggaag cgatggaaaa aaagattccc cgtcaggaag tgctgcgatt
      721 tgccggtggt ggtgcaaaat ctgatgtatg gtgccagatt atggccgata ttaccggcag
      781 aaatattgaa gctattgaaa atgaaatcaa cgccggtact gttggcgctt cggttgtttg
      841 tgctgttgga cttggaattt tatcttcatt taatgaagca aagcgcctgg tgggagtgcg
      901 aaaaatattt aagccacaac cacaatacac agatatgtat actcgcaatt ttaaagtttt
      961 taccaaactg tatgaaaaga acaaagatct ttttaaaata cttaattatt agagttcagg
     1021 aggaatacaa tgaaacagga atatgccatt tcacactggc cagattcaaa agaaattctt
     1081 gaaaaattga gggttcttgc aaatgaacta ccggtgtatc agattaaccg taataaaatg
     1141 aatgaatacc ttgaaaagta tgaaacaaaa tttaaatcat caaagcaatt ggtagaaaaa
     1201 gccaggcaac gaatacctgg tggtgtacag cataacttag cttttaatta tccatttcct
     1261 ttagcaatag caaaagctga tggtgcctac ctatatgatg ttgatggcaa taagtttata
     1321 gattttcttc aggctggtgg cccaactgta cttggcagca attacaaacc tgttcgtgat
     1381 aaagttatag aagtgatcaa tgagtgcagc ccggttacag gcttatttca tgaatatgag
     1441 ttacttctgg cagagcttat acactcacat attccatctg ttgaattatt ccgatctctt
     1501 gccagtggaa ccgaaggatg tatggcagca ataagggctg cacgatgctt caccgataaa
     1561 aagtatatca tcaaggttgg cggtgcctat cacggctgga gcgatcagat ggtatttgca
     1621 atgcgaatac cgggactggg aactctggat tctcatggca taccagaagg ttgctatacc
     1681 tacatgcaag aattttatcc aaatgatatt gaaggcttgg aaaaactact gaaagaaaac
     1741 gaatcaaagg gtggcactgc tgctgttata gtagagccgc tgggtcctga aagcggtaca
     1801 cggccagtac attatgattt taatgctaaa gtgcgtgaac tatgcgataa atacaattca
     1861 ctgctcatct ttgacgaggt ggttaccgct tttagagttg gacttggcgg tgcgcagggc
     1921 tattttggtg ttaaacccga tttaacagta tttggcaaat gtgttgcagg tgggtatcct
     1981 gctgcaggag gtgttggcgg cagaagagaa attatggaaa cgtttgcagc aggtattcag
     2041 ggcttaaaaa agcgcgccat ggttggtggc accctatctg caaatccttt gtcatgcgca
     2101 gctggatatt ttgccattaa agccattgag gaaacacagg cacacatcaa agcaggaaaa
     2161 gcaggtgata agcttacaca aggattacag gaaatattta aacgatatga cctacccttt
     2221 gtagcattta atcatggctc aatatgccac atggaaactg gtgctgccat gttaatggaa
     2281 ttaaccaacc ccaagatatt tgagcaggtt ggacagcgtc gtaaggttat ggaagagctt
     2341 ggggcagcac ttatggttga aggcatcata acacttgcag gaagccgcat ttacacaagt
     2401 atggctgaca ctgatgatgt cattgatgat gcattagagc gatttgacag agtatttaaa
     2461 tattttgaac cagttaagga gaaatgatat gactcatgaa ataaaagtat acaattaccg
     2521 ctggataatt ttgtttattt ttgcattact gaatattgtt attcagattc actggatatc
     2581 ctttgccagc attacttcag aagcagcggc gttttacaat gtaacgccgc tgtcaattgg
     2641 ctttttatca atgctcttta tgattgtcta tgtttttata tcactacctg cttcatatat
     2701 tatagacacg catgggatca agcttgggat tggcattggt gccgtactca ttggtatatt
     2761 tggcattata aagggtttgt atgcatattc ttttaatgcg atagttacta ctcaaatagc
     2821 gttagccata gctcagccat ttatcctcaa tgcatataca cggctatctg caaaatggtt
     2881 tcctttagat gagcgtgcga cagcagccgg catcgcatcg cttgcacaat atattggcat
     2941 aatagttggg cttgctgcta cgccgtttct ggtacattca tattctatac cacacacaat
     3001 gtatatctat ggcatcttaa gtattgccat tgcagtacta tttcttcttt ttatgaaaga
     3061 agagcctcct acaccaccgt gcgaaaaagg gcaggaagaa cgcttcaaag taaaagaggg
     3121 gctacagcat atattcagca tgaaacagat gtggctggta atacttcttt tctttatagg
     3181 acttggaatg tttaacgcag ttacaacatg gatagagcag atacttgagc cacgtggctt
     3241 taattctgag caagccggta ttgttggtgc cagcatgatg gctgggggta ttcttggagc
     3301 aattattctg ccgttacttt cggaccatta tagaaagcgc aaagtttttt taacaatctg
     3361 tatggcattt cttattccgg gacttgcagg tttaacattt gcacaacatt ttaatacaat
     3421 tctgatttca agttttattc ttggtttttt tatcatgagt gcaggcccaa ttggattcca
     3481 gtatgcagca gagatttcct atcctgcacc cgaatcaagt tcacagggcc ttttactctt
     3541 atctggtcag atttcaggaa tactctttat ttttgccatg gatgcattca gaacaagcac
     3601 tggtgcaatg accccattta tgattgcctt tattgtgctg atggttatta atacactatt
     3661 gacatttaaa ttaaaagaat cgaatttaat cagttcataa tattttcaat gtgataatga
     3721 cctaattttt aacataacga agagagtgga atcatattcg taaagcaaag attattacac
     3781 tatggtaatt caaaataaat accatgaata tattttatat ctttatagga gttaaccatg
     3841 tcacagtaca caatgtacaa agaacaaatt ttacaatggt gccaatggct caatgaacac
     3901 ggattttttg gtacacaatt gggcagtggt ggaaatatat catgcagaat tgaaaatgaa
     3961 gaaaaaattg cagtaactcc atcaagtctg ccctataagg atatgaccac gagcgacatt
     4021 tgcattgttg attataacca gaatgttatt gaaggcaaaa agcccacaat ggaattgtca
     4081 atgcatcttg caatttataa agcaagggat gatatcagtg cagtagtaca cactcatcag
     4141 atttatgcaa gcatactgtc ggtcatgaat attgataagc ccatctcagc catgttcgat
     4201 gagattgcat ttgcaattgg tgatgaggtt gcttttgttc cctatgcact atcgggcaca
     4261 cccgagcttg ccagtaatgt tgcttctgct gttaccaata aatgtaaatg ttatatcatt
     4321 aaaaatcatg gtgctattgc tttaggcaaa aatttagagc aagcctgcct tcatgctgag
     4381 atgctggaaa aggtttccat ggtgtattat tattcactgg ccgcagggaa ggaagctggc
     4441 tcactccccg aacccataat gcaggtaata cgccagttat ttggtaaatg atggttgaag
     4501 ttcaaagttt cggtttaata gtaacaaaat ttttgcttga cattatatga aatatttata
     4561 gtatttttat aaaatataaa aatatcccct atactatata ctattttacg gaggtttttt
     4621 atgaaaaaaa gcgtatcatt acttgttctt tttatgctgg tagcagcaat aagcatagct
     4681 gaagcaggtg ttgtacgaaa caatgcaggt tgtggcgttg gctcaatgat ttttggcgac
     4741 aaagatggat tgttatttga gatattggca acaacaacaa atggaatttg tggcaaccag
     4801 acatttggca tgacttcagg gacactgggt tgtgctccta tgaaagggat tgtttcaaac
     4861 gaaaagatca acctttatgt agcagataac atggataacc ttgcaaaaga cattgctaaa
     4921 ggcaatggtg aatatctgga aacattagct cttcttatga atgttccc
//
LOCUS       DAOSZP010000099         4943 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_14176_length_4943_cov_3.218290, whole genome shotgun sequence.
ACCESSION   DAOSZP010000099 DAOSZP010000000
VERSION     DAOSZP010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4943)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4943)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4943
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14176_length_4943_cov_3.218290"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1313)
                     /gene="glpA"
                     /locus_tag="PK348_03240"
     CDS             complement(<1..1313)
                     /gene="glpA"
                     /locus_tag="PK348_03240"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012174449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046174 - polyol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HXK65260.1"
                     /translation="MKTLETDVIIIGGGATGCGVLRDCTLRGINAVLIEKNDIASGTT
                     GRNHGLLHSGARYAVKDLESATECINENRILKKIAWHCIEPTGGLFVTLPEDDPTYHN
                     RLLESCKFAGIDAKEISIKKALAIEPNLNPNILSAIWVPDGTIDPFRLASANILDSVE
                     RGAIVFTHTEVERIMLKQNQVAGVQCYDKVTKEYFEVYAPIIVNASGVWGQHLCSTAG
                     IELKMFPSKGSMVIIDYRINNVVVNRCRVPSDGDIIVPGDTVSLIGTTSRKVSYDKID
                     ELVVDDDEIDVLLADGEKLIPNVSKARVLRAYCGVRPLVAVSGEMDGREISRGVVLID
                     HKERDNVQGLITIAGGKLMTYRLMAEMTTDLVAKKIGNTKSCVTHKIPLPGSEKRVGY
                     TKKIKYFSGIPNSVVGSTHYRHGERVHNILSLQKRNYGLICECEMV"
     gene            complement(1584..2204)
                     /locus_tag="PK348_03245"
     CDS             complement(1584..2204)
                     /locus_tag="PK348_03245"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HXK65261.1"
                     /translation="MKAELRRQHILDCAKKVFAEKGYYEAYVEDVIKVAHIGKGTIYE
                     YFRNKEDLFLSLLIRSLEEWKRTVSISHQEIKTLDPVSYFRHRIKLTIIFLNNDRELT
                     SILLKTGFGFNPHIQKLIGEFEKNLRKHILHDLRLAKHLGVLRDGVDLQITTSILIGG
                     LLMIAYQAILSVPRLSEPEIDELTDKFMVTFIRGMFKPEFVNQYIN"
     gene            complement(2291..>4943)
                     /locus_tag="PK348_03250"
     CDS             complement(2291..>4943)
                     /locus_tag="PK348_03250"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HXK65262.1"
                     /translation="GIASLIASLTAGSYRIILGGPGVYVGVASVIVSALSGLVVRRIF
                     KNEPTNIPLYGIVLFAVITHVLVLLCQLLLPWPRGITVIATIGIQYLIVLSSGLCAIA
                     LIFKTIENRTLALQNLQASADLLHDVLSVNPSVVFSMDPSTKTITWISPNVEVVLGYK
                     QDEIINTPLNSYIHKDDVYLYDKMFEEPGDNNFISVECRIITLYQTVVWGDIKIRVIR
                     DEKGKIAALFGSISDITQRKKVEHELKESEERYRTIFKNSKAVMLIIDPDTGDIVDAN
                     EAAVEFYGWSRKELQSMQISQINTLTPEEIRARMEEARACKRVYFEFKHRRKDGSIRD
                     VDVYSSAITIKGKQYLFSIIHDVTAKRQAEEALKKNEELFRLIFQNAPMGIYAFDENG
                     VITACNDNFVKIIGSSREALIGLNTLKLPDERVVDAVNKCLQGIQSEYNGWYKSVTAD
                     KITPARALFSPLIVENKIVGGLAIVEDMTYQLEAEAQKEKLEEQLRQAQKLEAIGRLV
                     GGIAHDFNNILSIISGYAELIKIKIEKDNPIYSDLEEISNATKRSAEIVRQLLTFSRQ
                     QKVNPIRVNPNYIIRSTEKMLAAMVGENIKVQLELADDVYDIWIDTATFVQILTNLAS
                     NSRDAIKDVGNIKIVTKNRYLDEEFCTIHPGCKPGDYVMIQFIDDGSGMDKETLERLF
                     EPFFTTKEVGKGTGLGLSTVYGIVKQFNGFINVYSEQDKGTVVNIFFPKYVEQLPEVQ
                     LSGHVRDNGRTEDRGIGISKNKILVVEDDLIILDMLKKMLLLLDQDVDAFSDPLEMLQ
                     VAENYKNNIDILITDIVMPQMNGIELFNRLKLIIPDMKVIFISGYSDDSYRDETKGIK
                     HSVFLQKPFTLDELKNALLKLDQNYEQ"
BASE COUNT         1514 a         1044 c          729 g         1656 t
ORIGIN      
        1 accatttcac attcgcagat aagcccataa ttacgcttct gcaaactcag gatattgtga
       61 acacgttcac cgtggcggta atgcgtggag ccaactactg aattgggaat acctgaaaaa
      121 tattttattt tctttgtata gccaactctt ttctctgaac cgggaagggg tattttatgg
      181 gtaacacagc ttttagtatt accaatcttt tttgccacaa gatctgttgt catttctgcc
      241 atgaggcgat acgtcataag tttgccacca gctatggtaa tgagaccctg aacattatca
      301 cgttctttat ggtcaatgag cacaacacca cggcttattt ctctgccatc catctcacca
      361 gaaactgcaa caagcggcct taccccacaa tatgctcgca acactcgtgc cttggaaacg
      421 ttgggtatta atttttcgcc atcagctaat agcacgtcta tctcatcatc atcaacaaca
      481 agttcatcaa ttttgtcata ggacactttt ctggaagttg taccaattaa tgataccgta
      541 tcaccaggaa ctattatatc accatccgaa ggcacacggc accggtttac cacaacattg
      601 ttaattctgt aatcaataat aaccatggaa cccttggaag ggaacatctt cagttcaata
      661 cctgcagtag aacataaatg ttgaccccac acaccagatg catttactat aattggtgca
      721 tacacctcaa aatattcttt agttacctta tcataacact gtacacccgc tacctgattt
      781 tgtttaagca tgatacgttc cacttctgta tgggtaaaaa ctattgcacc cctctccact
      841 gaatccaaaa tatttgcaga agctagcctg aatgggtcta tggttccatc aggcacccat
      901 attgcagata gtatattggg atttaagttt ggttctattg ccagagcttt ctttatactt
      961 atttctttag catcaatacc agcaaattta cacgattcaa gcagcctgtt atgataggtt
     1021 gggtcatctt caggaagagt tacaaacaaa ccacctgttg gctcaataca atgccatgct
     1081 atttttttta atattcggtt ttcattaata cactcagttg ctgattcaag atctttaaca
     1141 gcataacggg cacctgaatg taaaaggcca tgatttcgac ctgttgtacc tgaggctata
     1201 tcattttttt caattaaaac tgcatttata ccgcggagag tgcaatcacg caacacaccg
     1261 catcccgtag ccccgccccc aattattatt acatcagttt ctaatgtctt cacgtctctt
     1321 ccttttaaaa ttatagcaaa tcaactataa ttgcactata cctgatttca aaattgtata
     1381 gatcccgata attcttataa tagaatactg tataatactg ctttttagga attatcattt
     1441 gaaacctgat atcttcaatc actgaatggc attaatgtaa taacgacctg catatgtagt
     1501 gataaaatat atggaattaa ttattgcgca tgttgcaggt aaatcataaa aatttaagat
     1561 atacttctta taaaaaaata ttgtcaatta atatattgat ttacaaattc aggtttaaac
     1621 atgccccgta taaatgttac cataaactta tcggttaatt catcaatctc aggttcagat
     1681 aaacgaggaa cagacaaaat tgcctgatag gctatcatta aaagaccacc aatcagtata
     1741 cttgttgtta tctgcaaatc cacgccatct cgtaaaacac ccagatgttt tgcaagccgt
     1801 aaatcatgca atatgtgctt gcgtaaattc ttttcaaatt cacctataag tttctgaata
     1861 tgtggattaa aaccaaaacc agttttcaac aggattgaag taagttcccg atcgttattc
     1921 aggaatataa ttgttaactt aatcctatgt cgaaaataag atactggatc taatgtttta
     1981 atttcctgat ggctgatact gacagtacgt ttccattctt ccagagaacg aattaaaagt
     2041 gataaaaata aatcttcctt attgcgaaaa tattcataaa tagtaccttt gccaatatga
     2101 gcaactttaa ttacatcctc aacataagcc tcataatagc ccttctcggc aaataccttt
     2161 tttgcgcaat caagaatatg ctgcctgcgc aactctgctt tcattgcaac ccctattccg
     2221 tttattttta taacattttg aatatttaaa aattctttat atttggtcaa taacttttta
     2281 ttgatagtat ttattgttca taattttgat ccaattttag cagtgcattc tttaactcat
     2341 caagtgtaaa tggtttttgc aaaaatactg agtgcttaat acctttcgtc tcatctcggt
     2401 aagaatcatc tgaatatcct gaaataaaaa ttactttcat atccggtata attaatttta
     2461 atctgttaaa cagttcaata ccattcatct gcggcataac tatatcagta attaatatat
     2521 caatgttgtt tttatagttc tctgctactt gaagcatctc aagaggatca ctaaatgcat
     2581 caacgtcctg atcaagtaac aaaagcatct tttttagcat atctaatatg atcaagtcat
     2641 cttcaaccac taaaatttta tttttagata taccaatacc tctatcttct gtcctaccat
     2701 tatctcttac atgaccagat aattgtacct ctggcaattg ctcaacatat ttagggaaaa
     2761 agatatttac tacagttcct ttgtcttgtt cactatagac attaataaat ccattaaact
     2821 gttttactat gccatacact gttgaaagtc caagccctgt tcctttgcca acttcctttg
     2881 ttgtgaaaaa tggttcaaaa agcctttcca acgtttcctt atccatccct gaaccatcgt
     2941 caataaattg tatcatgaca tagtctccgg gtttacaccc gggatgtatt gtgcagaatt
     3001 cttcatcaag atatctattt ttagtaacga tctttatatt cccaacatct ttaatagcat
     3061 cacgtgaatt tgaagccaaa ttggtcagaa tttgaacaaa cgttgcggta tcaatccata
     3121 tatcatatac atcatctgcc agttccagtt gcacttttat gttttcgcca accattgccg
     3181 ctaacatctt ttcagttgac cggataatat aattaggatt tacccgtatt ggatttactt
     3241 tctgctggcg tgaaaatgtt aaaagctgcc gtactatttc agcacttcgt tttgtggcat
     3301 ttgatatttc ttctaaatct gaatatatag gattatcttt ttctattttg attttaatta
     3361 actctgcata ccctgaaata atgctcaata tattattaaa atcatgtgca atcccgccaa
     3421 ccaatctccc aattgcttcc aatttttgcg cttgccgtaa ctgctcctct aatttttctt
     3481 tttgggcttc cgcttcaagc tgatacgtca tatcttctac aatggccaat ccaccaacta
     3541 ttttattttc tacaatgagt ggtgaaaata atgcccttgc aggtgttatt ttatctgcag
     3601 ttactgattt gtaccaacca ttatattcag attgtatacc ctgcaggcac ttattaacag
     3661 catcaacaac ccgctcatca ggcaacttta gagtatttaa accaatcaaa gcttccctgg
     3721 aagaaccaat tatcttaaca aaattatcgt tacaggctgt aataactcca ttttcatcaa
     3781 atgcatagat ccccattggg gcattttgaa aaattagtct aaataattcc tcgttctttt
     3841 ttaacgcctc ctctgcctgt ctttttgcag taacatcatg aataattgag aataaatact
     3901 gttttccttt aatggtaatg gcactgctgt atacatcaac atcgcgtatg cttccatctt
     3961 ttcttctatg cttaaattca aaatacacac gtttacaggc tcgtgcctct tccatacgtg
     4021 ccctaatctc ttcgggggtt aatgtgttaa tttgactaat ttgcatcgat tgtaattctt
     4081 ttctactcca cccataaaac tccactgcag cctcatttgc atcaacaata tctcctgtat
     4141 cgggatcaat gataagcata actgctttac tgtttttaaa aatggttcta tatcgttctt
     4201 cactttcttt cagttcatgt tcaactttct tacgttgagt gatatcgctt attgatccaa
     4261 ataaagcagc tattttaccc ttttcatctc tgattacccg gattttaata tctccccata
     4321 caaccgtttg atataaagta atgattctgc attcaactga aataaaatta ttgtcaccag
     4381 gctcttcaaa catcttgtca tataggtaaa catcatcttt atgaatatat gaattcaaag
     4441 gtgtattaat tatttcatcc tgtttatagc caagtacaac ctcaacattg ggacttatcc
     4501 atgtaattgt ctttgtagat ggatccatgc tgaagacaac agacggattg acactgagaa
     4561 catcatgcag aagatcagcg ctggcctgta aattttgcag tgccagtgtt ctattttcaa
     4621 tggttttaaa tataagagct atcgcacata aaccagaaga aagtactatt aaatactgga
     4681 tgcctatagt agctattaca gtaatcccac gtggccaggg taataatagc tggcacagta
     4741 aaaccagaac atgtgttata accgcaaata gaacgattcc atacaggggg atgtttgttg
     4801 gttcattttt aaaaattcga cgtacaacca atccactcaa ggctgaaaca atgaccgatg
     4861 caacgccaac ataaactcca ggaccaccca gaataatccg gtagcttcca gcagtaagcg
     4921 atgcaataag tgatgcgata cct
//
LOCUS       DAOSZP010000100         4923 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_14250_length_4923_cov_2.952753, whole genome shotgun sequence.
ACCESSION   DAOSZP010000100 DAOSZP010000000
VERSION     DAOSZP010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4923
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14250_length_4923_cov_2.952753"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..400
                     /locus_tag="PK348_03255"
     CDS             <1..400
                     /locus_tag="PK348_03255"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HXK65263.1"
                     /translation="IEATALQRDGKLLVVGESFDGNRFQIVIGRYETGVMTGTNNSID
                     PVTSIQVYPNPTSDFVHVSFTLEEALPVEIQLFDRTGQMVHSFSSAARLAAGEYQETY
                     AVPVNLPAGIYHLAVNTGHQAAGINLLIRP"
     gene            430..636
                     /locus_tag="PK348_03260"
     CDS             430..636
                     /locus_tag="PK348_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65264.1"
                     /translation="MYNLLLNNDYTVYGLARQVFKNLKGINLVLDLYLSLSETYTHLE
                     AMCEEGDVIKYNFDNTYYYTATLL"
     gene            703..2148
                     /locus_tag="PK348_03265"
     CDS             703..2148
                     /locus_tag="PK348_03265"
                     /inference="COORDINATES: protein motif:HMM:NF013132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HXK65265.1"
                     /translation="MKLSHKKALYLFLAIGIALAIGLLPIEYWQNTGDKSIQLTTQGK
                     YSLAVLAFAVIGWVTEIMPFAITGLFSLSLLVITGCANLADLVKDGFGNQVIVFFIGV
                     MIIGAIVNETSFVSIITQKLYKRYCSQPKTLIFIILAIGMILAGFVTEMAAAAMVLPF
                     AVSILKRAHIEPLKDNYGKAMMIACGWGPVIGGISTPAGSGANPLTVGFLKDLAHIDL
                     SFAQWMGIGFPAALLMLPFAWYILVKTFNIESVSLHVDTEHNEKIQIPLHDIIIGILF
                     ILTILLWIVEPVLHNLFPSLQYITMSFVAITIACCMFLPPFAQLSWHKVEHAINWGAI
                     ILIVSGLALGTTIYKTGAAKWLAWVLFKNLNLLHPVIMVFAVVIGVSIVKVMFSSNTV
                     TGIIMVPLLIALSGTIGIDPALVAIPAGLTSSLSFILVTSTPTIVIPYTAGYFTIADM
                     VKPGIYMTIVSSICVTISFLIFGSLFNIITW"
     gene            2217..3059
                     /locus_tag="PK348_03270"
     CDS             2217..3059
                     /locus_tag="PK348_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020072010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HXK65266.1"
                     /translation="MNTMSTEQLEQRAKEFRYVITDMICRAGSGHLGGSLSLVEIVTT
                     LYYRIMNIKPENPAWEDRDRLVLSKGHAGPVLYVVLAYLGFFPKSWLKTLNQNGTNLP
                     SHVDHNKTPGIDMTAGSLGQGLSCACGMAYAAKLNKKSYNVFCIIGDGESNEGQIWEA
                     AMFAGHNKLDNLIVICDYNKMQIDGKTDEVVTLEPLADKWRAFNFEVFEMDGHNWDDI
                     YSTINKAISVKGKPAMIIAHTIKGKGNAEVENKVASHNIKINNQSDYDKYMKGLGYTM
                     TLPY"
     gene            3175..4119
                     /locus_tag="PK348_03275"
     CDS             3175..4119
                     /locus_tag="PK348_03275"
                     /inference="COORDINATES: protein motif:HMM:NF014798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HXK65267.1"
                     /translation="MSYHDMEMRAVYGLTLCELMEHDKNVLCLEADLSKASGTNPAVC
                     TAFPENFINVGVAEANMIGLAAGLAREGKIPFCASFTCFASRRVYDQITISVAYANTN
                     VKIVGTAPGITQGPNGGTHMDFLDLAIMRAMPNMHVYSPCDVYELRAVMKYMAAHHQP
                     TYMQLLRPKMPVVFNESFTFDPNKATKISEGSDVTIVTTGYTTHLAINAVEQLKKEGI
                     KAEHLHYSSIKPFDEDLLIQSAKKTGCVVTVENQNIIGGLGGAVCEVLSEKLPVIVKR
                     LGIPDAFGEVATEEYLLNKHGFNVEHIITACKDMIKSK"
     gene            4133..4606
                     /locus_tag="PK348_03280"
     CDS             4133..4606
                     /locus_tag="PK348_03280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HXK65268.1"
                     /translation="MNFVIGSTRAGLQLKRAIIDYLVKQGHQIDDVGMKDEANFVPYH
                     IAAANVASLVSQGKYEKGIVICGTGAGSAIVANKFKGVYAVHCTNSFEAKKASAINNA
                     NVLVLAEWLTPANHAFEIINDWLSTPFGSGFEEEWQKFLANCIDEIKDMENKNFK"
BASE COUNT         1611 a          764 c         1007 g         1541 t
ORIGIN      
        1 tattgaagcc acagccctgc aacgtgatgg taaattgctt gtggttggcg aaagttttga
       61 tggcaaccgt tttcaaatag ttataggtcg atatgaaacg ggtgtgatga ccggaacgaa
      121 caattccata gatccggtta catccattca ggtttatccc aaccccacca gtgactttgt
      181 ccatgtatcc ttcacccttg aggaagcctt acccgtggag attcagttat ttgaccggac
      241 gggtcagatg gtgcattcat tttcatcagc ggcgcgcttg gcagccggtg aataccagga
      301 gacctacgca gtgcctgtga atttacccgc cggtatatat catcttgcgg tgaacacggg
      361 ccatcaggct gctggaatca atctgttgat cagaccttag gtgtataaac aggatttcga
      421 aagaaacgca tgtataattt attattgaat aacgattata ccgtttatgg actggcacgc
      481 caggtattta aaaatcttaa aggtataaat ctggtgcttg atctgtattt atcattatcg
      541 gaaacatata ctcacctgga agccatgtgt gaagagggcg atgtaataaa atataatttt
      601 gataatacat actattatac tgcaacatta ttataaatta caattgacat aatatatcga
      661 tatcgattta ttgtatctaa ttattacggt aaattctata ctatgaaact atctcacaaa
      721 aaagcactat acctcttttt agctattgga atagctttgg caatagggtt gttgccaatt
      781 gagtattggc aaaacacagg tgataaatcc atacaattaa caactcaggg taaatattct
      841 ctggcagtat tagcgtttgc agtgataggg tgggtaactg aaataatgcc atttgcaatt
      901 acaggcttat tttcgctttc gttactggtt ataacgggat gtgcaaatct tgctgatctg
      961 gtaaaagatg gttttggcaa tcaggttatt gtttttttta ttggagttat gatcattgga
     1021 gctatcgtta atgaaacttc ctttgtttca ataattacac aaaaacttta taaacgatat
     1081 tgttcacaac ctaaaacatt gatttttatt atacttgcta ttggcatgat tttagcggga
     1141 tttgtgacag aaatggcggc agcagctatg gtgttgcctt ttgcagtttc aatcttaaaa
     1201 cgagcacata tagagccact gaaagataat tatggtaaag caatgatgat tgcatgtggt
     1261 tggggaccag taataggtgg catatcaacc cctgcaggtt cgggtgctaa tccgcttacc
     1321 gtgggatttt taaaggatct ggcgcatatt gatctttcgt ttgcacaatg gatgggtatt
     1381 ggttttcctg cagcactgtt aatgctacct tttgcatggt atatactggt aaaaacgttt
     1441 aacatagaat cggtaagttt acatgtagat actgaacata atgaaaaaat acaaatccca
     1501 ttacatgata taataattgg gatattattt attcttacaa tattgttatg gatagtggaa
     1561 cctgtactgc ataatctatt cccatcgtta cagtatataa ctatgtcctt tgttgctata
     1621 acaattgcat gttgtatgtt tttgccgcct tttgcgcagt taagctggca taaggttgaa
     1681 catgctatta actggggcgc aattatttta attgtttcag gtttagcgct gggaacaaca
     1741 atatacaaaa cgggagcagc taaatggctt gcatgggtac tattcaaaaa tcttaatctt
     1801 ttacatcctg ttattatggt ttttgcagtg gtcattgggg tttctattgt aaaagttatg
     1861 ttttcaagca atacagtaac cggtatcata atggtaccgt tactcatagc actatctggt
     1921 actattggga ttgatcctgc gctggttgca attccagcag gattaacatc atcgctttct
     1981 tttatactgg taacatcaac gccaacaatt gttattcctt atacagcagg atatttcaca
     2041 attgcagata tggtgaagcc gggcatatat atgaccattg tttcatctat atgtgtcaca
     2101 ataagttttt taatttttgg ttctttgttt aacatcataa catggtaaat atttttttaa
     2161 ttagtaatat tcaaaaaaat tattttatgc gatagtaact gttaatggag gagttcatga
     2221 atacaatgtc aacagaacaa ttagaacaac gtgcaaaaga gttcagatat gtaataaccg
     2281 acatgatatg cagagctggt tcaggccatt tgggtggttc attgagttta gtggagatag
     2341 ttaccacact atattatcgt atcatgaata ttaagcctga aaatccagca tgggaagacc
     2401 gtgacagact tgttttatca aaaggacatg ccgggccagt cttgtatgta gtacttgcat
     2461 atttaggctt tttcccaaaa agctggctaa aaacattaaa ccagaatggt acaaatttac
     2521 caagccatgt agatcataat aaaacgccag gtatagacat gacagcaggt tcattggggc
     2581 agggtttatc atgtgcctgt ggtatggcgt atgctgctaa actcaataaa aagtcatata
     2641 acgtcttttg tattattggt gatggtgaaa gcaatgaagg gcaaatttgg gaagctgcaa
     2701 tgtttgcagg acataataaa ctggataacc tgattgtaat ttgtgattac aataaaatgc
     2761 agattgatgg aaaaactgat gaagttgtaa cacttgagcc cttagctgat aaatggcgtg
     2821 catttaattt tgaagtattt gaaatggatg gtcataactg ggatgacata tattccacca
     2881 ttaataaagc aatttcagta aaaggaaagc ctgcaatgat cattgcccat acaataaaag
     2941 gaaaaggcaa tgcagaagtt gaaaataaag tggccagcca taatattaag atcaataatc
     3001 aatcagatta tgataagtat atgaaaggtt tgggttatac aatgacgttg ccatattgat
     3061 tatacagggt atttttgcaa gggcataatg cttattagta atcaatgatc aatggccatt
     3121 acagtataat gattaattca aaatgattaa tatatttgtt taaggagaaa aacaatgagt
     3181 tatcatgaca tggaaatgcg tgcagtgtat gggttaacat tatgtgaatt gatggaacac
     3241 gataaaaatg ttttatgcct ggaagctgat ttaagtaaag cttcaggaac caatccagca
     3301 gtatgtacag cattccctga aaattttata aatgttggcg ttgccgaagc aaatatgatc
     3361 ggattggcag caggtttagc acgtgaaggc aaaataccat tttgtgcaag ttttacctgc
     3421 tttgcatcac gaagggtgta tgaccagatt accatcagtg tggcgtatgc caataccaat
     3481 gtaaagatag tgggaactgc gcccggtatt acacagggac caaacggtgg aacccatatg
     3541 gactttctgg atttagcaat tatgcgtgct atgcccaata tgcatgtata cagcccctgt
     3601 gatgtatatg aactgcgtgc tgtaatgaag tatatggcag cacatcatca gccaacctat
     3661 atgcaattac tgcgacccaa gatgccagta gtatttaatg aaagttttac gtttgatcca
     3721 aataaagcaa caaaaatttc agaaggcagc gatgtaacaa ttgtaacaac gggatataca
     3781 acgcatttgg ctataaatgc cgtagagcaa ttaaaaaaag aaggaattaa agcagagcat
     3841 cttcactaca gctccataaa accttttgat gaggatttgc tgatacaatc agcaaagaag
     3901 actggttgtg ttgtaactgt ggaaaatcaa aatatcattg gtggattagg tggggctgtc
     3961 tgtgaagtat taagtgaaaa acttccggta atagtgaaac gtctgggtat tcctgatgcc
     4021 tttggtgaag ttgcaacaga agaatattta ctcaacaaac atggatttaa tgtagaacat
     4081 attattactg catgcaagga tatgattaaa agcaaatagg gggaattaaa caatgaactt
     4141 cgtcattgga tcaacacgtg ctggattgca gcttaaacgt gctataattg attatcttgt
     4201 aaagcagggt catcaaatag atgatgttgg tatgaaagat gaagcaaatt ttgtgcccta
     4261 tcatatagca gctgcaaatg tggcatcctt ggtttcacag ggaaaatatg aaaaaggcat
     4321 tgtaatatgt ggtacgggag cgggtagtgc aattgttgcc aataaattca aaggtgtata
     4381 tgcggtacat tgtaccaatt catttgaagc aaaaaaagca tcagctatta acaatgcaaa
     4441 tgtactggta ctggctgaat ggcttacacc cgcaaatcat gcatttgaga taataaatga
     4501 ctggctttca acaccatttg ggagtggttt tgaagaagaa tggcaaaaat tcttagcaaa
     4561 ctgcattgat gaaattaaag atatggaaaa taaaaatttt aaataagtaa tgaccctaaa
     4621 tccaaacata agtaaatact gtaaaaagca aaagcgcgat acttattgtt tatatacttt
     4681 gttataagat gcaataagta atgtaataaa tatactatct ttatttctct aatgtgacat
     4741 aatatatctt atcggaagta atatatgaag tataaaagga taaatgttta tagacaaaat
     4801 tatttctttt acggtatatg gctgatagtt acaatattac ttattattcg attgtgtgat
     4861 acatatcatt aatattcatt ataggattta gcagtgaaga aaatatattt aatattagtt
     4921 tta
//
LOCUS       DAOSZP010000101         4901 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_14332_length_4901_cov_2.718531, whole genome shotgun sequence.
ACCESSION   DAOSZP010000101 DAOSZP010000000
VERSION     DAOSZP010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4901)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4901)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4901
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14332_length_4901_cov_2.718531"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1582)
                     /locus_tag="PK348_03285"
     CDS             complement(<1..1582)
                     /locus_tag="PK348_03285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65269.1"
                     /translation="MLPYWHTSLDFIQEESQTDSLNEQVTATRRGITSIKQFHFASTL
                     SSPDTAIPKLDVLYNYENFTNKQAYYQDVNSFDNDTSKITHQATVGVQQLLHDVLGGD
                     LSYRLLLDTSFKEDIFKENSQSVATGLNTQKKQRESCSMNINYQLTHFFISPTIQILS
                     EEFITYSGTVTDINPALSQEISSEYHIPFVYGDSIRFIERLKKSSLSFGLKSYKLINP
                     SITYEFSYFENQFKDLQPYDTWMTFGFNRTRSTQGFLSSTIAIPINLQTIFPSIKSCS
                     FNYTRASTLNEINVPYEGESINFYEEKYGVSRYLNQCANPIYNIFHYPPWHFFKGRSN
                     YAQGRDFVSHTLSSQPEVNGAPFSDYNNYFRLLDNASFSINWELSPFVLFINSSIHSV
                     SDRNGVNTASQQVVTYTLVSSLSCDLMKLFSFGFFRPNSPDLPYHSATALLEYQWNRY
                     LRITSNILQDEYTPSIGATFKWDRSSIAAKFGISYRTQKWHEFIPLDDNERSAKDDIY
                     FYNMMVQSPFTNIDKGYTF"
     gene            complement(1597..>4901)
                     /locus_tag="PK348_03290"
     CDS             complement(1597..>4901)
                     /locus_tag="PK348_03290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65270.1"
                     /translation="TPEQTMQLHIEGKAGKRLTLYIDHDSQQRTNTYSMQYKAVEEDE
                     VLREINAGDITIKVNNSKYAVYDDTTSKGMGIDFTIKKGNLQVKAFGSVAKGVSEVER
                     FKGNYSSTSITLQEYQYTKATYYQLEPFKRYDNLDSPPSNMYTTITWTSAPVNPSTFT
                     PYSVGIDSSGFELYADDAITQNKSGLLELHRWDNPSIILGYFRKLQQGSDYTINFSTG
                     TIALLKAFKPQAKLFAVYTRNGGTTQTSDPSAITNHPQFPGKICVFIKYGPLLHEDVN
                     YNGTLESGEDTNNDGVLNIDIYEIRGIYTTGLQNIRAEDVKISFFSNLFPMSRSDIQK
                     LGNFIIDYNTGAIIFKLREPFKQLLPAQSQKIIYNEAQTQAYTVSQYKIGIAGYSQSR
                     GFQLKHVNIIPDTIRVKINGIQIAESLYSVDPTSGFFQFTNASNPVITPETDIEISYQ
                     YLPFEGQAQQFIGGMRADYALNKNINVGGTILYTTQGIASTVPLLGNEPTQTTVVEAD
                     SSVEVSPHDISAFASKIAQSDISVPVLFKGYAEYAKSYHSINTFGKGLIDDFESIYSG
                     LTVSMSEKDWVLGSLPDSVPQNLRARIYYKFYRDINNPETLYGLNVTPIDVPYSKKPG
                     PYNVATGHIAPSIQSEQSQRSLGLNFDFSSGDYISIVTRTLSQKPSDLSNLEYIEIWY
                     KSTGGTGTVDMYLDIGSINEDSDSDGILDTEDSNRNGFLDYDTNRNISEDTGYTFDNP
                     FTTVVGCGPGLSSTTIGDGVLNTEDLNDNGMLDTGEQYIRIPGLSTPITIDCANTTWQ
                     KVRIYINKTDPALYTASPDAFEDILKKIYAIRLILQKNSATTGTILIDTIRFVTMHWQ
                     VDSIDGISGTDTDKVKLTLIDSFNDSEYAQESFARTKSDVYTSLYGDKSKKELSQTME
                     SALNVTYSSITSATIKRKFYKPMDLRFYRNVHCWINVRNYTPGDTLIFRLHSSDNDYI
                     EYSYNPQFIQTWEDIVLSLQYQDTNAHFIQKEGNPDLKRIIAITVSVQNTTTSGQFWL
                     DDIYASDPLTLEDTACWYEGTMKITKPVARTQAGTPVLSDITLSYLKKQHGNNFYTIG
                     QPYNIFLKTITR"
BASE COUNT         1417 a          852 c         1023 g         1609 t
ORIGIN      
        1 aaaatgtgta tcctttatca atatttgtaa atggtgattg taccatcata ttataaaaat
       61 agatatcatc ctttgccgat cgttcattat catcaagggg gataaactca tgccatttct
      121 gggtacggta gcttatacca aattttgcag caatgcttga tctgtcccat ttaaatgttg
      181 ctccaatact gggtgtatac tcatcctgca gtatattgga agtaatacga aggtatctat
      241 tccattgata ttctagtaat gcagttgcgg aatggtacgg aagatctgga ctgtttggcc
      301 tgaaaaaacc aaaggaaaat agtttcatta aatcacatga taatgatgaa acaagagtat
      361 aggtaacaac ctgctgggaa gcggtattga cgccgttgcg gtcagaaaca gaatgtatgc
      421 tgctgtttat aaataaaaca aatggcgata gctcccagtt gatactgaat gaagcattgt
      481 ctaatagcct gaaatagtta ttatagtctg agaatggtgc cccattaact tctggctgag
      541 acgataacgt atggcttaca aaatcacgcc cctgagcgta atttgatctc cctttaaaaa
      601 aatgccatgg tggatagtga aaaatgttat agatggggtt ggcacattga ttcaaataac
      661 ggcttactcc atatttttct tcataaaagt ttatggactc gccttcatag gggacattaa
      721 tctcattcag tgtgcttgcc cgagtatagt tgaatgaaca actttttatg ctggggaata
      781 tagtttggag atttatgggt atagcaattg tactggatag aaatccctgt gtagaacggg
      841 tacgattaaa tccaaaagtc atccaagtat catagggttg caggtcttta aattgatttt
      901 caaagtatga aaattcatag gttatagaag ggtttattag tttatagctt tttagaccaa
      961 aggaaagaga actttttttc agacgctcaa taaatcgaat actatcacca tatacaaaag
     1021 gaatgtgata ttctgaacta atctcctgtg ataatgctgg gtttatgtca gttactgttc
     1081 ctgagtaggt gataaattct tctgatagaa tctgtatggt gggtgaaata aaaaaatgag
     1141 ttaattgata attaatattc attgaacagg attcacgttg ttttttttgt gtgtttaatc
     1201 ctgttgctac tgattggctg ttttctttaa atatatcttc tttaaaagaa gtatcaagga
     1261 gcaacctgta cgataaatcc ccgcccaata cgtcatgtaa caattgctgc acgccaactg
     1321 ttgcctgatg cgttattttg ctggtgtcat tgtcaaacga gtttacatcc tgataatatg
     1381 cctgcttgtt ggtgaagttt tcataattat ataatacgtc aagctttgga attgctgtat
     1441 cgggagaaga gagggttgag gcaaaatgga attgttttat actggttatt cctcttctgg
     1501 ttgcggttac ctgctcatta aggctatcag tttgtgattc ttcctggatg aaatcaagtg
     1561 atgtgtgcca gtacggaagc acctggcagg ttactgttac ctggttatag tcttcagaaa
     1621 tatattatat ggttgcccta tcgtataaaa attattccca tgctgttttt ttaaatacga
     1681 tagagtaata tcggatagca ctggagtccc tgcctgggtg cgagcaactg gtttggtaat
     1741 tttcatagta ccttcatacc agcaagcggt atcttcaagt gttagggggt cacttgcata
     1801 gatatcatca agccagaatt ggccactggt agtggtattc tgtactgaaa ctgtgatagc
     1861 tatgattcgt ttaagatcag gattaccttc tttttgtata aagtgtgcat ttgtatcctg
     1921 gtattgcaat gatagtacaa tatcttccca tgtctggata aactgtggat tgtatgaata
     1981 ttcaatatag tcgttatctg aagaatgcag acggaaaata agcgtgtcac cgggagtgta
     2041 atttctgaca ttaatccagc aatggacatt acggtaaaac ctgagatcca ttggtttata
     2101 aaattttctt tttatagtgg ctgaagtaat agaactatat gttacattca gggcagattc
     2161 catagtctgg ctgagttctt ttttactttt atcgccatac agactggtat aaacatcact
     2221 tttagtacga gcaaaagact cctgtgcata ttcggaatca ttaaatgaat caataagggt
     2281 aagctttact ttatcagtat cagtaccact tatgccatca atactatcca cctgccagtg
     2341 cattgtaaca aagcggatgg tatcgataag gattgtgcca gttgttgctg aatttttttg
     2401 taatataagt ctaatagcat atattttttt taatatatcc tcaaaagcat ctggtgatgc
     2461 ggtatataaa gctggatcag ttttatttat ataaatacgg actttttgcc atgtggtatt
     2521 ggcacaatct atggtgattg gggttgataa tccaggtatg cgtatatact gttcaccagt
     2581 atcaagcatg ccattgtcat ttaaatcttc ggtgttgagc actccatcgc caatagtggt
     2641 agatgataga cccgggccac agcctacaac tgttgtaaat ggattgtcaa acgtatatcc
     2701 tgtatcttca gaaatatttc tattggtatc ataatctaaa aatccgttgc ggttactgtc
     2761 ttcagtatca agaataccgt cactgtcact gtcttcattt atagaaccaa tatcaaggta
     2821 catatcaaca gttcctgtgc cgccagtact tttataccat atctcaatat attctaaatt
     2881 agataaatca gagggttttt gtgatagtgt acgggtaaca atagaaatat aatcccccga
     2941 cgaaaaatca aagtttaaac ctaaagaacg ctgcgactgc tcgctctgga ttgacggtgc
     3001 aatgtggcca gtagcaacat tgtaagggcc gggctttttg gagtatggca catcaatggg
     3061 agtaacgttt aatccataga gtgtttcagg gttattaata tcacgataaa atttataata
     3121 tattcgggct cgtagattct gtggcacact gtcaggcagc gaacccagta cccagtcttt
     3181 ttcggacatt gatactgtaa gccccgagta aattgattca aaatcatcaa ttaatccttt
     3241 gccaaaggta ttaatgctgt gataactttt tgcatactct gcatagcctt tgaacagtac
     3301 cggtacggaa atatcggact gtgctatttt tgaggcaaat gctgatatat catgagggct
     3361 cacttcaacg gaactatcgg cctctacaac agttgtttgt gttggttcat tgcctaacag
     3421 gggaacagtg gatgctatac cctgagtggt atacagaatt gttccaccaa cattaatatt
     3481 tttattcaga gcataatcag cccgcatgcc tccaataaat tgctgagcct gtccttcaaa
     3541 gggtaggtac tggtagctta tttcaatgtc ggtttcaggg gttattaccg ggttgcttgc
     3601 attggtaaac tggaaaaaac ccgatgtggg gtcaacgctg tacaagcttt cagcaatctg
     3661 gatgccatta atcttaactc gtatggtgtc agggatgata ttgacatgtt tcaactgaaa
     3721 gcctcgtgac tggctgtaac ctgcaatgcc aattttatac tgggatacgg tatacgcctg
     3781 tgtttgtgct tcattgtaga taattttttg ggattgtgcg ggcaaaagct gtttaaatgg
     3841 ttcacgaagc ttaaatataa ttgcgccagt gttataatca ataatgaaat ttcctaattt
     3901 ctgtatgtcg gatctggaca tggggaaaag attgctaaaa aacgatatct taacatcttc
     3961 agcgcgaatg ttttgtaatc ccgtagtata tatcccacga atttcgtata tatcaatatt
     4021 taatacgcca tcattgtttg tatcttcccc tgactcaaga gtgccgttat aattgacatc
     4081 ttcatggaga agtggaccat attttataaa tacacatatt ttaccaggga attgtggatg
     4141 attggtaatg gcagaagggt cggaagtttg tgtagttcca ccattgcggg tatacacggc
     4201 aaagagcttt gcctggggtt taaaagcttt aagcagggcg atagttcctg tagaaaagtt
     4261 tatggtataa tcagaaccct gctgtaattt tctgaaatag cccaaaataa tagaaggatt
     4321 atcccatctg tgaagttcca gcaatccgct tttattctgt gtgatagcat catcggcgta
     4381 cagttcaaaa ccagaactat cgataccaac agaatagggt gtgaaggttg atggattaac
     4441 gggtgctgat gtccatgtaa tggttgtata catgtttgat gggggagaat ctaaattgtc
     4501 atagcgtttg aacggttcca gctgataata ggtggctttt gtgtactgat attcctgcaa
     4561 tgtaatgctt gtactggaat agttgccttt aaaacgttct acttcactga cgccttttgc
     4621 gaccgaacca aatgctttta cctgtaaatt gcccttttta atggtaaaat ctatacccat
     4681 gcctttggat gtggtgtcgt catatactgc atattttgaa ttattaactt taatggttat
     4741 atcaccagca ttaatttcac gtaatacttc atcctcctca acagccttgt attgcatact
     4801 ataggtattg gttcgctgct ggctatcatg gtcaatgtat agggtaaggc gcttgccagc
     4861 tttgccttca atatgtagct gcatcgtttg ttcaggtgta a
//
LOCUS       DAOSZP010000102         4868 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_14456_length_4868_cov_3.091211, whole genome shotgun sequence.
ACCESSION   DAOSZP010000102 DAOSZP010000000
VERSION     DAOSZP010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4868
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14456_length_4868_cov_3.091211"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(329..742)
                     /locus_tag="PK348_03295"
     CDS             complement(329..742)
                     /locus_tag="PK348_03295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65271.1"
                     /translation="MPKLLCDFVNSMIEEWGEDNPFYGLKNTGQLVEQWTNLDGLEIF
                     YSYVRNSKWVTVTVLPTETGIHPVSNSVYKWKGYINEYIAETAVWWAFELLTEMEAKK
                     FMIQHKPEVKFTFIRLGHPYELVVKFDGYYWVVED"
     gene            complement(706..1407)
                     /locus_tag="PK348_03300"
     CDS             complement(706..1407)
                     /locus_tag="PK348_03300"
                     /inference="COORDINATES: protein motif:HMM:NF023227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65272.1"
                     /translation="MEKDFLAPLSIAKLYGVGIKTQKYLESSGYKTIGDIQRVDENFL
                     QSLLGKWGVVLHQFALGIDRRPVEPYSQAKSVSEETTFDVDTDDEDSIIFTLVELCDR
                     VIRRVRAAEFSFKTVTLKIRLEGFETYTRSKTLQQHARDMATLQKEIMRLYISFGRGK
                     RKVRLLGVKVSNLLCNADVQMNLFENQQKVEKIEEVIDSIHNRGLNVLRASVIKPPKR
                     RGRVCQSFYVILSTV"
     gene            complement(1380..1922)
                     /locus_tag="PK348_03305"
     CDS             complement(1380..1922)
                     /locus_tag="PK348_03305"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF013015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HXK65273.1"
                     /translation="MKDRIILHVDMDAFFTSVEQVDNPSLKGKPVVVGADPKGGRGRG
                     VVSAASYEARRFGIHSGMPISQAYKLCPGAVFLPPRFDRYVELSHIIMKIFERYSPCI
                     EQISIDEAFLDCTGTLKLFGNVENIARNIKTDVFTQTGLTCSIGIASNKLVAKIASDL
                     QSLMGLQYVPVEWKKIFWHH"
     gene            1978..3273
                     /locus_tag="PK348_03310"
     CDS             1978..3273
                     /locus_tag="PK348_03310"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXK65274.1"
                     /translation="MTIVLFTVNFTTMSENIQYIYRSLSTLLPKETERKLVLITGARQ
                     TGKTTLAKKVYQHLRYINFDSPEIRQTVKNISTEDWAKDIGTAILDEAQKEPLVFEKI
                     KYSYDEQSLTFSVILGSSQILLLKKIRESLAGRIWIYELWPLMQSELVNITSWNVPLF
                     IKILESDTLDALLQQQPTVIVGQQMSNCKKAENYILQWGGMPALIHIYQNNEKHQWLK
                     NYIYTYLERDLSDLARLQDLEPFEKFQKLSALRCSSLLNYSELSRDAGISVDTARRYL
                     EYLKLSYQVILLQPYMENITSSTVKTPKIYWIDNGIMRQLTGYTSDTVTGQLYENHVI
                     SEMHKCIKTLQKPASLYFYRTRSGLEVDVVIETAKGLIFCEIKNREEIVKSDFTAIKS
                     IAQKLGKKFLGGLIIYRGERLYQLSHPSMWAIPSWRLFT"
     gene            complement(3335..4696)
                     /locus_tag="PK348_03315"
     CDS             complement(3335..4696)
                     /locus_tag="PK348_03315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HXK65275.1"
                     /translation="MTSFADDVNTLRVVCQANTNPPVVSNAQVTRVVNALEVYCEFDI
                     VGTQYYIKHTKPDGTSTTTPFATAVLNSNNKPHVRKTFDMTGQAYGVHTFTIYAKDMY
                     NTTTYEVYTLQKDWQKLNIANAPVIWPKVEPGIGFITISWPAWYKEAEFGKYVIQMER
                     PVKTIEVTEYTTTSYKLTGLTPGDTYSGTFKVYDKAGNIIYSMQNPPRFFGHVGSIAP
                     KISNVSITPNPAGTNQEIQFLATVSDPDTNLTHVNLVLQEYVETTDSKGNLITQMGKT
                     ITLIDTDVYNTKQYQVIHHFVTSEPGKHKLILKATGASPNEFTQVEYSVIVQQGLDPS
                     TPKISIEGPNASLKQILTDGYQLKINIVSVDAESENIKAVIDWGDGTVKSEMIERESL
                     SISTTGKEGSPDFRKILSGSVSAKYYYQKPGVYVITVYIQSELKGKKLQSQPVRLKVR
                     VTE"
BASE COUNT         1479 a         1011 c          814 g         1564 t
ORIGIN      
        1 ccagttattt cggctcccct tctgtcattt cgagtgctcc acttctgtca tttcgagtac
       61 tcctcttcta tgtcatttcg agcgacagcg agaaatcttt tgcgtgcatt tcaagatttc
      121 tcctcacttc gttcgtcgaa atgacatggt ggatggtgag tttatcgaaa taacaaatga
      181 attctttggt catgcaaaat gatatggtgg actcttggct tgttgaaatg caaatgaacg
      241 ctacagtata taccgatact atacaaaagt tcttttaaag gctatagatg agattactgt
      301 gacaggcata aagaatcata gaattgtatc aatcttcaac aacccaataa taaccgtcaa
      361 attttacaac caattcatag ggatgtccca accgtataaa agtaaacttt acctcaggtt
      421 tatgttgaat catgaatttt tttgcttcca tttcagtcag tagttcaaat gcccaccaca
      481 cagcagtttc agcaatgtat tcatttatat atcctttcca tttataaaca ctattgctta
      541 caggatgtat gcctgtttca gtaggtaaca ccgtcacggt aacccatttg ctattgcgca
      601 cataactata gaatatctcc aatccatcca ggtttgtcca ttgttccaca agctgtccgg
      661 tattcttcaa tccataaaat ggattatcct caccccactc ttcaatcata ctgttgacaa
      721 aatcacataa aagctttggc atactctacc ccttcttttt ggcggtttta tcaccgaagc
      781 tcgtaacaca ttaagtcctc tgttatgaat actatcaata acttcctcaa ttttttcaac
      841 cttttgttgg ttttcaaaaa ggttcatctg cacatcagca ttacacagca aatttgaaac
      901 cttaacccct aaaagccgca ctttcctttt ccctcgacca aatgaaatgt ataaccgcat
      961 aatttccttt tgcagtgttg ccatatcacg ggcatgttgc tgtaatgttt tactgcgtgt
     1021 atatgtttca aaaccttcca atcgtatttt taacgtcact gtcttaaaag aaaattcagc
     1081 ggctctgacc cttcgtataa cacgatcaca cagttcaacc agcgtaaaaa taatactatc
     1141 ttcgtcatca gtatcaacat caaaggttgt ttcttcactc actgactttg cctgagagta
     1201 tggctcaaca ggacgtctat caatacccag tgcaaactga tgtaacacta caccccattt
     1261 tccaagcagg ctttgcaaaa agttttcatc aacacgctga atatccccaa tggttttgta
     1321 tcctgatgac tctaaatatt tctgtgtttt tattccaaca ccatataatt ttgcaatgct
     1381 cagtggtgcc agaaaatctt tttccattcc acagggacat attgtaagcc catcaggctt
     1441 tgcaaatctg acgcaatctt ggcaaccaac ttatttgacg caatcccaat gctgcaggta
     1501 agccctgttt gtgtaaaaac atcagtttta atattacgtg caatgttttc cacattccca
     1561 aacagtttca gtgtccctgt acaatctaaa aatgcttcgt caatgcttat ctgttcaata
     1621 cagggggaat agcgctcaaa aattttcata attatgtgcg atagttcaac gtatcggtca
     1681 aaccgtgggg gcaaaaagac agcccctggg catagtttat atgcctgtga aataggcatt
     1741 cctgaatgaa tgccaaacct acgtgcctca tagcttgcag ccgaaactac accgcggccc
     1801 cttccacctt taggatcagc cccaaccaca acaggcttcc cctttaacga aggattatcc
     1861 acctgctcaa ccgaagtaaa gaaggcatcc atgtccacat gaagaattat tcgatctttc
     1921 ataaaaataa ctatacggat atatagtact actgtcaagg agaaattaaa aaaattcttg
     1981 acaatcgtat tatttactgt aaattttacg actatgagcg aaaatataca gtatatttat
     2041 cgaagtctta gcaccttatt acccaaagaa actgaaagaa agcttgtgtt aatcactggt
     2101 gcacgtcaga caggcaaaac tactttagca aaaaaggtat atcagcatct tcgttacata
     2161 aattttgact caccagaaat caggcaaaca gtcaaaaaca tttccacaga agactgggct
     2221 aaggacatag gcactgctat acttgatgaa gcacaaaaag aaccccttgt ttttgaaaaa
     2281 attaaatata gctacgatga gcaatctctt acattttcag ttatattagg ttcaagtcag
     2341 atacttttat taaaaaaaat cagagagtca cttgcaggta gaatatggat atatgagtta
     2401 tggccattga tgcaatctga acttgtaaat atcacttcat ggaatgtccc actttttata
     2461 aagattctgg aatcagatac tcttgatgct ctattacaac agcaaccaac agttatcgtt
     2521 ggccagcaaa tgagcaactg caaaaaagct gaaaactata tactacagtg gggtgggatg
     2581 ccagcactca tccatatata tcaaaacaac gaaaagcatc agtggttgaa aaattatatc
     2641 tatacctatt tagaacgcga tctctctgat ctggctcggt tacaggatct tgaaccattc
     2701 gaaaagtttc aaaaactatc agcactgcga tgttcctcat tgttaaatta ttcagaatta
     2761 tcaagagacg ctggcataag tgtagatacc gcccgcagat acttagaata tttaaaacta
     2821 tcatatcagg ttatacttct ccagccatat atggaaaata tcacaagttc aacggttaaa
     2881 acacctaaaa tatactggat tgacaatgga attatgcgac agctaacagg atatacaagc
     2941 gatacagtaa cgggacaact atatgaaaac catgttattt ctgaaatgca caaatgtatt
     3001 aaaacattgc aaaagccagc atcactttat ttttacagaa ccagatcagg attagaagta
     3061 gatgttgtta ttgaaactgc aaagggatta atcttctgtg aaattaagaa tagggaagaa
     3121 attgtcaaaa gtgatttcac tgccatcaaa tctattgctc aaaaacttgg gaaaaagttt
     3181 cttggtggcc ttatcatcta ccgtggagag cgtttatatc agctttctca tccttcaatg
     3241 tgggctattc cttcatggcg gttatttaca taaaattaaa agcccatctc ttgtcgaatg
     3301 agatgggctt attacggtat ttttatacaa cttattattc ggttactctt acttttaatc
     3361 gtacaggttg tgattgtagc tttttgcctt ttaattctga ttgaatatac acagttatta
     3421 catatacacc tggtttctgg taatagtatt tagcagatac tgagccagat aatatcttgc
     3481 ggaaatcagg tgaaccttct ttgccagtgg ttgaaatact taaactttct cgttcaatca
     3541 tctcgctttt aacggtgcca tcaccccaat ctataacagc cttaatattt tctgattcag
     3601 catcaactga tactatatta atctttaact gatagccatc agtaagtatc tgttttaacg
     3661 aagcatttgg tccttcaatt gaaatctttg gcgttgatgg atcaagccct tgctgtacaa
     3721 taacactata ttctacctga gtaaattcat tgggagatgc tccagtagct ttcaatataa
     3781 gtttatgttt accaggttca ctggtaacaa agtgatgaat cacctggtat tgcttagtat
     3841 tgtacacatc ggtatctatt agtgtaatgg ttttccccat ctgtgtgatg agattgcctt
     3901 ttgaatcagt agtttctaca tactcctgta acactaaatt aacgtgagtt agattagtat
     3961 caggatcact tactgttgcc aggaactgta tctcctgatt tgttccagca ggatttggag
     4021 taattgatac attgctaatt tttggagcaa ttgaacccac gtgcccaaaa aatcgaggtg
     4081 gattttgcat gctgtaaatt atatttccag ctttatcata taccttaaat gttccgctat
     4141 acgtatcgcc aggagttaat ccagttaatt tgtagcttgt ggtagtatac tcagtaacct
     4201 caatggtctt tactggcctt tccatctgta taacatattt gccaaattct gcttctttat
     4261 accacgccgg ccacgatatg gttataaatc ctattcctgg ttcaaccttt ggccagataa
     4321 ctggagcatt ggcaatattc aatttttgcc agtctttttg aagagtatag acttcatacg
     4381 tcgttgtatt gtacatatcc ttagcataaa tggtaaatgt atgcacacca tatgcctgtc
     4441 ctgtcatatc aaacgttttc ctaacatgtg gtttattatt actgttaagc acagcagttg
     4501 caaaaggtgt tgttgttgaa gtcccatcag gtttagtatg tttaatgtaa tactgtgtac
     4561 ctactatatc aaattcacaa tatacttcaa gtgcatttac cactcgcgta acctgagcat
     4621 tgcttacaac aggtggattt gtatttgcct ggcataccac acgtaatgta ttcacatcat
     4681 ctgcaaagga tgtcatgtta tctcattatt gcacaacgat gtaaccatgg gcttacttcg
     4741 ttttcttctt caccggctgt tttggttgtg gcttttttac tccttcagca acaccaagat
     4801 ttttatcaac aattgctttt gttgctgaca gtacttcggc actttcaacc ctgataagtt
     4861 tcaggaag
//
LOCUS       DAOSZP010000103         4859 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_14481_length_4859_cov_3.633847, whole genome shotgun sequence.
ACCESSION   DAOSZP010000103 DAOSZP010000000
VERSION     DAOSZP010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4859)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4859)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4859
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14481_length_4859_cov_3.633847"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="PK348_03320"
     CDS             <1..262
                     /locus_tag="PK348_03320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65276.1"
                     /translation="QASKKEREEILFFPCLRKERCSQASKKEREEILFFPCLRKERCS
                     QASKKERKEILFFPCLHKEIYIFVSKKERVNIIVYLLKKKYY"
     gene            complement(282..980)
                     /gene="tsaB"
                     /locus_tag="PK348_03325"
     CDS             complement(282..980)
                     /gene="tsaB"
                     /locus_tag="PK348_03325"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HXK65277.1"
                     /translation="MNILLLDTATNIEIVALRYNGVVSDKTIQTHHSHSVTLFDTIDS
                     ACKELSTTIHSIQCIGVGIGPGSFTGIRIAVTTARMLAQILQIPLVGIPTQLLFASAI
                     VHSNAHCIVAFDAKKGRVFAARYHITNNSIPQEIVPPGDYTVEQLFNVQNNYDIIAVG
                     DGSIKYQEQMKQLYNTIQFVPDFIPQADRMIHLVESLYAQNPSNYADYRKVLPYYSRK
                     SDAEIMKELREKED"
     gene            complement(961..1389)
                     /gene="tsaE"
                     /locus_tag="PK348_03330"
     CDS             complement(961..1389)
                     /gene="tsaE"
                     /locus_tag="PK348_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013976153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HXK65278.1"
                     /translation="MTKTTYITQSQEETLALGIELGKHAKPGDIFALIGNLGTGKTIL
                     AKGIAKGLGIEEEITSPTFTLLEVYESTLPLYHFDLYRISDDAELENLFFEEYWFGDG
                     VSVIEWADRALKRLPQDIYIITLEYIDAKRRNITIEHPSS"
     gene            complement(1382..2401)
                     /locus_tag="PK348_03335"
     CDS             complement(1382..2401)
                     /locus_tag="PK348_03335"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HXK65279.1"
                     /translation="MPCLHYLYSPKVFQGFFMVNENNITLTVPEEYAGTRIDVFIYNA
                     LEEELSRSFIQKLLKNNHITVDGMPVKPNYKVKSDQQIHIIIPQPEKSTIEPEDIPLH
                     ILYEDNDIAIIHKPAGMVVHPGAGNSQHTLVNALLYHFKGLSSIGGVERPGIVHRLDK
                     DTEGIMVIAKNDTAHNVLTKSFQSRNVIKKYEAIVTGKPSTPTDTINKAIERHPKYGH
                     KMTVRDDGKEAITHYTLREVWHTPQGVFSHLSIQIFTGRTHQIRVHLSSIGLPIVGDK
                     LYSKKWEKYNVPFMMLASTYLAFEHPVTGKHLQFSIDLPQHMKDFIKKINTMAFEHIK
                     TEYYD"
     gene            2502..2912
                     /locus_tag="PK348_03340"
     CDS             2502..2912
                     /locus_tag="PK348_03340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65280.1"
                     /translation="MNDLLYKSFMTVVLSTWNHVVRKPYHVAAFMAVWYYIELLYMMN
                     IAIFFYPPMLISFIGIILGIGLSIHILKLYIGNTINVTIHVFVMDVHIAYSAGLTIAA
                     ILSGATWYAVLILIVRDIIAVIELLLVYTMTKEE"
     gene            3218..3685
                     /locus_tag="PK348_03345"
     CDS             3218..3685
                     /locus_tag="PK348_03345"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXK65281.1"
                     /translation="MYDIREATLSDIDDIVTIRMKMLFELGKLETEDGTRKLRSLTRD
                     FFIRKFQTGEFKVFCALKDETIIATTGIQFLERPPLYENPHGIEAYIMNVYTEPEYRG
                     KGVASKLLEKVIEHAKERKAGRILLHAIGKDKRIYEKAGFVSTTDEMQLVLQY"
     gene            3744..>4859
                     /locus_tag="PK348_03350"
     CDS             3744..>4859
                     /locus_tag="PK348_03350"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HXK65282.1"
                     /translation="MNILLNILIIFSISIAVIYVCNRLKIPTIIGLLITGIVVGLYGL
                     QIIKSTTDVELLAEIGIITLLFTIGLELSFDEIASLKRAIVVGVLQVSITILIVTGIM
                     LVWTSFSQAIFFGFIVSLSSTAVVLKVIQELALSESPHGRISLAILIVQDIAIVPLML
                     FIPLLAGVSKNSIPIEMFKLIVKGALIVVVVIVLARYVVPRLLFEIAKTKSRELFVIT
                     IVTICLGVTLATYSMGMSVELGAFLAGIIIAASEYSGQTFSSMMPFRDLFTSFFFISI
                     GMLLNPVIVVSNIVTIANFSVLILSIKIIIIATIVLALGYPLRIAFLTGFALPQIGEF
                     SFILAQIGLSYALLSQEEYQLFLSITIFAMGITPYLIK"
BASE COUNT         1434 a          771 c          965 g         1689 t
ORIGIN      
        1 tcaagcgtca aaaaaagaaa gagaagagat attatttttt ccttgcttgc gcaaggaaag
       61 atgtagtcaa gcgtcaaaaa aagaaagaga agagatatta ttttttcctt gcttgcgcaa
      121 ggaaaggtgc agtcaagcct caaaaaaaga aagaaaagag atattatttt ttccttgctt
      181 acataaggaa atatacatat ttgtgtctaa aaaagaaaga gttaatataa tagtttattt
      241 gctgaaaaaa aaatattatt aaatgctaga aaaaagtaga tttaatcttc tttctctcgt
      301 aattctttca tgatttcagc atctgatttc cgtgaataat agggcaatac tttacgatag
      361 tcggcatagt tagatggatt ttgagcatat aatgattcca caaggtgtat cattctatct
      421 gcttgaggga taaaatcagg tacaaattgt atagtattat ataactgctt catttgctcc
      481 tggtacttta tactgccatc gccaactgca ataatatcat aattgttttg gacattaaaa
      541 agctgctcta cggtgtagtc acccggtggt actatctcct gtgggatgga attatttgtt
      601 atgtggtaac gtgcggcaaa gacccttcct tttttggcat caaatgctac gatgcaatgt
      661 gcattgctat gaactatcgc cgaagcaaaa agtaactggg ttggtatgcc taccagtggt
      721 atctgtaaga tttgtgccag catgcgcgcg gtggtaactg ctattctgat tccagtgaat
      781 gaacctggtc ctatgccaac acctatacac tgaatgctgt gtattgtggt gcttaactct
      841 ttgcatgcac tgtcaatagt gtcaaatagg gtaactgaat gtgaatggtg cgtttgtatg
      901 gttttatctg atactacacc attgtatctc agcgctacga tttcaatatt tgttgctgtg
      961 tcaagaagaa ggatgttcaa tggtaatgtt cctccttttt gcatctatgt attccagtgt
     1021 aatgatgtat atgtcctgtg gaagccgttt caatgctctg tcggcccatt caatgaccga
     1081 aacaccatca ccaaaccagt attcttcaaa aaaaagattt tcaagttcag catcgtcaga
     1141 gattcggtaa agatcaaagt ggtataaggg tagtgttgat tcataaacct caagcaaggt
     1201 aaatgttggg ctggtgattt cttcttcaat tcctaaacct tttgcaatgc ctttggcaag
     1261 gatagtcttt cctgttccta aattcccaat aagtgcaaag atatcgccgg gttttgcatg
     1321 tttacccagt tcaatgccta atgctaatgt ttcttcctgt gattgtgtta tgtatgtagt
     1381 tttagtcata atattctgtt tttatgtgtt caaatgccat tgtattaatc ttttttataa
     1441 aatccttcat gtgctgtggc aagtcaatag aaaattgtag atgctttcct gtaacaggat
     1501 gttcaaatgc taaataggtt gaagcaagca tcatgaaagg aacattatat ttttcccatt
     1561 ttttggaata tagcttgtcg ccaacgatgg gaagccctat cgatgataaa tgaacccgta
     1621 tttgatgtgt ccttcctgta aatatctgta tgctcaaatg tgaaaatacg ccttgcggtg
     1681 tgtgccatac ttcgcgtagg gtatagtgag ttattgcttc tttaccatca tcgcgtacag
     1741 tcattttatg gccatatttg gggtggcgtt ctattgcttt attgattgta tcggttggtg
     1801 tggatggttt gccggtaact atcgcctcat attttttgat aacattcctg ctctggaatg
     1861 attttgtaag tacattgtgt gcagtatcat ttttagctat gaccatgatg ccttctgtgt
     1921 ctttatcaag cctgtggaca atacctggcc tttcaacacc accaattgaa gaaaggccct
     1981 taaagtgata tagcaaagca ttcaccagcg tgtgctgact gttgcctgca ccaggatgca
     2041 ctaccatccc tgcaggttta tgtattatgg caatatcatt gtcttcatac aaaatatgta
     2101 aagggatatc ttcaggttca atagttgatt tttcaggctg tgggatgata atatgtattt
     2161 gttggtctga tttaaccttg taatttggtt ttacgggcat tccatctact gtaatatgat
     2221 tgttttttaa aagtttttgt ataaagcttc gcgatagttc ctcttccagt gcattgtata
     2281 taaatacatc aatgcgggta ccagcatatt cttcggggac tgttagtgtt atattgtttt
     2341 catttaccat aaaaaagcct tgaaatactt taggactgta aagataatgc aaacatggca
     2401 tcgtttattc ccccagatag cagggaagag tgtacgattg gtgcaataaa atgaaacgtt
     2461 ttgcttcatt tagtcaaatc atttttacat gaggcataca ggtgaatgat ctattgtata
     2521 aatcatttat gacggttgtt ttatctacat ggaatcatgt agtacgtaaa ccttatcacg
     2581 tagcagcttt tatggctgta tggtactaca tagaactcct gtatatgatg aatatagcaa
     2641 tattttttta tccgccaatg ttaatttcgt ttattggaat tatactgggt attggcttaa
     2701 gcatacatat actgaagcta tatattggca atactattaa tgttacaatc catgtatttg
     2761 tgatggatgt ccacatagct tattcagcag gtttgacaat agcagctata ttatcaggtg
     2821 ctacctggta tgctgtactt atactaatag tacgtgatat tatagcagtt atagaactgc
     2881 ttctggtata tacaatgacc aaagaagaat gattaatgtt aatatgtaac tgatttttta
     2941 aaaaatgcta gtgcaaggtt ttgccatgca ccgatgcatg gcaaattagg atgcgggcga
     3001 ggacctgaat ggaggctatt ccgtagcccg caatgaatag aataagttag taagattaac
     3061 aaaaaggcat ttttctttgg cactttcttt tgttgctcgt ttgtgacaaa agaaagtgaa
     3121 aaacaacgga tgtaacatac tttatgattt gacgaaaaaa ataaatctca tttgaatggt
     3181 tgttatgttg agatttgtgt aatggtggag gttttttatg tacgatattc gcgaagctac
     3241 tctgagtgat attgatgata ttgtcaccat acgtatgaag atgctgtttg agttgggtaa
     3301 gcttgaaact gaagatggca cccggaagct gaggtcatta acaagggatt tctttatacg
     3361 taaatttcag acaggtgaat ttaaagtttt ttgtgcatta aaggatgaaa caattattgc
     3421 tacaactggc attcagtttt tagaaagacc accattatat gaaaatcctc atggtataga
     3481 agcatatata atgaatgttt ataccgaacc tgagtacagg ggaaaaggcg ttgcatcaaa
     3541 attgctggaa aaagttattg aacatgctaa agaaagaaag gcaggaagaa tattgcttca
     3601 tgctattgga aaagacaaac gtatctatga aaaggctggt tttgtatcaa caactgatga
     3661 gatgcaatta gtgctgcaat actgacgtaa aaatatctat gtagtaaaat atccaattat
     3721 aaaattttac cacagtatca ggtatgaaca tccttttaaa catattaata atattttcaa
     3781 tatcaattgc agtaatatat gtatgcaatc gcttgaagat tcccaccatc ataggattgc
     3841 ttattactgg tattgttgtt gggctgtatg gacttcaaat cattaaatcc actacagatg
     3901 ttgaactatt agctgagata ggaattatta ccttactttt cactatagga ttagaattat
     3961 catttgatga aattgcatca ctcaaacgcg caatagtagt aggtgtattg caggttagca
     4021 taacaattct tatagttact ggtataatgc tggtgtggac ttctttttcc caggctattt
     4081 tctttggatt tattgtttca ttgagcagta cagcggtagt gttaaaggta attcaggaat
     4141 tggcattatc tgaaagccca catggcagga tatctctggc aattcttatt gttcaggata
     4201 ttgcaatagt accgttaatg ttatttattc cattattggc tggtgtttct aagaattcaa
     4261 ttcccattga gatgttcaaa cttattgtta aaggtgcttt gatagttgtt gttgttattg
     4321 tccttgcacg gtatgtagtc cccagattgt tatttgagat agctaaaact aaaagtcgtg
     4381 aattatttgt tattacaatt gtaacaattt gcctgggtgt tacgttagca acatattcaa
     4441 tggggatgtc tgtagaatta ggggcctttc tggcaggaat cattattgct gcttctgaat
     4501 acagtggcca aacgtttagt tcaatgatgc cgttcaggga tttatttaca agttttttct
     4561 ttatttcaat agggatgtta cttaatcctg tcattgtggt ttctaatata gtaactatcg
     4621 ccaatttttc agttttgata cttagtatca agattattat tatagcaaca attgtacttg
     4681 cacttggata cccattacgg atagcttttt taactggttt tgctttacca cagattggtg
     4741 aattttcctt cattttagct caaattgggt tgtcgtacgc attattgtcc caggaagaat
     4801 accagttgtt tctttcaata actatatttg caatgggcat aacaccgtat ttgattaaa
//
LOCUS       DAOSZP010000104         4771 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_14807_length_4771_cov_3.117684, whole genome shotgun sequence.
ACCESSION   DAOSZP010000104 DAOSZP010000000
VERSION     DAOSZP010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4771)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4771)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4771
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14807_length_4771_cov_3.117684"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..810)
                     /locus_tag="PK348_03355"
     CDS             complement(<1..810)
                     /locus_tag="PK348_03355"
                     /inference="COORDINATES: protein motif:HMM:NF024571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXK65283.1"
                     /translation="MWIERKIESLVYQAFEQFPALLITGLRQVGKTSLLRKMFPDASY
                     ITLDLPANQLAAASNSLEFIQRFARPVIIDEVQYVPQLFQGLKVAIDQQRRNSQFILT
                     GSQNFSFMQGVSESLSGRCAMLQLYPLSFYEVSNYTSISQNEFIFLGGYPELYTSPKN
                     YNLWYSSYVATYLERDVRNVLNVTDLREFNLFLRSCALRISNLLSYTDLARDIGISVN
                     TAKKWLSVLTTLGAVYLVEPYFANRGKRIIKSPKIYFTDTGLAAFLCGFEHA"
     gene            complement(851..1186)
                     /locus_tag="PK348_03360"
     CDS             complement(851..1186)
                     /locus_tag="PK348_03360"
                     /inference="COORDINATES: protein motif:HMM:NF017902.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HXK65284.1"
                     /translation="MSDIEDIKQKAYELANSIAEHPVATRYFSLLQQMNEDEEALQLL
                     SRLVEHGKKLNQKMESGEINIENDPVSVELREALIEKPLVKEFVSAQNEYLGLIKEIM
                     EILHHRLNP"
     gene            complement(1186..2049)
                     /locus_tag="PK348_03365"
     CDS             complement(1186..2049)
                     /locus_tag="PK348_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012161504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HXK65285.1"
                     /translation="MKRFFLLSIVLLTAGMMCCSHQSQAQCTLAPGLHTIALPDAVIN
                     PGKTIPIDIIISDSNCKKNILVLPGWNFSRKRWIEETSLRQEATKRGYNLILPEMSVT
                     IYESQYYPETKRKWAKTPGSQWIKEICIPELQKRGLLLSNQFNAVMGLSTGGRGAVLT
                     ALYNKGIFKAAASLSGDFDQTRMKHDKLMTAVYGPYEYYKNRWENVDNPFAMIAEWYI
                     PLYLGHGTRDTVVPYEQTKIFYEALKTAKPDITITFNDCACGHDFIYWNSEVVPILDF
                     FDTINKKAGNK"
     gene            complement(2046..3188)
                     /locus_tag="PK348_03370"
     CDS             complement(2046..3188)
                     /locus_tag="PK348_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65286.1"
                     /translation="MAKIKSVSLLFVISCIISCGLYNNSSARTQSPFAATPIDTKLVE
                     ATRFLAGKPLSPSSLLFPLSTDKRYLAYVDELRKSWARLQQKNLDEIAQWKKQNLPAR
                     YNKTIFYPFSGPDILNAIAFFPDGDEYIMFGLEPCGNIPDPHAVSKERVFSGLWGLRA
                     SLNEILNMNFFKTIDMQKEVSTNEFNSMISIIMWFLGQLDYEVINAHKIWIDDNSLIT
                     TTPSVMKQNKYIPGAEIFFKDNAGYIKRVRYFQINVIDNSLSYYTNFIPHLESYNRYT
                     TIIKSASYLMHNDNKFTKIRDLTLQHSDLILQDDSGVALKFFSPYQWKLTFFGVYTKP
                     VPLFAHRYQPELKEKFDTLSKGPLPFSYGYNSGIGNSNLMLAERVQ"
     gene            complement(3355..4683)
                     /gene="larA"
                     /locus_tag="PK348_03375"
     CDS             complement(3355..4683)
                     /gene="larA"
                     /locus_tag="PK348_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HXK65287.1"
                     /translation="MKIDLPWGASYISLDLPASWDVIIPRQQEHKLSPKKQNEKDIVA
                     RALANPFAAKPIQKHVLKNKKVVIVVDDNTRPTPAYKFFDIVLDALKKAGCTTKHNAI
                     VIPALGIHTPMKKEEMEEKIGKKNIGQVQWENHDAFDKNKNAYFGKTSRGVEVYLNHH
                     LKDADFIILIGLVEPHLWAGFGGGLKNILPGVAYAETIGNHHAIIAEPPYRFNRVAVD
                     AEHNSFRLDLEETLNMIKAEVFCINVVLDSNKDIIACFAGHPIHCHREAVAFTKTICG
                     LPLHKQVDGIITNSAPMDINFKQSMKCVGNTLPALRPKGIVMGFLRAQRGLDDIPLPD
                     KSTPLWLTRTILRTLGPARVLGFLEKVKPGLDVEQKFLTYYSMQLIRAHNLYFYVPSL
                     SDTEVKALGFFERFNEPQEVIERGLKHLGKNATVAVFKEGGATFPLLSVK"
BASE COUNT         1383 a          923 c          916 g         1549 t
ORIGIN      
        1 tgcatgttca aagccgcata aaaaagcagc caatcctgta tcagtaaaat aaattttggg
       61 tgattttatt attctttttc ctctattagc aaagtatggt tcaactaaat atactgcacc
      121 taacgtagtt aatacagata accatttttt tgcggtatta accgatatcc ctatgtctcg
      181 cgctaaatca gtatatgaaa gtaaattaga aattcttaat gcacaggaac gcaaaaatag
      241 attaaattcg cgtaaatccg ttacattaag aacattgcgc acgtctcttt ctaagtatgt
      301 tgcaacatac gatgaatacc atagattata atttttaggg gatgtatata attcaggata
      361 gccacctaaa aagataaatt cattctgtga tatactggta taattggaaa cctcataaaa
      421 tgaaagcgga taaagttgta acattgcaca tctgcctgat aaactttctg aaacaccctg
      481 catgaatgaa aaattttgtg aacctgttag gataaattga ctattcctcc tctgttgatc
      541 tatggcaact tttagtccct ggaaaagctg gggtacatac tgtacttcat caataataac
      601 gggcctggca aacctttgaa taaactcaag cgagtttgag gctgctgcaa gctggtttgc
      661 tggcagatca agggtaatgt aagaagcatc aggaaacatt tttcttagaa gggatgtttt
      721 tcctacctgt cgtaatcctg ttataagcag tgcgggaaat tgttcaaaag cctggtaaac
      781 aagggattct atttttcttt caatccacat gcagatattt tgcactaaca atgcaaaaag
      841 tcaagcctaa ttatggattt aaacggtgat gcaggatttc cattatttcc ttgataagcc
      901 ctaaatattc attctgtgcg ctaacaaact cttttaccag tggcttttct attaatgctt
      961 cacgaagctc aactgacaca ggatcgtttt caatgtttat ttcacctgat tccattttct
     1021 gatttaactt tttaccatgt tcaaccaggc gcgatagtaa ctgtaaagct tcttcatcct
     1081 cattcatctg ttgtaaaagc gaaaaatatc gtgttgccac cgggtgctct gcaatgctat
     1141 tggctaactc atatgctttt tgctttatat cttcaatgtc gctcattatt tatttcctgc
     1201 ttttttgttg atagtatcaa aaaaatctaa tatgggtact acttcactgt tccagtagat
     1261 aaaatcgtgc ccgcatgcac aatcattaaa tgtaatagta atatctggtt ttgcagtttt
     1321 caatgcctca taaaaaattt ttgtctgctc atagggaaca acagtatccc ttgtgccatg
     1381 accaagataa agaggaatgt accattcagc tatcatagca aaagggttat caacattttc
     1441 ccatcgattt ttatagtatt catagggtcc atacactgct gtcatcaatt tgtcatgctt
     1501 catgcgtgtt tgatcaaaat caccggaaag ggaagcagca gctttgaaaa tgcctttatt
     1561 gtacaaagcg gtaagcactg cacctcttcc accagtggat agccccataa cagcattaaa
     1621 ctggttggat aaaagtaagc ctcgtttctg tagctccggg atacatattt cttttatcca
     1681 ttgagatccc ggtgtttttg cccatttgcg ctttgtttca ggatagtatt gtgattcgta
     1741 gatagtcacc gacatctcag gcaggataag gttgtatcct cgctttgtag cttcctgtcg
     1801 cagtgatgtt tcttctatcc agcgtttgcg gctgaaattc catccaggca acaccaggat
     1861 gtttttttta cagttactat cgcttatgat aatatcaatg ggaattgttt tacccgggtt
     1921 tataactgca tcaggcaatg caatggtatg tagccccggc gcaagcgtac actgtgcctg
     1981 tgactgatgg gaacagcaca tcatgccagc tgtaagcagt actatggata aaagaaaaaa
     2041 acgtttcatt gaacgcgctc tgccagcatc agattggaat tgccaatccc actattatat
     2101 ccatatgaaa aaggcagtgg tcctttagac agggtgtcaa acttttcttt caattctggc
     2161 tgatagcgat gagcaaatag tggtaccggt ttggtgtaga ctccaaaaaa tgtaagtttc
     2221 cactgatagg gactgaaaaa cttaagtgcc acaccggaat catcctgcaa aataaggtca
     2281 ctgtgttgca gggtaaggtc gcgaatcttg gtaaatttat tgtcattatg catgagatat
     2341 gacgctgatt ttataatggt tgtatagcga ttgtaggatt ctaaatgcgg aataaaattg
     2401 gtgtagtacg atagtgaatt gtcaataaca ttgatctgga aataccgcac tcgtttgatg
     2461 taaccggcgt tgtccttgaa aaaaatctct gctccaggta tgtatttgtt ttgcttcata
     2521 acagatggcg ttgttgttat gaggctattg tcatcaatcc agattttgtg cgcattaatc
     2581 acttcataat caagctgtcc caaaaaccac atgatgatgc ttatcatact gttgaactca
     2641 ttggtgctaa cttctttctg catatcaatc gttttaaaaa agttcatgtt aaggatttca
     2701 ttaagtgatg cacgcaatcc ccataatcct gaaaatacac gttctttgct taccgcgtgg
     2761 ggatcaggaa tattgccgca tggttcaagt ccaaacatga tatattcatc gccgtcaggg
     2821 aaaaaagcga tggcgtttag tatatcaggg ccactgaatg gataaaagat agttttattg
     2881 tagcgtgcag gaaggttctg ctttttccat tgtgctattt catctaagtt tttctgctgc
     2941 agccgtgccc agcttttcct taattcatcc acataggcaa ggtaacgttt atcagtacta
     3001 agcggaaaaa gcaaagatga aggtgataac ggtttacctg ctaaaaaccg tgtggcttcc
     3061 acgagtttgg tatcaatggg tgttgcagca aacggtgact gtgtgcgtgc tgatgaattg
     3121 ttatacaaac cacatgatat gatgcaactt attacaaata atagagaaac actttttatt
     3181 tttgccatag atatcccttc tggattaata taatatttta gttgcatctg tatgtatgca
     3241 ggttattatt tatgtcaatg attttattta aaatacagta catactatag cgtctttgat
     3301 atcaggcgta tacacgttca tctgatgcac aatgaacaat caagtttttg ataattactt
     3361 taccgataac aaaggaaaag ttgctccacc ttccttaaac acagctactg ttgcattttt
     3421 gcctaagtgt ttaagtcctc gttcaatcac ttcctgaggt tcgttaaatc gttcaaaaaa
     3481 tcccagtgcc ttaacctcag tatctgatag tgaaggtaca taaaaataga gattgtgtgc
     3541 acgtataagc tgcattgaat aataggttaa aaacttctgc tcaacatcaa ggccaggttt
     3601 aactttttca aggaatccaa gtactcgtgc ggggcctaat gttcgcaaaa ttgtacgtgt
     3661 taaccacagt ggtgtggatt tgtcagggag tggaatatca tcaagccctc tctgtgcacg
     3721 caaaaatccc attacaatgc ctttgggccg caaggcaggc agggtattcc ctacacattt
     3781 catgctctgc ttaaaattaa tgtccattgg tgctgaattg gttataattc catccacctg
     3841 tttatgtaat ggcaatccgc atattgtttt tgtgaaagca actgcttccc tgtgacagtg
     3901 aatggggtga cctgcaaagc aggcaatgat atccttatta ctatcgagaa caacattaat
     3961 acaaaaaact tcagccttaa tcatattgag tgtttcttcc agatcaagtc tgaatgagtt
     4021 gtgttctgca tcaacggcaa ctctgttaaa acggtacggt ggttcagcaa tgattgcatg
     4081 atgattccca atggtttcag cgtacgcaac accaggcagt atgttcttaa gcccaccgcc
     4141 aaatcctgcc cacaggtgcg gctcaacaag acctataaga atgataaaat cagcatcttt
     4201 tagatgatgg ttaagatata cttcaacacc gcgtgaggtt ttaccaaaat aagcattttt
     4261 gttcttgtca aacgcatcgt ggttttccca ttgcacctgt ccaatatttt ttttgccaat
     4321 cttttcttcc atttcctctt ttttcattgg agtgtgaata cccagtgcag gaataacaat
     4381 agcattatgt tttgttgtac atcctgcctt tttaagtgca tccagaacaa tgtcaaaaaa
     4441 cttataggca ggagtggggc gggtattgtc atccacgaca atcacaactt ttttattttt
     4501 caaaacgtgt ttttgaatag gttttgctgc aaaagggttg gccaatgctc ttgcaactat
     4561 gtctttttca ttttgttttt tgggcgatag tttatgctcc tgttgcctgg gaataataac
     4621 atcccagcta gcaggaagat ccagggatat gtatgaagca ccccatggta aatctatttt
     4681 catggcaacc tccagtgaat gtgtttttta tttttttaca atactgtttt gtttatgtca
     4741 atattattta ttgttataag gctctgaccc c
//
LOCUS       DAOSZP010000105         4737 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_14921_length_4737_cov_3.610209, whole genome shotgun sequence.
ACCESSION   DAOSZP010000105 DAOSZP010000000
VERSION     DAOSZP010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4737
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14921_length_4737_cov_3.610209"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="PK348_03380"
     CDS             <1..308
                     /locus_tag="PK348_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65288.1"
                     /translation="LWSFKLERMLAIYGTVTGTVSSGLLLLRIVDPEFKTTVALETGV
                     MLMFASPYIILGMLWVSAPVLWQWSLIKTILVFAGMFVVAISITEIQRFLILKKKHA"
     gene            409..1017
                     /locus_tag="PK348_03385"
     CDS             409..1017
                     /locus_tag="PK348_03385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65289.1"
                     /translation="MNILEELKGYFETRLNECKTNQELNKHQIAAYTAYLEKLDAIHD
                     DDYGKVAEAIGEMFLIARAEQRDRYESLKRIKTLFGDREGVKAEQIMIDAVQASSNHT
                     ELYSNISRAKDDAGILIQNTHRALELIVKLITRCVEYHVSCKSRKQKRKAEVVAIWNE
                     LQQCDPEITVDKIMMHKPYRRVIPFDNERLSLILSWLGEVLQ"
     gene            1017..2021
                     /locus_tag="PK348_03390"
     CDS             1017..2021
                     /locus_tag="PK348_03390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65290.1"
                     /translation="MDDTIKQMLSGYQTALAAYKQKLGESHAKLMKAYDIYAKLCKKA
                     ELLNDAMTFYSDKEVSTSMADMSALLVELAQEKQDTSLATIPSVDQVAAAYHIAYEQL
                     PMEMKKTRSVYERIFEIEKQSQNALTFLRTMADEKIFLKLSIMQQLEQLEGKKEEAQR
                     NSNPVMVNYYEKMEAKIPNVMSIAELEYYANLESEIAIYQNWWDILLLNTSVTLLCNA
                     IAGWLLTQSEDDREEVENAYRFIAYFYGIDMDELFAVPRFKDHVVKVISKSVNNSNSM
                     ESAEALISQFKNAIQACMNGRDPVKRGPAKNQTLILWEREAPLQALEEAYKTNVYKTL
                     "
     gene            2076..2885
                     /locus_tag="PK348_03395"
     CDS             2076..2885
                     /locus_tag="PK348_03395"
                     /inference="COORDINATES: protein motif:HMM:NF004825.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXK65291.1"
                     /translation="MNNLQGKVTVITGGASGIGAAIALEFGLHGSSIAILDVDTIALK
                     NQVNFLQRNNIPVIGFQCDITNEKQCTVTIAKIIKQYGGIDILVNNAGITQRSLFIDT
                     NAAVYKKVMDVNFFGALYCTRAAINSIIERKGVIVVISSIAGIAPLYGRTGYSASKHA
                     LHGFFESLRAELKDDGVCIMMVCPGFTRTNLQSRALSGNGTINTLDRAIVGREASPQS
                     VAQAIYKGVIKRKRMLVLTTVGKLSFLIAKYFPRLYEIMMSKSVRKEFIKR"
     gene            complement(2906..3496)
                     /locus_tag="PK348_03400"
     CDS             complement(2906..3496)
                     /locus_tag="PK348_03400"
                     /inference="COORDINATES: protein motif:HMM:NF036525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HXK65292.1"
                     /translation="MRKNNRQQILDIARKLFLEFGYNGISIRNIAKRAKLTTGAIYFH
                     FKNKKQIYSTICLEAIDTLHKLFCEGMAARKTPAQKLISTYDSYLKFYYEYHDYYNIL
                     TEYKAEYDDEPDPHKDEIQKKNVALLELMAGAFTDGVKRGDFKNLDPKMVAFFLSAVA
                     EGMLQFKKLGVMDAAGISDKNFREFMKIIVGQGIQK"
     gene            3648..>4737
                     /locus_tag="PK348_03405"
     CDS             3648..>4737
                     /locus_tag="PK348_03405"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HXK65293.1"
                     /translation="MKKILSSIDQAVLLRAIEQSPASIVITDTNGIIQYVNPKFEAIT
                     GYTFDEAVGKNPRILKSGFQSLEFYQHMWHTILRGEVWDGIFHNKRKDGNFFWEHCVI
                     APVVDEKGNVTHFVAIKEDITEKKEQEEALRKSEEELRKRNEIISKDLEYAKRVQHSI
                     INTTLPQYPGIKIYTQYIPYDYVGGDFFTLKTIDENLCIFMADITGHGVPAALFSTVL
                     KYAVENLWNSRILSPDQFMFKLNQELIDILSGYFFTAFYGVITTNLKGELYLTYTRCG
                     HPYPLLLSNNTISMLYSSGTLLGIYRDIQITSNTIRLSNNDRLFIFTDGLVEMDINNK
                     KDLRFDIIAEAAIKTKDMELQEAVQTLLD"
BASE COUNT         1590 a          730 c          961 g         1456 t
ORIGIN      
        1 gtttgtggag ttttaaatta gaacgcatgc ttgccatata tggaactgta acaggaacag
       61 tgtcatccgg attgttgctt ttgcgtatag ttgatcctga atttaaaaca accgtagcgc
      121 ttgaaacagg tgttatgctc atgtttgcct ctccatatat tattttaggg atgctctggg
      181 ttagtgctcc ggttctatgg cagtggagtt taataaaaac aattcttgtg tttgctggca
      241 tgtttgttgt agctatctcc ataactgaga tacagcgttt tcttatttta aaaaagaaac
      301 atgcctaatt tataaatctt tgtataaact tactttttaa acaatgcgtg aagagtaatg
      361 ttaaaatatt attttctaaa attaaaattt agtaatgaaa gatgagttat gaatatactt
      421 gaagagttaa aaggatattt tgaaacaagg cttaatgaat gcaagaccaa tcaggaatta
      481 aataaacatc aaatagctgc ctatacagca tatttggaaa aattggatgc aatacacgat
      541 gatgactatg gcaaagttgc tgaagcaata ggcgaaatgt ttttaattgc cagggctgaa
      601 caacgtgata gatatgaatc tttgaaacgc attaaaacgc tttttggtga tagggagggt
      661 gtgaaagctg agcaaataat gattgatgcg gtgcaggcat cttcaaacca tacagagctt
      721 tatagtaata tttcaagagc aaaggacgat gctggtatac ttatacaaaa tactcataga
      781 gcattagaat taattgtaaa acttataaca cgatgtgtgg aataccatgt atcctgtaaa
      841 agcagaaaac agaaaagaaa agctgaagtt gtagctatct ggaatgaact gcagcaatgt
      901 gatcctgaaa tcactgttga taaaatcatg atgcataaac catatcgccg ggttattccg
      961 tttgataatg aaagactttc actaattctt tcatggcttg gtgaggtgtt acaataatgg
     1021 atgatacaat taaacagatg ctatcaggtt accagacagc tctggcagca tataagcaaa
     1081 agttaggtga gtcacatgct aaactgatga aagcatatga tatatatgca aagctttgta
     1141 aaaaggctga attgctaaac gatgcaatga ctttttattc tgataaagaa gttagcacat
     1201 caatggctga tatgagtgcg ttacttgttg aacttgcgca ggaaaaacaa gatacatctc
     1261 tggcaacaat accttctgtg gatcaggttg ctgctgctta tcatattgcc tatgaacagc
     1321 ttcctatgga aatgaagaaa acacgttcag tatatgagcg catttttgaa attgagaaac
     1381 aatctcagaa tgccctaacg tttttacgca caatggcaga tgaaaaaatc tttttgaagt
     1441 tgagcataat gcagcaactt gaacaattgg agggaaaaaa ggaagaagcc caaagaaatt
     1501 ccaatcctgt tatggtcaac tactatgaaa agatggaagc aaaaattccc aacgtgatgt
     1561 caatagctga attagaatat tatgcaaatc ttgaaagtga aattgcgata tatcaaaact
     1621 ggtgggatat tttactactc aataccagcg tgacacttct ttgcaatgca atagcaggtt
     1681 ggttattgac acaatctgaa gatgatcgtg aggaggtgga aaatgcgtat cgttttattg
     1741 catacttcta tggtattgat atggatgagc tctttgctgt gccacgcttc aaggaccatg
     1801 ttgtgaaagt aatatcaaag tcggttaaca attcaaatag tatggaaagt gctgaagcat
     1861 tgatttcaca atttaaaaat gctatacagg cctgtatgaa tggtcgtgat cctgttaaaa
     1921 gaggtcctgc aaaaaatcag accttgatat tgtgggaacg tgaagctccg ttacaagcac
     1981 ttgaggaagc atataaaacc aacgtatata aaacgttgta atgtatatta ttgtatcatg
     2041 tgcgaaagtt tgtataaaaa taaaaaaggt ttgtcatgaa taatttgcag gggaaagtca
     2101 ccgttatcac tggcggtgca agcggtattg gtgctgccat agcgctggaa tttggattac
     2161 acggttcatc gatagcaatt cttgatgtgg atacaattgc attgaagaat caggttaatt
     2221 ttttgcaacg taataatata cctgtgattg gttttcagtg tgacattacc aatgaaaaac
     2281 aatgcacggt aactatcgcc aaaataataa aacaatacgg tggtattgat atactggtta
     2341 acaacgcggg cataactcag cgtagccttt ttattgatac aaacgctgcg gtgtataaaa
     2401 aagttatgga tgttaatttc tttggcgccc tgtattgtac cagggcagct ataaacagca
     2461 ttattgaacg caagggtgtt attgttgtta tttcaagtat tgctggtata gcgccattat
     2521 atggaaggac aggatacagt gccagcaaac acgctcttca cggattcttt gaaagcttgc
     2581 gagccgagct aaaggatgat ggtgtttgta ttatgatggt atgtcctgga tttactcgta
     2641 ctaatttgca atcacgggct ctttctggaa atggaactat caatacttta gatagggcta
     2701 tagttggcag ggaagcaagt ccacagagtg ttgcacaagc aatttataaa ggggttataa
     2761 agcgcaaaag aatgctagta ctaacaacag tggggaaact cagtttttta atagcaaaat
     2821 attttccacg attgtatgaa ataatgatga gtaaaagtgt aagaaaagaa tttattaaac
     2881 gttgagtaaa gaaaatgtta cagtcctatt tttgtatacc ctgaccaacg ataatcttca
     2941 tgaactccct gaagtttttg tcgctgatac ctgcagcatc catgacacct aattttttaa
     3001 actgaagcat gccttctgca actgcagaaa gaaaaaaggc aaccatcttg gggtctaaat
     3061 tcttaaagtc acctcttttt acaccgtcag taaacgcacc tgccatcaat tcaagtaaag
     3121 ctacattctt tttctggatt tcatctttat gtgggtcagg ttcatcatcg tattcagctt
     3181 tgtattcggt taatatgtta tagtaatcat gatattcata gtaaaattta agatatgagt
     3241 catacgttga gataagtttc tgtgcaggag ttttacgtgc tgccatgcct tcacagaaca
     3301 gtttgtgcag ggtatcaatt gcctcaaggc aaatggtgct ataaatctgc tttttatttt
     3361 taaaatggaa atagattgcc cctgttgtca gttttgcccg tttggcaata tttcgtatgg
     3421 atatgccatt gtagccaaac tccagaaaaa gtttgcgggc aatatcaagg atttgttgac
     3481 ggttattctt tctcatgatg acattgcata agaatgtgac agaaagggtc aagatatttt
     3541 tttaaatttt aaaaggaaaa cgatgatgat atctattgaa aaaatgatgt attgtcatta
     3601 acgtgatatt atatcaacaa ataaatctat tataacataa tattagcatg aaaaagatat
     3661 tatcttccat tgatcaggca gttttgctca gagcaataga acagagccct gcttccattg
     3721 tcataactga cacaaatggt ataattcagt atgttaatcc aaagtttgaa gctatcacag
     3781 gatacacttt tgatgaagct gtgggtaaaa atccacgaat attgaaatca ggatttcaaa
     3841 gtttagaatt ttatcaacac atgtggcaca ccatactgcg tggtgaggtg tgggatggaa
     3901 tttttcataa taagcgaaag gatggtaact ttttctggga acactgtgta atagcgcctg
     3961 ttgttgatga aaaaggaaat gtaacgcatt ttgttgctat taaagaagat ataactgaaa
     4021 agaaagagca ggaagaggcc ttgcgtaaaa gtgaagaaga attgcgcaaa cgaaatgaaa
     4081 ttataagcaa ggatttagaa tatgcaaagc gcgtccagca ttcaataata aataccacac
     4141 tgcctcaata tcctgggata aagatatata cacagtatat tccctacgat tatgttggtg
     4201 gcgatttttt caccctgaaa actattgatg aaaatctttg tatttttatg gctgatatta
     4261 ccggacatgg agtgcctgct gctttatttt caacagtttt aaaatatgct gtagaaaatt
     4321 tatggaatag taggatttta tctcctgatc aatttatgtt taaacttaat caggaactga
     4381 tagatatatt gtcaggatac ttctttaccg cgttttatgg agtcataaca acaaacttaa
     4441 agggtgaatt gtatttaaca tatacccgct gtgggcatcc ctatccacta ttattatcaa
     4501 acaataccat ttcaatgtta tatagctccg gtacattatt aggtatttac cgggatatac
     4561 aaattacaag caatacaata agactttcaa acaatgacag attatttata tttaccgatg
     4621 gccttgtgga aatggatatt aataacaaaa aggatttgcg gtttgatatt attgctgaag
     4681 cagcgataaa gactaaagat atggagttgc aagaagcagt tcaaacactg cttgata
//
LOCUS       DAOSZP010000106         4735 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_14930_length_4735_cov_3.476282, whole genome shotgun sequence.
ACCESSION   DAOSZP010000106 DAOSZP010000000
VERSION     DAOSZP010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4735
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14930_length_4735_cov_3.476282"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="PK348_03410"
     CDS             <1..369
                     /locus_tag="PK348_03410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65294.1"
                     /translation="PQELIVTSLIRAKFAELLASSSQLKPQSSECFLMGLFSLVDAFF
                     DRDKKEIFQQLPLTDDIKNAILKQQGPYAKLYQFITAYEKGNWEAVNRLSEELQLPAE
                     QIAQSYRKAIEWANTFFVHQ"
     gene            416..1228
                     /locus_tag="PK348_03415"
     CDS             416..1228
                     /locus_tag="PK348_03415"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HXK65295.1"
                     /translation="MHTRNNTVIAITCFVIIWAIAHSMHSQAAKKSLQQYTRIVSLSP
                     SITAMIQDLGCEHLLVGVTQYHPPLSRAIPIVGTITNPNSEAIIALQPDIILHSKEDS
                     PTQKIDMLHHAGLRLYALPSADSFETICSNYRTIAGILNIKDYAIHKLSEYKKKRHAL
                     IHKINRSALILLSANPFIAVSRHSYISNIFADAGIDNTLYYSKTRYPIVQLEYLLAQK
                     VDMIIVLSNDILPDTIEKTKNIVHIPYDGLYLYTPRHYCDSLQHVVTVLYGK"
     gene            1329..2288
                     /locus_tag="PK348_03420"
     CDS             1329..2288
                     /locus_tag="PK348_03420"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HXK65296.1"
                     /translation="MKKFSIIISIILVCTGCLLILAIALSTGASHITLLQAITGTDFV
                     AKQIIMLRFLRVLMAFFVGAALAVSGCIFQALLKNPLAEPYTLGVSGGAAFGASIAII
                     TASGAVSVYAFIGGVCTVVVIFFIAKAMQFHPTAIILAGISLGFVLSSGVLLVFAFAK
                     PDTIHKTLMWLSGDLSMAHYEHITLYCMSILVLTTMTLFFHRHLDIISFGTGFATTHG
                     ISTVHIAILFWIASLATSITVAATGIIGFVGLIVPHAVRSIKANHAFLLPVSAIAGGS
                     TLVLADTIGRSIVMPYEIPSGIITGFFGGIFFLILLLTRRDTV"
     gene            2285..3067
                     /locus_tag="PK348_03425"
     CDS             2285..3067
                     /locus_tag="PK348_03425"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXK65297.1"
                     /translation="MSLFEFTNIQAGYRKIPVLDIPRCTINTGDFIGIIGPNGAGKST
                     LLKLLARILAPLSGTIAYKKQNITTIPHTVYATQVSSVTDIERVLPYTVESFIALGRF
                     PHATVTGLKAHDYKAINEAMQYCNVQHLQQKKLTELSSGELQRVNIARALAQSTECIL
                     LDEPVSHLDIHHAIAIMDILRKLNENGSTIIAILHDINIASEYCSRIIGVKDGKIFFD
                     GKPQDCITYQNIEALFDCVCLVYTNPISGKPYVYPVPEHVKQ"
     gene            complement(3080..4639)
                     /locus_tag="PK348_03430"
     CDS             complement(3080..4639)
                     /locus_tag="PK348_03430"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HXK65298.1"
                     /translation="MNLIDAIAAEGRKTANKIRIVVAIILFLVVVSAAPNNPAIVNIS
                     YLITITIFTVISIVNIQSVSSVFYSKIIKFGTVLFEISIPTILKLSHLATAKPHLMIN
                     EGAAFQAYFLFIMLTLFQNDRFLTILTGIVATVEYSVLVIIGIFVMHMPVISGSSEWG
                     HIVIDDEIGKIVMLIGFTAITVAVLRNLQRFAFTALENEKNALTKADQLKEIVNEAKT
                     VNKSLIDVSQNQTEISAVLSDVANDEATMSEELSSMYEEQTSAIELIYKNTIKQTDET
                     KLMKDRIHEFIEIQKNVITVGEQMLNNIVSITDYSKKTEASLNELSHIMDIVTQSGGA
                     INSFLEVIRNITDQINLLSLNASIEAARAGEYGRGFAVVADEIGKLAMATSDNAKEIS
                     SQLARINQDITSSISIMQNTQKALNDMIAIISASKSNINEVRNAIGNQQKAIEDVKRQ
                     ADVLDSLSHEILMAINEQQSSMNESVSSIQKLSDVAQIVADRTMQMNELTIQINAIAQ
                     TLAKVTAAIDN"
BASE COUNT         1418 a          999 c          852 g         1466 t
ORIGIN      
        1 ccacaggaac tcattgtaac atcattaata cgtgcaaaat ttgctgaact tttagccagc
       61 tcatcacagt taaaaccaca atcgtccgaa tgtttcctga tgggcttatt ttcactggtt
      121 gatgcatttt ttgacaggga taaaaaagaa atttttcagc aactacctct tactgatgat
      181 ataaaaaatg caattttaaa gcaacagggg ccctatgcaa agctttatca atttattact
      241 gcctatgaaa aaggtaactg ggaagcagtg aacagattat cagaagagct tcagttacca
      301 gctgagcaga tagcgcaatc ataccgtaaa gcaattgaat gggctaacac attttttgtg
      361 catcagtaga acatattcct tgacagacat ttccagacta ttatcaccat gatacatgca
      421 taccagaaac aacacagtta ttgcaatcac ctgttttgtt ataatctggg cgatagctca
      481 tagcatgcat tcacaggctg ccaaaaaatc cttgcagcag tacacccgca ttgtctcact
      541 gtcgccatcc ataaccgcaa tgatacagga tttaggatgc gaacatttac ttgtaggagt
      601 cacgcaatac caccctccgc tttcaagggc tatacctata gtgggcacta tcaccaatcc
      661 caacagtgaa gcaattattg cattacagcc tgatatcata ctccattcaa aagaagattc
      721 accaacacaa aagattgata tgctccatca tgctggcctt agattgtatg ctctaccttc
      781 ggcagattca tttgaaacta tttgcagtaa ctatcgtaca atagcaggta ttctgaatat
      841 aaaagattat gccattcaca aactttcaga gtataaaaaa aagcgccatg ctcttattca
      901 caaaataaat cgcagtgcac ttattctgct ttcagccaat ccttttattg ctgtctcacg
      961 gcattcatac atcagcaata tctttgccga tgctggcatt gacaatacat tgtactacag
     1021 caaaacgcgc taccctattg tacagttgga gtatttgctt gcgcaaaaag tcgatatgat
     1081 tattgtactg agcaatgaca tcttacccga tacaattgaa aaaacaaaaa acatcgttca
     1141 catcccgtat gatggattat atctgtacac accacgccat tactgtgatt cattgcaaca
     1201 cgtagttact gtgttatatg gtaagtgaaa tatttatggc aaaatttatc tattatgagc
     1261 tatcgcacca atttaaaatg ttgacataat gaagctttga tactgatact tttatccaac
     1321 tgtaagctat gaaaaaattt tctataatca taagtattat actggtctgt acaggctgct
     1381 tactcatact ggcgatagct ctttcgacag gtgcatcgca cataacattg ctacaggcaa
     1441 tcacaggcac cgacttcgtt gcaaaacaaa taattatgct gcgttttctt agagtactca
     1501 tggcattttt cgttggtgct gctctggcag tgtcaggctg catatttcag gcactgttaa
     1561 aaaatccact ggccgaacca tatacactgg gtgtatcagg tggggcagcg tttggtgcaa
     1621 gcattgccat tattactgca tctggcgcag tttcggtata tgcatttatt ggtggtgtgt
     1681 gtactgtggt agtgattttc ttcattgcta aggcaatgca gtttcatccc acagcaatta
     1741 ttttagccgg catatcatta ggctttgtgc tttcatcggg ggtactgctt gtatttgcat
     1801 ttgcaaaacc cgataccata cacaaaacgc tgatgtggct ctctggcgac ctgtcaatgg
     1861 ctcactatga acatatcaca ctgtattgca tgagtatact ggtgcttaca acaatgacac
     1921 ttttttttca tcggcattta gatatcatat catttggtac agggtttgcc acaacccatg
     1981 gcatcagcac agttcatata gcaattcttt tctggatagc atcacttgca acaagcatca
     2041 ccgtggccgc aactggcatc attggctttg ttgggctcat tgtgccgcat gcagtgcgca
     2101 gtataaaagc caaccacgca tttcttttgc cggtatcagc aattgctggc ggcagtacac
     2161 tggtactggc cgacaccatt ggccgaagca tcgtcatgcc ctatgaaatt ccatcaggca
     2221 ttattactgg attctttggc gggatattct tcctgattct tctattaaca cgaagagaca
     2281 cagtatgagt ttatttgaat tcaccaacat acaggctggt tacagaaaaa ttcctgtgct
     2341 cgatattccc cgttgtacca ttaacactgg cgattttatt ggcatcattg gaccaaacgg
     2401 tgcaggtaaa tcaacactgc tgaaactgct tgcacgcatt ttagccccct tatcaggaac
     2461 tatcgcctat aaaaaacaaa atattacaac cataccacac acagtatatg caacacaggt
     2521 atcatcagta actgatattg aacgtgtgct tccctacacg gtggagtcgt ttattgcgtt
     2581 aggcaggttt ccccatgcta cggttacagg tttaaaagca catgattaca aagcaattaa
     2641 tgaggccatg cagtactgta acgtacagca cttacaacaa aaaaagctta ccgagctttc
     2701 ctcaggcgaa ctgcagcgtg ttaacattgc ccgtgcgctg gctcagagta ctgagtgtat
     2761 tctactggat gaaccggtat cgcatttaga tatacatcat gctattgcca tcatggatat
     2821 cctgcgcaag ctcaatgaaa acggcagcac catcattgca atactgcatg atatcaacat
     2881 tgcatctgaa tactgtagta gaataattgg cgttaaagat gggaagatat tttttgatgg
     2941 gaaaccacaa gactgtatta cctatcagaa tattgaagcg ctgtttgact gcgtgtgtct
     3001 tgtgtataca aatccaatct caggaaaacc atacgtgtac cctgtacctg agcacgttaa
     3061 acaataaatc tctgaccttt taattatcaa ttgcagcggt aactttagcc aatgtttgtg
     3121 caattgcgtt aatctgaatt gtcaattcat tcatctgcat ggttctatct gccactatct
     3181 gggctacatc cgatagtttc tgtatggatg acacactctc attcatggat gactgctgtt
     3241 catttattgc catgagtatt tcatgggaaa gtgaatcaag cacatcagcc tgacgtttta
     3301 catcttctat agccttttgc tgattgccta ttgcattacg cacctcattg atgtttgact
     3361 tgctggcact tataatggca atcatgtcat tcaatgcctt ctgcgtattt tgcatgatgg
     3421 aaatactgct ggttatatcc tgattaatgc gtgccagttg cgatgatatt tcctttgcat
     3481 tatccgaggt agccattgcc aatttaccaa tttcatcagc aacaaccgca aaacccctgc
     3541 catattcacc agcccgggct gcttcaatgc ttgcatttaa cgaaagcaaa ttgatctgat
     3601 cagtaatgtt acgaataact tctagaaatg aattaatggc accaccgctt tgagttacta
     3661 tatccatgat atgtgataat tcatttaaac ttgcctctgt tttttttgaa tagtcagtta
     3721 tgctaacaat attattcaac atctgttcgc caacagttat tacattcttc tgtatctcaa
     3781 tgaattcatg gatcctgtct ttcatcaatt ttgtttcatc tgtttgtttg atagtatttt
     3841 tgtaaataag ttcaattgcc gaggtttgtt cttcgtacat tgatgaaagc tcttcactca
     3901 ttgtagcttc atcatttgcc acgtctgaaa gaacagcgct aatttcagtt tgattctggc
     3961 ttacatcaat aagcgacttg tttacagttt tagcttcatt aactatctcc tttaactgat
     4021 cagctttagt tagtgcattt ttttcatttt ccagagcagt aaatgcaaat cgttgtaaat
     4081 tccgtaatac agcaacagtt attgcagtaa agccaatgag cataactatc ttgccaattt
     4141 catcatcaat gacgatatgc ccccattctg aactacctga aataaccggc atgtgcataa
     4201 caaaaatacc aataataacc agtacactgt attcaaccgt agccacgata ccggtgagga
     4261 ttgttaaaaa ccgatcattc tgaaacaacg ttaacatgat aaataaaaag tatgcctgga
     4321 aagctgctcc ttcattaatc atcaggtgtg gctttgcagt agccaaatga gaaagcttga
     4381 gaatagttgg tatggatatt tcaaaaagta ctgtaccaaa tttaataatt tttgagtaaa
     4441 ataccgaaga taccgattga atgttcacaa ttgaaataac tgtaaatata gtaatggtta
     4501 tcagataact tatattaaca attgcaggat tgtttggtgc tgctgataca acaactaaaa
     4561 ataaaataat tgcaacaaca atccgtatct tatttgcagt ttttctgcct tcagcagcaa
     4621 tagcatctat aagattcatt atcaattctc cagaataata tgatcatagt attgggacgt
     4681 aatgagcagc ctcaatgata aaaataatcc taatatgagc agacgctcgt tattt
//
LOCUS       DAOSZP010000107         4706 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_15035_length_4706_cov_3.685659, whole genome shotgun sequence.
ACCESSION   DAOSZP010000107 DAOSZP010000000
VERSION     DAOSZP010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4706
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15035_length_4706_cov_3.685659"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..895)
                     /locus_tag="PK348_03435"
     CDS             complement(<1..895)
                     /locus_tag="PK348_03435"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXK65299.1"
                     /translation="MEQQKKLYTDTNLLIAFGITLVVVMGVTNITPALPAMAQYFAIP
                     YSSVTLVITVFTMPGIVLTPLLGIVADRIGRKIIIIPSLILFGITGVIMFFITDFTWL
                     IVLRFIQGMGVSALGAINATIIGDMFSGKDRTIAFGYNASVLNIGTAAYPAIGGFLCI
                     LGWNYPFLLSALALVVAWFAIFHLHNPEPVTTLSLKRYFHAIANIIKSPYVIGLLSTT
                     VLTFILLYGPIITYFPYIISYRFKGTAATIGLFMSLMSIVTAITSSQLQILTKRYAEK
                     NLILYGFIGLCISFVIAGFAYN"
     gene            1190..3004
                     /locus_tag="PK348_03440"
     CDS             1190..3004
                     /locus_tag="PK348_03440"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HXK65300.1"
                     /translation="MAQFKSESMSAVFQEQAAKLGSKACVSYKKGSQWVDISWADMNT
                     MVHNMAYYLMDYGIKKGDRVAIFSPNRWEWWVASLATTSIGAVSVPIYATNSAEEAQY
                     VLEHSGSSICFVGTEDHADRVLKVIKKCPKLEQLVIFDDLAKKKQNVITFKEALEKGA
                     KKAKPAEFEKRLKAIKRDDVSTIIYTSGTTGNPKGVMLSHYNFYSNAKNIMADMGKFF
                     NTEDVWLSFLPLSHSLEMTCGFYGPIWVGGKTAFAESIEKLLDNFKEVRPTVIISVPR
                     IYEKVHAGILAQVAAAPGLKKAIFNFALNTAKKNLPYVCRDLPRKGLFAIRYNIADKL
                     VFSKLKAAIGMDKMRYAISGGAPLSVSDAEFFIGMGMKILEGFGLTETTPVLTYNRPW
                     FIKPGTVGQAIPETKIKIADDGEILAKGPQIMLGYYKNKAATEEVMTKDGYFKTGDIG
                     VIDEDGFLKITGRIKDIIVTAGGKNISPQNIENSVKTSPYVEQIAVIGDKRKYLSALV
                     IPNFEALGKWAKQKGISFSSNADLIKNDEVNKLIESEIAKYTKQFSRVEQIKKFTLLE
                     AEWTQATGELTPTQKVKRRIIEQKYAKEIEAMYPPDIE"
     gene            3168..4628
                     /locus_tag="PK348_03445"
     CDS             3168..4628
                     /locus_tag="PK348_03445"
                     /inference="COORDINATES: protein motif:HMM:NF006113.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family peptidase"
                     /protein_id="HXK65301.1"
                     /translation="MIKNVLKIVGILILIFIIILLVKAITISPKQVEVKEAIPYKIDS
                     MIVAQHLSDAIQIQTVSNQDQAKTDVKQFEQFHKFLAKTYPALHKKLKKEIVGGYALL
                     YTWKGSDPSLKPMLLMAHQDVVPVEELTKDQWKYPGFSGTIADGFIWGRGALDIKNQL
                     ISIMEAVEFLINKGFVPKRTIYLAFGHDEEVGGEGAKAIAKLLKQRNVQLEYVLDEGG
                     AIMEGMLPGVETPIALVGIAEKGYLTVKLTVKGEGGHSSMPPHHTALGILSKALVDVE
                     DNPFPKNFDSPARQMFEYVAPHMKFPLRLLFANMWCFKPLLKWQFEKSESTNAMIRTT
                     AAVTMAGASEKENVLPTQAWAMINCRILPGETMDSTIAYLKKVIGNDAVTIEATPWSN
                     DPSPVSDIQAGSFTSIATIARQINPDVIVAPYLVLGATDSRHYNALTNQIYRFSPVKM
                     NSEDLKRVHGINERISISDMGKSVKFFIALIEKTHM"
BASE COUNT         1547 a          794 c         1017 g         1348 t
ORIGIN      
        1 aattgtatgc aaaacctgct atgacaaacg atatacaaag ccctataaag ccatacagta
       61 tgagattttt ttctgcatag cgttttgtaa ggatttgtaa ttgcgatgat gtgatggcag
      121 ttactatgga cataagagac atgaaaagac caatagttgc tgctgtccct ttaaaccggt
      181 atgatatgat atagggaaaa taggttataa ttggcccata cagaagtatg aatgtgagca
      241 cagttgttga caggagtcca atgacatagg gactttttat gatattggct attgcatgaa
      301 aatagcgttt taatgataaa gtagtaactg gctcaggatt gtgtaaatgg aatatggcaa
      361 accatgccac aacaagtgct aaggctgata gtaaaaaggg ataattccag cccaggatgc
      421 acaaaaatcc tccaatagca gggtatgcag cagtaccaat atttaacaca ctggcgttgt
      481 atccaaatgc aatggtgcgg tccttcccac tgaacatatc accaatgatg gttgcattaa
      541 ttgctcctaa tgccgacaca cccatgccct gaataaaccg taatacaata agccaggtga
      601 agtcagtaat aaaaaacatg ataactccag taataccaaa tagtatcagt gatggtataa
      661 taattatttt tcttcctatg cggtcagcta caatgcccaa cagaggagtc aacactatcc
      721 ctggcatggt aaaaactgtt ataaccagtg tcactgatga atagggaata gcaaagtact
      781 gtgccattgc agggagtgct ggtgtaatat tggtaactcc cattaccact accagtgtta
      841 taccaaaagc aatgagcaga tttgtatcag tgtagagttt tttttgctgt tccatggcaa
      901 tagtattgtt gtcattttcc cttcatagat agtataaaca taacattaat agtacctcat
      961 gatacgtatc agatacgtat cagggattgt tttaattcaa gcataaaaca acgggacaac
     1021 agtaaaagca gataaaacag tataaaaaat tttttaactg gatgtaaaaa tcattgacag
     1081 cagtatttta gttgtatcat gataaatcaa tagtttaatt aaatgttata aaatgtaata
     1141 caaaaatttg tatattaaaa ttatactaaa caagtgagag gaggattgga tggcacagtt
     1201 caagtcagaa tctatgtcag ctgtttttca ggagcaggct gctaagttgg gaagtaaagc
     1261 ctgtgtatca tataaaaaag gcagtcagtg ggtggatata tcctgggccg atatgaatac
     1321 catggtacac aatatggctt attatttaat ggattatggc attaagaaag gggacagggt
     1381 tgcaatcttt tcaccaaata gatgggaatg gtgggtggca agtttggcaa ccacttcaat
     1441 tggggcggtg agcgtaccaa tttatgccac taactcagct gaagaagcac agtatgtgct
     1501 ggaacattct ggctcttcta tttgctttgt tggtactgaa gaccatgctg acagggtttt
     1561 aaaagttatc aagaagtgcc ctaaactgga acagctagta atttttgatg atcttgcaaa
     1621 gaaaaagcag aatgttataa cctttaaaga agcactggaa aaaggtgcaa aaaaggcaaa
     1681 acctgctgaa tttgaaaaac gtttaaaagc tatcaaacgg gatgatgtgt ccacaatcat
     1741 ctacacttca gggactaccg gtaatcctaa aggggtaatg ctttcgcatt ataatttcta
     1801 ctccaatgct aaaaatatca tggctgatat gggcaagttt tttaatactg aggatgtttg
     1861 gctatcattt ttaccgttat cacactcgct tgagatgaca tgtggctttt atgggccaat
     1921 atgggttggc ggaaaaactg cgtttgctga aagcattgaa aagttgctgg ataactttaa
     1981 ggaagttcgt cccactgtca ttatcagtgt tccacgaatt tatgaaaaag ttcatgccgg
     2041 tattcttgca caggttgctg ctgcaccagg tttgaagaaa gcaattttca actttgcact
     2101 caacacggct aagaagaatc tgccgtatgt atgcagagac cttccacgta aaggattgtt
     2161 tgcaatacgg tacaacattg ctgacaagct ggtattttcc aagcttaaag ctgcaattgg
     2221 tatggataaa atgcgctatg ctatatctgg tggcgcacca ctttccgtgt ctgatgctga
     2281 attctttatt ggtatgggaa tgaagatact tgaaggcttt ggattaaccg aaacgacgcc
     2341 agtattaacc tataaccgac catggtttat taagccaggt acggtagggc aggctattcc
     2401 tgaaaccaag ataaagattg ctgatgatgg tgaaattctg gcaaaaggtc cacagattat
     2461 gttaggctat tataagaata aggctgctac tgaagaggtt atgactaaag atgggtattt
     2521 caaaactggt gatattggtg ttattgatga ggatggtttc ttgaagataa ctggccgaat
     2581 caaagatatt atcgtcacag ctggtgggaa gaatatatca cctcagaata ttgaaaatag
     2641 cgtaaagact tctccgtatg ttgagcagat agcggttatt ggcgataaac gtaagtattt
     2701 aagcgcactt gtaattccta actttgaagc attgggaaaa tgggccaagc agaaaggaat
     2761 cagcttcagt agcaatgcgg atctcattaa aaatgatgaa gtgaacaagc tcattgaaag
     2821 tgaaattgca aaatatacca agcagttttc acgcgttgag cagattaaga agttcacact
     2881 ccttgaggct gaatggacac aggcaacagg tgaattaaca ccaacacaga aggtaaaacg
     2941 aagaattatt gaacagaagt atgctaaaga aattgaggct atgtatccac ctgatattga
     3001 gtaaatcctt tatagtaaga taagcttgga agaggggtgg tcttagaccg cccttctttt
     3061 tttatggaaa aattttttaa aaaagcttga agttattgtt taacaatatc taaatataac
     3121 aggataatac ataacaattt atttgattta ttttataagg gggttctatg attaaaaacg
     3181 tcttaaaaat tgtaggtatt ctgattctaa tttttattat aattttattg gtcaaggcta
     3241 taactatttc tccaaagcag gttgaggtaa aagaagccat cccgtataaa attgacagta
     3301 tgattgttgc ccagcatctt tcagatgcta tacagattca aactgtatcc aatcaagatc
     3361 aggcaaaaac agatgttaaa cagtttgagc aatttcacaa atttttagca aaaacatatc
     3421 cggctttaca taaaaagcta aaaaaagaaa ttgttggggg atatgcactt ctatatacct
     3481 ggaagggctc tgacccctca ctcaaaccaa tgttacttat ggcacatcag gatgtggtac
     3541 cggttgagga gttaacgaaa gaccaatgga aatatccggg tttttcgggt acaatagcag
     3601 atggttttat atgggggagg ggagcgctgg atattaaaaa tcagttaatt tccataatgg
     3661 aggcagttga atttttaata aataaaggct ttgtcccgaa acggacaatc tatctggcat
     3721 ttggtcatga tgaagaggtt ggtggtgaag gtgccaaagc tatagcaaaa ctgctaaaac
     3781 aacgtaacgt tcagcttgaa tatgttcttg atgaaggtgg ggctattatg gagggcatgt
     3841 tgcctggcgt agaaacccct atagcattgg ttggaattgc tgaaaaagga tatttaaccg
     3901 taaaactaac tgtaaaaggt gaaggtgggc actcgtccat gccaccacat cacactgctt
     3961 tagggatttt atcaaaagca ctggtggatg tagaagataa tccgttccca aagaactttg
     4021 acagccccgc acggcagatg tttgaatatg ttgcacctca catgaagttt ccattacgtt
     4081 tgttgtttgc aaatatgtgg tgctttaaac cactgttaaa atggcaattt gaaaaatctg
     4141 aatcaaccaa tgctatgata cgcaccaccg ctgctgtaac catggccggc gcaagtgaaa
     4201 aagaaaatgt gctcccaact caagcctggg caatgatcaa ctgccgtatc cttcctggtg
     4261 aaaccatgga ttcaaccatt gcatatttga aaaaagtaat tggcaatgat gcagttacca
     4321 ttgaggcaac accgtggtct aatgaccctt cgccagtttc ggatatacag gcaggatcgt
     4381 ttacttcaat agcaactatc gcccggcaaa taaatcctga tgttattgtg gctccatacc
     4441 tggtgcttgg tgctactgat tcacgtcact acaatgcgtt aaccaatcag atataccgat
     4501 tttcaccagt taagatgaat tctgaagatt taaaacgtgt tcatggaata aatgaaagaa
     4561 tcagtatatc tgatatgggt aaaagcgtaa aatttttcat tgcattaatt gaaaaaactc
     4621 atatgtagca tgtgtggaag gtttgcacag attgaacctg tggatgtcat tgcacagcgt
     4681 ttgcaggttg aggaagtact ggtaca
//
LOCUS       DAOSZP010000108         4645 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_15283_length_4645_cov_3.783224, whole genome shotgun sequence.
ACCESSION   DAOSZP010000108 DAOSZP010000000
VERSION     DAOSZP010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4645
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15283_length_4645_cov_3.783224"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1354
                     /locus_tag="PK348_03450"
     CDS             <1..1354
                     /locus_tag="PK348_03450"
                     /inference="COORDINATES: protein motif:HMM:NF017828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HXK65302.1"
                     /translation="YEAGSRLLAGTKQEYDIALRYIPYFRDPVKEVFKAKAGQLFLQM
                     TATYLDNIVHAHQRGKKTCMTTFCFSPAIVYALDMVPVTLELLTVMMTFLYKRGTGEF
                     LDYCNEAGFTETSCSSQRGSMGALLADIGTPIDMVVTDSPGVCDTNANAFSFFAAYKD
                     LPFFQLNMPPWLVEERVTEYHVQDYKALVAFLEEQSGKKLDIDRLRQILTELQKQDEL
                     INELEQFLCYRPLPLPVTTCLFIYAGRFMFAGMPIYTRLLELLVNEGRENVRLHQQGI
                     YTSHEKSRGFFCYIDHYTHSLRLWQMLQELNIGYSGNILSHFWADSNPPVIQNNWEEA
                     AYSIKTGTLEDMLTSIAQINSRMPMIKSIRGPYDSPYMWLQDTLALASMYRADFIVYN
                     GTPGCRNTWGMVKLLAQDTEKAGFPTHIMYADAFDDRVQSWDATRDRFEEFLRVRRLI
                     "
     gene            1351..2136
                     /locus_tag="PK348_03455"
     CDS             1351..2136
                     /locus_tag="PK348_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012176185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HXK65303.1"
                     /translation="MIVAGCDIGSLTTKVVLFDGKKILASSIIKSKPKPEDGAYEALQ
                     QACAIATVNQDTITYCVGTGYGRKRVPFANDTISEISCHTKGAFYLYPNVRTIIDIGG
                     QDCKVISLNTQGEVDRFVTNDKCAAGTGRFLEVMAKVLKVELQELSNMAKKGNEAITL
                     ASACTVWAQADVIKYLNEGISKENIALGVCKSMAKRVGVLVNQASIKKDVCITGGVAK
                     NTAVVKELENLLGVTIIKITNVDPQLTGALGAALFAYQKAGGK"
     gene            2136..2924
                     /locus_tag="PK348_03460"
     CDS             2136..2924
                     /locus_tag="PK348_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015949611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HXK65304.1"
                     /translation="MIVAGCDIGSTTAKAVILDDSTIIATILLPVEGSPKDAALLLLS
                     SVQEKTGIQPSQIEAWCGTGYGQKNIPYASTVESEIVCHAKGVQFIDPDVRTIIDIGG
                     QDAKVCRIDAYGNVQRYVYNDKCASGTGRFLEITADVLEVPITELATLDAKGVKEVTL
                     SNQCVVFAETEVISLLNSGEDMGNIVKGLHRALASRVASLALSIGVEEKIAFTGGVAH
                     NAGMVGALERVLKTKLVVHEYCQMMGALGAALIAQERIKNRVAS"
     gene            2911..3582
                     /locus_tag="PK348_03465"
     CDS             2911..3582
                     /locus_tag="PK348_03465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65305.1"
                     /translation="MLHPDIKNVLEYVQNHKKGTIYSVKGKEPWPEVLHAAMIFKDDI
                     GCELGGPFGSLALHCMYDEVVDGRVIVIGSDMLQKQSVWGRLTLIDAAVDDEESYYSF
                     LQQVTLIPVRHSMEGVMIRIQPSEYKEWIRIHKDYIKTHTVIQLFSRLYTWYKQLPWV
                     RAVEFVAITSCSDDIALLLPLIEKHKSIIKAFEKRLSEDIKNCNACDNNAVCSTINSL
                     FEKGP"
     gene            3585..4328
                     /locus_tag="PK348_03470"
     CDS             3585..4328
                     /locus_tag="PK348_03470"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXK65306.1"
                     /translation="MDEAKTKVIAFCGKGGAGKTSLCALTIKYLAHSKKRVLAIDADP
                     AGGLSWALGLKPHKTLDDLRLSLHEAVGTKAVQSTDELYAMANYELLASLVEHAGFDF
                     LSVGRPEAEGCFCSINAFLRHCIGKLTEHYEYVCIDGEAGLEQVNRRVLETVTHLIIV
                     SDTSKRSIEIARNLFELARQRTKTMHCGIIINKMGDSSCQIDQLPVWGMLPVSRAIAV
                     YDSEGRSLLELSDNEEYYTMFKAILKKYM"
BASE COUNT         1423 a          820 c         1112 g         1290 t
ORIGIN      
        1 gtatgaagct ggttctcgtc tactggcagg aaccaagcag gaatatgata ttgcattacg
       61 ctatattcca tatttcaggg accctgttaa agaagttttc aaagcaaagg cggggcaatt
      121 atttttacag atgactgcaa catacctgga taatattgtt catgcgcatc agcgagggaa
      181 aaagacgtgc atgaccacat tctgcttttc accggcaata gtgtatgcac tggatatggt
      241 tcctgtaacg ctggaattgc tgacagtaat gatgacattt ttatataaac ggggtaccgg
      301 tgagttttta gattactgca atgaagctgg ctttacggaa acatcgtgtt cttcgcagcg
      361 tggatcaatg ggtgcactgt tagctgacat aggtacgcca atagacatgg ttgtcactga
      421 ttcaccgggt gtgtgcgata ccaacgccaa tgcattttct ttttttgcag catataaaga
      481 tttaccattc tttcaactca acatgccacc atggttggtg gaagagcgtg taaccgagta
      541 tcacgtgcag gattataagg cactggttgc gtttcttgaa gagcagagtg gcaaaaaact
      601 tgatattgat agattgcgcc agatacttac cgaactgcaa aagcaggatg aactcataaa
      661 cgagcttgag caattcctgt gttaccggcc tttaccattg cctgttacta cctgcctttt
      721 tatctatgca ggtcgcttta tgtttgctgg tatgcccatc tacacacgct tgcttgaatt
      781 gcttgtcaat gaaggaaggg aaaacgttag gctgcatcaa cagggaattt acacttcaca
      841 tgaaaaatca cggggatttt tttgctacat agaccattat acacatagct tgcggttgtg
      901 gcaaatgcta caggaactca atataggcta ttcaggaaat attttgagcc atttctgggc
      961 tgatagcaat ccgccggtaa tccagaacaa ctgggaagag gctgcgtatt ccattaaaac
     1021 cggaacactt gaagatatgc tgacaagtat tgcacagata aattcacgta tgccaatgat
     1081 aaaatccatt cgtggaccat atgattcgcc gtatatgtgg ttgcaggata cactggcgct
     1141 ggcaagcatg tatagggctg actttattgt ctataatgga acaccgggat gccgaaacac
     1201 ctggggtatg gtaaagttac tggcacagga taccgaaaag gcggggttcc ccacgcacat
     1261 catgtatgca gatgcatttg atgaccgcgt gcaatcgtgg gatgcaactc gtgaccgctt
     1321 tgaggagttt ttgcgtgtac ggaggctcat atgattgttg ctggctgtga cattggttcg
     1381 cttacaacaa aggtggtttt gtttgatggc aaaaagatac tggcttcatc aatcattaaa
     1441 tcaaaaccaa aaccagaaga cggcgcttat gaagcgctac agcaagcatg tgcgatagca
     1501 actgttaatc aggatactat tacatattgt gttggcactg gttatgggcg caaacgtgtt
     1561 ccctttgcaa acgatactat atcggagata tcgtgtcata caaaaggtgc attctatctg
     1621 tatcccaatg tgcgcaccat tattgatatt ggtgggcagg attgcaaagt tatttcactg
     1681 aatacacagg gcgaggttga tcgctttgtt accaatgaca aatgtgctgc aggaacaggc
     1741 cgctttttag aagtgatggc taaagtactg aaggttgaat tacaggaact atcgaacatg
     1801 gctaaaaaag gaaatgaggc tattacgttg gcatcagcat gtactgtgtg ggcacaggcc
     1861 gatgttataa aatatcttaa cgaagggata agtaaggaaa acattgcctt aggtgtatgc
     1921 aagtccatgg caaaacgagt gggagtactg gtaaaccagg caagcataaa gaaagatgtg
     1981 tgcattaccg gtggtgttgc aaaaaataca gcagtggtta aggaacttga aaatcttttg
     2041 ggtgttacca ttataaaaat aacaaatgtt gatccgcagc ttaccggagc attaggtgct
     2101 gcgctgtttg cttatcaaaa agccggaggt aaatgatgat agtagccgga tgtgatattg
     2161 gctctaccac agcaaaggct gttatacttg atgatagcac tataattgca acgatactgc
     2221 ttccagtgga aggaagcccc aaagatgcag cccttctgtt actttcttca gtacaggaaa
     2281 agacaggcat tcagccttca caaattgaag cctggtgcgg taccggctac gggcaaaaaa
     2341 atattcctta tgccagcacc gttgaatctg agattgtgtg ccatgcaaag ggcgttcaat
     2401 ttattgatcc agatgtgcgt accataattg atattggtgg gcaggatgct aaagtttgcc
     2461 gcattgatgc atatgggaat gtgcagcggt atgtatataa tgataaatgt gcttcgggta
     2521 ctggaaggtt tttagaaatc actgctgacg ttctggaagt acccattact gaacttgcca
     2581 ctcttgatgc aaaaggagta aaagaggtta cattgtccaa tcaatgtgtg gtgtttgctg
     2641 aaacagaagt tatttccctg ttaaacagtg gtgaagatat gggtaatatt gttaaaggat
     2701 tgcacagggc actggcatcg cgagtggcat cgcttgcatt aagtattgga gttgaggaaa
     2761 aaatagcttt tactggtggt gttgcgcaca atgctggtat ggttggtgca cttgaacgag
     2821 tccttaaaac aaaattggta gtacatgaat attgccagat gatgggagcg ttgggtgctg
     2881 cattaattgc gcaggaaagg attaaaaacc gtgttgcatc ctgatataaa aaatgtcctg
     2941 gagtatgtgc aaaaccataa aaaaggaaca atatattctg taaaaggcaa agaaccgtgg
     3001 cccgaggttt tacatgctgc aatgatattt aaagacgaca ttggatgcga gttgggtggt
     3061 ccatttggtt cattagccct gcattgtatg tatgatgaag tagttgatgg cagggtaata
     3121 gtaattggca gtgatatgct tcaaaaacaa tcagtgtggg gcaggttaac actgatagat
     3181 gcagcagtag atgatgagga aagttattat agctttttac agcaggtaac actgatacca
     3241 gtacggcact caatggaagg cgttatgata cgaatacagc cttctgagta taaagaatgg
     3301 ataaggattc acaaagatta tataaaaacg cataccgtaa ttcaattatt ttccaggtta
     3361 tatacatggt ataaacagtt gccgtgggta agggccgttg aatttgttgc aataacatca
     3421 tgcagtgatg acattgcctt gctgcttccg ctgattgaaa aacacaaaag tattataaag
     3481 gcatttgaaa agcgcctgag tgaagatata aaaaactgca atgcctgtga taacaacgcg
     3541 gtgtgttcta caattaactc gttatttgaa aaagggccat aaccatggat gaagcaaaaa
     3601 caaaagtcat tgccttttgt ggaaagggtg gagcaggcaa aacatcgctg tgtgcactta
     3661 caatcaaata ccttgcccat agcaaaaaac gagtgcttgc cattgatgct gatccagcgg
     3721 gaggcctttc gtgggcgctt ggattaaaac ctcacaaaac tcttgatgat cttaggttgt
     3781 cgttacatga ggcagttggc actaaggcag tgcagagtac tgatgaactg tatgcaatgg
     3841 caaactatga actacttgca agccttgtgg agcatgcggg ttttgatttt ttgagcgttg
     3901 ggcggcctga ggctgaagga tgtttttgca gtatcaatgc tttccttcgt cactgcattg
     3961 gcaaacttac agagcattat gagtatgtgt gtattgatgg tgaggcaggg cttgagcagg
     4021 ttaaccgccg cgtacttgaa acagtgacac atttaatcat tgttagcgat acatcaaaac
     4081 gaagcataga aattgcacgc aatctttttg aactggccag gcagcgtaca aaaactatgc
     4141 actgcggtat tattataaat aaaatgggcg atagttcctg ccagatagat cagcttccgg
     4201 tatggggcat gttgcctgta tctcgtgcca ttgcagtata tgacagtgaa ggaagaagtc
     4261 ttttggagtt aagtgacaat gaagagtatt acaccatgtt taaagcgata ctaaaaaaat
     4321 atatgtgaag ccagctaatc cttatttttc cttacacaaa cttcgcaccc ttaattgaaa
     4381 gtagatacta tggccattaa atcagggaaa aaatttttga tcatcagtgt gtatatttga
     4441 tgaaaggttg tgttccaaat agggaacgca gaatccataa caaaatgaaa aaaaagatat
     4501 cattacaata tcataatttt tacaaatatt aaaaactcat ggcaagataa aataatgatg
     4561 catgatgcag agaacatgga atacaagatt tttgtatttt tcttgaaata agaaatatca
     4621 cgaagtatac atataaagaa gtgca
//
LOCUS       DAOSZP010000109         4597 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_15449_length_4597_cov_3.199692, whole genome shotgun sequence.
ACCESSION   DAOSZP010000109 DAOSZP010000000
VERSION     DAOSZP010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4597
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15449_length_4597_cov_3.199692"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..873
                     /gene="ftsZ"
                     /locus_tag="PK348_03475"
     CDS             <1..873
                     /gene="ftsZ"
                     /locus_tag="PK348_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008516016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HXK65307.1"
                     /translation="ITAGMGGGTGTGAAPIVAEVAKECGALVVGVVTKPFRVEGRRRM
                     QHAEEGIKNLKEKVDTIIVIPNDLLLKIIDRNTPIDQAFKLADDILRQGVQGIADIIM
                     VTGLVNVDFADVRTVMKETGDAIMGVGIGVGENRAIEATQMAINSPLLEESGIDGAKA
                     VLLNIAGGNDLSLHEVNEVTEIINKQVDPDANIIFGATIDPALDDKIRVTVIATGFDR
                     KLNMLNRPNELDRATGTPLTVIEGRNNKERMEKKVPVQMKSFSLDYEKRRVKDYSHED
                     LDIPAFLRRSAESQ"
     gene            complement(874..1809)
                     /locus_tag="PK348_03480"
     CDS             complement(874..1809)
                     /locus_tag="PK348_03480"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXK65308.1"
                     /translation="MNTILLVDHTDYKHEIHKYLSQNYTVLMADSAYDAITTLNMQDV
                     DLVISQVELPGDNAFDLYNYLQKHYTYIPAIMITEKNMDTFFDKIFIQGIGNVLKIPV
                     NQNEFTNLVTKLITRKNIFGLENYVANIINTNAIRINASLQIQPAVEKILATIESWGF
                     TIKNKSVLNLILNEMIINAVYHSHGYTREKEMRIPIQLKEGQFVDITFCHNARTYAIA
                     ITDYQGTLTKEKILHSIHKAIEQEQLILKAAETGEDISDKISETGRGIDLMRKLASEF
                     YFIIQQKKRTEIILLFESDPNEKKSSSLKIIEDYQ"
     gene            1980..2450
                     /locus_tag="PK348_03485"
     CDS             1980..2450
                     /locus_tag="PK348_03485"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HXK65309.1"
                     /translation="MKKIMLLTLCIIFSFVIISDGGMAQDKEKKSAKEYIADLSSSDE
                     VTVVAAEEWIADNKEKSALPKLHELLRSDNRVKVRLYAAIALGEIANEESAESLNNAL
                     LNDPSADVRYSALLGIARIGSKSSYDAIQKARESEQDPFIKDLIKKMEDKFKGK"
     gene            2532..3317
                     /locus_tag="PK348_03490"
     CDS             2532..3317
                     /locus_tag="PK348_03490"
                     /inference="COORDINATES: protein motif:HMM:NF021613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HXK65310.1"
                     /translation="MESKVKYVVFDIESVPDANLIKQVKYPHLSISEHDAVIKFQEEI
                     LNVSDGSTWFIPVTFQLPVIVTMLEVDEKFFPVKMRTLDEPHFRTEQIVRQFWDVIEG
                     EYNDASFVTFNGRGFDFPLMELMAFRFGVTAKRHFKDKFASRFRFGTKHIDLHDWLSN
                     YSAIKMQGGLNLLAKVIGKPGKVEMSGDDVYDLYKAGKIEEIHEYCISDVLDTYFVFL
                     RTRVMVGELSYERENAIVQQTRVFLEQNKSNFKGFEQYLKNWK"
     gene            complement(3321..>4597)
                     /locus_tag="PK348_03495"
     CDS             complement(3321..>4597)
                     /locus_tag="PK348_03495"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HXK65311.1"
                     /translation="SKQNIFIGLFSLIVALRILVINEMLLISFFNPPWSINFRLSYLT
                     VTITPILFCHFVNSVYPDKIKKYTILTTDLYFGFFTLFVCTAPLHILSSSLVFIQLAL
                     VYISIRLLIILIKETSKNLKTKRIFYAGLILGFACLINDILFSHQIIKTINSSHFGIL
                     IFVVTQSMIAFEEYIIQQQRLVALSHEMNIAKKIQRNILPSEPPRLSGISVDIVYLPV
                     TTIGGDFYHFFEIDNKHTGIFIADVTGHGIPASMIASTVNIAFTLQYNHAAHPDIVFS
                     NINDLLIGKTGNQPITALYCYLDIDDMIVRLSRAGHPYPLYFNSKMGTVEEIKTQGNV
                     MGIWPSSDFHTAQFTIYNGDKIILYTDGLIEIRNKHNHQYNLDKLKKSIIENKHLKGS
                     EFTNTIINEVLQWAGSKENITDDITVITIDVG"
BASE COUNT         1514 a          693 c          928 g         1462 t
ORIGIN      
        1 atcactgctg gaatgggtgg tggtaccgga acaggcgctg cgccaattgt tgccgaagtg
       61 gcaaaagaat gtggtgcgct ggttgtaggt gttgtcacaa agccattccg tgttgaaggc
      121 cgaagaagaa tgcagcatgc agaggaaggg attaaaaatc ttaaagaaaa agttgataca
      181 ataattgtga tccccaatga ccttctgtta aagataattg atcgtaatac acctatagat
      241 caggcattta aactggcaga tgatattttg cggcagggtg tgcagggaat tgctgatatc
      301 atcatggtta ccggccttgt caatgttgat tttgctgatg tcagaaccgt gatgaaggaa
      361 accggtgatg caataatggg tgttggtatt ggtgttggtg aaaacagagc aatagaagca
      421 acgcagatgg caataaacag cccactgctt gaagagtcag gtattgatgg ggcaaaggca
      481 gtgctgctca acattgctgg tggcaatgat ttatcattac atgaagttaa tgaagtaaca
      541 gaaattatta ataagcaggt tgacccggat gcaaatatca tttttggggc aactatcgac
      601 ccggcacttg atgacaaaat aagggtaaca gtgattgcaa cagggtttga ccgtaaactt
      661 aatatgctga accgtcccaa tgaattggat agagccactg gcactccatt aacagttatt
      721 gaaggcagaa ataataagga aagaatggag aaaaaagttc cagttcagat gaagtctttt
      781 tcactggatt atgaaaaacg gcgggtaaaa gactattcac atgaggatct ggatatccca
      841 gcgtttttgc gccgaagtgc cgaatcccag tgattactgg tagtcctcaa taatttttaa
      901 tgagcttgat ttcttttcat tggggtctga ttcaaaaagc aggataattt ctgttcgttt
      961 tttttgttga atgataaaat aaaattcaga agctaattta cgcataaggt caatgccgcg
     1021 tccagtttct gaaattttat cgctgatatc ttcaccggtt tcagctgctt taagaatcag
     1081 ttgctcctgt tcaatggctt tatgaatact gtgtaatatt ttttctttag taagagttcc
     1141 ctgataatca gttattgcaa tagcatatgt cctggcatta tgacagaagg tgatatcaac
     1201 aaattgacct tctttaagct ggataggaat tcgcatttcc ttttcacggg tatacccatg
     1261 tgaatggtac actgcattga taatcatttc attaagtatt aaatttaaaa cgcttttatt
     1321 tttaatagta aatccccatg attctatggt agccagtatt ttttctactg ctggctggat
     1381 ttgaagagaa gcattaatgc gtatggcatt tgtattaatt atgttggcta cataattttc
     1441 caggccaaaa atattttttc gtgtgataag tttagtaaca agattggtaa attcattttg
     1501 attaactggt atcttcaata cattaccaat accctgaata aagattttat caaaaaaggt
     1561 atccatattt ttttcagtaa tcataattgc tggtatatag gtgtagtgct tttgtaaata
     1621 attataaaga tcaaaagcat tatcgccagg caactcaacc tgtgaaatta ccagatcaac
     1681 atcctgcata tttaatgttg taattgcgtc atatgctgaa tctgccatca aaacggtata
     1741 attttgtgaa agatatttat gtatttcgtg tttatagtct gtatggtcaa ctaataatat
     1801 cgtattcata gaagctcatt actttaaaaa atttacctgg cgatagttcc tgtcaatagt
     1861 aactatcgca aaaattatat atttatataa gaaaatcatt gacattcttt tcaaaaaaag
     1921 gatactatac ataaaatata ctatcgtcaa tactatcgtc aaaaaatgag aggtgcacaa
     1981 tgaaaaaaat aatgttacta acattatgta ttattttttc gtttgttata atttctgatg
     2041 gaggaatggc ccaggataag gaaaagaaaa gtgctaagga atatatagcc gatctttcaa
     2101 gcagtgatga agtaactgtt gtggctgcag aggagtggat tgcagataat aaggaaaagt
     2161 cagcacttcc aaaattacac gaacttctgc gcagtgacaa cagagtgaaa gtgcgtttat
     2221 atgcagctat agcgttgggc gaaattgcca atgaagaaag tgcagaaagc ctcaataatg
     2281 cattgcttaa tgaccccagt gctgatgtcc gctattcagc attattaggg atagcacgta
     2341 taggttcaaa aagttcatat gatgctattc agaaagcacg tgaaagtgag caggatccat
     2401 tcattaagga cctgatcaaa aaaatggaag ataagtttaa aggaaaataa attattaata
     2461 ttagatttta tgtaaaaagc atgtggggta atctccacat gcatttttat tttattatta
     2521 gggttgtaaa cgtggaatcc aaagttaaat atgttgtttt tgatattgag tcagtgcctg
     2581 atgcaaactt gataaaacag gtgaagtatc ctcatttatc tattagcgaa catgatgctg
     2641 ttattaaatt tcaggaagaa atattaaatg tatctgatgg atccacgtgg ttcattcccg
     2701 ttacattcca gttgcctgta atagtaacca tgcttgaagt tgatgaaaag tttttcccag
     2761 taaagatgcg gactctggat gagcctcatt tcaggaccga acagattgta aggcagttct
     2821 gggatgttat tgaaggtgaa tataacgatg cctcttttgt tacgttcaat ggtcggggat
     2881 ttgatttccc cttaatggaa ctgatggctt ttagatttgg ggtaactgcc aaacgacatt
     2941 tcaaagataa atttgcatcc cgtttcaggt ttggcaccaa gcatattgat ctgcacgact
     3001 ggctatccaa ttacagtgca ataaagatgc agggtgggtt aaacttactt gcaaaggtaa
     3061 taggtaaacc tggcaaggtt gaaatgagtg gtgatgatgt gtatgatctt tacaaagcag
     3121 gaaagattga agaaattcat gaatactgta tctcagatgt tcttgacaca tactttgtgt
     3181 ttttacgaac aagagtaatg gtgggtgaac taagctatga aagagaaaat gcaattgttc
     3241 agcaaaccag agtttttctg gaacaaaata aatcaaattt taaaggattt gagcagtatt
     3301 taaaaaactg gaaatagatt tcaacctaca tctattgtaa ttacggttat atcatctgta
     3361 atgttttctt tgctaccagc ccattgtaat acttcattaa taatcgtgtt tgtaaattca
     3421 ctgcctttca gatgtttatt ttcaatgatg gattttttaa gcttatccag attgtattgg
     3481 tgattgtgtt tatttcgtat ttctattaat ccatcagtat aaagaattat tttatcacca
     3541 ttgtatatag taaattgtgc tgtatgaaaa tcagatgatg gccatatacc catgacgttt
     3601 ccctgtgttt taatctcttc aacagtaccc attttagaat taaaatataa tggataggga
     3661 tggcctgccc gggataaacg tactatcata tcgtctatat caagataaca gtataatgcc
     3721 gtaataggct gattaccagt tttgccaatc aaaagatcat tgatattact gaatacaata
     3781 tcaggatgtg cagcatgatt atactgtaat gtaaaagcaa tgttaacagt tgatgctatc
     3841 atggatgctg gaattccatg tccagtaaca tctgcaataa atattcctgt gtgtttattg
     3901 tctatctcaa aaaaatgata aaaatcacca ccaatggtgg taaccggtaa atatacaatg
     3961 tcaactgaga tgccagaaag acgaggtggt tcagatggca aaatatttct ttgtattttt
     4021 tttgcaatat tcatctcatg tgacagtgca accagccttt gttgttgaat gatatattct
     4081 tcaaaagcta tcattgattg agttacaaca aatataagaa taccaaaatg gctggagttt
     4141 attgtcttta ttatttgatg cgaaaacaat atatcattta taagacaagc aaaacctaga
     4201 ataaggccag cataaaaaat tcgctttgtt tttaagtttt tgcttgtttc tttaataaga
     4261 ataatgagaa gacggatgga aatatataca agtgccagtt gtatgaatac cagggaagaa
     4321 gataagatat gcagaggagc agtacaaaca aataaagtga aaaatccaaa gtaaaggtct
     4381 gttgtaagta tggtatactt tttaatttta tctggataga cgctatttac gaaatggcaa
     4441 aaaagtattg gtgttatggt tacagtcagg tatgatagcc tgaaattgat gctccatggt
     4501 gggttgaaaa agcttattag aagcatttcg ttgataacaa gaatgcgtaa agccacaatc
     4561 aatgaaaaaa gccctatgaa aatattttgc tttgacc
//
LOCUS       DAOSZP010000110         4593 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_15464_length_4593_cov_3.580873, whole genome shotgun sequence.
ACCESSION   DAOSZP010000110 DAOSZP010000000
VERSION     DAOSZP010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4593
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15464_length_4593_cov_3.580873"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..428
                     /locus_tag="PK348_03500"
     CDS             <1..428
                     /locus_tag="PK348_03500"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HXK65312.1"
                     /translation="TPEGLYHITEILSMDAPKKTSAYKTLLEMNKVYFKAKDGHYKFG
                     HPDVDLGDNAYGPRFYRISYPNTDDLARYEEAKKNGMLPQNKGKVPSIGSGIAIHGNN
                     DPDSIGQLASSGCIRMYNDDIIELEQYIQVGTPVLILPD"
     gene            588..896
                     /gene="yajC"
                     /locus_tag="PK348_03505"
     CDS             588..896
                     /gene="yajC"
                     /locus_tag="PK348_03505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HXK65313.1"
                     /translation="MFPFEIAYAANGQQASSPWVSIVPLVLMILIFYLLLIRPTQKKE
                     KERMKMIDQLKKGDKVLTSSGMIGVVVNIKPEENIITLKLAEGVKVDFVKSAIQQKLS
                     "
     gene            935..1531
                     /locus_tag="PK348_03510"
     CDS             935..1531
                     /locus_tag="PK348_03510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65314.1"
                     /translation="MKQCRNIFLVLCVACMVVLCSTVMHVYAQETPSTTVQSPENTPG
                     NETPVQELSPQIQKEIPKTEGNEPQVVKKAESADATKKEEKKPAVTATEATQKTDAPK
                     GLKVDSKSLLSITDGAFRYSRIPGITIKENNEQILAHIEQEKQNIDTEEKGLFGLSKQ
                     ASDRMAKIILVVIIIFVFILYRMRTRSTSGSVLRRFPK"
     gene            1556..3211
                     /gene="secD"
                     /locus_tag="PK348_03515"
     CDS             1556..3211
                     /gene="secD"
                     /locus_tag="PK348_03515"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HXK65315.1"
                     /translation="MTRGLLYKAIFIICIVCFAVMLILPTIGENTLQIVLSQTSTEQD
                     LQTIKQRFPESKYKIVINNKEVLVEGYNLNVAVMNEIKRYPGVVDAKLLPHWAEKALL
                     AKKINLGLDLQGGSYLVLQANYHRIEEKTGKPLTNAEKNEITEQALELLRNRVDKFGV
                     AEPSIRPKGNDAIEIQLPGVKDPESVKKAIGTTGRVEYRLVDDTYTQKANQWLKEHHA
                     GKPLPEDQLELNKLTDTIKTAIALPDSLELLYYYERNPQTAKLFPAYPIVLEKKVALA
                     GNDISKAWVAPDEYQQLAVHFNTTPEGAAKFAEVTGEKNHGKKLAIVIDDKVRSAPSI
                     RVQINTGQAIIQGDFTYEEVTALARIIKEGALPVDLKIVEERTVGPTLGIDSIQNGIK
                     AFIVGVGGIMLFMLIYYKFAGLIADIGLILNGIFMLALLSWLGFTLTLPGIAGFILTM
                     GMAVDANVIIYERIKEELRTGKTPRAAVAQGFGRAFWTIFDSNITTLFSAFVLSQFGT
                     GPIKGFAVTLSIGLIVSMFVALYITKFIFEIMTLRGSLKKLSI"
     gene            3223..4113
                     /gene="secF"
                     /locus_tag="PK348_03520"
     CDS             3223..4113
                     /gene="secF"
                     /locus_tag="PK348_03520"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HXK65316.1"
                     /translation="MEKIIPFIRYRFIAFAITITLIMIFSLQTYRNGGFNWGIDFVGG
                     IKLTVKFEQGVDTAQIRKVLTQNKISAVIQQVGKEEDNEYLISIKLASRAKNVEESTK
                     ELKAVIQSNFKNVEILSIETVGPAIGDFLKRSAVKLIALSVIIMTLYLAFRFELKYSI
                     GAMVALVHDVILSVLFCGFAGIEINIPIVAAILTIYGYSVNDTIIIFDRVRENLQIKT
                     KQTFIDVIDKSITQTISRTLLTALTTLFAVFALYMISGEGLNEFASVLLFGIVIGTYS
                     SMYIASPFVLYWEKLTLKKA"
     gene            4118..>4593
                     /locus_tag="PK348_03525"
     CDS             4118..>4593
                     /locus_tag="PK348_03525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65317.1"
                     /translation="MPLRHIIILIVCIVASCNVFAQGLDNPYSYTAIAGFANQLVEQR
                     EYYRAYQEYQRLYYYFPSLYSYDAFLIASLYCHYQGKQYHSVLEKVKNQSPINLPVWI
                     YSFDSAFSGMLLPPQMMDVQWQKDVNPEYEAMILKRKLAYSLLTVDNYNELSPYTVL"
BASE COUNT         1477 a          794 c          996 g         1326 t
ORIGIN      
        1 gtacgccaga ggggctatat catataactg aaattttaag catggatgct ccaaaaaaaa
       61 caagtgccta taaaactctg ctggaaatga ataaagtata ttttaaggca aaggatgggc
      121 actataagtt tggtcatcct gatgttgatt taggcgataa cgcctatgga ccacgatttt
      181 atcgtatttc atatccaaat actgatgacc tggctcggta tgaagaagca aaaaagaatg
      241 gcatgttgcc acaaaataaa gggaaggtgc catcaatagg tagcggtatt gcaattcatg
      301 gcaataatga tccggattcc attgggcagc tggcatcaag cggttgcata cgtatgtata
      361 atgacgatat aatagaatta gagcagtata ttcaggttgg tacaccggta ttaatacttc
      421 ctgattaaaa atattgcata aattgtgttg acatttgcaa ttcattgcat aatggttgca
      481 gtgattttat ctgtgtattt attgagtaaa agcatatcag gctttctatg ggtctgaaca
      541 tgctttatgt gaatctacta actaaaacat aaaagtgagg taacaacgtg tttccatttg
      601 agatagcata tgcagccaat ggacagcagg catcaagccc atgggtaagc atagtcccgc
      661 ttgtcttgat gatattgatt ttttatcttc ttttgatacg gccaacacaa aagaaggaaa
      721 aagaacgcat gaagatgata gaccagctta aaaaaggcga taaagtcctg acatcaagcg
      781 gaatgattgg cgtggtggta aatattaaac ctgaagaaaa tatcattacc ctgaaacttg
      841 cagaaggcgt aaaggtggac tttgtcaaaa gtgctattca gcaaaagtta tcgtgatgta
      901 ttaatgataa aatggtattg acaggaacaa tagtatgaaa cagtgccgaa atatttttct
      961 ggtattgtgt gttgcgtgta tggtggtgct gtgctctaca gtgatgcatg tgtacgcaca
     1021 ggaaacaccg tccactactg ttcagtcacc agagaataca ccgggcaacg aaactcctgt
     1081 acaggaacta tcgccccaga ttcaaaaaga aatacctaaa actgaaggta atgaacctca
     1141 ggttgtgaaa aaagctgaat cagcagatgc cacaaaaaaa gaagaaaaga agcctgcagt
     1201 tacagcaact gaagcaacgc aaaaaactga tgccccaaaa ggattgaaag tggatagtaa
     1261 gtcgctgctc agtattactg acggcgcttt cagatattcc cgcattcccg gtattaccat
     1321 taaagaaaat aatgaacaga tacttgcaca catagaacaa gaaaaacaga atattgatac
     1381 tgaggaaaag ggtttatttg gtcttagcaa acaggcatct gaccgtatgg caaaaattat
     1441 tttagtagtg atcattattt ttgtttttat cctctatcgg atgaggacca gaagtacttc
     1501 aggtagcgtt ttgcgcagat ttccaaaata atacataata atgcatgagg tgtacatgac
     1561 acggggatta ttgtataaag ctatttttat aatctgtatt gtgtgttttg ctgttatgct
     1621 cattttgcct accattgggg aaaataccct gcagatagtg ttaagccaga catctaccga
     1681 acaggattta cagacgataa agcagcgatt tcctgaatca aagtataaaa ttgtgattaa
     1741 taataaagaa gtactggttg aagggtacaa tcttaatgta gcagtaatga atgaaataaa
     1801 acgctaccct ggcgttgtgg atgccaaact tttaccacac tgggctgaaa aggcgctgct
     1861 tgcaaaaaaa ataaatttag gtcttgattt gcagggtggc tcatacctgg tgttacaggc
     1921 aaactatcac aggatagaag aaaaaacggg caagccactg acaaatgctg aaaaaaacga
     1981 aattaccgaa caggcactgg aattgcttcg taaccgtgtt gacaagtttg gtgtagccga
     2041 gccttcaata cggcccaagg gcaacgatgc cattgagatt cagttacccg gggttaaaga
     2101 tccggaaagt gtaaaaaaag caattggcac caccgggcgt gttgagtatc ggctggttga
     2161 tgacacctat acccagaaag ccaatcagtg gctaaaagag catcatgcag gcaaaccgct
     2221 gcctgaggat cagttggaac tcaataagct taccgacaca attaaaactg caatagcgct
     2281 tcctgattcc ttagagttgt tatattatta tgaacgcaat ccgcaaacag ccaaattatt
     2341 tccggcatac ccaatagtcc ttgaaaagaa ggttgcactg gcaggcaatg acataagcaa
     2401 ggcatgggtt gctcctgatg aataccagca gttagcagtt cattttaata ccacaccgga
     2461 aggtgctgca aaatttgcag aagttaccgg tgaaaaaaat cacggcaaaa aacttgccat
     2521 agttattgat gataaagtcc gcagtgcacc atcaatcagg gtgcagataa ataccgggca
     2581 ggcaatcatt cagggtgatt ttacctatga ggaagtaacc gcacttgcac gaattattaa
     2641 agaaggtgca ttacctgttg atttaaagat agtagaagag cgaacggtag gtccaacact
     2701 tggtatcgat tcaattcaaa atggtataaa ggcctttatt gttggcgttg gtggtataat
     2761 gctatttatg cttatttatt acaaatttgc aggactgata gccgatatag gcttgattct
     2821 taatggcata ttcatgttag cgttactatc gtggttggga tttacactta cgcttcccgg
     2881 tattgctggt tttatcttaa caatgggtat ggcagtggat gccaatgtta ttatttatga
     2941 acgaataaaa gaggaactgc gaactggcaa aacaccacga gcagcagtgg cgcagggttt
     3001 tggaagagcc ttctggacaa tctttgactc aaatatcacc actttgtttt cggcatttgt
     3061 attgtcgcaa tttggaaccg gtccaattaa agggtttgcg gtaacccttt caataggtct
     3121 tatcgtttct atgtttgttg cgctgtatat tacaaagttt atttttgaaa taatgacgtt
     3181 acgtggatca ctgaaaaaat taagtattta aggagtggta ccttggaaaa gataattcct
     3241 tttataagat acagatttat tgcttttgca attacgataa cattgattat gatattttca
     3301 ttgcagacat atcgcaatgg tggttttaac tggggtattg attttgttgg tggtattaag
     3361 cttactgtaa agtttgagca gggggttgat accgcacaga tacgtaaggt tttgacccag
     3421 aataaaatca gtgcagtgat tcagcaggtg gggaaagaag aggataatga atatctgatt
     3481 tccatcaaac ttgccagccg tgccaaaaat gttgaagaaa gcaccaaaga actgaaagct
     3541 gttattcaga gtaattttaa aaatgttgaa atattgagta ttgaaactgt gggtcctgca
     3601 attggtgatt ttctcaaaag atcggcggtg aaactgattg cactttcagt tataatcatg
     3661 acgttatatt tagcattccg gtttgaattg aagtattcca ttggtgcaat ggttgcactg
     3721 gtacatgatg ttatactatc agttcttttc tgcggatttg caggtattga gattaatata
     3781 ccgatagtag ccgcaattct taccatttat ggatactcgg taaacgatac cattatcata
     3841 tttgaccgtg tgagagaaaa tttacagatt aaaaccaagc aaacatttat tgatgtgata
     3901 gataaatcta tcacacagac aatatccaga acgctgctta ctgcgcttac cacattgttt
     3961 gccgtatttg ccctgtatat gattagtgga gaaggcctta acgaatttgc cagtgtactg
     4021 ttgtttggta tagttatagg aacgtattca tctatgtata tagcatctcc gtttgtgtta
     4081 tactgggaaa aactgacatt aaaaaaagcg taagcgtatg ccgttgcggc atattattat
     4141 tctgatagtg tgtattgtag caagctgtaa tgtctttgcc caaggattag ataatcccta
     4201 tagttatact gctatagcag ggtttgcaaa tcaactcgtt gaacaaaggg aatactaccg
     4261 cgcatatcag gaataccagc gcttatacta ctacttccct tcgttgtatt cgtatgatgc
     4321 ttttcttatt gcttcattgt attgccatta tcagggcaaa caataccatt cagttcttga
     4381 aaaagtgaaa aatcaatccc caataaacct gccggtgtgg atttatagct ttgacagtgc
     4441 attcagcggt atgctgttgc caccacagat gatggacgtg caatggcaaa aagacgtcaa
     4501 tccggaatat gaagcaatga tactgaaaag aaagcttgca tattcattgt tgacagttga
     4561 taattacaat gaactatcgc cctatactgt tct
//
LOCUS       DAOSZP010000111         4583 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_15504_length_4583_cov_2.659231, whole genome shotgun sequence.
ACCESSION   DAOSZP010000111 DAOSZP010000000
VERSION     DAOSZP010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4583
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15504_length_4583_cov_2.659231"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(126..1331)
                     /locus_tag="PK348_03530"
     CDS             complement(126..1331)
                     /locus_tag="PK348_03530"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HXK65318.1"
                     /translation="MANTTSTLVSKRVINRLFEPACARFTVGMLISSVYYVYLDTTIE
                     KIVDELSNNDKIFALAVVDKSMQVSGIIIRKDLFDILGRRFGRELYYNKTAADMLQKV
                     QLFDYTMHIFECSKLIEKDLLTGVQDYYALVDENGCYKGLFSTKDMLIFLSRLTQIDI
                     EQAKRVINRMIKKQYELKTKRLTVSIRNELVQDIGGDSYFIIPSKKENQLIVAICDVA
                     GKGVAASMITTLINGMVTTYDFNSSDIDTFLVHLNNYIFSTFEGEKFVTACFMVFNED
                     NGILTLYDFGHSMTYLYRGNNVYKLALKEIKDCNMPLGIQKITAMHSGKINLESGDTI
                     ITITDGCVEQRDVNCKEYGIQRFNKVFVSSYSDDLAKTLHAIMTDIQNFRTPYPQLDD
                     ITIACVRYE"
     gene            1528..2007
                     /locus_tag="PK348_03535"
     CDS             1528..2007
                     /locus_tag="PK348_03535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65319.1"
                     /translation="MNRLLIVIFILLFTLHHSFANESLNCITSHTSALGFKVDLPDWD
                     KSEMMRGISHEFICAYSKTVYIKIYKVKDSQTDMLHELTWNIWEKDPNAHFIIKTSTE
                     DGGIIVTTYRSQGTSIHRIRLIHNNDTVFIIECSAPEKAFYKYEVYFNRVFQSFSVL"
     gene            complement(1996..2895)
                     /locus_tag="PK348_03540"
     CDS             complement(1996..2895)
                     /locus_tag="PK348_03540"
                     /inference="COORDINATES: protein motif:HMM:NF012267.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HXK65320.1"
                     /translation="MKAILKPSTIAIFKKHGWRVDRAIHNYLYFVFYYPYVFILYHTF
                     KFLTDHFSWFTPLRHFLKFAFDRYHAKILSFGDTRKILELNRDIAHTSDSNRSIIPYK
                     FAYKIIFTEPDFIAVMDCPCKKTMGDEEWAIHSCIAIGKPVASFWLEHGKKYNAERIT
                     QHQALELIKNFRKHGYITQAFFKVATGGSTGVICNCHPKSCVSLKATQFAKKFDDSLS
                     MNAAAGYSVVHDETKCKHCGDCSRICPFNAISVSNTSWMYNKNDCAGCELCVEHCTHN
                     ALSMDIDKSKPRPLDIAALPLKY"
     gene            complement(2916..>3407)
                     /locus_tag="PK348_03545"
     CDS             complement(2916..>3407)
                     /locus_tag="PK348_03545"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXK65321.1"
                     /translation="QGTIEVESEPCKGTTFYIYIPATKKEPAEPKKVEERKVSKGGRI
                     LVLDDEPMMREIMLKFLEHLGFNAVAVDDGRDAINMYIKEKESGTPFNALIFDMTIKG
                     GMGGREAIEEIRKYDKDILAFVMSGYHEDPVLAHPQDYGFNGGLCKPFTLEELEAMLA
                     RYF"
     assembly_gap    3408..3417
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3418..>4583)
                     /locus_tag="PK348_03550"
     CDS             complement(<3418..>4583)
                     /locus_tag="PK348_03550"
                     /inference="COORDINATES: protein motif:HMM:NF020037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HXK65322.1"
                     /translation="QGLDSYEFRIISKDGQVKWVYVTSATVQYKGKPAGLVSVLDITD
                     RKKAEEALFEEKEKLRITLQSIGDGVIATDTDGRVVIINETAQQLTGYSQVEATGKHL
                     SEIFTIVHELSAKPLENPVDKVLSTGHVYELSNHTVLVAKDGTRRVIADSAAPIKDKA
                     GNIVGVVLVFRDMTEKLQLIEQIQRAQKLESLGLLACGIAHDFNNILEGVFGYIGLAS
                     AYVSDSTVSEMLANALKSIQRAKGLTGQLLTFSKGGQPVKKVQSIIPLLQDTVQFAIS
                     GSNVKAQFSVDDNLDYAEIDYNQISQVIQNIVINAVQAMPMGGIITIGAGNISFAEGE
                     HPLLQGDYIRVTIADQGIGIPKEVLPKIFDPFFTTKSMGQGLGLATSYSIIARH"
BASE COUNT         1468 a          919 c          673 g         1513 t
ORIGIN      
        1 ttatcagctc agatattaaa caactatcac cacaattaaa aaccgagaat gttattaccc
       61 ttactatacg cggaatagaa aagccaattg aagtatatac aatagcttgc aatgaaaatt
      121 gattgtcatt cataccgtac acaagcaatt gttatatcat caagctgggg atagggggtt
      181 ctaaaatttt gtatatctgt cataatagca tgtaatgttt ttgcaagatc atctgaataa
      241 ctgcttacaa acaccttatt aaatctttga ataccatatt ctttacaatt aacatcacgt
      301 tgttccacac aaccatcggt aattgtgata atagtatcgc cagactctag attaatttta
      361 cctgaatgca tggcggtaat tttttgaata cctaatggca tattacaatc ttttatttct
      421 tttaatgcca atttatatac attatttcct cgataaaggt aagtcattga atgtccaaaa
      481 tcatataacg tcaatatgcc attatcctca ttaaagacca taaaacaagc agttacaaac
      541 ttttcccctt caaatgtaga aaatatataa ttgtttaaat gcactaaaaa ggtatctata
      601 tcactactat taaaatcata tgttgtcacc ataccattta tgagtgttgt aatcatcgaa
      661 gcagcaacac ctttaccagc aacatcacat atagcaacaa taagttgatt ttcctttttt
      721 gatggtatta taaagtatga atcaccacca atatcctgaa ccaattcatt tctaattgaa
      781 acagttaacc gctttgtttt aagttcatat tgtttcttta tcattctatt tataacacgt
      841 tttgcttgct ctatatctat ctgtgttagt cttgataaaa atatcaacat atctttagta
      901 gaaaacaatc ctttgtaaca tccattttca tcaacaaggg cataataatc ctgcactcca
      961 gttagtaaat ctttttcaat tagttttgaa cattcaaaaa tatgcatagt ataatcaaac
     1021 aattgaacct tttgcaacat atcagccgca gttttattat aatagagctc tcttccaaaa
     1081 cgtctcccta aaatatcaaa aagatctttc ctgataatta tacctgatac ctgcatggat
     1141 ttatctacta ctgccagggc aaatatttta tcattattcg atagttcatc aacaatcttt
     1201 tcaattgttg tatctaaata tacataataa actgaactta ttagcatacc aactgtaaat
     1261 ctggcacatg caggttcaaa taatctattt attactctct ttgaaaccaa tgttgatgta
     1321 gtgtttgcca tgatagtcaa ttcctctgat gattattttt gttttattgt gaaaatgtaa
     1381 ttataaaagg ttaaaaaatt attaaaagaa catgaagaaa tattaatttt agtattaaca
     1441 atctattacc aaaaaaattg ttgcaaattc atccataatt atctatttct aagacgctat
     1501 aaaaactctt aaaaaagagg acaatccatg aataggttat tgatcgttat atttatttta
     1561 ctatttacat tgcatcattc ttttgcaaat gaatcattga attgtatcac ttcccataca
     1621 agcgccttag gttttaaagt agaccttcct gactgggaca aatcagaaat gatgaggggt
     1681 atttcccatg aatttatttg tgcgtattca aaaacagtgt atatcaaaat atataaagtt
     1741 aaagacagcc aaactgacat gctccatgaa cttacgtgga atatttggga aaaagatcca
     1801 aacgctcact ttattatcaa gacatcaaca gaagatggtg gaattattgt tacaacatat
     1861 agaagtcaag gcacatccat tcacaggata aggctcatac ataataatga tacagttttt
     1921 attattgagt gcagtgcacc tgagaaagca ttctacaaat atgaagttta ttttaacagg
     1981 gttttccaga gtttttcagt actttaatgg caatgctgca atatctaaag gacgtggttt
     2041 tgatttatca atatccatcg ataatgcgtt atgtgtgcag tgttccacac agagttcaca
     2101 tccagcacaa tcatttttat tatacatcca tgaagtgtta cttacactga ttgcattgaa
     2161 agggcatatt cttgaacaat ccccgcaatg cttgcatttt gtttcatcgt gtactacact
     2221 ataccctgcc gcggcattca ttgataatga atcatcaaac tttttagcaa actgtgttgc
     2281 ctttaagctg acacaggatt ttggatgaca gttgcatata actcccgtac tgccaccagt
     2341 agcaacctta aaaaaggcct gtgtaatata tccatgcttc ctaaaatttt taataagttc
     2401 aagtgcctga tgttgggtta ttctttctgc attatatttt tttccatgtt caagccagaa
     2461 cgatgcaaca ggtttaccaa tagcaataca ggaatgtatt gcccattctt catcacccat
     2521 agttttttta caggggcaat ccatcactgc aataaaatca ggctcagtaa agatgatctt
     2581 atacgcaaat ttgtaaggga taatgcttct attggaatca ctagtatgtg caatatccct
     2641 gtttaattct aaaatttttc tggtatcacc aaatgataat atcttggcat gataccgatc
     2701 aaacgcaaat ttaagaaaat gtcgcagtgg tgtaaaccag gaaaaatgat cagtcaaaaa
     2761 tttaaatgta tgataaagta taaacacata aggataatag aagacaaaat acaaataatt
     2821 atgaatagcc ctgtcaacac gccacccatg ctttttgaat atagctattg ttgaaggttt
     2881 gagtattgct ttcaatgttc ctcttacata ctatatcaaa aataccttgc taacattgcc
     2941 tctaattctt ctaaggtaaa aggcttacac aatccaccgt taaagccata atcctgggga
     3001 tgtgccagca ccggatcttc atgataaccg ctcatgacaa atgccagaat atctttatca
     3061 tactttctaa tctcttcaat agcctctctg ccacccatcc cgcctttaat ggtcatatca
     3121 aagattaaag cattaaatgg tgttcctgat tctttttctt ttatatacat atttattgca
     3181 tccctgccat catccaccgc tacagcatta aaacccagat gctccagaaa ttttagcata
     3241 atttcgcgca tcataggttc atcatccagc accagaatcc tgccaccttt tgatactttc
     3301 ctttcttcaa ctttctttgg ttctgctggt tctttttttg ttgcgggaat ataaatataa
     3361 aacgtcgtac ctttgcaagg ctctgattca acttcaatgg taccctgnnn nnnnnnngat
     3421 gacgtgcaat aattgaatag cttgtagcaa gccctaatcc ctgtcccata cttttggtag
     3481 taaagaaagg atcaaaaatt tttggcaaaa cctctttagg aataccaata ccctgatcgg
     3541 cgatagttac cctgatataa tctccctgca ataatggatg ctccccttca gcaaaggata
     3601 tattgcctgc acctattgtg atgattcccc ccattggcat tgcctgcaca gcattgatga
     3661 caatattttg aattacctgt gaaatttgat tataatcaat ttcagcataa tcaagattat
     3721 catcaacaga aaactgtgct ttgacattag aaccgcttat tgcaaactgc actgtatcct
     3781 gaagaagcgg aataatagat tgaaccttct taacgggctg gccgcctttt gaaaatgtca
     3841 acaactgccc tgttaaccct tttgcacgtt gaattgactt cagcgcattt gccagcatct
     3901 cagaaacagt gctatcagat acatatgcac ttgccagccc aatgtatcca aatactcctt
     3961 caagtatgtt attgaaatca tgtgcaatcc cacatgccag aaggccaagg gattctaatt
     4021 tctgtgctct ctgtatttgt tcaatcaact gcagtttttc agtcatatcc ctgaatacca
     4081 gtaccacacc tacaatattg cccgctttat ccttaattgg tgcagcactg tcagcaatga
     4141 cacgtcgcgt gccatctttt gccacaagta cagtatgatt tgacagttca tatacatgcc
     4201 cagtagataa cactttgtca acggggttct ctaatggttt ggccgatagt tcatggacaa
     4261 tagtaaaaat ttccgaaagg tgcttgcctg tagcttctac ctgtgaatat ccagtaagct
     4321 gttgtgctgt ttcattgatg attaccaccc ttccatcagt atccgtggca ataacaccat
     4381 caccaatact ttgcaaagta atacgcaact tttctttttc ttcaaatagt gcttcttctg
     4441 cctttttacg atcagttatg tcaagtactg aaacaagacc agcaggctta cccttatatt
     4501 gtactgttgc acttgtaaca taaacccatt taacctgccc atctttactg ataatcctga
     4561 attcataact atctaacccc tgt
//
LOCUS       DAOSZP010000112         4580 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_15517_length_4580_cov_3.731271, whole genome shotgun sequence.
ACCESSION   DAOSZP010000112 DAOSZP010000000
VERSION     DAOSZP010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4580
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15517_length_4580_cov_3.731271"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            579..1766
                     /gene="nhaA"
                     /locus_tag="PK348_03555"
     CDS             579..1766
                     /gene="nhaA"
                     /locus_tag="PK348_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009669618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HXK65323.1"
                     /translation="MKENNQSFLHQFFAMESASGMLMIIATILAMIIANSPMKLLYTM
                     MLELRFAVSIGDFAIDKPLLLWINDGLMAIFFFMIGLELKRELVEGELSNIKKVALPA
                     IGAVGGMLVPALIYAAFNYSDPQALKGWAIPAATDIAFSLGILSLLGNRVPLSIKIFL
                     TSLAIFDDLGAIIIIAFFYTANISLTALAVSLVCIPVLLFINKKGITSKSIYVIIGIV
                     MWAAMLKSGVHATLAGVILALCIPLTSKDATSPLREFEHDVHHAVAFIILPVFAFANT
                     GIDFSKMTAQHLLHGVPIGIAAGLFIGKQAGISIFCYIAWKLGIASLPSQTNWKMLYG
                     TALLCGIGFTMSLFIGSLAFEETGSNLLFDERIGILAGSLLSGIFGYLVLHKALPEKL
                     DKA"
     gene            2048..2749
                     /locus_tag="PK348_03560"
     CDS             2048..2749
                     /locus_tag="PK348_03560"
                     /inference="COORDINATES: protein motif:HMM:NF016277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65324.1"
                     /translation="MEIPERSFIANPEECPYLYNRTKTYRYTVLYNLTPDEHNTLLAQ
                     GWRHFGYTFFQPACTSCMLCIPVRIKTFDFIPTKSQRRVLRKNANTLTRINELTLTRR
                     IYNIYEDHSISRFKELPSLQFLLSNVLDVCANTYQIEYYHNSQLFAVDYIDETREALS
                     SIYCIYHSDFAYLSPGIYSILYAIELARQKRLPYFYLGYYVPGCKVMQYKISFHPYQW
                     FNWEKQKWCDEPVIY"
     gene            complement(2752..3090)
                     /locus_tag="PK348_03565"
     CDS             complement(2752..3090)
                     /locus_tag="PK348_03565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65325.1"
                     /translation="MNSKTQFDIAINERLQSNSWDETIASAVVARVKKRNRLYIACGA
                     IAALFMIVVGISLWQGSQYPAVTTYSFIHAQANGVFKNVFPEAQSTDLTYSYIDEHVT
                     DIIDTALLER"
     gene            complement(3087..3578)
                     /locus_tag="PK348_03570"
     CDS             complement(3087..3578)
                     /locus_tag="PK348_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008590268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HXK65326.1"
                     /translation="MNDKEFAKIIGHTKSVVLQAVHRHLAAQFYEAIDDVVQETYIRA
                     YRSLVSEKFKEKSSLETWLYRIAANEAKRMNKKLLREEQKLQKLSHSMDCNPPEIQYD
                     ELSLMPVIEKLPEKYKDVMKLKALGNDETQIAGKLGIPKGTVKSRLSRGKLILYKLLQ
                     EEV"
     gene            complement(3596..4087)
                     /locus_tag="PK348_03575"
     CDS             complement(3596..4087)
                     /locus_tag="PK348_03575"
                     /inference="COORDINATES: protein motif:HMM:NF025173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HXK65327.1"
                     /translation="MKKVFMYALALTLVASVAFAQGPKGKGQCCDMPMKKGPIFGDVE
                     RLKQELGLSDAQVDKIVEINSLYEKRMLDIREKLAPKMIQLKKLLLEDEVDINAVRAK
                     LKEIGDLRIELHILRIQHVMDIEKQLNKDQLKKLKAYKQMEMQRMEKMRPHGPMGFDE
                     PMM"
BASE COUNT         1307 a          964 c          720 g         1589 t
ORIGIN      
        1 gtaatattcc atattgcaca tcttctccat tactcattag ctttgcagat tcattgtaat
       61 ccgttatcct ctttacctca tatccatacg cctttgctat tgtatcctga tatgttgtcc
      121 catctgcttt tattgctccg gcattgtacc agcgctctct ctattttttt accctaaaat
      181 tctctttttc ctgtgaaact ttagaatatc ccaatatgaa tatagttcca atgactattt
      241 ttaggatctt tactaagata tagtattaac ttatacttat ctatcatatt tttgtaaatt
      301 ttatttatgt agttcatcac ttttttatca ttataaaaaa tcctcatctt tttgttctta
      361 ttatcttcta aatatgcact tgagctatca ataaaatact ctatcaattt cattgttgct
      421 tctaaatcat aaactttgca tttatcaaaa tcaccactat aaatcgtatc atatacagta
      481 tctggtagta aaacattttt aattgcaata attatcacta taagatatga ttcaattata
      541 tcataaaagc cattacatgt aaaaggagtt gattcctcat gaaagaaaat aaccaatcat
      601 tcttacacca attctttgca atggaatctg caagcggcat gttgatgatc attgcaacca
      661 tactggctat gattattgcc aattcaccta tgaagctatt atatacaatg atgcttgaac
      721 ttcgttttgc agtatccatt ggggactttg caattgacaa accacttttg ctatggatta
      781 acgatggact catggcaatt tttttcttca tgattggcct tgaactaaaa agagaacttg
      841 ttgaagggga actatcgaac ataaaaaaag tagcactgcc tgctattggc gcagttggag
      901 gcatgctggt tcctgcttta atatatgcag cgttcaatta tagtgaccca caagcgttaa
      961 aaggttgggc aatacctgca gccaccgata ttgcattctc gttaggcatt ctttcattac
     1021 ttggtaaccg agtaccatta agcatcaaga tatttttaac ttcacttgct atttttgatg
     1081 atctgggtgc aattattatc attgcatttt tttatactgc aaacatttct ttaactgccc
     1141 ttgctgtatc gcttgtatgt attcctgtat tattgttcat caacaaaaaa ggcatcacct
     1201 caaaaagcat atatgtaatt ataggaatag taatgtgggc agcaatgctg aaatccggtg
     1261 tccatgcaac actggctggg gttatactgg cattatgtat tcctttaaca tcaaaagatg
     1321 ccacttcacc actgcgagaa tttgaacatg atgtacatca tgctgtagca tttattatat
     1381 taccagtatt tgcatttgcc aatactggaa ttgattttag caagatgaca gcacagcatc
     1441 ttctacacgg agtacccatt ggcattgcag caggtctttt tattggtaaa caggcaggca
     1501 tatctatctt ttgctacata gcctggaaac ttggtatagc atcactgcca tctcaaacca
     1561 actggaagat gctgtatgga actgcattac tatgtggcat aggtttcacc atgagtcttt
     1621 ttattggctc actggctttt gaagaaacag gcagcaatct tttatttgat gaacgcatcg
     1681 ggattctggc agggtcactg ctgtcaggga tttttgggta tcttgtgttg cataaagcac
     1741 ttcctgaaaa gttagataaa gcataaaaag tcaatgcatg taatcttcac ttgtagcgtc
     1801 tgaagaatca atttccttct ttaaaattaa gattgccatg accctcctgg gctcgcaatg
     1861 acataaatat aataacttta tagactattt tttttattct ggttggtcac agttgtggtc
     1921 aggagcaatg tgctaaatac ttaatgatta catggttaag ctaaaaattt ttttcttaaa
     1981 aaaactttat gttcagcctt ccttaaatta ttgacaattt atacattttc acaattatac
     2041 tatcagcatg gaaattccag aaagatcatt catagccaat cccgaagaat gtccctatct
     2101 ttataataga accaagacat atcgctatac cgtactgtac aatctcacac cagacgaaca
     2161 caacacactt ttagcacagg ggtggcggca ttttggttat accttttttc agccagcgtg
     2221 cacatcatgt atgctatgca tacctgtaag gattaaaaca tttgatttta tacccacaaa
     2281 aagtcagcgc agggttttac gtaaaaatgc aaacacgctt actcgcatca acgaattaac
     2341 ccttacacga agaatttaca acatatatga agaccattcc atatcccgtt ttaaagagct
     2401 accatcacta caatttttat tgtcaaatgt acttgatgta tgtgcaaaca cctaccagat
     2461 tgaatactat cacaactcac aactgtttgc cgtggactac attgatgaaa cacgtgaagc
     2521 attaagttcc atttactgta tctatcactc agactttgct tacttaagtc ctggaattta
     2581 tagtattctg tatgccattg aattagcccg gcaaaaaagg ctgccctact tttatttagg
     2641 ctactacgtt cctggatgca aggttatgca gtacaaaata tcatttcatc catatcaatg
     2701 gtttaactgg gaaaaacaaa agtggtgtga tgaaccggtt atttattaaa gctatcgctc
     2761 cagcaatgcc gtatcaataa tatctgttac atgctcatca atatagctgt atgtcaggtc
     2821 agtagattgt gcttcaggga aaacattctt aaacacacca tttgcctgtg catggataaa
     2881 gctatacgtg gtaacagccg gatactgtga cccctgccac aaagaaattc caaccacaat
     2941 catgaaaagt gcagcaatag caccacatgc aatatacaat ctatttcttt ttttgacccg
     3001 tgctactact gctgaagcaa ttgtttcatc ccacgagtta ctttgcaatc gctcattgat
     3061 tgctatatca aattgtgttt tactattcat acttcctcct gtaacaactt ataaagaatc
     3121 agtttccctc tggataagcg cgattttacc gtaccctttg gaatacccag cttccctgca
     3181 atctgtgttt catcattacc cagagctttc agtttcataa catctttata cttttcaggg
     3241 agcttttcaa taacaggcat aagcgatagt tcatcatatt gaatctcagg aggattacaa
     3301 tccatactat gtgaaagctt ttgaagtttt tgctcctctc gcaaaagctt cttattcata
     3361 cgttttgcct catttgctgc tatgcgatac aaccaggtct caagagatga cttttctttg
     3421 aatttttctg aaacaaggct tcggtaagcc cgaatatacg tttcctgtac tacatcatct
     3481 attgcctcat aaaactgtgc tgcaaggtgc ctgtgcacag cctgcagcac aactgatttt
     3541 gtgtggccaa taatttttgc aaactctttg tcattcatgt tataagcctt aacgtttaca
     3601 tcattggttc atcaaatccc attgggccat gtggacgcat tttttccatt cgttgcattt
     3661 ccatctgttt gtaagctttt agcttcttta gctggtcttt gttaagctgt ttttcaatat
     3721 ccataacatg ctgtattctc aatatatgaa gctcaatccg taaatcgcct atctctttca
     3781 gtttagctcg cactgcatta atgtcaactt catcttcaag caaaagcttt tttagctgaa
     3841 tcattttggg agctaatttt tcccgtatgt caagcatgcg cttttcatac agactattga
     3901 tttcaacaat cttatctacc tgggcatctg acaaccccaa ttcctgcttt agccgctcta
     3961 catcaccaaa gattgggcct tttttcatgg gcatatcgca acactgccct ttcccttttg
     4021 gcccctgcgc aaatgcaaca ctggcaacaa gtgtcagagc cagagcatac ataaaaacct
     4081 ttttcataca aatccccctt taagtatagt agcttctcaa attttgaaca ttagaccttt
     4141 attcaaagct tttagttcca ttttttttgt tatattcttc atttttagct ctgacaccgc
     4201 acctgcatac gggacttcat ctttggcgta gtagtatctt gctctgacac catactttca
     4261 caaagactta attcgggtaa cacaataatt attatgctct gtcatccttg ggcgatagtt
     4321 accgatgatt atacctttcc attttttctt gcttctttta ttcttttgct ctgacactgt
     4381 atacagagtg cacctgctta cacggacttt gcatattgcc gtgatgatgt tgtaatgaac
     4441 gtctcttccc gtgtgacaac cacttttgct ctgacaccat atatctttaa gatatgctct
     4501 gacactgagt tttaaatttt tttcctattt taaaaaattt tttatcactt cccatacttt
     4561 ttttcacctt catgcgttgt
//
LOCUS       DAOSZP010000113         4526 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_15716_length_4526_cov_3.899351, whole genome shotgun sequence.
ACCESSION   DAOSZP010000113 DAOSZP010000000
VERSION     DAOSZP010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4526
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15716_length_4526_cov_3.899351"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..572
                     /locus_tag="PK348_03580"
     CDS             <1..572
                     /locus_tag="PK348_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013705384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HXK65328.1"
                     /translation="GKPIYLLSDEPYRELVYDNSTVPSILSHYTHSMVVSSFSKTLSL
                     PGERIGYIAINPSMPEADIVMAGLILCNRILGFVNAPALMQRVVEKIAFDTVDVKAYS
                     TRRDMICEGLQKAGYSFVKPKGAFYVFCKSPLEDDVVFVKHLLNFNILAVPGVGFGGP
                     GFFRLAFCVPEKVIEKSMPQFKKALESLS"
     gene            583..1101
                     /gene="lepB"
                     /locus_tag="PK348_03585"
     CDS             583..1101
                     /gene="lepB"
                     /locus_tag="PK348_03585"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HXK65329.1"
                     /translation="MIEQKDSFNVFAKVLFAVLGFIVGFIAIRLFLIPVKIDNALMEP
                     TLAVNDYVWFYKTKKVDSGSIVLYKSPLSGKLVGRIIARSGETVEIINKQLYINGVKA
                     APQWAIKKSDTRIFPSRFVNRDNMNTITVPDGHVFILNDNWDTPNDSRLFGVIAIENI
                     VGKYLFKISLKK"
     gene            complement(1151..1972)
                     /locus_tag="PK348_03590"
     CDS             complement(1151..1972)
                     /locus_tag="PK348_03590"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HXK65330.1"
                     /translation="MKFRLLSLVISLFVFTEVLLADDYIEMLKNGTWEQRITAMYTLG
                     YSGNKKAFWYLVKYLDQSFQSENQSLGVRVRQAAAISLGRLKDDRAIPYLVERYKKEE
                     NTEVKRAIIFALRFFKPTPEAITVVKQGLADSNKDIKWEALMVSSVYCDSSLASDIAQ
                     LANSDDPEIKVIAAYIQYTLNQNKPENEALMRKSLTHKSPLVRYWASHFLVKSVGTPA
                     LEDIMKALEIESVWWVANEMENSIYILSRKKFNEEMVEQLGSFRFVVDKKEKQQQ"
     gene            2259..3560
                     /locus_tag="PK348_03595"
     CDS             2259..3560
                     /locus_tag="PK348_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013451013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HXK65331.1"
                     /translation="MFNKQYEAMEVKKLKELQLERLKWSLKHAYNNVPYYRQSFDKAK
                     FNPDKFSSLDDLKHVPFLMKQAMRDSYPFGLFAVPMEKVIRIHSSSGTTGNATVVGYT
                     KNDIEVWAEVMARTIAAAGGTEKDIIQVAYGYGLFTGGLGVHYGAEKLGATVIPISGG
                     NTERQLMLIKDFGTTMLACTPSYAINMADFVEKKMKDFDFKKTKLKAGIFGAEPWTES
                     MRKEIESRMGIDAYDIYGLSEVIGPGVSCECSEKSGLHVFEDHFYVEVIDPETGEVLP
                     EGEKGEIVYTSLTKEACPVIRYRSRDITRIYRDACKCGRTLIKMEKVTGRSDDMLIIR
                     GVNVFPMQIEAVLMEMEGTEPHYQIIVDRKGALDDVEIMVEVNEKIFTDELGALKGLA
                     ERIEQRIRSALGISAKITLVEPSTLQRSEGKAKRVIDKRQL"
     gene            3589..4020
                     /locus_tag="PK348_03600"
     CDS             3589..4020
                     /locus_tag="PK348_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HXK65332.1"
                     /translation="MNVTQLSLFLENKPGHLLNALKVLADENVNILTLTIAEVADFGI
                     VRMIVNKPQKALEALRQHKFVCSPTDVLAIELDDKPGSLLKALEIFSKRNLNIEYMYA
                     FTEKRGDKAVMIFKFADIETAKKALLEEGFNVVKKIDLIGE"
     gene            4025..>4526
                     /locus_tag="PK348_03605"
     CDS             4025..>4526
                     /locus_tag="PK348_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019224841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HXK65333.1"
                     /translation="MQKKVMLGNEAIARGAYEAGCIVAAAYPGTPSTEILETIAAEYP
                     DIKAQWSPNEKVALEVVAGASVAGARAMAAMKHVGLNVAADPLFTFSYTGVNGGFVII
                     NADDPGAWSSQNEQDNRHYARAAKLPMIEPTSPEEARKYTKLAFELSEQFDRPVIVRI
                     TTRIAHS"
BASE COUNT         1390 a          795 c          997 g         1344 t
ORIGIN      
        1 ttggaaaacc catttacctg ctttcggatg aaccataccg cgaactggta tatgacaata
       61 gtacagtgcc aagcatcctt tcccattaca cgcattcaat ggttgtttca tctttttcaa
      121 aaacattatc acttcccggt gaacgaatag gatacattgc aattaatcct tccatgccag
      181 aagctgatat tgttatggcg ggacttatat tatgtaacag gatattaggg tttgttaatg
      241 cgcctgccct catgcagcgg gttgtggaaa aaattgcatt tgacaccgtt gatgtaaaag
      301 catattcaac acggcgggat atgatttgtg agggattgca aaaggcagga tattcctttg
      361 ttaagccaaa gggggcattt tatgtgttct gcaaatctcc actggaggat gatgtggtgt
      421 ttgttaaaca tctgcttaat tttaatatac tggcagttcc tggtgttgga tttggaggcc
      481 ccgggttttt tagattagct ttctgcgttc cagaaaaggt tattgaaaaa tccatgccgc
      541 aatttaaaaa agcccttgaa tcactttctt aaaaaaatgg ccatgattga acaaaaagat
      601 tcatttaatg tttttgctaa agttttgttt gcagtgttag ggtttatagt tgggtttatt
      661 gctatacgcc tttttcttat tccggtaaag attgacaacg ccttaatgga acccactctt
      721 gcagtcaatg attatgtgtg gttttataaa acaaaaaaag ttgattcagg ttcgatagtt
      781 ctttacaaaa gccctctttc aggaaagctt gtaggcagga taattgccag atctggtgaa
      841 acagtagaga ttatcaataa acagctttat atcaatggtg taaaagcagc acctcagtgg
      901 gcaataaaaa aaagtgatac acggatattc ccatccagat tcgttaaccg ggacaatatg
      961 aacacgataa cagtgcctga tgggcatgtt tttatattaa acgataactg ggatactccc
     1021 aatgatagca ggctatttgg tgtgattgca attgaaaata ttgttgggaa atacttattc
     1081 aagatttcac ttaaaaagtg aaatgtatag tgattattag actgccgatg gtagtctggt
     1141 gcagaatttt ttactgctgc tgtttttctt ttttatccac aacaaatctg aaagaaccta
     1201 actgctcaac catttcttcg ttaaatttct tacgggatag aatatagata ctattctcca
     1261 tttcatttgc aacccaccat actgattcaa tttcaagagc tttcattata tcttcaagtg
     1321 caggagtacc tactgattta accagaaaat gtgatgccca gtagcgtacc agtgggcttt
     1381 tatgtgttaa tgatttgcgc ataagcgctt cattttcggg cttattctgg ttaagcgtgt
     1441 actggatata tgcagcaatt actttaattt cggggtcatc gctgtttgca agctgagcaa
     1501 tgtctgatgc taaggaacta tcacaatata cgcttgatac catgagtgct tcccatttaa
     1561 tgtctttatt ggaatcggca aggccttgct ttaccacagt tatagcctca ggagtgggct
     1621 taaaaaaacg aagagcaaaa ataatggcgc gcttaacttc agtgttttcc tcttttttgt
     1681 agcgttcaac taaatagggt attgccctgt catctttaag ccgtcccaat gaaatagctg
     1741 ctgcctggcg tacccgtact cctaaagact ggttttctga ctggaatgat tggtcaagat
     1801 atttaactaa gtaccagaaa gcctttttgt tgccggaata gcctaatgtg tacattgcag
     1861 taattcgctg ttcccaggta ccgtttttca gcatttcgat ataatcatct gccagaagta
     1921 cctctgtaaa aacaaataat gaaataacaa gtgatagtaa gcgaaatttc atggtatagt
     1981 ccccttatta tgattgaatg tattccgtac acatatatac aatttctttt gtttgcacac
     2041 tggagtgcaa tatcagtaac tatcgccctt aaatatagaa tatgcgatgg tgaatgtgca
     2101 ctgttaattc aaacatacca ggggtataat aaaaaactca attaaaattt aatttttttt
     2161 ccttgcatat acaaggttat tgacaatttt aataaatata aatacaattt actttcttaa
     2221 aaattaaaaa atatagagcc ataaaaaagg agccggcaat gtttaataaa cagtatgaag
     2281 caatggaagt taaaaagtta aaggaattgc agcttgagcg attgaaatgg tcgctgaagc
     2341 atgcgtataa caatgtcccc tattatcgcc agtcatttga taaggctaaa ttcaatcctg
     2401 ataaattctc atctcttgat gatctgaaac atgtgccatt tttaatgaag caggccatgc
     2461 gtgatagcta tccgtttggc ctttttgctg ttcctatgga aaaagttata cgcattcatt
     2521 catcatctgg cactactggc aatgctacag tagttggata cacaaaaaat gacattgagg
     2581 tgtgggctga ggtgatggca cgcaccattg ctgcggctgg tggtactgag aaggacatta
     2641 ttcaggtggc ttacggctat ggtcttttta ctggtggtct gggtgtgcac tatggtgcag
     2701 aaaagctggg agctaccgtt atcccaattt caggcggcaa tactgagcga caactgatgt
     2761 taataaaaga ttttggtaca acgatgctgg catgtacacc ttcgtatgca atcaacatgg
     2821 ctgactttgt tgaaaaaaag atgaaggatt ttgactttaa aaagaccaaa ctgaaagcag
     2881 gtatttttgg tgctgaacca tggactgaaa gcatgcgaaa agagattgaa agtcgtatgg
     2941 gtattgatgc ctacgatatt tatggcctga gcgaagtgat tggacccggc gtatcgtgcg
     3001 agtgcagcga aaagtccgga ttacatgtgt ttgaagatca tttttatgtt gaagtgatag
     3061 atcctgaaac aggtgaggtg ttgccggaag gggaaaaagg tgagattgtg tatacatcgc
     3121 ttaccaagga agcgtgccct gttatacggt atcgttcacg tgatattacc aggatttatc
     3181 gtgatgcgtg caagtgcggg cgtactttaa taaagatgga aaaggttaca ggccgaagcg
     3241 atgatatgct tatcatacgc ggtgttaacg tattcccgat gcagatagaa gcagtgctca
     3301 tggaaatgga aggtaccgaa ccacactatc agattattgt agaccgcaaa ggtgcactgg
     3361 atgacgtgga aattatggtt gaggtaaatg aaaagatatt taccgatgaa ttgggtgcct
     3421 tgaagggact ggcagaaaga attgagcagc gcatacgaag tgctttaggt ataagtgcaa
     3481 agattacact ggttgagcca agcacgttgc agcgcagtga aggcaaagca aaaagagtaa
     3541 ttgataagcg acagctctaa taaaaagatt aatgaatgga ggaaaacaat gaatgttaca
     3601 caattatcgc tttttcttga aaacaaacca gggcatctac ttaatgcttt gaaagtactt
     3661 gcagatgaaa atgttaatat actcacactg accattgcgg aagtagctga ttttggtata
     3721 gtcagaatga ttgtcaacaa accacagaaa gcactggaag cacttcggca gcataaattt
     3781 gtttgttcgc caactgatgt actggcaatt gaactcgatg acaaacctgg ttcattgctg
     3841 aaagcactgg aaatattttc caaacgcaat ttgaatattg agtatatgta tgcatttact
     3901 gaaaaacgag gtgataaagc cgtaatgata tttaagtttg ctgatattga aacagcaaag
     3961 aaagctcttc ttgaagaagg atttaatgta gtaaaaaaga ttgatctgat tggagaataa
     4021 aacaatgcaa aaaaaggtaa tgctgggtaa tgaagctatt gcccgtggtg cgtatgaagc
     4081 cgggtgtata gttgctgctg cgtatcccgg tacgcccagt acagaaattc tggaaacaat
     4141 agctgcagaa tatccggata ttaaagcaca gtggtcaccc aacgaaaagg tggcattaga
     4201 ggttgtagcg ggtgcttcgg ttgccggtgc ccgtgcaatg gcagccatga aacacgtggg
     4261 attaaatgta gcagctgacc cactttttac attttcatac accggggtca atggtggttt
     4321 cgtcattatt aatgcggatg accccggagc atggagttca cagaatgagc aggacaacag
     4381 acattatgcc cgtgctgcaa aattacccat gattgagcca acaagccctg aagaagcgcg
     4441 taaatacact aaattagcgt ttgaattatc tgaacagttt gatcgtcctg ttatcgtacg
     4501 cattactacc cgtattgctc attctc
//
LOCUS       DAOSZP010000114         4510 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_15791_length_4510_cov_3.267116, whole genome shotgun sequence.
ACCESSION   DAOSZP010000114 DAOSZP010000000
VERSION     DAOSZP010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4510
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15791_length_4510_cov_3.267116"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..513
                     /locus_tag="PK348_03610"
     CDS             <1..513
                     /locus_tag="PK348_03610"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HXK65334.1"
                     /translation="RINADYALLMSSVYKECSTNNIQQALLLCKRVPGPISELIHNAI
                     TFASTKKEREGLIAEQVYAAQKAIEKHIGIIATIATIAPMFGLLGTVTGMMKSFSALS
                     KVGSVAQDLLAYGIAEALITTALGLLVAIPSWIFYNYLVSRVERFNKDLEYVANTLLD
                     FPLCSIEQKQ"
     gene            553..978
                     /locus_tag="PK348_03615"
     CDS             553..978
                     /locus_tag="PK348_03615"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HXK65335.1"
                     /translation="MDQGLKTTIKVKSKLGYRSLIDITSLVDMTFLLVAFFMVTSSFG
                     SLSSITVHLPKAVQSGQMQHATMVISINEKNEVFVNDVKYPLGDLLQEFRKRKESVKD
                     KVVIIRGDRDANYETIITVMDLLNQAGIPRFTLATVKPK"
     gene            1058..>2815
                     /gene="bamA"
                     /locus_tag="PK348_03620"
     CDS             1058..>2815
                     /gene="bamA"
                     /locus_tag="PK348_03620"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HXK65336.1"
                     /translation="MKKAGIFISVIVIVALFNGGFGQPSKYEGKIVKKIEFSGLLDVD
                     EEDLLDVMTTEVGYPLRAVEIREDIKKIFKTGKFENIIVETEEYQDGVAVRFVCTERP
                     AVNKIIFKGVEEVNETTLKEELPIKEGDVYRKDKVEAGLIKIRNKYETEGLFNAIVKY
                     KIEEAKDEKNAVNVIFIVDEGEDIKVQKIAIVGNTIVPLSTLKGLLQTEEEGLFADAK
                     FNKATYEDDKAKILAYYRELGYLDADIVEDTVEYRWLDPETQEKRGIYITIKLREGER
                     YYFDGYEVSGNEVFSTEDITSQFEQTRIGDPFNDTLYQKDRQMISFTYATKGYIFARI
                     IPERKITEKEVDVDGKKEKRKYVFTHFHIKEGSQAYIENIIIKGNQKTKDKVIRRELV
                     VYEGELFNSYKMQLSRERVYNLGFFKEVNFDVRPGSKEGYMNLIVDVVEQPSGTISLG
                     GGWGSMTGFSIFADVAENNFMGNGQRVSLRFEYGPLRNSITLGFNEPWLFDKPIGLNL
                     SVFYMLSTIEQSSIFTNSNQKAKHQTQQYGYSIGLNYRFWYYYGIGSVWYHAMKEVIN
                     ASGNSTDTVFLEQAQGLQEK"
     assembly_gap    2816..2825
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2826..3585
                     /locus_tag="PK348_03625"
     CDS             <2826..3585
                     /locus_tag="PK348_03625"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HXK65337.1"
                     /translation="LTLYAYRDSKDNYLNPTRGWRAEFSASFTGGYIIRGDDHWVQYS
                     PDLYWYFSPFHLPFLKTHPCVIELRFNGTFITPPWFKNKMYDVQNPDKNPWLESEDRL
                     YIGGPETLRGWEILDFDFPDSWRYGLFHRILYGAEFRIPIHPTFLWFVFFFDAGSVWT
                     DSFWEKSLEQSTRDIINEDKANGLLYDIRDINKIDYMSYFKYSYGFGFKIQIPMMPLR
                     FWFGRKLAWVGRDEGYFKPLSGFQFQFGIGDMRF"
     gene            3588..4031
                     /locus_tag="PK348_03630"
     CDS             3588..4031
                     /locus_tag="PK348_03630"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HXK65338.1"
                     /translation="MKRFVLFILCLLIVSCQTVDREDSLTIRYVQLPILYNYMIQTSP
                     DALSLQKRYNELKAQLDVPSVSDAERKDLAVKLQSIADQMDKQKKIFLTDIQQAIATV
                     AKRKGYQIVLGGGDTVVYAKDGYDITSEILKELAALRLQKSPAAR"
     gene            4256..>4510
                     /locus_tag="PK348_03635"
     CDS             4256..>4510
                     /locus_tag="PK348_03635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65339.1"
                     /translation="MHILKPVINIVLVFLLSCSEYGYYQKKGQELLKHYKGNYSIVIS
                     KKSFMLFVVSREGIVAKYRIGYGLNPDKKPKLYANDNRTPE"
BASE COUNT         1437 a          779 c         1024 g         1260 t
ORIGIN      
        1 agaataaatg ccgattatgc actgctgatg agcagtgtat ataaagaatg cagcacaaac
       61 aatatccagc aggcattgct gttgtgcaaa agggtgcctg gccccataag tgagcttatc
      121 cacaatgcaa taacctttgc cagcacaaaa aaggaacgcg aaggacttat tgccgagcag
      181 gtatatgcag cacaaaaagc tattgaaaag catataggca ttatagcaac tatcgccacc
      241 atagccccaa tgtttggctt gcttggaact gttaccggta tgatgaaatc gttcagtgcc
      301 ttatcaaagg tgggcagtgt ggcacaggat ttgctggcat atggtattgc cgaagcgctt
      361 atcacaacag cattgggttt gcttgtagca ataccgtcat ggatttttta taattatctg
      421 gtcagccgtg ttgaacggtt taataaagat cttgaatacg tagcaaatac gctgctggac
      481 ttcccattat gttcaattga acagaaacaa taatgtcagt actgcgataa aaaaggatgc
      541 attaaagcgc atatggatca ggggttaaaa accacaataa aagtaaaaag taaattaggg
      601 tacagatcct taattgatat aacgtcactg gttgacatga catttttact ggtagcattt
      661 tttatggtga catcaagttt tgggtcacta tcgtccatta ccgtacacct gcccaaagca
      721 gttcaatcag ggcagatgca gcatgccact atggttatca gtataaatga aaaaaatgag
      781 gtatttgtta atgatgtaaa atatccactg ggggatttgt tgcaggaatt caggaaaaga
      841 aaggaatcgg ttaaagacaa agttgttatt atccgcggtg atagggatgc aaattatgaa
      901 actatcataa ccgtgatgga tctgctcaat caggcaggca taccgcggtt tacgctggca
      961 actgttaaac ccaaatgaaa actgcatatg aaatatacta aacagtaccg tgaatggtaa
     1021 atgctgtggt taaaaaagaa taaaggaatt ggacaggatg aagaaagcag ggatttttat
     1081 ttcagtaatt gttattgtag cgctatttaa cggtggtttt ggacagccct caaaatatga
     1141 agggaagata gttaaaaaga tagagttttc cgggctttta gatgttgatg aagaagacct
     1201 tctggatgtt atgaccactg aagttggcta tcccctgcga gcggtggaaa tacgtgaaga
     1261 tataaagaaa atatttaaaa ccggaaagtt tgaaaatatt attgttgaaa ccgaagagta
     1321 tcaggatggc gttgctgtcc gttttgtatg tactgagcgc cctgcagtta ataaaataat
     1381 atttaaaggc gttgaagaag tcaatgaaac aaccttaaaa gaagaattgc ccatcaagga
     1441 aggggatgta taccgcaaag ataaggttga ggcaggcctc attaaaatcc gcaataaata
     1501 tgaaacagaa ggcctgttca atgcaattgt gaaatacaaa attgaagagg caaaagatga
     1561 aaagaatgca gtcaatgtca tatttattgt ggatgaaggg gaggatatca aagtacagaa
     1621 aatagccata gtaggcaaca ctatcgttcc gttaagtaca ttaaaagggt tattgcaaac
     1681 cgaagaggag ggcctctttg ccgatgcaaa attcaataaa gccacctatg aggatgataa
     1741 agcaaaaata ctggcgtatt accgtgagtt ggggtatctg gatgctgaca ttgttgaaga
     1801 tactgtggag taccggtggt tagatccaga aacacaggaa aagcgcggta tatacataac
     1861 aataaagttg cgtgagggtg agcgatatta ctttgatggc tatgaagtat caggcaacga
     1921 ggtattttcc actgaggaca ttacttcaca gtttgaacaa accagaatag gtgatccatt
     1981 taatgacacc ctttatcaga aagaccgcca gatgataagt tttacctatg caacaaaagg
     2041 gtatatcttt gcccgcataa tcccggaacg caaaataacc gaaaaagaag tggatgttga
     2101 tggaaaaaaa gaaaaacgca agtatgtctt tacccatttc catattaaag aaggctctca
     2161 ggcgtatata gaaaatatca tcatcaaagg taatcaaaaa accaaggata aggttattcg
     2221 ccgcgaactg gttgtctacg aaggtgagct tttcaactca tacaagatgc agctttcgcg
     2281 ggagcgtgta tacaatttag ggttcttcaa agaagttaac tttgatgttc ggcctggctc
     2341 aaaagaaggg tacatgaacc tcatcgtaga tgttgtggaa cagccatcag gaacaatatc
     2401 acttggtggg ggctggggtt ccatgacggg tttttcaatc tttgctgatg tagctgaaaa
     2461 taactttatg ggcaatggtc agcgtgtttc acttcgcttt gagtatggtc cgctgcgaaa
     2521 ttctattacg cttggcttca atgaaccatg gctttttgat aagcccattg gtcttaatct
     2581 ctctgttttc tatatgctat caactatcga acagagctct atttttacaa attccaatca
     2641 aaaagcaaaa caccaaacac agcaatatgg ttattccata gggttaaatt acagattctg
     2701 gtattactat ggcataggct ctgtgtggta ccatgccatg aaggaagtta ttaatgcatc
     2761 cggtaacagt accgatactg tttttctgga acaggctcag gggttacagg aaaagnnnnn
     2821 nnnnnattaa ccctgtatgc ataccgtgat tcaaaagaca attatcttaa tcctacacgt
     2881 gggtggcggg ctgagttttc tgcatccttt actggtggct atatcattcg tggtgatgat
     2941 cactgggtgc agtattcacc tgatctgtac tggtattttt cgccatttca tctgcctttc
     3001 ctgaaaacac atccatgcgt tattgagtta cggtttaacg gtacatttat aactccgccg
     3061 tggtttaaaa ataagatgta tgatgtgcaa aatcctgaca aaaatccctg gcttgaatcg
     3121 gaagacaggc tgtatatagg cggccctgag acattgcgtg gatgggaaat cctggatttt
     3181 gattttcccg attcctggcg ttatgggctt ttccacagaa tattatatgg tgctgaattt
     3241 agaataccca tccatccaac gttcttgtgg tttgtattct tctttgatgc tggttcggtg
     3301 tggaccgata gtttctggga aaagagcctt gagcaatcaa ccagggacat catcaatgaa
     3361 gacaaagcca atggattgct ctatgatatc agggatataa ataagattga ctatatgtcg
     3421 tatttcaagt attcgtatgg atttgggttt aagatacaga tacccatgat gccgctgaga
     3481 ttctggtttg ggcgcaaatt agcatgggtt ggcagggatg aaggctattt taaaccattg
     3541 agtggcttcc agttccaatt tggcattgga gatatgcgat tctgataatg aaacggtttg
     3601 ttttatttat actgtgttta ctgatagtgt catgccagac agttgacagg gaggacagcc
     3661 ttacaataag gtatgttcag ttacccatat tgtataatta tatgattcag acatcgccgg
     3721 atgctttatc attacaaaag cgttataatg agttgaaagc acagcttgat gtcccatcgg
     3781 taagtgatgc cgaaagaaaa gaccttgcag taaagcttca atcaattgca gatcaaatgg
     3841 ataagcagaa aaaaattttt ttaacggaca ttcagcaggc gatagcaact gtagcaaaac
     3901 gtaaaggata ccagatagtc cttggtggcg gtgacaccgt tgtgtatgca aaagacgggt
     3961 atgatattac cagtgaaata cttaaagagc ttgcagcatt gcgcttgcag aaatcacctg
     4021 cagcacggtg atgttttaat tcaactttgt tgggacttgc aatgacgaca gtagtacgtc
     4081 actgagagcg aagcgaagca gtctcaggtc aactgcaatg gatgtatgtc aaacggcgag
     4141 attcttcggc cgccccaata aagtcagggc gtcttcagaa tgacagagtg ctattgtatt
     4201 gggacttgta atgacatcta catataattc catgcaaaag gagtatttta tttgtatgca
     4261 tatcctgaaa cctgtaatca atatagtatt agtattcctt ctttcctgta gtgaatatgg
     4321 atactatcag aaaaaagggc aggaactttt aaaacattat aagggtaatt acagcatcgt
     4381 tatcagtaaa aaatcattca tgttatttgt tgtcagccgg gagggtattg ttgcaaaata
     4441 ccgcatagga tatggattaa atcctgataa aaagccaaag ctgtatgcaa atgacaaccg
     4501 tacaccagag
//
LOCUS       DAOSZP010000115         4458 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_16003_length_4458_cov_3.610720, whole genome shotgun sequence.
ACCESSION   DAOSZP010000115 DAOSZP010000000
VERSION     DAOSZP010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4458)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4458)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4458
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16003_length_4458_cov_3.610720"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..908
                     /locus_tag="PK348_03640"
     CDS             <1..908
                     /locus_tag="PK348_03640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65340.1"
                     /translation="EKTPKGYTLKINIAKKQYIAFNPKGEIIYSDDNKTKPFHTMKTI
                     VFIGLESNTLTRNHPYQLLKIQNNALIISYNAGEGKRQYLFFPSDTLLDMVPCITEFF
                     NDTASFVSYVKKGNVPDYIIVNSTFSNNDITFISSISPSSRLEIVNTTSAVTIDDTKR
                     AIDQMNNVDINLLSQNPVFLARIHLRNLDLVRVKQIILDMDITESDAQYIIAFLSTMI
                     KNSDKEELQKHVEKLKELKKTYEFYFHLITHNDEKIQEFITQASKNDLITFQTLIQKA
                     KLLFPTKEASLRFVEYENLIWEKQQ"
     gene            905..1948
                     /locus_tag="PK348_03645"
     CDS             905..1948
                     /locus_tag="PK348_03645"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HXK65341.1"
                     /translation="MKHYTKLPIVFLLIITVYSFVYAQLPIEVYFTTPEKEEQTTAIQ
                     KALLQKIHSTKTSIYAALFEITDPLIIDALCKAKSNGIDVRLVLESDRLKEKVKTKLE
                     EANIPLRLDERKGFMHNKFFIFDNKAVWTGSYNITEREAKHNNNNAIYIENEEIASIY
                     LAEFNEMFVQAIFGNRTEYTPFPFLSNKYYVKINDIDINVYFSPDDDIERIITKRIEK
                     AKKSILFLAFSFTSDAIGEAIIAKHKQGIAVKGVFEKRDIKNVNSEYIKLKVEGLDVL
                     YDANPKTMHHKVIIIDDEWVITGSYNFSKNARKRNDENCIMIRSSEIAKLYAQEFEKI
                     YSQAYRAKTKKKK"
     gene            2028..2483
                     /locus_tag="PK348_03650"
     CDS             2028..2483
                     /locus_tag="PK348_03650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65342.1"
                     /translation="MKIKSALINRFVELIEENHQIITEQFMNNLLRNPDTQAFRKLDR
                     QLIYESADNLYRELSKWIARDYPKEEIARYYRRIGHERFEQGIPVSQACKALILQKRH
                     LWLFVLDKLYDDTTAYKEALALNNRVVLYFDRATFAMLQGYEDMLYKKL"
     gene            complement(2478..3398)
                     /locus_tag="PK348_03655"
     CDS             complement(2478..3398)
                     /locus_tag="PK348_03655"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXK65343.1"
                     /translation="MHRRKSIVLIIASLFVYVSCSSSLLNFFRWYERSKAGLTEKTVM
                     IDGHTVHYLEGGKGDTVLCIHGFGGTMDHWTRFARYVTDAYRVVALDLPGFGDSSFKE
                     GGDYSIAKQVERVHEFAHTLKLQKFHIVGNSMGGYIAGYYAITYPDDVITLGLFAAAG
                     VKSPNESELAKELKKGNNPLIVEDVESYDRLMEFVFYKPIWLPGFAKQYFATQAVAKK
                     THFQYIFKQIHDVTLLEKYLGNIHAPTLLLWGENDRVLDVSGAYVFKEKIRNAQLVVF
                     KECGHLPMIEKPEESATVYKRFLKGQKVEL"
     gene            complement(3463..>4458)
                     /locus_tag="PK348_03660"
     CDS             complement(3463..>4458)
                     /locus_tag="PK348_03660"
                     /inference="COORDINATES: protein motif:HMM:NF019869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HXK65344.1"
                     /translation="FRFTLAIFAAQGRDIILSEKRIEGYSAFCNKIWNSTRFILMNLG
                     NDFKAEAIDINSLDMFDKWILHAFNQTVKKTTQALVEYRFNDAAQTLYDFWWHEFCDW
                     YIELSKPRIYAGDSSTGTIAKQVLYFVLHEYLKLLHPFMPFITEEIWSNITSGEGRIV
                     VAPWPQYDERFNFEHEFNNAELFKEIVYKIRNIRGEMNVPPDKKAHVVIKAGKKIEQF
                     IQQQEHNLKILAKLEAVECNADYTPQKTDASAVLPECELYMPLKDLIDLDKEKARLDK
                     EIARIQGELDKVMKKLNDSTFLEKAPAHIIEKEQNKKAELEEVLGKLTTGREKLG"
BASE COUNT         1471 a          882 c          731 g         1374 t
ORIGIN      
        1 aggaaaaaac accaaaaggc tacactttaa aaataaacat tgcaaaaaag cagtatattg
       61 ccttcaatcc aaaaggggaa attatctatt ccgatgataa taaaacaaaa cctttccata
      121 caatgaaaac aattgttttc ataggacttg aatcaaacac tttaaccaga aatcacccct
      181 atcagttatt aaaaattcaa aataacgctt taattatttc ctacaatgct ggtgaaggta
      241 aacgacaata cctttttttc ccctctgata ccctccttga tatggtaccc tgtattacgg
      301 aattttttaa tgatacggct tcatttgtca gctatgtaaa aaagggcaat gtccctgatt
      361 atataattgt caatagcaca ttcagtaaca acgatattac atttatatct tctatatctc
      421 cttcatctcg tctggagata gttaatacaa cttctgctgt tactattgat gataccaaac
      481 gtgccattga ccagatgaat aatgttgata ttaatttgct ctcgcaaaat cctgtatttc
      541 tggcacgaat tcatctgcgt aatcttgatt tagtccgcgt aaaacaaatt atacttgata
      601 tggacattac agaatcagat gcacaatata tcattgcatt tctttctaca atgattaaaa
      661 attcagacaa agaagaactt cagaagcatg ttgaaaaact caaagaattg aaaaaaacat
      721 acgaatttta ttttcatctg ataacacaca atgatgaaaa aatacaggag ttcataacac
      781 aggccagcaa aaatgacctg ataacattcc agacgttaat acaaaaggct aaattgcttt
      841 tccccacaaa agaagccagt ttacgatttg ttgaatatga aaacttgatc tgggaaaaac
      901 agcaatgaag cattatacca aactacccat tgtattttta ctgataatta cagtatattc
      961 gtttgtttac gctcagttac ccattgaagt atactttacc acgccagaaa aagaagaaca
     1021 gacaactgct atccagaaag cattattaca gaaaatacat tctaccaaaa catctatcta
     1081 tgctgctctt tttgaaatca ctgatccatt aattattgat gctctctgta aggctaagag
     1141 taatggtatt gatgtcaggt tagtactgga atcagacaga ttaaaagaaa aggttaaaac
     1201 aaaacttgaa gaagccaaca tacctctgcg actggatgag cgaaaaggat ttatgcataa
     1261 taaattcttt atttttgaca ataaagccgt gtggaccggg tcatataaca ttactgaacg
     1321 cgaggcaaaa cacaacaaca ataatgccat atatattgaa aatgaagaga tagcatccat
     1381 atacctggca gaatttaacg aaatgtttgt acaggctata tttggcaatc gcactgaata
     1441 tacccctttc ccttttctat caaataaata ttatgtcaaa atcaatgata tagacattaa
     1501 tgtgtatttt tcaccagatg atgatattga gcgcatcatt acaaagcgta tagaaaaagc
     1561 aaaaaaatcc attttatttt tagctttttc atttaccagt gatgctatag gtgaagcaat
     1621 tattgctaaa cacaagcagg gtattgctgt taaaggtgtt tttgaaaaac gggatattaa
     1681 aaatgtcaac agtgaataca ttaaattaaa agttgaaggg cttgatgttc tctatgatgc
     1741 taatcctaaa acgatgcatc ataaggtaat cattattgat gatgaatggg ttattactgg
     1801 ctcatataat ttttcaaaaa atgccaggaa aagaaatgat gaaaattgca tcatgatcag
     1861 atcatctgaa atagcaaaat tgtatgcaca ggaatttgaa aaaatctact cacaggctta
     1921 tagagccaaa accaaaaaaa agaagtaatg taaaaaaata ctttacatat agataatcgt
     1981 aatatttttt atcattatat tagactatat tatcataata caatactatg aaaatcaaaa
     2041 gtgcactgat taacaggttt gttgagctta ttgaagaaaa ccatcagatt attactgaac
     2101 agtttatgaa caatcttttg cgtaatcctg atacccaagc attcaggaag ttagaccgac
     2161 agctcattta tgaatctgca gacaacctgt accgtgaatt atcaaagtgg atagccagag
     2221 attatcccaa agaagaaatt gcccgctact atcgaaggat tgggcacgag cgctttgaac
     2281 agggaattcc tgtttctcaa gcatgtaaag cattaatttt acagaaacga catctgtggc
     2341 tttttgtact ggataaatta tatgatgaca ccactgccta taaggaagca ctggcgttaa
     2401 acaaccgtgt ggtgctgtat tttgatcgtg ctacgtttgc catgcttcag ggttatgaag
     2461 atatgcttta caaaaaatta taactccacc ttttgaccct ttaaaaacct tttatataca
     2521 gttgcagatt cttcaggttt ttcaatcatt gggagatgac cacattcttt aaatactacc
     2581 agttgcgcat tacgtatttt ttctttaaag acatacgcgc ctgatacatc cagcacccta
     2641 tcattttcac cccataatag taacgttggt gcatgtatat tacccagata tttttcaagc
     2701 agtgtaacat catgaatctg cttaaaaata tactgaaaat gagttttctt tgccactgcc
     2761 tgtgtggcaa agtactgttt tgcaaaccct ggcagccata ttggcttata gaacacaaat
     2821 tccatgagtc tatcatagct ttccacatct tcaacgataa gagggttgtt tccttttttc
     2881 agctcttttg ccagttcact ttcattgggg ctttttacac cggctgctgc aaacaatccc
     2941 aatgtaataa catcgtctgg ataggtaatt gcatagtagc cggcaatata cccacccatt
     3001 gagttcccaa caatgtgaaa cttctgcaat ttcagtgtgt gtgcaaattc gtgaactctt
     3061 tctacctgtt ttgctattga atagtcaccg ccttctttaa aggaactatc gccaaaacct
     3121 ggaaggtcaa gcgccacaac tctgtatgca tccgtcacat accgtgcaaa gcgcgtccag
     3181 tgatccatgg tgccaccaaa accatgaatg caaagaacag tgtcaccttt gccaccctca
     3241 aggtaatgga ctgtgtggcc atcaatcata actgttttct cagttaagcc agctttagag
     3301 cgttcgtacc atcggaaaaa gttcagtaaa gaagaagaac aggaaacata aacaaaaagt
     3361 gatgcaataa ttaatacaat actctttctt ctatgcatag tgaacctcca ctaactatga
     3421 gtataagcac ctctaccaac aatgatagag gtgctggtta tattacccta atttttccct
     3481 gcctgtggtg agcttcccaa gcacctcttc aagttctgcc tttttattct gttctttttc
     3541 aataatatgc gctggtgctt tttcaagaaa tgtgctgtcg tttaattttt tcattacttt
     3601 atcaagctca ccctgtatac gtgcaatttc tttgtcaagg cgtgcttttt ccttatcaag
     3661 atcaattaaa tccttaagtg gcatataaag ctcacattcc ggaagcaccg ctgaggcatc
     3721 tgttttctgc ggtgtgtagt cagcattgca ttcaactgct tcaagctttg ctaaaatttt
     3781 caggttatgc tcctgctgtt gtataaactg ttcaattttt tttccggcct ttataactac
     3841 atgagccttt ttatcaggtg gtacattcat ttcaccgcgg atatttcgga ttttatatac
     3901 tatctcctta aacaattcag cattattaaa ctcatgttca aaattaaaac gttcatcata
     3961 ctgaggccat ggtgccacca caatgcgccc ttctccgctt gtaatattac tccatatctc
     4021 ttcggtaata aacggcataa acggatgcag caatttaagg tattcatgca gcacaaaata
     4081 cagtacctgt ttggcgatag ttcctgttga agagtccccc gcataaatac gcggctttga
     4141 cagctcaata taccagtcgc agaattcatg ccaccagaaa tcatacaacg tttgtgctgc
     4201 atcattgaat cgatactcta caagcgcctg tgttgtcttc ttcacggtct gattgaatgc
     4261 atgcagtatc cacttatcaa acatgtccag tgaattgatg tcaatggctt cagccttgaa
     4321 atcattgcct aaattcatca gaataaaacg ggtgctattc catatcttat tacagaatgc
     4381 gctgtagcct tcaatacgct tttcagatag aattatatcc ctgccctgcg cggcaaatat
     4441 agctaaggta aacctgaa
//
LOCUS       DAOSZP010000116         4457 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_16010_length_4457_cov_3.211268, whole genome shotgun sequence.
ACCESSION   DAOSZP010000116 DAOSZP010000000
VERSION     DAOSZP010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4457
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16010_length_4457_cov_3.211268"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1007
                     /gene="dprA"
                     /locus_tag="PK348_03665"
     CDS             <1..1007
                     /gene="dprA"
                     /locus_tag="PK348_03665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HXK65345.1"
                     /translation="IYNEYVLPEERKVQEYYTSWYSMSPLEEAEKIIELCNKMNVNMI
                     SYWDEDYPALLKEISYPPAVLYVRGNLPNKLSLAIVGTRNDDTQSGSIAEKLAGILAQ
                     YDIAIVSGLAIGIDRRAHIGALKAGGSTIGVMANGIDMLYPASNRDIYTDIINSGNGC
                     VLSEYPPKVRITKWTFVRRNRIISGLSKGVIVIKAGEKSGALITAQYALEHNRELFVC
                     TGHSFDEGYKGCYKLLKEGAHPVFNEQDILEILSVDKLYRQPTLFEGMEFNKVTSNTH
                     FAAYEDDDIVKTLKQGSMDIDSLIRAVGKNSGEVLEKLTTMELEGVIIRNGNLISLNK
                     "
     gene            1043..1627
                     /locus_tag="PK348_03670"
     CDS             1043..1627
                     /locus_tag="PK348_03670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65346.1"
                     /translation="MLALLWVISFIQIFSVPPYIGDIFEIYKSGYFKELERNMYIITD
                     SFLHIKTMTKPEYAWIYLDDLQKRSGISIAVYDAQGNLIKAPGMSEIVNNTEIMNVCN
                     DIKPQAQFTVSGRLYHGIIPVYRKSECNFCHQPSQKPLLGVITYAIPFDGYIYYTSER
                     IILFTIITFAISIMLFVVIRWNPYKDIRELFDKK"
     gene            1690..3792
                     /gene="topA"
                     /locus_tag="PK348_03675"
     CDS             1690..3792
                     /gene="topA"
                     /locus_tag="PK348_03675"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HXK65347.1"
                     /translation="MADHKKTLVIVESPTKAKTIHKYLGKDYIIASSMGHIIDLPKSR
                     LAVDVNNDFKPEYIPIRGKAKLINDLKKKASEANNVLLATDPDREGEAISWHLSNVLK
                     EKNPDIKRIEFHEITEDAIKEAVEKARDINMDLVNAQQARRILDRLVGYYLSPILWKK
                     VKKGLSAGRVQSVALRVICEREKEIENFIPQEYWTLDALFEHHKQEFVASLVSENGNK
                     IELKTKNDVDRVLKLIEGKPFTVSNVEQKERRRKPQPPYITSKLQQDAARRLGFSASK
                     TMMVAQQLYEGVTISGEGQVGLITYMRTDSTRVAPAALASVREYLGKNFSKEYIPESP
                     NFYPNKKGAQDAHEAIRPTNVFRTPESIKNDLTPDQYKLYSLIWKRFVSSQMTPEVSL
                     VVTVTMNVEHFEFRATGSRVLFNGFTVIEKEESAEKKLLPPLDVGQVLIPKSFDPQQH
                     FTSPPPRYNEASLVKFLEESGIGRPSTYAPTINTLLKRYYVTKRGKQLVPTVLGRLVN
                     DIIVSYFESLINIDFTAKMEEKLDLIEEAHNDWVSMIKEFYEPFKLTVHHAEEHIEDM
                     KNILDEETDLVCEKCGSKMVKRLGKYGFFLACSAFPKCSNAKPFPLGKCPKCGGDVIK
                     RTSKRRGSFYACNRYPECDFATREQASEHACPKCGSLLFIRKIKKKGEMLVCLNEQCG
                     YSVTLLDEDKTDAEAHVS"
     gene            3798..>4457
                     /locus_tag="PK348_03680"
     CDS             3798..>4457
                     /locus_tag="PK348_03680"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HXK65348.1"
                     /translation="MDHYIDKFMQYLIAEKNASPLTVESYGKDLNQFIEFLYEAYDPE
                     SVTIAALTTNDIRDFIDYCFEKGNELSTIERKVATLKSFFKYLEFHNYIDNNPARDIH
                     YSRQKKFLPTFLTFDQIQALVAFECKNFSDYRDKALLEVFYSTGARVSEIANANIDDC
                     DFISGRLKVLGKGSKERIVFMNETSVTSLQEYLNERKKKFNCLTSPLFVNNNGKRLKT
                     RG"
BASE COUNT         1526 a          713 c          913 g         1305 t
ORIGIN      
        1 caatttataa tgaatatgtt ttgccagagg aaagaaaggt tcaggaatac tatacaagct
       61 ggtattcaat gtcaccgctt gaagaagcag aaaaaataat tgagttatgt aacaaaatga
      121 atgtgaacat gatttcatac tgggatgagg attatccggc tttattaaaa gaaatttcat
      181 atcctccagc ggttctatat gtacgaggta acctgcctaa taaattatcc cttgcaattg
      241 ttggtaccag aaatgatgat actcagtccg gttctatagc tgaaaaattg gcaggtattt
      301 tagcacagta tgatattgcc atcgtaagtg ggcttgccat tggaattgat agaagagcgc
      361 atataggtgc attaaaagca ggtggatcaa caataggggt catggcaaat ggtattgata
      421 tgttgtatcc ggcaagtaac cgggatattt acactgatat tatcaattca ggcaatggat
      481 gtgtgttatc agaatatcca ccaaaggtgc gtatcaccaa atggacattt gtacgcagaa
      541 atagaattat cagcggatta tcaaaagggg ttattgtcat taaagccggt gaaaaaagtg
      601 gagcacttat tactgcccag tatgctcttg aacataacag ggaattattt gtttgtactg
      661 gacattcttt tgatgaagga tacaaagggt gctataagct gttaaaagaa ggtgctcatc
      721 cagtatttaa tgaacaggat atcctggaaa ttcttagtgt tgataaatta tacaggcaac
      781 caacattatt tgaaggtatg gagtttaata aagttacttc aaatacacat tttgctgcgt
      841 atgaggatga tgatattgtc aaaacactaa aacaaggttc aatggatatt gattcactta
      901 tacgggcagt gggaaaaaac agtggcgaag ttcttgaaaa attaaccact atggaattag
      961 agggcgttat cattcgtaat gggaacctca tatcgttgaa taaataaagg tttttatgat
     1021 aattaaacga gttctaataa ttatgttagc attactgtgg gtaatcagct ttattcagat
     1081 tttttctgtg ccaccatata ttggcgatat atttgagata tataaatcgg gctattttaa
     1141 agaacttgaa agaaatatgt atataataac cgatagtttt ttacacatta aaacaatgac
     1201 aaaaccagaa tatgcctgga tatacctgga tgatttgcaa aaacgttcag gtatcagcat
     1261 tgcggtgtat gatgcacagg gtaatttaat taaagcgccg ggtatgtccg agatagttaa
     1321 caatactgag attatgaatg tatgtaatga tattaagcca caagcgcaat ttacagtatc
     1381 aggaaggctt taccatggca ttataccggt ttaccggaaa tcagaatgta atttttgtca
     1441 tcagccttcg caaaaaccat tattaggtgt aataacctat gcaattcctt ttgatggata
     1501 tatttactat acttctgaac gaataatact ttttaccatc attacgtttg ccataagtat
     1561 tatgctattt gttgttatca gatggaatcc atataaggat attagggaac tgtttgacaa
     1621 aaaataatat acattgaatt cacagtatca ataactatcg ctaaaaaatt agaaagtaag
     1681 gaagtaagta tggctgatca taagaaaacc cttgtgattg ttgaatcacc tacaaaagca
     1741 aaaacaatac ataaatattt aggaaaagat tatatcatag cttcgtcaat ggggcatatc
     1801 attgatttgc ccaaatcacg tttggcagtt gatgtgaata atgatttcaa acctgaatat
     1861 atccctatac gtggtaaggc aaagttaata aatgacctaa aaaagaaagc aagtgaagca
     1921 aacaatgttt tacttgcaac tgaccctgac cgtgaaggtg aagccatatc atggcatcta
     1981 tccaatgttt tgaaagaaaa aaatccagat ataaagcgta ttgaattcca tgagataacc
     2041 gaagatgcaa ttaaagaagc tgttgaaaaa gcccgcgaca ttaatatgga tctggtcaat
     2101 gcacagcaag cacgtcgtat tttagacagg cttgtgggtt actatttaag cccgatatta
     2161 tggaaaaagg ttaagaaggg attatccgca ggaagagttc agtctgttgc gttacgggtt
     2221 atatgtgaac gtgaaaaaga aattgaaaat tttattcctc aggaatactg gacgcttgac
     2281 gcgttatttg aacatcataa gcaggaattt gtggcatcac ttgtttcaga aaatggaaat
     2341 aaaattgaac taaaaacaaa gaacgatgtt gacagggtat taaagcttat agaaggcaaa
     2401 ccgtttaccg taagcaatgt tgaacaaaaa gaacggagaa gaaagcctca gcctccctac
     2461 attaccagca aattgcagca ggatgctgca aggcgtttag gattttccgc aagtaaaacc
     2521 atgatggtgg cacaacaatt gtatgaaggt gttacaatat caggtgaagg gcaggttggg
     2581 ctaattacat acatgcgtac cgattcaacg cgcgtggctc ctgcagcact ggcttcagtg
     2641 agggaatatc ttggtaaaaa tttttctaag gagtatattc ctgaatcgcc aaatttttat
     2701 ccaaacaaaa aaggtgcaca ggatgcacat gaagcaatac ggccaaccaa tgtgttcagg
     2761 actcctgaat caattaaaaa cgatttaaca cctgaccagt acaagctgta ttctttgata
     2821 tggaagcgat ttgtttcttc gcagatgact ccggaagttt ctttagtagt taccgttacc
     2881 atgaatgttg aacattttga gttcagggca acaggaagca gagtattgtt taatggcttc
     2941 actgtgatag aaaaagagga aagtgctgaa aagaagttac taccaccact ggatgtgggg
     3001 caggttctta tcccaaaatc atttgacccg cagcagcatt ttacttcacc gcctccacgg
     3061 tacaatgaag cttcgctggt aaaatttctg gaagaatcag gtataggcag accttctacg
     3121 tatgccccaa ccataaatac gctgttaaaa cggtattatg tgacaaaacg tggtaaacag
     3181 ctggtgccca cggttttagg aagactggtc aatgacatta tagtctcata ttttgaatct
     3241 ttgataaata ttgattttac tgcaaagatg gaagaaaaat tagatttaat tgaagaagca
     3301 cacaatgact gggtgagtat gattaaggaa ttttacgaac ccttcaagtt aaccgttcat
     3361 cacgccgaag aacatattga agatatgaaa aacatactgg atgaagaaac tgatctggtc
     3421 tgtgaaaaat gcggtagtaa aatggttaag cggttaggga aatatggatt ctttttagca
     3481 tgttcggcgt ttcctaaatg ttctaatgca aaacctttcc cccttggtaa atgtccaaag
     3541 tgtggtggcg atgtaatcaa acgtacttca aaacgtcgcg gctcatttta tgcatgtaac
     3601 aggtatcctg aatgtgattt tgccacccgt gaacaggcat cagaacacgc atgccccaaa
     3661 tgcgggagtc tcctttttat caggaaaata aagaaaaaag gcgagatgct tgtatgtttg
     3721 aatgaacagt gtggctattc agtaacactg ttagatgaag ataaaacaga tgcggaggca
     3781 catgtaagct gatacgtatg gatcactata ttgataaatt catgcaatat cttattgctg
     3841 agaaaaatgc ttcacctctt actgttgaat catatggcaa agacctgaat caattcattg
     3901 aatttttata tgaagcatat gatcctgaat cagttactat cgccgcctta actacaaatg
     3961 atatacgtga ttttattgat tattgctttg aaaaagggaa tgaactttca acaattgaaa
     4021 gaaaggttgc aacgttaaaa tcttttttta aatatcttga atttcataac tatattgata
     4081 ataatcctgc ccgcgacatc cattactcca ggcaaaaaaa atttttgcca acatttttaa
     4141 cctttgatca gattcaggcg ctggttgcgt ttgaatgcaa aaattttagt gactatcgcg
     4201 acaaagcatt gcttgaggtg ttttattcca ctggggctcg cgtttcggaa atagcaaatg
     4261 ctaacattga tgattgtgat ttcatttcag gaagattaaa ggtgcttggt aaaggatcaa
     4321 aagaaagaat tgtatttatg aatgaaactt ctgtgacgtc attgcaagaa tatttgaatg
     4381 aacgcaaaaa aaaattcaat tgtttaacca gtcccttatt tgtaaataat aacggcaaac
     4441 gattgaaaac acgaggt
//
LOCUS       DAOSZP010000117         4450 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_16033_length_4450_cov_2.828441, whole genome shotgun sequence.
ACCESSION   DAOSZP010000117 DAOSZP010000000
VERSION     DAOSZP010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4450
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16033_length_4450_cov_2.828441"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1050)
                     /locus_tag="PK348_03685"
     CDS             complement(<1..1050)
                     /locus_tag="PK348_03685"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HXK65349.1"
                     /translation="MSISKKLILIWAIVTIVSTIALGQNQYAIQGTIYSQATKKPVDF
                     ATIAIPEIKQKFYTKADGSYSILFEKPGTYTLIISSLGFKPVTVTVAINGIMQKDIVL
                     QPIAIKGAALTITGERDIQKVSRYTLTAQDLKAVPASFGDSLNALTSLPGVIRTSGFF
                     GPLVIRGADPSFNGYFIDDIPVYNPQHFGAIHSIINNDLMSEIDLYSSSFPSPYANAI
                     GAVISINTIDTVKEFGGSADVGLISSNLLLKSPVTEYNEATNQEETRGYWIVSGRYAY
                     LSLFVPAIYKLLTGDEIESVPEYWDYQFKGKYYFTKTQSITFLFFGAYDYIKFINEST
                     PPEEVDPLLASFEFKN"
     gene            complement(1054..1683)
                     /locus_tag="PK348_03690"
     CDS             complement(1054..1683)
                     /locus_tag="PK348_03690"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HXK65350.1"
                     /translation="MPFRQTLDILLQHVSQFQQQHPYIVSFIISSCIAAFTLFFTAPI
                     YVADMPEVADSLEFINIETVTAQSKRIATKEISTTTGEATPSSTDRAVGLSNESEVFD
                     ISFYPNIVPPRPIGALKKIYPQSAREKNVEANVNVEIVIAPDGKVSTVQILSIRLSKD
                     LPPEQYTVLSKEFSKAAYSILTQARFTPPVINGQRVAIKMELPMQFKLE"
     gene            complement(1683..2087)
                     /locus_tag="PK348_03695"
     CDS             complement(1683..2087)
                     /locus_tag="PK348_03695"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HXK65351.1"
                     /translation="MVQSPFLKKLRSFSLGASAADMAMLLLVFFMATTSTEPPKGVTV
                     ELPTAVTQGAEQDSIYISISKEGALYFDGKDSSIDDIKDQLAIRQNEKDRIVSITADK
                     DLPYSVVANVINILRQYDFLNVVFISQPREEQ"
     gene            complement(2090..2512)
                     /locus_tag="PK348_03700"
     CDS             complement(2090..2512)
                     /locus_tag="PK348_03700"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HXK65352.1"
                     /translation="MYWFSRKNKPQQKQSRLIEFDTTPIADLSFNLLIFFIVTASFII
                     RQGVFLSLPSLSAGTVKVEPSQVIEIYPQDNGFIADGKALDRKALLQYLTVRKSQTKE
                     AVSIIYMKPTIKYERLVDTLSAIRESGLSRISLKYNER"
     gene            complement(2512..3156)
                     /locus_tag="PK348_03705"
     CDS             complement(2512..3156)
                     /locus_tag="PK348_03705"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HXK65353.1"
                     /translation="MNKIVTFIILFLSQPLFAQELSPNSSNLWATFKQGGILMWPILF
                     LGIVGLTIIIERALFYYKIKIWDTAHIENKLNELLNQNIYKFREEAIDDLEKNMQVYY
                     NQLEKGLVLLNAVGNLAPIIGFFGTVQGMIAAFASIAAATTVNAKVVAVGIQIALITT
                     AGGLSIAVPTLAFYHFYVHILQNVYAKASELIIHKTKQLPRYSQIESTTSLEGV"
     gene            3283..4290
                     /locus_tag="PK348_03710"
     CDS             3283..4290
                     /locus_tag="PK348_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006194906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase"
                     /protein_id="HXK65354.1"
                     /translation="MNILITGTAGFIGFYTALRFLQEGHCVIGIDNMNSYYDVSLKEA
                     RNNILKQHDNYIFYHVDIANYQELSNIFKKHSFDYVVHLAAQAGVRYSLDHPFDYVHS
                     NIVGFMNILECCRHNPVKHLVYASSSSVYGLNSCVPFSVHDNVDHPVSLYAATKKSNE
                     LFAHCYSHLYSIPSSGLRFFTVYGPWGRPDMAYFKFTKAILENKPIDVYNFGDMERDF
                     TYIDDIVEGVYRITFKVPQGQPEFDTMHPDPASSSAPFRIYNIGNNKPVQLMYFIEVL
                     EKETGKKANINFLPMQPGDVLATFADIDELTDAVGYRPVTSIEEGLHKFVQWYKEYYK
                     V"
BASE COUNT         1333 a          748 c          870 g         1499 t
ORIGIN      
        1 atttttaaat tcaaatgatg caagcagtgg gtccacttct tctggagggg ttgattcatt
       61 aatgaatttt atataatcgt atgctccaaa aaacagaaag gtaatggatt gagtttttgt
      121 aaaataatat ttccctttga attgataatc ccagtattca ggtactgatt caatttcatc
      181 gccggtaagc aatttgtata ttgcaggtac aaataatgat agatatgcat aacgtccaga
      241 tactatccag tagcctctgg tttcttcctg gttagttgct tcattgtatt ctgttactgg
      301 tgatttgagt agtaggtttg atgatattaa tccaacatca gcagaaccgc caaactcttt
      361 gactgtgtca atagtattaa ttgatatgac agcaccaatg gcattggcat acggagatgg
      421 aaaacttgaa gaatacaggt caatttcact catcaggtca ttattgatga tagaatgaat
      481 tgctccaaaa tgttgtggat tataaacggg tatatcatca atgaaataac cattaaagct
      541 gggatcagca ccacggataa caagaggtcc aaaaaaaccg ctggtgcgta taacgcctgg
      601 caatgatgtt aatgcattta aagaatcgcc aaatgatgca ggtactgctt ttaaatcctg
      661 tgcggttaat gtataacgcg atactttttg tatatcacgc tcgccagtaa tagttaaggc
      721 agcacctttg attgctattg gttgtagcac aatatctttt tgcattatgc cattaattgc
      781 aacagtaacc gtcacgggtt taaaccccaa tgatgagatt ataagcgtat atgtgcctgg
      841 tttttcaaac agtatgctgt atgagccatc tgcttttgta taaaacttct gtttaatttc
      901 aggtatggct atagttgcaa aatcaaccgg ctttttagtg gcctgtgaat agattgttcc
      961 ctgtatggca tattgatttt gtcccagggc aatggtgctt acaatagtaa ctatcgccca
     1021 tattagtatt aatttcttac ttatactcat accttattcc agtttaaatt gcattggtaa
     1081 ttccattttt attgctacac gctgaccgtt aataacgggt ggtgtaaatc ttgcctgggt
     1141 aagtattgaa tatgctgctt ttgaaaattc tttgctcagt acagtgtatt gctctggtgg
     1201 aagatctttt gatagacgta ttgatagtat ttgaacagtg gaaactttgc catctggagc
     1261 aatgacaatt tcaacgttta catttgcttc aacatttttt tcacgagctg actggggata
     1321 aattttcttt aaagctccaa taggcctggg aggaacaatg ttgggataaa atgaaatatc
     1381 aaagacttct gattcatttg aaagaccaac ggctctatcg gttgatgatg gagtagcttc
     1441 tcctgttgtg gttgatattt cttttgtggc tatacgtttt gattgtgcgg taacggtttc
     1501 aatattgata aactccaggc tgtcagctac ttcaggcatg tcggcaacat agattggtgc
     1561 ggtgaagaaa agtgtgaatg ctgcaatgca tgatgaaata ataaatgaaa ctatataggg
     1621 atgttgctgt tggaattggg atacatgctg cagtagtata tccaatgttt gacgaaaagg
     1681 cattattgtt cttcccgtgg ttgagatata aatacaacat tgagaaagtc gtattgccgc
     1741 aatatattaa taacattagc tacaactgaa tatggtaaat ctttatccgc tgttattgaa
     1801 actatcctgt ccttttcatt ctggcgtatt gctaactggt ctttgatatc gtcaatggat
     1861 gaatctttac catcaaaata tagagcgcct tcttttgata tagaaatata aatactgtcc
     1921 tgttctgcac cctgggtaac tgctgtggga agctcaacag taacaccttt tggtggctcg
     1981 gttgaggttg ttgccataaa aaatactaaa agcagcatgg ccatgtcagc agctgatgct
     2041 cccagagaaa acgatcgtag tttttttaag aaggggctct gtaccatatt taccgctcat
     2101 tatattttaa tgatattctg gataagccac tttcacgaat ggcactgagc gtatcaacaa
     2161 gtctttcgta tttgatagta ggtttcatat aaattattga tacagcttct tttgtttgcg
     2221 atttgcgtac tgttaaatat tgtaataatg cttttctgtc taaggccttg ccatcggcaa
     2281 taaatccatt atcctgaggg taaatttcaa taacctgcga tggctcaact ttaactgtcc
     2341 cagcacttag cgaggggagg ctcaaaaaaa ctccctggcg tataataaat gaagcggtga
     2401 caataaaaaa tattaaaagg ttaaacgata aatctgcaat tggcgttgtg tcaaactcaa
     2461 tgagccgtga ttgtttttgc tgtggtttgt ttttacgcga aaaccagtac attatacacc
     2521 ttccagtgat gtagtacttt caatctgtga gtatcgtggt aattgttttg ttttgtggat
     2581 aataagttca gatgcttttg catacacatt ttgtaatata tgaacataga aatggtaaaa
     2641 tgctaatgtg gggacagcta tggataatcc acctgcagtt gtaataagag caatctgtat
     2701 accaacggct actacttttg cattaacggt tgttgctgct gcaattgagg caaaagctgc
     2761 aatcatcccc tggacggtac caaaaaatcc aatgattggt gctaaattac ccacagcatt
     2821 aagcagaacc agcccttttt ccagctgatt ataataaacc tgcatatttt tttcaaggtc
     2881 atctattgct tcctcacgga acttgtatat gttctggtta agtaattcat tcaacttatt
     2941 ttcaatatgt gctgtatccc atattttaat tttgtaataa aataaagcac gttcaataat
     3001 tatggttaaa cccacaatac ctaaaaacag tataggccac attaaaatgc caccctgctt
     3061 aaaggtagcc cataaattgg atgaattggg cgatagttcc tgggcaaata atggttgact
     3121 tagaaaaaga ataatgaacg ttactatttt attcatagat gacctcattt aaaaaaattc
     3181 ttgaagctgg atggatgtta ttgaacatat tccaaattta cagttatgtc aatcacttaa
     3241 ttgagtaaaa cgttttcaat taattttcag aggtaacatt acatgaacat tcttataact
     3301 ggtactgcag ggtttattgg tttttatact gcattgcgat ttttacagga agggcattgt
     3361 gttattggta ttgataacat gaacagttat tatgatgtat cgcttaaaga agcgcgtaat
     3421 aacattttga agcaacatga taattatatc ttttatcatg tagatatagc caattaccag
     3481 gaactatcga acatatttaa aaaacattct tttgactatg ttgtgcattt agctgcgcag
     3541 gcaggtgtgc gctatagcct ggaccatccg tttgactatg ttcacagcaa tattgttgga
     3601 tttatgaata tacttgaatg ctgcaggcat aatcctgtta aacatttagt ctatgcttct
     3661 tcgagctcag tgtatgggtt aaattcctgt gtgccatttt ctgtgcatga caatgttgac
     3721 catcctgttt cactgtatgc tgcaactaaa aagtcaaatg aactttttgc tcattgctac
     3781 tcacatttat attcaattcc ttcaagtggg ttacgttttt ttactgtata tggtccatgg
     3841 ggcaggcctg atatggctta tttcaaattt acaaaagcaa ttttagagaa taaacctatt
     3901 gatgtgtaca actttggaga tatggagcgc gattttacct atattgatga tatagtggaa
     3961 ggtgtatacc gaataacctt taaagtgcca caaggtcaac ctgaatttga taccatgcat
     4021 cctgatccag cttcaagtag cgcacctttc aggatttaca atattggcaa taataaacca
     4081 gtgcagttaa tgtattttat tgaggttctt gaaaaggaaa caggcaaaaa agctaacata
     4141 aactttttgc ccatgcagcc aggtgatgtg cttgctacat ttgcggatat tgatgaactt
     4201 actgatgcgg tgggttatag accagtgact tcaatagaag aaggtttgca taaatttgtt
     4261 caatggtaca aagagtatta caaagtttaa ttattactaa gaatgtttgt gacacttcaa
     4321 ttgcaggaag tgtattacca tttaattgtc tttcaatgat tgcaggagtg tttcaatagt
     4381 agttttatat gcaccctctg tatcatattg catggatttc tgcaaatagg tttttgccat
     4441 tactgtatca
//
LOCUS       DAOSZP010000118         4403 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_16248_length_4403_cov_2.775299, whole genome shotgun sequence.
ACCESSION   DAOSZP010000118 DAOSZP010000000
VERSION     DAOSZP010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4403
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16248_length_4403_cov_2.775299"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(226..1023)
                     /locus_tag="PK348_03715"
     CDS             complement(226..1023)
                     /locus_tag="PK348_03715"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HXK65355.1"
                     /translation="MKGYIKAAYIYWLLPQPVKSRCLTALDSKQQATLNKGLQHITSL
                     SIQQEISILKEAIDIINAIKIHTKHIRDISIIASCIIFGIIIILSALVSSGWHVVDSM
                     YYILQYGGLHAVLVPFIMMYAGKLMRKPFFQLVKPSHIILDILMAIIAATAIVIIFSF
                     IFPHNTVPVTKLSIIASIFAITAVPLSEELFFRGIIFLHGGKHYGYTASWFFASFVFA
                     TIHLPNTPMEFAAYFVFSSMLCFVAYRFSLFASIITHMLSNGILFIL"
     gene            1292..2380
                     /locus_tag="PK348_03720"
     CDS             1292..2380
                     /locus_tag="PK348_03720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65356.1"
                     /translation="MAEQIRTITDKGQFPKIFSLFFANNDVFLRTASGDLKIVYMGFA
                     DGTVAFKIPYVKNMPDSCVILTRKDSYIVNGVFKLKEKDADDMYVFYPVQFQIISSPR
                     KEERIELGTGKSVLFITNLISDYILQNSLALSLKKVDNVKEILRFDLEKRFPYVKIYF
                     LNEGMADPRMKYFYANPVPYFIPSMNAQPDAKGQKMFNTYVNEIYSKDYMLQNRKNLI
                     AEIAVPVLYHGKIPYGYIQVNSDKPLTDGMFSVIKRLGVVADELFKKNKLFIPETDRL
                     LIADASFSGIGIVFRERKYIRYFKEGSYVIGDILLPNGARANVLCVVRNITLMENKII
                     KVGCQIKDIDALSEVHYHEYLESAGVID"
     gene            2466..2960
                     /locus_tag="PK348_03725"
     CDS             2466..2960
                     /locus_tag="PK348_03725"
                     /inference="COORDINATES: protein motif:HMM:NF016985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HXK65357.1"
                     /translation="MNIKDLQSIILQEIDIYSTLVQLEEQKKEAITAFNGDLLFSLSK
                     DQETLILNLEKLEKSRQQFIKTHVTGHAFQPHDMTLKDFITLTRMHDNENLIVQAQKL
                     KETIEKFARLHEANKKLIHDNIEFFNVILTSLRKAVTIETGYGPEGLAKRSASGSVLM
                     NKTV"
     gene            2969..>4403
                     /gene="flgK"
                     /locus_tag="PK348_03730"
     CDS             2969..>4403
                     /gene="flgK"
                     /locus_tag="PK348_03730"
                     /inference="COORDINATES: protein motif:HMM:TIGR02492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HXK65358.1"
                     /translation="MNSTFMGIEIGKKGLLAHQQALHVTGHNISNAANEEYSRQRVVI
                     TASDPLYVPALNRANQPGNIGQGSEVAMIQRIRDEFIDDRIMVEKQAFGYWKARNDFI
                     YQIEMVYNEPSDQSIRSRLDALWEGFQELSKYPEERSTREVVKEKAIALANEVKYTYG
                     KLYELQKQANRQIGHTVEQINEYAQTIRDLNERILKAEALGDNPNDLKDKRDALIEKL
                     SQLVNINVSRTDKDELIVYIGGENLVQGEVFRPLAAIEDPDNNGMYKVLWKQTLTDVN
                     IQSGELAGLISIRDGVLRQNINDVNAFAINLTDLINEVHRDGFGKNNQTNNNFFKHIA
                     VSDNVEGNFDLNNDGINDVTALFKISGNNKVDASAAIGITGTLTFVKNNALDQEIKIN
                     YYATDTLLDVIKRVNDAKIGVVGYINHNSQLAFKATIAEDTDKKNFIIRHLEDSGQLL
                     VGYAGILKESGPQGAFDYRRVDDIRKII"
BASE COUNT         1498 a          723 c          929 g         1253 t
ORIGIN      
        1 attgagtaat tttgccagat tttgtggcaa aattgtatcg aaatgagcga gtattgcgat
       61 atactgtttg caaaatttac tcaacgacct cttttgggac agcctcctaa gattaaatat
      121 caatttcaaa atgactatat aagaatagat tctggatcaa gccccaaatg acgttatatg
      181 tagattataa gtccagctca tgattatggt atagtaatca atttattata gaataaaaag
      241 tataccattt gacagcatgt gtgtaataat gcttgcaaac aaagaaaatc tgtatgcaac
      301 aaaacataac atactgctga acacaaaata tgctgcaaat tccatcggtg tgttgggcag
      361 gtgaatggtt gcaaatacaa atgaagcaaa aaaccatgaa gcagtatacc cgtagtgttt
      421 cccaccgtgc aaaaagatga tccccctgaa aaaaagttct tcagaaagcg gtactgctgt
      481 tatggcaaat atcgatgcaa tgattgaaag tttagtaacc ggtactgtat tatgaggaaa
      541 aataaaggaa aagataataa caattgcagt tgctgctatg atagccatca gtatatcaag
      601 aattatatgt gaaggtttta ccagctgaaa aaaaggtttc ctcataagct taccagcata
      661 catcataata aaaggcacta gcactgcatg aagcccgcca tactggagaa tataatacat
      721 agaatcaacg acatgccaac cggatgaaac taaagccgaa agaatgatta tgatgccaaa
      781 gataatacat gaagcaataa tgctgatatc gcgtatgtgc tttgtatgaa tcttgatggc
      841 attgattata tcaatggctt ctttcagtat gctaatttcc tgttgtattg atagtgatgt
      901 aatgtgttgt aagcctttgt ttaacgttgc ctgttgtttt gaatccagcg ctgttaaaca
      961 cctgctttta acaggttgcg gtagaagcca atagatatat gctgctttaa tataaccttt
     1021 catgcttacg caattatgga gaaattattg ttattttcaa gcaaaaatac tcatttgact
     1081 tttatgcgta aagaaggtag attgattgta cagtaacgtt gctgtatata tctaattgat
     1141 atagtataaa atggaatttc aaaaaatttt attattgcta caaaagaatg cgtaataaat
     1201 ctttgtaatt ttcactggca tcaaaaaatt aattgaaaaa acagtccagt tgtactaaaa
     1261 ttggtcaagt attgaaacta ttaaggtaat catggcagaa cagatacgca ctataacaga
     1321 taaagggcag ttcccaaaga tattttctct tttttttgcc aataatgatg tttttttaag
     1381 aactgccagt ggtgatttaa aaatagtcta tatgggtttt gctgatggca cggttgcatt
     1441 taaaatcccc tatgttaaaa atatgcctga ttcatgtgtt attttaaccc gtaaagatag
     1501 ttatatcgtt aatggtgtgt ttaagttaaa agaaaaagat gctgatgata tgtatgtctt
     1561 ttatcctgta cagtttcaga taatttcaag tccccgaaag gaagaaagaa ttgagttagg
     1621 cacaggaaaa tcagtattgt ttattactaa ccttatttct gattatatat tgcagaattc
     1681 gctggcgcta tcgctgaaga aagtagataa tgttaaggaa attttgcggt ttgatttaga
     1741 aaaacgtttc ccctatgtaa agatatattt tttgaatgag ggaatggctg atccccgaat
     1801 gaaatatttt tatgcaaatc cagtaccgta ttttattccc agtatgaatg cacagcctga
     1861 tgctaaaggt caaaagatgt tcaataccta tgtaaacgaa atctattcaa aagattacat
     1921 gcttcaaaac agaaagaatt taatagccga gattgcagtg ccggtattat atcatggcaa
     1981 gataccctat ggctacatac aggtcaatag cgataaacca ttgactgatg gcatgttttc
     2041 ggtgataaag cggttagggg tggttgcaga tgaactgttt aaaaagaaca agctttttat
     2101 ccctgaaaca gaccgccttc ttattgctga tgcatcattt agcggaattg gcattgtgtt
     2161 cagggaacga aagtatatac gatattttaa agaaggaagc tatgttattg gggatattct
     2221 gcttcccaat ggtgccaggg ccaatgtgtt gtgtgtggtt cgcaatataa cattaatgga
     2281 aaataagata attaaagttg gttgccagat taaagacata gatgcattga gcgaagtcca
     2341 ttaccacgag tatttagaat ccgctggcgt aatagattaa gtaacctgcc aaataaaaat
     2401 cttcatgaac taacaaaaaa ttccgatagg aaacagtagt atccttatat ccaaggaggg
     2461 agttcatgaa tattaaagac ctgcaatcta tcattttaca ggagatagat atatatagca
     2521 cgttagtaca gctggaagaa caaaagaaag aagccattac agccttcaat ggtgatttgc
     2581 ttttttcatt atcaaaagac caggaaacat taatccttaa tctggaaaag ctggaaaaat
     2641 cacggcaaca gttcataaaa acccatgtaa ccggccatgc gtttcagcca cacgatatga
     2701 cgctcaagga ctttattacg ctaacccgaa tgcatgataa cgaaaatctt atagtgcagg
     2761 ctcaaaagct taaagaaaca attgagaaat ttgcacgcct tcatgaagcc aataagaagc
     2821 tgatacatga caatatagag ttttttaatg taatcctgac ttcattgcgg aaagcggtaa
     2881 caattgaaac agggtatggg ccagaaggat tggcaaaacg cagtgccagc gggtcagtat
     2941 tgatgaataa aacggtgtga ggtggtatat gaattcaaca ttcatgggaa tagaaattgg
     3001 taaaaaaggg ttacttgccc accagcaggc attgcatgtt accggtcaca atatttcaaa
     3061 tgctgcaaat gaagaatatt cgcgccagcg tgtggttata acagcatctg atccattgta
     3121 tgtgccggca ttaaacaggg ctaatcagcc tggcaatatt ggacagggca gtgaagtggc
     3181 aatgattcag cgcatccgtg atgagtttat tgatgatcgc atcatggttg aaaagcaggc
     3241 ctttgggtac tggaaagcac gcaatgattt tatctaccag attgaaatgg tgtacaatga
     3301 accatcagac cagtcaatac gcagtcggtt agatgcgttg tgggaaggat ttcaggaatt
     3361 gtcaaaatat ccggaagagc gttcaacccg tgaggtggtt aaggaaaagg cgatagctct
     3421 ggcaaatgaa gtgaaatata cgtatggtaa actgtatgaa cttcagaaac aggcaaacag
     3481 gcagattggg catactgtgg aacaaataaa cgaatatgcg cagactatcc gcgatttgaa
     3541 tgaacgaata ctgaaagccg aagcgttagg cgataatccc aatgacctca aagacaagcg
     3601 tgatgcattg atagaaaaac tgtctcagct tgtcaatatt aatgtcagcc gcaccgataa
     3661 ggatgagctt attgtgtata ttggtgggga aaacctggta cagggtgagg tgttcaggcc
     3721 acttgctgcc attgaggatc cggacaacaa tggtatgtat aaggtactgt ggaaacagac
     3781 actcactgat gttaatattc aaagcggtga gttagcaggc cttatttcaa tacgtgatgg
     3841 ggttttgcgg cagaatataa atgacgtcaa tgcattcgca ataaatctaa ctgaccttat
     3901 taatgaagtg caccgtgatg gctttgggaa aaacaatcag acaaataata atttctttaa
     3961 acacattgca gtaagcgaca atgtggaggg aaactttgac cttaacaatg atggtatcaa
     4021 tgatgtaaca gcactcttta aaatttcagg caacaataag gttgatgctt ccgctgccat
     4081 tggcattacc ggtacactta cctttgtgaa gaacaatgcg ctggatcagg aaatcaagat
     4141 aaattattat gctactgaca cgctgcttga tgtcatcaag cgtgtcaacg atgcaaagat
     4201 tggcgttgtg ggatacatca atcataacag ccagttagct ttcaaagcca ctatcgccga
     4261 agataccgat aagaaaaatt ttattatacg gcacctggaa gattcagggc aactattagt
     4321 ggggtatgca ggcattttga aagagtcagg cccccagggt gcatttgatt atcgcagggt
     4381 ggatgatata cgcaaaataa ttg
//
LOCUS       DAOSZP010000119         4402 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_16251_length_4402_cov_3.112031, whole genome shotgun sequence.
ACCESSION   DAOSZP010000119 DAOSZP010000000
VERSION     DAOSZP010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4402
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16251_length_4402_cov_3.112031"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..416
                     /locus_tag="PK348_03735"
     CDS             <1..416
                     /locus_tag="PK348_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020768525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HXK65359.1"
                     /translation="TKVGEVEGMMNLCIPYITIEPVISKLSAQYWYSSIRKGATDENA
                     AIIQSRLEAVELPIVVEVGEVEITMEELLAIQAGDVIKLPNTKINSDMILKVGGRKKY
                     KCRPGVIGNRVAVQIGEKIEEIPDELLVSKRSEEE"
     gene            complement(435..1445)
                     /locus_tag="PK348_03740"
     CDS             complement(435..1445)
                     /locus_tag="PK348_03740"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HXK65360.1"
                     /translation="MGLNPFKESNISDLQKQLQDLISERWGHIRNKLLTQWEYIKKRG
                     NERLTIMVIPHSEKKIVNLHVTLFALTGIIVSLILILTLTSIFIINHTSTIKEVSRLK
                     MYGSNSKIQIAKYKEEINKLYDIFQRLKPELSYLYSLTPGNRADTLWAKGGVSENIDV
                     EQEQPSPPIEILNIEEMVQDLETAKDVLKDIKKFMRERRKVIENTPSMWPVDGFVIAR
                     YGIRTSPYTFQKEFHEGVDIASFPGAPIRVTAPGVVESVRWDSQLGLSVSIKHKYGFV
                     TVYSHCQRVTVEEGQKVTKGEVIGYVGKTGNATRHICFYQIKIGTEFVDPLPYLNKVV
                     NQ"
     gene            2126..2977
                     /locus_tag="PK348_03745"
     CDS             2126..2977
                     /locus_tag="PK348_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002764028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HXK65361.1"
                     /translation="MIINHNLTAINSNKVLKFKHWDVDKSMEKLSSGMRINRAGDDAS
                     GLAVSEKMRTQIQGLRQAERNTEDGMSFIQTAEGYLNQTASILQRIRVLAVQSSNGIY
                     TLEDRQLIQVEVSALVDEIDRIASQAEFNKFKLLLGDFSRTNPKASMWFHMGPNMHQR
                     ERVYIGTMTSLGLNLREKTGKFLANLSTAEGANRTIGIVDEALQKISKQRADLGAYYN
                     RLEYAAKGLMNAYENIQASESRIRDADMAETMVDLTKDQILVQSGTAMLAQANLRPRT
                     ILQLLGT"
     gene            complement(3048..>4402)
                     /locus_tag="PK348_03750"
     CDS             complement(3048..>4402)
                     /locus_tag="PK348_03750"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF011443.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="putative manganese-dependent inorganic
                     diphosphatase"
                     /protein_id="HXK65362.1"
                     /translation="HIEKDRVRIIPVVSGDSYYKGVVSFAELAEFYFSATSIAQPLYY
                     FKPANFTTTIPGRCIKKGQNEEFEGHIVIGAMESAQSLNVLKNVKPEHTVLIVGNRKS
                     IIEFAIHNNFAAIIVTGTQDFNYDTATYSGWVYLSQLDTFNTLRLLTLSIPCHYIMKQ
                     SDTILPQSTVMQAREKIVNSEYRGLPVCDNGKLTGIITRSDLIKHYAKKMILVDHNEL
                     SQAVEGAETAHIIEIIDHHRLGAIKTTTPIFVYSKPVGSTCTLVYELYKYNAIQPEPH
                     IAMLLCSGILSDTAMLLSPTSTDIDKKALEELSAIAGIHYEEYGKKLLSSTESLKTID
                     PMYAVSNDCKIYQEYGISVAISQVEVITLDDIESVKESLLSALSQFAQSRNVHWALLL
                     VTDITTQNSVLFSTNFTGEQKLSFSPLGPHMYNLPGILSRKKQLLPEILRILEELSHA
                     "
BASE COUNT         1388 a          767 c          885 g         1362 t
ORIGIN      
        1 aaacaaaggt gggcgaagtt gaagggatga tgaacctgtg tataccatac attaccattg
       61 aaccggttat ttcaaagtta tctgcgcagt actggtattc aagtattcgt aaaggtgcca
      121 cagacgaaaa cgctgcaatt attcaaagcc gtcttgaagc agttgagcta cccatagttg
      181 ttgaagtagg tgaagtagaa ataaccatgg aagaattgct tgcaattcag gcaggtgatg
      241 ttattaagct gcccaatact aagattaatt cagacatgat attgaaagta ggcggaagaa
      301 agaaatataa atgtcgaccg ggggttattg gtaacagggt tgccgtgcag ataggcgaaa
      361 agatagaaga aatccctgat gaactgcttg tcagcaagcg tagtgaagaa gaataatatt
      421 taataaaata acaattattg atttacaact ttatttaagt agggaagcgg atctacaaat
      481 tcggtcccaa ttttaatctg ataaaaacat atatggcgtg tggcgttgcc tgtttttcca
      541 acatagccaa taacttcacc tttagtgacc ttttgccctt cttcaacagt aaccctctgg
      601 caatgggaat acactgtaac aaaaccatat ttatgtttta tagatacaga taatcctaac
      661 tgactatccc atcgtacgga ttcaacaaca ccaggtgcgg taactctgat aggagcacct
      721 gggaatgatg ctatatcaac accttcatga aattctttct ggaatgtata gggagatgtc
      781 cgtatcccat atcgtgcaat aacaaagcca tccacgggcc acatagatgg ggtgttttca
      841 atcactttac gccgttcacg catgaatttt ttaatatctt taagaacatc ttttgcagtt
      901 tctaaatcct gtaccatttc ctcaatattt aaaatttcaa taggcgggga gggctgttcc
      961 tgttcaacat caatattttc agacacacca ccttttgccc acagagtatc agccctgttt
     1021 ccaggtgtta atgaataaag gtacgatagt tccggtttta accgctgaaa tatatcatat
     1081 aatttattaa tttcttcttt atactttgca atctgaattt tagagtttga accatacatc
     1141 ttcaggcggg atacttcttt tattgtggat gtatggttga ttataaaaat agatgttaag
     1201 gttaagataa gtattaagga gacaataata cccgtcagtg caaaaagagt aacatgtaaa
     1261 ttaacaatct ttttctctga atgaggaata accataatgg ttaatcgctc atttcctctt
     1321 ttttttatat attcccactg agttagcagt ttattgcgaa tatgacccca tctctctgat
     1381 atgagatcct gtagttgttt ctggaggtcg cttatatttg attctttaaa tggattcaat
     1441 cccatagatt tgtttaaacc tttttttatt atgatatata tcaatatgaa ttatgtacca
     1501 tagtacatat acaagatatg tataaaaaat agcctgtcta ttgtaatagt cggccaatta
     1561 ttgtcaagtt tattcttttt tttgctcgta cactgattag cctgtttatt gaataaagaa
     1621 attacattat taacaatatg aaatacgttt tattataata atacatatta ggtttggata
     1681 ctataaatat gaaactgaaa gaaatatagc agataaatat tcacagtaaa ttttttaaaa
     1741 aaaattctaa agcaaaaatt taagattccg aaatgatgat taaggaaaaa tatgtataaa
     1801 ttttgctaaa tagataaaaa tggatgaaaa attgctatat ttcactataa atgcttataa
     1861 atggttataa atagatattt tagactttaa taatatacat attgctaccg aacatcacaa
     1921 taagctgtaa ggttctttga aaactaaaaa ttagtagctg caacgccatg tacttcattt
     1981 tttaaggtat agcatatacc agttaaaatg agggacaggg ggttgtagtg aaataaaaaa
     2041 gggtattccc ttttttaaga atcaccaacc cggttttacc cctgataggg agatcaggga
     2101 caatttaaca caaaggagtg tgtgtatgat tattaaccac aatctaacgg ccattaactc
     2161 taacaaggtc ctcaagttta aacactggga cgttgacaag tcaatggaaa agctatcatc
     2221 cggtatgcgt atcaaccgcg caggagatga tgcttcaggt ctggctgttt cagaaaaaat
     2281 gaggactcag attcagggtt tgcgtcaggc tgaacgaaat actgaagatg gcatgtcgtt
     2341 tatacagact gctgaagggt atttaaatca gactgcatca atactccagc gaatcagggt
     2401 tctggctgta cagtcttcca acggaattta tacacttgaa gacagacagc tcatccaggt
     2461 tgaagtatca gctctggttg atgagattga ccggattgca tcacaggctg aattcaacaa
     2521 gttcaaacta ttacttggtg atttctcacg aaccaatcca aaagcaagta tgtggttcca
     2581 tatgggtcca aatatgcatc agcgagaaag agtatatatt ggaaccatga cttcattggg
     2641 attgaattta agagaaaaaa ccgggaagtt tctggcaaat ctttcaactg cagagggtgc
     2701 caacagaact attggtattg tagacgaagc tttgcagaaa atatccaagc agcgtgcaga
     2761 cttaggtgct tactataaca ggttagaata tgctgctaaa ggattgatga atgcgtatga
     2821 aaacattcag gcatctgaaa gccgtatacg cgacgctgat atggctgaaa caatggtaga
     2881 tttaaccaag gatcagatcc ttgtacagag tggtactgcg atgctggctc aggcaaattt
     2941 acggccacga acaatcttac agcttttagg aacttaatat aaaaacgctg acatgtagca
     3001 gggaataccc gcccaaacaa tatacccgcc ccctgtggcg ggtattttta tgcgtgcgat
     3061 agttcttcta atattcttaa aatctcgggt agcaactgtt ttttccggga taaaatgcca
     3121 ggaaggttgt acatatgtgg cccaagaggt gaaaaactta atttttgttc cccagtaaaa
     3181 ttggtggaaa agagtacact attctgagtg gtgatatcag ttaccagtag aagtgcccag
     3241 tgaacatttc tgctttgtgc aaattgggaa agagcggaaa gtaaagattc tttgacactt
     3301 tcaatatcat caagcgtaat aacctctacc tgagatatgg ctactgatat accgtattcc
     3361 tgatatatct tacagtcatt gctgactgca tacatggggt ctatagtctt aaggctttcg
     3421 gtagatgaaa gaagtttctt cccgtactct tcatagtgta taccagctat tgctgacagc
     3481 tcttccagtg cttttttgtc aatgtcagtg cttgtaggtg ataaaagcat tgcggtatca
     3541 gataatattc cgctacaaag tagcattgct atgtgaggct caggctgtat ggcattatat
     3601 ttgtaaagtt catacaccag cgtacaggta ctgcctaccg gtttagaata tacaaatatt
     3661 ggagtggtgg tttttattgc acccaggcga tgatgatcga tgatttcaat gatatgtgca
     3721 gtttcggcac cttcaactgc ctgcgataat tcattatggt caaccagtat catttttttt
     3781 gcataatgct taatgagatc agaacgggtg atgataccgg ttagtttacc attgtcgcat
     3841 accggaagtc cccgatattc gctgtttact attttttccc gtgcctgcat cacagtgctt
     3901 tggggcaaaa tagtatctga ttgtttcata atgtaatggc agggaatgct taaggtaagc
     3961 aggcgcaatg tattgaatgt atcaagttgt gaaaggtata cccagcctga ataggtggcg
     4021 gtatcgtaat taaaatcctg tgtgccggtt acaatgatcg cagcaaaatt attgtgaatt
     4081 gcaaattcaa ttattgattt tcggttccca acaattagta cagtgtgttc tggtttaaca
     4141 ttttttaaca cattgagaga ttgggcggat tccattgcac ctatgacaat atggccttca
     4201 aattcttcgt tctgcccctt tttaatgcaa cgcccgggaa tggttgtagt aaaattagca
     4261 ggtttaaagt aatagagtgg ttgtgcaatg gatgtggcag aaaagtaaaa ttctgccagt
     4321 tctgcaaagc tgaccacccc tttatagtaa ctatcgccgc ttacaacagg tattatacgc
     4381 acacggtctt tttcaatgtg tt
//
LOCUS       DAOSZP010000120         4386 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_16332_length_4386_cov_3.310090, whole genome shotgun sequence.
ACCESSION   DAOSZP010000120 DAOSZP010000000
VERSION     DAOSZP010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4386)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4386)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4386
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16332_length_4386_cov_3.310090"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1398)
                     /locus_tag="PK348_03755"
     CDS             complement(<1..>1398)
                     /locus_tag="PK348_03755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65363.1"
                     /translation="ITAIRDPLSGAITPVRDASPCFSPDGKLIAFSSTRDGEESIYIM
                     ERDGTAIRKITEGMQPRFSSDGKELIFVKKTNTGSVVFTINIDTGTVTQITSGKYLDL
                     SPCFGKTSNDIYFMRIQYDTNGDSILDSNDKGMLFAYDVQNKEEYPLTLIEEPSATPR
                     WVPYADGIIVYSIVEGQYINIAMIPHTGIIPKQKNALRQYELALKYKEDFDDIDRYQR
                     CLFATYYFFNESSDVDSQIIIARALYELSYVQQYSFVITLLSDLGKRSKPAQIYMHYR
                     TVKDREKLPYLEQVVKELSTQNEEKIAPFFMEELADQYAGLQMQAKAKVLYNEIIARY
                     QNYTRLMYVRYKAGEQLYKEFSIPQEWITVLQSSYTYLKNAITLSIIEQTKKIPSKLR
                     YEHALKSAETYNVPVIKAIMNYVAATALMELSQNDDAIALLKQGLEYTKKLDVAYFLI
                     NVTLGKLYYEKQSDEW"
     assembly_gap    1399..1498
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1499..1988)
                     /locus_tag="PK348_03760"
     CDS             complement(<1499..1988)
                     /locus_tag="PK348_03760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65364.1"
                     /translation="MKRIACALFALLITGCASTTYRDIPFDYSQVLTKEFRFGTGRSI
                     PLTFERSVNVSGSITSDGKYFIYASNKERNNFDIYLRALNDIKTVRLTIHPARDFAPD
                     VSPDGKWCVFVSTRDDPEGDIFIMKIDPEKIVTQGDQEVPSNNITAIRDPLSGAITPV
                     RDA"
     gene            complement(1985..2581)
                     /locus_tag="PK348_03765"
     CDS             complement(1985..2581)
                     /locus_tag="PK348_03765"
                     /inference="COORDINATES: protein motif:HMM:NF018701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1318 domain-containing protein"
                     /protein_id="HXK65365.1"
                     /translation="MKHIVAIALAIVAGIIYSQARCSSSSCLKGVPSCIEIHPPAIHI
                     TGEKTTLERQLIGEYREIEKDAWVTSTVMVPSKGERRAMVAVDETMLQAIKLRELNED
                     KIRKYKDMGIIGENNNGYVGIVDEKKLQSMKDSTVIRTLVNDENNARQIIFIKSSDSK
                     DARDPKAIQFGYDFAKEQHAKAKKGDWIQKEDGRWVKK"
     gene            complement(2578..>4386)
                     /locus_tag="PK348_03770"
     CDS             complement(2578..>4386)
                     /locus_tag="PK348_03770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65366.1"
                     /translation="VFGKSTWITFAGTIEHVNSKPEIQFAMLDSNIRLNEIYEYTNVL
                     LGPKPYFNGSISIFPLTIQGNIHALTVKGAIRGQKVAFATDGFAVTIPSVEIPYTVVL
                     QNSVIEASINIGLPHFIYTLDENRSRDNGISLQCNVVYNLNTSMLDLQRIVLIHRAPE
                     IAAETMRVEVSGSVILGDIVAARINMSTVRFDLPSMAYTMPAPLKQSLTSSPITKPVT
                     VRLKADVSTSAQTNSINLRALGVIPDYSIDDLTISASVTQKPQKGKIDIAYCDIASTK
                     HQASIYMKGSVITKEDVFLDVDAGVHVAPRAPISFGNYTVSGVMKIVLSLQGTMQSLV
                     AKGSLQSQQLILSNGAGKLFVGPVDMDIPVAYTMGQSFKMPFDSADVMNIQLFKQKAN
                     FSIGKIMAPHPSRNIAFEYVKNMHGHIGFKRNIMEIKNLQAEVLRGTVTLKELNFNLA
                     DLNPQHMEFRVALNANDIDIGKLDKPESVRINKDALLSLNAQVVGKNLNIAQDFDISG
                     SINIYKVGEDFANRLFKGINEERGKSKLGMGQFAVDNSLIITGFDFYVDKGLVYPTVF
                     FKKKMLGIFVTVNNEQVRYERIPVVEFFNRVREETL"
BASE COUNT         1237 a          931 c          740 g         1378 t
ORIGIN      
        1 ccactcatca ctttgttttt cataatatag ttttcctaat gtcacattaa tcaaaaagta
       61 cgccacatcc agcttttttg tgtactccag gccctgtttc agtaatgcta ttgcatcatc
      121 attctgtgaa agttccataa gggctgttgc cgccacatag ttcataattg ctttaatgac
      181 aggcacgttg tacgtttcag ctgattttag cgcatgttca taccgcagtt tagatgggat
      241 tttttttgtt tgttcaatta ttgaaagtgt aattgcattt ttaaggtatg tataagaaga
      301 ctgaagcaca gtgatccatt cttgtggaat actgaattcc ttatacagct gctcccctgc
      361 tttataccgt acatacatta accgagtata attttgatat cgtgcaataa tctcattata
      421 taatactttg gcttttgctt gcatctgtaa gccagcatac tggtcagcaa gctcttccat
      481 aaaaaatggt gcaatttttt cttcgttttg cgtcgatagt tccttaacaa cctgctcaag
      541 atatggtagc ttttctctat ctttcacggt acgatagtgc atataaattt gtgcaggttt
      601 acttcgttta cccagatcag ataatagtgt aataacaaat gaatactgtt gaacgtacga
      661 taattcatac agtgctctgg caataataat ctgagaatca acatcactgg attcattaaa
      721 aaaataatat gtggcaaaca ggcagcgctg ataacggtca atgtcgtcaa aatcttcttt
      781 atacttcagt gccagttcat actgcctcaa agcattcttc tgttttggga taatgcctgt
      841 atgcggaatc attgcaatat taatgtactg gccttcaaca atgctgtaca caattatacc
      901 atcagcatac ggcacccagc gtggtgtggc tgaaggctct tcaatcaacg tcaggggata
      961 ctcctctttg ttttgtacat cataagcaaa tagcatgcct ttatcattag aatccagtat
     1021 gctatcacca ttggtatcat actgaatacg cataaaatat atgtcattac tggttttccc
     1081 aaagcagggc gatagatcaa ggtacttgcc actggttatt tgtgtcaccg tgccagtatc
     1141 aatattaata gtaaatacta cgcttccagt attagttttt tttacaaaga tgagctcttt
     1201 gccgtcgctg gaaaaccgtg gttgcatccc ctcagtaatt ttgcgaatgg cagtcccatc
     1261 cctttccata atgtaaatgg attcttcacc atcacgtgta gaagaaaatg caattaattt
     1321 accatcaggc gaaaaacagg gagaagcatc acgcactggt gtgatagctc ctgaaagggg
     1381 atcccgtatt gcagtgatnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnaa
     1501 gcatcacgca ctggtgtgat agctcctgaa aggggatccc gtattgcagt gatattgttt
     1561 gatggtactt cctggtcacc ctgcgtgaca atcttttcag gatcaatctt catgataaag
     1621 atatcacctt caggatcatc gcgcgtggaa acaaatacac accacttgcc atcgggcgac
     1681 acatcaggtg caaaatctct ggcaggatgg attgtcaaac gtacagtttt gatgtcattc
     1741 agtgctcgca aatagatgtc aaaattattt cgttctttat tagaagcata gataaaatat
     1801 ttaccatcag atgttatact tccactcaca tttactgatc tctcaaaggt aagtggaatt
     1861 gagcggcctg tgccaaaacg aaattctttg gtcaaaacct gtgaataatc aaacggtata
     1921 tcgcggtatg ttgttgatgc acaccctgta ataagtaatg caaaaagtgc acaagctata
     1981 cgtttcattt tttcacccag cgaccatctt ctttctgtat ccagtcaccc ttttttgctt
     2041 ttgcatgctg ctccttagca aaatcatagc caaactgaat agctttaggg tctctggcat
     2101 cttttgaatc tgaacttttt ataaagataa tctgtcgtgc attgttctca tcattaacca
     2161 gtgtacgtat tactgtgcta tctttcatgg actgtaattt cttttcatct actattccca
     2221 catacccatt attattttca cctatgattc ccatatcttt atatttacgt attttatcct
     2281 cattgagctc ccgcaactta attgcctgca gcatagtttc atctacagca accattgcgc
     2341 ggcgctcccc ttttgatggc accataacgg tagatgtaac ccatgcatct ttttcaattt
     2401 ctctatattc gcctataagc tgtcgctcca gcgttgtctt ctcgccagta atatgtattg
     2461 cagggggatg tatctcaata cagcttggta ctcctttcag gcaacttgaa gaactacatc
     2521 gtgcctgtga atatattata ccggcaacga tagcaagagc tatcgccact atatgtttca
     2581 taatgtttcc tcccttaccc tgttaaaaaa ttccacaacc ggtatacgct catagcgcac
     2641 ctgttcatta ttaacagtaa caaaaattcc cagcatcttc tttttgaaaa aaactgttgg
     2701 gtagaccaga cctttatcca cataaaaatc aaaaccggta ataatgagtg aattatccac
     2761 tgcaaactgt cccatcccta attttgactt tccccgttct tcatttattc ccttaaaaag
     2821 cctgtttgca aaatcctctc caaccttata tatgttaatg gaaccagata tatcaaaatc
     2881 ctgtgcaatg ttgaggtttt tgcccacaac ctgcgcatta agtgataaca gtgcatcttt
     2941 attaatcctc actgattcag gtttatccag ctttccaata tcaatatcat ttgcattgag
     3001 cgccacccgg aattccatgt gttgcggatt aagatcagcc agattaaaat ttaattcctt
     3061 aagagtaacc gtcccacgta aaacttcagc ctgcaaattt ttaatttcca ttatattacg
     3121 tttgaaccca atatgcccat gcatattctt aacatattca aaagcaatat ttcgcgatgg
     3181 atgtggtgcc ataattttac caatggaaaa atttgccttt tgtttaaaca gctgaatatt
     3241 cataacatca gccgaatcaa aaggcatctt aaaactttgc cccattgtat atgctactgg
     3301 tatatccata tcaactgggc caacaaaaag tttaccagca ccattactga gtattaactg
     3361 ttgagattgc aaactccctt tagcaaccaa tgattgcata gtcccctgaa gacttaatac
     3421 tatcttcata acaccactta ctgtatagtt accaaaagaa atcggtgccc gtggagctac
     3481 atgtactcct gcatcaacat caagaaacac atcttctttg gtaatcactg agcctttcat
     3541 atatatactg gcttgatgct ttgtgcttgc tatatcacag tatgctatgt caatcttccc
     3601 tttttgtggc ttctgtgtaa ctgatgctga aatagtcaaa tcatcaatag aataatccgg
     3661 aattacacca agagctctaa gattgatact atttgtttgt gcagaagtac ttacatctgc
     3721 tttaagtctt actgtcacag gttttgttat ggggcttgat gtcagcgact gtttcaacgg
     3781 tgcgggcatg gtatacgcca ttgatggaag atcaaaccgt actgttgaca tatttatacg
     3841 tgctgccaca atatcaccca aaatcactga accggaaacc tcaaccctca tagtctccgc
     3901 tgctatctca ggagccctgt gtatcagaac tattctttgg agatccagca tactggtatt
     3961 aagattatat actacgttgc actgtaagct gataccatta tcacgcgacc tgttttcatc
     4021 aagtgtataa ataaaatgtg gaagcccaat attgatggat gcctcaatga cagaattttg
     4081 cagcacaacc gtatatggta tctcaactga ggggatagta acagcaaacc catcggtagc
     4141 aaatgcaacc ttttgcccgc gaattgcacc tttcacagtt aatgcatgta tattcccctg
     4201 tattgtaaga ggaaaaattg aaattgaacc gttaaagtac ggttttggcc caagaagaac
     4261 attggtatac tcataaattt cgttaagcct gatattgctg tcaagcatag caaactgtat
     4321 ctctggttta ctgtttacat gttcgatagt acctgcaaat gtaatccagg ttgatttacc
     4381 aaatac
//
LOCUS       DAOSZP010000121         4359 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_16437_length_4359_cov_3.422398, whole genome shotgun sequence.
ACCESSION   DAOSZP010000121 DAOSZP010000000
VERSION     DAOSZP010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4359
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16437_length_4359_cov_3.422398"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..455)
                     /locus_tag="PK348_03775"
     CDS             complement(<1..455)
                     /locus_tag="PK348_03775"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HXK65367.1"
                     /translation="MKKLILLICLSLIFVLSCTKEPKQDVLKIHSFFGDVTVQLQDTT
                     RVPEIGQLLSLNDVVITGKGAIVDLVYRNVGIIRINENTTVKIESLMKGNNDDVVLTV
                     DTGKTFATLGKLKKGDNFALKSKTVIAAVRGTSFRIVASEKGSSVDVVTG"
     gene            847..1323
                     /locus_tag="PK348_03780"
     CDS             847..1323
                     /locus_tag="PK348_03780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65368.1"
                     /translation="MEIETTTCLSLEHYQLIQLLSEQYAMPMRSFISALIGFAAEIDL
                     MPPTTFTCLTYRKRGTTWKRLHLMLFADEYEFFMDIRKVWKMSLARVIEFCLENILDE
                     FLRFLDSKIVEEDYYTDSYRYRNYCFEFCREENIQTGKFYWGIHPQIIQKAKNATP"
     gene            1477..2787
                     /locus_tag="PK348_03785"
     CDS             1477..2787
                     /locus_tag="PK348_03785"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HXK65369.1"
                     /translation="MNEQISINDTTIDSLGECTYPSPLKISKFIDDSQRIAIDIEAHL
                     LEQSFHTTGTIASFENAGPRKKIFFNPETTRAAIVTCGGLCPGINNVIQGIVRMLNFQ
                     YGVKTIYGIRYGFEGLVKRYGHSFIELTPAFVHDLHEKGGTVLGSSRGHQDEKEIVAT
                     LIEHGINILFIIGGDGSLRGAYDIYQEVKRQKLPIAIVGIPKTIDNDIWFVDKTFGFS
                     TAVSTAAQAIYAAHSEAIGAKNGIGIVRVMGRDAGYIAAYVTLASNEVNYCLVPEVHF
                     DLEGPYGFLTHLEKRLYAASHAVIVVSEGAGQEYFTGPKETDASGNIKYGDIGLLLKD
                     KITEYCKKRQIPVTIKYIDTSYLVRSVPASSDDAVYCIMLAQNAVHGAMAGKTGFVVG
                     SWHSYYTYIPLSLVTKKRKRIDPNGYLWSMVKQSTCQPDFSPPQ"
     gene            2896..3888
                     /locus_tag="PK348_03790"
     CDS             2896..3888
                     /locus_tag="PK348_03790"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HXK65370.1"
                     /translation="MTTNDMDQLYRIKQLYANIGKLIVSSLELDHILEGIMEEVHTYF
                     APQNWSLLRYDPNSEELYFVISHGIDFDSVKNIRLKKGEGVAGYVVETKKPVFVPDTS
                     IDTRFSDKIDRITGFSTKSVMAVPLIFRDTIYGVIELINRAQGGIFSSDDYLILQTIA
                     DFSAIAFANSLLYEKASTIAVLDPLTGLFNSKKLTDIINVWSREAAHLRREDTTNIIA
                     VLLLDIKGLNDINNIGGHREGDRILKQLSRLLKTSLRENDMIFRTGGDMFLAIINCDN
                     YFDLKSLVERIVQKANNITLPDHPELHITIHHGLAIGTRNRIQELIEEAHRQKI"
     gene            complement(3889..4347)
                     /locus_tag="PK348_03795"
     CDS             complement(3889..4347)
                     /locus_tag="PK348_03795"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018248333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HXK65371.1"
                     /translation="MLSHHDTYMDIALEEAKLAACEDEIPVGAVIIKDETVIARAHNK
                     TRQCSDPTAHAEILAIKDAAHFLHNERLTGCLLYVTKEPCAMCAGAIVHARIEKLFIG
                     TRDYRFGACGTVLTVCGSSQLNHIPEIMFGIQEEKCKKLLQDFFGKLRST"
BASE COUNT         1372 a          846 c          798 g         1343 t
ORIGIN      
        1 cccgtaacaa catcaacact ggaacctttt tcgcttgcaa ctatcctgaa tgatgtccca
       61 cgaacagcag ctatcaccgt ctttgatttt aatgcaaaat tatcaccttt tttaagcttt
      121 cccaatgtgg caaacgtttt accagtatca acggtaagga caacatcatc attattccct
      181 ttcatcagag attcaatctt taccgttgta ttttcattaa ttctgataat accgacatta
      241 cgataaacca gatcaacaat ggcacctttt ccggtaataa caacatcatt caaagacagt
      301 aactgcccaa tttccggcac acgggtagta tcctgtaact gtactgttac atcaccaaaa
      361 aatgaatgga tttttaatac atcctgtttg ggttctttgg tgcaggataa tacaaaaatc
      421 aggcttaaac atattaatag tattaatttt ttcatgataa aactccgttc aataaaatta
      481 atatatgaat attactatct atgacatggt tacatgtaac tatcgattta taaaaaattc
      541 ctctcataac tttttttcaa aatcttaaaa tacatacaac acatgatttc tttttacaag
      601 ataagctatt ccagtattaa atcaagaatt tttctatctc attattaaac catactatac
      661 tactgcataa aattaaaaaa gtgtaaagaa aaaaaataat aactcattaa aattatgtat
      721 agtacagtat ttagatgaga tagttaatag taattataat aatttttcat aaataatgct
      781 tgcgttttac aatatttttg tatagtatat tttaaaaaaa tacagagata atttcggagg
      841 aatacaatgg aaattgaaac cacaacctgt ctttctttag aacattatca gcttattcaa
      901 ttactatcgg aacaatatgc tatgcctatg cgatctttta tatcagctct gattggtttt
      961 gctgctgaaa tagatttgat gccgcctact acatttacgt gtcttaccta tagaaagcgt
     1021 ggaacaacct ggaaacgatt gcatttaatg ctttttgctg atgaatacga attttttatg
     1081 gatattcgta aggtgtggaa gatgtcgctg gcaagagtta ttgaattttg cctggaaaat
     1141 attttagatg agtttttacg atttttagat agtaaaattg tggaagaaga ttattatacc
     1201 gatagttatc ggtacaggaa ctattgtttt gaattttgca gagaagagaa tatacaaaca
     1261 gggaaatttt actggggaat acacccccaa ataatacaaa aagcaaaaaa tgcaactcca
     1321 tgaattgaaa atttgattaa tatagccata attccagctt atattacagt atcagatttt
     1381 tttcaattga ctttataaaa aacaaaaata tcctgcatca tcaaaatttt ttgatatatt
     1441 tactatataa acatataaac tatcagattg actgttatga atgaacaaat atcaatcaat
     1501 gacacaacaa ttgattcatt gggggaatgt acatatccat ccccgttaaa aatatcaaag
     1561 tttattgatg atagtcagcg tattgccatt gacattgaag cacacctgtt agagcaatcg
     1621 tttcatacca caggtactat cgcctcattt gaaaatgcag ggccacgtaa aaaaatattc
     1681 tttaatcctg aaactaccag agcagccatt gttacctgcg gtgggttgtg cccgggtata
     1741 aataacgtca ttcagggaat tgtgcgaatg ctcaattttc agtacggtgt caaaacaatt
     1801 tatggcattc ggtatggatt tgaaggcctg gttaagcgct atggacattc ttttattgag
     1861 ctaacccctg catttgtcca tgacctccat gaaaagggcg gcactgtatt aggatcttca
     1921 cgcggacatc aggatgaaaa ggagatagtt gccacgctga tagaacatgg tattaatata
     1981 ttgtttatca ttggcggtga tggctcattg cgtggcgcgt acgacatcta tcaggaggtt
     2041 aaaaggcaaa agctccctat tgcgatagtt ggcatcccaa aaactatcga taatgatata
     2101 tggtttgtag ataaaacctt tggattttcc accgcagtgt ccacggctgc acaggctatc
     2161 tatgctgcac attctgaggc cattggtgca aagaatggca ttggtattgt gcgtgttatg
     2221 gggcgcgatg ctggatacat tgcagcatac gttaccttag cgtcaaacga ggttaactac
     2281 tgtcttgtcc ctgaagtaca ttttgactta gaagggccgt atggtttttt aacgcatctg
     2341 gaaaagcgcc tgtatgctgc atctcatgca gtcattgttg tttctgaagg tgctggtcag
     2401 gagtacttca ccgggccaaa ggaaaccgat gcttccggaa atattaaata cggggatatt
     2461 ggactgcttt taaaagataa aattacggaa tactgcaaaa aacggcaaat tccggtaacc
     2521 ataaaatata ttgacacaag ctatttagtc cgaagtgtcc ctgcttcaag cgatgatgcg
     2581 gtatactgca ttatgcttgc gcagaatgct gtgcacggtg ctatggcagg caaaaccggt
     2641 tttgtggtag gaagctggca ttcatattac acctatattc ctttgtcatt ggttactaaa
     2701 aaacgaaagc gtattgaccc caatgggtac ctgtggtcaa tggtaaaaca gagcacctgt
     2761 cagcctgatt tttccccacc ccaataacga tcataaaaat acacaaccac tgatttttta
     2821 tttgcattat ttacttatat attttatttt tcacacgaaa attgcaaata attagaatac
     2881 aagcactttt gcgatatgac tactaatgat atggatcaac tgtatagaat aaagcaacta
     2941 tatgctaata taggcaagct tatcgtttcc tcccttgaac ttgaccatat tctggaaggc
     3001 atcatggagg aagttcacac ctattttgca ccccaaaatt ggagcttgct acgctatgat
     3061 cccaattctg aggaattata ctttgtcatt tcacatggta ttgactttga cagtgttaaa
     3121 aatattcgcc tcaaaaaagg tgaaggggtt gcaggatacg tagtggaaac taaaaagcca
     3181 gtctttgtac ctgacaccag cattgatacc cgcttcagcg acaaaattga cagaattacc
     3241 ggtttttcca caaaatccgt catggctgtg ccactaatat ttcgcgacac tatttacgga
     3301 gttattgaac ttatcaacag agctcaaggt ggaatatttt cttccgacga ctatctcata
     3361 ttgcaaacca tagctgattt tagtgctatt gcctttgcca attccctgct ttatgaaaag
     3421 gcatcaacca ttgcagtact tgatccgctc accggtttgt tcaactcaaa aaaacttaca
     3481 gacataatta atgtatggtc cagagaagca gctcatcttc gccgtgagga tacaacaaac
     3541 attattgcag tactgctcct tgacattaaa ggtttaaacg atatcaacaa tattggtgga
     3601 caccgtgaag gtgatcgcat tttgaagcag ctatcacggc ttttgaaaac cagcctcaga
     3661 gaaaatgaca tgatatttcg tacaggcggc gatatgtttt tggccataat aaactgcgac
     3721 aattattttg acctgaagtc gcttgtggaa cgcattgtac agaaagcaaa caatattacc
     3781 ctgcctgacc accctgaact tcatattaca atacaccacg ggcttgccat tggtacacgc
     3841 aatcgcattc aggaactgat tgaagaagct catcgtcaga agatatagct atgtgctcct
     3901 gagtttgcca aaaaaatcct gtaacagctt tttacacttt tcttcctgta taccaaacat
     3961 tatttcaggt atatggttta actgactgct accacacacc gttagcacgg tgccacatgc
     4021 cccaaaacgg taatcccggg tgccaataaa gagcttttca atgcgtgcat gtacgatagc
     4081 gccagcgcac attgcacacg gctctttggt tacatataac aagcagcccg taagacgctc
     4141 attgtgtaaa aagtgtgccg cgtctttaat ggctaatatt tctgcatgtg ccgtggggtc
     4201 agagcattgc cgcgttttgt tgtgagcacg tgctatgact gtttcatcct taataatgac
     4261 tgcacctaca ggaatttcat cttcacatgc tgccagtttt gcctcttcaa gggcaatgtc
     4321 catgtatgta tcatgatgtg atagcattac gctgtcatg
//
LOCUS       DAOSZP010000122         4358 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_16444_length_4358_cov_3.321404, whole genome shotgun sequence.
ACCESSION   DAOSZP010000122 DAOSZP010000000
VERSION     DAOSZP010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4358
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16444_length_4358_cov_3.321404"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="PK348_03800"
     CDS             <1..285
                     /locus_tag="PK348_03800"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861547.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     pyruvate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HXK65372.1"
                     /translation="AGKFLKEYVDKKILPNDPFRILDRDGVGQLIKMAYEKGRSVNPK
                     LKLGICGEHGGEPSSVEFCHMVGLDYVSCSPFRVPIARLAAAQAAIRFPK"
     gene            434..1156
                     /locus_tag="PK348_03805"
     CDS             434..1156
                     /locus_tag="PK348_03805"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXK65373.1"
                     /translation="MRLFFISAFILTIPMMLYADYNKALKLFEQGKYDDALTEIASVL
                     DVSRDFETNTPNYNLRYLAGHIHAKKGNYESALTHLKRCAEIQKDTINPLIDIAFIYI
                     DSKRYTDAMTWARKALQIKQEPVAYYILGLAYSGIGSYWQAKEYLEKAISLDPEMYYA
                     YNELGNVLMALGRITQAQTAYSAAYALAPSSAYICNNLAMSYVQAGDVKKAIEIIEKA
                     RKLAPDNPIIVENYNRIVSLRK"
     gene            1172..2161
                     /locus_tag="PK348_03810"
     CDS             1172..2161
                     /locus_tag="PK348_03810"
                     /inference="COORDINATES: protein motif:HMM:NF012647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HXK65374.1"
                     /translation="MEIQSKYLNGDIIQWLMDDAVSLFFLKKRIPALQETTIQKDSIQ
                     IVHSASLFSPNRKTFEYFDTPGIGIMWFFSSLVESGFDISDNVVYSILKEILTFQQDD
                     GGFTLNWKPFCSTACITGQLVYSLLESGYQGKEVEKGIAWILAHQRDDGGWLHCPIGS
                     VGDSLNYLLFKRTGGGIRLEHTDVPSCAVATVMCARALMKTGNQISNDTLHRAIAYMF
                     RNDAIFKETGCRCRISYNHELLGYPFYLDYDSIDAIQFSLYLGNGSETFRIRLFNQCI
                     KKQFEDGSFPLEHRRRGCMHQFLQLPVKLKSKDKLTTVRVISMLVNTGNAVIK"
     gene            complement(2286..3065)
                     /locus_tag="PK348_03815"
     CDS             complement(2286..3065)
                     /locus_tag="PK348_03815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65375.1"
                     /translation="MKWKSKTYIRILILVSVIAFFSSAYSSEFDELDKPPEGIHKDQI
                     LLTGSVSIGVPYGDLISAEEKFTKTSTYTFENEITKLIYLSHLAFGLTISGEYLPIDY
                     IGIRSKIRRSVIVQNTNFGADYQSWKKTLYSDYSVYFGPSFHVTNRKPWDISLSPMLG
                     YSFATYKPTPIARILIDGYTPPPTQQFSSITYGVDVMLSIFFSGGLVVQIGGEWIRNN
                     IIFSSIPDAVNPQTNQTFYTKKDATIDSILFCVSAGYAFYN"
     gene            complement(3062..3682)
                     /locus_tag="PK348_03820"
     CDS             complement(3062..3682)
                     /locus_tag="PK348_03820"
                     /inference="COORDINATES: protein motif:HMM:NF024436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HXK65376.1"
                     /translation="MKFLHVLGVIITVSSLFACSTIDVAYTNKQAIQKRMKRVAVFPF
                     DIKGAGWGDEFSDAISHQFFKSGKVTTIEREAIQKILQEQQLSASGLIDDKQAVKIGK
                     LLGADVIIIGRGSALQAYRNNKFEPNCIDTFTLKAINTENGEILLTVRKEPGRAWDWR
                     YRAKFCCGLTLIWNLNDILIESSQYDDIAKQVVKQILAILQEENKR"
     gene            4019..4210
                     /locus_tag="PK348_03825"
     CDS             4019..4210
                     /locus_tag="PK348_03825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65377.1"
                     /translation="MELLIYNEEKELSSKKCFIANEYRYGKYCILGRQHATISREIKQ
                     NATHRYNEYEAEEKAYPKE"
BASE COUNT         1426 a          690 c          878 g         1364 t
ORIGIN      
        1 gcaggaaaat tcctgaaaga gtatgttgat aagaaaattt taccaaatga cccatttaga
       61 attcttgaca gggatggtgt tgggcagctt attaaaatgg catatgaaaa aggacgctct
      121 gtaaatccaa agctcaaact gggtatttgt ggtgaacatg gtggtgagcc aagttcagtt
      181 gagttttgcc acatggttgg gcttgattat gtaagttgct caccgtttag agtacctatt
      241 gcccgactgg ctgctgctca ggctgcaatt cggtttccta agtaaaaagt aaaatgtagt
      301 tttttgtgat taagataagg ttcattgtac aagtgatata tttttaatac ctcacccctg
      361 attctatttg ttaattagta gacttagggg tgagggaagt atgtaatgtt tttgggggga
      421 ataaaacgtg aaaatgagac tattttttat ttctgctttt atactaacaa ttcccatgat
      481 gctatatgca gattataata aagccttgaa actgtttgag caagggaaat atgatgatgc
      541 attaaccgaa atagcatctg ttctggatgt ttcaagagat tttgagacaa atacgccaaa
      601 ttataatctt cgttatttag caggtcatat ccatgctaag aagggcaatt atgaatcagc
      661 tctaacccat ttaaagcgtt gtgcagaaat tcagaaagat accattaatc ctctcattga
      721 tattgcgttt atatatattg attctaagcg gtatactgat gcaatgacgt gggcccgcaa
      781 agcattgcag atcaagcagg aacctgttgc atattatatt ttgggattgg cctattcagg
      841 tattggttcg tactggcagg caaaagaata tttagaaaaa gctatctcac ttgatccaga
      901 gatgtactat gcatacaatg aattgggcaa tgtgctgatg gctttaggca gaattaccca
      961 ggcacagact gcatattcgg ctgcatatgc actggcacct tcttcagcat atatttgcaa
     1021 taatcttgcc atgagttatg ttcaggctgg tgatgtgaaa aaagctattg aaatcattga
     1081 aaaggcaaga aagcttgcac ctgataaccc cataattgta gagaattata atcgtattgt
     1141 cagtttaaga aaataagcaa ctataggatt ggtggaaatt caatcaaaat atttaaatgg
     1201 tgatattatc caatggttaa tggatgatgc ggtatcatta ttttttttaa agaaacgtat
     1261 tcctgcttta caagaaacta ctatacaaaa agacagcatt caaatagtac attcggccag
     1321 tttgttttca ccaaatagaa aaacctttga gtattttgat acaccaggta ttggcattat
     1381 gtggtttttt tcatcactgg tggaaagtgg atttgatatc agtgataatg tggtgtactc
     1441 aattctcaaa gaaatactta cattccaaca ggatgacgga ggatttacgt taaactggaa
     1501 acctttctgt tcaacagcct gtattactgg ccaattagta tatagcctgc ttgaaagcgg
     1561 gtatcaggga aaggaagtag agaaaggtat agcctggatt ctggctcatc agagggatga
     1621 tggtggatgg ctgcattgtc ctattgggtc ggttggtgat tcgcttaact atttgctttt
     1681 taaacgtacc gggggaggaa tccgattaga gcatactgat gtaccaagtt gtgcggttgc
     1741 aactgtcatg tgtgctagag cactgatgaa aacaggtaac cagatcagca atgatacatt
     1801 acacagagct atcgcctata tgttccgtaa tgatgccata tttaaagaaa ctggttgcag
     1861 atgcagaatt tcctataatc atgaattgtt gggatatccg ttttatttag actatgatag
     1921 tattgatgcc attcagtttt cactttactt aggtaatgga tctgaaacgt tcaggatacg
     1981 attatttaat cagtgtataa agaaacaatt tgaggatgga agcttcccat tagaacacag
     2041 acgaagagga tgtatgcatc agtttttgca attgcctgtt aaattgaaga gtaaagataa
     2101 actgactaca gtgcgtgtga tatcaatgct ggttaataca gggaatgcag taataaaata
     2161 acttgtaagt aaataattat aaatgcaaac aatggaaatg atatggatta aatcatgatt
     2221 caggcatgtt aaggtatatg aaattgaagt tttatcttct tgtgttgtat ttacaattga
     2281 agatattaat tatagaaagc atatccggct gaaacacaga agagaatgga atcaatagtg
     2341 gcatctttct tagtatagaa tgtctggttt gtttgtggat ttacagcatc ggggattgat
     2401 gaaaatatta tattgttacg tatccattcc ccaccaatct ggacaacaag ccccccactg
     2461 aaaaatatgc tgagcatgac atcaacacca taagtaatac ttgaaaactg ctgtgtaggt
     2521 ggtggtgtat acccatcaat gagtatgcgg gctataggag ttggtttata agtagcaaat
     2581 gaatagccaa gcatgggcga tagactaatg tcccatggct ttctatttgt aacatgaaaa
     2641 gatgggccaa aataaacaga ataatcgcta tacagtgttt ttttccagga ttggtagtca
     2701 gcgccaaaat ttgtattctg tactataaca ctacgccgta tttttgaccg tatgccaata
     2761 taatcaatgg ggagatattc tcctgaaatt gttaatccaa aagctaaatg agaaagataa
     2821 atgagtttag ttatttcatt ctcaaaggta taggttgagg ttttagtaaa tttttcttct
     2881 gctgagataa gatcaccgta tgggacaccg atagaaactg agccagtaag tagtatctga
     2941 tctttatgta tgccttcagg aggcttatcg agttcatcaa attctgatga ataggcagaa
     3001 gaaaaaaatg ctatgacact aaccaggatt aaaatcctga tatatgtttt tgatttccat
     3061 ttcatctttt attttcttct tgtaaaatag ccaggatttg cttaactacc tgtttggcaa
     3121 tgtcatcata ctgggaactc tctattagaa tatcattgag attccagatt aatgttaatc
     3181 cacagcaaaa ttttgctctg taacgccagt cccatgctct ccctggttct ttgcggacgg
     3241 ttagcagaat ttcaccattt tcagtattaa tagcttttaa tgtaaacgta tcaatacagt
     3301 tgggttcaaa cttgttgttc ctgtatgcct gtaaagctga gccacggcca ataatgatta
     3361 catcagcacc taacaattta ccaattttta cagcctgttt atcgtcaatt aaaccagagg
     3421 cagaaagctg ctgttcctgc aaaatttttt gtatggcctc gcgttcgatg gtagtgactt
     3481 ttccactttt aaaaaattga tgtgagatag catctgaaaa ttcatcgccc catccagccc
     3541 cttttatatc aaatggaaaa actgcaaccc gcttcattct tttttgtatt gcctgcttat
     3601 tggtatatgc tacatcgatg gttgaacagg caaataatga agatacggtg ataataacac
     3661 ctaaaacatg taagaatttc ataattttcc acccttgttt tataatataa aaaataaaat
     3721 ctttttactc tataatgatg ttgttttatt accgcttacc gtatagtata gtatagtacc
     3781 atcttataat ggattttgac aaatattatt ttttaacaaa aaattatttt ttaattttta
     3841 atttatagta catgtttgtc aaggaatgta tttttatggc atgtgctgct ttgaaaggga
     3901 agccattgtg cagattcatg gataacatgc aacacaaaat aaaaaaatgt agcctcatac
     3961 acaacatata ctttatagtg gatgttccac caacaaaaac gaaaaagcag gtatgtatgt
     4021 ggaattactc atatataatg aggagaaaga attgtcatca aaaaaatgct ttatagcaaa
     4081 tgaatatcgt tacgggaaat attgtatatt agggagacaa catgcaacaa taagcagaga
     4141 aataaagcag aatgcaacac atagatataa tgagtatgaa gcagaagaaa aagcataccc
     4201 aaaggaataa accagattca agcgttcttt taaaaaacag caaaggttga taaaagcaaa
     4261 agtaagggtc aatgatagcc gtacagtagg tacggcgtaa tatgttgcgt gcgaggacaa
     4321 aagccttaga aggcgtccgc agcacgctgg caaggatt
//
LOCUS       DAOSZP010000123         4324 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_16596_length_4324_cov_2.836496, whole genome shotgun sequence.
ACCESSION   DAOSZP010000123 DAOSZP010000000
VERSION     DAOSZP010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4324)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4324)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4324
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16596_length_4324_cov_2.836496"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="PK348_03830"
     CDS             <1..215
                     /locus_tag="PK348_03830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65378.1"
                     /translation="CGIVINNVVDEYEPLRNFIHNENIPLLATIPHDINIARSTTQDN
                     LIDYMISHYGQHIQNIYEYCTNRVIL"
     gene            212..1099
                     /locus_tag="PK348_03835"
     CDS             212..1099
                     /locus_tag="PK348_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HXK65379.1"
                     /translation="MKPSIQQLAIVSGKGGTGKTSIAASFAKLAKRRIVVDCDVDASN
                     MPLVLPHTIIQEIPFFGEKKAVISHDRCRGCGDCVIYCRFNAIMDFYVVEPVLCEGCA
                     VCAMMCPYEAIDMVEHQTGSLVISQSDGQPFLYAKMGIGEGNSGKLVSELREKAKSIA
                     EEENYNLIIIDGPPGIGCPVIASITGTTHVLIVTEPTISGIHDLERIVQLTQHFRIPS
                     SVCINKSDISEENRQLILNICNKNNIPVIGTIPYDVNVIRAQIAGKTVVEYSDGSASQ
                     EITKLWQTVQTMLQATIAK"
     gene            1131..2021
                     /locus_tag="PK348_03840"
     CDS             1131..2021
                     /locus_tag="PK348_03840"
                     /inference="COORDINATES: protein motif:HMM:NF022077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HXK65380.1"
                     /translation="MFGKGKDGSKAQAVKPGMDSKQVTEINEELKLQENLNKIKHTIL
                     VLSGKGGVGKSTVATNLAVALASRKFKTGLLDIDIHGPSVPTLLGIEKAPLHATDHNT
                     IVPVQYNDFLKVISVGLMLPDTKEAVIWRGPLKYNLIKQFLSDVEWGELDYLVIDSPP
                     GTGDEPLSVAQLVKNPTGAVIVTTPQNVSTIDVKKSITFCHKLNIPVLGVVENMSGFV
                     CPHCGKETYIFRKGGGSEIATEMDVPFLGTIPLDPLIVENSDAGRPFAQSAAVNATVS
                     QKHFEAIVTALLQSIKSKEA"
     gene            2023..2364
                     /locus_tag="PK348_03845"
     CDS             2023..2364
                     /locus_tag="PK348_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006002451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HXK65381.1"
                     /translation="MRIALPLTEDNNFSMHFGHCDKFVLYDIDETNKHIIKKEEIPAP
                     PHQPGLLPRFLAEKGATCILAGGMGTRARQLFEEQNVVVITGVEGTEPDTIVNDYLQG
                     KLKTGANMCDH"
     gene            complement(2432..2725)
                     /locus_tag="PK348_03850"
     CDS             complement(2432..2725)
                     /locus_tag="PK348_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013932943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HXK65382.1"
                     /translation="MFKRNYLLFIEDILQNIIKINKYINNLGYDEFLDDDKTKDAVIK
                     NLIEIGEAANHIPENVLKQYNEVPWQQMIGLRNRLIHGYFVIDYDIVWTIIKE"
     gene            complement(2718..3014)
                     /locus_tag="PK348_03855"
     CDS             complement(2718..3014)
                     /locus_tag="PK348_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013798425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HXK65383.1"
                     /translation="MKSTHEVIAILSSNKDVLKEKYGVMNIAIFGSYSREDYNQDSDV
                     DIIVEFEKPIGYKYFELADYLESILQCKVDLFTYNAIKQKPLLWESVKEDIVHV"
     gene            3173..3445
                     /locus_tag="PK348_03860"
     CDS             3173..3445
                     /locus_tag="PK348_03860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65384.1"
                     /translation="MKRIIQVTLYLNGHCVETAAKETLQKLLDAMLQSETEDQNLHER
                     YQLLYDFLHTADFKQLRASDERLTGIVPTVCEVYRDENGKPALRII"
     gene            3475..3873
                     /locus_tag="PK348_03865"
     CDS             3475..3873
                     /locus_tag="PK348_03865"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HXK65385.1"
                     /translation="MIIVDTTLCTGCKRCERVCPNSAIQVNPVSKKAEINHNMCAECG
                     MCIEECPKNAIKFLASSTRNMYNMDNNKQQYAEDSTHTIVSNNDITFPRNIPGSGRGK
                     RYGMGKHHGKGRCQRKGLSHSYGKNRRRDY"
     gene            3885..>4324
                     /locus_tag="PK348_03870"
     CDS             3885..>4324
                     /locus_tag="PK348_03870"
                     /inference="COORDINATES: protein motif:HMM:NF018696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXK65386.1"
                     /translation="MDKVEIFNSYYDEYDQWFETHADTYKAEIEAIKMLLPRTTLSID
                     IGVGTGKFAKPLGITVGIEPSEKMAERAKSQGIIVIKAFAEDLPFYNNSFDVALMVTT
                     ICFVNDPQKAIHEAYRILNPQGCCIIAIVDKNSPLGKHYIKNKD"
BASE COUNT         1487 a          724 c          798 g         1315 t
ORIGIN      
        1 catgtggtat tgtaataaat aatgtggttg atgagtatga gccactgcgt aacttcattc
       61 acaatgaaaa catccctctt ttagcaacta taccgcatga tattaacatt gccaggtcca
      121 caacacagga caatcttatt gactatatga tttcccatta cggccagcat atacaaaata
      181 tttatgaata ctgtacaaac agagtgatcc tatgaaacca tcaatacaac aattggcgat
      241 agttagcggt aagggaggca ctggcaaaac aagtattgcg gcctcatttg ctaaactggc
      301 aaaacggcgc attgtggttg attgtgatgt tgatgcttca aacatgcctc ttgtgcttcc
      361 tcacaccatt atacaggaga ttccattttt tggcgaaaag aaagccgtta tatcccatga
      421 ccgatgcaga ggatgcggtg attgtgtcat ttattgcaga tttaacgcaa tcatggattt
      481 ttatgtggta gaacctgtat tatgtgaagg ttgtgcggtg tgtgcaatga tgtgtcccta
      541 tgaagcaatt gatatggttg agcatcaaac aggttcattg gtgatttcac aatctgacgg
      601 tcagccattt ctttatgcaa agatgggcat tggcgaaggt aattccggta aattagtttc
      661 cgaattgcgt gaaaaggcaa aatcaattgc agaagaagaa aactacaacc ttatcattat
      721 tgatgggccc ccgggaattg gatgcccggt catagcatcc ataaccggta caacacatgt
      781 acttattgtt acggagccaa ccatttcagg tattcatgac ttagagcgta tagtacagct
      841 tacacagcat ttcaggattc catcttcagt gtgcattaat aaaagcgata tttccgaaga
      901 aaacaggcaa ttaattctaa atatctgcaa taaaaacaat ataccggtta taggcactat
      961 tccgtatgat gtgaatgtca tcagagcaca aattgcaggt aaaacggtgg tagaatattc
     1021 agatggttca gcatcacagg aaattacaaa gctatggcaa actgtgcaaa caatgctaca
     1081 ggctacaata gcgaaatgat acattcaata aagtttaaag gaggctatag atgtttggca
     1141 aaggaaaaga tggttctaaa gcacaggcag ttaaacctgg tatggattca aaacaggtta
     1201 ctgagatcaa tgaagaactc aaattacagg aaaatctgaa taaaataaaa catacaatac
     1261 tggttttatc aggtaaaggt ggtgttggca aaagcaccgt tgctacaaat ttagctgtag
     1321 cacttgcttc cagaaaattc aaaaccgggc ttttggatat tgatattcat ggtccaagcg
     1381 taccaactct tcttggtatt gaaaaagcac cgttacatgc aacggatcat aatacaatag
     1441 taccggttca gtataatgac tttttaaaag ttatatcagt gggacttatg ctgcctgata
     1501 caaaagaggc ggtaatatgg cgaggtcctt taaaatacaa ccttattaaa cagtttttat
     1561 ccgatgttga atggggagag cttgattatc ttgtcataga ttcaccaccg ggaaccggtg
     1621 atgaaccact ttcagttgcg cagttagtaa aaaatccaac aggtgcagtt attgttacaa
     1681 caccacagaa tgtttcaact atcgatgtaa aaaaatccat tacattttgc cacaaactta
     1741 acataccggt tttaggtgtt gtggaaaaca tgagtggatt tgtatgtcct cactgcggga
     1801 aagaaacata catattcaga aaaggtggtg gcagtgagat agctactgaa atggatgtac
     1861 catttttagg aacaatacca ttagatccac taattgttga aaacagtgat gcgggaagac
     1921 catttgccca atcagctgct gtcaatgcaa cagtttcaca aaagcatttt gaagcgatag
     1981 taaccgcact attacaatca atcaagtcaa aggaggcgta acatgcgtat tgcactgcca
     2041 ttaaccgaag acaataactt ttcaatgcat tttggccact gcgacaagtt tgtgttatat
     2101 gatattgatg aaacaaataa acacatcatt aaaaaagaag agatacctgc accaccacat
     2161 cagcccggat tgttaccacg atttctggca gaaaaaggtg caacatgtat ccttgcaggt
     2221 ggtatgggaa cacgggcacg tcagcttttt gaagagcaaa atgttgttgt tataactggc
     2281 gttgagggca ctgaacctga cacaatagta aatgattatt tacagggaaa acttaaaaca
     2341 ggtgccaata tgtgtgacca ttaatactat gtgtaaaaat atgttattgg cttcttttta
     2401 tattctctag tttttcaaat agtatgggca attattcttt tataattgtc catactatat
     2461 cataatcaat gacaaaataa ccgtgtatta atctatttcg taatccgatc atttgttgcc
     2521 agggaacttc attatattgt ttaagtacat tctcgggaat atgatttgct gcctctccaa
     2581 tttcaattaa attcttgatt acggcatctt ttgttttgtc atcatcaagg aattcatcat
     2641 aaccaagatt gttgatgtat ttgtttattt taataatgtt ctggagaata tcctcaataa
     2701 aaagcaagta attcctttta aacatgaaca atatcctctt tgacactttc ccataaaagc
     2761 ggtttttgtt ttatagcatt atatgtaaac aaatccactt tacattgtaa aatactctct
     2821 aaataatcag ctaattcaaa atatttatat ccaattggtt tttcaaattc aacaataatg
     2881 tcaacatcac tatcctgatt ataatcttct cgcgaatatg aaccaaatat agctatattc
     2941 ataactccat atttttcttt taatacatct ttattcgatg acaatattgc aataacttca
     3001 tgtgttgatt tcatcgcgaa aacctataaa aaacttattt acggtattta atatatattt
     3061 taaaatatac gtatagtata tgtaacgtca attatatttt tctattattg aaataaactt
     3121 gatttttcat cattttttta atttaaagag ttagttatta agtaacaaca taatgaaaag
     3181 aataattcaa gttacattat atcttaatgg acattgcgtt gagactgctg caaaggaaac
     3241 attgcaaaag cttcttgacg caatgttaca atctgaaact gaagatcaga atcttcatga
     3301 gcggtatcaa cttttgtacg attttttaca tactgccgat tttaagcaat tgcgagcttc
     3361 agacgaaagg ctaactggta tagttccaac agtatgtgag gtatataggg atgagaatgg
     3421 gaagcctgct ttaaggatta tataaaattt ttaatgtaat aaattgagtt ttttatgatt
     3481 attgttgata ctactctctg cacaggctgt aaacgttgtg aacgtgtatg tcctaactct
     3541 gcaatacagg ttaatcctgt aagtaaaaaa gcagagatta atcacaatat gtgtgctgaa
     3601 tgtggcatgt gtattgaaga atgtcctaaa aacgcaatta aatttttagc atcatcaaca
     3661 agaaatatgt acaatatgga taacaataaa cagcaatacg cagaggatag tacacataca
     3721 attgtttcaa ataacgatat aacatttccc aggaatattc caggatctgg cagaggaaaa
     3781 cgatatggca tgggaaaaca tcatggtaag ggtagatgtc aaagaaaagg cctttcccat
     3841 tcatatggta aaaatcgccg tagagattat taatggggaa atttatggat aaagtagaaa
     3901 tatttaactc ctactacgat gaatatgatc aatggtttga aacacatgct gatacctata
     3961 aagctgagat agaagctatt aaaatgttat tacccaggac aacactgtca attgatatag
     4021 gtgttggtac tggtaaattt gctaaaccat taggcataac agttggaatt gaaccttcag
     4081 aaaaaatggc tgaaagagca aaatcacagg gaattattgt gattaaagca ttcgcagaag
     4141 accttccctt ttacaataat tcttttgatg tagcattaat ggtaacaaca atatgctttg
     4201 ttaatgatcc gcaaaaggca attcatgaag cttatcgcat attaaatcca caaggatgct
     4261 gcataatagc aattgttgat aaaaacagtc ctttaggtaa acactatata aaaaacaaag
     4321 acag
//
LOCUS       DAOSZP010000124         4316 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_16623_length_4316_cov_5.792537, whole genome shotgun sequence.
ACCESSION   DAOSZP010000124 DAOSZP010000000
VERSION     DAOSZP010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4316
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16623_length_4316_cov_5.792537"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(195..671)
                     /locus_tag="PK348_03875"
     CDS             complement(195..671)
                     /locus_tag="PK348_03875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65387.1"
                     /translation="MNCETTTCISHEHLHRLKQYSLLYNLSVSELITLLVRFAAQNKK
                     MQFIADRKIQYRERRGSDSWKRLHIVLKPFDYEIFLDAKKLFKMSVAMMIEFCIDNFL
                     FECIENTIKKQNTDNYLYGSYHYEFTTEDGVDSYHVYWGIPLKILKKILMTQQLRQ"
     gene            complement(953..2518)
                     /locus_tag="PK348_03880"
     CDS             complement(953..2518)
                     /locus_tag="PK348_03880"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HXK65388.1"
                     /translation="MKVLLINPPYPFEEAPSPPFGLMALAAFLEAQGIEVKIEDYIVN
                     RFTPERASDVVSQFAPDVVGSTGVTMNIKKALSIVKVYKELDKDIVTVLGGPHVTFDA
                     HAILRNYPFVDVIVRGEGEATFHEFLQYLHSISAYGAIKGISYRDKDNDIVHNPIREF
                     IPDINILPFPARHLAELSKYRALGFPINMMTSRGCPFNCIFCVGSKMVGRKVRYFETH
                     RVVDEFEQLAAMKFKQINVVDDLFTSNKSRCIEVCNEIKRRGIDHPWTAFARVDTVNE
                     ELLTYMRDAGCTMLCFGIESGNQEILDKVKKKITLEKCQKAVEACRKVGISPMTSYIL
                     GLPGETEETMKKTIQFAKELSDNYGFHVLAPFPGTEVREMKDYYGIEILTDDWDLYDA
                     NRAVSVTPDVSANKINEIVDVFNGSIREYILSLGEKQNRGESLTQDEQNLIGNIRSFE
                     IARDIILERLIENHPGIANGAPIDVAHELTEYLSATLPFPKDELSREVERLIQLDCLQ
                     FKYQDNRLYPQWT"
     gene            3264..3902
                     /locus_tag="PK348_03885"
     CDS             3264..3902
                     /locus_tag="PK348_03885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65389.1"
                     /translation="MNNMTQNPFKKIFEEKYEQLLQHPEMQKIKNFLSNLLSIDDQDF
                     MFMITNELGNTVTLYASKQNNNPIQSITLKLYSNADSFIPSIVFASAYDSYSIIKSYT
                     KPSNILLPECNIPLYNEVQFGKAIFHELGGCDIDEISDVLFDVASDEEMLETIAFQMA
                     QTLTIIKICELLHDACLLLVQGDRFKKLSITKPFSFFITIDDKEYITGVTID"
BASE COUNT         1393 a          835 c          660 g         1428 t
ORIGIN      
        1 cgccatggaa ttttcataca ccatggacat ggcattatat aatcataccc cgtcgttatc
       61 ataagaggtt acgagccatt tcagatgcaa tagagaaaaa ggcaaataca acaatgccat
      121 ggttaaagca tttcgcggta accctgatga taaaggcagt taaataaata gcttaaaaaa
      181 ggggatcaac caccttactg acgtaattgt tgtgtcataa ggattttttt cagtattttc
      241 agcggtattc cccagtaaac atggtagcta tcaacgccat cttcagttgt aaattcataa
      301 tgatagcttc catatagata gttatcggta ttttgttttt ttattgtatt ttcaatacat
      361 tcaaatagaa aattatcaat acaaaattca atcatcatgg caacagacat cttaaaaagc
      421 tttttggcat ctaaaaaaat ttcataatca aatggtttaa gcacaatatg taaacgcttc
      481 cagctatcac tacctctcct ttccctgtat tgaatttttc tatctgcaat aaattgcatc
      541 tttttatttt gtgctgcaaa tcgtacgagc aatgttatca attcactaac actcagatta
      601 tacaacaaag aatattgttt taaacgatgc aggtgttcat gagaaataca tgttgttgtt
      661 tcacaattca tctgtcacct ctaaaaatag aattatttcc cattgtcatt atgtgatttt
      721 ttttggtgag cattgcaatg tttcctcact gtcacatata gcgatgttaa ctaacaatgg
      781 taatcattca ttactatatt attatttata atattattgc aaagataata aaacacattc
      841 cctatgctta tgaccataaa ggaatatcta tactaaaagt agtagatgtt cccctttttt
      901 gcagaatata catacaataa tgttatctat ttcaatggtt ctttaacaaa ctttatgtcc
      961 actggggata caatctgttg tcctgatatt taaactgtaa acaatcaagt tgtataagac
     1021 gttccacttc ccgtgataat tcatctttgg gaaaaggcaa tgttgctgaa agatattcgg
     1081 tcaattcatg ggcaacatcg attggtgcac catttgcaat cccagggtga ttttcaatca
     1141 atcgttctaa aatgatatcg cgagctatct caaaagaacg tatattgcct attaaattct
     1201 gttcatcctg cgttaatgac tcgccacgat tttgcttttc acccaacgac aatatgtatt
     1261 ccctgatact gccattaaac acatcaacaa tttcattaat tttattagcg cttacatcag
     1321 gggtaactga gactgctctg tttgcatcat acaagtccca atcatccgtt aaaatttcaa
     1381 tcccatagta atccttcatt tcacgaacct cggttccggg aaatggtgcc agaacatgga
     1441 aaccataatt atcactcaat tccttagcaa actgtattgt cttcttcatg gtttcttctg
     1501 tttcaccagg caaacccagt atatacgatg tcattgggct aattcccact tttcggcatg
     1561 cctcaacagc tttctgacac ttttccagtg taatcttttt cttcacttta tccagaattt
     1621 cctgatttcc actttcaatt ccaaaacaaa gcatggtaca gccagcatcc ctcatgtagg
     1681 ttaaaagctc ttcattaacg gtatcaaccc gggcaaatgc agtccatggg tggtcaatcc
     1741 cgcgcctttt aatctcgttg caaacctcaa tacaacggct tttattcgat gtaaaaagat
     1801 catctacaac atttatctgt ttaaatttca tcgccgctaa ctgttcaaat tcatccacaa
     1861 cacgatgtgt ttcaaaataa cgcacctttc ttccaaccat cttgctgcca acacaaaaaa
     1921 tgcagttaaa cggacatcca cgtgaggtca tcatatttat tgggaaccct aacgcccgat
     1981 atttggatag ctcagccaga tgccgtgccg ggaaagggag tatattgata tcaggtataa
     2041 attcccgtat tggattatga acaatatcat tatccttatc ccgataagaa attcccttaa
     2101 tagccccata agcgcttatt gaatgtaaat attgcaaaaa ttcgtggaag gtggcttcac
     2161 cctcacctct cacaatcaca tcgacaaatg gataattgcg aagaatggca tgcgcatcaa
     2221 aggttacatg aggtccaccc agaactgtca ctatatcctt atctaactct ttatatactt
     2281 ttactatgga aagagctttt ttgatattca tggtaacgcc tgttgaacca acaacatccg
     2341 gtgcaaactg cgacacaaca tcagaagcac gctctggtgt aaacctgttt acaatataat
     2401 cttctatttt tacttcaatg ccctgtgctt caaggaatgc ggcaagggcc attaatccaa
     2461 acggaggtga tggcgcctct tcaaagggat atgggggatt gataagcaat actttcatga
     2521 tcttcctttt atcgtttcaa tttgatattc tcattatagt ttatgctata gcatttcata
     2581 ttttatggac attaaataag caaacaaatt ttggcaatat atttttgtgc ttatcataga
     2641 aattatgaca tcagcatact tatgattata ccaaacaata aattcctggc ttttcaatta
     2701 tcagtgtatt attttttttt aaaaataaaa attgcaaagg gttgggcgat agttattgtg
     2761 tattatacat aatccgaaaa tgacaggtga aggtgataat ggaatgcact gttgttttga
     2821 tcctgcattg aagcatagtt tcctttatcg cagtgatgtt taataactca attcattaca
     2881 tcttctggaa gtcactgagt atattaaggc tatgtcaatg aaaacacaat gaaatcatta
     2941 ctgcatgaca caaagcaggc agtgaagtgc ataagcttat ctttgagcat cttactttta
     3001 catcaacctt ttataacgaa cgctaaaaaa ataaacatga attttatgtg aaattttatt
     3061 tgctataata gctactattt attaattgct tgctaaactc atttattaaa atgttaatgt
     3121 aattatgata aatttttaat aaatagcttg atggaatatc tggcaagcat gttgtgctat
     3181 caatgttatg aattaaatta taagcaatta taccacacta atgtcataat taagagttta
     3241 aaaaaaatta tggagaggtt atcatgaaca atatgacaca aaatcctttt aaaaaaatat
     3301 ttgaagaaaa atatgaacag ctcttgcaac atcccgagat gcaaaaaata aaaaattttt
     3361 taagcaatct tttatcaata gatgatcagg attttatgtt tatgattacc aatgaacttg
     3421 gcaataccgt tacattatat gcatcaaaac aaaataataa tccaattcag tctataactt
     3481 taaaactata cagcaatgca gattctttta ttccttcaat agtgtttgcc tcagcatatg
     3541 attcatatag tataatcaaa tcatacacaa aaccatcgaa tatactgcta cctgaatgta
     3601 atatcccgct gtacaacgag gtacaatttg gcaaggctat ttttcatgaa ctgggaggtt
     3661 gcgacattga tgagatatct gatgttttat ttgacgttgc ttcagatgaa gaaatgctag
     3721 aaacaatagc tttccaaatg gctcaaacgt taacaataat caaaatatgt gaactgcttc
     3781 acgatgcgtg cctgctactg gttcagggcg acagatttaa aaaactatcg ataacaaaac
     3841 ctttttcatt ttttataacc attgatgata aagaatatat taccggtgta acaattgatt
     3901 aatcagtaat cattgatagt accatgattt gtgcgatagt tcctttatat catcgtggtt
     3961 tcacaggtat ttgctcatca ataataaatc ttcctttgaa aacccaaaca gcactattaa
     4021 cctggtcccg gcgaagctgt ctaaaagttg atttttacag ccccattgtt aacaaacaca
     4081 aatacatatc cctattaaac catagtagaa tcgttattgt ttgctcctct ttacttcttt
     4141 ataaaccctg ttgaaccacc tcatacggcg gtgtgccttc attgcctccc atatatccgt
     4201 tccatggcgg gtacacaacg ggcttcctcg cctgccttaa caacgatgtc gtgtagtgtt
     4261 tactattttc atttctcatt gaaactttat gtgaacatta tgttctcaac tcattt
//
LOCUS       DAOSZP010000125         4311 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_16642_length_4311_cov_3.164944, whole genome shotgun sequence.
ACCESSION   DAOSZP010000125 DAOSZP010000000
VERSION     DAOSZP010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4311
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16642_length_4311_cov_3.164944"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..391
                     /locus_tag="PK348_03890"
     CDS             <1..391
                     /locus_tag="PK348_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002769742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytoplasmic filament protein CfpA"
                     /protein_id="HXK65390.1"
                     /translation="VILDERINFLERRFNEFTYKINPHHIQPGLVLDVDVTTIKRKQY
                     MLKGMANVLNEFLYSVSRGFQDAAFATFKRRRSTVREDIDQSFAAAEMKEDIFEAAYK
                     AQSEGEIKGSVDLSSSKSKPKRGLKEL"
     gene            769..1968
                     /locus_tag="PK348_03895"
     CDS             769..1968
                     /locus_tag="PK348_03895"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HXK65391.1"
                     /translation="MYRNARIITHNLLHSLIFRIFAVCFLVLFIFSTAIFYIEKGYTR
                     TITRHGECEVVESNINSIEDSIWWAIVTSTTVGYGDKYPISRLGRIIGILTMFFGMAL
                     VGVITGNIASTLVEKQLKEDRGLKPVKIKNHYVICGWKKDMSMVLSSIAMRKEDFAGY
                     DVVLVCMAESEQIENLKSNIIFSNIHFVYGDHIDEHVLNRANIRYAKKVLVLADELGK
                     SGQDADSRTVMSVMSIKAISKSVYVCAEIIDQKYERYLRLSGCDEVILTNNYSKNIIA
                     DVFAKSGLAHVIGELIDVSTPVSLTTDEIPVHFINKPYGELVEYFARKNKILIRLLEN
                     TGNFYDRKRLALQEAQKTPDISKLVDNLRIVKTMIPNYPVLNPDKNYKIKNFSKAVLI
                     EGTSEII"
     gene            1978..2493
                     /locus_tag="PK348_03900"
     CDS             1978..2493
                     /locus_tag="PK348_03900"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HXK65392.1"
                     /translation="MTIQIDEALIKKLQKLSLFKGLDLNVIRDLAELCSYKKIKKGRT
                     VIKEGDLGDELYIIVNGEIEILKRTLQNEPYTVVAMNADDTPLYVGELAMIDKDKRSA
                     TVVAKRDCDCLVLKRKDFVAYGDANPRVGLILTRAIALQLAQRLRRTNDDVITLFSAL
                     VEEISGEAEGL"
     gene            2518..2982
                     /locus_tag="PK348_03905"
     CDS             2518..2982
                     /locus_tag="PK348_03905"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXK65393.1"
                     /translation="MKNIIMVILLLVSLPLAAQEKRTMQQEADDKKFQQGLFFVSINE
                     HQKALNEFYEYLDIYVDGLHRAKAYRFIAAIYFDNSQYTKAVSIYKRLFEEFPLTDDG
                     MYSLFQIGICYTKMGFEDDAVKAFTQLIKEYPDSNYAKDASTMLELLKIANN"
     gene            3030..4133
                     /locus_tag="PK348_03910"
     CDS             3030..4133
                     /locus_tag="PK348_03910"
                     /inference="COORDINATES: protein motif:HMM:NF024585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HXK65394.1"
                     /translation="MMNSQSSSSIPVTFTLLKVFDSVLSPQEVDFLLSMGKDAATSDA
                     IFKKVNMPYDKACEIFSSLIAKGMIWSKVYEGQELFSVSPIMLGWFEVYLCNGSTTSE
                     KREFAHYLTRLFNSWKKYNVTPLRQLRNWINKNNVPHNVIKPASPGKKIVINEAIVPE
                     TSQVLASTSINELIARYSDEGLIAVVHCFCRQWKTMEGIACRLKMPLEACIVLGKFSE
                     HAVKYGFGRFISKEEAAKIIDETKKKGGVHIVWHDKDQIDKEEIAICNCCWDCCGVLG
                     SYNRGINSLHFKTFYCAYIMQQEKCTGCGLCVTYCPVGAITLQNNKPVIDKNVCIGCL
                     QCYHKCKKDVIAIQQEEREVFLPLLKPSRIRVP"
BASE COUNT         1480 a          627 c          885 g         1319 t
ORIGIN      
        1 agttatcctg gatgagcgca tcaacttcct tgaaagaaga ttcaatgagt ttacttacaa
       61 gattaaccca catcatatcc agccgggtct tgttctggat gttgatgtaa ctaccattaa
      121 gcgcaaacag tatatgctta aaggaatggc aaacgtattg aacgaattcc tctacagtgt
      181 atcacgagga ttccaggatg cagcatttgc aacattcaag cgaagaagat caacagttcg
      241 cgaagatatc gatcagtcat ttgctgcagc ggaaatgaag gaagatattt ttgaagctgc
      301 gtataaggca cagtctgaag gtgaaatcaa aggatctgtt gacctctcat cttcaaagag
      361 caaacctaaa agaggtttga aagagctgta aaagaaaata aatacacaat tacataaaag
      421 gggagtttat ctcccctttt tttattgaaa attatcatat caaagaaata ttaatcagta
      481 aacgcataaa cctttctaat gagttacttc attaaataca attggtaatc acttgtttcc
      541 attaattatc tcatactatt atttttgcat tatataatga acttctttac catattcaga
      601 agcgacagta tacactattt taatataatg gtggtatgga ataattcatt aagtgacata
      661 atattttagt aatatttttt tataaaccta tcgataattg atatgaacta tcgcaaaaat
      721 tttatattta aatggcagaa tatgaaatta aaaaatataa attttaacat gtatagaaat
      781 gcacgaatta ttactcataa tttattacat agtctgatat tcagaatttt tgcagtttgt
      841 tttttggtac tgtttatttt ttcaacagca attttttata ttgaaaaggg atatacacgg
      901 acaattacca ggcatgggga atgtgaagtt gtagaaagta atattaactc aattgaagat
      961 tcaatatggt gggcaatagt aacgtcaaca acagttgggt atggtgataa gtatccaatt
     1021 tcgcgattgg gaagaattat tggaatactg acaatgtttt ttggtatggc gctggttggg
     1081 gtaattactg gtaatattgc ttccacgctt gtagaaaaac aattaaaaga agacagaggg
     1141 ctaaaacctg tgaaaatcaa aaatcattat gtaatatgcg gatggaaaaa agatatgtca
     1201 atggtgcttt ccagtatagc tatgagaaaa gaggattttg cagggtatga cgtagtgctg
     1261 gtatgtatgg ctgaatctga gcagattgaa aacctgaaaa gcaatattat tttttctaat
     1321 atacattttg tatatggtga ccacattgat gaacatgtat taaacagggc aaatatcagg
     1381 tatgctaaaa aagtactggt gcttgccgat gagctgggga agtcggggca ggatgcagat
     1441 tcccgtacgg ttatgtcggt aatgtcaatc aaggcaattt caaaaagtgt ttatgtatgc
     1501 gctgaaatta ttgatcagaa atatgagcgg tatctgcgtt tgtcgggatg tgatgaggtt
     1561 atactgacaa ataattacag caaaaatatt atagctgacg tatttgctaa aagtggcctt
     1621 gcacatgtta ttggtgaact aatagatgta agtacccctg tttcattgac aaccgacgag
     1681 atccctgttc attttatcaa caaaccatac ggtgaactgg ttgaatactt tgccagaaag
     1741 aacaaaatac tgattaggct tcttgaaaac acaggcaatt tttatgatcg taaacggtta
     1801 gcattgcagg aagcacagaa aacacctgat atatcaaagc ttgtagataa tttgcgaatt
     1861 gtaaaaacca tgattcccaa ttaccctgtt ttaaaccctg ataagaatta caaaataaaa
     1921 aatttttcaa aggctgtgtt aatagaaggt acaagtgaaa ttatttgagg caaaatcatg
     1981 actatacaaa ttgatgaagc attaattaaa aaattacaga aactatcgct ttttaaaggt
     2041 ctggatttaa atgtcatcag agatttagca gaattatgca gttataaaaa gattaaaaaa
     2101 ggtaggacag taattaaaga aggtgattta ggtgatgagt tgtatatcat agtgaatggc
     2161 gagatagaaa tcctgaaaag gactttgcag aatgagccat atacggtggt tgcaatgaac
     2221 gctgatgata cgccattgta tgtgggtgag ctagccatga ttgataagga taaacgttca
     2281 gccactgtag ttgcaaaacg tgattgtgat tgcctggtgc tcaaacgcaa agattttgtt
     2341 gcctatggtg atgccaatcc acgagtggga ttaattttaa caagagctat cgccctgcaa
     2401 ctggcacaaa gactaagaag aaccaatgac gatgtcataa cgttattcag tgctcttgtt
     2461 gaagagatat caggtgaggc tgaaggacta taatagattc tttagaggga gttagcaatg
     2521 aaaaacataa taatggttat actgctactt gttagtttgc cattagccgc tcaggaaaag
     2581 cgaacaatgc aacaggaagc tgatgataaa aaatttcagc aaggtctgtt ttttgtttca
     2641 attaatgaac atcagaaagc tctgaatgaa ttttatgaat atttagatat ttatgttgac
     2701 ggattgcaca gggcaaaagc atatcgattt atagcagcta tttattttga taattcacag
     2761 tataccaaag ctgttagtat ttataaacgt ctttttgaag aatttccttt gacggatgat
     2821 ggcatgtata gccttttcca gattgggata tgttacacca aaatgggttt tgaggatgat
     2881 gctgttaaag catttactca gcttataaaa gaataccctg attcaaacta tgctaaagat
     2941 gcctcaacaa tgcttgaact tttaaagatt gctaataact aacaataatg tcaaaaaata
     3001 attaccttaa attagcacga ctggcattta tgatgaatag ccagtcgtcc tcgtcaatac
     3061 ctgtcacatt tacactgctt aaggtgtttg attcagtgtt atccccacag gaggttgatt
     3121 ttttgctttc catggggaaa gatgctgcta ccagtgatgc aatatttaaa aaagttaaca
     3181 tgccctatga caaagcatgt gagatttttt catcacttat tgccaagggt atgatatggt
     3241 caaaggtata tgaagggcag gaattgttta gtgtctcgcc aattatgctg gggtggtttg
     3301 aggtgtattt gtgtaatggc agcaccacat cagaaaaaag ggaatttgca cactatctta
     3361 ccaggctttt taattcatgg aaaaaataca atgtaactcc actcaggcaa cttaggaact
     3421 ggatcaataa aaacaatgtt cctcataacg tcattaaacc tgccagtcca ggtaaaaaga
     3481 tagttatcaa tgaggcgata gttcctgaaa caagccaagt cctagcatca acatcaataa
     3541 atgaactgat tgcccgttac agtgatgaag gattgattgc cgtagttcat tgtttttgca
     3601 ggcaatggaa aacgatggag gggatagcgt gcagattgaa aatgccactg gaagcctgta
     3661 tagtgttggg taagtttagc gagcatgcgg tgaaatatgg ctttgggcga tttatttcaa
     3721 aagaagaggc agcaaagatt atagatgaga caaagaaaaa aggtggtgtt catattgtat
     3781 ggcatgacaa agatcagatt gataaagaag agatagcgat ctgtaattgt tgctgggatt
     3841 gctgcggtgt gttgggttca tataacaggg gaataaattc attgcatttt aaaacatttt
     3901 attgtgcata tattatgcaa caggagaaat gtactggttg tggtttatgt gttacatatt
     3961 gccctgtcgg agcaatcaca ttacagaata ataaaccagt aattgataaa aatgtatgta
     4021 ttggttgttt gcagtgctat cataaatgca aaaaagatgt tattgcaata caacaggaag
     4081 aaagagaagt atttttacct cttttaaaac cttcccggat acgtgtgcca taataaataa
     4141 tacaaattta cgtttgacta ttactttata ctacattttt cttgacataa aataatggga
     4201 aaataaaatg atttataaat ttgtgctaaa atagtgttat taaattcact ttataagccc
     4261 aggtggtgga actggtagac acgcatgctt gaggggcatg tgggaatatc c
//
LOCUS       DAOSZP010000126         4288 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_16747_length_4288_cov_3.140562, whole genome shotgun sequence.
ACCESSION   DAOSZP010000126 DAOSZP010000000
VERSION     DAOSZP010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4288
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16747_length_4288_cov_3.140562"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..480
                     /locus_tag="PK348_03915"
     CDS             <1..480
                     /locus_tag="PK348_03915"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF012897.2"
                     /note="Catalyzes the conversion of citrate to isocitrate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HXK65395.1"
                     /translation="MPAGQRLKYRSNIPEYAKYVFEMVDSSFSKRALDNKEKGLATVI
                     VGGVSYGQGSSREHAAICPAYLGVKIVLAKSFERIHTANLINFGILPLVFNSESDYDS
                     IQVGDTVVLNDCIKQVESGNTITLLINNKPVECTLLATERQRKIIIAGGLLNYTTQH"
     gene            complement(477..1043)
                     /locus_tag="PK348_03920"
     CDS             complement(477..1043)
                     /locus_tag="PK348_03920"
                     /inference="COORDINATES: protein motif:HMM:NF025637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HXK65396.1"
                     /translation="MIESEVLVLNAGFVPIRITTVKEAICLLLSDKAVPVVEEDTFIR
                     SPSIAIKVPSVIAILNYHHLPRRKVVFSKLNVIYRDDMQCQYCGKRFQMKHLTVDHVI
                     PRSRWEVVTGKSLKEGFSSWHNLVCACKWCNNQKGNKLMHEIGWSLLREPFEPEYIPY
                     IIVTAEKAKKKGWLPFCGFNVKIVEMIP"
     gene            complement(1263..3413)
                     /locus_tag="PK348_03925"
     CDS             complement(1263..3413)
                     /locus_tag="PK348_03925"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HXK65397.1"
                     /translation="MKQLNIVALWILVLTTKIFALSVNVDLQDAAPIKLKFIEDKQNT
                     MSFDDVVSSDKWNNNVTETINFGFTDSAYWFTFNVNNTSKIKKTALLRISYPMLDYIE
                     LYRPTENGGYEKTIEGDSFPFHQREITDVAFVYLLEIYPGTNTFYFKIKTTSSFNFTA
                     TLFSVKGFLHHLNVEQPLIWIYYGLMIIMVVFNFFVFLSIRDFSYLLYVLFITSWIFL
                     QMCLNGYAFQYLWPKQVWWGNNSLPFFMALTITSCAIFLLRSTDVLWKSRKIIIAHMI
                     LIIIPGIIIALSSLAIQYKFAIKIATGFTICAVCILFPMQAYALIKKSRIARFFAVGF
                     LGLSIGVLLYALKTFGVLPATFVTQWSIQMGSAFVVLFLSFALADRINVMRRNIEVLY
                     EDKKISESEALQKASHLEAIVNAVNVMSNDFIMVSKELELISKTFSEVSGKQSEMTLH
                     IQESFSGLQMSLEELHNALKDQKEQGKKSQEYVVTMENAYTALINENKRFIDIIESIV
                     SRAKNAENTLSSMINNMNVLQQGSHEIEQFVAVIDEISDKINLLSLNASIEAARAGEA
                     GRGFAVVADEIGKLATATAEQSGMISKRVASIAQDIHSIVVLSQDSNTALSDIFSLIG
                     TVKDGIVSFQKVVNNQTDELQKVKEQVIQIDKLSNDILRLSEKIKDVMANTLESINII
                     SSMADGIATTNSKIKEYSKTISEKSDRLSILVKG"
     gene            3955..>4288
                     /locus_tag="PK348_03930"
     CDS             3955..>4288
                     /locus_tag="PK348_03930"
                     /inference="COORDINATES: protein motif:HMM:NF025704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4398 domain-containing protein"
                     /protein_id="HXK65398.1"
                     /translation="MKQIYIYILVSCIVFSIGCASDLPIRDMSKARYGITEAEEVKAD
                     KYAPEELEKAKQYLYDTHSLLKEDKIKDAQKKSQESQAESQKAIEKSLPLYANDMLTE
                     AKETLQQAE"
BASE COUNT         1444 a          795 c          690 g         1359 t
ORIGIN      
        1 atgcctgcag gacagcgctt gaaatatcgc tctaatattc ctgagtatgc aaaatatgtg
       61 tttgaaatgg tcgatagttc attttcaaag cgtgcactgg ataacaaaga aaagggactg
      121 gcaacagtca ttgttggtgg tgtaagctat ggacagggct caagtcgcga acatgcagct
      181 atttgccctg catatctggg agtaaagatt gtactggcaa aatcgtttga acgcattcat
      241 accgcaaacc tgattaactt tggcattttg ccactggtat ttaacagtga aagcgactac
      301 gacagcatcc aggtagggga tactgttgta ctgaatgatt gtatcaagca ggttgagagt
      361 ggaaatacta taacactact cattaataat aaacctgtag aatgtacatt gttagctact
      421 gaacgacagc gaaagataat aattgctggc ggattgctta attataccac acagcattaa
      481 gggatcattt caacaatctt gacattgaat ccacagaagg ggagccaccc cttctttttt
      541 gctttttcag cagtaacgat gatatagggg atatattcgg gttcaaatgg ctcacggagt
      601 aagctccagc ctatctcatg cataagcttg ttgccttttt gattgttgca ccatttgcat
      661 gcacacacta gattatgcca gcttgaaaat ccttctttta atgattttcc tgttaccact
      721 tcccacctgc tacgtgggat aacatggtca accgtaaggt gtttcatctg aaagcgtttg
      781 ccacaatatt gacattgcat atcatcccga taaataacat tgagttttga aaatactact
      841 ttgcggcgcg gcagatgatg gtagttaagg atagcaataa cagaagggac tttaattgct
      901 attgatgggg agcggataaa agtatcttct tcaacgacgg gcacagcctt atcagaaagc
      961 aacaggcata ttgcctcttt taccgttgtt atacggatgg gaacaaaccc tgcatttagt
     1021 accagtacct cagattcaat catggtgtac cacctgtacg cacacaagcg ttacaggtaa
     1081 taaaaaccct gcatataaaa tttgcataat aatagctgtt gtacaaggta tgtcaacaat
     1141 atttagtgaa aaaatattat tttttttcac attaaaaatg aagtaaaaag tgaatggatg
     1201 aaaatgtgta gattcccgcc gttgaagttg caggagccta catcaataat taatgcagca
     1261 cattagccct ttaccagtat ggatagtcta tcagactttt cactaatagt ttttgaatat
     1321 tcttttattt tgctgtttgt tgtggcaata ccatcagcca tagatgaaat tatatttatt
     1381 gattcaagtg tgtttgccat tacatcctta attttttccg ataaccgtaa aatatcatta
     1441 gatagtttat caatttgaat gacctgctct ttgacctttt gcaattcatc agtctgatta
     1501 ttgacaacct tttgaaacga tactatacca tctttaacag ttccaattaa cgaaaagata
     1561 tctgaaagtg ctgtattgga atcctgtgaa agaacaacaa tggaatgtat atcctgtgca
     1621 atgcttgcaa cacgcttgct gatcatccct gactgttcag cggttgctgt agccaatttc
     1681 ccaatttcat ccgccacaac ggcaaacccc ctgcctgctt cccctgctcg tgcagcttct
     1741 attgatgcgt tcaatgaaag tagattaatc ttatctgaaa tttcatctat tacagcaaca
     1801 aattgttcaa tttcatggct accctgctgg agtacgttca tattatttat catactgctg
     1861 agggtattct cagcattttt tgctcttgaa acaattgatt cgatgatgtc tataaatcgt
     1921 ttattttcat taataagtgc agtatatgca ttttccattg taaccacata ttcctgagat
     1981 ttcttccctt gctctttctg atcttttaga gcattatgca attcctctaa cgacatctgt
     2041 aaaccagaaa aagactcctg aatatgcaat gtcatttcag actgtttacc tgaaacttca
     2101 gaaaatgttt tacttatcaa ttcaagttcc ttgctaacca taatgaaatc atttgacata
     2161 acattgacag cattaactat cgcctctaaa tgcgaagctt tttgtaaagc ttccgattca
     2221 cttatttttt tatcttcata tagcacttcg atgtttctcc gcataacgtt tatcctgtca
     2281 gctaacgcaa aggacaaaaa caaaacaaca aatgcagaac ccatctgtat actccactga
     2341 gttacaaatg tggctggtag tactccaaaa gtttttaatg cgtacagcaa aacccctata
     2401 gataaaccta aaaatccaac tgcaaagaaa cgagcaatac gggatttttt tattaatgca
     2461 tacgcctgca tgggaaataa tatgcatact gcacatattg taaatcctgt tgctatctta
     2521 atagcaaatt tatactgtat tgccagactg gacagggcaa taataatgcc gggtataatt
     2581 attaatatca tatgagctat tattattttt ctgcttttcc ataaaacatc agttgatcgc
     2641 agtaaaaaaa tagcacagct tgttatagtc aatgccataa aaaaaggtaa agagttatta
     2701 ccccaccata cctgtttggg ccatagatac tggaatgcat atccgtttaa acacatttgt
     2761 aaaaaaatcc atgacgttat aaataaaaca tataataaat acgaaaaatc cctaattgat
     2821 aaaaatacaa aaaaattaaa caccaccatt ataatcataa gaccatagta tatccatata
     2881 agtggctgtt caacattaag atgatgtaaa aatcctttta cagaaaaaag ggttgcagta
     2941 aaattgaatg aacttgttgt tttaatctta aagtaaaagg tatttgtacc agggtaaatt
     3001 tccaataaat aaacaaaagc cacatcggtt atctctcttt ggtgaaatgg gaagctatcc
     3061 ccttcaatag ttttttcata cccaccattt tcagtaggtc tgtataattc aatgtaatca
     3121 agcattgggt atgatattct tagaagtgca gtttttttta tttttgaagt attattaaca
     3181 ttgaatgtga accaataggc actatcagta aatccaaagt ttattgtttc agttacatta
     3241 ttattccatt tatcagaact cacaacatca tcaaacgaca ttgtattctg tttatcttca
     3301 ataaacttaa gtttgattgg cgctgcatcc tggaggtcaa cattgacaga tagagcaaat
     3361 atttttgtgg taagtacaag aatccataat gcaacaatat tcaattgctt catagtgaca
     3421 tccctcaaaa atataatagt aaaatatatt cattccttaa accacagtat ccttaacaat
     3481 attatttatt ttgattatat aattttataa ttttaaatct tccaaataat aatcacaatc
     3541 acttttttag aatgtatcag tctcttaatc tgataataac ttgtaaaata gtataacgat
     3601 tattttatta agtactgaca tgtctgtact tatttgtcaa taaaaaaact ccccttatct
     3661 tattcatgct ccctctttta taaatacatt cactaataat ccttgacctt accccataac
     3721 catttattag aaaaaatacg gatagtgtaa tatagtcatc attactttat acacagaatg
     3781 gtcttagagg cattcttgag tatatatcat caattgtgaa atcattgtaa tatttaatga
     3841 gtaaaataaa atcaaaatgt tagtgacaaa aatatcttga aatttaacca tatattggtt
     3901 atgcatacat cataacctat aaacaaacaa tatttcaata aggagacagt cgccatgaaa
     3961 caaatctata tatacatact tgtatcctgc atagtatttt ctattggatg tgcttctgac
     4021 ctgcccatca gggatatgtc caaagcccgc tatggaataa ccgaagctga agaagtaaaa
     4081 gcagacaaat atgcacctga agagctggaa aaagctaaac aatatttata tgacacgcat
     4141 tcattgctta aagaagataa aataaaggat gctcagaaaa aatcacaaga atcacaggca
     4201 gaatcgcaaa aagcaataga aaaatccctg ccactgtatg ctaacgatat gctcacagaa
     4261 gctaaagaga ccttgcagca ggcagaaa
//
LOCUS       DAOSZP010000127         4283 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_16769_length_4283_cov_3.630558, whole genome shotgun sequence.
ACCESSION   DAOSZP010000127 DAOSZP010000000
VERSION     DAOSZP010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4283)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4283)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4283
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16769_length_4283_cov_3.630558"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..673)
                     /locus_tag="PK348_03935"
     CDS             complement(<1..673)
                     /locus_tag="PK348_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012957483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HXK65399.1"
                     /translation="MGKKFLIAIDLGTTGNRVFCFDEHGLPISSSYAEFTQHFPKPGW
                     VEHDANEIWESVVKLLPEAIQKGNLDPKDAIAIGITNQRETSVLWNEETGKPIYHAIV
                     WQCRRTTDICNQLKEAGHEQLFREKTGLVIDAYFSGTKVKWMLDNVPGARDLAKSGKL
                     LFGTIDTWILWKLTGGKSHKTDYTNASRTLMFNIKEKIWDLDILGILNIPRTILPEVR
                     ESASYF"
     gene            1013..2056
                     /locus_tag="PK348_03940"
     CDS             1013..2056
                     /locus_tag="PK348_03940"
                     /inference="COORDINATES: protein motif:HMM:NF015087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HXK65400.1"
                     /translation="MDLFEKNYLNKLQNNEPVFIAEIGLNHNGKLDEAIALIQKAAQA
                     GADLVKFQVFNAEKFYSVYTKSLLNHEKPKYDDSSIQFFKKFELSFNDYRTLQHVAYD
                     HGVVFFASCFDDDSFAMMENLNVPLYKIASSEITNVPLLQNIVATHKPVIVSTGISLQ
                     HEIAHAIHLLQKEQCNVVLLHCVSLYPVPHDAINLLRIKRLQKLYGLPVGFSDHTKDN
                     RASMMAVAMGARVFEKHFKLSHNHDCADKDVSLTPEEFTAYIEDINMALAMLGDGSID
                     YKEGEAAVARSARRSIFAARDIPAGTSLNYSDIIVKRPGVGLSPLHSDAIIGKKVKHT
                     IHKDMPIYGEDLE"
     gene            2320..2850
                     /locus_tag="PK348_03945"
     CDS             2320..2850
                     /locus_tag="PK348_03945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65401.1"
                     /translation="MKKIAIVFLFFIVVTPAYASTIDYIFNDNQRLILNSATDIIQAS
                     LGYDDIRELVYITFWSTQPADAKKIDTFNMYIAQQYKTSPDDVIFIYERLLQSVYMIE
                     YMADLAKSKKKWKFYYYYSDTLLPDTRRFCDTLKQAIIKADPSIIEHINKRDVRIKSY
                     AIDIVTYREALYGGGF"
     gene            complement(2971..3753)
                     /locus_tag="PK348_03950"
     CDS             complement(2971..3753)
                     /locus_tag="PK348_03950"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HXK65402.1"
                     /translation="MKTKKIKRIQSIERAHAILSLFINDKKPLGISDFARQLTLPKTT
                     IQGIVQTLCHLGYLEKDHETGKYRLGPYVFQLGMKYATNLDLVSIARVWAERLCFQFR
                     QPVNIGMLVGDKVVIVLRVEPENRFMSYPQAGSVIPTHTTCIGKLLCAYMEKERLEEI
                     LKDYAFQMLTPNSITSKEGFMKEIKKVRQAGISFDNQESVMGLAGIGGPIFNYSGIIA
                     AFAITGNADFILKHKQDIIHAVRDTSLQLSVQLGYNPDSKIL"
     gene            3877..>4283
                     /locus_tag="PK348_03955"
     CDS             3877..>4283
                     /locus_tag="PK348_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015906092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HXK65403.1"
                     /translation="MDILKKFEITDPYEYVANLKKATGKSVLAYMCSYTPEEIIYAAG
                     IHPFRIFGQVETISKADAHLQAYSCSLVRGALEDALQGRLSFVDGAVFPHTCDSIQRL
                     SDIWRLNAGMKHHFDVVWAVKLNTDSARKYMRDV"
BASE COUNT         1320 a          756 c          831 g         1376 t
ORIGIN      
        1 caaaatatga tgctgattcc ctcacttcag gcagtatggt gcgcggtata ttcagtattc
       61 ctaaaatatc cagatcccaa attttctctt ttatattgaa catcagtgta cgggaagcat
      121 tagtgtaatc ggttttatga gatttgccgc cagttaactt ccacaaaatc caggtgtcga
      181 tagtcccaaa taatagctta ccacttttag ccagatcacg tgcaccgggc acattatcaa
      241 gcatccattt aacttttgtg cccgaaaaat atgcatcaat cacaaggcca gtcttttcgc
      301 gaaatagctg ttcgtggcca gcttctttca gttgattgca tatatcagtt gttctacggc
      361 attgccatac tattgcgtgg taaattggct ttcctgtttc ttcattccat agcactgagg
      421 tttctcgctg gttggtgatg ccaattgcta tcgcatcttt tggatccaga ttcccttttt
      481 gtatggcttc aggtaacagc tttacaacgg attcccagat ttcattagca tcgtgttcaa
      541 cccatccagg ttttggaaaa tgctgtgtaa attcagcata gctgcttgaa attggcagtc
      601 catgttcgtc aaaacagaat acgcggttac cagtagtacc taaatcaatg gcaataagga
      661 actttttacc cataatacaa cccctccatt atttttatgt gcgatagtta ctgtacatta
      721 aagctacata atagctgaaa atagtatggt aaaatgtgtt taaggcttta cagtgaagtc
      781 tttagaattt taataccgtt gagcgcttat cttactgttt tcattaataa tttgtcaatt
      841 aaattttgag gaagaaaatg agtgatatat aaaaatatct tgcaaaggta tggcttcttt
      901 gaaacactgt gtagcgttta ttacgttttt atcatttgta aaaaagcaat gtcaggagat
      961 caggaattat gaatatagtt aagaatatga tactattgca agggaatagt atatggattt
     1021 atttgaaaaa aattatctga ataagctgca aaataacgaa ccagtattta ttgcagaaat
     1081 tgggcttaac cacaatggca aattggatga agccattgct ttaattcaaa aagcagcaca
     1141 agcaggtgct gatttagtta agtttcaagt ttttaatgca gaaaaatttt attcagtgta
     1201 tacaaaatcg cttcttaacc atgaaaaacc gaagtatgat gatagcagca tacagttttt
     1261 taaaaagttt gaactttcat ttaacgatta cagaacgctc cagcacgttg cttatgatca
     1321 tggtgttgta ttttttgcat catgttttga tgatgattct tttgcaatga tggaaaacct
     1381 taatgttcca ttgtacaaaa tagcttcgtc agagattacc aatgtgccat tgttacagaa
     1441 cattgtagcc actcataaac ctgtgattgt ttctacaggc atttcattgc aacatgagat
     1501 tgctcatgca atacaccttt tgcaaaaaga acagtgcaat gttgtgctgt tgcactgtgt
     1561 atcactgtat ccagtaccac atgatgcaat taatctgttg cgaatcaaaa ggttacaaaa
     1621 attatatggt ctaccagttg gattttcaga tcatacaaag gataatcgtg cttccatgat
     1681 ggctgttgca atgggtgcac gggtatttga aaagcatttt aaattatcgc acaaccatga
     1741 ttgtgcagat aaggatgtat ctcttacacc agaagagttt acagcctaca ttgaagatat
     1801 taatatggct ctggcaatgc ttggtgatgg atcaatcgat tataaagaag gtgaagcagc
     1861 cgttgcccgt tcggcacggc gaagcatttt tgcagcacgg gatatacctg cagggacaag
     1921 ccttaactac agtgatatca tcgtcaaacg accgggcgtg ggcctatcgc cgctacatag
     1981 tgatgctatt atagggaaaa aagttaaaca tacaatacac aaagacatgc ctatttatgg
     2041 tgaggatctt gaatgagtgt tgatcttttc catcgctttt tgcaatggct tttacaatta
     2101 atccaccaca atgcaagcgt ggcttatttc tttttaaaac tattgaagaa gcaaatgaat
     2161 ttagattaaa cgatcagatt gaaaaagtct taaaattgtt ttaggagtat actcactaaa
     2221 ctataaaata aaattgttga ataaaaagag ttataacgaa acattgtgta gtgattgttt
     2281 tctatatact ataaaactgc aaggtgaggg aatctcagaa tgaaaaagat agccattgta
     2341 tttctttttt ttattgtagt aactccagca tatgcatcaa ccattgatta tatattcaat
     2401 gataaccaac ggttaatttt gaattccgct accgacataa tccaggcttc attagggtat
     2461 gatgatatca gggagttagt gtatataaca ttctggagca ctcagcccgc tgatgccaaa
     2521 aaaattgata cattcaatat gtatattgct cagcaataca aaaccagccc tgatgatgtt
     2581 atttttattt atgagcgttt attgcagtca gtgtacatga ttgaatacat ggctgatctg
     2641 gcaaagtcga aaaagaaatg gaagttctat tactactatt cagatacact gttacctgat
     2701 accaggcggt tttgtgatac tttaaaacag gctattataa aagcagatcc atctattata
     2761 gaacatataa ataaacgcga tgtaagaata aaaagctatg caattgacat cgttacatac
     2821 agagaagcat tgtatggagg agggttttaa aaaaaagccc cagaggggct aaaagttttg
     2881 ttataggctg cattagcact cttttattat gagtgctaac aataatatac ataaaattca
     2941 ttatatcgtc aagtattttt gttatatttt ttagagtatt ttgctgtcag gattataccc
     3001 taactgcact gacaattgaa gtgaggtatc tcgtacagca tggataatgt cctgtttgtg
     3061 tttgagtata aaatcagcat taccggtaat ggcaaatgct gcaataatac cactataatt
     3121 aaagataggc ccaccaattc cagccaatcc catcactgac tcctgattat caaaactaat
     3181 tccagcctgg cgcacctttt ttatttcttt cataaagcct tcttttgatg ttattgaatt
     3241 tggtgtcagc atctggaaag cataatcttt tagaatttct tctaatcgct ctttttccat
     3301 ataggcacac agtaacttgc ctatacaggt ggtatgtgtg ggtataactg aaccggcttg
     3361 aggatatgac ataaacctgt tttcaggctc aacgcggagt actatgacta ctttatctcc
     3421 cacaagcatg ccaatattga ccggttgcct gaattgaaag catagacgtt cggcccatac
     3481 cctggcaatg ctcaccaggt caaggttggt ggcatatttc attcctaact ggaatacata
     3541 cggaccaaga cggtatttgc ctgtttcgtg atctttttcc agatatccta aatgacacag
     3601 tgtttgaact attccctgta tggtggtttt gggtaatgtt aattgtcggg caaaatcgct
     3661 tatgcccagc ggttttttat cattaatgaa cagagataaa atagcgtgtg cacgctcaat
     3721 tgattgaatg cgtttaattt tttttgtctt cataattgtc atactatata acaaaaagtt
     3781 attgacagtt tacatattca aatttatatt gaatataata tcgtacaatg tacgatatta
     3841 tcgtacattg tcaactactt tttgtaggag tatagaatgg atatactgaa aaaatttgag
     3901 attactgatc cgtatgagta tgttgctaat ttaaaaaagg caacaggcaa gagtgtgctt
     3961 gcatatatgt gttcgtacac accggaagag atcatctatg ccgcaggtat tcacccattc
     4021 agaatatttg gtcaggtgga gacaatctct aaggcggatg cacatctgca ggcatacagc
     4081 tgttcgctgg tacggggtgc actggaggat gcactgcagg gcaggctatc atttgtggat
     4141 ggtgccgtgt ttccgcacac ctgtgattca attcagcggc tttccgatat atggcgcctg
     4201 aatgctggca tgaagcatca ttttgatgtg gtatgggctg ttaagctcaa tactgatagt
     4261 gcacgaaaat atatgcgcga tgt
//
LOCUS       DAOSZP010000128         4279 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_16794_length_4279_cov_3.357481, whole genome shotgun sequence.
ACCESSION   DAOSZP010000128 DAOSZP010000000
VERSION     DAOSZP010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4279
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16794_length_4279_cov_3.357481"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="PK348_03960"
     CDS             complement(<1..190)
                     /locus_tag="PK348_03960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65404.1"
                     /translation="MKQYISILFISFAFILLSCSQQISIQGKPDVLIKKYLKDDSTYV
                     IMCKGYPKPNTTGIQATET"
     gene            complement(309..737)
                     /locus_tag="PK348_03965"
     CDS             complement(309..737)
                     /locus_tag="PK348_03965"
                     /inference="COORDINATES: protein motif:HMM:NF040320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HXK65405.1"
                     /translation="MAFQVSVFAENKPGKIERITSILAKHNINIRAITINDSGDYGII
                     KLLLDRPEDGCTALKEEGVAATLKEIVAVKIGDKPGSLYKVAAILKDHAINVDDAYGF
                     TVKPHEESVFVFQVDNPKAAEKALKDAGYQLLTDKELYLL"
     gene            complement(759..2054)
                     /locus_tag="PK348_03970"
     CDS             complement(759..2054)
                     /locus_tag="PK348_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HXK65406.1"
                     /translation="MYWNKDIETISRKDLEALQLKKLHKALQAASTTPFYKKVFEENH
                     INIDAIKTLEHIKDLPFTSKNDLRLSYPDGMVAVPRKEIVRMHASSGTTGKSTVIFYT
                     AKDIKDWADLVARCMVATGATPDDVFQNMMGYGLFTGGLGLHYGAELLGCMVIPAASG
                     NTLRQIQLMQDFETTIIHITPSYCLHLVEVIRAEGIDPYAMTVKKAYLGAEPYSEATR
                     KKIEEQFGIDAYNSYGLSEMNGPGVAFECTYKNGMHIWEDYYIVEIINPDTGEVLPPG
                     EEGELVLTHINREAMPIIRYRTRDLTRLIPEPCPCGRTHYRIDRIKGRTDDMLIVSGV
                     NVFPSQIETVLMKFPEVGNNYQIILDRENNLDRMHIKVELYSKLFHGDIKEIERIKNR
                     LKEALKALITINPRIELLEPGSLPPSEGKAKRVIDNRTL"
     gene            complement(2147..2995)
                     /locus_tag="PK348_03975"
     CDS             complement(2147..2995)
                     /locus_tag="PK348_03975"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HXK65407.1"
                     /translation="MQSYYHFGSAITPSVKKIIIFTSVIYIIQLIGNIITPGLIESTF
                     GIHHIGFIHQLKLWQPLTYMFLHGNFFHIFFNMFALWMFGGDLEELFGSKNFTKYYLY
                     SGIGAGLCIALMNYYIFDSYGYSPITIGASGAIYALLLAYGMIWPEREVLLYFLFPIK
                     MKYLLLIFGAIEFFGTLASASGAGGNISHIGHVGGLITGFILLKSKFWYKRVSLNPFD
                     SLFRNYRLARHKKRIEERIKAKEIIDTLLDKITRYGYGSLSPEEKRQLEWARKHFYPS
                     NNDIIH"
     gene            complement(3016..>4279)
                     /locus_tag="PK348_03980"
     CDS             complement(3016..>4279)
                     /locus_tag="PK348_03980"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HXK65408.1"
                     /translation="LTDFEILDKAVERAVESKLLKEERDRYIQQLTYLNDIIGFLNKG
                     VSLEQIFDIIASRMKKAIPFNRLAIALINDAGLVETTLVSSDKPVLIKPGATFPLEES
                     SLVLVAQTKKYDIISDLEEYSKKHNSEKARLLLQEGMQSTMTVPLVVGGRLKGFLLFA
                     SCNKNAFAPEHVQFAETISGHIVFSLQRGELLHQLDMYNKKLEEIVKIRTHQLLKTQK
                     ATIFALSSLAELRDPETGYHLERIRGYCMLLAQIAKYTGGFLEINNQFLRDLYDSSIL
                     HDIGKVGIPDSILLKPGKLTHEEFEIIKTHTTIGYNSLKRASEEMGRDSFLNMAMDVT
                     LYHHEAWDGSGYPHALSGEDIPLAARIVTIADVYDALTTVRPYKAAFDHQKAIAIMNE
                     ESHRYDPKLFELFIQNADDFDEIRNKYQ"
BASE COUNT         1206 a          866 c          838 g         1369 t
ORIGIN      
        1 ctgtttcagt tgcctgaatg cctgttgtat tgggctttgg atatccctta cacataatga
       61 cataggttga gtcatcttta aggtattttt ttatcagtac atccggcttt ccctgtatgc
      121 tgatctgctg tgaacatgaa agcaggataa aggcaaagct aataaacaat atagaaatat
      181 attgtttcat atatattcct ccaaaataaa aatggtaaga atagaaattc atataaatat
      241 aattgttgaa gtatccctgg tttcaccgcg acgactgaag aatcttgcta cctgaaagaa
      301 aatactgtct acaacaaata taattccttg tcagtcaata attgatagcc tgcatcttta
      361 agggcttttt ctgctgcttt tggattatca acctggaata caaatacaga ttcttcatga
      421 ggcttaactg taaagccata ggcatcatca acattgattg catggtcttt gagaatggct
      481 gctactttat aaaggcttcc gggtttatcg cctattttaa cagctactat ctctttcaac
      541 gtggcggcaa caccttcttc tttcagagca gtgcatccat cttcgggcct gtccaatagc
      601 agcttgataa taccataatc acctgaatca tttatggtga tagccctgat attaatattg
      661 tgctttgcca gtatacttgt tattctttca attttgccgg gtttattctc agcaaatact
      721 gatacctgga atgccatatg atacctcccc ttacagtatt atagcgtacg gttatcaata
      781 acacgttttg ctttcccctc gcttggtggc agcgaacccg gttctaaaag ctcaatgcgg
      841 ggattaatgg tgatcagtgc tttcaatgcc tctttaagcc tgtttttaat ccgttcaatt
      901 tctttaatat cgccatggaa aagctttgaa tacagctcaa ctttaatatg catcctgtcc
      961 aggttatttt ctctgtccag gattatttga tagttgttgc caacctcagg aaatttcatg
     1021 agtaccgttt caatctgtga tgggaatacg tttacaccgc tcactatcag catatcatca
     1081 gtacgtcctt taatgcggtc aatgcggtaa tgggtgcgtc cacatgggca tggctcaggt
     1141 ataagacggg taagatctcg cgtgcgatag cggatgatgg gcattgcttc acgattgatg
     1201 tgcgtaagta ccagttcccc ttcttcgccg ggtgggagta cttcgccagt atcaggatta
     1261 attatctcaa caatatagta atcctcccag atgtgcatac catttttata ggtgcactca
     1321 aatgcaacac ccgggccatt catttcactt aagccatatg aattatacgc atcaatgcca
     1381 aactgctctt cgatcttttt tcgggttgct tctgaatagg gctcagcacc aaggtatgct
     1441 tttttgacgg tcattgcata cggatcaata ccttcagccc ggattacttc caccaggtgc
     1501 aggcaatagc taggggtaat atggatgata gttgtttcaa aatcctgcat cagctgaatc
     1561 tggcgcagcg tatttcccga agctgctggg atgaccatgc aacctaacag ctctgcacca
     1621 taatgtaagc ccagcccacc ggtaaaaagc ccatatccca tcatattctg gaatacatcg
     1681 tcaggtgttg caccggttgc aaccatgcat cgtgcaacta agtcagccca atcttttatg
     1741 tcctttgcag tatagaatat tactgttgat ttgccagtag ttcccgatga tgcgtgcatg
     1801 cgaactattt ctttcctggg gacagccacc atgccatcag gatagctcaa acgaagatca
     1861 ttctttgagg taaatggcag gtctttaata tgttcaagtg tctttatggc gtcaatattg
     1921 atatgatttt cttcaaaaac ctttttataa aatggtgtag tacttgcagc ctgcaatgct
     1981 ttatgcaatt ttttgagttg caacgcttct aagtctttac ggcttattgt ttcaatatcc
     2041 ttgttccagt acatggggca cctcatggca aaaataatac aacgatggtg tcacaacaga
     2101 gcttgttgtc aagaagtaat ttatccccgg ttactatcgc caatacttaa tggatgatgt
     2161 cattattgct gggatagaag tgtttgcggg cccattctaa ctggcgtttt tcttcaggag
     2221 ataatgaccc ataaccatag cgggtaattt tatctaagag tgtgtcaatg atctctttgg
     2281 cttttatacg ctcttcaatt ctttttttgt ggcgtgctaa gcgatagttc ctgaacagtg
     2341 aatcaaaggg gtttaaggaa acacgtttgt accagaattt tgattttaat aggataaatc
     2401 cggtgataag tccacctaca tgcccaatat ggctgatgtt cccacctgcc cccgatgctg
     2461 aagctaatgt gccaaagaat tctatggctc caaatattaa taacagatat ttcatcttga
     2521 taggaaatag aaagtataaa agcacttccc tttctggcca tatcatgcca tatgcaagaa
     2581 gtagtgcata tattgcgcca gaggctccta tggtaatggg gctatatcca tagctgtcaa
     2641 aaatataata attcataagt gctatacata aacctgcacc tatcccactg tacaggtaat
     2701 atttggtaaa atttttactg ccaaaaagtt cttctaaatc accgccaaac atccacaggg
     2761 caaacatgtt aaaaaatata tggaagaaat taccatgaag aaacatgtag gttaaaggtt
     2821 gccatagttt caactgatga ataaaaccaa tgtgatgtat accaaatgtg gattcaataa
     2881 gtcccggggt aatgatattc ccaataagct gtataatata aataacggat gtaaatataa
     2941 ttattttttt tactgatgga gtaatagcgc tgccaaagtg ataatatgat tgcatacaat
     3001 tctcctataa agacattact gatacttatt gcgaatttca tcaaaatcgt ctgcattttg
     3061 tataaacaat tcaaagagtt taggatcgta gcgatgtgat tcttcattca tgatggcaat
     3121 ggctttttga tggtcaaagg ctgccttata tgggcgtaca gttgtaaggg catcgtatac
     3181 atcagcaatg gttacaatgc gggcagcaag tggtatgtct tctcccgata gtgcgtgggg
     3241 ataccctgaa ccatcccatg cttcatgatg gtacaatgtt acgtccattg ccatattgag
     3301 aaatgaatcg cgccccattt cttcagaagc ccgttttaac gaattatagc caatagtagt
     3361 gtgtgtttta attatttcaa attcttcatg ggtcagcttg cctggtttca acagtatgct
     3421 atctgggatg ccaactttgc caatatcatg taaaattgaa gaatcataca ggtcgcgtaa
     3481 aaactggttg ttaatttcta aaaatccacc agtatatttg gcaatttgtg ccagcaacat
     3541 acaataccca cggatgcgct ctaagtgata gcctgtttca gggtcgcgaa gttctgctaa
     3601 actgctcaat gcaaatattg tggctttttg tgtttttaat aactgatggg tacgtatctt
     3661 aactatttct tctaattttt tattgtacat atcaagctga tgcaacagtt cgccccgctg
     3721 caggctgaaa acaatgtggc cacttattgt ttcagcaaat tgaacatgtt ccggtgcaaa
     3781 tgcatttttg ttgcatgatg caaacagtaa aaagcctttg agcctgcctc cgactacaag
     3841 tggtactgtc atggtcgatt gcatgccttc ctgcagtagt agtctggcct tttcagaatt
     3901 gtgtttttta ctatattcct ccaaatcact gataatatcg tatttttttg tttgtgctac
     3961 cagtaccagt gatgattctt ctaaggggaa tgttgcacca ggtttaatta atactggttt
     4021 atcagaagaa accagtgtgg tttctaccag ccctgcatca ttaataagag ctatcgccaa
     4081 acgattgaaa gggatagctt ttttcatacg gctggcaatg atgtcaaaga tttgctcaag
     4141 tgaaacgcct ttatttaaaa acccaatgat gtcgttaaga taggttaact gttgtatgta
     4201 gcggtcacgc tcttccttga ggagtttact ttcaacagca cgttcaacgg ctttatccag
     4261 tatttcaaaa tcagtaagc
//
LOCUS       DAOSZP010000129         4253 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_16913_length_4253_cov_3.260124, whole genome shotgun sequence.
ACCESSION   DAOSZP010000129 DAOSZP010000000
VERSION     DAOSZP010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4253
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16913_length_4253_cov_3.260124"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1084
                     /locus_tag="PK348_03985"
     CDS             <1..1084
                     /locus_tag="PK348_03985"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HXK65409.1"
                     /translation="SDIHTIITIDESSTYTSMDSIVGSDTYDNHYVQENNDAAAIIFT
                     SAMDGYAVGAVLTHYGIYQSAKAVAQMMPGHALVSVAVLPLFHAFGLTSSLVVPLLKN
                     IPIELVEKFSLKKLIRILSNNEVRSFVGVPAMYAIMKGLFERGNNFGHIQLWVSGGDY
                     LPVSLQKWYAERQVDIRQGYGLTEASPIVSWNMPEIENKIGSIGKPMPYNKVRVVDDS
                     CRDVPACMNGEIIVKGMNVINRYYNNPQKTKEYIKDGWLYTGDIGYFDNDGYLFISGR
                     KKDMVLKNGYNVYPKEVERLLSYHPDIESVRVTGHVEFLGDSTREWLIARVKLKHHAT
                     LTADTLRMWCSENISLYKIPDEIIIE"
     gene            1852..2880
                     /locus_tag="PK348_03990"
     CDS             1852..2880
                     /locus_tag="PK348_03990"
                     /inference="COORDINATES: protein motif:HMM:NF022427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2804 domain-containing protein"
                     /protein_id="HXK65410.1"
                     /translation="MQQLISKKGDLLGKDGQLIEKGYSISPVRTYNPENVSLYPVSWL
                     NKIRIKEWDYYGITTKDYFVSATISHIGYAGLVFVYFIDFKTKQYIEDTLITPLGKGF
                     TLGRSSDDDSYFKKDDITFAFRKKGNEREIYVSWPQFGKGKGFHAHWIIRQTPHDSIV
                     MMTPIGKKRFYYNQKINCMPARGAFLLGGQEYGFDSGNALATLDWGRGVWEYKSFWNW
                     ASASWFLPDGTPFGLNLGQGFGDLSYATENCFFVNGRIHKLDKVTFNYDNSNFLKPWY
                     FGSNDGRLGLTFSPFFERVATSNLLVITSEVHQLFGIYNGFVIDNSGKKIQVANIIGW
                     AEEHHARW"
     gene            2891..3316
                     /locus_tag="PK348_03995"
     CDS             2891..3316
                     /locus_tag="PK348_03995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65411.1"
                     /translation="MSGYFTVHLPSFSLMHVHWAIDKEYWDTIVSHAVTDVYGDPVLF
                     IRIVKLHGNEWILDQQIDVFGRNNHWIVSIGDNLHGCTIKVMLMTVIEGKGEEIIFES
                     DVIMLPLSPENLKDTIPTFTQEIHTLNTIDLANEKRLSS"
     gene            3313..>4253
                     /locus_tag="PK348_04000"
     CDS             3313..>4253
                     /locus_tag="PK348_04000"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65412.1"
                     /translation="MIGDNKSTTKLCIILHAHLPYVRNPLLTLPLEEIWLYQNIAECY
                     IPLLQMCQRLIKKNIIPSVALSISPTLLTMLQQPYYHRRYQKWAHTLLDAISLLRKKN
                     TKACEALDFYNSFITHSLSFFEEINGNCADAFGELFSNNSIELLTTAGTHPFFPLYRT
                     YPSFLNLQVSSGIHTFAKQFMKLPRGFWLPELGYHPGIDKLLYQNAVGYTIVNDASVL
                     FTRNIPKTGNFFPLKTHTGLVLFPRDAVLSMKIWSSHQGYPGNPDYREFHRDAVYELP
                     ELSPNNEHRLLGLKIYAISGGNHKEYYDYKKARVVVR"
BASE COUNT         1385 a          697 c          864 g         1307 t
ORIGIN      
        1 cagcgatatt catactatca ttaccatcga tgaaagcagt acatatacat caatggattc
       61 aattgttggc agcgatactt atgacaacca ttatgttcag gaaaacaatg atgcagcagc
      121 aataatattt acctccgcaa tggatgggta tgcggtaggt gcagtactta cccattatgg
      181 catttatcag agtgcaaaag cagtagctca gatgatgcct ggacatgctc tggtttcagt
      241 ggctgtactt cccttgtttc atgcatttgg gcttacctct tcgcttgttg tgccactttt
      301 gaaaaatatt cctattgagc tggttgaaaa attttcacta aaaaaattga tacggatact
      361 gtcaaataat gaagtcaggt cttttgttgg agtgccagcc atgtatgcta ttatgaaagg
      421 cctctttgag cgtggtaaca attttgggca tatacagtta tgggttagtg gaggcgatta
      481 tcttccagtt tcccttcaaa aatggtatgc tgaacgtcag gtggatatac gtcaggggta
      541 tggactaact gaggcttcgc ccattgtaag ctggaatatg ccagaaattg aaaataaaat
      601 tggttccata ggaaaaccaa tgccctataa taaagtgcgc gtggttgacg atagctgcag
      661 agatgttcct gcttgcatga atggggagat cattgtaaag ggaatgaatg tgattaaccg
      721 gtactataat aatccacaaa agacaaaaga atatattaaa gatggatggc tatataccgg
      781 tgatattggg tattttgata atgatggcta tttatttata tccgggagaa aaaaggatat
      841 ggtattgaaa aatggataca atgtatatcc caaagaggtg gagcggttac tatcctatca
      901 tcctgatata gagagtgtac gggtaacagg acatgttgag tttctgggcg atagcaccag
      961 agagtggctt attgcacgtg taaaactaaa acaccatgca acgttaacgg cggatacact
     1021 cagaatgtgg tgcagtgaaa atatttcctt atataaaatt cctgatgaga taataataga
     1081 ataaaaaaaa gaggtaatcg ggcgattacc tctagctcta caaaagcata gcggaatact
     1141 ttggaaggaa attgttgtgt accaggattt tcccagctca ctattatata taagcaaatc
     1201 gtatgccagc gtcaagtaaa aaatctcaaa ttttttatat aaatacaaaa atttttaaat
     1261 tttaagcata tttcatacaa ttttaaagaa tgtttagtac tttaaggtaa aaaccagaag
     1321 cgcataaaga atagattatg tgcataaaat cttaaaatgt cacgtatatg cattgtatgt
     1381 atacgttttt aactataaaa gtgtatggaa aaacggacac atgtttagta taccgtacat
     1441 acggactatt ttattcaatg tggtgaatgg attttcaatt agatgaaata tttatgccat
     1501 aagtcatcct gaatacagcc tttttgccat aaaggatttg attctgaaac acaaagtatt
     1561 caaagatatg aatgattcta tgatggataa taatgaaatt ctattttaag aacagttgcc
     1621 aaacgactgc ttgcataccc atgctgaaaa gtcttaataa atgtaagaca ataatctaaa
     1681 ttgtttatgt catcacatca ggttgaaatt tatcagaatg atatttaaaa ctagataatt
     1741 tgttttaaga tatttgctta taattgcaac ttttataagt ataaaacttc ccaaataatt
     1801 gcttgactaa tgaaatttta ttacatagta tttcaaaaaa gaggtaatgc tatgcaacaa
     1861 ctcatatcta aaaaaggtga tttgcttggt aaagatggcc aacttataga aaaaggttat
     1921 tcaatcagcc ctgtcagaac atataatcct gaaaatgttt ccctgtatcc tgtttcatgg
     1981 ttaaacaaaa tacgtataaa ggaatgggac tattatggca taaccactaa agattacttt
     2041 gtttcagcta ctatctcaca tattggatat gcaggacttg tgtttgtgta ttttattgat
     2101 tttaaaacaa aacagtatat tgaagataca ctcataacgc cattagggaa agggtttaca
     2161 cttgggcggt caagtgatga tgatagttac tttaaaaaag atgatataac ctttgctttc
     2221 aggaaaaaag ggaatgaacg cgagatatat gttagctggc cgcaatttgg taaagggaag
     2281 ggttttcacg cacactggat aatacggcaa acaccgcacg attcaatcgt tatgatgaca
     2341 cctattggaa aaaaacgctt ttactacaat caaaaaatta actgcatgcc agcacgtggt
     2401 gcatttttac tgggtggaca ggaatatgga tttgactctg gtaacgcact ggcaacgtta
     2461 gactggggaa gaggagtatg ggaatataaa agcttctgga actgggcaag cgcttcctgg
     2521 tttttacctg atggaactcc gtttgggtta aatttagggc aagggtttgg tgatttatca
     2581 tatgccacag aaaattgttt ttttgtcaat gggcgtattc ataagttaga taaggttacg
     2641 tttaattatg acaattcaaa ttttttgaaa ccatggtatt ttggatccaa tgatggaagg
     2701 ttaggattaa cgttttcacc tttttttgaa agagtagcaa catccaatct tctggttata
     2761 acgagtgagg tgcatcagct atttggcata tacaatggct ttgttattga taacagcggt
     2821 aaaaaaatac aggtggccaa tattattgga tgggccgaag aacatcatgc acggtggtga
     2881 agggggaaat atgtcgggat attttacagt acatttacct tcgttttcat tgatgcatgt
     2941 gcactgggca atagataaag agtactggga tactattgtt tcgcatgcag ttactgatgt
     3001 gtatggcgat ccggtgttat ttatacgtat tgttaaattg catggaaacg agtggatact
     3061 ggaccagcaa attgatgtgt ttgggaggaa taaccactgg attgtatcca ttggtgataa
     3121 tcttcacggt tgtaccatca aagtaatgct catgaccgta atagaaggga aaggtgaaga
     3181 aattattttt gaatctgatg ttatcatgct gccgctttca ccggaaaatc tgaaagacac
     3241 catcccaaca tttactcagg aaattcatac attaaatact attgatctgg ccaacgaaaa
     3301 acgcctttct tcatgatcgg tgataataaa tctactacaa aactatgcat catactgcat
     3361 gcgcatctgc cgtatgtcag aaatcctctt ttaacactac ctcttgaaga aatatggtta
     3421 tatcagaata ttgctgaatg ctatattccc cttttacaga tgtgccagag gctcattaaa
     3481 aaaaatatca ttccatcagt tgccctatct ataagcccca cattgctaac catgttgcag
     3541 caaccctact atcacaggcg ctatcaaaag tgggcacata cccttcttga tgccatttca
     3601 ctactaagaa aaaagaatac aaaggcgtgt gaggctcttg atttttacaa ttcgtttata
     3661 acacattccc tttcattttt tgaagaaata aacggaaatt gcgctgatgc ttttggtgaa
     3721 ttgttttcta acaattcaat tgaattgcta actactgcag gaacacaccc tttttttcct
     3781 ttatatagaa catatccttc attccttaat cttcaggtca gtagtggaat tcatacattt
     3841 gcaaaacaat ttatgaaatt accacgggga ttctggctgc ccgagttagg ataccatcca
     3901 ggaattgata agctgttgta tcaaaacgca gtaggctata ctattgttaa tgatgcaagt
     3961 gttttgttta ctcgaaatat tccaaaaaca ggaaattttt ttcccctgaa aactcacacg
     4021 gggctggttt tgtttccccg ggatgcggtt ttatcgatga agatctggtc gtcgcatcag
     4081 gggtatccgg ggaaccctga ctacagagag ttccataggg atgcagtgta tgaattgccg
     4141 gaattatcgc ccaataatga acatagacta ttggggttaa aaatttatgc gataagcgga
     4201 ggcaatcaca aagaatatta tgattacaag aaagcacgag tggtagtgcg cca
//
LOCUS       DAOSZP010000130         4250 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_16926_length_4250_cov_3.237902, whole genome shotgun sequence.
ACCESSION   DAOSZP010000130 DAOSZP010000000
VERSION     DAOSZP010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4250
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16926_length_4250_cov_3.237902"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..839)
                     /locus_tag="PK348_04005"
     CDS             complement(<1..839)
                     /locus_tag="PK348_04005"
                     /inference="COORDINATES: protein motif:HMM:NF013507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HXK65413.1"
                     /translation="MNFKILYKYGVTVLLITGLIVFATSLLIGFNEKESSFTRYLLHA
                     PFSGRAGHTCTVFKGKLWLIGGWDGNNPYNDVWYSKEGLHWYSAGPMPFKPRAGHTAF
                     VYNDKLYIVGGLTFDAARNIIDLNDIWATEDGISWQCVKETAEFMPRGGHACTVFNNT
                     ILLVGGIACGAEAFKSDDGINWHKIAGNLPFGSRGGHVLTAFNNKLWVIGGIRVDGNN
                     NFTSFSDVWNSYDGIHWKKVASNMHFFAGGGHGAVVYKKSLWVIGGFRKGGTVYRSDN
                     GKD"
     gene            complement(872..2344)
                     /locus_tag="PK348_04010"
     CDS             complement(872..2344)
                     /locus_tag="PK348_04010"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HXK65414.1"
                     /translation="MNTILEKYYRVLIADDEKGIRDLLKDLLEGSFYVSTIDRGDTII
                     EEITKNKYDILILDLQMPGKNGIEVLQTIRDLKLDIVVVVITASNDVQLAITSMKLGA
                     YDYLVKPFDTDKLLVILKNIVEKLDLENEVNELREKVGESFRFRNIIGKSPKMQRIFA
                     TLERVIDTDSTILIIGESGTGKEIIAKAIHYNSNRKQYPFKSIDCSTIPQDLIGSELF
                     GHEKGAFTGAIARKIGKFEVASKGTLFLDEISNLSFDMQAKLLRVLQEKEFERIGGNE
                     IIKVDTRIVAASNRDIREMVKQGTFREDLYYRLNVVPIYLPPLKERKEDIPLFIDYFL
                     NKFTTEYGRDISLDLQSRLYLTDYHWPGNVRQLENVIKRLVLLSTEHVVKLDIVKSVL
                     EFEEYNKPKEKTVNVENEHINHTIPEAFVNSKKVQDSESHVDRVKTLDEMEREYIEKM
                     LKHFDYNISLTAKAINVSRKTMHNKLKKYNIAIKKTVIEN"
     assembly_gap    2471..2480
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2481..3008)
                     /locus_tag="PK348_04015"
     CDS             complement(<2481..3008)
                     /locus_tag="PK348_04015"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HXK65415.1"
                     /translation="MHEVDIIERVIRNIAHEVRNPLTTVKGYAQLLSQKNEPALIVKS
                     QHMIIEQSERIDTIFTQLYDAFTVIDDDRAECTFPEVLYTVVEESPYSEYITIERNAQ
                     FRISLYVNRFQQCLSSIINGFNWKYFSGASLHINALKDMHCIVNISFSGVVFDSIPED
                     IFYYPFQCKQFFSKGT"
     gene            complement(3016..3399)
                     /gene="rsfS"
                     /locus_tag="PK348_04020"
     CDS             complement(3016..3399)
                     /gene="rsfS"
                     /locus_tag="PK348_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012994942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HXK65416.1"
                     /translation="MTGKSKRTKSKITVDPEVLSLMKGCKKVLDDTKAIDIVFLDLRD
                     VNSYLDYFLICTGNSILHCKALAREVHKYFLEKGIKERSRTTTNSPWIVLDYNDIIIH
                     IFTKETREFYQLERLWADAKQIELD"
     gene            complement(3396..>4250)
                     /locus_tag="PK348_04025"
     CDS             complement(3396..>4250)
                     /locus_tag="PK348_04025"
                     /inference="COORDINATES: protein motif:HMM:NF024791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HXK65417.1"
                     /translation="TLNKRTGKAKRLESLSFSDYSDIIDSLEDDLHITIPFYIELYAS
                     DAIKIIDLIEGINLYILDQVKMDLGYTTGINYCDGRKALWYINKVEQNSIYLKYDRIM
                     DVALTLFTNKDVYKKYAKKEYISEMVSSINTNLLLQEIISLIDVVYDARTVYTTIMPG
                     KMDKDNNYTVDDITRKLYEQKFLRPLVLDIKDSESIKVRILNGSDIPGLARKMRNVLT
                     REGLTVVEFGTSPYEVTDKTVIINQKGNLNSALKVSSILGIDTMYHIVDSSQLADVLV
                     IVGKDYSQ"
BASE COUNT         1300 a          876 c          614 g         1450 t
ORIGIN      
        1 caatcctttc cattgtctga cctgtaaaca gtaccccctt ttctgaatcc acctataacc
       61 cacaacgatt ttttatatac cactgctcca tgcccaccgc cagcaaaaaa atgcatattg
      121 cttgcaactt ttttccagtg aattccatca tatgaattcc acacatcgct gaagcttgta
      181 aaattattat ttccgtcaac acgaatacca ccaataaccc ataatttatt gttgaatgca
      241 gtcaaaacat gtcctccacg tgaaccaaag ggcaaattac ctgcaatttt atgccagtta
      301 ataccatcgt ctgatttaaa agcttctgca ccacaggcaa tacccccaac cagcaaaata
      361 gtattgttaa atactgtaca tgcatgtcca ccacggggca taaattcagc ggtttctttt
      421 acacactgcc agctaatacc atcttctgtt gcccatatat cattcaggtc aataatattt
      481 cgtgccgcat caaacgtaag cccgccaact atatacaatt tatcattata tacaaatgca
      541 gtatgtcctg ctcttggttt aaaaggcatt ggcccggcac tataccagtg taaaccctct
      601 tttgaatacc atacatcatt atacggatta ttgccgtccc agccacctat aagccataat
      661 ttacctttga agacagtaca ggtatgaccg gctctaccgg aaaatggcgc atgcaataga
      721 tatcgagtaa agcttgattc tttttcatta aaaccaatta gcagtgaagt agcaaacaca
      781 atcagccctg taattaacag tactgttaca ccatatttat ataaaatttt gaaattcata
      841 cttattctct gagattacct gacaatatta ttcaattttc tattacagtc ttttttatcg
      901 ctatattata ttttttcaat ttattatgca ttgttttgcg tgatacatta atggcctttg
      961 ctgttaagga aatattataa tcaaaatgtt tcaacatttt ttcaatatat tctctttcca
     1021 tttcgtcaag tgttttaacc ctatcaacgt gactttcgct atcctgcact tttttgctat
     1081 ttacaaaagc ctcaggaatt gtatggttta tgtgttcgtt ttcaacattc acggtttttt
     1141 cttttggttt attatattct tcaaattcta acacactttt aacaatatca agcttaacaa
     1201 catgttccgt tgaaagcaac acaagccttt taataacatt ttccagttgc cgcacattgc
     1261 caggccagtg ataatctgtc aaatataatc gtgattgcaa atcaagtgaa atatcccgac
     1321 catattcagt tgtaaattta ttcaggaagt aatcgataaa aagtggtata tcttcctttc
     1381 gctctttcaa cggaggcaaa taaattggca ccacatttaa acggtaatat aaatcttccc
     1441 tgaatgtacc ctgcttaacc atctcgcgta tgtcacgatt acttgcagca acaattcgtg
     1501 tatccacttt aataatttcg ttgccgccaa tacgctcaaa ttctttttcc tgcaatactc
     1561 gtaataattt tgcctgcata tcaaatgaaa ggttgcttat ctcatccaga aaaagagtcc
     1621 ctttgctggc aacttcaaac ttaccaattt ttcgggcgat agctcctgtg aatgctccct
     1681 tttcatggcc aaaaagctca ctacctatca aatcctgtgg tattgtgctg cagtcaattg
     1741 atttgaaagg atattgtttg cgattgctat tataatgaat tgcttttgca attatctctt
     1801 taccagttcc actttcacca ataattaaaa tggtactatc ggtatcaata acccgttcca
     1861 acgttgcaaa gatacgctgc atttttggcg acttgccaat aatatttcta aacctgaagc
     1921 tttctccaac tttttcgcga agctcattga cttcgttttc cagatcaagc ttttcaacta
     1981 tatttttgag tattaccaga agtttatcgg tatcaaatgg ttttaccaga taatcatagg
     2041 ctcctaattt catggacgta attgccagct gcacatcgtt tgaagcggtg attaccacta
     2101 ctacaatatc caattttaaa tcacgtattg tctgtaacac ctcgatgccg tttttaccag
     2161 gcatctgcag atcaaggatt aatatatcat atttattttt tgttatttcc tcaattatgg
     2221 tatcaccacg atcaatggta ctgacataaa atgagccttc gagcaggtct tttaacaggt
     2281 ctcgaatacc tttttcatca tcagcaataa gcactcggta atatttttca agaatggtgt
     2341 tcatggtcac agcattaatt atttatgaag aatagttaca ttggaatctc aagagtaaat
     2401 ccattacgct cttttaaaaa tgtaaattgc catccactgc gctgtgcaat gcaatacact
     2461 ttaaaaagct nnnnnnnnnn cgtcccctta ctgaaaaact gcttacactg aaatggataa
     2521 taaaaaatat cttcagggat actatcaaac acaacacctg aaaaactgat attgacaata
     2581 cagtgcatat cttttaaggc attaatatgt aacgatgcac cagaaaaata tttccagtta
     2641 aaaccattaa tgatgctgct caggcattgt tgaaatctat tgacatacag tgatatcctg
     2701 aattgggcat tgcgctcaat tgtaatatac tcactatatg gagattcttc aaccaccgta
     2761 tacaaaactt caggaaacgt gcactctgca cgatcatcat ctatcacagt aaatgcatca
     2821 tacaactgtg taaaaatagt atcaatgcgt tcagattgct caataatcat atgctgtgac
     2881 ttaacaatta aggcaggttc atttttttgc gatagtaact gggcatatcc cttcactgtg
     2941 gttaaagggt tgcgcacttc atgagcaata tttcgtatta ctcgttcaat gatatcaact
     3001 tcgtgcatgt gtgatttaat ctaattcaat ctgcttagca tccgcccata gacgctctaa
     3061 ctgataaaat tcacgtgttt cctttgtaaa tatatgaata ataatatcat tataatccag
     3121 aacaatccat ggtgaattgg ttgtggttct tgaacgctct ttaatgccct tttcaagaaa
     3181 atatttatga acttcacgtg ccagtgcctt gcaatgtaat atagaattac cggtacatat
     3241 taaaaaataa tctaaatagg aattcacatc ccttaaatcc agaaagacga tatcaattgc
     3301 ttttgtatca tcaagtacct ttttgcatcc tttcatcagg gataaaactt cgggatctac
     3361 cgtaattttt gattttgttc ttttactttt acctgtcact gtgaataatc ctttcctaca
     3421 attactaaga catctgctaa ctgcgatgag tcaactatat gatacatggt atcaattcca
     3481 agaattgaag atactttcaa tgctgagttt aaattccctt tttgatttat tatcacagtt
     3541 ttatcagtta cctcatatgg tgatgtgcca aactcaacca ctgtaagccc ttcacgcgtc
     3601 aatacattgc gcatctttcg agcaagcccg ggaatatctg agccatttaa tattctgacc
     3661 ttgattgatt ctgaatcttt aatatcaaga accagtgggc gtaaaaactt ttgttcatat
     3721 aattttctgg taatatcatc caccgtataa ttattgtcct tgtccatctt gcctggcata
     3781 attgttgtat atacagtcct ggcatcatac acaacatcaa tcagggatat tatttcctgt
     3841 aataataaat tggtatttat tgaacttacc atttcactga tatattcttt ttttgcatat
     3901 tttttgtaca catctttatt tgtaaaaagg gtaagggcaa catccattat tctatcatat
     3961 tttagatata ttgaattttg ttcaacttta ttaatatacc ataaggcctt acggccatca
     4021 cagtaattaa tgccagttgt atatcccaaa tccattttca cctggtcaag tatatataaa
     4081 tttattcctt caattaaatc aataattttt atggcatctg aagcatacaa ttcaatgtaa
     4141 aatgggattg taatatgaag gtcatcttca agtgagtcta ttatatcact gtaatcagaa
     4201 aacgaaagtg actcaagcct ttttgctttt ccggttcttt tgttaagagt
//
LOCUS       DAOSZP010000131         4234 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_17006_length_4234_cov_3.306054, whole genome shotgun sequence.
ACCESSION   DAOSZP010000131 DAOSZP010000000
VERSION     DAOSZP010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4234)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4234)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4234
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17006_length_4234_cov_3.306054"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /locus_tag="PK348_04030"
     CDS             complement(<1..254)
                     /locus_tag="PK348_04030"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HXK65418.1"
                     /translation="MKYDPYSPFYKPEHYRGSFVLNRKRGYCVSKASLLCALGRACNI
                     PSRVGFATVKNHIATKQLLETLGSNIFVYHGFTEFYLNGK"
     gene            complement(535..1173)
                     /locus_tag="PK348_04035"
     CDS             complement(535..1173)
                     /locus_tag="PK348_04035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65419.1"
                     /translation="MQNTFSEYDIFRYIPDELDFTIHNPYLQEILQYKDKHLDEYQYW
                     NLRYELIKKYAFAVPTFQLLSIIQELSPIIEIGAGNGYWAYMLKQVGVDVVAFDKYPP
                     DDAQYPFVNHSDNVWFDEQWYDVQKGDALEAGQYPERTLFLCWPQPQSTMALEALQAY
                     KYAGGHTVVFIGDMIACAENRFYDMLAECTLKLKKQIWGYKYFNEIIAVYYL"
     gene            complement(1194..1267)
                     /locus_tag="PK348_04040"
     tRNA            complement(1194..1267)
                     /locus_tag="PK348_04040"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1231..1233),aa:Arg,seq:tcg)
     gene            complement(1586..1657)
                     /locus_tag="PK348_04045"
     tRNA            complement(1586..1657)
                     /locus_tag="PK348_04045"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1623..1625),aa:His,seq:gtg)
     gene            complement(1770..2804)
                     /locus_tag="PK348_04050"
     CDS             complement(1770..2804)
                     /locus_tag="PK348_04050"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXK65420.1"
                     /translation="MLYRIWTIFIARTKEFYRDRSALGWNIIFPLLIIIGFSVVFRSE
                     SSPVLKVGVVKPLNNEITIISNKQFNEFLKLKYIDFIEFSSKEKAVDSLQKFKIDMLV
                     DPEHNTYFINDSSPNGYIAEQLLKGAGTTTTFFSKHSFKGRGIRYVEWLFPGILGMNA
                     MFNSLYGVGYVLVMYRKNGILKRFSVAPVRPFEFLTAQILSRMFVMLATTIVVYIGTV
                     LLYGFQCKGSLFTLFVVFALGGFCMISLGLLIACRSDSQEFADGLINIITWPMMFLSE
                     VWFSLEGARPWVQKVSQLLPLTPIVESARMVMNEGATLFDIRYQLLYLIVVSAIFLLS
                     GSLLFRWQRK"
     gene            complement(2807..3691)
                     /locus_tag="PK348_04055"
     CDS             complement(2807..3691)
                     /locus_tag="PK348_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015281936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXK65421.1"
                     /translation="MDYILEVKNLYKYYPGVKAVDDVSFAIPRGICFGLLGPNGAGKT
                     TTIEVIEGIQKPDNGTILYKGKPRDQHFKQEVGIQLQHTELQQYLTVKEILLTFRNLY
                     ARRASLDELVSLCYLEEILDRDNRKISGGQKQRLLLAIALANDPELLFLDEPTTGLDP
                     QARRHVWDIVQSIKKKGKTIILTTHYMEEAQTLCDIVAIMDRGKIIAMDSPINLLNEH
                     CRGVTITLPSTVDENILRQMNCEWYTVNHTIEIHTEQPHDCLRLLIDNNVDITGMVVR
                     SQNLEDLFLKLTGTQLRA"
     gene            complement(3764..>4234)
                     /locus_tag="PK348_04060"
     CDS             complement(3764..>4234)
                     /locus_tag="PK348_04060"
                     /inference="COORDINATES: protein motif:HMM:NF024487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HXK65422.1"
                     /translation="EIVKLSNRLIAKNRFRSRRWVKSVRGGGGKIYFVKVDSFEIEAL
                     IADTIAKQYDKKISIGVLYRNNWQGTFLQSRINTDDNVKFMTIHGAKGLEFDVVILCG
                     VKDRLLPDPYTDIEEERRLMYVALTRAKNCLHILYHPAYSNPKPQFIEECESYV"
BASE COUNT         1340 a          892 c          678 g         1324 t
ORIGIN      
        1 cattttccgt ttaaataaaa ctcagtaaaa ccatgataca caaatatatt acttcccaat
       61 gtttccagaa gttgctttgt tgctatatga tttttaacag ttgcaaagcc tactctgcta
      121 gggatattgc aggcacgtcc taaagcacat aataaagagg cttttgatac acagtatcca
      181 cgcttgcggt tcaatacaaa actgcctcta taatgttcag gcttataaaa cggagaatac
      241 ggatcatatt tgatatcatc acggacagca taaaaaagct ttactgccta tttaacagca
      301 tcggtttcac tgccaataat agtgcgagcg tagtcaagaa catcagggct atcgctatca
      361 atgcattgtg ttggcgtaag atatttttcc aattgctttt cagtcatata agtagtatgt
      421 ggttataaat ttaggtaata gtataaataa gtatagtcca aatcatgttt ccttcaataa
      481 ttattgtcaa tcctaaatac tataaatgta atgtcaaaga aatagtatct tggattatag
      541 ataatacact gcaataattt cattaaaata cttatatccc caaatctgtt ttttcaactt
      601 taacgtacac tctgcaagca tatcataaaa cctgttttca gcacaggcaa tcatatcccc
      661 aataaaaact acagtatgcc ccccagcgta tttataagcc tgtaatgcct ctaaagccat
      721 ggtactttgt ggctgaggcc agcataaaaa gagtgtccgt tctggatact gtcctgcttc
      781 cagtgcatca cctttttgaa catcgtacca ttgttcatca aaccacacat tatctgagtg
      841 attaacaaac ggatattgag catcatctgg gggatattta tcaaatgcaa ccacatcaac
      901 acccacctgc ttcagcatat aagcccagta tccattgcct gcacctattt caattatggg
      961 cgatagttcc tgtataatag acagtaactg aaaggttggt actgcaaatg catacttttt
     1021 tatcagttca tagcgcaagt tccagtactg gtattcatcc aggtgcttat ctttatactg
     1081 tagtatttcc tgtaaataag ggttatggat agtgaagtct aattcatcgg gaatatagcg
     1141 gaatatatca tattcactga aagtgttttg catggtgaat atactgtatg gtgcgcgccc
     1201 ggcaggagtc gaacctgcgg cctgcggatt cgaagtccga cgctctatcc agctgagcta
     1261 cgggcgcgag ttgattttaa aaaaatttaa aaaatttttt aaaatgtttt tcaaacattt
     1321 tatcctgtta ctgagctacg ggcgctttca ttgttataac aaatttaacg aagcacctgt
     1381 tacttctttg ttacttgctt aaatcatctt ttaaatgttt ttcaaacatt ttatcctaaa
     1441 ctgaactacg ggcgcgctta ttatgatgct gaatattatt caatatttct tgcccatttg
     1501 atgttttttt tcaaacaatt ttttaaaatg tttttcaaac attttactct acaactaaac
     1561 cacaagcgcg agtaaaataa aaatgggtgg atgacggggc tcgaacccgc aacaactgga
     1621 tccacagtcc agggctctac cattgagcta catccaccgt aactatctga caaataaact
     1681 ataattagga atgacaatac ttaaaatgta attatagtgt caattatatt tcaactcaaa
     1741 attttttcaa ttttacgtaa taaatactac tactttcgct gccatctgaa aagaagcgaa
     1801 cctgataata aaaatatagc agataccaca atcaaataca ataactgata gcgtatatca
     1861 aatagtgtgg caccttcatt catcaccata cgtgcacttt ccactatagg tgtaagcggc
     1921 aaaagctggg ataccttctg tacccaggga cgtgccccct ccagtgaaaa ccacacctct
     1981 gatagaaaca tcatgggcca ggtaataata ttaattaacc catcagcaaa ctcctggctg
     2041 tcacttcgac aagcaatcag caaacccaac gaaatcatac agaacccacc cagtgcaaaa
     2101 actacaaaca gcgtaaataa cgaacctttg cactgaaatc catacaataa aacagttcca
     2161 atatacacaa caattgttgt tgcaagcatt acaaacatac gcgacaatat ctgagctgtt
     2221 aaaaattcaa atggtcttac aggtgcaaca ctgaatcgtt ttaatatgcc gttttttcta
     2281 tacattacca ggacataacc tacaccatat aatgagttaa acattgcatt cattcctaaa
     2341 atgccaggaa aaagccattc aacatagcgg attcccctac ccttaaacga atgtttagaa
     2401 aagaatgtag tagttgtacc tgcgcctttc aacagttgtt ctgctatata cccattgggt
     2461 gatgaatcat tgataaaata cgtattatgc tcaggatcca ccaacatgtc tattttaaat
     2521 ttttgtaaac tatcaacagc tttttcctta gatgaaaact caataaaatc tatatacttt
     2581 aattttaaaa actcattgaa ttgtttatta cttataatcg taatttcatt attcaatggc
     2641 ttcaccacac caactttgag tactggcgat gattcgcttc tgaatacaac actgaaacca
     2701 ataataatca aaagcgggaa aataatattc cacccaagag ctgacctgtc gcgataaaat
     2761 tcttttgtac gtgcaataaa tattgtccat atcctgtata gcattgttaa gcccttaatt
     2821 gtgttcctgt taattttaaa aacaagtcct ctaaattttg tgaacgtact accatgccag
     2881 taatatcaac attgttatca ataagcagcc tcaggcaatc atgcggttgt tcggtgtgta
     2941 tttcaatggt atgatttacc gtataccact cacaattcat ctggcgtaaa atattctcat
     3001 caaccgttga aggcaatgta atggtaactc cacggcaatg ttcattaagt aaattaatcg
     3061 gcgaatccat ggcaataatt ttaccacgat ccataattgc cacaatatca catagcgttt
     3121 gggcttcctc catatagtgt gttgtgagta ttatggtttt gcctttcttt tttattgatt
     3181 ggacaatatc ccacacatga cgtcgcgcct gaggatcaag gcctgtggtt ggttcatcaa
     3241 gaaacaatag ttcaggatca tttgctaaag ctatcgccag aagtaaccgc tgtttttgac
     3301 cgcctgatat tttcctgtta tccctgtcca gtatttcttc taaataacac agagaaacaa
     3361 gctcatccag cgaagccctg cgtgcataca aatttctgaa agtcagtagt atttctttga
     3421 cggtaagata ctgttgcaat tcagtatgct gaagctgaat gccaacctcc tgtttaaaat
     3481 gctgatcacg aggcttcccc ttatacagta ttgttccgtt gtcaggtttt tgaattccct
     3541 caataacttc aatggtagtt gtcttgccag caccatttgg tccaagcaat ccaaaacata
     3601 taccacgggg aatagcaaaa ctgacatcgt caacagcctt aacaccaggg taatatttat
     3661 atagattttt aacttctaat atatagtcca ttgcttatcc ataggtatat tgcatttgta
     3721 tcactaagca gttattttac tttgtacgct ttacatgaat acatcataca tatgattcac
     3781 attcttcaat gaattgaggt ttggggttac tataggcagg atggtacagt atatgcaagc
     3841 agttttttgc acgggtaaga gcaacataca tcaaccgccg ttcttcttct atatccgtat
     3901 atggatcggg caacaatcta tcttttacac cacacagtat tactacatca aactccaggc
     3961 cctttgcccc atgtatggtc ataaacttca cattatcatc tgtatttatc cttgactgca
     4021 aaaatgtgcc ctgccagtta tttctataca gcacaccaat agaaattttt ttatcatact
     4081 gcttagcgat agtatctgca atcagtgcct ctatttcaaa tgagtcaacc ttgacaaaat
     4141 aaattttgcc accgccacca cgcactgact taacccagcg ccgtgacctg aatctgtttt
     4201 tagcaataag tctgtttgaa agcttcacta tctc
//
LOCUS       DAOSZP010000132         4224 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_17058_length_4224_cov_4.974814, whole genome shotgun sequence.
ACCESSION   DAOSZP010000132 DAOSZP010000000
VERSION     DAOSZP010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4224
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17058_length_4224_cov_4.974814"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(273..1007)
                     /locus_tag="PK348_04065"
     CDS             complement(273..1007)
                     /locus_tag="PK348_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007547450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HXK65423.1"
                     /translation="MIDILISAFLLSIVLVGIHAYFGTEIIRRGIIFTDLAIGQMAAM
                     GAAISILLFDSNFMYVFSLSFAILGGSIISFTSKRSLYPEAIIGLLYAFSLSAVFLIL
                     SKSSHGTEEFNNLLATDILFTPLSKILETALLYSAIGLLLFFIYRRGATKCNDLLFFT
                     LFAITVTSSVKLAGVLVVFALLLSPVMIALQLKQKHILITSWITGIIINLISIGISYT
                     LDLPTGYTLVAVNTLAALIISVLKTT"
     gene            complement(1004..1924)
                     /locus_tag="PK348_04070"
     CDS             complement(1004..1924)
                     /locus_tag="PK348_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012460202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HXK65424.1"
                     /translation="MKYKNFSKIQLILIHTLVLLHLFLINTAAWAKKPVIVTTYQYIA
                     SITEQITGNSATVIALAPPQFDPHYIVPKPSYIAKLRKADLLIINGAQLEIGWLPQLI
                     RQANNPAIITGTKGFLDLSNFVKKIDVPTAISRAMGDVHPEGNPHFYLDPHNIPVLAK
                     AIYERLCTIVPKNYNLYSKNYTTFIEKFNAKLTVWDTQMTKVKGKSAIEYHKNYDYLL
                     IRYSIHLAGTIEPVPGIPPTSKHIAKLEKTIYTVSFILQNVYHPYDAVQYLSQKYSIP
                     VIVLPHDVGATSDSNDIFSLFDSIVGRITQ"
     gene            complement(2080..3354)
                     /locus_tag="PK348_04075"
     CDS             complement(2080..3354)
                     /locus_tag="PK348_04075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65425.1"
                     /translation="MKQFMQIVYMLFFISLLLQKGYPQTMADWQTSDPFSQAKFVPDI
                     AAIVDVTYVQRSLDDVTHYEIPGFKDAHSKPSNHENHEDELNQNNGFNLNYVEISLYS
                     QVDPFFDMFAVLHASTEGAEIEEAYFTTQQLPFGFKIKGGKFLSSIGRLNDKHAHDWD
                     FADLPLVYKAFFGSQLNEIGIQLNWIVPLPFYMLIGAEALRGENETSFGTNGFTVENI
                     TTIDDANKPNVYTAFVKSSFDIGNYTVLAGVSGIYGTSRIDHGIGNDTNVAGDHGFYG
                     KTYMAIGDITIRYDFNSYQYIYLQTEYIYRNQDGDLYSYDGSINSKSNFDAKQSGLYT
                     QVVARLNQLWRIGLRYDLLAQNDVSTNHDLTNNLPRYSSMIEYNPSEFSRFRLQYNYD
                     KSLYSESGTNKPNHEIIVQANITIGAHGAHSF"
     gene            complement(3944..>4224)
                     /locus_tag="PK348_04080"
     CDS             complement(3944..>4224)
                     /locus_tag="PK348_04080"
                     /inference="COORDINATES: protein motif:HMM:NF024585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HXK65426.1"
                     /translation="PAEMVFSNYFAEVNDNCTSCGSCADICQMEAIEMNDFAHVNKDR
                     CIGCGLCVSACHADAIHLVLKDKKRIPPKTSGEQMKEMAIQRGILPDG"
BASE COUNT         1349 a          713 c          774 g         1388 t
ORIGIN      
        1 tgaatttcgc gggggatata ttcacctatg tcaatgttag agaaaaacct ctttttatgt
       61 tccagtaata attcatgtag tttatttttt tccattaata gtatattcca atagtatatt
      121 ctaatagtat attctaatag tatatttcaa atgttatatt ttgcattata tattacatta
      181 gtaatgtaat aatattgact aaaattgtca ggtatttttc atacaatggc atgaatgcat
      241 gcctattaca ttgtttatgg aatcaattgg tttcatgtcg ttttcaatac actgattatg
      301 agagcagcca gtgtattgac tgcaaccagt gtataacctg ttggtaaatc cagtgtatac
      361 gaaataccta ttgaaatcag gttaatgatt atgccagtaa tccatgatgt aatgagaata
      421 tgcttttgtt taagctgtag tgcaatcatt accggtgaga gtagcagtgc aaatactacc
      481 agcactccgg ccaatttaac ggagcttgtt acagtaatgg caaataatgt aaaaaaaagc
      541 aggtcattac attttgtagc accacgtcta tagatgaaaa agagtaataa gcctattgct
      601 gagtataata gcgctgtttc cagtatcttt gacagaggag taaataatat atccgtggct
      661 aaaagattgt taaattcttc ggtaccatgt gatgatttac tcaagatcaa aaaaacagca
      721 gaaagtgaaa atgcatataa aagtcctata atggcttcag gatataatga acgtttactt
      781 gtaaatgaga taatggaccc tccaagaatt gcaaaagaaa gagaaaatac atacatgaaa
      841 ttgctgtcaa ataaaagtat tgagatggct gctcccatgg cagccatctg tccaattgca
      901 aggtcagtga atatgattcc acggcgtatt atctcggtac caaaataggc gtgtattcca
      961 acaagaacta tcgaaagaag gaaagcagat atcaatatat ctatcattgt gttatcctcc
     1021 ccacaattga gtcaaaaaga ctaaatatat catttgaatc cgatgttgca ccgacatcat
     1081 gcggcaatac tattacaggt attgaatatt tttgggaaag atattgtact gcatcataag
     1141 gatgatatac attctgtaaa ataaatgaaa cagtataaat agttttttct aattttgcaa
     1201 tgtgtttaga tgttggagga ataccgggaa ccggttcaat agtaccagca aggtgtatgc
     1261 tgtatcgtat taaaagatag tcgtaatttt tgtgatattc aatagctgat tttcctttaa
     1321 cttttgtcat ttgtgtatcc cacacggtaa gcttggcatt aaatttttca ataaaggtag
     1381 tatagttttt ggaatacagg ttataatttt taggaactat cgtacacaaa cgttcgtaaa
     1441 ttgcttttgc aagtacaggt atgttatgag ggtctaaata aaaatgtggg ttgccctctg
     1501 gatgaacatc gcccattgcg cgggatatgg cagttgggac atctatcttt tttacaaaat
     1561 ttgaaagatc cagaaatcct tttgtgccag ttataatagc aggattgttt gcctgccgta
     1621 ttaactgtgg caaccagcca atctcaagtt gtgctccatt aataattaaa agatcagctt
     1681 tccgcagttt tgcaatatac gagggctttg gtacaatata atgaggatca aattgtggtg
     1741 gtgctaatgc aatgaccgta gcactgttac cggttatttg ctctgttatg cttgctatat
     1801 actgataggt ggttactatc acaggctttt ttgcccatgc tgcagtgttg attaaaaata
     1861 aatggagtaa aactaatgtg tgtatcagta ttaattgtat tttgctaaaa tttttgtact
     1921 tcatggtatc tcccttatac atcataaatt ttattgtgca atgccattcc caattgtggg
     1981 ttgttgtcag catatgcaat attcaatttt agacttaacc tctgtttaca agcgttacgc
     2041 tatctcctgc aatagagtgt gaaaaaagtt taaggcaact caaaaactgt gagcgccatg
     2101 tgcacctata gtgatatttg cctgtacaat aatttcatgg tttggtttat tagtaccact
     2161 ttctgaatat aaacttttat cgtaattata ctgcaacctg aagcgtgaaa attcagatgg
     2221 attatattca atcatagaac tgtatcgtgg aagattattg gtaaggtcat ggtttgtact
     2281 gacatcattt tgagccagca gatcgtatcg caaaccaatg cgccatagtt gattcaaccg
     2341 ggcaaccact tgagtatata atccagactg tttggcgtca aaatttgatt tgctatttat
     2401 actgccatcg taactataca aatcgccatc ctgattgcga tagatatatt ctgtttgtaa
     2461 atatatatat tgatacgaat taaaatcata tcgtattgtt atatcaccaa tagccatata
     2521 cgtttttcca taaaaaccat gatcaccggc aacattagta tcattgccaa tgccatgatc
     2581 aattcgcgag gtaccgtata ttcctgatac tcctgccagc acggtgtagt tacctatatc
     2641 aaatgaagat ttaacaaagg cggtatatac attgggttta ttagcatcat caatcgtcgt
     2701 tatgttttca acggtaaaac cgttggttcc aaaacttgtt tcgttttcac cccgtaaagc
     2761 ttctgcacct atcagcatat aaaaaggtaa tggtactatc cagtttaact gtatgccaat
     2821 ttcattaagc tgtgaaccaa agaaagcctt atagacaagc ggtaaatctg caaaatccca
     2881 gtcatgggca tgcttatcgt ttagtctgcc aatggaactt aaaaatttac ctccttttat
     2941 tttaaaacca aatggtagtt gctgtgtggt aaaataggcc tcctcaattt cagctccttc
     3001 tgttgatgca tgcaaaacag caaacatatc aaagaatgga tcaacctgtg agtacaatga
     3061 aatctctaca taattaaggt taaaaccatt gttttgattt aattcatctt catgattttc
     3121 atggttggat ggtttactgt gtgcatcctt aaaaccgggt atttcatagt gtgtaacgtc
     3181 atcaagtgaa cgctgaacat acgttacatc tacaatagca gctatatccg gtacaaattt
     3241 agcctgcgaa aaaggatccg atgtttgcca atctgccatt gtttgtggat atcctttttg
     3301 taacaataga gatataaaaa acagcatata tactatttgc ataaattgct tcatttagta
     3361 tcctcctcag gaaataaaat actgtaccac tctgagggac tccgtcccca agaatctatg
     3421 gatttataaa ggagattctt tgctattgct cagaattatg gtactgtttc gtttttattg
     3481 tttattaggt ggccgcactg cagccatgga taactcccct aaatggtata gctatgtcat
     3541 gttaaaactt gctgtaaaat ataatcagca atacagtgca aaaattttag ttgagatttg
     3601 tgataatggt tatatattaa ctattgacag gaggggagcg ggaggctaca ggattggtaa
     3661 tcagtagaat ggggaagttt ttgataatac aatgtacact actatataat gaaagatact
     3721 caaatgctgt ttgatcaata gcgtcaaatg aatcaagctg ctggtatatg caaaccgggc
     3781 aattatgatg agcggaggta tgaaaatgga cagtactgat aaagttactg tatgataaca
     3841 aaagagtaat aaaaagaata aatggatagc ttaaaatttt cattttcatg ctattaacta
     3901 tattacaggg cgatactatt ttcaaaaaaa ataatacttc agcttatcca tcaggcaaaa
     3961 tgccacgctg aattgccatt tctttcatct gctcaccact tgtttttggt ggtatacgtt
     4021 ttttgtcttt tagtacaagg tgtattgcat cagcatggca ggctgaaacg cataatccgc
     4081 atccaataca tctatcctta ttaacatgtg caaaatcgtt catttcaatt gcttccatct
     4141 ggcatatatc agcacagctt ccgcatgaag tacagttgtc atttacttct gcaaaatagt
     4201 ttgaaaacac catctcggca ggtt
//
LOCUS       DAOSZP010000133         4194 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_17207_length_4194_cov_3.810582, whole genome shotgun sequence.
ACCESSION   DAOSZP010000133 DAOSZP010000000
VERSION     DAOSZP010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4194
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17207_length_4194_cov_3.810582"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..393)
                     /locus_tag="PK348_04085"
     CDS             complement(<1..393)
                     /locus_tag="PK348_04085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65427.1"
                     /translation="MLLSPDDSILFFKLYFSLLLYANSNFKILENIYSANDIRQSDPN
                     NIIKIREQLYNHPELFDLFIHENPFKFSNNELSIIRSWKQFIKKKFIIFRYAKNYTIF
                     IDDTSDDPKAYGVLAITSPLHEMVGSQLP"
     gene            647..1711
                     /locus_tag="PK348_04090"
     CDS             647..1711
                     /locus_tag="PK348_04090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HXK65428.1"
                     /translation="MKRLCTIKAGKRAYRIIKDEGLKKERIQCVLGAAGGPKWLILYG
                     LDKLVYSHFLQKRKTPVHLIGSSIATWRFAALAQKDGAKALDTFKERYMAQKYSLNPD
                     REEITAETVAIMETYLPDSRVKEIMQHPVIRLCIITARSKHLLTNERKLPLMAGLALA
                     ALSNAVSRKLLALHFDRYVFYDERQFPSFIQWNNFSTQYIPLDASNVKKAIMASGAIP
                     LVMQGVPYSYNGTATMLRDGGLVDYQMDLPLTLDGGIALYPHYSEHIVPGWFDKALPH
                     RKPQNTLDDICMVSPSPDALDLLPYKRIPDRHDFYTFAGNDAERLSFWEKALEVGQKM
                     ADEFAELVESGKIKDRVVLL"
     gene            1899..3596
                     /locus_tag="PK348_04095"
     CDS             1899..3596
                     /locus_tag="PK348_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013258323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HXK65429.1"
                     /translation="MILLNPKKYNVKFPDKESEDIMKKTIQFFENKGLKKVLDDDHNC
                     VWYDDFLQFQKENQIFAKLFTPKGYGDKDSHWNTWRIVNFAEILGFYGLAYWYTFQVS
                     QLGLGPIWIGKNEEVKQKAAKLLKEGAIFAFGLSEKEHGADIYSSDMMLYPNGDGTYR
                     ANGDKYYIGNANKAGLVSTFGKIADTGDYVFFVVDSQHPKFECIKNVIHSQSYVAEYA
                     LHDYPITEADILDKGRDAWDASLNTINICKFNLGWGSIGIATHCFYEALNHAAHRNLY
                     GKYVTEFPHIKQFFVDAYTRLVAMKLFALRATDYMRSANENDKRYLLYNPMVKMKVTT
                     QGEEVINLLWDIIAAKGFEKDTYFHMAARDIRALPKLEGTVHVNMALIIKFMFNYFFN
                     PKEFPEVPQRLDATNDDFLFNQGPTKGLGKIQFHDYNIAYNSVNLPNVEIFKKQINAF
                     KDFMMGATPDQKQAEDIDFLLSIGELFTLVAYGQLILENKKFYNVEDDLIDQIFDFMI
                     RDFSKFALQIYSKTSSTAKQQELCLKMIQKPVVDHDRYNRVLEKYVYSLVDEYHMNE"
     gene            complement(4004..>4194)
                     /locus_tag="PK348_04100"
     CDS             complement(4004..>4194)
                     /locus_tag="PK348_04100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65430.1"
                     /translation="ILQNILVKGKNTIDRTLPFLTGTTERANQIIIDILQVRFYYQYN
                     YYGIFFPFGFKYKTVCHI"
BASE COUNT         1318 a          727 c          834 g         1315 t
ORIGIN      
        1 aggtaattgt gatccaacca tttcatgtaa aggtgaagtt attgctaaca cgccataggc
       61 tttgggatca tcgctggtat catcaataaa aatagtatag ttttttgcat aacgaaaaat
      121 tataaacttt tttttaataa attgtttcca tgatctaatt atgcttaatt cattattaga
      181 aaatttaaat ggattttcat gaatgaataa atcaaataat tcaggatgat tatataattg
      241 ttccctaatc ttgattatgt tgttaggatc ggattgacgt atatcattag ctgaatatat
      301 gttttccaga attttaaaat tgctgttagc ataaagcagc aatgaaaagt acagcttaaa
      361 aaatagtata ctgtcgtctg gtgataaaag cattgtttct ccacatattc tttttaaaga
      421 tattggaaga ttattattgc atataatatt ggaattttta tatatgtgtt tttcaattat
      481 ttttttatat tttttagaat aaaatggttt tctattagta cttatccaaa ataatcaaat
      541 tatatagtat acattcaagt acaaaataaa ccaaaaaatc cttgctttta tccccatacc
      601 ataacactgt tattcaaaaa tactgttcca ataatctgag gatactatga aacgtttgtg
      661 tacaataaaa gcaggaaaac gggcatatcg tatcattaaa gatgaaggcc tgaaaaaaga
      721 aagaattcag tgtgtacttg gtgctgctgg tggtcccaag tggctgattc tatacgggct
      781 tgataagctt gtgtattcgc actttttgca aaaacgaaaa acgccagtgc atttaattgg
      841 ttcatccatt gcaacatggc gctttgctgc gttggcacag aaggatggtg caaaggcact
      901 ggatacattt aaagagcgct acatggcgca gaaatattcg cttaatcctg acagggaaga
      961 gataacagcc gaaactgttg ccattatgga aacctacctg cctgatagca gagtaaaaga
     1021 aattatgcag catcctgtta tacgtctgtg tatcatcaca gcgcgatcaa aacatctact
     1081 tacaaatgaa cgtaagcttc cgctcatggc gggtttagct ttagcggcgc tttccaatgc
     1141 tgtaagcaga aagcttttag cattacattt tgaccgctat gtgttttatg atgagcgaca
     1201 attcccgtca tttattcaat ggaataattt cagcacgcag tatatcccgc ttgatgcatc
     1261 aaatgtaaaa aaggccatca tggcttcagg tgccatccca ctggttatgc aaggggttcc
     1321 gtattcctac aacggcactg ccaccatgct acgcgatggc ggacttgttg attatcaaat
     1381 ggacctgcca cttacacttg atggtggcat tgcgctgtat ccacattact ctgagcatat
     1441 tgtgccggga tggtttgata aggcgttgcc gcatcgcaaa ccacaaaata ccttagatga
     1501 tatatgtatg gtaagcccgt cgcctgatgc gcttgattta cttccctaca aaagaattcc
     1561 tgacaggcat gatttttaca cgtttgcagg caatgacgct gaaaggttat cattctggga
     1621 aaaagcactt gaggtgggac aaaagatggc ggatgaattt gcagaattgg tggaaagcgg
     1681 gaagattaaa gacagggtgg ttttattgta gtgatgcatt ggaattaatg atgtgccaat
     1741 tgaggcgtat gtacggcggg ggcacgccca taattcacat tgacaaatat aaaaaatagt
     1801 tgattaaaaa aatacataac actgtaattt tttctttaca aatcagtaca gacatgatat
     1861 actcaaattt gttactataa tttgaatagg ggtgggatat gattctttta aatccaaaaa
     1921 agtacaatgt caaatttccc gacaaagagt cggaagacat tatgaaaaaa accatacagt
     1981 tttttgaaaa caaaggatta aaaaaggttc tggatgatga tcacaactgt gtgtggtacg
     2041 atgacttttt gcagtttcag aaggaaaatc agatttttgc taaactcttt acaccaaaag
     2101 ggtatggcga taaggacagc cactggaata cctggcgcat tgtcaacttt gctgaaattt
     2161 taggatttta tgggcttgct tactggtata cattccaggt ttcgcagctt ggtcttgggc
     2221 caatatggat tggcaaaaac gaggaagtta agcagaaagc tgccaaactt ttaaaagagg
     2281 gtgcaatctt tgcctttggt ttgtccgaaa aggaacatgg cgctgacatc tattcaagtg
     2341 atatgatgct gtatcccaat ggcgatggta cttaccgtgc aaatggcgac aaatactaca
     2401 ttggcaatgc caacaaagcc gggctggtgt caacatttgg caaaattgct gatacgggcg
     2461 actatgtgtt ctttgtggta gattcacagc atccaaaatt tgaatgtatt aaaaacgtta
     2521 ttcacagcca gtcatacgtt gctgagtatg cgctgcacga ttatcctatt accgaagctg
     2581 atatccttga taaaggcagg gatgcctggg atgcatcgct caatactatt aatatctgta
     2641 aattcaattt aggctggggt tcaattggta ttgctaccca ttgtttttat gaagcgttaa
     2701 atcacgctgc acaccgtaac ctgtacggca aatacgttac tgaatttccg cacatcaagc
     2761 agttctttgt tgatgcatac acacggcttg tggcaatgaa attgtttgct ctgcgtgcaa
     2821 ctgactatat gcgaagcgct aatgaaaatg acaagcggta tttactctac aatccaatgg
     2881 ttaaaatgaa agttaccaca cagggtgaag aggttattaa cttgctatgg gatatcattg
     2941 ctgctaaagg ttttgaaaag gatacatact tccacatggc tgctcgcgac atcagggcat
     3001 tgccaaaatt agaaggaaca gttcatgtta acatggcgct gattatcaag ttcatgttca
     3061 actacttctt taatcctaag gaattccctg aagtaccgca gagattagat gctaccaacg
     3121 atgatttcct attcaatcag ggaccaacaa agggattggg caagattcag ttccacgatt
     3181 acaacattgc ctataacagc gttaaccttc ccaacgttga gattttcaag aagcagataa
     3241 acgcattcaa ggatttcatg atgggtgcaa cccctgacca gaaacaggct gaagatattg
     3301 actttttatt gtcaattggt gagttgttta ccctggttgc gtatggtcag cttattttag
     3361 aaaacaagaa gttctacaat gttgaagatg atttgattga tcagattttt gatttcatga
     3421 tcagggattt ttcaaaattt gcactgcaga tttactcaaa gacaagcagt actgcaaagc
     3481 agcaggagct ttgcttaaag atgatacaga agcctgtggt agaccatgat aggtataacc
     3541 gcgtgcttga aaaatatgtg tattcgctgg ttgatgaata tcatatgaat gagtaaagat
     3601 tataacgggg atgcgatagt tatggactat ctgcatcctc gtttttatat ccaattgact
     3661 ctaaatcagc caaatcctga tatctaccta atgccttttt gtttttaata aactgctgtt
     3721 tcccaataaa aagtactttt acatcaccat aatttcctgt aactgcatta ttatagactt
     3781 catcccagct aacaccagta atagaagtga ttaaatcaat tcttaaagga ggatatcttt
     3841 ataactcttt gtaatcttga tgtatttcca tacatttgcc tgcgcagtat ttcaacggca
     3901 ctgatgcgtt cttcaggggt tttggttatc caccaattgt aattatcagt gggtttacaa
     3961 tcttttatat gtttctttat gatatgtggt tgtattgagt gattcatata tggcatactg
     4021 ttttatactt aaatccaaaa ggaaagaata taccgtaata gttatattga taataaaaac
     4081 gaacttgcag tatgtcaata ataatttgat tagctctttc agttgtacca gttaaaaaag
     4141 gtagtgtcct gtcaattgtg tttttaccct tcactaaaat attttgcaaa ataa
//
LOCUS       DAOSZP010000134         4170 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_17332_length_4170_cov_3.088700, whole genome shotgun sequence.
ACCESSION   DAOSZP010000134 DAOSZP010000000
VERSION     DAOSZP010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4170
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17332_length_4170_cov_3.088700"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..1316)
                     /locus_tag="PK348_04105"
                     /pseudo
     CDS             complement(1..1316)
                     /locus_tag="PK348_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012860042.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
     gene            1505..2800
                     /gene="purB"
                     /locus_tag="PK348_04110"
     CDS             1505..2800
                     /gene="purB"
                     /locus_tag="PK348_04110"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HXK65431.1"
                     /translation="MIDRYSRKQIASIWSLENKFKIWLDIEIAACEANAQLGVIPQED
                     LAIIKEKANFNVSRIEEIENQVHHDVIAFLTCVAEYVGPSSRYIHYGLTSSDIVDTAL
                     SIQMKQAGEIILNDIDQLLEVLKKLALKYKHTPCMGRSHGVHAEPTTFGTKMGLYYHE
                     FLRNRKRMQDAIDEISYGKLSGAVGTFSNIDPRVEEIVCKKLGLKPDPISTQIIQRDR
                     HAHYMSAVAICAGTLDKLATEIRHLQRTEVREVEEPFQKGQKGSSAMPHKRNPILSER
                     VSGLSRVIKANLMVALDNMTLWHERDISHSSAERVILPDSTIALDYLLSKMIFILENL
                     HVYPDAMMKTIEKTGGLFYSQGLLLKLVEKGYTREEAYQIVQDNAMRVWNAGGSLKEL
                     SLNDGRITAKLSHDEIEEIFKLENFLHNIDYVFKKAGLE"
     gene            complement(2845..>4170)
                     /locus_tag="PK348_04115"
     CDS             complement(2845..>4170)
                     /locus_tag="PK348_04115"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HXK65432.1"
                     /translation="LYTDTLKFIKNIKTMKYPKIEGVFDGNQAVLYGYNVPQSNRTII
                     AAFNEKILYKTVYRSLFFIILGMIVVNLIIRYLINKQLMKLSKAIDIAMPALQKATKG
                     DLSGEITLIKSRDIMEDFIRSFIGFREVIIEFIQKSNELSEKVLISSEALAFSGKSIK
                     EEASQQAQNIEQVTSIIAEVSSSFYTIASDANKQSDILKNLEDIVNNLNAAMNILNND
                     AQQVIVAIRGVEKSAHDSENLVTSTAESSRQIASFFENIMNVIGLISDIAEQVNLLSL
                     NASIEAARAGEHGRGFAVVAEEISRLADRTAQNVKEISKIINEGNTAMNSNMSMMDTM
                     RKVLMNIMQDIERSVTLITGFMETINQRVQDFAKIHEGITSASEFSKGLSLSMAELSD
                     NTKKIVQSIENVNAVAGQFVALSENLTNTSSELESMAAELKKVIEHFKI"
BASE COUNT         1342 a          811 c          722 g         1295 t
ORIGIN      
        1 agaaaagatt tgtttcattt tttattacaa atgaaagacc aagcaacgct atcaaatttg
       61 ggaatgccat gagcccattc attatatcag ttaacgtcca tactgtttct aattttccga
      121 tagctcctat aaaaacaact atcacccata atactctata tggctttatt gccttttcac
      181 ctgcaatgta ttcaaccgat ttttcaccgt agtatgccca tcctaaaata gtggaatacg
      241 caaaaaatat aaggctgaga gaaacaacaa taccacccca ggttcctggt taagcccaaa
      301 ctaaaagcat gttcggtcaa tgctgcggct gtttttccac cttcccatgc accagttacc
      361 agtataacca gacccgtaaa cgtacacaca acaatggtat caataaaagt ctgcgtcatt
      421 gaaaccagag cctgccttac cggttcatca gtctttgctg ctgcagcagc tattggtgag
      481 ctacccaaac cagattcatt agataataaa cctctggata cgcccatctg catagccatt
      541 ttaacagtag caccagcaaa tccaccagca gcagctacag gcgtaaacgc atgcacaaat
      601 ataagttcaa aagcatgtag aatattaacc cggtaaatta ttaatattat tgttccggct
      661 attatataaa ataaaatcat tattggaact atcgccgaag ctgcccgagc aatacttttg
      721 atgccaccca taataacaag aaacgttaaa ataaaaagta caataccggt aatccagtga
      781 tcgatgttaa aggttgcttt catagcttca gcaacggagt ttgattgtat catgttgcct
      841 gtaccaaatg cagcacatgc agcaaataca gcaaatatca tggcaagcca gttattcttt
      901 aatccttttt ctatataata cattgggcca cctgacatgg ttcccagtgc atcaacttca
      961 cgatatttga cggcaagaac tgcttcacta tactttgtgg ccattccaaa taaccctgta
     1021 agccacatcc agaacattgc acctggccca cctatagcaa tggctgatgc aacaccagca
     1081 atatttccag taccaacagt agcagccagt gctgtcatta aagcctgaaa atgtgagata
     1141 tcaccaggat gtgcgccaaa ttcctttcgt ttaattagtg ccaggtataa agaagaaaac
     1201 agtgtcctga attgtaatcc tttgagcaat atagtaagat atattcctgt accaagcagt
     1261 aaaatgatca gtggagttcc ccacacaaaa tcgcgtattt gatttaaata ttccatagta
     1321 taccttgata aaaaatttgt atcgttttac attgatgcta tattgttacc ctatccgtca
     1381 agcagtaatt ttactaacaa tccaaattat cttttttact tgattatttt ccttattgaa
     1441 ggaaaatttg tcttttcctt tatttattat taactatata ttgatattga agtatgaggt
     1501 ttgtatgatt gatagatatt cacgcaaaca aattgccagt atatggtcac tggaaaataa
     1561 atttaaaata tggcttgata ttgagatagc cgcctgcgaa gcaaatgcac agttaggcgt
     1621 tataccacaa gaggatttag caataatcaa agaaaaagct aattttaatg tttcgcgcat
     1681 tgaagagata gaaaatcagg tacatcatga tgtgatagca tttttaacct gtgttgccga
     1741 atatgtagga ccatcaagca gatacattca ttatggcctc acatcaagtg atatagttga
     1801 tactgcactc tcaattcaga tgaaacaggc tggtgaaata atacttaatg atatcgacca
     1861 gcttctggaa gtactgaaaa aattagcact gaaatacaaa cataccccat gtatggggcg
     1921 atcacatggc gttcatgcag aaccaacgac atttggcacc aagatgggat tgtattatca
     1981 tgaattttta cgtaatcgta aacgaatgca ggatgctatc gacgaaatat catatggaaa
     2041 actttcaggt gctgttggca cgttcagcaa tattgatccg cgtgttgaag agatagtttg
     2101 taaaaaatta ggcctaaaac ctgatccaat atcaacgcaa ataattcaac gagatagaca
     2161 cgcacattat atgtcagctg tagctatttg cgcaggaaca ctggacaaat tggcaaccga
     2221 aatacgccac ctgcagcgga ctgaagttcg agaagttgaa gaaccttttc agaaaggtca
     2281 aaaagggtca tcggcaatgc ctcataaacg aaatcccatc ttgtccgaga gggtatctgg
     2341 gttatcacgg gtaataaagg caaaccttat ggttgcgttg gataatatga cgttgtggca
     2401 tgaacgtgat atatcacatt catcagccga gcgtgttata cttcctgaca gcactattgc
     2461 gcttgattat ctgctttcaa agatgatatt tatccttgaa aaccttcatg tgtatcctga
     2521 tgccatgatg aaaactattg aaaaaacagg tgggttgttt tattcacagg gtttattact
     2581 taaactggta gaaaagggct atacccgtga agaggcatac cagatagttc aggataatgc
     2641 aatgagagtg tggaatgcag gaggctcttt aaaggaacta tcactcaatg atggaaggat
     2701 aactgcaaaa ctttcgcacg atgaaataga agaaatattc aaactggaaa atttcttaca
     2761 taatattgat tatgtattta aaaaagcagg tttagaataa aacatcaaac atttcagact
     2821 gaaacggcgg aagtagatgt ttcatcatat cttaaaatgc tcaataactt ttttaagctc
     2881 tgcagccata gattcaagtt ctgatgaagt gtttgtcaga ttttcagata gagcaacaaa
     2941 ctggccagca actgcattaa cgttttcaat ggattggact atctttttgg tattatcact
     3001 caattcagcc attgatagcg atagtccttt tgaaaactca ctggcactgg taataccttc
     3061 atgaatcttt gcaaagtcct gaaccctttg attaattgtt tccataaaac ctgttattaa
     3121 tgttacactg cgttcaatat cctgcataat gttcatcaat actttacgca tggtgtccat
     3181 catgctcatg ttgctattca ttgccgtgtt gccttcatta attattttag aaatttcctt
     3241 aacattttgt gctgtcctgt ctgcaagtct tgaaatttct tctgcaacca ctgcaaatcc
     3301 tctgccatgt tcaccagccc ttgctgcctc aattgatgca ttgagtgaca aaaggttaac
     3361 ctgttcagca atatcagata ttaaacctat aacattcata atattttcaa agaaagaagc
     3421 tatttgcctg gatgattctg ccgtactagt tacaagattt tcactgtcat gagcgctttt
     3481 ttcaactcct cgtattgcga caatgacctg ctgtgcatcg ttatttaata tattcattgc
     3541 ggcattaaga ttattgacaa tatcttcaag atttttcagg atatcacttt gcttattggc
     3601 atcagaagca attgtataga aggaactgga tacttcggct ataattgaag taacctgttc
     3661 aatattttgt gcctgttgtg aagcctcttc ttttattgat tttccagaga atgctaaagc
     3721 ttcggatgaa atgagtacct tctcgctgag ttcgtttgat ttttgtataa attcaataat
     3781 tacttccctg aaaccaataa aacttcgtat aaaatcttcc atgatgtcac gggattttat
     3841 taatgtaatt tcccctgaca gatcgccttt ggttgccttt tgcaaagcag gcatagctat
     3901 atcgatagct tttgaaagtt tcatcagttg tttattaata agatatctga taattaaatt
     3961 tacaactatc atcccaagta ttataaaaaa taaactcctg tatacagttt tatacaatat
     4021 cttttcatta aaggcagcaa ttatagtacg attgctctga ggcacattat aaccatataa
     4081 cacagcttga ttgccatcaa atacaccttc tatttttgga tacttcattg tttttatatt
     4141 ttttataaat ttcagagtat cagtatataa
//
LOCUS       DAOSZP010000135         4122 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_17594_length_4122_cov_3.171625, whole genome shotgun sequence.
ACCESSION   DAOSZP010000135 DAOSZP010000000
VERSION     DAOSZP010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4122
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17594_length_4122_cov_3.171625"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..422
                     /locus_tag="PK348_04120"
     CDS             <1..422
                     /locus_tag="PK348_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005298674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HXK65433.1"
                     /translation="PNSVVGSTHYRHGERVHIILSLQKRNYGLICECEMVTLGEVEYA
                     IKNLKVKDLVDLRRRTRIGMGPCQGELCAYRAAGLFTEFGEFTPDTSTKLLKDFLEER
                     FKGMKPVLWGDGLREFEFTYWIYQGLFGLGLVEEEEQ"
     gene            419..1714
                     /gene="glpB"
                     /locus_tag="PK348_04125"
     CDS             419..1714
                     /gene="glpB"
                     /locus_tag="PK348_04125"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR03378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HXK65434.1"
                     /translation="MKFDCIIIGGGISGLTAGIRCVKEGLHTAIISSGMSALHFSSGS
                     IDVLGYINNKVVYKPFEALDTLTGTIAQHPYTKCGKPLIIEALHFFKQECETQNVYLF
                     DNENLNHFHVTALGTLKPTYFSQRSVFNEDVYNRFKSMKKIVIVNFEGYRDFHPQLAA
                     AQLSKHALFANCEIIAKSIDMPQLQDLTRHPHEFRSIDIARIFEDEENVYKIADAIRL
                     AAEGADFVVIPAFMGIHNFNQIHNLLQSRTRLLIYEVPTLPPSILGLRIDNALKSRFA
                     QLGGVYIAGDKVVRAEINNDVVQAVYTANHPENPLEAKFYILSTGSFFSGGMISEFNN
                     IYEPIFKLKLHYEPERNKWYSPQFFYKNSHPFLEYGVVTDENLRPYDSNNNIIQNVYC
                     SGAILAHYNPIREGCGSGVAISTAYKAANQIISLYKLMR"
     gene            1719..2915
                     /gene="glpC"
                     /locus_tag="PK348_04130"
     CDS             1719..2915
                     /gene="glpC"
                     /locus_tag="PK348_04130"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005298671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit GlpC"
                     /protein_id="HXK65435.1"
                     /translation="MIKLENISFDHCIKCTVCTIYCPVARVTHLYPGPKQSGPDTERL
                     RIKNPQLLDISLKYCSNCKRCEIACPSDVKIADIIQTAKWRYSKKEFRIRDFLLSRTD
                     LMGTFGTIFSPIVNFFTHLGIVKYFLDLIFQIPKGRTFPNYASMTFRKWFKEAKKYQN
                     KYLEKVVYFHGCYVNYNYPQLGKDVVKVLNALGKGVIITKEKCCGVPLIANGYIKKAQ
                     SHGRHNIRVLSRALPNEQTRIVSASSSCSLALKHEYPNLLELDNSPIANNIAYITQYV
                     LEEFEKGNIPNLKPLKYTVAYHSPCHLERMGNVAFTIELLRKIPGLTLKILHSECCGI
                     SGTYGFKNEYYAISQAVGSELFKIIEEAKPDFVVTDCETCKWQIEMNTQYTVIHPISL
                     LAEAIE"
     gene            complement(2925..3479)
                     /locus_tag="PK348_04135"
     CDS             complement(2925..3479)
                     /locus_tag="PK348_04135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65436.1"
                     /translation="MIKRYVALFVCLLFPLYSEAQDCVSISFNTLRMLAESRIDPCPI
                     CQKKLQRQALGQITKDIFPGKKIMFNSSCSLIKTQQTKDNELMLNTDYDSKIMINGIE
                     TNLPYIILKFYTHENHIVGIDEKDYTSREIQNLYKNLMYNKPVEAEVIVISYKYSDFA
                     SSIYLPERNTLIIHCKVIRLQQIQ"
     gene            complement(3487..>4122)
                     /locus_tag="PK348_04140"
     CDS             complement(3487..>4122)
                     /locus_tag="PK348_04140"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXK65437.1"
                     /translation="GWFISSQTARFTVCYFHGNAGNIYDRIELLKLLHSIPLSIFIID
                     YRGYGKSSGKPSEKGLYEDAMAAWEYLTDVKKISPKDIILFGRSLGGPVALYCASRTN
                     PAGIIIDSSFTSIKSMVQYHSSGCMSLFFKEKYPAIDYIQKIAIPVLILHSRDDETAP
                     FSMGQELYRQCPSNKKYFVELRGGHNNNFLVDAPLYRNSIKEFVLSLTSEE"
BASE COUNT         1307 a          721 c          799 g         1295 t
ORIGIN      
        1 ttcccaattc agtagttggc tccacgcatt accgccacgg tgaacgtgtt cacattatcc
       61 tgagtttgca gaagcgtaat tatgggctta tctgcgaatg tgaaatggtt acactgggtg
      121 aagttgagta tgccattaaa aatctcaaag taaaagacct tgtagattta cgacgaagaa
      181 cacgtattgg catggggcca tgccagggcg agttatgtgc ttatcgtgct gcaggacttt
      241 tcactgaatt tggtgaattt acgccggata catcaacaaa acttttaaaa gatttccttg
      301 aagaacgttt taaagggatg aaaccggtac tgtggggtga tggattacgg gaatttgaat
      361 ttacctactg gatttatcag gggttatttg gcctgggact tgttgaggaa gaagaacaat
      421 gaaatttgat tgtataataa ttggtggtgg aatttccgga ttaactgcag gtatacggtg
      481 tgttaaggaa gggctgcaca ctgcaatcat ttcatcggga atgagtgcat tacatttctc
      541 ttccggatct attgatgtat tgggatatat aaacaataag gtggtgtata agccttttga
      601 ggcattggat accttaacag gaactatcgc ccagcatccg tatacaaaat gcggcaagcc
      661 attaattatt gaagcattgc atttttttaa acaggaatgt gaaactcaaa atgtatatct
      721 ttttgataat gaaaacctca atcatttcca tgttactgca ctggggacgc ttaagccaac
      781 gtacttttct caacgaagcg tgttcaatga ggacgtgtat aaccgtttta agagcatgaa
      841 aaaaattgtc attgtaaatt ttgaaggcta tcgtgatttt catcctcagt tagctgcagc
      901 acagctttca aagcatgcgt tatttgcaaa ctgtgaaatt attgctaaaa gtattgatat
      961 gccccagctt caggatttaa ccaggcatcc tcatgaattc agatccattg acattgcccg
     1021 tatttttgaa gatgaagaaa atgtatataa aattgctgat gctatacgac tggcagccga
     1081 aggtgctgat tttgtggtta tcccagcttt catggggatt cataacttca atcagataca
     1141 caatttgttg caaagtcgca ccagattact catttatgaa gtgccaacgc ttccgccatc
     1201 aattcttgga ttgagaattg acaatgcgct gaaatcacgg tttgcacagc tgggcggtgt
     1261 gtatattgct ggcgacaaag ttgtgcgtgc agaaattaat aatgatgtgg tgcaggcagt
     1321 atatactgcc aaccatccag aaaatccgct ggaagcaaaa ttctatattc tatcaacggg
     1381 cagttttttt agtgggggga tgataagtga attcaataat atctatgagc caatatttaa
     1441 actgaaactt cattatgagc ctgaacgcaa taaatggtat agcccgcaat tcttttacaa
     1501 aaattctcat cctttcttag agtatggtgt tgttaccgat gaaaatttac ggccgtatga
     1561 tagcaacaac aatattatcc agaatgtata ttgcagtggg gcaatacttg cacattataa
     1621 tccaatcagg gaaggctgtg gaagtggtgt ggcgataagc accgcataca aggctgcaaa
     1681 tcagattata tctttatata agcttatgag atagcaccat gataaagtta gaaaacataa
     1741 gttttgacca ttgtataaaa tgtacggtat gtaccattta ctgcccggtt gcacgagtta
     1801 cacatttgta tcccggtccc aagcagtcag ggcctgatac tgaacggctt cgcataaaaa
     1861 acccacagct tcttgatata tcattaaagt actgttctaa ctgtaaacgg tgtgaaattg
     1921 catgcccatc tgatgtaaag atagcagaca ttatccagac agcaaaatgg cgctattcaa
     1981 aaaaagaatt caggatacgg gattttttac tcagtcgtac tgatttaatg ggaacgtttg
     2041 ggacaatatt ttcacccata gtcaattttt ttacccattt aggaattgtt aagtactttc
     2101 ttgacctgat tttccagatt cctaaaggca gaacatttcc aaactatgca tccatgacat
     2161 ttagaaaatg gtttaaagaa gcaaaaaaat atcagaataa atatcttgaa aaggtagtat
     2221 attttcatgg gtgttacgtt aattacaatt atcctcaatt aggaaaggat gtagtaaaag
     2281 tactcaatgc attgggcaag ggtgttatta ttaccaaaga aaaatgctgt ggtgttcctt
     2341 taatagcaaa tggatatata aaaaaagcac aatcgcatgg caggcataat atacgggtgt
     2401 tatccagagc actgcccaat gaacaaacca ggattgtttc agcatcatca agctgttcgt
     2461 tagcattgaa acatgaatat cccaatttgc ttgaacttga taacagccct attgcaaaca
     2521 acatagcata tattacgcaa tatgttcttg aggaatttga aaaaggcaac atccccaatt
     2581 taaaacctct taaatatact gtggcatacc attcaccctg ccatttagaa cgcatgggca
     2641 atgttgcatt tacaattgaa ctgttgcgta agatacccgg attaaccctg aagatactgc
     2701 attctgaatg ctgtggtata tccggtacat atggctttaa aaatgaatat tatgccattt
     2761 ctcaggcagt gggaagcgaa ctctttaaaa taattgaaga ggcaaaacca gattttgttg
     2821 taaccgattg tgaaacctgt aaatggcaga tagagatgaa tactcagtat acagtaatac
     2881 atcccatttc attgctggca gaggcaattg agtaaataaa gaatttattg tatctgttgc
     2941 aaacggataa ctttacaatg gataattaat gtatttcgtt cgggcaaata tattgaagat
     3001 gcaaaatcac tgtatttata ggaaatgaca ataacttctg cttcaacagg tttattatac
     3061 attagatttt tatacaaatt ctgaatttcc cttgaagtat aatccttttc atcaattcca
     3121 acaatgtggt tttcatgtgt gtaaaacttt aaaataatat atggcagatt agtttctatt
     3181 ccattaatca taatttttga atcatagtct gtgttcagca ttaattcgtt atcttttgtt
     3241 tgctgagtct ttatcaggct gcatgatgaa ttaaacatta tttttttacc tgggaatata
     3301 tcttttgtaa tttgtcccag tgcttgcctt tgaagtttct tctgacatat tgggcatggg
     3361 tcaatgcggc tttctgcaag cattcgcaat gtattaaaag atatggacac acaatcctgt
     3421 gcttcagaat atagtggaaa caacaagcat acaaatagag ctacatacct ttttatcata
     3481 gtgaaatcac tcctcactgg ttaatgataa aacaaactct tttatactgt tacggtatag
     3541 tggtgcatca acaagaaaat tattattatg tccacctcgc aactctacaa aatatttttt
     3601 gtttgatgga cattgtctgt aaagttcctg tcccattgaa aatggtgcag tttcatcatc
     3661 cctgctatga agtatcagaa caggtatagc tattttttgt atgtaatcaa tagctggata
     3721 tttttctttg aaaaacagtg acatgcatcc tgagctgtga tattgcacca tggatttaat
     3781 actggtaaaa cttgaatcaa ttataatgcc tgcagggttt gttcttgatg cacagtacaa
     3841 agctacaggt cccccaagtg aacgcccaaa caaaataata tcttttggtg atattttttt
     3901 aacatcagtc aaatattccc atgcagccat tgcatcttca tacaatcctt tttcactggg
     3961 tttgccactg cttttaccat atccgcggta atctattatg aaaatagaaa gcggaattga
     4021 atgtaaaagt tttaatagtt caattctatc atatatgttg cccgcattac cgtgaaagta
     4081 acatacagta aaacgagctg tttgtgaaga aatgaaccag cc
//
LOCUS       DAOSZP010000136         4115 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_17628_length_4115_cov_3.672660, whole genome shotgun sequence.
ACCESSION   DAOSZP010000136 DAOSZP010000000
VERSION     DAOSZP010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4115
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17628_length_4115_cov_3.672660"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(757..1719)
                     /locus_tag="PK348_04145"
     CDS             complement(757..1719)
                     /locus_tag="PK348_04145"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HXK65438.1"
                     /translation="MKKNVLISIAVIAVLAGYLIINSLIPKNVVTLPTIKSPVTELVL
                     KKGEAIILIKQDGDSWKVNEFEGDKNIIANMVNKIKDLAITDLISDKPHYERYELDND
                     HAIRVQAKSNGQIVRDVYIGKKGSTYRHTYVRFEGDPKVYLAEDTLDSYFNKTVDELR
                     NKDIVQLDIAKIDTVKLWYKNTSLTLQKKVVEKKNEEGNAQKEKQEKLKEIEKEEQWI
                     CKEQPAVILDNNKVKEMLYTFDPLKAYSFPDVNAKTLTGKIASITVSVKGEESQKPED
                     ITLVIHKEYENNRYICTSSKSQYVFTLDEWRAKRIFKTLQDLKK"
     gene            complement(1746..3896)
                     /locus_tag="PK348_04150"
     CDS             complement(1746..3896)
                     /locus_tag="PK348_04150"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HXK65439.1"
                     /translation="MNKRNIIDMLQNLKSNITIIYTKVIQNIKEQTKSGDTETQKSYS
                     QYILYGVIIVLINVVGLTLYFRIDLTKNSVYSLSPISKEVVSSLEEPLTIKIFFNKDL
                     PAPYNAVYRYLQDLMVEYDNAGNRYFHYEFVDVEKQKDAASDFGIYPVQIREIRNDQV
                     KFRNAFMGLAIIHGDLIEKIDSITEPEGLEYRITTLIKKMNGKIDSLLKLKNPITVTL
                     YASSNLPIPGMQNLNERVYAEVQKCNIRNYNKIQYRYIDPLQDPQGSTLAQMYGLPTL
                     RWPRFTTMEGKIVEPGEGMVGIVVECNNKFETVQILSRSIFGQYSVGDLTRLEDTLNA
                     AIDNLISINPKVGYIVGHGERDINDEQNGAAQFRKMIGDMYELVTIDITKDEIPDDIA
                     TIIINGPRSMFSEYELYKIDQFLMRGGSAVMLLDSYVEQQMQGMQMFQRPPQALPVFT
                     GLEGMLAHYGVKIGRDIVMDKQCFIAQQRGLGELTIYFAPMISEEGLSKDNEITKYLK
                     KIVFLKASSISVDETLLKENKASVQYVVKSSPYSWLQQGNINFLPWGANPPQDSSLKQ
                     YNLAVLVQGNLKSYFADKQPKIDVKTKETKGKDYVQEEALKQSVKPVKLFVAGTSEIT
                     GRNIIDEEGKSPNAVFMHNVIDYMQGNYAIPQMRSKGLSFNPIKETSDTAKSVIKGIN
                     IAGLPLLVILAGVLYMWRRTIRRRRIEEQFSSMR"
     gene            complement(3899..>4115)
                     /locus_tag="PK348_04155"
     CDS             complement(3899..>4115)
                     /locus_tag="PK348_04155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HXK65440.1"
                     /translation="IGFAICIVLSLITKFLYFISPKLVSLFEYISADYHFQNIARGVV
                     DSRNIIYFASVIAISSMASIYQLEVRR"
BASE COUNT         1165 a          855 c          634 g         1461 t
ORIGIN      
        1 aatgcctcat caatagcctc aggaaacaaa acaaattcac cataaaacac tctatcgcct
       61 gattttacaa aattagctgc ttcttctgct gtgattttct ttttttcata ttcccatttt
      121 tcgatatcac ccattgatct tctcctttct tcaatagcga ttgtactgct ttatattttt
      181 tgattatcat aactttacca aaaatttata ataaatatat tgtcattatt acatcaacat
      241 attgtttgat ttattgatag tatcaataca acatgtttta tttatattga atatagcata
      301 tacaaatttt tatttctaat ttttatatac ctgtaaatac tattaatcaa tagatattga
      361 ataccatctg gcaggtttgt agcgcattta gagcacccat gcatggcgcc tgtaaatggt
      421 caattattta cctttttaaa tcgggagcgt ttaaagaatt ctcttagcct ccattcatcc
      481 aatgaaagaa cataggggct gtcccaaaag aattcgttga gtaaattttg caaacattat
      541 atcataatac tcgctcattt cgatacaatt ttgacacaaa atctggtaaa attactcaat
      601 gagcgttgcg aaatttgtat cgaaacgact tttgggacag cctccaccag tatttatttc
      661 tttaaatctt gaagtgtctt aaaaattctt atagctctcc attcgtctaa tgtaaaaaca
      721 aactgggtat ccatttagga tacccacctc cagtatttat ttctttaaat cctgcaaagt
      781 cttaaaaatt ctcttagctc tccattcgtc taatgtaaag acatactgac tttttgaact
      841 ggtacatata tacctgttgt tttcatattc tttatggata accagcgtaa tgtcttctgg
      901 tttttgtgat tcttcacctt ttaccgaaac ggtaattgaa gcaattttcc ccgtcaatgt
      961 tttggcatta acgtcaggga atgaatatgc tttcagcgga tcaaatgtat ataacatctc
     1021 cttaacttta ttgttatcca gtattactgc tggttgttct ttgcatatcc attgctcttc
     1081 tttttctatt tctttgagct tttcctgttt ttctttttgc gcattacctt cctcgttctt
     1141 cttttctact actttttttt gaagtgttaa cgatgtattt ttataccata actttactgt
     1201 atcaatcttt gctatatcaa gctgaacaat atctttattc cgcaattcat ctactgtttt
     1261 attgaagtat gaatctaacg tatcttctgc aaggtaaacc tttggatcgc cttcaaatcg
     1321 aacatatgtg tggcgatagg tgctcccctt tttgccaata taaacatcac gtacaatctg
     1381 accattgctt tttgcctgaa cccgtattgc atggtcgttg tcaagctcat agcgttcata
     1441 atgtggtttg tcacttatca gatcggtaat cgcgaggtcc tttatcttat ttaccatatt
     1501 ggcaataata ttcttatcgc cttcaaattc atttaccttc cagctatcgc catcctgttt
     1561 tatgagtatt atcgcttccc cttttttaag tacaagctct gttactgggc tttttatggt
     1621 aggcagcgta actacgtttt ttggtatcaa tgaattaatg ataagatacc cagccagaac
     1681 agctatcact gcaatactga taagaacatt ttttttcatg tgcatacaaa cacctcttat
     1741 ctgaattatc tcatactgga aaattgttct tcaatgcgcc tgcggcgaat agtacgacgc
     1801 cacatataca gcacaccagc aagaattacc aatagaggta atccagcaat gtttataccc
     1861 ttaatgacac tttttgccgt atcagatgtt tctttaattg ggttgaacga taggcccttg
     1921 cttcgcatct gtggaatggc atagttgccc tgcatataat caatcacgtt gtgcatgaac
     1981 actgcattgg gcgattttcc ttcttcatca ataatgttac gccctgttat ttctgatgtt
     2041 cctgccacaa atagttttac cggttttaca ctttgtttca aagcttcttc ttgaacatag
     2101 tctttacctt ttgtttcttt tgtttttaca tcaattttgg gctgcttatc ggcaaagtag
     2161 ctttttaaat ttccctgcac caggactgcc agattatact gcttgaggct tgaatcctgc
     2221 ggcgggtttg caccccatgg caaaaagtta atattaccct gctgtaacca gctgtatggc
     2281 gatgatttta caacatattg tacagaagct ttattttcct taagcagtgt ttcatcaact
     2341 gaaatggaag aagcttttaa aaatactatc ttctttaaat atttagtgat ttcattatct
     2401 ttagacaaac cttcctcaga aatcattgga gcaaagtata ttgttaactc acccaaccca
     2461 cgctgctgtg caatgaagca ctgcttatcc atcactatat cacggccaat cttaacgcca
     2521 taatgggcaa gcatcccttc aagaccggta aataccggta acgcctgagg tggtcgctgg
     2581 aacatttgca taccctgcat ctgctgttcc acatacgaat caagtagcat tacagcacta
     2641 cctccgcgca tcaggaactg gtcaatttta tacaattcat attcactgaa catggatcgc
     2701 ggcccattaa taatgatagt tgcaatatca tcaggtattt catcttttgt aatatcaata
     2761 gttactaact catacatgtc gccaatcatc tttctaaatt gtgcggcacc attttgttca
     2821 tcattaatgt cgcgctcacc gtgaccaaca atatacccca cttttggatt gatgctgata
     2881 agattatcaa tagcggcatt tagcgtatcc tctaagcgtg tcaaatcccc aactgaatac
     2941 tgcccaaata ttgaacggga aagtatctgc actgtttcaa acttattgtt gcattccacc
     3001 acaatgccaa ccatgccttc accaggttca actatcttcc cttccatagt agtaaaccgc
     3061 ggccaccgca gcgttggcaa accatacatc tgagccaatg tactcccttg tggatcttgt
     3121 aatggatcaa tatagcgata ctgtatttta ttgtaatttc gtatattaca tttctgaacc
     3181 tcagcataca ctcgttcatt taaattctgc atccctggaa ttggcaggtt actgctggca
     3241 tacaatgtaa ctgtaatagg atttttcaat ttaagcaggg aatcgatctt gccattcatc
     3301 tttttaatta gcgttgttat acggtactcc agtccttctg gttcagtaat agaatcaatt
     3361 ttttctatta aatcaccatg gataatggca aggcccataa atgcgtttct aaattttact
     3421 tgatcattac gtatttcccg tatctgcacg ggataaatac caaaatctga ggcagcatcc
     3481 ttctgctttt caacatcaac aaattcataa tgaaaatacc tgttgccagc attatcatat
     3541 tctaccatca ggtcttgaag atagcggtac acagcattat aaggtgcggg aagatcctta
     3601 ttaaaaaata tctttatggt taatggttct tccagtgatg atactacctc tttacttatg
     3661 ggtgatagtg aatagacact attctttgtt aagtctatac gaaaatataa tgtaagccca
     3721 actacattta ttaacacaat tatcacgcca tagagtatat actgcgaata gctcttttgt
     3781 gtttcagtat cgcctgattt ggtttgctct tttatgtttt gtattacttt tgtatatata
     3841 attgtaatgt tacttttcag gttttgcagc atatcaataa tgtttctttt gttcataact
     3901 atctcctcac ctccagctga tatatgcttg ccattgaact aattgcaatt accgaagcaa
     3961 aatatatgat atttcgcgaa tccaccacac cacgggcaat gttctggaaa tggtagtcag
     4021 cacttatgta ctcaaacagt gaaaccaatt tgggtgaaat aaagtataaa aattttgtta
     4081 taagcgatag tactatgcaa atggcaaaac ctatt
//
LOCUS       DAOSZP010000137         4110 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_17659_length_4110_cov_3.341060, whole genome shotgun sequence.
ACCESSION   DAOSZP010000137 DAOSZP010000000
VERSION     DAOSZP010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4110
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17659_length_4110_cov_3.341060"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..671)
                     /locus_tag="PK348_04160"
     CDS             complement(<1..671)
                     /locus_tag="PK348_04160"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HXK65441.1"
                     /translation="MSLMKSISGIRGVVGESFTPDLITRVAMAFARHIHGGNVIVGRD
                     SRITGQQIAQCFESVLSLCGCQVIDIGVVPTPTVQIMVEHTKAKGGVVISASHNPIQW
                     NAFKLINATGSFFGPDEMNTFLSLVDTITPFGAKWNAIKPVMCDSSAANVHIEKVLAA
                     IDVAAIKKRKFTVVLDSVNGAGSLITQELLKKLGCRVIPIHCDMTTGTFPRGAEPVAQ
                     NLKALS"
     gene            complement(905..3481)
                     /gene="secA"
                     /locus_tag="PK348_04165"
                     /pseudo
     CDS             complement(905..3481)
                     /gene="secA"
                     /locus_tag="PK348_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004758870.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
     gene            complement(3645..>4110)
                     /locus_tag="PK348_04170"
     CDS             complement(3645..>4110)
                     /locus_tag="PK348_04170"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: protein motif:HMM:NF013626.2"
                     /note="catalyzes the formation of glutamate 5-phosphate
                     from glutamate in proline biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HXK65442.1"
                     /translation="MYTKIKAGEMGIQSGEMMIIANGRTEHILQRIMQGEKIGTLFLG
                     KTQLKSKKRWIAFNVKPKGKLIIDDGAVKALKENKKSLLAIGITQIEGNFDLGDAVEI
                     TDKRGTLIAKGIVNYTSGELEKIKGKNTQEIKKIFGSKYYEEVINRDDLIVF"
BASE COUNT         1099 a         1011 c          668 g         1332 t
ORIGIN      
        1 gataatgctt ttagattttg tgcaacaggt tctgctcccc ggggaaatgt accggtagtc
       61 atatcgcaat gaatgggtat taccctgcat cctaatttct tcaacagttc ttgagtaatg
      121 agtgaaccag caccattaac cgaatccagt accaccgtga attttctttt tttgatagcg
      181 gcaacgtcta ttgcagcaag tactttttca atatggacgt ttgccgcaga actatcgcac
      241 ataacaggct ttatggcatt ccactttgca ccaaatggcg taatggtatc aacaagtgat
      301 aaaaacgtat tcatctcgtc aggaccaaag aagctaccag ttgcattgat aagcttgaaa
      361 gcattccact gtatgggatt atgtgatgca gaaataacca caccaccttt ggccttagtg
      421 tgctccacca taatctgtac agtaggcgtt gggaccacac caatatcaat gacctggcat
      481 ccacatagtg acagcacact ttcaaaacat tgtgcaatct gctgcccggt aatcctggag
      541 tcacgaccaa ctataacgtt gccgccatga atatgcctgg caaatgccat tgcaaccctg
      601 gtaatcaaat caggtgtaaa agattcccca accacacccc gaatgcctga aatgcttttc
      661 attaaactca tagaaacctc tttatacact acatagcaaa atcgttatct aacacgtatt
      721 gacgcctgta tagttaaccc cgtctgcttc ttcttgtttt tttgcgcttc acaccgcctg
      781 ttatttctct ggttttatct tcctggctgc gtattgatgg ccgtggtata gcagcaacag
      841 gaattccacc agcaccaaat tgttcaacct ccggttttat ttcctgccca aacggtactg
      901 gaagctcttc aggtttaatt tctatttcct caaccttctg taagcgcact ttcagaagca
      961 tctcaatgat atctactttt agattatcaa gcatttcgtt gaaaagctca aagcccctta
     1021 gcttatactc aaccagcggg ttacgctcac tgtagcctgc tgtccatata ccgtcacgca
     1081 ggtcatccat gaccagcaga tgatcacgcc acttggtatc aatcacctga agcgatacca
     1141 gccgttcaag cgtacgcata tggtcgctgc caacttcctc ttcctttgct ttgtatagtt
     1201 tcagcaattc ttcttttaca caatcaataa gttcatcatg cgataaaacc tcaatatcta
     1261 cccctaacga tccaatatca aatccaaact ttgatctaag aaaaccattc aatccttcaa
     1321 tatcccagtc ttccgaatga cgtttgcctt ctgtaaaata aggaatccag ctttccacca
     1381 catcatcaat atactcacga attttgggcg aaacatcttc accatcaagg attgcattgc
     1441 gttccttata tatatattca cgctggctgt tcatgacatc atcatattcc agcaggtgct
     1501 tgcgtatttc aaagtttctt gcttctactc ttttctgtgc attttcaatg gcacgcgaca
     1561 ccatccggct ttcaatttcc tgtccttcct ccatacccaa acgctgcata acaccggaaa
     1621 tcctgtccga gccaaaaata cgcatcaggt catcttcaag tgaaagataa aaccgtgatg
     1681 accctggatc gccctgcctt cctgaacgcc cacgtaactg attatcaatt cgtcgtgcct
     1741 catgacgttc agtacccaga atgtgcaatc cacctgcctg aaccacttta ttatgattat
     1801 caaaccattc cttaccttta cgtacaatat ccagagcttt gtgctgatcc tgtcctacca
     1861 tggtattggc aaggcttgct gcctcttcaa agttactttt taaaattgct tccctgaagc
     1921 gttcccatag tgttgcatca tgaatgggct tccagttctc cagttcatca atatacgtct
     1981 ttctgccacc aagcacaatg tccgtaccac gacctgccat atttgtggcg atagttactg
     2041 taccagggcg tcctgcttca gcaataatct gggcttcctt ttcatgatac ttcgcgttta
     2101 acacattgtg cggaatgccc actcttttca gcatgtccga taatttttca gatttttcaa
     2161 tagagattgt acctaccagt actggcctgc cctcttcccg caattgctga atttcctgta
     2221 ctatagcatt aaacttttct ttttcggtac gatagatcct gtcaggataa tcggttcttc
     2281 gtactggttc atttgtggga atgacaacaa catctaagtt atatatcttt ttaaattcga
     2341 tagcttctgt atcagctgta ccggtcatac cagctaactt cttatacatc ctgaaataat
     2401 tctggaacgt aatctgtgca aatgtctggc tttcacgcgc tattggcaca ttttcctttg
     2461 cttccagtgc ctgatgcaat ccatcagaat agcgcctgcc gggcataagc cttccagtaa
     2521 attcatccac aataatgacc tgcccgtctt tcacaatgta atcaatgtca cgtttgaata
     2581 aagtatgagc ttttaatgcc tgattaacat gatgcaccag ttctatattg cgactatcgt
     2641 acagattatc tatctttaaa agtttttcaa catgtttcac accttcttcg gtcaaaaggg
     2701 cattgcggtc tttttcgttt acttcataat caacgccctc tttaaggctt ggaataattt
     2761 tatttatcag gtagtatttc ttggtggatt catcagtgga acccgagatg ataagcggtg
     2821 tacgcgcttc atcaatgagt attgaatcaa cctcgtccac aattgcatag taaagatcac
     2881 gctgcactct gagggattta tgttctacca tattatccct gaggtaatca aaaccaaatt
     2941 cattgttggt tccataggtc acatcacagc ggtaagcttt ctggcgtgcg taataatcca
     3001 tattgtgctg aataacacca accgtaagtc ctaaatattt ataaattgga cccatccact
     3061 cagagtcacg gcgagctaaa taatcgttaa cagtaacaac atgaacccct ttacccgcaa
     3121 gtgcattgag atataccggc attgttgcaa ccagcgtttt accttcacct gtcttcattt
     3181 cggcaatctt tccctgatgt aaaacaatgg cacccataag ctggacgtca aaatgacgca
     3241 tgccgatggt gcgccatgca gcctctctta ccatggcaaa cgcatacggt aacagttcat
     3301 ccattggctg accctgctct atgcgccctc tgaactcagc agtttttgcc agcatctggt
     3361 cctgagttaa ttttttaacc tcatcttcta gtgcatttat ttgagctaca atgggctgta
     3421 gctttttaat atcacgttcg tgctttgtac caaaaaacaa acttaaaatt ttatctatca
     3481 tatcatttac ctgtttataa aattatgatt tactatctgt tacataactg ttaatacaag
     3541 tattttttca tgagttacat gtattgtatc taacgttttt attgtagaaa caactatata
     3601 tctgttgtta cataaaagca tgtaatatac ttcttcctca accattaaaa aacaatcaaa
     3661 tcatccctgt taataacctc ttcatagtat tttgaaccaa atattttttt tatctcctga
     3721 gtgtttttgc cttttatttt ttcaagctct ccgctggtat aattaacaat accttttgca
     3781 atcagtgttc ctcttttatc agtaatttct actgcatcac ccagatcaaa attcccctct
     3841 atttgtgtta tgcctattgc cagcaaggat tttttatttt ccttaagtgc cttaacagca
     3901 ccatcatcaa taataagctt tccttttggc tttacattga atgctatcca gcgctttttg
     3961 cttttcaatt gcgttttacc taaaaacagc gtacctatct tttcaccctg cataattcgc
     4021 tgtaagatat gttctgtcct gccattcgca ataatcatca tctcaccgga ttgtatgccc
     4081 atttctccgg cttttatttt tgtatacata
//
LOCUS       DAOSZP010000138         4103 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_17692_length_4103_cov_3.090168, whole genome shotgun sequence.
ACCESSION   DAOSZP010000138 DAOSZP010000000
VERSION     DAOSZP010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4103
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17692_length_4103_cov_3.090168"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1053
                     /locus_tag="PK348_04175"
     CDS             <1..1053
                     /locus_tag="PK348_04175"
                     /inference="COORDINATES: protein motif:HMM:TIGR02231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HXK65443.1"
                     /translation="KEEKERLEKELSLLNTDEATKSLQLVVVHTGQDTATVMIQYITG
                     NAGWNMEYEVRTNTETQTVEVVCNARVYQRTGEDWKDVSLELSTSKPFVATQKPVLNP
                     WYLDVYQPSYYYKESAKSMKLRSEDAAEAPQEAEEMPAMQEERIAFSFQIKGAKTIPS
                     DGNAYKIPVAKATTDCAVRYATVPRVLPYVMLEGEFQNPFDFPMQGGNMNVYLDGKFV
                     NSFNVNKLYVPKDLIPVALGVDESLMVERKLLSKKTEYKGLVSKTKRIEYVYSIKLAN
                     GKKRDVTITVQDAIPVSQNEKITVTLINKNSIGATIDNEGKATWQVQLSPNQKKELTI
                     HFAVEYPESIQVSGLE"
     gene            1060..2199
                     /gene="rlmD"
                     /locus_tag="PK348_04180"
     CDS             1060..2199
                     /gene="rlmD"
                     /locus_tag="PK348_04180"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HXK65444.1"
                     /translation="MKKHRKVLLSIADRFADKRIEPPCMYAGNCGGCALQYVDYSTQL
                     EIKKEYCNTLLNTVATIDAIEPSIPLGYRNRMDYVTAFGKIGLRRRGSFKHVVDLHDC
                     LLLQDKSKRIFAIIREKLSGVQDYDYLVHKGYLRYVVLRQGFNTGQVMVNFVVARQEN
                     NLESVIDAIYNDVDSMSLILSDGMADVSYGKVFEDIKKGYIEESLDGIIYRITPNSFF
                     QANTSITQVMYREIAKQVYGNVLDMYCGVGSISLFVAKQAESVTGVEANHEAVTVAGH
                     NATFNGINNVLFVQGDALEYISGTAVKYDVAIVDPPRTGLGPKVVDKLLQTGLKRIVY
                     MSCNPVTFAQDAAMLVEKYALAWCKAYDMFPQTPHLELLAVFEKK"
     gene            2202..3359
                     /locus_tag="PK348_04185"
     CDS             2202..3359
                     /locus_tag="PK348_04185"
                     /inference="COORDINATES: protein motif:HMM:NF013347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HXK65445.1"
                     /translation="MNYSTSQNAIKRRITQHVIHKPHTFFAVVQPGFEETAHKELTLL
                     GIKECAMTKGGVTFRGTLEHCYTANYYSRVITRILMRLCHFRAYHFNEIYRTLKEFPW
                     ELYIQGHTIDVEVSVHKSKLHHTERIGHEVIKAIEKRLSKFAISSNADSVQKVFVRFD
                     HDECMVSLDSSGEPLYKRGYKQHISEAPLRETLAAALLYECNVFDYDTIIDPMCGSGT
                     FPIEAWCMLHAIPPGANRTFAFMSWPSFRKHAFTGITHNESGEHRDITIIAGDSNPDM
                     VAITQQNSMKAGAQLHTYTGDFLKEKKVIGSGKVLCTINPPYGKRIHTDDTIQLYKRL
                     EYIFNKWYPSWSFCVLIPSKIKHIVTIPTDKEIHFSHGGLPVSALIVYRGR"
     gene            complement(3456..3980)
                     /locus_tag="PK348_04190"
     CDS             complement(3456..3980)
                     /locus_tag="PK348_04190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65446.1"
                     /translation="MENKRLFLLPFVQDILHNEDFQSLRRYTHHGTTTCYHHTLEVAW
                     LAYRLAVALKLDHVAATRGALLHDFFMYNWRTGGPRLHGFRHPRIALNNASKHFVLGP
                     KEKDSILRHMWPLTPLPPRYPESWIVTVADKIVSTMDYFRFVKSKVSLMYNTIHTCMW
                     LPLLSRFNRQKQYS"
BASE COUNT         1274 a          745 c          931 g         1153 t
ORIGIN      
        1 aaagaagaaa aggagcgcct ggaaaaagag ctgtcgcttc ttaatacaga tgaagctaca
       61 aaatcattac agcttgttgt ggtgcatact ggtcaggata ctgcaacagt gatgattcag
      121 tatattacag ggaatgcagg atggaatatg gaatatgaag ttcgtacaaa tactgaaaca
      181 caaacagttg aagtggtatg taatgcccgt gtgtatcaaa ggactggtga agactggaag
      241 gatgtttctt tagaactatc gacctcaaag ccgtttgttg caacccagaa gcctgtgctt
      301 aacccgtggt atttagatgt ttaccagcca tcgtattatt ataaggaatc ggccaaaagc
      361 atgaaactca gaagtgagga cgctgctgaa gcaccgcagg aagctgaaga aatgcctgca
      421 atgcaggaag agcgtattgc attttcattt caaataaagg gtgcaaaaac aataccatcc
      481 gatggtaatg catataaaat acctgtggca aaggcaacaa cggattgtgc agtgcgctat
      541 gcaactgttc ccagagtact tccgtatgtt atgcttgaag gtgaattcca aaatcctttt
      601 gatttcccca tgcagggtgg gaacatgaat gtatatctgg acggcaaatt tgtcaattcg
      661 ttcaatgtta ataaactcta cgtaccaaaa gatttaatac cagtggcact gggtgttgat
      721 gaaagcctta tggttgaaag aaaactttta agtaaaaaga ccgaatataa agggctggta
      781 agtaaaacaa aacgcattga atatgtatat tcaataaaac ttgccaatgg aaaaaagcgt
      841 gatgttacta ttaccgtcca ggatgcaatt cctgtttcgc aaaatgaaaa gataacagtt
      901 acccttatca acaaaaacag tattggtgct accattgata acgaaggaaa agctacatgg
      961 caggtacagc tttcccccaa tcagaaaaaa gaattaacaa tacactttgc tgtggaatat
     1021 cctgaatcta tacaggtttc tggattggag taatagccct tgaaaaaaca tcgaaaggtt
     1081 ttattgtcca ttgctgacag gtttgctgat aaacgcattg agccaccatg catgtatgca
     1141 ggcaactgtg gtggctgtgc cctgcagtat gttgattaca gtacacagct tgaaataaag
     1201 aaagagtatt gtaataccct gttgaatact gtagctacta ttgatgccat agaaccttct
     1261 ataccccttg gatatagaaa tcgcatggat tatgttacag cttttgggaa aattggtttg
     1321 cgaagaaggg gaagctttaa acatgtagtt gatctgcacg attgtcttct tttgcaggat
     1381 aaaagcaagc gtatatttgc cataatacgt gaaaaacttt caggtgtaca ggattatgat
     1441 tatctggttc acaaagggta cctgcgctat gtggtattgc ggcagggatt taatacaggg
     1501 caggtcatgg taaattttgt agtggcacgg caggagaaca atcttgaatc agtgattgat
     1561 gctatatata atgatgttga ttccatgtcg cttattttga gtgatgggat ggcagatgta
     1621 agttacggga aagtatttga ggatataaag aagggatata ttgaagagtc gctggatggt
     1681 atcatctacc gaataacacc caattcattc tttcaggcaa atacatctat aacacaggta
     1741 atgtacagag aaattgcaaa acaagtctat ggcaatgtgc ttgatatgta ctgtggtgtg
     1801 ggaagcataa gcctttttgt tgctaaacag gcggagtctg ttacgggtgt tgaagcaaac
     1861 catgaagcag ttacggtagc agggcacaat gccaccttca atggaatcaa caatgtattg
     1921 tttgtacagg gtgatgcgct tgagtatata agcggtactg cagtgaagta tgatgtagca
     1981 atagttgatc caccgcgaac aggtcttgga ccaaaagttg tggacaaatt gttgcagaca
     2041 ggcctgaaaa gaattgtgta catgtcatgt aatcctgtta cctttgcaca ggatgcagca
     2101 atgcttgttg aaaaatatgc acttgcatgg tgcaaggcct atgatatgtt tccacaaact
     2161 cctcatcttg aattacttgc agtgtttgaa aaaaagtaac tatgaattac agcacctcac
     2221 agaatgctat caagcgccga attactcagc atgtgatcca caaaccccat acattttttg
     2281 cagtggtaca gcccgggttt gaagaaacag cccataaaga gctaacgctt ttaggtataa
     2341 aagagtgtgc aatgacaaaa ggcggagtga cattcagggg aacgctggaa cactgttaca
     2401 cagccaacta ctattcacgg gttataacgc gtatactcat gcggttgtgc catttcaggg
     2461 catatcattt taatgagatc tatcgcacac ttaaagaatt tccgtgggaa ctatatatac
     2521 agggacacac aattgatgtt gaggtaagtg tccataaatc aaagctgcat catactgaac
     2581 gaattggcca tgaggtaata aaagcaatag aaaaacgcct gtccaagttt gccatatcga
     2641 gcaatgctga ttcagtacaa aaggtatttg tgcgctttga ccatgatgag tgcatggtaa
     2701 gtttggattc aagcggtgag ccgttataca agcgtgggta taagcagcat atcagtgagg
     2761 caccacttcg tgaaacactg gctgcagcac tattatatga atgtaatgtg tttgactacg
     2821 ataccatcat tgatcccatg tgcggcagtg gcacattccc cattgaggca tggtgcatgc
     2881 ttcatgcaat tccaccagga gctaaccgta catttgcctt tatgagctgg ccgtcattcc
     2941 gcaagcatgc attcaccggt attacgcata acgagtcagg tgagcacagg gatataacaa
     3001 ttatagctgg agacagtaac cctgatatgg tagctataac acaacaaaac agcatgaaag
     3061 caggtgccca gctgcatacg tatactggcg attttttaaa agaaaaaaaa gtgattggta
     3121 gcggtaaggt tctgtgcact atcaatccac cctatgggaa gcgtattcat actgatgata
     3181 ctatacaatt atataaacgc cttgagtata tttttaacaa atggtatccg tcgtggagtt
     3241 tttgtgtatt aatcccttca aagattaaac acatagttac tatccctact gataaggaaa
     3301 tacatttcag tcatggaggc ctgcccgtta gcgctttgat tgtgtacagg gggcgatagt
     3361 tcctgcaaac ttagcgtgtg aatatcatgc agactgctat gatgccacat gagagatcac
     3421 gtgcgtttta acaaaccagt aagcaataaa gtgatttaag aatactgctt ctgacggttg
     3481 aatcgcgata gtaatggtaa ccacatgcag gtgtgtatgg tattatacat caacgatact
     3541 ttgcttttta caaagcgaaa gtaatccata gttgatacta tcttatcagc tactgttact
     3601 atccatgatt caggatagcg gggtggaagt ggcgtaagcg gccacatgtg gcgcaggata
     3661 ctatcttttt cctttggacc cagcacaaaa tgttttgatg cattattgag tgcaatgcgt
     3721 gggtgcctga atccatgaag tcttgggcca cctgtgcgcc agttgtacat aaagaaatca
     3781 tgcaacaatg ccccgcgtgt tgcggcaaca tggtcaagtt ttaaagctac agctaatcta
     3841 tacgctaacc atgctacctc cagtgtgtgg tgatagcatg ttgttgtgcc atgatgtgtg
     3901 taacgccgta gtgattgaaa atcctcattg tgaagtatat cctgtacaaa tggcaataag
     3961 aacaaccttt tattttccat aattctcctg tatgctggta ttacctgtga ttttcgtttt
     4021 tattacgaat ggtgctgatt agtgcagtat atctcttttt gaataattga ataattttat
     4081 ttgcgttcag gggctttata gta
//
LOCUS       DAOSZP010000139         4097 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_17716_length_4097_cov_6.283276, whole genome shotgun sequence.
ACCESSION   DAOSZP010000139 DAOSZP010000000
VERSION     DAOSZP010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4097
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17716_length_4097_cov_6.283276"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            275..508
                     /locus_tag="PK348_04195"
                     /pseudo
     CDS             275..508
                     /locus_tag="PK348_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612369.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6444 domain-containing protein"
     gene            654..1556
                     /locus_tag="PK348_04200"
     CDS             654..1556
                     /locus_tag="PK348_04200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65447.1"
                     /translation="MRKSMLSIMVLIFMMSASKVFATDINIGTVPLPNLYIAAPPIQL
                     PWFEMYVGKEQSGDLGVCYSSVQGDAIDGKHVGIGFVGLGNIWGPYIGIAIGTYCDFS
                     WGNLYDNDKLDYINFNIRIPVSLVIQVVRIDNAAINIYGGGDINGKSTWFDLPTVTTG
                     NSYDTYKLTSLAGSFPLGYHVGLQASGRYAGLAITALAGMQSIYFKESKYEYENTDDD
                     NTYYFKIPAETIKNYFYGIKLSVIPWDISLSYTYQKFDKSDKTGFPEFSQHTFMITFL
                     VAGKHADVKEEKKEEKPKQQVDAV"
     gene            1620..2600
                     /locus_tag="PK348_04205"
     CDS             1620..2600
                     /locus_tag="PK348_04205"
                     /inference="COORDINATES: protein motif:HMM:NF012803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HXK65448.1"
                     /translation="MNSAKSFVNMIAISSLLIIGGCVSQQEVKPVKKFPFPEPIVYNI
                     AFCKPVNVYTKEKADAILQNLQPYIYQLRSEDVLAKTDDVIVDNEAIKINWNWEELQT
                     ETLSQKSTTSQENDVTGDVIKGILSGKKEDMIGALISGAANSSSQTTTTNMQVTKKVE
                     KHNKTIIMFEDVSNIFRFMSNYIIIRLKSGEDVYIICNNEYYLQKWIDAFMSAMKARG
                     YNTDFTLGVMCQKQPMNAEQIDYLQVNSGAVVLGIMPGSPAAEMGLEYLDVITKVNGM
                     AISSGEELTAYIKSKKPKNNERLTFEIVKSKREGEEVSKVVIRKDLIVKN"
     gene            complement(2597..>4097)
                     /locus_tag="PK348_04210"
     CDS             complement(2597..>4097)
                     /locus_tag="PK348_04210"
                     /inference="COORDINATES: protein motif:HMM:NF025925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HXK65449.1"
                     /translation="ENRSVTVPAGTTIADALLACNIILHTPCGGKGICKKCTVHASGL
                     LSQPDDYEKSLGDSSLRLACRAVIIGDAYIWIDSITQRPRYPELNPTHTYAVAIDIGT
                     TTVQIAFVDMTTKQAMPEITLLNPQRRFGHDVMTRISHANDPAKASQLTQILLQTIIT
                     TIQDVCNISGIDIHSIVTIAISGNTVMSYSFLGIDITPLGTYPYTTLITSFDNYNHYP
                     PLKKTFPNAHITVLPVVSAFLGGDFLGGVGTLPKTKGNSFFFDIGTNGEMAIIKEDGT
                     IVATSCAMGPALEGMNISSGMTATEGAITHFTLYNNTLTYTSLGDGKPSGISGTALID
                     LIAILLRNGVIEKSGAIKTAIPQPLSKHIQLEHIENMKTLIILDIITINQQDIRNVQL
                     AKGASLAAAKILLREAGLSAHDIEQVYIAGAFGQHINIDNFNELSFLPEFKQATYHCA
                     GNTSLKAAITACTDNEFLQRLLQRKEHITTIDLSQHHEFNDIYIKSLDF"
BASE COUNT         1335 a          621 c          876 g         1265 t
ORIGIN      
        1 tttttagcgt tggaagtatg attttttaga atttttattt ttgttcagag gcttccgtta
       61 taaaaatatc ttgacaaatt gatggtaata atgtattact tatcttcata ttgagggaaa
      121 cgcaactact cagctaaaat ttttcacgta gaaaaaaatt ttgcttttta ttgtagaggc
      181 atattgtaga ttatgtttca taaaataaga agaagctgaa acgatatatg aacaggggaa
      241 acaagcagtt gttgattgta ttcttaaatg agttgacagg ataggaagac ttgaaagtca
      301 acttaataca aacactcata acagcagcaa agcgccatca agtgatggat tgaagaaaaa
      361 ggcaaggacg cgaagtcaaa ggaagcgaac gggggaaaaa acccgcagga agtctgaggc
      421 atttgatgtg ataggtatag taccatctta ccaacattgg aaggagcata tgcactccac
      481 aaaagtattg gagcatcaat cgatttagaa cagctggaaa agtatgaaaa ggaatataca
      541 cacattctac gtgaagggtt tagggttttt ttcttaatag ctgaatagtt actaatattt
      601 aaggtttaag ggaaatattt aatcatcatc aattttatct ggaggatagt gtcatgagga
      661 aatcaatgct gagtattatg gtgctaattt ttatgatgtc ggcttcaaaa gtttttgcaa
      721 ctgatattaa tattggtact gttccacttc ccaatttata tattgcagca ccgccaattc
      781 aattaccatg gtttgaaatg tatgttggaa aagaacaatc aggtgacttg ggtgtttgtt
      841 atagtagtgt acagggtgat gcgattgatg gcaagcatgt aggaatagga tttgtcgggt
      901 tagggaatat ttgggggcct tatataggga tagcaattgg aacctattgc gatttcagct
      961 ggggaaattt gtatgataat gataagcttg attacataaa tttcaatatt agaatacctg
     1021 tgtcactagt aatccaggtt gtcaggatag ataacgctgc aatcaatata tatggcggag
     1081 gtgatattaa tggcaaatct acttggtttg atttgcccac tgtaactact ggtaactctt
     1141 atgacactta taagctaaca tctttagcag gttcgttccc attagggtac catgtagggc
     1201 ttcaggcaag cggtaggtat gcaggactag ctattacggc acttgcaggg atgcaaagta
     1261 tatatttcaa agaaagtaaa tatgaatatg aaaacacaga cgatgataat acctactatt
     1321 tcaaaatacc tgctgaaaca ataaaaaatt atttttatgg tataaagtta agtgtaatcc
     1381 catgggatat ttcattaagt tacacctatc aaaaatttga taagtcggat aagacaggat
     1441 ttcctgaatt ctcacaacat acatttatga ttacattttt agttgctgga aaacatgctg
     1501 atgttaagga agagaaaaag gaggaaaaac caaaacagca ggttgatgct gtataaattt
     1561 aaaggttgta aactatggtg gtactttttt ttatgtcaat ttaacttaat gaggttaata
     1621 tgaattctgc taaatcattt gtgaatatga ttgccatatc ttcgttatta ataatcggtg
     1681 gctgtgtatc acagcaggaa gttaaacctg taaaaaaatt tccgtttcct gaaccaatag
     1741 tatacaacat agctttttgc aaaccagtta atgtttatac taaagagaag gcagatgcca
     1801 ttttacaaaa tttgcagcct tacatttatc agttgcgtag tgaagatgtt ttggcaaaaa
     1861 ctgatgatgt aattgtggat aacgaagcaa taaagataaa ttggaattgg gaagaactgc
     1921 aaactgaaac cttatcccag aaatcaacaa cttcacagga aaatgatgtt accggtgatg
     1981 tcataaaagg gatattaagc ggaaaaaaag aggatatgat tggagccttg atatcaggag
     2041 ctgcaaattc atcatcgcaa actaccacaa ccaatatgca ggttacgaaa aaagttgaaa
     2101 agcataataa aacaatcatt atgtttgaag atgtgagtaa tatattcaga tttatgtcta
     2161 attacattat tattagatta aaaagtggtg aggatgttta tataatatgt aataatgaat
     2221 attatctgca gaaatggatt gatgctttta tgtctgcaat gaaagcacgc ggttacaata
     2281 ctgattttac actgggtgta atgtgtcaga agcaaccaat gaatgctgag caaatagatt
     2341 acttgcaggt caatagcggt gcagtggtat tgggcattat gccaggcagc cccgcagcag
     2401 aaatgggtct ggaatatctt gatgttataa caaaagtaaa tgggatggca atatcatcgg
     2461 gtgaagagtt aacagcgtat ataaaaagca aaaagcccaa aaataatgaa cggttgacat
     2521 ttgagattgt aaaatcaaaa agagaaggag aagaggtatc aaaagttgtt attagaaagg
     2581 atctgattgt aaagaattaa aaatccagtg atttgatata tatatcatta aattcatgat
     2641 gctgtgataa atcaatggtt gtaatatgtt cttttctttg tagtaacctt tgtaaaaatt
     2701 cattatctgt gcatgcagta attgctgctt ttaggcttgt gttccctgca caatgatagg
     2761 ttgcttgttt aaattcaggc agaaaggata attcattaaa gttatcaata tttatatgtt
     2821 gcccaaatgc accagctata tacacctgct caatatcgtg agctgacagt cctgcttcac
     2881 gtaataaaat ttttgcggct gcaaggcttg ctccttttgc aagctgaaca ttgcgaatat
     2941 cctgttgatt gatggtaatg atatcaagaa tgatcagggt tttcatgttt tcaatgtgtt
     3001 caagttgaat gtgctttgat agcggttgtg gtatagcagt ctttatggca ccactttttt
     3061 caataacacc attcctgaga agaatagcaa tgagatcaat aagtgcagtg ccgcttatcc
     3121 ctgatggttt gccatcacct aacgaagtat aggttaatgt attattgtaa agagtaaagt
     3181 gggtgatagc tccctcggtt gcagtcattc cacttgatat attcattccc tccaaggctg
     3241 gccccatagc acatgatgta gcaacaatag tgccgtcttc ttttataatt gccatctcac
     3301 cgtttgtgcc aatgtcaaag aaaaagctgt tgccttttgt ttttggaagt gtaccaacac
     3361 cacctaaaaa gtcaccaccc agaaatgcac tgactactgg taaaacggtt atgtgtgcat
     3421 tgggaaatgt tttttttaaa gggggataat ggttatagtt gtcaaaagac gtgatgagtg
     3481 tggtataggg ataagtacct aaaggggtaa tatcaatccc taagaaggaa taactcatca
     3541 cagtgttgcc actgatggcg atagttacta tggagtgtat atcaatacct gaaatgttgc
     3601 atacatcctg aatagtggtt attattgttt gtaaaagaat ctgtgtaagt tgactggctt
     3661 tagcagggtc atttgcatgg cttattcgcg tcataacatc atgaccaaat cgtcgttgtg
     3721 gattaaggag agtaatttcg ggcattgctt gcttggttgt catatcaaca aaagctatct
     3781 gcactgtggt ggtaccaatg tcaattgcca cggcgtaggt gtgggttggg ttaagctctg
     3841 gatagcgagg acgctgtgta atgctgtcaa tccagatata tgcatcgcct ataattacag
     3901 cacggcaggc taatctcaag gaactatcgc ccagactttt ttcataatca tccggctggg
     3961 ataataaccc tgaagcgtgt accgtgcatt ttttgcatat accttttcca ccgcatggcg
     4021 tgtggagtat aatattgcaa gcaagcaatg catcggctat ggtagtgcca gcgggtactg
     4081 ttacactccg gttttca
//
LOCUS       DAOSZP010000140         4011 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_18167_length_4011_cov_3.008089, whole genome shotgun sequence.
ACCESSION   DAOSZP010000140 DAOSZP010000000
VERSION     DAOSZP010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4011
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18167_length_4011_cov_3.008089"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1136
                     /locus_tag="PK348_04215"
     CDS             <1..1136
                     /locus_tag="PK348_04215"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HXK65450.1"
                     /translation="KEPLGRKLTRNVGMMLRGLGMMDLRDDVVARGLASEEVTIAEAL
                     KLAGYKTGMVGKWHLGDYSTQPEFNPLRHGFDFYYGVPHSNDMRPCPVYRNETKVMDN
                     IHGEDQSFLTGAYTKEALQFIESCGNNPFFLYFAHTFPHQPLWASEKFDKKSPAGKYG
                     DAVEEIDWSVGQILKLLKEKGLDNRTMVIFTSDNGPWYEGSTANLRGRKGNSNEGGFK
                     VPFIVKWPGVVPQGKITHEPAMNIDLFPTLLGLAGVGLPEDRIIDGKDILGLFKGNDK
                     PVHEDLYFYHYDLLVGIRSGKWKYYSRIDRYVWPNPLDSTPLIDKLGKDALGHRWPLL
                     YNLEKDPGEAYNVLDTYPDIARKLRQKLENWEKETMKNPRGFK"
     gene            complement(1137..1766)
                     /locus_tag="PK348_04220"
     CDS             complement(1137..1766)
                     /locus_tag="PK348_04220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65451.1"
                     /translation="MNLNHPEATQQALSILRSGNPFQWYVITMLAFVVYIYFNEIQNK
                     NWKGIAAGLSLYMVHWFVEIINALIQHFTGHALWTVPTGTAFLILIGVGVELSLMFSV
                     SGLIFSKILPEDPKAKILGINNRLFIAIANAAFYSIFEIFLVKTPCFVWVYPWWGALP
                     VFITVYVPFWVVSLYCYDWQPKVQKAVIGSLFAINAGMLVVFAGILKWI"
     gene            2119..2775
                     /locus_tag="PK348_04225"
     CDS             2119..2775
                     /locus_tag="PK348_04225"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HXK65452.1"
                     /translation="MVKNIFINGLIIMIAICSLHCKKDESIKPANAIIVLAQGVVSIN
                     NIQAHTGVSLHYGDVIKTGDDGICRIQIGDRNVLQIGKSSELVFNISDTGNELLLNQG
                     WLGGVTRKIFTSGGKYLVKTPTVVASIRGTSFCVVATSAEKSYFCVCNGSINLMGSSM
                     EKGEDVTAAHHKGLWFKKDKNGNVVVKEAGLLFHDDKGIEELAASINQTIDWTKPDTY
                     "
     gene            complement(2988..3971)
                     /locus_tag="PK348_04230"
     CDS             complement(2988..3971)
                     /locus_tag="PK348_04230"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HXK65453.1"
                     /translation="MADSIYRELSKRLMFENSQLLQQIWQRVANETEAEIICYLPANA
                     AQVAEKFNLSVDEATRILEELFKRGAAFKKSKPKGAEYRPPKHIVQFHDATLLWKGAP
                     ADFKKLWVEFMNTEYPPLLEVVTKAGIPSFMRVIPINATIQPQSQVLVYEDAYRMIEQ
                     AKELAVVDCVCRLSHGNCNKPLNVCIQVNNGATYTLERGTGRAITKEEAFEILKISEE
                     AGLVHMTENKGFGNAICNCCSCCCEMLRFAGNRATHGVVAKSRFTATVHATNCNGCAL
                     CTSVCPVHAIEVNEVATVDAEMCIGCGLCATQCPSGAITLHETRPKEHIPV"
BASE COUNT         1221 a          725 c          854 g         1211 t
ORIGIN      
        1 agaaagaacc gttggggcgc aaactaacgc gtaatgtagg tatgatgctg cggggattag
       61 gcatgatgga tttgcgggat gatgttgttg ccaggggatt ggcttctgaa gaagtaacta
      121 tcgccgaagc tttaaaactt gccggatata aaaccggtat ggttggcaaa tggcatctgg
      181 gcgactattc aacacagcct gaatttaatc cactgcgcca tggttttgat ttttactatg
      241 gtgtaccaca cagcaacgat atgcgaccat gcccggtata tagaaatgaa accaaagtaa
      301 tggataatat tcatggagaa gaccagtcat ttttaaccgg tgcatataca aaagaagcct
      361 tgcagttcat tgagtcgtgt ggcaacaatc cattcttttt atattttgcc cacacatttc
      421 ctcatcagcc actgtgggct tcagaaaaat ttgataaaaa gtcacctgcc ggtaagtatg
      481 gggacgctgt tgaagaaatt gactggagtg ttggacagat cctgaaactt ctgaaagaaa
      541 aaggtctgga taacaggact atggtgattt ttaccagtga taatggtccg tggtatgaag
      601 gaagtacagc taatttgcgg ggcagaaaag gaaatagtaa tgaaggtgga tttaaggtgc
      661 cgtttattgt aaaatggcct ggagtggtac cacagggcaa aattactcat gagcctgcaa
      721 tgaatattga tcttttccca acgttacttg gacttgcagg tgttggattg cctgaagatc
      781 gcatcattga tggaaaggat atactggggc ttttcaaagg taacgataag ccagtgcatg
      841 aagatttata tttttatcac tatgacctgc ttgttggcat tcgttccggg aaatggaagt
      901 actattcaag gattgatcgg tatgtatggc ctaatcctct tgattccaca ccccttattg
      961 acaaattggg gaaagatgca ctgggacacc gctggccatt gctgtataat ctggaaaaag
     1021 atcctggtga agcatacaat gttctggata cctatcctga tattgcccgt aaactacgcc
     1081 agaagcttga aaactgggaa aaagaaacta tgaaaaatcc cagagggttt aagtaattaa
     1141 atccatttta agattccagc aaaaacaacc agcattcccg cgttaatggc aaagaggcta
     1201 ccaattactg ctttttgtac ttttggctgc cagtcatagc agtacagcga tacaacccag
     1261 aatggtacat acacggtgat aaataccggc agtgcacccc accatggata tacccacaca
     1321 aagcatggcg ttttaactaa aaatatctca aagattgaat aaaaggcagc attggcgata
     1381 gctataaata aacgattgtt gatgccaaga atctttgcct tggggtcttc tggtaaaatc
     1441 ttgctgaaga ttaaaccgga tactgaaaac attaagctta attccacgcc cacaccaatc
     1501 agtataagaa atgcagtccc cgtgggtacc gtccacaatg catgcccggt aaaatgctga
     1561 atgagtgcat taattatttc cacaaaccag tgaaccatgt aaagcgaaag ccctgctgca
     1621 atgcctttcc agtttttatt ctggatttca ttaaagtaga tatagacaac aaaagctaac
     1681 atggtaatga cataccactg aaaagggttg ccactgcgta aaatactgag tgcctgctgt
     1741 gttgcttcag gatgattgag attcataatt cctccccatg aaataagatt tatgattttt
     1801 gatagtgctg ttcatacttg catgacaatc tttgggaata tcatgcacca tacgttttgt
     1861 cttcctcacc atgtgaattg cttataatga aatacgtcac gtattttcat gttttcaatg
     1921 gttgctatgc agcaatagtc tggttaccat actattacat ctcattatta taagaagtgt
     1981 atagtgttta ttatgaattg ttaagtattg gataaaaaca ttattacaaa tggaagggga
     2041 atgcaagaaa tattttcatt gactttcctt tttttatatt tatgatatgt ttaatgaatg
     2101 atagtaatgg aggatcgtat ggtaaaaaac atatttatta atggtcttat aataatgata
     2161 gcaatttgca gtttgcattg taaaaaggat gaatcaataa aaccagcaaa tgctatcatt
     2221 gttcttgccc agggtgttgt cagcataaat aatattcagg cacatacagg ggtatcatta
     2281 cactatggtg atgttattaa gactggtgat gatggaatat gcagaataca gattggtgac
     2341 aggaatgttt tgcaaattgg taagtcttct gaattagtgt ttaatatatc tgatacaggg
     2401 aatgaactgc tcttgaacca gggatggttg ggaggagtta cccgaaagat ttttaccagt
     2461 ggtggtaaat atttagttaa aacgccaacg gttgttgcaa gtatacgagg tacttcattt
     2521 tgtgttgttg ctacttctgc tgaaaaatca tatttctgtg tatgtaatgg aagtattaat
     2581 ttaatgggaa gcagcatgga aaaaggagaa gatgtaacag cagcccatca caaaggatta
     2641 tggttcaaga aagacaagaa cgggaatgtt gtagttaaag aggcgggttt gctgtttcat
     2701 gatgacaaag gcatagaaga gcttgctgca agcatcaacc aaactataga ttggacaaaa
     2761 cctgatactt attaagcatg tatgttagtc aaaatcatga cttattccag atataatggt
     2821 cttgttatat aattttatca tacatgtttg taaagtaata actgatcaac aattttgaac
     2881 acattacata aaataggtac aataatgtga attgaagtgg aaaaggggtg ctttccattt
     2941 atatcataaa aattgagtta ccgataatta ctatataatc ttaaagatta taccggaatg
     3001 tgttctttgg gtcgtgtttc atgaagcgtt atggctcctg atgggcactg tgttgcacac
     3061 aatccgcatc ctatacacat ctcagcatca actgttgcta cctcattcac ctctattgca
     3121 tgtacaggac acacagaagt gcatagtgca caaccattgc aattagttgc atgtacggtt
     3181 gctgtaaatc tgcttttggc aactacaccg tgagttgccc tgttacctgc aaaacgcagc
     3241 atctcacaac aacaggagca acagttacaa atggcgttgc caaagccctt gttctcggtc
     3301 atatgtacca ggcctgcttc ctcagaaatt ttgagtattt caaaagcttc ctctttggtt
     3361 attgccctgc ctgtaccacg ctccagtgtg taggttgcac cgttgttaac ctgaatacat
     3421 acattgagtg gtttgttaca gttgccatga gaaaggcggc atacacaatc aactactgcc
     3481 agttcttttg cctgttcaat cattctgtat gcatcctcat aaaccagaac ctggctttgt
     3541 ggctgtatgg ttgcattaat gggaataacg cgcataaatg agggtatgcc tgcttttgtt
     3601 acaacttcaa gcaatggtgg gtattcggtg ttcataaatt ctacccacag ttttttaaaa
     3661 tcagcggggg cacctttcca cagtagtgtt gcatcatgga actggacaat atgtttgggg
     3721 ggtcggtatt ctgctccttt gggttttgat tttttaaatg ctgcacctcg cttaaataat
     3781 tcttcaagta tgcgtgtagc ctcatccacg gaaaggttaa acttttcagc tacctgggct
     3841 gcattagcag gcagataaca aataatctct gcttcagttt catttgccac gcgctgccag
     3901 atctgttgta aaagctgaga attttcaaac ataagacgtt tggataattc tcggtagata
     3961 ctatctgcca tacttcctcc taattatttt tgttactaat tgtaaatagt t
//
LOCUS       DAOSZP010000141         3998 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_18229_length_3998_cov_2.865331, whole genome shotgun sequence.
ACCESSION   DAOSZP010000141 DAOSZP010000000
VERSION     DAOSZP010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3998
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18229_length_3998_cov_2.865331"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..1807)
                     /gene="fdnG"
                     /locus_tag="PK348_04235"
                     /pseudo
     CDS             complement(1..1807)
                     /gene="fdnG"
                     /locus_tag="PK348_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011365770.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
     regulatory      complement(2070..2194)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            2340..3197
                     /locus_tag="PK348_04240"
     CDS             2340..3197
                     /locus_tag="PK348_04240"
                     /inference="COORDINATES: protein motif:HMM:NF016131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HXK65454.1"
                     /translation="MPMIHHTEHLEQRKESLLNNSMFSEDLFALYRSLFSLQFQSLQH
                     LASIITDSKLIVDTTQYPMIQPHTLQFTDEIIADIKKMFKNIAALITQYQTAYSFDTL
                     LANLDDNPELYKDIITALLSKDSTFEAIAHNNKLDTDELIFVTVNAYKPLFIALKDAA
                     LQDITQFPDYSEGLCPFCGFLPDMAKIVEPKNNKRFLHCALCECEWEYKRVKCVACGN
                     ENTDTLGYYSYEPDEKYRFDYCNKCNTYIKTIRITKQHDESQFDLTVENLITNFLDAS
                     ALQMGYTRQ"
     gene            3201..3782
                     /locus_tag="PK348_04245"
     CDS             3201..3782
                     /locus_tag="PK348_04245"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HXK65455.1"
                     /translation="MEAIEACILAGGKSSRFGSDKTLHLFKGRPLIEHVLHSLKPLFQ
                     TISIIANDTDKYNYLTIPVYPDIIPGLGPLGGIYTALKCSATDYIFVCAADMPFLNQE
                     FIAFMLQIPHMYDCIIPCWAGNTEPLHAVYSKQCIPHIEKLIKEKKYKISFLFEQVRM
                     RYIMDDELIFYAEDPSELFYNINKQSDIPPSFQ"
     gene            complement(3776..>3998)
                     /locus_tag="PK348_04250"
     CDS             complement(3776..>3998)
                     /locus_tag="PK348_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65456.1"
                     /translation="NIGGHLVWEYEYWNLSFKGVWLIFLFGYFHFFVFAIIVIGLSTI
                     KKKIIAVSCIYAVPIIMNIVALGILGWNY"
BASE COUNT         1221 a          894 c          690 g         1193 t
ORIGIN      
        1 ttgcttctcc aggaggattt tgcgctttat atcgccactg tgcccatctt cctgagttgg
       61 atacgcttcc ttctttttca acagacgatg ccattggcag taaaaatacc tcggtcttaa
      121 tggctttagg atttgcaccc gggcgcttcc agaaaataga tgtttcagtt tcccacaggt
      181 cagcacatac cagccagtta agcttttcca gcgccttagc ttctttcatt gaatttggtc
      241 caccaactac agggttggta cccattaaaa ttgctccttc tattttactg ccggcataca
      301 attcttcata aatgtgaacg tgtgagtgtg gttttgatgc ttttggcaac cagtcaaatc
      361 caaagtcgtt ttccctggtt gcattatcgc caaaccatgc tttcagtaag ttgatcatat
      421 attttggttt gttctgccac cagttaacac tcattggatc ctttgtggta ggggtgttat
      481 tagcaatcca tgccttaagg tcagtatcct tcttgggtcc aaataccttg ggagcgtcca
      541 gatatcccgg taatatatgc cacagtattg catggtcagt agagccctga acattagatt
      601 ctccacgcaa tgcattaatg ccaccaccag ccatacccat attacccatt aaaagctgca
      661 atattgcata cgcgcggata ttctgtacac caattgtatg ctgtgttgca cccattgcat
      721 acagtatcgt tgcaatccgg tcaggtttat gagtgctggt atacagttca gcaactttct
      781 taaatgtgtc aactggacag cctgtagctg cagaaacctt ttcaggtgta tagcgtgaca
      841 tgtgtttctt caaaagctgg tatacacact gaggattctg caatgattca tcctttaagg
      901 gtatgccatc agcatcaagc tgatatttcc atgtcttttt gtcatatttg cgttcagcag
      961 gattatatcc actgaagatt ccatcattaa aagcaaaacc gggatctatc agatatgctg
     1021 cattggtata cttaaccaca tattctttct ggataagatc attctgcaat gcatagttaa
     1081 tgataccgtt gataaataca atatcagtac cgggacgtat tggtgcatac aggtcagcca
     1141 gtgaagatgt acgggtaaac cgtggatcaa cactgataag cttgccacct ttatcacgtg
     1201 cccgctgaac ccacttaaat gatatgggat ggttttcagc agcattgctg ccaatgatca
     1261 tgacaacatc ggcatgctga atatcaatcc agtgatttgt cattgcacca cggccaaatg
     1321 aagccgccaa actggcgaca gttgagctgt gtcaaagccg tgcctgatgt tctaaccgtg
     1381 ttattcccag cagacgagca cactttgacc atgcatagca ctccacatta tccagtgctg
     1441 caccaccaaa tcctgctaag ccatcacaac ggtttactgt aatgccattt tccttctcta
     1501 gaaatgttct atcacgggtt tcttttactc gctgagctat cattttgata gcatcatccc
     1561 agcttaacgt ttcccactct tcactaaaag gtttacgata gtaaacttta tcaagacgcc
     1621 gttttaccgg atttactgct atctggtata acgacattcc ctttgaacaa agagcacctt
     1681 cgttaattgg atgatctgga tcgccttcgg catttattac tttgccatca cgcgatgata
     1741 caataatacc gcaaccaaca ccacagaacg ggcatatagt ggtggattcc tttgcccctc
     1801 ttaacttgtc agtggattta catcctgtta agaaggttac tccacccaaa aatgaacccg
     1861 ctgccgtgac tcctgttaat ttaaacaaat ctcttctggt aaccggcatg agtacctcct
     1921 gaaaaaataa acagtagttc ttcacaaaac atgcagcact ataaagttct gcctgataaa
     1981 caataacact attgtattaa attttggcgt gatcgtagcc tcccatataa tacaataata
     2041 gtataaacag acacatccct ttttacttaa aaagggtatc tgcctcttct ttccaaacac
     2101 gggaggcaaa aagatttctc tttttaccct ttcgaccctg cattcatagc gttatatgag
     2161 ccttagaaaa cagggttcga agagaccttg ttcattaaaa atttaatata atataattgt
     2221 caaacattat tgcgtatgtt attataaaaa actgtataag aaatcattgt aaagtattga
     2281 caaatacgtc accatcagga ctatagcttc aattgcagga aaacagcgga aaatttatta
     2341 tgccaatgat acatcataca gaacatcttg aacagcgtaa agaatcgctt ttgaataatt
     2401 ctatgttttc agaagacctt tttgctctat atcggtcact tttttcatta cagtttcaaa
     2461 gccttcaaca ccttgcctca atcattactg attcaaaact cattgttgat accacacagt
     2521 acccaatgat tcagcctcat acactacagt tcactgatga aataattgct gacataaaga
     2581 aaatgttcaa aaatatagct gcgcttatta cacagtatca aaccgcttac tcatttgata
     2641 cattgcttgc aaaccttgat gacaatcctg agctatacaa agacataatc acagcattat
     2701 tatcaaaaga cagtacattt gaggcaatag ctcataataa taaacttgat actgatgagc
     2761 tgatatttgt gacagtaaat gcatacaagc cattattcat tgctcttaaa gatgcagcat
     2821 tgcaggatat aacccaattc cctgattata gtgaaggctt gtgtcccttt tgtggatttt
     2881 tacccgatat ggctaaaatt gttgagccaa aaaacaacaa acgattcctt cactgtgccc
     2941 tgtgtgaatg cgaatgggaa tacaaacgcg ttaaatgtgt tgcgtgtgga aatgaaaaca
     3001 ccgatacact tggttattac agttatgagc ctgacgaaaa ataccgcttt gattactgca
     3061 acaaatgcaa cacatatata aaaacaatcc gcataaccaa acaacatgat gaatcacagt
     3121 ttgacctgac ggtagaaaat ctcattacca attttcttga tgcttcagca ctacagatgg
     3181 gatatacacg ccagtaatct atggaagcta tagaagcatg tatccttgca ggcggtaaaa
     3241 gcagccgttt tggtagcgac aaaaccttac acctgttcaa aggcaggcca ctgattgaac
     3301 atgttttaca ttcactcaaa ccgctgtttc aaacaatttc aattattgca aacgatactg
     3361 ataaatataa ctatctcaca atacctgtat atcctgatat aatacctggt cttggtccgc
     3421 ttggtggcat ctatactgca ctcaagtgtt cagcaacgga ttacattttt gtatgcgcag
     3481 ctgatatgcc tttcttaaat caggaattta ttgcttttat gctgcaaatc ccccatatgt
     3541 atgactgcat catcccctgc tgggcaggga atactgagcc actccatgca gtgtactcaa
     3601 aacaatgcat tccccatatt gaaaaactta tcaaagaaaa aaaatacaaa atcagcttcc
     3661 tttttgaaca ggtaaggatg cgctatatca tggatgatga actcatcttt tatgccgaag
     3721 acccttcaga acttttttat aacatcaata aacaaagcga cattccacct tcgtttcaat
     3781 aattccagcc caatattccc agtgctacta tattcatgat gataggtact gcatagatgc
     3841 atgatactgc aataatcttc tttttaattg ttgaaagccc aatcacgata atagcaaaga
     3901 caaagaaatg gaaataccca aacaaaaaga taagccacac acccttgaaa gaaagattcc
     3961 agtattcgta ttcccacact aaatggccac caatatta
//
LOCUS       DAOSZP010000142         3994 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_18250_length_3994_cov_2.913684, whole genome shotgun sequence.
ACCESSION   DAOSZP010000142 DAOSZP010000000
VERSION     DAOSZP010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3994)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3994)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3994
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18250_length_3994_cov_2.913684"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            89..1393
                     /locus_tag="PK348_04255"
     CDS             89..1393
                     /locus_tag="PK348_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXK65457.1"
                     /translation="MKLKTIINVHRIFITDGWNIKTMYIKRDILSDVLEHLEKKEITL
                     IVGPRQAGKTTLMKMVQQDVLKKGGKTIFLSLDFEKDSVHFVSQQALIQKLQLECGSQ
                     KAYIFIGEIQRKENAGLFLKGLYDMELPYKFIVSGSGSLELKEKIHESLIGRKRVFEL
                     STITLQEFLQYKTGYKYHNTIKEYCRLHKEEAGYLLNEYLNFGGYPRVVLENTIKEKT
                     NILNEIYTSYIEKDISYLMKVEKIQAFKHCIRLLAFQVGKCINGAELSSTLGIAIQTV
                     KNYLAYAEKTFVIIMVSPFYRNVRKELTKMPVVYFNDIGLLNFINGTLGTVDMFSGGF
                     VFQNLIFLLLKDRYKNTGASIHYWRTKDKAEVDFVINKGLEIVPVEVKYSALKNLSVS
                     RSLRNFINTYSPKEAYIVNLTLACETVINNTKVYCIPWFELL"
     gene            1418..>3994
                     /locus_tag="PK348_04260"
     CDS             1418..>3994
                     /locus_tag="PK348_04260"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HXK65458.1"
                     /translation="MKAEWGIYRYPILVYRLHAIRKHIYPKISDLDAFIAISDEPIPF
                     EKIHDIRFSSIKPGAKWGKAYTCAWFHFKGKIPQQYRHSTVVCCVDIGGEGLVVNEHG
                     EPVSAINSRITFMDYLQPTWDMRVIDLDENTKDSGIIDVWVDAGFNGKILQPFGKARF
                     KYAYLPLCRDDVKDFYFDYLTLAFAYCACDDEPIKNNIKLLLDKSYAAVKNFLPHNVA
                     EAKKIISKMYDLEQKPHEVFLTAVGQGHLDLAWLWPVRESKRKAVRTLINALQNSMQY
                     PEFVFGASQPQMFEWIKQSQPVLYDAIKKAVNTGAIELQGGMWVECDTNIPCGESLVR
                     QIYYGKKFFKEEFNCDVVTCWLPDAFGFNGNLPQILKKSGIDYFCTIKLAWNEVNKFP
                     YTTFNWVGIDGSSVIAHMPPEGDYTSGATPLCVKRIVENFLEKDIHNNALLVYGNGDG
                     GGGPTQTHIELIKRQQKLQWLPQVHFGKSIDYFQRLDAIQHKLPAYHGELYLEKHQGT
                     YTTQALSKYYNRRLECLLHDCEFLATYAYCNGFTYPHQFFETIWKEVLLYQFHDILPG
                     SSVKRVYDESVQRYRLLAHELQTMINTIVDYLSESGGISCINTTPFTRTEYSKGDSSW
                     YRCQMEGYSSAYMEPVDSHFSVAGGDSYIENELLRVEFAKEGHIISVFDKTHRKEICT
                     DYLNKLVLYTDKFRFFNAWDIDSNYHQKRKRELKLVDYETHTDGPQVLRRNYFRHGHT
                     TLTQDIILYDGKPYVEFSTECDYHETFTMLRADFVPSVYSDTVTCDIQFGNIARSTKN
                     STSIEKAQFEICAHKWVDVSDNDYGVSLINDCKYGHRVKDGIISLNLLRSPVYPDPYA
                     DRG"
BASE COUNT         1317 a          664 c          828 g         1185 t
ORIGIN      
        1 aattatcgtg ttaatttata agtatatcga ttcttataat aatactattg gtatactcca
       61 tgtacagagt aatgtaagct tctgaaaaat gaagctgaag acaattataa atgtgcatcg
      121 tatattcatt actgatggct ggaatattaa aactatgtat ataaaaaggg acatattgtc
      181 tgatgtactt gaacatttag aaaaaaaaga gataacctta attgttggtc cacgtcaggc
      241 gggtaaaacc acgctcatga aaatggttca gcaagatgta ttaaaaaaag gaggaaaaac
      301 aatattttta agtttagatt ttgaaaaaga tagtgttcac tttgtatcac aacaggcttt
      361 gattcaaaaa ttacaattag aatgtggatc tcaaaaagca tacatattta taggtgagat
      421 acagcggaaa gaaaatgcag ggctgttttt aaaaggcctc tatgacatgg agcttccata
      481 caagttcatt gtttcaggtt caggtagcct tgagttaaag gaaaagattc atgaatcgct
      541 tattgggcgt aaaagagtat ttgaattatc taccataaca cttcaagaat ttttacaata
      601 caaaacgggt tataaatatc acaataccat taaagaatat tgtagattgc ataaagaaga
      661 agcaggttat ttactcaatg agtatttgaa ttttggagga tatccccgtg ttgtgttaga
      721 aaatacaata aaagaaaaaa ccaatatact gaatgaaata tatacaagtt atattgaaaa
      781 agatatatca tatttaatga aagtagaaaa gattcaggca tttaaacact gcatacgttt
      841 actggctttt caggtgggaa aatgtataaa tggtgctgaa ttatcatcaa ccttaggtat
      901 tgctattcaa acagtaaaaa attatttagc gtatgctgaa aaaacatttg tcattatcat
      961 ggtttccccg ttttacagga atgttcgcaa ggagttaact aaaatgcccg tggtctattt
     1021 taatgacatt ggattattaa attttattaa tggtacattg ggtacagtgg atatgttctc
     1081 aggtggtttt gttttccaga atcttatatt tttgttattg aaagatagat ataaaaacac
     1141 aggcgcatca attcactact ggaggactaa ggacaaagct gaagtggatt ttgtgatcaa
     1201 taaaggattg gagatagttc ctgttgaagt taaatacagt gcactaaaaa atctcagcgt
     1261 tagcaggtct ttgcgaaatt ttatcaacac atattcacca aaagaggcat atattgttaa
     1321 cttaacatta gcttgtgaaa cagtaataaa taacacaaaa gtttactgta ttccctggtt
     1381 tgagttattg tagatatagc tttaccatga gaagagtatg aaagctgaat ggggcatata
     1441 tagatatcca attctggtat acaggttaca tgctatccga aagcatatct accctaaaat
     1501 ttcagatctt gatgcattca ttgctatcag tgatgagcca ataccctttg aaaaaatcca
     1561 tgacatacgc tttagcagca taaaacctgg cgcaaagtgg gggaaagcat atacctgtgc
     1621 ctggtttcat ttcaaaggta aaatccccca gcagtataga catagtactg ttgtatgctg
     1681 tgttgatatc ggcggagaag ggctggtagt caatgaacat ggtgagccag taagtgcaat
     1741 caacagccgt atcacattta tggattacct gcagccaacc tgggatatgc gtgttattga
     1801 ccttgacgaa aacacaaagg attcaggtat aattgatgta tgggttgatg caggatttaa
     1861 tggtaagata cttcagccat ttgggaaagc ccgtttcaag tatgcatacc ttccactgtg
     1921 ccgtgatgat gttaaagatt tttattttga ctacctgacg cttgcatttg cctattgtgc
     1981 ctgcgatgat gagcctataa aaaataacat caagctgtta ctggataaat cctatgcagc
     2041 ggtaaaaaat tttttaccac acaatgttgc agaagctaag aaaattattt caaagatgta
     2101 tgatcttgaa caaaaacccc atgaagtatt tcttactgca gtgggtcagg gacatctgga
     2161 tttagcgtgg ctgtggccag tgagagaatc aaagcgcaaa gctgtgcgta cactaatcaa
     2221 cgcacttcag aatagcatgc aatatcctga atttgtgttt ggtgcaagcc agccgcagat
     2281 gtttgagtgg ataaagcaaa gtcagcctgt actctatgat gcaataaaaa aagcagtaaa
     2341 cactggtgct attgaattgc agggtggcat gtgggttgag tgcgatacca atataccctg
     2401 cggtgaatcg ctggtgcgcc agatttacta tgggaaaaaa ttttttaaag aggaattcaa
     2461 ctgtgatgta gtaacctgct ggcttcctga tgcatttggt tttaacggta accttccgca
     2521 gatattaaaa aaatcaggca ttgactattt ctgtaccatt aagcttgcgt ggaatgaggt
     2581 caataaattt ccttacacaa catttaattg ggttggcatt gatggcagca gtgtcatagc
     2641 ccacatgcca cctgaaggtg attacacaag tggagcaaca ccgctgtgtg taaaacgcat
     2701 tgtagaaaat tttttagaaa aggatatcca taacaatgcc cttcttgtct atgggaatgg
     2761 cgatggtggt ggtggtccta cacaaacaca tattgaactg atcaagcggc agcaaaaact
     2821 gcaatggtta ccacaggtgc attttggcaa atcaatagat tattttcaac ggctggatgc
     2881 tattcaacat aagcttcctg catatcatgg tgaactgtac ttagaaaagc atcagggaac
     2941 ctacaccaca caggctttaa gtaaatatta caaccgtaga ttggagtgcc tgcttcacga
     3001 ttgtgaattt ctggcaacgt atgcatattg taacggcttt acatatcctc atcaattttt
     3061 tgaaactatc tggaaggagg ttttgctgta tcagtttcat gacatcttgc ctggttcaag
     3121 cgtcaaaagg gtttatgatg aaagtgtgca acgctacagg ctgctggcac atgaactgca
     3181 aacaatgatc aacaccatag ttgattatct ttcagagtct ggtggcatat catgtattaa
     3241 tacaacacct tttacccgta cagaatatag taagggcgat agttcatggt atcggtgcca
     3301 gatggaagga tattccagcg catacatgga acctgtcgat tcccattttt cggttgcagg
     3361 tggcgacagt tacattgaaa atgaactgtt gcgggttgaa tttgcaaaag aaggccatat
     3421 catttctgta tttgataaaa cacatagaaa agagatttgc acagattatc tgaataagct
     3481 ggtgctctac actgacaaat tcaggttttt caatgcgtgg gacattgaca gtaactatca
     3541 ccaaaaaaga aaaagagaac tgaaacttgt agattatgaa acacatactg atggtccaca
     3601 ggtgctcagg cgcaattact ttcgtcatgg ccacacaacc cttacacagg atattattct
     3661 gtatgatgga aagccttatg ttgaattttc cactgagtgt gattaccatg aaacgtttac
     3721 aatgctcaga gctgattttg taccatccgt gtacagcgat acagttacct gcgatattca
     3781 atttggcaat attgcacgaa gcaccaaaaa cagtacatca attgaaaaag cacagtttga
     3841 aatttgtgcg cacaaatggg tggatgtttc agataatgat tatggtgtat cgttaatcaa
     3901 cgattgcaaa tatggacaca gggttaagga tggtattatc agcctgaatc tattgcgttc
     3961 gccagtgtat cctgaccctt atgcagatag aggc
//
LOCUS       DAOSZP010000143         3987 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_18283_length_3987_cov_3.519329, whole genome shotgun sequence.
ACCESSION   DAOSZP010000143 DAOSZP010000000
VERSION     DAOSZP010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3987
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18283_length_3987_cov_3.519329"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1134
                     /locus_tag="PK348_04265"
     CDS             <1..1134
                     /locus_tag="PK348_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HXK65459.1"
                     /translation="KQKGYIYTRIMNPTQDVLEERVAMLEGGRAALALASGTSAIFYA
                     IINVCRAGDEFISASNLYGGTYTMFDAILPQFGITCKMVPYNDYDAYEKAISPKTRAI
                     FIESIGNPTLGVPDFEKVATIAQKYHVPLIVDNTFATPYHFRPIEYGANVVVHSLTKW
                     LGGHGTAIGGIVVDAGNFDWTDTKFALYNEPDAGYHGMRFAHDLGDMNSLAFILRMRL
                     VALRNLGAAISPDNAWIFLQGLETFPLRMQRHSENALKVAQFLQSHKNVAWVRYPGLV
                     GDSSYKVASRYFNNGFGGMVVFGIKGGYESAVKFINSIKLFSHLANVGDAKSLVLHPA
                     STSHSQLSEEQQREAGITPDLIRLSVGIEHSDDIIAALQEALE"
     gene            1495..2349
                     /locus_tag="PK348_04270"
     CDS             1495..2349
                     /locus_tag="PK348_04270"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HXK65460.1"
                     /translation="MVKKILIILLCLIVVLGAVYGYVYYKASKVPENNAQTMVKAALP
                     HDKKIVVCFGDSLTHATVSFDYVGFLAKDPDLQNYIFVNEGINSRLVYNLLQVVDTVV
                     ALKPHYVFVWIGTNDLKGSLNDKEYQRYNDLWNLPQKPTKEWFEQNYRQLVSILKTKT
                     DAKIVLISLPPLGENIDSLPFKLSMEYSAIIRDIAQKEKLGYIGLNEILTRDLIREGK
                     RDVAPYTTGLWFMYSAIIQHYLFGRDWDTISDSRGLTYMTDNIHINGKAGRILAAAIK
                     EKLISNKK"
     gene            complement(2410..3714)
                     /locus_tag="PK348_04275"
     CDS             complement(2410..3714)
                     /locus_tag="PK348_04275"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5982 domain-containing protein"
                     /protein_id="HXK65461.1"
                     /translation="MKKTVVCIIILCLFFALQNAMAARLSDEDLAKKIEGWYPTGLPL
                     VNYDSDNGFGYGVRVYLYNNGTRDDEYFAYAPYKMQLYGQFYQTTNGYQYHEINLDMP
                     YIFGTKFRIITSIAYDKKINANYFGQGADTAKGKLTLYTTYPTAYETFDKFQDYLDYA
                     DNDERFLKYYNYEYTKPSYFLNVYRDITQNLKFLAGLEVKKVTIDTWDGEKFDGTPQG
                     PTLLQQLQPLGYDGGWSNFVRAGIYYDTRDYEPDPKTGYLIDYAFEISDDTIGSDYDF
                     TRHTVQLQYYFPLLQSLTLALRAGYTDANGDTPFFEMGYFGFSLNRRTGLGNNRTLRG
                     YREQRFVGPTMTVANAELRWKFAEANPWGQNFQFKITVFYDVGNVYDKAADPFTDPRF
                     SDYHQGYGGGLVIAWNMATIVHFYYGMSKEDSSVSVDFSHTF"
     gene            complement(3777..>3987)
                     /locus_tag="PK348_04280"
     CDS             complement(3777..>3987)
                     /locus_tag="PK348_04280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65462.1"
                     /translation="KLVWLSDVPSAGDTSYAGYVIQGNTLYFSYYTSDIHHDYPWILG
                     MIKESPIMMGKLDLNVLEQLAYTKQ"
BASE COUNT         1203 a          762 c          843 g         1179 t
ORIGIN      
        1 aaacaaaaag gctacatcta tacccgcatc atgaatccaa cgcaggatgt actggaagaa
       61 cgtgtggcca tgcttgaagg gggcagggca gcccttgcac tggcatcagg aacatcggca
      121 atattttatg ccattattaa tgtatgcagg gcaggggatg aatttataag cgcaagcaat
      181 ctgtatggtg gtacctatac catgtttgat gctatactgc cgcagtttgg cattacctgt
      241 aaaatggtgc cctacaatga ttacgatgcc tacgaaaaag caatatcacc aaaaacgcgt
      301 gctatcttta ttgagtcaat tggcaaccca acgttgggcg tgcctgattt tgaaaaagtt
      361 gcaacaatag cacaaaagta tcatgtgcct cttatagtgg ataatacgtt tgcaacgccg
      421 tatcacttca ggcccattga gtacggggct aatgtagtgg tgcattcact taccaaatgg
      481 ttaggagggc atggcaccgc cattggaggg atagtggttg atgcgggtaa ctttgactgg
      541 actgacacaa agtttgcact ctacaacgaa cctgatgcag gttaccatgg catgcgtttt
      601 gcccatgatc tgggtgatat gaacagcctt gcgtttatac tcagaatgag gcttgttgcg
      661 ctgcgcaatc tgggtgcagc aatatcgcca gacaatgcat ggatattttt gcaggggctt
      721 gaaacatttc cactcaggat gcagcgacac agtgaaaatg cgcttaaagt tgcacaattt
      781 ttgcaaagcc ataaaaatgt agcctgggta cgctatccag gtttagtggg cgatagttcc
      841 tacaaagtag cttcccgcta ttttaacaat ggttttggtg ggatggtagt ctttggcatt
      901 aaaggtggat atgaaagcgc tgtgaaattt ataaattcaa ttaagctatt ttcccattta
      961 gcaaatgtgg gtgatgcaaa aagtctggtg ctgcatccgg caagcacctc gcattcacag
     1021 ctgtcagaag agcagcagcg agaagcaggc attacaccag atcttatacg cctgtcggtt
     1081 gggatagaac acagtgatga catcattgcg gcactacagg aagctttaga ataaaaggtt
     1141 tatcagtaat ccccataaaa tcatgattta tactacatac tatgtataat acatgaaatc
     1201 atgatagcta catgctgact gagtgtgtgg caattctctc agtatgctat cattgtgatt
     1261 cctcatatgg tatagcataa aaatcttcca gaaattatta ccattgcgta tatacatgtg
     1321 ctactcagtg atttgatttt gatttgacat agttggtttt aaatggtaac ctaatataat
     1381 aaattatatt ttctgcatag atagtgaacg taatgattgc tataggatat ataattcagc
     1441 tacaaatcaa tacagttcac ttcaaaaaga tattgtaata attggaggaa aggtatggtt
     1501 aaaaaaattt tgattatact gctgtgcctc attgttgtgc ttggtgcagt gtatgggtat
     1561 gtgtattata aggcatcgaa ggttcctgaa aataatgcac agactatggt gaaagctgca
     1621 ctgcctcacg ataaaaagat tgtggtatgt tttggcgata gcttaacaca tgcaacagtg
     1681 agttttgatt atgttggttt cctggctaaa gatcctgatt tgcaaaacta tatatttgta
     1741 aacgagggga tcaattcacg gttggtgtat aatcttcttc aggtggttga cactgttgtt
     1801 gcactgaaac cacactatgt gtttgtatgg attggaacca atgaccttaa aggctcgctt
     1861 aatgataaag aataccagcg ttacaatgat ctgtggaatt taccacaaaa gccaacaaaa
     1921 gaatggtttg agcaaaacta tcgtcaatta gtcagtatat tgaaaacaaa gacagatgca
     1981 aagattgtgc tgatatcgct gccaccgctg ggcgagaata ttgacagcct tccatttaaa
     2041 cttagtatgg agtactctgc cattatcagg gatattgctc aaaaagaaaa attggggtat
     2101 ataggcttaa atgagattct tacccgcgat ttgatacgtg aaggcaaacg cgatgttgcc
     2161 ccatatacta ctggattgtg gtttatgtac agcgcaatta tacagcatta tctttttggc
     2221 agggattggg atacaatttc tgatagccgc ggtcttacct atatgaccga taacatccat
     2281 ataaacggca aggctggaag aattttagct gctgccatta aggaaaaact tattagcaat
     2341 aaaaaataaa gcctttaatc aggaaggcat tgttatatac agccttcctg attatcagta
     2401 actatcgccc taaaaggtat ggctgaaatc aacagatacg gaagagtctt ccttgctcat
     2461 tccatagtag aaatgtacaa tcgtggccat attccatgca ataaccagcc cgccaccata
     2521 accctgatgg taatcgctga aacgtggatc agtgaatgga tctgctgctt tgtcgtaaac
     2581 attgcccaca tcataaaata cggttatctt aaactggaaa ttctgccccc agggatttgc
     2641 ttcagcaaat ttccagcgca gttctgcgtt tgcaacagtc atggttggac caacaaaacg
     2701 ctgctcgcga taaccacgca gagtccgatt gttaccaaga cctgtgcgac gattgagtga
     2761 aaatccaaaa taacccattt caaagaatgg ggtgtcacca ttagcatcag tgtagcctgc
     2821 gcgcaatgct agcgttaatg attgcagtag tggaaaataa tattgaagct ggacagtgtg
     2881 gcgtgtgaag tcataatccg aacctatggt atcatcagat atttcaaacg catagtcaat
     2941 gaggtatcct gttttggggt caggttcata atcacgggta tcatagtaga tgcctgcacg
     3001 gacaaagttt gaccatccgc catcataccc taatggctgc agttgttgta ggagtgttgg
     3061 cccctgagga gtgccgtcaa acttttctcc atcccaggtg tcaatggtca ctttctttac
     3121 ttcaaggcca gcaagaaatt taaggttctg agttatatcc ctgtaaacat tcagaaaata
     3181 gcttggcttg gtgtattcat aattgtagta ctttaagaaa cgttcatcgt tatcagcata
     3241 atctaaataa tcctggaatt tatcaaatgt ttcatatgct gttggatacg tagtgtaaag
     3301 cgttaatttt ccttttgcag tatcagcacc ctgcccaaag taattggcat ttatcttttt
     3361 gtcatacgct attgaagtaa tgattctgaa tttagtgcca aagatatagg gcatgtccaa
     3421 attaatttcg tgatactgat acccattggt tgtttgataa aactgtccgt acaactgcat
     3481 cttatacggt gcataggcaa aatattcgtc atcgcgtgtg ccgttattat acagataaac
     3541 acgtacaccg tatccaaacc cattatcgct atcataatta accagtggta atcctgttgg
     3601 ataccagcct tcaatctttt ttgccaggtc ttcatcagag agacgggcag ccattgcatt
     3661 ttgtaatgca aaaaacaaac acaagataat aatgcatact acagtttttt tcatagtgcc
     3721 tccagtaata aaataatata acggcctggt ataaatctta catttaaaaa ttgatattat
     3781 tgtttcgtgt atgctaattg ttcaagtaca ttcagatcaa gcttgcccat catgatagga
     3841 ctttctttta tcattcccag tatccatgga taatcatggt gtatatcact ggtataataa
     3901 ctaaaataca gtgtgttgcc ttgtatcaca tatcctgcat aactggtatc gcctgcactg
     3961 ggcacatctg aaagccatac aagtttt
//
LOCUS       DAOSZP010000144         3981 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_18323_length_3981_cov_2.879012, whole genome shotgun sequence.
ACCESSION   DAOSZP010000144 DAOSZP010000000
VERSION     DAOSZP010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3981)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3981)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3981
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18323_length_3981_cov_2.879012"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..720
                     /locus_tag="PK348_04285"
     CDS             <1..720
                     /locus_tag="PK348_04285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65463.1"
                     /translation="DAIDTKKYKNLTVLQMKQSVGGHLKDFYFNYIDYGQRLDIFFGD
                     TPRITTKDVEWINREYDSILGKAKDYRGAIVHLIYSIVEYPDVAKDTWLPHRMKAIKR
                     GEYKGKHKYFVGFDTFEARIGNSAAIIKHHSIDPIVEYQIIDFFYGLRKALSPNIFSK
                     ILYHLWKTKNFDVIRQVKQRCLEEEKFYRAVIDVIERVYKLDLSNFGGYIYHIRNNAA
                     HWENDIDSPKDLKAFRAHMEK"
     gene            919..1125
                     /locus_tag="PK348_04290"
     CDS             919..1125
                     /locus_tag="PK348_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65464.1"
                     /translation="MIDNNSLIEKMMQKNFNRCCELTQAMFSLKHALYKSLFPTLSDE
                     EIWSKITEEILKRNHNHEKPTINS"
     gene            1100..1507
                     /locus_tag="PK348_04295"
     CDS             1100..1507
                     /locus_tag="PK348_04295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65465.1"
                     /translation="MKNQPSIHEYIITISSCFINTNIPFCIAGGIAVSLWGHVRATED
                     LDVIALIDETHDFLKRCIQNRKLFSVKGINIPVINLEDLIVLTCASGRYQDIADVQAL
                     LAGSFPIDRDYSIKQLDALQVTLAPDIQKIILQ"
     gene            1652..>3981
                     /locus_tag="PK348_04300"
     CDS             1652..>3981
                     /locus_tag="PK348_04300"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HXK65466.1"
                     /translation="MKQKPIHTVLSITLVICALAFLSCWDTTQTIDQDEKQPQINLRA
                     SVLEIPSSGTYDFGQIEVGQTKSAVITIENLGQKFLELTGPITLSGVNASLFDIENQP
                     NTTIPPFTYTTFTISFTPDSQGIKTATITITSNDPQKTTFTFTIQGEGIVITAPEINI
                     QYSGSNVNKGDSIHLGSCVIGNSSDFTLSIQNIGTADLTIQAPIISGIDQSLFAITTA
                     PANTVAAGSSTDFVVRFTPTSAGNKTAIITITNNDADEGTYSFTITASATTVPQPDIF
                     VMQDATPLYNTIGIFDFGQVNQGQTSSSVTFTIKNTGTANLTLTTPFTISGTDASMFS
                     VSPPGSTTIVPNDITSFSMTFAPSSAGEKNATVSIANDVPGKNPYTFSIKGTGIAIPE
                     IDIDQSGTPIANNGTFDIGSTTVNVSKDTLFTIYNNGLGILNLPTTPAVTLTGSPFFT
                     LVSYPGNTISPSSSSNFTLRYLPTEAGNHSTILTITNNDLDENPYIITITGQATSVPT
                     PKLEITKSSQVLLNNSSVDFGGMTVGGTPIDVTFTITNKGDSNLSITGTTISPGDFSI
                     VTAPTTPVTPGGTTTIIVRFLATSAGTKVAALMIGSDDLNNNPFTINLTGKGLEPEIN
                     CKINGSDFSSGSTYDFGNLAVGHSKDIVITIENLSSSDPLYLTGSPIVTKVSGGEFSV
                     ITQPSKVSIPPSGSTTFTIRFSPTVTGNQSAQFSISNSDSDENPYIINVQGNGTNATI
                     QVDEVASGGTIDYGNVKCNTGGLAHTFTIRNNGSTD"
BASE COUNT         1334 a          777 c          667 g         1203 t
ORIGIN      
        1 gatgccatag atactaaaaa atacaaaaac ctcacagtgc tgcagatgaa acaatcagtt
       61 ggcggacatt taaaagattt ttatttcaac tacattgact atggccagcg ccttgatatt
      121 ttttttggtg ataccccgcg catcactaca aaggatgttg agtggattaa ccgtgaatat
      181 gatagcattt taggaaaggc aaaagattac cggggggcga tagttcattt aatttacagc
      241 attgtggaat atccggatgt tgcaaaagac acatggcttc cgcaccgtat gaaagcaatc
      301 aaacgcggcg aatataaggg caaacacaaa tactttgttg gctttgatac gtttgaagca
      361 cgcattggca actctgctgc tattatcaaa catcactcaa ttgatccaat agttgaatac
      421 cagattattg actttttcta cggattacga aaggcactat cgcccaatat cttttccaaa
      481 atactgtacc atctatggaa aacaaaaaat tttgatgtca tacgacaggt aaagcaacgc
      541 tgtcttgagg aagaaaaatt ttaccgtgct gttattgatg ttattgaacg ggtctacaaa
      601 ctggatttat ctaactttgg cggatatatc tatcatatca ggaacaatgc tgcccactgg
      661 gaaaacgata ttgacagccc caaggactta aaagcattca gagcacatat ggaaaagtga
      721 tgctttttta caattgacaa cacgtgcttt gcaagatata tttcaatcat aaaacatata
      781 cacaacaatg tactggttgg tgaataagct gttgggtacc atttataact ttggtatgct
      841 ctttgcaaaa tttaatcaca cataaatcct tttttaagga agggtaagcc ttttagtgta
      901 tagaatggat tatgatgtat gatagataat aatagtttaa ttgaaaaaat gatgcaaaaa
      961 aacttcaacc gctgctgtga actcacccag gccatgtttt cattgaaaca tgcactgtat
     1021 aaatcattat ttcccactct cagtgatgag gaaatatggt ccaaaattac agaagaaata
     1081 cttaaaagaa accataatca tgaaaaacca accatcaatt catgaataca ttattactat
     1141 aagctcgtgt tttattaata ccaatatccc tttctgcatt gctggaggta ttgctgtcag
     1201 cttatggggt catgtaagag caactgaaga tttagatgtt attgctttaa ttgatgaaac
     1261 acatgatttt ttgaaacgct gtattcaaaa tcgcaaacta ttttcagtga agggaatcaa
     1321 cattcctgtt attaatcttg aagatcttat tgtgcttaca tgtgcttccg gcaggtacca
     1381 ggacattgcc gatgtacagg cattgttagc tggttctttt cccattgaca gggactatag
     1441 tataaaacag ctggatgcat tacaggtaac tttagccccg gatatacaaa agattatttt
     1501 gcaataacta cattctggaa ggaaaacatt atgtcatata acctatcact ttatttattc
     1561 aaaaaataat ttaatttttt atattttaca aaaatttttt atttcacaat atcaagtaat
     1621 tattgtacta tgtatatagc aattattgtg tatgaaacaa aaacctatac atactgtttt
     1681 aagcataacg ttagttatat gtgctttagc ttttttatcc tgctgggata cgacgcaaac
     1741 tattgaccag gacgaaaagc agcctcaaat aaacctacgt gccagtgtgc ttgaaatacc
     1801 ttcatcagga acctatgatt ttggacaaat agaagttggt caaactaagt cagctgtaat
     1861 aactattgaa aacttaggtc aaaaattttt agaacttaca ggtcccataa cattaagtgg
     1921 ggtaaatgcc tctttatttg atattgaaaa ccagccaaac actaccattc caccttttac
     1981 ctatacaact tttactattt cttttacccc tgattcacag gggataaaaa cagccacaat
     2041 aacaattaca agcaatgatc ctcaaaaaac tacgtttact tttactatac aaggtgaagg
     2101 gattgttatc actgcgcctg agattaacat tcaatatagt ggaagcaatg taaacaaagg
     2161 tgattctata catttgggta gttgtgtaat agggaattca tccgatttta ctctttccat
     2221 tcaaaatatt ggcacagctg acctcaccat acaggcacca attataagtg gaattgatca
     2281 aagtctattt gctataacca ctgctcctgc caatactgta gcagcaggtt cttcaacaga
     2341 ttttgtggtc agatttactc caacatccgc tggtaacaaa actgctatta ttaccattac
     2401 caataatgat gccgatgaag gcacatatag ctttacaata acagcttcag caacaacagt
     2461 tccccagcct gacatctttg tcatgcaaga cgctactccc ctgtataaca ccattggtat
     2521 atttgatttt ggccaagtta atcaaggaca aactagctct tcagtaacat ttaccatcaa
     2581 aaatactggt actgcaaatc ttacattaac tactcctttt actatatcag gaactgatgc
     2641 ttcaatgttt tctgtttcac ctccaggatc aacaaccatt gtaccaaatg atataacatc
     2701 attttcaatg acgtttgcgc cgtcttcagc aggtgaaaaa aatgcaacag tttccattgc
     2761 taatgacgtg ccaggaaaaa atccatatac cttttcaata aaaggtactg gtatagctat
     2821 accagaaatt gatatagatc aaagtggaac cccaattgcc aataatggaa cttttgacat
     2881 tggctcaaca actgttaatg tttctaaaga tactctattt accatataca ataatggcct
     2941 gggtattctt aatcttccaa ccacccctgc tgtaacctta accggttccc ctttttttac
     3001 acttgttagc tatccaggca atacaattag tccatcatct tcatcaaatt ttacgttacg
     3061 ctatcttcct actgaagccg gcaaccattc aacaatattg acaattacca acaatgatct
     3121 tgatgaaaac ccatacataa tcacaattac cgggcaggca acgtctgttc caacaccaaa
     3181 acttgaaatc accaaatcat cacaggtgtt attgaacaat agttctgttg attttggcgg
     3241 catgaccgtt ggtggaacac caatagatgt tacctttacc ataactaata agggcgatag
     3301 taacctttca ataactggca caacaatttc gcctggtgat ttttcaatag taactgcccc
     3361 aacaactcct gtaacaccag gtggaacaac aacaatcatt gtaagatttt tagctactag
     3421 cgcaggcaca aaagtagctg ctcttatgat aggaagtgat gatctaaata ataacccttt
     3481 tactattaac cttacaggta aaggtcttga acctgaaatt aattgcaaaa taaatggctc
     3541 tgattttagc agtggcagca cgtatgactt tggcaatctt gcagtgggac atagcaaaga
     3601 tattgttatt actattgaaa atctttcatc aagcgacccc ctgtatttga caggaagtcc
     3661 aatagttaca aaggtttcag gtggtgagtt ttcagttata actcagccat caaaagtaag
     3721 tattccacca tctggatcta caacgttcac aatacggttt tcaccaaccg ttacaggcaa
     3781 tcaatcagca caattcagta tatcaaacag tgatagcgat gaaaacccat acattataaa
     3841 tgttcaggga aatggtacaa atgctaccat tcaagttgat gaagtagcca gtggtgggac
     3901 aattgattac ggcaatgtta aatgtaacac aggaggttta gcacatacat ttaccatacg
     3961 caataatggt agtactgatt t
//
LOCUS       DAOSZP010000145         3980 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_18329_length_3980_cov_3.091465, whole genome shotgun sequence.
ACCESSION   DAOSZP010000145 DAOSZP010000000
VERSION     DAOSZP010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3980)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3980)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3980
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18329_length_3980_cov_3.091465"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            406..606
                     /locus_tag="PK348_04305"
     CDS             406..606
                     /locus_tag="PK348_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65467.1"
                     /translation="MQITISKNELYTMIKKAVKEVIYEERLDILLKSIPAISDEEMTE
                     IKNLYGHKPPKRKAAKRITIDI"
     gene            complement(890..1672)
                     /locus_tag="PK348_04310"
     CDS             complement(890..1672)
                     /locus_tag="PK348_04310"
                     /inference="COORDINATES: protein motif:HMM:NF014037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mut7-C RNAse domain-containing protein"
                     /protein_id="HXK65468.1"
                     /translation="MKEYTVTIRFYEELNDFLPKDKKKVAFTVSWEGKRSIKDLIESL
                     GVPHVEVDLIQVNGISVGFDYIVQNDDYISVYPVFERFEISNITRLRPQSLRAPAFVC
                     DVHLRKLVKYLRLFGFDVLYNETWDDKDLAHIAAEQKRILLTRDRQLLMRKIVDRGLI
                     VRNTSPQKQIVEILDRLDLWDKINPFSRCIMCNGNVTELSNNTEEFEKEKNRIPPGVL
                     SWCNTYYKCTQCGRIYWEGSHYEKLTKKIETIINQKSVDKDS"
     gene            complement(1669..2577)
                     /locus_tag="PK348_04315"
     CDS             complement(1669..2577)
                     /locus_tag="PK348_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HXK65469.1"
                     /translation="MDNGKHYEAIVAKKVTWTGMIVNIILSGLKFVAGILGHSQAMVA
                     DAIHSISDLTTDIAVLVGIKYWASPADDDHPYGHERIESIVTAFIALALFAVGIGIAY
                     NGIITAFEPDSKGPEAIALIAAFISILSKEWLYRWTVKKGKEIDSSSVIANAWHHRSD
                     ALSSIPALVAVALAIAHPSLVFFDHIGAVVVAMFIVKVAFDILKPVVMELSDRGASKS
                     QVDAIDLIAMKVDGVKEVHSIRSRRVGSGYFVDLHVLVDGNMSVQDGHDIARSVKHEL
                     LDKGPKVLDVIVHLEPYQTSSKKNNS"
     gene            complement(2570..>3980)
                     /locus_tag="PK348_04320"
     CDS             complement(2570..>3980)
                     /locus_tag="PK348_04320"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HXK65470.1"
                     /translation="HDPVSDAGIGFYPSPSIHSITIGYITTHTVKYALLGLVLFILTF
                     LLGRFFCGWMCPLGATIDASDTVIQPKGKTKVQTNLSRIKFLVLLSSMLIAFFGFTVS
                     GYIDPITIAYRAYELFLYPFVSFIGSKPLSWILPGYQARDYPFYYYNWAWVAVVFFVV
                     LGLSAFFRRFWCRVLCPLGAMYAIASHVSLFRRVVDTDKCIHCGACVKGCRMGAIATD
                     GVGTIEHECIKCFDCLKNCKYDAISFTFTRPSKNKSIEYPGKGITRRDLLIATGSSVI
                     VAASKVSLFAASHDSFIIRPPGALRESQFLDACIRCGQCMNVCPTNALHPCITEAGFY
                     GMFTPRLISRIGYCDFTCTRCGQVCPTGAIQKLSLNSKQKFVIGTAVILHDICLPWSE
                     QTNCIVCEEVCPVANKAITLEKKIVKNKKGLQIEVLLPVVHEDRCIGCGICENRCPVS
                     GQAAIVVRKPKLSIGERYG"
BASE COUNT         1318 a          807 c          631 g         1224 t
ORIGIN      
        1 atcataccct ttctgtgaca gatatgcagc aatagaaaca agaccaacag gtagcaatgt
       61 aatataataa tcatgactgt gttctttttc cacacaatag ccaagtacaa tcttcatata
      121 gcccctttat tattttatcg cagggaatac aatcattctt gaatattaac atagcttctt
      181 aattatattt tactattatg actgttatga ataatttgac caaaaattta ctaaactctg
      241 tgtaatttct atgtaaaccc cttatttggt ggtattttta taataaatat gagataaaaa
      301 tattttacat taatttattt tacctgcatt attgatatct taatttaata cttagccaca
      361 tataccagta aaaattataa tttttataat tttatatagg ggaatatgca aattactatc
      421 tcaaaaaacg aactctatac aatgattaaa aaggcggtga aagaagttat ttatgaagaa
      481 cgacttgata tattattaaa aagtatacct gccatttcag atgaagaaat gactgaaata
      541 aaaaaccttt atgggcataa gccaccaaaa cgtaaagctg ctaaacgaat aacaattgat
      601 atatgaaatg gaccatagaa tattccaaaa atactgttag ttttcttaaa aataatcaat
      661 ctattgaaga taatattata aacgaaataa aaaggtaatc caaaaattac agggtcaaac
      721 tgttaatatc aatttaaaaa agctttctgg tgaatgggaa ggatactatc gtattcgcaa
      781 aggtaaaata cgtattatta tttatcttga ttttttaaaa agaactgttt atgtagaaaa
      841 tgttgatttt cgaggaaaca tatataaata atcatgtttt taaaactatt taagaatctt
      901 tatctactga tttctggttt attatcgttt caattttttt tgttaatttt tcgtagtggc
      961 ttccttccca gtatattctg ccacattgtg tgcatttata atatgtatta caccagctca
     1021 gtacacctgg cggaattcga tttttttcct tttcaaattc ttcagtattg ttagacaact
     1081 cggtaacatt cccattacac attatgcatc ggctgaatgg atttatctta tcccataaat
     1141 ccagcctgtc taatatttct actatttgct tctgtggact ggtattgcga acaattaatc
     1201 ccctatcaac aatctttctc atcagtaact gtcggtcacg agtaagcaaa atacgctttt
     1261 gctctgctgc tatgtgggct aaatctttat catcccaggt ttcattatat aagacatcaa
     1321 aaccaaataa acgaagatat ttaacaagtt tacgtaaatg gacatcgcag acaaatgcag
     1381 gtgcacgcag actttgcggc cttaaacggg taatattact gatttcaaat cgttcaaaaa
     1441 cagggtatac gctgatataa tcatcattct gaacaatata atcaaatcca accgaaatcc
     1501 catttacctg tataagatca acttcaacat ggggaacacc cagggattct attaaatctt
     1561 ttatactgcg tttgccctcc cagcttacag tgaatgcaac cttcttttta tcttttggca
     1621 ggaagtcatt gagttcttcg taaaaacgaa ttgttacagt atattctttc aactgttatt
     1681 cttttttgaa gaagtttgat atggctccag gtgtacaata acatccagaa cttttgggcc
     1741 tttatctaaa agctcatgct ttacgctgcg ggcaatatca tgtccatcct gtaccgacat
     1801 attgccatca accagtacat gcagatcaac aaaatatcca ctaccaacac ggcgtgaacg
     1861 aatgctgtgc acctctttga ccccatcaac cttcattgca attaaatcta ttgcatccac
     1921 ctgcgattta gaagctcctc tgtcagatag ttccatcact acaggtttta aaatatcaaa
     1981 tgcaacctta acaataaaca tagcaacaac aacagcacca atatggtcaa agaaaactaa
     2041 agaaggatgt gcaatggcaa gtgctaccgc aactagcgct ggtatggaac tcaatgcatc
     2101 tgagcggtga tgccatgcat tggcaataac cgaagaagaa tcaatttctt ttcctttttt
     2161 tactgtccat cgatacagcc attctttaga tagaatagaa ataaaggcag ctataagtgc
     2221 aattgcttca ggacctttac tgtcaggctc aaatgccgtt attataccat tgtacgcaat
     2281 gccaatgcca acagcaaaaa gggctaatgc aataaatgct gtaacaatac tttctatgcg
     2341 ttcatgacca taagggtgat cgtcatcagc agggcttgcc cagtatttga tgcctacaag
     2401 tactgcaata tctgtcgtta aatcagaaat actatgtata gcatcggcaa ccattgcctg
     2461 actgtgtccc agtatccctg caacaaattt caatcctgac agaatgatat tgactatcat
     2521 tccagtccag gtaacttttt tagctacaat ggcttcatag tgttttccat tatccataac
     2581 gctccccaat acttagcttt ggtttacgaa ccacaatagc tgcctgccca cttaccgggc
     2641 acctgttttc acagatacca catccaatac atctgtcttc atgaacaact ggcaataaaa
     2701 cttcaatctg tagtcctttc ttatttttga caatcttttt ttctaatgtt atagccttgt
     2761 tagcaaccgg gcatacttct tcacatacaa tgcaatttgt ttgttctgac catggtaagc
     2821 atatatcatg caggataaca gcagtgccaa taacaaactt ttgtttactg ttaagcgata
     2881 gcttctgaat tgctcctgtg gggcaaacct gtccacagcg tgtacaggta aaatcacagt
     2941 acccaatgcg cgatattaag cgtggcgtga acattccata aaatccagct tcagtaatgc
     3001 atggatgaag cgcattggtg ggacacacat tcatacattg cccacaccga atacatgcat
     3061 ctaaaaattg tgattcgcgc aatgcacctg gtggacgaat gatgaagcta tcgtgtgatg
     3121 ctgcaaaaag cgatacttta ctggcagcaa ctatcaccga tgaccccgtt gcaatgagca
     3181 agtctcttcg agttattcct ttgccaggat actcaatact tttgtttttc gatggtctgg
     3241 taaatgtaaa ggaaatagca tcatacttac agtttttcag gcaatcaaaa catttaatac
     3301 actcatgctc tattgtgcca accccgtcgg tagctatcgc ccccatacga cagcctttaa
     3361 cacatgcccc acaatgaata catttgtctg tatcaacaac ccgtcggaat aatgaaacat
     3421 gtgaagctat agcatacatt gcacccaatg ggcataacac cctgcaccag aaccgcctga
     3481 aaaatgcaga taaccctaac acaacaaaga aaactacagc aacccatgcc cagttgtagt
     3541 aataaaacgg ataatcccgt gcctgatatc caggcaatat ccatgacaga ggctttgaac
     3601 ctatgaatga tacaaacgga tataagaaaa gttcataagc acgataggca atggtaattg
     3661 gatcaatata tcctgacact gtaaaaccaa agaatgctat aagcattgaa cttaacaata
     3721 ctaaaaattt gattctgctt aagtttgttt gtacttttgt ttttcctttt ggttgtataa
     3781 cagtatcaga agcatcaatg gttgcaccta acggacacat ccatccgcaa aaaaaccttc
     3841 caagcaaaaa agtcaaaata aataacacta accctaacaa tgcatacttg actgtatgag
     3901 ttgtaatgta tccaattgtt atactgtgta ttgaaggtga aggataaaat cctattcctg
     3961 catcagaaac tggatcatgt
//
LOCUS       DAOSZP010000146         3971 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_18382_length_3971_cov_3.802094, whole genome shotgun sequence.
ACCESSION   DAOSZP010000146 DAOSZP010000000
VERSION     DAOSZP010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3971)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3971)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3971
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18382_length_3971_cov_3.802094"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="PK348_04325"
     CDS             <1..288
                     /locus_tag="PK348_04325"
                     /inference="COORDINATES: protein motif:HMM:NF014460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXK65471.1"
                     /translation="LGSIGGGIVAYFQLDVSFDVYYTHILDSLHFKATYVGLFKALVF
                     GMMVSTISCANGLRATNGALGVGKATRNSVVSSFLMVLIVGYYITALFYGK"
     gene            318..1064
                     /locus_tag="PK348_04330"
     CDS             318..1064
                     /locus_tag="PK348_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002763919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXK65472.1"
                     /translation="MIEFRNVTKILSGTKVLNNLSFKIEKGETFVIIGRSGTGKTVTL
                     KHIAGLLEPDSGQILVGGEQMNGISKSQRDRLREKIGFVFQSGALINWMTVEENIALP
                     LIEHKLYPPDEIKKIVEEKMDLLQLTSARDKMPAEISGGMKKRASLARVLVRNPEIIL
                     YDEPTSGLDPVMSSLINTIINQLKKDYTITQVVVTHDMDSAYAVGDRIAMLYDGRIIQ
                     CDEPDGIRNTDNPIVRQFISGSLEGPIDVL"
     gene            1089..1742
                     /locus_tag="PK348_04335"
     CDS             1089..1742
                     /locus_tag="PK348_04335"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HXK65473.1"
                     /translation="MKQEIAVGIIVIIALTVLGYFTIIMSGEIFETHTYYPMTVVFKD
                     VEGLSADDKVRVNGVLSGYVDTVELIENNVIVTLRLYNHFTLYENYEIMVRNETALAG
                     KYVSISPGTPMDENGKQYAVITTRDNLIGTSVPDPFTMLSRLIADNRGNVNATVKNLR
                     DITDKINSGKGTLGKIINEDTVHGQATDLIKQLQDTIEDTREQAPITSFLRAALTAF"
     gene            1909..>3971
                     /gene="carB"
                     /locus_tag="PK348_04340"
     CDS             1909..>3971
                     /gene="carB"
                     /locus_tag="PK348_04340"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008593663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HXK65474.1"
                     /translation="MPRRTDIKKILIVGSGPIVIGQACEFDYSGSQACKALREEGYEI
                     VLVNSNPATIMTDPDMADKTYIEPITHDIVARIIEKERPDALLPTVGGQTALNIAVQL
                     HESGVLQQYGVELIGAKIDAIKKAEDRDLFKKAMLDIGLQVPNSALASSIDEALKILE
                     TIPLPIIIRPAFTLGGTGGNIVYNQEDFVELVRKGLDESPISQVLLEESVIGWKEFEL
                     EVMRDLKDNVVIICSIENFDPMGIHTGDSITIAPQQTLTDKEYQNLRDMAIKIIREIG
                     VETGGSNIQFAVNPKDGRVMVIEMNPRVSRSSALASKATGFPIAKIAAKLAVGLTLDE
                     IANDITRETPACFEPTIDYVVTKIPRWAFEKFEGADTTLGTQMKSVGEAMAIGRTFKE
                     SFQKALRSLEIDRYGFGSDGNLKLDALVDAIPERQRNDIIEQNLRIPREQRVFYIKKA
                     LELDWSVEKIHELTSIDPWFLHQLKELVEAEREFVAQAKQGLQKENILKMKQLGYSDR
                     QLAFLIHKDELKALYERGLDAQKDYSKRLNELEDSIRKFRFEQAIVPGYRLVDTCGGE
                     FEAYTPYYYSSYDDEDEAKHTTKRKVMILGGGPNRIGQGIEFDYCCCHASFALREAGI
                     ESIMVNSNPETVSTDYDTSDRLYFEPLTLEDILHIYNNEKPDGVIVQFGGQTPLRLAR
                     RLEANG"
BASE COUNT         1226 a          667 c          922 g         1156 t
ORIGIN      
        1 ctgggcagca ttggtggtgg tattgttgca tactttcagc ttgatgtaag ttttgatgta
       61 tattataccc acattcttga ttcattgcat ttcaaggcta cctatgttgg gcttttcaaa
      121 gcgcttgtat ttggcatgat ggtttctacc atcagctgtg ctaatggact cagggcaacc
      181 aatggagcgt tgggtgttgg taaggcaaca agaaattcag ttgtatcatc tttccttatg
      241 gtacttattg tggggtacta tattacagct cttttttatg gaaaataaaa ttctatagcc
      301 atcgaaatgt atacattatg attgaattta gaaatgtaac caaaatactc agtgggacaa
      361 aggttctcaa taacttgagt ttcaaaatag aaaaaggtga aacgtttgtt atcattgggc
      421 gttcaggtac gggtaaaaca gtaacattaa agcatattgc aggattgctt gagcccgata
      481 gcgggcagat tttagttggt ggtgaacaaa tgaacggtat cagcaaaagc cagcgagata
      541 gattgcggga aaaaattgga tttgtttttc aatcaggtgc gctgattaac tggatgactg
      601 ttgaggaaaa tatagccctt ccactcattg agcataaatt atacccacca gatgaaatta
      661 aaaagatagt agaggagaag atggatttat tgcaactcac atcagcccgg gataagatgc
      721 ctgctgaaat aagtggtggg atgaaaaaac gtgcaagtct ggcacgggtt cttgttcgga
      781 atccagaaat cattttgtac gatgagccaa catctggact ggatccggtg atgtcaagtt
      841 tgatcaatac tataataaat caattgaaaa aagattatac aataacacag gttgttgtta
      901 ctcatgatat ggatagtgct tatgcagttg gtgacagaat tgcgatgctg tatgatggga
      961 gaattattca atgtgatgag cctgatggaa tacggaacac cgataacccc attgtgcggc
     1021 agtttatttc aggttccttg gaaggtccta ttgatgtgtt atgattataa aattatttgg
     1081 agggtgatat gaaacaggag atagcagttg ggataatagt tattatagct cttacggtgt
     1141 tgggctattt tactattata atgagtggtg aaatctttga aactcataca tattacccaa
     1201 tgacagttgt gtttaaagat gttgaaggtt tatcagcgga tgataaagtg cgggttaatg
     1261 gggtattgtc gggatacgtt gatacggtag aattaataga gaataatgta attgtaacgt
     1321 tgcggttata taaccatttt acgctgtatg aaaattatga aattatggta cgcaatgaaa
     1381 ctgctcttgc tggtaagtat gtaagcatca gtccaggaac accaatggat gaaaatggca
     1441 aacaatatgc ggttataacc accagggata accttattgg gacatcagtg cctgaccctt
     1501 ttaccatgct ttcgcgtctt attgcggata acaggggaaa tgttaatgcc actgttaaga
     1561 atttgcgtga tataactgat aaaattaatt cagggaaagg aacgcttggc aaaataatta
     1621 atgaagatac cgttcatggg caggcaactg atttaatcaa acagttgcag gatactattg
     1681 aagatacccg tgaacaggca ccaatcacaa gcttcctgcg tgctgcatta acagcttttt
     1741 aatactattg ttacaatcac gttaaatttt tcactaccaa taaaaaaatt attggaattt
     1801 ttcttgcacc aaactcattg ttctgaagtt ttaatggtta ttttaataaa ttttgataca
     1861 ttttgataaa atgtatgtat acaccactac atagaaagga atgtttgcat gcccaggcgc
     1921 actgatatta aaaaaatttt gatagttgga tcaggaccca ttgttatagg gcaggcgtgt
     1981 gaatttgatt attcaggttc acaggcctgt aaagctttgc gtgaagaagg ttatgagata
     2041 gttctggtaa attcaaaccc tgctaccata atgaccgacc ctgatatggc tgacaaaaca
     2101 tatattgaac caataaccca tgatattgta gcacgcatta ttgaaaagga aagacctgat
     2161 gcattgttac ctaccgttgg tgggcagact gccctgaata ttgctgtcca gcttcatgaa
     2221 agcggagtat tacaacaata cggtgttgaa ctgattggtg caaagattga tgccataaaa
     2281 aaagctgaag accgggatct ctttaaaaag gctatgcttg atattgggct tcaggtgcca
     2341 aattcggcgc tggcatcttc aattgatgaa gctttgaaaa tactggaaac cattccactt
     2401 cccattatta tcaggccagc gtttactttg ggtggtacag gtggcaatat tgtatataat
     2461 caggaagact ttgttgagct ggtacgaaaa gggcttgatg aatcgcccat aagtcaggta
     2521 ttactggagg aatctgtcat tgggtggaaa gagtttgagt tagaggtcat gcgtgacctc
     2581 aaagataatg tggtcattat atgttccatt gaaaattttg atcctatggg cattcacact
     2641 ggcgattcca taactatcgc cccccagcaa accctgaccg ataaagaata tcaaaattta
     2701 agggatatgg ctatcaagat aatccgtgag attggtgtgg aaaccggcgg ttcaaatatt
     2761 cagtttgcgg ttaatcccaa agatggcagg gtgatggtta ttgagatgaa tccccgtgtc
     2821 agccgaagtt ctgcattggc gtcaaaggca acgggctttc ccattgcaaa gattgctgca
     2881 aagttggctg tggggcttac ccttgatgaa atagcaaacg atattacccg tgaaacgcca
     2941 gcatgctttg aacctaccat tgattacgtt gttaccaaga ttccacgctg ggcatttgaa
     3001 aagtttgaag gtgctgacac aacgcttggc acccagatga aatcggtggg cgaggcaatg
     3061 gcaattggtc gtaccttcaa agaatcgttt cagaaagcat tgcgttctct ggagattgac
     3121 cgctatgggt ttggttcgga tggcaattta aaacttgatg cacttgttga tgcaatacct
     3181 gagcggcaac gaaatgacat cattgaacag aatttgcgta ttccgaggga acaaagagtc
     3241 ttttatataa agaaagcatt ggagcttgat tggagtgttg aaaaaattca tgaattaaca
     3301 tcaatagacc catggttttt acatcaactc aaagaactgg ttgaagcaga gcgtgaattt
     3361 gttgcacagg ctaaacaggg tttgcaaaaa gaaaacatac tcaaaatgaa acaattgggt
     3421 tactctgaca ggcagcttgc atttttaatc cataaagatg aactcaaggc attgtatgaa
     3481 cgggggcttg atgcacagaa ggattattca aagcgactga atgaacttga agacagtata
     3541 aggaagttta gatttgagca ggcgatagtt cctggatacc ggctggttga cacctgcggt
     3601 ggtgaatttg aagcctacac cccctattac tattcttcgt atgatgatga ggatgaagct
     3661 aagcacacaa caaaacgcaa ggtcatgata ttgggtggtg gcccaaaccg tattgggcag
     3721 ggcatagagt ttgactactg ctgctgtcat gcttcttttg cattgcgtga agctggtata
     3781 gagtcaataa tggtaaattc aaatcctgag acagtttcaa ccgattatga tacttcggac
     3841 agattgtatt ttgagccatt gacactggaa gatatactcc atatttacaa caatgaaaaa
     3901 cctgatggcg ttatagtaca gtttggagga caaactccct tgcggcttgc acgccgactt
     3961 gaagcaaatg g
//
LOCUS       DAOSZP010000147         3956 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_18470_length_3956_cov_2.751858, whole genome shotgun sequence.
ACCESSION   DAOSZP010000147 DAOSZP010000000
VERSION     DAOSZP010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3956
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18470_length_3956_cov_2.751858"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..652)
                     /locus_tag="PK348_04345"
     CDS             complement(<1..652)
                     /locus_tag="PK348_04345"
                     /inference="COORDINATES: protein motif:HMM:NF015084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HXK65475.1"
                     /translation="MNTWRLIIHNACAPSWNMAVDSAILRLTKIPTLRLYRWSTPAIT
                     IGYFQDIEKEVHIELCKKDDIPVIRRLTGGGAVFHHEELTYSLVHPLQGPFGKSSITH
                     SYALIAQPLIHALRSMGVDAQYRPINDIIVGNRKISGSAQTRKEGKLLQHGTILVATD
                     TEKMLQYLTIDPKKIKQEGNPVITLSEIIKGLSKESMFEQLIKAVISSFKELFAVDF"
     gene            complement(649..933)
                     /locus_tag="PK348_04350"
     CDS             complement(649..933)
                     /locus_tag="PK348_04350"
                     /inference="COORDINATES: protein motif:HMM:NF021921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate protein ligase C-terminal
                     domain-containing protein"
                     /protein_id="HXK65476.1"
                     /translation="MSIHVVLKNPGEKLVHIDIDFEKSTISWIHISGDFFIYPEEAIG
                     DIEQALLGLPVAPQVIEQTIFTLIKNNDIEMVGISVSTIVQGIQEAFAQS"
     gene            complement(930..1781)
                     /gene="lipA"
                     /locus_tag="PK348_04355"
     CDS             complement(930..1781)
                     /gene="lipA"
                     /locus_tag="PK348_04355"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004071207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HXK65477.1"
                     /translation="MGRVRTIPDWIKFSIPSGEQYKQVQKHVAGCGLHTVCLEARCPN
                     IGECFSKGHATFLIMGNICTRNCKYCAVTKGQPQPLNIHEPQNIARAVANLNLEYVVI
                     TSVTRDDLEDGGAFHFVKTIEAIRTAIPDTKIEVLIPDFKFSQQRSLTMIMDAHPDVI
                     NHNIEVVKSYYHTLRPKGDYQASLTVLAIVTEHGFIAKSGLMIGFGETMDDIINTLED
                     VYSAGCRVLTVGQYCASHRGAYPVHKYYTPEEFKEIETIAYQIGFMKVLAAPLVRSSY
                     RAGELFS"
     gene            complement(1769..3193)
                     /gene="gcvPB"
                     /locus_tag="PK348_04360"
     CDS             complement(1769..3193)
                     /gene="gcvPB"
                     /locus_tag="PK348_04360"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013239063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HXK65478.1"
                     /translation="MQTIFSLHKKGRNKYTVPQFGAESYDPIPQHLCRGELNLADVSE
                     VDVVRHYRRLSSLNFGVDNGMYPLGSCTMKYNPKINDAVASLPQFTQQHPATIEELSQ
                     GSLHIMYSLQEMLCAITGMKQFTLMPAAGAHGELTSVMIIKKYFETKGEKRNIILIPD
                     SAHGTNPASVAMCGFTVKEIPSNSDGDVDIDALRAAMDNNVAAMMLTCPNTLGLFDRN
                     IMTIADILHSNGALFYCDGANLNAIVGKVRPADLGFDIMHVNLHKTFSTPHGGGGPGS
                     GPIGVVEKLTPFMPVPVIAKKDNQYYLDYERSHSIGRVHSYYGNFLVMLRAYTYILML
                     GASGLKQVAEHAVVNANYLRVKLQKHFNLPYTRICMHEFVINDEGMPGGITTNEIAKR
                     LLDYGFHAPTVYFPLIVHGAMMIEPTETEHRDTLDAFVDTMIAIKNEANTNPEVLKAA
                     PQTTPVRKVDAVLAARKPVLKWEE"
     gene            complement(3205..>3956)
                     /locus_tag="PK348_04365"
     CDS             complement(3205..>3956)
                     /locus_tag="PK348_04365"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF014408.2"
                     /note="acts in conjunction with GvcH to form
                     H-protein-S-aminomethyldihydrolipoyllysine from glycine;
                     forms a heterodimer with subunit 2 to form the P protein;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycine dehydrogenase"
                     /protein_id="HXK65479.1"
                     /translation="ELSHIINDTVACVVVQSPNFFGVIEDIEKVAHIAHEHKALCIAT
                     FTEAMSLGLLKSPGECGADIACGEGQSFGNYPANGGPLLGILACKKEFMRTIPGRLIG
                     KTVDEDGRECYVMTLQTREQHIRRERATSNICTNEGLCALRATIYLGLVGNRLVEIAR
                     TNHTAASYCLEQLVNLGYERVFDASFFNEFVIKINNAKDVQNKLMDNGFALGIPLETY
                     YPELSDCLLVTATECTTKDDIDTLVKLLQTI"
BASE COUNT         1115 a          870 c          737 g         1234 t
ORIGIN      
        1 caaaatcaac tgcaaaaagt tccttaaacg aagaaatgac agccttaatt aattgctcaa
       61 acatactttc ttttgacaaa ccttttatga tttcactcaa cgtgataacg ggattccctt
      121 cctgttttat ttttttagga tcgatagtta aatactgtag catcttttct gtatctgttg
      181 ccacaaggat ggttccatgt tgcaacaatt tcccctcttt tcgggtttgt gcactgcctg
      241 agatttttct attgccaaca ataatgtcat tgatggggcg atactgtgca tcaactccca
      301 tactacgcag tgcatgaatc agtggctgtg caatcaatgc ataagaatgg gtaatactac
      361 ttttgccaaa tggtccctgt aaagggtgca ccagactata ggtaagctct tcatgatgaa
      421 acactgctcc accgccagta agcctgcgaa taaccggaat atcatctttt ttgcaaagtt
      481 ctatatgtac ctctttttct atatcctgaa aataccctat agtaattgca ggcgtactcc
      541 accggtacag ccgaagcgtg ggtatttttg taagcctcaa tatggcacta tctactgcca
      601 tattccatga tggtgcacat gcattgtgaa taatcaggcg ccaggtattc atgattgtgc
      661 aaaagcctcc tgtatgccct gtacaatcgt ggaaaccgaa attccaacca tttctatatc
      721 attattttta atgagtgtaa aaatagtttg ttcaattacc tgtggagcaa ctggtaagcc
      781 caacaaagct tgttctatat cgccaattgc ttcttcagga tatataaaaa aatcacctga
      841 aatgtgaatc catgaaatag tactcttttc aaaatcaata tcaatatgga ctaatttttc
      901 accaggattt ttaagtacaa catgaatgct catgaaaaaa gctcacctgc tctgtatgaa
      961 ctgcgtacaa gcggtgctgc aagtactttc ataaaaccta tctgataggc aatggtttca
     1021 atttctttga attcttctgg tgtataatat ttatgtactg gatatgcccc cctgtgcgaa
     1081 gcacagtact gcccaactgt cagtaccctg cacccggcgg aatatacgtc ttcaagcgta
     1141 ttgataatgt catccatggt ttccccaaat ccaatcataa gacctgactt ggcaataaat
     1201 ccatgctcgg taactatcgc caaaacagta agcgaagcct gataatcccc ttttggacgt
     1261 aatgtatggt aatagctttt tactacttca atgttgtgat taatgacatc agggtgagca
     1321 tccattatca ttgtaagtga acgttgctga gaaaatttaa aatcagggat taatacctca
     1381 atttttgtgt caggaatagc agtgcgtatg gcttctattg tttttacaaa atgaaatgct
     1441 ccgccatcct caaggtcatc acgcgtcacc gatgtaataa ccacatattc taaattcagg
     1501 tttgccacag ctctggctat gttttgaggc tcatgtatat tcagtggttg cggttgccct
     1561 ttggtaacgg cacaatattt acagttacgc gtgcagatgt tgcccatgat aagaaatgtt
     1621 gcatgccctt tagaaaagca ttctccaata ttgggacagc gtgcctcaag gcacacagta
     1681 tgcagaccac agcctgctac atgtttttgc acctgtttat actgctcacc tgatggtatt
     1741 gaaaatttta tccagtcagg tatggtcctt actcttccca tttcagtacc ggtttgcgcg
     1801 ctgcaagcac tgcatcaact ttgcgcactg gcgttgtttg cggtgcagct ttcagcactt
     1861 ctggatttgt gtttgcttca ttttttattg caatcatggt atccacaaat gcatcaagcg
     1921 tatccctgtg ttcagtttcc gttggttcaa tcatcattgc accatgaaca atgagcggga
     1981 agtaaaccgt tggtgcatgg aaaccataat caagaaggcg cttggcaatt tcatttgttg
     2041 ttataccacc tggcatgcct tcgtcgttta tgacaaactc atgcatacat atacgtgtat
     2101 acggcaagtt aaaatgcttt tgcaatttaa cacgcaagta gtttgcattg acaacggcat
     2161 gttcagccac ctgtttcaag ccactggctc ctaacatcaa tatgtaggta taggctctga
     2221 gcattactaa aaaatttcca taatagctat gcactcgccc aattgaatgt gacctttcat
     2281 aatcaagata atactgatta tccttttttg caatcacagg cactggcata aagggagtaa
     2341 gtttttcaac aacaccaatt ggtcctgaac ctggtccgcc accgccatgt ggtgttgaaa
     2401 aggtcttgtg caaattaaca tgcatgatat caaaacctaa atctgcaggg cgcactttgc
     2461 caacaattgc atttaagttt gcaccatcac agtaaaacaa tgcaccatta ctgtgtaata
     2521 tatctgcaat tgtcataata tttctatcaa aaagccctaa ggtattggga caggtcagca
     2581 tcatagctgc aacattgtta tccattgcag cacgcagtgc gtctatatcg acatcaccat
     2641 cgctgttgga aggaatttct tttacagtga acccacacat ggcaactgat gcaggatttg
     2701 taccatgtgc tgaatcaggg atgaggatga tattccgttt ttcacctttt gtttcaaaat
     2761 atttctttat aatcataaca cttgtaagct ctccatgtgc accagctgca ggcataaggg
     2821 taaactgttt cattcctgtt attgcacata gcatctcctg caaggaatac attatatgta
     2881 acgatccctg tgaaagctct tcaatagttg ccgggtgttg ctgagtaaac tgaggtaaac
     2941 ttgcaactgc atcgtttatc tttggattat atttcatggt gcagcttccc aacggataca
     3001 tgccattatc aacaccaaaa tttaagcttg aaagcctacg gtaatggcga acgacatcaa
     3061 cttcgctcac atcagcaagg ttaagctcac cacggcatag gtgttgtggg ataggatcat
     3121 agctttctgc accaaattgc ggtacagtat atttattcct gcctttttta tgtaatgaaa
     3181 atattgtttg catggtactc accattatat tgtctgcaat agttttacaa gtgtgtctat
     3241 atcatcttta gtagtacatt cagttgctgt aaccaaaagg caatcagata gctcaggata
     3301 gtatgtttcc agcggtatcc ctaatgcaaa tccattatcc ataagcttgt tttgaacatc
     3361 ttttgcattg ttaatcttaa tgacaaattc attaaaaaat gacgcatcaa acacacgctc
     3421 atagccaaga ttaactaatt gttccaggca atagcttgct gcagtatggt ttgtacgagc
     3481 aatctctact aacctgttac ctaccaggcc aaggtatatt gttgcccgca gggcacacaa
     3541 tccttcattg gtacatatat ttgatgtggc gcgttccctg cgtatatgct gttctcgtgt
     3601 ttgcagtgtc atcacataac attccctacc atcctcatca accgttttgc caataagcct
     3661 tccaggaatg gttcgcataa attctttttt gcaagcaagt atccctaaca aaggccctcc
     3721 attggcaggg taattgccaa acgactgccc ttcgccgcat gcaatatcgg caccacattc
     3781 accagggctt tttaatagcc ctaaactcat tgcttcagta aatgttgcaa tgcataatgc
     3841 tttgtgctca tgtgcaatgt gtgctacttt ttcaatgtct tcaatcacgc caaagaagtt
     3901 gggactttgc acaacaacgc aggcaacagt atcgtttatt atgtgcgata gttcat
//
LOCUS       DAOSZP010000148         3955 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_18474_length_3955_cov_3.018718, whole genome shotgun sequence.
ACCESSION   DAOSZP010000148 DAOSZP010000000
VERSION     DAOSZP010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3955)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3955)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3955
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18474_length_3955_cov_3.018718"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1300
                     /locus_tag="PK348_04370"
     CDS             <1..1300
                     /locus_tag="PK348_04370"
                     /inference="COORDINATES: protein motif:HMM:NF012935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HXK65480.1"
                     /translation="LVSDVIVIGSGPGGATVARELSHAGKKVIILDKGADHRGRIYYG
                     TYPGAMIYSDKMSLLFTKEGLNIISPVMVGGATSMYCGCAAPPPVWLKKRYRIDIDKE
                     VNEAIQELNIQPLPPQLRGIASTRIAQAASQLGYDWFPQPKFMNPERSKHFHCTAACM
                     LGCRCGAKWNAAEWVDEALQSGAALYTHVKIKKILHDGRSVQGVEGTIDGKKFIAHAP
                     YVVVAAGGIGSPRLLQASGFKDAGKGMTMDVTVMVYGTISDKGIGKEPPMTWSWENDA
                     DGYMLSTLIDPWLLYPLAAMRVGVKPLLQWFQWNHILGVMIKLKDDIAGGVYSDGTIS
                     KPLTHKDKERLAKARAVCEAILKKAGAPASSIFMRPLIGTHPSGTVRIGDMLDTNLQT
                     KIDGLYVCDASTFPESLDRPTVLTIIGLGKRLAKHLIKKR"
     gene            1310..2659
                     /locus_tag="PK348_04375"
     CDS             1310..2659
                     /locus_tag="PK348_04375"
                     /inference="COORDINATES: protein motif:HMM:NF012544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HXK65481.1"
                     /translation="MNGLKRVLGLPAVTFIAVGFTIGGGVFVFTGIVLKMVGPALPLA
                     YLLAVVPVFLSMLPLAMLGSAIPSVGGNYKYPSRMVSPGIAFVGIWIYVLATFFGQIP
                     LYSISCARYISTFYPELSLKICAIALVTLFYIVNLIGIKPAATVQAIMVIVLLSALLF
                     FSFSGIARFDVANFANFFEKGAGNLMVGVALLTFTYLGSNGIIELGGEIQNPGTTIPR
                     AYFITFPVVTAVYFCVALATAGSMPWQKTAQEAEPLIFLSRSFLSNAGVMFFIIGGAV
                     LALTTTLNALFIVGTKSLLIIIQDKLLPSWLGSIHQTFGTPHILLSVIWLLSIVGIVS
                     DLSIETFASYSALGGIIIFLPILIAALKFPRLYPEKYQKAAFKLTGAWLHVCVIVGFV
                     LILFFCLVILIDLKTMFKIGLFIVFVLSGVIYYFLRKRYLAKQGIHLEAITQKEDWG"
     gene            2663..3733
                     /locus_tag="PK348_04380"
     CDS             2663..3733
                     /locus_tag="PK348_04380"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HXK65482.1"
                     /translation="MSELSLYKVFPDLQKLPHVSLGNFPTPVQRLTSLGHDSLWIKRD
                     DVSSALYGGNKVRKLEFTLAEAIVTGKKKVVTMGGIGTNHGLATAIFCKHLGLGCRLL
                     LFWQPVTEYVKRNLLLFVRYGAEIHYYKTMLKTGFMFFTKERLAHDAYLLYAGGSSPL
                     GTVGFVNAAFELKSQIEKNELPEPDYIVCALGSAGTMAGLWLGAKLAGLKSTVVGVRV
                     TDRSLGPVPIANEKSVLSLITKTYTLMKSVTPLPPINVTTPVILHDWCGEGYGYPTGA
                     CLDTIETMKTSEHIQLEPTYTGKTFAAVCDMIQKKEYADKTILYWHTYNSVDLSQEVK
                     KANYHDLPQSLHWVFEDYNSLQ"
     gene            3754..>3955
                     /locus_tag="PK348_04385"
     CDS             3754..>3955
                     /locus_tag="PK348_04385"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HXK65483.1"
                     /translation="MRYFFIMEHGTTIIERLNQFFKSRNNVEKVLLFGSYARNTQTKR
                     SDIDIIIITETNKRFFDRYDDFN"
BASE COUNT         1102 a          751 c          947 g         1155 t
ORIGIN      
        1 acttgtttca gatgttatag tgattggatc gggccctggt ggggcaacag ttgctcggga
       61 actatcgcat gctgggaaaa aagtcattat attggataaa ggagctgatc accgcgggcg
      121 catttactat gggacgtatc ctggtgcaat gatttattct gataagatga gcctgctttt
      181 tacgaaagaa gggcttaata tcataagccc agttatggtt ggcggtgcaa ccagtatgta
      241 ctgtggttgc gcagcgccac cgcctgtatg gctcaaaaaa cgctatcgaa ttgacattga
      301 taaagaagtc aatgaagcaa ttcaggaact gaatatacag cccctgccac cacagctaag
      361 gggaatagct tcaacgcgga ttgctcaggc tgccagtcaa ctgggatatg actggtttcc
      421 acagccaaaa tttatgaatc ctgaacgtag caaacatttc cattgtactg cagcgtgtat
      481 gctgggatgc aggtgtggtg caaagtggaa cgctgccgaa tgggtggatg aagcgttaca
      541 gtcaggagca gcattatata cacatgtaaa gataaaaaag atactccacg atggaagatc
      601 agtgcaggga gttgaaggca ctattgacgg gaaaaaattt attgctcatg ctccgtatgt
      661 agtagttgca gcaggaggta ttggaagccc acgcttgtta caggcttctg gatttaagga
      721 tgcaggtaaa ggcatgacca tggatgttac tgttatggta tatggtacaa tcagtgataa
      781 aggtattggc aaagaaccgc caatgacatg gtcatgggaa aatgatgcag atggctacat
      841 gctctcaacg cttattgacc catggttgtt gtatccgctg gcggcaatgc gtgtgggggt
      901 taaaccattg ttacagtggt ttcagtggaa ccatattctt ggggtaatga ttaaacttaa
      961 agacgatata gccggaggag tttattctga cggaactatc tccaaaccat taacgcacaa
     1021 agacaaagaa cgcctggcaa aagcacgtgc ggtgtgtgaa gcaatactta aaaaagcagg
     1081 tgctcctgct tcatcaattt tcatgcgccc attaattggc actcacccca gcgggaccgt
     1141 gcgcattggc gatatgcttg ataccaatct tcagacaaag attgatggcc tgtatgtttg
     1201 tgatgcaagc acgttccctg aatcactgga ccgtcctaca gtacttacca tcattggatt
     1261 aggcaagcgc ctagcaaagc accttattaa aaaacggtag aggtgcggta tgaatgggct
     1321 aaaacgggta cttgggcttc cggcagtaac ctttattgct gttgggttta ccattggggg
     1381 tggcgttttt gtattcaccg gtatagtatt gaaaatggtg gggcctgcct tgccgcttgc
     1441 ataccttctg gctgttgttc ctgtattttt aagcatgctg ccactggcaa tgttaggttc
     1501 agccattcca tcggtgggtg gcaattataa gtatccaagc cgaatggtct caccaggcat
     1561 tgcatttgtg ggtatatgga tatatgtgct ggcaacattt tttgggcaaa ttcccttata
     1621 cagcatttcg tgtgcacgct acatcagcac cttttacccg gagctatcgc tcaaaatatg
     1681 tgcgatagct cttgtgacat tgttctatat tgtgaacctc attggcatta agccggcagc
     1741 aaccgtgcag gccatcatgg tgatagtgtt gctcagtgcg ttgctgtttt tttcgttcag
     1801 tggtattgca cggtttgatg ttgcaaactt tgccaatttt tttgaaaagg gagcaggtaa
     1861 ccttatggtg ggggtggcat tgttgacctt tacgtattta ggatcaaatg gtatcattga
     1921 attaggtggt gaaatacaga atcctggcac cacaattccc cgagcgtact ttatcacatt
     1981 tcctgttgtc accgcagtgt atttttgtgt ggctttggca actgctggta gtatgccctg
     2041 gcaaaaaaca gcacaggaag cagaaccttt gatcttttta agcaggtcgt ttttaagcaa
     2101 tgctggtgtc atgtttttta ttataggtgg cgcagtgctg gcacttacca caacactcaa
     2161 tgccctgttt atcgttggga caaaatcgct gttaataatt atacaggaca aattgttacc
     2221 atcctggctt ggaagcatcc atcaaacatt tggaacgcca cacatattac ttagtgtgat
     2281 atggctgttg tccattgtgg gaatagtttc tgatctttcc atagaaacat ttgcgtcata
     2341 ttcagcactg ggtggcataa ttatttttct gccaattctc atagcagcac taaagtttcc
     2401 gcgattgtat ccggaaaaat accagaaggc agcattcaag cttacaggtg catggctgca
     2461 tgtatgtgtt attgttggtt ttgtgctgat acttttcttt tgccttgtga tacttattga
     2521 ccttaaaacc atgttcaaga ttggattgtt tattgtattt gttttaagcg gtgtcatcta
     2581 ctattttttg cgaaagcgct atttagcaaa acaggggatt catcttgaag caattacaca
     2641 aaaggaagat tgggggtaag ctgtgagtga attgtcatta tataaagtat ttcctgactt
     2701 acaaaaacta ccccacgtat cattgggaaa ttttcccaca cctgttcaaa ggcttacatc
     2761 attggggcac gatagtttat ggattaaacg cgatgatgta tcatcggcgc tgtatggggg
     2821 aaacaaggtg cggaaattgg aatttacatt agcggaggcg atagtaaccg ggaaaaagaa
     2881 agttgtgaca atgggaggca ttggcaccaa tcatgggctg gcaaccgcta tcttctgcaa
     2941 acacctgggg cttggatgca ggttgcttct attctggcaa cctgtgacag aatatgtaaa
     3001 aagaaatctg cttttatttg tacgctatgg tgcagagata cactattaca aaacaatgct
     3061 gaaaacaggc tttatgtttt tcacaaagga acggttagcg cacgatgcat acttattata
     3121 tgctggcggt tcttcgccat tggggacagt gggatttgtt aatgctgcct ttgaactaaa
     3181 aagccagata gaaaaaaatg agttgcctga gcctgattat attgtgtgtg cattgggttc
     3241 cgctggtacc atggctggct tgtggttggg tgcaaaacta gcgggtttaa aaagcactgt
     3301 ggtaggagtc agggtaaccg acagatcact ggggcctgtg ccaatagcca atgaaaaaag
     3361 tgtcctgtca ttgattacaa aaacatatac tttgatgaag agcgtcacac ctttgccacc
     3421 cattaatgtt acaacgcctg ttatattgca tgactggtgt ggtgaaggct atggatatcc
     3481 aaccggcgca tgcctggata ctatcgaaac aatgaaaacg agtgagcata ttcaacttga
     3541 accaacctat accggcaaaa cctttgcagc cgtttgtgat atgattcaga aaaaagaata
     3601 tgctgataaa accatactgt actggcatac ctacaactca gtagatttat cacaggaagt
     3661 taaaaaggcc aattaccacg atttaccaca aagcttacac tgggtgtttg aagattataa
     3721 tagcttacaa taagtattga catgatactg taaatgcgct acttttttat catggaacat
     3781 ggtactacca ttattgagcg tttgaatcag ttttttaaaa gtaggaacaa tgttgaaaag
     3841 gtgctgttgt ttggttccta tgctcgcaat acgcaaacaa agcgcagtga tattgatatt
     3901 atcataataa ctgaaaccaa taaacgtttt tttgatcgct atgatgattt taatg
//
LOCUS       DAOSZP010000149         3949 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_18512_length_3949_cov_3.450437, whole genome shotgun sequence.
ACCESSION   DAOSZP010000149 DAOSZP010000000
VERSION     DAOSZP010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3949
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18512_length_3949_cov_3.450437"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..774)
                     /locus_tag="PK348_04390"
     CDS             complement(<1..774)
                     /locus_tag="PK348_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002685639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HXK65484.1"
                     /translation="MSLYQKVYDESINNPEKFWSEAARNIHWYKPFTKVLDDSRKPFY
                     RWFVGGQVNTCYNALDRHVEGGKGNQVALIYDSPVTDTIAKFTYKEMLDKVSIFAAAL
                     QKYGIKKGDRVIIYMPMIPEAPVAMLACARIGAIHSVVFGGFAPNELAIRIDDAKPKL
                     IVSASCGIEGKKVIEYKPLLDKAIEIANHKPEHCIIFQRPQVTSPLMPGRDIDWNDAI
                     IGVKPAECVPVESTDPLYILYTSGTTGIPKGVVRDNGGHA"
     gene            complement(1018..1470)
                     /locus_tag="PK348_04395"
     CDS             complement(1018..1470)
                     /locus_tag="PK348_04395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65485.1"
                     /translation="MNIETTTCIAHENLKLLQDYADIYKLSLHTFIINFINYVMSYKK
                     IPVKSCKRLTYRKRYESWKRVHLYLYENEYEFLMDARKVCKMSIAKVISYCIENYLFD
                     FLAALDSEDNTDNYRFSGYTFMFYLENGIQCCRFYWGPHPELVKYAMQ"
     gene            1663..1914
                     /locus_tag="PK348_04400"
     CDS             1663..1914
                     /locus_tag="PK348_04400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65486.1"
                     /translation="MISTPVTIATHNRSGAGYNEVIAVVRFADGPGREMQVKTQAQVN
                     GSYGFTVSGANVYDTQGRVIAQGKPYFEAGEALPIHQCQ"
     gene            complement(2075..2548)
                     /locus_tag="PK348_04405"
     CDS             complement(2075..2548)
                     /locus_tag="PK348_04405"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HXK65487.1"
                     /translation="MYSTKELIDIAIGIEETGFYFYSTFKNKFKDESFKQIFQFLADE
                     ELRHKGVFSDMLKELKDTQGLFTEEYYSYLKAIGTTRVFANKDDIDKIAPSLHQPLDI
                     IKIAMDAERDSIVWYSELKEIYRNDAKAKNILERLINEERKHVLTLLDLKEKLIM"
     gene            complement(2594..>3269)
                     /locus_tag="PK348_04410"
     CDS             complement(2594..>3269)
                     /locus_tag="PK348_04410"
                     /inference="COORDINATES: protein motif:HMM:NF013079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M17 family metallopeptidase"
                     /protein_id="HXK65488.1"
                     /translation="KPSGHIETMRTDMAGAAACLYTINAIAQMKLPINVYAVIPLAEN
                     MISHTAYKPGDVFTAYNGKTVEIGNTDAEGRLILADALSFTEKKLKPKAIIDIATLTG
                     ACIVSFGEIVAAYLTSDESLSTALFNASESTGEKIWRMPLYKEYNEEIKSDIADIVNV
                     PAVRNAGTIIGATFLKNFIENTPWAHIDIAGTSWYSKKRGYNPKNATGFGVRLLYEFA
                     KSVALK"
     assembly_gap    3270..3279
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3280..>3949)
                     /locus_tag="PK348_04415"
     CDS             complement(<3280..>3949)
                     /locus_tag="PK348_04415"
                     /inference="COORDINATES: protein motif:HMM:NF013079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M17 family peptidase N-terminal
                     domain-containing protein"
                     /protein_id="HXK65489.1"
                     /translation="IINTKTFKAKPNTYFFAALKDRPSILLVGLGSHKDITPESLRNA
                     ASVIVGACSDKSVSEVHIVVPEINVTNDTARYIAEGVYLANYAFNRYKTQKEETSPLL
                     EKAILLSDNPGQLSPLLKEVSIVCSNTLLCRDLVNEITENANPLTIAKLAKKIAAQSK
                     IKCTVYNKKDIEKMKMGLLLAVSQGSKYPPQLIVMHYRGAPSKKDSIALVGKGITFDT
                     GGIN"
BASE COUNT         1167 a          752 c          741 g         1279 t
ORIGIN      
        1 agcatgcccg ccattatccc gcacaacacc tttaggaatt ccagtggtgc cggaagtgta
       61 tagtatatat aatggatcgg ttgattcaac cggtacacat tcggcaggtt tcactccgat
      121 tatagcatca ttccaatcta tatctcttcc cggcataaga ggtgatgtga cctgagggcg
      181 ctggaaaata atgcaatgtt caggtttatg attggctatt tcaattgctt tgtcaagaag
      241 aggtttgtat tctataactt ttttaccttc aataccacat gaagcagata caataagctt
      301 tggtttagcg tcatcaattc gtattgctaa ttcatttggt gcaaatccac caaacaccac
      361 cgagtgaata gctcctattc gcgcacatgc gagcattgca acaggtgctt caggtatcat
      421 gggcatgtag ataataaccc ggtcaccttt tttaatccca tatttctgta aagcagcagc
      481 aaatattgaa accttatcaa gcatctcctt gtatgtaaac tttgcgatag tatccgtaac
      541 aggactatcg tatataagtg caacctgatt acccttacca ccttcaacat ggcggtcaag
      601 cgcattataa catgtgttga cctgtccacc tacaaaccat cgataaaaag gcttacggga
      661 atcatcaagt actttagtaa atggtttata ccagtgaata ttccttgcgg cctctgacca
      721 aaacttttcc ggattattaa tagattcatc atacactttt tgatacaaac tcataggctt
      781 ccaatccccc tttctatgga ttaaatagta tctgtttagt ttgaccagac caatgatttg
      841 gctggagatg taaaatattg gttgccaacc aaattatttg gctaaagtta tttgtcaatg
      901 ataaatatct gtagtattac aaaatttaga atttaagtaa tagagaggta gccccttgtt
      961 aactttggta ggaaaaaata aattttacgt tgcatgatct tataacaatg taagtggtta
     1021 ctgcatggcg tatttcacca attcagggtg tggtccccag taaaatcgac agcattgtat
     1081 accattttct aaataaaaca taaatgtgta gccactaaat cgatagttat cggtattatc
     1141 ctcactatca agtgctgcaa gaaaatcaaa aagatagttt tcaatacagt aggaaataac
     1201 cttggcaatg gacatcttgc acactttgcg cgcatccatc aaaaactcat actcattctc
     1261 atatagataa agatgcaccc gcttccagct ttcatagcgc tttcggtatg taagccgctt
     1321 gcagcttttc accggaatct ttttgtatga catcacatag ttaataaaat taataataaa
     1381 cgtgtgaagc gatagtttgt agatatcagc ataatcctgt agcagtttta aattttcatg
     1441 ggcaatgcag gtggtagttt ctatattcat gattcccctc cagaagtatt tgtgtaccag
     1501 ctttactatg atatgctgac tcaattttca aggacaattt aagcgcagaa aggaaaatgt
     1561 agcaatacag tatttgcgaa tatactcaaa aataaagttt gttgtttcag acacatgtaa
     1621 ttgctaaaga caaaatattt cgttaatcga taataccagg caatgattag tacaccagta
     1681 actatcgcca cacacaatcg cagtggagca ggttacaacg aggtgatagc ggtagtgcga
     1741 tttgctgatg gacctggcag ggaaatgcag gtaaagacgc aggcgcaggt gaacggtagt
     1801 tatggcttta cggtaagcgg agcaaatgtg tacgatactc aggggagagt cattgcgcag
     1861 gggaaaccat attttgaggc aggggaagca ctgcctatac accagtgtca atgacacatc
     1921 caaccacata tcaatacgat tgcatggggc aaacggtgaa gacaatatac actcatggtg
     1981 gcactgttat accaggttac ggcgttcgtg atcggaaatt aattgagtgc accataatga
     2041 tttattagca taactagaaa atgattagat catgctacat gattagtttc tctttcaaat
     2101 ccagaagcgt aagtacatgt ttgcgttctt cattgataag ccgttccagt atgttttttg
     2161 cttttgcatc attgcggtat atttctttca attcagaata ccatactata gaatcgcgct
     2221 cagcgtccat tgctattttg attatatcta atggctgatg aagtgaaggt gctattttat
     2281 caatatcatc tttatttgca aatactcgtg ttgtgcctat tgccttcaaa taggaatagt
     2341 attcttcggt aaacaaaccc tgagtgtctt ttaattcctt aagcatatcg gaaaatacgc
     2401 ctttatgccg taattcttca tcggctaaga actgaaaaat ctgtttaaat gattcatctt
     2461 taaatttatt tttgaacgtg gaataaaaat aaaatcctgt ttcttcaata ccaatggcta
     2521 tgtctattaa ttcttttgtt gaatacatgt aagctcctcc attgtgatag tgtaatctaa
     2581 tatacggatt tccttatttc aaggcaacac tttttgcaaa ttcatataac aatcgtacac
     2641 caaatccggt tgcattcttt ggattgtatc cacgcttctt agaataccat gatgtgccag
     2701 ctatatcaat atgagcccat ggagtgtttt caataaaatt ttttaaaaat gttgcgccaa
     2761 taattgttcc agcatttcgt acagcaggga cattaactat atcagcaata tctgatttga
     2821 tttcttcatt gtattcttta tataatggca tacgccatat tttttcaccg gtagattctg
     2881 aagcattaaa cagcgcagtg gataatgatt catcactggt taaatatgca gctactatct
     2941 ccccaaaact tacgatacat gcgccggtta atgtggcaat gtcaatgata gcttttggtt
     3001 ttaatttttt ttcagtgaaa gataacgcat cagccagtat cagacgccct tcagcatcag
     3061 tgttgcctat ttcaacagtt ttgccattat atgcagtaaa tacatccccg ggtttatatg
     3121 cggtgtgtga aatcatattt tcagcaagtg gtataaccgc atatacattg attggcaact
     3181 tcatctgtgc aatggcgttt atggtatata aacaggctgc tgcaccagcc atatcagtgc
     3241 gcatagtttc aatgtgtcct gatggttttn nnnnnnnnna aattaatgcc gcctgtgtca
     3301 aaggttatgc ctttgccaac aagagcaatt gagtcctttt tggaaggtgc acccctgtaa
     3361 tgcatcacaa taagttgtgg tggatactta ctaccctggc ttaccgccaa aagcaggccc
     3421 atcttcattt tttcaatatc ttttttgtta tatacagtgc attttatttt tgattgtgca
     3481 gcaatttttt ttgcaagttt tgcaatagtt aacggattgg cattctcggt tatttcattt
     3541 accaaatcac gacatagcaa tgtgttactg catacaatgc ttacttcttt taatagtggc
     3601 gatagttgtc caggattgtc actcaaaagg atagcttttt ccaataatgg gctggtttct
     3661 tctttttgtg ttttgtagcg attgaatgca tagtttgcaa gatatacccc ttcagcaata
     3721 taccgtgctg tgtcgttggt aacattaatt tccggaacaa caatatgtac ttccgatact
     3781 gatttatctg aacatgcacc aacgataaca cttgcagcat tgcgcaacga ttcaggtgtg
     3841 atgtctttgt ggctgccaag cccaacaaga aggattgacg gtctgtcttt gagtgctgca
     3901 aaaaaatagg tattgggttt agctttaaat gttttagtat tgataattg
//
LOCUS       DAOSZP010000150         3934 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_18587_length_3934_cov_2.861562, whole genome shotgun sequence.
ACCESSION   DAOSZP010000150 DAOSZP010000000
VERSION     DAOSZP010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3934)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3934)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3934
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18587_length_3934_cov_2.861562"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..328
                     /locus_tag="PK348_04420"
     CDS             <1..328
                     /locus_tag="PK348_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXK65490.1"
                     /translation="QAIQFMTVDMAMKLDAARLLVYRAATNAGGGLPSIYEASLAKAY
                     ANEMVVDVTNTAMQMFGGYGYSTEFPVERMYRDAKAWGVAGGTIQMLKIGIASMLYGR
                     RFDQRK"
     gene            395..832
                     /locus_tag="PK348_04425"
     CDS             395..832
                     /locus_tag="PK348_04425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65491.1"
                     /translation="MGKYYKDTEELYTIYGYFLDNILKDPKIGSKLAKAGIVIKFVYT
                     DPDAEITIDLKNPPKKEGYYGTFYLGKCDIEADVWSSQPADHSHRFWHGLENPIAGVA
                     KGIIKQGGKVTAMLKLLPVIKPTFQQFPIALKEMGREDLIVTK"
     gene            840..2720
                     /locus_tag="PK348_04430"
     CDS             840..2720
                     /locus_tag="PK348_04430"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HXK65492.1"
                     /translation="MYGNTGKIVYINCSTGKTEVVSLDEDVYKKFIGGSGLAAKLFWD
                     YADFDADPLSEKAVLIFTNGPLAGVPLSGTSRMSAVARSPLTGALAESSCGGYFPPQL
                     RYAGYDGIIISGKCTVPSIIHIAHGSFNIVSSEELWGKTVGEAYVKIKEQYGERSRTL
                     IIGPAGEHGVPYACILSDAHHAFGRCGLGAIMGSKNIKAIVIEPHQTIMKYFEEQKVK
                     SLIKELTPRIKEYIISQVLHEFGSAGNLEGHMYTGDVPIKNWTSNFNEEMAEKLTGST
                     LTEKYLTKTGTCAYCVVACKRIVKVDEGPYSIPEGPGPEYETVAALGCLLGSSDLAAV
                     CKAGRLCNEFGLDSISTGATIAWAMEAFEKGDLTEKDTGGITLRWGDMPTVIDTIIPS
                     IARKEGPLGQLLSKGSRLAAKSINNNLIKYTAQSKGLEAPMHDPRGGGHGHALAYAVS
                     PRGACHVSTAMHFMETGACNYPEIGFEFDLEALTDEKKPETMVLATAIGSIENSACFC
                     QYADRSLTFAEMVGILNAVSGYHYSLQDALDAGWRIYHLKRLINHRFGLRACDDALTE
                     RMLEPARDGEPQGIMINFDSMIQRFYELMNMDNVEGVATVDTLHKYGLEQEAAIVWKK
                     RK"
     gene            2702..2938
                     /locus_tag="PK348_04435"
     CDS             2702..2938
                     /locus_tag="PK348_04435"
                     /inference="COORDINATES: protein motif:HMM:NF014636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HXK65493.1"
                     /translation="MEKEKIGITVEGAGVLRKELFKHTVTLDCDARVEDLAKKIKIKK
                     GLQIAFFRNGKRLNRNVKLHDGDTIKVVPLVFGG"
     gene            3097..3672
                     /locus_tag="PK348_04440"
     CDS             3097..3672
                     /locus_tag="PK348_04440"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HXK65494.1"
                     /translation="MKKIIITILAIVLIGIIAVYLFLRTGLPDYSTNINALGLSAPVT
                     IERNSFAVPTITAQNMEDLFFAWGFVNAQDRLFQMEFTRRIAQGRISEFAGADALSKD
                     YFLRGVGFYERAKAYAQKLSPYYKKLYQRYVDGVNHYLDTHGTNVYMKLLGMKKEKWE
                     IADSICVGMMLNWSLANFQSTAFWLHDAHHR"
     gene            3796..>3934
                     /locus_tag="PK348_04445"
     CDS             3796..>3934
                     /locus_tag="PK348_04445"
                     /inference="COORDINATES: protein motif:HMM:NF017206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HXK65495.1"
                     /translation="MKTLVTGATGFIGSAIVKKLLSQGYCVRALVLPGEDSQWLKDVG
                     VE"
BASE COUNT         1258 a          614 c          940 g         1122 t
ORIGIN      
        1 tcaggcaata cagtttatga ctgttgatat ggcaatgaag cttgatgcag ccagattact
       61 ggtgtaccgg gcagctacca atgcaggtgg tggactgcca tcaatatacg aagcatcact
      121 tgcaaaagcg tatgccaatg aaatggttgt ggatgttacc aatactgcaa tgcagatgtt
      181 tggcggatat ggatacagta cagaattccc tgttgaaagg atgtacaggg atgcaaaggc
      241 atggggtgtg gccggcggaa caatccagat gttgaagatt ggaatagcca gtatgttata
      301 cgggcgtcgt tttgatcaga gaaaataata tctggtgatt tgtggtaaga gattggagat
      361 ttcaaattta aattaattat acttggagga cactatggga aagtattaca aggatacaga
      421 ggagttgtat acaatatatg ggtattttct ggataatatt ttaaaagacc ctaagattgg
      481 ttcaaagctt gcaaaagcag gtattgtcat taaatttgtt tatactgatc ctgatgctga
      541 gattacaata gatttaaaaa atccaccaaa aaaagagggc tattatggca ctttttattt
      601 gggtaaatgt gatattgaag ctgatgtatg gtcatcgcaa cctgccgacc actcacaccg
      661 tttctggcat gggttggaaa atccaatagc aggggttgca aaaggaatta ttaagcaggg
      721 tgggaaggtt acagcaatgc tgaaactatt gccggtaatt aaaccaacat ttcagcaatt
      781 tcctattgct cttaaggaaa tgggtagaga agatcttata gtgacaaaat gaggccgtta
      841 tgtatggaaa taccggcaaa atagtatata taaattgttc aacagggaaa actgaagtag
      901 tatcattaga cgaagatgta tacaaaaaat ttattggtgg gagtggattg gctgcaaagc
      961 ttttctggga ttatgctgat tttgatgctg atccattatc agaaaaagct gttttaattt
     1021 ttacaaatgg gcctcttgca ggtgtgccgt tgtctggaac aagcaggatg agtgcagtgg
     1081 cacgttcacc tttaacagga gcactggctg aatcttcgtg tggcggatat ttccctccac
     1141 aattgagata tgcagggtat gatggtatca ttatatcagg aaaatgcaca gtgccatcta
     1201 tcatccatat tgcacacggt tcttttaata ttgtatcttc agaagagttg tggggcaaga
     1261 cggtaggaga agcttatgta aaaattaagg aacaatatgg tgaaagaagc agaacgttaa
     1321 taattggtcc tgcgggtgaa catggagttc cgtatgcatg catactcagt gatgcacacc
     1381 atgcatttgg caggtgtgga ttgggcgcca ttatgggttc taaaaatata aaagctattg
     1441 tgattgaacc acatcaaact ataatgaaat attttgaaga acaaaaagta aaaagtctta
     1501 ttaaagaact tactccacgt atcaaggaat atatcatttc acaggtttta catgagtttg
     1561 gttctgccgg aaatttagag ggccacatgt atacagggga tgttcctatt aaaaactgga
     1621 catcaaattt taatgaagaa atggcagaaa aattaactgg gagcacctta accgaaaaat
     1681 atttaacaaa gactggaaca tgtgcatatt gtgtcgtagc atgtaaaagg atagtaaaag
     1741 ttgatgaagg gccatatagc atacctgaag gtccaggacc ggaatatgaa actgtggcag
     1801 cattaggatg tttacttggt tcatcggatc ttgctgcggt atgtaaggcg ggaagattat
     1861 gtaatgaatt tggcctggat tccatatcaa caggagcaac aatagcatgg gcaatggagg
     1921 catttgaaaa aggagattta actgaaaaag atactggtgg tataacactg cgatggggtg
     1981 atatgccaac cgtaatagat accattattc catcaattgc cagaaaggaa ggacctttag
     2041 gacaattact atcaaagggt agcagattag ctgctaaaag tattaataat aatttgatta
     2101 aatacaccgc acagtcaaaa ggtttggaag caccaatgca tgatccacgt ggtggaggtc
     2161 atgggcatgc tttagcatat gcagtcagcc cgcgaggtgc ctgtcatgta tccacagcaa
     2221 tgcattttat ggaaacggga gcatgtaatt atcctgaaat tggttttgaa tttgatttag
     2281 aagcattaac agatgagaaa aagccagaga caatggtact ggcaacagct ataggttcta
     2341 tagaaaacag tgcgtgcttt tgtcagtatg cagaccgttc gctaacattt gctgaaatgg
     2401 ttggtatact gaatgctgtt tcagggtatc attattcatt acaggatgcg cttgatgcag
     2461 gatggcgtat atatcattta aaacggctca tcaatcacag atttggtttg agggcttgtg
     2521 atgatgcgtt gacagaacga atgctggaac ctgcacgtga tggtgaacca cagggaatta
     2581 tgattaattt tgatagcatg attcagcgtt tttatgaact tatgaatatg gataatgttg
     2641 aaggagtagc aacagttgat acgttacaca aatatgggct tgaacaggaa gccgcaatag
     2701 tatggaaaaa gagaaaatag gaattactgt tgaaggtgct ggtgttttgc gtaaagaact
     2761 gtttaagcat acagtaactt tagattgcga tgcacgggtt gaagatttag caaaaaagat
     2821 aaaaataaaa aaaggccttc agatagcttt ttttagaaat gggaagcgct tgaacaggaa
     2881 tgtaaagctt catgatggag ataccataaa agttgttcct cttgtttttg gaggttagat
     2941 aataattctt acaaaatggt ggcgcacgtc tcctatgtat tttaaacaaa gtatttgaca
     3001 atcctattat tccagcatat taataatata ttatatcttt ttgtactgct catctatcag
     3061 tgaattaaat agagaacgtt attacggggg tttgccatga aaaagattat tataacgata
     3121 ctggccattg tacttatagg tattattgca gtttacctgt tcttacgaac tggattgcct
     3181 gattactcca ccaatattaa tgcactaggg ctttcggcac cagtaacaat agaaagaaac
     3241 agctttgctg tgccaactat caccgctcag aatatggaag accttttctt tgcatggggc
     3301 tttgtcaatg cacaggacag gttgtttcag atggagttta cgcggcgaat tgctcaggga
     3361 cgaataagtg agtttgcagg agcggatgca ctgagcaagg attacttttt gcgaggggtt
     3421 ggcttttatg aaagggcaaa agcttatgca cagaaactat cgccctatta taaaaaatta
     3481 taccagcgtt acgtggatgg tgttaaccat taccttgata ctcatggcac caatgtgtac
     3541 atgaaacttt taggcatgaa aaaagaaaag tgggagatag ctgattccat ttgtgttgga
     3601 atgatgctta actggagcct ggccaacttt caatccacag ccttctggct acatgatgct
     3661 catcaccggt gaaaatgaac actttatgag taagcacaat acagatatgg tagatgcctg
     3721 gcgagagcac aagtattata ctgtcggcaa ggaggagata gtgtattcaa tgaaactggt
     3781 accactgaaa tagcaatgaa aactttagta accggcgcaa ctggttttat tggttcagct
     3841 attgtaaaaa aactgctttc acagggctat tgtgttcgtg cactggtgct gcctggggag
     3901 gatagccagt ggctaaagga cgttggtgtt gaag
//
LOCUS       DAOSZP010000151         3915 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_18707_length_3915_cov_3.158290, whole genome shotgun sequence.
ACCESSION   DAOSZP010000151 DAOSZP010000000
VERSION     DAOSZP010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3915)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3915)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3915
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18707_length_3915_cov_3.158290"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(103..1314)
                     /locus_tag="PK348_04450"
     CDS             complement(103..1314)
                     /locus_tag="PK348_04450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65496.1"
                     /translation="MKFVKSMVLVAVLAVATAGFAQEAKPEEKVPDKPKLAVDGLAYL
                     EWSKELNHSKLGDPDENTNTFAIKRVYLNFKKKLDDIWSVRVTTDVGQVEAKSDKDYV
                     TQSGTPDTKSKVESKTNYYTVFLKYAYVEAKQKFDIADVKFQFGMIGTPLIGFVDKQS
                     DYRWLEQNYVDQAKTLLYYAATPADVANPGKWKTQSIDHSADMGLSLEVSMFNKLLTL
                     TGAYTNGEGYKYADEVKLGDDGKAYYGMATIMPVKDLYIFGIYRNQDTNDKIGDNYIR
                     QMGGGVVYSDGLFKIGAMYLLPEVSTKATGASAVENKYKLFDSWLNIRLDTIISMPVI
                     VVGRYAFGENKDLDKSKVTSWAAGLGYHVANGLRAVVYYQDTKADFKEKSAGFTGDSD
                     RKIFVKTEVKF"
     gene            1981..2772
                     /locus_tag="PK348_04455"
                     /pseudo
     CDS             1981..2772
                     /locus_tag="PK348_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418801.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
     gene            3015..>3915
                     /locus_tag="PK348_04460"
     CDS             3015..>3915
                     /locus_tag="PK348_04460"
                     /EC_number="1.8.4.11"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010882079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methionine sulfoxide reductase B/A
                     protein"
                     /protein_id="HXK65497.1"
                     /translation="MKNENLTPQQYYVFRQCGTEPPFNNEYWDNKKHGIYVDIVTGAV
                     LFSSTNKFDSGTGWPSFTQPVQPDAVVERSDTSHGMTRTEVRSKSSDGHLGHVFDDGP
                     APTGLRYCINSASLRFIPVEKMIEEGYADYMYLFEGIQLPFEQIILGGGCFWGVEAYF
                     KKIAGVIDTVVGYSGGTTLNPDYKTVCSGTTGHAETVLVTFDPKIITLEKILKHFYAI
                     HNPSTPNRQGPDIGTQYRSAIFYVSDRQKHTIDEMVGKLKANGITVTTEVAKAKNFYK
                     AEEYHQDYLTKNPGGYCHINLNKI"
BASE COUNT         1240 a          779 c          699 g         1197 t
ORIGIN      
        1 aagctcaaag gtattgcact atggctatta ggatttgaat aaaaaaatga gggaggcttg
       61 catgcttccc ccattcttgg gatagaatta tggttgatgt tgttagaatt taacttctgt
      121 ttttacaaaa atctttctgt cggaatcacc agtaaaacct gcagactttt ctttaaaatc
      181 agcttttgta tcctggtaat ataccacagc tcgcagacca ttggcaacat gatatcctaa
      241 ccctgctgcc catgatgtta ccttagattt gtcaaggtcc ttgttttcgc caaaagcgta
      301 tctaccaact actataacag gcatgctgat gatagtgtct aaccttatat tgagccagct
      361 atcaaacaat ttgtacttgt tttcaacagc ggaagcccct gtagcttttg tactcacttc
      421 aggaagcagg tacatagcac ctattttaaa aagcccatca gagtaaacca caccaccacc
      481 catctgtctg atgtagttat caccaatttt gtcattggta tcctggttgc gatagatgcc
      541 aaaaatatac agatctttaa ctggcataat cgttgccatg ccatagtatg ccttgccatc
      601 atcacccagt tttacctcat cagcatattt atacccttca ccgttagtgt atgcaccagt
      661 gagtgttaaa agcttattga acatggaaac ctctaaactc aaacccatgt cagcagaatg
      721 gtcaattgac tgtgttttcc atttgcctgg attggctaca tctgcaggag ttgcagcata
      781 gtaaagtagt gtctttgcct ggtcaacata gttttgttcc agccatcggt aatcactctg
      841 cttgtcaaca aaaccaatga gtggtgtacc aatcatacca aactgaaact taacgtcagc
      901 aatgtcaaac ttttgttttg cttcaacata agcgtacttt aaaaatacag tgtaatagtt
      961 agtcttggat tcaacttttg attttgtatc aggagttcct gattgagtca cataatcctt
     1021 atcactttta gcctcaacct gaccaacatc tgtggttacc cgcacactcc atatgtcatc
     1081 aagcttcttt ttgaaattta aataaacccg cttaatggca aatgtgttgg tattttcatc
     1141 gggatcacca agctttgaat gattcaattc ctttgaccac tctaaatatg ccagtccatc
     1201 aactgccaat tttggtttat ccggtacctt ttcctctggt tttgcttcct gtgcaaaccc
     1261 agcagttgca actgcaagca ctgctactaa caccatactc tttacaaact tcattaaaac
     1321 ctccttaaac gttagttaaa ttattgatgt tatgattttt caaaatctga ttgtaaataa
     1381 gcagcactat ataaagtaat tttgtggcaa ttgttaagat tttataaatt ttcttaattt
     1441 tgtcttctat tatcttttac tcatctgctc ttacatatca tgcatggtac atatttcatg
     1501 tgtgtttatt tgatcatact aagtacgctg aacaaagtat ggtacaaata tttttgtttc
     1561 ttataatcca cgtatcctac ggatatactg tggtatatct tttatataaa aaaattggat
     1621 tcagtctaaa atatttttac aatacatttc cacttagctg cataacaacg taatcctgta
     1681 ttgtgtattg ggattaataa ttatttccaa ataaagagta ttgacaaaat gatatatttt
     1741 tttacatttt ccaatcgtga ctcaatactc tcatttttaa caattaacta tctctcaata
     1801 aaggtggtaa acgggaaggc ttatgatgtt taatccatta acaacacgat tatgtcagtg
     1861 atgtgcatat aaggggccag gttggttccc ttttatccgg tttaccattg cctgcctgcc
     1921 ataacttcat aaagcttcct taaagttccg gcacaggtgg tggccggtat atttaacttt
     1981 attgatcttc ctcagatgaa atctgatgaa ggcaaaaaac tcctgaaaca ggagtataaa
     2041 aagtttatga caaaacttga gcaaataagc ggtaaaaaaa ttacagcaga ttcacttaaa
     2101 aagggaattg aaattaccaa cgccaagcgt aatgctgtta aacgtttagc aacacttcga
     2161 aaagccaatc ccgttcccat atcaggactt gatgcattgc tggtgaatca ggtgtttttt
     2221 tatgatgatc ctgtacgatt tactgaatca gtcaacaagc tatgtgatga actagaagag
     2281 cgaataaaaa ataaggtggg tgtattccct gaacacacaa aacgtcttgt catttctggt
     2341 tgtcccatgg ctgttccaaa ctggaaggta ccatggatta ttgaaacaag cggtgcaact
     2401 atagttggcg aagaatcctg tgttggcgag cgtggtacac agtggattac tgaagccaga
     2461 ggcaatactg ttgacgaatt acttgatgca attgttgagc gttacttcca gatagattgt
     2521 gcaatcttta caccaaatcc ttcacggctt gaacatatca aatccatggt aaaagagctt
     2581 aaagcagatg gtattatcca ttatggatta cagttctatc agccatatat tattgaaagc
     2641 cataccgtgg aaaatagcct tgaaaaggat ggcattccaa cactgcgtat tgaaactgat
     2701 tatggtcagg aagatgccgg acagcttaaa acccgtattg aagcatttgt tgagatgctg
     2761 ggtaataaat aatataacaa tacgcataga ctcaacatta aaatcaaaac gcgaaaaaac
     2821 tgctaaaaga agtaatattt caaaatacaa acctttgacc ctttaccgat aactatcgcc
     2881 ccatattatt tgtaataatt acaatatgct attgactttt gaataaaaat tattatattt
     2941 ataccaccat gaaacagatt ttcatattta ttatacttat agtaagctgt aactattatt
     3001 acaaggaaca acccatgaaa aatgaaaatt taacaccaca acaatattat gtattcaggc
     3061 aatgcggcac cgagcccccg tttaacaatg aatactggga caacaaaaag catggcattt
     3121 acgttgacat tgttacgggc gcggtgcttt tttcctcaac caacaaattt gactctggca
     3181 cgggatggcc aagctttaca cagccagtac agcctgatgc agtagtggaa cgctctgaca
     3241 cctcacacgg tatgacacgc accgaagtgc gcagtaaaag ctctgatgga caccttggtc
     3301 atgtatttga cgatggtccc gcaccaacgg ggctgcgata ctgcattaat tcagcatcat
     3361 tacgctttat tccggttgaa aagatgattg aggaaggtta tgcagattac atgtatcttt
     3421 ttgaaggtat acaattgcca tttgaacaga tcatccttgg tggtggttgt ttctggggcg
     3481 ttgaggcata cttcaaaaaa attgcagggg taattgacac tgtcgttggc tacagcggtg
     3541 gaaccacact aaaccctgat tataaaactg tgtgcagcgg tacaacagga cacgctgaaa
     3601 cagtactggt aacatttgac cctaaaatca ttacgttaga aaaaatttta aagcatttct
     3661 atgccattca caatccctca acaccaaacc gtcagggacc ggatattggt acacagtacc
     3721 gttcggcaat attttatgta agcgataggc agaagcatac aattgatgaa atggtaggaa
     3781 aactaaaagc aaacggcatc acggtgacca ctgaagttgc aaaagccaaa aatttctata
     3841 aagctgaaga ataccatcag gactatctta caaaaaatcc aggtggatac tgtcacataa
     3901 atttaaataa aatac
//
LOCUS       DAOSZP010000152         3908 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_18751_length_3908_cov_3.053465, whole genome shotgun sequence.
ACCESSION   DAOSZP010000152 DAOSZP010000000
VERSION     DAOSZP010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3908
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18751_length_3908_cov_3.053465"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3444)
                     /locus_tag="PK348_04465"
     CDS             complement(<1..3444)
                     /locus_tag="PK348_04465"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HXK65498.1"
                     /translation="MTPTIQKGFIAVMIVLVICFGITTAYVLNTNEIKLTIDYDNNTP
                     YTELSLQFSRDIFIVSDIDAMKNDIAQKLTVSFNPELEYILKPLDSRNCILEIYNPKP
                     AHEYQFTIIVKNIDTNGLYVTANNKEIELGKTYFFRTPVLRVYHCSKESKMINSPIVL
                     QFNFPVSLNVLMQKMSIRPDVQYTLEYDSNDTSHKTVIVKPINEKKAQKYSLTIDKTM
                     VPASGVIGMDTSYTFTYTTYFPFELTDIQSSYSRYYDDSSYYPDYPVEFTFNNEIALP
                     QGKSVSEFVTVTPDPGGLQVNMYGNTIVVSGNFTGKKTYTVTIAQTLQDIYGQTLQEP
                     VETEIEFEHAFSYFSCPTGYMLLENYLEKIIPLRMVNVSSFECRYLSLVNENDIKAYF
                     ESPKEYFNKHAKTKTYILNWTWDVYKTVKFDYSKLHQGDNGIFVYELKPVMEHPYENN
                     HDVYRGMMQLSDIGVTIKKTKNDILVHVRDLLNNKALAGASLFCTGAEVSLGQTDAKG
                     VLIVKNNGCSLFTIKHSKSIFHYSMQRDSGYSDEDERAKINLPEKYYGSDYYILKPQV
                     HLFTDRYLYRPGETVHVKGILRYRNNDQWSIKPPLSVIKNVMLTVINSRDEEIASSAR
                     SVSATGGIEWDIALKEDYPTGYYRITVSSSNKDYDLYQSIDFQVEEFKPAKAEMKIIP
                     SKLTFLWGEILSVDLFGWYLFGAPVNNDVEYTVTLQPVTYRSSVFADYSFSTQLNYYD
                     EYDEYSPPSTVVASGKVKVNEKGIAKVHVPLKAIANVPNAEILITGRTYLSDDSPVFG
                     AKSGLTVAEPVHVGIKVPRYFNYTKEPVTIQLVACNNDDTVVEGTPIKLVIKKHEWKS
                     FQIAGVNGRLQWEYKKIETEVNLQNHTIGKKDVVATINDPGYYTAEAYDSQGKRKYAV
                     CDFYVLGKGEAGWRVNDDETVDVETDKKSYNVGETAMLLVKNPFTKAQAFVSIEREKF
                     YDYFEVPATSSVVMIPVKITKEHIPNIYVSVMLYSGRTGFNALKDGEDRAKPRCLIGY
                     ARIKVTPKEKKLSVQVKPDKKQYEPGGYTTVELLVKDYKGQPVDGEATISIADRGVLN
                     LVNYTLPDPYEYFYAPRQLAVTTFDVHQIILGQRYLKEKGELIGGDGGIASMGMIVPR
                     AD"
BASE COUNT         1194 a          767 c          604 g         1343 t
ORIGIN      
        1 atcagcccgt gggacaatca tacccattga agcaatgcca ccatcaccac ctatcagttc
       61 gcccttttct ttaagatacc gctgcccaag aataatctga tgtacatcaa aggtagtaac
      121 tgcaagttgt cgcggggcat aaaagtattc atagggatca ggtaatgtat aattgacaag
      181 atttaataca cctctatcag caatagatat tgttgcctct ccatccaccg gttgtccttt
      241 ataatctttt accagcaatt ccaccgttgt atagccaccc ggctcatatt gctttttatc
      301 tggtttaacc tgaacagata atttcttttc ttttggtgta actttaattc tggcgtatcc
      361 aattaaacag cgtggttttg ccctatcctc cccatctttc aaggcattaa accctgttct
      421 tccagaatac agcataacac ttacataaat atttggaata tgctcttttg ttattttgac
      481 cggaatcata acaacacttg atgttgcagg cacttcaaag taatcataaa atttttctct
      541 ttcaatacta acaaatgcct gagctttggt aaaaggattc ttaaccagaa gcattgctgt
      601 ttcaccaaca ttgtagcttt ttttatcagt ttcaacatca acagtttcat catcgttcac
      661 acgccaccct gcttcgcctt tgcccagaac gtaaaaatca cacactgcat attttctttt
      721 tccctggctg tcatatgctt ctgctgtata gtacccagga tcattaatag ttgctactac
      781 atcttttttc ccaatagtat gattctgtag attgacttcg gtttctattt ttttgtattc
      841 ccattgcaat cttccattta ctcccgctat ctgaaatgat ttccattcat gtttttttat
      901 aactaatttt attggagtac cttcaacaac agtatcatca ttgttgcagg caacaagctg
      961 tatggtaaca ggctcttttg tataattaaa atagcgcgga acctttatgc caacatgaac
     1021 cggttctgcc accgtaagtc cactctttgc accaaatact ggcgaatcat ctgaaagata
     1081 cgtccgtcca gtaatcaata tttctgcatt gggcacattg gcaatagctt taagcggaac
     1141 atgcaccttt gcaatgcctt tttcatttac ttttactttg ccactggcaa caaccgttga
     1201 tggtggacta tactcatcat attcatcata ataattaagc tgtgttgaaa atgaataatc
     1261 agcaaatacg ctactgcgat aggttaccgg ctgcaatgta accgtatatt ccacatcgtt
     1321 atttaccggg gcaccaaata aataccatcc aaacaggtca accgaaagaa tttcccccca
     1381 caaaaacgta agctttgatg gaataatttt catttcagct tttgccggtt taaactcctc
     1441 aacctggaaa tcaatacttt gataaagatc ataatcttta ttactagaac taacagttat
     1501 gcgatagtac ccggtaggat agtcttcttt aagtgctata tcccactcaa tgccaccggt
     1561 tgctgacact gacctggcag aagaagcaat ctcctcatcg cgcgaattaa tgacggtgag
     1621 cataacattt tttattactg acagcggtgg tttaatactc cactggtcat tattcctgta
     1681 cctgagtatt cctttgacat gaactgtttc acctggcctg tataaatatc tgtcggtaaa
     1741 aagatgcacc tgaggcttta gtatgtaata atcactgcca taatattttt ctggaagatt
     1801 aattttggca cgttcatctt catcgctata tccactatcg cgctgcattg agtaatgaaa
     1861 aatgctttta ctatgtttta tcgtaaacag cgaacatcca ttatttttaa caatcaaaac
     1921 acctttagca tctgtttgtc ccagcgacac ttccgcccca gtacagaata gtgacgcacc
     1981 ggctaatgcc ttgttgttca ataaatctct gacatgtact aatatatcat tttttgtttt
     2041 tttaatggtc acaccaatat ctgaaagttg catcatgcca cgatagacat catgattatt
     2101 ttcatacgga tgttccataa ctggctttag ctcataaaca aaaatcccat tatctccctg
     2161 atgcagtttt gaataatcaa acttaacagt tttatacaca tcccatgtcc agtttagtat
     2221 atacgttttc gtttttgcat gtttattaaa atattctttt gggctttcaa agtatgcttt
     2281 aatatcgttt tcatttacaa gcgatagata cctgcattca aatgatgaaa cattgaccat
     2341 gcgtaaagga ataatcttct caagatagtt ctccagcagc atatatcctg ttggacatga
     2401 aaaatacgaa aatgcatgct caaattcaat ttccgtttca acaggttcct gtaatgtttg
     2461 tccatagata tcctgtaatg tttgtgcgat agttactgtg tatgtttttt tgccagtaaa
     2521 atttccactc accacaattg tgttaccata catattcacc tgcaaaccac ctggatcagg
     2581 tgtcacagtt acaaattcgc taacactttt cccttgagga agagcaattt cattgttgaa
     2641 agtaaattca acaggataat caggataata tgatgaatca tcatagtaac gactgtatga
     2701 tgattgtata tcggttagct caaacgggaa ataggttgta taggtaaatg tataggaagt
     2761 atccatgcca atgacaccac tggctggcac catggtttta tcaatggtca atgaatattt
     2821 ttgtgctttc ttttcattga tgggcttcac tattactgtt ttatggctgg tatcattgct
     2881 atcgtattcc agtgtatact gaacatccgg acgtattgac attttttgca tgagaacatt
     2941 gagtgaaaca gggaaattaa attgcagcac aattgggctg tttatcattt tgctttcttt
     3001 ggaacaatga taaacccgca gcacaggagt tctgaaaaag taggttttcc ctaactcaat
     3061 ttctttatta tttgctgtta catataaccc attggtatca atatttttta caataatggt
     3121 aaactggtat tcatgtgctg gtttgggatt atatatttca agaatgcaat tacgtgaatc
     3181 caaaggtttt aatatatatt caagctcagg attaaatgat acagtgagct tttgagctat
     3241 atcatttttc attgcatcaa tatctgaaac tataaatata tctctggaaa attgcaatga
     3301 tagttcggta tatggtgtgt tattatcgta atctatcgta agcttaatct cattggtatt
     3361 taaaacatat gcagttgtta taccaaaaca aatcaccaga acaatcataa cagctataaa
     3421 gcctttttgt attgtaggcg tcatggctac ctcctctgaa aacaatagtt aatcattgta
     3481 tcacttcaat gccatttcta caatgttctc aatgattaca acgcttctat acataatgac
     3541 agctttttat gattatataa tggtaacgta atcagtaaat gaataacaac acatacatca
     3601 tgtaatatat ataaataaga aagtaaagtt ataaattacc cctatcatat agtataaata
     3661 ttgaacttca tcactcaata cctctcatta ttttgttctg atattacatt atttataata
     3721 tgtatatata ttattaacta tctaaaaaaa ttattaatta taataaatat ttttattgac
     3781 attataagat tgtattatta taatattttt aatgagatta tttttctatc atggaggaaa
     3841 cttatgaatt actggaatga atacaaagct aaattaacaa cacccgatga tgcagtcaaa
     3901 gttgttga
//
LOCUS       DAOSZP010000153         3883 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_18904_length_3883_cov_3.573145, whole genome shotgun sequence.
ACCESSION   DAOSZP010000153 DAOSZP010000000
VERSION     DAOSZP010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3883
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18904_length_3883_cov_3.573145"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..762)
                     /locus_tag="PK348_04470"
     CDS             complement(<1..762)
                     /locus_tag="PK348_04470"
                     /inference="COORDINATES: protein motif:HMM:NF012981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HXK65499.1"
                     /translation="MTKKDTVLLIVNPIAGRGKALRSVERIENDFKTLNIKHDVVLTE
                     RVWHAAELAEDAAKKGYRAVVVASGDGTANEVLNGLMLARKKGYNKTAMGQIPVGTGN
                     DFAYGMGLPNDIDECIKIIADFNVRRIDVGFIKGGDYPEGRYFGNGIGIGFDATTGFV
                     ASRIRFLSGMMVYLLAALQTIFIYYKAPRIRMIYNSKETIMDALQVSIMNGKRMGGGF
                     HFAPDGSPFDGLLDLCMVRSMNQFKIFGMIPYFIRG"
     gene            complement(845..2311)
                     /locus_tag="PK348_04475"
     CDS             complement(845..2311)
                     /locus_tag="PK348_04475"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXK65500.1"
                     /translation="MDNPVSLEYTTLLNTIQAPVFVVDASKNIIDINNYAKRLFHSLD
                     VIGKSCKDIFPECQKSCTCPVDEADYKIPTDSQRCKQVMVLNTVKEEVELCIIPVQTA
                     HDIVFLHILTDNDIQNRQNLVMLEKNMTISTLASGIAHEFNNMNAGIQGIVELLLSQE
                     ELSSAGVKDLKTILKIVKRATHLIDQLSMLAHRKPSKQMLVNIEDIVDDCISIIKPQF
                     MDAGITIDVIHKDRLPELFLDANKITLALMNIFLNARDAMIDTEQKKLAIITGQDDTY
                     VTITIKDSGCGIKEEHIQRIFEPFFTTKGPLGHSNIPGTGLGLSVALGIIEEHGGTLA
                     VESTVGEGTTFTIQLPVNTDDKVASEVAINYTEFDFSGKKILIVEDDPEFATILQRAL
                     IAKNAEVVYAGNGAEGLYYLDNQTVDLALLDVHLPDMTVWDILKKVQYMNNRPNIIIV
                     SGNYLAMNNNYIGIVDYVLLKPFDLEELFTAIKSSLTI"
     gene            2559..3467
                     /locus_tag="PK348_04480"
     CDS             2559..3467
                     /locus_tag="PK348_04480"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HXK65501.1"
                     /translation="MNNKPIIGIVGAGAMGSLFAFFLGKYTILLYDTNPNTVNAIKQG
                     LSVEIHSEKQTFYPTISNDPAILASADIIFLFIKSFATRDAIDTIQHYIKPDAIVVTL
                     QNGIGNVELISSMINNPLVYGTTTIGATKLSPASVRFGGTGSIIIGGDSYSHCSTVHS
                     LLQNANLTVTIAPDPKKALWEKAIINAAINPLGALLEVPNGKLIENNNTVKIMAGIIQ
                     EACLVAQANGIMTDSNHMIETTTTVCRNTQNNFCSMLQDILAKRKTEIDSINGAIITF
                     AQQYSIDTPYNTTVYKLIKYKESQLI"
     gene            3547..>3883
                     /locus_tag="PK348_04485"
     CDS             3547..>3883
                     /locus_tag="PK348_04485"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HXK65502.1"
                     /translation="MGDSTQKKCHYTVENGIALLTIDNPPMNALNHVVLKDMRETIEQ
                     LIENDSVRVVIVTGAGKAFVAGADINEIKEQNTREDGANFLKNGQDVLNIIENADKPF
                     IAAINGYCLG"
BASE COUNT         1296 a          756 c          598 g         1233 t
ORIGIN      
        1 accacgtata aaataaggta tcatgccaaa aatcttaaat tgattcatag aacggaccat
       61 gcataaatca agcaaaccat caaaagggct accatctgga gcaaaatgaa aaccaccacc
      121 catacgttta ccattcatta tggaaacctg caaggcatcc ataatggttt ctttgctatt
      181 atagatcatg cgtattcgtg gtgctttata atatataaat attgtctgca aagcagcaag
      241 cagatatacc atcataccgc ttaaaaagcg tattctgctt gcaacaaacc cagtagtagc
      301 atcaaaacct atcccaatgc catttccaaa atatctgcct tcaggataat ctccaccctt
      361 aataaaacca acatcaatcc ttcggacatt gaagtctgct attattttta tacactcatc
      421 tatatcatta ggtagtccca tgccataggc aaaatcattt cccgtaccta ccgggatttg
      481 acccatggca gttttattat agcccttttt ccgagctaac atcaatccat ttaacacttc
      541 attggcagta ccatcaccgc tggccaccac aacagcacgg tatccttttt tagcagcatc
      601 ttctgctaat tccgctgcat gccatactct ttccgttaaa acaacatcat gtttgatgtt
      661 caaagtttta aaatcgttct caatcctttc tacggatctg agtgcctttc ccctgccagc
      721 aatgggattt actataagca aaacagtatc tttttttgtc ataaatgctc ctgatatctc
      781 tgccaaaatt tgtgaagata ttatacagat ttatattttt gtcaatacta tttttatatg
      841 tatactatat agttaaactg cttttaattg cagtaaacag ttcttctaaa tcaaaaggtt
      901 tgagcagcac ataatcaact ataccaatat agttgttatt cattgccaaa taatttcctg
      961 atacaataat tatatttggt ctgttattca tatactgaac tttcttcaat atatcccata
     1021 ctgtcatatc aggtaggtga acatcaagta atgcaagatc tacagtttga ttatccagat
     1081 aatataatcc ttcagcaccg tttcctgcgt atactacttc agcattttta gcaatcagcg
     1141 ccctttgcaa aatagtagca aattcaggat catcttcaac aatcaaaatt tttttacccg
     1201 aaaaatcaaa ttcagtataa tttatggcaa cttcagaagc aactttatca tcagtattaa
     1261 cgggcaattg tatggtgaat gtagtacctt cgccaactgt gctttctact gctaaggttc
     1321 caccatgttc ttcaataata cccaatgcaa ccgaaagccc cagtcctgta cctggtatat
     1381 tggaatgccc caatgggcct tttgtggtaa aaaatggctc aaatatcctc tgtatatgtt
     1441 cttcttttat tccacaacct gaatctttaa tagttatagt aacatatgta tcatcctgac
     1501 cggtaataat tgctaatttt ttttgttctg tgtcaatcat agcatcacga gcatttaaaa
     1561 aaatattcat aagagccagg gtgattttat tggcatctaa aaataattct ggtagtctgt
     1621 ctttatgaat aacatcaata gtaattccag catccataaa ctgtggtttt ataatactta
     1681 tacaatcatc gactatatct tcaatattaa ccagcatctg ttttgaaggt tttcggtgag
     1741 ctaacattga taactgatca ataagatgtg tggcacgttt aactatcttc aatattgttt
     1801 taagatcttt tacaccagcc gaagataact cttcctggga aagcaataat tcaacaattc
     1861 cctgaatgcc tgcgttcata ttattaaatt catgggcaat acccgatgcc agcgttgaaa
     1921 tagtcatgtt tttttcaagc attaccaggt tctgcctgtt ctgtatatca ttgtcagtta
     1981 aaatatgaag aaatacaata tcatgtgcag tttgtaccgg aataatacac aattccacct
     2041 cctcttttac cgtattcagt accattacct gcttacaacg ctggctatca gttggaattt
     2101 tgtaatcagc ttcatcaaca ggacaggtac atgatttttg gcactccggg aagatatcct
     2161 tacacgattt cccaattaca tcaagagaat gaaataaacg cttagcataa ttatttatat
     2221 caataatgtt ctttgaagca tctaccacaa aaactggtgc ctgtatggta ttaaggagcg
     2281 tagtatattc caaactgaca ggattatcca tggtaataat ttctcgttag caattttttt
     2341 acaatcctaa tgataactaa tcaaatttta atagttatat tatatcataa aaatagtaca
     2401 tttttatcac attatcaaaa ttttaataca aatatactat atggttgcag gagtcaaaat
     2461 atttttaaga aaatattcat gaatttatat ataatactat ttagcaaata tattaacagt
     2521 attgacacca taaagtaact atgatgtaaa aaaaaatcat gaataataaa cccattatcg
     2581 gaatagttgg cgcaggtgca atgggatccc tgtttgcatt tttcctgggc aagtatacca
     2641 tactattgta tgatactaat cccaatacag ttaatgcaat aaagcagggt ttgtctgttg
     2701 aaatacattc agaaaaacaa accttttatc ccactataag caatgatcct gcaattcttg
     2761 cttcagcaga tatcatcttt ttgttcatta aaagttttgc caccagagat gcaattgata
     2821 ctattcaaca ttatattaag cccgatgcaa tcgtagtaac attgcaaaac ggtattggca
     2881 atgtcgagtt gatttcgtct atgattaaca acccgttagt ttacgggacc accacaattg
     2941 gcgccacaaa actttcacca gccagtgtac gttttggagg aacaggctct atcatcattg
     3001 gtggcgatag ttatagccat tgttccaccg tgcattcatt actacaaaat gccaacctga
     3061 cagtaactat cgcccctgac cccaaaaaag ccttatggga aaaagctatt atcaatgcag
     3121 cgattaaccc actgggagca ttgctggaag tgcctaatgg caaacttata gaaaacaata
     3181 atacagtaaa gattatggca ggtatcatac aggaagcatg cctggtggca caggctaacg
     3241 gtattatgac agatagtaac catatgattg aaacaactac aacggtatgc agaaacacgc
     3301 agaataattt ttgttcaatg ctacaggata tacttgctaa gcgaaaaact gaaattgatt
     3361 ctattaatgg tgctattatt acatttgctc aacaatatag catagatact ccctataata
     3421 caacagtata caaacttatt aaatataaag agtcacaatt aatttaaatt ttatatttac
     3481 aattaaatct tgacaaacct gcaccatatt tgaaaatttt agaaaattta attcaagagg
     3541 tgtgatatgg gtgactctac acaaaaaaaa tgtcattata ctgttgaaaa tggaatagct
     3601 ctattaacta ttgataaccc accaatgaat gccttgaacc atgttgtttt aaaagatatg
     3661 agggaaacaa ttgaacagct cattgaaaac gattcagtta gagttgttat tgttaccggt
     3721 gcaggcaaag cgtttgttgc tggtgcggac attaatgaaa ttaaagaaca gaataccaga
     3781 gaagatggcg caaactttct taaaaacggc caggatgtgc tgaacatcat tgaaaatgct
     3841 gacaaaccat ttattgcagc tattaatggc tactgcctgg gtg
//
LOCUS       DAOSZP010000154         3874 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_18946_length_3874_cov_2.648337, whole genome shotgun sequence.
ACCESSION   DAOSZP010000154 DAOSZP010000000
VERSION     DAOSZP010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3874
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18946_length_3874_cov_2.648337"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /locus_tag="PK348_04490"
     CDS             <1..467
                     /locus_tag="PK348_04490"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HXK65503.1"
                     /translation="KKVAKNVGILIELAKQYSLPIILTEQYRKGLGPTIKELMDRLEG
                     VVAFDKLAFDCMKDNTIREQVTRIKRTNIILCGIETHICVLTTALSLLKENYNVIIAS
                     DAVCSRRTHEWEMAIHALRQAGAIVYPTETISFMILEKAGTERFKQLSPLFK"
     gene            complement(469..>946)
                     /locus_tag="PK348_04495"
     CDS             complement(469..>946)
                     /locus_tag="PK348_04495"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HXK65504.1"
                     /translation="FSVVTGKKALLVGGGVGNAPLYYLAKMLRRVNTQVTYIYGSRSA
                     SHIFLLDRYQELSHTCIVTTDDGSLGIKGNPIDVAKDIVAKHKFDIVYTCGPEIMMKR
                     LSRELSHIPIEASLERYFGCGVGLCSGCAVATINGYRRACIDGPVFDAHTVLWDTP"
     assembly_gap    947..956
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<957..1219)
                     /locus_tag="PK348_04500"
     CDS             complement(<957..1219)
                     /locus_tag="PK348_04500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HXK65505.1"
                     /translation="MITLIETKPVADDHYLLTLHCDAKESIPGQFINIKVNTSTDPLI
                     RRPFSIFDHDQTTIHIVIRVVGKGTERIVALHPGPVDCIGPFG"
     gene            1339..1680
                     /locus_tag="PK348_04505"
     CDS             1339..1680
                     /locus_tag="PK348_04505"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HXK65506.1"
                     /translation="MIVAMSKLTPYEEKLINDFVEYITSVPYVNSIVVYGSRSKGVSN
                     EYSDVDIAVVIDNLHNSKKVEKLIEQWNIDNSPEILVHFLVIDTNNLHSSAIGKEIEK
                     GDTVWLRQRKG"
     gene            1656..2102
                     /locus_tag="PK348_04510"
     CDS             1656..2102
                     /locus_tag="PK348_04510"
                     /inference="COORDINATES: protein motif:HMM:NF017020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HXK65507.1"
                     /translation="MVETTQGLDDIFTLIESKLKGFIVNYNAHNYRECVTALFHVAEG
                     LVRYVLATRGYFPISHEGIQVLLAQHFIKPGSVKKNVYNYLTNLYMRRKDADYKGFVS
                     FDSDDIKIYWQWVLEIVDEFQSFLDEIHKQKIDELVTEGKTLVLKK"
     gene            2243..3814
                     /locus_tag="PK348_04515"
     CDS             2243..3814
                     /locus_tag="PK348_04515"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003456284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HXK65508.1"
                     /translation="MAKNSELILSIDAGTQSIRAIVFNLQGNVVDIVKTPIEPYFSNY
                     PGWAEQDPEYYWKTLCTTTKKLFAKGKVKKDAIRGVTLTTQRDTVINVDKNGKPLRPA
                     IVWLDQRRADKSIVKLPWYIKAGLRLVNMLDAVEYFITEAEANWIRQYQPEIWEKTYK
                     FLLLSGFFTYRLTGDFVDSTGCQVAFIPFDYKTQDWAPASDLKWKLFPMSRDTLVRLV
                     KPTEVLGYITKKASNETGIPQGLPLIAGAADKACEVLGSGCLTPNIACLSYGTTATVE
                     TTNTQYVEIIPFFPPYPSAVPGSYNTEVMVYRGFWMLSWFVKEFGAREELQAKKMGIK
                     PEALFDRLVKEVPPGSMGLTLQPYWSPGVKIPGPEAKGAIIGFGDVHTRVHIYRAILE
                     GLAYALREGAERTQKRNKVPIEKLRVSGGGAQSDVAMQLTADVFNLPAERPHTFETSA
                     LGAAIDAAVGLKMYPDFKTAVSAMTRVGKVFYPNPKDVAIYDKLYKKVYKRLYNRLQP
                     LYQDIRDITGYPEKR"
BASE COUNT         1202 a          707 c          867 g         1088 t
ORIGIN      
        1 gaaaaaaagt ggcaaagaat gtaggcattc tcattgagct ggccaagcag tatagccttc
       61 ccataatcct taccgaacaa taccgcaagg gactgggacc cacaatcaag gagttgatgg
      121 atcgcctgga gggagtggtt gcatttgata agctggcttt tgattgcatg aaagataata
      181 ccattcgaga gcaggttacc aggataaaac gcaccaatat cattttgtgc ggcattgaaa
      241 cacatatatg tgtactcacc acggctctgt cactgcttaa agaaaattat aatgtcatca
      301 ttgcatcaga tgcggtgtgt tcgcgacgca ctcacgaatg ggaaatggca atacatgcac
      361 tcagacaggc aggtgcaata gtctatccca cggaaacaat atcatttatg attctggaaa
      421 aggcagggac agaacgtttc aaacaactat cgcccctgtt caagtagctt atggggtatc
      481 ccacagaact gtgtgggcat caaacacagg gccatcaatg caggcgcgtc tgtagccatt
      541 gatggttgcc acagcgcatc ctgagcaaag gccaacgccg cagccaaagt atcgttcaag
      601 tgaagcttca atgggtatgt gcgatagttc ccgggaaagg cgtttcatca taatttcagg
      661 gccgcaggtg tacacaatat caaacttgtg ttttgccaca atgtcttttg ccacatcaat
      721 gggattgccc ttaataccca agcttccatc atccgttgtt acaatacatg tgtgcgatag
      781 ttcctgatac ctatctagca aaaaaatgtg agatgctgaa cgcgaaccat agatataggt
      841 aacctgagta tttactctac gtagcatttt ggctaaataa tacagtggtg catttcccac
      901 accaccacca accagtaaag cctttttgcc agtaactact gaaaacnnnn nnnnnnttgc
      961 caaatggtcc aatacaatct acagggccag gatgcagggc aactatcctt tctgtgcctt
     1021 tgccaaccac acggatgaca atgtgtatgg tagtttggtc atggtcaaag atggaaaatg
     1081 gccggcgtat gagcggatca gtagatgtat ttacttttat gttaataaat tgtcccggga
     1141 tactctcttt agcatcacaa tgtaacgtca gcagataatg atcgtctgct accggttttg
     1201 tctcaataag cgtaatcata ctgcacctcg tgtaattata tacagtacac tatagcatgg
     1261 gcaaatctgg caatattaat ttttattatc ttgactttgc atagacaggg tgatacttgt
     1321 tttatcaggt aaacaggaat gattgttgca atgagtaaac ttacgccata tgaagaaaaa
     1381 ttgattaatg actttgtaga atatattacc agtgttccgt atgtcaatag tattgtagtg
     1441 tatggttccc gtagtaaggg tgtaagtaat gaatacagtg atgtggatat tgctgttgtg
     1501 atagataatt tacacaatag caaaaaagtg gaaaaattaa ttgagcaatg gaacattgat
     1561 aattcaccgg aaatactggt ccattttctt gttattgata ctaacaattt gcattcatca
     1621 gcaattggca aagaaattga aaaaggtgac actgtatggt tgagacaacg caagggttag
     1681 atgacatttt tactcttata gaaagtaaac tgaaaggttt cattgtaaat tacaatgctc
     1741 acaattaccg tgaatgtgtt acagcattat ttcacgtagc tgaaggcctt gtgaggtatg
     1801 tgcttgcaac acggggttat ttcccaattt cccatgaagg aatacaggtg ttacttgcgc
     1861 agcattttat taaaccaggt tcggttaaaa agaatgtata taattacctg actaaccttt
     1921 acatgcgaag aaaggatgct gattacaaag gttttgtttc ttttgatagt gatgatataa
     1981 agatatactg gcagtgggtt cttgagatag ttgatgaatt tcaaagtttt cttgatgaga
     2041 tccataaaca aaaaatagat gaacttgtta cagaggggaa aacgttggta cttaaaaagt
     2101 gatttttaga acaaattctt ataattttat gttgataaaa tatcactata cgtaatacaa
     2161 cgacattatg aataataatg tgtacatcct ttaaaagcga taggaatgca caaaataatt
     2221 atttgtacaa caggtgaaaa ctatggcaaa aaattcagaa cttattttat caattgatgc
     2281 cggaacacag tccataagag caatagtctt taatttacag ggaaatgttg ttgatatagt
     2341 aaaaactcca atagaaccgt atttcagtaa ttatcctggc tgggctgaac aggaccctga
     2401 atattactgg aaaacgttgt gcaccactac aaaaaaactt tttgcaaaag ggaaggtaaa
     2461 aaaggacgct atccggggcg ttacgttgac cacacaacgt gatacggtga ttaatgtgga
     2521 taaaaacggc aagccactga ggcctgctat tgtgtggctt gatcagcgcc gtgctgataa
     2581 gtcgattgtt aaattgccat ggtatataaa agcgggatta agacttgtaa atatgcttga
     2641 tgcggtagag tattttatca cggaggctga agcaaactgg atacgtcagt accagccaga
     2701 aatatgggaa aagacatata agtttttatt gttatcagga ttttttacat acagattaac
     2761 cggtgatttt gttgattcaa cgggctgcca ggttgcattt attcccttcg attataaaac
     2821 acaggattgg gcacctgcct ctgatttaaa gtggaagctt tttcccatga gcagggatac
     2881 tctggtgcgg ctggtgaaac ctactgaagt gttaggctat ataaccaaaa aagcttctaa
     2941 tgaaacggga ataccacagg gtttgcctct tattgccggt gcagctgata aagcatgtga
     3001 ggtgttaggt tcgggctgtt taacaccaaa catagcctgt ttgagctatg gcaccactgc
     3061 aacggtagaa acaaccaata ctcaatatgt tgaaatcatt ccgttctttc ctccatatcc
     3121 cagtgcagta ccaggatcct ataatacaga ggtaatggtg tacagaggct tctggatgct
     3181 gagctggttt gtaaaggagt ttggtgcacg tgaagaactt caggctaaaa agatgggcat
     3241 aaaacctgaa gcactgtttg acaggctggt aaaggaagta ccacctggtt caatggggct
     3301 gacattgcag ccatactggt cacccggtgt taagattcct ggtcctgagg caaaaggtgc
     3361 tatcattgga tttggtgatg tccacactcg tgtgcacatt taccgtgcaa tacttgaggg
     3421 attagcgtat gcactgcgtg aaggtgcaga acgcacacaa aagcgcaaca aggtgcctat
     3481 tgaaaaattg cgcgtttcag gtggaggtgc ccagagcgat gtagccatgc agctgacagc
     3541 tgatgttttt aaccttccag cagagaggcc acatacgttt gaaacatcag cacttggtgc
     3601 tgcaattgat gctgcagtgg gtttgaagat gtatcctgat tttaaaactg ctgttagtgc
     3661 catgacacgg gttggtaagg tgttctaccc caatcccaaa gatgttgcta tctatgataa
     3721 gctatataag aaagtttata aaagattgta caacaggttg caacctttgt atcaggatat
     3781 acgtgatata acaggctatc cagaaaaacg atagcatcac tatacgtgat acgtgcgctg
     3841 gaaagtatat ggaaactgta tcgggtgtac tatg
//
LOCUS       DAOSZP010000155         3868 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_18983_length_3868_cov_3.875164, whole genome shotgun sequence.
ACCESSION   DAOSZP010000155 DAOSZP010000000
VERSION     DAOSZP010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3868
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18983_length_3868_cov_3.875164"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..627)
                     /locus_tag="PK348_04520"
     CDS             complement(<1..627)
                     /locus_tag="PK348_04520"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HXK65509.1"
                     /translation="MKNKRIIPVIIVLVIVVGVTLYFEFVRYIGTNGKRIEGSGVIEV
                     TEIEVASKIAGRIVSIPFDEGEEVKQGDLIAKLAYDELSAQKLSALANLNNAEKNLKR
                     ARELFTTGSISQREFDNAVTAYNVAKGNYDYVMATIEQAVLYAPVSGVVLSRNLEVGE
                     LAFPGTSIMTLADLTKAWIKIYVNEKHIGLVKHGQKALVFVDSYPDKTF"
     gene            complement(617..2014)
                     /locus_tag="PK348_04525"
     CDS             complement(617..2014)
                     /locus_tag="PK348_04525"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HXK65510.1"
                     /translation="MKAKGFVIQVLSPIIFLLFISYAHAEQPLSLIDAIDIALKNSPE
                     YIMAQHRLQQADEKIAQAWGMLYPALESEASAMRQYAENGFMSLSDGQYDLKFVQVRF
                     GINPATFYNSLSQSYNNYKLAKEEVKKVKSSIEYNVIKSYFDVITANEIIRLRENSIA
                     VLQANVADVNQMFNTGTIPKFELLQAQVQLQSQEPLLIEARNNFSTALDLFNYYLGAK
                     NGTYTVIFEGFDSSIKEPHGYDIVDTLVEKALMHRPEVIQLTLTRDIVGDSIDAAQSV
                     YLWPTFSVAGYYGKSYLMPDAPQIQLPFPGQSIDLSAITGTRQWQTTWQVRVAATYRW
                     GALAPVDTVQGVEREQKEKLKEINQQILQLKQAIAIAVRADYGRLMTAYQSMVSQRKN
                     IETATEGLRVAKESYKAGVIKNADLLNAELQLTSARMGYIKAINDFWYAVAQLKKDTG
                     FDCTDLIFAEVHNEK"
     gene            complement(2011..2619)
                     /locus_tag="PK348_04530"
     CDS             complement(2011..2619)
                     /locus_tag="PK348_04530"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HXK65511.1"
                     /translation="MASSKDKILNAAIDEFSRYGLDGARIDRIAKRARVNKAMIYYHF
                     PGKEALYASILQELAQTIVDTISTSIAKVQETGNPLIIIEAYASHISNLDRRYFMIIM
                     RELASGGKFIKNIIAPTIIEPVTERIGNLFEAVKSKRINPKLHPQYTMISVAGAVIFY
                     NLMRQILQGTSLHALYFSDNHVHEYIANLLQLMSHGIIKEDI"
     gene            3307..>3868
                     /locus_tag="PK348_04535"
     CDS             3307..>3868
                     /locus_tag="PK348_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65512.1"
                     /translation="MKLRTLLILCVYFISFFECIVFGNLLAINITEIHGWKSLESDID
                     FLWENQHMYRYFQPNWNYPIAKEQVVERLETLLKKLDKLEAKNSRNIEYYLLKGDIAH
                     YLYNLDKTQYYKKALIMYEKAKTIEPRDYRPYWFIGQHEFLSGHNVKGYESFKTVIDV
                     IPPDKFHWAFWGDYSNCASIGMMPFNF"
BASE COUNT         1225 a          724 c          718 g         1201 t
ORIGIN      
        1 aaaggtttta tccgggtaac tatcgacaaa cacaagcgcc ttttgtccgt gtttaaccag
       61 cccaatatgc ttttcgttaa catagatttt aatccatgct ttagtaaggt cagcaagcgt
      121 catgattgat gtaccgggga aggcaagctc acccacctcc aggttacgtg aaagcaccac
      181 accgcttacc ggcgcataca gtactgcctg ctcaatggtt gccatgacat aatcgtaatt
      241 accttttgca acgttgtatg cggtaacagc gttgtcaaac tcacgctgcg atatagaacc
      301 ggtagtaaaa agttcacgtg ctcgtttcag gttcttttca gcattgttaa ggtttgccag
      361 cgcggacagt ttctgtgccg atagctcatc atatgctaac tttgctatta aatcaccctg
      421 tttaacttct tcaccttcgt caaaggggat ggaaacaatt ctgccggcaa ttttagatgc
      481 aacctcaatc tcggtgactt caattacacc ggagccttca atacgcttac cgtttgtgcc
      541 tatataacgc acaaactcaa aatacagcgt tacgccaact acaataacaa gtacaataat
      601 aacaggtatt atacgtttat ttttcattgt gcacctccgc aaatataaga tcagtacaat
      661 caaaaccggt gtcctttttt agctgagcaa ccgcatacca gaaatcattt attgccttaa
      721 tgtaacccat gcgcgctgat gtaagctgca gttctgcatt gagtagatca gcgtttttta
      781 taacaccagc cttatagctt tcttttgcaa cacgtaatcc ttctgtagca gtttcaatat
      841 ttttacgctg ggataccatg ctctgatagg cagtcatgag cctgccgtaa tcagcacgaa
      901 cagcaatagc aattgcctgt ttaagctgca atatctgctg gttaatttct tttagctttt
      961 ctttttgttc acgctccaca ccctgcacgg tatcaacggg agccagtgca ccccatctat
     1021 aggttgcagc aacgcgcacc tgccaggtag tctgccactg cctggtcccc gttattgcag
     1081 ataagtctat gctttgcccg ggaaaaggta gctgaatctg tggagcatct ggcataaggt
     1141 aacttttgcc ataatatcct gctacactga atgttggcca caggtacacc gattgtgcag
     1201 catcaatact atcaccaaca atatctctgg ttaaagttag ctgaattact tctggcctgt
     1261 gcataagagc cttttcaacc agcgtatcaa caatgtcata tccatgtggc tcttttatag
     1321 aactatcaaa cccttcaaaa ataacggtat aagtgccgtt tttagcaccc aggtaatagt
     1381 taaaaaggtc aagtgcagtt gaaaagttat tacgtgcctc aatgagtaaa ggttcctgac
     1441 tttgcaactg cacctgagcc tggaggagtt caaattttgg gattgtgcca gtattaaaca
     1501 tctggttgac gtcagctaca ttagcctgta atacagcaat tgagttttct ctgagtctta
     1561 taatttcgtt agcagtgatg acgtcaaaat agcttttgat gacattgtat tcaatgctgg
     1621 attttacttt tttaacttct tcttttgcaa gtttgtaatt gttgtagctt tgcgatagtg
     1681 aattataaaa ggtagcggga ttgataccaa agcgcacctg cacaaatttt aaatcatact
     1741 gaccgtcaga aaggctcata aatccatttt cagcatactg tcgcatggcg cttgcttcag
     1801 attctaatgc cggatacagc attccccatg cctgggctat tttttcgtca gcctgctgta
     1861 acctgtgttg tgccatgata tattccgggc tgtttttaag tgctatatca attgcgtcaa
     1921 tcagtgaaag tggctgctct gcatgtgcgt atgaaataaa aagcaaaaaa attatgggcg
     1981 atagtacctg tataacaaaa ccttttgcct tcatatatcc tccttgataa ttccatgtga
     2041 catcaattgt aagagattgg caatatattc atgtacatga ttgtcactaa aatagagcgc
     2101 atgaagagag gtgccctgta aaatctgtcg catgagatta taaaagataa cagcaccagc
     2161 gaccgaaatc atggtatatt gtggatgcag ttttggatta atacgtttgc ttttcacagc
     2221 ctcaaaaaga ttgccaatac gctcagtaac aggctcaata atagttggag caatgatatt
     2281 ttttataaat ttcccgccac ttgcaagctc acgcatgata atcataaaat agcgcctgtc
     2341 taaattgcta atatggctgg catatgcttc aataatgata agtggatttc cggtttcctg
     2401 cacctttgca atactggtag atattgtatc aacaatagtc tgtgcaagtt cctgtaaaat
     2461 tgaagcatac agtgcttctt taccggggaa atgataataa atcattgcct tgtttaccct
     2521 ggctctttta gcaatgcggt caatgcgagc accatccagc ccataccggg aaaattcatc
     2581 aattgctgca ttcagtattt tgtcttttga agatgccatg actatcttat gttaactaac
     2641 tgtttagtta gcatattggc atctgggtta aaaaaatcaa gattttttta tttattcact
     2701 taaatatatt ttttaataga atgattacaa aagtttatga taagtttctg atatatgggg
     2761 tgcatcaaat agcaataagc aaattttaaa tgcagattca agaataaagt gcaccacaca
     2821 atagaaaaca tgtagcatca tacacagcac atcctttaaa gtgggtgttc caacaacaaa
     2881 atctaaaaag caggtatgta tgaggcatta ctcatataca atcaggagaa agaattgtca
     2941 ttctaaagat tcacaggaaa aagaatgtgc catcagcata aaaagtataa ctatataaag
     3001 aaataagtga aaagacaaac caagatgttt tttaaaaaca gcaaaggtgg ataaaagcaa
     3061 aagtaggggg caatagtata acaataatga taaatacacg tccaggtaaa tctaaactat
     3121 acaaatcttt gtatgagttt tacagagggg actacatggt gcactgtaaa tgtgaatttg
     3181 caatgaaaaa ttatagtgaa aaaaattttt atttgatttt ataaatggta gtattgtacg
     3241 ataaacaaaa atataaaata tataaattgt ttaaaattat atgatacaaa atatatgagg
     3301 tatacaatga aattaaggac tttattaatt ttatgtgtat attttatatc tttttttgaa
     3361 tgtattgtat ttggtaatct tttagctata aatattacag aaatacatgg atggaagtca
     3421 cttgaatcag atattgattt cctgtgggaa aatcaacata tgtatcgata ctttcagcca
     3481 aattggaact atccaattgc taaggaacag gttgttgaac ggttagaaac cctattaaag
     3541 aaattagata aactagaagc aaaaaatagt agaaacatag aatactacct tttaaagggt
     3601 gatattgctc attatttata taaccttgat aaaacacagt attataaaaa agctcttatc
     3661 atgtacgaaa aagctaaaac aattgagccg cgcgattata gaccatattg gtttattggg
     3721 cagcatgaat ttttatcggg acataacgtc aaaggatatg aaagctttaa gacagttatt
     3781 gacgttattc caccggataa atttcattgg gcattttggg gcgattattc caattgtgct
     3841 tccattggca tgatgccatt taattttt
//
LOCUS       DAOSZP010000156         3867 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_18991_length_3867_cov_2.816894, whole genome shotgun sequence.
ACCESSION   DAOSZP010000156 DAOSZP010000000
VERSION     DAOSZP010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3867)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3867)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3867
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18991_length_3867_cov_2.816894"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1090)
                     /locus_tag="PK348_04540"
     CDS             complement(<1..1090)
                     /locus_tag="PK348_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020772934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-associated domain-containing protein"
                     /protein_id="HXK65513.1"
                     /translation="MTKPLIVQSDNTLLLEVDNPLYEEVRDAILPFAELEKSPEHIHT
                     YRITPLSLWNAASAGLTAKEIVERIERYSRYTVPAIVITTIKEQMDRYGLLTLIKRGE
                     NLILQSDDVHIIREIATHKQIHKFIEEQLSEYEILVKPNNRGHIKQALIKIGFPVEDK
                     AGYVDGDPCPFELRSVTASGIQFTIRDYQKSAIDSFYRSGSDEGGSGVVVLPCGAGKT
                     IVGIGIMHLYQTKTLICVTNTVALRQWIDELLDKTTLTKDMIAEYSGDVKDIKPVTIA
                     TYNILTYRRKKEDEFKHFGLFTQNNWGLIIYDEVHLLPAPVFRMTAEIQSKRRLGLTA
                     TLVREDGLETDVFSLIGPKKFDMPWKLLE"
     gene            complement(1094..1525)
                     /locus_tag="PK348_04545"
     CDS             complement(1094..1525)
                     /locus_tag="PK348_04545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65514.1"
                     /translation="MIKVILSQTFNASAAEVFAALDHINKLARLKTDLRNYRIARDEE
                     GLQLIDVDVRFIVKQFPLRLKYTAVPNKYCELKMLKGALKQYEYRYTVMGDNVSATVT
                     VECSIRLPFRYFLVTPILRAILKRRIKKELLLLEKILHTTL"
     gene            complement(1784..3166)
                     /locus_tag="PK348_04550"
     CDS             complement(1784..3166)
                     /locus_tag="PK348_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXK65515.1"
                     /translation="MEYNVETLPLWKKIIYALGQLGWSLASFSVGNLLIYFYLPPESA
                     DKAIFPAYIYQGYVIGVLTIIGLIYAFGRLWDAITDPIIATMSDRSRSPFGRRRKFLA
                     IGAFPFAILGMLAFMPPVDTLSTWNAVWLFVVVTLFYWFMTMYVTPFFAWLSELGHNP
                     NERLFLSTLISITWAIGFAIGSQAVALQSLFEKSYGYSSTKAFQVVMIIFSGVSLVLM
                     YLPVIFIDERRYCEPNVSNEGTFEAVRSAFKNKNFLFFTISDLAYWVALTFITTGLVY
                     YVTVLLELDKAVYSQYMIILFALSFIFYVPVNLIAQKIGKKKLLVFAFVLYSSVYVVA
                     SMLGWFPIDKNVQGLIIIVMTSLPLAIFGILPNAIIADIAEADGIQTGNFKAGIFFGA
                     RTFMSKMGQMIAALIFPSLILLGKTPQNDIGVRLTCVVAFFFCIAGLILFLKYDEKKV
                     LEILATKEKV"
     gene            complement(3557..>3867)
                     /locus_tag="PK348_04555"
     CDS             complement(3557..>3867)
                     /locus_tag="PK348_04555"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HXK65516.1"
                     /translation="GGIYIELFKDVSIRLAPVTKAEALEMIRELKAARLFEGFRGKEP
                     LDANAFSDVIVTLSHAIASIEEISDIECNPVMVYSHGCMAVDARIFLQTQKVASPTSW
                     "
BASE COUNT         1245 a          790 c          659 g         1173 t
ORIGIN      
        1 cctctaacaa cttccagggc atatcaaatt tttttggccc aataaggcta aacacatctg
       61 tttcaagtcc atcctctcgc accagtgtgg cagtaagtcc caaacggcgc tttgattgta
      121 tctcagcggt catacggaat accggcgccg gcaaaaggtg cacttcatca taaatgatta
      181 agccccagtt attctgagta aacagaccaa aatgtttgaa ttcatcttct tttttgcgtc
      241 tataggtcaa aatattatag gtggcgatag ttactggttt aatgtcttta acgtcaccag
      301 aatactcggc aatcatatct ttagttaatg tagttttatc cagtagctca tcaatccact
      361 ggcgcagagc aacagtgttg gttacacata tcaatgtttt tgtttgatac aggtgcataa
      421 tgccaatacc aacgatggtc ttccctgcac cacagggcaa caccaccaca ccgcttccac
      481 cctcatcact tcctgagcga tagaaactat caatagcact cttctgataa tcgcgaattg
      541 taaactggat gccgcttgcc gtaacacttc gcaactcaaa cgggcaggga tcaccatcaa
      601 cgtatcctgc tttatcctca acgggaaatc caatttttat cagcgcttgt tttatatgac
      661 cgcgattgtt aggctttacg agtatctcat attcagaaag ttgctcttca ataaatttat
      721 gaatctgttt atgggtagct atctcccgta taatatgcac atcatcagat tgcaggataa
      781 ggttttcacc acgctttatc agggtaagca aaccatagcg gtccatctgc tcttttatgg
      841 tggtgatgac tattgcaggg acagtatagc gcgagtatcg ttcaatacgc tccacaattt
      901 cttttgcagt aagacctgct gaggctgcat tccacaatga taacggcgtt atgcggtagg
      961 tgtggatatg ctcagggctt ttttcaagct cagcaaatgg aagtatagca tcccgcacct
     1021 cttcatacag gggattatct acctcaagaa gtaaagtatt gtcgctctgg acgatcagtg
     1081 gtttggtcat gtgttatagt gtagtgtgta atattttttc aagtaataat aattcttttt
     1141 tgattctgcg ctttaatata gctctaagaa tcggtgtaac aagaaaatat cggaacggca
     1201 acctgattga gcactcaact gtaaccgttg cggacacatt atcacccata acggtatatc
     1261 tgtattcgta ctgcttcaaa gctcctttga gcattttgag ctcacagtat ttattgggaa
     1321 cagcagtata tttgagcctt agtggaaatt gtttaacaat aaaacgtaca tccacatcaa
     1381 taagctgtaa tccttcctca tcacgagcta tgcgatagtt ccgtaaatca gtcttcaacc
     1441 gtgccagctt attaatatga tccagtgctg caaaaacttc ggcagcagaa gcgttaaagg
     1501 tttgtgatag aataactttt atcatacttt tgctatttcc ctcaaatgca aaatgtattg
     1561 ttgatggtat atgaatattt gtcaaatatt taataatctt atttagaggg aaaagggggt
     1621 tgtatgcccg atgttatttt aatcacagtt aaaaaattct ataagcacac ctataaaatt
     1681 gatgttcata ggaacaattt tttgtaaagg taataaacaa ggggatacgg cgttgatgtt
     1741 tgaatatcct tatcccctta tgaaaaataa cattataaaa atatcacact ttttcttttg
     1801 ttgccagtat ttccagaacc ttcttttcat catatttcaa gaaaagaatt aatccagcaa
     1861 tacagaagaa aaatgccaca acacacgtca atcgtacacc tatatcattc tgtggtgttt
     1921 tgcccagtaa tatgagtgac gggaatataa gtgcagcaat catctgcccc attttggaca
     1981 tgaaggtacg tgctccaaag aaaattccag ctttaaaatt acccgtctgt attccatcag
     2041 cttcagcaat atcagcaatg atggcattgg gtaaaattcc aaaaattgct aatggtaaag
     2101 atgtcataac aataatgatt aagccctgaa catttttatc gattgggaac catcccaaca
     2161 tagatgccac aacgtacacc gatgaataca gtacaaatgc aaataccagt agctttttct
     2221 tcccaatctt ctgtgctatc aagtttaccg ggacatagaa tataaaactt aatgcaaata
     2281 aaataatcat atactgagag tatacagctt tatccagttc aagtaataca gttacatagt
     2341 acacaagacc agttgtaatg aatgtaagtg caacccagta agccaaatct gagatagtaa
     2401 aaaataagaa atttttattt ttaaaggctg aacgtactgc ctcaaaggta ccctcattgg
     2461 aaacattggg ctcacagtat cgccgttcat caatgaaaat aaccggtaaa tacataagta
     2521 ccaatgatac gccactgaaa attatcatta caacctgaaa tgcttttgtg gaactgtaac
     2581 catagctttt ttcaaacaat gactgtaatg caactgcctg tgagccaatt gcaaatccta
     2641 tagcccaggt aatagaaatc aatgtactca aaaataaccg ttcattggga ttgtgaccca
     2701 attcagaaag ccaggcaaaa aaaggtgtta catacatagt cataaaccag taaaacagtg
     2761 ttacaaccac aaaaagccat acagcattcc atgttgaaag agtatccaca ggtggcataa
     2821 acgctaacat tcccagaatt gcaaagggaa atgcgccaat ggctaaaaat tttctccttc
     2881 tcccaaacgg tgaacgtgac ctatcggaca ttgttgctat aatcggatca gttatagcat
     2941 cccatagcct tccaaaagca tagataagcc caataattgt taacactcca atcacatagc
     3001 cttgatagat atatgctggg aatatggctt tatcagctga ctcaggtggt agataaaaat
     3061 atattaataa attaccaaca ctgaatgaag ctaatgacca ccccaactgc cctaaagcat
     3121 agatgatctt tttccacaac ggtaaggttt caacgttgta ttccatactg ttttctcctt
     3181 gcaggtaata tcatcctttt attcattcat aaggaagaaa gataatatat cgataacgat
     3241 attattaaac cttaaattaa aatcaatcct tttttattga atacatataa ttattttatg
     3301 gcattttgta aatattgtac agaataaaat tttcaaatgt cttggggatt tactgtcata
     3361 ttaatatcca gaatcaagcc tttataaccg aaagccggga ttcagaataa ctttttaaaa
     3421 aaatatttta ttagtttatg gtataaccat cactgcatgc gtacccacct tttgaatgaa
     3481 attgttgcaa tataaaaaaa cccccaccct ttatattttt aaggcgggtt tattctattt
     3541 ttcagttaaa attattttac catgacgttg gtgatgcaac tttttgcgtt tgcaaaaaga
     3601 ttcgcgcatc aacagccatg cacccatgcg aatacaccat aactggattg cattcaatat
     3661 ctgaaatttc ctcaattgaa gctatagcat gacttaatgt aacaatgaca tcactgaatg
     3721 cattggcgtc aagtggctcc ttacctctga acccttcaaa taaccgtgct gcttttaact
     3781 cccgtatcat ttccaatgct tctgcctttg taacaggagc aagtcgtata gatacatcct
     3841 tgaatagctc aatataaata cctccca
//
LOCUS       DAOSZP010000157         3866 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_18996_length_3866_cov_3.581475, whole genome shotgun sequence.
ACCESSION   DAOSZP010000157 DAOSZP010000000
VERSION     DAOSZP010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3866
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18996_length_3866_cov_3.581475"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..152)
                     /locus_tag="PK348_04560"
     CDS             complement(<1..152)
                     /locus_tag="PK348_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015336374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXK65517.1"
                     /translation="MNKKAVALYSGGLDSLLACAIIQKQGIEVIGLHCILPSVDPAIN
                     PDSLKAS"
     gene            complement(156..1379)
                     /locus_tag="PK348_04565"
     CDS             complement(156..1379)
                     /locus_tag="PK348_04565"
                     /inference="COORDINATES: protein motif:HMM:NF015418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HXK65518.1"
                     /translation="MITPDEAIKIILSHIQPLPVEKVHFTNASGRICADDMYATENIP
                     YHTNSAMDGYAIVASDTLNASNNNPVTLPIAGEYQAGIIHNPIPIKSGEAIRIMTGAP
                     IPPGADAVVEKEATTEDNNQVTIFRRVKEGENIRQAGEDIKKGDCIVSRGTKITPAIV
                     GLLASTGNVTVTVAQKPRVAILATGDEIYEPGTPLKEGIVINSNAYTLMSLVQEYGGI
                     PHYVGIARDSMEASKEALTQALSCDIIISSGGVSEGKYDFMISAIAELGFTLLVERIA
                     MKPGKPCIFAHRGNTLYFGLPGNPVSSMVSFIQFVRPAMLAMQGAKALQKPLIKAICQ
                     EPIKRKNDGRTHFIRGIVSFEGGQASVRITGPQGSGILRSMALANCLIILPPDRLSVS
                     QGEHVDVQLIHHPEV"
     gene            complement(1601..>3866)
                     /locus_tag="PK348_04570"
     CDS             complement(1601..>3866)
                     /locus_tag="PK348_04570"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HXK65519.1"
                     /translation="IQQLITNAPDQLWDEFHAYLKGINSFKKFFDNFSFIKNHTQALN
                     DYFKRFASGIPDNDYILNRLKVGIQLEKYDVNPILFSTSYGNVYSYLLEYIKNSINDD
                     SLLAPVVKAYMKIVFLDLALILYAYFFVKDKKLSESKKELAHVTRSYRLIHEINDLII
                     RTNDEKALFENTVKILDALGNFDLAWIGIVDNDLKVVPVASCGKTEYLDNIFISVDPA
                     IPEGNGPVGTAIREKTTVRINYPYEDPRYAPWKERAHKYGFKSIISMPIVPDEQHDAI
                     GAITLYSCHENFVSLGEAAMLQEVANDLSFAIRNIRRDKEYEAILFTDTLTGLGNVHY
                     FTSMLSSQMKVASQQNVKLAIVTIDIDNFTAINNSIGYLQGDKILQEFGKRISQLLHN
                     SHLVARSSDEFYTIFPFTDDMELINFIDDLKRLSMTPFDDISNDLMVTYSIGIAVFPG
                     DGNNADDVVHKSRVALSEAKKIGYGTVCYYSEQLFQKNIKKISLQQELRAALKNNEFE
                     LYYQPTINLTTRQISGLEALIRWHHPKRGMLAPSHFISVLESSDIIIQAGRWIIAEVS
                     RQVQITPQLQQHGFTVSFNVSPAQFYDSNFIVDVIKSVKSSMINPSHLQIEVTENLLM
                     SDAAEVINTCNALQQFGINIAIDDFGTGYSSLAYLKKLPLSTLKIDSSFVQGLPDNRD
                     DVEIVKTIVLLAQSLGKRTIAEGVETREQLAFLTGLGVDEAQGYYFSKPMPMSDCMEY
                     ITNFNHEKYFWPKR"
BASE COUNT         1128 a          742 c          767 g         1229 t
ORIGIN      
        1 gaggctttaa gactatcagg gtttattgca gggtccacag aaggcaaaat acaatggaga
       61 ccaataacct caatgccctg tttttgaata atggcacaag ctaaaaggct atccaggccg
      121 cctgaatata gtgctactgc ttttttgttc atagattaca cttcgggatg atgtataagc
      181 tgaacatcaa cgtgttcacc ttgtgaaaca ctgagcctgt caggtggcag tataatgagg
      241 caatttgcca gtgccataga acgcaagatc cctgaaccct gtggtcctgt tatgcgtaca
      301 gacgcctgtc caccttcaaa cgatactatc cctcggatga aatgcgttcg gccatcgttt
      361 ttacgcttta taggttcctg gcatatagct ttaataagcg gtttttggag agcttttgca
      421 ccctgcatgg cgagcatagc aggccgcaca aactgaatga acgacaccat ggacgataca
      481 ggattgcccg gcagtccaaa atacaatgtg ttgccccgat gggcaaatat gcaaggttta
      541 cctggtttca tggcaatgcg ttcaactaaa agcgtaaatc ccagttcagc aatagcactg
      601 atcataaaat catatttacc ttctgaaacg ccgcctgaag aaataatgat atcgcacgat
      661 agtgcctgtg tcagtgcttc tttgcttgct tccatgctgt cacgggcaat acctacataa
      721 tgcggtatgc caccatattc ctgcaccagt gacatcagcg tgtatgcgtt tgaattaatg
      781 acaatgcctt ctttaagggg ggtaccaggt tcgtaaatct catcgcctgt tgccagtatg
      841 gcaacacggg gtttttgggc gacagttact gtaacattgc ctgttgatgc taaaagacca
      901 acgattgcag gtgtaatttt tgtcccccgg ctaacgatac aatcaccttt tttgatatct
      961 tcaccagcct ggcgaatgtt ttccccttct tttacccgac gaaatattgt cacctgatta
     1021 ttatcttctg ttgtagcttc tttttcaact acggcatcag cacctggagg tatgggagca
     1081 ccagtcatga tgcggatagc ctctcccgac tttattggta tgggattgtg aataattcct
     1141 gcctggtatt caccggcaat gggcaatgtg acaggattgt tatttgaggc attgagtgta
     1201 tcactggcta ctatcgcata gccatccatg gcagaattgg tgtgataggg aatattttct
     1261 gtagcataca tatcatcagc gcagatgcga cctgatgcat tggtaaagtg aaccttttca
     1321 actggtaatg gctgtatatg tgacagtata atcttaatgg cttcatcagg tgtaatcatc
     1381 agtacctccc agaatggcaa aataaacact gtataagtaa cagtatactg ctttgaatgg
     1441 tgtttacaat tttttaaatg atggtagtgt ttatatcata ttaatggatt gtatgcgtca
     1501 aggaatatta tgtaatgaaa gtcatacctg attaaatgat ttttcatgaa aagggaattt
     1561 gaatcaagat atggttttgg aaaaattaaa cgagaatgca ttaccgtttt ggccagaaat
     1621 atttttcatg attaaaattt gtaatgtatt ccatgcaatc actcatgggc ataggctttg
     1681 aaaagtaata cccctgagct tcatcaacac ctaaacccgt aaggaacgcc agttgttctc
     1741 tggtttccac gccttcagct attgttcgtt tgcccagtga ctgagccaga agaacaatag
     1801 ttttaactat ctctacatca tccctgttat caggcaatcc ctgtacaaac gatgaatcta
     1861 ttttgagggt actcaaaggt agttttttta aatatgcaag cgacgaatac ccggtaccaa
     1921 agtcatcaat ggcaatatta ataccaaatt gctgcaaagc attacaggta ttaatgactt
     1981 ctgctgcatc ggacataagt aaattttcgg tcacttctat ctgcagatgt gaagggttaa
     2041 tcatggaact ttttacagac tttatgacat caactataaa attggaatca taaaattgtg
     2101 ctggtgatac gttgaacgaa acggtaaatc catgctgttg taattgtgga gtaatttgca
     2161 cctggcggct tacttcggca ataatccacc tgccagcctg aataatgata tctgatgatt
     2221 ccagtacaga aataaagtgt gagggtgcca gcataccccg tttggggtgg tgccagcgta
     2281 taagggcttc caggcctgaa atctgtcttg tagtaaggtt aattgttggc tgataatata
     2341 actcaaattc attatttttt aatgctgccc tgagttcctg ttgtagtgaa atctttttta
     2401 tatttttctg gaatagttgt tcagaataat agcatactgt accatatcct atcttcttgg
     2461 cttcagaaag tgcaactctg gatttatgta caacatcatc agcattgttt ccatcacccg
     2521 ggaatacagc aataccgata gaatacgtta ccatgaggtc attgcttata tcatcaaatg
     2581 gtgtcattga caatcgtttg aggtcatcaa taaaattgat caattccata tcatcagtaa
     2641 aggggaatat tgtatagaac tcatcactac tgcgcgccac caggtgtgaa ttgtgtaaca
     2701 gctgggaaat tcttttgcca aattcctgta atattttgtc accctgaaga tagcctattg
     2761 aattattgat tgcagtaaaa ttgtctatgt caatggttac tattgccagt ttaacatttt
     2821 gttgtgatgc aactttcatc tgtgaagaga gcattgaagt aaagtaatgt acattaccta
     2881 atccagtaag ggtatcagta aaaagtatag cctcatattc tttatctctt ctgatattgc
     2941 gtatggcaaa ggacaggtca tttgccactt cctgaagcat tgctgcttcg ccaagtgaaa
     3001 cgaaattttc atgacatgaa taaagtgtaa tggcacctat tgcatcatgt tgttcatcag
     3061 ggacgatagg catagaaatt attgatttaa atccgtactt atgtgccctt tctttccatg
     3121 gtgcataccg tggatcttca taagggtaat ttatccgaac ggtagttttt tctcgtattg
     3181 cagttcctac cgggccgtta ccttctggaa ttgcagggtc aactgatata aaaatattat
     3241 caagatattc agtttttcca caagaagcta ccggtacaac tttgaggtca ttgtcaacaa
     3301 taccaatcca tgccagatca aaattgccaa gtgcatcaag gatttttaca gtattttcaa
     3361 acagggcttt ttcatcattt gtacggatga taaggtcatt gatttcatgt attaaccggt
     3421 atgatctggt tacatgagca agttctttct ttgattctga aagtttttta tcctttacaa
     3481 aaaaatatgc ataaaggatc aatgctaaat caagaaaaac tattttcata tatgccttta
     3541 ctactggcgc taacagtgaa tcatcgttta tgctattttt tatgtattcc agaagatagg
     3601 aatatacatt tccatatgaa gttgaaaaga gaattggatt aacatcatat ttttcaagtt
     3661 gtattccaac ctttagcctg tttaatatat aatcattgtc aggtatacct gatgcaaaac
     3721 gcttaaaata atcattaaga gcctgtgtgt gatttttaat aaatgaaaag ttgtcaaaaa
     3781 atttcttgaa actgtttatt ccctttaaat aagcatggaa ctcatcccac aactgatcag
     3841 gtgcattggt gatcaattgt tggatt
//
LOCUS       DAOSZP010000158         3861 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_19032_length_3861_cov_3.685497, whole genome shotgun sequence.
ACCESSION   DAOSZP010000158 DAOSZP010000000
VERSION     DAOSZP010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3861
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19032_length_3861_cov_3.685497"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(143..976)
                     /locus_tag="PK348_04575"
     CDS             complement(143..976)
                     /locus_tag="PK348_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012610531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXK65520.1"
                     /translation="MYPDLKNKNAVITGAGKSTGIGFAIARELAKNGANIIIADLGKG
                     TIGDTTMMAGQLSQMQQLAAELQKEYSVTAQALLCDVTNKESINEFAVKVSELFDSVH
                     ILCNNAGATFGVPSAIHTYDDEAWLKTVDVNLHGTFRVSKAIVPLMKQGGSIINTASR
                     AGKFPAMFNGAYSVSKAGVIMLTKVMAKELAGAGIRVNAICPGQINTDLERWRFELEA
                     KVFNTTPEEREKLMCQSIPLNRIGTPEEVARLVVFLASEASSYITGQAINVCGGQLME
                     L"
     gene            1132..1464
                     /locus_tag="PK348_04580"
     CDS             1132..1464
                     /locus_tag="PK348_04580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65521.1"
                     /translation="MTTIVVNYFINNLTITMSEHNQSNRIKWKYMQKAPIPSTIKQLL
                     WDEQTVAPLEKIIMRVLQYGNYDEIKYIYGMYPEEVTEIVNRYTGLRRGVKFWVVYWN
                     KLYGHTNN"
     gene            1445..2011
                     /locus_tag="PK348_04585"
     CDS             1445..2011
                     /locus_tag="PK348_04585"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HXK65522.1"
                     /translation="MDIPIINLAQKIQSYFPKFYLAGGTAIMIKYNHRISYDLDFFSF
                     RAFSYTRLSKKMINLFIGHINRWERMEDNIDFFINDIKVSFVFFPFENIEKTEKINNI
                     TIASDIDLFLNKIYVAGRRIDPKDPFDAAFLYSKNQWDKVYIEECFGKKFHGQSYKIY
                     LGAICSFDDYGDLPDWVKDVLLSLTSPA"
     gene            complement(1995..>3861)
                     /locus_tag="PK348_04590"
     CDS             complement(1995..>3861)
                     /locus_tag="PK348_04590"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HXK65523.1"
                     /translation="LMAMMYVPEIFSHWVAALLSMPVFVFISFHIFVHAYHALKNRVL
                     SMDVMYAMGTGVSFVASVCATFGILPHEFVVYETAIFLATFLNIGKYLEDRARSRTSG
                     TIKKLLSLKPDTATVIRDNNEIEIKTEDVVVGDSIIVKPQSRVPVDGVIIKGAGYIDE
                     SMVTGESVPVYKKEGDRVIGGTLNKNNLLVIQAQVLGSDSVLSRIISLVQQAQESKPA
                     MQRFADRVVGYFIPVILIIAFVASVLWYVFTGNASFAFQVFVAVIVIACPCALGLATP
                     TAVTVGLGRGAELGILIKNAEALEKASNVTAVVFDKTGTLTTGKLTVIDVVALNEQHD
                     ELISLCATLERYANHPVGDAVVQYARDNGVLFKDAEEVKVFEGKGVMGTIDGRNIVAG
                     SLAFIKEGVDTGAIEHHIIVFEKQGKTVLVVADSKPLGIITLADKPKQEARVTLQRLK
                     TKGKKTIMITGDNRQAADAIAKEVGVDDVYSDVTPEAKMNIVMKLQNNGDVVAFVGDG
                     INDAPVLAQADIGIAMGAGTDVAIEAGDVVLLRNRLADVVTYFDLAVKVMKRIKQNIF
                     WAFAYNMLLVPVAAGILYPFTGFLIKPEFAGAAMALSSVTVVTLSLALKRFKPGK"
BASE COUNT         1227 a          859 c          634 g         1141 t
ORIGIN      
        1 tattgtaatt gatgagtttt ctaaatgttc atagatgggg gtgatatgtc ccccacttaa
       61 cgaaaagata tagtcaacct gtttatccag taatgcttca accacaagtt ttgcaccatt
      121 agccatacaa tcctccatac tgttataatt ccattaattg cccgccacag acatttattg
      181 cctgccctgt aatatatgaa gacgcctcag aagctaaaaa tacaacaagc ctggcaacct
      241 cctcaggtgt gccaatcctg ttaagcggga tactctgaca catgagcttt tcccgctctt
      301 ctggtgttgt gttgaatact tttgcttcta attcaaaacg ccagcgttct aaatctgtat
      361 ttatttgccc tggacaaatt gcattaaccc gtataccagc accagcaagt tcctttgcca
      421 tgaccttggt gagcataata acgcctgcct tggaaactga ataagcaccg ttaaacattg
      481 cgggaaattt ccctgcccgt gaagccgtat tgattataga accaccctgt ttcataagcg
      541 gtacaatggc cttagaaacc ctgaatgttc catgcagatt gacatcaact gttttaagcc
      601 aggcttcatc atcataggta tgaatagcac tgggaacacc aaacgttgca cctgcattat
      661 tacacaggat atgtacagaa tcaaacaatt ctgaaacttt gactgcaaat tcattaattg
      721 actctttatt ggtaacatca cagagtaatg cttgtgctgt aacggaatat tctttttgta
      781 attctgcagc caactgctgc atctggctta attgaccagc catcatcgtt gtatcaccaa
      841 tggtgccctt acctaaatca gctataatga tattagctcc attttttgct agttcgcgag
      901 caatagcaaa accaatccca gttgacttac cagcaccggt gataaccgca tttttatttt
      961 tcagatcagg atacatgaca acctccaatt aatcgaaaat gacatatcat attattttat
     1021 ttatgtcaat aatgaattta tcaattactt caatggaagt tacaaaaagt aacggtgaac
     1081 ctgttagtta ttaaaaactc ttacaaaaat ataattgcaa attgtaagca catgactaca
     1141 atagtagtca attattttat caataatcta actattacaa tgagtgaaca taaccaatca
     1201 aatcgcataa aatggaaata catgcaaaaa gcccctatcc caagtactat aaaacagctt
     1261 ctgtgggatg aacaaactgt tgcaccactt gaaaaaatta taatgagggt actacaatat
     1321 ggtaattatg atgaaataaa atatatttat ggaatgtatc ctgaagaagt aactgaaata
     1381 gttaatcgtt atactggttt gcggcgaggg gtaaaattct gggtggtata ttggaacaaa
     1441 ttgtatggac ataccaataa ttaacttagc tcaaaaaata cagagttatt tcccaaagtt
     1501 ctatcttgcg ggcggtacag ccatcatgat taagtacaat caccgtatta gttatgatct
     1561 ggattttttc agttttcggg ctttttcata tacaagacta tcaaagaaaa tgataaattt
     1621 atttataggc catattaata ggtgggaacg aatggaagac aatatagact ttttcataaa
     1681 cgatatcaaa gtatcatttg ttttttttcc ttttgaaaat atagaaaaaa ctgaaaaaat
     1741 taataatatt accattgcaa gtgatataga tcttttttta aataaaatct atgtagcagg
     1801 gagaagaatt gatccaaagg atccctttga tgctgctttc ctgtattcaa agaatcaatg
     1861 ggacaaggtt tatatagaag aatgttttgg aaaaaaattt catggccaaa gctacaaaat
     1921 ttatctaggt gcaatatgta gttttgatga ttacggtgat cttcctgatt gggttaaaga
     1981 cgtcttactt tcccttactt ccccggctta aatcttttca gtgcaagtga caacgtaacc
     2041 acagtaactg aactcaatgc cattgcagcg cctgcaaatt ctggtttgat taaaaatcct
     2101 gtaaatggat acagtattcc tgctgcaaca ggtaccagta gcatattgta tgcaaatgcc
     2161 cagaatatat tctgcttaat ccttttcata acttttactg ccaaatcaaa atacgtaacc
     2221 acatcagcta acctgtttcg caataacacc acgtctcctg cttcaatggc aacatctgta
     2281 cctgcaccca tggcaatgcc aatatcagcc tgcgctaaca ccggtgcatc attaatgcca
     2341 tctcccacaa aagccacaac atcaccattg ttttgaagct tcattacaat attcatcttt
     2401 gcttcaggag taacatcaga atatacgtca tcaacaccaa cttctttagc tattgcatcg
     2461 gctgcctgcc tgttgtcacc ggtaatcatt atcgtctttt tccctttagt tttaagcctt
     2521 tgtaatgtta ccctggcttc ctgctttggc ttgtcagcaa gagtaataat acccagtggt
     2581 tttgaatctg ctacaaccag tacggtttta ccctgttttt caaacacaat aatatggtgt
     2641 tcgatagctc cggtatcaac cccttcttta ataaaagcca aactgccagc aacaatattt
     2701 cttccatcta tcgtacccat tacacctttg ccctcaaaaa ccttcacttc ctctgcatcc
     2761 ttgaaaagca caccgttgtc tctggcatac tgcaccaccg catctccaac agggtgattg
     2821 gcataccgtt caagcgttgc acataaagaa ataagttcat catgctgctc attgagggcc
     2881 acaacatcaa taacagtaag ctttcctgta gtaagagtgc ctgttttatc aaagacaacg
     2941 gcagtaacat tgcttgcctt ctccagtgct tcagcatttt taataagaat ccccaactca
     3001 gcacccctcc caagaccaac cgtcacagca gtgggagttg caagtcccag cgcgcatgga
     3061 caggctataa cgataactgc aacaaaaacc tgaaacgcaa aactggcatt gccggtaaac
     3121 acataccaca acaccgaagc tacaaaagca ataatcagta tgacaggaat aaaataccct
     3181 actactctgt cagcaaaccg ctgcattgct ggttttgact cttgagcctg ctgtaccagt
     3241 gaaataatcc gtgagagtac agaatcgctt cccagtacct gtgcctgtat tacaagaaga
     3301 ttgtttttat tcagtgttcc accaataacc ctgtcacctt cctttttata caccggcacc
     3361 gattcaccag ttaccattga ttcatcaatg tacccagcac cttttataat cacaccatca
     3421 accggtacac gcgactgtgg ttttacaatg atactgtctc ccacaacgac atcttcagtt
     3481 ttaatttcaa tttcattgtt atcacgtata acagtagccg tgtcgggttt aagcgatagt
     3541 aactttttaa tggtccctga tgttcttgac ctggctctat cctctaaata cttcccaata
     3601 ttaagaaatg ttgctaaaaa aatagcggtt tcatacacaa caaactcatg cggtaaaata
     3661 ccaaacgtag cacatacact tgctacaaac gatacacccg tgcccatggc atacataaca
     3721 tccatggata aaaccctgtt tttaagtgca tggtaggcat ggacaaaaat atgaaaactt
     3781 ataaacacaa atacaggcat agagagcaaa gcagccaccc agtgactgaa aatctccggg
     3841 acatacatca ttgccataag t
//
LOCUS       DAOSZP010000159         3832 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_19198_length_3832_cov_2.953402, whole genome shotgun sequence.
ACCESSION   DAOSZP010000159 DAOSZP010000000
VERSION     DAOSZP010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3832
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19198_length_3832_cov_2.953402"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="PK348_04595"
     CDS             <1..318
                     /locus_tag="PK348_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008727942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65524.1"
                     /translation="GKIKAITLGRQGTRVTVVKIEHDNGIIRMSGNDFRLMFDAKKIK
                     SLYFEAKQTRTGLILTGHGWGHGVGMCQWGAKEMAQQGMRFDQILKFYYKGIRIMTYS
                     MQK"
     gene            652..2010
                     /locus_tag="PK348_04600"
     CDS             652..2010
                     /locus_tag="PK348_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HXK65525.1"
                     /translation="MKKMSISVLFILFGICVLAIAQTQKFGEALHGLYAYKDFQKATY
                     CRQCHVEIYYQWQKSMMAQAFVHPWDEIEYFDLAVSHGRRNPKFKPVADGCNGCHAPI
                     SFMAGDTPPPRPEKKSMANEGVSCDVCHTIVSFNQNEQYNFSFFAKPGRTKYGPRGGK
                     NSPAHDIVKSDLITKPEFCANCHNEKSPWGVWVKATYNEWKEGPYSKEGVVCQTCHMP
                     KAAGKRAKTENKLYDDLKQHTFHGGHFQAKYNGAIDVLIYPDTDFVEPGMKTKISVYL
                     YNQKCGHKVPTGSVEDRLLYLHVEAIDSKGNVYHLKVDKKGFQGEEYTIASREKAYQD
                     FAEMMDVPADFDGIIREDVPVGDRIFRMPYFTENGIMTIAQWNTAKFGVDYRIAPRET
                     KVETYTWTVPDTVAPGPLTIRATLYYQLLVRPVAQFLKVPESESMDRMINTDVVTIDV
                     IY"
     gene            2042..3271
                     /locus_tag="PK348_04605"
     CDS             2042..3271
                     /locus_tag="PK348_04605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65526.1"
                     /translation="MKTCTMYTIIVLLVIGVLLYCEEQSQAMPGFARRYKFTCQTCHA
                     PFPKLKPYGEEFAANGFRLPEGEPSYATIDTGDPSLELTRNFPIGMRLDAYAVADKDD
                     EVSNDLQTPILLKFISGGPIAQNLSYYFYFFFSEKGDVVGIEDAFLYYTDLLGTGINI
                     TAGQFSVSDPIYKNELRLTYESYVPFAAKPNRSLCNLKYDRGIIVDKSFDFGLTLVAE
                     IVNGNGIDPADEHDNFDGDKYKNYFFRASQELFDVIRIGGFGYIGKEKNTADDTVTDV
                     FTNSVFYYGGDLSIILGPFELNTLFLYRKDSNAYFTENKKSIATKGYMAELIFQPEGD
                     RSNYVITALYNKVDSDYRESGVHSLNYESATLSLTYLLARNVKIIGEYTYLLHDYSKG
                     EDYKNHHILKTGVSAAF"
     gene            3326..>3832
                     /locus_tag="PK348_04610"
     CDS             3326..>3832
                     /locus_tag="PK348_04610"
                     /inference="COORDINATES: protein motif:HMM:NF014592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HXK65527.1"
                     /translation="MEKKLYLLESYDFDLPQELIAQYPADKREESRLLILDRKSKHLV
                     HEKFYNLPNYINANDILIFNNAKVIPARIYCKRSTGATIEVVLTKRLCDNTWEAICNR
                     LKRIKPGEILYPIKNNSVSIVVVEKSEEGITIKFEPNIDYAMLEKIGELPLPPYIQRS
                     PDKNDYERY"
BASE COUNT         1295 a          609 c          797 g         1131 t
ORIGIN      
        1 ggaaaaatta aggctataac cttaggacgc caaggtaccc gtgtaacagt ggttaagata
       61 gaacatgata acggcattat cagaatgagc ggaaatgatt tcaggttaat gtttgatgca
      121 aaaaagataa aaagcctgta ttttgaagca aaacaaacgc ggacaggatt aattttgacg
      181 gggcatggat ggggtcacgg tgtaggaatg tgtcagtggg gtgcaaaaga gatggcacaa
      241 cagggtatgc gttttgatca gattcttaag ttttattata aaggcatacg aatcatgaca
      301 tattctatgc aaaagtaaac atgttatctt tgctctgaat aatagaattg ccgggaacat
      361 tatcacacct ggataaggat tggtagccgc acttcgattg aattcagtaa ttgcttttca
      421 attaaagatt aaacttaatc aatcatgtta tgattgtatc atcttcaggt attccttcac
      481 cgtataatct caatgctttt tattaactac atttgcaacc attgataaac cctgcaatcc
      541 attacatgaa ctatcgcaca taccaaaaaa ttacagtgac aatagtatta aatataatct
      601 tgactttgat atttgttcat tgtaaaataa acatatgcca ggaggaagta tatgaaaaag
      661 atgagtatca gcgttctttt tatactcttt ggaatatgcg ttttggctat agcgcaaaca
      721 caaaaatttg gtgaagcatt acacggatta tatgcatata aagattttca aaaggcaaca
      781 tactgtcgtc aatgtcatgt ggaaatttat taccaatggc aaaagagtat gatggcacag
      841 gcttttgttc atccatggga tgagattgaa tattttgatt tagctgtttc tcatggcagg
      901 cgaaatccaa aatttaaacc tgttgctgat ggctgtaatg gatgccatgc gccgatttca
      961 tttatggcag gagatacacc tccgccacgg ccagagaaaa aatcaatggc aaatgaaggc
     1021 gtaagttgtg atgtatgtca taccattgtg tcgtttaatc agaatgaaca gtataacttc
     1081 agtttctttg caaagcctgg aagaacaaaa tatggaccaa gaggaggcaa aaattctcca
     1141 gcacatgata tcgtaaaatc agatttaatt accaagcctg aattttgtgc caattgccat
     1201 aatgaaaaaa gcccatgggg agtatgggtt aaagcaacat ataatgagtg gaaagagggc
     1261 ccatatagca aagagggggt agtgtgccag acatgccata tgcctaaagc ggcaggcaag
     1321 cgagcaaaga ctgaaaacaa attatacgat gatcttaaac agcatacatt ccatggaggc
     1381 cattttcagg ccaaatacaa tggtgcaata gatgttctga tatatcctga tacagatttt
     1441 gttgaaccag ggatgaaaac aaagatatca gtttatctgt ataatcaaaa gtgtggccat
     1501 aaagtgccaa ctggttcagt tgaagacagg cttttgtatt tgcatgttga agcaattgac
     1561 agcaaaggaa atgtatatca tctgaaagtt gataaaaaag gatttcaagg ggaagaatac
     1621 actattgctt cacgtgagaa ggcatatcag gattttgccg agatgatgga tgtaccagca
     1681 gattttgatg gcataatccg tgaagatgtt ccagttgggg atagaatatt cagaatgcct
     1741 tattttactg aaaatgggat tatgactatt gctcagtgga atacagcaaa gtttggtgtg
     1801 gattacagaa tagcgccacg ggaaacaaaa gttgaaacat atacatggac cgtccccgat
     1861 actgttgcac cggggccatt gacaatacgg gcaacattgt attatcaatt actggtaaga
     1921 ccagttgcac aatttttaaa agtgccggag tcagagtcaa tggatagaat gataaatact
     1981 gatgttgtaa caattgatgt tatatattaa ataaaagaca gccaacggga ggagcagtgc
     2041 aatgaaaaca tgtaccatgt ataccattat tgtattattg gttattggtg tactattata
     2101 ttgtgaagaa caatcacagg caatgccagg atttgcacgc aggtataagt ttacctgcca
     2161 gacatgccat gcgccttttc ccaaacttaa accttacggg gaagaatttg ctgcaaatgg
     2221 attcaggctg cctgaaggtg agccttcata tgcaacaata gataccggtg atccttcact
     2281 ggaattgaca cgtaatttcc ctattggcat gcgactggat gcgtatgcag ttgcagataa
     2341 agatgatgaa gtatctaatg atctacaaac accaatacta ttgaaattta tttcgggtgg
     2401 ccctatcgca cagaatttat catattactt ttactttttc ttttctgaaa aaggggatgt
     2461 tgttggtatt gaagatgctt tcttgtatta tactgattta ttgggaacag gtattaatat
     2521 aacggcaggt caattttcag tatctgaccc catctacaag aatgaactca gattgacgta
     2581 tgaaagctat gttccgtttg ctgcaaaacc caatcgttct ttatgcaatt taaaatatga
     2641 tcgtggaata attgttgata aaagctttga ctttggatta acgttagttg cggagatagt
     2701 aaatgggaat gggattgatc cggcagatga acatgataat tttgatggcg ataagtataa
     2761 gaattacttc tttagagcca gtcaggaact ttttgatgtg ataaggattg gtggttttgg
     2821 ttacataggt aaagagaaaa atacagcaga tgatactgtt accgatgtgt tcacgaatag
     2881 tgtgttttat tatggtgggg atttgtcaat tattttggga ccgtttgagc ttaacacatt
     2941 atttctgtac cgaaaagata gtaatgcata ttttactgaa aataaaaaat caattgccac
     3001 aaaaggttat atggcagaat tgatatttca gcccgaaggt gaccgcagca actatgtgat
     3061 tacagcacta tacaataagg tagattcaga ttatcgggaa agtggagtcc attcattaaa
     3121 ttacgaaagt gcaacactat cgttaacata cctcttggca cgaaatgtaa aaataatagg
     3181 tgagtatacc tacttattgc atgattattc aaaaggtgag gattataaaa accatcatat
     3241 attgaaaaca ggtgtttcgg ctgctttcta aaagataatt tatattttta ttgtgctata
     3301 attgctatca agataattgt caaacatgga aaagaaatta tatcttttag aaagctacga
     3361 ttttgacctc ccacaggagt tgattgctca gtacccggct gataaacgtg aagaatcacg
     3421 tctgcttatt cttgatagaa aatctaaaca cttggtgcat gaaaaatttt acaatttacc
     3481 taactacatt aatgccaatg atattctgat cttcaataat gctaaagtta taccagcccg
     3541 aatttattgt aaacgctcaa caggtgctac catcgaagtt gtcttaacaa aaagattgtg
     3601 cgataatacc tgggaagcta tctgcaaccg gttaaaaagg attaaacctg gtgaaatact
     3661 gtatcctatt aaaaataatt cagtgtcgat agttgttgtg gaaaaatcgg aagaaggtat
     3721 tacaataaaa tttgagccta acatagatta tgcaatgctt gaaaagatag gagagttgcc
     3781 tctaccaccc tatattcaac ggtcccctga taaaaatgat tatgaacgct ac
//
LOCUS       DAOSZP010000160         3822 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_19262_length_3822_cov_3.196443, whole genome shotgun sequence.
ACCESSION   DAOSZP010000160 DAOSZP010000000
VERSION     DAOSZP010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3822
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19262_length_3822_cov_3.196443"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            178..1185
                     /locus_tag="PK348_04615"
     CDS             178..1185
                     /locus_tag="PK348_04615"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HXK65528.1"
                     /translation="MFNPFKIFKNTKVGLALGSGGAKGVSHIAVLDYCTKKEIHVSMI
                     AGSSIGAVIGSLYAYGALPEFTSFLKSIKSNDLFAYFDPVFPKTGLVQGRRAMELLAK
                     FIPKSVDIEDLKLPVAIVATDFYSGKPVVFTKGNVLDAIRASISIPGIFVPVKYMDTV
                     LIDGGVSNPLPVDVVKQMGAGITIAVNLHPTIKIKRAIAPAIAKPHQESTNENSSNDI
                     IEHVDTESLDTTTVVTQTKGWLTQVKKWLGIKIESYKKIPEDTMPNIFEIVAGTIDIM
                     EYMNTTLMITYNKPDVLIEPDLVHVGTLDFTKVDFLLNEGYAACLRVEKDIKRKILRW
                     I"
     gene            1185..1892
                     /locus_tag="PK348_04620"
     CDS             1185..1892
                     /locus_tag="PK348_04620"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016546164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HXK65529.1"
                     /translation="MAQNKAQFLEDLRNNTIGNCTRCKLHATRSTIVFGEGNPEASVM
                     FIGEGPGKDEDAQGRPFVGKAGQLLTAVIEKGMKLKRSDVYIANVVKCRPTVNLELKR
                     DRPPDDEEVAACGPFLKKQIEIIQPKVIVTLGNPSTKFLVNPKEGITKVHGVWHLYNN
                     IPVMPTFHPSYILRNGGDSSPLKRLVWEDIKKVIAYLHGEITFEAISGKSEVTLDISI
                     NVAGDEKRDDEVQGSLF"
     gene            2035..3165
                     /locus_tag="PK348_04625"
     CDS             2035..3165
                     /locus_tag="PK348_04625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXK65530.1"
                     /translation="MASIEVKEIDITNKKMMKQFIMLPWTTKIYENDPAWVPPIIADQ
                     KKLFNPKTGYFYEIGEVAYFLAYKDGVPVGRITAHINRMYEEKYNKETGFFGFYEAID
                     DLDVAKALFAAAEGWLKKKGKTRVEGPQSFSIYDSVGFEVLGEDIMPVVGLFHFAPYY
                     KHHAEACGYTKCIDWHCFLVRKIDDFKPYLKDVRENIMKTQDVKYIYYSPKELKKRAK
                     EIHEIFNRAWDGNWGHLDLTQKQFDMLVDELKLVAVPELIVFAEKDGKTVGFIVSLPD
                     MNPALKILNGHLYPWRLIKALRSIKKVKKIRTIIMGVLPEYRGQKIDDVFYLYTIENG
                     IRLGYTESDCSLIVETNYKMIGALKPLKGQIYKTYRIYEKKI"
     gene            3162..3368
                     /locus_tag="PK348_04630"
     CDS             3162..3368
                     /locus_tag="PK348_04630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65531.1"
                     /translation="MIKNAKLLEEFEKECIIKHPLSYDEVLALLDGMWELGMHLGVLP
                     PEDPLEGIDVDIKVARILNCLKKK"
     gene            3350..>3822
                     /locus_tag="PK348_04635"
     CDS             3350..>3822
                     /locus_tag="PK348_04635"
                     /inference="COORDINATES: protein motif:HMM:NF021487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HXK65532.1"
                     /translation="MFKEEIKRICKALNKNKIPYMIVGGQAVLLYGEPRLTRDIDITL
                     GIDIDRLDDVLNIVKKINLKILTPDVKNFVKETMVLPVLGSRGIRIDMIFSFTEYERN
                     AIQRAKKILTDDVEISYASPEDVIIQKIFAGRERDIEDAKIIIAKQTVDDAYIMH"
BASE COUNT         1255 a          577 c          852 g         1138 t
ORIGIN      
        1 tatcgaatat ggagaattgg atgagctgtt tttggaaatt attccccagc ctcaattatt
       61 gcttacaatt tattatatga ataatacaat atattccctg ctttgggctg caggggtatc
      121 aattatatat tttatatgcg cacatttaag aataaatgat ggaatttaaa aaaaattatg
      181 tttaatccat ttaaaatatt taaaaacaca aaagtaggcc ttgcacttgg tagcggtggt
      241 gctaaaggtg tgtcacatat tgctgtgctt gattattgta ccaaaaaaga aattcatgtt
      301 tcaatgattg caggatcaag cattggtgct gtgattggtt cgctgtatgc atatggtgca
      361 ttgcccgaat tcacttcgtt tttaaaatca ataaaaagca atgatctctt tgcatatttt
      421 gatccagtgt ttccaaaaac aggactggtg cagggccggc gtgcaatgga gttacttgca
      481 aaatttattc ctaaaagtgt tgatattgaa gatttaaaac tgcctgttgc cattgttgca
      541 accgactttt atagcggaaa gcctgtggtt tttacaaaag ggaatgtcct tgatgccatt
      601 agggcaagta tttcaatacc aggaattttt gttccggtta aatatatgga tacggtgctt
      661 atagatggtg gtgtaagtaa tccgttacca gttgatgtgg ttaagcagat gggtgctggc
      721 attaccatag cagttaatct gcatccaaca attaagatta agcgggcgat agctcctgca
      781 attgcaaaac cgcaccaaga gtctaccaat gaaaattcat caaatgatat tattgagcac
      841 gttgatacag aatcacttga tacaacaaca gtagttactc agacaaaggg ttggctgacg
      901 caggttaaaa agtggcttgg cataaagatt gaaagttata aaaagatacc tgaagatact
      961 atgcccaata tttttgagat agtagccggg acaattgaca tcatggaata catgaacaca
     1021 acgctcatga ttacatataa taagcctgat gtactcattg aacctgacct tgtccatgta
     1081 gggacactgg attttacaaa agttgatttt cttttaaatg aaggttatgc agcctgtcta
     1141 agagttgaga aggatataaa acgcaagata ttgcggtgga tatgatggca caaaacaagg
     1201 cacagttctt agaggattta cgcaataata ctattgggaa ttgcacacgc tgtaaacttc
     1261 acgcaacgcg cagtaccatt gtttttggtg aaggtaatcc tgaagccagt gttatgttca
     1321 ttggcgaagg tccaggcaag gatgaagacg cccagggcag accatttgtg gggaaagcgg
     1381 gacagctttt gaccgctgtc attgaaaaag ggatgaagct taagcgcagc gatgtgtata
     1441 ttgccaatgt agttaaatgc aggccaacgg tcaacttaga gttaaagcgt gaccgtccac
     1501 ccgatgatga agaagtagca gcctgcgggc cgtttttaaa aaagcaaatt gaaattattc
     1561 agccaaaagt aatcgttacg ttagggaacc cttctacaaa atttttggtt aatccaaagg
     1621 aaggcattac aaaagtgcac ggtgtgtggc acctgtataa caatatccct gttatgccaa
     1681 cgtttcatcc ttcgtatatt ttacgaaatg gtggtgatag cagcccactc aaacgacttg
     1741 tgtgggaaga catcaaaaag gttatcgcat atttacatgg tgaaatcacg tttgaagcta
     1801 tttctggaaa atcagaggta accttagata tcagtattaa cgttgctggt gatgagaaac
     1861 gtgatgatga ggttcagggt agtttgtttt aacatgtttg tatatagtat caggtatgga
     1921 tagcggtaac tatcgcccat tcaatgtttt tttaattgat gctaccaggt tacggtttca
     1981 tcaattagtt tatatatact ctttagaatt ttaaactttt gtgggggtac atttatggca
     2041 tcaatagagg taaaagaaat tgacattaca aacaaaaaga tgatgaagca gttcatcatg
     2101 cttccgtgga ctacaaagat ctatgaaaat gacccggcgt gggtgccacc catcattgct
     2161 gatcaaaaaa agctctttaa tccaaagact ggttactttt acgaaattgg tgaagttgca
     2221 tattttttag cgtataagga tggtgtgcct gttggcagaa tcaccgctca catcaacagg
     2281 atgtatgaag aaaaatacaa taaagaaaca ggtttttttg gattttatga agccattgat
     2341 gacctggatg ttgcaaaggc tttgtttgct gctgctgaag ggtggttaaa gaaaaaagga
     2401 aaaacacgtg ttgaaggtcc tcaaagtttt tccatttatg actcggttgg ctttgaagtg
     2461 ctgggtgagg atatcatgcc tgttgtgggg ctattccatt ttgctccgta ttacaaacat
     2521 catgcagaag cgtgtggcta taccaaatgt attgactggc attgctttct ggtaagaaag
     2581 attgatgatt tcaagccgta tttaaaagat gttcgtgaaa acatcatgaa gacgcaggat
     2641 gtaaaatata tatactacag ccctaaagaa ttgaaaaagc gtgctaaaga gattcatgaa
     2701 atatttaata gagcatggga tggtaactgg gggcatcttg atttaacgca gaagcagttt
     2761 gatatgcttg ttgatgagtt gaagcttgtt gctgtgccag aacttattgt gtttgcagaa
     2821 aaggatggca aaacagttgg gtttattgta tccctacctg atatgaatcc agcactcaag
     2881 attttaaatg gtcaccttta tccatggcgt ttgattaaag cgttgcgttc aatcaaaaaa
     2941 gttaaaaaga ttcgtactat catcatgggt gtgttgcctg aatatagggg gcagaaaatt
     3001 gatgatgtat tctatctgta taccattgaa aatggcatac ggctgggtta taccgaatcg
     3061 gactgttctc tgatagttga aactaattac aaaatgatag gtgccttaaa accgcttaaa
     3121 ggacaaattt acaagacgta tcgaatttat gaaaagaaga tatgattaag aatgcaaaac
     3181 tgttagagga atttgaaaag gaatgtataa taaagcaccc cttaagctat gatgaggtac
     3241 ttgcattact tgatggtatg tgggagctgg gcatgcattt aggggtgctg ccacctgaag
     3301 acccgttaga ggggattgat gttgatatta aagtagcaag gatactgaat tgtttaaaga
     3361 agaaataaaa agaatttgca aagcattaaa taaaaataaa attccataca tgatagtagg
     3421 tggccaggct gtattgctgt atggtgagcc aaggcttacc agggatattg atattacatt
     3481 aggtattgac atagatagat tggatgatgt cttgaatatt gtaaaaaaaa taaatttaaa
     3541 aatattgacg cctgatgtta aaaattttgt gaaagaaact atggtattac ctgtacttgg
     3601 atcaagaggc ataagaattg atatgatatt ttcatttacc gaatatgaaa gaaatgcaat
     3661 acaaagagca aagaaaatat tgacagacga tgtagaaatt agttatgcat cccctgaaga
     3721 tgtaataatt caaaaaatat ttgctggaag agaaagggat attgaggatg caaaaattat
     3781 aattgcaaag caaacggtag atgatgccta tattatgcat tg
//
LOCUS       DAOSZP010000161         3807 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_19370_length_3807_cov_2.895789, whole genome shotgun sequence.
ACCESSION   DAOSZP010000161 DAOSZP010000000
VERSION     DAOSZP010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3807
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19370_length_3807_cov_2.895789"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            19..>3807
                     /gene="rpoC"
                     /locus_tag="PK348_04640"
     CDS             19..>3807
                     /gene="rpoC"
                     /locus_tag="PK348_04640"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004753095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HXK65533.1"
                     /translation="MRDFNDFNSIQIKLASPDMIREWSYGEVKKPETINYRTLKPERD
                     GLFCERIFGTTKEWECYCGKFKSIRYKGVVCDRCGVEVTHYKVRRERGGHIELACPVA
                     HIWYYRSVPSRIGLLLNLTVNELKSILYYEKYVVIDPAESDLKYGDVIDEDTFNHYYE
                     TYGDEFVADMGAAAIKEMLRRIDLDGEARKLRSKIAGQDKLKVDKKDIKRLELIEAFR
                     DSGNRPEWMILDVVPVIPPELRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLLLLR
                     APDIIVRNEKRMLQEAVDALFDNSKRKRAVKGKANRPLKSLSDMLKGKQGRFRQNLLG
                     KRVDYSGRSVIVIGPELKLHQCGLPKKMAVELFKPFIMKRLVDKGFVQNIKSAKKMVE
                     NERPEVWEVLEEVIAEHPVLLNRAPTLHRLGIQAFEPVLVEGKAIKLHPLVCHAYNAD
                     FDGDQMAVHVPLSPRAQIECWTLMLSANNLLNPANGQPIVTPTQDIVLGIYYLTSELK
                     SESENIPYFGSMDEVLYALEFRQIKYRTKIKYLMDGEFIETTAGRLIFNEKLPAGYPF
                     VNYTVNDKELSKIISKIFREFGPTATVKVLDDIKELGYKYATYFGPTISTSDVKVPPL
                     KDELIKKSNEMLEKIENEYRNGYITNEERYNRVIDLWTKTNEDVTEVMFNTMAQDAEG
                     FNPIYIMAQSGARGSKQQIRQLAGMRGLMAKPSGEIIDVPIKSNFKEGLTVLEYFIST
                     NGARKGLADTALKTADAGYLTRRLVDIAQDVVITMDDCGTSVGIDVGALKEGDEVVES
                     IGSRVLGRTLLYDLYHPVTNEVICKSGELITEEIAEKIDELGLDRITIRSVLTCEARH
                     GVCARCYGRNLATARMVDIGEAVGIIAAQSIGQPGTQLTMRTFHIGGTASHVVEESEI
                     SFNYPVYVKETTFKTVKTEDEQGEKKTISVRRGYIVLQRLLEEIPVKSGELVVNDGDR
                     VYSGTPIVKKSDGSFIVTTKSGVVKLDKSRAYILGEPNMIPVNIGSTIYLKTDKFYHV
                     NPQTGETDVLVKFDPWFEPILTEINGKVKFIDIEINKSMREEIDPQTNQLKRIIVEAK
                     TEKLQPAIEVSSADETFNYLLPKGAHLFVDDGQTVKAGMVLAKIPKEVEKTKDITGGL
                     PRVAELFEARTPKDKATISEIDGRVKIGDTVKNKRKIIVTGDDGEEREYQIPASKYLR
                     VQDGDWINKGDKIDDGPIDPHDIIDIKGPRELEKFLVNEIQEVYRLQGVQINDKHIEI
                     I"
BASE COUNT         1242 a          622 c          907 g         1036 t
ORIGIN      
        1 aggtaagaag gtaatacgat gagagatttt aacgatttca attctatcca aataaaatta
       61 gcttctcctg atatgatccg tgagtggtca tatggtgaag tgaaaaaacc tgaaacaatt
      121 aactatcgca ccttaaaacc tgaaagggat ggcctttttt gtgaacgaat atttgggaca
      181 acaaaagaat gggaatgcta ttgcggcaag ttcaagtcta tacgatataa aggtgtagta
      241 tgcgaccgtt gtggcgtgga ggttacacat tataaggtca ggcgtgagcg tggtggtcat
      301 attgaattag catgcccggt agcgcatata tggtattatc gctcagtacc atcacggata
      361 gggttgttgc ttaatttgac agtaaatgag ttaaaatcta ttctgtatta cgaaaaatat
      421 gttgttattg accctgctga gtctgattta aaatatggtg atgtgataga tgaagatacg
      481 ttcaaccatt attatgaaac atacggcgat gaatttgtag ccgatatggg tgcagctgca
      541 atcaaggaaa tgttgagaag aatagacctt gatggcgaag cacgtaaatt gcgaagcaaa
      601 attgcaggtc aggataaact gaaggtagat aaaaaagata ttaaacgact tgagcttata
      661 gaagctttca gggattcagg caaccggcct gagtggatga tacttgatgt tgtaccggtt
      721 attcctccgg aactgaggcc aatggttcag cttgatggtg gaaggtttgc aacatctgat
      781 cttaatgacc tttacaggcg agttattaac agaaataacc ggttaaaacg attgctgctt
      841 ctgagagcac ctgatattat agtaagaaat gaaaaacgta tgctccagga agcggtggat
      901 gcactgtttg ataacagcaa gcgcaaacgc gcagtgaaag gcaaagccaa caggcctctt
      961 aagtcactca gtgacatgtt aaaaggcaaa caggggcggt ttagacagaa ccttctgggt
     1021 aagcgagttg attattccgg gcgttcagtt attgttattg gccctgaact taaactgcat
     1081 cagtgtggat tgccaaagaa gatggctgtg gaattattta agccatttat catgaagaga
     1141 ctggtagata aaggttttgt acagaatata aaatctgcca aaaagatggt ggaaaatgaa
     1201 aggcctgaag tgtgggaagt tttagaagag gttattgccg aacatccggt actgttaaac
     1261 cgtgctccca cactacaccg gttgggaatt caggcgtttg aaccagtact cgtagagggg
     1321 aaggctatca agctgcatcc attggtatgc catgcataca atgctgactt tgatggcgac
     1381 cagatggctg ttcatgtccc actatcgcct cgagcccaga tagagtgctg gacattaatg
     1441 ctttcagcaa acaatttgct taaccccgca aatggtcaac ctatcgtaac tccaacccag
     1501 gatattgttc ttgggatata ttatttaacc tctgaattaa agagtgaaag tgaaaatatt
     1561 ccgtattttg gttcaatgga tgaggtgctc tatgctcttg aattcagaca gataaagtac
     1621 agaacaaaga ttaaatatct gatggatggt gaatttattg agacaacagc aggcaggtta
     1681 atctttaacg agaaacttcc agcaggctac ccttttgtta actatactgt caatgataag
     1741 gaactttcaa aaattatttc taaaatcttc agagagtttg ggccaactgc aacagtaaag
     1801 gtgcttgatg acatcaagga attgggctat aagtatgcaa catattttgg gccaactatc
     1861 tcaacaagtg atgtaaaagt gcctccattg aaggatgagt tgataaaaaa atctaatgag
     1921 atgctggaaa agattgagaa tgaataccgt aacggttata taaccaatga ggagcggtat
     1981 aatcgtgtta ttgatttatg gacaaaaacc aatgaagatg ttactgaggt aatgttcaat
     2041 accatggctc aggatgctga aggatttaat cccatttata ttatggcaca gtccggagca
     2101 cgaggcagta aacagcagat acggcagctt gcaggtatgc gtggtcttat ggcaaaaccc
     2161 agcggtgaaa ttattgatgt ccccatcaag tcaaatttta aagaagggct gacagtactt
     2221 gaatatttta tctccactaa cggtgcacga aaaggcttgg cagatactgc attaaaaact
     2281 gctgatgccg ggtatttaac acgaaggctt gttgatatag cccaggatgt tgttataaca
     2341 atggatgatt gcggtacctc agtaggaatt gatgtgggtg cattaaaaga aggtgatgaa
     2401 gttgtggaat ctattggttc aagggtactg gggagaacct tgctttatga tctgtatcat
     2461 ccggttacca atgaagttat ttgcaagagt ggtgaattaa ttactgaaga gattgcagaa
     2521 aagatagatg aattgggcct tgatcgtata acaatcagat cggtgttaac gtgtgaagct
     2581 cgacatggtg tttgtgcacg ctgttatggc agaaatttag ccactgcacg tatggttgat
     2641 attggtgaag ctgtagggat tattgcagca caatctattg gtcagccagg tacacagctt
     2701 accatgcgta ccttccacat tggtggtact gcatctcacg ttgttgaaga aagtgaaatc
     2761 agttttaatt accctgtcta tgtaaaggaa actaccttca aaacagtaaa aactgaagat
     2821 gaacagggtg agaagaaaac catatctgta cggcgtggtt atatcgtttt acagcgatta
     2881 ctggaagaga tacctgtaaa aagtggtgaa ctggtggtaa atgatggtga cagagtatat
     2941 agcggaacac caatagtaaa aaaatctgat gggtcattta ttgtcacaac aaaatcaggt
     3001 gtggttaaac ttgataaatc cagggcatat atccttggag agcccaatat gattcctgtt
     3061 aatataggtt ctaccattta tttaaaaact gataaatttt atcatgtaaa tccacagacg
     3121 ggggaaactg atgtactggt gaaatttgat ccctggtttg agccaatatt aactgaaata
     3181 aatgggaagg tgaaattcat tgatattgaa atcaataaga gtatgaggga agagatagat
     3241 cctcagacaa atcagctcaa gcgtattatc gttgaagcaa agacggaaaa gttgcagcca
     3301 gccatagaag tttcatctgc tgatgaaacc ttcaactacc tgttgccaaa aggtgctcac
     3361 ctgtttgttg atgatggaca gacagtaaaa gcaggtatgg ttctggcaaa aataccaaaa
     3421 gaagttgaaa agacaaaaga tattacgggt ggcttgccac gagtagcaga actctttgaa
     3481 gcacgtaccc ccaaggataa ggcaaccata tcagagattg acgggcgtgt caaaattggc
     3541 gataccgtca agaataaacg taagataatt gtcactggtg atgatggcga agaaagagaa
     3601 tatcagatac ctgcttccaa gtatttacgg gttcaggatg gcgactggat caataaaggt
     3661 gataaaattg atgatggccc aattgatccg catgatatca ttgatattaa aggaccgcgc
     3721 gaacttgaaa aattccttgt aaacgaaatt caggaagtgt atcgtttgca aggtgttcag
     3781 ataaatgata agcacattga gataatt
//
LOCUS       DAOSZP010000162         3802 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_19403_length_3802_cov_2.451294, whole genome shotgun sequence.
ACCESSION   DAOSZP010000162 DAOSZP010000000
VERSION     DAOSZP010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3802
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19403_length_3802_cov_2.451294"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(397..1848)
                     /gene="putP"
                     /locus_tag="PK348_04645"
     CDS             complement(397..1848)
                     /gene="putP"
                     /locus_tag="PK348_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013278487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015824 - proline transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HXK65534.1"
                     /translation="MITTFITFGLYLVLLLAIGVYYYNKSHDISDFILGGRKLGKFVM
                     ALSAQASDMSGWLLMGLPGALYTKGMPAIWIAAGLLLGTYCNWKFVAKRLRIYTEITD
                     SITLPTFFEDRFKDPTGLLRIISALIILIFFTIYLSSGFVASGKLFESMLGIEYHIAV
                     LIGAGIILFYTSLGGFFAVSVTDVVQGSLMFIAIIVVPIVSMQHIGSFNTITHAMQKA
                     SLPINPFTSITLLSVVSTASWGLGYFGQPHILARFMGIHSHHDIKSARQIAMTWVTIS
                     LCGASFIGIAAVYLFPELSQHNAEKVFIFMIQKFFTPWIGGILLAAILSAIMSTADSQ
                     LLVCSAALTEDFYQRIIKRNASQKELIHIGRITTMLITIIAALFAMNPENTVLGLVAY
                     AWGGFGAAFGPIVLFALFSKNTSWQSVLGGMLAGTIALVSWNFLGLTNYCYEIVPGFI
                     ANVITIMVLNRIYPPQNSTINTEFATMKKEIQS"
     gene            2165..2494
                     /locus_tag="PK348_04650"
     CDS             2165..2494
                     /locus_tag="PK348_04650"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HXK65535.1"
                     /translation="MIELTDKGNGTFVVKIIMQEVHTLDVPDLKEKLQQAIVNKGIKR
                     MVVDLSDVKMITSSGIGIFLNINQNLKSQFRLAAPTPEVQKVLELTKVTSMIKVFNSV
                     EAALQSF"
     regulatory      2747..2850
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            2925..>3802
                     /locus_tag="PK348_04655"
     CDS             2925..>3802
                     /locus_tag="PK348_04655"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013825522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HXK65536.1"
                     /translation="MKHDITLDTSVGIVQTQYYTFAEPPDEMVLASGKKLGPITLAYE
                     TYGTLNESKSNAILILHALSGSAHAAGYHTEADAYPGWWDHYIGPDKAFDTSKYFVIC
                     SNVIGGCSGSTGPSSVNPHTGKEYALDFPIVTIQDMVNAQRHLLDYLGIEKLLAVAGG
                     SMGGMQALQWSISYPDRVQSVIAIATAASLSAQGIAFHEVGRQAIMRDPNWNNGDYYH
                     ATSPSNGLSLARMIGHITYLSEKLMHEKFGRRLQQADIFKFQFDLEFEVESYLHHKGN
                     DFVQRFDANSYLYLTKA"
BASE COUNT         1265 a          646 c          739 g         1152 t
ORIGIN      
        1 ataattaaga aatgtgaggt attcacagat tgtagcacct aaattaaatg gtttattgga
       61 agcacctatt acagcaattg accgtgggtt aaaaaaagca tccatagata gctccattat
      121 aatgtagtat ttgtatgaca acttacaaaa tatggtattt ttatcaataa aaaaaatata
      181 gttattttat attatttttt ataattttgt gacaaagggg aattattata tacggtatat
      241 attgcctaaa tgtttcagaa aaaaaatgat agcaattatc ttcatcgtta taagcaaaaa
      301 agatgaataa tgtattattg tatattaaaa agcattttta tgagtgatta tttcaaatat
      361 aatacccttt tatattttta atacgtgtat tgattgttat gattgtatct cttttttcat
      421 tgttgcaaat tcggtattga ttgtggaatt ttgtggagga tagattctgt taagtaccat
      481 aatggttatt acatttgcaa taaatccagg aactatctca taacaataat tagttaatcc
      541 taaaaaattc catgaaacca gcgcaattgt gcctgcaagc attcccccca gtacggattg
      601 ccatgatgta ttctttgaaa aaagtgcaaa aagcactata ggaccgaatg cagcaccaaa
      661 accaccccat gcataggcta ccagtccaag aactgtattt tcaggattca tggcaaagag
      721 tgccgcgatg attgtgataa gcattgtggt aattcgacca atgtgaatta attctttctg
      781 tgatgcgttt cgtttaatga tacgctggta aaagtcttca gttaatgctg ctgaacacac
      841 caacaattgt gaatcggctg tggacatgat tgctgaaagt attgctgcaa ggagtattcc
      901 tccgatccag ggtgtaaaaa atttctgtat catgaaaata aaaacttttt ctgcattgtg
      961 ttgcgatagt tccgggaata gatacactgc tgcaatccca atgaaggaag caccacataa
     1021 tgaaatggtt acccatgtca ttgcgatctg acgggcactc ttgatatcat ggtgtgaatg
     1081 tattcccatg aaacgtgcca gtatatgcgg ttgaccaaaa tagccaagcc cccatgatgc
     1141 agtagacact actgagagta acgttattga tgtaaatggg ttaattggca gagatgcctt
     1201 ttgcatggca tgagttatag tgttaaaaga tccaatgtgc tgcatggaaa caatggggac
     1261 aacaataatg gcaataaaca taagtgagcc ctgaacaacg tcggtaacac tgacagcaaa
     1321 gaaaccacca agggatgtgt aaaaaagaat gatgcctgca ccaatcagta ctgcaatatg
     1381 atattcgatg cccagcatgg actcaaatag ttttcctgat gcaacaaatc cagaagaaag
     1441 ataaatagta aaaaatataa gaataattaa cgctgatatt atacgaagaa gccctgtggg
     1501 atctttgaag cgatcttcga aaaatgttgg cagtgtaata gaatctgtaa tttcggtata
     1561 aatacgaagc cgttttgcca caaatttcca gttacagtat gttccaagca aaagtccagc
     1621 tgcaatccat atagcaggca tgcctttagt atacaatgca cctggcaatc ccatgagtag
     1681 ccaaccactc atgtcactgg cctgtgctga taatgccatg acaaatttgc ctaatttcct
     1741 accacccaga ataaaatcag agatatcatg tgatttatta taataatata ctccaattgc
     1801 cagtaataga accagatata atccaaaggt tatgaaggtt gttatcatag cctgctatcc
     1861 tgtattaatt aaaaaactta tcaatgattg caatgtatat tcttgattgt caatgtaata
     1921 tcagtattta tttgttaact tttttaagga aagaaatatt ttaccaccca cgctatcgaa
     1981 ggcatcagtg gtatatattt catagtagtg ctctaacaag catttaaggt agtattactg
     2041 atgcaatgga taaaaaataa tatataaatt aacaacataa gaaatcaatt tttttattga
     2101 ctaaaaaaaa cactgtgact attatttcaa atgattgagc acattacaga aaaaggtgag
     2161 cggtatgata gaattaacag ataaaggaaa tggtacattc gttgtaaaga taatcatgca
     2221 ggaagttcat acattagatg ttccggatct gaaagaaaag cttcaacagg ctatagtcaa
     2281 taaaggaata aagcggatgg ttgttgacct ttcagatgtt aagatgatta ccagctctgg
     2341 tataggtatt tttctaaaca tcaatcagaa tcttaaatcg caattcagat tggctgcacc
     2401 tactcctgaa gtacagaagg tgttagaatt aacaaaagtt acatcaatga taaaagtatt
     2461 taattcagtt gaagcagctc ttcagagttt ttgatgtaaa tggaataata tactctcgcc
     2521 actgaaagca gcgtgagtgt atacattcat aatcaattat aaaaaacatg aatactgttt
     2581 tcctttttat tactaatttt gaaaatatat tttgatttca tgccgtatta aaatttattt
     2641 tctcattatc ggtaatgtgc tatatttgtg ctaaattagt atggtgatat attcgatata
     2701 gtattttgag tttttattat agcacgatta taattttaat cgtactatct tatcaagagt
     2761 ggtggaggga ctggccctgt gaagccacgg caaccggcac aggtaaaagt cggtgccaat
     2821 tccagcggaa gaaattccgg aagatgagat aggcaactgg taaccgctta ttctaaactt
     2881 ccggtaagcg gttttttatt tttatatagg agatctactg ttaaatgaaa catgacatta
     2941 cattagatac atccgttggc atagttcaaa cgcagtatta tacatttgca gaaccaccgg
     3001 atgaaatggt gcttgcctca ggtaaaaaac tggggcccat aacgttagcg tatgaaacat
     3061 atggaacatt gaatgagagc aaaagtaatg caatattaat tctgcatgcc ctttcaggat
     3121 ctgcacatgc tgcaggctat cacacagaag ctgatgcgta tcccgggtgg tgggatcact
     3181 atattggtcc agacaaagca tttgatacca gcaaatattt tgtcatctgt tccaatgtta
     3241 ttggtggttg cagtggttct accggcccct catcagtaaa tccacacacg ggtaaagaat
     3301 atgccctgga ttttcccatc gtaaccatac aggatatggt aaatgctcag agacatttac
     3361 ttgattatct gggaattgaa aagcttctgg cagttgccgg tggctcaatg ggtggcatgc
     3421 aggctttgca gtggtcaata tcatatcctg acagggtgca atcggttata gcgatagcaa
     3481 ccgcagcgtc tctctcggca cagggtattg ccttccatga agttggcagg caggctatca
     3541 tgcgtgaccc aaactggaat aatggggatt actatcatgc aaccagtcca tccaatggac
     3601 tatcgcttgc ccgaatgatt gggcatatca catatctcag tgaaaagctc atgcatgaaa
     3661 aatttggacg aagattacag caagccgata tatttaaatt tcagtttgat ctggagtttg
     3721 aggttgagtc atacctgcac cataagggaa atgattttgt ccagcgattt gatgcaaatt
     3781 cgtatctgta ccttacaaaa gc
//
LOCUS       DAOSZP010000163         3788 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_19480_length_3788_cov_4.300027, whole genome shotgun sequence.
ACCESSION   DAOSZP010000163 DAOSZP010000000
VERSION     DAOSZP010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3788)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3788)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3788
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19480_length_3788_cov_4.300027"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(450..2786)
                     /locus_tag="PK348_04660"
     CDS             complement(450..2786)
                     /locus_tag="PK348_04660"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HXK65537.1"
                     /translation="MKKIIIILVVVIVILGAVIYLFLRTGLPDYTSSLQVPGLASPVT
                     IERNKFAVPTITAQNMDDLYFAWGYVNAQDRLFQMEFTKRVGQGRISEFAGEEALSKD
                     YFLRAVGFYERAKEYTKKLSPYYKRLCQRYADGVNYFIETKGRNAYMILLGMKKEKWE
                     IADSVLVGMMLNWSLAYNMKYELLYHKIVQKIGKEKASLLLNFVPPDTPTVIGDSSGK
                     WLDDAHVSSLLKEFDWLFGCRSASNNWAVAPQLTSHGKAILCSDMQVHQSKLPNDFYL
                     IHVKAGDFEVAGAQVTGLPFIASGFNKHCAWGLTNQGADMVDLFKERIDWKAKRYHYN
                     GKWLPLKNRKEVFAVKGKQPIVKTMYYAGKKPVLTEVFKDLNFDVSLDWTGFDAIALE
                     GFFMINAAKNFDEFMEGAKQIRMSPQNLVYADDKGNIAFRLIGSLPVRKKGTGNFIQD
                     GEKIYSNWNGNIPDDKYPILKNPERGYIATANNLNAADYPYELNGTYAPGYRYENIAT
                     MLRNKKDLDVNYMKKMQTDTHTVLAKKVKAILKKFIQRDALDAYMQKAFDRVIGWDAD
                     SSKDSIGASVYNTFYVRFAYQTLVDELGDTVTEEYVSERYISMERFFEMVEKGSVFFD
                     DVRTNKSETVADIAQRAFKETCQLLCDYFGTDNVEKWQWGKIHSIKFDHILGMSALLK
                     PLVNYGPFPFEGDGETNNRAKFLEVAPPFIADLASAPRIIVKFTPQPEASMMLITGEN
                     EHFLSKHNTDMVDAWLDHVYFELGKGEVAYSMELIPEK"
     gene            complement(2860..3600)
                     /locus_tag="PK348_04665"
     CDS             complement(2860..3600)
                     /locus_tag="PK348_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015878491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HXK65538.1"
                     /translation="MVIRNIQAKSILSVSKVYDYVVNPYVGCQFGCLYCYARYMKKFT
                     GHKEPWGTFVDSKDNAAVLLQKEIAKKKKGLVWISGVCDPYQPLEKKYAITRACLTIL
                     IENDWPVIIQTKSNLVLRDVDIIKNASSCDVGLSITTANDSIRKLFEPQSTAIADRID
                     AIKQLKKHGIRTYVMIAPMLPGCENLIDMLAGSVDYVYVDKMNYHYADSVYKKYALEQ
                     YCDNSYFFSASHEIAYRCKKEGIACSIV"
BASE COUNT         1157 a          860 c          569 g         1202 t
ORIGIN      
        1 cgttgtattg tccatataca acctgcatat cgatattcat attgtgcaag ctctgaacat
       61 cattccgagc accatcctga gcaaaaataa aagaaccaaa caccacaccc tgtagtataa
      121 gtgccatgaa tatataaaga caccttttgc tattatgtat attttccatg caaccccata
      181 ctgtatatgt aattcattaa ggatgaatac tatcgtaaaa atggaatgta atgaaggttg
      241 attattgaaa cagggatgtt ttaagtcaat acaaatatga acaagttttc atacaaacac
      301 ctgtttcatc aataattata ttgtaataac catgcatttc ccattgtaat gcttcattag
      361 ctcaattgtg gtgagcatca ttatctctta acacaataac tgccaataag ctattgtgct
      421 gtcacaagca ctatatgcac ttcaatgcgt tatttctcag ggatcaattc catagaataa
      481 gccacctctc ccttacccag ttcaaaatat acatgatcaa gccatgcatc aaccatatca
      541 gtattatgct tacttaaaaa gtgttcattt tcccctgtaa tgagcatcat ggatgcttca
      601 ggttgtggag taaactttac aataatacgc ggagctgaag ctagatcagc aataaacggg
      661 ggagcaacct caagaaattt tgcacggtta tttgtttcgc catcaccttc aaaagggaac
      721 ggaccataat tgacaagggg ctttaaaagt gctgacatcc caagtatatg atcaaatttt
      781 atgctatgaa tttttcccca ttgccatttt tcaacattat ctgtaccaaa ataatcacac
      841 agcaactgac atgtttcttt aaatgcccgt tgtgctatat ctgcaacggt ttcacttttg
      901 tttgtccgca catcatcaaa aaaaacactc cccttttcaa ccatctcaaa aaagcgttcc
      961 atgctgatat atcgctcact tacatactcc tctgtaactg tatcacccaa ctcatcaacc
     1021 agagtttgat acgcaaatcg cacataaaat gtgttgtaaa cagaggcgcc aatagaatct
     1081 ttagaactat cagcatccca tccaattacc ctatcaaatg ccttttgcat gtaagcatca
     1141 agtgcatcac gttgtataaa ctttttcagt attgctttaa ccttttttgc aagcactgta
     1201 tgagtatcgg tctgcatttt tttcatataa ttaacatcaa gatccttctt atttcgcagc
     1261 atcgtcgcaa tattttcata gcgatagccg ggcgcataag taccgttcaa ctcataagga
     1321 tagtcagcag cattgagatt atttgcagtg gcaatgtacc ctcgttcagg atttttcaat
     1381 atgggatact tatcatcagg tatatttcca ttccagttgc tgtatatctt ttctccatcc
     1441 tggataaaat tgccagtacc cttttttcgc accggcagtg agccaatgag cctgaatgca
     1501 atatttccct tatcatcagc ataaaccaga ttctgaggcg acatcctgat ctgttttgcg
     1561 ccttccataa attcatcaaa atttttagct gcatttatca taaaaaaacc ctctaatgca
     1621 attgcatcaa aaccggtcca atcaaggctt acatcaaaat tcagatcttt aaatacttca
     1681 gttaacacag gtttcttccc tgcataatac atggtcttta caattggttg tttccctttt
     1741 acagcaaata cctcttttct attttttaat ggcagccatt ttccattgta atgataacgt
     1801 tttgctttcc aatcaatcct ttctttaaaa aggtcaacca tatcggcacc ctgattggta
     1861 agcccccatg cacaatgctt gttaaatcct gaagcaataa acggcaagcc cgttacctga
     1921 gcacccgcaa cttcaaaatc gccagcttta acatggataa gataaaaatc gttgggaagt
     1981 tttgactggt gaacctgcat atcgctgcac agtattgctt tcccgtgcga tgtcagttgc
     2041 ggagctaccg cccagttatt ggaagcagag cggcagccaa aaagccaatc aaactctttc
     2101 agcaaagagc taacatgtgc atcatccagc cactttccgg aactatcgcc aataaccgtt
     2161 ggcgtatcag gtggtacaaa attcaaaagt aacgaggctt tctctttccc tattttctga
     2221 acaattttgt ggtacaacag ttcatacttc atattatatg ccagactcca gttaagcatc
     2281 atgccaacca gcactgagtc tgctatctcc catttttctt ttttcattcc cagtagaatc
     2341 atgtaagcat ttctgccttt tgtttcaata aaatagttaa ctccatcggc atagcgctga
     2401 cacaaccttt tataataggg cgatagtttt ttggtatatt cctttgccct ttcataaaat
     2461 cctaccgccc gcaaaaagta atctttactc aaagcttctt caccagcaaa ttcgctgatg
     2521 cgcccctgtc caactcgttt tgtgaattcc atctgaaaaa gcctgtcctg cgcattgaca
     2581 taaccccacg caaagtacag atcatccatg ttttgtgctg taatggttgg cacggcaaat
     2641 ttattgcgct cgattgttac cggagaagca agcccgggta cttgtaaact agaggtataa
     2701 tccggcaagc ctgttctcaa aaataaataa atgacagcac caagaatcac tataacaact
     2761 accagtataa tgattatctt tttcattgca aacccccatt gtttcatctg catctattat
     2821 attatagagt aatgctatgg aaatgtcaat caatacatgt tagacaatag aacatgcaat
     2881 cccctctttt ttacagcgat acgctatctc atgacttgct gaaaaaaaat agctgttatc
     2941 acaatactgt tccagcgcat attttttata cacactatct gcataatgat aattcatttt
     3001 atctacatat acataatcaa cgctaccagc aagcatatca ataagatttt cgcaacccgg
     3061 caacataggt gcaatcatga cgtacgttcg tatgccatgt tttttcaact gttttattgc
     3121 atctatcctg tctgctattg cagttgattg cggttcaaaa agtttccgaa tactatcatt
     3181 agcagttgtt atggataatc caacatcaca tgaagatgca ttcttaataa tatccacatc
     3241 tctcagcaca agatttgatt ttgtctgtat tatgacaggc caatcatttt caattagtat
     3301 cgttaagcat gctcgtgtta ttgcatattt tttttctaat ggctggtagg gatcacaaac
     3361 cccgcttatc cataccagtc cctttttctt ctttgcaatt tccttttgca ataacactgc
     3421 tgcattgtct ttactatcaa caaatgttcc ccacggctcc ttatgcccgg taaacttttt
     3481 catgtagcgg gcataacaat ataaacaacc aaattggcat cccacatagg gatttaccac
     3541 ataatcatat acctttgaaa ccgacaatat actttttgcc tgtatgttgc gtataaccat
     3601 actattgctc aataataatg taaataatgt aatttctgat ggcactcatc attattatta
     3661 cacacctgca atcatcgtta ttttgaacag cactatatct ataacccatt acccctgtat
     3721 caaaaaaata ccactatcac aataaaattg taccatatca atgtttaaag tctcaataaa
     3781 aaaagcgt
//
LOCUS       DAOSZP010000164         3770 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_19601_length_3770_cov_2.945087, whole genome shotgun sequence.
ACCESSION   DAOSZP010000164 DAOSZP010000000
VERSION     DAOSZP010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3770
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19601_length_3770_cov_2.945087"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..380)
                     /locus_tag="PK348_04670"
     CDS             complement(<1..380)
                     /locus_tag="PK348_04670"
                     /inference="COORDINATES: protein motif:HMM:NF013909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HXK65539.1"
                     /translation="MHSIKGLFDYLEKVFPLEYQEEYDNSGRQISFDNKPIKGILVAM
                     DVSPVVINEAHENNCNCIITHHPIFFKPIKNILDGNSTGQMVIACIQHEISVYAAHTN
                     ADAVCWDLLAKSLGLKNIALLFPD"
     gene            complement(373..888)
                     /locus_tag="PK348_04675"
     CDS             complement(373..888)
                     /locus_tag="PK348_04675"
                     /inference="COORDINATES: protein motif:HMM:NF021625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate synthase family protein"
                     /protein_id="HXK65540.1"
                     /translation="MAIVNNEEKIHVVEEIQKAFSLLHAAKCGRMFLSGASNIAYTLP
                     YVTDTSDIAMFPGGIIIYNDSIFRVKDPAFGVDHPMVLFMQKITPMHYRAAINIKNEI
                     EYKINAQALGIPVYTSLNIDNLQKTNISENNRIVCVEIKQDNMLYLIGNTPTEIAETL
                     LKINKTEHTHA"
     gene            complement(892..1674)
                     /gene="thiD"
                     /locus_tag="PK348_04680"
                     /pseudo
     CDS             complement(892..1674)
                     /gene="thiD"
                     /locus_tag="PK348_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018132093.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
     gene            1844..2191
                     /locus_tag="PK348_04685"
     CDS             1844..2191
                     /locus_tag="PK348_04685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65541.1"
                     /translation="MNNTPFSCKTSDRWTVIAQDTKYWRVGIYHPEFSSADQITQLEK
                     HSCPELFICANAPCGILLMVDGKEIQYIMNPNDAIMVTEYHNGFRVDPEGYFIVVEQT
                     DFTTEFIERKTTW"
     gene            2185..3024
                     /locus_tag="PK348_04690"
     CDS             2185..3024
                     /locus_tag="PK348_04690"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF001319.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HXK65542.1"
                     /translation="MVINAHTSVYCILGNPVRHSLSPAMHNAAFTHTGINAVYVAFEP
                     ASIKQAFDAIKALGIKGASITIPFKVDVLPLCDSVDTLASRIGSVNTLVNADGMIQGY
                     NTDGYGLLRSLETHSIDYHNKKVLIIGNGGSARAIAYTLARYNAHITICGRNSNNITQ
                     LANDLRKYFTNIETILIDKLTPDITAHNNIIINTTPVGMNPDISRSPIDISLLHPHNV
                     VVDIIYSPLRTQLVQNAQEKGCTVITGDYMLLFQACMQFELWTGKTAPIDIMHQALKQ
                     ALQ"
     gene            3027..>3770
                     /locus_tag="PK348_04695"
     CDS             3027..>3770
                     /locus_tag="PK348_04695"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HXK65543.1"
                     /translation="MLPHYLQSLANNESHASNFTNYSPDTIVAVLNLLGNPQHKLPCI
                     HIAGTNGKGSTAHMIAAILQAAGYTVGLYTSPHLIRYNERISINGKEITDEEISEYTE
                     MLETLCNTHSYTPTFFDAFTMIAFLHFYNSVDIAIIETGLGGRLDSTNIVTPLISVIT
                     PISYDHTAILGSTLHEIAYQKAGIIKKHIPVISATQKPEAKEVLQKEASMKQSPIFFV
                     GDHIRFTIVSQNPLVFNVMVNHSAFDQNKI"
BASE COUNT         1222 a          713 c          608 g         1227 t
ORIGIN      
        1 ttatcaggga acaataatgc aatattctta agacccagtg attttgcaag taaatcccag
       61 caaaccgcat ctgcattggt atgagcagcg tatacactta tttcatgttg aatacatgct
      121 attaccatct ggcctgtact attaccatcc aatatatttt ttattggctt aaaaaatatt
      181 ggatgatgcg ttatgataca attacaatta ttttcatgtg cttcattaat aacaacagga
      241 gatacatcca tagcaacaag aatgcctttg ataggtttat tatcaaatga aatttgcctt
      301 cctgaattgt cgtattcttc ttgatattcc agtggaaata ctttttcaag ataatcaaaa
      361 agccctttta tactatgcat gtgtatgttc cgtcttgtta atttttaata acgtttcagc
      421 tatttcagtt ggagtgttgc caataagata aagcatattg tcctgcttta tttcaacaca
      481 tacaatcctg ttgttttcag aaatattggt tttctgcagg ttgtcaatat ttaatgaagt
      541 gtacaccgga ataccaagtg cctgagcatt aattttatac tcaatttcat tttttatatt
      601 tatagctgcg cgatagtgca tcggagtaat tttctgcata aataatacca tcggatgatc
      661 cacaccaaat gcaggatcct taaccctgaa aattgaatca ttataaataa taattccacc
      721 aggaaacata gctatatcac tggtatcagt aacatacggc agagtatatg ctatgtttga
      781 agccccactt aaaaacattc tgccacattt agcagcatga agtaatgaaa aagctttttg
      841 aatttcctct actacatgta tcttttcttc attatttact atcgccacaa gatgattggt
      901 tggaccatga cctttgccca atggcagaga aaatgtgatg gcctttgtga taaattcttt
      961 tgctttcgtt attgcttcta tggtattgta cccctttgca acataggttg caattgctga
     1021 tgaaaatgtg catccagtgc cgtgggtatt ttttgtctca atacgcttat ttttcaaaac
     1081 tgtgaaatct ttaccatcat aaataacatc gcttggatcg ctttccagat gcccaccttt
     1141 taccactaca taatgtgcac caaatgaata aatttttttt gcagcatctt tcatatcatt
     1201 aatattttta attgtcatac cagtaagcac ttcagcttca tgaatattag gtgttacaac
     1261 atgagcaaca ggaattaaat gttgaattac tgattgaatg gcatcatctg ataataattt
     1321 tgatcctcct tttgcaacca tgactggatc aacaacacag ttattaattg aatattgttt
     1381 aatttttgct gcaacaagct taacaatatc aggatttgcc agcattccgg ttttagctga
     1441 atgtatggga atatcagatg caactgcatc aaactgtttt tcaatcatgt ccagtggtac
     1501 aggcatatag tcaataaccc ccagtgtgtt ctgggcagtt attgcggtaa tcacagtcat
     1561 accaaaacaa cccagtgcag ttattgtttt taaatcagcc tgaataccag caccaccacc
     1621 ggaatctgat ccagctatag tgagtacatt gctaataaaa gatgaggttt tcatttttat
     1681 atccagttct tatatatcaa taaagggggt ttacatttat cacaatataa ctattttttt
     1741 tcaagaatta tttgttgtaa aaaattactt gtcatttctt ctatctttat caaaatcaaa
     1801 cattattttt gtatcaaaaa tacctttata taaaggtcgc tccatgaaca ataccccatt
     1861 ttcatgtaaa acaagcgata gatggactgt tattgcacag gacacaaaat actggcgtgt
     1921 tggcatatat caccctgaat tttctagcgc tgatcaaatt actcaattag aaaaacacag
     1981 ctgtcctgaa ttatttatat gtgccaatgc accctgtgga atattattga tggttgacgg
     2041 aaaagaaatt cagtatataa tgaaccccaa cgatgccata atggtaactg aataccataa
     2101 cggttttagg gttgatcctg aaggttactt tatagtagtg gaacaaacag actttactac
     2161 ggaatttata gaaagaaaaa caacatggta ataaatgcac atacatctgt atattgtatt
     2221 ctgggtaatc ctgtacggca ttcactttca cctgcaatgc acaacgctgc atttacacat
     2281 actggaataa atgcagtata tgttgcgttt gaacctgctt caattaaaca ggcttttgat
     2341 gcaataaaag cgctgggaat aaaaggtgca agtattacca ttcctttcaa ggtagacgtt
     2401 ttaccgctct gtgatagcgt tgatacttta gcatcacgta ttggctcggt taataccctt
     2461 gtcaacgctg atggaatgat acaaggatat aataccgatg gctatggatt actcaggtca
     2521 cttgagactc attctataga ttatcacaac aaaaaggttt taatcattgg caatggtggt
     2581 tctgcaagag ctatcgccta tacccttgcc cgatataacg cacatataac tatatgtggc
     2641 cgaaatagta acaatataac gcaacttgca aatgacttac ggaaatactt taccaatatt
     2701 gaaacaatac tgattgataa gcttacacct gacataaccg cacataataa cattattata
     2761 aataccacac ctgttggcat gaatcctgac atatccagat caccaattga tatatcattg
     2821 ctgcatcctc ataacgtagt ggtagatatt atttattcgc ctttgcgtac tcagcttgtt
     2881 cagaatgcac aggaaaaagg atgtactgta ataactggcg actatatgtt attattccag
     2941 gcatgcatgc aatttgaact ttggacaggc aaaactgcac ccattgacat tatgcatcaa
     3001 gcactaaaac aggcattgca ataactatgc tgccacacta tttacaatca cttgccaata
     3061 atgaatctca tgcaagtaat tttactaatt attctcctga tacaattgtt gctgtattga
     3121 atctgttggg caatcctcag cacaagctac catgcataca catagctggg accaatggga
     3181 aaggttcaac tgcacatatg attgctgcca tacttcaagc tgcaggttac actgttgggt
     3241 tgtacacatc acctcatctt atacgatata atgaacgtat atcaataaat ggcaaagaaa
     3301 taacagatga agaaatatct gaatatacag aaatgctgga aacactatgt aacacacata
     3361 gttatactcc aacgtttttt gatgcattta ctatgattgc ttttcttcat ttctataatt
     3421 cagtggatat tgctatcatt gaaacggggc ttggtggtag acttgattca acaaacattg
     3481 ttactccact tatcagtgtt ataactccta tttcatacga ccatacagca atactgggat
     3541 ctaccttaca tgaaattgca tatcaaaaag caggaataat aaaaaaacat attcctgtta
     3601 taagtgccac acaaaaacct gaagcaaaag aagttttaca aaaagaagca tcgatgaagc
     3661 aatcaccaat attttttgtt ggagatcata tacgctttac tatcgtttca caaaaccctc
     3721 ttgtctttaa tgttatggtt aatcattctg cttttgacca aaataaaatt
//
LOCUS       DAOSZP010000165         3768 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_19612_length_3768_cov_3.354430, whole genome shotgun sequence.
ACCESSION   DAOSZP010000165 DAOSZP010000000
VERSION     DAOSZP010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3768
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19612_length_3768_cov_3.354430"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(389..1021)
                     /gene="nth"
                     /locus_tag="PK348_04700"
     CDS             complement(389..1021)
                     /gene="nth"
                     /locus_tag="PK348_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006000519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HXK65544.1"
                     /translation="MIDKATSKKIINRLKKQYQVSAPLHFNNLYQLCIAVVLSAQTTD
                     NQVNRVTPLLFKKYPDFYSLAKAHIDDVEKIIKSTGFYHNKAKHIVALAGTIVHTFNG
                     VVPETREELMSLPGVGRKSANVILAFGFGIPAIPVDTHVSRVASRIGYANSRKPIAIE
                     MAMMESIRKSDWIIAHLLFIYHGRTICHARKPACSRCPIILYCHFENKNL"
     gene            complement(1011..1541)
                     /locus_tag="PK348_04705"
     CDS             complement(1011..1541)
                     /locus_tag="PK348_04705"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXK65545.1"
                     /translation="MKKCLLFIICLTGILLYYCSSVQYGKHKSLWRFNDPYINQTIIN
                     AWENVYKRRYEWAALDFTRLIQKNYIDYDILFGAGLSYYFMNESKKAMDYFTQAIEKN
                     PHHFEAYYYRAKLFLSQGAHAKAKQDLEAIIYMKYDEPIICGYYFTNDDIADIHVFKS
                     RQKEAQSLLMAIAHDR"
     gene            complement(1538..2137)
                     /locus_tag="PK348_04710"
     CDS             complement(1538..2137)
                     /locus_tag="PK348_04710"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012031963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HXK65546.1"
                     /translation="MEIVVATRNKGKIKEILSFGIPSIIFTSLEKYPEIGEIEETGST
                     FTDNALLKARHVCNSTGLPALADDSGLVVDALNGEPGVYSARFGNLKTDEERYMLLLQ
                     KMQDVPDDRRHARFVCVMALVFPDGREYTEEGICEGIIAKEPAGENGFGYDPVFYIPE
                     LGKTMAQLPLDVKNAISHRARALQKVKILLEKIVGEKTI"
     gene            2306..2980
                     /locus_tag="PK348_04715"
     CDS             2306..2980
                     /locus_tag="PK348_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65547.1"
                     /translation="MEDQKQLNILRIGIANLPELEELVKSFRLMNQYSKRRRFVVSRE
                     DLKDIYGNIIVEKAHDINISVVKLLQRNFKPDTDFKIFSSDEGIAIVTNTESPNAKEF
                     SSQLISTIEGIGGGIYKPFIDTVPGFYELFKLFEKGLSPKLVVVGYIPVKQVAQEVSM
                     YNEIKKYDPYIRILDITHDELKPKSFLKGAVTFHFDNAHKVWQRFLTKLIQEYTKPYF
                     MEQITK"
     gene            2992..>3768
                     /locus_tag="PK348_04720"
     CDS             2992..>3768
                     /locus_tag="PK348_04720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HXK65548.1"
                     /translation="MKDKLLQFFAITLFIIVIAVTTSITISMYFINVHSWVFYLSIVC
                     TMLLIVLFVYIRIIHKILFIDKIEKLHAIAENFKKGEFHHKVGQIPGDDPFAKIYTEL
                     LSASRNLENIIKSQKSEIEKLRELYTNMMFSMNAYIVVLDIHNTILFTNDLFLKKFQY
                     TPQEVSGKNITELFYFLTGTVLQAIEEIKKTHESIVLEKAHLISNNRITVIADIKLSY
                     MISNNEPQIIMVMDDVTSKTKRDYQISLITRLSEKIQKDDV"
BASE COUNT         1228 a          705 c          615 g         1220 t
ORIGIN      
        1 agcaccaaca tgaaccagta cagttaatgc agcatcacta taccatttaa ttcaattatt
       61 gattatgcat atactatatc attaaaacaa aatgaacaat tacaatctgc cagtcacctg
      121 gcgcgtctgg ctgcgcgatt tgctgatggt aatgaaaaaa cgattatatc aatgtttgag
      181 aaagggaata tttcattaaa aatctggata atgagtgtaa tgggatttca caaaaaaatt
      241 atatatctgc ctgctttgct accatactgt gcagacacaa acaaaacatt agctacctct
      301 gcttacagca ctctggtaca gataacaggt aaaaatcctt ctgctgaata caacttgcca
      361 accaatgacc cacaggtcct aaaagctttc aaagattttt attttcaaag tggcaataca
      421 aaataatggg gcatctgctg catgctggct ttcgtgcatg gcatattgtc cttccatgat
      481 agatgaatag taaatgtgct ataatccagt ctgatttcct gatgctttcc atcatcgcca
      541 tttcaatagc aataggtttt ctgctatttg cataccctat tctactggca acacgggata
      601 catgggtatc aacgggtatt gcaggtatgc caaaaccaaa agccaatatc acattggcag
      661 attttctgcc cacccccggc aggctcatta gttcttcacg tgtttcaggt acaactccat
      721 taaacgtatg tacaatagtt ccggccaatg caacaatatg ttttgccttg ttgtgataga
      781 aacctgttga tttaataatt ttttcaacat catcgatgtg agcctttgcc agtgaataaa
      841 aatcaggata ttttttaaat agtaatggag ttaccctgtt aacctgattg tcagttgtct
      901 gagcagacaa caccactgca atacacaact gatatagatt gttaaagtgc aatggtgcag
      961 aaacctgata ctgcttcttc aaacgattaa tgattttttt tgaggtagct ttatctatca
     1021 tgagctatcg ccataagaag tgattgagcc tctttttgcc tggacttgaa tacatgtata
     1081 tctgcaatat catcattagt aaaataatat ccacagataa ttggttcatc atatttcatg
     1141 taaataatag cttccagatc ttgttttgcc tttgcatgtg caccctgact taaaaatagt
     1201 tttgccctgt aataatacgc ttcaaagtga tgggggtttt tttcaatagc ctgtgtaaaa
     1261 taatccatag cttttttact ttcattcata aaataatatg atagtccagc cccaaacagg
     1321 atatcataat cgatgtagtt tttctgtata agccgtgtaa aatccagcgc agcccattca
     1381 tatcggcgct tatatacatt ttcccaggca ttgataattg tctgatttat atacgggtca
     1441 ttaaaccgcc agaggctttt atgcttaccg tattgcacgg atgaacagta gtaaagcagg
     1501 atacctgtaa ggcatattat aaatagtaaa cattttttca tattgttttt tcacctacaa
     1561 tcttttctaa caggatttta accttttgta aagcacgtgc tctatggctt attgcatttt
     1621 ttacgtccag aggtaattgc gccatagtct ttcccaattc aggtatataa aaaacaggat
     1681 catatccaaa accattttca cctgctggct cttttgcaat aataccctca catatacctt
     1741 cttctgtata ttccctgcca tcggggaaga ccagagccat aacacataca aagcgtgcat
     1801 gccgcctgtc atcaggcaca tcctgcattt tttgtaataa tagcatgtat ctttcttcat
     1861 ctgttttaag attaccaaat cttgctgaat acactcccgg ctcaccattc agtgcatcca
     1921 ctacaagccc tgaatcatcc gcaagagcag gcaaccctgt actattgcat acatgtcgtg
     1981 cctttaataa tgcattatca gtaaaggttg agcccgtttc ttctatttca cctatttcgg
     2041 gatatttttc aagtgaggtg aatattattg aaggaatacc aaaagaaagt atttctttta
     2101 tttttccttt attgcgcgtt gcaaccacta tctccatatt tgttaaatac tcctccaaaa
     2161 actattcaat gactttgttc tttacataaa aattattgat tatccattaa atgctagact
     2221 aaaatagtca atcgaaaata ttggtggctg tttttttatt tattttcatt gaaaatttta
     2281 ttatcattat atatagttca aagccatgga agaccagaaa cagttgaata ttttaaggat
     2341 aggtattgca aatcttcccg aactggaaga gctggtaaag tcttttcgcc tgatgaatca
     2401 gtattcaaag cgacgacgtt ttgtagtttc acgagaggat ttaaaagata tctatggtaa
     2461 cattattgtt gaaaaagccc acgatataaa catatcagtt gtaaaattac tgcaacgaaa
     2521 ttttaaacct gatacagact ttaaaatatt cagttctgat gaaggcattg ctattgtaac
     2581 caatactgaa tcgcccaatg caaaagaatt ctcttcacaa cttatctcta ccattgaagg
     2641 aattggggga ggaatctata aaccatttat tgacaccgta ccaggcttct atgaactttt
     2701 caagttgttt gaaaaaggtc tttcgccaaa attagttgtg gtgggctata taccggtaaa
     2761 acaggttgcg caggaagtat ccatgtataa tgaaataaaa aaatatgacc cctatatcag
     2821 aatacttgac attacccatg atgaattaaa acctaaatca tttttaaaag gggctgtaac
     2881 ctttcatttt gacaatgccc ataaagtatg gcaaagattt ttaacaaagc taatacagga
     2941 atatactaag ccatatttca tggaacaaat cacaaaataa gcaatgatgg tatgaaagat
     3001 aagcttttac aattttttgc tataaccctg tttataatcg ttattgcagt tacaacaagc
     3061 attaccatat caatgtactt tataaacgtg cattcatggg ttttctattt aagtattgtc
     3121 tgcactatgt tacttattgt tctttttgtc tatatcagga taatacacaa aattctattt
     3181 atagataaaa ttgaaaaatt acatgcaatt gctgaaaatt ttaaaaaggg cgagttccat
     3241 cacaaagttg gccagatacc cggcgatgat ccttttgcaa aaatatatac agagctgtta
     3301 tcggcttcca ggaatctgga aaacattatt aaatcccaga aaagcgaaat agagaagtta
     3361 cgtgaactgt ataccaacat gatgttttcc atgaatgcct atattgttgt acttgatata
     3421 cacaatacca tcctttttac caacgattta tttttaaaga aatttcaata cacgcctcaa
     3481 gaagtatcgg gaaaaaatat tactgaactt ttttattttt taacgggtac agtgctgcag
     3541 gccattgaag agataaaaaa aacgcatgaa tccatagttt tggaaaaagc ccacctgatt
     3601 tcaaataacc gtattactgt tattgctgac attaaattgt catatatgat ttccaataat
     3661 gagccgcaga taattatggt aatggatgat gttaccagta aaacaaagcg agattatcag
     3721 atttcactta ttaccaggct atcagaaaaa attcagaaag atgatgtg
//
LOCUS       DAOSZP010000166         3766 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_19625_length_3766_cov_2.794126, whole genome shotgun sequence.
ACCESSION   DAOSZP010000166 DAOSZP010000000
VERSION     DAOSZP010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3766
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19625_length_3766_cov_2.794126"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            96..1001
                     /locus_tag="PK348_04725"
     CDS             96..1001
                     /locus_tag="PK348_04725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65549.1"
                     /translation="METTFYSLATKASFNKALNHFYSVNDVVDEVEGGRNFADVVNIM
                     LSEGDLMVDQVLPMVGAILRDKYQYTFDSYNVPVTMNDFSKIVEETSKWTALDIVIVY
                     FNVNGEPILINPKNTAHWERVREIVRDQLMVIYTKKKKEANPKVENEAINAIEEMIAG
                     KDVFINKEFIDETIAVKEVKKPTPAAVAAAGKKNMTPKYAVTVTNELFHNGNVEAWKR
                     IIESFKVSHPDLDVLIFHEGEKINDINSLFKWGKVKHGDPIFFQVVGENIRDVSKLQK
                     YLFEGASPRFEQFLKIAVGKVLNLF"
     gene            1036..3636
                     /locus_tag="PK348_04730"
     CDS             1036..3636
                     /locus_tag="PK348_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HXK65550.1"
                     /translation="MENIVFFNKDFTGVDDKQYKKLGIRGRRAADLAKMGLPIAPGFI
                     IDADLTMKLPKVNVKQLIKTFVDKIEKDMKKGYGDSEKPLLLKVVLSSDLNVPFFPSI
                     HNVGLNDKVVTGFAKFTGEDFAWGEYRFLLRSIASKIYGIDEKELDELEGKLQKNAKA
                     KEYKEVVAKYKKFLGDKFSEDVYDQLGLILKGAAARYCDSDIDVDNSLSIMIQAMVYG
                     NFGQNSFAGNYFTRNIINGDALIQGEFLQNEFDVDRGTPKDIAKIDKKYYDKLVEIAK
                     KVEDNFKEIRNIKFTIEEGNFWLIEQREVDEKSTQAHIKTLLDLHTRKVVTPEFLIES
                     IKPSQLNELLHPVIDPRTTKNVKSIKGGIAGSTGAAIGRVFFSTDRLLEEYKRAVMHG
                     GDTNLILVMPATYAEDVKAIEVARGVITSEGGFSSHAPVVARSLGKVAMVQPEMKIRG
                     NSFTLAGKTIKEGDYVSINVPYYEPPTIYLDKVGLIEPDFEKNGLTDFLTIVQKFIKD
                     FDVRANADQPRDARVAKMFFADGIGLCRTEHMFFNEKRIMKFREMILSETEAERRKAL
                     DDLKPMQRGDFYELFKIMQGHPVTIRLLDAPLHEFLPRTEDSMKEFIQYMKSRKKGMS
                     DAEIRARCEELSEMNPMLGHRGCRVAITYPEIYEMQCRAIFEAACMLKKEGINVVPEI
                     MIPIVMTETEIKFIKNGKKIEGKTVKGIRDIKDEVVEEYGIDDLEYEVGTMIELPAAA
                     VGAGRIAQYAEFFSFGTNDLTQTTYGLSRDDINSFFPSYSLYDLIPNNTFKVLGREVK
                     ELIEIAATRGKLTRPDITMGLCGEHGADPDNIEFCMNVGLNYVSCSPYAIPLSKLAIA
                     QLNIKQAKSK"
BASE COUNT         1242 a          591 c          850 g         1083 t
ORIGIN      
        1 gtaaaaaatc aaaaaaatat tttatttttg attattttat atatagctat tgcgttttgt
       61 gcattcataa taaattttca aaaagaggga aatccatgga aacaacattc tattcattag
      121 ctactaaggc tagttttaac aaggctttaa atcacttcta tagtgttaat gacgttgttg
      181 atgaggttga ggggggtaga aattttgctg atgtggtaaa catcatgctt tctgaagggg
      241 atttgatggt ggatcaggta ttgcccatgg ttggggctat tttgcgtgat aaataccagt
      301 atacgtttga ttcatataat gtgccggtaa ccatgaatga tttcagcaaa atagttgaag
      361 aaacatctaa atggacagcc cttgatattg tcattgtata tttcaacgtc aatggtgagc
      421 caatccttat taatcctaaa aatacagccc actgggaaag ggttcgtgaa attgtccgcg
      481 atcagcttat ggtcatttat acaaagaaga aaaaagaagc aaacccaaag gttgaaaatg
      541 aagcaataaa tgctattgaa gaaatgattg ctggaaagga tgtgtttatc aataaagaat
      601 ttatcgatga aacgattgct gttaaagaag tgaaaaagcc aactccagct gccgtggcag
      661 ctgccggcaa aaagaatatg acgccaaaat acgctgttac tgttaccaat gaactgttcc
      721 acaatggtaa tgttgaagca tggaagcgta taattgaaag cttcaaagtt tcacatcctg
      781 acctggatgt gttgatcttc catgagggtg aaaaaatcaa tgatattaac tcactgttta
      841 agtgggggaa agtaaagcat ggtgatccca tattcttcca ggtagttggg gaaaatattc
      901 gggatgtgtc taaattacag aagtacctgt ttgaaggtgc atcgccacgg tttgaacagt
      961 tcttgaagat agctgttggg aaagtattaa atcttttcta ataccatatg aaacaaatta
     1021 aaaagagagg gtagtatgga aaatatagtt tttttcaata aagattttac cggtgttgat
     1081 gataagcaat acaagaagtt aggtatcaga ggccgcagag ctgctgattt agcaaaaatg
     1141 ggattaccga tagctccggg atttattata gatgctgatt taaccatgaa attaccaaag
     1201 gttaatgtaa agcaattaat taagaccttt gttgataaaa tagaaaaaga catgaaaaaa
     1261 ggatatggcg atagtgaaaa gccattgctt ttgaaagtgg tattaagctc agatttaaat
     1321 gttccttttt tcccatccat tcacaatgtt ggtttaaatg ataaggtcgt tacaggtttt
     1381 gccaaattta ccggggaaga ttttgcctgg ggcgaataca gatttttact tcgcagcatt
     1441 gcttcaaaaa tttacgggat tgatgaaaaa gaactggatg aactggaagg gaaactccaa
     1501 aaaaatgcaa aagctaaaga atataaagaa gtggtagcta aatataaaaa gtttttaggc
     1561 gacaagttca gtgaggatgt gtatgaccag ttgggtctaa ttcttaaagg tgctgcagca
     1621 cggtattgtg actcggatat tgatgttgac aattcattgt caattatgat tcaggcaatg
     1681 gtgtatggta actttggaca aaattccttt gcgggaaatt attttacccg caatatcatt
     1741 aacggtgatg cactcattca gggcgaattc ttgcagaatg agtttgatgt tgaccgtggt
     1801 actccaaagg atattgctaa gattgataaa aagtattatg acaagttagt tgagatagct
     1861 aagaaagtgg aagataactt taaagaaata cgtaacataa aatttaccat tgaagaaggt
     1921 aatttctggc tcattgagca gcgcgaagtt gatgaaaagt caacacaggc acatatcaag
     1981 acattgcttg acctgcatac ccgtaaagtt gtaacacctg aatttttaat tgaatccata
     2041 aaaccaagcc agcttaacga acttttgcac ccggtgattg atccacggac aacgaaaaat
     2101 gtgaaatcta taaaaggtgg gatagctggt tcaaccggcg cagctattgg tagggtattc
     2161 ttttcaaccg acagactgct tgaagaatat aaacgtgcgg tgatgcatgg gggtgacact
     2221 aaccttatac tggttatgcc agcaacctat gctgaggatg ttaaggcaat tgaggttgca
     2281 cgtggtgtta taacatcaga aggtggtttt tcatcgcatg ctcctgttgt tgcacgaagc
     2341 ttgggtaaag ttgctatggt acagcctgag atgaagatca ggggtaactc atttaccctg
     2401 gcaggtaaaa ccataaaaga gggcgattat gtttcaataa atgtgccgta ttatgagcct
     2461 cctacaattt atttagataa ggtaggctta atagaaccag attttgaaaa gaatgggtta
     2521 acagactttt taacaatagt gcagaaattc attaaagatt ttgatgtgcg tgccaatgct
     2581 gaccaaccac gtgatgccag agtggcaaag atgttctttg ctgatggtat aggtttatgc
     2641 cgcactgaac atatgttctt taatgaaaag cgcatcatga agttcagaga gatgatatta
     2701 tcagaaactg aagccgaacg acgaaaagca ctggatgacc tcaaaccaat gcagaggggt
     2761 gacttctatg aattgtttaa gataatgcag ggtcatcctg taaccatacg gttgcttgat
     2821 gctccattgc atgaattcct gcccagaaca gaagacagta tgaaagagtt tatacagtac
     2881 atgaaatcgc gtaaaaaagg aatgtcagat gctgaaatac gtgcgcgatg tgaagaacta
     2941 tctgaaatga atccaatgct tggacaccgg ggttgccgtg ttgctatcac atatcctgaa
     3001 atttacgaaa tgcagtgcag ggcaattttt gaagctgcat gtatgctgaa gaaagaaggc
     3061 atcaatgttg tccctgagat aatgatccct atcgtcatga ccgagacaga aatcaagttt
     3121 attaagaatg gcaagaagat tgaagggaaa acggtaaaag gtattcgcga catcaaggat
     3181 gaggtcgttg aggaatatgg tattgatgat ttagagtatg aggttggtac aatgattgaa
     3241 ttacccgctg cggcggttgg tgctggcaga attgcacagt atgctgagtt ctttagtttt
     3301 ggtaccaatg acctcacaca gactacatat ggattatcgc gagatgacat taattcattc
     3361 ttcccaagct attcactgta tgaccttatt cccaataaca catttaaggt tctgggaaga
     3421 gaagtgaaag aacttataga aatagctgca acacgcggta agcttactcg tcctgatata
     3481 accatggggc tgtgtggaga acatggtgct gatcctgata acatagaatt ctgtatgaat
     3541 gtgggattga attatgtatc gtgctcacca tatgcaatcc cactatctaa gttggcgata
     3601 gctcagttga atataaaaca ggcaaagagt aaataataca gtgatgatgt cttatcagta
     3661 actatcgccc atatgattgt aaatccatta aggctgctta cacaaaagca gccttgttat
     3721 tttccgcaaa ctttgcatgc aggattttgt tccagtgaaa tagatg
//
LOCUS       DAOSZP010000167         3756 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_19703_length_3756_cov_3.683059, whole genome shotgun sequence.
ACCESSION   DAOSZP010000167 DAOSZP010000000
VERSION     DAOSZP010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3756
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19703_length_3756_cov_3.683059"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            153..599
                     /locus_tag="PK348_04735"
     CDS             153..599
                     /locus_tag="PK348_04735"
                     /inference="COORDINATES: protein motif:HMM:NF014543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65551.1"
                     /translation="MKIIKYLTLILFIFSLSCASAPKNCKEGDCNNGVGTTIHENGSY
                     KGNFKNSTREGLGEYTFNNGDTYRGNYKNNLRNGEGEYKWKNGDSYRGNWVDGKRNGF
                     GIYTWSDGTRYEGEWKDDARHGKGKLILKDGTTFDGMWENDAYIRK"
     gene            635..967
                     /locus_tag="PK348_04740"
     CDS             635..967
                     /locus_tag="PK348_04740"
                     /inference="COORDINATES: protein motif:HMM:NF014176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HXK65552.1"
                     /translation="MNKRNVLMVCCIALLIPAFIFAQGVVKVKKTSAAPKQGTVTFDH
                     QIHKSKGITDCKTCHHTGNTAKKCGDSGCHWETQNKGYNAIHSKCLGCHRTKGGKAPT
                     LCTQCHKR"
     gene            1012..2067
                     /locus_tag="PK348_04745"
     CDS             1012..2067
                     /locus_tag="PK348_04745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65553.1"
                     /translation="MPGLITHQRIFFESVKSLKKQKHPNYATHLLDIQFTNPRFTSSA
                     MAGLLVPNLFDYIPGKRHIFFGNSIKHLLHSDGGIPIIHYMFSTILKHSSHEPDEWLY
                     YQKGFFYGYVSHWVVDALFHPFVFYWSGFPTFKDNRISLYRNQHLLFEYTIDQYMAYF
                     FNSEPFDYNLKAMLPVKGSVQYKAIKDLISGAIAHTNTSLKINIPLFPYTPLDTFDIA
                     VKCVPFVYMIKNNRSPQFVRILRFLEKRFYSDFLVRYPDGRRINRHILNLHRERWFIP
                     TGEPGLHYESVDDLFRIARDKTVFIWKQLEELFSKQEHITVQQLNTVLEYSNAYTGKK
                     GFSIDKMTLQNPIRLRN"
     gene            complement(2071..3153)
                     /locus_tag="PK348_04750"
     CDS             complement(2071..3153)
                     /locus_tag="PK348_04750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65554.1"
                     /translation="MGSFTVKKKGLLLLLLLLWPLSILGYEVNISSVRPNLMNIRTTN
                     GKLYAYIVLLFNENPHFVNLIQADSIPVRLMQTQGFNVMQYDAYIRTLQDAPLKGLAQ
                     QILQVYKEPDKITDTELEQLTQKLENRNIILRFSKTKDGNGSRYLLDYCIFGKRKPVT
                     INHPLFNVKEKIFNIQPLIYYDEFSTSNSTFYFDMIYINPDEVYNDYIISKRIIEGKS
                     CENMFFVGAHVTEDIKYCIKKAFASKETIFDEIWRMFEIHELTHKILNNYYYFFDQVT
                     GEELALSSTIYYNTYLGLAILYSYLDYNTINPHRIAATNFIKFLSLYTANSEYIEKPS
                     LIKQIPLEDIKKIAKLHFETTIKNLK"
     gene            3383..>3756
                     /locus_tag="PK348_04755"
     CDS             3383..>3756
                     /locus_tag="PK348_04755"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXK65555.1"
                     /translation="MKLLAQGTINDYCQVIGHIGYPAYIVKGHHKIMMIDAGINLLAP
                     KYYTDLSTILGDPQKLNYAAITHSHYDHLGAIPYLLKKIPGLTIVGAERIQQLLAKQS
                     VIDFMTHLSNIQRPLFADITGQ"
BASE COUNT         1234 a          688 c          646 g         1188 t
ORIGIN      
        1 agtatttatt tcatcctgca atctactcgt atattgaact atcgcccata cccatttttt
       61 taaaaaacta tttgcgtaat attaattcat tttatacaat gcctacagta aaaatactct
      121 atagagttat ctcacagtaa gggaggagca gcatgaaaat aataaaatat cttacactta
      181 tactattcat attttcttta agctgtgctt cagcaccaaa aaactgcaaa gagggtgatt
      241 gcaacaatgg agttggcaca accattcatg agaatggttc atataaggga aattttaaaa
      301 acagtacccg tgaaggtctt ggcgaatata cattcaacaa tggcgatacc tatcggggca
      361 attataaaaa caacctgcgt aacggcgaag gtgaatacaa atggaaaaat ggcgatagtt
      421 atcgaggcaa ctgggttgat ggtaaaagaa atggttttgg tatttatacc tggtctgacg
      481 gcacacgata tgaaggggaa tggaaggatg atgctcgcca tggtaaaggc aaacttatac
      541 tcaaagatgg gaccaccttt gatggcatgt gggaaaatga tgcgtatatc cgaaaataat
      601 tttaatgata atctaaacat ttttaggagg ttatatgaat aagaggaatg tgcttatggt
      661 ttgttgcatt gcactcctga ttccagcatt tatctttgca cagggtgtgg tgaaagtaaa
      721 aaaaaccagt gcagcaccta aacagggaac tgttacattt gatcaccaga tacataaaag
      781 caaaggaatt accgattgca aaacatgcca tcataccggc aatactgcaa aaaaatgcgg
      841 tgatagtgga tgccactggg aaacacagaa taaaggatat aatgccattc acagcaaatg
      901 tttaggatgc cacaggacaa aaggtggcaa agcccctaca ctatgtactc aatgccataa
      961 acgataacac aatatctggg ggattgttaa atcccccaga catgtattca tatgcctgga
     1021 ttaataaccc atcaaagaat tttttttgaa tctgttaagt cattaaaaaa gcaaaagcat
     1081 cccaattatg caacacatct tctggatata caatttacca atccccgttt tacttcctct
     1141 gccatggcag gactgcttgt cccgaacctt tttgattata tcccgggaaa aagacatatt
     1201 ttttttggga attctataaa acacctgtta cattccgacg gtggtatacc catcattcat
     1261 tacatgtttt ctaccattct caagcattca tcacatgaac ctgacgaatg gctgtattat
     1321 caaaaaggtt ttttctatgg atatgtttca cactgggtgg tcgatgcact gtttcatcca
     1381 tttgtatttt actggtcagg attcccaaca ttcaaagaca acaggatctc actctataga
     1441 aaccagcatc ttctctttga atatactatt gaccagtaca tggcgtattt ttttaattct
     1501 gagccatttg actataatct gaaggcaatg ctgcccgtta aaggttcagt tcagtataaa
     1561 gccatcaagg atttaatttc gggagctatc gcccatacca atacttccct gaaaataaat
     1621 ataccattgt ttccctacac accgctggac acatttgaca ttgcagtaaa atgtgtgcct
     1681 tttgtgtaca tgattaaaaa taatcgcagc cctcagtttg ttcgtattct gcgctttcta
     1741 gaaaaaaggt tctacagcga ttttctggta cgataccccg atgggaggag aattaaccgt
     1801 catatattaa atcttcaccg tgaacgatgg tttattccca ccggtgaacc gggattgcat
     1861 tatgaatcag tggatgacct gttcagaatt gcaagggaca aaaccgtgtt catatggaaa
     1921 cagcttgaag aactgtttag taagcaagaa catattaccg tacaacaatt aaatactgtg
     1981 ctggaatatt ccaatgccta tactggcaaa aaaggatttt caatagacaa aatgactttg
     2041 cagaacccca tcaggttacg caattaacgg ttattttaga ttttttatag ttgtttcaaa
     2101 atgaagtttt gcaatcttct ttatatcttc aagcgggatt tgttttataa gagatggctt
     2161 ttcaatatat tcactattag cagtatacag tgataaaaac ttaatgaaat ttgtagcagc
     2221 aatgcgatgg ggattaatag tgttgtaatc caggtagctg taaagtattg ccaggcccag
     2281 atacgtatta taataaatgg tactgcttaa tgccagttcc tcacccgtta cctgatcaaa
     2341 aaaataatag taattattca atattttatg cgtcaactca tgtatttcaa acatcctcca
     2401 gatttcgtca aaaatagttt ccttgctggc aaaagctttt tttatgcaat actttatatc
     2461 ttcagtaaca tgtgctccaa caaaaaacat attctcgcac gatttacctt caattattct
     2521 ttttgaaata atgtaatcat tatacacttc atcagggttt atgtatatca tgtcaaaata
     2581 gaaggttgaa ttgctggttg aaaattcatc ataataaatt aaaggctgaa tattaaatat
     2641 tttttcctta acattaaaaa gagggtgatt aatggttacc ggttttcttt tgccaaatat
     2701 gcaatagtca agaagatatc tggatccatt gccatccttg gtttttgaaa agcgcaaaat
     2761 aatattccgg ttttctaatt tctgtgtcag ctgttctaat tcagtatctg ttattttatc
     2821 cggctcttta taaacctgta gaatctgctg tgccagacct tttaagggtg catcctgaag
     2881 tgttctgata tacgcatcat actgcataac attaaaacct tgtgtctgca ttagccttac
     2941 agggatactg tcagcctgta ttaaatttac aaaatgcggg ttttcattaa atagtaatac
     3001 tatataagca tacaacttcc cgttagtggt tctaatattc atcaagtttg gacggacgct
     3061 ggaaatgttt acttcataac ccaaaatgct caacggccac aaaagcaata agagcaacaa
     3121 caatcctttc ttttttactg taaaactacc catattgtat ataccttatt ttaaatcaat
     3181 gtagctttgt tatcgttttt atacaaaaaa taattaccat taagcaatat aataaaaata
     3241 tcttttccta ccaaatatta ttgaaaaatt ttatagttat tattttgtag taatagttat
     3301 atattatttt ttttacaaat taaccggttt caattcgtgg gcgatagttc ttagaataaa
     3361 ccttaccgta taagaggtta tcatgaaatt acttgcacag ggaacaatta atgattattg
     3421 ccaggtcata ggacatatag gttatccggc atatattgtt aaggggcatc acaagattat
     3481 gatgatcgat gcaggcatta atcttttagc accaaaatat tacactgacc tttcaaccat
     3541 tcttggtgat ccgcaaaagc tcaattatgc agcaattacc cattcccatt acgaccatct
     3601 gggagctatt ccataccttt taaaaaaaat tcccggatta accatagtag gtgctgaacg
     3661 cattcagcaa ctacttgcaa agcagtcagt tattgatttt atgacacact taagcaacat
     3721 acagagacca ttatttgcag atattacagg tcagga
//
LOCUS       DAOSZP010000168         3755 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_19713_length_3755_cov_2.960811, whole genome shotgun sequence.
ACCESSION   DAOSZP010000168 DAOSZP010000000
VERSION     DAOSZP010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3755)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3755)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3755
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19713_length_3755_cov_2.960811"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1499
                     /locus_tag="PK348_04760"
     CDS             <1..1499
                     /locus_tag="PK348_04760"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HXK65556.1"
                     /translation="KDSSSVVCVCSSKFQTDNVLSKKSELPNLKKIIVCDDINYNDDM
                     VITFKDAVNAGNKNLHVDEIENRTKNVKPDDVMTLIYTSGTTGDPKGVMLTHKNIMFI
                     CLTFNKVEALPEGFIHLSLLPLSHSVERTMDYYSVLERGAVIYYSRGTEYFAEELKEI
                     RPQCDILVPRVFEKIYNGVMAKVKEMPEKKQKIFNWALSVGLQAAPYFMAAKRKPPVL
                     AVKYAIANKLIFSKLKNALGLDRVICWGAAGAPLAKEIHDFFWAMNLQVRKGYGLTET
                     SPVLAVDGSPKIKPIKSDGWICPFPETQIKIADDGEILAKGPQLMKGYLNKPEATKEM
                     FTDDGWIKTGDIGVLDSEGYLKITDRKKDIIVTAGGKNIAPQVIEQRLLIHPFIEQVA
                     IVGDARKYIVALIVPNFETLTQWAATQGISAPSNDELVKHPDVRKKYEAIVNEVNQEF
                     GKVEQIKKFTLLPQQFTQETGELTPTLKIKRKVIQKKYADIIEEMYKE"
     gene            1699..2292
                     /locus_tag="PK348_04765"
     CDS             1699..2292
                     /locus_tag="PK348_04765"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HXK65557.1"
                     /translation="MSHTIIDKRQQILDVAKELFATKGYSATGIREIAEKAGLSLGNF
                     YNYFKNKEDLFKSLLDPQNILEHLTEIKLMLNDGFPFNFKDIIKSIKTVVDSDFHLYR
                     LIFIDLTEFNGMHTDKILDRIITEATATFNANVKNSIVGTVIKDEDYQFYIKAFIIST
                     LSLLVIGNILPSANLNDKSNDYIAEKLSNVILHGILL"
     gene            2289..3074
                     /locus_tag="PK348_04770"
     CDS             2289..3074
                     /locus_tag="PK348_04770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65558.1"
                     /translation="MNYWTHIFFWAIVTILASGLAFAQEPFSLLSVEDKPTLSIKGYV
                     EIDTTTSTYPHQETADIFKKDELKANTTIKYGKESFYVYTDINAYLLPNLINENINPT
                     YRYSEETDILRNGTITSSNAEINFRECYINYEIGSVRLRAGNQIFAWGTADAFNPTSY
                     FNTYDLREFLFKDEDTELKMGVPAVSMLMTLGNDTLELVITPLHIPLRMPQAGSFWQL
                     TVKEGPFPVTIAQPEALPFEIANAGVGARYYHNIAGFDVHLSA"
     gene            3102..3716
                     /locus_tag="PK348_04775"
     CDS             3102..3716
                     /locus_tag="PK348_04775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65559.1"
                     /translation="MPVKTILQPNEPVSILVEPRYAIVNMAGTALSKAYKDFVFQVEA
                     VYSPDKTGVVDVNLQNLSAAQLQTLLPYKTRKSHFVTYSAGFNYFIPIRDYFKEHEGQ
                     CVFTFEWSQSHFFDNKLMKPMLSDIVVLRLQDSFFNDKLDLKISSFVEKQTSSYMIMP
                     RVSYRFDSGLTFDVTYVYLHMTADSYFAGYNNNDCVTGRVSYEF"
BASE COUNT         1221 a          757 c          733 g         1044 t
ORIGIN      
        1 taaaagattc ttcatctgtt gtgtgcgtgt gttcatcaaa atttcagact gataatgtcc
       61 tttctaaaaa aagtgaactg cccaacttaa aaaaaattat tgtgtgcgat gatatcaatt
      121 acaatgatga tatggtgatt acatttaaag atgccgtaaa tgctggtaat aaaaatctgc
      181 acgttgatga aatagaaaac cgtacaaaga acgtaaaacc cgacgatgta atgacattaa
      241 tctatacatc gggaacaact ggcgacccaa agggcgttat gctgacccac aaaaatatca
      301 tgtttatttg tcttacgttt aacaaagttg aagctttacc agaaggtttt attcacttat
      361 cgttacttcc actatctcat tcggttgagc gtaccatgga ttattatagc gtgttagagc
      421 gtggagctgt aatttattat agccgtggta cagaatactt tgcagaagaa ctcaaggaaa
      481 tccgccctca atgcgatatt ctggttcccc gcgtttttga aaagatatac aacggcgtta
      541 tggcaaaagt taaggaaatg cctgaaaaga aacaaaagat ttttaactgg gcactatcgg
      601 taggacttca ggcagcacca tactttatgg cagcaaaacg caaaccacca gtacttgcag
      661 taaaatatgc aattgccaat aaactcattt tctcaaaact taaaaacgca cttggccttg
      721 accgtgttat ttgctggggt gccgctggtg caccgctggc aaaagaaata catgatttct
      781 tctgggcaat gaatcttcag gtacgcaaag gatacgggtt aactgaaact tcacccgttt
      841 tagctgtaga cggttcgcca aaaatcaagc ctataaaatc tgatggctgg atatgtccat
      901 tccctgaaac gcagataaag attgctgatg acggagaaat attagccaaa ggcccacagt
      961 tgatgaaagg ttacctcaat aaacctgaag ccaccaagga aatgtttact gacgatggat
     1021 ggataaaaac aggtgatatt ggtgtccttg acagtgaagg atatctaaaa ataactgacc
     1081 gcaaaaaaga tattatcgtc acagctggcg gtaagaatat cgcaccacag gttattgaac
     1141 agcgcttgct gatacatccc tttattgagc aggttgccat cgttggggat gcacgaaaat
     1201 acatcgttgc cctgattgtg ccaaattttg aaacattaac acaatgggca gccactcaag
     1261 gaatatctgc accatccaat gacgaactgg ttaaacatcc tgatgtacgt aaaaaatatg
     1321 aggcaatagt taatgaggtt aatcaggagt ttggcaaggt tgaacagatt aaaaagttta
     1381 cactgcttcc acagcaattt acccaggaaa caggtgaatt aacgcctaca ctcaaaatca
     1441 aacgcaaagt tatccagaaa aaatatgcag atattataga agaaatgtac aaagaataaa
     1501 tttactcatg cggagagcgc tcataacaca gagtttagga tatatgctct gaccccattt
     1561 ccagcgctct ccgcaacctc cacataatac attttttgaa cgtatcccat tttttaaaaa
     1621 aaaattatca tcatatcttg acgaaaatca aatttatggc tatattgaca agtacgaacg
     1681 ttcgttcagg ggtgaattat gagccataca atcattgaca agcggcagca gattcttgat
     1741 gtggcaaaag agctttttgc cacaaaaggg tattcggcca cgggcatacg tgaaatagcc
     1801 gaaaaggcag ggctgtcgct ggggaatttc tataattact tcaaaaacaa agaagatcta
     1861 tttaaaagcc tgcttgaccc gcaaaatatc cttgagcatt taactgaaat caagctgatg
     1921 ttgaatgatg gctttccctt taatttcaaa gatataataa aatccataaa aacagtagtt
     1981 gatagcgact ttcacctgta caggcttata ttcattgacc tgaccgagtt taatggaatg
     2041 catactgata aaatacttga tagaataatc accgaagcaa ctgcaacatt taacgccaac
     2101 gttaaaaact caattgttgg caccgtcata aaagatgagg attatcagtt ttacattaag
     2161 gcatttatca tttcaacatt gtcacttctg gtaataggaa atattttgcc atcagcaaat
     2221 ttaaacgaca aatctaatga ctatattgca gaaaagcttt ccaatgtcat attgcatggg
     2281 attttgctat gaactattgg acacacatat ttttttgggc gatagttact attttagcaa
     2341 gtgggcttgc ctttgcacag gaacccttta gccttttatc cgtagaagac aaaccaaccc
     2401 tgagtatcaa gggatatgtt gaaattgaca ccaccacatc gacctacccc catcaggaaa
     2461 cagctgatat ctttaaaaaa gatgaactga aagcaaacac caccatcaaa tatggcaaag
     2521 agtcattcta tgtttatact gatataaatg cgtatctgtt gccaaacctg ataaacgaaa
     2581 acataaatcc tacatatcga tattctgaag aaaccgacat cctgcgaaat ggcaccataa
     2641 cgtccagcaa tgcagaaatt aatttccggg aatgctatat caactatgag ataggctctg
     2701 tacgtttgcg agcaggcaat caaatatttg catggggcac tgccgacgca tttaacccaa
     2761 catcgtactt taatacatac gaccttcgtg aatttctgtt caaggatgag gacactgaac
     2821 tcaagatggg cgttccagcg gtgtcaatgc ttatgaccct cggcaacgat acacttgagc
     2881 ttgtcatcac accgctgcat attccactgc gcatgccaca agcaggttca ttctggcaac
     2941 ttacagtgaa agaaggccca ttcccagtaa ctatcgcaca acctgaagct ctgccttttg
     3001 aaattgcaaa tgctggcgtt ggtgcacgct actatcacaa tattgctggt tttgatgttc
     3061 atctgagtgc atagcacggc cccgatacta taccctatct tttgcccgta aaaaccatac
     3121 tgcagccaaa tgaacctgtt tcaattctgg tagagccacg ctacgctatt gtgaatatgg
     3181 caggcactgc tctatcaaag gcatataaag attttgtatt tcaggttgag gcggtgtatt
     3241 cgcccgataa aactggcgtt gtggatgtta atttgcaaaa cttgagcgct gcacagctac
     3301 aaacgcttct tccctacaaa acgcgcaaaa gccattttgt aacatattca gcaggattca
     3361 attattttat acccatccgt gattatttta aagagcacga aggacaatgc gtgtttacct
     3421 ttgagtggag ccagtcgcac ttttttgata acaagctcat gaagccaatg ctttcggata
     3481 ttgttgtgct gagattacag gacagctttt ttaatgataa gcttgatcta aaaataagct
     3541 catttgttga aaagcaaacg agtagttaca tgataatgcc ccgcgtcagc taccgctttg
     3601 acagtggtct tacctttgat gtaacgtacg tatatctgca catgacagct gattcgtatt
     3661 ttgcagggta caataacaat gattgcgtaa cagggagggt tagctatgaa ttttaaaata
     3721 ctctttgtgg cgatagttac tgttatagcc tttac
//
LOCUS       DAOSZP010000169         3746 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_19766_length_3746_cov_3.471688, whole genome shotgun sequence.
ACCESSION   DAOSZP010000169 DAOSZP010000000
VERSION     DAOSZP010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3746
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19766_length_3746_cov_3.471688"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..616
                     /locus_tag="PK348_04780"
     CDS             <1..616
                     /locus_tag="PK348_04780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65560.1"
                     /translation="IKNKEIEKEYELGHKVLKQFLPPDKGIIHKTQYMMIQNPDSTIT
                     CYFNIYNPDPMTSYIALCPLQANISLLSLLIPAISVLMQSYSRLGFLPDHIIKKINGV
                     LKEKELIEENLPMMIVKKKKKQYCISRSGNCNMKVYVQKKTKVMPLNLQDAINQVNNI
                     KKLIITSGWLPNNIGGIDEVDETIDVDIHSIPENNFMLILSEIL"
     gene            613..2925
                     /locus_tag="PK348_04785"
     CDS             613..2925
                     /locus_tag="PK348_04785"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HXK65561.1"
                     /translation="MKKRILFYSFSIIYTLIFVLTVPYGYKNAVTYPFDRLLVGSTFL
                     YRLHDTPHALFKATLETCDSRTLNNDFQTLTDWECISTINNTTVKQHFKSNNKVALWL
                     VLILAFSSILLIVWAAGVYYARKDMFANLFILIANAVALNCLVYIDLFFVHRLHAVYP
                     AVSSLVPLCLFYIVFTMKESNQLVKTVCYVIVLVTGIVLSGTVYCNVLAFEKVYQLNG
                     IISVVLFIIAAIMLVCEHNHARFLIKRNIVVAGAIVAGGVLPFAVLLAGTYHDMQPST
                     LFSLLFTIAIPVSIGNGLLANYSLGSVANLQKNSISMVVDIIIAVVISTGLFYMYNFY
                     SILLSPPIIIGLFLAIMLIILHIRMLIIRALDDIIFLKNDEYSLSLQRIAEIITWPKN
                     LKEKLDAIENELKTLIKVKKISYAIIDNYQYNDERIIVLDEDSNLASFFKKRNETITS
                     ASFFSSRNDEKAVEEYIITHGIEACIPVCINESLKGILFVHNKGDYFTNVELNFLSML
                     ALQVHQLVINSKALTEYINSRNYEKELDLASYIQVRLFPKKPPQDCGIQFSIYSRPYL
                     KVTGDYYDIIPVDKNRVAVVIADISGHGLSAAMILSATSAIINGMLKEKKGIDKVVAE
                     LNHFLTIRYTGFELITLFIGLYNKRTRSMEYINAGHIAPLVVSKDKKLYHLEGRSKIL
                     GVDPMAQYYSSRYSFSAGDQMILYTDGLTDLYNSNSDITFGEDSLNEILNQSLTKPIE
                     EKIHAITEGIVTFGQEYIKDDITVIGMHFD"
     gene            2918..>3746
                     /locus_tag="PK348_04790"
     CDS             2918..>3746
                     /locus_tag="PK348_04790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65562.1"
                     /translation="MINAIITGNAFPDVKQQAPDVLMPGATVSMPGIPSYSYINPVFA
                     DINEAFSLQYRYAHINSGDIGTHYAATTLYGFTIAYSTVEALFAEKEVIQTQGESWYF
                     AKGIMLTDWLGVGVSYNRYTVNNDPVTSWGYSLLLMPSRFISFGMVIDHANKPSLDEV
                     TLKQREWYSIGIRPFGNTLSFAWDIKRYEGEPWKDVLHVFTLSGMVWRDIICSLQYDM
                     DRNISFALAIPFDISSRTGGSSLYDVGYMHFDKQKATAYTFGITLTEERFRNALSSKM
                     "
BASE COUNT         1170 a          625 c          738 g         1213 t
ORIGIN      
        1 gatcaaaaac aaagagattg agaaggaata tgaactggga cataaggtgt taaagcaatt
       61 tttaccccct gataaaggta ttattcacaa gactcaatat atgatgatac aaaatccgga
      121 cagtaccatt acctgttatt tcaatattta caatccggat ccaatgacct cctatattgc
      181 actatgtccg ttacaggcca atatttcact gttatcattg ttgattccgg caatcagtgt
      241 gttaatgcag agttattccc gtcttggatt tttacctgat catattataa agaaaataaa
      301 tggtgttctt aaagaaaaag aactcattga agaaaatttg cccatgatga ttgtgaagaa
      361 gaaaaagaaa caatattgca taagcaggtc cggaaactgt aatatgaagg tatacgttca
      421 gaaaaaaact aaagttatgc ccttgaacct gcaagatgct attaaccagg ttaataatat
      481 caaaaagctt ataataacat caggatggct tcccaataat attggaggaa tagatgaagt
      541 tgatgaaacc attgatgtgg acatccacag tataccagag aacaatttta tgttaatact
      601 ttcagaaata ctatgaaaaa acggatactt ttttacagtt tcagtattat atatactctg
      661 atctttgtgc tgactgttcc ttatggctat aagaatgcag ttacataccc atttgaccgg
      721 ctgctggtag gtagtacgtt tttgtatcgt ttgcatgata caccacatgc tttatttaaa
      781 gctacacttg aaacatgtga tagtagaact ctgaataatg atttccaaac gctaactgat
      841 tgggaatgta tatctaccat taataataca actgttaaac aacattttaa aagtaataat
      901 aaggtagcat tgtggctggt tttaatacta gcattttctt caattctttt gattgtatgg
      961 gctgcgggcg tctattatgc acggaaggac atgtttgcta atttatttat attgattgct
     1021 aatgctgtgg ctcttaattg tcttgtatac atagaccttt tttttgtgca tcggttacat
     1081 gctgtctatc ctgctgtcag ctctcttgtt cccctctgtt tattctatat tgtatttacg
     1141 atgaaagaaa gcaatcagct agttaaaaca gtgtgctatg tgattgttct tgtaaccggc
     1201 attgttttaa gtggaaccgt ctattgtaat gttttggcct ttgaaaaagt gtatcagctc
     1261 aatgggatta tttcagttgt attgtttatt atagctgcta tcatgttggt ttgtgagcac
     1321 aaccatgcac gatttttaat taaaagaaat attgtagtgg caggcgcaat agtagcaggt
     1381 ggtgttttgc catttgcagt gttgcttgca ggtacgtatc atgatatgca gccttctact
     1441 ttattttcac tcctgtttac catagctata ccggtgagta tagggaatgg gcttctggca
     1501 aattacagcc tggggtcggt ggccaatctt caaaaaaaca gtatatcaat ggttgtggat
     1561 attattatcg cagtggttat atcgacgggg ttattttata tgtataattt ttattccata
     1621 ttactatcgc ctcccattat tattggactt tttcttgcaa tcatgctgat tattctccac
     1681 atcaggatgc tcatcatacg ggcgcttgat gatattatat ttttaaaaaa cgatgaatat
     1741 tctctgtcat tacagagaat tgcagaaatc ataacctggc caaaaaattt aaaggaaaaa
     1801 ctggatgcta ttgaaaatga actgaagacg ctaatcaaag ttaaaaaaat aagctatgca
     1861 attattgata actatcagta taatgatgaa cgaattattg ttctggatga agattctaat
     1921 cttgcctcgt tttttaaaaa aaggaatgaa accattacct cggcaagttt tttttccagc
     1981 agaaatgatg aaaaggctgt tgaagaatac attataaccc acggtattga agcctgcatt
     2041 cccgtatgta taaatgaatc cttaaagggt attctttttg tccataacaa aggcgattat
     2101 tttacaaatg tagaactaaa ctttctttcc atgttagcct tgcaggttca tcagcttgtc
     2161 atcaacagca aggctttaac ggaatatatc aattcacgaa attatgagaa ggaacttgat
     2221 ctggcatcat atattcaggt tcgacttttc ccgaagaaac caccacagga ttgtggtatt
     2281 cagttttcta tatattcacg gccgtatttg aaggtaaccg gtgactacta tgatatcatc
     2341 cctgtggaca aaaacagggt ggcagtagtt attgccgaca tatcaggcca tgggttatct
     2401 gctgcaatga tattatcagc caccagcgct attatcaacg ggatgttaaa agaaaagaaa
     2461 ggtattgata aagtagttgc tgaattaaat cattttttaa ctatccgcta tacgggattt
     2521 gaactaataa cgttatttat aggcctgtat aataagcgga cacgatctat ggaatacatc
     2581 aatgcagggc acattgcacc actggtagtt tcaaaggata aaaaattata tcatctggaa
     2641 ggaagatcca aaatactggg tgttgaccca atggcacagt attattcttc gcgctattca
     2701 ttcagcgcag gtgatcagat gatactgtat acagatggat tgacagatct gtataacagt
     2761 aatagtgata taacatttgg tgaggattca ttgaatgaaa tactcaatca gtcattaaca
     2821 aaacctattg aggagaaaat ccatgcaatt acagaaggga ttgttacatt tggccaggaa
     2881 tatatcaagg atgacattac tgttattggc atgcattttg attaatgcta taattaccgg
     2941 gaatgccttt cctgatgtaa agcagcaagc accagatgta ttgatgccag gtgctacggt
     3001 atcaatgcca gggataccat cgtatagtta tataaatcca gtttttgctg atattaatga
     3061 agccttttca ttacagtacc gttatgcaca cattaattcc ggtgatatag gaacgcatta
     3121 tgccgctaca acattatacg gatttaccat tgcatatagt acagtggaag cattatttgc
     3181 tgaaaaagaa gttatacaaa cacaggggga atcatggtat tttgccaaag gtattatgct
     3241 cactgactgg ttgggtgttg gagtaagtta taaccggtat acagtgaaca atgaccctgt
     3301 aacatcatgg ggatacagtt tgcttttgat gccatcacgg ttcatttcat ttggtatggt
     3361 cattgatcat gctaataaac catcgttaga tgaggttaca ttaaaacagc gtgaatggta
     3421 ttctattggg attcgtccct ttggtaatac tctttcattt gcatgggaca ttaaacgcta
     3481 tgagggtgaa ccgtggaaag atgtattgca tgtgtttacc ctttcaggaa tggtgtggcg
     3541 cgatatcata tgctctttgc agtatgacat ggatcgcaat atatcatttg cactggctat
     3601 cccatttgac atatcatcac gaactggtgg tagttctctc tatgatgttg ggtatatgca
     3661 ttttgataag cagaaggcaa cagcctatac gtttggtatc acactaactg aagagcgctt
     3721 caggaatgca ttgagttcca agatgc
//
LOCUS       DAOSZP010000170         3738 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_19808_length_3738_cov_2.565029, whole genome shotgun sequence.
ACCESSION   DAOSZP010000170 DAOSZP010000000
VERSION     DAOSZP010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3738
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19808_length_3738_cov_2.565029"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..526)
                     /locus_tag="PK348_04795"
     CDS             complement(<1..526)
                     /locus_tag="PK348_04795"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HXK65563.1"
                     /translation="MRFEWMGRQESQYVADTIGQVYIELSTACNLSCATCVRHSIKDF
                     KNTHFTKKLLNRLVSQLQELSNLNRIVLLGFGEALCNPDIQWHLRQLSTLNVPIVLVT
                     NGLLITQHVAELFVSLPLEAVYISIDDPAGVKPTIRKGSDVHIPLQAITLLKETKHNI
                     KAQKPIVGVETVVTQ"
     gene            complement(550..1593)
                     /gene="arsB"
                     /locus_tag="PK348_04800"
     CDS             complement(550..1593)
                     /gene="arsB"
                     /locus_tag="PK348_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013876915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HXK65564.1"
                     /translation="MADSVAKKLSFVDRYLTLWIFLAIALGVAIGYYLPGVKGFINTL
                     QVGTTNIPIAIGLILMMYPPLAKVKYEEMGKIFQNKKLLVVSLLMNWVIGPIVMFVLA
                     VLLLRSYPEYMIGVILVGLARCIAMVIVWNELAGGDRELAVGMVAFNAVFQVLFYAVY
                     IYLFITLALNWFGLVKGLNISVSMSESAKTVFIYLGIPFLGGVITRFGLIALKGKQWY
                     ETQFIPKISPITLIALLFTIIVMFSMQGNKIIERPQDVVIAAFPMLIYFVLMWFVTFY
                     IVRRITGDYAKTTAVAFTAGSNDFELAIAVAIAIFGINSGQAFATVIGPLVEVPVLIG
                     LVNVALAFKKRYF"
     gene            complement(1665..2534)
                     /locus_tag="PK348_04805"
     CDS             complement(1665..2534)
                     /locus_tag="PK348_04805"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HXK65565.1"
                     /translation="MFSKVLFASDLSEASTHVIECIKDLKVYGTKEIILFHALGIKYL
                     EDLRYSLVKEAEPVLQKQKAMLERYGYSVTVHIANDGVSYELWKFAKQENVSMVVIGT
                     HGRSLAFDVSLGGTAHKIIHDISKPLLIVRLRSDDKAMPVCQAACIDIDKPLLFTTDF
                     SDTAELAFTYVEKMAESGIKHVVLMHVQDKTRIEKYLKDKLNEFNTIDTERLEMLKDR
                     LVKKGVKNVDCILKYGIPVEEIINESRNRDYSLIIMGALGRSLVKDVFIGSVSWNVVR
                     NASQPVLLIPPLK"
     gene            complement(2630..2839)
                     /locus_tag="PK348_04810"
     CDS             complement(2630..2839)
                     /locus_tag="PK348_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015050848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HXK65566.1"
                     /translation="MPAYEYRCDDCNETFTVIASMSEKEKGLQVVCKICGSTNVRQVY
                     SSISVGAKNKDLPIIGGGCSCGGAC"
     gene            complement(2884..3231)
                     /locus_tag="PK348_04815"
     CDS             complement(2884..3231)
                     /locus_tag="PK348_04815"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HXK65567.1"
                     /translation="MITQVKVSTIFKMLSDTNRLRIINLLMQKELCVCHMAELLGITQ
                     TNVSHHLAKLHAAGVVHYRKDGQWIYYSLSEDFVKDNHQLFEYLTQVFSLESIYKDDI
                     KTLHTMGDSHCRA"
     gene            complement(3331..>3738)
                     /locus_tag="PK348_04820"
     CDS             complement(3331..>3738)
                     /locus_tag="PK348_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HXK65568.1"
                     /translation="VACIFFIIGLHLLEFINIPFLQKSANPAISKKGLWAAFLLGLLF
                     GIALGPCTFAYMAPMLGVAFQVASTKLLFAIALVIMYAIGHCSVIVIAGTSYEVVQRY
                     LNWNERSKGALIVKKACGVLVIAGGVYLIISVL"
BASE COUNT         1227 a          811 c          563 g         1137 t
ORIGIN      
        1 gctgtgtaac aacagtttcc acacctacta tcggcttttg tgcttttatg ttgtgttttg
       61 tttctttaag caatgttatt gcctgcaatg gtatatgcac atcactacct ttgcggatag
      121 tgggtttaac tccagcagga tcatcaatgg atatatagac tgcttcaaga ggcaaagaaa
      181 caaataattc agctacatgc tgtgtaataa gcaaaccatt ggtaacaagt actatcggca
      241 cattcagtgt actcaattgt cgcagatgcc attgtatatc gggattacaa agagcctcac
      301 caaatcccaa taaaacaata cggttaaggt tcgatagttc ctgtaattga ctcaccagcc
      361 tgttaagtag ctttttagta aaatgggtat ttttaaaatc tttaatggaa tgacgtacac
      421 aggtggcaca cgataaatta caggcagtgg aaagctcaat atatacctga ccaatagtat
      481 cagctacata ttgactttcc tgcctgccca tccattcaaa acgcatatgt gtatctactg
      541 aaacaagctc taaaaatatc gttttttaaa tgccagagcc acgttgacaa ggccaatcaa
      601 cacgggtact tcaacaagcg gaccaataac cgttgcaaat gcctggccgg aattaatacc
      661 aaatatagca atggcaactg ctattgccag ttcaaaatca ttggaaccgg ctgtgaatgc
      721 cacagcagtt gtttttgcat aatcgccggt aatacgccgc acaatataga atgtgacaaa
      781 ccacattaac acaaagtata taagcattgg gaacgctgct ataacaacat cctgtgggcg
      841 ttcaataatc ttatttcctt gcattgaaaa catgacaata attgtaaaga gcagtgcaat
      901 taatgtaatg gggcttattt taggaataaa ttgagtttca taccactgct tccctttgag
      961 tgcaatcaaa ccaaagcgtg tgattacacc accaagaaat ggtatgccta aatatatgaa
     1021 taccgtttta gctgactcag acatagaaac tgagatattc agacccttaa ccagaccaaa
     1081 ccagttaagc gccagagtta taaacaaata gatatacact gcataaaata aaacctgaaa
     1141 taccgcatta aacgcaacca taccaacagc aagttcacga tcaccgccag cgagctcatt
     1201 ccacacaatt accattgcaa tacaccgtgc aagcccaacc agtataacac caatcatata
     1261 ttcaggatat gagcgcaaaa gcagaactgc cagtacaaac atgacaattg ggccaatcac
     1321 ccagttcatc agcaatgaaa ctaccagcaa ttttttattc tgaaagattt ttcccatttc
     1381 ctcatattta accttagcaa gaggcggata catcatgagg ataagcccta tggcaatggg
     1441 aatattggta gtcccaacct gcaacgtatt aatgaagcct tttacgccag gcaaataata
     1501 ccctatagca acacccagtg ctattgctaa aaatatccat aatgttaaat accggtcaac
     1561 aaatgaaagc ttttttgcta cactatcagc catagttata cctcttttat gtaataatta
     1621 tttcactcag tgataattca cattacttag tattactgat tgttttattt taaaggtggt
     1681 attaacaata ccggttgaga agcattacgt actacattcc agcttacact accaataaac
     1741 acatccttca caaggcttct acctaacgct cccataatta tcaatgaata atccctgttt
     1801 ctggactcat tgataatctc ttctaccggt attccatatt ttaaaataca atcaacgttt
     1861 tttacacctt ttttcaccag cctgtctttc aacatttcaa gcctttcagt atcaattgta
     1921 ttaaattcat taagtttatc ttttaaatat ttttcaatcc ttgttttatc ctgtacatgc
     1981 atgagcacta catgtttaat accactttct gccatctttt caacataggt aaatgcaagt
     2041 tcagcagtat ccgaaaaatc tgttgtaaaa agcaagggct tatcaatatc aatacatgcg
     2101 gcctgacata ccggcattgc tttatcatca gaacgcaaac gtacaataag aagtggtttt
     2161 gatatatcat gtattatttt atgtgcagtg ccacctaacg atacatcaaa cgccaaactg
     2221 cgcccatgcg ttccgatgac aaccattgaa acattttctt gcttagcaaa tttccacaat
     2281 tcatatgata caccatcatt tgcaatatgc actgttaccg aatacccata gcgttcaagc
     2341 attgcttttt gtttttgtag taccggttca gcttctttta ccagagaata ccgtaaatct
     2401 tctagatatt taattcctaa tgcgtgaaat aaaattattt ctttagttcc atacactttt
     2461 aaatctttaa tgcattcaat gacatgagtg gatgcttctg acagatcaga agcaaatagt
     2521 actttgctaa acattgctgc ctccagataa attttaaatt ttaactacag tgtaattcaa
     2581 aacacatgtt gtggttatta attcttttca cagtaattta tttgcatgat caacatgctc
     2641 ctccacagga acaaccaccg ccaataatgg ggagatcctt attctttgca ccaacagaaa
     2701 tacttgaata tacctgccgt acattggtgc ttccacatat tttgcatacc acctgcaacc
     2761 ctttttcctt ttcactcata gaggcaatga cagtaaatgt ttcattacag tcatcacaac
     2821 gatattcata cgctggcatt gaaataattc ctccttattt aaaaaaataa taccttttaa
     2881 actttatgcc ctacaatggg aatcacccat tgtatgcagt gtttttatat catccttata
     2941 tattgattcc aatgaaaaca cctgtgtaag atactcaaat aattgatgat tatccttaac
     3001 aaaatcttca ctaaggctat aatatatcca ctgcccatct ttccgatagt gtaccacgcc
     3061 ggctgcatgg agctttgcta agtgatgaga tacgttggtt tgcgttattc caagcaactc
     3121 agccatatgg cacacacata attctttctg catgagtaga tttattattc ttagcctgtt
     3181 agtatcagaa agcattttaa atatggttga tactttaacc tgtgttatca tactgatagt
     3241 ccatgttgcc tgtatatatc aatatatgaa caaatattca tatatcaaac ataccgcata
     3301 ttacttttaa atcaattttt tttccttaca ttataaaaca ctaataatta aatatactcc
     3361 accagcaata accagcactc cacatgcttt tttaacaatc aaggcacctt ttgaacgttc
     3421 attccagtta aggtaacgct gcaccacttc atacgatgtt cctgcaataa ctattacact
     3481 gcaatgacca attgcataca tgattaccag agctatcgca aataaaagct ttgttgatgc
     3541 tacctgaaac gcaacgccca gcattggtgc catataggca aaagtgcagg gccccagcgc
     3601 aatgccaaac aaaagtccta acaaaaaggc agcccacaag ccttttttac taattgctgg
     3661 atttgcgctt ttttgtaaaa atggtatatt aataaactcc agtaaatgca acccaatgat
     3721 aaaaaaaata catgccac
//
LOCUS       DAOSZP010000171         3696 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_20092_length_3696_cov_3.148586, whole genome shotgun sequence.
ACCESSION   DAOSZP010000171 DAOSZP010000000
VERSION     DAOSZP010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3696
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20092_length_3696_cov_3.148586"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            460..672
                     /locus_tag="PK348_04825"
     CDS             460..672
                     /locus_tag="PK348_04825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65569.1"
                     /translation="MLSPRTVSRREMIRLSATTLLSLSPLSTISCGRRLIPVDTDIDP
                     DIYEEKNTVKAAEVISIKEKKTCLIS"
     gene            657..1601
                     /locus_tag="PK348_04830"
     CDS             657..1601
                     /locus_tag="PK348_04830"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HXK65570.1"
                     /translation="MPNIIIILTDDMGYGDIGCYGNKAINTPHIDRMAREGMRFIDFY
                     CASPLCSPSRAGLLTGRYPLRCGITFPLQPGKDTFMRKIVKELGYMFGALGVVDLHGA
                     KNLVNGLPHSEITIAQALKLRNYATCAIGKWHLGDFVVDETYHPHNYGFDKFVGFNAS
                     NDDWPVSYFKDKKMIIEDIGLEQQPYTGLFTQEAIQFINENHKKPFFLYLAHKDPHQP
                     CFPSKKFEGTTKAGPHGDTVAEVDWSVGQILQCLRKNNIDSKTLVIFTSDNGPWFDGS
                     TGGLRGRKGESYDGVFRVPMIAWWPGTIKKGQCAINQL"
     gene            1598..>1845
                     /locus_tag="PK348_04835"
     CDS             1598..>1845
                     /locus_tag="PK348_04835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65571.1"
                     /translation="MNIDFFPTVCNLAGLTLPKDRIIDGKDLMPLLTGKSKQSPHDAL
                     YFFHYREIEGIRSGRWKYIRYNNTRGWPVPLDKPDTFFG"
     assembly_gap    1846..1855
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1856..2052
                     /locus_tag="PK348_04840"
     CDS             <1856..2052
                     /locus_tag="PK348_04840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65572.1"
                     /translation="HDCEPPGSNQKVPTLATWPVLYDMEYDKSESYNVIKRYPAIGKK
                     LHEKLLAFEKEFYANIRGWK"
     gene            2064..3278
                     /locus_tag="PK348_04845"
     CDS             2064..3278
                     /locus_tag="PK348_04845"
                     /inference="COORDINATES: protein motif:HMM:TIGR03942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HXK65573.1"
                     /translation="MGSLSFLVKPVSYHCNVACNYCFYKRVVDLYPGSLQIMPFDVAE
                     TVIKAAIGTGSTYISFCWQGGEPTLAGLDFFREIVTVQNRLKTDVQIIENSLQTNGIL
                     LNDEWCEFLSRNNVLVGISLDGPEVIHDTYRRDYAGHGTFEKVMHAIALMNEYAVQYN
                     ILTLVTNANVTHARELYAFFRHNNFAYLQFIPCFEHDEKGNIMPYSIRGKDLGNFYCE
                     LFDAWYQDGFPYVSVRLFQDLLMYSLGAKTSCCWLGECNSYIVVEHNGDCYPCDFFVY
                     PEWHLGNIMNYRLQDILNNEKRNTFAQLKRETTPECRMCSINNFCMGDCTRYRFTTDI
                     HQRGTSKLCEAWKMLFEHYQHYEKELAERVSKLQKSVQTGKFFQLQRNDICPCGSGKK
                     FKKCCGRAPLAI"
BASE COUNT         1180 a          593 c          793 g         1120 t
ORIGIN      
        1 aattatttac ttctattggc tattacatct ataacctttg taagtaatga ttttaattat
       61 atgtgggata ttgataaaat gtgggaacat ggtacttttt gaattttcta ctaattgaat
      121 atctaaggtc attgtaaatc tcccatgcaa aaaagaattg atgaagtgta atggtcgtgc
      181 atgaataaag caacaaaaat aaagaaaaaa cctcataaat ttgcttgcaa ttacgttgtg
      241 tgagtatatg ctttaaatgc ttttgtatat ttggaggaat gataaaaaaa gtcaaatagt
      301 tgcgataata gcatgaactg ttcataatgg tggttaaggt gtgccacatg gacaaaaaag
      361 gtattattcg tcatgttaca tggtctctga ggcaggtttg gatagtgagg aattataacc
      421 gcaacattca atgatcttag taatcaaaga ggaggttcta tgctaagtcc acgtactgta
      481 tcacgccgtg agatgatccg attatctgct acaacgttac tttcattatc gccattaagt
      541 actattagct gtggcaggcg cctgatacct gttgatacag atattgaccc agatatatat
      601 gaggaaaaga acactgttaa agctgctgaa gttatcagta taaaagaaaa aaaaacgtgc
      661 ctaatatcat aataatactt actgatgata tggggtatgg tgatattggt tgttatggca
      721 ataaagcaat taacactccg catattgaca gaatggcgcg tgaaggtatg cgatttatcg
      781 atttttattg tgcaagtcct ctttgttcac cttcaagggc ggggctttta accggccgtt
      841 acccattgcg gtgtggcatt acattcccat tgcaaccggg aaaagatacc tttatgcgca
      901 aaattgtaaa agagttgggt tatatgtttg gtgctcttgg tgtggtggat ttacatggtg
      961 caaaaaattt ggtcaatggt ttgcctcatt ctgaaataac tatcgcccag gctttaaaat
     1021 tgcgtaacta tgcaacctgt gccataggca aatggcattt aggtgatttt gttgtggatg
     1081 aaacgtatca tcctcacaat tatggttttg acaaatttgt gggtttcaat gcaagtaatg
     1141 atgactggcc agtttcatat tttaaagata aaaaaatgat catagaggat attgggcttg
     1201 aacagcaacc atacacaggg ctatttacac aggaagcaat acaatttata aatgaaaatc
     1261 ataaaaaacc tttctttctt tatttagcac ataaagatcc gcatcaacca tgcttcccgt
     1321 caaaaaagtt tgaaggaaca acaaaagctg gccctcatgg tgataccgtt gcagaagttg
     1381 attggagtgt gggccagata ctacaatgtc taagaaaaaa taatattgat tcaaaaacgc
     1441 tggttatatt taccagcgat aacgggccgt ggtttgatgg tagtactggc ggattgcgtg
     1501 gaaggaaagg ggagagctat gatggtgtat tcagggttcc aatgattgca tggtggcctg
     1561 gaactataaa gaaaggacag tgtgcaatca accagttatg aatattgatt ttttccctac
     1621 agtatgcaat ctggcaggat tgacattacc caaagatcgt attatagatg ggaaggattt
     1681 aatgcctctg ttaacaggca aatcaaagca atcacctcat gatgcattat atttttttca
     1741 ttacagagaa atagaaggaa tacgttcagg aaggtggaaa tatatacgat acaacaatac
     1801 acggggatgg cctgtacctc tggataaacc tgatacattt tttggnnnnn nnnnngtcat
     1861 gattgcgaac cacctggttc caatcaaaaa gtcccaacac tggctacatg gcctgttctt
     1921 tatgacatgg aatatgataa aagtgagagc tacaatgtta taaaacgcta tccagctatt
     1981 gggaaaaaac ttcatgaaaa acttttggct tttgaaaagg aattctatgc aaatatacgt
     2041 ggctggaaat aactgttact aatatgggaa gcctttcatt cttagtaaaa ccggtatcat
     2101 atcattgtaa tgtagcgtgc aattactgtt tttacaaacg ggtagttgat ctatatccgg
     2161 ggagtcttca gataatgccg tttgatgtag ctgagacggt aataaaagca gcaattggta
     2221 ctggctcaac gtatatttca ttctgctggc agggtggtga accaacgtta gcagggcttg
     2281 atttttttag ggagatagtt actgttcaaa accgtttaaa gacagatgtt caaatcatag
     2341 aaaattcact tcaaacaaat ggaattcttc tcaatgatga atggtgtgaa tttttatcac
     2401 gtaacaatgt tctggttggt ataagcctgg atgggccaga ggtaatccat gatacctatc
     2461 gaagagatta tgcaggccat ggtacttttg aaaaagttat gcatgcgata gctcttatga
     2521 atgaatatgc cgtgcagtat aatatcctta cattagtaac caatgcaaat gttacacatg
     2581 cacgtgaact ctatgccttc ttccgccaca ataattttgc ctatcttcag tttattccat
     2641 gttttgaaca tgatgaaaag ggaaatataa tgccatacag tatacgtgga aaggatctgg
     2701 gaaattttta ctgtgagctt tttgatgcat ggtatcagga tggttttccc tatgtttctg
     2761 ttcgtttgtt tcaggatctt ctcatgtatt ccttgggtgc aaaaacgtca tgttgctggc
     2821 ttggtgaatg caattcatat atcgttgttg agcacaatgg cgactgttac ccctgtgatt
     2881 tttttgtgta tccggagtgg cacttaggta atattatgaa ctatcgctta caggatattt
     2941 tgaataatga aaagcgaaat acatttgcgc agctaaaacg tgaaacaact ccagaatgca
     3001 ggatgtgcag cattaataat ttttgtatgg gcgattgtac caggtataga ttcactactg
     3061 atatacatca aagaggcaca agtaaattgt gtgaagcgtg gaagatgctt tttgagcatt
     3121 accagcacta tgaaaaggaa ttggcagaaa gggtcagtaa attacagaaa tcagtacaaa
     3181 caggaaagtt tttccagtta cagcgtaatg acatttgccc gtgtggtagt ggcaaaaaat
     3241 ttaaaaaatg ctgtggaagg gcacctcttg ccatataata taaaaatatt aaaatttaga
     3301 gaaaaaaaga gaaaataaaa tcatattttg caataaatac gattattccc tgataaaaag
     3361 caaaatacac aaaattttag cattcatgtt ttaggcctac ggtgacgaaa agtcgagcag
     3421 ggagtagtgt ctcctgacat tggtctttga aaacacctgt tggggtttgt ggtttgatag
     3481 tagcactcca ggcttggcct tttaatggtt ctggtcttgt atgagcccag atacaagcct
     3541 ggaacagggt agctttgtat ctactcaccc ttacactgca ctgtctgccc ggtgtggtgt
     3601 aattaatggc agggaagcca aaactaatgt gcaaaggagt gtaaaatgat tatcaaccac
     3661 aacatgagtg ctgtaaacgc taacagaata cttaag
//
LOCUS       DAOSZP010000172         3682 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_20174_length_3682_cov_2.732286, whole genome shotgun sequence.
ACCESSION   DAOSZP010000172 DAOSZP010000000
VERSION     DAOSZP010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3682
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20174_length_3682_cov_2.732286"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2751
                     /locus_tag="PK348_04850"
     CDS             <1..2751
                     /locus_tag="PK348_04850"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HXK65574.1"
                     /translation="EKVDLVKGILPQDVYKPIVTRFDPNSLPVMNIAITSQDMDLRQL
                     RFETEKNIVPLFERIDGVGNAKVTGGKVRQIEVLVNRDALQAYNTGIHEIIDSIQSSH
                     YNYPAGTLITGNKELLIRTAGEFKNIGDIENVPVKKTQDGRLVFLKHVARVIDGFKEI
                     TSQSFINGHPCVTLSIIKESGKNTVEVCCKTRNLVDNLQKKYRRRLSFDIISDQSQYI
                     RSAISSVVSSAIAGSFIAFFVLLLFTQRFYSALIITIAIPVSIIIAFFFMNIFSININ
                     MMSLGGLTICVGMLVDCGIVVIESITNEKNTSLPQAMYTIAPSLIASTLTSVVIFLPL
                     LLIKGLGGALFAHLAITVTIALLASLFVALLLIPALHTIHSQVAHHSKKQQHSAAIVH
                     SNVLFHKVDVLLHTIEHRYLLLLDTLLLQSKYIYSITFGIMVLGICAVIIMDRSLMPD
                     TTQSHCTIRLEASPGTPLQQTIDMLHYIDSLLERYPDIDKRLITAGYNPEDYTEYFGR
                     EKNTNIGQIHVQLHNGADARDCITYLENNVKLPSDICIEYNASNTDFLQVLRAGSGNI
                     TINCTGDSLEDISATADAFIRECTGQPWITQCSSEIKQGKPELTIQADTDKLASFGLT
                     LQDLASYIHTAVYGTHSGKYTESDYEVDIVTRFDHRFRDSIDDIGMIPIQAHGGAPCT
                     LLHTIASLSNSQGYSSIIRNNQQRCIPIHIETNSISQEKAIEQLQRLWEKQKKQNVSF
                     VLSQEMIETKESLQSLYAILVLSIVLIYMIIAAQFESLLYPLLIMSSVPVALCGSFIF
                     LFFTGKSINIMSLMGAVVLIGTIVNNAILLLDTIIHNYRQNGNVFASIAKSCTQRLRP
                     IIMTSLTTICGLIPLALGLQEGADVQSPLAIAIIGGMIAGTGFIMLCLPHFIRLAIKD
                     RP"
     gene            2748..>3682
                     /locus_tag="PK348_04855"
     CDS             2748..>3682
                     /locus_tag="PK348_04855"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HXK65575.1"
                     /translation="MKLIHHFVTHTITTLMLFCAIMATGFFAFTSLPSSLLPDAGNHD
                     ITIVVRYPGQSPYKIEEIIVNPLEDAISTIGGIEEIVSSSNDTEARIYITFEKDANIQ
                     FKVYEIHEKIEPVRAMFPDDVNEPEVYLQGNDYSPIMVIGLSSTQHSLNKLRDIAEGI
                     YKKNIERIEGVSQIEIGGGNKREIHVTVDNNYCVAHNIPLQRITEQIQKNNVITSIGI
                     LHDTMKDYALHLKSRFFTLDEIKHLPLLSGSGCLLTRLGKIAHVTDYAGIQDSLSRYN
                     TLDQVSLYIYKTSTANPLDVSQHIISILSQCSIPGV"
BASE COUNT         1175 a          744 c          653 g         1110 t
ORIGIN      
        1 gaaaaagttg atcttgtaaa aggtatcctc cctcaggatg tctataagcc aattgttaca
       61 cgctttgatc ctaattcttt accagttatg aacatagcta ttacatcaca ggatatggat
      121 ttacgacagc tgcgctttga aacagaaaaa aatatagtac cactttttga acgtattgat
      181 ggagttggca atgctaaagt aacaggaggc aaagtacgcc agattgaagt acttgtaaac
      241 cgtgatgcac tgcaggcata caatactgga atacatgaga taatcgatag catacagtca
      301 tctcactaca attatcctgc aggtacactc attacaggaa ataaagaact ccttatacga
      361 actgcaggtg agtttaaaaa cattggtgac attgaaaatg ttcctgttaa gaaaacccag
      421 gatggccgct tagtcttttt aaaacatgtt gcccgcgtta tcgatggctt caaagaaatc
      481 accagccagt catttatcaa tggtcatccc tgtgttaccc tatccattat taaagagtca
      541 ggcaaaaaca ctgttgaggt gtgctgcaag acacgcaacc ttgttgacaa cctgcaaaaa
      601 aaataccgca gaagattatc atttgatata atcagcgatc agtcgcaata catacgcagt
      661 gccatatcca gtgttgtgtc atctgctata gctggttctt tcattgcatt ttttgttctt
      721 ctccttttca cacagcgttt ctattcagca ctcattatta ccatagcaat acctgtatca
      781 attatcattg ccttcttttt catgaacata ttttccatta atatcaacat gatgtcatta
      841 ggcggattga ccatatgcgt gggcatgctt gttgactgtg gcatagtagt tattgaatcc
      901 attaccaatg aaaaaaatac atcactaccg caagccatgt ataccatcgc accatcactt
      961 attgcatcta cattaaccag tgtggtcatt tttctgccac tactactcat aaagggattg
     1021 ggcggcgccc tatttgcaca tttagcaatt acagtaacta tcgccctttt agcttctctt
     1081 tttgttgcat tgcttcttat ccctgcactg cacaccatcc attcacaagt agctcatcat
     1141 tctaaaaagc aacagcatag tgcagcgata gttcatagta atgtactttt ccacaaagta
     1201 gatgtattgc tgcacactat tgaacacaga taccttttgc tgcttgatac actactttta
     1261 cagagtaaat atatttactc aataactttc ggtattatgg tgctcggaat atgtgcagtt
     1321 attattatgg acaggtcact gatgcctgat acaacacaga gtcattgtac catcagattg
     1381 gaagcatcac ctggtacccc attgcagcaa acaattgata tgcttcacta tattgattca
     1441 ttgttggaac gctatcctga tattgacaaa cgattgataa cagcaggcta taatcccgaa
     1501 gattacaccg aatattttgg cagggaaaag aatactaata ttggccagat tcatgtacaa
     1561 ctgcacaatg gagcagatgc cagggattgc attacatatt tagagaacaa tgttaaactc
     1621 ccatctgata tttgcattga atataatgca tcaaatactg attttttaca ggtgttacgt
     1681 gcaggtagtg gtaatataac gattaattgt acaggcgatt ctttagaaga tattagtgcc
     1741 actgccgatg catttatacg tgaatgtact ggacaaccat ggatcacaca atgttccagt
     1801 gagattaaac agggcaagcc tgaacttacc atacaggctg atactgacaa gttagcatct
     1861 tttggattaa cacttcagga tcttgcttca tatatacaca ctgctgtgta tggaacacac
     1921 tcgggaaaat acactgaatc agattatgaa gttgatattg ttactcgatt tgatcacagg
     1981 ttccgtgaca gtatagatga cataggaatg atacccattc aggctcatgg tggtgctcca
     2041 tgtacattac ttcatactat tgcttcactt tcaaattcac agggatattc gtcaattata
     2101 cgaaacaatc agcagcgatg cattcccatt catattgaaa caaatagcat ttcacaggaa
     2161 aaagcaatag aacaactaca acggctgtgg gaaaagcaga aaaaacaaaa tgtttcgttt
     2221 gttttatcac aggaaatgat agaaacaaag gaatcacttc aatcgctata tgctatactt
     2281 gtactttcta ttgtgttaat atacatgatt atcgccgcac aatttgaatc acttctttac
     2341 ccactgttaa tcatgtcatc agtacctgtt gccttatgtg gatcattcat atttcttttt
     2401 ttcacaggaa aatcaataaa tatcatgtct ctcatgggag cagttgttct tataggtact
     2461 attgtaaata atgctatatt gcttcttgat actattattc ataactatcg ccaaaatggt
     2521 aatgtatttg catcaattgc aaaatcctgc acacagcgac tacgtccaat cataatgaca
     2581 tcacttacta ccatttgtgg gcttatacca ctggcattag gattacagga aggcgctgat
     2641 gttcagtcac cacttgctat tgctatcatt ggcggcatga ttgcaggaac aggctttatc
     2701 atgctctgcc tgccccattt catacgttta gcaattaagg acaggccatg aaattaatac
     2761 accattttgt aactcataca ataacaacgt tgatgctttt ttgtgcaatc atggcaacag
     2821 gcttttttgc atttacatca ttgccttcat cccttttacc cgatgcaggt aatcatgaca
     2881 taaccattgt ggtacggtat cctgggcaat ccccctataa aattgaggag ataattgtca
     2941 acccacttga agatgcaata agcactatag gcggaataga agagattgtt tcttcaagca
     3001 acgataccga agcacgcata tacataacct ttgaaaagga tgccaatata caatttaaag
     3061 tgtatgaaat acatgaaaag atagaacctg tacgcgcaat gtttccagac gatgttaatg
     3121 aacctgaggt atatcttcag ggcaatgact attcacctat aatggtcata gggttatcaa
     3181 gtacacagca tagccttaac aaactgcgtg atattgcaga aggcatatat aaaaaaaata
     3241 ttgaacgcat tgagggtgtt tctcaaattg agataggcgg aggcaataag cgtgagattc
     3301 atgtaaccgt tgataataat tactgtgttg ctcacaacat tccattgcaa cgcataacag
     3361 aacaaattca aaaaaacaat gttattactt ccatcgggat tctgcatgat acaatgaaag
     3421 attatgcatt gcaccttaaa agcagattct ttacactgga tgaaattaaa cacctgccac
     3481 tattatcagg atctggatgt ttgctaacgc gtttaggtaa aatagcgcat gtaacagact
     3541 atgcgggtat acaggattca ctatcacgat acaacactct ggatcaggtt tcattatata
     3601 tctataaaac aagtacagct aatcccttag atgtatcaca gcacattatc agcatactat
     3661 cacaatgctc tatacctgga gt
//
LOCUS       DAOSZP010000173         3655 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_20380_length_3655_cov_3.185278, whole genome shotgun sequence.
ACCESSION   DAOSZP010000173 DAOSZP010000000
VERSION     DAOSZP010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3655
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20380_length_3655_cov_3.185278"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1407
                     /locus_tag="PK348_04860"
     CDS             <1..1407
                     /locus_tag="PK348_04860"
                     /inference="COORDINATES: protein motif:HMM:NF012503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HXK65576.1"
                     /translation="PYYIGHMTSAIPYFMILLEMIIAALNQNQVKIETAKASTFVERE
                     FISWIHRLIFQKPASFYKRNIQNHRIALGNVTLDGTLANLTAMLVARNKAFPADEHFP
                     GIRKAGVAEAFRYYNIHRAVFIVSQRGHYSIDKVARITGIGDNNVIKIPVDSNNKIDI
                     NALRRELKAIAEYNSTHADTIKVIALIGIAGTTETGNIDDLYTLRKIADEYNIFFHVD
                     AAWGGSVLLVNKYRDLFKGIEKADSVTFDAHKLLYSPLSMGLILFKNPYDLTNIMHTS
                     NYIIRPDSVDQGRFTVEGSRPFSCLKPWVTLKIFGTSGFEILFDHAFMLTDTLKDLLI
                     KHEDFELLNNPELFITNYRFVPESVQTKIEHIYQRLQNANPQDTIRLKKRLAKINDVL
                     NEMTIELHRAIRQEDNSFVSRTMLESTRYRDQRIVVLRAITINPLTTPAILQEIIDEQ
                     NKLGLKIYNDFKNRLSRV"
     gene            1376..2377
                     /locus_tag="PK348_04865"
     CDS             1376..2377
                     /locus_tag="PK348_04865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65577.1"
                     /translation="MISKTGYHVYRIAFFSILFFLSFHFSFYSLAIGTAFPTLDNGTL
                     YTEPSVTVPVQGVVLDYAVRDTAILFVAKNEKEIVAGAYTIQDKNITQFPVTTHSENT
                     QIKRIAFESGAIYICIHESDSVLYRADVNTGMVEQVKGVSDFLILKDSLVILKSDNTL
                     VYNDFVLPLYFTGKPLLKGHVDDRIVFVTDDSDTEVIDIKEKKVVYRYSKNVQYVVSS
                     DYTVEVAINDLPTGEKSPRIFYKIYCNGQDYGRTEPVLAINQSKIQLNLAPEQYHEIV
                     IERWRLDENSEQYIRDNNIRQPKPVIVFVYPGRIIVVRLTYTGLEYNVFTGFKVMER"
     gene            complement(2451..3290)
                     /locus_tag="PK348_04870"
     CDS             complement(2451..3290)
                     /locus_tag="PK348_04870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65578.1"
                     /translation="MNSTRFLLYTIVAVLALSLSVTAIAQQQSPKEEQKKEGIKEEVT
                     GWRIKDFKPYVKAIQELEKLSKEYSDNLLKLAIDEYSTGIDILEDMENDVNKLIETNQ
                     KNKNLQEQWYWQEIDRREQEKRQIAMMKFEAKTKAVTYFTRAINHMDEITFEEVRKDP
                     KFVNFQIRLYQAYVSTQYDLYNLKPCIPVLERYIEINDQTKNDAWAYKYLSSCYGYME
                     GVLLKYKQGTEQEQLQYKQKKNMYMLRAAELQYGIDSPQYKHLREIVSKDEKKSEKIN
                     EFK"
BASE COUNT         1107 a          649 c          676 g         1223 t
ORIGIN      
        1 ccctattata tagggcacat gacaagtgct attccatatt ttatgatttt gcttgagatg
       61 atcattgctg cactcaatca aaatcaggta aagatagaaa cagcaaaagc gtcaacattt
      121 gtagaacgcg aatttatttc ctggatacac agacttattt ttcaaaagcc agcgtcgttt
      181 tataaacgaa atattcagaa tcacagaatt gcactgggaa atgtaacgct tgatggcaca
      241 ctggcaaacc ttacagcaat gctggtggca cgcaacaagg catttcctgc tgatgaacac
      301 tttcccggta tacgcaaagc aggtgtggcc gaagcattca ggtattataa tatacataga
      361 gcagtgttta ttgtatcgca gcgtggacat tattcaattg ataaggttgc ccgtatcacc
      421 ggtattggcg ataataacgt aatcaaaata cccgtggata gtaataataa aatagatatc
      481 aatgctttgc gacgtgagtt aaaagcaata gctgagtaca acagcaccca tgctgataca
      541 attaaagtta ttgctttgat tggcattgcc ggcacaactg agacaggcaa tattgatgac
      601 ctgtacacct tacgaaaaat tgctgatgaa tacaatattt tcttccacgt tgatgcggcg
      661 tggggtgggt cagtgcttct ggttaacaaa taccgcgatt tattcaaagg gatagaaaag
      721 gctgattcag ttacatttga tgctcataaa ctgttgtatt caccattgtc catgggactg
      781 attttgttta aaaatcccta tgaccttacc aacatcatgc atacatccaa ttacatcatt
      841 cgccctgatt cagttgatca gggaaggttt actgtggaag gttcgcgccc gttttcttgc
      901 ttaaagccct gggtaacgct gaagatattt ggcacatcag gttttgagat tttatttgat
      961 catgccttta tgctgactga tacattaaag gatcttttaa ttaagcatga agactttgag
     1021 ttactcaata atccggaatt atttataacc aattaccgct ttgttcctga gagtgtacag
     1081 acaaaaattg aacatattta ccaaagacta caaaatgcca atccacagga tactatacga
     1141 cttaaaaaac ggttagcaaa aattaatgat gttctaaacg aaatgactat tgagcttcac
     1201 cgtgctatcc gtcaggaaga taacagcttt gtttcacgaa ccatgctgga atcaacacgc
     1261 tatcgcgacc agcgaattgt agttctcagg gctatcacca taaatccttt aacaactccg
     1321 gcaatcttgc aagaaataat tgacgaacag aataagcttg gtttaaagat atataatgat
     1381 ttcaaaaacc ggttatcacg tgtataggat tgccttcttt agtatactat tctttttgtc
     1441 atttcatttt tctttttata gcctggcgat aggcactgca tttccaacat tagataatgg
     1501 aacattatat actgagcctt cagtgaccgt gcctgttcaa ggcgttgttc ttgattatgc
     1561 cgtcagggat actgcaattc tgtttgttgc aaaaaatgaa aaagaaatag ttgcgggagc
     1621 ttatactata caggataaaa acattactca atttcctgtt acaacgcatt ctgaaaacac
     1681 acaaatcaaa cgcatagctt ttgaatcagg tgccatatat atctgtattc atgaaagtga
     1741 tagtgtatta tacagggctg atgtgaatac aggcatggtg gagcaggtta agggggttag
     1801 cgatttttta atattgaaag atagcctggt tatccttaaa agcgataata cccttgtgta
     1861 caatgatttt gtattgccat tatattttac aggcaagccg ttattgaaag ggcatgtgga
     1921 tgatcgcata gtctttgtaa ccgatgacag tgatactgaa gttattgata ttaaagaaaa
     1981 gaaagtagta tatcgctata gtaaaaacgt acaatatgtt gtaagcagcg attatactgt
     2041 tgaggttgct atcaatgatc ttccaaccgg tgaaaaatcc cccaggattt tttataaaat
     2101 atactgtaat ggccaggatt atgggcgtac agagcctgta cttgcaatta atcaatctaa
     2161 gattcagctt aaccttgcac ctgagcagta tcatgaaatc gtgattgaac gctggcgtct
     2221 tgatgaaaat tcagaacagt atatacggga taataatatt cggcagccaa aaccagttat
     2281 agtatttgta taccctggcc gaatcatcgt agtcagactg acctatactg gattggaata
     2341 caatgtgttt acaggtttta aggtgatgga aagataaaac cccgtactgt aacggggttt
     2401 tttattgata gtgaacaatt cctgcttaaa gtagcgtgaa cgtaatattc ttatttgaac
     2461 tcgtttattt tttcactctt cttttcgtct tttgatacta tttcgcgcaa atgtttatac
     2521 tgaggtgaat caataccata ctgcagttcg gctgctctca gcatatacat gttctttttc
     2581 tgcttgtact gcaactgttc ctgttctgtt ccctgcttgt atttaagcaa cacaccttcc
     2641 atatacccat aacagcttga caggtactta tatgcccatg catcgttttt ggtctgatca
     2701 ttaatttcaa tatatcgttc cagaaccgga atacagggtt tcaagttata caggtcgtac
     2761 tgagttgaaa catatgcctg gtacaatcgt atctggaagt tgacaaactt ggggtctttt
     2821 cgtacttctt caaaggttat ttcatccata tgattgatag ctctggtaaa atatgtcact
     2881 gcttttgttt ttgcttcaaa tttcatcatg gcaatctggc gtttttcctg ttcacggcgg
     2941 tcaatttcct gccagtacca ctgttcctga aggtttttgt ttttctggtt ggtttcaatg
     3001 agcttattga catcattttc catatcttca agtatatcta taccagttga atattcatca
     3061 attgccagtt ttaaaagatt gtctgaatat tctttgctga gtttttccag ttcctgtatt
     3121 gctttaacat agggtttgaa gtcttttatt cgccatcctg tcacttcttc ttttatgcct
     3181 tctttctttt gttcttcctt tggtgattgc tgttgtgcta tggcagtcac acttaaagaa
     3241 agtgctaata cggcaacaat agtgtacaat aaaaatcttg ttgaattcat agtgaatacc
     3301 ctcttatgat aatatacact ctataattgg taattatgct attacacttt tttatcggtt
     3361 atacaaaaac aaaaattaaa tatttttcac ataataacgg tttgttgata ttttagcaat
     3421 gtaatttttt tatacatgtt cacatggagc caattggttg cattgctata accattgaaa
     3481 tagtaaaaaa atgttggaca attgttacta aataatgtat gagtgatagt aatattaatt
     3541 taaaaaattt tgcgaaaaat gaccacttac aatatttcat aatttattta gtttgctgca
     3601 ttgaaagatt tatagtgggc aactgaatag ttttttatta ttttatatca aatca
//
LOCUS       DAOSZP010000174         3642 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_20473_length_3642_cov_3.553108, whole genome shotgun sequence.
ACCESSION   DAOSZP010000174 DAOSZP010000000
VERSION     DAOSZP010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3642
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20473_length_3642_cov_3.553108"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1954)
                     /locus_tag="PK348_04875"
     CDS             complement(<1..1954)
                     /locus_tag="PK348_04875"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HXK65579.1"
                     /translation="MNNPCSVNNFWEYSLDGINCIEASAGTGKTYTISHLYLRLIIEK
                     GLSIDNILVVTFTNAATDELRSRIRSLIKIAYDMLEGRCEDAVKQKYSELYEYLQSLS
                     TNNSSNYRTQKEIRDILKIALADIDKAHIYTIHSFCSRTLSEFAFESRSLYGSKLVED
                     ETPFMQRVIEDFYYSKIAPLGEEFVALFFQKNENFVRWFMEILRKLPDDPDITIMPVI
                     PFKPKEVKQLVHTLTMQIGAIREKFNREFDAIKNNYFKALAERVLNSRSYNEEDLLTL
                     QQAIEMLSEFSVFQEDISKHIKKFSVSGLVSRLIGAYKKNEKQYTWHEFFTLLDECRK
                     TIVAIKIQIIAAYREFIQYSEKQYSWYRDRWNIISFRDILKDMHKALQQGTDSRLAVR
                     VRERFKAALIDEFQDTDPLQYTIFTTIFRKEGYTLYIIGDPKQAIYSFRGADVFAYIK
                     ARQSADTAYTLTKSFRSTPSLTRAINYIFGRVENPFVFDEIMYHPVESKIHESCFSQG
                     ALDIWFIRDSDNEKSISIGVARKIIAKNIAHEIQTLAHGSYEIDGKALQYSDIAILVR
                     KHHEAELMQDILRQYGINSVLYSRQSVFTTPEVWELLHVLEAIINPSSTKAIKRALVT
                     SLFGYCGNDIIQMEEQGTLDTVKMRCA"
     gene            complement(1951..>3642)
                     /locus_tag="PK348_04880"
     CDS             complement(1951..>3642)
                     /locus_tag="PK348_04880"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: protein motif:HMM:NF016169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HXK65580.1"
                     /translation="LGCFLNFLEALFRFFDEAQKPKKLEDWISFYDNLINTMLDVESH
                     LQDYENIQQLLSSLRAIVTDINLNEDIEFLVIKSWIDDFLSEQRVSSNFLSKGITFCQ
                     LLPMRSIPFKVVCLLGMNDGEFPRLDEHISFDILRNETHKDTLPRCIRSKRNDDRYLF
                     LESIISAQEYLFISYQGQNPKDLSLKEPSVVVSELLEYIERGFYVQDQSTSIRDVFIT
                     RHHLHHFHPAYFQEHRKYFSYSRLWFEESRALYGKKEDYKPLISQLDLPIKITDITID
                     ELIQFFKNPARHFITKVLCIYLRDEVIELAGDELLSISDNLDNYILDNEIVTWKIDGN
                     DIHQFTYIKKKEGIIPDGMLGDIIVKKKDAEVAYFANKVKQYCIGERQSYELAYTSEK
                     GITITGKPVLYGGNQVFYRYANEKVRDRLQAFLWHIFVCATMELGVTTYLIVKNKVGP
                     IVFGNISCDQAKTYLEKCIDIFIEGNKRILPFFPETSGEYYKLMNKDKGRFIQYKIEG
                     KFYEENAYNIPDYSEPYIRRAFYKVDIFRNKEVFYEFTELAEEIFTMIQECERSQ"
BASE COUNT         1105 a          715 c          541 g         1281 t
ORIGIN      
        1 cagcacagcg catcttcaca gtatccaatg ttccctgttc ttccatttgt ataatatcat
       61 ttccacaata cccaaataag cttgttacca gtgcccgctt tatggctttt gttgatgagg
      121 gatttataat tgcctcaagc acatgcaaca attcccacac ttcaggtgta gtaaatactg
      181 actgtctgct atataaaaca ctattaatac catactgtct taaaatatcc tgcatcagtt
      241 ctgcttcatg atgctttcgt accagtattg ctatatcact gtattgtaat gctttgccat
      301 caatttcata ggaaccatgc gctaacgttt gaatttcatg ggctatattt ttggctatta
      361 tcttacgtgc tacaccaata gatatactct tttcattatc gctatcccga ataaaccata
      421 tgtctaatgc tccctggcta aaacatgact catgtatctt agattcaact ggatgataca
      481 taatttcatc aaatacaaac gggttttcaa ctcgaccaaa aatataattt atagctctgg
      541 ttaacgatgg tgttgatctg aaacttttag ttaatgtata cgcagtatct gcactttgac
      601 gtgctttaat atacgcaaaa acatcagctc ctctgaagct ataaattgcc tgtttgggat
      661 caccaatgat atacagggta taaccttctt tcctgaatat tgtggtaaaa atagtatatt
      721 gtaacggatc agtatcctga aattcatcaa tgagtgctgc tttgaaacgc tccctaactc
      781 gaacagccaa tctgctgtcc gtaccttgct gtagtgcttt atgcatatct ttcaggatat
      841 ccctgaatga aatgatattc caccgatcgc gataccatga gtactgtttt tcactgtact
      901 ggataaattc acgatatgcg gctattatct gtatctttat agcaacaata gtttttctgc
      961 attcatcaag aagtgtaaaa aattcatgcc atgtatattg cttttcattt tttttgtatg
     1021 cacctatcag cctggacacc aaacctgaaa ctgaaaattt ttttatatgt ttactaatgt
     1081 cttcctgaaa cacactgaat tcagaaagca tttctatagc ttgctgtaat gtcagtaaat
     1141 cttcttcatt atacgatctg ctattcagga cgcgctctgc cagtgctttg aaataattat
     1201 tctttattgc atcaaactca cgattaaatt tttcacgaat agctccaatc tgcattgtta
     1261 gcgtatgtac cagttgtttt acttcttttg gtttaaacgg tataacaggc atgatggtaa
     1321 tatcaggatc atcaggcagt ttacgaagaa tttccatgaa ccacctgaca aagttttcat
     1381 ttttttgaaa aaataatgca acaaactcct ctccaagcgg tgctatctta gaataataaa
     1441 aatcttcaat gactctttgc atgaaaggtg tttcatcttc aacaagtttg cttccataca
     1501 atgaacggct ttcaaaagca aattcactta atgtgcggct gcaaaaactg tgaatggtat
     1561 aaatatgtgc tttatcaata tcagcaagcg ctatttttaa aatatcacgt atctcttttt
     1621 gtgttcgata gttactgcta ttgttggtag ataaagattg cagatattca tataactcac
     1681 tgtatttttg ctttacagca tcttcacatc ttccttcaag catatcgtat gcaattttta
     1741 tcaatgaacg tatccgtgac ctaagctcat cagttgctgc attggtaaat gttacaacaa
     1801 gaatattatc aatactgagt cctttttcaa tgataagccg taaatacaga tggcttatgg
     1861 tataggtttt tcctgtacct gcacttgcct caatgcagtt gatgccatct aaagaatatt
     1921 cccaaaaatt gttaacactg caaggattgt tcattgtgat ctttcacatt cctgtatcat
     1981 agtaaatatt tcttcagcta attcagtaaa ttcataaaat acttctttat ttctgaaaat
     2041 atcaaccttg taaaatgctc gtctgatata tggctctgaa taatcaggga tattatatgc
     2101 attttcctca tagaattttc cttcaatttt atattgaata aatcttccct tatccttatt
     2161 cattaactta taatattcac ctgaggtttc tgggaaaaac ggcaaaatgc gtttattccc
     2221 ttcaataaat atatcaatac atttttctaa ataggttttt gcttgatcac aagaaatgtt
     2281 tccaaaaact attggtccta ctttattttt gactataaga tatgtcgtaa ctccaagttc
     2341 catagtggca catacaaata tgtgccataa aaatgcctgc agcctatctc ttactttttc
     2401 attggcatac ctgtaaaaaa cctggtttcc accatataac actggcttgc ctgttattgt
     2461 tattcctttt tctgatgtat atgccaattc ataactttgt ctttcaccta tacaatattg
     2521 cttaacctta tttgcaaaat atgctacctc tgcatctttc tttttaacaa taatatcgcc
     2581 taacatgcca tcagggataa ttccttcttt ctttttgata tacgtaaatt gatgtatatc
     2641 attaccgtct attttccagg ttactatctc attatctaat atatagttat ccagattatc
     2701 cgaaatgctc aacagttcat cgcctgcaag ctctattacc tcatcacgca aatagataca
     2761 tagaactttt gtaataaaat gtctagctgg gtttttaaaa aactgaataa gttcatcaat
     2821 agtaatatca gtaatcttta ttggcaaatc tagctgtgaa attagtggtt tgtaatcttc
     2881 ttttttccca tataacgccc tgctttcttc aaaccacagt cttgaatagg aaaaatattt
     2941 cctgtgttcc tgaaaatatg caggatgaaa gtgatgtaaa tggtgcctgg taataaagac
     3001 atcacgtatt gatgtagatt gatcctgtac ataaaatcca cgttcaatat attcaagcaa
     3061 ttcactgaca accacagatg gctctttaag gcttaagtcc tttgggtttt gtccctgata
     3121 gcttataaat agatattcct gtgctgaaat tatgctttcc agaaataaat aacggtcatc
     3181 attgcgtttt gaacgaatgc atcgtggcaa tgtatctttg tgtgtttcat tgcgtaatat
     3241 atcaaagctt atgtgttcat caagtcgtgg gaactctcca tcattcatac caagcaggca
     3301 tacaacctta aaggggatac ttcgcatggg cagaagctgg caaaaggtaa tgcctttact
     3361 taaaaaatta ctgctgactc tttgctcaga aagaaaatca tcaatccagc tttttattac
     3421 caaaaactct atatcttcat taagattaat gtcagtaact atcgcccgta atgatgacaa
     3481 caattgttga atattttcat aatcctgcaa atggctctcc acatcaagca tggtgtttat
     3541 gagattgtca taaaagctta tccaatcttc cagttttttt ggtttttgcg cttcatcaaa
     3601 aaatctaaat agcgcttcaa gaaaatttaa aaaacatccc ag
//
LOCUS       DAOSZP010000175         3640 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_20494_length_3640_cov_3.426778, whole genome shotgun sequence.
ACCESSION   DAOSZP010000175 DAOSZP010000000
VERSION     DAOSZP010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3640
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20494_length_3640_cov_3.426778"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2197
                     /gene="ileS"
                     /locus_tag="PK348_04885"
     CDS             <1..2197
                     /gene="ileS"
                     /locus_tag="PK348_04885"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HXK65581.1"
                     /translation="PTCVTALAEAEVEYHDHSSPSIFVKFKVDPTSVTFKGVDANNLY
                     VVVWTTTPWTLPANLAVCFHPDFDYSAYTSGNEYLIMADGLAESFSAITGKTLGEKIP
                     LPIDIIRSLKVYHPFIDRESKVIFGDFVTLDQGTGIVHIAPGHGLEDYIVGLQYGLDV
                     FCPVDDEGKFTDEFALMKGVNVFDANPKIIDLLKEKNVLIFTNDIEHSYPHCWRCKQP
                     LIFRATAQWFLLIDHNDMRQLALQVTEDVQWIPEWGKLRFKSMVETRPDWCLSRQRSW
                     GVPIPSFYCKKCGKNQMNAETIFYFAKIARERSIESWYTDEIHSLIPEGFTCECGSDD
                     FEKEYDILDVWFDSGVSHFAVLDSRDDHRWPSDLYLEGSDQHRGWFQSSLWPALALRQ
                     RAPYNTVLTHGFMLDDQGRAMSKSLGNVIPPDDIINKFGADILRLWVASEDYRNDVRI
                     GYDMIQQIADSYRKIRNTFKFMIGNCADFNPSQCVHYDELSDTDKWILHKLYTLSNQV
                     RQHYEQYEFHLVYRRILNFCTVDLSSLYFDISKDILYVEAKDSKLRRANQTVLYHVTE
                     TLLRLIAPVLVFTAEEIWQFLGKESSVHADVYSELPAEWHNETLAKKMDALIDIKKDV
                     LKALEIARKEKKIGSSLEADVHIYIAGASSKELLQSMSDSNRFFQVAEVHIEDKKLDG
                     MADYDSSSILVVKSTGKKCVRCWNYSHDIGSHPDHPELCKRCTDIVLKL"
     gene            2208..2711
                     /gene="lspA"
                     /locus_tag="PK348_04890"
     CDS             2208..2711
                     /gene="lspA"
                     /locus_tag="PK348_04890"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HXK65582.1"
                     /translation="MKQETKNTLIAVLIFILILGSDIITKALVEKYISMYERVNVLGS
                     FVQLTLIYNQGGIFGILQGYQQFFLIISIIVFLLLVAYFILEKNKTLIFTVSMGLICA
                     GAIGNILDRITGKPGVVDFIYIGSDDVFRWPAFNIADSSIVVGATLLVIHQLIEEKKR
                     KAQNKYN"
     gene            complement(2712..3110)
                     /locus_tag="PK348_04895"
     CDS             complement(2712..3110)
                     /locus_tag="PK348_04895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65583.1"
                     /translation="MMRIYRVHFYKLLLCVAVISMVTCGKEAPLQFCEGVDKDGKPVH
                     CGKEFTTGDMTLYVMAQEPFGSNTATVIVYGKKKYSQPEYTRFDYTINPDGNTATIPL
                     SLYAEGTYIVKVIVKDKEIASGEITVIDTY"
     gene            complement(3079..3366)
                     /locus_tag="PK348_04900"
     CDS             complement(3079..3366)
                     /locus_tag="PK348_04900"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002973674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HXK65584.1"
                     /translation="MEKKAKKITFEDALEELEAIASKLESGELTLEQSIQYYERGVEL
                     AKFCRQKLEEAERKIEVLQKQPDGTVQKKSVKVKEDTGEIEDDEDIQGSLL"
     gene            complement(3350..>3640)
                     /locus_tag="PK348_04905"
     CDS             complement(3350..>3640)
                     /locus_tag="PK348_04905"
                     /inference="COORDINATES: protein motif:HMM:NF014640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HXK65585.1"
                     /translation="TDTKERLSRVRDLELIIKNRLQNYWHIFMMNASKLEHLSPVSVL
                     KRGYSITLKDSQIVSSIKKIHQGDTIRVIVYDGSMQCDIQLVNEEVSFGKKG"
BASE COUNT         1026 a          779 c          732 g         1103 t
ORIGIN      
        1 cccaacctgt gtaacagcac tggccgaagc cgaagttgaa tatcatgatc actcatcacc
       61 gtcaattttt gttaagttta aagtggatcc cacatccgta acattcaaag gggttgatgc
      121 taacaacctg tatgtggtgg tatggaccac aacaccatgg acgttacctg ccaacctggc
      181 tgtttgtttt catccagatt ttgattactc agcttatacg tcaggtaacg agtatttaat
      241 tatggcagat ggacttgcgg aatcattttc cgccataact ggaaaaacct taggtgaaaa
      301 gataccctta ccaattgaca tcatacgctc actgaaagta taccatcctt ttattgacag
      361 ggaatcaaaa gtcatctttg gcgattttgt tacactggat cagggtaccg gcattgtcca
      421 tattgccccg gggcatggac ttgaggacta cattgtaggt cttcaatacg ggcttgatgt
      481 attctgcccg gttgatgatg aaggtaaatt taccgatgaa tttgccctca tgaaaggcgt
      541 caatgtcttt gatgctaacc caaaaataat agatctttta aaagaaaaaa atgtactgat
      601 ttttaccaat gatattgaac attcatatcc ccactgctgg cgctgcaaac agccgctcat
      661 cttcagggct accgctcagt ggtttttact cattgaccat aatgatatgc gacagttagc
      721 tttacaggta actgaagatg tacagtggat ccctgaatgg ggtaaattgc ggtttaaaag
      781 catggtagaa acacgccctg actggtgcct ttcacggcag cgttcatggg gggttcccat
      841 tccttccttc tactgtaaaa aatgtggaaa aaaccagatg aatgctgaaa ccatctttta
      901 ttttgcaaag attgctcgag agcgcagcat tgaatcatgg tataccgatg agatacacag
      961 tcttattcct gaaggcttta cctgcgaatg cggaagcgac gattttgaaa aagaatatga
     1021 tattctggat gtatggtttg actcaggcgt ttcacatttt gcagtgcttg acagccggga
     1081 tgaccaccgc tggccatccg acctgtattt agagggaagc gaccagcatc gaggctggtt
     1141 ccagtcttca ttgtggccag cacttgcact gcgccagcgg gctccatata ataccgtact
     1201 gactcacggt ttcatgttag acgatcaggg gcgggcaatg agtaaatccc ttggtaatgt
     1261 tatcccgcct gatgatataa tcaacaagtt tggtgccgat atcctgcgtc tgtgggttgc
     1321 ttccgaggac tatcgcaatg atgtcaggat tggttacgat atgatacagc aaattgcaga
     1381 ttcataccgt aaaatccgca acacctttaa attcatgata gggaactgtg ctgattttaa
     1441 tccttcacag tgtgtccatt acgatgagct atcggacact gataaatgga tactgcataa
     1501 actctatacc ctttccaatc aggtgcgcca gcactatgaa cagtatgaat ttcaccttgt
     1561 gtaccgccga atactgaact tctgtacggt ggacctttca tcactgtatt ttgatatttc
     1621 aaaagacatc ctttatgtag aagcaaagga ttccaaattg cgaagagcaa atcagactgt
     1681 cctgtaccat gttaccgaaa cactgcttcg actcattgca ccggtactgg tgttcactgc
     1741 cgaagaaata tggcaatttt taggcaaaga atcttccgtg catgctgatg tatacagtga
     1801 actgcctgcg gaatggcaca acgaaacact ggcaaagaag atggatgctc tcattgacat
     1861 caagaaagat gtattgaagg ctttagagat agctcgaaag gaaaagaaaa ttggtagctc
     1921 gcttgaggcg gatgttcaca tatatattgc aggtgcgtca tcaaaagagc ttttgcagtc
     1981 aatgagcgat agtaaccgct ttttccaggt ggccgaagtg cacattgaag ataaaaaact
     2041 ggatggcatg gccgattatg acagtagctc catactggta gttaaatcaa cagggaaaaa
     2101 atgtgtcagg tgctggaatt attcgcacga cattggttca cacccggatc acccggaact
     2161 ttgcaaacga tgcaccgata ttgtattgaa gctgtaagga taccacaatg aaacaagaaa
     2221 caaaaaacac attgattgca gtgttgattt ttattcttat tttaggaagc gacattataa
     2281 caaaagcgct tgtggaaaaa tatatttcta tgtatgaacg ggtcaatgtg ctgggtagct
     2341 ttgtacaact gacacttatc tacaatcagg gtgggatatt tggtatacta cagggatatc
     2401 agcagttttt ccttattatt tcaattattg tgtttttgct tcttgttgca tactttatac
     2461 ttgaaaagaa caaaacgctt atctttactg taagcatggg acttatctgc gcaggcgcca
     2521 taggcaatat tttagacagg attaccggta aacccggggt ggttgatttt atctacattg
     2581 gtagtgatga tgtttttcgc tggccagcat ttaatattgc tgattcttca attgtggtag
     2641 gtgcaacact tcttgttatc caccagctta ttgaagagaa aaaacggaaa gctcaaaata
     2701 aatacaatta attaatacgt atcaattaca gtaatttcac cacttgctat ttctttatct
     2761 ttgactatta ctttcactat atacgtaccc tcagcataca gggacaatgg tattgtagca
     2821 gtattaccat caggatttat ggtataatca aagcgggtat actcaggctg gctgtatttc
     2881 ttttttccat acacgattac cgttgctgta ttacttccaa aaggctcctg tgccataaca
     2941 tacagcgtca tatcacctgt ggtaaattct tttccgcaat gaacgggttt gccatcctta
     3001 tcaacacctt cacaaaactg cagtggtgct tctttgccac aggtaaccat gctgataaca
     3061 gccacacaca gcaacaattt atagaagtga accctgtata tcctcatcat cttctatttc
     3121 ccctgtatcc tctttaactt taaccgattt tttttgtacc gttccatcag gctgcttttg
     3181 caacacctca atcttgcgtt ctgcttcttc taatttttgc cggcaaaact ttgccagttc
     3241 aacacccctt tcatagtact gtatggactg ctccagcgtc agttcaccag actctaactt
     3301 tgatgctatt gcctcaagct cttccagcgc atcctcaaag gttattttct tagccttttt
     3361 ttccaaagct cacctcttca tttaccaatt gtatatcaca ttgcattgac ccatcataga
     3421 caataaccct gatagtatca ccctggtgga tcttctttat tgaagaaact atctgtgaat
     3481 ctttcagtgt aatggaataa ccccgcttca gcacacttac cggtgaaagg tgctccagtt
     3541 tgcttgcatt catcataaag atgtgccagt agttttgcaa tctatttttt ataataagct
     3601 ctaaatccct tacacgactt aaccgttctt ttgtatccgt
//
LOCUS       DAOSZP010000176         3637 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_20509_length_3637_cov_3.396427, whole genome shotgun sequence.
ACCESSION   DAOSZP010000176 DAOSZP010000000
VERSION     DAOSZP010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3637
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20509_length_3637_cov_3.396427"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..539)
                     /locus_tag="PK348_04910"
     CDS             complement(<1..539)
                     /locus_tag="PK348_04910"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HXK65586.1"
                     /translation="MKPTYILTHDVGTTGNKTCLYKVDNKITLIDSYLVEYPLYMLPD
                     GGVEQKADQWWDAICKATKMVMKKSGIPPAKIAAMAFCAQMQGSVFIDKDGNALRNPM
                     SYLDQRATRQIEKYLYKGIVKIEKWNAYKTIKSLIVTGGLAATPKDPLWKYHWVKDNE
                     PDIFAKAYKWLDVKDYLIF"
     gene            complement(681..1811)
                     /locus_tag="PK348_04915"
     CDS             complement(681..1811)
                     /locus_tag="PK348_04915"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXK65587.1"
                     /translation="MIPFISIPPLLMAAIAVYVAVSYTLMFARRKNEPEHIWFALMCY
                     TIALYDIFSALLYMSPSPLHSILYQRMQFAVLACFIIAITWFIASLVHINSTLVYTIT
                     LIMGLFIIAGFVFDSSYTLNSDNPYIKQFHFFGTTITYNEADPGLVYVLQYIAMVITG
                     LYLLYKLFIWCRYGEIHIKILFISFSIFLLASINDVCVGKGMYQFIYILEYAYFFVIL
                     SMAYILQSRFIKLHREIEHLTFLFNRKLLEKERYLQPISSESKHKQLSATNSEKIQHA
                     VEYIHANFAFDLSREGLAAMIDLHPDTFSRLFKTYTGKSLPDYINELRINYARELLTS
                     TNDSIVTIAFKTGFESLSTFNRAFHKILNTTPTAYRKHNSKF"
     gene            complement(1998..2129)
                     /locus_tag="PK348_04920"
     CDS             complement(1998..2129)
                     /locus_tag="PK348_04920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65588.1"
                     /translation="MPSGKMFLTFNGDLSLKGNGYGSKASIVDANSEDTLDGELDLF"
     gene            complement(2258..2461)
                     /locus_tag="PK348_04925"
     CDS             complement(2258..2461)
                     /locus_tag="PK348_04925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65589.1"
                     /translation="MTEILYSLPLCYAKVTAMLIFLGMLVVAWLLPYNFVVQGAPDKK
                     RWRDLRLWATLLTIVQLFIYYIF"
     gene            complement(2477..>3637)
                     /locus_tag="PK348_04930"
     CDS             complement(2477..>3637)
                     /locus_tag="PK348_04930"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Members of the Solute:Sodium Symporter (SSS), TC
                     2.A.21 as described in tcdb.org, catalyze solute:Na+
                     symport. Known solutes for members of the family include
                     sugars, amino acids, nucleosides, inositols, vitamins,
                     urea or anions, depending on the system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/solute symporter"
                     /protein_id="HXK65590.1"
                     /translation="YSIPEYLGLRYNNAVRFIAACVMSVFAVVIVGVFLWATAIMLQT
                     YLGWPIWFSILVTATVVGFYTISGGLGAVAITDTVQTFIMFFTAIIVAFIGIEKIGGF
                     DMFIKTLQNNYPDHLHSFLPANHPVFPWPGVILGLGIVLSPAYWCANQAILQRTFAAK
                     SEWDGKASMMFAACLKTFVPFLIVMPGLLALTLAGKSITHQDQALPWVIKHILPAGIS
                     GLMFVAFIAALQSSIDSTMNSTSVMIVRDIINVAKKTKLTDRAQLRLGKLFTFIILIV
                     GIAFAPITAYFQGIYVYVQYALSLFQGPIFALMLFGILDKRITPSAGLFSMISGLAVA
                     AVLGWLQFNMLYIAFLSFVYSAIALYIVSLFTKRKPEAELKNVVYSNVVEID"
BASE COUNT         1249 a          661 c          645 g         1082 t
ORIGIN      
        1 ctgaaaatca gatagtcctt aacatcaagc cacttgtatg cttttgcaaa aatatcaggt
       61 tcattatcct tgacccagtg gtatttccat aatgggtctt ttggcgttgc agcaagccct
      121 ccagttacaa ttaatgattt gatagtttta taagcattcc atttttcaat tttaacaatt
      181 cctttgtaca gatatttttc aatttgcctg gtagcacgtt gatctaaata actcatgggg
      241 ttacgcaatg cgttgccatc tttgtcaata aacacagagc cctgcatctg tgcacagaat
      301 gccatggcag caatctttgc aggtggaata ccgcttttct tcattaccat ctttgtggcc
      361 ttacagatgg catcccacca ctggtcagct ttttgttcaa cacctccatc aggaagcata
      421 tacagaggat attccaccag atacgaatca ataagtgtta ttttattatc aactttataa
      481 aggcatgttt tgtttcctgt ggtacccacg tcatgagtta atatatatgt tggtttcatt
      541 ttgatgcctc cagaaataat acttttaaac ttattttttt aaccgcctta catagcgatt
      601 ctacattaca ttgcaaaagg atcttagaat gcttttttat aggatataaa tgtgtatttg
      661 tctacgcaat atctgattaa tcagaatttg ctattatgtt tacggtaagc tgttggagtg
      721 gtattaagaa ttttgtgaaa tgcacggtta aacgtggaaa ggctttcaaa gccagtttta
      781 aaggcgatag ttactattga atcattggtt gatgtaagca attcccgtgc gtaattaatt
      841 ctcaattcat ttatgtagtc agggagggat ttacctgtgt atgttttaaa aagcctgctg
      901 aatgtatcgg gatgcaaatc aatcattgca gcaagccctt ctcgagatag atcaaatgca
      961 aagtttgcat gaatgtactc aacggcatgc tgaatctttt ctgagtttgt tgcagaaagc
     1021 tgtttatgct tgctttcact ggatattggt tgtaaatatc tttctttttc cagaagcttt
     1081 ctgttaaata aaaaagtcag atgttctatc tccctgtgga gcttaatgaa gcgtgattgt
     1141 aaaatgtatg ccatggaaag aatgacaaaa aagtaggcat attcaagtat gtaaataaat
     1201 tggtacatac ctttaccaac acatacgtca ttaatgctgg caagaaggaa aattgaaaaa
     1261 ctgataaata gaatttttat gtgaatttca ccatagcggc accatataaa aagtttgtat
     1321 aataaatata aaccagttat taccatggca atatactgta acacatacac aagaccagga
     1381 tcagcttcat tgtacgttat tgttgttcca aaaaaatgaa attgctttat atatgggttg
     1441 tcagagttta atgtatatga actgtcgaaa acaaatccag ctatgataaa taaccccatt
     1501 atcaatgtga tggtgtaaac taaagtagaa ttaatatgca ccagtgaagc aataaaccaa
     1561 gttattgcta taatgaaaca tgccaataca gcaaattgca tacgttgata cagtattgaa
     1621 tgaagtggtg atggcgacat atacagcaaa gcggaaaata tatcatacaa tgcaattgta
     1681 taacacatta atgcaaacca tatatgttcg ggttcatttt ttcttcgtgc aaacatgaga
     1741 gtgtatgata ctgcaacata cactgctata gcagccataa gtaggggagg aatggaaata
     1801 aatggtatca tgataataaa atttcaatgc attataaaat aattgtgaaa taaatgatat
     1861 atttataaaa aataactaaa ctaaaatggc aagaaaataa taaaaaaata aataaaggtg
     1921 agtagacgta tactcacctt tcattgtaaa actaaaacta aaaaatatct ttgtttttca
     1981 atacacagca gttattccta aaacaagtcc aattcaccat ccaacgtatc ttcactgttt
     2041 gcatcaacta ttgatgcttt actcccgtag ccattccctt tcagggataa atccccgttg
     2101 aaagtcaaaa acattttccc tgatggcata aaatatgcat attcttcccg cgctgaaaca
     2161 tatgaactta ataataaaat aatagaagca accagggtaa aaaaatattt tgtttttaat
     2221 atgtatcgta gtgagtttat gatttgctat atactattca aaaaatataa taaataaata
     2281 actgtactat agttaataag gttgcccaca gtcgtaaatc gcgccatcgt tttttatcag
     2341 gagcaccctg gacaacaaaa ttatacggca aaagccaagc aacaacaagc atgcctaaga
     2401 aaataagcat tgctgtaact tttgcatagc aaagcggaag tgaatacagt atttctgtca
     2461 tacgggtctc caccaattaa tcaatttcaa caacattgct atacacaaca tttttcagtt
     2521 cagcttctgg ttttcttttc gtaaacaggg aaacaatata cagagctatc gccgaataca
     2581 caaatgaaag aaatgctata tacagcatgt tgaactgtaa ccagcctaag actgctgcaa
     2641 cagccagccc cgatatcatg ctaaaaagac cagctgaagg tgtaatacgt ttatctaaga
     2701 taccaaagag catcagtgca aaaattggcc cctgaaaaag ggataacgca tattgcacat
     2761 acacataaat accctgaaag taagctgtga ttggtgcaaa tgcaatgcct acaataagaa
     2821 tgataaatgt aaacaatttc cccagacgca attgtgctct gtcagtcagt ttagtttttt
     2881 tggcaacatt aataatatcc cgaacaatca taactgaggt ggaattcatg gttgaatcta
     2941 tcgatgactg taaagcagca atgaatgcaa caaacataag gcctgaaatg cctgcaggta
     3001 gaatatgttt aataacccat ggcaatgcct gatcctggtg ggtaatactt ttgcctgcaa
     3061 gggtcagagc aagtagccct ggcattacaa ttaaaaatgg tacaaacgtt ttgaggcatg
     3121 cagcaaacat cattgaagct ttgccatccc attcactttt ggctgcaaag gttcgctgta
     3181 aaattgcctg attggcacac cagtatgcgg gagaaagcac aatgccaagt cccagtatta
     3241 cgcctggcca tgggaatact gggtgattgg caggcaaaaa tgaatggagg tggtcagggt
     3301 aattgttttg taatgttttt ataaacatat cgaaaccacc aattttttca atacctataa
     3361 aagcaactat aatggctgta aaaaacataa tgaatgtctg aacggtatcg gtgatagcaa
     3421 ctgcgccaag cccgcctgat atagtataaa agccaacaac ggttgctgtt accagtattg
     3481 aaaaccatat tggccagccc aaatatgttt gcagcataat ggcggttgcc cacagaaaca
     3541 ctccaacaat aaccactgca aatacactca taacacaggc cgcaataaaa cgcacagcat
     3601 tattatatcg caagcccaaa tattcaggta ttgaata
//
LOCUS       DAOSZP010000177         3632 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_20543_length_3632_cov_3.466033, whole genome shotgun sequence.
ACCESSION   DAOSZP010000177 DAOSZP010000000
VERSION     DAOSZP010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3632
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20543_length_3632_cov_3.466033"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..793)
                     /locus_tag="PK348_04935"
     CDS             complement(<1..793)
                     /locus_tag="PK348_04935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65591.1"
                     /translation="MRTLLQHIDTLEKKERYTLARMLGFQDPKKTTTKDIASLMSSHV
                     GLELIFSQCNPDELSILKLLSKNDVHFRDIEHNLHIPIYQIEKSAQLLASKSIVYILK
                     NRQLLSNKLDKICLYNELRQTLNPCDTHTLYEYCNEIIKHITSTKSHIPNKPHLSENA
                     SSFLKLCIQRGCVVSVNYAISTLGIIKANSVLNELTINKCIRIFHSISTHFSGIIMVH
                     PDYIQIKNESKNIHIHNHFMALIAMLKAYDCISSKGLFLTQEGNLR"
     gene            1016..2086
                     /gene="lepB"
                     /locus_tag="PK348_04940"
     CDS             1016..2086
                     /gene="lepB"
                     /locus_tag="PK348_04940"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HXK65592.1"
                     /translation="MLQFLALNKQNTDTIQIQVLSNVQITKLMLKRIGYLILLIYSTI
                     FLAVAIPYSGTYAIGGFLCSVFMLRILNDFIKWRKFENATLTASPSGVSIQTKNENYH
                     FNAKDITYLEVNFFSNLVIRERYRSLGFPLMLVSNDDREKLIHYFYDMSPKRTVMFKK
                     IWEMFDAILVAFILAMHIRQFIIQAYYIPTGSMEDTLLVGDHLLAEKITYGPTIPQMI
                     GMKKPIHLSFLSIRPVQRGDIIIFRPPNEEEKDFIKRCIAVEGDEVHIDDGAVWVNGV
                     KLDEPYVKGVTSYRGFSEKRIEGVVPKGMIVAMGDNRENSYDSRGFGYVPLDRIKGKA
                     FILYWNTDNIKNLDFSRLGLIK"
     gene            2101..>3632
                     /locus_tag="PK348_04945"
     CDS             2101..>3632
                     /locus_tag="PK348_04945"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HXK65593.1"
                     /translation="MENVSHKRRIIITLIFISCIAVLLILKITQLHFSSRVIVNNKHD
                     IIQRGDIIDANGYPLAVSIYKYSIFADPRKVIDPDAESLKLSSILAIPESEIKDLLTR
                     DKHFVWIKRKVDDNVINKVKELSLPGIYVKKEYARAYPEGTLASQVIGFVNIDNIGLE
                     GIEYKYNDVLLYTDSSGEKEQKGLTVQLTLDRAVQYIAQKAINTYAEKVKPKKAVAVV
                     YEIKTGKIMALAVYPQFHPERFYNYDKSVLTCFSVVDAFEPGSTMKIFAAITLLEHLP
                     DALKRTYICKGSVDVYDATIKCTKEHGKVSLNDIITYSCNAGIIQAMQPVKKEYLYQT
                     LTRFGFGEKTGIQLPGESQGILRNTKQWSGLSKYSMAIGQEISVTSVQLVAAFGAIAN
                     NGIYMYPQIVNQIKSHYGETVTKFFPKTKGKIVNETIAKTILSMMHNVVIEGTGKKAF
                     VAGYGTGGKTGTAQKSLPQGGYIPDAYVVSFVGIAPVDNPDICILVMFDEPAYGGSGG
                     ELA"
BASE COUNT         1192 a          572 c          710 g         1158 t
ORIGIN      
        1 tgcgtaaatt tccctcctgt gttaaaaaca gcccctttga agatatgcag tcataagcct
       61 ttaacatagc aataagtgcc atgaaatgat tgtgaatatg tatattttta ctctcatttt
      121 tgatttgtat ataatcgggg tgaaccataa taatgcctga aaaatgtgta gatatactat
      181 gaaaaattct gatgcactta ttaatagtta attcattcaa tacactattg gcttttataa
      241 taccaagagt ggatattgca tagttcactg aaacaacaca tcctcgctgt atacaaagtt
      301 ttaaaaaact tgatgcattt tcactcagat ggggcttgtt gggtatatgt gatttagttg
      361 atgtgatgtg tttgattatt tcgttacagt attcatataa tgtatgggta tcacaaggat
      421 tcagtgtttg ccgcagttca ttgtataaac atattttatc taatttgtta gatagtaact
      481 gtcgattttt caggatgtag acaatggatt tactggccaa taactgagca cttttttcaa
      541 tctgatatat gggaatatgc aggttgtgct caatatccct gaaatgtaca tcattttttg
      601 acaaaagctt gagtattgaa agttcatcag gattacattg tgaaaaaatc agttcaaggc
      661 caacatgact tgacatgagt gaagctatat ctttagttgt tgttttttta ggatcctgga
      721 agccaagcat tctggcaaga gtgtagcgct cttttttttc taatgtatcg atatgttgta
      781 ataatgttct cattatttaa aaaatagtag acacattaat cgtgataata tacatgatat
      841 cttcaataat tattccttgc aataatttta tgatagatga aatgtgcatc actgcttcaa
      901 gtggtctgga tgtattgtat tccataattt attcataaat atttcaatta aaatgtaact
      961 tttataaaag aattgcatat acaacatata tccttataaa agccgaggta catagatgtt
     1021 acaattctta gctcttaaca aacaaaatac tgatacaatt caaatacagg ttctaagcaa
     1081 cgttcagatt accaagctca tgttgaagcg aataggatat ctgatacttt taatttattc
     1141 aactattttt ttggcggtag caataccgta tagtggcaca tacgctatag gtgggttttt
     1201 atgtagcgtg tttatgctcc gaatacttaa tgattttatt aaatggcgaa aatttgagaa
     1261 cgctacatta accgcatctc catcgggagt aagcattcaa accaaaaatg agaactatca
     1321 ctttaacgca aaagatatta catacctgga agtcaatttt tttagcaacc tggtaatacg
     1381 tgaacgttac cggagtttag gattcccact catgctggta agcaatgacg acagggaaaa
     1441 acttatacat tacttttatg atatgtcgcc taaacgcact gtaatgttta aaaaaatatg
     1501 ggaaatgttt gatgccatcc ttgttgcatt tatccttgcc atgcatatac gacagtttat
     1561 tattcaggca tattatatcc caaccggttc catggaagat accttacttg ttggggatca
     1621 ccttttagca gaaaagataa catatggacc aactatccct caaatgattg gaatgaaaaa
     1681 gccaatacac ctttcttttc tgagtatacg gcctgttcag cgtggtgata tcattatatt
     1741 ccgtcctcct aatgaagaag aaaaagattt tataaagcgg tgtatagcgg tagagggtga
     1801 tgaagtgcat attgatgacg gtgccgtatg ggttaatggt gtaaaattag atgagccata
     1861 tgtcaaaggt gtcacgtcat accggggatt cagcgagaag cgtattgaag gagttgttcc
     1921 taaagggatg atagtagcaa tgggcgataa cagagaaaat tcatatgaca gccgtggttt
     1981 tgggtatgta ccattagaca ggataaaagg taaagccttt attctatact ggaatacgga
     2041 taacattaaa aatttagatt tttcaaggct tggtttaatc aaataacatt caaggctgat
     2101 atggagaatg tatcccataa acgaagaata attattacat taatctttat cagttgtata
     2161 gccgtattgt taatactaaa aattacacaa ctgcatttca gttccagagt aattgttaat
     2221 aataaacatg atataataca gcgtggggat atcattgatg caaacggata tccccttgct
     2281 gtttcaatat ataaatattc tatatttgct gatccccgta aagttatcga tcctgatgct
     2341 gaatcattga aactttcatc tatccttgcc attcctgagt ccgaaataaa agatctatta
     2401 acccgtgaca aacattttgt gtggattaaa cgaaaggtag atgataacgt gattaacaaa
     2461 gtaaaagaat tatctttgcc gggtatttat gttaaaaaag aatatgcacg tgcatatcct
     2521 gaaggcacat tagcatcgca ggttatcggt tttgttaata ttgacaacat agggcttgaa
     2581 ggtattgaat ataaatataa tgatgtgtta ttgtataccg atagttctgg agaaaaagaa
     2641 caaaaggggc tgacagtaca gctgacactg gacagagcag tacagtatat tgctcagaaa
     2701 gcaattaata cctatgcaga gaaggtaaaa cccaaaaaag ccgttgctgt ggtttacgaa
     2761 attaagacag gaaagatcat ggcgcttgca gtatatccac aatttcatcc tgaacgtttt
     2821 tataattacg ataaatctgt gttaacatgc ttttctgttg ttgatgcgtt tgaacctgga
     2881 tcaacaatga agatatttgc tgctataacc ttactggaac atctaccgga tgcgctgaaa
     2941 agaacgtata tctgcaaagg cagtgttgat gtatatgatg caactataaa gtgcacaaag
     3001 gaacatggca aagtttctct gaatgatatc attacgtatt catgtaatgc aggaatcatt
     3061 caggcaatgc aaccggtaaa aaaagaatat ttatatcaaa ctcttacccg ctttggattt
     3121 ggtgaaaaaa caggtataca gcttccaggt gaatcacagg gtattcttcg taatacaaag
     3181 caatggtctg gtctttcaaa gtattcaatg gcaatagggc aggaaatatc agtgacctct
     3241 gtgcaacttg ttgctgcttt cggagctatc gctaacaatg gcatatatat gtatcctcag
     3301 atagtaaatc agattaaaag ccattatggt gaaacagtaa ctaaattttt cccaaagaca
     3361 aagggaaaaa tagtgaatga aacgatagca aaaaccattc tttctatgat gcataatgtt
     3421 gttattgagg gaacaggtaa aaaagcattt gttgcaggct atggcacagg ggggaaaacc
     3481 gggacagctc agaaatcatt gccacagggt ggctatattc ctgatgcgta tgttgtatca
     3541 tttgtaggga tagctccggt tgataatcct gatatttgta ttcttgttat gtttgatgag
     3601 cctgcgtatg gcggttcagg tggtgagctg gc
//
LOCUS       DAOSZP010000178         3631 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_20551_length_3631_cov_3.394575, whole genome shotgun sequence.
ACCESSION   DAOSZP010000178 DAOSZP010000000
VERSION     DAOSZP010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3631)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3631)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3631
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20551_length_3631_cov_3.394575"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            154..939
                     /locus_tag="PK348_04950"
     CDS             154..939
                     /locus_tag="PK348_04950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65594.1"
                     /translation="MKRVLCFASLVLLIFISIPVFAQQNNFMISPPPIPWFEFKEGQK
                     DLRVTATGMYITGDSTPPLTGDVKVWGAGAGGIYRYAFKDILAFDVGLMLVGATGDVG
                     NEVDMSMWLASLPLDLEILLVNNDQISLIGFIGFGFSRNYIGIDSNLPGYEGTVDITT
                     TMKGPQGGLQISFKLQDFTISPFVMITRQSGNVDMDYDIEGQGSGSISVTAPTITSRY
                     FGFDIVYVPLGLSLSSLMQQVSESGDNAGYKTYVINIGYCTQL"
     gene            1024..1617
                     /locus_tag="PK348_04955"
     CDS             1024..1617
                     /locus_tag="PK348_04955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65595.1"
                     /translation="MKYTVKLIFAVLVCLTMVSFGMAAENLDNRTRDIGLYGGVLLPG
                     TISIEDADEDTEMGFTLKAYFDQFVSPMFSVGVYGQYAATSTDSPEIVNPSVDVDVYE
                     FGLTFKPKFMISPTAAIKPGLEIGYRSTTVDLSQPGIDTSADGLALNFSAELQFALDG
                     GYLLGLTFGFLAQPTGGTDDADVTWAPIWYAMAGIVF"
     gene            complement(1684..3631)
                     /locus_tag="PK348_04960"
                     /pseudo
     CDS             complement(1684..3631)
                     /locus_tag="PK348_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417406.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
BASE COUNT          971 a          801 c          709 g         1150 t
ORIGIN      
        1 tattagttgt gcaggttgct gtcgtttaaa aatattttca attattaaat gttaaaatca
       61 tctaccttac caaaacttgc ataatataat aaaaccacca tggcttaatt tacccctatc
      121 tatttaacaa atatattaca gtggaggttt gccatgaaaa gggtactatg ttttgcaagt
      181 cttgttttgt taatctttat ttccattcct gtctttgctc agcaaaataa cttcatgatc
      241 tccccaccac ctattccatg gtttgagttc aaagaaggcc agaaagactt acgtgttaca
      301 gcaaccggga tgtacatcac tggtgatagc acaccaccac tcaccggcga tgttaaggta
      361 tggggtgctg gtgctggcgg tatttaccgg tatgcattta aagatatcct ggcatttgat
      421 gttgggctga tgctggttgg agcaaccggc gacgttggca atgaagtgga tatgagcatg
      481 tggcttgctt ccttaccact ggatttagaa atactgttag ttaataatga ccagatatca
      541 cttattggct ttattggttt tggattttcg cgaaattaca tcggaataga ttcaaattta
      601 ccaggttatg aaggaactgt tgacataacc acaaccatga aaggacccca gggtggacta
      661 cagataagtt tcaaattaca ggacttcacc ataagtccat ttgtcatgat tacccgacaa
      721 tccggtaatg ttgatatgga ttatgatatt gaaggtcagg gctctggctc tatctcagtt
      781 actgcaccca caataacaag ccgctatttt ggttttgata ttgtatatgt accactggga
      841 ctttcactga gttccctgat gcagcaggta agtgaaagcg gcgataatgc aggatataaa
      901 acctatgtta taaacatagg ctattgcaca cagttatagt attttggata ttccttttat
      961 aatcatttca atgactataa aagattatta aaattttaaa aaattaattt tgtggaggtt
     1021 tttatgaagt acactgtaaa acttatcttt gccgtgcttg tctgcttaac aatggtatcg
     1081 tttggaatgg ctgcagaaaa tcttgacaac aggacaagag acattggcct ttatggtggt
     1141 gtattattgc cgggcactat tagcattgaa gatgctgacg aggatactga gatgggcttt
     1201 actctgaaag cctattttga tcagtttgtt tcccccatgt tttcagttgg tgtatatgga
     1261 cagtatgcag caacatcaac agattctcca gagatagtaa atccaagtgt tgatgttgac
     1321 gtgtatgaat ttggtcttac atttaaaccc aagttcatga tatctccaac tgcagctata
     1381 aaaccaggcc ttgaaatagg ataccgaagt acaactgtag atttatctca gccagggatt
     1441 gatacaagtg cagatggtct tgctcttaat ttctcagctg agcttcagtt tgcgcttgat
     1501 ggcggatatt tactggggct tacctttgga tttttagccc agccaaccgg aggtaccgat
     1561 gatgcagatg taacctgggc acctatatgg tatgcaatgg caggtattgt attttaaata
     1621 attaaattaa ataatgttag taacatttgc gcaccgcctt caaacggtgc gcaaacgttt
     1681 ttgttacaca accttttttt cctgcttgct tcgtttaaca ttaaattgaa tctggccgtt
     1741 tttaaagtca acaagcacag tatcatcatc catcacttcg ccagctaaaa tcttaactga
     1801 aagcgggttg agtaaatgcc gttgcattgc acgcttaagc ggtcgtgcac caaactgcgg
     1861 atcaaagcca acttcaacaa ggtagtccag tgcttttggt gttaattcaa ggcttatgcc
     1921 gcgctctttt aaccgtttac caacttcatt aagctgtatc ataataatct tgttgatgtg
     1981 ctcacgggtt aacgggttga atattatgat gtcatcaatc ctgtttaaaa actcaggctt
     2041 aaagtgctgc ttcaactcct gttgcacgcc ttttacccgc tgttcataag gtattgatgc
     2101 atctgatata tacgctgtcc caatgttaga tgtcatgata atcagcgtat ttttaaaatc
     2161 aaccgtgcgc ccttttgagt cggtcagtcg cccttcatca agtatctgta aaaagatatt
     2221 gaatacatca gggtgagctt tctcaatttc atcaaacagc accactgagt atggtcttcg
     2281 tcgtattgcc tcggttaact gaccaccttc ctcatagcca acgtaaccag gtggtgcacc
     2341 aataaggcgc gcaaccgcat gtttttccat atattcagac atgtcaatgc gtactatggc
     2401 cttttcatcg ttaaataaaa attcagctat ggcttttgcc gtctcggttt tacctacacc
     2461 cgttggtcct aagaatacga atgaaccaat tggcctgtta ggatcctgca atcctgaacg
     2521 gctgcggcgt accgcatcag ctattgtctg tactgcctgc tcctggccaa ccacacgctt
     2581 ttcaataatt tcttacattt tcaggagctt ttctttttca ccttcaagca tcttgcttac
     2641 cggtattccg gtccagcgtg aaacaacggc agctatctcc tgctccgtaa cttcctcttt
     2701 cagcagtttg cgctctttct gaatctgtgt cagcttatca ttaagagctt ttagcttctt
     2761 ctcgtgctca ataattttac cataccgtat ttcagcaacc aggttaagat tgccctcacg
     2821 ttcagccttc tgttcctgag cacgcaggtt ttcaatctct tccttaattt tgcgtatttc
     2881 caaaatgatg ttcttttcat tttgccactg taccgaaagc tccttcttct tttcattgag
     2941 attagccaga tcttcctcaa tctttgcaag ccgttcaagt gaggctttat ccttttcttt
     3001 tttcacggct tcgcgttcaa tagtaagttg ccgaattttt cgttcaattt catcaagctc
     3061 tatgggcatt gaatcaattt ccatcttaat gagtgaagcg gcctcatcaa tgaggtcaac
     3121 agctttgtcg ggcaaaaaac ggtcggtgat gtagcggttt gaaagtacag cagcagcaac
     3181 aactgcggaa tctgaaatcc ttacaccatg atgaacctca tagcgctcct ttaatccacg
     3241 taatatggcg atagtatcct ctactgttgg ctggccaaca aacactggtt gaaaacggcg
     3301 ctccagtgcc ttgtcctttt caatatattt ctggtattcg ttaagtgtcg ttgcacctat
     3361 acagcgcagt tcaccacgag ccagtgcagg tttaagcata ttggatgcat ccaccgaacc
     3421 ttcggccgca ccggcaccaa ctaacacgtg aagctcatca ataaaaagaa tcacatcgcc
     3481 ttccgactct tccacttcct gaatcacagc cttcaagcgc tcttcaaact cgccacggta
     3541 tttagcacca gcgataagtg ctcccatatc aagaccaact actagcttgt tcttaaggct
     3601 ttcaggaaca tctccggcaa caatacgctg g
//
LOCUS       DAOSZP010000179         3626 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_20579_length_3626_cov_3.886866, whole genome shotgun sequence.
ACCESSION   DAOSZP010000179 DAOSZP010000000
VERSION     DAOSZP010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3626
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20579_length_3626_cov_3.886866"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..692)
                     /locus_tag="PK348_04965"
     CDS             complement(<1..692)
                     /locus_tag="PK348_04965"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HXK65596.1"
                     /translation="MIKNLSEKTSKIKDIRLLILLVVLINIFFVTQIYSAEKPLIIVT
                     SEGFPPFSYIDKSGILRGMIIDFWNLWSAKTGIPVKIIHAPWKESINMIKEKRADIHS
                     GLFITKDRSMYMDFSKKFFTIKTSLFMRSNIDNPLNVILHDSSLKIGAQYDTGAYLFL
                     KEKFPWIHIKVYEDTARLIEAAANGEIDAFSEDEPSARYYLSNYHILGEFKIISILSE
                     EDIHAGVPKNNV"
     gene            1077..1754
                     /gene="pncA"
                     /locus_tag="PK348_04970"
     CDS             1077..1754
                     /gene="pncA"
                     /locus_tag="PK348_04970"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014587290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HXK65597.1"
                     /translation="MTQLHTINNYGTATITRHDALLVIDMQYDFIPGGSLPVAEGDTI
                     VKPIAHLMEQFFIKGATVVCTQDWHPSGHRSFASTHGKHPYDPINEPGIGPVLWPDHC
                     VIGTKGASLHDELPAHFCHAIVRKGYNINIDSYSAFLENDKKTATGLSGYFSNRGVKR
                     IFVCGLAFDYCVHFSAVDGASAGFEAVVITDCSKAVNSPATSVDDAIANMKAHNVSFC
                     SSADLSF"
     gene            complement(1746..3008)
                     /locus_tag="PK348_04975"
     CDS             complement(1746..3008)
                     /locus_tag="PK348_04975"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HXK65598.1"
                     /translation="MDKRDTISYVQGKGGEVVLTPEEIKTLDTINQKIASGQSLIDII
                     EYLFKETQHIMPCDRIGIAFSDDGKRLTLYHVVASYSPLYLDKGYTADLKGSSLETIF
                     SNETPRIINDLEEYYKNNPSSESTRLLLKEGVMSSMTCPLYVEGRIVGVLFRSSRKKN
                     MYSSREIALHQAIAERLSQAVEKAYRIEQLTQAINAYMEMLSFVTHELKSPLSSIVTL
                     GTTLKQGYFGEIPEHCTNTIDRILAQAEYLLTIVNEYLNLARLETGSMPVRKSTVTIA
                     HDIIEPALAIVQPQADAKHITVEKDYEDVQLKCDPDAMKIVMHNLLSNAIKYNSDNGI
                     VRITVKKEGTICKISVWNTGPGFPESEKHKLFKRFSRIETDELLARKGSGIGLFVSWN
                     IVYLHSGRIYANSVLGQYAEFTVELPLE"
BASE COUNT         1117 a          722 c          590 g         1197 t
ORIGIN      
        1 tttacattat ttttagggac ccctgcatga atatcctctt cggacaagat gcttattatt
       61 ttaaattcac caagaatatg ataatttgac agataatatc gagcacttgg ttcatcttcg
      121 gaaaaagcat caatttctcc atttgctgcc gcttcaatta gcctggcagt atcctcataa
      181 actttaatat gaatccatgg aaatttttct tttaagaata gatatgcacc ggtatcatat
      241 tgtgctccaa tttttagtga actatcatgt aaaattacat tgagaggatt atcaatattt
      301 gaacgcataa aaagggacgt tttgattgta aaaaattttt tgctgaaatc catatacata
      361 cttctatctt tcgtaatgaa taatccacta tgtatatctg ctcttttttc cttaatcatg
      421 ttaatacttt ctttccacgg agcatgaatt atttttacag gaattccggt ttttgccgac
      481 catagattcc aaaaatcaat aatcattcca cgcaaaattc cacttttatc aatataagaa
      541 aatggcggaa atccttcgga agttactata ataagaggct tctcagcact atatatctgt
      601 gtaacaaaaa aaatattaat tagaaccact aataatatta acaatctaat atcctttatt
      661 tttgatgttt tttctgataa attttttatc atttcacgaa tgcctctatt ctcattacaa
      721 cataggatag taattattta tagataaaat acttttatta caaagtttat tcttttgcaa
      781 atgattaaca aaacaaacta aaggagaaaa ccttcaacaa taatcataag aattagtaaa
      841 aatgtccact cataaacaac ttttaataat aattaataca caatgaaatc ttctctacca
      901 aaaaataaac aattttaaat tgagtaaaaa atattattga aataacaatc ttttgttcat
      961 aaatttttat tgaaacatag tatacactat aacactttga aagaaaatta tacagagcat
     1021 cctgaacaat aagagtgtta ctaacttaaa aaatatatcc acataggagg ctacctatga
     1081 cacaattaca tactatcaac aactatggta cagcaactat cacccgtcat gatgctttgc
     1141 tggttataga tatgcagtat gactttatcc ctggaggctc attaccagtt gccgaaggcg
     1201 acactatagt aaagcccata gcacacttaa tggaacaatt ctttataaaa ggtgcaaccg
     1261 ttgtatgcac acaggattgg caccccagcg gtcaccgttc atttgcatca actcatggaa
     1321 aacatcctta tgatcccatt aatgaacctg gtattggacc agtgctgtgg ccagatcact
     1381 gtgttatagg cacaaaaggg gcaagccttc atgacgagtt acctgcacac ttttgtcatg
     1441 caatagtgcg caaaggctac aacataaaca tcgatagcta ttcggcattt cttgaaaatg
     1501 ataaaaaaac agcaacgggg ctatcgggat acttttcaaa ccgaggcgta aaacgaatat
     1561 ttgtatgcgg acttgcgttt gactactgtg ttcattttag tgcagttgat ggcgccagtg
     1621 ctggatttga agctgtagtt atcaccgatt gttccaaggc agtcaattca ccagctacaa
     1681 gcgtggatga tgccattgct aatatgaaag cccacaatgt ctcattttgc tcatcagctg
     1741 acctttcatt ctaatggcaa ctcaacggta aactcagcat attgtcccaa cactgagttt
     1801 gcataaattc tgccactgtg caggtacaca atattccatg acacaaaaag cccaatgcct
     1861 gaccctttgc gggcaagcag ttcatcagtt tcaatgcgcg aaaaccgctt gaacagttta
     1921 tgtttctctg attcaggaaa ccccgggcca gtattccaca cgctgatttt gcatatagtg
     1981 ccttcttttt ttacggttat acgcacaatc ccgttatcac tattatactt gattgcattg
     2041 cttaagaggt tatgcatcac aatcttcatg gcgtcgggat cgcactttag ctgtacatcc
     2101 tcataatctt tttcaacggt tatatgtttt gcatcagcct ggggctgtac aatggcaagt
     2161 gcaggttcaa taatatcatg tgcgatagtt actgttgatt tgcgtactgg cattgaacca
     2221 gtctcaaggc gggctaagtt caaatactca tttactatcg ttaacagata ttctgcctgc
     2281 gcaagaattc gatcaatggt gtttgtgcaa tgctcaggga tttctccaaa gtatccctgc
     2341 tttagtgttg tacctaacgt tactatagat gataaagggc tttttagttc atgagttaca
     2401 aatgatagca tctccatgta agcgttgatt gcctgggtta actgctcaat gcggtatgct
     2461 ttttcaacag cctggctcag tcgctcagca atagcctgat gcagtgctat ctcacgtgaa
     2521 ctatacatat tctttttacg ggaactcctg aacaatacac ctacaatgcg cccttccaca
     2581 tatagtgggc aggtcatgct tgacataaca ccttctttca aaagcaggcg cgttgattca
     2641 cttgatggat tatttttgta atactcctct aaatcattaa taatacgcgg tgtttcattg
     2701 ctgaatattg tttcaagaga actcccctta aggtcagcag tatatccttt atccaaataa
     2761 agagggctgt atgaggctac aacgtggtac agcgttaagc gcttgccatc atcagaaaat
     2821 gcaataccaa ttctatcaca gggcatgata tgttgtgttt ctttaaataa atattctatg
     2881 atgtcaatga gtgattgccc tgaagctatc ttctgattaa tggtatctaa tgttttaatt
     2941 tcttctggtg ttaaaacaac ttctccacct ttaccctgta cataggaaat tgtatcacgt
     3001 ttgtccatgc atcattcctc attacattta ccatgaacaa gtttacagta tccatacaat
     3061 tatgcaaggt ttttatgtag tatcattttc attgtatttg taatttcatt tttcctatgt
     3121 ttttaatttt tcactaatca tttatattta atttcaaaca ttatctacca taattatcaa
     3181 aaaaagcatc acatcgtttt taatgttttt tacacaaaat cttttttatt ttcgtaaggg
     3241 ggtatttatc actaaatacc cccttactaa cccccaaagt gcgcctcaaa cggcggcgtg
     3301 ccttacttgc tatgtagtaa ttattccacg gcgggctgcg gaatagcatc cgttcaggtc
     3361 ctcgcccgca tcccaatttg ccgtacttcc tgtacggcaa aaccttgcac cgtttttatt
     3421 ataatattca aatcattact tcatattctc taaaatattg acaataaaag aaagccttgc
     3481 ttcagaatgt gctgtatgtg aggattagga aaaatttttc attagtaact atcgcacaaa
     3541 aaaactactc aattatttat acgtttgaag gtactggtgc ggtatccata tagtgctttg
     3601 ctatgatgag gcgttgtatc tcactt
//
LOCUS       DAOSZP010000180         3583 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_20863_length_3583_cov_3.311224, whole genome shotgun sequence.
ACCESSION   DAOSZP010000180 DAOSZP010000000
VERSION     DAOSZP010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3583
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20863_length_3583_cov_3.311224"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /gene="pth"
                     /locus_tag="PK348_04980"
     CDS             complement(<1..358)
                     /gene="pth"
                     /locus_tag="PK348_04980"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HXK65599.1"
                     /translation="MKIIACLGNPGKKYAGNRHNVGMIAGIEIAQKYSIVINKKEHDA
                     ITGNGSIEGYDCLFVLPQTYMNNSGIAVQKVLSYYKETPENLIVLHDEIELQFGHVKI
                     KFGGGHKGHNGIRSIQQ"
     gene            complement(355..693)
                     /locus_tag="PK348_04985"
     CDS             complement(355..693)
                     /locus_tag="PK348_04985"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HXK65600.1"
                     /translation="MKITRKSIDGLVIIKVEGSLVTEHIKQFEKEVYSALEKNNNIII
                     DFSELSFICSAGLSLLIASHKKAEPKNLKIVITGCSEDIIKLFKLTELDQHLLIKNTI
                     EEARLYISSQ"
     gene            1097..2050
                     /locus_tag="PK348_04990"
     CDS             1097..2050
                     /locus_tag="PK348_04990"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HXK65601.1"
                     /translation="MRAARIVAPRRFSVDNDPVPDCPDAHVRVKLLKAAICGSDFPYF
                     ANDYPKVSYPLPPGYPGHECLGVIEASRVKHIKEGTLVMYYPPFLDAYKEYHVTPPSR
                     IQEIPNTLPLNELLMTQLLGAVSHCAFRIDKPYNKHVVVMGQGPVGLFFTALMKNFGA
                     RSIIGVDILDYRLSVSKLMGATHTINPEKEDLIESVKTITGGSLADIVIDAYGQNVAV
                     INKCFEIAKHNGQVAFFGICLEEAPRLQFNTFFRKELRMIASVGPDLSMDYPYALDMI
                     VKGAIDVTPILTHDLPFEDIQKGFEMAIQRADNAIKIVLSF"
     gene            2083..2544
                     /locus_tag="PK348_04995"
     CDS             2083..2544
                     /locus_tag="PK348_04995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65602.1"
                     /translation="MPRAKIYGYVAVIVSALLGIAFVYYAFIHFDMYSSLHFSSIDTG
                     AYWAVVTPIAVVTLFILATGFWVGYTILTIKVAPPMPEIVEKKDNSKFKAFLLCLVTA
                     ALAAWFVYGLLHHYYLAIAIPAACITLVILGMVFWVGIAIITTRSTLKKDK"
     gene            2551..>3583
                     /locus_tag="PK348_05000"
     CDS             2551..>3583
                     /locus_tag="PK348_05000"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HXK65603.1"
                     /translation="MRALFLCYRGNPFCGGQGIYLYNLTRELAQLGVEIDVIVGPPYP
                     DPMDEWATVYRLENLNMWAIKTKHFPYEKLIKLYKPWHFTDYVLTRFHIFPEMETFSM
                     KAFFLLRKLLKEKHYDIIHDVQCLGWGLLPMKGYGIPIVTTVHHPLTKDREADFLVDN
                     TMWEMVCTILFYPLTMQRIVINRLDRIITSSKEGIKALYEAFKLPAEKVSVVYNGMDV
                     EVFRNTGEKRKENTILFVGNTEDHKKGLRYLFDAMTMLPEDVKLIIVDDGPPKRLTAW
                     GWIQERHLEHRVTFTGKVDLGTLVHLYSTCTMLVMASLYEGFGLPAAEAMACNTPVVV
                     TSAGSLPEVV"
BASE COUNT         1050 a          695 c          685 g         1153 t
ORIGIN      
        1 gctgctgaat agaacgaatg ccgttatgcc ctttatggcc tccaccaaat tttattttta
       61 catgcccaaa ctgaagttct atttcatcgt gcagaacaat gaggttttca ggggtttctt
      121 tataatatga cagcaccttt tgcactgcaa tgccgctgtt attcatatag gtttgaggca
      181 gcacaaacaa acaatcatac ccttcaatac taccgtttcc cgtgatggca tcatgctctt
      241 ttttgtttat tacaatggaa tacttttggg caatttcaat gccagcaatc atgccaacat
      301 tatggcggtt accagcgtat ttttttccgg gatttcctaa gcaggctatt atcttcattg
      361 tgaggatata tataagcggg cttcttcgat agtattttta atcaataaat gctgatccag
      421 ttcagttaat ttaaataact ttataatatc ttcactgcat ccagtaataa ctatttttaa
      481 gttctttggt tctgcttttt tatgggatgc aataagcagg cttaaacccg cactgcatat
      541 aaatgataat tcgctgaaat caatgataat attattgttt ttttcaagtg cactatatac
      601 ttctttttca aactgtttaa tatgttcagt aacaagagaa ccttcaacct ttatgataac
      661 aaggccatca atgctctttc tggtaatttt cattgcacca tcaacccctt gaattatcta
      721 ttagcagtaa atatataggc atttatccta taatttccct tataattgtc aagcaaaaga
      781 tatttgaagc tacatactaa aatttataat tttgctgtta ctcatgtata agtataagcc
      841 ttttattaat aaattcaaag attattttca accaaaatgc tattactatt aactatggga
      901 caatacatga cattattatg ggtgattacc tgcccaactg accacccacg gcattgtcta
      961 aataatttgc tttaactttg catatacata ataaaatgtc aaataaataa gttgacaata
     1021 accccactct ataacactgt gtagttctta attatttgta tatattaatt tactctattt
     1081 acaggaaatt ttgaccatgc gcgctgcacg aatagttgct ccccggaggt tttccgttga
     1141 taatgaccca gtccctgatt gccctgatgc tcatgtcagg gttaaacttt taaaagctgc
     1201 tatctgcggt tcagattttc catactttgc aaatgattat ccaaaagtat cttatccact
     1261 acctcccggt tatcccggtc atgaatgtct gggagttatt gaagcatcaa gagttaagca
     1321 tataaaagaa ggcactctgg taatgtatta tccacctttt cttgatgcat acaaagaata
     1381 ccatgtcact ccaccttcac gaatacagga aatacccaat acattgccct taaatgagct
     1441 tttaatgaca cagcttctgg gtgcggtttc acactgtgca ttcagaatag acaaaccata
     1501 caataaacat gttgttgtaa tgggacaggg acctgttggt ttgttcttta ctgcacttat
     1561 gaaaaacttt ggtgcccgca gtattatagg tgttgatatt ctggattatc ggttgagtgt
     1621 ttcaaaacta atgggagcca ctcacaccat taaccctgaa aaggaagatt taattgaatc
     1681 agtaaagaca attaccggtg gttcactggc tgatattgtt attgacgcat acgggcagaa
     1741 tgtggcagtt attaataaat gctttgagat agcaaaacat aatgggcagg ttgctttttt
     1801 tggtatttgt ttagaagaag cgccacggct gcaattcaat acatttttca gaaaagaact
     1861 gcgcatgata gcttctgttg gccctgatct ttccatggat tatccatacg cgctggatat
     1921 gattgttaaa ggagctatcg acgtaactcc aattttgaca cacgacctgc cttttgaaga
     1981 tattcaaaaa ggttttgaaa tggcaataca acgagctgac aatgcaataa agattgtatt
     2041 aagcttttaa atggtatact gttaacaaaa agaggttata ttatgccacg agcaaaaata
     2101 tacggatatg tagcagttat tgtttcagcg ctgctgggaa ttgcatttgt atattatgca
     2161 tttatacatt ttgacatgta ttcatcgtta catttttctt ctattgatac cggtgcatac
     2221 tgggctgtag ttacgccaat tgccgtcgtt acccttttta ttttagcaac cggtttttgg
     2281 gttggatata caattttaac gataaaggtg gctcctccta tgccagaaat agttgaaaag
     2341 aaggacaatt caaaatttaa ggcattcttg ctttgcctgg ttaccgctgc tcttgcagcg
     2401 tggtttgtat atgggttgtt gcatcattac tatttagcta ttgctattcc tgctgcatgc
     2461 ataacactgg ttatcctggg catggtgttc tgggttggca ttgcaataat tacaacacgt
     2521 tcaactctga agaaagataa atagccattt atgcgtgcac tatttttgtg ttaccgtgga
     2581 aacccgttct gtggcggaca gggcatttat ttatacaatc tcaccagaga actggcgcaa
     2641 ctgggtgttg agattgatgt cattgttggc ccaccatacc ctgaccccat ggatgaatgg
     2701 gctacggtat acagacttga aaatcttaat atgtgggcaa taaaaacaaa acatttcccc
     2761 tatgaaaagc ttatcaaact gtataagcca tggcatttta cggattatgt attaacccgt
     2821 tttcatattt ttccagaaat ggaaacattc agcatgaagg ctttttttct tttgcgaaag
     2881 ctcctgaaag aaaaacatta cgatattatc cacgatgtgc agtgtttagg ctggggactt
     2941 ttacctatga aaggctacgg cataccgata gttactaccg tacatcatcc actgacaaaa
     3001 gaccgtgaag ctgatttcct tgttgataac accatgtggg aaatggtgtg taccatactg
     3061 ttttacccct taaccatgca acggatagta attaaccgcc ttgataggat tattacctct
     3121 tccaaagaag gtatcaaagc attgtatgag gcatttaagc taccagctga aaaggtttca
     3181 gtagtctata atggcatgga cgttgaagta tttcgcaata ccggtgaaaa gcgtaaagag
     3241 aacacaatcc tttttgtggg caacactgaa gaccataaaa agggattgcg ctaccttttt
     3301 gatgccatga ccatgctccc tgaagacgtt aagctcatca tagttgatga tggaccacca
     3361 aaacggctca ccgcctgggg ctggatacag gagcgtcatc ttgaacacag ggtaacgttt
     3421 acgggtaaag ttgatttagg cacactggta catctgtaca gcacctgcac catgttagtc
     3481 atggcttcac tgtacgaagg gtttgggctg cccgcagccg aggcaatggc atgtaataca
     3541 ccggtagttg taaccagtgc cggttcattg cccgaggtag ttg
//
LOCUS       DAOSZP010000181         3580 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_20882_length_3580_cov_3.567943, whole genome shotgun sequence.
ACCESSION   DAOSZP010000181 DAOSZP010000000
VERSION     DAOSZP010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3580
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20882_length_3580_cov_3.567943"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(134..1222)
                     /locus_tag="PK348_05005"
     CDS             complement(134..1222)
                     /locus_tag="PK348_05005"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HXK65604.1"
                     /translation="MKRILIYSLILGLAVLGNSCTKQQIDDYASIAFSIGEVKKNGQP
                     VNIGDILNQNDIIETGTMSSCDIKIGDSMIRIKENSKAILAQLLRKDGLENTTLGLDV
                     GKMICKPKKLLKNESFLVKTPTAVAGVRGTNFSVEADAQKTTRIKVFDGKVAVVKRVE
                     AVEEHIDKIIEAAPAVEEKEKVVITTEDVKKAEKKIEEVMKKEGEATPEAIEKVVAAA
                     KEEVVVKKEEVQKFKPEDFKEEKQEIIKIEEKPKEVVKEIAKVVKKTRQIPQPEGQLL
                     VTRYEIYFVKNGRVEWEGKVINPPTKTEDKIYIASGDYIFCAKLDGTVLWRKKLANDG
                     KVELEGEKVAVYAGGQKKLLDKLTGEEE"
     gene            1523..2728
                     /locus_tag="PK348_05010"
     CDS             1523..2728
                     /locus_tag="PK348_05010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65605.1"
                     /translation="MDRSKLIQILHGIIIADALASPLDTLSAEHIHNVFGTIHDYTDP
                     APALKNKLYLWKKPALYTALSQYCILLSALNAQQDTYVHQHACNFISSLGDSSRIFRH
                     PQGFLQHLQSPYDAPTAELLICIPALFFLHKQNPISIIHFILSHNKNAATCAASVFVY
                     ILLETIIMRQLIALDSVVLKQTAEDAYQFVQEHSALFFNNGGNPQNIIEATKDLHDLI
                     INLPLESDETHYTRYCIPFANRWSKNEYTRLTVNHPFTLLPLALYCVHTGQKDSLLYS
                     AIHKGGKISLLTPLVALLATAMYGYEVIPQNLMDTIVNKKRILQLLALLQSHSISINY
                     LTDFFQNEAKLTQKELEERESKLKHFKPKQTGKNKDKDKYASMNYHIVESWTKLDKAK
                     WKKERRRKK"
     gene            complement(2787..>3580)
                     /locus_tag="PK348_05015"
     CDS             complement(2787..>3580)
                     /locus_tag="PK348_05015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65606.1"
                     /translation="KHEGWPGIPHGGLAMTTFIELIDIAKEFPAIFPYEYRFRFAGER
                     LMCGDVVRCIVQKGDARYFFGSFGKSLQQPYCNCRYTEGQAHEYGNGVIEILKNNMDA
                     STPFIIPNMSTKLTSTVTTDHDQRIFYITDYTKDRTYLILHLTGKNNFAQAPDGSVHN
                     GVLSSMLDETMGWSTFLSVWQAGVTVELQITFYEKVYNTDNFHIIGFCDDIRGEYGKK
                     ITTAYGGVVAQRGEELSIIALAYGRWLTLPGFKELMVQYLKHYIE"
BASE COUNT         1080 a          780 c          541 g         1179 t
ORIGIN      
        1 gctgatattg ccaaacttat tgaaaaagca agccagcagt ttgacttaac tccactgcaa
       61 acagaatttt tagtaaataa atttatctta aataaataat aaaaagggct tgctttcgca
      121 agcccttttt atgttattct tcttcacctg tcagtttatc taatagcttt ttctgaccac
      181 ctgcatatac tgctaccttt tcgccttcta attcaacctt gccatcattt gccagtttct
      241 ttctccaaag cactgtacca tctaattttg cgcagaagat ataatcacca gaagctatgt
      301 aaattttatc ctcagttttt gtgggagggt taatgacttt accttcccat tcaactcgac
      361 cattctttac aaaatatatt tcgtagcgtg ttacaagcaa ctggccttca ggctgaggaa
      421 tctgccgtgt tttcttaaca acctttgcaa tttcttttac cacttctttt ggtttttctt
      481 caatttttat tatttcctgc ttttcttctt taaaatcttc aggtttaaac ttctgcactt
      541 cctctttttt taccaccact tcttcttttg cagcagctac aactttttct atggcctctg
      601 gggtggcttc accttctttt ttcattactt cttctatctt cttttcagct tttttcacat
      661 cctcagtagt tatcactact ttttcctttt cttcaacagc aggtgctgcc tctattattt
      721 tatcaatatg ttcctcaacc gcttcaactc gctttactac agctaccttc ccgtcaaaga
      781 ctttaatgcg tgtggttttc tgggcatcag cttccactga aaagtttgtc ccacggacac
      841 cagcaactgc agttggcgtt tttactaaaa agctttcatt ctttaacagc tttttgggct
      901 tacagatcat cttgccaaca tcaagaccca atgtagtatt ttccagccca tcttttcgta
      961 aaagctgtgc cagtattgcc tttgaatttt ctttaatgcg gatcatggaa tcgccaatct
     1021 taatatcaca ggaagacatg gtgcccgttt caatgatatc gttctgattt aaaatatcac
     1081 caatgttgac aggctgacca ttcttcttta cttcgcctat gctgaatgca atggaagcat
     1141 agtcatcaat ctgttgtttg gtgcagctat ttcccaaaac tgcaagacct agtatgagtg
     1201 aatatatcag aatccgtttc atcacgcccc cctataataa aaaaattata taccagatta
     1261 tgtaatacct tgcttaaaaa ataaccattt aaccattata gtatactatt gtttcataaa
     1321 taaatgcaat ttttttttat tatacctgaa aattaatact ttagtaacaa aaagatataa
     1381 atcatacaat aaattaatta ttctatacca atattggtga cactggtata gacatgacat
     1441 ttcacgtcta tatatgcact ttgctgtatt aatatatatt ttcatacttg actgttacca
     1501 gcaggtatac tgttctatca ccatggatcg ttctaagctc atacaaatcc tgcacggcat
     1561 tattattgcc gatgctcttg caagtcctct tgataccttg agtgctgagc atatacacaa
     1621 tgtttttggc acaatacacg attatactga cccagcgcct gcacttaaaa ataagctcta
     1681 tctgtggaaa aagccagcac tctacactgc cctttcacag tattgtattc ttctttcagc
     1741 actcaatgct caacaagata cctatgtgca ccagcacgcc tgtaacttta tatcttcttt
     1801 aggcgatagt tcccgtatat ttcgtcatcc gcagggattt ttacaacacc tgcaatcacc
     1861 gtatgatgcc cccactgccg aattgcttat ctgtatccct gctctattct ttttacataa
     1921 acaaaaccct atctcaatta tacattttat tctcagtcat aacaagaatg cagctacatg
     1981 tgcagcatca gtatttgtgt atatattgct tgaaacaatt ataatgcgac agctcatagc
     2041 attggatagt gttgtattaa aacaaactgc agaagatgcg tatcaatttg ttcaggaaca
     2101 ctcagcgctt ttttttaaca acggtggcaa tccacagaat atcattgaag ctacaaaaga
     2161 tcttcatgat ttaatcatca acttgccact tgaaagtgat gaaacacact acaccagata
     2221 ttgtatcccc tttgcaaaca ggtggtcaaa aaacgaatat acccggctta ccgttaatca
     2281 tccttttaca ttgttgccat tagctctgta ctgtgttcat actggtcaaa aagactcttt
     2341 actctattct gccatccata aaggcggcaa gatatcgtta ttgactccac tggtggcttt
     2401 gcttgcaaca gccatgtatg gttacgaggt aattcctcaa aacctaatgg acaccattgt
     2461 caataaaaaa agaattttgc agttgttagc tttattacag agtcattcca tttctataaa
     2521 ctatctcaca gatttttttc aaaatgaagc aaaattaaca caaaaagagt tagaagagcg
     2581 tgaaagcaaa ttaaaacatt ttaaacccaa acaaacaggg aaaaataaag acaaggacaa
     2641 atatgcttca atgaactacc atattgttga aagctggaca aaattagaca aagcaaaatg
     2701 gaagaaggaa agacgaagaa aaaaataata tattttacct ctgctttctg caatagaaag
     2761 aaccaataat gaatattgta ctgattttat tcaatataat gttttaaata ctgtaccatg
     2821 agctctttga aacctggcaa tgtcagccat cgtccgtatg ctaaggcaat gatggatagc
     2881 tcttcaccac gctgtgccac cacgccacca taggcggtag taatcttttt gccatattca
     2941 ccgcgtatat catcacaaaa tccaattata tggaaattat cagtattgta tactttttca
     3001 taaaaggtta tctgcaattc aacagtaaca cctgcctgcc ataccgaaag gaatgttgac
     3061 catcccatag tttcatccag cattgaactg agcacaccgt tgtgcacaga gccatcagga
     3121 gcctgtgcaa aattattttt cccggttaag tgtaaaatga ggtaagttct gtcttttgta
     3181 tagtcagtta tataaaatat tctttgatcg tgatctgtag taacagtact ggttaactta
     3241 gtagacatat tgggaataat aaacggagtt gaagcatcca tgttgttttt cagtatttca
     3301 attactccat taccatactc atgtgcctgc ccttcggtat aacgacagtt acaatatggc
     3361 tgctgcaaag attttccaaa tgagccaaaa aaatatcgag catcgccttt ctggacaata
     3421 caccgtacca catcaccaca catcaaccgc tcaccagcaa aacggaatcg gtattcataa
     3481 ggaaatatcg caggaaattc ctttgcaata tcaatcagtt caatgaatgt ggtcattgct
     3541 aagccgccat gaggtatacc aggccaccct tcatgttttt
//
LOCUS       DAOSZP010000182         3570 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_20944_length_3570_cov_3.264011, whole genome shotgun sequence.
ACCESSION   DAOSZP010000182 DAOSZP010000000
VERSION     DAOSZP010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3570
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20944_length_3570_cov_3.264011"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2409
                     /locus_tag="PK348_05020"
     CDS             <1..2409
                     /locus_tag="PK348_05020"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HXK65607.1"
                     /translation="GYFTVNVSNQPATFTTLAPKFLYQHAKLYIADTHSAVVTSANLT
                     YSGLVQSREAGIVVTNEDDVLFFVEKFDEYFSQAQPLAQELLEKLQELLALYPPYYIY
                     MRSLLLLYDMPEDELPDKLPPLAGYQKPIVSRLLRIIEEHSGAWLIASTGLGKTVMAA
                     HIAAYLCMNKRINAALVLCPAGLKRMWHRILRAARVSSEEFSYYILSLDDFKKSIDVA
                     HLLSELAHIDENTLIILDESHHLRNIGSAENIRLSHQRIMDAVHERKAKILLMTATPY
                     STGVDDINSQLVLLPHKEVDGLFGKCVQPWCIESPSELSELLPVVVLTAPTVVKYYSS
                     IDEAGNRFVLFLNNEKRYFPHKLHFQNIEYTNQGDSILHQLLQSKLLYRKVNDTSNTL
                     FHDTAGVRDPLWEAQIVHQACSSLKQLDELLDKLSREDGFDKIRFHNQERLSSFAVDS
                     RVRLQPLLYERDEKIQKLANLIERFNHEKVVVFCHYRETARYIKDALQRILPHVVVES
                     TVDADTNKIESIIQRFAPIANKADYVDDENSVNGKEIQVLVATGAMSEGFNFQDARIL
                     VNFDLPWTVLTLAQRMGRILRPWFEPRDVYVFNFIPSTMGSDIVYHASRWKNRLLTRN
                     KEISSFADIPVLVADGGKDFEMINLAKSIALFGDHDLNLDEVLEFIETADNLHTSGFI
                     DDIAQLDESVKREVLRLPSGIRSIKKSNLKEPIVIYILFKCKNRYYPVLFDGKEKILY
                     DSEHMDEIMHIIRSLKDEEPFYNAEIVQSIDSVLVKAKNEWVVKHQFHPYEVMIDCYC
                     VLLS"
     gene            complement(2406..>3570)
                     /locus_tag="PK348_05025"
     CDS             complement(2406..>3570)
                     /locus_tag="PK348_05025"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HXK65608.1"
                     /translation="WVALKALGACTKSVFYKSPIGTLKLVEIPEPEPINNNWAIVKTT
                     YCGFCGSDFNLILLHDSPMASPFTSFPCVIGHEVCGTIEEPGNTGFKKGQRVVINPML
                     SCAVRQLPLCASCKKGRPANCENFANGTISPGMFTGICKDVNGGFAEYLPAHRAQLFA
                     IPGSISDKAAVLTEPLSVALQAIYDNMPQHSDTVCIIGCGVIGLMLVRALRALNCKAT
                     IAVIEPSEFHRQKALSLGANAIVQGDLLEHAATFTSGKVYTPMFGPKILQGGFDRVFD
                     TVGSSKTFSLAINIARAGGVVSLVGITSKLSYDPTPLWLKLLTVKGVYGYGFIREGSK
                     TKHIFAKALELMKKMPDIETMVTHTFSIDQYKEMIEVNSNKNAHKAIKTAITF"
BASE COUNT         1071 a          711 c          744 g         1044 t
ORIGIN      
        1 ggctacttta cggtaaacgt aagcaatcaa ccagcaacat tcacaacact agcgccaaaa
       61 tttttgtatc aacatgccaa gctctatatt gctgacacgc atagtgcagt ggtaacatcc
      121 gcaaacctta cctacagcgg tttggtgcaa agcagggaag cagggattgt tgttaccaat
      181 gaggatgatg tgcttttctt tgtagaaaag tttgatgaat attttagtca ggctcaacct
      241 ttagcccagg agcttttaga aaaattacag gaattgcttg ctctctatcc gccatactat
      301 atttacatgc gctcattgct acttttgtat gatatgcctg aggatgagtt acctgacaaa
      361 ttaccaccac ttgcgggata tcaaaaacct attgtatcgc ggctactaag aataattgaa
      421 gaacacagcg gtgcctggct tattgcttca acaggccttg gcaaaacggt tatggcggcg
      481 catattgcag catatctttg catgaataaa cgtataaatg ctgcattggt actatgtcca
      541 gcggggctca agcgtatgtg gcacaggata ctgcgtgctg cacgagtgtc aagcgaggag
      601 ttttcatatt atattctatc gcttgatgat ttcaaaaaat caattgatgt ggcacacctt
      661 ttaagtgagt tagcacacat tgatgaaaat acgctgataa tccttgatga aagccatcat
      721 cttagaaaca ttggttccgc agaaaacatt cgcctttcac atcagcgtat catggatgct
      781 gtgcatgagc gcaaagcaaa aatactttta atgacagcaa caccatacag cacgggtgtt
      841 gatgatatca attcacagtt agttctgttg ccacacaaag aagtagatgg tttgtttggt
      901 aaatgtgttc aaccatggtg catagaaagc ccatcggagc tatcagagct actgcccgtt
      961 gttgtattaa ctgcaccaac agttgttaaa tactatagct ctatagatga ggcaggaaat
     1021 cgatttgtgc tcttcttaaa taatgaaaag cgctatttcc cccataagct gcatttccag
     1081 aatattgaat ataccaatca gggcgatagt atcctccatc agttattaca aagcaagctt
     1141 ctgtatagaa aagtaaatga taccagtaac acgctctttc atgatactgc aggtgtacgc
     1201 gatccattgt gggaagctca gattgttcac caggcgtgtt catcgttaaa acaattagat
     1261 gagttgcttg acaagctttc gcgtgaagat ggctttgata aaatacgatt tcataatcaa
     1321 gaaagattga gctcatttgc tgttgatagt agagtgcgcc tacaaccatt gctgtatgaa
     1381 cgtgatgaaa agatacaaaa attagctaac ctgattgagc ggtttaacca tgaaaaggtt
     1441 gtggtatttt gccattatcg agaaacagca cgctacataa aagatgcatt gcaacgaata
     1501 ctgcctcacg ttgttgttga atctacggtt gatgctgata ccaataaaat tgaatccatt
     1561 atacagcggt ttgcaccaat agcaaacaaa gcggattatg tagatgacga aaacagtgtc
     1621 aatggaaaag aaatacaggt gcttgtggca acaggtgcaa tgtctgaagg cttcaatttt
     1681 caggatgcgc ggattctggt aaactttgat ttgccatgga cggtactcac gcttgcccaa
     1741 cgcatggggc gaattttgcg tccatggttt gaaccacgtg atgtatatgt gtttaatttt
     1801 attccatcaa caatgggcag tgacattgtg tatcacgcat cgcgatggaa aaaccgcctg
     1861 ttaacgcgca ataaagagat aagctcattt gcagatatac ctgtacttgt tgccgatggc
     1921 ggaaaagatt ttgaaatgat caatctggca aaatcaatag cattgtttgg tgatcacgat
     1981 ctcaatttag acgaagtatt ggaatttatt gaaactgctg acaacctcca tacgtcaggc
     2041 tttattgatg atatagcaca gcttgatgag tctgttaaac gtgaagtttt acgcctgcct
     2101 tcaggaatac gcagtataaa aaaatcaaac ctgaaagaac ctattgtgat atacattctt
     2161 tttaaatgta aaaatagata ttatcctgtt ctttttgatg gcaaagaaaa gatactctat
     2221 gatagtgaac acatggatga aattatgcac attatacgtt cgctcaaaga tgaagagcca
     2281 ttctacaatg cagaaatagt tcaatcaatt gattctgttt tggtaaaagc taaaaatgaa
     2341 tgggttgtaa aacatcaatt ccatccatat gaagttatga ttgattgcta ctgtgtgttg
     2401 ctttcttaaa atgtaattgc tgtttttatt gctttatggg catttttgtt gctattaacc
     2461 tcaatcattt ccttatactg gtcaatactg aatgtgtggg taaccattgt ttcaatatcg
     2521 ggcatcttct tcatcaattc caatgcttta gcaaagatat gttttgtttt gctgccttcc
     2581 cttataaagc catagccata aacacctttg acggttaaca gcttaagcca taggggagtt
     2641 ggatcgtatg aaagcttgct tgttatgcca acaaggctta ctacaccacc agcccgtgca
     2701 atgttgattg caagtgaaaa tgtttttgat gaaccaactg tatcaaatac cctgtcaaat
     2761 ccaccctgca gtatctttgg cccaaacatg ggggtatata ctttcccact ggtaaatgta
     2821 gctgcatgct ctaacaaatc accctgtacg atagcattgg cacccaagct tagagctttt
     2881 tgcctgtgaa attcagatgg ttcaattact gctattgttg ctttgcagtt taacgcacgc
     2941 aatgcacgaa cgagcataag cccaataaca ccacagccaa taatgcacac cgtatcggaa
     3001 tgttgtggca tgttatcata gattgcctgt aatgctacac ttaatggctc agtgagcact
     3061 gctgccttat cgcttatgct accaggaatt gcaaaaagct gtgcacggtg tgccggcagg
     3121 tattctgcaa agccaccatt gacatccttg caaataccgg taaacattcc tggtgaaata
     3181 gtaccattgg caaaattttc acagttagca gggcgccctt ttttacagga agcgcatagc
     3241 ggtaattgtc gcacagcaca tgaaagcatg ggatttataa caacacgctg tcctttttta
     3301 aaaccggtat tgcccggctc ttcaatggtg ccgcaaacct cgtgcccaat cacacaggga
     3361 aatgaggtaa acggagaagc catgggcgag tcatgaagca aaatgagatt gaagtcgctt
     3421 ccgcaaaagc cacaatacgt tgtttttaca attgcccagt tgttgttaat aggttctggc
     3481 tctggtattt caaccagttt gagagtgcca atggggcttt tgtaaaatac agattttgta
     3541 catgctccca gcgctttgag cgctacccat
//
LOCUS       DAOSZP010000183         3567 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_20965_length_3567_cov_3.577449, whole genome shotgun sequence.
ACCESSION   DAOSZP010000183 DAOSZP010000000
VERSION     DAOSZP010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3567
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20965_length_3567_cov_3.577449"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(176..2266)
                     /gene="rnr"
                     /locus_tag="PK348_05030"
     CDS             complement(176..2266)
                     /gene="rnr"
                     /locus_tag="PK348_05030"
                     /inference="COORDINATES: protein motif:HMM:TIGR02063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HXK65609.1"
                     /translation="MSKTSSRIVQAIEKQKGFFTPNDIVSAVLLSTSKSKKSKKSTKV
                     SQRQAQQTANRVQNAISTLYQCGFLTKQKGKYRVAQPFSFTGTFIHSKKGDGIVYLHD
                     DIEVHIYREDVNHARNKDIVQVQLTDIRRGTLFGRVTSIVTANKQMFFARCVKPNGKF
                     RILQLIDVPGNIQVITKENIKPGVLAIVTVKNSFIQNYQECTINALIEHEEEYDVQRI
                     VGKFSLPGAHPEYEHLENIEQNVHPHELKNRKDYRKLLTVTIDGETAKDFDDAISIEY
                     KNGVYTLYVHIADVSAYVYKDDAIDKEAYNRGTSYYLGNTVIPMLPEKLSNDLCSLKA
                     GVDRLTLSVMLQFDKHGNYRTHEIYRGIINVDQRLTYNVAQEILNSKKRSNIKAMLGH
                     AKDLAQKLKAKRLSNGRVDLNLADAEMIFDNNASIADIAFATRLNSHMIIEEFMLSAN
                     EVVSRVLRQNNVPTLYRIHEPIAQEKFIALQNFLKTLGIILKEEENVGLALQRVIDSV
                     AQKEYQQVVNFIVLKSLMQAYYGPTPLGHFGLGFKDYTHFTSPIRRYPDLIVHRCIKS
                     LIDRVQPPYSPDELAVIGEQSSKLERLAQSAERDLLKLKACRLLEERVGEEFEGIISG
                     VTRFGFYVSLLDKPIEGMVPLKFLTDDYYLVNEDDYTVIGRRLGRRFRLGDLVKVRLI
                     NVDTDFMRIDFEVV"
     gene            complement(2285..2599)
                     /locus_tag="PK348_05035"
     CDS             complement(2285..2599)
                     /locus_tag="PK348_05035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65610.1"
                     /translation="MGILKDAGESLVNFSERFLDKTEELAQIARITMEIKKLEHSIKE
                     IYLNTGKYVYDQVVSDRPISNMDDFIIKAVATINDYKTKIQEKQNEIQKVKEHYESKY
                     HR"
     gene            complement(2778..>3567)
                     /locus_tag="PK348_05040"
     CDS             complement(2778..>3567)
                     /locus_tag="PK348_05040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65611.1"
                     /translation="LKLGLSAGVGYFQGKGGANISAYLDPGGTDQSEIAMIIDNELTA
                     LTAFTGTGLQYALTPHHSIGVFYHSPIYRLHASREERTTSYQVGPIFPTNSSETTINN
                     DDFRFRIIPAYISFGYGYISEGKWSCTVEGVVHNTNTDYPNRVINGKVGLEIYLLEDL
                     IVRTGFFTDFSQKDTVTQSSENDEKNDYYGATLSLSFGNDLNPLQSDTLKAKNMWTTV
                     GIVYQLGIGDIRGFRFYTVTSGSDPVIHQYTHRISLYIGESIVF"
BASE COUNT         1012 a          689 c          643 g         1223 t
ORIGIN      
        1 gtattgggtt tagctttaaa ggttttagta ttgataattg caaatacatc agataattgc
       61 tgcgggatga acgaattggt gtcaatctgt tcctgagtgc atagtattgc aacagtatcc
      121 gatgaagata ttgtttcaaa tctttttgcc tgtaatgtta gcatgactgt accttttaca
      181 ctacttcaaa atctatacgc ataaaatcag tatcaacatt tataagacgt actttaacaa
      241 ggtcaccaag cctgaagcgc cttcccagtc gcctgcctat tactgtataa tcatcttcat
      301 taaccagata ataatcatcg gtcaggaatt tcaaaggaac cataccttct ataggtttgt
      361 caagcaaact aacataaaac ccaaatcgtg taacaccgct tatgatacct tcaaattctt
      421 caccaacccg ttcttcaagc aatctgcatg ctttgagttt caaaaggtcg cgttcagcag
      481 attgggccaa acgttctaat tttgatgatt gctctccaat tactgctaat tcatcagggg
      541 aatagggcgg ttgtactcta tctattaatg attttatgca tctgtgcaca atcaggtcag
      601 gatagcgtcg tataggggaa gtaaagtgtg tataatcctt aaagcccaat ccaaagtgcc
      661 ctaacggagt aggtccgtaa tatgcctgca tcagtgattt gagaacaata aaattaacta
      721 cctgctgata ctctttttga gcaactgaat caataactct ttgcagagca agccctacat
      781 tttcttcttc cttaagaatt atgcccagtg tcttaaggaa attctgcagt gcaataaatt
      841 tttcctgggc aattggttca tgaatgcggt acagggttgg cacattgttt tggcgcaaaa
      901 cacgtgatac aacttcgttg gcgcttagca taaactcttc aataatcatg tggctgttaa
      961 gtcgtgtggc aaaagcaata tcagcaatag aagcgttgtt gtcaaatatc atctcggcat
     1021 cagctaagtt caggtcaact cttccgttgg ataatcgctt tgctttaagt ttttgtgcca
     1081 ggtcttttgc atgtcccagc attgctttta tattactccg tttttttgag ttcaatattt
     1141 cctgagccac gttgtacgtt aagcgctggt caacgtttat gattccacgg taaatttcat
     1201 gtgttcggta gttgccgtgt ttatcaaact gcagcataac cgaaagcgtt aacctgtcaa
     1261 caccggcttt gagtgagcac aggtcgttgg ataacttctc aggtagcatt ggtattacgg
     1321 tattgcctaa gtaatagctt gttccacggt tgtatgcttc tttatcaata gcatcatctt
     1381 tatatacata agcagacaca tcggctatgt gaacgtataa cgtataaaca ccatttttat
     1441 attctattga aatggcatca tcaaaatctt ttgctgtttc tccatcaatg gttactgtta
     1501 aaagctttcg gtaatctttg cgatttttca gttcgtgtgg atgtacattc tgttcaatat
     1561 tttcaaggtg ttcatattct ggatgtgcac caggcaaaga aaacttcccc acaatgcgct
     1621 gtacgtcata ttcttcttcg tgttctatta atgcattgat ggtgcattcc tgataatttt
     1681 ggataaaaga gttttttaca gtaactatcg ccagaacccc tggttttata ttttcttttg
     1741 taatgacctg aatattacct ggaacatcaa taagctgcag aatacgaaat ttgccgtttg
     1801 gttttacaca tcgtgcaaaa aacatctgtt tgttggctgt cacaattgac gtaaccctgc
     1861 caaagagcgt tccacgacgt atatcagtta actgtacctg aacgatatct ttgttgcggg
     1921 catgattgac atcttcacgg taaatgtgta cttcaatgtc atcatgcaaa tacacaatcc
     1981 cgtcaccttt tttgctatga ataaatgttc ccgtgaagct gaatggctgt gctaccctgt
     2041 atttgccctt ttgtttggtc agaaatccac actggtatag tgtggaaata gcattctgca
     2101 cacggttagc tgtttgctga gcctgacgtt gtgatacttt agtgcttttc tttgattttt
     2161 tactttttga tgtggaaagc aatactgctg acacaatatc gtttggagta aaaaaacctt
     2221 tttgtttttc aatagcctgt acaatgcgcg atgatgtttt tgacatagta tattttagct
     2281 tttactatcg atggtatttg gattcatagt gttctttaac tttttgtatt tcgttctgtt
     2341 tttcctgtat ttttgtttta tagtcattta ttgtggcgac agcttttatg ataaaatcat
     2401 ccatatttga aataggcctg tcacttacaa cctggtcata cacatacttt ccagtattca
     2461 agtatatctc ttttatggaa tgttccaact tttttatttc catggtaatg cgtgcaattt
     2521 gagccagctc ttcagtttta tctaaaaacc gttcgctaaa atttaccagt gattcacctg
     2581 catctttaag aattcccata acatacctca aatgataatg tgatagttgt tatactattt
     2641 gtatcagata gttacttatt actaaaaaag gattcctata atgaaaattc cttaaaaatg
     2701 ttatgcctat atggtggtat tcgtaataat aaactgaagt ttactataaa caatatagtt
     2761 attaaagtca atacctttta aaagacaatt gattcaccaa tatacaggct tatacggtgg
     2821 gtatattggt gtattacagg atcagagccc gaagtaacgg tataaaatct gaaaccacga
     2881 atatcaccaa tccctaactg gtatactatt ccaactgttg tccacatatt tttggctttc
     2941 agggtgtcac tttgcaacgg attaagatca ttgccaaacg aaaggctcaa cgttgcacca
     3001 tagtaatcat ttttttcgtc attttctgaa gattgggtta ctgtatcttt ctggctaaaa
     3061 tcggtaaaaa agcccgttcg tacaataaga tcttcaagta aatatatctc caacccaact
     3121 ttcccattta taactctatt gggataatct gtatttgtat tgtgaacaac accttcaacc
     3181 gtacatgacc acttgccttc actgatgtat ccgtatccaa atgaaatata tgctggaatt
     3241 attctaaaac gaaagtcatc attatttatt gtggtttcag agctatttgt tgggaaaatt
     3301 ggcccaactt gataacttgt agttctttct tcccttgatg catgcaatct atatatggga
     3361 gaatggtaga aaacacctat gctgtgatgg ggggtcagag catattgcag gccagtaccg
     3421 gtaaatgcag taagtgctgt aagttcatta tcaataatca ttgcaatttc actttgatct
     3481 gttccaccgg gatcaaggta tgcagatata tttgccccac ctttaccttg aaagtaacca
     3541 acacctgccg ataaaccaag tttcagg
//
LOCUS       DAOSZP010000184         3560 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_21017_length_3560_cov_3.170613, whole genome shotgun sequence.
ACCESSION   DAOSZP010000184 DAOSZP010000000
VERSION     DAOSZP010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3560)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3560)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3560
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21017_length_3560_cov_3.170613"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(40..406)
                     /gene="rnpB"
                     /locus_tag="PK348_05045"
     ncRNA           complement(40..406)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PK348_05045"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(410..1000)
                     /locus_tag="PK348_05050"
     CDS             complement(410..1000)
                     /locus_tag="PK348_05050"
                     /inference="COORDINATES: protein motif:HMM:NF015559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HXK65612.1"
                     /translation="MASQHTMRVIGGSFKGRIIPFEKKLKGAEATSQRFKKALFSILG
                     EDLTGKNFLDLYSGTGQIGLEALSRGASLVVFNDSDRNSYQFVKDIVLMWKLSGRSLH
                     YNIDATRCISLCGERGVVFDVVFCDPPFEKIASIPAVYSNILQAISDSSIINEDSVIL
                     VQHYHTNIMPDTLGRLIKKDTRKYGTNSLSIYTVVL"
     gene            complement(1004..1210)
                     /locus_tag="PK348_05055"
     CDS             complement(1004..1210)
                     /locus_tag="PK348_05055"
                     /inference="COORDINATES: protein motif:HMM:TIGR03980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HXK65613.1"
                     /translation="MKITEDMKLKDVITNHVQVAKVFKKYNLNCIGCKGADEDTIRIV
                     ATNYGLDVQQFVKELNEAIKEAKN"
     gene            1644..1832
                     /gene="rpmB"
                     /locus_tag="PK348_05060"
     CDS             1644..1832
                     /gene="rpmB"
                     /locus_tag="PK348_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012531640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HXK65614.1"
                     /translation="MAKCEVCGKAVLFGHNVSHSNKKTQKVWHPNVQKIRVVEGNKVV
                     RKNVCTRCIRSGAVRKAI"
     gene            complement(1836..2423)
                     /locus_tag="PK348_05065"
     CDS             complement(1836..2423)
                     /locus_tag="PK348_05065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65615.1"
                     /translation="MNIVQLIDYFYNAIVDPEKLSQIIAVEDKKHFAISAIVIAVVVF
                     MDIVAQSLLTVQSDFFYYKLTYGFVALFLINIVCIVVYAGLLTITLQIAGYQASGGFI
                     LNALALAQVPRIFIVPVVLIFHSLYFAPVFFYSFGSLALILWSIIIAMRCISQRYSLE
                     AIKAIGYLAAPVIVAGVVGFLIFVAGVMVIIGLLS"
     gene            complement(2420..3349)
                     /gene="sppA"
                     /locus_tag="PK348_05070"
     CDS             complement(2420..3349)
                     /gene="sppA"
                     /locus_tag="PK348_05070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HXK65616.1"
                     /translation="MDKNRRILIAILVILVFAVVLAIIQISITMQRQTTLGTRITLTE
                     GPGVGVVRVYGRIESTGDMLSQANADYVVKRLDELAQDSRIKAIVVRINSPGGTVAAT
                     QEIYNKILSLKKKNIPVVASMGDLAASGGYYIASACNAIVANYGTITGSIGVIAIAPN
                     LRGLFEKLGIRMNVIKSGKYKDILASYRELSPEELQLIQQIIDTSYQKFVHDVAVGRN
                     MAVSDIMPYADGRVFTGSQALEYKLVDELGTFEDAVNKARQLAKLPEDSAVYEDTASP
                     FERILATMSSLVKPKLEQYINTNYSLVEYSYLP"
BASE COUNT         1095 a          774 c          602 g         1089 t
ORIGIN      
        1 ggaaaagata catctctaat attaaaaata tagcgaaaaa taaagagggg tccataagcc
       61 ggattctgtt ttgacagcca tcaatcttgc atgcatgttg ccatgcatgt ccacgcggcc
      121 tacccgcaac ctcaagcggg ccactttagt agcggttgcc tatttggcct tgcaccggat
      181 ggggtttgca ctgccacctg cattactgca gatgcggtga gctcttacct cgccttttca
      241 cccttacccc tgacggggcg gtatgttttc tgctgcactt tccatcgcct tgcggcgccc
      301 gggtgttacc cggcatcctg ccctgcggtg tccggacttt cctcacagcg ctgtacagac
      361 acaatacagt gtttatacag cgtgcgtatg ctgtctgtac ccctcttact tataatacta
      421 cggtatatat acttaatgaa tttgtgccat attttcgcgt atcttttttt attaaccttc
      481 caagggtatc aggcattatg ttagtatgat aatgctgcac aagaattacc gaatcttcat
      541 ttataatgct actatcagat attgcctgca atatgttgct atacactgca gggatactgg
      601 ctattttttc aaatggtgga tcacaaaaca caacatcaaa tacaacaccc cgttcaccac
      661 atagtgatat acatctggtt gcatcaatgt tgtaatgcag tgaccttcca gataatttcc
      721 acataagaac aatatcctta acaaactggt agctgtttct atcgctatca ttaaacacaa
      781 ccagtgaagc acccctgctc aatgcttcca gcccaatctg accggtacca gaatacaagt
      841 ccagaaaatt tttgcctgtt aaatcctcac ccagtattga aaataacgct tttttaaaac
      901 gttgactggt agcttcagct ccttttagct ttttttcaaa cggaataatg cgccccttga
      961 agctgccacc aataacacgc attgtgtgct gtgatgccat acactaattc ttagcctctt
     1021 taatggcttc attgagttcc ttaacaaact gctgtacatc aagcccataa tttgttgcca
     1081 caatccgtat agtatcctca tctgctccct tacatcctat acaattcagg ttatatttct
     1141 taaaaacttt ggccacctgt acatgattgg taattacatc tttcaatttc atgtcttcag
     1201 tgattttcat agtataaagt ctcccaaaaa taatttttca cctcacgtat taatatacat
     1261 acgtaagggt aaagctgcaa tattaaaata tatatcaaaa ctcaccagta aatataattt
     1321 acttaaaaaa ttccaataaa attgttgcaa tttttacaag tcataaaaat tattgtctat
     1381 cattttttta agagtactat ctgtaattat tgtaacatgg tttacaagca aataaccgta
     1441 taacgccatc attaaagatg gcatagatgt atactatcta tttttattca taatcatatt
     1501 ttttgataca gcagcgtgtt aataaaaaat tgacaaatca gcatttttta gctattatat
     1561 aggcaactat cagttataaa tacgtcctat tcatattgct aaatgctatc atataatcat
     1621 taatacagga gcaggagata acaatggcta aatgtgaagt gtgtggaaag gctgtattat
     1681 ttgggcacaa tgtaagccat tccaataaaa agactcagaa agtgtggcat cctaatgtac
     1741 aaaaaattcg tgtggttgaa ggcaataaag tagtacgaaa gaatgtatgt acacggtgca
     1801 tccgttcagg tgctgtacgt aaagcaatat aacaactacg ataaaagccc aattattacc
     1861 ataacaccag caacaaaaat tagaaagcct acaacccctg caacaatgac aggtgcagct
     1921 aagtaaccta ttgccttgat agcttccagt gaataccgct gcgaaataca ccgcatggca
     1981 ataatgattg accacagtat cagtgcaagt gacccaaaac tataaaaaaa caccggtgca
     2041 aaatataacg aatgaaatat aagaacaact ggcacaataa aaatccgtgg tacctgcgca
     2101 agtgccagcg catttagtat aaatccacct gatgcctgat atcctgcaat ctgtaatgtt
     2161 atggtaagca atcctgcgta taccacaata cacacaatat taatgagaaa aagcgccaca
     2221 aaaccatagg tcagcttata gtaaaagaag tccgactgca cggttaaaag ggattgcgca
     2281 acaatatcca taaaaactac aactgctata actattgcac ttatggcaaa atgtttttta
     2341 tcctcaacag caataatttg cgaaagcttt tcaggatcaa ctatggcatt ataaaaataa
     2401 tcaatcaact gtactatatt catggcaggt agctgtactc tactaaagaa taatttgtgt
     2461 ttatatactg ttcaagtttt ggttttacca atgaagacat ggtggcaagt attctttcaa
     2521 aggggctggc agtatcttca tacaccgcac tatcttcagg caattttgca agctgtcgtg
     2581 ccttgtttac agcatcctca aaggtcccca attcatcaac cagcttatat tctaaagcct
     2641 ggcttccggt aaatactctg ccatcagcat atggcataat atcgcttact gccatgtttc
     2701 ggccaaccgc aacatcgtgc acaaacttct ggtagcttgt atcaatgatc tgctgaatca
     2761 gttgcaactc ctccggtgac agttccctgt atgatgcaag tatatcttta tatttgccac
     2821 ttttgatgac attcatacgt attcccaatt tttcaaaaag cccgcgtaaa ttaggagcta
     2881 tggcaataac gccaatggag ccagtaatgg tgccataatt ggcaacaatg gcattgcacg
     2941 ctgaagcaat ataatagcca cctgatgcag ctaaatcgcc cattgatgca acaaccggta
     3001 tatttttttt cttgagcgat agtatcttgt tataaatttc ctgtgttgcg gcaacagtcc
     3061 cgcctggtga gttaatccgc acaactatcg cttttatgcg actgtcctgt gccagttcat
     3121 ctaaacgttt aacaacataa tcagcatttg cctgcgaaag catgtcgccc gtggattcaa
     3181 tgcggccata tacccgcacc actccaacac caggcccctc tgtaagagtt atacgtgttc
     3241 ctaacgtagt ctgccgctgc atggtaatac tgatttgaat gatagccagt acaaccgcaa
     3301 aaaccagtat aaccagtatt gcaatgagta ttcgcctgtt tttatccata gtacgtttcc
     3361 ttttattttc gttatggtac tataatcagt ctattttatg caacattttt tttcacaagg
     3421 gaatgtggtt caagtttttt ttggcgatag ttactgtaat aatatttttt gtctgttact
     3481 attcttttcc catatacatt tattcaaact ttcttttatc ctggtggcaa caaaagaaag
     3541 aattttttta caatatatat
//
LOCUS       DAOSZP010000185         3557 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_21041_length_3557_cov_3.168475, whole genome shotgun sequence.
ACCESSION   DAOSZP010000185 DAOSZP010000000
VERSION     DAOSZP010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3557
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21041_length_3557_cov_3.168475"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1047)
                     /locus_tag="PK348_05075"
     CDS             complement(<1..1047)
                     /locus_tag="PK348_05075"
                     /inference="COORDINATES: protein motif:HMM:NF016827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65617.1"
                     /translation="MAFLKLDVVQTQRLRLTQSLRQSLDLLQIPTLELEEILLQELEE
                     NPVLECDDTLDEGFESLDTVAKKEIEGISPKEEYTAEYESEDTPAFDYDKKNVMEIVS
                     QAETLQEHLLSQARMLQLAPDEMTMLEELITSLDDNGFLPASIEDLQKELGGDSSKIE
                     KLLSIIHTLDPVGCGATTVTDSLRIQAQYFYPDDVLLQQIIKDYLPHIQRYEYDKVAK
                     QLNISSDVVKEKAILLTSLTPYPGAHFFTRKPLYVRPDIEVQLIDDEVVINLLDDWMP
                     KLYINARYIEMLKQKKVDEKTREFIHDKVNAARNLINNISRRNETLLLVAYAIMNRQK
                     EFLQKGPGYLKPLIY"
     gene            complement(1153..2781)
                     /locus_tag="PK348_05080"
     CDS             complement(1153..2781)
                     /locus_tag="PK348_05080"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016544824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HXK65618.1"
                     /translation="MKDKKTKFIFVTGGVCSSLGKGISTASLGLLLEGHGYKLSIIKM
                     DPYINIDPGTMSPFQHGEVYVTEDGAETDLDLGYYERLTNAKLTRKNSVSTGQIYYEV
                     IMRERRGDYLGRTVQVIPHITNEIKKRIYDVANEDDLDFLLVEIGGTVGDIESIPFLE
                     AIRQIRQELGKTRVLNVHLTLVPTITVAGEQKTKPTQHSVKELMELGILPDILLCRSS
                     RPLTDDLKSKIALFCNVSERNVISAVDISHSIYEIPYMLHENKYDEVVLEHFGLPVKQ
                     LHLPEWDAFIKSLMNPKKKVTIAVVGKYISLQDSYRSIYEALTHGAVANNAELEIVRV
                     ESEEIERKYMGKLDELFKGVNGILIPGGFGNRGIEGKIATIEYARVNKIPLFGICLGL
                     QCAVIEFARHVCGMENANSTEFDPTTPYPVISLLEEQELIDKLGGTMRLGAYLCVFKD
                     GTKIKSIYNADSAMERHRHRFEFTLKYKQAFEDNGMVLSGFHPENNLVETIEYRDHPW
                     FICTQFHPEFKSKPVKPHPLFKDFIRASLENIRQ"
     gene            2980..3357
                     /locus_tag="PK348_05085"
     CDS             2980..3357
                     /locus_tag="PK348_05085"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HXK65619.1"
                     /translation="MTEKRQHPRKECNIKVKFDYFEGDPEKVTTSQKPAIKSKGYILN
                     ISTSGAFMVCNCRVSVQMPVKLYCKINKTKLELTGEIVRTGMIQNNPSEVAQRYAAKK
                     VRGDSYVAIRFNELLPEEILSQV"
     gene            3364..>3557
                     /locus_tag="PK348_05090"
     CDS             3364..>3557
                     /locus_tag="PK348_05090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65620.1"
                     /translation="MKTRFIRIDDISQIDTSKISTFDLANRYIDKYGNMYGLRYNRAQ
                     KKVEIVKIMRTPKKHASYYS"
BASE COUNT         1136 a          740 c          609 g         1072 t
ORIGIN      
        1 atagattaaa ggttttaaat agcctggccc tttttgtaaa aactctttct gcctgttcat
       61 tattgcatac gcaacaagaa gaagtgtttc gttccgcctg ctgatattgt ttataagatt
      121 tcttgcggca ttaactttat catgtataaa ttctcttgtt ttttcatcaa cttttttttg
      181 cttcagcatc tcaatatacc gggcattaat atacaatttt ggcatccagt catcaagaag
      241 gttgataacg acctcatcat caatcagctg aacctcaata tcagggcgta catacagtgg
      301 cttacgggta aaaaagtgtg caccaggata tggcgtcaaa ctggtaagga gtatagcttt
      361 ctctttcact acatcgctac ttatattcag ttgttttgca actttatcgt actcataacg
      421 ctgaatatgt ggcagataat cctttatgat ctgttgcaac aggacatcat caggataaaa
      481 gtattgtgcc tgaatacgta atgaatcggt aacagtggta gcaccacacc caacaggatc
      541 cagtgtgtgt attatgctca gaagtttttc tatttttgaa ctatcgccac ctaattcttt
      601 ttgcaaatct tctatagaag caggtaaaaa tccattatca tcaaggctgg taataagctc
      661 ttcaagcatg gtcatttcat caggtgccag ttgcagcatc cttgcctgag acaataaatg
      721 ctcctgcagt gtctcggctt gagaaacaat ttccattaca ttctttttgt cataatcgaa
      781 cgcaggagta tcttcgcttt catattctgc agtatattct tcctttggcg aaatcccttc
      841 tatttccttt ttagcaacag tatcaaggga ttcaaatcct tcatcaagcg tgtcgtcaca
      901 ctccagaaca ggattttctt ccagttcctg caataatatt tcctctaatt caagtgtagg
      961 tatttgtaat aagtctaaag attgtctaag actctgggta aggcgtaaac gctgggtttg
     1021 taccacatca agctttaaga aagccattca ctccctcgca acaatcttcc tcaattcaga
     1081 tattccttta ctacaaccac attcttacat aaatatacaa aaattacaac ccaattgtcc
     1141 atttaaattg cattattgcc ttatattttc aagcgaagcc ctgataaaat ccttaaacaa
     1201 aggatgaggc tttacaggct ttgacttgaa ttcagggtga aattgagtac agatgaacca
     1261 cggatgatcc cggtattcga tggtttcaac cagattattt tcaggatgaa agcccgacag
     1321 caccatgcca ttatcctcaa atgcctgttt atatttaagg gtaaactcaa aacggtgtct
     1381 gtgacgttcc attgccgaat cagcattgta aattgattta atttttgtgc catctttaaa
     1441 tacacataaa tatgccccca gccgcatggt acccccaagc ttatcaataa gttcctgctc
     1501 ttccagaagc gaaataacag gatagggcgt tgtaggatca aattcagtgg aatttgcgtt
     1561 ttccatacca cacacgtggc gggcaaactc aataacagca cactgtaatc ccaggcaaat
     1621 gccaaatagc ggaatcttat taacacgggc atattcaata gttgctatct taccttcaat
     1681 acctctgttc ccaaaacctc cgggtattaa tatgccgtta acccctttaa aaagttcatc
     1741 cagtttgccc atgtatttgc gttcaatctc ttcagattca acccgtacga tttcaagttc
     1801 tgcattattg gcaacagcac catgagtaag cgcttcatag attgagcggt atgaatcctg
     1861 cagcgaaata tatttcccta caacagcaat ggtgaccttt ttctttggat tcatcaggga
     1921 tttaataaat gcatcccatt caggaagatg caactgttta accggaagac caaaatgctc
     1981 aagcaccacc tcatcatatt tattttcatg aagcatatat gggatttcat aaatggaatg
     2041 gctgatatca acagccgaga taacatttcg ttccgagaca ttacagaata aagctatctt
     2101 gcttttcaaa tcatcagtaa gcggccgtga tgaacggcac agaagtatat caggcaatat
     2161 acctaattcc ataagttctt ttactgaatg ctgcgttggc tttgtctttt gctcgccagc
     2221 aactgttatt gttggcacca gtgtcaggtg aacattcaac actcttgttt tacctaactc
     2281 ctgccgtatc tgccgtattg cctctaaaaa tggaattgat tcaatatcac caactgttcc
     2341 accaatctca acaagcaaaa aatcaaggtc atcttcattt gctacatcat aaattctctt
     2401 tttaatttcg ttggtaatat gtggaataac ctgaactgtc ctgcccaaat aatcaccacg
     2461 gcgttcacgc atgatcactt catagtatat ctggcctgtg gacacagaat ttttacgagt
     2521 aagcttagcg ttggtaaggc gctcataata tcccaggtct aaatcagttt cagcaccatc
     2581 ctcagtaaca tatacctcac catgctggaa cgggctcatg gtaccagggt caatattaat
     2641 atacgggtcc atctttatga tggaaagttt atacccatgt ccttcaagta acaatcccaa
     2701 tgaagctgta gatatacctt ttcccaatga agagcatact ccaccggtaa caaaaatgaa
     2761 tttggttttt ttgtctttca tatatcctct aacagatagt atccatgata tcgtaagcca
     2821 taaacagtaa ttacagatac ctgtcaagaa tttgtaaaaa tgtaatcaaa attgatatca
     2881 aaaaaaacaa ttcttgacaa aacactgaag ttactatagt aacagttttg tgttctgtaa
     2941 agaaaaaata aaaaaatttt tataatacag ggaaatgcca tgacagaaaa acgtcaacat
     3001 ccacgaaagg aatgcaatat caaggtcaag tttgattatt ttgaaggtga tccagaaaaa
     3061 gttacaacaa gtcagaaacc agcaataaaa tcaaaaggat atattcttaa tatcagcaca
     3121 tccggcgcat ttatggtatg taactgccgc gtcagtgtac agatgccggt aaaattgtac
     3181 tgtaaaatta ataaaactaa actggaatta acgggtgaaa ttgtacgcac aggaatgatt
     3241 caaaataatc cttccgaagt agcacaacga tatgcagcaa aaaaagtcag gggcgatagt
     3301 tacgttgcta tacgctttaa tgaactgtta cctgaggaaa tactgtcaca ggtataaaca
     3361 ctgatgaaga cacgatttat ccgcatagac gatatatccc agatcgatac atccaaaata
     3421 agtacatttg atttagctaa ccggtatatt gacaaatatg gtaacatgta tggattgaga
     3481 tataatagag cccaaaaaaa agttgaaata gtaaaaatca tgcgcacacc taaaaagcat
     3541 gcatcatatt attcgca
//
LOCUS       DAOSZP010000186         3557 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_21042_length_3557_cov_2.901199, whole genome shotgun sequence.
ACCESSION   DAOSZP010000186 DAOSZP010000000
VERSION     DAOSZP010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3557
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21042_length_3557_cov_2.901199"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..317
                     /locus_tag="PK348_05095"
     CDS             <1..317
                     /locus_tag="PK348_05095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65621.1"
                     /translation="RTGPFWNERYKDSIIEFARDGFHYLLWLLWYLAYNPVRKNLCSD
                     PTQYRYSSIQAYLDEHADVGVPIDHHEYFMQLGDSFAQRVARFLRYDEAYRKRYSIAG
                     WV"
     gene            complement(344..859)
                     /locus_tag="PK348_05100"
     CDS             complement(344..859)
                     /locus_tag="PK348_05100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65622.1"
                     /translation="MLNKFIQILLLWAIVTANVACSYVADYIEGEITNRARFSISAHY
                     EAGTGVIVEWDANPGNDSFAGYEIYMTQEPNNEYSDYFVIGAPYDIGTSSYFQIDGAL
                     SNSSTTQFIHTSLPPSGIYFYRVGVIEWDERDYNNDGEDEKKPASPTPALYKLYTNIA
                     EISGSAMVEIP"
     gene            complement(864..2207)
                     /locus_tag="PK348_05105"
     CDS             complement(864..2207)
                     /locus_tag="PK348_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65623.1"
                     /translation="MRALHHYTIPFIVLLCIHLFAVNTGSFAQYVYEASTLHNAASRS
                     QTVQQIFYQRWDTIFSNYLHFDAYIHLNWVYDFYVNSYNRGTGTTQAVPMRLVRTFSD
                     IALLFPVYTPVTETPDEAQPVDITDVSTFRKPWVDNSILLGFTACGYHYGLTRTVDIN
                     RGSAGTESASDYAFSQFFDDIVAITAVFVPYVTLHAGIVFNQQIEPNEDGTLDYNFDP
                     STITKRYFASVSLYNILTWRSTAAKDRLESYDISLNITQFLSITTTIPSYLPQITIGY
                     KKYQKYNDEPYDPVWVSTASGKSNTMSDSLKQHATLYTYYTTLSKNFGNFFLATSIEL
                     QHVDRTIVDKRTEQKVSLPVMRLWWAKAGYDLMDRPGWDFIVWLGVNKFYDPAMSYHS
                     KHSDNDTGGWFAMIDGTSPYITLSIKASYNDYSELDKLIEAIDKYIIEGSASFRI"
     gene            2578..>3557
                     /locus_tag="PK348_05110"
     CDS             2578..>3557
                     /locus_tag="PK348_05110"
                     /inference="COORDINATES: protein motif:HMM:NF012239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HXK65624.1"
                     /translation="MMLKEYSTENVRTVAIVGHGSTGKSTLFDAMLFICGQIDKIGKP
                     DDGSLTSDYDEEEKSRKISIRSALGFVEIDDVKINIIDTPGMSDFVGEARAAIQVAEA
                     AIVVVDAVDGVQIETEKVWRYLESKKIPRIIFINKMDKERANFNAIIDNLKSHFNAKF
                     AAVCIPVGEADKHSGIVDLIEMKAMMPKPDGKIQVSDIPADLKKAAEDARNSLMELSA
                     EGDDALIEKYLEGEPLTEDEMKKGLKALVARAEVFPVICGSSLKAIGIKNLLNVIKNY
                     VPAWELNKQVEGLNPNNKEEKVVITSKPDAPFAAVVWKTYIDQYAGRFNY"
BASE COUNT         1129 a          605 c          750 g         1073 t
ORIGIN      
        1 gaagaacggg tccgttctgg aatgagcggt ataaggatag catcatagag tttgccaggg
       61 atgggtttca ctatttgctg tggttgttat ggtatttagc gtataatccg gtgaggaaga
      121 atttatgctc tgaccccacc caatatcgct acagcagcat acaggcatac ttagatgagc
      181 atgccgatgt aggagtacct atagaccatc atgagtactt tatgcaactg ggggatagtt
      241 ttgcccaaag ggtagcacgc tttttgcgct atgatgaggc atatcgcaaa cgatattcta
      301 ttgcaggctg ggtgtaaaga acaaaagggt cagagccatg taattatgga atttccacca
      361 tcgcactgcc actgatttca gctatatttg tatataattt atataatgca ggggtaggtg
      421 aagctggttt tttttcatct tcgccatcat tattatagtc gcgttcatcc cattcaatga
      481 cgccaacccg gtagaaataa atgccagatg gtggtaaaga agtatgaata aattgtgtag
      541 ttgaagaatt tgatagcgcc ccatcaattt gaaaatatga tgaagttcca atgtcataag
      601 gtgcaccaat aacaaaataa tcggaatatt cattgtttgg ttcctgggtc atgtaaattt
      661 cataaccagc aaatgagtca ttgccaggat tagcatccca ctcaacaatg actccggtac
      721 cagcttcata gtgggcactg atggaaaaac gggcgcggtt ggtaatctct ccttcaatat
      781 aatctgctac gtaggaacag gctacattgg cagtaactat cgcccacaat aataatattt
      841 gtataaattt gtttagcata gtattatatc ctgaacgatg cacttccttc aatgatatac
      901 ttgtcaatag cttctatcag cttatcaagc tcactgtaat cattatatga agctttgatg
      961 gacaacgtaa tatatggtga tgtgccatca atcatagcaa accatccccc tgtatcatta
     1021 tcgctgtgct tactgtgata gctcatagca gggtcataaa acttgtttac tccaagccat
     1081 actatgaaat cccaaccagg tctatccata agatcatatc cggctttagc ccaccatagc
     1141 cgcattacag gcagtgatac tttttgttct gttcgtttat caacaattgt tctatctaca
     1201 tgttgaagct caatgcttgt ggcaaggaaa aaattaccaa agtttttgga taacgttgtg
     1261 taataggtat acagtgttgc atgttgcttt aaactatcgc tcatggtatt tgatttgccg
     1321 cttgcagtgc ttacccatac cgggtcatat ggctcgtcat tgtacttttg atatttttta
     1381 tagccaattg ttatctgcgg caggtatgaa ggtatagttg ttgtaatgga taaaaattgt
     1441 gttatgttca ggctgatatc gtaggactca agacggtctt ttgcagccgt tgagcgccag
     1501 gttagtatgt tataaagact tactgatgcg aagtagcgtt tggtaattgt tgatgggtca
     1561 aaattataat ccagcgtgcc atcttcattg ggctctatct gctggttaaa gacaattcct
     1621 gcatgaagcg taacataggg aacaaacact gctgtgattg caacaatgtc gtcaaaaaat
     1681 tgtgaaaagg cataatcaga tgctgactct gttccggcac ttccacggtt aatatcaaca
     1741 gtacgggtta agccatagtg atatccacag gctgtaaatc caagcaaaat tgaattgtcc
     1801 acccatggct tgcggaatgt tgatacatcg gttatatcaa ctggctgagc ttcatctggt
     1861 gtttcggtaa ctggtgtata aacgggaaat aacaatgcta tatccgagaa tgtcctgacc
     1921 agacgcatgg gaacagcctg agtggtaccg gtaccacgat tgtatgagtt aacataaaaa
     1981 tcatataccc agttgaggtg tatatatgca tcaaagtgca gatagttact gaatatggta
     2041 tcccagcgct ggtaaaaaat ctgctgtaca gtctggcttc gcgaggcagc attatgcagt
     2101 gtggatgctt catagacata ctgtgcaaaa ctaccagtat tcactgcaaa caggtgaata
     2161 caaagtaaaa cgataaaagg gatggtataa tggtgtaatg ctctcataat gttatggata
     2221 ttttttatgt aactcatgaa tattttacaa tatgaaattg taataagaat atgttatcaa
     2281 atataaatac tgtatccaga aattagataa tgttatctat acctgatgct ttttggtgca
     2341 aactacatac tattaccgga ataatcaaga ataaaatatg tataaccata ttcatatact
     2401 atggtaaata tcaatgtatt gtataaaaaa gaataactgt atcactgctt tcaattttat
     2461 aaaataaaat actttacaaa aaacatatac cattttactt ttctgcaaaa gcttttgtct
     2521 tttcgtccta aaagagaata tctcatttta atttagtgaa taagtacgct ggagggtatg
     2581 atgctaaaag aatattctac tgaaaatgtc agaactgttg ccattgtggg tcatggaagt
     2641 accggaaaat ctacattatt tgacgcaatg ttgtttattt gtggtcaaat tgacaaaata
     2701 ggtaaaccgg atgatgggtc gctgacatcg gattatgatg aggaagaaaa aagcaggaaa
     2761 atatcaatac gcagtgcact gggttttgta gagatagatg atgttaaaat aaatataatt
     2821 gatacacctg gtatgtcaga ttttgttggt gaagcacgag ctgcaattca ggtggctgaa
     2881 gcagctatcg tagtggttga tgcagttgat ggtgtacaga tagaaactga aaaagtatgg
     2941 cgttatttag aatcaaaaaa gatcccccga atcattttta taaataagat ggataaagag
     3001 cgggcaaatt ttaatgcaat tattgataat ctgaaatccc attttaatgc caaatttgca
     3061 gctgtttgca ttcctgttgg tgaagctgat aaacacagtg gcattgtgga ccttattgaa
     3121 atgaaagcta tgatgccaaa acctgatggc aaaattcagg taagcgatat accggcagat
     3181 ttgaaaaaag ctgcagaaga tgcacgcaat agtttgatgg aactatcggc tgaaggcgat
     3241 gatgcactca tagaaaagta tttagaaggt gaaccgttaa ctgaagatga aatgaaaaaa
     3301 ggcttgaaag ctcttgttgc cagagcagag gttttcccgg ttatatgcgg ttcttcactg
     3361 aaagccattg gtataaagaa tttacttaac gttattaaaa attatgttcc tgcgtgggag
     3421 ttaaacaaac aggttgaagg cctgaatccc aataataaag aagaaaaagt agttatcaca
     3481 tcaaaacctg atgcaccatt tgctgctgtt gtgtggaaaa catatattga ccagtatgcc
     3541 ggcaggttta actattt
//
LOCUS       DAOSZP010000187         3523 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_21259_length_3523_cov_3.369377, whole genome shotgun sequence.
ACCESSION   DAOSZP010000187 DAOSZP010000000
VERSION     DAOSZP010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3523
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21259_length_3523_cov_3.369377"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..254
                     /locus_tag="PK348_05115"
     CDS             <1..254
                     /locus_tag="PK348_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018749461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HXK65625.1"
                     /translation="NTERVRTWDSCSYEGYSRMAGGHNPRKPIEDRRNKRFFCKLSYS
                     QSKKYLRPGCVGCGRCAWVCPGDIGLPNVVTYIRREIHE"
     gene            264..1097
                     /locus_tag="PK348_05120"
     CDS             264..1097
                     /locus_tag="PK348_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020505777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HXK65626.1"
                     /translation="MSEQKIVTVPEIATIEEIKDEIVDVKTFYLRFDNKEIDGNFKFK
                     SGQFIMCTVFGAGEFAVSLPPSPENDRFHITVRRIGKVTNALHDLQVGDKVGIRGPFG
                     NGFPFEEIKGKNVIYVAGGIGLIPLRSSIVHVLQHRNEFGRILLLYGSRSPQDLMYQY
                     MLDEWQKIEGFETFITVDNGTPEWKGNVGIITTLFDKVEIPVENTVAFVCGPPVMFNA
                     VIKELMQRGIKDDMIISTLERHMKCGVGKCQHCAIGRTLVCTDGPVYTYRQIKTLGEQ
                     I"
     gene            1094..1600
                     /locus_tag="PK348_05125"
     CDS             1094..1600
                     /locus_tag="PK348_05125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HXK65627.1"
                     /translation="MSWLQTMYTMFKDTTCIVGVGNYYRSDDAVGLYIVDALAAKVVQ
                     PHVVIINVEDVIESYVFAIAESNCKNVLIIDAIEADAEEGTVVFGKLSDFVETGLDVS
                     THKLALRMSCKILEASGKDVYMLGIVIKHNDFGKGLSPIIQNNIDSIVSMVQSSIMSN
                     KKECVYEH"
     gene            1590..3449
                     /locus_tag="PK348_05130"
     CDS             1590..3449
                     /locus_tag="PK348_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018749464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HXK65628.1"
                     /translation="MNIDLYGMLLQAFAILLGTSIIVPLIASKRKLAGWINTIAVAIA
                     AGLLMYVAYMAIFVQQAAETQAIYFGSVPVYFLIDAFSGFFIAIISFMAVMSALYSID
                     YMEHYKDYSLVPYYINFPLFILGMIGIVTVDDLSIGFTVAWQLMTIASYFLIRFEYKE
                     KQNVRNANKYLILMELAWVFIVVGAFLINGSAMGDSIHTLTQKLGTSGGMIPAVYALL
                     LLGFSFKAGVFPFGQLWLPDAHSIAPSPVSALLSGVMIKTGIYGIVRTFFWMIPHEGH
                     TVNPLVWGSIIATFGVVTLFIGTSQSLKQSDAKRLLAYSSIGQIGYIVLAIGAGLYMY
                     HSSNMFVQMLSFLVIIGILYHVLNHAIFKGLLFLTAGSILYETGTKDLNKLGGLLKFM
                     PVSAVIAGIASLSIAGVPPFSGFASKWTIISATLLGGSQVFFLALFGIIALFTSALTL
                     ACYVKFFGMSFASTGVEWNVEHKPQEVSWKMLVPKIILGLLCIVQGIVPFIYYETIMN
                     IFRSSTGSIVQNSFNELSAHNAVFTNALGVTINVPGMQYANAIAVPLVVIVIVIIGFA
                     VAYMLKKSAGSQEKVMPTWLCGYQDLNNNNRYKDRHMFAALKSMLWWTGGNVK"
BASE COUNT         1029 a          580 c          826 g         1088 t
ORIGIN      
        1 gaaatactga gcgcgtgcga acatgggatt catgttcata cgaaggatat tccagaatgg
       61 ctgggggtca taatccacgg aaaccaatag aagacagaag aaacaaacga tttttctgta
      121 aactgtcata ctcacaatca aagaagtatc tgaggccggg atgtgttgga tgcgggcgtt
      181 gcgcatgggt atgtcctggt gatatagggt tacccaatgt tgttacatat atccgaagag
      241 aaattcatga ataggtgata gctatgagcg aacagaaaat agtaacagtc ccagagattg
      301 ccacaattga agaaattaaa gatgagattg ttgatgtaaa aaccttttat ctgaggtttg
      361 acaataaaga aattgatggc aatttcaagt ttaagtcagg ccagtttatt atgtgtacag
      421 tatttggtgc aggtgaattt gcagtatcat tgccacccag tcctgaaaat gaccgcttcc
      481 atataaccgt acgacgaata gggaaggtaa ccaatgcatt gcatgatctt caagttggtg
      541 ataaagtggg aatacgtgga ccatttggca atgggtttcc gtttgaagaa ataaaaggta
      601 aaaatgttat ctatgtggct ggtggtattg ggcttattcc tttgcggtca tcaattgtcc
      661 atgtattgca gcaccgtaat gagtttggcc gaatcttgct tttatatggg tcacgttcac
      721 cacaggatct aatgtatcag tatatgctgg atgagtggca aaaaattgaa gggtttgaaa
      781 catttattac agttgataat ggcactcccg aatggaaagg taacgtgggt attataacaa
      841 ccctatttga taaagtagag atacctgtgg aaaatactgt agcatttgtc tgtggccctc
      901 cggtaatgtt taatgctgtt ataaaagaac ttatgcagcg tggaattaag gatgacatga
      961 taatctcaac gttagagagg catatgaagt gcggcgtggg aaagtgtcag cactgtgcca
     1021 tcgggagaac cctggtgtgc actgatggcc cagtatatac ctacaggcag attaaaacgt
     1081 taggtgagca aatatgagct ggctacaaac aatgtatact atgttcaaag atacaacctg
     1141 cattgttggg gtaggaaact attatagaag tgacgatgca gttggtctgt acattgttga
     1201 tgctctggca gctaaagttg tgcagcctca tgtagtcata attaatgtag aagatgttat
     1261 cgaaagctac gtgtttgcaa ttgctgaatc aaattgtaaa aatgtgttga ttatcgatgc
     1321 tatagaagcg gacgcagaag aaggcacggt tgtttttggc aaactcagtg attttgttga
     1381 aacgggatta gatgtatcaa cgcataaatt ggcattacga atgtcatgca aaatactgga
     1441 agcatcgggc aaggatgtat acatgctggg gatagttatc aagcataatg attttggaaa
     1501 gggactatcg cccataatac agaataacat tgattcaatt gttagtatgg ttcaatcctc
     1561 aattatgagc aacaaaaagg agtgtgtgta tgaacattga tctttatgga atgttattac
     1621 aggcttttgc tatattgctt ggtacaagta ttattgtacc attgatagct tcaaagagaa
     1681 aattagcagg ctggataaac accattgcag tggcaattgc agcaggatta ttgatgtatg
     1741 ttgcatatat ggcaatcttt gtacaacaag ctgcagaaac acaggcaata tattttggta
     1801 gtgtgccagt ctattttctt atagatgcat tttcaggatt ctttattgcc atcatttcgt
     1861 ttatggcagt gatgagcgca ctgtattcta ttgactatat ggaacattat aaggactact
     1921 cgcttgtgcc atactacata aatttcccgc tattcatact ggggatgatt gggatagtaa
     1981 ccgttgatga tttgagtatt ggattcacgg tagcatggca gcttatgaca atagcatcgt
     2041 attttctgat tcgatttgaa tataaagaaa aacagaatgt gcgcaatgca aataaatatt
     2101 taatccttat ggaattggca tgggttttta ttgttgttgg tgcattcctc atcaatggtt
     2161 cggctatggg tgattcaatc catacattaa cacagaagct tggtacatca ggagggatga
     2221 tcccggcagt gtatgccctg cttttgcttg gtttcagctt taaagctggt gtattcccgt
     2281 ttggtcagtt gtggttgcct gatgcacact caattgcacc gtctcctgtt agtgcactct
     2341 tgagtggcgt tatgattaaa accggcattt atgggatagt cagaacattt ttctggatga
     2401 ttccgcatga aggtcatact gttaatcctc ttgtatgggg aagtataatt gcgacgtttg
     2461 gtgtagtgac gttgtttatt ggcacctccc agtctttgaa gcagagtgat gcaaaacgcc
     2521 tgcttgctta cagctccatt ggccagattg gttacattgt tctggctatt ggagctggtc
     2581 tgtatatgta tcacagcagc aatatgtttg tacagatgct gtcatttctg gttataattg
     2641 ggatactgta tcatgtactc aaccatgcaa tctttaaagg acttctcttc ctcaccgcag
     2701 gcagcatatt gtatgaaact ggtacaaagg atctcaataa attaggcggt ttgcttaaat
     2761 tcatgcctgt cagtgcggtg attgcaggaa ttgcttcact gtctatagca ggtgttcctc
     2821 ctttcagtgg atttgcaagc aagtggacaa taatctctgc tacactactt ggtggcagcc
     2881 aggttttctt ccttgccctc tttggcatca ttgctctgtt taccagcgca ttgacactgg
     2941 catgttatgt aaaattcttt ggtatgtcat ttgcttcaac aggtgtggaa tggaatgttg
     3001 aacacaaacc acaggaagta tcctggaaga tgcttgtgcc aaagatcatt cttggattgt
     3061 tgtgtatagt tcagggtata gtccctttca tatactatga aacgataatg aatattttca
     3121 ggtcctcaac tgggtccata gtacagaatt catttaacga actatcggca cacaatgctg
     3181 tgtttacaaa tgcattaggt gtgactataa atgttcctgg tatgcagtac gctaatgcca
     3241 tagctgttcc gcttgttgtg atagttattg tgatcattgg atttgccgtt gcatatatgc
     3301 tgaaaaagag tgctggttct caggaaaaag ttatgccaac atggttgtgt gggtatcagg
     3361 accttaataa taataacaga tataaggaca gacatatgtt tgcagcgtta aagtccatgc
     3421 tgtggtggac aggtggtaat gtgaaataat acaacattta ggaataagag gtaagatatg
     3481 ccatcgatag accaagtaaa aaaagattta aaagaaaagt tca
//
LOCUS       DAOSZP010000188         3517 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_21293_length_3517_cov_2.464471, whole genome shotgun sequence.
ACCESSION   DAOSZP010000188 DAOSZP010000000
VERSION     DAOSZP010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3517
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21293_length_3517_cov_2.464471"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..455
                     /locus_tag="PK348_05135"
     CDS             <1..455
                     /locus_tag="PK348_05135"
                     /inference="COORDINATES: protein motif:HMM:NF012348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HXK65629.1"
                     /translation="WVNSYKRLNYGFEAPIDLSWSINNPSSLIRITTSRTDNPNSVRV
                     ELRNPDPACNPYLLFSAILASGLLGIEHNEPLPKPHNLNETKSGFTTKSLPSTLSDAL
                     KLFEKSSLMREALGDKLVDLFVENKHYELDQYNRYITDYEINTYLPIL"
     gene            462..1490
                     /locus_tag="PK348_05140"
     CDS             462..1490
                     /locus_tag="PK348_05140"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HXK65630.1"
                     /translation="MKPTVALIGCGRIGFLLEDDPLRYKPCTHYGGAKAAKLSITHAC
                     DINEKRLNAFSKIAGIPKSHCYTDYQKLLEEVKPSCVIIASWTHTHVPIGIAAAQLGA
                     KVIVCEKPIASNLTQAKAFINACNTHNTTLIINHERRYDYRYNYVKKLIGKNEIGHIS
                     SVHGFVFTPSKTHQPGSGGGPLLHDGTHLIDIVQYLFGNIAQAQGFTTRYSQDSLYED
                     YAIAHCMTTSGLHVILEAGGHRDYFMFELQIFGTKGRIVIGNGYLYLYKPKTSRYYQG
                     FKDLVALEITPKGNPDYFVKLYKEAKKCLHKPATVTSSGYDGYKALEVIHAIYTSANH
                     NGRIITLG"
     gene            complement(1508..1918)
                     /locus_tag="PK348_05145"
     CDS             complement(1508..1918)
                     /locus_tag="PK348_05145"
                     /inference="COORDINATES: protein motif:HMM:NF017020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HXK65631.1"
                     /translation="MKNEEIFTLIKYRLEEAHNALNDAKFLMENNRSFQSIINRAYYA
                     MFYSVLALLQFIGKIPSKHTGVISLFDTEFVLKDVFPKDLSKMLHKAFELRNTVDYKT
                     CKPISKEKTEETLKNAEYFVKVIYEYLNGQMNKE"
     gene            complement(1921..2241)
                     /locus_tag="PK348_05150"
     CDS             complement(1921..2241)
                     /locus_tag="PK348_05150"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HXK65632.1"
                     /translation="MTDNDIEIIKKFKALLLKKFTNFEVILFGSRARNDSTQYSDMDI
                     VVILDSIKDESDIDYVSDCAWEAGFEYGIVVVPIVFSKDEWDNSPQRYSLLVQAVKSE
                     GIVV"
     gene            complement(2319..2957)
                     /locus_tag="PK348_05155"
     CDS             complement(2319..2957)
                     /locus_tag="PK348_05155"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HXK65633.1"
                     /translation="MFTGIIEEIGVVQSIRQSANGKLISIHARTVIEGTKIGDSIAID
                     GACQTVTQIGKDYFTVFASDITLGITTLGSFKPGTKVNLERAMQPQGRLGGHLVQGHV
                     DATGKIVSLHRLPEGVEISLTVPENISKFIVPKGSIAIDGISLTVVDKKDTIELYCIP
                     ETIANTTLDNKKVGDLVNVEVDIIAKYVYYMVQKIQGGNSSLEKKLKEEGFW"
     gene            complement(2958..>3517)
                     /locus_tag="PK348_05160"
     CDS             complement(2958..>3517)
                     /locus_tag="PK348_05160"
                     /inference="COORDINATES: protein motif:HMM:NF013989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HXK65634.1"
                     /translation="TKQYTAVGYSNSFINGLLELWKPDVTHDVKKIVIGLPKTLDNIK
                     LAGDDNAYFFTTHKEKDALLKRDDYAIIAKLIDSGRIQFLQSDSSVDQVHELLDILHT
                     MGSMIAVLEGGATIAGSFFDSGEIDQCMYFYSPILVGKGKSVIEGKGRQFIKESMTLH
                     DVTSIFLGHDMLISGYKESYQFEAV"
BASE COUNT         1106 a          752 c          556 g         1103 t
ORIGIN      
        1 aatgggtaaa ttcatacaag cggttgaatt atggatttga agcacccatt gatctgtcat
       61 ggagcattaa taatccatca tcactcatcc gtattactac atccagaact gataatccca
      121 attcggtcag agttgaactc agaaaccctg accctgcatg taacccttac ctgctatttt
      181 cggcaattct tgcaagtggg cttttgggca ttgagcataa cgaaccactg ccaaaacctc
      241 ataacctcaa tgaaacaaaa tcaggtttta ccacaaagtc attaccatct accctttcgg
      301 atgcactgaa actatttgaa aaaagcagtc tcatgaggga agccctgggc gataagctgg
      361 tggatctatt tgtagaaaac aaacactatg agcttgatca gtacaatcgc tacattaccg
      421 actatgaaat taatacctat ctccccattt tatagaacac catgaagccc actgttgctc
      481 tcattggctg tggccgaatt ggctttttac ttgaagatga ccctttgcgc tacaaaccat
      541 gtacacacta tggtggcgca aaggctgcca agctatccat aacccatgcc tgtgatatca
      601 atgaaaaacg tttgaacgct ttttcaaaga ttgcaggaat cccaaaatca cattgctata
      661 ctgattatca aaagctactt gaagaggtaa agccttcatg cgttattatt gcatcctgga
      721 ctcatactca tgtgccaatt ggcattgcag cagcacagct tggagcaaaa gttattgtat
      781 gcgaaaaacc aattgccagt aatcttacac aggcaaaagc cttcattaac gcttgcaaca
      841 ctcacaatac aacactcatt atcaaccatg aacggcgcta tgactaccgt tacaattatg
      901 taaaaaagct tattggcaaa aatgaaattg gccacatatc atcagttcat ggttttgttt
      961 ttaccccgtc aaaaacgcat cagccaggct caggcggtgg acctctttta cacgatggaa
     1021 cacatttaat tgatattgtg cagtatcttt ttggaaacat agcacaggca cagggattca
     1081 ctacacgcta ttcacaggat tcactatatg aagattatgc gatagctcat tgcatgacca
     1141 cctcaggcct ccatgtaata ctggaagctg gcggtcaccg tgattacttc atgtttgaat
     1201 tgcagatatt tggcactaag ggcaggatag ttatcggtaa cggctatctt tatctatata
     1261 aaccaaaaac atcacggtat taccagggat tcaaggacct ggttgcattg gagattacac
     1321 caaaaggcaa ccccgattac tttgtgaagc tgtataagga agcaaagaag tgtttacaca
     1381 aaccagcaac cgttacttca agcgggtatg atgggtataa agctctggaa gtgattcatg
     1441 ctatttatac atcagcaaac cataatggaa ggattattac attagggtag aaaatataat
     1501 tatattatta ttctttattc atttgtccgt tgagatattc atatattact ttaacaaaat
     1561 attctgcatt tttcaatgtt tcttctgttt tttctttaga aataggctta catgttttat
     1621 aatctacggt atttcgtaat tcaaaagctt tgtgtagcat tttagaaaga tccttaggga
     1681 atacatcttt taaaacaaat tcagtgtcaa ataaacttat tacacctgta tgctttgaag
     1741 gaattttgcc aataaactgt aataatgcca gaacagaata aaacatagca taatatgctc
     1801 tattaataat gctttggaaa ctacgattgt tttccattaa aaattttgca tcgttcagag
     1861 cattgtgtgc ttcctctaac ctatatttta tcaacgtaaa tatctcttcg tttttcattt
     1921 ctaaactaca ataccttcag atttaacagc ctgaactaat aaagaatacc tttgtggact
     1981 attgtcccat tcatcttttg aaaatacgat tggaacaaca acaataccat attcaaatcc
     2041 agcttcccaa gcacaatcgc taacataatc tatatcactt tcatctttta tagaatccaa
     2101 tataacaaca atatccatat cagaatattg tgttgaatca ttccttgctc ttgaaccaaa
     2161 taaaataact tcaaagttag taaatttttt tagtaataaa gctttaaatt ttttaataat
     2221 ttctatatca ttatcagtca taaaaaacta tcctttaata tcatttttaa ttatcaaaaa
     2281 taatttaccc attgaatcaa gcccctggaa tcctatctct accagaagcc ttcttcttta
     2341 agtttctttt ccagtgaact attgccaccc tgtattttct gaaccatata atacacatat
     2401 ttggcaataa tatcaacttc tacgttaacc aaatcaccca cttttttgtt gtcaagcgtg
     2461 gtattggcaa tagtttccgg aatgcaataa agctctatag tgtctttttt atccacaacc
     2521 gtcagtgata tgccatcaat ggcaattgaa cccttgggta caataaactt actgatgttt
     2581 tctggcaccg ttaaggaaat ttcaacgccc tcaggcaatc tatgcaaaga aacaatttta
     2641 cctgtagcat caacgtgacc ctgaactaaa tgtccgccca gtcgcccctg cggttgcatg
     2701 gcacgttcta aatttacctt ggttcctggt ttaaatgaac caagtgtggt tatgccaagc
     2761 gttatatccg aagcaaacac ggtaaagtaa tccttgccaa tttgtgttac cgtctggcac
     2821 gctccgtcaa tagcaataga atcaccaatt ttagtgcctt caataactgt gcgcgcatgt
     2881 atggaaatta atttcccatt tgccgattgt ctgatagatt gtaccacccc aatttcttct
     2941 ataatgccgg taaacattta caccgcctca aactgatagc tttctttata ccctgatata
     3001 agcatatcat gtcctaaaaa tatactggtt acatcatgaa gagtcattga ttctttaata
     3061 aactgtctgc ctttgccttc aatgacactt ttccctttcc caactaaaat aggactataa
     3121 aaatacatgc actggtcaat ctcacctgaa tcaaagaatg atcctgcaat ggttgcccct
     3181 ccttcaagca ctgcaatcat tgaacccata gtatgcaata tgtcaagcaa ctcatggacc
     3241 tgatccacac tgctatctga ttgtaaaaac tgtattctgc cactatcaat aagctttgca
     3301 ataatggcat aatcgtcccg cttcaaaagc gcatcttttt ctttatgtgt tgtaaaaaaa
     3361 taagcattgt catcgccggc aagcttaata ttatccaatg tctttgggag tcctatcact
     3421 atttttttta catcatgagt tacatcaggt ttccataatt caagcagccc gttgatgaag
     3481 ctattggaat atcccaccgc agtatactgt ttggttt
//
LOCUS       DAOSZP010000189         3510 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_21335_length_3510_cov_3.178003, whole genome shotgun sequence.
ACCESSION   DAOSZP010000189 DAOSZP010000000
VERSION     DAOSZP010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3510
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21335_length_3510_cov_3.178003"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="PK348_05165"
     CDS             complement(<1..290)
                     /locus_tag="PK348_05165"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HXK65635.1"
                     /translation="MDVKAVIDTRRKGEPEMGLFIVAFILIGIGIAMTYSASALFAER
                     TFGDSLYFLKRQLLWSCIGFIIMMFVQQIDYRNYQQYTKIMLVFTLVTLVLVL"
     gene            complement(281..1660)
                     /gene="murD"
                     /locus_tag="PK348_05170"
     CDS             complement(281..1660)
                     /gene="murD"
                     /locus_tag="PK348_05170"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HXK65636.1"
                     /translation="MEQKKHILVVGLGYRTGLASANFLVGKGFDVIVCDRKSHEELLP
                     VIQKLDQSVKVIAGNQDPSILDTGFNEIILSPGVPQSIPLIQEAKGKGIPVISEIELA
                     YRYCTSRIVGITGTDGKSTTTMLTAHCLKASGFDAHPCGNIGIPYVSIVDTLKKDSVA
                     VIELSSFQLETIDTFKPDVAILTNLTPDHLDRYNSLEEYFAAKMNIAKNQDSNDYFIV
                     NSDDEFIATHLPSLKATKFTFSLQKDADCYFKEGGIYCTLNGRQQLIAQASRLKILGL
                     HNVQNAMAAILATLSLAKKIGRDVNLKTLEDALYSFEGLPHRVQMIGSFEGRAFINDS
                     KATTVGAVIMALKSIPGDVILIIGGRAKGDDYSRLKNYLKGKVKALVLIGETKDEFAQ
                     LFNDIPHVKAHSMEDAVVKAMKVSGEGDTILLSPACASFDMYKNYEERGNDFIEIFKR
                     LKQGQLRWM"
     gene            complement(1731..2816)
                     /gene="mraY"
                     /locus_tag="PK348_05175"
     CDS             complement(1731..2816)
                     /gene="mraY"
                     /locus_tag="PK348_05175"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020588786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HXK65637.1"
                     /translation="MFYHFILPLQEYLSFLRLFQYITFRSAYAALTSLLIVLIFGGLV
                     IRWLTHLRFKEEIRTLGPQSHQKKAGTPTMGGVLILGAVLISALLWGNFNNLYFILLI
                     VITVLLGAIGFIDDYIKSILKKKDGIPARLKFLLQCVSGLLISLCIYFFPSNVKEATT
                     LYVPFINRAILDLSWVWILFAMLVIVGSSNAVNLTDGLDGLAIGTVTIVVSVLAVMAY
                     LTGHQPIAQYLRIPYIPQAAELTVFLSALGGAGLGFLWFNSNPASVFMGDTGSLALGG
                     IIGTVAIMIKKEFFLVIVGGIFVIEALSVIVQVASYKLRKKRVFKMAPIHHHFELSGW
                     PEQKVVVRMWIIGIILAILGLSSLKIL"
     gene            complement(2824..>3510)
                     /locus_tag="PK348_05180"
     CDS             complement(2824..>3510)
                     /locus_tag="PK348_05180"
                     /inference="COORDINATES: protein motif:HMM:NF014874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HXK65638.1"
                     /translation="TKHAEIVKKKAREKNINVITFGLTGDIAPESYTLFQDKTVVEYD
                     GVEITVPAYGFHNVYAAIATIAVARIMGKSVIKVKEALSNFKPVAMRSDIKKGRCTII
                     DDCYNANPLSMEYAFTSASMVFPDKRKIAVLSDMKELGTYSRDLHFTVGTRLKASGFS
                     HVLVWGNDARYIAEGAIAGGLDNSLVNVFDSKEELIAFLQQFVKHDDVVLVKGSRSMK
                     MEEVVNALEQ"
BASE COUNT         1094 a          811 c          576 g         1029 t
ORIGIN      
        1 agcaccagta ccagcgttac aagagtaaac acaagcatta tttttgtgta ttgctgatag
       61 ttccggtaat caatctgctg aacaaacatc attattataa aaccaataca gctccataac
      121 aattgccgtt ttaagaaata cagggaatca ccaaatgttc tttctgcaaa aagtgcactt
      181 gcactgtagg tcatggcaat accaatgcca atgagtatga aagccactat aaatagcccc
      241 atctcaggtt cgccttttct gcgtgtatca atgactgcct ttacatccac cgcaattgcc
      301 cctgtttaag tcttttaaag atttcaataa aatcattccc ccgttcttca taatttttat
      361 acatatcaaa cgaagcacaa gcaggtgata gaagtatagt atcgccttct ccacttacct
      421 tcattgcctt aacaacagca tcttccatgg aatgtgcttt tacatgggga atatcgttaa
      481 aaagttgcgc aaactcatcc ttcgtttcac ctattagaac cagtgcctta actttgcctt
      541 ttaaataatt tttcagtcgt gaataatcat caccttttgc cctgccacct ataatgagta
      601 tcacatcacc gggaatactt tttaatgcca tgataacagc accaaccgtt gttgcctttg
      661 aatcatttat aaacgctctt ccttcaaagc tgcctatcat ctgcacccgg tgaggcaagc
      721 cttcaaaaga gtacaacgca tcttcaagtg tcttaagatt aacgtcacgg cctatttttt
      781 ttgcaaggga tagtgtagcc aatattgccg ccattgcatt ctgcacattg tgtagcccca
      841 gtattttaag cctgctagct tgtgctatta attgttgcct gccattcagt gtacaatata
      901 ttccaccttc tttaaaataa cagtcagcat ctttttgcag tgaaaaagta aactttgttg
      961 ctttcaatga aggcagatgt gttgctataa attcatcatc actgtttaca ataaaataat
     1021 cattgctatc ctgatttttt gcaatattca tctttgcagc aaaatactct tcaagcgaat
     1081 tataccggtc taaatggtca ggcgtaaggt tggtaaggat tgcaacatct ggtttaaatg
     1141 tatcaatagt ttccagttgg aatgaggaaa gttcaatcac tgcaactgaa tcttttttca
     1201 atgtatcaac tatcgacaca tagggaatgc caatattgcc gcatggatgt gcatcgaagc
     1261 cagaagcttt caggcaatgt gcagtcagca tggttgtcgt tgacttgcca tctgtccccg
     1321 taatacccac aatacgcgat gtgcaatacc ggtaagcaag ctcaatttct gatatcaccg
     1381 gtataccttt accctttgct tcctgtatta atggtatact ctgtgggaca cctggactta
     1441 atataatttc gttaaaacca gtatctaaaa ttgatggatc ctggttgcca gctatcacct
     1501 tcacactctg gtcaagtttt tgaataacag gcaaaagttc ctcatgtgat tttctatcac
     1561 atactatcac atcaaaacct ttaccaacaa gaaagttggc agaggctaaa cctgtccggt
     1621 aacccagtcc aaccacaagt atgtgtttct tctgctccac agtaactatc ctttacaata
     1681 taaagactac catatatggg cgatagttca tggtaactat cacctgccca ttataaaatc
     1741 ttcagcgaac ttaaacccag tattgccagt ataataccaa taatccacat gcgaaccacc
     1801 accttctgtt ctggccaacc cgaaagttca aagtggtgat ggattggtgc catcttaaaa
     1861 accctttttt ttcgtaattt atatgaagca acctgcacta tgaccgatag tgcctcaatg
     1921 acaaagatgc cgccaacgat gacaaggaaa aactccttct taatcataat ggcaacagtc
     1981 ccaatgatgc cgcccaatgc cagtgacccc gtatcgccca taaatactga tgccggattt
     2041 gagttaaacc acaaaaaccc caagccagca ccacccagtg ctgacaaaaa cactgtcaat
     2101 tccgctgcct gaggtatata ggggatacgc aaatactgcg caataggctg atggccggtg
     2161 agatatgcca ttactgccag cacactaaca acaatagtca ctgtcccaat tgctagccca
     2221 tcaagtccat cagtaaggtt tacagcattg gaactcccaa caataacaag catggcaaat
     2281 agtatccata cccaggacaa atcaagtatt gctctattga taaaaggtac atataaagtt
     2341 gttgcttctt ttacatttga tgggaaaaag taaatacaca gtgaaatcaa aagcccggac
     2401 acacactgca acaaaaattt aagccgtgct ggaatgccat cctttttctt taatattgat
     2461 ttaatgtaat catcaatgaa tccaattgca cctaaaagaa cagtaataac aataagtaaa
     2521 atgaaataca ggttgttaaa attgccccat aaaagcgcgg agataagcac tgcacccaat
     2581 atgagtacgc cacccattgt gggcgttcct gcttttttct ggtgcgattg tggtcccaat
     2641 gtcctgattt cttctttaaa gcgaagatgc gtcagccacc gtataaccaa cccgccaaaa
     2701 atcaatacta ttaaaagcga tgtcaatgca gcatacgccg aacgaaacgt gatatattga
     2761 aacaatcgca gaaacgatag atattcctgt aaaggcaaaa taaagtgata aaacatagtt
     2821 acctcactgt tctaatgcgt ttactacttc ttccatcttc attgaacgcg aacctttcac
     2881 aagcactaca tcatcgtgct tcacaaactg ctgcaagaaa gcaataagct cttctttgga
     2941 atcaaacacg ttaaccagtg aattatctaa tccaccagct atagcacctt ctgcaatata
     3001 ccgtgcatca ttcccccata ctaaaacatg cgaaaaacct gaagccttca atctggttcc
     3061 aactgtaaaa tgcaaatccc ttgaataagt tccaagctct ttcatatcgg ataatacagc
     3121 tatctttcgt ttatcaggga ataccattga agcacttgta aatgcatact ccattgatag
     3181 tgggtttgca ttataacagt catcaatgat agtacagcga ccttttttaa tatcactgcg
     3241 cattgctaca ggtttaaaat tagacaaagc ttcttttact tttataactg atttccccat
     3301 aattctggca actgcaatgg tagctatcgc cgcatataca ttatgaaagc catacgcagg
     3361 cacagtaatt tcaacaccat catattccac tactgtttta tcctgaaaca atgtatacga
     3421 ctctggtgct atatcacctg taagtccaaa ggttataacg tttatatttt tttcacgtgc
     3481 tttcttcttt acaatttcag cgtgctttgt
//
LOCUS       DAOSZP010000190         3503 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_21387_length_3503_cov_4.653422, whole genome shotgun sequence.
ACCESSION   DAOSZP010000190 DAOSZP010000000
VERSION     DAOSZP010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3503)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3503)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3503
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21387_length_3503_cov_4.653422"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(378..1502)
                     /locus_tag="PK348_05185"
     CDS             complement(378..1502)
                     /locus_tag="PK348_05185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65639.1"
                     /translation="MKKLIVVLVIAGVVIAQPLFAATITGTAPQLRVYVGGSWNTIDV
                     STEFNQAILDANAELAKYDDQDKLATGFANANMYSTNAATQQGYPNYSLFAITTGVMV
                     GAQAPTTNVDYYTNGKIEDDIENKGDLYAGVAGSAAVNVGINAGMIIPGLYINVKFGK
                     FDSTWVYDDDDFSFDTMIIGGGITYSLFDEAGTGLLRWRGLTIGTGLVYQTTDVSYRV
                     DLDSKTDSFTVDPGTGTDVTGSVVVDPSFDITLDMYTLSVPFEINTALQFLWLFNINL
                     GVGIDLVSGNADLELQSVSDATVKNIAYGGTPLPENEYIVTPGKVTIDGKTLDKKPSW
                     ARARIMTGIGLNFGAVKIDVPIIYYLKSGAAVGVTAGIIW"
     gene            complement(1835..2977)
                     /locus_tag="PK348_05190"
     CDS             complement(1835..2977)
                     /locus_tag="PK348_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008870728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXK65640.1"
                     /translation="MILERKYDIEEIQKRIRYYPVTAIIGPRQSGKTTLAGMLRPDMI
                     FDLENPVDMAALENPVLQLDKEKGLIIIDEIQLKPELFPVLRYLCDKNPECRFCILGS
                     ASPDIIKQSSESLAGRISFYVLMGFRKEDLPHSLLEHFHFRGGLPRSLLAPDDTTSVM
                     WRKDYVKTFLERDIPLLGIRIPPYTLLRFWQMLAHYHANVVNFSEIGRSLEISDTQVR
                     RYIEILAGTFMVRLLQPWYANIGKRLVKRPKLYIRDSGILHSLLMIESKDALLRHPKL
                     GASWEGFALEAVVRSIKKNDDEIFFYGVHSGAELDMVWQHGGYAWGCEFKFSDAPVLT
                     KSMHNAVQDLQLKKAWIVYPGQKKYKVKNTIEVIPFDDIPDVWVYD"
     gene            complement(3119..3406)
                     /locus_tag="PK348_05195"
     CDS             complement(3119..3406)
                     /locus_tag="PK348_05195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65641.1"
                     /translation="MIDITVIIILVVIATVLLWTVVLFKIVMSPFAIRDKWKLLGVLL
                     IFALCYSILPSWATKTLHLKSYTIPCIIVSILFVGLVILYTMINARLKKYK"
BASE COUNT         1062 a          826 c          561 g         1054 t
ORIGIN      
        1 ttaaatctat cctttcacaa aacaacacga ttcttcactt cgttcagaat gacatgttat
       61 tccactacca tatcgttgtc attacttcgt actaaattag atttcataca ctgttaaaaa
      121 agttattcca ctaccatatc gctgtcatta ccccaacagc agtatccgtt atacaaacaa
      181 aaagaatttg ctaaaaagct tttcccttgt ataccactac catatcgctg ttttacatca
      241 acaccagtac agtctataca aacaaatgag attttgccaa aactttttcc tctattgcat
      301 catcgtatcg ctgtcattcc aacagcagcg ccactataaa ataaaagggg ttttgacaaa
      361 aacccctttt ccctatttta ccatattatc cccgctgtca ccccaacagc cgcaccactt
      421 tttaaataat agataatggg cacgtcaatc ttaaccgctc caaaatttaa accaatccct
      481 gtcataatac gcgcccgtgc ccacgatggc tttttatcca gtgttttacc gtcaatggta
      541 accttgcccg gagtaacgat atactcattt tcaggtaacg gagtaccacc ataagcaatg
      601 tttttaaccg tcgcatcaga caccgattgt aactccagat cggcattacc acttaccaga
      661 tcaatgccaa cacccagatt gatgttgaag agccatagaa attgtaaagc ggtattgatc
      721 tcaaagggaa cgcttaatgt atacatatca agcgtaatgt caaatgaagg gtcaacaacg
      781 acactgccag taacgtcggt acctgtacca gggtccactg taaaggaatc tgtctttgag
      841 tctaaatcaa cccggtatga gacatcggtt gtttgatata ccaagcctgt gccaatggtc
      901 aggccacgcc agcgcaataa gccagtgcct gcttcatcaa acaggctata ggtaatgccg
      961 ccaccaataa tcatggtatc aaagctaaaa tcgtcatcat catataccca ggtactgtca
     1021 aacttaccaa attttacatt gatataaagc cccgggataa tcatccctgc gtttatccct
     1081 acattaacgg ctgcactccc tgcaacacca gcatataaat cccctttgtt ttctatatca
     1141 tcctcaatct tgccattggt ataataatca acattggtag taggtgcctg agcgccaacc
     1201 ataacgccgg tggtgatggc aaacaggcta tagttgggat agccctgctg tgtggcggca
     1261 ttggttgaat acatgttggc attggcaaaa ccagtagcca gtttatcctg atcatcatac
     1321 tttgccaatt cggcattggc atcaagtatg gcttgattaa attctgttga tacatcaatt
     1381 gtgttccatg atccacctac atatacacgt aattgtggtg cagtacctgt aatggttgct
     1441 gcaaacagtg gctgtgcaat aaccacacct gctataacca gcactacaat aagctttttc
     1501 ataagtcccc cttgcaataa aatatttgcc atcgtcactc agaagaagca aagcaacaca
     1561 agaatatgtc gttttagcaa atagattctt cgccttcggc tcaggatgac atacccacca
     1621 ttgtcatttt aataaacacc gtccccaaag aatctattct tttaatacaa caaaacaaga
     1681 ttctttgctt tgactgtcct cgccaccggc attatcgatt aagagtgact atcgcccatg
     1741 gcaaacacca ttcattactt catcttccat tatagttact ttagacatac ctgctttgct
     1801 ttacaaagtg caaaattttt tatatcatta tttgttaatc atatacccat acatccggta
     1861 tatcgtcaaa tggtatcacc tcaatggtat tttttacttt atattttttt tgacccggat
     1921 atactatcca tgcctttttt aactgcaaat cctgtactgc attatgcatt gattttgtca
     1981 gcaccggtgc atcagaaaat ttaaactcac aaccccatgc atatccacca tgttgccata
     2041 ccatatccag ttcagcacca ctgtggacac cataaaagaa aatttcatca tcattctttt
     2101 ttattgagcg cacaaccgct tcaagtgcaa atccttccca tgaggctcct aactttggat
     2161 ggcgcaacaa cgcgtccttg ctttctatca taagaaggct atgcagtata ccggaatctc
     2221 tgatatataa ttttggacgt tttaccaaac gcttcccaat gttggcatac cacggctgta
     2281 ataggcgaac cataaaggta cctgctaaaa tctcaatata acgcctgacc tgcgtatccg
     2341 aaatctctaa tgacctgcct atctctgaaa aattaacaac atttgcatga taatgtgcca
     2401 gcatctgcca gaatcgcaac aaggtatagg gcggaatacg tatgccaagc aatggaatat
     2461 ctcgttctaa aaatgtttta acgtaatctt tacgccacat aacacttgta gtatcatccg
     2521 gagctaacaa tgaacgaggc aatccacctc tgaagtgaaa atgctccagc aaactgtgtg
     2581 gtaaatcttc tttacgaaat cccatgagca cataaaatga tattcttccc gccaatgact
     2641 ccgatgattg tttgattata tcaggagaag cacttcctaa tatacaaaac ctgcattcag
     2701 gatttttatc gcacagatat cgcaataccg ggaaaagttc tggttttagc tgtatttcat
     2761 ctatgattat caatcccttt tctttatcaa gttgtaaaac aggattttca agggcagcca
     2821 tatccacagg attttccaga tcaaatatca tatcaggtcg taacatccct gccaatgttg
     2881 ttttacctga ttgccgtgga ccaataatgg ctgttacagg gtaatagcgt atgcgttttt
     2941 gtatttcttc aatatcatat tttctttcta aaatcatcgt ttccttgaaa tttctttatg
     3001 agttgctgat ttatcatggt attctacccg ggtattggat acctgtcaac caataatcgc
     3061 attatccgcg ttttggaatt ggcaaaacca tgttacctat aaactatcta tcgcaaccct
     3121 atttgtattt ttttaaccgt gcattgatca ttgtgtacag tatcacaagc ccaacaaaaa
     3181 gaatgctcac aatgatacag ggtatagtat agcttttgag gtgcaatgtt tttgttgccc
     3241 acgacggcag gatactataa catagcgcaa aaatgagcag cacacccaat agcttccatt
     3301 tgtccctgat tgcaaacggg ctcataacaa ttttaaatag caccaccgtc cacaaaagaa
     3361 ctgtggcaat aacaaccaat attattatga cggttatatc aatcatagca aatcccttta
     3421 tgcgttattc tggtgatatt cattaaaccc atgatttttt aaatacatca caattgcgtc
     3481 acggtatttc ataaaatctt ctc
//
LOCUS       DAOSZP010000191         3503 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_21390_length_3503_cov_3.569316, whole genome shotgun sequence.
ACCESSION   DAOSZP010000191 DAOSZP010000000
VERSION     DAOSZP010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3503)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3503)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3503
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21390_length_3503_cov_3.569316"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            193..1140
                     /locus_tag="PK348_05200"
     CDS             193..1140
                     /locus_tag="PK348_05200"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HXK65642.1"
                     /translation="MSGLQRKDLLDIQSLATEEILLICNSAKYFKALFTRSIKTVPVL
                     RGKTVCLLFYEPSTRTRISFELAAKRLSADLINIAVQTSSVVKGESLIDTVHTLEAYK
                     ADFIVMRHAASLAPHFLSRNIKSSVINAGDGNHAHPTQALLDAYSLMEIVGSIKGLHI
                     GIVGDILHSRVARSDIEIFKRLGAKVSLIGPPTLVPDEFKMYGVDIYYEFDEILPELD
                     VINMLRIQRERQQGSFFPSIREYHKRFALTNERLKRCKKDVIVMHPGPINRGIEIDDE
                     VADSSHSIINEQVTNGVAVRMAIFYLLAGGTPLDEKEVE"
     gene            1140..2177
                     /locus_tag="PK348_05205"
                     /pseudo
     CDS             1140..2177
                     /locus_tag="PK348_05205"
                     /inference="COORDINATES: protein motif:HMM:TIGR00857.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
     gene            2216..2413
                     /locus_tag="PK348_05210"
     CDS             2216..2413
                     /locus_tag="PK348_05210"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65643.1"
                     /translation="MLGIDRGYIKEGGVADITIINPDKKINVNDDFIISRCKNTPFMG
                     RELFGQVEYTICNGIIVYPFS"
     gene            complement(2406..3011)
                     /locus_tag="PK348_05215"
     CDS             complement(2406..3011)
                     /locus_tag="PK348_05215"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HXK65644.1"
                     /translation="MIKPLKTIEKLKNGQAVTIVALGDSLTYGWMVDKGYIDYFHDFL
                     QSQYPNASIKIINKGIPGDTADGGLHRVSYDVISYNPDCVLIQFALNDYACGYSPHVF
                     GNYIRAIINAIQARLPSDIVLVTSVWFGDFPEAKNAYKLYDTILSIADDYKLPVSKTH
                     EYWKNAVNKGTPLQELVQFDGVHPTEEGYYIMARALQDLFS"
     gene            3170..>3503
                     /locus_tag="PK348_05220"
     CDS             3170..>3503
                     /locus_tag="PK348_05220"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXK65645.1"
                     /translation="MDLNNKCILLTGASSGIGKALLEELTQYNATIVVGDLNPEAIAH
                     NKNIKAVCCDVSKQKDIEMLFKKALSIMGEIDICIANAGFAYYEKLQKADYDHIHKIV
                     SVNYIAPIY"
BASE COUNT         1116 a          604 c          735 g         1048 t
ORIGIN      
        1 cttcttgaac agatcctcgt cttaccgcaa ctattttgac ttttttatca ttcctccttc
       61 tttacaatat acacacaggc tacaagtgtg cagtacatat ataatagttc aatcaaaaat
      121 atatttaaaa aaattattga aattatttat gccatttggg atgatatata acaagaaaag
      181 gtaatacagg atatgagtgg tttacaaagg aaagacttac ttgatataca atcattagct
      241 accgaggaaa ttttattaat ttgtaattct gcaaaatatt tcaaagccct ttttacacgg
      301 tcaattaaaa cagtgccagt tttacgcggt aagacggtgt gccttttatt ctatgagccc
      361 tcaacacgca cccgtataag ttttgagctg gcggcaaaac gtctgtcagc agatttaatc
      421 aatattgcgg tgcaaacatc aagtgtggtt aaaggggaat ccctgattga tactgtgcat
      481 acgctggaag cgtataaagc agattttatt gttatgcgac atgcggcgtc attagcgccg
      541 cattttttat ctcgtaatat aaaatcatcg gttattaatg caggagatgg caaccatgct
      601 catccaacgc aggctcttct tgatgcgtat tcactgatgg aaatagtggg cagtatcaaa
      661 gggttgcaca taggtatagt gggtgatatt cttcattcac gtgttgctcg ttctgatatt
      721 gagattttta agcggctcgg agcaaaggtt agcctgattg gccccccaac gctggtgcct
      781 gatgaattca agatgtatgg tgttgatatt tattatgagt ttgatgaaat acttcctgaa
      841 cttgatgtta ttaacatgtt acgaatacaa cgtgaacgcc agcaggggtc atttttccct
      901 tcaatacggg aataccataa gcgctttgcg ttaactaatg aacggcttaa acgatgtaag
      961 aaagatgtga ttgtgatgca tcctggacca attaaccgcg gtattgaaat agacgatgaa
     1021 gtggccgata gttcccattc aataataaat gagcaggtca ctaatggggt agcagtgagg
     1081 atggctattt tttatcttct tgcaggtgga actcccctgg atgagaaaga ggttgagtaa
     1141 tgaaattgtt aattaaatat ggaagattaa ttgaccctgc ttctaacact gatgaagaat
     1201 ttgatatttt tattgcagat ggcaaaatag taaaaataga taagaatatt aaaaaggatg
     1261 atgcacagtt cattaatgca aaggattgta ttgtggttcc tggactggtg gatatgcatg
     1321 tacatttcag agagcccggc cgtgaggatg ttgaaactat tataggtggc tccagggttg
     1381 cagctaaatc agggtacacc agtgtatgta ccatgccaaa tacaaatcct gtaattgata
     1441 atcaggcgct ggtgcgatac ataaaaaaac aggcagaatc tggaccaatt aatgtatttc
     1501 cagttgcagc cataacaaaa ggttcaatgg gtgaagaatt aacggagatg ggtgagctgg
     1561 ttgaaggtgg tgcaatagca ttcagcgatg atggcaaacc cgttatgaac tcaactacaa
     1621 tgcgacgtgc attagagtat tcgcggatgt ttaatgtccc tattataacg catgcagagg
     1681 atatcacatt gtcaagcggt ggtatcatga acgaaggaaa caattcaatc tttttgggtt
     1741 tgaaaggaat cccccgtgaa gctgaagaaa ttatgattgc gcgcgatgta ttgcttacac
     1801 gcctcacaaa agggaggttg catgtagctc atgtttcatc taaaggttca ctggatatta
     1861 ttaccatggc caaacgaaac aacattcagg tgacgtgtga gacagcaccg cattattttt
     1921 ctctgacgga tgatgccata gcggaacatc tttccatggc caagatgaat cctccattgc
     1981 gtacggaaga agatagaaag gcaattattg aagcgttgcg caatggctta atagaagtca
     2041 ttgccacaga ccatgcgcct catcttccca atgaaaagat gcaggaaata gcatttgcac
     2101 catttggaat tataggtctt gaaactgcag tgccattaat tattactgtc ttattaaggg
     2161 gaatgggttt aactttatac aggcatttga aaaagttact gtcaatccct gtaagattct
     2221 tggcattgac agagggtata tcaaagaagg tggagttgct gatattacga ttatcaatcc
     2281 tgataaaaaa attaatgtta atgacgattt tattatttcg cgatgtaaga atacgccatt
     2341 tatgggaaga gagctttttg gccaggttga atataccatc tgcaatggta taatagtata
     2401 tccgtttagc tgaataaatc ctgtaatgca cgtgccatga tatagtagcc ttcttcggtt
     2461 gggtgtacac catcaaactg tacaagttcc tgtaatggtg tgcccttgtt aacagcgttt
     2521 ttccagtatt catgtgtctt tgatacaggc agcttatagt catcagcaat agatagtatt
     2581 gtatcgtata atttatatgc attttttgct tcagggaaat ccccaaacca cacagaagta
     2641 accagcacta tatcagatgg caatcgggcc tgaatagcat tgattattgc ccgtatgtag
     2701 ttaccaaaaa catgcggtga atacccacat gcgtaatcat tcaatgcaaa ctgtataagc
     2761 acacaatcgg gattgtacga aatgacatca tacgaaacac gatgcaaccc gccgtcagct
     2821 gtgtcgcctg gtataccttt attgattatt tttattgaag catttggata ctgtgattgt
     2881 aaaaaatcat ggaaataatc aatatatccc ttatcaacca tccatccata ggttaatgaa
     2941 tcacctaaag caactatggt gacagcctgg ccgtttttaa gtttttcaat agttttcagt
     3001 ggcttaatca tgacactacc acaaattcct caaagattgt aaagactgta tgaaaattgt
     3061 caattagcaa aattgtaatg ggtttactgt agcaatgaaa tattcttgca atattagagt
     3121 tctactatat tttaagataa atttgaaaaa tcttgcagag aggtacagga tggatctaaa
     3181 caataaatgc atactattaa caggtgcatc atccgggata gggaaagcat tgctggaaga
     3241 attaacacaa tacaatgcaa caattgtggt tggcgatcta aaccctgaag ctattgcaca
     3301 caataaaaat ataaaagcag tatgctgtga tgttagtaag cagaaagata ttgaaatgtt
     3361 gtttaagaag gcactttcca ttatggggga aatagatatc tgcattgcga atgctggttt
     3421 tgcctattat gaaaaattgc aaaaagctga ctacgatcat atccataaaa tagtttcagt
     3481 aaattatatt gccccaattt att
//
LOCUS       DAOSZP010000192         3492 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_21458_length_3492_cov_3.425371, whole genome shotgun sequence.
ACCESSION   DAOSZP010000192 DAOSZP010000000
VERSION     DAOSZP010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3492
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21458_length_3492_cov_3.425371"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="PK348_05225"
     CDS             <1..399
                     /locus_tag="PK348_05225"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXK65646.1"
                     /translation="NCVLLSSLAGLIGVYSYSAYGTSKFAVFGLADCLRSEFKENGIA
                     LSVICPPEVDTPFLQAELATIPATAKRIKKITGVLSADTIAKAILKAVQKNTYMVVPG
                     FMGKLTWLLHRLGNGFGTRLITDIIVRYSK"
     gene            418..1254
                     /locus_tag="PK348_05230"
     CDS             418..1254
                     /locus_tag="PK348_05230"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HXK65647.1"
                     /translation="MGKCSCKFDNVLIGISFVSVLLVSLYIGVYYKTDTYIENEKEVY
                     RAIAHKNITRLHKVPILLYHNINGKGPYSLPFDKLKNQFTQLQNNGIKVISLQQLESH
                     IDEHNPFGEKVISITFDDGYYSMYTMLMPMARSMSYPVTLFVYIDAIAMGGTKNLTWK
                     KLQIMQNNNIDIQCHSHSHADLVKLYNQGTLESRYALYREIYLSKKILELYLHKNIDY
                     FAFPYGRYSIELTELCKDAGYKRVFSTDYGPNIVTRDNFCLRRHHVKSNYSDEKVLSL
                     AW"
     gene            1403..2125
                     /locus_tag="PK348_05235"
     CDS             1403..2125
                     /locus_tag="PK348_05235"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HXK65648.1"
                     /translation="MGIKKIVLVFFVIVTTVTFANELYFIDNEHIQRYNGKSGKWIEV
                     STLKQLKAYAEQFGTSIDEVYVINSINEKKFKKSYVFVPYSDAYIQMLMEKGISLQPV
                     NISYNEFIWPIGDPEKITSGLGRRWGRFHPGVDIPAPKGTLVRAAMEGKVIYAGYCGN
                     YGNSITLEHRDNYITRYAHNSVLLVKVGDYVQKGQVIALVGSTGRSTGNHLHFEIRCA
                     DIPLNPLDILPEKNLIIMKNPK"
     gene            2143..3027
                     /locus_tag="PK348_05240"
     CDS             2143..3027
                     /locus_tag="PK348_05240"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HXK65649.1"
                     /translation="MAHRIDLHIHSHYSEDGDYSVDYLFELAAKKGLSAISITDHDSI
                     ESIPDALNASNIYGVEYIPGVELTTIYDDGSQQHILGYYIHENPQLNATLKQIGQFRW
                     EIAKKRIKKLQEIGFSMNEDNVWQKANNRPPTATAIMLEVFENPENRNDKRLQEYFEG
                     KKSDNRLMFFYREYLSENMPAYVPFISIPTKEGIDVIKSSGGIPVLAHPKFVNGEEHL
                     RNIVSMGIMGIEAISSYHNEHDIAFFMEFAVQHNLVITAGSDFHGPTSKPKVSLGGIK
                     DNDYSLLEELKKLHKKYS"
     gene            complement(3078..>3492)
                     /locus_tag="PK348_05245"
     CDS             complement(3078..>3492)
                     /locus_tag="PK348_05245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65650.1"
                     /translation="FKKLMVDRKESGLIVTYTPLPVPAYLNAPETPTKYKITVSGKTI
                     DEKLYYAEDSDGDGITETFMVNSADGFNWGYKSGANIIFIYNNLDEEIKGLIGQLCNW
                     AYYGTPEEEKEILQNFPKDSDIINEFKLEVPQTTK"
BASE COUNT         1128 a          575 c          692 g         1097 t
ORIGIN      
        1 aactgtgtat tactgtcatc acttgcgggg cttattgggg tatatagcta ttcggcgtat
       61 ggcacatcaa aatttgccgt gtttggactt gccgattgcc tgcgcagcga gtttaaggaa
      121 aacgggattg ccctttcggt aatttgcccg ccggaggtgg atacaccatt tttacaggcg
      181 gagcttgcaa cgattcctgc cacggcaaaa cgcattaaaa aaattacagg ggttttatca
      241 gcagatacta tcgccaaagc tattttaaag gctgttcaaa agaatacgta tatggttgtg
      301 ccggggttta tgggtaaact gacatggttg ctgcaccggt taggtaacgg gtttggtacc
      361 cgccttatta cggatataat cgtcagatat tcaaaataat atgatacagc gtgcataatg
      421 ggaaagtgct catgtaagtt tgataatgta ctgattggta ttagttttgt aagtgttctt
      481 ctggtgtcat tgtatatagg tgtatactat aaaactgata catatataga aaatgaaaaa
      541 gaagtttaca gggcgatagc tcataaaaat attacccggc ttcataaagt tccaatactt
      601 ttataccata acatcaatgg caaaggcccc tattctcttc cttttgataa gctgaagaac
      661 cagtttacac aattgcagaa caatggtatt aaggtaatat cactgcaaca actggaatcg
      721 catattgatg agcataatcc atttggtgaa aaggttatat caataacctt tgatgatggc
      781 tattactcca tgtataccat gttaatgcca atggcacgtt ccatgagcta tcctgttacc
      841 ctttttgtat atatagatgc tatcgcgatg ggcggaacaa aaaatttaac atggaagaag
      901 ttgcaaataa tgcagaacaa taacatagac atacaatgcc attcacatag tcatgctgat
      961 ttggtaaaac tatataacca gggaacactt gaaagcagat atgcattata cagggagatt
     1021 tatctttcaa aaaagattct tgagttatat ttacataaaa atattgatta ttttgcattc
     1081 ccttatggcc gctattcaat tgaattaaca gagttgtgta aagatgcagg gtataaacgg
     1141 gtgttctcta ctgattatgg ccccaatata gttacaagag ataatttttg tttacggagg
     1201 caccatgtta aaagcaatta ttcagatgaa aaagttttat cgttagcatg gtaaaaaaat
     1261 tgttgataat tagtcagtaa aattcaagct ataataaggt tactgtttgc ttataaataa
     1321 ttttctatac atataatctc tatagtaagc taactcccag agtggttgtt gtaaaaaatg
     1381 atatgtacag gattgatctg ttatgggtat aaagaaaata gtacttgttt tttttgttat
     1441 agttaccaca gtaacatttg ccaatgagct ctatttcata gataatgaac atatccagcg
     1501 gtataacggg aaaagtggta aatggataga agttagcacc ctcaaacagc taaaggctta
     1561 cgctgaacag tttggtacgt ctattgatga ggtatatgta ataaatagta ttaatgaaaa
     1621 aaagtttaaa aaatcatatg tttttgtccc ttattcggat gcttatattc aaatgttgat
     1681 ggaaaaaggt atctccttgc agcctgtaaa catttcatat aatgaattta tatggccaat
     1741 tggcgatcct gaaaaaatta catcaggcct tggtcgacgc tggggaagat ttcatcctgg
     1801 agtagatatt ccggctccta agggaacact tgtacgggca gccatggaag ggaaagtaat
     1861 ctatgctgga tattgcggga actatggaaa ttctataaca cttgaacaca gggataatta
     1921 tatcacccgg tatgcccata attcggtgtt acttgtaaaa gttggtgatt atgttcagaa
     1981 aggccaggtc attgcccttg taggaagtac gggccgttct accggcaatc atttacattt
     2041 tgagattcgt tgtgctgata taccgctgaa tccacttgat atactgcctg aaaaaaatct
     2101 cattatcatg aaaaacccaa aataatgtga tacaattttt gtatggctca ccgcattgac
     2161 ctgcatatac attctcacta tagcgaggat ggtgattatt ctgtagatta tctttttgaa
     2221 cttgctgcaa agaaaggttt atccgcaata agtattaccg atcatgattc aatagaatca
     2281 atcccggatg cgctgaatgc ttcaaacata tatggagtag aatacatccc gggtgttgag
     2341 ttaaccacaa tatatgacga tggttcacag caacatattc tgggttatta tatacatgaa
     2401 aatccacaat taaacgcaac attgaaacaa atagggcaat tcaggtggga gatagcaaaa
     2461 aaaagaatta aaaagttgca agaaattggt ttttctatga atgaagataa tgtgtggcaa
     2521 aaagcaaata accgtccccc aactgctaca gctattatgc tggaagtgtt tgaaaatcct
     2581 gaaaacagaa atgataagag attacaggaa tattttgaag gaaaaaaatc tgataataga
     2641 ctaatgtttt tctacagaga atacctttct gaaaatatgc ctgcctatgt accattcatt
     2701 tccatcccaa caaaagaagg gattgatgtc attaagtctt caggtggtat accggtatta
     2761 gctcacccaa agtttgtaaa tggggaagag catttacgta acattgtttc gatggggata
     2821 atggggatag aggctatctc ttcatatcac aatgagcatg atatagcatt ttttatggaa
     2881 tttgctgtac aacataacct tgttataact gcaggatctg atttccacgg tccaacatcc
     2941 aaaccaaaag tttccctggg tggtataaaa gataatgatt acagtttact ggaagaattg
     3001 aaaaaattgc acaaaaaata tagttgaaca ggggtaacat gtaggggcga gttttaaatt
     3061 cgcccctaca tgtttgttta ttttgttgtt tgtggtacct ctaatttgaa ttcatttatg
     3121 atatcgctgt ctttggggaa attttgtaaa atttcttttt cttcttccgg tgtgccataa
     3181 tatgcccagt tacatagctg gccaattaaa cccttaattt cttcatccag attgttatat
     3241 ataaaaataa tattggcacc tgatttatat ccccagttaa agccatcagc actattgacc
     3301 atgaaggttt cggtaatgcc atcgccatca ctatcttcag catagtatag cttttcatca
     3361 atagtcttgc cacttacggt tattttatat tttgttggtg tttcaggtgc attgaggtat
     3421 gcaggaacag gaagtggtgt ataggttaca ataagacctg attcctttcg gtctaccata
     3481 agttttttaa ag
//
LOCUS       DAOSZP010000193         3492 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_21459_length_3492_cov_3.194356, whole genome shotgun sequence.
ACCESSION   DAOSZP010000193 DAOSZP010000000
VERSION     DAOSZP010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3492
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21459_length_3492_cov_3.194356"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(59..616)
                     /locus_tag="PK348_05250"
     CDS             complement(59..616)
                     /locus_tag="PK348_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HXK65651.1"
                     /translation="MKKLSYEEIKLHRPDTKELFRLQRFPVVTVLENIRSMYNVGSMF
                     RTADAARIAYMYLCGYTPYPPRKEIEKTALGSTESVPWEHSIKPVSVLRMLKQQGFTI
                     AALEHTTKSISYFSYHYTYPLCIVVGNEVEGISSELLSLCDVALEIPMFGIKHSLNVA
                     VAYGIVVFHAVMAYQSEGKISESSD"
     gene            complement(632..1648)
                     /locus_tag="PK348_05255"
     CDS             complement(632..1648)
                     /locus_tag="PK348_05255"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HXK65652.1"
                     /translation="MKKCIVVLLVLISGCSSLNGVSHTHETNLMESTQNPYQIANKLE
                     FSEVWAYLMKGEEDKIKGDEPITDLCYFSAKVSGKGQITGEYVPPSIKFSNKIRHHIV
                     IAVLNNFALMHFVLNPDLPVRKKFINDICTVASKFDGVQIDFESVASSDASWFFSFLT
                     ELRSALEKDKMLSIALPPRRTRIADAYDYPSIAAVADRVIIMAYDQHWSGSNPGPIAG
                     LSWCREVAEFAKSFIPQEKLIMGLPLYGRSWQDKNFNKSVHYHNVEEMIKASNIQPQY
                     DPENGAYFEYEEKVKIRVYYTDERAIYDRLKLYSSMSIDSVAFWRIGQGPRGMWQGIS
                     SKSL"
     gene            complement(1921..2289)
                     /locus_tag="PK348_05260"
     CDS             complement(1921..2289)
                     /locus_tag="PK348_05260"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXK65653.1"
                     /translation="MNPILIVYENDRFAQLIQFVLTTAGYQIVKSNDPLELFEKVKEL
                     KPPLIVMDVFFKHIDGLYLLERLYGIKEFAPIPVFIVSSRNEPLTLFDALERGAADYL
                     AAPINEDMLIDKVNKLLKGN"
     gene            complement(2298..>3492)
                     /locus_tag="PK348_05265"
     CDS             complement(2298..>3492)
                     /locus_tag="PK348_05265"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HXK65654.1"
                     /translation="DKLLTFDVLDQNGNPVFSAGQALSDEVLKKKSEEGVRFFIPRVT
                     ETIGVYDREIIDMKKLHAIADDTATMYTDIRKSGRMSYEQFLQSHNQLAAIIDSLRAE
                     DKTGGVLSLLKDIKDFDYITYTHSVNVGVLAMILVYKELSTGDKVKLAALAGYLHDIG
                     KLRVEQAIIQKPGLLSYDEFKKVLEHTREGYRILDSVVDSSGRKVVPNIIKYVALFHH
                     RKLQSPGYPYRDDDKDKTRESQYNEIPDLARIMGICDMYDALTSATPFRMPMTVEKAL
                     RYILNLSNYLYSTDDVHRFLKVLGLSLNKGNPFLRIGDFIMLESEKVSLDTRKKIRVY
                     EIARIEEISRINFLNPKVMIFYDVLRNKKLNNIIVDLRYDTSRRIVRVFESTRIREML
                     LRQLK"
BASE COUNT         1061 a          744 c          580 g         1107 t
ORIGIN      
        1 ttccattttt cttttccttt ttttaagatt accttttgca ttatgttgta tgtacacatt
       61 aatcagatga ttctgaaatc ttcccttctg attgatacgc cataactgca tgaaatacca
      121 ctataccata cgccactgca acattaagtg aatgctttat accaaacata gggatctcca
      181 gtgccacatc acagagcgac aacagttcac ttgagatacc ttccacttca ttgccaacca
      241 caatacacag cggataggtg taatgatatg aaaagtaaga gatgcttttt gtggtatgct
      301 ccagtgcagc aatggtaaac ccctgctgtt ttaacatccg taacacagat acaggcttaa
      361 ttgagtgttc ccaaggcaca ctttcggtac tgcctaaggc tgttttttca atttcttttc
      421 gtggcggata cggagtatac ccgcacagat acatatatgc aatgcgtgct gcatcagcgg
      481 ttctgaacat tgaaccaaca ttatacatgc tacgtatatt ttccagtact gtaacaaccg
      541 ggaaacgttg taacctgaat aactcctttg tatcagggcg atgtagtttt atttcttcgt
      601 aggataattt tttcattttt taacagaatc actacaggct ttttgaactg attccctgcc
      661 acatccccct tgggccctgc cctattcgcc agaaagcaac actgtcaatt gacattgatg
      721 aatacagttt aagacgatca tatattgccc tttcatctgt ataatacaca cgtattttta
      781 ccttttcttc atactcaaag taagctccat tttcaggatc atactgtggt tgtatattgg
      841 aagcttttat catttcttca acattatgat aatgtaccga cttgttgaaa tttttatcct
      901 gccacgatct tccataaagt gggagcccca tgattagctt ctcctgcggt ataaaacttt
      961 ttgcaaattc agctacttcc ctgcaccatg ataatccagc tattggacca gggttacttc
     1021 ctgaccagtg ctggtcataa gccataatga tgactctatc agctactgct gcaatggaag
     1081 ggtaatcata agcatcggct attcttgttc tgcgtggtgg gagggcaatt gaaagcattt
     1141 tatctttttc cagagcagaa cgcaattcgg ttaaaaagct aaaaaaccat gaagcatccg
     1201 atgaagcaac cgattcaaaa tctatctgca caccatcaaa ttttgaagct actgtgcata
     1261 tgtcattaat aaactttttg cgaacaggca aatcaggatt aagtacaaaa tgcataagtg
     1321 caaaattatt aagaacagct ataacgatat gatgccgtat tttattgcta aactttattg
     1381 aaggtggtac atattcgccg gttatctgcc ctttgccact tacttttgca ctgaaataac
     1441 aaaggtcagt aattggctca tcacctttga ttttatcttc ttcacctttc atgaggtatg
     1501 cccacacttc actaaactcc aatttattag ctatctggta tgggttttgg gtagattcca
     1561 taaggtttgt ttcatgtgta tgtgatacac catttaatga ggaacatcct gatataagca
     1621 ccaaaagcac aacaatacat ttcttcattg ccgattcttc ctaaaataat aaaattttca
     1681 ataaagtcat gccaatctca attttggtat agcaaagatt tttcaaatga atagtcattc
     1741 ctggcaacgt gactgtgacc ctgatgcaat cgaaacctgc aatcgcaact ttaaaacatt
     1801 agattacttc atcgtcccaa tattcagaaa ctgctcacaa atatgaaatt tcctcattaa
     1861 ttgatatgag acgctactat atttgtcgga tggaattatt tcaaaacaac agtgtttgtt
     1921 ttagtttcct ttcaataatt tattcacttt atcaatcagc atatcttcat ttatgggtgc
     1981 agctaaataa tcagctgcac cacgctcaag agcatcaaaa agtgtgagtg gttcattacg
     2041 ggaagaaaca atgaagacag gaataggtgc aaactcctta attccgtaca accgttctaa
     2101 taaatataat ccatcaatat gtttaaaaaa cacatccata acgataagtg gaggttttaa
     2161 ctctttgact ttttcaaata attccaacgg gtcattactc ttaacaatct ggtagccggc
     2221 agttgttagt acaaactgaa taagctgagc aaatctgtca ttttcatata caatcaatat
     2281 tggattcata cgttcaccta tttgagctgc cgtaacagca tttcccggat tcgtgtactt
     2341 tcaaaaactc gaacaatcct gcgacttgta tcatatcgca aatctacaat tatattatta
     2401 agctttttat ttcgtaatac atcataaaaa atcattactt tgggattcaa aaaattaata
     2461 cgtgaaatct cttcaatacg ggctatttca tagacacgta tctttttacg ggtatcaagg
     2521 cttacctttt cagattcaag catgataaaa tcgccaattc ttaaaaacgg atttccttta
     2581 ttgagcgata gccctaaaac cttaaggaag cgatgaacat catcagttga atagaggtaa
     2641 tttgaaagat ttaatatata gcgcaatgcc ttttctactg tcattggcat acggaacggt
     2701 gttgcactgg taagggcatc atacatatca caaataccca taatacgtgc aaggtcgggt
     2761 atctcattat actgcgattc acgtgttttg tccttatcat catcacggta aggatacccg
     2821 gggctttgaa gcttgcgatg atggaatagt gccacatatt ttataatatt aggtactact
     2881 ttccgtcctg atgaatccac tacactatca aggatgcgat acccttcacg ggtatgttcc
     2941 agtacttttt taaattcatc atatgaaagc aatcccggct tttgaatgat ggcctgttca
     3001 acacgcaatt tcccaatatc atgtaaataa cccgccagag cagctaattt aactttgtct
     3061 cctgttgaaa gctccttata taccaatatc atcgccagta caccaacatt gaccgaatgc
     3121 gtataggtga tataatcaaa atccttgata tccttcagca atgataacac acctcctgtc
     3181 ttatcctcag ccctcaggct atcaataata gctgccaatt gattatgtga ttgcaaaaac
     3241 tgctcatacg acatccgccc tgatttacgg atatctgtat acattgttgc agtatcatca
     3301 gcaatggcat gcagcttctt catatcaatg atctcgcggt catagacacc aattgtttca
     3361 gtaacacggg ggatgaaaaa ccgcacaccc tcttcggatt tcttttttaa aacttcatca
     3421 ctaagtgcct ggccagctga gaaaacagga ttgccattct ggtccagtac atcaaaggta
     3481 agcaacttat cg
//
LOCUS       DAOSZP010000194         3491 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_21468_length_3491_cov_3.557334, whole genome shotgun sequence.
ACCESSION   DAOSZP010000194 DAOSZP010000000
VERSION     DAOSZP010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3491)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3491)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3491
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21468_length_3491_cov_3.557334"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            577..2163
                     /locus_tag="PK348_05270"
     CDS             577..2163
                     /locus_tag="PK348_05270"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005998085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HXK65655.1"
                     /translation="MTVTKSKQNKVFIFDTTLRDGEQAPGFSMNIDEKLHFADQLVKL
                     GVDIIEAGFPISSPGDFEAVKRISERVKGVQVAGLARANFKDIDVAWDALKYADNPRI
                     HTFISSSDIHIQYQFKKTREEVLAIAVDAVKHAAKYTSNVEFSPMDATRSDWSYVAQM
                     VEAVIKAGATTVNIPDTVGYAIPSEFHEFIKFLFNTVPNIDKAIISVHCHNDLGLATA
                     NALAAIEAGARQVECTINGIGERAGNTAMEELVMALKTRGEKFQVYTDINSKQIMSTS
                     KLLTHITGVSVQPNKAIVGANAFAHESGIHQDGVLKNAMTYEIMKPEDVGITKSTLVL
                     GKHSGRHAVIARLNSLGYNLTDEELERFFKLFKVLADKKKQIFDEDLEVLVGEAVYKM
                     KGRYKVTNVQISTGMYSPPMTLVTIKDRYNDGVETFDVAHGNGGVDAGINAVKKITGT
                     SAKIESFNLVAITGGSDALCEVTVTVVEEINGRQLKVFGNGVNIDISVAGILSFVDAL
                     NKLEYIKQRNHIGEKIEAKL"
     gene            2179..>3491
                     /locus_tag="PK348_05275"
     CDS             2179..>3491
                     /locus_tag="PK348_05275"
                     /inference="COORDINATES: protein motif:HMM:TIGR03904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HXK65656.1"
                     /translation="MPVTKEEALKLSWNNVDIVLVTGDAYVDHPSFGIAVIGRVLEKA
                     GFSVGIIAMPNFLDPSSVEVFGRPNLFFGVTSGNIDSMLSRFTAFKKIRNDDPYIPGG
                     KGGIKPSNAVITYCNLIKQRFKDVPIVIGGIEASMRRLLHYDFVQDKLRRSIMVDSRA
                     DILVYGMGEYQILEIARRLSGGQSLQGIPGTVTIAKQKPDDAVELPTEEDALANVESL
                     LVMYKMFYQNFGTKVIAQKSGGRYIIHTPPPAVTRKELDAYYELPYERKPHPMYKDTI
                     PAFEMIKYSINAHRGCAGGCSFCSLTLHQGKMVVSRSADSIKQEIVTVSKRKVHISDI
                     GGPSANMYGFTCAIGGCNRESCVFPAVCKHLRLATDKWLNLMEDALTLPNVKKVSVGS
                     GIRYDMFMQGSNAKKNLEFLVKHFISGQLKIAPEHTDKAVLKAMR"
BASE COUNT         1084 a          611 c          828 g          968 t
ORIGIN      
        1 tttccccttg tgaagagaat attatggaat tgttgccgtc tggacaaaag aaagtgcagg
       61 gaagggcagg gagtatcctg ggttactcaa ggtggatagt atccgaatgg gataacaata
      121 tatggatttt ttgtctgtga aaatcaagca tagataccat tgtatggaag aataataaaa
      181 gtgtatttta ttactggctt caagccgaaa taaagtatta gagtattaaa attttgttat
      241 ggaatgatta aaatatattg acaacatagt attaggtgat ttatttgaat taaatggtaa
      301 tgtatataat gatatgacat ctattatgat ttcacgaaac ctttacaacg tagctaacct
      361 tctaggtcta ctactattgg ggaaaaccgc aacaggggtt tcgtgagagg tattggtata
      421 gtgaaatgtt atgccgccac ggaaaccccg tggcggtttt attttatatg cactggaggt
      481 gtaaagggat gaattcccaa cgattactaa aaaaattatt accaagaaat tatcgctatt
      541 aattttgata tcaactacag gtaaaggaga aaggccatga cagttacaaa atcaaaacaa
      601 aataaagtat ttatatttga tacaacatta cgtgatggtg agcaggcgcc aggcttcagt
      661 atgaatattg atgaaaaact tcattttgct gaccagcttg taaaattggg ggtggacatt
      721 attgaagcag gattcccaat atcctcgccg ggtgattttg aagccgtaaa gcgcatatca
      781 gaaagagtaa aaggagtgca ggtggcaggg cttgcacgtg ctaacttcaa agacattgat
      841 gtggcgtggg atgcattgaa gtatgcagac aatccacgca tccatacctt tatttcaagc
      901 tcagatattc atattcaata tcagtttaaa aagactcgcg aagaggtgct ggcgatagct
      961 gttgatgctg taaagcatgc tgcaaaatat acctcaaatg ttgaattttc cccaatggat
     1021 gccacccgaa gtgactggag ctatgtagcg cagatggtgg aagcagttat taaagctggt
     1081 gccacaacag tgaacattcc agatacggtg gggtatgcaa ttccatcgga gtttcatgag
     1141 tttataaaat ttctttttaa taccgtacca aatattgata aggcaatcat atcagtgcat
     1201 tgccataatg acttaggact tgccacagca aatgcattag ctgctataga agctggtgca
     1261 cgacaggtgg agtgtaccat caatggcatt ggtgagcgtg caggcaacac tgccatggaa
     1321 gaactggtaa tggcattgaa aacacgtggt gaaaaatttc aggtgtatac tgatatcaac
     1381 tcaaagcaaa ttatgtcaac atccaaactt ttgacgcata ttacgggggt gagtgtgcag
     1441 ccaaataagg caattgttgg tgctaatgcg tttgcccatg aatcaggcat acatcaggat
     1501 ggtgtactta aaaacgcaat gacctatgaa ataatgaaac ctgaagatgt aggaattacc
     1561 aaatcaacac tggtactggg caaacattcg ggtcgccatg cagttattgc acggttaaac
     1621 agtttagggt ataacctcac cgatgaagaa ctggaacgtt tctttaaact cttcaaggtg
     1681 cttgctgata aaaagaaaca gatatttgat gaagacctgg aagtgctggt aggtgaagca
     1741 gtatacaaga tgaaagggcg ctacaaagtt accaatgtac agatttcaac tggtatgtat
     1801 tcgccaccaa tgacactggt aactattaaa gaccgctaca atgatggtgt tgaaacattt
     1861 gatgtggcac atggcaatgg tggtgttgat gcaggtatca atgccgttaa aaagattacc
     1921 ggtacatcag caaagattga gtccttcaat ttagttgcta tcaccggcgg atcagatgca
     1981 ttgtgtgagg taactgtaac agtggttgaa gaaataaacg ggcgacagct caaagtattt
     2041 ggcaatggtg tcaatattga tatttcagtg gcaggtatcc tttcatttgt tgatgcgtta
     2101 aataaacttg aatatataaa acagcgcaac catattggag aaaagattga agcaaagttg
     2161 taggatacac cattgttttt gccggtaaca aaagaagaag ctttaaaact aagctggaac
     2221 aacgttgata tagtcctggt gacgggtgat gcatatgttg atcacccgtc atttggtatt
     2281 gcagtcattg gccgtgtttt agaaaaagca ggcttttcag tgggcattat tgcaatgccc
     2341 aatttcctgg atccatcatc agttgaagtt tttggcaggc caaatctttt ttttggtgta
     2401 acgagcggca atatcgactc aatgctttct cgctttacgg cgtttaaaaa aatacgcaat
     2461 gatgaccctt acatacctgg tggcaaaggt ggcatcaagc catccaatgc agttattacc
     2521 tactgtaatc ttataaaaca gcggtttaag gatgtaccca tagtcattgg cggcattgaa
     2581 gcgtcaatgc gaaggctact ccattatgat tttgttcagg acaagctgcg ccgaagcatt
     2641 atggtggata gccgcgctga tatccttgtg tatggcatgg gtgaatatca gattttggag
     2701 atagcacggc gcctatcagg gggtcagagc ctgcaaggga ttcctggcac agtaactatc
     2761 gccaaacaaa aacctgatga tgctgttgaa ctgcctacag aagaagatgc actggcaaac
     2821 gttgaatcat tgcttgttat gtacaaaatg ttttaccaga attttggcac caaggtgata
     2881 gcacaaaaaa gtggtggcag gtatattatc cacactccac cgcctgctgt aaccaggaaa
     2941 gaacttgatg catattatga actgccgtat gagcgaaaac cccatccaat gtataaagat
     3001 accataccgg cgtttgaaat gatcaagtat tctataaatg cccatcgtgg atgtgcaggt
     3061 ggctgctcgt tttgttcgct taccctgcat cagggaaaga tggtggtcag ccgctcggct
     3121 gattcaataa agcaggagat agttactgtg tcaaaacgca aggtgcatat aagcgatatt
     3181 ggcggaccgt cggcaaacat gtatgggttt acgtgtgcta ttggcggatg taaccgtgaa
     3241 agctgtgtat ttcctgctgt gtgtaagcac ttacggttgg caacagataa atggcttaat
     3301 cttatggaag atgctctgac cctgcccaat gtaaagaagg tttctgttgg ctcaggtata
     3361 cggtatgata tgtttatgca ggggagcaat gctaagaaga atcttgaatt tctggtgaag
     3421 cattttatca gcggtcagct taagattgct ccggaacata cggataaagc ggtgctcaaa
     3481 gccatgcgta a
//
LOCUS       DAOSZP010000195         3485 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_21505_length_3485_cov_3.702041, whole genome shotgun sequence.
ACCESSION   DAOSZP010000195 DAOSZP010000000
VERSION     DAOSZP010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3485
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21505_length_3485_cov_3.702041"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..962)
                     /gene="metG"
                     /locus_tag="PK348_05280"
     CDS             complement(<1..962)
                     /gene="metG"
                     /locus_tag="PK348_05280"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006421951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HXK65657.1"
                     /translation="MKKRFYVTTPIYYVNAEPHIGHAYTTIVADFVNRLYKMRGYETF
                     FLTGTDEHGDKIVKAAMQNNANPQEYTDKISAIFKKQWQSMGLEFNDFIRTTEERHKK
                     VVQYVLQKVYDNGDIYFGSYGGFYCFGCERYFTEKEMVDGKCPDHQKPLEFIKEDNYF
                     FKMSKYQQWLIDYINEHPDFIRPERYKNEVLAMLEGEALEDLCISRPRARLSWGIPLP
                     FDNNFVTYVWFDALINYISAIGYPDSDLFKKFWPVSNHIIAKDIVKPHGVFWPTMLKA
                     AGIEPYQHLNVHGYWNMEAAKMSKSLGNVVTPSQLLEKYGNDEIR"
     gene            complement(966..1829)
                     /gene="ricT"
                     /locus_tag="PK348_05285"
     CDS             complement(966..1829)
                     /gene="ricT"
                     /locus_tag="PK348_05285"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HXK65658.1"
                     /translation="MDITGVKLRCSQQVYYVYTNNLFVRRNSLCVVETEHGIDIGRVF
                     KKLTSLPDESIKVGGKLLRLATQDDLNLLPQIEALEDRAFAVCKEKIIEKKLEMKLVM
                     VKSLFDKTKIIFYFVADGRIDFRELVRDLAAVFRTRIEMRQIGVRDEARMCGGYGTCG
                     RQLCCVNLKEEFEPVSIKMAKDQNMNLHSLKISGMCGRLLCCLGYEYHIYREINESLP
                     SPGFQFIVNNIPFMVEAIDSLKESLILRHDSNTVAIHRDEISNMNGTFVLSDTAIEKL
                     KHSLEVVTEEV"
     gene            complement(1851..2783)
                     /locus_tag="PK348_05290"
     CDS             complement(1851..2783)
                     /locus_tag="PK348_05290"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65659.1"
                     /translation="MTQILGNTVQQSIILHGAKNNILPHSLLFSGLEGIGKKLCAVKL
                     AKTMLCASGNACGACPSCKQIDHATHPDVIIIEPNEKGNIPIGSDEQEGSIRWLIGRV
                     NLASLSQKRVIIINDAHCMNIQSQNALLKTIEEPPAHTFIILISSERNQLLPTIHSRV
                     TEYVFHPLSDSDLVSILQKSGFDGQTCDSIIPVAGGSVSYALTLARNDLYSQLKEAYS
                     AIASFVCQPEVFSYDLSPILQEIGAYRTIDLLINIFRLHCTKNVVPWLHSALDRTHAI
                     EIIKILLELRKGISHNINIRMALKGMLYSLCFTK"
     gene            complement(2788..3231)
                     /locus_tag="PK348_05295"
     CDS             complement(2788..3231)
                     /locus_tag="PK348_05295"
                     /inference="COORDINATES: protein motif:HMM:NF015822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF327 family protein"
                     /protein_id="HXK65660.1"
                     /translation="MIEIVPPNPKKEEQKIVKRKKGTKSSGLHSTFRSTFEGNLEFLL
                     PETLDELLNDLKDQEKKFMDDPSLYNMQRYKALVQRILKILVSEEYVVNTLQRKRRDR
                     ADFVIIQQIQNKLEEITKAYITNNKAFDILKTFDEIRGLILDLTH"
     gene            complement(3233..>3485)
                     /locus_tag="PK348_05300"
     CDS             complement(3233..>3485)
                     /locus_tag="PK348_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HXK65661.1"
                     /translation="DGKFKGTIATDGKVLIGQTGEAITDIKARVVVIGGTVKGNIYAT
                     ERVIMLSTGTIHGNVITPSLIMEDGVIFEGNCTINKRQE"
BASE COUNT          956 a          744 c          708 g         1077 t
ORIGIN      
        1 cggatttcat cgttgccata tttttctaat agctgtgatg gtgttaccac gttgcccaat
       61 gactttgaca tttttgcagc ttccatattc cagtagccat ggacattgag gtgttgatac
      121 ggctcaatgc ctgcagcttt cagcatcgtt ggccagaaaa caccatgtgg tttaacaata
      181 tcctttgcaa taatatgatt ggatacaggc cagaattttt taaacaggtc actatcggga
      241 tatccaatag cgctgatata attaatgagc gcgtcaaacc acacatacgt tacaaagttg
      301 ttatcaaagg gtagcggtat gccccaggaa agtctggccc tggggcgaga aatgcataaa
      361 tcctcaagcg cctcaccttc aagcatagca agtacttcat ttttatagcg ctcgggccgt
      421 ataaagtcgg gatgttcatt aatataatca atcagccatt gctgatattt gctcatctta
      481 aaaaagtaat tgtcttcttt aatgaattcc agaggttttt gatggtcagg gcatttgcca
      541 tcaaccatct ctttttcggt aaaatagcgc tcacagccaa agcagtaaaa accaccgtag
      601 cttccaaagt agatatcgcc gttatcatac actttttgca acacatactg taccactttt
      661 ttatggcgct cttcggtggt gcggataaaa tcgttaaact ccaggcccat tgactgccac
      721 tgtttcttga agatggcact tattttatca gtgtattcct gggggttggc attgttctgc
      781 atggcagctt tgactatctt gtcaccatgt tcatcagtgc cggttaaaaa gaaggtttca
      841 tagccacgca ttttatacag gcgattgaca aaatcagcca caatggtggt ataggcatgg
      901 cctatatgtg gttcagcatt aacgtagtaa atgggcgtgg taacataaaa gcgctttttc
      961 attgattata cctcttcagt aacaacttcc agtgaatgtt ttaatttttc aattgcggta
     1021 tcgctcaaca caaatgttcc attcatattg ctaatttcat ccctgtgaat ggcaacagta
     1081 ttggagtcat gcctcagtat caatgattct tttaagctgt cgattgcttc caccataaat
     1141 gggatgttat tgacgataaa ctgaaaaccc gggcttggta gtgattcatt aatctcacga
     1201 taaatatggt attcatatcc aaggcagcat agtaatcttc cgcacatgcc ggaaattttc
     1261 agagagtgca aattcatatt ctggtctttt gccattttga tggaaaccgg ttcaaattct
     1321 tctttcaggt taacacagca aagctgcctg ccacaggttc cataccctcc gcacatgcgc
     1381 gcttcatcac gcacgccaat ctggcgcatc tcaatgcgtg tcctgaacac cgcagctaaa
     1441 tcccgcacca gttccctgaa gtcaatacgc ccatcagcca caaaatagaa gataatcttg
     1501 gttttatcaa aaaggctttt caccatgaca agtttcatct ctaatttttt ttcaatgatt
     1561 ttttccttgc acaccgcaaa agccctgtct tcaagagcct caatctgagg caataggttt
     1621 aaatcatcct gtgtggcaag gcgtaaaagc ttccccccca cctttattga ttcatcggga
     1681 agggaagtaa gtttcttaaa aaccctgcca atatctatgc catgttccgt ttccacaaca
     1741 cacagggaat tacgacgaac aaaaagattg tttgtatata cataataaac ctgctggctg
     1801 catctgagtt ttacacctgt tatatccata ggaaaacctg cacaaaaaaa ttatttagta
     1861 aaacataaag agtatagcat acctttaagt gccattctaa tattaatatt gtgggaaata
     1921 ccctttcgca gttcaagtaa tattttaata atttctatag catgcgtccg gtcaagtgcg
     1981 ctatgtagcc atggcaccac gttttttgtg cagtgcaatc tgaatatatt aatcaaaagg
     2041 tcaatagttc tgtatgcacc aatttcctgc agtatgggcg atagatcata ggaaaaaact
     2101 tcaggctggc aaacaaagct ggcaatagca ctgtatgctt ctttaagctg tgaatagaga
     2161 tcattacgag caagggtcag agcataggag acagaccctc cagcaacagg gatgatggaa
     2221 tcacatgttt gcccgtcaaa gccacttttt tgcaggatgg atacgaggtc gctatcgctt
     2281 agaggatgaa acacatattc cgtaacacgg gaatgtatgg ttggcagcag ttgattgcgt
     2341 tcagagctaa taaggataat aaacgtatgg gctggtggtt cttcaatggt tttaagcaat
     2401 gcattttgcg actgaatgtt catgcaatga gcatcattga tgatgataac tcgtttttgg
     2461 gaaagggaag ccagattcac gcgaccaata agccatcgta tgcttccttc ctgttcatcg
     2521 ctgccgatgg gaatgttccc tttttcattg ggttcaatga tgataacgtc agggtgtgta
     2581 gcatggtcta tctgcttgca tgaaggacag gccccgcaag cgttgccact ggcacacagc
     2641 atggttttgg caagtttaac tgcacagagt tttttaccaa taccttccag ccctgaaaat
     2701 agcaaagaat gggggaggat gttgtttttg gcaccatgca aaataataga ttgctgaaca
     2761 gtattgccta aaatttgtgt catggtttta gtgggtaagg tccaggatta atcctctgat
     2821 ctcatcaaag gtttttagta tgtcaaaagc cttgttgttt gttatatagg cttttgttat
     2881 ctcttcaagt ttattctgta tttgctgaat aatcacaaaa tcagcacggt cacggcgttt
     2941 tcgttgtaat gtgtttacca catattcttc actcacaagg attttcagta tacgctgaac
     3001 cagggccttg taccgctgca tgttgtacaa tgacggatcg tccataaatt ttttttcctg
     3061 gtctttcagg tcattgagaa gttcatctaa ggtttcaggc aacaaaaatt caagatttcc
     3121 ctcaaaggtt gacctgaatg ttgaatgcag gcctgaggat tttgtgcctt ttttgcgttt
     3181 aacgattttt tgttcttctt tcttggggtt tgggggtact atctcaatca tgtcattcct
     3241 gcctcttgtt tatagtgcag ttaccctcaa aaatgacacc atcttccatg ataaggcttg
     3301 gcgttatgac attgccatga atggtcccag ttgacagcat aataacccgt tcagtggcat
     3361 aaatattgcc tttcacagtg ccgcctatca ccaccacgcg tgcctttatg tcggtaatgg
     3421 cttcgcctgt ctgtccaata agcaccttcc catcagtggc gatagttccc ttgaatttgc
     3481 catct
//
LOCUS       DAOSZP010000196         3481 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_21527_length_3481_cov_3.380327, whole genome shotgun sequence.
ACCESSION   DAOSZP010000196 DAOSZP010000000
VERSION     DAOSZP010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3481
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21527_length_3481_cov_3.380327"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            190..378
                     /locus_tag="PK348_05305"
     CDS             190..378
                     /locus_tag="PK348_05305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65662.1"
                     /translation="MSRIIMMVCSVVVATFIIVGLIDYGYSGCVVGQKSCEDDGYYWV
                     CETCGSETCWIFTGERCK"
     gene            706..1734
                     /gene="recA"
                     /locus_tag="PK348_05310"
     CDS             706..1734
                     /gene="recA"
                     /locus_tag="PK348_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005842655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HXK65663.1"
                     /translation="MSEQKELNQKSQALESAILQIEKQFGKGSIMRLGDEHAKMKVPA
                     ISTGSLELDIALGIGGIPRGRITEIYGPESSGKTTLTLHVIAEAQKAGGIAAFVDAEH
                     ALDPVYAKKLGVNTDELLVSQPDTGEEALEITESLVRSGAIDIVVIDSVAALVPRAEI
                     EGEMGDAHMGLQARLMSQALRKLTGVISKSKTSVIFINQIRHKIGVMFGSPETTTGGN
                     ALKFYASVRLDIRKVDTLKVGEDAVGNRVRVKVVKNKVAPPFRQAEFDIMYDSGISRT
                     AGILDLGVKYNIVQKAGTWYSYKDERIGQGRENAKSFLDQHPDIAKEIEEKIKEQAGL
                     LEKEEAKA"
     gene            1747..2550
                     /gene="folE2"
                     /locus_tag="PK348_05315"
     CDS             1747..2550
                     /gene="folE2"
                     /locus_tag="PK348_05315"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0035998 - 7,8-dihydroneopterin
                     3'-triphosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HXK65664.1"
                     /translation="MPRRSLKDIQSQPDDRNLPINKVGVKDITYPVVVLDRADGLQHT
                     VARINMYVDLPEHFRGTHMSRFVEVLNRYQGGISTKNVRDILEDLKKELNAKTSHVNI
                     EFPYFITKTAPVSGEKSKMDYICTLQGDSSVDYYTLQVKVPLLSVCPCSKEISQYGAH
                     NQRSVVSVSVRAREILWIEELIELVEECGSSQIYALLKREDEKYITERSYENPVFVED
                     IVRNVAVKLLAHPKVLWFLVETESMESIHNHSAYAQIEIDKSKVKRSLA"
     gene            complement(2547..3296)
                     /locus_tag="PK348_05320"
     CDS             complement(2547..3296)
                     /locus_tag="PK348_05320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HXK65665.1"
                     /translation="MTLKNKILIILSLCLLLCVGAYFIISSTFANFEKQLFEKCRIEA
                     LVGARVMSELIEMMIDTRILTKEQVFDRNYIPIPNTNPQKFRTKYDAIFDRYIQKIED
                     EFLKDEDLEFAALVDINGYLPTHNSKYAKPETTDPVYNLKYSRSKRIFNDMVGLNASR
                     FIGPGTLKQLYNRDTGEIMWDISAPVFVKGEHFGAFRIGVSLKRINELKNQMIIIVGM
                     TILVILSITMLMLFLILPRKLYDTDLDIPQY"
BASE COUNT         1090 a          599 c          762 g         1030 t
ORIGIN      
        1 ctctgacccc ttaacagggg aaacattctt tgtggaaaag aaaccattat tgcggaaaaa
       61 acagcatggg atactaacac gaatgataaa aaattgactt ttatactata ttattaatat
      121 tatcgattca ggatgtataa tacaataatg acgtgtgtga agaatatttt taaggaggaa
      181 tgtcgtgtta tgtcacgtat aattatgatg gtatgttcgg tagttgttgc aaccttcatt
      241 attgtaggat tgattgatta tgggtattct ggatgtgtag tggggcaaaa atcatgtgaa
      301 gatgatgggt actattgggt gtgtgaaacg tgtggttctg aaacatgctg gatttttaca
      361 ggggaacgat gtaaataata catttttcat tcattttttt aatcatatat tgcatcacat
      421 caataattat ttgttaactc atttgtatga aatgagaaga agttttattc attctctata
      481 atataaaaaa acaggggaac aacttaaagg tttatataca gtggtcgaag agaaaaaata
      541 catataagtt gacatttttt acactctatg tgtaatatga cataatgcta ttttacagta
      601 actatcgcac acaaaaattt tttgatgttt ttttgatatt acttgttatt tttttgcaga
      661 attagcgtta tatattggta taaaccacac aatgaggtaa taatcatgag cgaacagaaa
      721 gaactcaatc aaaaatcaca ggcgctggaa tccgctattt tgcagataga aaagcagttt
      781 gggaagggct ccatcatgag gcttggcgat gagcatgcaa agatgaaggt gccagctatt
      841 tcaacaggct cactggagct tgacattgcc ctgggtattg gcggtatacc gcgagggcgc
      901 atcaccgaaa tttatggtcc tgagtcatcc ggcaaaacca cgcttacact gcatgtcatt
      961 gccgaggcac agaaggcagg tggtattgct gcatttgttg atgctgagca tgctcttgat
     1021 ccggtatatg caaaaaaatt aggcgtcaat accgacgagc ttttagtgtc gcagccggat
     1081 accggtgagg aagcgcttga aatcacggaa tcgttggtgc gctcaggtgc catagatatt
     1141 gttgtcattg actcggttgc tgcgctggtc ccgcgtgccg aaattgaagg cgagatgggt
     1201 gatgctcaca tgggattgca ggcacgtctt atgagccagg cgctgcgaaa gcttaccggt
     1261 gtcatatcaa aatccaaaac aagcgtcata tttattaacc agattcgtca taagattggc
     1321 gttatgtttg gctcgccaga gacaaccacc ggtggcaatg cgctgaagtt ttatgcttca
     1381 gttcggcttg atatacgcaa ggttgatacc ttaaaagtag gagaggatgc tgttggtaac
     1441 cgtgtacgtg ttaaggttgt taaaaataag gttgctccac cattcagaca ggctgaattt
     1501 gatatcatgt acgatagcgg catatcacgc actgcaggca ttttagatct gggtgtaaaa
     1561 tataatattg tacagaaagc aggcacctgg tattcataca aagacgaacg cattggtcag
     1621 ggacgtgaaa atgcaaaaag ctttttagat cagcatcctg atattgctaa ggaaatagaa
     1681 gaaaaaatta aagagcaggc aggtttgctt gaaaaagaag aagcaaaagc gtaacgaagt
     1741 accataatgc cgcgaagaag tttaaaagat atacaatcac agccggatga cagaaattta
     1801 ccaataaaca aagttggtgt taaggacatt acctatccgg ttgtggtgct tgaccgtgct
     1861 gatggattgc aacataccgt tgctcgcatc aacatgtatg tggatttgcc cgaacatttc
     1921 cgtggcacac atatgtcccg ttttgttgag gtgctcaacc ggtatcaggg tggcataagc
     1981 acaaaaaatg ttcgtgacat cctggaagat ttaaagaaag aactcaacgc aaagacatcc
     2041 catgtaaata ttgaattccc ctatttcata accaagactg ccccagtcag tggtgaaaaa
     2101 tctaagatgg attatatatg tacgcttcag ggcgattcat cagttgatta ttacacattg
     2161 caggtaaagg taccacttct gtcagtgtgc ccatgttcaa aagagattag ccagtatggg
     2221 gcgcacaatc aacgaagtgt tgtcagtgtt tcggtacgtg ccagggagat actgtggatt
     2281 gaagagctta ttgaactggt tgaagaatgt ggttcaagcc agatatatgc gcttcttaag
     2341 cgtgaagatg aaaaatatat tactgaacgg tcatatgaaa atcccgtgtt tgttgaagat
     2401 attgtgcgca atgtagctgt gaaattgctg gcacatccaa aagtgctgtg gtttctggtt
     2461 gaaacagaaa gtatggagtc aatacacaat cactccgcgt atgcacagat agaaattgat
     2521 aaatcaaaag tgaaacgaag cctggcttaa tactgcggta tatccagatc agtatcgtag
     2581 agttttcgcg gtaaaattaa aaatagcatg agcatggtta tacttaaaat gacaagtata
     2641 gtcataccca caataataat catctgattt ttcaattcat taatgcgctt caatgaaaca
     2701 ccaatcctga aagcgccaaa gtgttcacct ttaacaaaca ccggtgctga aatgtcccac
     2761 attatttcac cggtatcacg gttatacagc tgttttaatg ttcccgggcc aataaaccgt
     2821 gatgcattga gtcctaccat gtcattaaat attcgtttgg aacggctgta tttgagattg
     2881 taaacagggt cagttgtttc tggtttagca tatttgctat tgtgagtggg cagatacccg
     2941 tttatatcaa cgagtgctgc aaattccaga tcttcatctt tcaaaaattc gtcttcaatt
     3001 ttctgaatat agcggtcaaa tatggcgtca tattttgttc tgaacttctg cggatttgta
     3061 tttggtatgg gaatataatt tcggtcaaaa acctgttctt tagtaagaat gcgtgtgtca
     3121 atcatcatct caatcagttc actcataacg cgtgcgccaa ccagtgcctc aatgcggcac
     3181 ttttcaaaaa gctgcttttc aaagttggca aatgtgcttg atatgatgaa atacgcacca
     3241 acgcacagta acaggcaaag acttaaaata attaagattt tatttttcaa tgtcatggga
     3301 cagcctcagg attatcaaaa ttgtaagcat accagcaatg aagtatacat gcaaaatcac
     3361 atcgttgtca attattttat agtgttatta ttattttata aaattttttt aaaaatataa
     3421 gtgggcgata gttactgctg aaaaactcat tgctgtgata taataatcgc tggtttggaa
     3481 t
//
LOCUS       DAOSZP010000197         3456 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_21697_length_3456_cov_3.280800, whole genome shotgun sequence.
ACCESSION   DAOSZP010000197 DAOSZP010000000
VERSION     DAOSZP010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3456
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21697_length_3456_cov_3.280800"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(54..821)
                     /locus_tag="PK348_05325"
     CDS             complement(54..821)
                     /locus_tag="PK348_05325"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010038738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HXK65666.1"
                     /translation="MEYNAMELMICVAARNLEDGATVVVGTGAPCAAAMLAQKLYSPN
                     LCIMFEAGGVAPILPTMPISVGDSRTFHKAIMASSMPEVMETCQRGMVDYTFLGGAQI
                     DMYGNINSTMIGDDYEKPKVRFPGSGGDNDLASLCWRTMMMTVQDARRFTDKLDFITS
                     PGYLQGGRSRYEAGLPEGTGPYRIITNMAVMGFDEETKWMTVLSINPGYSRKDVQDNC
                     GFELKWAKKIEDTKPPTDEELRILREEVDPHRYIIGR"
     gene            complement(831..1847)
                     /locus_tag="PK348_05330"
     CDS             complement(831..1847)
                     /locus_tag="PK348_05330"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014957258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HXK65667.1"
                     /translation="MAMSVHDSDMKETMVSGKNELFTDPDPDHARAFFRNKSRSMKPK
                     LSNVKDVVRDMIKDGDYIAIGGFGANRIPTAIIHEIVRQRKKHLGFAGHTSTHDFQVL
                     VAGECIDRCDVAYIIGLEARGLSPNARRAVESGKIKLTEWTNASLSWRLKAAAMGISF
                     IPTRNILGTDTFAQSAAKVITCPFTGKKYAAMPALFPDFAAIHVHEADIYGNCRVYGA
                     SVSDFDLAKASKRVVITAERIIPHDEIRREPEKTVIPFWCVDAVIEVPYGSYPGNMPY
                     EYFSDEEHLKTWLRVEKDPKELEKFLQEQIYGVDNFYDYLNLNGGIEKMKKLRAIEYL
                     INNK"
     gene            complement(1922..>3456)
                     /locus_tag="PK348_05335"
     CDS             complement(1922..>3456)
                     /locus_tag="PK348_05335"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HXK65668.1"
                     /translation="YIVSHSESKFMVVENNLQLQKGLYTAKRYPGLQKIIVIDMNGVG
                     ANPLVMSFAELLEKGKKLYDANPGKFKELADAVTPDDLATIVYTSGTTGVPKGAMITH
                     KNIMAVVMALHKIEPHYAYDTDQTVPFLPLSHVFERIAGHFYGMYVGITASYAESLDT
                     ILQDIQEKRPTVVLAVPRVLEKVYQRIISQVQEQSPFKQKVFYWGQKVGNKISTLREQ
                     KKKPGLLLKLKYKIAYAIIFKKLQNALGGRVRWMTASGAPTAREIILFFNSAGIMVIE
                     GYGMTECCAPATMSNIADYRIGTVGRPLPGVDIKIADDGEILIKGDNVFKGYWKMPEE
                     TKESFTPDGYFMSGDIGVFDDAGFLMITDRKKDLIITSGGKNVAPQKIENLIKSDPLF
                     TQAIVIGDKRKYLTALVNISQEQAEHVAREKGISFTSFNDLLENEQFQKVVEEHIEKC
                     NQKLARYETVKKVGIIRNEFSKETGELTATLKVKRKIVQEKYKSIIDSLYEEDAKTVQ
                     VA"
BASE COUNT          947 a          833 c          573 g         1103 t
ORIGIN      
        1 ttgaacagat cttcgttcca ccgcaactat tttgactttt ttatcattcc tccttatctt
       61 ccaatgatat accggtgagg atcaacctct tcacgtaaaa ttctgagttc ttcatcagtt
      121 ggcggtttgg tgtcctcaat cttctttgcc cattttaact caaatccaca attgtcctgc
      181 acatctttac ggctgtatcc tggatttatt gaaagcactg tcatccactt ggtttcctca
      241 tcaaaaccca tcactgccat attggtgata atccggtatg gacctgtccc ttcaggcaaa
      301 ccggcttcat agcggcttct tcctccctgt agataacccg gagaagtgat aaaatccaat
      361 ttatcagtaa acctgcgagc atcctgcact gtcatcatca tcgtgcgcca gcacagtgag
      421 gctaaatcat tatcaccacc ggaaccagga aagcgtacct taggtttttc ataatcatcg
      481 ccaatcattg tagaatttat attgccatac atatctatct gtgcaccgcc caaaaaggta
      541 tagtcaacca taccacgctg gcaggtttcc atgacctctg gcattgagct tgccataatt
      601 gctttatgaa atgtacggga atctccaacc gaaatgggca tggtgggtaa tatgggtgca
      661 acgccaccag cctcaaacat gatacataaa tttggtgaat atagcttttg agctaacatt
      721 gctgctgcac agggcgcacc tgttcctaca acaactgtcg ccccatcctc taaattgcgt
      781 gctgcgacac atatcatgag ttccattgca ttatattcca tggccccctc ctacttattg
      841 ttaatcaaat attcaatggc tctgagcttt ttcatctttt ctatcccacc atttaagttg
      901 agatagtcat aaaaattatc caccccataa atctgttcct gtaaaaactt ctctaactct
      961 ttggggtcct tttccacacg tagccatgtc ttcaggtgct cttcatcact gaagtattca
     1021 taaggcatat tgcctggata gctgccatac ggaacctcaa tcactgcatc cacacaccag
     1081 aatggaataa ctgttttttc aggctcgcgg cgtatttcat catgtgggat aatacgctca
     1141 gcagttataa ccactcgttt ggaagctttt gctaaatcaa agtcagatac cgatgcacca
     1201 tacaccctgc aattcccata gatatccgct tcatgaacat gaatggctgc aaagtcaggg
     1261 aacaatgctg gcattgcagc atactttttg ccggtaaatg ggcaggttat cactttggca
     1321 gcactctgcg caaacgtatc ggtacccaga atattgcgtg ttggaataaa agaaattccc
     1381 attgctgcag ctttcaatcg ccatgaaagt gaggcattgg tccactcagt aagtttgatt
     1441 ttaccgcttt ccacagcacg gcgtgcattg ggcgataggc ctcgcgcttc caaccctata
     1501 atgtaagcca catcacagcg atcaatgcat tcacctgcca ccagcacctg aaaatcatgg
     1561 gttgatgtgt gcccggcaaa gcctaaatgt ttttttcgct gacgtactat ttcatgaatg
     1621 atagcagttg ggatacggtt tgcaccaaaa ccgccgatgg ctatgtaatc accatctttg
     1681 atcatatccc tcaccacatc ctttacattt gatagcttag gtttcatgga acgtgattta
     1741 ttcctgaaaa aagctcgtgc atgatctgga tccggatcag tgaataattc atttttaccg
     1801 gataccattg tttctttcat atcactatca tgtacactca ttgccatagt ttcctcctca
     1861 gcctaataaa ttatacatac attataatat tggttgaaca tacaatcaaa tatattcgtt
     1921 tttaagctac ctgtacagtt ttggcatcct cctcatacag agaatcaatg atggatttat
     1981 acttttcctg cacaatttta cgcttaactt tgagtgttgc agttaattca ccggtttctt
     2041 ttgaaaattc atttcgtata atacccacct ttttcactgt ttcataccgt gccagctttt
     2101 gattgcattt ctcaatatgc tcttccacaa ccttctggaa ctgttcattt tccagtaaat
     2161 cattaaaact tgtaaatgaa ataccttttt ctcttgcaac atgttcagcc tgttcctgac
     2221 tgatattaac cagtgctgtt aaatattttc gcttatcacc aataactatc gcctgtgtaa
     2281 acaatgggtc tgattttata agattttcaa tcttctgcgg tgctacgttt ttaccaccag
     2341 atgtaataat cagatctttc ttcctgtcag taatcatcaa aaatcctgca tcatcaaata
     2401 ctccaatatc accactcata aaatatccat caggtgtaaa agattctttt gtttcttcgg
     2461 gcatcttcca gtaccctttg aacacattat cgcctttaat aagtatctca ccatcatcag
     2521 ctattttaat atcaacacca ggcaaaggcc ttcccactgt accaatgcgg taatcagcaa
     2581 tattactcat agtagccggt gcacaacatt cagtcattcc atagccttct atgaccatga
     2641 tacctgctga attaaagaat aatattattt cacgtgcggt tggagcgcct gatgctgtca
     2701 tccagcgaac tctaccaccc aatgcattct gcaatttttt aaatatgatg gcatacgcta
     2761 ttttatactt taatttcagc aacaaccccg gcttcttctt ctgttcccgc aaggtgctta
     2821 ttttattacc aactttctgc ccccagtaaa ataccttctg tttaaacggg gactgttcct
     2881 gaacctgcga aataatacgc tggtaaactt tttcaagcac acggggcact gcaagcacaa
     2941 ccgttggccg tttttcctga atgtcctgta agatagtatc taaactttct gcatatgaag
     3001 cagttatgcc aacatacata ccatagaaat gtccggctat tcgttcaaat acatgactca
     3061 atggcaaaaa tggaactgtt tgatcggtat catacgcata atgtggctca atcttgtgca
     3121 atgccatgac tactgccata atgtttttat gggtaatcat tgcacctttt ggcacaccag
     3181 ttgttcctga tgtatatact atagttgcaa gatcatccgg cgttactgca tcagctaact
     3241 ctttaaactt accaggatta gcatcataca attttttacc tttttcaagt aattcagcaa
     3301 agctcattac cagtggattt gcaccgacgc cattcatatc aattactata atcttttgta
     3361 atcctggata ccgttttgca gtgtataatc ccttttgtaa ttgtagattg ttttccacaa
     3421 ccataaactt tgattcagaa tgggaaacaa tatact
//
LOCUS       DAOSZP010000198         3454 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_21709_length_3454_cov_2.982642, whole genome shotgun sequence.
ACCESSION   DAOSZP010000198 DAOSZP010000000
VERSION     DAOSZP010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3454
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21709_length_3454_cov_2.982642"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1154
                     /gene="uvrC"
                     /locus_tag="PK348_05340"
     CDS             <1..1154
                     /gene="uvrC"
                     /locus_tag="PK348_05340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HXK65669.1"
                     /translation="YSQAMRFESAAKVRDMIFDIQKMLQAQHVVTPLGQNQDYIGITT
                     HKDEAYILVFQFRHGSLTGKRIFVYNNAEYTSRSSLIAQFIVEYYATNEPPQTIVIPD
                     MIEDRTIIEEYLSSKTSQPVHAVTPRTDHEKAVMQMLHKNLDLAIAQKIAPGWQKALF
                     ELQEVLQLPALPMDIECFDISNIQGNFAVASMVHFTEGMPNKKEYRRFRIRAYTKPDD
                     PGMIHEVVGRRLQHLLNEGLPLPDLLVIDGGKGQLSRAREAMETLECSVPIIALAKRL
                     EEIYLPQSSDPLQLPKTSPALKLLQAIRDEAHRFAITYHRNLRDKTMTASVLDRISGV
                     GPQYKKKLLQSFDTIEHIKQSDAQTIAQIAKIPATVAQKIYDFFHKGES"
     gene            complement(1173..1820)
                     /locus_tag="PK348_05345"
     CDS             complement(1173..1820)
                     /locus_tag="PK348_05345"
                     /inference="COORDINATES: protein motif:HMM:NF014025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HXK65670.1"
                     /translation="MLLCNAMPESTFIPAVFFTLFFVVDPLGLIPVFVAYLAPYDAAY
                     RKKVIIKSTTISIIVSLFFIFSAHIFLRFIGITPGAFMIAGGILLFVIAMDMLLARPR
                     RTQKLNDKEYEADTDIAVFPLAIPFLCGPGNIAALLMFSSQAQGDIVKLAIISVISAV
                     TFLIAMVVMALAGRLQQVLGQTGISVIHKLMGLILSAMAVQFIINGLVQSNIIGR"
     gene            complement(1807..3393)
                     /gene="serA"
                     /locus_tag="PK348_05350"
     CDS             complement(1807..3393)
                     /gene="serA"
                     /locus_tag="PK348_05350"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: protein motif:HMM:TIGR01327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HXK65671.1"
                     /translation="MKKKILITDDIAPEGLQLLQSNPDFDIVYKPGLDGDMLKQHLAT
                     AHALIIRSATHVTADIINEAKVLEIIARAGIGVDNVDVEAATAKGIIVTNAPGGNTIS
                     TAEFALSLICSLARNIPQADASMKKQLWEKTKFEGVELRRKVIGIIGLGRVGSELAKL
                     CKALGMQVVGFDPYLAKEKLAAMDIDIADLETIWKKADFISVHTPLTDETKNLINADV
                     IKKLKPTVRIVNCARGGIVNEQDLYKALKEKRIAGAALDVFTQEPPENLPPFHELDNV
                     ILTPHLGAYTYEAQVSVAIEAAQTVIDYFTKGVIHDSINYPSVDMQQYDFLKPFINLA
                     ERMGKFQAIMLGGKVRSVQLYYSGEFGKYSREPLTAAYLKGLLSPYIDRPINYVNATM
                     IAAERGIKVHVGESTQSGDWAHLVQVKAEGGTLTNELWGTVIAKQPWVVRCDEYLIDF
                     IPSGKMLVMHNNDVPNVIGAIGMFLGKNHINIANLHLARTRRGGKALVILEIDEDIPA
                     DVLEKLAELPEILDVKYVVV"
BASE COUNT         1029 a          784 c          656 g          985 t
ORIGIN      
        1 catattcaca ggcaatgcgt tttgaaagtg cagccaaagt gcgcgacatg atatttgaca
       61 ttcagaagat gctgcaagca cagcacgtcg tcacaccatt ggggcaaaat caagattata
      121 ttggcattac tacacataag gatgaagcgt atattctggt ttttcaattc aggcatggta
      181 gcctgacggg taaacgtatc tttgtgtata ataatgccga gtacaccagc cgctcttcac
      241 ttattgccca atttattgtt gaatactatg ctaccaatga accaccacaa accatcgtca
      301 ttcctgacat gattgaagat aggacaataa ttgaagaata tttaagctct aaaacctcac
      361 agcctgtaca tgccgttact cccagaacag accatgaaaa agccgttatg cagatgcttc
      421 acaaaaattt agatcttgcg atagctcaaa agattgctcc aggctggcaa aaggctttat
      481 ttgaattaca ggaagtgttg caacttccag cactcccaat ggatatagaa tgctttgata
      541 tatcaaacat tcagggaaat tttgctgttg cctctatggt gcattttact gaagggatgc
      601 caaacaaaaa ggaatatcgc cgcttccgca tacgcgccta tactaagcca gacgatcctg
      661 gcatgattca cgaggttgtg ggaaggcgac tgcaacacct tttaaatgaa gggcttccac
      721 tgccggacct ccttgttatt gatggcggta aaggacagct ttctcgtgca cgcgaggcaa
      781 tggaaacgtt agaatgctct gtaccaatta tagcacttgc aaaacgatta gaagaaatct
      841 acctgcctca aagctctgac cccctgcaac ttcctaagac atcacctgca ttgaaactgc
      901 tgcaggctat ccgtgatgaa gctcatcgct ttgctataac ttatcaccgc aaccttcgtg
      961 ataaaaccat gactgcatct gtccttgatc gtataagcgg tgttggtcct caatataaaa
     1021 agaagctgtt gcaaagcttt gacacaatag aacacatcaa acaatcagat gcacaaacta
     1081 ttgcacaaat agcaaagatt cctgcaacag ttgctcaaaa aatctatgat ttttttcaca
     1141 agggggaaag ttaaaaagct tttttacagt aactatcgcc caattatatt actttgtact
     1201 aacccgttaa taataaactg aactgccata gccgaaagga taagccccat caatttatga
     1261 atgactgaaa tccccgtttg ccccagaacc tgctgaagcc tgcctgcaag tgccataaca
     1321 accatggcaa tgaggaatgt aaccgctgag ataactgata ttatggcaag ctttacaata
     1381 tcaccctgcg cctgcgaact gaacataagc agtgctgcaa tatttcccgg accacacaaa
     1441 aaggggatag ccagcgggaa cacggcaata tcagtgtcag cttcatattc tttatcgtta
     1501 agcttttgcg tccgccgtgg ccgtgcaagt aacatgtcca tagcaatgac aaataacagt
     1561 attcctcctg caatcataaa tgcgccaggc gttatgccta taaagcgcaa aaaaatgtgg
     1621 gcactgaaaa tgaaaaatag tgaaacaatt attgagatgg ttgtggattt gatgattacc
     1681 tttttacggt acgcagcgtc atacggtgct aagtatgcca caaatacagg gataaggccc
     1741 aatgggtcca ccacaaagaa aagcgtaaag aagaccgctg gtataaatgt tgactcaggc
     1801 atagcgttac acaacaacat actttacatc taaaatctca ggcagctctg caagtttttc
     1861 aagcacatct gcagggatat cctcatctat ttccagtatt accagtgctt tgccgccacg
     1921 gcgtgtccgt gccagatgca ggttggcaat attaatatga tttttaccta agaacatgcc
     1981 tattgcacca atgacattgg gaacatcatt attgtgcata accagcattt ttcctgaagg
     2041 tataaaatca ataagatatt catcacagcg caccacccac ggctgctttg caatgaccgt
     2101 cccccataat tcattggtaa gcgtgccacc ttcagcctta acctgcacaa ggtgtgccca
     2161 atcacctgat tgtgtgcttt caccaacatg tactttaatg ccacgttctg ctgctatcat
     2221 ggtagcattg acatagttta tgggcctgtc aatatagggc gatagtaacc ctttaaggta
     2281 tgctgctgtt aacggttccc tgctgtattt accaaactct cctgaataat acagttgcac
     2341 agaacgcacc ttgccaccta acatgattgc ctgaaattta cccatacgct ctgcaagatt
     2401 aataaatggc ttgaggaaat catactgctg catatcaaca gatggatagt tgatactgtc
     2461 atggataaca cccttggtaa agtaatcgat tactgtttgt gctgcctcaa ttgctaccga
     2521 aacctgtgct tcatacgtat atgcacctaa atgaggcgtg aggataacat tatcaagttc
     2581 atggaatggc ggcagatttt caggtggttc ctgagtgaat acatctaatg ctgcacctgc
     2641 tatccgtttt tctttcaatg ctttgtataa gtcctgttca ttaacaatgc caccacgcgc
     2701 acagtttaca atccgcactg ttggttttaa tttttttata acatcagcat taatcaagtt
     2761 ttttgtttca tcggttaatg gtgtatgaac gctgataaag tctgcttttt tccatattgt
     2821 ttctaaatcg gcaatgtcta tatccatagc tgctaatttt tcttttgcaa gataaggatc
     2881 aaatcccact acctgcatcc ccaaagcctt gcataattta gctagctctg accccacccg
     2941 tcctaagcca attatgccta taactttacg cctcagttca accccttcaa attttgtctt
     3001 ttcccataat tgctttttca ttgatgcatc cgcctgtggt atattgcgag caagcgaaca
     3061 tataagtgac agagcaaact cggctgttga aatggtattt ccacctggag cattggttac
     3121 aatgattcct tttgctgttg cagcttcaac atccacatta tcaacaccaa tgccagcacg
     3181 ggcaataatt tccaatactt tagcttcatt aatgatatca gcggtaacat gggttgctga
     3241 tcggataatg agggcatgag cagtagccag gtgttgtttc aacatatcac catcaagccc
     3301 tggcttgtat actatatcaa aatcaggatt gctttgtaaa agctgcaacc cttcgggagc
     3361 aatgtcatca gtgataagaa ttttcttttt catggttttc tccctccata aattattgaa
     3421 tcttataata tagtaattgc tattaaatgt caat
//
LOCUS       DAOSZP010000199         3445 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_21781_length_3445_cov_3.264602, whole genome shotgun sequence.
ACCESSION   DAOSZP010000199 DAOSZP010000000
VERSION     DAOSZP010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3445
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21781_length_3445_cov_3.264602"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..372)
                     /locus_tag="PK348_05355"
     CDS             complement(<1..372)
                     /locus_tag="PK348_05355"
                     /inference="COORDINATES: protein motif:HMM:NF014409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65672.1"
                     /translation="MKNTYTITAIIQARMQSTRLPGKSVLPLAGKPLLYHVIERVKAI
                     QQVTQVVVAIPNDDDNNPLADIAELCNVNVFRGSLHNVLERYFFAYKQYGGNYIVRIT
                     ADNPFTDSHFASVAIEEELRTN"
     gene            complement(389..1888)
                     /locus_tag="PK348_05360"
     CDS             complement(389..1888)
                     /locus_tag="PK348_05360"
                     /inference="COORDINATES: protein motif:HMM:TIGR04321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spiro-SPASM protein"
                     /protein_id="HXK65673.1"
                     /translation="MNTQALIFVNSSINNDNPLTFNGQFIPDSIAQKIQSLPDIESIH
                     YCIPESYTGKLKQAASVFIYSNTDTPEMWQQIFTQTGALHIIKIPADAAFFQSSILSE
                     MLDTHIKYLSEFTFSYNLPSGYACEIISKSVLENPLANPTVQNSLCDIVKNNLNHFDV
                     ELYYKEPDLRWHRVNFRAANIRDRLVMQNIIAINNQTPAYELLQDIIDNHPEVLFTTP
                     SYVEIELCGHCNLDCIFCYRNILSPQHGTMELSTLQTLLEKMRIFNTPYAVCFGGSGE
                     PLQHPQFYKALELCNNEDLIHTIVIETNGIHADAGYASALASFTGNKVTTIVNINGHN
                     PETYRKLHNADYFNTVHNNILKLKEFLETNSKLNNNLYIQILKINETESFLDSYYDFW
                     EQHNIPIILQKQNTYIGRIPDKRYSDLTPLQRKPCWHLRQDLYILSDGTIAFCKQDID
                     GAAKRGNIITDDLEAMWKQQREHFVNDYKQNRAKKPNCSVCDEWYTYNL"
     gene            complement(1897..2838)
                     /locus_tag="PK348_05365"
     CDS             complement(1897..2838)
                     /locus_tag="PK348_05365"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HXK65674.1"
                     /translation="MKKKLCIIIVTIVTAVPVFAWETFDRVIAVVNDTAIIQSELMRR
                     YQIVTKKQTPKKPEELNSILDNLIERALIEQEAREQAIIISDEKVMTHIHNIMKSQKI
                     PSLDVFKKMVEEKEGISFEEYKEEIRQSLLTEMLVSLAIGVSPPSRDEAYQWYKENKN
                     KLGNEVHIKQIVIRPKNSSFAEEKRANELINQIRDRILKGESFEKLASQYSEDPSAKN
                     GGDIGWIALAEMDPYMANTVFRMKKPGEISTVIKTQTGYTIVKLIDSRPTSFETVQDR
                     ILNMLYQRNMGEQFKKWIVTRRAQSDIKIYLENYRQS"
     gene            complement(2843..>3445)
                     /gene="murI"
                     /locus_tag="PK348_05370"
     CDS             complement(2843..>3445)
                     /gene="murI"
                     /locus_tag="PK348_05370"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HXK65675.1"
                     /translation="NVKMIVIACNTSSAAALPTLQQENTIPIIGVINAGARAACKRVH
                     NNVIGVIGTRATVSSKSYEKAIHAINPAITVIQQHATMLVSLIEEGWIEDDVTRLTIQ
                     RYLQHMIDQGVKTLILGCTHFPILKNTIHEVYPQLDLVDTGEEIAREVNDILTKKNLA
                     NNSSSGTIELYASDITDTMQNLKKLFFNGHDVPIEKLVIG"
BASE COUNT          899 a          642 c          716 g         1188 t
ORIGIN      
        1 gtttgtgcgt agttcttctt caatagcaac cgatgcgaaa tggctgtcgg tgaaaggatt
       61 gtcggcagtg atgcgcacaa tatagttacc accatactgc ttatacgcaa aaaagtagcg
      121 ttcaagcaca ttgtgtaaag aaccgcggaa tacgttaaca ttgcaaagtt cagcaatatc
      181 agctaatggg ttgttgtcat catcattggg gatggcaact accacctgtg tgacctgttg
      241 tattgctttt acacgctcaa taacatggta tagcaggggt ttgcctgcaa gtggtaaaac
      301 cgatttacct ggcagccttg ttgattgcat gcgtgcctga ataattgctg tgatggtgta
      361 agtatttttc attgtgcggt actacatgtt acagattata tgtataccat tcatcgcata
      421 cagaacagtt aggttttttt gcacggtttt gtttatagtc attaacaaaa tgctcacgtt
      481 gctgtttcca catagcttcc aggtcatcag ttatgatgtt gccgcgtttt gctgctccat
      541 ctatatcctg tttgcagaat gcaatcgtgc cgtcgcttaa aatatataaa tcctgtcgca
      601 ggtgccagca tggttttcgc tgcagtggtg ttaagtctga atagcgttta tcaggaatac
      661 gtcctatgta tgtattttgc ttttgcagta tgatgggtat gttgtgctgt tcccagaaat
      721 cgtagtaact atctaaaaaa ctttcagttt catttatttt cagtatttga atataaagat
      781 tattgttgag tttgctgttt gtctcaagaa attctttcag tttgagtata ttgttatgaa
      841 cagtgttaaa ataatcagca ttgtgcagct ttctgtaggt ttcgggatta tggccattaa
      901 tattgactat ggtggttact ttattacctg taaaagaagc cagtgctgat gcatatccgg
      961 catcggcatg tatgccgttt gtttcaataa caatggtatg tatcaaatct tcgttgttgc
     1021 aaagttccag tgccttatag aactggggat gctgtagtgg ctcaccactt cctccaaaac
     1081 atactgcata aggggtgttg aatattcgca tcttttcaag aagcgtttgc agtgtggata
     1141 gttccatagt gccatgctgg ggtgatagta tattccgata gcagaatatg caatcaaggt
     1201 tacagtgccc acacagttca atttctacat aggaaggtgt ggtaaaaaga acttctgggt
     1261 gattgtcaat gatatcctgc aacaattcgt atgcaggtgt ctggttattg atggcaataa
     1321 tattctgcat gacaagcctg tcgcggatgt tagcagccct gaagtttact ctgtgccagc
     1381 gcaaatcggg ttctttatag tacagttcca catcaaaatg gttaaggttg ttttttacaa
     1441 tgtcgcacag tgagttttgt accgttgggt ttgcaagcgg attttcaaga actgattttg
     1501 atataatctc acatgcatac cctgaaggca ggttgtaaga aaacgtaaat tctgaaagat
     1561 atttaatgtg agtatcaagc atttcagata gtattgaaga ctgaaagaac gctgcatcag
     1621 caggtatctt tatgatgtgg agtgcacctg tctgtgtaaa tatctgctgc cacatttcag
     1681 gggtgtcagt gtttgaataa ataaacactg aagcagcctg cttgagtttt cccgtgtatg
     1741 actcagggat acagtaatgg atggattcaa tgtcaggcag tgattgtatt ttctgtgcaa
     1801 tactatcagg tataaactgc ccgttgaatg ttaaaggatt gtcattgtta attgagctat
     1861 tgacaaaaat taaggcttgc gtgttcacgg tatttcctag ctctgcctgt aattttcaag
     1921 atatatttta atatcggatt gcgccctgcg ggttacaatc cattttttaa actgttcgcc
     1981 catattgcgc tgatatagca tattcaaaat tctgtcctga acagtctcaa atgatgttgg
     2041 tcgggaatct attagtttta cgattgtata gcctgtttgc gtttttataa cagttgatat
     2101 ttcaccaggt tttttcatcc tgaatacagt gtttgccata tacggatcca tctcagctaa
     2161 ggcaatccag ccaatatcac cgccattctt tgcacttgga tcttcagaat attgtgaagc
     2221 aagtttttca aatgattcgc cttttaatat acggtcgcgt atctgattta tcagttcatt
     2281 ggcacgcttt tcctcggcaa aacttgaatt ttttggccgt atgactatct gcttgatatg
     2341 cacttcattc cccagtttat ttttattttc tttataccac tggtacgctt catctcgtga
     2401 tggcggtgaa actcctattg ccagtgacac cagcatttca gtaagcaggc tctggcgaat
     2461 ttcttcttta tattcctcaa aactgattcc ttctttttcc tcaaccatct ttttaaaaac
     2521 atccagagaa ggaatcttct gcgatttcat aatgttatgt atgtgtgtca ttactttttc
     2581 atcgctaatg attattgcct gctcacgggc ttcctgctca atgagtgctc tttcaatcag
     2641 gttatcaagg atggaattta gctcttcagg ttttttgggt gtttgtttct ttgtaactat
     2701 ctgataacga cgcataagct cgctctggat tattgccgta tcattaacaa cggcaattac
     2761 tctgtcaaaa gtttcccagg caaaaacagg tacggctgta acgatggtta caattataat
     2821 gcatagtttt tttttcattg agctatccta taacgagttt ttcaatagga acatcatgcc
     2881 cattgaagaa taattttttt aaattttgca tggtatcagt aatgtccgat gcatagagct
     2941 ctattgtgcc tgatgatgaa ttatttgcaa gatttttttt agttaaaatg tcatttactt
     3001 ctctggcgat ttcttcccct gtatcaacta aatctagctg gggatacact tcatgaatgg
     3061 tgtttttaag tattgggaaa tgggtacagc caagaatgag ggtttttaca ccctggtcaa
     3121 tcatatgctg cagataccgt tgtatggtta acctggtaac atcatcttca atccaaccct
     3181 cctcaataag ggataccagc atggttgcat gttgctgaat tactgttata gcgggattta
     3241 ttgcatgaat tgccttttca tacgatttgc tgcttacagt tgcacgggtt ccaataacac
     3301 caatcacatt gttgtgcacg cgtttacatg ctgctcgggc acctgcatta ataacgccaa
     3361 taataggaat agtattctcc tgctgcagag tagggagggc tgctgctgat gatgtgttgc
     3421 atgctatcac aatcatttta acatt
//
LOCUS       DAOSZP010000200         3442 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_21812_length_3442_cov_3.208739, whole genome shotgun sequence.
ACCESSION   DAOSZP010000200 DAOSZP010000000
VERSION     DAOSZP010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3442
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21812_length_3442_cov_3.208739"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..316)
                     /locus_tag="PK348_05375"
     CDS             complement(<1..316)
                     /locus_tag="PK348_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006425038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65676.1"
                     /translation="MRLAEPTIDHTPGKIPTDLEHLRKLFIMPDSPDKFMQFGYELLD
                     LIHSFFKEKGGIHSEISLPELACLYNNIDIPRDPKLLKDILLEIKQKVIKHSVKVGNP
                     YYI"
     gene            790..1521
                     /locus_tag="PK348_05380"
     CDS             790..1521
                     /locus_tag="PK348_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXK65677.1"
                     /translation="MTILKLENVSLSLDEKPILNNLSVDFWEGHIHAIVGPNGAGKST
                     FSYVVMGISGYRNITGTIYFKGQAINNLTVYERARLGISLGWQEPARFEGLTVAQYLK
                     ASKKDKGMSIEEAMDVVGLSPQEYLQRACDKTLSGGERKRIELASLLVMQPELVLLDE
                     PDSGIDVEALERIFDAINILKERGSTVITITHSMAVLKKAEHAFLMCHGNIIDKGSID
                     KISGYFENKCIPCDHKNIPVLGEVQ"
     gene            1521..2459
                     /locus_tag="PK348_05385"
     CDS             1521..2459
                     /locus_tag="PK348_05385"
                     /inference="COORDINATES: protein motif:HMM:NF013614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HXK65678.1"
                     /translation="MDDKREIAKTLYKTMGLTDAALNDPDIAHLVINGNMVVGAHLVP
                     GLVVEPHETPNGVTVTMKVLAGSIIQKPVHLCFGMLPEDGLQEIIMDVTIEEHAKVSI
                     LAHCTFPNAKNITHKMQANIVIKEGAYYSYVERHVHAPGGLITVVPHAIVTVEKNATF
                     KTDFELLRGKAGIININYETTCYEGSLLEMNAKVNGIDDDDITIRETGHLKGQGAVGV
                     LTTRIAVRDNAKATVYNTLTAHAAFARGHVDCKEIIQDNGVAKAIPIVEVYHPKAHVT
                     HEAAIGSVDDKQLQTLMARGLDEDEAVDLIIQGLLS"
     gene            complement(2463..2894)
                     /locus_tag="PK348_05390"
     CDS             complement(2463..2894)
                     /locus_tag="PK348_05390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65679.1"
                     /translation="MKFDDSKIYFYFSIAVLVAGILFGLPGIYSKIVTEPAIEKLLAQ
                     NTDSQKLKQAYIMLRNPHIFAGYDRYDEAGAGIEYILKEFDNRVAQQKEFTSNDAMYL
                     ELLLVRRQQGSDLSIKTMIYFILLSLLGVTALAIEKHVNKK"
     gene            complement(2891..3352)
                     /locus_tag="PK348_05395"
     CDS             complement(2891..3352)
                     /locus_tag="PK348_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65680.1"
                     /translation="MIKYIIPLLLVVCAGCTQYVDVGDVEFSVQVVFQQNVPVFATTY
                     TITLLNTSINKVVKNYTATVAIVTEDKHTLKQLTFATDKLLPKQVQQITITQNESEEA
                     IKPLFNELEIDINEVIKQQEAALVLPEGMVKVEKAHYETVDIVTYLQEVGQ"
BASE COUNT         1078 a          598 c          676 g         1090 t
ORIGIN      
        1 ctatataata gggattgcca acctttacag agtgctttat aaccttttgt tttatttcaa
       61 ggagtatatc ttttaaaagc tttggatcac ggggaatatc aatattgtta tacaggcatg
      121 ccagctcagg caatgatatt tcgctgtgaa taccaccttt ttccttaaaa aatgaatgaa
      181 taaggtcaag aagctcatac ccaaattgca taaacttatc aggtgaatcg ggcatgatga
      241 atagcttgcg tagatgctct aaatcggtgg gtatcttgcc aggggtatga tctattgtgg
      301 gctcagccaa tctcattgtg gtaaccactc atcataaggt tggaggacat cttattacac
      361 aaaagtaaat ataattaaat taatcaagta cttttgctga aatatacaaa attattacca
      421 gtacctatac aaattgtgca tacattggcg atagttccat taccagtcca ctgacaatat
      481 agccctaaat tgaaaaataa ccaatttttt ggtttttcat tctttcaata ccaattattg
      541 agttttttta aattctctct tgacgatgat acaatctata attaccatga tgaaagtttt
      601 atacgttctt tttatatttg cgttactcaa atgacaagtg gtcatccgtg attcaactgg
      661 cgatttccag ttcatatcaa atgtaagaat tacagtatga cgctcagcat aatggattgc
      721 tatgtttaaa aaattcggga ttcaggacga cagtgttata aattttattt tgataggaga
      781 acagctacaa tgactattct gaaactggaa aatgtttcat tatcattaga tgaaaagcct
      841 atacttaata acctttctgt tgacttctgg gaagggcata tccatgcaat tgttggccct
      901 aatggtgcag gtaaatcaac attttcatat gttgttatgg gcattagtgg ttatagaaat
      961 attactggta ctatttattt taaagggcag gcaataaaca atctcactgt gtatgaacgt
     1021 gcccggctgg gcataagcct gggatggcag gaacctgccc gatttgaagg acttaccgtt
     1081 gcacaatacc tgaaagcatc aaaaaaagat aaaggcatgt ccattgaaga agcaatggat
     1141 gttgtgggac tatcaccaca ggaatacctg cagcgtgcat gtgataaaac cttgagtggc
     1201 ggtgaacgaa aacgcattga attagcttct cttttagtaa tgcaacctga gcttgtgctg
     1261 cttgatgaac ctgattcagg cattgatgtg gaagcgctgg aacgtatctt tgatgcaata
     1321 aatattttaa aggaaagagg ttctacagta ataactatca cccatagtat ggcagtactg
     1381 aaaaaagctg agcatgcgtt tttaatgtgt catggtaaca ttattgataa gggaagcatt
     1441 gataaaattt caggatattt tgaaaataaa tgtatcccct gtgatcataa aaatattcct
     1501 gtgttaggag aagtacagta atggatgata agcgcgaaat tgcaaaaaca ttatataaaa
     1561 caatggggct tactgacgct gccttaaatg acccggatat tgcacatctg gttataaacg
     1621 gcaatatggt ggttggggca catttagtgc cagggcttgt tgttgagcca catgaaactc
     1681 caaacggtgt tactgttacc atgaaggtgt tagcaggtag cattatccaa aagccagtgc
     1741 atctttgttt tggcatgctt cctgaggatg gccttcaaga aataatcatg gatgttacca
     1801 tagaagagca tgcaaaagtt tctatacttg cacactgcac attccccaat gcaaaaaata
     1861 ttacacacaa aatgcaggca aatattgtta ttaaagaggg agcgtattac tcatatgttg
     1921 agcgccatgt ccatgcacca ggaggtttaa ttactgtagt tccgcatgcg atagttactg
     1981 ttgaaaagaa tgccacgttt aaaactgatt ttgagctttt acggggaaaa gctggtatta
     2041 ttaatattaa ttatgagaca acctgctatg aaggaagcct tttagagatg aatgccaaag
     2101 taaatggtat tgatgatgat gatattacca tacgggaaac aggacatctg aaaggccagg
     2161 gtgctgttgg tgtactaacc acacgcattg ccgttcgaga taatgcaaaa gctactgtgt
     2221 acaatacatt aaccgcacat gcagcatttg cacggggaca tgttgattgc aaagaaatca
     2281 ttcaggataa tggtgttgca aaagccattc ctattgttga ggtgtatcat cccaaagcgc
     2341 atgttaccca tgaagcagcc attggcagtg ttgacgataa gcaattgcaa acgcttatgg
     2401 cacgaggtct tgatgaagat gaagcggtgg atttgattat tcagggattg ttgtcgtaat
     2461 agttattttt tatttacatg tttttctata gcaagtgctg taacacccaa taatgaaagc
     2521 aatataaaat atatcatggt ttttatagaa agatcacttc cctgctgacg acgtacaagc
     2581 aacagttcaa gatacatggc atcattagaa gtaaattctt tttgctgggc aacgcggtta
     2641 tcaaattctt tcaaaatata ctcaatgcct gctcctgctt catcataacg gtcatatcct
     2701 gcaaaaatgt gtgggttgcg cagcataatg taagcctgtt ttaatttctg gctatcggta
     2761 ttctgtgcta aaagcttttc tattgctggt tcagttacta tttttgaata gatacctggt
     2821 aatccaaaca aaattcctgc aaccagtact gctattgaaa aataaaaata tattttgcta
     2881 tcatcaaatt tcattgtcca acctcttgta aatatgttac gatatcaacg gtttcatagt
     2941 gtgctttttc cacttttacc ataccctcgg gcaacaccaa agctgcttcc tgctgtttta
     3001 ttacttcatt aatatctatc tccaattcat tgaatagtgg tttaattgct tcttcagatt
     3061 cattctgtgt aatggttatc tgttgtactt gctttggcaa aagtttatca gtagcaaatg
     3121 ttaattgctt aagagtatgc ttatcttcag taactatcgc cactgttgcc gtgtagtttt
     3181 ttacaacttt atttatactg gtgtttaata gggttatggt ataggtagtg gcaaatacag
     3241 gtacattttg ctggaaaacc acctgtactg aaaactcaac atcacccaca tcaacatact
     3301 gtgtgcagcc agcacataca acaagtaata gcgggattat gtattttatc attagtacct
     3361 caccaacata tattgtgccg ttattgtcct attacactga tttagtcaag taaaatgtgg
     3421 aaaggtagat ttaataaaat aa
//
LOCUS       DAOSZP010000201         3429 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_21900_length_3429_cov_3.648192, whole genome shotgun sequence.
ACCESSION   DAOSZP010000201 DAOSZP010000000
VERSION     DAOSZP010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3429)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3429)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3429
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21900_length_3429_cov_3.648192"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..753)
                     /locus_tag="PK348_05400"
     CDS             complement(<1..753)
                     /locus_tag="PK348_05400"
                     /inference="COORDINATES: protein motif:HMM:NF024827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HXK65681.1"
                     /translation="MKSKIVIIISMVALTVYAFFTGCKSTPHYTLAQFISPATCGGCH
                     DEIYEQWKNSMHNLAQKDIIYKEYAAFYLDDIKKQNPVNKDELEEAESCVKCHIPVGF
                     ITGKPVTVTEELAMENELPEITKEGIQCDYCHVIIDAKKNYNAHFTYKPGNGEADPGV
                     KYGPFKDATPDWHQAAYSKFHTQAAFCGTCHDVRHVVYNTKLETTFEEWSKSPYNTGD
                     EKTTVPCQGCHMYHRPGVPATGSTPRPNNPGFA"
     gene            complement(929..1960)
                     /locus_tag="PK348_05405"
     CDS             complement(929..1960)
                     /locus_tag="PK348_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HXK65682.1"
                     /translation="MDLLQEYFFIFIPVVYFGVAFTVLLLFKKIVFSLLTKWAAATSW
                     DIDDIIIDGLKKPAFFVVLALAILIASQYTILSEKWQMLISKSVHVIIIFALTLGIAN
                     IVGSLLQKYVKIANIPLAPTGLTYIIIKGLFVLIGILIILNYLGISIAPILTTLGVGG
                     LAVALALQDTLSNLFAGMQILIERSVRVGDFVKIDEGIEGYVEDITWRTTRIRMLPNN
                     IVIVPNSKMAQSMITNFYLPVPHLALRIPVSVSYNSDPQHVEHVILDEVKKSASTIQQ
                     LVMDPEPVVRFIPGFGDSSLDFTLIVYVKEYADQFPVQSELRKRIFNRLKEEGIEIPF
                     PQRVVHIVK"
     gene            2153..>3429
                     /locus_tag="PK348_05410"
     CDS             2153..>3429
                     /locus_tag="PK348_05410"
                     /inference="COORDINATES: protein motif:HMM:NF017021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HXK65683.1"
                     /translation="MKKILKWVAIVIGSIFALIIIASIVLMLVVTKDMIAQQMEKALN
                     RHVTIEKIDVSIFSVVSGIEVKGVAISNFKTPKQLEALKDKPVDKGDLFVGLDSFTFK
                     LKFLPLLQGKFELRELLLTGPKINITRYQSGAFNFSDLMKPSKEEKKVEEVKKEEPSK
                     PLTADTLPISITIGKVGIEKGTITFVDQSLQQTFQLYNLNALVHDIEIDPKDLAKKNQ
                     VKLKLDIGIKTIGKTSGGSVESFDIGLASRGTVKPFDLKTRILNPEIALKAGSPYGTM
                     TGLQIFDAIKSNETLANYAGKFDFLSKDVKWKNGYVDIWYKGGLLKITNGKISTDDYA
                     GNFKADVNFNTKAVNADIDMTLNEKHNKSIYNGIEKNASKLIKGDVAKYVKPDKVAEK
                     AFGYLTNKEGKVFLQFLVTGTMDNPKTRLVAPKL"
BASE COUNT         1123 a          648 c          635 g         1023 t
ORIGIN      
        1 agcaaatccg ggattgttgg ggcgtggagt agatcctgtt gccggaacgc ctggcctgtg
       61 atacatatga catccctgac aggggacagt ggttttttca tcgccagtat tgtatgggct
      121 tttgctccac tcttcaaagg ttgtttccag ttttgtatta tagactacat gcctaacatc
      181 atggcatgta ccacagaatg cagcctgcgt gtgaaatttg gaatatgctg cctgatgcca
      241 gtcaggtgtt gcatccttaa aagggccata ctttacaccg ggatctgctt caccattgcc
      301 tggcttatag gtaaaatgtg cattatagtt tttctttgca tcaatgatta catggcagta
      361 atcacactga attccttctt ttgttatttc aggcaattcg ttttccattg ccagttcttc
      421 agtcactgtt actggtttgc cggtaataaa gcctaccggt atatggcact taacacagct
      481 ttctgcctct tcaagttcat ccttatttac aggattttgt ttttttatat catctaaata
      541 aaatgcagca tactctttgt agattatatc cttttgtgca aggttatgca ttgagttttt
      601 ccactgttca tatatctcat catggcaacc accacaggtt gcagggctta taaattgtgc
      661 taatgtgtag tggggtgttg atttacatcc ggtaaaaaaa gcatatacgg ttaatgcaac
      721 catgctgatt ataatgacta tttttgattt catggaactg tctcctcaaa actatgattt
      781 acggatacta tgccatcctg aggaatcccg acctgtcggg gtgacgaaga atcttgccgt
      841 ttaaaaatcc agattcttct cctgatttat cgggatacaa aatgtaatta atacttctgt
      901 agtaaatcaa aattgtcaac aatgtttatt attttacaat gtgaacaact cgctggggga
      961 atggtatctc aataccttcc tcctttaagc ggttaaatat tcgcttacgc aattcagact
     1021 gaacgggaaa ctggtcagcg tattctttta catacactat aagggtaaaa tccagggaac
     1081 tatcgccaaa acctggaata aaccgtacta caggctctgg atccataaca agctgttgta
     1141 tggtgctagc tgattttttc acctcatcaa gtataacatg ttcaacatgc tgagggtcgg
     1201 agttatagct cacacttact ggaatcctca gcgccagatg tggcaccgga agataaaaat
     1261 tggtaatcat gctctgagcc atcttactgt tgggaacaat aacaatgttg ttaggcagca
     1321 ttctgattcg tgtagttcgc caggtgatat cctcaacata tccttcaatg ccttcatcaa
     1381 tttttacaaa gtctccaacc cttaccgacc tttcaatcaa aatctgcatc cctgcaaaaa
     1441 gatttgacag tgtatcctgc agtgcaagag caactgccag accacctact ccaagagttg
     1501 tcagtatagg agctatcgat atacctaaat agttgaggat gattaaaatc cctataagta
     1561 caaataaacc cttgatgata atataggtta agccagtagg tgccagcggt atatttgcaa
     1621 tctttacgta tttctgtaat aatgatccaa ctatattagc aatgccaaga gtaagagcaa
     1681 aaataataat aacatgtaca cttttactta tcagcatttg ccatttttct gaaagtattg
     1741 tgtactgtga agcaatgaga atggcaagag caagaacgac aaagaatgca ggttttttca
     1801 atccatcaat aattatatca tcaatatccc agcttgtagc ggcagcccat tttgttaaaa
     1861 gcgaaaacac tatttttttg aacaataaca aaacagtaaa agcaacacca aaatacacta
     1921 ccgggataaa aataaaaaaa tattcttgta gtaaatccat ggtttactcc tctgactatt
     1981 gaattaccaa aaatataaca ttaccaatct ttataaaata aatcataaaa attgtattga
     2041 tttttctatt agtattgcaa taataatttt ttattaaata tcctgtaaca aatcatttcc
     2101 caatacaacc tcttttccta ttgaggtata tactattttc aggaggatat ctatgaaaaa
     2161 aatacttaaa tgggtggcga tagttatcgg gagcatattt gcactgataa ttattgcaag
     2221 cattgtactt atgctggttg tgactaaaga tatgattgcc cagcagatgg agaaggcact
     2281 gaaccgtcat gtaaccattg aaaagattga cgtcagtata ttcagtgttg tttcagggat
     2341 tgaagtaaag ggtgttgcca tatcaaactt caaaacaccc aaacagttag aagccctcaa
     2401 agacaagcct gtagataaag gcgacctttt tgttgggctt gactctttca catttaaatt
     2461 aaagtttttg ccacttcttc agggtaaatt tgaactccgt gaacttttgc taacaggccc
     2521 aaaaataaat atcacccgct atcagagtgg agcattcaat ttttctgatt taatgaagcc
     2581 atctaaagag gaaaagaagg ttgaagaagt taagaaagaa gagccttcaa aacctctaac
     2641 cgctgatacg ttaccaatca gtataacgat agggaaagtt ggtatagaaa aaggcacaat
     2701 cacttttgtt gaccagtcac tgcagcaaac attccagcta tacaatctca acgcgcttgt
     2761 acatgatatt gaaatagacc ctaaagacct tgcaaagaaa aatcaggtaa agttaaaact
     2821 ggatattggc atcaaaacta taggcaaaac aagtggtggc agtgtagaat cctttgatat
     2881 tgggttggcc tcacgtggaa cagtaaaacc ttttgacctg aagacacgta tattaaaccc
     2941 tgaaatagca ttaaaagcag gcagtcctta cggaacaatg actggtttac agatatttga
     3001 tgctatcaag agtaatgaaa cactggctaa ttatgcaggc aagtttgact ttttatcaaa
     3061 agatgtaaaa tggaaaaatg gatacgttga tatctggtat aaaggcggat tattaaaaat
     3121 caccaatgga aaaatcagca ccgatgacta tgcaggcaat ttcaaagctg atgtaaattt
     3181 caacacaaaa gcagtaaatg ctgacattga catgacactc aatgaaaaac acaataaatc
     3241 catttacaat ggaattgaaa agaatgcatc aaaactcatc aagggcgatg ttgcaaaata
     3301 tgttaaacca gataaggttg ccgaaaaagc ttttggctat cttaccaaca aggaagggaa
     3361 agtattttta cagtttcttg taaccgggac aatggataat ccaaaaacca ggcttgttgc
     3421 cccaaaact
//
LOCUS       DAOSZP010000202         3398 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_22124_length_3398_cov_3.406820, whole genome shotgun sequence.
ACCESSION   DAOSZP010000202 DAOSZP010000000
VERSION     DAOSZP010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3398
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22124_length_3398_cov_3.406820"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            77..2050
                     /locus_tag="PK348_05415"
     CDS             77..2050
                     /locus_tag="PK348_05415"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HXK65684.1"
                     /translation="MYKKIICGIIIFCTIPSLYISCNQKRTPYKPKAIEGVLDLRSWD
                     ESSNQVISLDGDWEFYWNQLIEPSQFTKDLQPFTYIEVPKAWNKQGNFRYSFPAFGYA
                     TYRLHIYHDPKYVGQIKTLIMPYVHTAYTLWVNGKIISQNGKVGTSRSSMIPFQLPVI
                     TQFVINSTVTEVVLHISNFQQRTGGILRSIKYGNYNIINKQYELDLFITLFVTAALFI
                     ILLYHLGLFLVNKGYKPNLYFAFFCAIIGFRLLLTDEKLFVKIVPSLPWDMYLRMEYS
                     TLLFAVISFAHFIDGLYPRMFNTIILRAIDIYFCLFFIFVLVVPISYVSYALIFIQIG
                     LIVVGVYLLFIMIRATSTETTNSFIYISGNIILFFTLINDILYTQQLINSIYLFEIGL
                     LVFVFTQSLAALEESIKIQNRLIAINSELEIARRIQKTILPEAVPSTHSIHVEACYIP
                     MDEIGGDFYHFYELDEERLGIIIADVTGHGIPASLIASTVNIAFSLQRNHADNPATLL
                     ANMNTILYGKTGGQPITALYAYIDTSQKVVKFSRAGHPLPLIWRSSSNTITEIEIPGK
                     ILGVFKTIKNDITTYPIYSNDRIILYTDGITEIRNNRNEIFGIERFIACIEKNHHKST
                     RDLIDSIVDAVIQWAGSKNKITDDITLVIVEIK"
     gene            2275..2514
                     /locus_tag="PK348_05420"
     CDS             2275..2514
                     /locus_tag="PK348_05420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65685.1"
                     /translation="MSNHKHSTKQQYWEEYLYHYEASGMSKAAYCRENNLVYHQFVWW
                     HKRLCSQLKEGAEPFVALQHKLVIPTHRESGIVIE"
     gene            complement(2643..3095)
                     /locus_tag="PK348_05425"
     CDS             complement(2643..3095)
                     /locus_tag="PK348_05425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65686.1"
                     /translation="MNIETTTCIAYEHLHLLSQYASIHKLSLHTFIINFINFVMSYKK
                     IPVKTCRRLQYRNRYHSWKRVHLYLLEHEYEFVMDVRKVCKMSLAKVIAYCVDNYLFD
                     FLAALDSDDNTDNYRCSGYTFSFYLEDGIQCCQFYWGPPPQLLKQATQ"
BASE COUNT         1155 a          537 c          616 g         1090 t
ORIGIN      
        1 tactttttta atttatatga aaatcttagt gaatattaat taatataact attgaaaatt
       61 tgggttatta tatccaatgt ataaaaaaat tatttgtggt attattatat tttgtactat
      121 accatcatta tatatcagct gtaatcaaaa aagaacccct tataaaccca aggcaataga
      181 gggcgtttta gacctgcgga gctgggatga atcatcaaac caggttatat cattagatgg
      241 ggattgggaa ttctactgga atcaacttat tgaaccttct cagtttacaa aagatttaca
      301 accatttacc tatattgaag ttcctaaagc atggaataaa cagggaaatt tcaggtattc
      361 atttccagct tttggatatg ctacctatag actgcatatt tatcatgatc caaagtatgt
      421 agggcaaatt aaaacattaa ttatgccata tgtacatacg gcgtatactc tatgggtaaa
      481 tgggaagatc attagccaga atggaaaagt tgggacttcc aggtcttcaa tgataccttt
      541 tcagttgccg gttataacac aatttgtcat taattccaca gttactgaag tcgttttgca
      601 tatatctaat ttccagcaac gtactggggg aattttacga agtatcaaat atggaaatta
      661 taatataatc aataaacaat atgaattaga ccttttcatc acactgtttg tcacagcagc
      721 cctttttatt attcttttat atcatcttgg tctattttta gtaaataaag ggtacaaacc
      781 aaacctttat tttgcttttt tctgtgcaat tattggattc aggctattac tgactgatga
      841 aaagcttttt gtaaaaatag tcccatcttt accatgggat atgtatttac gaatggaata
      901 ctcaacttta ttgtttgcag taataagctt tgctcacttt attgatggcc tatacccaag
      961 aatgtttaat accataattt taagggctat tgatatatat ttctgtttat tttttatttt
     1021 tgtattggtt gtacccatat catacgtttc gtatgctctt atttttatcc agattggttt
     1081 aatagtagtt ggtgtatatc ttttatttat aatgatacgg gcaacatcaa ctgaaacaac
     1141 aaactctttt atttatatat ccggtaacat catattattt tttacactta tcaatgatat
     1201 attatatacc caacagctga ttaatagtat ctatctgttt gagattggtt tattagtctt
     1261 tgtatttact caatcattgg ctgcgttgga agaatcaata aagattcaaa atagattaat
     1321 tgcgatcaac agtgagcttg aaattgcacg gcgcatacaa aaaactattt taccagaagc
     1381 agtgccatcc acacattcta ttcatgttga ggcatgttat atacccatgg atgaaatagg
     1441 tggcgatttt taccattttt atgaattaga tgaagaaagg ttggggatta ttattgctga
     1501 tgttaccggg catggtattc cggcatcttt gattgcatca acagttaata ttgcattttc
     1561 attacaaagg aatcatgccg ataatcctgc tacacttctg gctaatatga ataccatatt
     1621 atatggtaaa acaggtgggc aacccattac cgcactatat gcatatattg atacatcaca
     1681 aaaagtagta aaattctcac gggcaggaca tccacttcca ttaatctgga ggagcagtag
     1741 taatacgata actgagattg aaattcctgg aaaaatactg ggtgttttta aaactataaa
     1801 gaatgacatt accacatacc caatatatag caatgacaga ataatactgt atactgatgg
     1861 aattacagaa atccgtaata atagaaatga aatatttggc attgagcgtt ttatagcatg
     1921 tattgaaaaa aatcatcata aaagtacaag agatcttatt gatagcattg ttgatgcagt
     1981 tatacaatgg gcgggaagca agaataaaat aactgatgat attactttag tcattgtaga
     2041 aattaaataa agtcacttaa agctatctaa tggtaattgc atctatttcg atgggagcac
     2101 cagcaagcag atcaaagact caagatgcag gtatgcgcag gggaatttga aggaaaaata
     2161 atttttatat atttcattca ttgtatccgt caaattaaca ccaacttcac aaaagaataa
     2221 agatatgcat acactccgta tgtaaagcat acaatgagca tacggaagta aacaatgagt
     2281 aaccacaaac atagtacaaa gcaacagtac tgggaagagt atctgtatca ctatgaagca
     2341 agtggcatgt caaaggcagc gtattgcagg gaaaataatt tggtgtatca tcaatttgtg
     2401 tggtggcaca aacggttgtg ctctcaatta aaagaaggtg cagagccatt tgtagcattg
     2461 cagcacaagc ttgtaattcc cactcatcgt gagtcaggta tagtcataga atgacggatg
     2521 ctgcaagtaa aactaccagt ggaaagcagc agaaaaccgt gaagacagta cttaaagcta
     2581 cgtatccaag aggggatacg gttttcttct ttaggtgggg taataacagt agtggtactg
     2641 cgttattggg ttgcctgttt tagtaattga ggcggtggtc cccagtagaa ttggcaacac
     2701 tggatgccgt cttcaagata aaaggagaat gtatagccac tacaacgata gttatcggta
     2761 ttatcatcac tatccaatgc agcaaggaaa tcaaaaagat agttatcaac acaataggca
     2821 ataaccttag ctaaagacat cttgcacacc ttacgcacat ccatcacaaa ttcatactca
     2881 tgctcaagca aatacagatg cacccgcttc cagctatgat agcgattacg gtattgtagt
     2941 cgtctgcagg ttttcaccgg aatcttttta tatgacatca caaagttaat gaaattaatg
     3001 atgaacgtat ggagcgatag tttatggata gaagcatact gtgataaaag gtgtagatgt
     3061 tcataggcaa tgcaggtagt cgtttctatg ttcatgaaaa cctccacagt agtgataggg
     3121 taacagtaaa atgaaagaaa gaaaatgcaa gaaaaaatta tatatgatat agtacatagc
     3181 acagcagatg tgaagttcag cagtaactat cgcaacaaga aaaacagaaa ttttacttcc
     3241 aattgatgcc catatgaagt aagtgctatc ctgtggttaa tttgacggct acattttaac
     3301 aatattctgg attctgtaga tgctataata gttttttttc ttctaatttt ttctgaatgg
     3361 attgtagttg cttgtagtta ttaataattt tgatcata
//
LOCUS       DAOSZP010000203         3397 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_22130_length_3397_cov_3.624177, whole genome shotgun sequence.
ACCESSION   DAOSZP010000203 DAOSZP010000000
VERSION     DAOSZP010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3397
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22130_length_3397_cov_3.624177"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..702
                     /locus_tag="PK348_05430"
     CDS             <1..702
                     /locus_tag="PK348_05430"
                     /inference="COORDINATES: protein motif:HMM:NF015113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HXK65687.1"
                     /translation="RMIFDTQVKHIVNGITGANEKDKHYKGVNPGRDFVIKEEADITR
                     VVAGDKCPHCGYPLKEKKGIEVGHIFKLGYKYTKSMNVTVLDQNGKAITPIMGCYGIG
                     VNRTMAAVIEQHHDERGIIWPMSVAPFHVHCVSLAKNEAEEAMADEVYSMLLKAGIEV
                     LYDDRKASPGFKFGDADLIGIPIRITLGKAFFGDSSSIEVKLRNQDEKVIIAKDSIVT
                     YCLNLIAESLKTLES"
     gene            735..884
                     /locus_tag="PK348_05435"
     CDS             735..884
                     /locus_tag="PK348_05435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65688.1"
                     /translation="MGEAITVSILVILALVYSIQYVVAKFTKPQQCDCTQCPIADCKE
                     RKRTV"
     gene            complement(1055..1408)
                     /locus_tag="PK348_05440"
     CDS             complement(1055..1408)
                     /locus_tag="PK348_05440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HXK65689.1"
                     /translation="MSKPIDLEGRVTHDPILRKTKTGKSVCIFTIAMDHPVKGETKPH
                     VSFIDIETWEKLADLCAENIRKGKKIQATGRLKQDRWQDDTGKPRSKIKLVSNQVIFI
                     EPLNSKHNEEVAIES"
     gene            1681..>2592
                     /locus_tag="PK348_05445"
     CDS             1681..>2592
                     /locus_tag="PK348_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008083070.1"
                     /note="catalyzes the removal of amino acids from the N
                     termini of peptides; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HXK65690.1"
                     /translation="MAEEKKSPNQELQEKISVKKESAWAQLKDKVDSIYSFGEEYKLF
                     IGKNKTERLCIRTMVEMLEKAQFSSINIKGTLSPGDAVYKIIKGKSLLACKVGSDPMK
                     VRIIGAHVDSPRLDLKPYPLYEDAELALLKSHYYGGIKKYQWVNIPLAIHGVAFTKEG
                     KTVEIHIGENPDEPRFIIPDLLPHLAKDQMKKTGETIIEGEDLNVIFAHIPVDDKDIK
                     EKIKFNVLKYLHSTYGLIEEDFIAADIELVPALQPVDIGIDRGLIAAYGQDDRACAYT
                     AFRALLDAQDCKETVLLYLADREEIGSM"
     assembly_gap    2593..2602
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2603..3078
                     /locus_tag="PK348_05450"
     CDS             <2603..3078
                     /locus_tag="PK348_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015283738.1"
                     /note="catalyzes the removal of amino acids from the N
                     termini of peptides; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HXK65691.1"
                     /translation="VFVNFMREYIRKTGITATWDELLENALSISADVTGALDPTFKSV
                     NDPNNVSYLGHGVSIEKYGGRGGKYSTSEAHAEYMHYLRSLATKHGIPWQTGEQGKID
                     IGGGGTIAMFMSRYGMDCVDVGPCMLGMHSPFEVTSKVDIYCTYLLYKAFFEDAR"
     gene            complement(3093..>3397)
                     /locus_tag="PK348_05455"
     CDS             complement(3093..>3397)
                     /locus_tag="PK348_05455"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HXK65692.1"
                     /translation="TKHYSFYGKRLNLYYWQDIHGKEVDFIIEHASGKIIAIECKLTE
                     NPTIKDCNNIFKLIDYYGEDRVSTSYIVCNTPTPYQIDKKIYATNIGELLSTLLKQ"
BASE COUNT         1042 a          560 c          722 g         1063 t
ORIGIN      
        1 cgtatgatct ttgatacaca ggtaaaacat attgtgaacg gcattaccgg agccaatgaa
       61 aaagacaaac actacaaagg agtaaacccc ggcagagatt ttgttatcaa agaagaagct
      121 gatattacac gtgtggtagc gggagataaa tgtcctcact gtggttatcc acttaaagaa
      181 aagaaaggta ttgaggtggg gcatattttc aagctgggat ataaatatac taagtcaatg
      241 aatgtcaccg tgcttgatca aaatggtaag gcaatcacac caattatggg ttgttacggg
      301 attggtgtta accgaaccat ggcagcagta attgagcagc atcatgacga gcggggaatt
      361 atatggccaa tgagcgttgc gccgtttcat gtacattgtg tgagcctggc aaagaatgaa
      421 gctgaagagg ccatggccga tgaggtgtat tctatgttgc tcaaagctgg catagaggtg
      481 ctgtatgatg accgtaaggc cagcccggga tttaagtttg gtgatgctga tttgattggt
      541 attcccatcc gcattacgct gggcaaggca ttttttggcg atagttcctc tatagaagtc
      601 aaactgcgaa accaggatga aaaggtaatc attgctaaag attctatcgt cacatattgt
      661 ctgaatctga ttgccgagag cttgaaaacg ttagaatcat agatgtatgt gtgtgatgtg
      721 atatacttat cttcatggga gaagctatca cagtaagcat actggtaata ctggcattag
      781 tgtatagtat ccagtatgtt gtagcaaaat ttacaaaacc acagcaatgt gattgtacac
      841 agtgccccat agctgattgc aaagaaagaa aaaggactgt ctaaatgatt gttttgatct
      901 gagtttcttt gcagttacac agttgatttt atcatacaat gtggcaaaac tgtattacca
      961 tgagtttgtc gtccataatt atggttacac aattcaagta acaaccgttt tttagacagc
     1021 cctgaaaaat ttttaataaa agagtctgct atagttacga ttctatagct acttcctcat
     1081 tgtgtttgct atttaatggt tcaataaata taacctggtt tgagacaagt ttgatctttg
     1141 atcggggttt gccagtatca tcctgccatc tgtcctgctt taatcgacct gttgcctgaa
     1201 tctttttccc ttttcgtatg ttttctgcac acaaatcggc taacttttcc catgtttcaa
     1261 tatcaataaa agaaacatgg ggttttgttt cacccttaac cggatgatcc atagctatgg
     1321 taaaaatgca cacagattta ccggtttttg tttttcgtaa aatgggatca tgagtaactc
     1381 ttccttcaag atcgattggt tttgacatag tatcacctct ttttagtaga atttttggta
     1441 agacgtttca ttatatacaa cgcatgaagt tggaaaaatt aacgaatgaa tgaaaaattt
     1501 ttttaaaaaa atgaaaaatt tttactattt ttctttataa gctctaacgc agcatagaaa
     1561 ctgtggaatg aatattcttt gcacaaactt tttgatttat aaaaaatata attctgttga
     1621 ccttttgtat gctttaccca tgttgtattg tagatgttta aattttttgg gaggatatat
     1681 atggctgagg aaaagaaatc acccaatcag gaattacagg aaaagataag tgtaaaaaaa
     1741 gaaagcgcct gggcacaatt gaaggataag gttgatagca tatacagttt tggggaagaa
     1801 tataaattat ttattggcaa gaataagacg gagcggctgt gtattcgcac catggttgag
     1861 atgcttgaga aggctcaatt ttcttctatt aatataaaag gtaccttaag ccctggtgat
     1921 gcagtatata aaattataaa aggcaaaagt ttactggcct gtaaagtagg ctctgacccc
     1981 atgaaggtgc gcatcattgg cgctcatgtg gattcaccac ggcttgattt aaagccgtat
     2041 cctctgtatg aagatgctga gcttgcactt ttaaaatccc attactatgg tggcataaaa
     2101 aaatatcagt gggtcaatat accgcttgct atacatggtg tggcattcac aaaagaagga
     2161 aaaacagtgg aaatacatat aggcgaaaat cccgatgagc cgcgatttat tattcctgat
     2221 ttattgccac atcttgccaa agatcagatg aaaaaaacag gtgaaacaat catagaaggt
     2281 gaagatctta atgttatctt tgcccatata cctgttgatg ataaggatat taaggaaaag
     2341 ataaaattca atgtactgaa atatctccat tccacctatg gccttattga agaagatttt
     2401 attgctgcgg atattgaact ggtacctgcg ttgcaacctg tggatatagg tattgaccgc
     2461 gggcttattg cagcgtatgg tcaggatgac agagcttgtg cctataccgc atttagggcc
     2521 ttgcttgacg cacaggattg taaagaaaca gtcctgctat atttagctga ccgtgaagaa
     2581 ataggatcca tgnnnnnnnn nnttgtgttt gttaacttta tgcgggagta tatacggaaa
     2641 accggtataa ccgcaacatg ggatgagctt ttagaaaatg ccctttcaat ttctgctgac
     2701 gtaacaggtg cgctggatcc cacattcaaa agcgtcaatg accccaacaa tgtatcgtat
     2761 ttaggacatg gtgttagcat tgaaaaatat ggtggccggg gcggcaaata ttctaccagt
     2821 gaagctcatg ctgaatacat gcattatttg cgcagtttgg ccactaaaca cggtataccc
     2881 tggcaaacag gtgaacaggg taagatagat attggcggtg gtggaaccat tgccatgttt
     2941 atgagccgtt acggcatgga ctgtgttgat gtgggtccct gcatgttagg aatgcattcg
     3001 ccctttgagg taacatccaa ggttgatatc tactgtacat atttgttgta taaggctttt
     3061 tttgaggatg ctcgataaag taaaaagatt tattattgtt ttagtaatgt actaaggagt
     3121 tctccaatat ttgttgcata gattttttta tctatttgat atggtgttgg tgtattacat
     3181 acaatatatg atgtggatac tctatcttca ccatagtaat caataagttt aaagatatta
     3241 ttacaatctt ttattgtagg attttccgtt agtttacatt caatagctat tatctttcct
     3301 gatgcatgtt caataataaa atctacttcc ttcccatgaa tgtcttgcca gtaatagagg
     3361 tttaaacgtt taccgtaaaa agaataatgc ttcgtga
//
LOCUS       DAOSZP010000204         3382 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_22247_length_3382_cov_3.576495, whole genome shotgun sequence.
ACCESSION   DAOSZP010000204 DAOSZP010000000
VERSION     DAOSZP010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3382)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3382)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3382
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22247_length_3382_cov_3.576495"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2022)
                     /locus_tag="PK348_05460"
     CDS             complement(<1..2022)
                     /locus_tag="PK348_05460"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HXK65693.1"
                     /translation="MKLSYTFKKRHIVYPVIVVAALYYFIATHPFGIYQAYPDTLSPQ
                     NEGKYTYDIPLDPKSPWPKFRANSLQNGRSAVKPTYRKTLRPWVFKTGKGIFSSPVVD
                     AHGTVYIGSADHYFYAIKQDGSLKWKIKTGEIIDSSALLDDRGFVYVGSGDSYVYCIN
                     RETGTIVWKSKAHSVQEVWEQFTIKTYNVNWFEGNIGMLPDGSIIAPNDNYLVYQIDR
                     QNGKRITQFPANEMIWSLPAVNTKTGKMFFGTQFMALKNVYCYNYRTQKAEWTNGGFG
                     SNAASPLLTNCNVKGMVIVGGYDGYVRAYAQDSGKQLWKQGLRDHIYASPSQTSHGLI
                     IQPCADGTIYALNPENGKIVWAYDTLEPIRSSAAIDGNDIIYVGSGEGKLFCLNPDGT
                     LRWSYLCIDEDRNDLNSSPALGPNGVYIGGENGSVFFIPYDYPLHNAYKDKNCYFGKG
                     EALPNDGAFLVYTSSFGKLLPQPPANIDANQPLAFTLFVRKNGDTLKSSIDRDSLSVS
                     ITGNPKHRIDVSANKQFFVITPQETWVGQDGGTITITIKGQYITGMHRFGLKFFGGSK
                     AGTFHVSYSFAIAPYKGVLPYTYPQITGKPQTVFECKRLAAPNPTMLPSWNQIGFDSL
                     HYVGGVVSKASGGLVVWFIGGKLQDGKTVVDPTLEARYPLYLRSDGKCVT"
     gene            2501..3133
                     /locus_tag="PK348_05465"
     CDS             2501..3133
                     /locus_tag="PK348_05465"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HXK65694.1"
                     /translation="MEKQRARTGSEKEYRKEQLKKAALTLFAQKGFKNTTVEMITKKA
                     HLSPAAFYLYFNNKIAIYRALNKDGIDILEDLMKQALHTSGGSSVDKLYALARAYYNF
                     YREHTDYFYITEILHLGNDEFFYDSAMVKELEKRTLVLLQIVASVIEEGIEKNELRKI
                     DPMKTAVTLWGMIDGVLILEVKKSTLFTGFMIDALIGQLMDMVLLGVKKK"
BASE COUNT         1039 a          621 c          689 g         1033 t
ORIGIN      
        1 agtaacacat tttccatcac tccgcaaata taatggatag cgtgcctcaa gggtggggtc
       61 caccactgtt ttgccatcct gaagttttcc gccaataaac cacacaacaa gcccaccgct
      121 ggctttgctg accacaccgc caacatagtg gagcgaatca aacccaatct gattccacga
      181 aggaagcatt gttgggtttg gcgcagctaa tcgtttacat tcaaacactg tctggggttt
      241 cccagtgatt tgtggatagg tatatggcaa aacaccttta tatggagcta tcgcaaatga
      301 ataggaaaca tgaaaagtgc ctgctttgct acccccaaaa aacttgagcc caaacctgtg
      361 catgccagta atatattgtc ctttgatggt gatggttatg gtgccaccat cctggccaac
      421 ccatgtttcc tgcggggtaa tgacaaagaa ctgtttgttt gcagatacgt caatacgatg
      481 ttttggattt cctgttattg atactgaaag tgaatccctg tcaatacttg atttcagtgt
      541 gtcaccattt ttgcgcacaa agagggtaaa ggcaagtggc tggttagcat caatgtttgc
      601 aggtggttga ggtagcagtt ttccaaacga actggtatat actaaaaagg cgccatcgtt
      661 ggggagagct tcacctttgc caaaataaca atttttatct ttgtaagcat tgtgtaatgg
      721 ataatcatag gggataaaga acacactgcc attttcacca ccaatgtata cgccgtttgg
      781 ccccagtgct ggagagctgt ttaaatcatt gcggtcttca tcaatgcaca gatatgacca
      841 tcgcagggta ccgtcaggat taaggcagaa aagcttgcct tcgccagaac caacgtatat
      901 gatgtcattg ccatcaatag ctgctgacga gcgtatgggc tctaatgtgt catatgccca
      961 cacaatcttt ccgttttctg gattcagcgc atagatagtg ccatcagcgc atggctggat
     1021 gataagccca tgtgaagttt gagacgggct tgcatagatg tggtcacgca ggccctgttt
     1081 ccacagctgt ttaccactgt cctgcgcata tgcgcgcacg tagccgtcat aaccaccaac
     1141 gattaccatc ccctttacat tgcagtttgt aagaagtggt gaagctgcat tggaaccaaa
     1201 accgccattt gtccattcag ccttttgtgt gcgatagtta tagcaataca cattcttgag
     1261 cgccataaac tgagtgccaa aaaacatctt ccctgttttt gtgtttacag caggaagcga
     1321 ccatatcatt tcattagcag ggaactgtgt tattcttttt ccattttggc ggtcaatctg
     1381 atataccaga tagttatcgt ttggggcaat gatactgcca tcaggaagca tgccaatgtt
     1441 gccttcaaac cagttcacgt tgtatgtttt tattgtaaac tgctcccaca cttcctgaac
     1501 actgtgagct ttacttttcc atacaatggt gccagtttct ctgttgatgc aatacacata
     1561 actatcgcca ctaccaacat acacaaaccc cctgtcatca agaagcgctg atgaatcaat
     1621 gatctcccct gttttgattt tccatttaag ggaaccatcc tgcttgatgg cataaaagta
     1681 atggtcagca gaaccaatgt atacagtacc gtgagcatca acaactggtg agctgaatat
     1741 gcctttacct gttttaaata cccacggacg cagtgttttt ctatacgttg gtttcacggc
     1801 gcttcttccg ttttgaaggc tgtttgctct gaattttggc catgggcttt ttgggtcaag
     1861 gggaatgtcg taggtatatt ttccttcatt ttggggcgat agtgtatcgg gatatgcctg
     1921 gtagatgcca aagggatggg ttgcaataaa ataatatagt gcagcaacta caatgacagg
     1981 atatacaata tggcgctttt taaatgtgta cgatagtttc attggtttac cttgttaaat
     2041 atatagttta taatgaaaaa tttattaata ctaatggcct ggacgaaata cccaaataac
     2101 ttctttctga aaatgcaata gcaatgtata gctatactat aattaaatac cccataaaaa
     2161 taactaaata gtcatttatt tattttgtca acacaaaatt tactgtaatg gtattgattt
     2221 ttatgccata tatactcata attgaaattt gttaacaaag taataaataa tacacaacat
     2281 ctctgtgttt agaaagattt tgcattaata tcataataat ggtgtatggt agtataactg
     2341 tgtttgatgt ttgcaatatg aaaaaaagga aatattttca ttgcaagtta tcatgaacta
     2401 ctcttgtatt gtgacaggtt ctattgggga attcacggta tgcaatggat tcccagatat
     2461 gttgggaaaa catatattaa ttatatggta gttgaaaaaa atggaaaaac agcgtgcgcg
     2521 cacaggaagt gaaaaggaat accgtaaaga gcaactaaaa aaagctgctc taacattatt
     2581 tgcacaaaaa ggttttaaaa acaccacagt tgagatgata acaaaaaagg cacatttaag
     2641 tccggcggca ttttacctgt attttaataa taaaatagcc atataccgtg cattgaataa
     2701 ggatggcatt gatattcttg aagatttaat gaaacaagca ttacatacgt caggtggtag
     2761 cagtgttgat aaactatatg cacttgccag agcatattat aatttttaca gggaacatac
     2821 ggattatttt tatataaccg agattcttca tcttggcaat gatgagtttt tttacgatag
     2881 cgcaatggta aaggagcttg aaaaacgtac gcttgtatta ttgcaaatag ttgccagtgt
     2941 cattgaagaa ggtatagaaa agaatgaatt gagaaagatt gatcccatga aaacagcggt
     3001 tactctatgg ggtatgatag acggtgtgct tattttagaa gtaaagaaaa gtaccttatt
     3061 tacaggattt atgatagatg cattaattgg acaactgatg gatatggtat tgctgggtgt
     3121 taagaagaaa taaaaaagtt attgaaaaaa tatatacata aaataaaaaa gcaaaaattt
     3181 gttatttaac aaattttaaa aaattataat taacttttta tcaggaggat ttctttatga
     3241 agctttcaaa acttttgatt gcgattgcgc tggttgttgg actggcagta cctacatttg
     3301 ctgcagacag cattgcaaag cttacgtttg gggacgatca gtatttagaa cttcactatt
     3361 tattgcaggt acaggcttat tc
//
LOCUS       DAOSZP010000205         3371 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_22327_length_3371_cov_3.662847, whole genome shotgun sequence.
ACCESSION   DAOSZP010000205 DAOSZP010000000
VERSION     DAOSZP010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3371
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22327_length_3371_cov_3.662847"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..454)
                     /locus_tag="PK348_05470"
     CDS             complement(<1..454)
                     /locus_tag="PK348_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HXK65695.1"
                     /translation="MQEITGTSNKVIEVDLTTQSHREIPISEKDLELYLGGKGLGLKL
                     FYDRVPAGIDPLSEENEFIVMTGVYLGTGAPCAGRFAAVTKSPLTGLMGTGSCGGPFG
                     MALKTSGWDGLIIKGKAKSPVYLVISSKGVEFKDAKKFWGKDTQTVQKA"
     gene            723..1211
                     /locus_tag="PK348_05475"
     CDS             723..1211
                     /locus_tag="PK348_05475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65696.1"
                     /translation="MHFETTTCISLQHIAILEEFAHKLGIPLRTIIVYMIMYAAKKEK
                     KQYRAFKRIAYRKRNADNPWKRVHLVLYQSEYEFFLDVKKLWKMSLAHVIEFCVDNIL
                     IEFFEYFKVRLAKINSDNYPENLPSYYENRSYTFDFYCENGIHCLKFYWGPPPEALYK
                     AI"
     gene            complement(1369..2277)
                     /locus_tag="PK348_05480"
     CDS             complement(1369..2277)
                     /locus_tag="PK348_05480"
                     /inference="COORDINATES: protein motif:HMM:NF021554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2279 domain-containing protein"
                     /protein_id="HXK65697.1"
                     /translation="MKSNNLLKPLTLIFTMILLFPIVLYGQEVPENTAPVQNESNWPT
                     IPTLAMFVAVPTITILYGMETWDWGEQSDWRWAHEGFFGQNTDSGGADKIGHGWAHYL
                     AFRIFHNYYDWSENGRNTKWIYSIATATFLGVGIEVGDAFTGKYGFSYEDVVADMLGI
                     GLGILLEYSPTLDSLIGFSWQYWPTESYFDRWGKNPLHMSSDYSGAKYVFNFRLAGLQ
                     NLGIKIPNFMRYIQLDIGYFTRNYVRWDETTADPYRSMYYGISLNFMEVVKDFFENPD
                     SKASRILQQPFKYYHIPAGYFVEDKI"
     gene            2544..2927
                     /gene="queF"
                     /locus_tag="PK348_05485"
     CDS             2544..2927
                     /gene="queF"
                     /locus_tag="PK348_05485"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR03139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HXK65698.1"
                     /translation="MTDYSKAKDALIEKVEGYVFDTIPARGHCVVVQWEYPEFQSLCP
                     VSGRHDQGTLLLVYEPKHLLLESKSVRDYLSAWRNKSIWQEYVTDVIAQDLYNAIKPK
                     WLSVEIQWAPRGGIFATTTSVKGKK"
     gene            2940..>3371
                     /locus_tag="PK348_05490"
     CDS             2940..>3371
                     /locus_tag="PK348_05490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65699.1"
                     /translation="MIAEYIAGVDIGGTKIIATIADATGIKARVYQHTQLQGDILTIP
                     KQVYYLIQKTAEYAGIHYEEVKAVGISTCSPFEKKGNYKEIVAPNLCGGLTGNDRLPN
                     SWHSIPLEEFFNGLYSHVVVENDCVAAVVAEKLFGAGKLYDN"
BASE COUNT         1116 a          608 c          625 g         1022 t
ORIGIN      
        1 gggctttttg aacagtctgc gtgtctttgc cccagaattt cttagcgtcc ttgaattcaa
       61 ctccctttga actgataact aaatacaccg gtgatttggc tttgcccttt ataatgagcc
      121 catcccaacc ggaagttttg agtgccatac caaatggccc accacaggag ccggttccca
      181 taagccctgt caatggtgat ttggtaactg ctgcaaaacg tcctgcacag ggtgctccgg
      241 tacctaaata cacaccggtc ataacaataa attcattttc ttctgataaa ggatcaatcc
      301 cagccggcac tctgtcatag aaaagtttta agcccaatcc cttaccacct aaatagagtt
      361 ccaggtcttt ttcagagata ggaatctcac ggtgggattg tgtggttaaa tcaacttcaa
      421 tgactttgtt acttgttcca gtgatttcct gcatatagta tctccttaaa acgttaattt
      481 ttttgatgta ctgattggcg ttaaaacaaa aacgattaat tgtattatta caataaaatt
      541 gtatatgagt aataatagaa tactgtaaaa ttatatgtca atcacgatat tgcaaagaat
      601 ataaaaaata agtacaatgt tataaattaa aacttatgat tgacaaaaaa aataaattat
      661 tattttaaat gggcaaacca ttaaaataat tccaaaacac ataaaatctt tgggaggaaa
      721 ttatgcattt tgaaacgact acctgtattt ctcttcagca tattgcaatc cttgaagaat
      781 ttgcacataa attaggcatt cctcttcgta ctattattgt ttatatgatt atgtatgctg
      841 ctaagaaaga aaaaaagcaa tacagagcat ttaaacgcat agcctaccgc aaacgaaatg
      901 ctgataatcc ctggaaacgg gtacatctgg tgttgtacca atctgagtat gagtttttcc
      961 tggatgtgaa gaagctgtgg aagatgtcgc tagcgcatgt tattgagttt tgtgtggata
     1021 atatactgat tgaatttttt gaatatttca aggtacgatt ggcaaagata aattccgata
     1081 actatcctga gaatttgcca agttactacg aaaacaggtc atacaccttt gatttttact
     1141 gtgaaaatgg tatccattgc ctaaaattct actgggggcc accgccagaa gccttgtata
     1201 aagctatata agcacaaaat tcataaaatc aggcggaaat ttttttctat atcattgcat
     1261 tatatgcaaa tgattattag ccatattgaa gatgaaaaaa ccgctttatg gtaaactgtc
     1321 tggcataaag cggttttata tgttatcttt taatgtgtta taatttaact atatcttgtc
     1381 ttcaacaaaa tatcctgcag gtatgtggta atatttaaac ggttgttgta agatgcggct
     1441 tgctttagaa tcaggatttt caaagaaatc ttttactact tccatgaaat tcagagaaat
     1501 accgtagtac atactacgat aaggatctgc agtggtttca tcccatcgaa cgtagttacg
     1561 tgtaaaatag ccaatatcca gttgtatata acgcatgaag tttggtattt ttatccctaa
     1621 gttttgcaga cctgctaacc tgaaattaaa aacatatttt gctccactgt aatcactgct
     1681 catatgcagt ggatttttac cccagcggtc aaaataactt tcagtgggcc agtattgcca
     1741 gctaaaacca atgagtgagt ccagtgttgg ggaatattcc agaagaatac ccaatcctat
     1801 tccaagcata tccgccacaa catcttcata ggaaaaacca tacttgcctg taaatgcatc
     1861 gcccacttca ataccaactc caagaaaagt agcggttgca atggaataaa tccatttagt
     1921 gtttctgcca ttttctgacc agtcatagta attgtggaaa attctgaacg ctaaataatg
     1981 agcccagccg tgaccaattt tgtcagcacc accggaatca gtattttgtc caaaaaaacc
     2041 ttcatgagcc catcgccaat cactttgttc accccagtcc catgtttcca tcccatataa
     2101 tattgtaatt gtaggtactg ccacaaacat tgccagtgtt ggaattgttg gccagttgct
     2161 ttcattctga acaggtgcag tattttcagg tacttcctgc ccataaagaa caatggggaa
     2221 tagtagtatc attgtaaaaa ttagggtgag tggctttaac aagttgttgc ttttcataat
     2281 aatgccctca acatacattg atctttacat tctgcaggtg ctatgggtat tcaaaaacaa
     2341 ttacataaag cagaatatta aaaagcacag aaaagtttaa aatcagatat gtcaatgaaa
     2401 tttaataaaa taaatataaa aaaataaatg tataaacaaa ataacagtac agtaataatg
     2461 agtgcataaa cgatgaattt ttgttgtaat ttttacttat agctatatta catataagta
     2521 gactttcatc gaaaggaatg accatgacag attattcaaa agctaaagat gcactaattg
     2581 aaaaagttga agggtatgtc tttgacacaa tacctgcacg cggccattgt gtggtagttc
     2641 agtgggaata tcctgaattt cagagtttgt gcccggtgtc tggcaggcac gatcagggta
     2701 ccttactact tgtgtatgag ccaaaacatt tacttttaga aagcaaatca gtccgtgatt
     2761 atttgagtgc atggcgaaat aaatcaatat ggcaggaata cgttactgac gtcattgcac
     2821 aggacctgta caatgcaatc aagccaaaat ggctttcagt ggagatccag tgggcaccac
     2881 gtggcggaat atttgctaca actacatcgg taaaaggcaa aaagtaaata aaggtcacta
     2941 tgatagctga atatatagca ggtgtcgata ttggtgggac aaaaattatt gcaactattg
     3001 ctgatgccac aggtataaaa gcacgggttt accagcatac acaattgcag ggggatatcc
     3061 tgacaattcc caaacaggtt tattatttga ttcaaaaaac agcagagtat gctggcatac
     3121 actatgaaga agtaaaagca gttggtataa gcacctgcag cccttttgaa aaaaaaggaa
     3181 actataaaga gatagttgct ccaaatcttt gcggtggatt aaccggcaat gatcgtttac
     3241 ccaattcatg gcactctata ccacttgaag aatttttcaa tgggttatat tcgcatgttg
     3301 ttgtagaaaa tgactgtgtg gcagcagttg ttgcggaaaa actttttggt gcgggcaaat
     3361 tatatgataa t
//
LOCUS       DAOSZP010000206         3370 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_22337_length_3370_cov_3.012971, whole genome shotgun sequence.
ACCESSION   DAOSZP010000206 DAOSZP010000000
VERSION     DAOSZP010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3370
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22337_length_3370_cov_3.012971"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            58..852
                     /gene="flgG"
                     /locus_tag="PK348_05495"
     CDS             58..852
                     /gene="flgG"
                     /locus_tag="PK348_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004494936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HXK65700.1"
                     /translation="MMRSLWTAASGMIGQQFNIDTISNNLSNVNTTGFKKLRAEFEDL
                     IYQTVLMAGTPATEISEVPTGIQVGHGVKVAATQKLFDQGSLQATGNKLDIALEGEGF
                     FKVQLYDGTIAYTRDGSFKVDSNRQIVTSNGYLLEPPIVLPENFIMETLSISQDGRIT
                     VKVVGDDEPIEVGQLEIARFVNPAGLQNIGGNLYKTTPASGEEIAGPPGIDGMARTHQ
                     GFLEMSNVKVVEEMVNMIVAQRAYEINSKAIQTSDSMLGTAIGLKR"
     gene            852..1379
                     /locus_tag="PK348_05500"
     CDS             852..1379
                     /locus_tag="PK348_05500"
                     /inference="COORDINATES: protein motif:HMM:NF024544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella basal body P-ring formation protein
                     FlgA"
                     /protein_id="HXK65701.1"
                     /translation="MNRIIVATIIISALFQSLLYADLAVYLKSNTAPKNNLSISDIAQ
                     IDGDESELVKKITIPESVLDDGYITKNEVYKLLKDVVTGLLVIHGSAVKLVVPESIST
                     SDSSLQELSPIYNELVIVKGDLVTVKLIKKSIVIELIGKALQSGKTGDEIKISLQSGK
                     TVTGKIVNNGVECYL"
     gene            1376..2002
                     /locus_tag="PK348_05505"
     CDS             1376..2002
                     /locus_tag="PK348_05505"
                     /inference="COORDINATES: protein motif:HMM:NF014195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HXK65702.1"
                     /translation="MKKFLLAIALIMISAVYADTIWKDKNIYTAQDTIKVGDIFTVAI
                     TDISQMRFTLTLANNDAFTVTSTPDGNITPFLPQVNANKKATKNNSTNLQHRGNITLH
                     IAATVQQRLENGNFRIAGTKTFSFSGVTIVFNVSGIVNPASIKGGTINSSDIANLVLQ
                     IQGVKEGVRITREPLKEGETASPALTEQEKQQIIIQYLQRILGELTQQ"
     gene            1999..2991
                     /locus_tag="PK348_05510"
     CDS             1999..2991
                     /locus_tag="PK348_05510"
                     /inference="COORDINATES: protein motif:HMM:NF014206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HXK65703.1"
                     /translation="MMNTVYKIIICIMTVTFFTVNLCAEVPVKIKDLVAIDGLKENQV
                     YGYGLVIGLQGTGDSKVKLTQSSLSNVLKNLGLNDENINSKNIAAVLVTATLPPYVRI
                     GDRIDVTVSSIGDAKSLENGVLLQSALKGADDTTYVVAQGVVLIAGDKRKGATIGRIV
                     SGGIVEKEILPDIVKDGNIKFSLLNFDFSVAKEIKTAIEKKYPESKPIIDKGYIQCTL
                     PQNVTVTDFIADIENIEIVPVYSARVVINERSGTIVMGGDIRVSQAVISKQGVTITIE
                     ETRKKGSMYLLKDAATVKDLVDSLNYIGMSTQDIIVILKAMKQAGCLHAELIVQ"
     gene            2999..>3370
                     /locus_tag="PK348_05515"
     CDS             2999..>3370
                     /locus_tag="PK348_05515"
                     /inference="COORDINATES: protein motif:HMM:NF021638.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod-binding protein"
                     /protein_id="HXK65704.1"
                     /translation="MDTTIKNIIDSQKTVQSINKQKDIEQKLHNTSTDFKSMLNNAIA
                     KNNDKPVDKKLMDVCIEMESLFVYQMLKEMRKTVHKENDLLYGGMAQEIFEDMLYNEY
                     ALQMSKTANFGLAKTLYEQLSQ"
BASE COUNT         1187 a          507 c          684 g          992 t
ORIGIN      
        1 ataaatatgc cgaaaagtac ataaaagtac aatattttac tattacgagg tgtcgatatg
       61 atgcgttcat tgtggacagc tgcatcaggg atgataggac agcaatttaa tatagatacg
      121 atatctaata atttatcaaa cgttaataca acgggtttta aaaaactaag ggctgaattt
      181 gaagatttaa tctatcagac cgtccttatg gccggtacac ctgctaccga gataagtgaa
      241 gtaccaacag gtatacaggt tgggcatggt gtaaaggtgg cagcaactca gaagcttttt
      301 gatcagggaa gtttacaggc aacggggaac aaacttgata ttgccctgga aggtgaaggt
      361 tttttcaaag ttcagcttta tgatggtact attgcatata cacgtgatgg atcatttaaa
      421 gttgattcaa acagacagat agttacttca aacggatatt tactggaacc acccattgtg
      481 ttaccggaaa attttattat ggaaacactt tctatcagtc aggatggcag aattaccgtt
      541 aaagttgttg gcgatgatga acctattgaa gttggtcagt tagaaatagc ccgctttgtt
      601 aaccctgctg gcttacagaa tattggtggt aatctatata aaacaacacc tgcatcaggt
      661 gaagaaattg cagggcctcc gggaattgac ggtatggcac gcacacatca gggattttta
      721 gaaatgtcta acgtaaaagt tgtcgaggaa atggtaaata tgattgtagc tcagcgtgcg
      781 tatgaaatta attcaaaagc aatacagacg tctgattcaa tgttaggtac tgctattgga
      841 ttgaagcgat aatgaataga ataattgtag caactataat tatttcagca ttgttccaat
      901 cattgctgta tgcagatctg gcagtatatt taaagtcaaa tactgcacca aaaaataatc
      961 tttctatttc tgatatagct cagattgatg gcgatgaatc tgaattggtg aagaaaataa
     1021 ctatacctga atctgttctt gacgatggat acatcactaa aaacgaagtt tataaattat
     1081 tgaaagatgt tgttacggga cttctggtta tccacggtag tgcagtaaaa ctggttgtac
     1141 cagagagtat atctacgtct gatagctcat tacaggaact atcgcccata tataatgaat
     1201 tggtaattgt taagggtgat cttgttactg tgaagttaat taaaaaatct attgtaatag
     1261 agttgattgg caaagcgctt caaagtggca aaacaggaga tgaaattaag atttcattac
     1321 aaagcggcaa aacagttaca ggcaaaattg tcaataatgg tgttgagtgt tatctatgaa
     1381 aaaattttta ctggcgatag ctcttattat gatatctgct gtttatgcag atactatctg
     1441 gaaagataaa aatatctata cagcgcagga tacaatcaaa gttggtgata tttttactgt
     1501 agctataacc gatatttcgc agatgcgttt tactctgact cttgccaata atgatgcgtt
     1561 tactgtaact tcaactcccg atgggaatat tacaccattt cttcctcagg ttaatgcaaa
     1621 taaaaaagct acaaaaaata acagcacaaa tcttcagcac cgtggaaaca ttacacttca
     1681 tattgcagca actgtacagc agcggcttga aaatggaaat ttcaggattg caggcacaaa
     1741 aacattttca ttcagtggcg taactatagt gtttaatgtt agtggtattg ttaatcctgc
     1801 atcaataaaa ggcggtacta tcaattcaag tgatatagca aatttagttt tacagataca
     1861 gggtgtaaag gaaggcgtaa gaattacacg agaaccactt aaagaaggtg aaaccgcttc
     1921 accagcattg accgaacagg aaaagcagca gatcattatc caatatttgc aaagaatttt
     1981 gggtgagtta acacagcaat gatgaatact gtatataaaa taattatctg tataatgaca
     2041 gttacatttt ttactgtaaa tctatgtgca gaggttcctg taaaaatcaa agatctggtt
     2101 gccatagatg gattaaaaga aaaccaggta tatggatatg gacttgttat tggtttacag
     2161 ggtactggtg atagcaaagt taaattaaca cagagttctc tttccaatgt attaaaaaac
     2221 ttagggctta atgacgaaaa tattaattct aaaaatatag cagcagtcct ggtaactgca
     2281 actctgccac cgtatgtaag aattggtgac aggattgacg ttactgtttc atccataggt
     2341 gatgcaaaat cactggaaaa tggtgtttta ctacaatcag cattaaaagg tgctgatgat
     2401 actacatatg ttgtggcaca gggtgttgtt ttgattgctg gtgacaagcg taaaggggca
     2461 accataggca ggatagtttc tggtggtata gttgaaaaag aaattctgcc tgatatagtt
     2521 aaagacggca atattaagtt ttcacttctt aattttgatt tttcggtagc aaaagaaata
     2581 aaaacagcta ttgaaaaaaa gtatcctgaa tcaaagccca taatagataa gggatatatt
     2641 caatgtactc ttccacaaaa tgttactgtt actgatttta tagcagatat agaaaacatt
     2701 gagatagtac ctgtatatag tgcacgagtg gtaattaatg aacgaagtgg taccattgtg
     2761 atgggtggtg atattcgtgt ttctcaggca gtaataagca agcaaggggt aacaataact
     2821 atcgaagaaa cacgtaaaaa aggtagcatg tatttattaa aagatgctgc aactgtcaaa
     2881 gatttagttg attcattgaa ttatattggt atgagtactc aggatattat cgtaatttta
     2941 aaggcaatga aacaggcagg atgtctgcat gcagagttga tagttcagtg aggtttgtat
     3001 ggatacaaca attaaaaata ttatcgacag ccagaaaacc gtacagtcta ttaataaaca
     3061 aaaagatata gaacaaaaac tacacaatac ctctacagat ttcaaatcca tgcttaataa
     3121 tgcaatagct aaaaacaatg ataaacctgt tgataaaaag cttatggacg tttgcattga
     3181 aatggaatca ctatttgtat atcagatgct caaagaaatg cgcaaaacag ttcacaagga
     3241 aaatgattta ttatatggtg gaatggcaca ggaaatattt gaagatatgc tgtacaatga
     3301 atatgcactc cagatgtcaa aaacagcaaa ttttggactt gctaaaacac tctacgaaca
     3361 gttaagccag
//
LOCUS       DAOSZP010000207         3366 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_22374_length_3366_cov_2.813651, whole genome shotgun sequence.
ACCESSION   DAOSZP010000207 DAOSZP010000000
VERSION     DAOSZP010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3366
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22374_length_3366_cov_2.813651"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..954
                     /gene="metG"
                     /locus_tag="PK348_05520"
     CDS             <1..954
                     /gene="metG"
                     /locus_tag="PK348_05520"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF039287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase subunit beta"
                     /protein_id="HXK65705.1"
                     /translation="YFFLREMTFGLDAKFSEEAIVNRINYDLANDLGNLINRTFNMVN
                     KYCDAKVPSFHDDSPAGKTDMQKALDDAMNHYFDYIDAFQFNLALERLWDFIRYCNKY
                     IDEHKPWQLAKEGNTRQLQSLLRNLLEGIYAIAYLLAPVCITKSPVIINALKAPVQYP
                     VEQLVKLSALTEGSIIDNPGILYPRLEKENDRAPQKTKKEEKPMEELEGLIDIKDFMK
                     VELRIAKVLQAQAVEGSSKLIELQIDTGKDTRTIVAGLAPHYTPEYLVGKKIIVVANL
                     KPATLFKRTSHGMLLAAKLSKDDKPILIEVNDTVPVGAVLS"
     gene            971..1465
                     /locus_tag="PK348_05525"
     CDS             971..1465
                     /locus_tag="PK348_05525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YfcE family phosphodiesterase"
                     /protein_id="HXK65706.1"
                     /translation="MKIGLISDTHNDIEMVQKAIAIFKERNISVIIHAGDLTSSKIIS
                     LFKGFDGRFVLGNCDIDVEEINTTAAECGFGCVEHSCQFTLDGKHFMVFHGNNVPMFR
                     EAVNSGTYDYIIKGHTHIFENYVSGKTRIINPGSLYRGEEHTVAILDTETDKVEKIRI
                     DIEE"
     gene            complement(1466..2335)
                     /locus_tag="PK348_05530"
     CDS             complement(1466..2335)
                     /locus_tag="PK348_05530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HXK65707.1"
                     /translation="MAKKVLGKGLGAIITTSPTPVSDFEKVLLQDKERIVDIPINAIM
                     PNPDQPRVHFDAAEIAGLAQSIQHVGLLQPIIVRKQGEEYFIVAGERRWRACKLLGKP
                     TIKAIVMNTTEEQNLTLALIENIQRTNLDPIEEAKAYKMLATRFKLKQSEIADRVGKD
                     RATVANIMRLLNLPEHMQEALQDGRINVGHAKLLLGVSGDEQEALFDRIIHQGLSVRD
                     VEKAIAEVPKTSGKKKKKAKDAHIRKLEEELISYLGTRVEIRHSAGKGRIEISFYSLD
                     DFERIVSLIKGDR"
     gene            complement(2364..3128)
                     /locus_tag="PK348_05535"
     CDS             complement(2364..3128)
                     /locus_tag="PK348_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXK65708.1"
                     /translation="MGKIISISNQKGGVGKTTTAVNLAAFLAEKGKQVLVVDIDPQAN
                     AGFGLGVNVEEMETTVYEVLLGTIPVQEAIFRTTIENLYIVPSNMHLAGAQVELMDME
                     DKEYLLKRALEKVRDTFDFILIDCPPSLGILTLNSLVASDSVIIPLQCEYYALEGLSQ
                     LLKIISMVQDTLNPKLEIEGVLLTMYDPRTNLSQQVVSDVKQFFKNKVFSAIIPRNVR
                     LSEAPSFGKPINLYDRTSSGSMSYEQLAHELIQNVR"
BASE COUNT          992 a          714 c          649 g         1011 t
ORIGIN      
        1 tatttctttt tacgggagat gacatttggg cttgatgcta aattttcaga agaggcgata
       61 gttaatcgta ttaactatga ccttgcaaat gatttaggta atttaataaa ccgaaccttt
      121 aatatggtga acaagtactg cgatgctaaa gtaccatcat ttcatgacga cagtcccgca
      181 ggaaaaacag acatgcagaa ggcacttgat gatgccatga accactattt tgattatatt
      241 gatgccttcc agtttaatct ggcgcttgag cgcctgtggg attttatacg ctactgcaat
      301 aaatacattg atgaacacaa accatggcag ttagccaaag agggcaatac caggcagctt
      361 cagtcacttc tgagaaacct gttagaagga atatatgcaa ttgcatacct ccttgcaccg
      421 gtgtgcatca ccaaatcgcc tgtcatcatt aatgcactta aagctcccgt acagtatccg
      481 gttgaacaac ttgtaaagct atcagcactt actgaaggaa gcataattga taacccggga
      541 attctgtacc cgcgattaga aaaagaaaat gacagagcac cacaaaaaac taaaaaagag
      601 gaaaaaccaa tggaagaatt ggaaggcctt attgacatca aggattttat gaaagttgaa
      661 ctccgtattg caaaggtatt gcaggcacag gcggtagaag gatcaagtaa actcattgag
      721 ttgcagatag ataccggcaa ggatactcgc accattgttg cggggcttgc gcctcattat
      781 actcctgaat atcttgttgg gaaaaagatc attgttgtgg caaatcttaa accagccaca
      841 ttgtttaaac gaacatcaca cgggatgctt ctggcagcaa aattgtcaaa agatgacaaa
      901 cccattctga ttgaagttaa tgatacggtg ccggttggtg cagtactcag ttaatacacc
      961 atgaggcttt atgaagattg gattaatatc cgatacacac aatgatatag aaatggttca
     1021 gaaggcaatt gctatattca aagagcgcaa tatatccgtt attatccatg ctggtgacct
     1081 tacttcatca aaaattattt ctctgtttaa aggctttgat ggcagatttg tgctgggtaa
     1141 ctgcgatatt gatgttgaag aaataaatac aaccgcagca gaatgcggat ttggatgcgt
     1201 tgaacattca tgtcagttca cgcttgatgg caagcatttc atggtatttc atggcaacaa
     1261 tgttcccatg ttcagggaag ctgttaacag tggtacatac gattacatca tcaaaggaca
     1321 tacccatatc tttgaaaatt atgtcagcgg aaaaacacgt attatcaatc cagggtcact
     1381 ctaccgcggt gaggaacata ctgttgccat tcttgatact gaaactgata aagtggaaaa
     1441 aattcgtata gatatagaag aataactacc tgtcaccttt tataagactt acaatgcgtt
     1501 caaaatcatc aagcgaataa aaacttattt caatacgacc cttccccgct gaatgccgta
     1561 tttcaacacg agtacctaaa tatgagataa gctcttcttc cagtttccgt atatgtgcat
     1621 cctttgcttt ctttttcttt ttgccagaag tttttggcac ctcggctatt gctttttcta
     1681 cgtcacgcac tgaaagcccc tgatggatta tcctgtcaaa caatgcctcc tgctcatcac
     1741 cagaaacacc taaaagcagt tttgcatgcc caacattaat cctcccatcc tgcaatgctt
     1801 cctgcatgtg ctccggaaga ttaagcaagc gcatgatatt ggcaaccgtt gccctatctt
     1861 tgcccacacg atcagctatt tctgattgct tgagtttgaa gcgtgttgca agcattttat
     1921 atgcttttgc ttcttctatg ggatctaagt tggtgcgttg aatgttctct atgagggcca
     1981 gtgtcagatt ctgctcttcg gttgtgttca tgacaatggc tttgatggtt ggctttccca
     2041 ataacttgca tgctcgccac ctgcgttcac cggctacaat aaaatattcc tcaccctgct
     2101 tacgcacaat aattggctgc agcaacccca catgctgaat ggattgtgca agccccgcta
     2161 tctctgctgc atcaaaatgg acacgtggct gatcaggatt tggcattatt gcattgatag
     2221 gaatatctac aatacgttct ttatcctgta gcagaacctt ttcaaaatca ctcaccggtg
     2281 ttggtgacgt ggtgatgatt gctcccaatc ctttacctaa aacttttttt gccattatct
     2341 gctcctgtca ggtatcctgt gagctatcgc acattttgaa ttagttcatg tgccagttgc
     2401 tcatagctca tactgccact ggaagtcctg tcataaagat tgatgggctt gccaaacgac
     2461 ggtgcttcag aaagccgcac attgcgtggg attattgctg aaaacacttt attcttaaaa
     2521 aattgtttta catcagaaac cacctgctgc gaaagattgg ttcgcgggtc atacatggtc
     2581 agtagcaccc cttcaatttc aagctttgga ttgagtgtat cctgaaccat ggaaataatt
     2641 ttcagaagct ggcttaaacc ctcaagtgca tagtattcac actgcagtgg aataataacc
     2701 gaatctgatg caaccaggct atttaacgtg agtattccta atgatggtgg acagtcgata
     2761 agtataaaat caaatgtatc gcgtaccttt tccagcgccc ttttcaataa atactcttta
     2821 tcctccatat ccatgagttc aacttgagcc ccggctaaat gcatattgga tggaactata
     2881 taaagatttt cgatagttgt cctgaagatt gcttcctgta cgggaatggt acccagcaat
     2941 acctcataca ctgttgtttc catttcctca acattgacac ctaagccaaa tcctgcgttt
     3001 gcctggggat caatatctac aaccagaacc tgttttccct tttcagctaa aaatgccgct
     3061 aaattgacag cggttgtggt tttaccaacc cctcctttct ggttggaaat ggatataatt
     3121 ttacccattg atagctccct ttgaaaatat tcccaattta tacagactaa agctttaagc
     3181 aatcactata tatggcaaat cgaaatagtg atacatatgt agtacacata tactgctatg
     3241 atatgtatgt taccgtaaaa tgtatacata tgtatgaatc gtataccttc aattgcaagc
     3301 tttttttact ttatacttac accacaggtt gtggggaaat ataaatacct atttattttt
     3361 gcctat
//
LOCUS       DAOSZP010000208         3358 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_22437_length_3358_cov_3.369361, whole genome shotgun sequence.
ACCESSION   DAOSZP010000208 DAOSZP010000000
VERSION     DAOSZP010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3358
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22437_length_3358_cov_3.369361"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..500
                     /locus_tag="PK348_05540"
     CDS             <1..500
                     /locus_tag="PK348_05540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65709.1"
                     /translation="NPIAIAGADFSFPKCVTYARSTEYHYRYQLMHNRYTPLETKNMD
                     YIIHASKRTRTTTFSRKNFIQYHDAVDAILPKDTSLFHINPVLPLAHAKSIAIQDFLS
                     LPQVSHYTKKMTARLLQPLSHSINLNEIYTILNNETMFNTIVNSSQVHNQKSIHRIHY
                     LLHKE"
     gene            505..1095
                     /gene="coaE"
                     /locus_tag="PK348_05545"
     CDS             505..1095
                     /gene="coaE"
                     /locus_tag="PK348_05545"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HXK65710.1"
                     /translation="MRIGVTGIYASGKGTVCSMFEKLGAIVIDTDIIAREIVNPPSPV
                     LDTLVHTFGHDILNTDGTLNRRYLAQKVFNDKMLVEKLNSITHPAILQEVMQRSHDSS
                     KIYMINTPLLFETEYHKHMDKNIVVVANTEQVLKRGVIRDGITEDEIKARLNHQISLK
                     EKMKLADYIIDNSGSLENTKKQVEELWKTLILMKGQ"
     gene            1062..1832
                     /locus_tag="PK348_05550"
     CDS             1062..1832
                     /locus_tag="PK348_05550"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HXK65711.1"
                     /translation="MENFDFNERPVAREKNVFLLHLDVPRIILISAVLIGIIVTTFLL
                     GMNFSENKVVSHNTLVPPEIPGDTIAQLDKGIDLGNQVQNPDLPLMDNASNKLDIAPK
                     PDEPSKLDIIPKKDMSDTSIAKLEDTETTQVIHENVKKVTPVKNISKKNTKAKSNVKK
                     NKVVEVAKTDESHTKKVSQRSWSIQIASYNTLSKAQKEVKSLQTMKYDAYVDKADVSG
                     KTYYRVKIGPILKEDKAINMLQEIQSIDKYKESYIVKE"
     gene            complement(1897..>3358)
                     /locus_tag="PK348_05555"
     CDS             complement(1897..>3358)
                     /locus_tag="PK348_05555"
                     /inference="COORDINATES: protein motif:HMM:NF012731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HXK65712.1"
                     /translation="LQTSFGINLLALVNNQPKLLDLKGLITHYISHRKTVVTRRTQYE
                     LARAKERAHILEGLKIALDNIDEVIAIIRGSKTVEEANERLIKRFKLSEIQSKAILDM
                     RLQRLTSLEVKKVIDELRELLKLIDELTAILKSEKRIFAIIKDELIAIKEKYHDTRRT
                     EIIYEDTSVSFDAEDLIAEEDMVITITNDGFIRRLSADTFKKQRRGGKGVIGLSSKKE
                     DFITMMTIASTHDTLLLLSNKGKIFGMKVYEIPVASKNTRGKSLKGIINLASGEMITA
                     ICSVNDFSENFLCMVTRNGVMKKTALEEFSNVKKGGIIAINLHKDDELVDVKVVKSQD
                     DVVIATRNGLLLRTNIAKMRAMGRNSSGIIGMRVASGDQIIAMDVVKPGSDLCVVTSR
                     GYGKKTQYSHFATKGRGGKGMAYLKISEKNGYAVGVRSVHPEDEVIIASKKGMTIRLK
                     AKEISYQGRTAAGVLLLQLDKDDEVTDFAVLYEEKE"
BASE COUNT         1092 a          694 c          539 g         1033 t
ORIGIN      
        1 gtaatccaat agccattgca ggtgcagatt tttcatttcc caagtgtgtt acatacgccc
       61 gatcaacaga ataccattac cgctatcaac ttatgcataa ccgttataca ccacttgaaa
      121 caaaaaacat ggactacata atccatgcgt caaaacgtac aagaaccaca accttttcaa
      181 ggaaaaactt tatccagtat catgatgcag ttgatgctat ccttcccaaa gacacatcac
      241 tatttcatat caatcccgta ttaccacttg cccacgcaaa gtcaatagca atccaagact
      301 tcctttcact gccacaggtc agccactata caaaaaagat gacagcacgt ttgcttcaac
      361 ctctatcaca ttcaataaat ctcaatgaaa tatatactat tcttaataat gaaacaatgt
      421 ttaatactat tgtaaactcc tcccaagtac ataatcaaaa aagcattcat agaattcatt
      481 atttattaca caaggagtaa atctatgagg attggcgtta caggcattta tgcatctggc
      541 aagggaacag tatgttccat gtttgaaaag ttaggggcga tagtaattga taccgacatc
      601 attgcccgtg aaatagttaa cccgccaagc ccggtgcttg atacactggt gcatacattt
      661 gggcatgata tactcaatac agatggaaca ctgaacagaa gatatttagc gcaaaaagta
      721 ttcaatgata aaatgcttgt tgagaagctt aattccataa cccatcccgc aatattgcag
      781 gaagttatgc aaagatccca tgatagtagc aaaatctata tgattaatac tccactgctt
      841 tttgaaacag aatatcacaa acatatggat aaaaatatcg tggttgttgc caatacagag
      901 caggtgctca agcgtggagt aatccgtgat ggcataactg aagatgaaat caaagcacga
      961 ttaaatcatc aaatttcact taaagaaaag atgaaactgg ccgattatat catagataat
     1021 tctggatcat tagaaaatac taaaaagcag gttgaagaat tatggaaaac tttgatttta
     1081 atgaaaggcc agtagccagg gaaaaaaatg tttttctcct tcatcttgat gttccccgca
     1141 ttattctgat cagtgctgta cttattggca taatagttac tacatttctt ttaggaatga
     1201 atttcagtga aaataaggtt gtttcccata atacattagt tcctcctgaa attccagggg
     1261 acacgatagc acagcttgat aaaggtattg atttgggtaa ccaagtgcaa aatcctgatc
     1321 tgcctttaat ggacaatgcc agtaataaac ttgatatagc ccctaaacct gatgaaccat
     1381 caaaactgga tattattccc aaaaaagata tgtcggacac ttctatagca aaactggaag
     1441 atactgaaac cacccaggtt atccatgaaa acgtgaaaaa agtaacacca gttaaaaata
     1501 tatcaaaaaa gaataccaaa gccaaaagca atgtcaaaaa gaataaagtt gttgaagtag
     1561 caaaaactga tgaatctcat accaagaaag tatcccagcg atcatggtca atacagatag
     1621 catcgtataa tactctttcc aaagcccaga aagaagtaaa gagtttacag acaatgaaat
     1681 acgatgcata tgttgataaa gcagatgtct ctggtaaaac atactatcgc gttaaaatag
     1741 gacctattct gaaagaagat aaagctataa atatgctgca ggaaattcag tcaattgata
     1801 agtacaaaga aagttacatt gtaaaagaat aatagcaaaa aacccgcttg tacatagcgg
     1861 gttttttata ctatgttttg tttttactta ttttttttat tccttttctt catagagaac
     1921 cgcaaaatca gttacttcat catccttatc caattgcaat aaaagaacac ctgctgcagt
     1981 tcttccctga tatgatattt cttttgcttt taatcgtatg gtcatccctt ttttagatgc
     2041 aatgataacc tcatcttcag gatgcactga ccgtacccca accgcatacc cattcttttc
     2101 acttatcttc aggtatgcca ttcctttccc gcctctacct tttgtggcaa aatgtgaata
     2161 ctgtgtcttc ttaccatacc ctcgagaggt taccacacaa aggtctgatc ctggtttaac
     2221 aacatccatg gcaataatct ggtcacctga tgcaacacgc ataccaataa tacccgagga
     2281 attcctgccc attgcccgca tctttgcaat atttgttctc agcagaaggc catttctggt
     2341 tgctatcacc acatcatcct gcgatttaac caccttaaca tcaacaagct catcatcctt
     2401 atgcagatta atagcaatga taccgccttt tttcacattg ctgaattctt ccagtgccgt
     2461 ctttttcata acaccattgc gagtgaccat acataaaaag ttttcactga aatcatttac
     2521 ggaacaaatt gcagttatca tttcgcccga agcaaggttt ataatgcctt ttaatgactt
     2581 accccgtgta ttttttgaag ctaccggtat ctcataaacc ttcatgccaa agattttacc
     2641 tttgttagat agcaataaca atgtatcatg tgttgaggcg atagtcatca tggtaataaa
     2701 atcttctttt ttagaagaaa gaccaatcac acctttaccg ccacgacgtt gctttttaaa
     2761 tgtatctgcc gatagtcgcc gtataaaccc gtcattggtg atggtgatga ccatatcctc
     2821 ttcagcaata aggtcttctg catcaaagct tactgaggta tcttcgtaga taatctcagt
     2881 tcttcttgta tcatggtatt tctctttgat ggcaataagt tcatctttaa ttattgcaaa
     2941 aattcttttt tcgcttttta atatagctgt cagctcatca atcaatttta ataattctct
     3001 aagttcatca atgacctttt ttacttccag gcttgtcaat cgctgcaacc gcatgtcaag
     3061 aattgccttt gattgaattt ctgatagctt aaaccgtttg atgagacgtt catttgcttc
     3121 ttcaacggtt tttgaccctc tgattattgc aatgacttca tcaatgttat caagtgctat
     3181 cttcaatcct tcaagtatat gggctctttc ttttgctctg gctaattcat actgtgtacg
     3241 gcgggtaaca acagtttttc tgtggcttat gtaatgtgtt atcaatcctt ttaaatccag
     3301 cagttttggc tgattattta ccagcgccag aagattaatg ccaaatgatg tctgaagt
//
LOCUS       DAOSZP010000209         3337 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_22620_length_3337_cov_3.103900, whole genome shotgun sequence.
ACCESSION   DAOSZP010000209 DAOSZP010000000
VERSION     DAOSZP010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3337
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22620_length_3337_cov_3.103900"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..677)
                     /locus_tag="PK348_05560"
     CDS             complement(<1..677)
                     /locus_tag="PK348_05560"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012675658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HXK65713.1"
                     /translation="MIVCIQHVPFETPGYILDWAIVHQKDVAIVTAYTNHDFPVVDSV
                     EMLVIMGGPMSVHDEREYPWLVTEKHFIEKVINKGKPVLGICLGAQLIADVLGARVYK
                     NHTKEIGWFEVQRVQSENPFSKLLPDTFMAFHWHGETFDIPAGAIHIASTDVCANQAF
                     VIDDRIVGLQFHLEITAQGVYDLVANCGAELKENGHIQSKDAIIAGISYCKHTHALMD
                     SLLEAFT"
     gene            complement(680..1000)
                     /locus_tag="PK348_05565"
     CDS             complement(680..1000)
                     /locus_tag="PK348_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXK65714.1"
                     /translation="MGNLFNDPQNFVNAIEYQSNAIVSKTIIQNKSGSLTLFAFGQGQ
                     SLSEHTAPYDAFVMVLDGQFIITIGGKENTVKAGEAIIMPANVPHALVAANNAKMFLV
                     MIGG"
     gene            complement(1021..2325)
                     /locus_tag="PK348_05570"
     CDS             complement(1021..2325)
                     /locus_tag="PK348_05570"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HXK65715.1"
                     /translation="MIQNKQKKVVIIGAGFGGLWVAKTLANTGFHVTIIDKNNYHTFL
                     PLLYQVSAAEVAPEQIASPVRNIVRKHNNIQFIRGAVSKIQYSTKTVLCLGQEIPFDY
                     LIVSAGSENNYFNIPGVARYAFPLKTLDDAMVLRNHILTCFERASFVNDEKQRKRLLT
                     VVIVGGGPTGVEFAGALAELIAGPLKKDFPELQHEEITVYLIEASDRLIGMYPEKLGD
                     YTCKQLQKKGVKVVHNAAVTNIDATGVQLNNGGIIPTETVVWTAGVKGELIANDLNVK
                     TMPNGRVIVDEYCRVPGYDDVFIIGDLACFMHNGKPLPMIAPVAMQQGRYVGKHLLQK
                     LKGKTPKPFRYRDKGGMVAIGRNKAIAQIAGLHVKGYIAWILWIFIHILYLIGFKNKI
                     FVMINWVYSYIFFEKSVRLILPRCCDDPHGDACLQRGRSCKG"
     gene            complement(2343..>3337)
                     /locus_tag="PK348_05575"
     CDS             complement(2343..>3337)
                     /locus_tag="PK348_05575"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HXK65716.1"
                     /translation="QLHIDLFRESIDAGKVDVPEGHPLYILYTEHSRILEFAQQLVES
                     ATRLFDTPDSATFARIQQLIGFFKESAVHYLREENVLFPVLEKYGVTQPPKIMWMEHD
                     NIRAIEKSIYETADALAKGITKELSHTLQTHVIRLSETLADHFYKENNILFPTSHKFF
                     NEDDWKLVKQEFDGIGYMSYSPVIHIEKGEGAGTRLASDTVEFETGNLTLEQLQYMLD
                     TMPVDFTFIDKDDVVRYFNNAPDRIFVRSKAVIGRTVQNCHPDKSIHVVNRLLDEMKQ
                     GKRNKAEFWITLNDRLIYITYYAVRKNGDYIGCLEVTQDITDIQKITGQKRLLD"
BASE COUNT          979 a          792 c          539 g         1027 t
ORIGIN      
        1 atggtaaaag cttcaagcag tgaatccatt aatgcatgcg tatgtttgca ataactaata
       61 cctgctataa tagcgtcttt actctgaata tggccatttt ccttaagctc agccccacaa
      121 tttgctacca gatcataaac accctgtgct gtaatctcca gatgaaattg cagccctact
      181 attctatcat caatgacaaa tgcctgattg gcacatacat cagtagatgc tatatgtatt
      241 gcacctgcag gtatatcaaa tgtttcacca tgccagtgaa atgccataaa tgtatcaggc
      301 aacagtttgc taaatggatt ttctgattgt actcgttgta cttcaaacca cccaatttct
      361 tttgtatgat ttttatacac tcgtgctccc agtacatcag caataagctg tgcaccaaga
      421 caaatcccta aaacaggttt gcctttattt attacttttt ctataaaatg cttttctgta
      481 acaagccatg ggtattcccg ttcatcgtga acactcatag gcccacccat aattacaagc
      541 atctcaactg aatcaacaac tgggaaatca tggtttgtat atgcagtaac tatcgccaca
      601 tctttttgat gcacaattgc ccaatccagt atatagcctg gcgtttcaaa tggtacatgc
      661 tgaatacata ctatcatgat tatcctccta tcataacaag aaacatcttt gcattatttg
      721 ctgcaacaag ggcatggggt acattagctg gcataatgat agcttcccct gctttcacag
      781 tattttcttt gccaccaatg gtaatgataa attgaccatc aagcaccatt acaaaagcat
      841 catacggtgc agtatgctcg ctcagacttt gtccctgccc aaatgcaaag agagttaaac
      901 taccgctttt attctgtatt atagttttgc tcacaattgc attgctttga tattcaattg
      961 cgttaacaaa attttgtggg tcattgaata gattacccat agtattcctc ccatacaatg
     1021 ttatccctta caactccttc cgcgttgcaa acacgcatcc ccgtgaggat catcacaaca
     1081 tcgtggcaaa ataagccgca cacttttttc aaagaagata taactgtata cccagttaat
     1141 cataacaaaa attttatttt taaaaccaat caaatacaaa atatgaataa agatccatag
     1201 tatccatgca atatatccct tcacatgcaa acctgcaatc tgagcaatag ctttattacg
     1261 tcctatagca accatcccac ctttatccct atacctgaaa ggtttgggtg ttttgccttt
     1321 aagcttttgc aataaatgtt tgcccacata tcttccctgc tgcatagcaa ctggtgcaat
     1381 catgggaagt ggtttgccat tatgcataaa acaggctaaa tcaccaataa taaacacatc
     1441 atcatatcca ggcacccggc aatattcatc aactatcacc cttccattgg gcattgtctt
     1501 aacattcaaa tcatttgcta taagttcacc cttaacacct gctgtccaca ccaccgtttc
     1561 agtgggtatt atgccaccat tgttcaattg tactcctgtt gcatcaatgt ttgttaccgc
     1621 tgcattgtgt acaaccttta cccctttctt ttgcagttgc ttgcaggtat aatcacctaa
     1681 tttttccgga tacattccaa taagtctatc gcttgcctca ataagataca cagttatttc
     1741 ttcatgttgc aattcaggaa aatctttttt caatggtcct gcaatcaact ccgcaagagc
     1801 accagcaaat tcaacacctg ttggacctcc accaacaatc accacagtaa gcaatcgctt
     1861 tcgctgtttt tcatcattca caaacgaagc ccgttcaaaa caggtaagga tatgatttcg
     1921 caaaaccatg gcatcatcca gtgttttcaa agggaacgca taacgagcaa cacccggaat
     1981 attaaaataa ttgttttcac tacctgctga tacaatcaga taatcaaacg gtatttcctg
     2041 acccaggcac agtacagtct ttgttgaata ctgaatctta ctaacagcac cacgtataaa
     2101 ctgtatatta ttgtgctttc taacaatatt gcggaccggt gatgctatct gttccggtgc
     2161 tacttccgca gcactcacct gatacaaaag tggcaaaaat gtgtgatagt tatttttatc
     2221 aatgattgtc acatgaaaac cagtatttgc taacgtcttt gcaacccaaa gcccgccaaa
     2281 gccagcacca atgataacga cttttttttg tttgttctgt atcaattgaa ttcctccctg
     2341 cattaatcca ataaccgctt ctgcccggtt attttctgaa tgtcggtaat atcctgagta
     2401 acttccaggc agccaatata atcaccattt ttacgcacgg catagtaggt gatataaatc
     2461 aaacggtcat ttaacgtaat ccagaactcg gccttattgc gcttaccctg cttcatttca
     2521 tccagcaggc ggttaaccac atgaatgctt ttatccggat ggcagttttg cacagtcctg
     2581 ccaataacag cctttgatcg cacaaaaata cggtcgggcg cattgttaaa gtagcgtacc
     2641 acatcatctt tgtcaataaa cgtaaaatct accggcatgg tatcaagcat gtattgaagt
     2701 tgttccaacg tcaggttccc tgtttcaaat tccacagtat cagatgccaa acgtgtgcct
     2761 gcaccctcac ccttttctat gtgaataacc ggactatatg acatgtaccc aataccgtca
     2821 aattcctgct taactaattt ccagtcatct tcattaaaaa acttatgcga cgttgggaac
     2881 agtatattgt tctctttgta aaagtggtca gcaagcgttt cactaagccg tatgacatgt
     2941 gtttgcaatg tgtgcgatag ttcctttgta atgccctttg ccagtgcatc agctgtttca
     3001 tatatactct tttcaattgc acggatgttg tcatgttcca tccacataat ctttggcggt
     3061 tgagtaactc catatttttc cagcaccggg aaaagcacat tttcttcacg aagatagtgc
     3121 accgctgatt ctttaaaaaa accaattaac tgttgaatgc gtgcaaacgt agcactgtct
     3181 ggtgtatcaa aaagtctggt tgcactttca actaattgct gcgcaaattc aaggatcctg
     3241 ctatgttcag tatacagaat atacaaagga tggccctcgg gcacatccac tttgcctgca
     3301 tcaattgatt ccctgaaaag atcgatatgc aactggc
//
LOCUS       DAOSZP010000210         3333 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_22651_length_3333_cov_3.528371, whole genome shotgun sequence.
ACCESSION   DAOSZP010000210 DAOSZP010000000
VERSION     DAOSZP010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3333
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22651_length_3333_cov_3.528371"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            229..1356
                     /locus_tag="PK348_05580"
     CDS             229..1356
                     /locus_tag="PK348_05580"
                     /inference="COORDINATES: protein motif:HMM:NF019403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HXK65717.1"
                     /translation="MNSKRIVHIDYLKAWAVIVMIEVHVVNCFLNPSFKTAWWFNIVN
                     FINGLVAPVFIFTAGFSFVFSVMKKREELISLSDLFFRRTGRILLIVLVGYLIHIPYF
                     SLKNIIIYASPAELKSFYSADVLQCIGSGLLVLLFLRMVIPSDVLYHSIITGIGLLIV
                     FITPYIWEIDFYQWFPLPIATYFNEMHGSLFPVFPWHGFLFLGAAYGVFQLHHNTNIH
                     ADKSLNTFAAISFIISIAGIISMHFLSQNPAYTIKTSAIFFITRYALVIVLLHCCQWY
                     LHIKNNQGGLFINIGKESLLVYWLHLQILYRHIFNGKSLERIVAQKFSFAECLTAFLL
                     LAALMFAVAIMWNTLKQHYPDISRKIFIGTILIGLIKFLSF"
     gene            complement(1359..2174)
                     /locus_tag="PK348_05585"
     CDS             complement(1359..2174)
                     /locus_tag="PK348_05585"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXK65718.1"
                     /translation="MTIQEQFIEALRLEEQGNVTEALQLYIQILQIDNSFRAALINLG
                     SLYYRMKQFNHALDCFLRALQLKEDYIVLFNIGSLYFRLGEYKKAIIILNKCSTSNPD
                     FYIALLVKGIAFSKINNYKAALGCFKEVIKKDPANKVALTAIILLYYEQKNYTDALRY
                     LQQYDRYHDSFRFREITSDIILQCAQYNDEKLISHSLQKKGFRQFNEYISTIPHTIFN
                     DSKGTIDTKIQQLEQEIQKEPTPQAFISLSLCHLFVGNNQTALQYLVKATKAV"
     gene            complement(2226..>3333)
                     /locus_tag="PK348_05590"
     CDS             complement(2226..>3333)
                     /locus_tag="PK348_05590"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HXK65719.1"
                     /translation="LSVIKTQELFYRVCMDDNGLTFFDETEPTPEGIEGLEKKIYDLR
                     NLLELGISLSSNLQFESLVESLLYSCIGQMFVEQVALLLQKDIDVNDFYIHMAKGYDS
                     DFEENVILQETSPLVGFLENNPYPIEYETLLELPDLADDLKLIAFLNPYIVVPLKSKN
                     SLMGVLLLGEKITGGGFSNSEKEFLHYVARFGAVAVENSRLYLMSTLDRMTRLYIHHY
                     FEIRLFEEMKRSQRYNAPLSLLMCDIDHFKRFNDTYGHLQGDSVLKEVAAIFLKDLRK
                     MDIPCRYGGEEFAVILPQTRLDQAGIVAQRLRKIVEQHPFKGQDSTLHVTISIGVTQY
                     DPARDTSIKEFVGRSDKALYKAKEMGRNKVALAK"
BASE COUNT          996 a          651 c          535 g         1151 t
ORIGIN      
        1 aaaaaagtac ttatccacag aaagacctta aattttcctc tacacattat ctcctgtatg
       61 attttttagc attgtcaatg tattcagaat aattattgat ttttacagca tatattccga
      121 ctggtataca tttatgattg gtacatccca tagctagtag catttaacca aatatgtatg
      181 cactatggca atcgtgcgcc agtagattac tgtattaatg catatactat gaacagtaaa
      241 cgaatagttc acatcgatta tctgaaagca tgggcagtaa ttgttatgat agaagtccat
      301 gttgttaatt gtttcctcaa tccatcattc aaaactgcat ggtggttcaa tatagtaaat
      361 ttcataaacg gacttgtggc tccagttttt atctttactg caggtttttc atttgtcttt
      421 tcagtaatga aaaagcgaga agaacttatc agtttatcag atttattttt tagaagaaca
      481 ggcagaatac tattaatagt tcttgtgggc tatcttatcc atatccccta cttctcatta
      541 aaaaatatta ttatttatgc ttcacctgct gaacttaaat ccttttattc agcagatgtt
      601 ctccaatgca ttggatcagg tttgctggtg ttactatttc tacgaatggt aattccgtca
      661 gatgtgctgt accatagtat catcaccggc atcggtttgt tgattgtttt tataacaccc
      721 tatatatggg agattgactt ctaccaatgg ttcccattgc caattgctac atattttaat
      781 gaaatgcatg gatcattatt cccagtattt ccatggcatg gatttttgtt tttaggcgca
      841 gcatatgggg tttttcaatt gcaccataac actaatatcc atgcagacaa atctttaaat
      901 acatttgcag caatttcttt tattattagc attgctggca ttatttctat gcatttctta
      961 tcccaaaacc ccgcatatac cataaagaca agtgcaatct tttttataac acggtatgca
     1021 ctcgtcatag tgctgctgca ttgctgccag tggtatcttc atataaaaaa taatcagggc
     1081 ggattattca tcaatatagg taaagaatca ctgcttgttt actggttaca tcttcagata
     1141 ttatacaggc atattttcaa tgggaaatca cttgaaagaa ttgttgctca gaaatttagc
     1201 tttgcagagt gtttgacagc ttttcttttg ctggcagcct tgatgtttgc agtagcaatt
     1261 atgtggaata cattaaaaca acattatccc gatatttccc gaaaaatatt tattggaaca
     1321 atactaattg gattaataaa atttttatct ttttaatctt aaactgcttt tgtggccttt
     1381 acaaggtact gtaacgcagt ttgattgttc cccacaaata aatggcacag acttaacgat
     1441 atgaatgcct gtggtgtagg ctctttctgt atttcctgct ctaattgctg tatcttggta
     1501 tcgatagtcc ccttgctgtc attgaaaatg gtgtgaggta ttgttgaaat atattcatta
     1561 aattgcctga atcccttttt ctgcaatgaa tgggatataa gcttttcatc attatactgt
     1621 gcacactgta aaattatatc tgaagtaatt tccctgaacc tgaatgagtc atggtaccta
     1681 tcatattgct gtaaatagcg taaggcatcg gtataatttt tttgttcata atataaaagt
     1741 ataattgcgg tcagggcaac tttatttgca gggtccttct taataacttc cttaaaacat
     1801 ccaagagcag ctttataatt attaattttg ctgaaagcta tacctttaac caggagagca
     1861 atataaaaat ctgggttcga tgtactacac ttattcagga taataatggc ttttttatat
     1921 tcacccaatc taaaataaag gcttccaatg ttgaataaaa caatataatc ttccttcagt
     1981 tgcagtgcac gtaaaaaaca atcaagagca tgattgaatt gtttcatgcg ataatacaat
     2041 gaacctaaat tgattaatgc agccctgaaa gaattatcaa tttggagtat ctgtatatat
     2101 aattgcaatg cttcagtaac attaccctgc tcttccagcc gaagtgcttc tataaattgt
     2161 tcctgtatag tcattcaatc aacctttcca aacaaatagg cattctattg cttactatcg
     2221 gcattttatt tggcaagcgc aactttattt cgtcccatct cttttgcctt atataatgct
     2281 ttatcagatc tgcctacaaa ttcctttata gaagtatctc ttgcaggatc atactgagta
     2341 actccaatgc ttatagtaac atgcaacgta ctgtcctgcc ccttgaatgg atgctgttca
     2401 acaatcttac gcaagcgttg tgccacaata cctgcctgat ccagtcgtgt ctggggcaat
     2461 atcaccgcaa actcttcgcc tccgtatcgg cagggtatat ccattttacg aaggtctttc
     2521 agaaatattg cagcaacttc tttcagtaca ctatcgccct gcaaatgccc ataggtatcg
     2581 ttgaaccgct taaaatggtc tatgtcacac atcaacagtg aaaggggtgc attatatcgc
     2641 tgacttcgtt tcatttcctc aaacaatcgt atttcaaaat aatggtgaat atacaaccgt
     2701 gtcattctat ccagtgttga cattaaatac agccgtgaat tttcaactgc caccgcgcca
     2761 aaccgtgcta cataatgtaa aaattccttt tctgaatttg aaaaaccacc accggttatt
     2821 ttttccccta aaagtagaac acccatcagt gaattttttg atttcaaagg tactactatg
     2881 tatggattta aaaacgctat caatttgagg tcatcagcca ggtcgggtaa ctccaggagt
     2941 gtttcatatt caattgggta tggattattt tctaaaaatc caacgagtgg acttgtttcc
     3001 tgcaatatca cattttcctc aaaatcactg tcataccctt tggccatatg tatataaaaa
     3061 tcattcacat caatgtcttt ttgcaacagc agcgctacct gttcaacaaa catctggcca
     3121 atacagctgt ataaaaggga ttcaaccagg ctttcaaact gcaaatttga tgaaagactt
     3181 attcctaact caagcaggtt gcgcaggtca taaatctttt tttccaggcc ttctatgcct
     3241 tccggtgttg gttcagtttc atcaaaaaaa gttaacccat tatcatccat acataccctg
     3301 taaaataatt cttgcgtttt tattacactt aac
//
LOCUS       DAOSZP010000211         3332 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_22664_length_3332_cov_2.781813, whole genome shotgun sequence.
ACCESSION   DAOSZP010000211 DAOSZP010000000
VERSION     DAOSZP010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3332
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22664_length_3332_cov_2.781813"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            65..1096
                     /locus_tag="PK348_05595"
     CDS             65..1096
                     /locus_tag="PK348_05595"
                     /inference="COORDINATES: protein motif:HMM:NF024891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXK65720.1"
                     /translation="MNKIVFACTRRSAGKTTIFTGLAKAMGKEVAYMKPFGDRLIYRK
                     KRLWDYDAALLTNLFGLEENPEDMTIGFEHAKLRYVYDEARIKEKLQEVAKSIAKNRE
                     VLFIEGGKDLAYGVSVYLDPITVAKSLDAKLVLIVGGNDDVIIDDITLFKKYVDTSDI
                     NFLGVIINKIHDLDDFKSTYADKIEKMGVPILGMIPYAADLAYFSMDYLSEALFAKVI
                     AGEAGLNRVVKNVFLGAMSVNEALRNPLFNKEGKLLITSGDRSDIIISALETNTVGII
                     LTNNILPPSNIISKAAEKNIPLLLVSSNAYQVAGQIDKMEALLTKDNKEKIALAEKLV
                     KENVKVSAL"
     gene            1382..1750
                     /locus_tag="PK348_05600"
     CDS             1382..1750
                     /locus_tag="PK348_05600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65721.1"
                     /translation="MATPYMLQNMYDSSVYLCQERIWHQIIDTAFQRGFQPVGTRLDY
                     YYELDLVWDTETTFMEKIFTSIMTHARCLNWNKYNFKDRENQIVCDEDCSELLYVLQD
                     ILPQDLKDFFSKGSFRICSD"
     gene            1783..2505
                     /locus_tag="PK348_05605"
     CDS             1783..2505
                     /locus_tag="PK348_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015905547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HXK65722.1"
                     /translation="MAIVEWKKDENIAIITMNTTENRHNVMFAQAMLQALDEIEADKE
                     INGIVLTSSDAKNFSLGVDIEWLMPRMAEKDFKAIKDFMYGMNNVFKRLLLIPMPVVA
                     AINGHAFGNGAILSCACDFRFMRSDRGFFCFPEVDLGIPFLPGMIAFVKKAIPYYKFN
                     EMKLTGRRVGAPELAEHHVIEKACANVEELMKESIAFAKSFKKKRGIFGEHKKRLHKH
                     IIEVIDKEDPEFIESLFLFIQD"
     gene            2650..>3332
                     /locus_tag="PK348_05610"
     CDS             2650..>3332
                     /locus_tag="PK348_05610"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXK65723.1"
                     /translation="MSLDPSVKKVLELLKNIELSSLTVEQARKLMDMGIDRQIKEDVK
                     STSDFKITYNDISLSCRLYEPFTTTDALIIYYHGGGFMFGNIELFDHVCRKATNSSGC
                     KVISVEYRLAPEHKFPAAVDDAYHSFRWIKDHAKNFDINPSKIAIAGDSAGANLATVT
                     CLRCKDTSMDLPKLQVLFYPVVAPDVCSESVRQFGDGFFLTRASMKWFTQLYLSSPAD
                     FINPYMFPI"
BASE COUNT         1084 a          583 c          658 g         1007 t
ORIGIN      
        1 ggcaaaggcc ttaaagctgt agacgtcagg ataataattt ctaaatcgta agtgaggtgt
       61 aattatgaat aaaatagtat ttgcttgtac acgaagaagc gccgggaaaa caaccatttt
      121 cactggtctg gcaaaagcaa tgggcaagga agtagcatat atgaagccct ttggcgatag
      181 actgatatac aggaaaaaac ggttatggga ctatgatgct gcacttctga ctaatctttt
      241 tggcctggaa gaaaatcctg aagatatgac cattggattt gagcatgcca agctgcgcta
      301 tgtgtatgat gaagcccgga ttaaagaaaa gcttcaggaa gtggcaaaat caattgccaa
      361 aaaccgtgaa gttctcttca tcgaaggtgg caaagattta gcctatggcg tttcagtata
      421 tcttgaccca ataactgttg caaaatcact tgacgcaaag cttgttctca ttgttggtgg
      481 caatgatgat gtcatcattg atgacattac ccttttcaaa aagtatgttg atacaagtga
      541 tattaacttc ctgggtgtta tcatcaacaa gatacacgat cttgatgatt ttaagagcac
      601 ctatgctgat aaaattgaaa aaatgggcgt tcctatttta ggaatgattc cctatgcagc
      661 agatcttgcc tacttctcca tggactatct gtcagaagca ttgtttgcta aagttatagc
      721 tggtgaagct ggcttaaata gggttgtgaa gaatgtattt cttggtgcaa tgtcagtcaa
      781 tgaagcactg cgaaatcctc ttttcaataa agaaggcaag ctcttgataa caagcggcga
      841 cagaagtgac atcatcattt ctgcacttga aacaaatact gttggtatca ttttaaccaa
      901 caatattctg ccaccttcaa acatcatttc aaaagcagct gaaaagaaca tcccgctgct
      961 tttagtttca agcaatgctt atcaggtagc aggccagatt gacaagatgg aagctttatt
     1021 aaccaaagat aataaggaaa agattgctct ggctgaaaag ctggtaaaag aaaatgtgaa
     1081 agtttctgct ctatagacgc aattgacttt tacaaaaaaa aggggctaca aaagcccctt
     1141 ttttttgcat ttattgtaat ttttagatta cactgtgcca tcaacataac agccatgaag
     1201 atagtgatta taatcatctt tcccttactt ctcaatacaa ttatgataag gatgtgactg
     1261 tagtatataa ttaaagaact catcaattta attttttgtc ggtataaagt tccttgacag
     1321 ggcataacgt tgttatgcta tagtaattgg agtttatcaa aactatacca atgtgtcaga
     1381 tatggcaaca ccatatatgt tacaaaatat gtatgatagc tctgtgtatc tgtgccagga
     1441 acgtatctgg catcagataa ttgatacggc ctttcagcgg ggatttcagc ctgtgggtac
     1501 acggcttgac tattattacg agttagatct ggtctgggat actgagacca catttatgga
     1561 aaaaatattt acatctatca tgacacatgc acgctgccta aactggaata agtataattt
     1621 caaagacaga gaaaaccaga tagtatgcga tgaagactgc agcgaactat tgtatgtttt
     1681 acaggatatc ctgccgcagg atttaaaaga ttttttttcc aaaggctctt ttagaatatg
     1741 cagtgactaa taactaaatt taaaactaaa gggggtactt atatggccat tgtagaatgg
     1801 aaaaaggatg aaaacatagc aattatcacc atgaacacaa cagaaaaccg ccataacgtg
     1861 atgtttgcac aggcaatgtt gcaggctctg gatgaaattg aagctgataa ggaaattaat
     1921 ggtattgtgc ttacatcaag tgatgcaaag aatttcagtt taggtgttga tattgaatgg
     1981 ctgatgccac gtatggcaga aaaagatttc aaggcaatca aagatttcat gtatggcatg
     2041 aataatgtgt ttaagcgcct gctgcttata cccatgccgg ttgttgcagc cataaacggg
     2101 catgcttttg gcaatggagc aatcctttca tgtgcatgcg atttcagatt catgcgctcc
     2161 gacagaggat ttttctgctt tcctgaagtt gatttaggaa tcccattttt gccaggtatg
     2221 atcgcgtttg ttaaaaaagc tattccctac tataaattca atgagatgaa actcaccggc
     2281 aggcgtgtag gagcacctga gcttgccgaa caccatgtga ttgaaaaagc ctgcgccaat
     2341 gtagaggaac tcatgaagga atcaattgca tttgcaaaat cattcaagaa aaaacgtggc
     2401 atttttggcg agcataaaaa aaggcttcac aaacatataa tcgaagttat agataaggaa
     2461 gaccctgagt ttattgaatc attgttttta tttattcagg attaacactt caaattaaca
     2521 ttaatattga aaaccctcgc ttcccatagt gagggttttt taatataaaa aatttcgttg
     2581 acatattaat gtccattcat taatttttac agtaactatc gcacacaaaa aaacagttgg
     2641 aggtcttcaa tgtcattaga tccttctgta aaaaaagttc tggaattgtt aaaaaatatt
     2701 gaattaagca gtttgaccgt tgaacaggcc cgcaaattga tggatatggg tattgatagg
     2761 cagatcaaag aagatgtaaa atcaaccagt gattttaaaa taacctacaa tgatatttct
     2821 ctttcttgcc gtctatatga gccatttacc actactgatg cacttattat atattaccat
     2881 ggtggcggat ttatgtttgg taatattgaa ctttttgacc atgtatgcag gaaagcaacc
     2941 aattcttcag gttgtaaagt catatcagtt gaatacagat tagctccaga acataaattc
     3001 cccgctgctg ttgatgatgc ataccattca tttagatgga ttaaagatca tgctaaaaac
     3061 tttgatatta atccttcaaa aattgctata gcaggcgaca gtgctggcgc caacttagcc
     3121 acagtaacct gtcttcgatg taaagatacc agcatggatt tacccaaact tcaggtactc
     3181 ttttatccag ttgtggcacc tgatgtatgt tcagaatcag tacggcaatt tggagatgga
     3241 ttttttttaa ccagagcatc aatgaaatgg ttcacacagc tatatctttc atcacctgct
     3301 gattttatta atccttacat gttcccaatc tt
//
LOCUS       DAOSZP010000212         3289 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_22979_length_3289_cov_4.227273, whole genome shotgun sequence.
ACCESSION   DAOSZP010000212 DAOSZP010000000
VERSION     DAOSZP010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3289
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22979_length_3289_cov_4.227273"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1185
                     /locus_tag="PK348_05615"
     CDS             <1..1185
                     /locus_tag="PK348_05615"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HXK65724.1"
                     /translation="MAGVMVLFGLIMIAGAVIGIYYIIAMRLNPLERLKEIAGALAGG
                     NVTMRYEGKLYNDEVGDMAKAINNTMDNLERLVAEVKVAVSNLTQAVNEIANGNENLS
                     QRTSEQASSLEEIAATIEEATATIKQNAEHATRANQIAEKTSELAKNGNNLVSDAVVA
                     INEINASSKRIGEILTLINEISFQTNLLALNAAVEAARAGEQGRGFAVVAGEVRNLAQ
                     RSGSAAKEIGELIRDSLEKIDTGTTLVNKSGEALKEIMQSIQELYKTITEIATASDEQ
                     KQGIDQINVAVSDLDTMTQQNAALVEETASASEEMANQAQELASMMERFVIREEISEK
                     VFGSKHRELHLHAAEVGKKVGVKRVEKPKAKEEKKEAATSEEKKEKGPNNKLIDDGFE
                     EF"
     gene            1209..>2419
                     /locus_tag="PK348_05620"
     CDS             1209..>2419
                     /locus_tag="PK348_05620"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HXK65725.1"
                     /translation="MADDAIKQVFLAETKELLENLENDIVRYEETKDPGLIHSIFRYV
                     HTLKGGSGMAGYEDVYQFTHALENILDSVRNGILPMNDLLADIVFASIDVIRLRLYSA
                     EDPSKFEEKFALLQAKINDIESILQVKEEETVEEEKTEEIQPVYNFFRVKAKFREDIF
                     KSGIDPLIIMEDLASLGKITELRVNRNDVPLLENLDPEKCYLSWDIILKTKSKEEALD
                     DVFIFVRDDNEISITNVTKNYVAQPVVEDVPKLGELLLSKGMLTEKEYDDVLSEHEKT
                     KKKVGEIAVTKGYVEDNDISLALKEQESIKKHLSTSTLRVDAKKVDSLMNLLGEMVIG
                     FSGIKRIAEEIEDEKAYRLNNALYGVDRIIREFQEHLMRIRMVPIGPVFEQFRRFIRD
                     TAKSHGKLIHL"
     assembly_gap    2420..2429
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2430..>3289
                     /locus_tag="PK348_05625"
     CDS             <2430..>3289
                     /locus_tag="PK348_05625"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HXK65726.1"
                     /translation="IAGGDTEIDKTVIERINDPLKHLIRNAIDHGIESPQQRQAKGKP
                     QEGTILLKAYHQEGNIFIEISDDGQGIDREKVLQKVMASGISSKLDDLPDAKIYEFLF
                     APGFSTAESVGDLSGRGVGLDVVKTNIEALRGSVEVFSEADKGTTFRIKLPLTLAIID
                     GMLVKVAHETFIIPILSIIESIKPGKKDIKTVENKGEVVHVRGEYVPLVRLYKEFKLP
                     QTIENPWDGLVVIVESEGKALGLLVDELVGQQQIVIKSLDNQITLSRAISGAAILGDG
                     TIALIIDVHG"
BASE COUNT         1110 a          500 c          832 g          837 t
ORIGIN      
        1 atggctggag ttatggtgct atttgggctt atcatgatag caggtgcagt aataggtatt
       61 tactacatta ttgcaatgcg tttaaaccca ttagaacggc ttaaagagat agcaggtgct
      121 cttgctggtg gcaatgtgac catgcgctat gaaggtaagc tatataacga tgaagttggt
      181 gatatggcaa aggctataaa taataccatg gataacctgg agcgtctggt tgcagaggtt
      241 aaggtggccg tgagcaatct aacgcaggca gtcaatgaga tagccaatgg caatgaaaac
      301 ctgtcgcagc gtacaagtga gcaggcatca tcccttgaag agatagcagc aaccatagaa
      361 gaggcaacag caaccataaa gcagaatgca gagcatgcaa cacgggcaaa ccagattgcc
      421 gagaagacga gcgagcttgc caaaaatggt aacaatttag tcagtgatgc tgttgtggca
      481 ataaatgaaa tcaatgcatc aagtaagcgc attggcgaga tattaaccct tatcaatgag
      541 atatcattcc agacaaattt actggcactg aatgcagcag ttgaagcagc acgagcagga
      601 gagcaggggc gtggttttgc ggtggtagca ggtgaggtgc gtaatttagc gcagcgttcg
      661 ggcagtgcag ccaaggagat tggtgagctt atacgcgatt cactggaaaa gatagataca
      721 ggcacaacgc tggtaaacaa gagtggtgaa gcattaaaag agattatgca gtcaatacag
      781 gaattgtata agacaataac cgagatagca acagccagtg acgaacagaa acagggtata
      841 gaccagatca atgtagcagt atcggattta gataccatga cgcagcagaa tgcagcgttg
      901 gtggaagaga cagccagcgc cagtgaggag atggcaaatc aggcacagga gcttgcaagc
      961 atgatggagc ggtttgtgat acgtgaagaa ataagtgaaa aagtatttgg gagtaagcac
     1021 agggagttgc atttgcatgc tgcagaggtg ggtaagaagg tgggagtaaa gagggtggaa
     1081 aagccaaagg caaaagagga aaagaaggaa gcagcaacat cagaggaaaa gaaagaaaaa
     1141 ggtcccaaca ataaacttat agatgatggg tttgaagagt tttaataaca gttactggag
     1201 gaaaatacat ggcggatgat gccataaagc aggttttttt agctgaaaca aaagaactgc
     1261 tggaaaactt agaaaatgat atagtccgtt atgaggaaac aaaagaccct ggattgattc
     1321 attcaatatt caggtatgtg catacattaa agggtggttc ggggatggca gggtatgagg
     1381 atgtatatca atttactcat gccctggaaa atattttgga ttcagtgcgc aatggtattc
     1441 ttcccatgaa tgatctttta gcggatatag tgtttgccag cattgatgta atcagattgc
     1501 gcctgtattc ggcagaagat ccatcgaagt ttgaggaaaa gtttgcgtta ttacaggcaa
     1561 aaattaatga tattgaatca attcttcagg tgaaagaaga agaaaccgtt gaagaagaaa
     1621 aaactgaaga aatacagcca gtatataatt ttttcagggt taaagctaaa ttcagagaag
     1681 atatatttaa atcgggtatt gatccgctta ttatcatgga agacttagct tctttaggga
     1741 agattactga gttacgtgtt aaccgaaatg atgtgccact gcttgaaaat cttgatcctg
     1801 aaaaatgtta cctgtcgtgg gatattatcc ttaaaaccaa aagcaaagaa gaagctcttg
     1861 atgatgtatt tatttttgtg cgtgatgata atgagatatc cataaccaat gtaaccaaaa
     1921 attatgttgc acagcctgtt gtagaggatg tccccaaatt gggtgagtta ttattgtcaa
     1981 agggcatgct tactgaaaag gaatatgacg atgtattgag cgaacacgaa aagacaaaga
     2041 aaaaagttgg tgagatagct gttaccaaag gatatgtgga ggacaatgat atatcactgg
     2101 cactcaagga acaggagagt attaaaaagc atctgagtac ttcaacattg agagttgatg
     2161 caaaaaaagt tgatagcctc atgaaccttt taggtgaaat ggtcattggt ttttcgggga
     2221 ttaaacggat agctgaagag atagaagatg aaaaggcata tcgtttaaac aacgccctgt
     2281 atggtgttga ccggataatc cgtgaatttc aggaacatct tatgcgaatt cgcatggttc
     2341 ctattgggcc ggtgtttgag caattcaggc gttttatccg tgatactgca aaatcgcatg
     2401 gcaagttgat acacctggan nnnnnnnnna attgcaggtg gtgatacaga aattgataaa
     2461 actgtcattg aacgcatcaa tgatcctcta aagcatttga ttcgtaatgc gatagatcat
     2521 ggaatagaaa gtcctcagca acgtcaggct aaagggaagc ctcaggaagg tacaatattg
     2581 ctgaaagcgt atcatcagga aggaaatatt tttattgaga taagtgatga tggacagggt
     2641 attgatagag aaaaggtatt acaaaaagtt atggcatcag gaatttcaag taaacttgat
     2701 gatttgccag atgcaaaaat atatgaattt ttgtttgcac ccggtttttc tactgccgaa
     2761 tcagttggtg acctttccgg aagaggtgtt ggcctggatg tggtcaaaac aaacattgaa
     2821 gcattgcgtg gcagtgttga agtcttcagt gaagccgata agggcacaac atttagaata
     2881 aagcttcctt tgacactggc aataatagat ggtatgctgg tgaaagtggc gcatgaaaca
     2941 tttattattc caatactttc gataattgaa agcatcaaac ctgggaaaaa agacataaaa
     3001 actgttgaga ataaaggcga agttgtccat gtcagaggag aatatgttcc gttagtcagg
     3061 ctttataaag aatttaagct gccacaaact attgaaaatc catgggatgg ccttgtggtt
     3121 atagttgaat cagaaggaaa ggctctggga ctactcgttg atgaacttgt tggtcagcaa
     3181 cagattgtga taaagagtct ggataatcaa ataactttat caagagctat atcaggtgcc
     3241 gcaatattag gtgatggaac tatcgccctt attattgatg tacatggtt
//
LOCUS       DAOSZP010000213         3282 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_23047_length_3282_cov_3.231174, whole genome shotgun sequence.
ACCESSION   DAOSZP010000213 DAOSZP010000000
VERSION     DAOSZP010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3282
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23047_length_3282_cov_3.231174"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1732)
                     /gene="fusA"
                     /locus_tag="PK348_05630"
     CDS             complement(<1..1732)
                     /gene="fusA"
                     /locus_tag="PK348_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015838551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HXK65727.1"
                     /translation="MGRELPLHRTRNIGIMAHIDAGKTTLTERILYYTGKTYKIGEVH
                     DGTAEMDWMIQEKERGITITAAATTTYWKNTRINIIDTPGHVDFTVEVERSLRVLDGA
                     IAVFDAVAGVEPQSETVWRQATHYNVPRICFVNKMDRVGADFENCLTMIKERLYSIPL
                     PLQIPYGKEDSFKGVIDVVQMKALVWNDQLGEHYDVVPIPEEYHDEALYYREHLIELV
                     AEYDEEMLEKYLEGVEITETELKRAIRNATLKTALFPVFCGSALKNKGVQPLIDAITD
                     YLPSPKDIPAIRGHDVHHEDTIMERHPDDKEPFCALVFKIASDPYVGKLCYIRVYSGH
                     LAAGDTVLNVDKQKKERIGRILRMHANDREDIKEIYTGDIAALVGLRFAQTGDTITAV
                     DAPILLEKMDFPEPVIARAVEPKTRADLEKLHTVLKRLEEEDPTFFVKIDEESGQTII
                     SGMGELHLEIIVDRLQREFNVLVNVGKPQVAYKETVTAHAQAEGVFEKEIAGKLQYGH
                     VLLGISACDSSFEFVSKVDSTVVPENCIKAVEMGCRDALSSGVIAGYEMTKVRVVLED
                     AKYREDASADH"
     gene            complement(1837..>2251)
                     /gene="rpsG"
                     /locus_tag="PK348_05635"
     CDS             complement(1837..>2251)
                     /gene="rpsG"
                     /locus_tag="PK348_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015851064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HXK65728.1"
                     /translation="NIVVSKFINCMMLKGKKGVAEQAFYKALELIGERTQKDPIEVFI
                     TSLENVKPLVEVKSRRVGGATYQVPVEIRPERRQALAIRWLINNARSRNEKTMFQKLA
                     GELLDAYNNTGASIKKKEDTHKMAEANKAFAHYKW"
     assembly_gap    2252..2261
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2343..2717)
                     /gene="rpsL"
                     /locus_tag="PK348_05640"
     CDS             complement(2343..2717)
                     /gene="rpsL"
                     /locus_tag="PK348_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012538144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HXK65729.1"
                     /translation="MPTINQLIRKGRSEKVKKSKSPALKSCPQKRGVCTRVMTVTPKK
                     PNSALRKVARVRLTNGIEVTAYIPGIGHNLQEHSAVLVRGGRVKDLPGVRYHIVRGTL
                     DSLGVNDRKKGRSKYGTKRPKA"
     gene            complement(2835..>3282)
                     /locus_tag="PK348_05645"
     CDS             complement(2835..>3282)
                     /locus_tag="PK348_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004783957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HXK65730.1"
                     /translation="QINDKHIEIIVRQMLRKVIIEDPGDTSFVVDQIVDKFEFDDENM
                     RVEKEGGKAASAKPVLMGITKSALNTESFISAASFQETTRVLTEAAIKGKIDRLRGLK
                     ENVIIGHLIPAGTGVREYNKVTVYQEVKGDLDGVAKEVEEEIPAEK"
BASE COUNT          836 a          810 c          566 g         1060 t
ORIGIN      
        1 catgatcagc cgaggcatcc tcacggtact tggcgtcctc aagaacaacc cgtaccttgg
       61 tcatctcgta gccggctatt accccggaac tgagcgcatc acggcaaccc atctccacag
      121 cttttatgca attttcgggt accactgtgg aatccacctt tgagacaaat tcaaaggagc
      181 tatcgcacgc acttatccca agcagaacat gaccatactg cagtttaccg gcaatctctt
      241 tttcaaatac tccttcagcc tgtgcatgtg ctgttactgt ctccttgtag gcaacctgag
      301 gtttaccaac attgacaagc acattgaact cgcgctgcag cctatcaaca ataatttcca
      361 gatgcaactc acccatgcct gatatgatgg tttggccgct ttcctcatca attttaacaa
      421 aaaatgtcgg atcttcttct tccaagcgct tgagcaccgt atgtaatttt tccaggtcag
      481 ccctggtttt aggttcaacc gcacgtgcta tcaccggctc agggaaatcc atcttttcaa
      541 gaagtattgg cgcatcaaca gcagtaatcg tatcgcctgt ctgggcaaac cgcaaaccta
      601 caagcgcggc tatatctccg gtatatattt cctttatgtc ttcccggtca ttggcatgca
      661 ttctaagtat ccgaccaata cgttccttct tctgcttgtc aacattcagg acagtatcgc
      721 cagctgcaag gtgccctgaa taaaccctga tatagcagag cttgcccaca tacgggtcgc
      781 ttgcaatttt aaacaccagg gcacagaatg gctccttgtc atccggatga cgctccatta
      841 ttgtatcttc atgatgtaca tcatgtcctc tgatagccgg aatgtcttta ggcgaaggca
      901 gataatcagt aatggcatca attaaaggct gtacaccttt atttttcagc gcgcttccac
      961 aaaataccgg aaacaatgct gttttcaacg ttgcattgcg tatggcccgt ttcagttcag
     1021 tttccgttat ttccacacct tccagatatt tttccagcat ttcctcatcg tattctgcaa
     1081 ccagttcaat cagatgttca cgataataca aagcttcatc atggtattct tcaggtattg
     1141 gaacaacgtc atagtgctca cctaactggt cattccacac cagtgctttc atctgcacta
     1201 catcaataac acctttgaag ctatcttcct tcccataggg aatttgtaag ggcagcggta
     1261 tggaatacag gcgttccttt atcatggtga gacaattctc aaaatctgct cccactctgt
     1321 ccattttatt tacaaaacat attctgggta cattgtagtg tgtagcctgt cgccatactg
     1381 tttcactctg aggctccact ccggcaacgg catcaaatac ggctatagct ccgtctaaaa
     1441 ctctcagact tcgttccacc tcaacggtaa aatcaacatg tcccggagtg tctatgatat
     1501 tgatacgcgt atttttccag taggttgtgg tagcagcagc agtgatggta atccctcgct
     1561 ccttttcctg aatcatccag tccatctctg cagtgccatc atggacttct ccaattttat
     1621 aggtcttgcc agtataatag aggatgcgct cggttaatgt ggttttacct gcatcaatat
     1681 gagccattat cccaatattt cgtgtcctgt gtaatggtaa ctctcttccc ataatacctg
     1741 atgataaaat taattattta tgataaagca tcaaatacca gggggatgcc ctggtatttt
     1801 atatattaaa cttttaaaca gcaaataata ctataattac cacttataat gtgcaaatgc
     1861 cttgttagct tctgccatct tgtgagtgtc ttcctttttc tttattgaag caccggtgtt
     1921 gttgtatgca tccagtagtt ctcccgccag tttctgaaac atggtctttt catttctgct
     1981 gcgggcattg tttataagcc acctgatagc aagtgcctgg cgcctttctg gccgtatttc
     2041 aacaggtacc tgatacgttg caccaccaac acgtcgtgat ttaacctcaa caagaggctt
     2101 tacattttca agcgaagtaa taaatacttc aattgggtct ttctgagtac gctcgccaat
     2161 gagttccaat gctttataaa aagcctgctc agcaacgcct tttttgcctt ttaacatcat
     2221 gcaatttata aattttgaaa ctactatatt gnnnnnnnnn ntgggtccgg taaaattgtc
     2281 ctttttgctg gtctacgcct tcgtgccatt attaaacctc actataccaa ataattatta
     2341 cattatgctt ttggccgctt ggtgccgtat tttgatcttc cttttttgcg gtcattaact
     2401 cctaaagaat ccaatgtccc acgtactata tggtacctca cacctggcag gtcctttacc
     2461 cttccacctc gaactaacac agcagagtgt tcctgaagat tatggcctat acctggaata
     2521 tatgctgtaa cttctattcc attggttagt cttacacgag caaccttccg caatgctgaa
     2581 ttaggttttt ttggcgtaac agtcattact cgtgtacata ctcctctttt ctgagggcaa
     2641 ctcttcagtg ccggggattt tgatttctta accttttcag atctcccttt ccttattaac
     2701 tgattaattg taggcatcct gtctcctcac tacatttatt gaatattata acattatatt
     2761 atacagatta atcactttca aaattttaat tgaaagtgat taatcaattt tacctgtaga
     2821 attgtcaaga aattttattt ctcagcaggg atttcctcct ctacttcctt tgcaacgcca
     2881 tctaagtcgc ccttaacttc ctggtataca gtaaccttat tgtactcacg tacgccagta
     2941 cctgccggaa tcaaatgacc aatgattaca ttttccttta atccacgtaa tctatcaatc
     3001 tttcctttaa ttgccgcttc agttaacacg cgggttgttt cctggaacga tgcagcagat
     3061 ataaacgatt ccgtattaag agctgatttt gttataccca tcagtaccgg ttttgcagat
     3121 gctgctttac caccttcttt ttcaacacgc atattttcat catcaaattc gaacttatca
     3181 actatctggt ctacgacaaa gcttgtatca ccaggatctt ctatgatgac tttccgtaac
     3241 atctgtcgca caattatctc aatgtgctta tcatttatct ga
//
LOCUS       DAOSZP010000214         3273 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_23130_length_3273_cov_3.345556, whole genome shotgun sequence.
ACCESSION   DAOSZP010000214 DAOSZP010000000
VERSION     DAOSZP010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3273
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23130_length_3273_cov_3.345556"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="PK348_05650"
     CDS             <1..369
                     /locus_tag="PK348_05650"
                     /inference="COORDINATES: protein motif:HMM:NF013514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HXK65731.1"
                     /translation="EKNINGATYFAITTLLQQRPHTPHTVLLDGNFSFPLSLPYKSII
                     KGDCLSISIASASIIAKVTRDEYMISINETYPEYGFAHHKGYATAKHIDAIKKYGPSP
                     LHRKTYEPVARLFYELSLFP"
     gene            366..1340
                     /locus_tag="PK348_05655"
     CDS             366..1340
                     /locus_tag="PK348_05655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65732.1"
                     /translation="MKITDNIVSAHRTHSSAITLKSGQIVVGTITKRLTANTAMVRVG
                     NQIFETRFARGLPQSNHILLKFDTITGNRFIFSLVEPKALGNTNILSATPSNYALFMR
                     NLNAIDSLTVFSIHKALFDTNANREYTFTVIAPLLQRLLQKGVPERLLQKFCYTLSKD
                     TGISLTMHILQNIGDSSLQDFELHDDDIESLMSMMSKDDIESLVQYLGDSSGNVFTVP
                     LYDGNSFSDAYILSDDNYVCIDVTFSHLGKLNIMITGKDTIAATIYCFTDAAFSQLHQ
                     QCPIIARSIHEQTGKKAACSCIQRSDWEEKIIALINNMYIQYSIDFSV"
     gene            1352..1618
                     /locus_tag="PK348_05660"
     CDS             1352..1618
                     /locus_tag="PK348_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013909478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HXK65733.1"
                     /translation="MENIAVALQYSVEEHIPKVIAKGKGKLAKSILDKAKEYNIPIYQ
                     DKDLVAVLSSLETGSYIPESLYRAVAVVLAYCYQINSDFRKKLN"
     gene            1693..2883
                     /locus_tag="PK348_05665"
     CDS             1693..2883
                     /locus_tag="PK348_05665"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF023299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HXK65734.1"
                     /translation="MAEMRKIPVDELKPGMRFDKPVYIDSNNMLVAANVSIKLDDIKK
                     LMRWGVSEVETAGNLVSTEQEIKAATARATMTESDEAKKIIDDYNKLLTQRVPLIEIH
                     KDACKAVGNVHNAIKADQQFAIDEIDVSLTKIMEIMKNYPNIFLFLYGLEEGKNYLVV
                     HSVNVTFYSLLIGMALKYTPLKLRELGIGTMLIDAGMIKLPAYIMHKQSNLTEHEFNQ
                     IKTHPLHGYKALRQLGKIPEKSALVCLQHHEQYDGKGYPRGLKGTQIDEYARIAAIAD
                     SYEAQIANRAYRKRVFFYQAMKNLLSSGINKFDPVILRIFLSRMSVYPIGSIVELSNG
                     TIGIVIGSIPQKPLRPIVKLIIDSNKKRFTDTIIVNLLEEKALYITRALDEAEIGVNL
                     FEVL"
     gene            2915..3268
                     /locus_tag="PK348_05670"
     CDS             2915..3268
                     /locus_tag="PK348_05670"
                     /inference="COORDINATES: protein motif:HMM:NF009150.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HXK65735.1"
                     /translation="MNNHQYGMQGETLAKDYIEKQGFSVIEQNFRYGRYGEIDIIGIK
                     DTLLVFFEVKTRHTLAYGNPSYAITNKKKKALRAVATHFLIQNPHYNSNQYTLRFDCI
                     TILGQTIEWIQDIVR"
BASE COUNT         1101 a          628 c          617 g          927 t
ORIGIN      
        1 gaaaagaata taaacggagc cacatatttt gccatcacta cgcttttgca acaacgccca
       61 cacacacccc acacagtgct tcttgatggc aatttttctt ttccactatc gctgccgtat
      121 aagtccatta tcaagggtga ttgcctgtcg atttccattg catcagcatc aatcattgcc
      181 aaggtaaccc gtgatgaata tatgattagc attaatgaaa catatcctga atatggcttt
      241 gcacaccata aagggtatgc aactgcaaag catattgatg ccataaaaaa atacggacca
      301 tcgcctctgc accggaagac ctatgaaccg gttgcacgtt tattctatga actatcgcta
      361 tttccatgaa aattacagac aacattgtta gcgcacaccg tacgcactca tcagccatta
      421 cactgaaaag cggtcagatt gttgtaggta ccatcacaaa acgtttaaca gcaaacaccg
      481 ctatggtgcg tgtgggaaat cagatttttg aaacacgttt tgccagaggg cttccacaat
      541 ccaaccatat attgctaaaa tttgatacaa taactggtaa ccgttttatt ttttcactgg
      601 ttgaacctaa agctctgggt aataccaata tactgagtgc aacaccctca aactatgcat
      661 tatttatgcg aaacctcaat gctatagatt cgctgactgt gttttccata cacaaggcac
      721 tgtttgacac aaatgctaac cgggaatata catttacggt gattgccccg cttttacaac
      781 ggttgttgca aaaaggtgta ccggaaaggc tgctgcaaaa attttgttac actttatcta
      841 aagacactgg tataagttta actatgcata tattacaaaa tattggcgat agttcattac
      901 aggattttga gcttcatgat gatgatattg aaagccttat gtcaatgatg tcaaaagatg
      961 acattgaaag ccttgtacaa tatctgggcg atagctccgg aaatgtgttc acagtgccat
     1021 tgtatgatgg caattcattc agtgatgcat atattctttc cgatgataat tatgtgtgta
     1081 ttgatgtgac attttcacat ttagggaagt tgaatattat gataaccggg aaggatacga
     1141 tagctgctac catatactgt tttactgacg ctgccttcag ccaattacat cagcaatgcc
     1201 ctataatagc acgcagtatc catgaacaaa ccgggaaaaa agcagcctgt agctgtatac
     1261 aaagaagtga ttgggaagaa aaaattattg cattaattaa caatatgtat atacaatata
     1321 gtattgattt ttctgtatag gaaagtccat aatggaaaac atagctgtag cactacagta
     1381 ctcagttgaa gaacacatcc ccaaagtcat tgctaagggt aaaggaaaac ttgcaaaaag
     1441 tattcttgac aaagcaaaag agtataatat acctatttat caggacaaag atcttgtagc
     1501 ggtactttca tccctggaaa ccggttcata tatacctgaa agcctttaca gggctgttgc
     1561 agttgtactg gcgtattgct atcaaataaa cagtgatttt agaaaaaaat tgaattgaaa
     1621 taatacactt tttataatat taagttcata aacaatatgt gaataagggg ataacataaa
     1681 aatttgacac ctatggctga aatgcgaaaa ataccagttg atgaattaaa acccgggatg
     1741 aggtttgata aacccgttta tatagacagt aacaatatgc tggttgctgc taatgtgtcc
     1801 atcaagcttg atgatataaa aaaacttatg cggtgggggg tcagtgaggt tgaaactgct
     1861 ggcaatttag tgtcaaccga gcaggaaatc aaggcagcta ctgcccgggc taccatgacc
     1921 gaaagcgatg aagctaaaaa aatcattgat gattacaata agcttcttac ccaacgggtg
     1981 cccctgatag aaatacataa agatgcatgt aaagctgttg gaaatgtcca caacgccata
     2041 aaagcagatc aacagtttgc tattgatgaa attgatgttt ccttaacaaa aataatggaa
     2101 attatgaaga attatcccaa tatcttttta tttttatatg gactggaaga aggaaaaaat
     2161 tatttagttg ttcattccgt taacgtcaca ttttactcac tgcttattgg catggcattg
     2221 aaatatacac ctctgaaact tcgtgaactg ggaattggca ccatgcttat agatgccggt
     2281 atgattaaac tacccgcata cattatgcat aagcaatcca accttactga gcatgaattt
     2341 aaccagataa aaacccatcc tctgcatgga tataaagcac tgcgacaatt ggggaagata
     2401 cctgaaaaaa gtgcccttgt atgcctgcaa caccatgagc agtatgatgg taaaggatac
     2461 cccagaggct taaaaggcac acaaattgat gaatatgccc gtatagctgc aatagctgac
     2521 tcatacgaag cccagatagc aaacagagct taccgcaaac gtgtattctt ctatcaggca
     2581 atgaagaatt tgctttcaag cggaatcaat aaatttgatc ctgttatatt gcgtatattc
     2641 ctgtcaagga tgtcagtata tcctattggt tccatagttg agcttagcaa tggcaccata
     2701 ggaattgtta ttggttctat cccacaaaag ccattgcgtc ccattgttaa gctcattatt
     2761 gattcaaata aaaaacggtt caccgatacc attatcgtta accttcttga agaaaaagct
     2821 ctatatataa cacgggcact tgatgaagct gaaattggcg taaatctttt tgaagtactg
     2881 taatctaact atagatacat agcggagtat gccaatgaac aaccaccagt atggcatgca
     2941 aggagaaacc cttgcaaaag attatattga aaaacaggga ttttcagtta ttgaacagaa
     3001 ttttcgctat ggacgctatg gggaaattga catcattggc atcaaggata cattgcttgt
     3061 gttctttgag gttaaaacac gccatacatt agcttacggt aatccgtcgt acgctatcac
     3121 caataaaaag aaaaaagcac tgcgcgccgt ggcaacccat ttccttattc agaatccaca
     3181 ctacaatagt aaccagtata ctctacgatt tgattgtata accatattag gccaaacaat
     3241 tgaatggata caggatatag tacggtgaac tta
//
LOCUS       DAOSZP010000215         3265 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_23223_length_3265_cov_2.994081, whole genome shotgun sequence.
ACCESSION   DAOSZP010000215 DAOSZP010000000
VERSION     DAOSZP010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3265
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23223_length_3265_cov_2.994081"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..847)
                     /locus_tag="PK348_05675"
     CDS             complement(<1..847)
                     /locus_tag="PK348_05675"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HXK65736.1"
                     /translation="MKRLVLCFVAILLLYIPVFAQEGENVIQLDDVIVSDTVLYPGAY
                     YSISGSTVKDSVNTDLISVIKSQVPSLYTGSNRVMGYGVARSGAATISIRGISRSGWG
                     PTTGIPILYNGMDTTMGIMGHPVADIMTMKNVERIEVLIGPQPVLFGSSAMGGVINVI
                     TKKQKEDGFTTELQGSYGTWNTTEDYLYHMGKFGNIDYSVGYQFQKTDGDWKQTINGK
                     TYTSEFMEHNGTVHVGYTLSKNWYASVDSYGMKQDINDPGPEGMPAGQIGLLEEFDVT
                     RGGVVA"
     gene            complement(955..1341)
                     /locus_tag="PK348_05680"
     CDS             complement(955..1341)
                     /locus_tag="PK348_05680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65737.1"
                     /translation="MNKIIIYIVLLICAIVHSIGCSAKKDTQQTIKLVVVSAYNCDSC
                     SAIDDTIEYLQRIDRALEIKKVDIHNPEGAMYVSQYHLWRIPVYLFLDGNGMELYRLE
                     GEQSRSEIEKGLSIAKQRITNRNKPE"
     gene            complement(1351..2451)
                     /locus_tag="PK348_05685"
     CDS             complement(1351..2451)
                     /locus_tag="PK348_05685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65738.1"
                     /translation="MKIKGSHIAILLVIIIIVIIMLISKSENNNQPSTLSAGDSLKGY
                     GTVLEQMGLISPQQALQDYKQLAIYPPNSRPLSKENIDLLMPNRRYEQPLPIEKDSDI
                     YYLFTADKYRVIGDEPITFVLMVLQGTKPDSDRVPVTITKSIIKKGNNIKTAKYVSEL
                     SLIKNNVGDYTADVNCAQVFTSDKKSGTYWAQVEFKAADEVIDAAIPFEYFPENTIPA
                     KFTGNFRDYIKNGSFYIDVELDVLRKGYYIIDANLFDNELEPVAYTIEKRELQAGRQW
                     LPLLFFGKVLLDKKAKPPFVLRDLRGYRFIEATTPDPEFMDREMMKPYDGEYKTKVSS
                     LQGLSDKEWDDEQKRKRIEFLEKESMGDIGVQ"
     gene            complement(2648..>3265)
                     /locus_tag="PK348_05690"
     CDS             complement(2648..>3265)
                     /locus_tag="PK348_05690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65739.1"
                     /translation="TNWLDGKGTSRLASKSGYTSINCYVDNRNTIAYSASQFKTNYLD
                     VYCKGSNWCYIVNYSAGDVIMGYINANYTPQWNIAYVYTTAGAGGGSEIAIKGISDLF
                     TCNLAGQLGVSTVRNMYNHNDTNGITIYHIGGYKGIFGASVLLPGEDDGAVAYHSSGA
                     CVNSGSYNSLCTCTHWTNHVIAYTCTGYYLDHYGMKMKFIYQLGW"
BASE COUNT          981 a          692 c          476 g         1116 t
ORIGIN      
        1 tagccacaac gccaccacgc gtaacatcaa actcttctaa aagtccaatc tgtccagctg
       61 gcatcccttc tggtcccgga tcattgatat cttgcttcat gccatagcta tcaactgatg
      121 cataccagtt tttggataga gtatagccaa catgaactgt tccattatgc tccataaatt
      181 ctgatgtgta tgttttgcca tttattgtct gcttccagtc gccatcagtt ttttgaaatt
      241 gatacccaac actgtaatct atattaccaa acttacccat gtggtacagg taatcctcag
      301 tagtattcca tgtaccatag gaaccctgca attctgttgt aaatccatcc tctttttgtt
      361 tttttgtgat aacattgata acaccaccca ttgcacttga accaaataat accggttgag
      421 gtccaattaa cacttcaatt cgctcaacat ttttcatggt catgatatct gcaaccggat
      481 gtcccataat acccatcgtt gtatccatac cattgtaaag aataggaatt ccagtagttg
      541 gcccccatcc tgatctgctt ataccacgaa ttgatattgt agccgcacca cttcgtgcaa
      601 cgccataccc cattaccctg ttagacccag tatataatga tggtacctgg cttttaatga
      661 cgcttatcag gtcagtattt acactatctt ttactgtact acctgaaatt gaataatacg
      721 cacctggata caatacagta tcagacacaa ttacatcatc taactgaata acattttccc
      781 cttcctgagc aaatacaggg atatacaata gtaaaattgc tacaaaacat aagactaaac
      841 gtttcataag caccatcctt aaaaattatt taatttatag tgttacattt attataatag
      901 taatatttgt caatacaata ttttaatttt ttatttgtat agtttcttta ttttttattc
      961 aggtttattt cgatttgtta tccgctgttt agctattgat aatccttttt ctatctcact
     1021 ccgactttgc tcaccttcaa gtctgtacaa ttccatgcca ttgccatcta aaaaaagata
     1081 tactggtatc ctccataaat gatactgtga tacatacatt gcaccttcgg gattgtgtat
     1141 atccactttt tttatttcaa gtgctctgtc tatacgctga agatattcaa tagtatcatc
     1201 aattgcagag catgaatcac aattataggc actcacaact acaagcttaa ttgtttgttg
     1261 agtatctttt ttagcagaac atccaatact atgaactatc gcacatataa gtaaaactat
     1321 ataaataata attttattca tagttatcag ttactgaaca ccaatgtcgc ccatgctttc
     1381 tttttcaaga aactcaatgc gctttcgttt ttgctcatca tcccattctt tatccgataa
     1441 accttgtaaa ctgcttacct tagttttata ttcaccatca tacggtttca tcatttcccg
     1501 gtccataaat tccggatcag gcgttgttgc ttcaatgaat ctgtagccac gcaaatcccg
     1561 caacacaaaa ggtggttttg cttttttatc taaaagtact ttcccaaaaa ataaaagagg
     1621 aagccactgc ctgccagctt gtaattcccg tttttctatt gtataagcta caggttcaag
     1681 ttcattatca aaaagatttg catcaataat ataatagcct tttcttaata catccaattc
     1741 aacatcaata tagaaagaac cattttttat gtagtccctg aaatttccgg taaactttgc
     1801 cggtatggta ttttccggga aatattcaaa tggtatagcg gcatcaatta cttcatctgc
     1861 agccttaaat tcaacctgtg cccagtatgt acctgatttc ttatctgatg taaatacctg
     1921 tgcacagtta acatcagcag tatagtcacc aacattattt tttatgagcg atagctccga
     1981 cacatatttt gcagttttta tattattacc ttttttaata attgatttgg taattgttac
     2041 tggcactcta tctgagtcag gctttgtacc ctgaagtacc ataagaacaa atgtgattgg
     2101 ttcatcacca atgactcgat acttgtctgc agtaaaaaga taatagatgt cactgtcttt
     2161 ttctattggt aatggttgct cataacgacg atttggcata agtaaatcga tgttttcttt
     2221 actcagaggc ctgctgtttg ggggataaat tgctaattgc ttataatcct gaagtgcctg
     2281 ttgtggtgaa atgagtccca tctgttccag cactgttcca tagcctttta aactatcgcc
     2341 tgccgaaagt gttgaaggtt ggttattatt ttctgatttt gaaatcagca taataatcac
     2401 tataattata ataaccaaca gtattgctat atgactccct tttattttca tagtatcctc
     2461 ttaccattac tacatagttc taaatatcat actttccatt aaaaaccatt aaaaaccatt
     2521 ttattctaaa gtattcttta gtatccttta atttctttaa ttcggtataa agaacattgc
     2581 tatttgaaat tatgaaaaaa agcgcggctc tttcaaaccg cgcccgtggc aggaaaaata
     2641 atgtcaacta ccatcctaac tgatatataa atttcatttt cattccatag tgatccagat
     2701 aataaccagt acatgtatag gcaataacat gatttgtcca gtgagtacat gtacataaac
     2761 tattatagct tcctgaattt acgcatgctc ctgatgaatg ataagctact gctccatcat
     2821 cctccccagg taacaataca gatgcaccaa aaattccctt atatccacct atgtgataaa
     2881 ttgttatacc attggtatca ttatggttat acatattacg aactgtacta acacccaact
     2941 gccctgctaa gttacaggtg aaaagatcgg atatgccttt tattgctatt tcacttccgc
     3001 caccagcacc agcggttgta tatacatacg caatattcca ctgtggagta taattggcat
     3061 tgatataccc catgataaca tcacccgcac tgtagttaac gatataacac cagttgcttc
     3121 ccttgcaata tacatccaga tagttggttt taaactgact ggctgaatat gctatggtat
     3181 tgcgattgtc cacataacag ttgatagaag tatatcctga tttactagca agacgcgaag
     3241 tacctttgcc atcaagccag ttagt
//
LOCUS       DAOSZP010000216         3262 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_23240_length_3262_cov_6.235734, whole genome shotgun sequence.
ACCESSION   DAOSZP010000216 DAOSZP010000000
VERSION     DAOSZP010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3262
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23240_length_3262_cov_6.235734"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..656
                     /locus_tag="PK348_05695"
     CDS             <1..656
                     /locus_tag="PK348_05695"
                     /inference="COORDINATES: protein motif:HMM:NF018534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65740.1"
                     /translation="FAVKTGTSKDFKDNFIAGYNTQYTFAVWVGNLYNESLCDLPAVS
                     GAGIVLKDVLLYLWNAGYPFARFTMPKNIKKVKICTLSGMVADTYCPDSDYELYSGNN
                     LPCNKCNWHTKNSTIVPSQYKEWAKNKEYNVAHDAFLQIIFPGDGVVFKIEKTVRKNL
                     QAIPLKATANSNNIKWYVDNLYIGEGSERVWQLTAGHHVISAQAGNQKDVVKITVLE"
     gene            843..2936
                     /locus_tag="PK348_05700"
     CDS             843..2936
                     /locus_tag="PK348_05700"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HXK65741.1"
                     /translation="MMHKLFYPDSVAIVGLSSRPNNIPRLTLENTLRWGYSGRIFGVN
                     PKSDDEQVDGIRMYKSIEALPEIPDLVYALVPAKFVPGIVEECGQKGVQWMAIPSGGF
                     SEYSEEGNMLAQQTVANAQKYGIKFVGPNGLTVANVENGLCLPFVPILRPAFGGMSII
                     SQSGGVILTMWNVIIDENIGVAKVASIGNKLNLDEVDFLEYFGNDPATSIICMYLESI
                     TRGADLIKVAEKIDKPIVVYKANTTQIGKKAAMSHTAAMSNDEEIINMAFEKAGIIRI
                     HNYHDFFAVAKAFKLPPMHGKRVMVMSPAGGFSVITADLCENAGFEFADPGKNFYQGL
                     QKFSNAGVIQFSNPLDMGDIYDPAFTTHVVYSVMHNDNVDGAMYISQRPQMPDRKNVF
                     SQMFLSDLSKEAWGAIVSSGKPLAICLLGMAELMAQIKRSVNFPIFNSAEEMVRAMAF
                     QMKYWMRRLYHQQQEPCTLSLDTALQWIKNNNGDIGEDAMELLESIAIPVPQSGIAKS
                     EKEAVTVAKQLGYPVVCKVISRDILHKSDVGGVIVNVNGDEGVKQAYAAIQSSVLSHK
                     PDAHIEGIRISTMAPDGVDMFVGSKYDQSFGQVIIFGFGGIYVEVFKDIAYCLCPATK
                     EEIKQKLTSLKSYALLKGARGKSALDVDAYVDIIWKVSHLLASASHIKELDLNPVRVF
                     EHGCQVLDVRMRIVH"
     gene            2980..>3262
                     /locus_tag="PK348_05705"
     CDS             2980..>3262
                     /locus_tag="PK348_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011339977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HXK65742.1"
                     /translation="MVYLDNAATSFPKPDTVITAVTNCLTNYCANPGRSGHSLSIEAA
                     RKVFEVRETIAELFNAKDSRYVIFTANATMAINLVFHALLQKGDHVVVSS"
BASE COUNT         1023 a          555 c          731 g          953 t
ORIGIN      
        1 cctttgcagt gaagactggc acatcaaaag atttcaaaga taattttatt gcaggctaca
       61 acacgcagta tacctttgcg gtgtgggttg gcaatttata caatgagtca ctatgtgatc
      121 ttccagcggt atcgggagct ggcattgttt taaaggatgt gctgctgtat ctatggaatg
      181 cagggtatcc atttgcaagg tttaccatgc ctaaaaatat aaaaaaagta aaaatatgta
      241 cactgtccgg aatggttgcc gatacctatt gccctgatag cgactatgag ctgtattccg
      301 gcaataacct gccttgcaat aaatgtaact ggcacacaaa aaattcaaca attgtaccat
      361 cacagtataa ggaatgggct aagaataaag agtataatgt tgctcatgac gcttttttgc
      421 aaattatttt tcccggggat ggtgtggtct ttaaaataga aaaaacagtc cgaaaaaatt
      481 tacaggctat accgttgaag gcaaccgcta atagcaacaa tattaaatgg tatgttgata
      541 acctgtatat aggagaaggc agtgaacgtg tgtggcagtt aacagcaggg catcatgtta
      601 ttagcgcaca ggctggcaac cagaaagatg ttgtaaagat cactgtactg gaataatact
      661 cataaattca atatgtcagt agtatttaaa tcaacacaat gcaatttcaa cggctatctt
      721 ttaaaaattg cgttctggca tatactgaat ataaccataa aaaaaataaa aataattgcc
      781 atacaataac atattgttat acatggcata aatagaaaaa atatacaaaa tggagtgata
      841 cgatgatgca taaactgttt taccctgatt ccgttgctat tgtaggttta tcttcgcgtc
      901 caaacaatat tccacggctt acccttgaaa ataccttgag atggggttat agtggaagaa
      961 tttttggagt taatccaaaa agtgatgatg agcaggttga tggtatcaga atgtataaat
     1021 ccatagaagc cttgcctgaa attcctgatc tggtgtatgc actggttccg gctaaatttg
     1081 ttccaggtat tgttgaagaa tgtggtcaaa aaggcgtaca gtggatggca atccccagcg
     1141 gtggattttc tgaatacagc gaagaaggca atatgcttgc acagcaaact gttgccaatg
     1201 cacaaaaata tgggattaaa tttgtaggtc caaatggttt aacagtggct aatgttgaaa
     1261 atggattgtg cctgcccttt gtaccaatac taagacctgc atttggtgga atgtccatta
     1321 tatcacagag tggcggtgta atactaacaa tgtggaacgt tatcatagat gaaaatattg
     1381 gtgtcgctaa ggttgcaagc ataggtaata agttaaactt agatgaagtt gattttttag
     1441 agtattttgg caatgatcct gcaacgtcca ttatatgcat gtatctggaa agtatcacca
     1501 gaggtgctga tttaattaaa gttgcagaaa agattgataa accaatcgtt gtatataagg
     1561 caaacacaac gcagataggg aagaaggcgg caatgagtca tactgctgca atgagcaatg
     1621 atgaagaaat aatcaacatg gcatttgaaa aggctggtat tatacggatt cataattacc
     1681 atgatttttt tgctgtagct aaagcattta aacttcctcc aatgcatgga aagcgggtta
     1741 tggtcatgag ccctgcaggt ggttttagtg ttattaccgc tgatctatgc gagaatgccg
     1801 gatttgaatt tgctgatccg gggaagaatt tttatcaggg tttgcaaaaa ttttccaatg
     1861 cgggtgttat acaattttcc aatcctttag atatgggcga catctatgac cctgccttta
     1921 ccactcatgt tgtgtattcg gttatgcaca atgataatgt tgatggcgct atgtatataa
     1981 gccagcgtcc acagatgccc gacagaaaaa atgtcttttc gcagatgttt ttatcagacc
     2041 tttccaaaga agcatggggt gcaattgttt cttcgggaaa accgcttgca atttgcctgt
     2101 taggtatggc agaattaatg gcacagataa aacgttcggt gaattttcca atatttaaca
     2161 gtgctgagga gatggttcgt gccatggcat tccagatgaa atactggatg cgacgtttat
     2221 accatcaaca gcaagaacca tgtacactga gtcttgatac agcgttacaa tggataaaaa
     2281 ataacaatgg cgatattggt gaagatgcca tggaactgct tgagtcaata gctattcccg
     2341 tgccacagtc agggatagca aagtcggaaa aggaagcggt aacagtagca aaacaacttg
     2401 gctatcctgt tgtatgcaag gtcatttccc gcgatatttt acacaagtca gatgttggtg
     2461 gggtgatagt taatgtgaat ggagatgaag gtgtaaagca ggcttatgca gcaatacaat
     2521 ccagtgtact atcgcataaa cctgatgcac acattgaagg tatacgcatt tcaacaatgg
     2581 cacctgatgg agtggatatg tttgtgggga gcaaatatga tcagtcattt ggacaggtta
     2641 ttatctttgg ttttggtggc atatatgttg aggtgtttaa agacatagcc tattgtttat
     2701 gcccggctac aaaggaagaa attaagcaaa agcttacttc attaaaatcg tatgcactgc
     2761 ttaaaggtgc gcgtggcaaa agtgcactgg atgttgatgc ctatgttgac attatatgga
     2821 aggtatcaca tcttttagcc agtgcatccc atattaaaga gctggacctt aatcctgtgc
     2881 gggtgtttga acacggctgt caggttttag atgtgaggat gagaatagtt cattaaattt
     2941 taatttgcag gttgttgcag cactgctata cttgtggcta tggtatatct tgataatgca
     3001 gcaacaagtt ttcccaaacc ggatacggta ataacggctg taaccaattg cttaacaaac
     3061 tactgtgcca atccaggccg ttcaggacat tcactttcca ttgaagcagc gcggaaagtg
     3121 tttgaagtgc gtgaaaccat agctgaattg tttaatgcaa aagattcacg gtatgtcatc
     3181 tttactgcca atgccacaat ggcaataaat ctggtttttc atgcactttt gcaaaaaggc
     3241 gatcatgtcg tcgtctcatc aa
//
LOCUS       DAOSZP010000217         3256 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_23309_length_3256_cov_3.187441, whole genome shotgun sequence.
ACCESSION   DAOSZP010000217 DAOSZP010000000
VERSION     DAOSZP010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3256
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23309_length_3256_cov_3.187441"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..932
                     /locus_tag="PK348_05710"
     CDS             <1..932
                     /locus_tag="PK348_05710"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HXK65743.1"
                     /translation="VGQGEYIVFEADESDGSFLKLMPTIGVVTNIDADHLDHYKYFEG
                     IKEAFVTYMNNIPFYGYSVVCIDDPVVFELLPKIERPFVTYGFNPKADFRAGDVKLLN
                     GSTRYSCYYKEEKLGEIVLTLLGKHNVINSLAVVAVALELGLSFEKIADAIAAFKGVG
                     RRLEKIGEARGILVVDDYGHHPTEIRATLEAVKQLGRRVVVIFQPHRYSRTQYLYDEF
                     GQAFAMADELLLTEIYPAGEEPIEGVSSQLIQQAVKKHEGRDVEIIQHLDDVPEYVCR
                     IARKGDVILTLGAGDIYKVGPKILSVIEGKGIR"
     gene            929..1615
                     /locus_tag="PK348_05715"
     CDS             929..1615
                     /locus_tag="PK348_05715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65744.1"
                     /translation="MKVAFYIWIMILFCGVIPTVTYADVVQVTHVRFQGLKYVTQHEI
                     LQHGVFMKDGKIFVDVAVLNEELKKNIMIESYNITIEKNICTIQVVEKDILAIILYDK
                     GDSLVSVEVDANLNIIPTSRVYAYDRPIIKINQAEVINNKLSPRIIRILIILKNMIQN
                     NAIVSRISVINCTDPEVSYVQFHGFTSRCIWDETYNGLSLLDAVVGVYDSKKAYPGNA
                     MVTQKRMVVW"
     gene            1621..2862
                     /gene="ftsA"
                     /locus_tag="PK348_05720"
     CDS             1621..2862
                     /gene="ftsA"
                     /locus_tag="PK348_05720"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HXK65745.1"
                     /translation="MKEDTILDNIVVGLDIGTTKTCAVIGFLNENKQVEVVGVGVAPS
                     RGLKNGVIVNIDNTVSSIVKAIEDAELMAGCEVSSVFVGVTGQHIKGENSRGVVAVAN
                     RSRTITPLEMKRVIEAAQAIVIPMDREIIHVLSKEFAVDDQTGIKDPIGMTGVRLEAE
                     VHIITGSTTSIQNMVKSINKAGFTCNDIVFSPLASAEATLSRDEKDLGVALVDIGGGT
                     TDIIIFIEGGVAYSAVLGIGGIHVTNDISIGLRTPIDSAEVIKKKYGCAVVDLVDASE
                     TIEVPSVGGRAPRRLFRHELAQIIEPRMIEIMEMIDNELVKSGKKEILAAGVVLTGGG
                     SMIEGTVEAAERVFGMPVRVGVPENIAGLKDVVSTPVYANGVGLLKYGAKMSQVRQTR
                     KTKSAFTSFKDKLKHIFNDYL"
     gene            2937..>3256
                     /locus_tag="PK348_05725"
     CDS             2937..>3256
                     /locus_tag="PK348_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HXK65746.1"
                     /translation="MFRFEEEPQMNTVIKVVGVGGAGNNAVNRMIATGLEGVDYIVVN
                     TDAQQLKASLAQNKIQIGGKLTRGLGAGADPSIGREAALEDRDKLEKALKGADMVFIT
                     AGMGG"
BASE COUNT         1020 a          461 c          824 g          951 t
ORIGIN      
        1 gggtaggcca gggagagtat atagtatttg aagcagatga atcggatggg tcatttctta
       61 aacttatgcc aactattgga gttgttacca atattgatgc tgaccatctg gatcactata
      121 aatattttga agggattaaa gaagcttttg ttacttatat gaataatata ccattttatg
      181 gttattcagt agtgtgtatt gatgatcctg tagtttttga acttttacca aaaattgaac
      241 gcccatttgt aacgtatgga tttaatccaa aggctgattt tagggcgggt gatgttaaac
      301 tactcaacgg ttcaacacgg tatagttgtt attacaaaga agaaaagtta ggggagatag
      361 ttcttacctt acttggcaaa cataatgtta ttaattcact ggctgttgtt gctgttgccc
      421 tggaactggg attatcattt gaaaagattg ctgatgctat tgccgcattt aaaggtgttg
      481 gaaggcgctt agagaaaatt ggagaagccc ggggcattct ggtagtagat gactatggtc
      541 accacccaac tgagatacgg gcaacattgg aggctgtaaa acagttgggg aggcgggttg
      601 tagttatttt ccagccacac cgttattcta ggacacagta tctgtatgat gaatttggac
      661 aggcatttgc aatggctgat gagttgttgt taaccgaaat ttatccagct ggtgaggaac
      721 caattgaagg tgtcagcagc cagcttatac agcaggcggt taagaagcat gaaggaagag
      781 atgtggaaat tattcagcac ttggatgatg ttccggaata tgtatgcagg atagccagaa
      841 aaggtgatgt aatcctgacg ttaggtgctg gtgatattta taaagttggg ccaaagatac
      901 tttcagtaat agagggtaaa gggataaggt gaaggttgct ttttacatct ggataatgat
      961 actgttttgt ggagttatac ccaccgtaac gtacgcagat gtggtgcagg taactcatgt
     1021 gaggtttcag ggattgaaat atgttaccca gcatgaaata ttgcaacatg gcgtttttat
     1081 gaaagatgga aaaattttcg tagatgttgc tgtgttgaat gaagagttga agaaaaatat
     1141 catgattgaa tcgtataata ttactatcga aaagaacata tgtacaatac aggttgttga
     1201 aaaggatatc ctggctatta ttctctatga taaaggagat tctcttgttt cagttgaggt
     1261 ggatgcaaat ctgaatatta tcccaaccag tagagtttat gcctatgacc gaccgataat
     1321 taaaataaac caggcagagg ttattaataa taaactatcg ccaagaataa tacgtattct
     1381 tattattttg aaaaacatga tacaaaacaa tgccattgta agcagaattt cggtaattaa
     1441 ttgcacagat cccgaagtat cgtatgtgca atttcatggt tttacatcac gatgtatatg
     1501 ggatgaaaca tacaatggcc tttcattact tgatgctgtt gtaggagtat atgatagtaa
     1561 aaaagcttat cctggtaatg ccatggtgac acaaaaacga atggtggtat ggtgacagat
     1621 atgaaagaag ataccatatt agataacatt gttgtagggc tggatattgg aacaacaaaa
     1681 acctgtgcag tcattggctt tttgaatgaa aataaacagg ttgaagttgt aggtgttggg
     1741 gttgcgcctt cacgaggttt aaaaaatggc gttatagtaa atattgataa taccgtttct
     1801 tcaattgtca aagcgataga agatgcagag ttaatggctg gttgtgaagt aagttcggta
     1861 tttgttggtg ttaccgggca gcatataaaa ggtgaaaact caagaggtgt ggttgctgtt
     1921 gcaaaccgaa gccgtacaat cacgccactt gaaatgaaac gggttattga agctgcacag
     1981 gctatcgtta ttcccatgga tagagagata atccatgttc tatccaaaga atttgctgtt
     2041 gatgatcaga caggtataaa ggatcctata ggaatgactg gtgtaaggct tgaagctgaa
     2101 gtgcatatta ttactggctc tactacaagc attcagaata tggtgaaatc tattaataaa
     2161 gctggcttca catgtaatga catagtattt tcgcctctgg cttctgctga agcaacgttg
     2221 agccgtgacg aaaaggattt aggtgtggcg cttgtggata taggtggtgg cactacggat
     2281 atcatcattt ttatagaagg tggtgtggca tattcggcag tattggggat aggcggcata
     2341 catgttacta atgatatatc cataggatta aggacgccaa ttgattctgc agaagttatc
     2401 aagaagaaat atggatgcgc agtggtagat ctggttgatg cttctgagac aattgaggtg
     2461 ccatcggttg gtggaagggc accgcggaga ttattcaggc atgaactggc acagattatt
     2521 gaaccgcgga tgattgaaat aatggaaatg attgataatg aactggtaaa aagtggtaaa
     2581 aaggaaatac tggcagctgg tgtggtgttg actggtggcg gcagcatgat agaaggtacc
     2641 gtggaggcag ccgaaagagt atttggaatg ccagtacgtg ttggcgtccc agagaatatt
     2701 gccggattga aagatgttgt gtcaactccc gtgtatgcca atggtgttgg attattgaaa
     2761 tatggtgcta agatgagcca ggtgaggcaa acacgcaaaa ctaaaagtgc ttttacaagt
     2821 tttaaggata aactcaagca tatttttaat gattatttat aatacagtcg ttgcagttga
     2881 attttttatt aatttttttt aaaaaataat gcgacataat taaaaaggag gaaataatgt
     2941 ttagatttga agaagaacca caaatgaata ctgttatcaa ggttgttggc gttggaggtg
     3001 caggcaacaa tgcagtaaat aggatgattg caacaggcct tgaaggagtg gattatattg
     3061 ttgtgaacac ggatgcccag caattgaagg catcgctggc tcaaaacaaa atccagatag
     3121 gtggtaagct gaccagagga ttgggagcag gtgctgatcc atcaattggt agggaagctg
     3181 cacttgaaga cagagacaaa ctggaaaagg ccttgaaagg ggctgatatg gtattcatca
     3241 ctgctggaat gggtgg
//
LOCUS       DAOSZP010000218         3242 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_23462_length_3242_cov_2.484782, whole genome shotgun sequence.
ACCESSION   DAOSZP010000218 DAOSZP010000000
VERSION     DAOSZP010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3242
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23462_length_3242_cov_2.484782"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(60..827)
                     /locus_tag="PK348_05730"
     CDS             complement(60..827)
                     /locus_tag="PK348_05730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65747.1"
                     /translation="MKKNFYWLFIMACVSLISFTSCDPKDPIDDPDETKEMRIKTMTF
                     EYDWGSGPGSTSYAYTYDAQGRVTKVVETGEDWTEEFNLDWSQPGKVNLIREEANKNR
                     TWILNAAGYVSKIENIWGDGGDVIFEYDNNGLMSKIYEDYGTPELKSLFTIINGNITS
                     FTRGDRVKNFTFSTGENIGKIYQVFNDTYIADWQAHTGLFGKACKNLNTKVQWSNSEN
                     FTAISFEFYDDGRVKKVIRSGSDWYENYVYTYEEFNN"
     gene            complement(876..1883)
                     /locus_tag="PK348_05735"
     CDS             complement(876..1883)
                     /locus_tag="PK348_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HXK65748.1"
                     /translation="MFIVNSYSLAVLLCFITMFCWGSWGNTQKLAAKTWRYELFYWDY
                     VIGILLFSVILGFTLGSFGEGGRSFIPDLKQAELSNIGSALLGGVVFNAANILLAASV
                     SLAGLAVAFPLGVGIALVLGVFVNYFSAPKGDPVLLFIGVVLVMLAVIFNGLAAGKIK
                     KDDAAKSNRKGIWLAVLAGVLMAFFYRFVAAAMDLNNFESPAGGMMTPYSAFFIFSIG
                     IFISNFLFNTLVMKKPFQGEPVTYKAYFNGNMKTHLVGVLGGLIWALGTALSYIAAGK
                     AGAAISYALGQGAPMIAAIWGVFVWKEFKGSNKQTNVLLALMFLLFISGLAFIIISGG
                     N"
     gene            complement(1912..>3242)
                     /locus_tag="PK348_05740"
     CDS             complement(1912..>3242)
                     /locus_tag="PK348_05740"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HXK65749.1"
                     /translation="SILDCMTDKKEIKPFICVMPQGFKSYYTDSYDGTFNYQQMFIDE
                     LVPYIDSVYRTIPDASQRAVTGYSMGGFGAFVLPVKYPDVFSISIPLSASLRTDEQYI
                     NEHPDGWNEQWGKIFGGVGESGDARITDYYKANSPFYLIESKSVDELKKVAFYIENGD
                     KENTLCRSNEFLHIAMLKKGIPHLYNVKEGGHEFSFWRNALPEVFRFTDACFRKVEYL
                     VDAKEIKTKHALPENMTVQEEHEGEISYRVIYPENLKESSRLYPVVYFVADLTKEEQA
                     GLMNLYQGNMQNAGFPPVIFCFVPSKNAGKLSDYIIPRMEKTGRARPGWHFRSLWVYH
                     CAGDAVVKQAFDSLQFTVGVLTTANIRLKESEIAALMTGKPNIRDRMWSYIDTPPTGE
                     SYPGNGYMHVYLRENGYKHEYRVRTHKNTFEFLKSGFMPAMKYISKKFHN"
BASE COUNT         1019 a          732 c          523 g          968 t
ORIGIN      
        1 cgctacgttt taccaagtca acaattcttt aatacaaaaa tgggcttttt aaggttcgac
       61 taattattaa attcctcata tgtataaaca taattttcat accaatcact acccgagcgg
      121 attacttttt tcacccttcc atcatcataa aattcaaaag aaattgcagt aaagttttca
      181 ctgtttgacc attgaacttt agtattcagg ttcttacatg cttttccgaa taaacctgta
      241 tgggcttgcc aatcagcgat ataagtgtca ttaaaaacct ggtaaatctt gccaatattt
      301 tcacctgttg aaaatgtaaa atttttgact ctatcaccac gtgtaaaaga tgttatatta
      361 ccattaataa tcgtaaataa agattttagc tctggggttc cataatcttc atatattttg
      421 gacattaatc cgttgttatc atattcaaag attacatcac caccgtctcc ccagatattt
      481 tcaatttttg aaacataacc ggcagcattc agaatccagg ttctgttttt gttggcttcc
      541 tcacggataa gatttacttt tccgggctga ctccaatcca ggttaaactc ttcagtccaa
      601 tcttctcccg tttccacaac ttttgttacg cgaccctgag catcgtaggt gtaagcgtaa
      661 ctggtactgc ctggaccact tccccaatca tactcaaatg tcatcgtttt aatacgcatt
      721 tcttttgttt cgtccggatc atcaatcgga tctttcggat cacaagaagt aaaactaatt
      781 aaactaacac aggccatgat aaacagccaa taaaaattct ttttcatgat ttcaaattat
      841 ttaaagttca acaatagaga tatttataat taaaattaat ttccacctga tataatgata
      901 aatgcaagtc ctgaaataaa caacagaaac atcaatgcta acaagacatt cgtttgttta
      961 ttagaacctt taaattcctt ccacacgaaa acaccccaga ttgcagctat cataggagct
     1021 ccctgtccca atgcatacga gatagccgct ccggctttac ctgctgcaat atagctcaat
     1081 gctgtaccca gcgcccagat cagacctccc aacacaccaa ccaaatgggt tttcatgtta
     1141 ccgttgaaat aggctttata ggtcacaggt tctccctgga aaggtttttt catcaccagt
     1201 gtattaaata aaaagttgct gataaaaata cctatcgaga aaataaagaa tgctgaataa
     1261 ggagtcatca tgcctcctgc cggtgattcg aaattattca aatccatcgc agcagcgaca
     1321 aaacgataga agaaagccat caacacaccg gctaacaccg ccagccagat accttttctg
     1381 tttgattttg ccgcgtcatc ctttttaatt ttgccggcag ccagaccatt gaaaataaca
     1441 gcaagcataa ccagcactac cccgataaac agcaatacag gatcgccttt cggtgcagaa
     1501 aaatagttta caaaaacgcc taacaccaac gctattccca cacccaatgg aaatgcaaca
     1561 gctaaccctg ctaaagacac cgaagcagca aggagaatat ttgcagcatt aaatactact
     1621 ccccccagta atgcactacc gatattagaa agttcagcct gtttcaggtc aggaataaaa
     1681 gaacgtccac cttccccgaa acttccaagt gtaaaaccga gaatgacact aaagagtaat
     1741 attcctatta cataatccca gtaaaacaat tcgtaacgcc aggttttggc tgcaagtttc
     1801 tgcgtgtttc cccacgaacc ccagcaaaac atggtaataa agcagagtaa tactgcaagt
     1861 gaataactgt ttacaatgaa cataataatg tgtttttagg taaataaata cttaattatg
     1921 aaatttcttg ctgatatact tcattgccgg cataaaaccc gactttaaaa attcaaatgt
     1981 atttttatgt gttctgacac ggtattcatg tttataaccg ttctctctga gataaacatg
     2041 catataacca ttacccggat aggattcgcc tgtgggaggt gtatcgatat acgaccacat
     2101 tctatccctt atattgggtt tccccgtcat caaagcggct atttcactct ctttcagcct
     2161 tatattggcg gttgtcaata cccctacagt aaattgtaat gaatcgaatg cttgttttac
     2221 cacagcatcg ccggcacaat gataaaccca caaagaacga aagtgccaac ccgggcgtgc
     2281 acgacctgtt ttttccatcc gggggatgat ataatccgat aatttaccgg cattttttga
     2341 aggaacaaaa caaaagatta ccgggggaaa acctgcattt tgcatattac cctgatacaa
     2401 attcattaac cctgcttgtt cttccttcgt taaatctgcc acaaaataaa cgacaggata
     2461 caaacgggat gattctttca ggttttccgg atatatcacc ctgtatgaaa tttcaccttc
     2521 atgctcttcc tgtacagtca tattttcagg taaagcatgt ttcgttttta tttcttttgc
     2581 atcaactagg tattcaacct ttctgaaaca ggcatcggta aaccggaaca cttccggcag
     2641 agcatttctc cagaaactga attcatgtcc gccttctttc acattataca gatgcggaat
     2701 tccctttttc aacattgcta tatgcagaaa ttcgttgcta cggcataaag tattttcttt
     2761 gtctccgttc tctatgtaaa aagccacttt tttaagttca tcaacacttt tactttcaat
     2821 cagataaaag ggtgaattag ctttataata atcagttatg cgtgcatcac ccgactctcc
     2881 aactcctccg aaaatcttac cccattgttc attccatccg tcaggatgtt cattgatata
     2941 ctgttcgtct gtacgaaggg aggcactaag tggaatgctg atactaaaca catcaggata
     3001 tttcaccggc aatacaaatg ctccgaaacc acccattgaa tatccagtca ctgcgcgttg
     3061 cgatgcgtca ggaatggttc tgtaaacaga atcaatgtag ggaacaagtt catcaataaa
     3121 catttgctga taattaaatg tgccatcata agaatcggtg tagtacgact tgaaaccctg
     3181 tggcatgaca caaataaaag gtttgatttc ttttttatca gtcatgcaat cgaggatgga
     3241 ag
//
LOCUS       DAOSZP010000219         3237 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_23504_length_3237_cov_3.475173, whole genome shotgun sequence.
ACCESSION   DAOSZP010000219 DAOSZP010000000
VERSION     DAOSZP010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3237
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23504_length_3237_cov_3.475173"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..970)
                     /locus_tag="PK348_05745"
     CDS             complement(<1..970)
                     /locus_tag="PK348_05745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65750.1"
                     /translation="MKLVRSIVSKIGFAESKQTDFNEGIHFIFGKNNSGKTLISKSFI
                     DTIYPQNPSLIDSEAWDTMFATFTLECGQSKVEIQRKGNKEVKIFEITSNGNTQKEDT
                     YPLSVNPGSLSVNCLSKLHGLFNKDTFAILCYIPSPLDTQYYSKDFSDSMKSVLLLDR
                     SLYYYAFSRLFTDTINKTGNYQAHVLAEIQNKIKHCKTKLDLLQLKEQKQKKHRAEIQ
                     EVNERITSYTNDINELQKKIEACNRYMDTLVAIDNVKKQIEDVNGQIEALEEKLETIE
                     KYTKLIASKYPQFKDFSSEKRNNIHAMQNIYRQIQAISEEIETIIFG"
     gene            complement(967..2028)
                     /locus_tag="PK348_05750"
     CDS             complement(967..2028)
                     /locus_tag="PK348_05750"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HXK65751.1"
                     /translation="MISILLMSDIHFGSDKALPYIDDSVRFATFQKICMIARQHSIFI
                     IAGDLFEGTIAQNPYYSSIKELLQSLVQEGVKIIYIPGEAELEDNSNIDVIHEIPSTV
                     TLFGRSKNYFDLVLDKEIVYCYGGDSDIASITKASQEGFHIGIFHIDLEENNHNAIDS
                     HVISVKLLKSMPLDFYALGHHHNFSMYKYKNKIIGAYPGSPEAVAPGETGDRYVLSIT
                     VMNNEIHQIKRITVNSLRIESLTIQCNSIKSNDELHHILKPYNKPSIVLHCTLEGVRS
                     FDSNHIVALCDRCAGVIFQDNSKLSGEFICAKYKDEKTLKGYFFTELQKNFDNVAGDI
                     MLDIINDIENTCSTYKGDL"
     assembly_gap    2212..2221
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2476..>3237
                     /locus_tag="PK348_05755"
     CDS             2476..>3237
                     /locus_tag="PK348_05755"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HXK65752.1"
                     /translation="MQGINSTPTVMEIITTLPQERTGIDRIVGNNKTIISLKNKIKQI
                     AGSKATVMILGESGTGKELIARAIHEESQRKHNQFIPVDCSTLTETIIESELFGHIKG
                     AFTGADKNKKGLFEEANGGTIFLDEIGNLQPHIQSKLLRFLQEREIKPVGSSKIVKVD
                     VRIISATNINVDEEISQGNFREDLYYRLSAIELHIPPLRERRDDIPVLAEHFIHMYSH
                     DLQKTIIGIRDDALELLSNREWKGNVRELQNVIEHA"
BASE COUNT         1064 a          585 c          525 g         1053 t
ORIGIN      
        1 gtccaaagat aatagtttct atctcttcag aaatagcctg tatctgccgg taaatgtttt
       61 gcattgcatg aatattgttc ctcttttccg aggaaaagtc tttaaactgg ggatatttgc
      121 tggctatcag ttttgtatat ttttctatag tttctaattt ttcttcaaga gcttctatct
      181 gaccattaac atcctctatc tgctttttaa cattatcaat agcaaccagt gtatccatat
      241 atctattaca ggcttctatc tttttctgaa gttcattaat gtcattggta tatgacgtaa
      301 ttctttcatt aacttcctgt atttctgcac gatgtttttt ctgtttctgt tccttaagtt
      361 gcagtaaatc aagttttgtt ttgcaatgtt ttattttgtt ctgaatctct gccagcacat
      421 gagcctgata attgcctgtt ttgtttatcg tatcagtaaa caacctggaa aacgcataat
      481 aatataatga tctatctaac aaaagaactg atttcatgct gtcagaaaaa tctttgctgt
      541 agtattgtgt atcaagcggt gaagggatat aacaaaggat tgcaaatgta tctttattaa
      601 aaagtccgtg caacttagaa agacagttaa cggacaaact tcccggattg acactcaaag
      661 ggtatgtatc ctctttttga gtattgccat ttgatgtaat ttcaaatatt tttacttcct
      721 tattgccttt tctctgaatc tcaacttttg actggccaca ttcaagcgta aatgtggcaa
      781 acattgtatc ccatgcttcg ctatcaatta atgatggatt ttgaggatag attgtatcaa
      841 taaagctttt tgaaatgagt gttttgccgg aattattttt gccaaagata aaatgaatcc
      901 cctcattaaa atctgtttgt ttgctttctg cgaatccaat ttttgatact atactccgta
      961 ctaacttcac agatcacctt tatatgttga acaggtattt tcaatatcat tgataatatc
     1021 cagcattata tccccagcaa cattgtcaaa atttttctgc aattcagtaa aaaaataccc
     1081 tttgagtgtt ttctcatctt tatacttagc acatataaat tcacctgaaa gctttgaatt
     1141 gtcctggaat attacaccag cacatctgtc gcataaagcc acaatatgat tactgtcaaa
     1201 tgaacggact ccttccagtg tgcagtgtaa aacaatggaa ggtttgttat atggcttaag
     1261 tatatgatgc aattcatcat ttgacttaat actattgcac tgtatagtca gcgactctat
     1321 ccgtaatgaa tttactgtta tacgttttat ctgatgaatt tcattattca taacagtaat
     1381 tgaaaggaca tatctgtcac cggtttcacc aggtgctaca gcttccgggc tcccgggata
     1441 tgctccaata attttatttt tatacttata cattgaaaaa ttatggtgat gtcccagtgc
     1501 ataaaagtct aatggcatcg attttaacag tttaactgaa atcacatggg aatcaatggc
     1561 attgtgatta ttttcttcaa ggtcaatatg aaaaatacca atatggaacc cttcctggct
     1621 tgcctttgta atactcgcaa tgtcactatc ccctccatag caatacacaa tctccttatc
     1681 aagaacaaga tcaaaataat ttttggagcg gccaaataac gtaaccgtag aaggaatttc
     1741 atgtataaca tctatattgc tgttatcctc cagttcagct tcgccaggaa tgtatataat
     1801 ttttacgcct tcctgtacta atgactgcaa cagttcttta atggaactat aatagggatt
     1861 ctgtgcgata gttccttcaa ataaatcacc tgcaataata aaaatcgaat gctgcctggc
     1921 aatcatacag atcttctgga acgtggcaaa tctgacactg tcatcaatat atggcagtgc
     1981 cttatctgaa ccaaaatgga tgtcagacat caacaaaatc gaaatcattg tttatatttc
     2041 actttctacg catgtaatta ttttttaagt aattattcct tcagaatatt ttattaataa
     2101 atacctgcat accaccgcaa caagcagcgg aatatacgtt acataatttt ttaaaaaaat
     2161 tatgaattat agttgcaaat aacgacactt tggtcaaaca taatttaaaa annnnnnnnn
     2221 ngattactgg taaaaatgat gtagcccctt tatataaggg atattaacag taatataaaa
     2281 aaattagtct ggatttgaaa tgcaattcat gtaactactt ttatagtgta tttaagctat
     2341 tgtattcatt ttacatggta ttgaacagaa tatataaaat taaaactgaa agcatagtaa
     2401 taaagcaata agcatactgt aacagtggtg tatttttgcc acagaaacaa tataaaaatc
     2461 ttacagcaag ggaaaatgca agggattaat tcaacaccaa ctgtaatgga aattataacc
     2521 actttaccac aggaaagaac aggtattgat agaatagttg gcaacaataa aactatcatt
     2581 tctcttaaaa acaaaataaa gcagatagct ggctcaaaag ctactgtcat gatattgggc
     2641 gaaagcggta ccggcaaaga gcttattgcc cgtgccattc atgaagaaag ccagagaaaa
     2701 cataatcagt ttatacctgt tgactgcagc acacttactg aaaccatcat agaatctgaa
     2761 ctatttggcc atattaaagg agcatttacc ggcgcagata aaaataaaaa aggactattt
     2821 gaagaagcca acggtggtac tatatttctt gatgaaattg gtaaccttca accacatata
     2881 cagagcaagc tcttacgttt tttacaggag cgtgaaatca aaccggtagg atcaagtaaa
     2941 atagtaaaag ttgacgtacg gattataagc gcaacaaaca taaacgtgga tgaagaaatt
     3001 tcacagggga atttcaggga agacctatac tatcgtctct cagcaattga acttcacata
     3061 ccaccattac gtgagcgccg tgatgacata cctgtgcttg ctgagcactt tatacatatg
     3121 tactcccatg acctccaaaa aaccattatc ggcatacgtg atgatgcatt ggaactgctt
     3181 tccaatagag aatggaaagg caacgtccgt gaactacaga atgttattga acatgca
//
LOCUS       DAOSZP010000220         3230 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_23569_length_3230_cov_3.039055, whole genome shotgun sequence.
ACCESSION   DAOSZP010000220 DAOSZP010000000
VERSION     DAOSZP010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3230
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23569_length_3230_cov_3.039055"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1849
                     /locus_tag="PK348_05760"
     CDS             <1..1849
                     /locus_tag="PK348_05760"
                     /inference="COORDINATES: protein motif:HMM:NF027585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4914 family protein"
                     /protein_id="HXK65753.1"
                     /translation="KALLQVTNVTVAHTVDDLFELSLKFGNDGVQKVVYDVPGKGEYC
                     EAVVYKVKNGIAVNYTEAYMRRRDPDSMVIGDDLPTDKITYTQRSGKDFNELKKETFD
                     WLQKQDLAVFFFKAGQLDGCYGMAIAPANAGFFCLGLGILQGIIDSRQIEGTIHVKCI
                     LYIAPVFRHTHFNGKQIVVHYRSENLHEIYSYNLYPGPSAKKGVYSSLLDFGEKEGWV
                     TNHCAVVQVITPYGNKTTIMHEGASGSGKSETNEHIHRDVDGTIKLGVNIVTGEVLSL
                     VLPRGCFLRPVVDDMGLCHPSFQKDDGYLYLKDAEAGWFIRVNHIEQYGTDPDIESLS
                     IHPKVPLMFLNIDAQPFSTALLWEHIEDEPGKSCPNPRFLVPRNALPDIVNKPVQVHI
                     RSFGVRTPPSTKEKPDYGIIGLFHILPPAIAWLWRLVSPRGHDNPSIVKTGGLESEGV
                     GSFWPFATGKKVNFANLLLEQIIASPKVKYVLVPNQYIGAWQVGFNPQWIMREYLARR
                     GGVKFFADELSKSRCSLLGFSLNKLVIEGVEIEKFLLRPELQSEVGEDAYDRGAQILV
                     DFFKKELMDYYNDPNLLPLGKQIIECFLSDGTIEEYMQLIKDEGLIMED"
     gene            1850..2398
                     /locus_tag="PK348_05765"
     CDS             1850..2398
                     /locus_tag="PK348_05765"
                     /inference="COORDINATES: protein motif:HMM:TIGR01662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HXK65754.1"
                     /translation="MALQKYPSLFLDRDGTLIKDVGYISNPGRVVFYTCTFDALKEAQ
                     QFFRLFIITNQPGIAKGIVTLDEAITVNTYIQSVFADKGITIESIYMCPHAKEDNCHC
                     RKPKTYFVDQAVAYYNLDVQHSFVIGDHPSDILLAKNAGMQGIYVLTGHGKKHKKEID
                     NCVIVKKNLQYAVDYILKRVQL"
     gene            2395..3195
                     /locus_tag="PK348_05770"
     CDS             2395..3195
                     /locus_tag="PK348_05770"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HXK65755.1"
                     /translation="MKKPFFEKPARIFAHRGYSKEYPENTALAFEKAIALGADVIETD
                     AHLTRDGHVVIVHDEELSHITDGKGNVGQLTLHEIKQLDAAYHFSLDGETYPYRGKGF
                     TIMTLEEMLDAFPEQRFNVDLKDNTIVLAEKFCDIVLSHKSEHRVLGASQHGKNLQYI
                     RKRIPTMATSFGLWGVLGFYFLFRSGLLGLFKHFPGDALQIPEYIGPSHLANGALIRM
                     AHERGIQVDVWTVNQERDMRRLLDVNVDGIVTDDVPLLQKVLQDYPLK"
BASE COUNT         1008 a          508 c          743 g          971 t
ORIGIN      
        1 aaaagcgtta ttacaggtta caaatgtaac ggtagcgcat acagttgatg atttatttga
       61 gttatcactc aaatttggga atgatggtgt tcagaaagtt gtatatgatg tgccaggcaa
      121 aggggaatac tgtgaggccg ttgtttataa ggtaaaaaat gggatagctg ttaattatac
      181 tgaagcatac atgcgcagac gcgatcccga tagcatggtt attggagatg acctgccaac
      241 tgataagata acctatacac aaagatctgg gaaagatttt aatgaactta aaaaagaaac
      301 atttgattgg ttacaaaagc aggacttagc agtgtttttc ttcaaagcag ggcaacttga
      361 tgggtgttat ggtatggcaa tagcacctgc aaatgctggt ttcttttgtt taggcctggg
      421 gatattacag ggaatcattg attcaagaca aattgaaggc accatacatg ttaaatgtat
      481 actctatata gcacctgttt tcaggcatac ccatttcaat gggaaacaga tagttgttca
      541 ctataggagt gaaaatctgc atgaaatata cagttataat ctgtatcccg ggccaagtgc
      601 aaagaaaggt gtatattcat cgttgcttga ttttggtgag aaggaaggtt gggtaacaaa
      661 tcactgtgct gtggtgcagg ttattacccc gtatggcaat aagacaacca taatgcatga
      721 aggtgccagt ggaagtggta aaagtgaaac caatgaacat attcatcgag atgtggatgg
      781 taccataaag cttggtgtga atattgtgac tggcgaagtg ttatcactgg tactgccacg
      841 ggggtgtttt ttgcggccag ttgttgatga tatgggatta tgccatccat catttcaaaa
      901 agatgatggt tatttatacc ttaaagatgc tgaagcagga tggtttatac gggttaatca
      961 tattgaacag tatggcactg atcctgatat tgaatcgctt tcaatacatc caaaggtgcc
     1021 attaatgttt ttgaatatag atgcgcagcc attcagtact gctttattat gggaacacat
     1081 tgaagatgag ccgggtaaat catgtcccaa tcctcgtttt ctggttccac gcaatgcatt
     1141 gccggacatt gttaataaac ctgttcaggt tcatataaga agttttggtg tcaggactcc
     1201 tccttcaaca aaagaaaaac ctgattatgg aataataggt ttattccata tattaccacc
     1261 ggcaatagcc tggttgtgga ggcttgtgtc acctcgtggc catgataatc caagtattgt
     1321 aaaaactgga gggcttgaat ccgaaggggt tggttctttc tggccttttg caactggtaa
     1381 aaaagttaat tttgcaaatt tgctacttga gcagataatt gccagtccaa aggtaaaata
     1441 tgtgctggta cccaatcagt atattggtgc ctggcaagtt ggatttaacc cacaatggat
     1501 tatgcgtgaa tatttagcaa gaagaggtgg tgtaaaattt tttgctgatg aattgtctaa
     1561 atcacgttgt tcattgttgg gattttcatt aaataagctt gttatagaag gagtagaaat
     1621 tgagaagttt cttttaaggc ctgagcttca aagtgaagtt ggtgaagatg cgtatgacag
     1681 gggagctcag atactggttg attttttcaa gaaggaactt atggattact acaatgatcc
     1741 caatcttctt cccttaggca aacagatcat tgagtgtttt ttgtctgatg gaacaattga
     1801 agagtatatg cagctcatca aagatgaagg tttaataatg gaagattaaa tggcattgca
     1861 aaaatatcct tcattatttc ttgatagaga tggcacactc ataaaggatg tggggtatat
     1921 aagtaatccc gggcgtgtag ttttttatac atgtacgttt gatgctttga aggaagcgca
     1981 gcagtttttc aggctgttta tcataaccaa ccagcctggt attgcaaaag gaattgtaac
     2041 cttagatgag gctattacag taaatacgta tattcaaagc gtttttgctg ataaaggcat
     2101 tacaattgaa tccatatata tgtgcccgca tgcaaaagaa gataactgtc attgcagaaa
     2161 accaaagacc tattttgttg atcaagctgt agcgtactat aatctggatg tacaacattc
     2221 ttttgttatt ggtgatcatc catctgatat actgcttgca aaaaatgctg ggatgcaggg
     2281 tatatatgtg ttgacagggc atggcaaaaa gcataaaaaa gaaattgata attgtgtgat
     2341 agttaaaaag aatttacagt atgcagtaga ttatatacta aaaagagtac aattatgaaa
     2401 aagccctttt ttgaaaaacc agcccgtatc tttgcacaca ggggatattc aaaagaatat
     2461 ccagaaaaca ccgcactggc gtttgaaaaa gcaattgcgt taggagcaga tgtcattgaa
     2521 acagatgcgc accttacccg ggatggacat gtggtgatag ttcatgatga ggaactatcg
     2581 cacataactg atggtaaggg aaatgttggc cagttaacat tacacgagat aaaacaatta
     2641 gatgcggcat atcatttcag cttggatgga gaaacttacc cataccgtgg caaaggtttt
     2701 actattatga cactggaaga gatgctggat gcttttcctg aacagcgctt taatgttgac
     2761 ctgaaagata ataccatagt gctggcagaa aaattttgtg atatagttct tagccataaa
     2821 tctgaacaca gggttctggg tgcatcgcag catggtaaaa atttacaata tatacgtaaa
     2881 agaatcccaa ccatggcaac atcatttggg ctgtggggtg tgttggggtt ttatttttta
     2941 ttcaggtcag gacttttggg gctttttaaa cattttcctg gtgatgcact gcaaataccg
     3001 gaatacattg ggccttcaca tctggcaaat ggggcactga tacgtatggc acatgagcgt
     3061 ggaatacagg ttgatgtgtg gacggtaaat caagaacgag atatgcggag actgcttgat
     3121 gtcaacgttg acggtatagt aaccgacgat gtaccactgc ttcaaaaagt acttcaggat
     3181 tatccattga agtagaagta tattttaatt ttattcataa cctgttgtgt
//
LOCUS       DAOSZP010000221         3220 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_23658_length_3220_cov_2.743444, whole genome shotgun sequence.
ACCESSION   DAOSZP010000221 DAOSZP010000000
VERSION     DAOSZP010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3220
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23658_length_3220_cov_2.743444"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            186..638
                     /gene="rplQ"
                     /locus_tag="PK348_05775"
     CDS             186..638
                     /gene="rplQ"
                     /locus_tag="PK348_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HXK65756.1"
                     /translation="MKHRNSVRTLGRSHEHRRAMFANMVTSLFQHERIITTKQKGKEL
                     KRIAERLITRAKENLSLTENDSARKLHNKREVMRLIKNEDVVKKLFDDIAPRFVTRPG
                     GYTRMFLLDRREGDAAQMAIVELVVKPETKKEDKKEDKKDKKGKKTTA"
     gene            complement(661..1512)
                     /locus_tag="PK348_05780"
     CDS             complement(661..1512)
                     /locus_tag="PK348_05780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65757.1"
                     /translation="MHKHLIILCSTIIVLIITAPSFAVSTAVKAQNDYFLTLSLLRQI
                     RIMVENFGDETTKNKYQEIQTLFQNASEEYYGQNFDSSHQKYYKVKQELVTLTDALAT
                     LYINRAQQILDSTSKQSFDIIIKYDKNSTLKKYFQKPYNPVEEIKPYNPEEYHLFFDR
                     ETIERYLKNGYKKLQEAKNIYNNQDIIYLKSKKNIKHDELEFIIKQYLAVIDFCRLAK
                     QYGIEIHKMVKVHQLDTIQRKYGLESNKLDPIYDDRIPEEFKVDANDNISLVHSIEKA
                     RLEKKVK"
     gene            1736..2611
                     /locus_tag="PK348_05785"
     CDS             1736..2611
                     /locus_tag="PK348_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015677547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2225 domain-containing protein"
                     /protein_id="HXK65758.1"
                     /translation="MENEDTKKKISFRQKNPTICPVCGYEFYREEMLTGGGRLIAGKL
                     TDELRRTYEKNEKWGVIYPLAYVVTVCPKCLYAAYPKDFPTLQADEMQKINATTNARK
                     QSIDKFFGGLDFNQDRGLYHGAASYLLAMDCYSFRNKNVAPTFKMAVSAIRAAWLFGD
                     LAKLEPDKPYKKISDFFYKKAYDYYFKVVDIIQTGAEPADAAGNMGPDIDKNWGYDGI
                     LYMCAILTLKVGAKEPDIHKRIENFERTKRYLSKLFGSGKASKSKPGPLLDMTRDLYD
                     KMNEMLDQWKVGEQE"
     gene            2608..>3220
                     /gene="truA"
                     /locus_tag="PK348_05790"
     CDS             2608..>3220
                     /gene="truA"
                     /locus_tag="PK348_05790"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HXK65759.1"
                     /translation="MRYALIVQYDGTAYSGFQIQDNANSIQHEIEKALKIFTRQNVRI
                     VAAGRTDAGVHALGQVVHFDLDTHVPADRLCISLNGILPADIAVKKAFVVDNLFHARY
                     SAIAREYLYVIYNHPYKSPFMRYRAMWVREPIDIEFCRKAATYCIGEKDFASFCKKTS
                     AEESTVRYIEYITVERMFDDVVVFTIKGNAFLHNMVRILVGTIL"
BASE COUNT         1009 a          500 c          728 g          983 t
ORIGIN      
        1 tcggtgcgtg catataatac attgaagagc ttagaattga atacacttga agatgttgta
       61 cgtaaaactg aagatgaact taaaaaatca aaacatttca gtgatcaggt actagctgaa
      121 attaaaaaga aattagaaga gcatcatttg agcttaggaa taaaggaata agaggtagtg
      181 acattatgaa acaccgtaat agtgttagaa cattagggcg ctcccatgag catagaagag
      241 caatgtttgc caatatggtg acatcgctgt ttcagcatga aagaataatt accacaaagc
      301 agaagggcaa agagttaaaa cggattgctg agcgattgat aacaagggcc aaggaaaact
      361 taagccttac cgaaaatgat agtgcacgta agctccataa caagcgtgaa gtaatgcgcc
      421 ttattaaaaa tgaagatgtt gttaagaagc tgtttgatga tattgcacca cgattcgtca
      481 cacgcccagg tggttataca cgtatgttcc ttcttgacag gcgtgaaggt gatgccgcac
      541 agatggcaat tgtagaactt gtggtgaaac ctgaaacaaa gaaagaagat aaaaaagaag
      601 ataaaaaaga taaaaagggc aaaaaaacca cagcataagc tgtggttttt tatttgatgt
      661 ctattttact tttttctcta atcgggcttt ctcaattgaa tgtactaaac tgatattgtc
      721 attagcatct actttaaatt cttcgggtat gcgatcgtca taaatgggat ccagcttatt
      781 gctttcaagg ccatatttac gctgtatggt gtccaattga tgcactttta ccattttatg
      841 aatttcaatt ccatattgtt ttgcaagacg acaaaaatcg ataactgcaa gatattgttt
      901 tatgataaat tcaagctcat cgtgcttgat attttttttg ctttttaaat agattatatc
      961 ctgattatta tagatatttt ttgcttcctg aagctttttg tagccattct ttaagtatcg
     1021 ttcaattgtt tccctgtcaa aaaaaaggtg gtattcttca gggttatatg gtttaatttc
     1081 ttcaacaggg ttatagggtt tttgaaaata tttttttaag gtactgtttt tgtcatactt
     1141 tatgataatg tcaaaggatt gctttgaggt tgagtccaga atctgctgag ccctgttaat
     1201 atacagggtg gcaagtgcat cggtgagagt aaccagttcc tgttttacct tataatactt
     1261 ctggtgtgag ctgtcaaaat tttgcccgta atattcttca gaagcatttt gaaaaagcgt
     1321 ttgtatttcc tggtatttat tttttgtagt ctcatcacca aagttttcaa ccatgatgcg
     1381 aatctgccgc agcagtgaaa gagtaaggaa atagtcattc tgtgctttca cggcagtaga
     1441 aacagcaaat gatggagccg tgatgataag cactataatt gtggaacaca gtataattag
     1501 atgcttatgc atatcattaa ccttcatatg atagtctttt ttattgtatc ggtaaaatat
     1561 ggaggctgct taacagtata tttttatagt agtagtaaaa ggaatgattt atcagttact
     1621 atcgccaaaa atttttaagc gatagttact gataaagtaa atatttgatt gcaaagatac
     1681 aggtgatact gttacatgaa aacaataact gtattttagt tgtggggatg aacgtatgga
     1741 aaatgaggat accaaaaaga agatatcatt caggcagaaa aatcccacca tatgtccggt
     1801 atgtgggtat gaattttatc gcgaggagat gctgacgggt ggtggcaggc ttatagcggg
     1861 caaattaacc gatgaattgc gcagaaccta tgaaaaaaat gaaaaatggg gagtgatata
     1921 tccgcttgca tacgtggtaa cggtttgccc taagtgtctg tatgcagcgt atccaaagga
     1981 ttttccaaca ctacaggctg atgaaatgca gaagataaac gcaactacca atgcacgaaa
     2041 acagtccatt gataagtttt ttggcgggct tgattttaat caggataggg gattgtatca
     2101 tggggctgca tcgtatttgc tggcaatgga ttgctattcg ttcaggaata aaaatgtggc
     2161 gccgacgttt aaaatggctg tcagtgctat acgggcagca tggctttttg gggatctggc
     2221 taagttagag ccggataagc cgtataaaaa gatttcggac tttttttaca aaaaggcgta
     2281 tgattattat tttaaggtgg ttgacatcat tcaaactggt gctgaacctg ctgatgcagc
     2341 aggcaacatg gggccggata ttgataaaaa ttggggttat gatggcatac tgtatatgtg
     2401 tgcaatactt acgttgaagg tgggtgcaaa agaacctgat atacataagc ggattgaaaa
     2461 ttttgagcgc acaaagcgct atttaagcaa actgtttggc agcggtaagg cgtcaaaaag
     2521 taagccgggc ccgcttcttg acatgacccg tgatttatat gacaagatga acgagatgct
     2581 tgaccaatgg aaagtcgggg aacaagagtg aggtatgcgc ttattgtaca gtatgatggt
     2641 actgcctaca gtggttttca aatacaggac aatgccaatt ccattcaaca tgaaatagaa
     2701 aaagcattga aaatttttac acgccagaat gtacggattg ttgcagcagg aaggactgat
     2761 gctggcgttc atgcattggg gcaggttgta cactttgatc ttgatactca cgtgcctgct
     2821 gacaggttat gcataagcct taatgggata ttgccggctg atattgcagt aaagaaggcg
     2881 tttgtagttg ataacctttt ccatgcacgt tacagtgcca ttgcgcgaga atatctgtat
     2941 gtcatttata atcatccgta caaaagcccg tttatgcgct atcgtgcaat gtgggtgcgt
     3001 gaacctattg atattgagtt ttgccgtaag gctgcaacat actgtatagg tgaaaaggat
     3061 tttgcttcat tctgtaaaaa aacatcagct gaagaaagca ctgtgcggta tatagaatat
     3121 attactgtag aacgtatgtt tgatgatgta gtggtgttta ctatcaaggg caatgccttt
     3181 ttgcataata tggtacggat actggtaggt acaatattgc
//
LOCUS       DAOSZP010000222         3217 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_23676_length_3217_cov_3.349462, whole genome shotgun sequence.
ACCESSION   DAOSZP010000222 DAOSZP010000000
VERSION     DAOSZP010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3217
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23676_length_3217_cov_3.349462"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..860
                     /locus_tag="PK348_05795"
     CDS             <1..860
                     /locus_tag="PK348_05795"
                     /inference="COORDINATES: protein motif:HMM:NF012506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HXK65760.1"
                     /translation="TDIKDVSRQCINIIAKAPVIIAYNYNVMRFRKGGDLFITKPNPA
                     WSTAENFLYLLRGERPDEYEAKLFDLALMLHAEHGGGNNSTFAVRTVTSSGANTYMAL
                     CAGVASLSGHLHGGANEAVMKMMKDIKQHVKDWNDDEEVEHYLKLMLEKKAYDRSGKI
                     YGIGHAVYTKSDPRAIIFRQKAEEFAKMKKNLDEFNLFAKVADIGSRLVQKYKGTVAS
                     PNVDFYSGLIYKMMGIPMELFTPIFAMARFSGWSAHRIEQIIHDKIIRPAYASSLDEF
                     KEYIPLEKR"
     gene            1000..2487
                     /locus_tag="PK348_05800"
     CDS             1000..2487
                     /locus_tag="PK348_05800"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HXK65761.1"
                     /translation="MYDVIIIGAGNAGLSAAATLARKGVKTLLIEKHNIPGGCATSFC
                     RGRFEFEVSLHQLSGLGTPERPGPLRSVLDSIGVLNKLEFVEMEKLYRVTLPGVIDVT
                     LPTNKEELIKVLQGKFPKEKDAIQKFFDFVWQFFTEVIGAFYLNDPDINPKKYPLYYK
                     YALKDTQSVLDEYFTDPILKAVISPYWTYMGLPPSKLCFSDMAALLFSYVEFKPYHIK
                     GGSQMLSNAMLEVVYENGGEVLFNTEVEKLLVKNNKVYGVRTSSGQEFESTYVLSNAS
                     AISTFVDMMDDAANHKVLEQYKGSSVGTSFFTIYCGLDCEYHEVGINEETNFISNTTD
                     MEFDYELSKTLETDKSSFILTCYNVFDNEMVPKGTSQVVLVTMKYAEPWLKVPPHQYN
                     DEKYRCAEAMLKNAEKVFPGLISHIEEMEIATPITHMRYLGHPGGGAYGFDQYVKDST
                     LFVSPKPPIDGLYCAGSWYGSAGYQPTLTSGHSAARAILKEMRNK"
     gene            2498..>3217
                     /locus_tag="PK348_05805"
     CDS             2498..>3217
                     /locus_tag="PK348_05805"
                     /inference="COORDINATES: protein motif:HMM:NF013161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HXK65762.1"
                     /translation="MDSQILKQLEGYDRVITDIDVLKRYGYDYRLERGLVKKYVERLH
                     PQIIDVKVIDIIEETKSTKTLRLASKQKYLPPFQAGQYISVIVEINGIRTSRPYSISS
                     SPDEIAYYDITVRRVEGGLVSNHLLDSLTIGDSLQISGPAGNFYHNPIIHGTKLVCIA
                     GGSGVTPFKSMIQHAVNTGTEREIWLIYGSKNADELIFDDYFAKVASKYKFFHYIPVL
                     EEGTGTYKGYITAKIIKEVVGD"
BASE COUNT         1067 a          508 c          693 g          949 t
ORIGIN      
        1 caactgatat taaagatgtt agcaggcagt gtatcaatat tattgcaaaa gcacctgtga
       61 tcattgcata taattacaat gtcatgagat tcagaaaagg tggcgattta tttattacaa
      121 aaccaaaccc tgcatggagt actgctgaaa actttttata tttgttacgt ggtgaaagac
      181 ctgatgaata tgaagctaaa ctttttgatt tagctcttat gttgcatgca gagcatggtg
      241 gtggaaataa ttccaccttt gcggtaagga ctgttacatc cagtggtgct aatacgtata
      301 tggctttatg tgctggtgtg gcctcattaa gtggtcattt gcacggtgga gcaaacgaag
      361 ctgtgatgaa aatgatgaaa gatataaagc agcatgttaa agattggaac gatgatgaag
      421 aagtggaaca ttatcttaag cttatgctgg aaaagaaagc ttatgacaga agtggaaaaa
      481 tatatggtat agggcatgca gtctatacaa aatccgatcc acgtgctatt attttcagac
      541 aaaaggctga agaatttgca aaaatgaaaa agaatttaga tgaatttaac ctttttgcaa
      601 aagttgcaga tattggttcg cgtttagtac agaaatataa aggaacagtt gcttcaccaa
      661 atgttgattt ttattcagga ttgatttata agatgatggg tattcctatg gaattgttta
      721 cacctatttt tgccatggca cggtttagcg gttggtcggc tcacaggatt gaacagatta
      781 tacacgataa gatcatacgg cctgcctatg cctcttctct ggatgaattt aaggaatata
      841 taccacttga aaaacgataa acatataata attgatcatt attatagaaa ttcaataaga
      901 gggggactat gaacccctct tttatttttt atttaaaaat agttgactaa aatagaatgt
      961 attttaacat aacaatgtta tgttatagga ggttctgtaa tgtatgatgt aataattatt
     1021 ggtgcaggaa atgcaggttt atctgcagct gccactcttg cccgaaaagg agttaaaacc
     1081 cttttaatag aaaagcataa tattccgggt gggtgtgcaa caagcttttg cagaggaaga
     1141 tttgagtttg aagtctcgct tcaccagtta agcgggttgg gtactcctga acgaccagga
     1201 cccttaagaa gtgtactgga tagcattggt gtgctgaata aactggaatt tgttgaaatg
     1261 gaaaaacttt accgggtaac tttgcctggt gtaatagatg ttacgctgcc gacaaataaa
     1321 gaggaactga ttaaagtgtt gcagggcaag ttcccaaaag aaaaagatgc aatacagaag
     1381 ttttttgatt ttgtatggca gttttttact gaagttatag gagcttttta cttaaatgat
     1441 ccagatatta atcctaagaa atatccctta tattataaat atgctttaaa agatactcaa
     1501 tcagtacttg acgaatattt tactgatccg atactgaaag cggtcataag cccatactgg
     1561 acatatatgg gactaccacc ttcaaagctt tgtttcagtg atatggcagc attgctgttt
     1621 tcttatgtag agtttaagcc atatcatata aaaggcgggt cgcagatgct gtcaaatgca
     1681 atgcttgaag tggtatatga aaatggagga gaggttttat ttaacactga agttgaaaaa
     1741 ctactggtaa aaaataataa agtttatggc gttcgcactt catcagggca ggaatttgaa
     1801 tcaacgtatg tgctgtcaaa tgcttcagca ataagcacct ttgtagacat gatggatgat
     1861 gcggcaaatc acaaagttct ggagcaatat aaggggagca gcgtaggaac atcttttttt
     1921 accatatatt gcggactgga ttgtgaatat cacgaagtcg gcattaatga agaaacaaac
     1981 tttatcagca ataccacaga tatggaattt gattatgaac tatcgaaaac acttgaaact
     2041 gataaaagtt cattcatttt aacctgctat aatgtttttg ataatgaaat ggtgccaaaa
     2101 ggcacatcgc aagttgtact ggttaccatg aagtatgccg aaccatggct taaagtgccc
     2161 cctcatcagt acaatgatga aaaataccgt tgtgctgaag ccatgttaaa aaatgcagaa
     2221 aaagtttttc caggccttat tagccatatt gaggaaatgg aaattgctac accaattaca
     2281 catatgcgtt atttgggcca tccaggtggt ggagcatacg gttttgacca gtatgtaaaa
     2341 gactctacgt tgtttgtttc gccaaaaccg cctattgatg ggctgtattg tgcaggcagc
     2401 tggtatggtt cagctggtta tcagccaaca cttacttcag ggcattcagc agcaagagct
     2461 atcctgaaag aaatgcgaaa caaataggag ggaacacatg gacagccaga tattaaaaca
     2521 acttgaaggg tatgacagag ttataactga tatagatgtc ctcaaacgtt atgggtatga
     2581 ttacagactg gaacggggac tggttaaaaa atatgttgaa cggttgcatc cacagataat
     2641 tgatgtaaaa gtaattgata ttatagaaga aacaaaatcc accaaaacat tacggctggc
     2701 atcaaagcaa aagtatctgc caccatttca ggctggacaa tatatatcag tgattgttga
     2761 aataaacgga atacgcacat cacgtccgta cagtatatcg tcttcccctg atgaaattgc
     2821 atattatgat ataacggtac gtcgtgttga aggcggtttg gtttctaatc atctacttga
     2881 tagccttaca attggcgata gtttacagat atcaggtcct gcaggaaatt tttatcacaa
     2941 tccaatcatt catggaacaa agcttgtatg tattgcaggt ggtagcggcg tgacaccatt
     3001 taagtccatg attcagcatg cggtcaatac gggaactgaa agggaaatat ggcttatcta
     3061 tggaagtaaa aatgcagatg aactgatttt tgatgattac tttgcaaagg tagcgtccaa
     3121 atataaattt tttcactata tccctgtact tgaagaaggc acgggcacct ataaagggta
     3181 tattacagca aaaatcataa aagaagtggt gggcgat
//
LOCUS       DAOSZP010000223         3213 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_23715_length_3213_cov_3.385687, whole genome shotgun sequence.
ACCESSION   DAOSZP010000223 DAOSZP010000000
VERSION     DAOSZP010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3213
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23715_length_3213_cov_3.385687"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            28..1614
                     /locus_tag="PK348_05810"
     CDS             28..1614
                     /locus_tag="PK348_05810"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HXK65763.1"
                     /translation="MKTNYTARQVLEIKSSGTYIIIFIIIAVIAHIFQILGKVDILEI
                     LRLSLISTICVILAYVIYKRKKLLKATGVFEWILGFISVNIPLAAKFAYAQKYDWTFA
                     LESYNSSVLMVILLVMLQLYFNRRLMAFYTGYTVVTWTAFLLVAIAHGAVIHWDATLD
                     GKPVHGFIIFREIFFIMITAVFGILASRNIKVVQQYDDRSTRQLSVIQQQSDMQRQLN
                     ETIKEKVADLLEQVKQQDILTIKFNEKMQNQASTFEEISATLEELLGSAESIARISEE
                     QVGGNEMMETIVSEFKVIKNETRQNLDKTIQEVENVVARTGNANERIKEVEATINQIR
                     EQSQKIGDTINVIVDIADKINLLSLNASIEAARAGEYGRGFAVVADEIGKLAFQTSES
                     IKDIEKVLIQSTKTTETGVEVIRTTAEMLKDLIARMAESSSKIKILQDSVTVEEKYIK
                     NIIAQMEKNVELAKNIGAGTEEQKAAIETSAKAIEHMNEVVAQMVDEIQQLAHTSRSI
                     LDNATALLEKSRGDSSLDTI"
     gene            1643..2296
                     /locus_tag="PK348_05815"
     CDS             1643..2296
                     /locus_tag="PK348_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015194725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HXK65764.1"
                     /translation="MQHTNTTYCIFCDFDGTITTVETFVGMCKTFAPGLFDEIGKKMW
                     NKELTIRQGVRQLLESLPSSLYSDMLAYIDDKEIRPGFEEFLEYCNNRSIPVIIISGG
                     LYDSVVRKLGNLKNKVTAIYAAKIDASGKYLKVYSDYEHEGELVAKANIMSNYRHTCK
                     IAIGDGPTDIEMAKYADIVFARGLLKKFLPEDKEYYEWDDFFDVMDTLSIIIEQEKF"
     gene            2384..2944
                     /locus_tag="PK348_05820"
     CDS             2384..2944
                     /locus_tag="PK348_05820"
                     /inference="COORDINATES: protein motif:HMM:NF027363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chalcone isomerase family protein"
                     /protein_id="HXK65765.1"
                     /translation="MRKSVVVVIALLVAVCMMSDVFSLSLGGKDLVKKGTGKRTKGIL
                     GTVYYATLWVPESLKTADAKAIVEADEPMAVVLVIDSVLLNKERFLEATKEGFAQAAS
                     SGYATAKQDAFLKLFDAVEFKKGDVIQLGYDPKAGITAVFTASATKKTTTLGSVAGLD
                     LKKALFAIWLGPKPVQDSLKKAMLGM"
     gene            complement(3011..>3213)
                     /locus_tag="PK348_05825"
     CDS             complement(3011..>3213)
                     /locus_tag="PK348_05825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65766.1"
                     /translation="FANNPVRAGKVKSPFESYFGGSRAYFDRNYRPTVKITLHQYFLE
                     LGNTLEECIERLCAYCRGELLL"
BASE COUNT         1068 a          534 c          717 g          894 t
ORIGIN      
        1 ctttgaataa cattttgtga ggttcctatg aaaacaaact atacagcccg tcaggttctg
       61 gaaataaaat caagtggaac atatattatt atatttatta taatcgctgt cattgcccat
      121 atattccaga tattggggaa agttgatatt ctggaaattt tacgattatc cttaatcagc
      181 actatctgtg ttatactggc atatgttatt tataaaagaa aaaaattact gaaagcaact
      241 ggcgtgtttg aatggattct gggatttatc agtgtcaata tacctttagc agccaagttt
      301 gcctatgcgc aaaagtatga ctggacattt gcacttgagt cgtataacag ttcagttctt
      361 atggttatat tactggttat gttgcaactg tacttcaacc gacggcttat ggccttttac
      421 actggctata ctgtagtaac atggactgca tttttattgg tggcgatagc tcatggagcg
      481 gttatacact gggatgcaac acttgatggc aagccagtcc acggctttat tatatttcgc
      541 gagattttct ttatcatgat tacagcggtg tttggaatat tagcttcacg caatataaaa
      601 gtagtacagc aatatgatga tcgctccacc agacaacttt ctgttattca gcagcaatcg
      661 gatatgcagc gacagctcaa cgagactata aaggaaaagg tagcagattt actggaacag
      721 gtaaaacagc aggatatact cactataaaa tttaacgaaa agatgcagaa tcaggcatca
      781 acatttgaag agatatcagc aacactggaa gaactgctgg gctcagcgga aagcatagca
      841 cgcatttccg aagagcaggt gggcggtaat gaaatgatgg aaaccattgt cagcgagttt
      901 aaagttataa aaaacgaaac gcggcaaaat ttagataaaa caattcaaga agttgaaaat
      961 gttgttgcgc gtacaggcaa tgctaacgaa agaatcaaag aggttgaagc aaccatcaat
     1021 cagatacggg agcaaagtca gaaaattggt gacactataa acgtaatagt tgatatagca
     1081 gataagataa atttactgtc actcaacgcc agcattgagg ctgcgcgtgc aggtgagtat
     1141 ggcaggggat ttgcagtggt tgcggatgaa attggcaagc tggcatttca aacatcggag
     1201 agcatcaaag atattgaaaa agtgctgata cagagcacaa aaaccactga aactggtgtt
     1261 gaggttatca ggacaactgc tgaaatgtta aaggatctga ttgcccgtat ggccgaaagt
     1321 tcctcaaaga ttaaaatatt gcaggatagt gttaccgttg aagagaagta cataaaaaat
     1381 atcattgccc agatggaaaa gaacgttgaa ctggcaaaaa acattggtgc tggcactgaa
     1441 gaacaaaaag ctgcaattga aacaagcgca aaggccattg aacatatgaa tgaagttgtt
     1501 gctcaaatgg ttgatgagat acagcagctg gcacacactt cccgaagtat tttggataat
     1561 gcaacagcgc ttttagaaaa atcgcggggc gatagttcat tggatactat ataatataat
     1621 gtaatgtaag aaccatcaaa taatgcaaca taccaatact acatattgca tattctgtga
     1681 ctttgatggc acgataacca ccgttgagac atttgttggt atgtgtaaaa cctttgctcc
     1741 aggtttattt gatgaaattg gcaaaaagat gtggaataag gagcttacca tcaggcaggg
     1801 tgtgcgacag ctacttgaat cgttgccctc ttcattatat tcagacatgc ttgcttatat
     1861 tgatgataaa gaaatacggc caggatttga ggagtttctg gaatattgca ataacagatc
     1921 aatccctgtt attatcatat caggcggatt gtacgatagt gtggtccgaa aattaggcaa
     1981 tttgaaaaat aaggttacgg caatctatgc agcaaaaatt gatgctagcg gtaaatatct
     2041 caaagtgtat tctgattatg agcatgaagg tgagttggtg gcaaaggcta acattatgag
     2101 taactatcgc cacacatgta aaatagccat aggcgatgga ccaaccgata ttgagatggc
     2161 aaagtatgct gatattgtgt ttgccagggg tcttttgaaa aagtttttgc ctgaagataa
     2221 agaatactat gaatgggatg atttttttga tgttatggat acactgtcaa ttatcattga
     2281 acaggaaaaa ttttaattga ctattgtata aatacaatgt atgtactcta tgaccatcaa
     2341 ctatatgtaa taaaaaaatt accataacgc aaggaggtta aacatgcgta aaagtgtagt
     2401 agttgttata gcattactgg ttgctgtttg catgatgtct gatgtttttt cgctgagtct
     2461 tggaggaaaa gatctggtga agaaaggtac tggcaaaaga acaaagggga ttttaggtac
     2521 agtgtattat gcaacattgt gggtacctga aagccttaaa acagccgatg caaaggcaat
     2581 agtagaagct gatgagccaa tggctgttgt gctggtgatt gactctgtgc ttcttaataa
     2641 agaaagattc cttgaagcta caaaagaggg ttttgcacag gctgcatcaa gtggatatgc
     2701 aactgccaaa caggacgctt tcttaaaact ttttgatgct gtcgaattta aaaaaggtga
     2761 tgtaattcag cttggatatg accccaaagc aggaattaca gcagtattta ctgcgtctgc
     2821 aacaaagaaa acaaccacat tgggttctgt tgcaggactt gaccttaaga aggcattatt
     2881 tgcaatatgg cttggaccaa agcctgttca ggatagcctg aagaaggcaa tgttgggtat
     2941 gtaattattt taatgcgtgt atactctgct atgcaagtgg ggtatacacg tattatcaaa
     3001 gatcaaaggg tcagagcaaa agctcgccac ggcaataagc acacagacgt tcaatacact
     3061 cttccaatgt gttacccagc tctaaaaaat attgatgcag ggtaatcttt acagtagggc
     3121 gatagttcct gtcaaagtaa gcccttgacc caccaaagta gctttcaaaa ggacttttca
     3181 ccttgcctgc acgcaccgga ttgttggcaa aat
//
LOCUS       DAOSZP010000224         3203 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_23811_length_3203_cov_3.455845, whole genome shotgun sequence.
ACCESSION   DAOSZP010000224 DAOSZP010000000
VERSION     DAOSZP010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3203
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23811_length_3203_cov_3.455845"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..507
                     /locus_tag="PK348_05830"
     CDS             <1..507
                     /locus_tag="PK348_05830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HXK65767.1"
                     /translation="CLLQLGQYYEYNKMYNEAWSTYNFLMSQFPKSPEAVKANTYAQN
                     VRKYNPKTVAFMPDEKIIAQSEAIDIKPDVEESDTQQNLKLYYTISIGPFYNLQKAKE
                     LQKLIVNYVVPVHIVRKEKSFDIYAGHFPSLDEGMSIKIQLAEELGINGFIIEIFEQD
                     GKQYIYGQ"
     gene            497..3058
                     /gene="mutS"
                     /locus_tag="PK348_05835"
     CDS             497..3058
                     /gene="mutS"
                     /locus_tag="PK348_05835"
                     /inference="COORDINATES: protein motif:HMM:TIGR01070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HXK65768.1"
                     /translation="MGNDKELTPMMRQYLEIKQHYKDEILFFRLGDFYEMFFDDAKIA
                     SKVLDIALTSRQNDIPMCGVPYHAADSYIARLIKAGYRVAICEQMEPPSSSTIVKREV
                     IRVITPGTVVESNLIASDENNYLGSVVIHNNTVCTGFVDVSTGDFFLSSADYSREFIA
                     TEISRFSPKEMLLYGSQQELTFLHDLLKQQDIALASINEWYYDVEYLTNIICNIYGLS
                     NIKGLGLTHTAILTVGSLLHYLKDTHKSALVHLKYPKNVSRSEFMVLDHQTIHNLEIL
                     KSQDGSKLHSLFGVLNHTQTAMGKRTLERALLYPLLDASALESRYDIIAYLIDNVQLL
                     KEIQEKLSRIHDLERIVSRFALKKYYNRDFIALQQSIEAASDVYAVLQQHPDNLFNML
                     GTISDSLQELSLKIKNTIVDNPPLSSEHGRVIKEGVNAELDRLHTLKTDARQWLLNYQ
                     EEEKRRLDIPTLKVRYNKVIGYYIEVSKGQTGKVPQDYFRKQTLINAERYTTDTLQKF
                     ETDVLQAHDSIIKIETEILESIVETILANKLQIQELAYTIGILDMFCSLALAAYQNNY
                     IRPMLNNDGIIDIKAGRHPVVEKFYIKEPFVPNDIFLDTVENVIAIITGPNMSGKSTY
                     IRMAAVLQLMAQIGSFIPAQLANLCIVDRIFTRIGASDNIARGESTFLVEMNETAIIL
                     NNATDKSLIIMDEIGRGTSTYDGMSIAWAVVEYIANYIKAKTLFATHYHELTKINKKG
                     IVNYTVMVKESISGVEFLHKVVPGAADKSYGIHVAKLAGIPKPVTVKANKILEKLEKS
                     SRIDSLIKAPDNEEQLEIFQATHHRLAQALKDITVENLTPLEALNEIARLKRLIE"
     gene            complement(3060..>3203)
                     /locus_tag="PK348_05840"
     CDS             complement(3060..>3203)
                     /locus_tag="PK348_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HXK65769.1"
                     /translation="KLMEPHPIALQLRYLQTLREVATEKNSTTLFPIPIDLVEPFLAG
                     RKK"
BASE COUNT         1077 a          540 c          615 g          971 t
ORIGIN      
        1 tgtttactgc aactgggaca atactacgaa tataataaaa tgtataatga agcatggtca
       61 acgtataatt ttttgatgag ccaattcccc aaatcaccag aagcagtgaa ggcaaatacg
      121 tatgctcaaa atgtcaggaa gtataatcct aaaactgttg catttatgcc tgatgaaaaa
      181 attattgcac agagtgaggc tattgatatt aaacctgatg tagaagagag tgacacacag
      241 caaaatctaa aactttacta taccatttct attggacctt tttataattt acaaaaagca
      301 aaagaattgc aaaaattaat tgtcaattat gtggtgccag tacatatcgt aagaaaagaa
      361 aaatcttttg atatttatgc aggccatttc ccttcacttg atgaaggtat gagtataaaa
      421 atacagttgg ctgaggagtt aggcattaac gggtttataa ttgaaatttt tgaacaggat
      481 gggaagcagt atatctatgg gcaatgataa agaactaact ccaatgatgc gacagtatct
      541 tgaaatcaag caacattaca aagatgagat actgtttttc aggcttggcg atttttacga
      601 aatgtttttt gatgatgcaa agattgcttc aaaagtttta gatattgcac tgacttctag
      661 gcaaaatgac attcccatgt gtggagtgcc ataccatgct gctgacagct acatagcaag
      721 gcttatcaaa gcaggatacc gtgtggcaat atgtgaacag atggaaccac catcttcaag
      781 cactattgtt aagcgggaag ttatccgcgt cattacacca ggtacggttg ttgaaagcaa
      841 tcttattgca tcagatgaaa acaattacct gggttcggtg gttattcata acaatacggt
      901 ttgtactgga tttgttgatg tatctacagg cgattttttt ctttcatctg cagattatag
      961 tcgtgaattc attgcaactg aaatttcccg cttttcacca aaagagatgt tattgtacgg
     1021 ttcacagcag gaacttacgt ttttgcatga ccttttaaaa cagcaggata ttgctctggc
     1081 atctattaat gaatggtatt atgatgttga atatcttact aatataattt gtaatatcta
     1141 tggattaagc aatattaagg gattgggatt aacacataca gcaattctta cagttggttc
     1201 actgcttcac tatttaaaag atacgcataa aagtgcactg gttcacctta aatatccaaa
     1261 aaatgttagc cgcagtgaat ttatggtttt agaccaccaa actatacaca atcttgaaat
     1321 attgaaaagc caggatggct caaaacttca ctcacttttt ggtgtgctta atcacacaca
     1381 aactgcaatg ggaaagcgaa cattagaacg ggctcttttg tatccattgc ttgatgcatc
     1441 tgcacttgaa agtcgttatg atattatagc atatcttatt gacaatgtac aattgttaaa
     1501 ggaaattcag gaaaaattat cacgtattca tgatcttgaa cgtattgttt ctcgttttgc
     1561 attgaagaaa tattacaaca gggatttcat tgctctacaa caatccattg aagctgccag
     1621 tgatgtatat gcagtattgc agcaacatcc tgacaacctt tttaatatgc tgggtactat
     1681 cagcgattct ttacaggaac tatcgctcaa aattaaaaat actatagttg ataatccacc
     1741 actttcctca gaacatgggc gtgttataaa agaaggtgtt aatgctgaac ttgatagact
     1801 gcatacgtta aaaactgatg cacggcagtg gcttctcaat tatcaggaag aagaaaaaag
     1861 gcgtcttgat attcctacat taaaagtacg ctacaacaaa gttattggct attacattga
     1921 agtgagcaag ggtcagactg gaaaggtacc acaggattat ttcaggaaac aaacgttaat
     1981 taatgctgag cgatatacaa ctgatacact acagaagttt gaaacagatg tgctgcaggc
     2041 acacgattca attatcaaga ttgaaactga gatacttgag tcaattgttg aaacaatact
     2101 agctaacaag cttcagattc aggaattagc atatactatt ggcatactgg atatgttttg
     2161 ttcattggct cttgcagcat atcaaaataa ttatattagg ccaatgttga acaatgacgg
     2221 tattattgac attaaggctg gcagacaccc tgttgttgaa aaattttata ttaaagaacc
     2281 ttttgtcccc aatgacattt ttttagatac agttgaaaac gttattgcta ttatcacggg
     2341 ccctaatatg tcaggtaaat caacgtacat acgaatggct gctgtattgc agctaatggc
     2401 tcagattgga tcatttatac ccgcacaatt agctaatctg tgtattgttg atagaatatt
     2461 tacacgtatc ggtgccagtg acaatattgc acgcggtgaa tcaacatttc ttgttgaaat
     2521 gaacgaaaca gctattatct taaataatgc tactgataaa agcttgatca ttatggatga
     2581 gattggacgt gggacaagta cttacgatgg gatgtcaata gcctgggcag ttgttgaata
     2641 tattgccaat tacatcaagg caaaaacatt atttgctact cactatcatg aattaactaa
     2701 aataaataaa aaaggtattg ttaattatac agtgatggtg aaagaatcta ttagcggggt
     2761 tgaattttta cataaagttg ttcctggtgc ggcggacaaa tcgtatggga ttcatgtggc
     2821 aaaacttgct ggtattccaa aacccgtaac tgtaaaagca aacaaaatcc tggaaaaact
     2881 tgaaaaatct tcacggattg attcattaat taaagctcct gataatgaag aacaacttga
     2941 aatattccag gcaacgcatc acagactggc acaggctctt aaagatataa ccgttgaaaa
     3001 tttaacaccg cttgaagcgc tcaatgaaat tgcacgcctc aagcggctta ttgaatgatt
     3061 catttcttac ggcctgctaa aaatggttca accagatcaa ttggtattgg gaacagtgtg
     3121 gtagaatttt tctcggtagc aacttcacgc aacgtctgta aataacgaag ctgcaatgca
     3181 ataggatgcg gttccataag ttt
//
LOCUS       DAOSZP010000225         3178 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_24033_length_3178_cov_2.933718, whole genome shotgun sequence.
ACCESSION   DAOSZP010000225 DAOSZP010000000
VERSION     DAOSZP010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3178
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24033_length_3178_cov_2.933718"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..960)
                     /locus_tag="PK348_05845"
     CDS             complement(<1..960)
                     /locus_tag="PK348_05845"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HXK65770.1"
                     /translation="MKIFTISELIGTMKQFPLLQKVSPVEAVKSLTFFKDVPEAVLQE
                     IVDGIFIHQYAKDEIISRHGRYNEWLYVVLSGEISVFIITPDYTKLELYALGPEDFFG
                     EDIVIRNEPRESTAIAFTDSILLAIGKQELTKIIASSAATYEKLNNAFLQRKMRNNLR
                     SIPIFTHLREEVFNEILDVVRLVHVKKGDVIFKQGDVGDALFLIRKGDVSVYRAMNKN
                     EELISLLAEGNFFGEMALVLGEPRNATVIANDDCELLKIDKNDFDSIIARHVDVYNTI
                     QAVALERVTGHELFDSNEAFISKKLIELNRAINKHIDVIAQCTF"
     gene            complement(1047..2708)
                     /locus_tag="PK348_05850"
     CDS             complement(1047..2708)
                     /locus_tag="PK348_05850"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65771.1"
                     /translation="MKKVNIKKYYAITGIIILSVLCIALTYDTTYQIMFTSNGLDLFN
                     VKKIKLQGNQSGFTIAKINYKESVNPLVTDLLITFDKDFPAMVKDDSGHYAIRYAQYT
                     YTKEDIENEGCAYFYHKDHRIEIQTAKNNWLTNCTDLGSFTIDAKLKLTKYSPLAVVL
                     SRIGYSGGKQGFEIVCVNDKLAVRFYKMFYKKDNVPVDVFCHRSPSLALNTWYHIAVS
                     FDRISGKLETMINGDIIETIFCTENGEPFVDVMVPRFICQDIPVAVVGKHFYGYLDKI
                     RIAQRYIVDLEKETEIAKTQFKAITYNQRDTINREGIITSPVYTFPDYGTMVTLFAWD
                     EKMPPSTYIWMEFRISDAFFKANDETPQWYRITNKQKNIYLHRVGNEYLRGKFYQWRA
                     HLIASSDGSNAPVIYNIGLQYALDTAPQPPIFVTVDSVGNNMVTLKWKKNVEYDILGY
                     NIYYGVKSKQYDGIIRTVNGKRISNELSDTNGYITVTLTNDVIEENRANDTKGFLTFP
                     VLKNNVLYFFAVTAYDSYKPDTMFNHESKHSKEVSARPDPGSDIK"
     gene            complement(2705..3142)
                     /gene="rpiB"
                     /locus_tag="PK348_05855"
     CDS             complement(2705..3142)
                     /gene="rpiB"
                     /locus_tag="PK348_05855"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012057395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HXK65772.1"
                     /translation="MKIAIGNDHAGVELKQYIMQHFKEHNFINVGTDVTQSCDYPDYI
                     KLVAELVQKGEVDYGIALCGTGIGASIVANKYKGIRAALCTNEFMATMARRHNDANIL
                     VLGARVVGMDLAIHITAAFLQGTFEGGRHQRRLDKIAQIEIME"
BASE COUNT          946 a          681 c          494 g         1057 t
ORIGIN      
        1 aaacgtacat tgtgcaataa catcaatatg cttatttatt gccctattta attctataag
       61 cttttttgat ataaatgctt cattgctgtc aaaaagttca tgccctgtca cccgctcaag
      121 tgcaacagcc tgaatagtat tatagacatc aacatgacgc gctataatgg aatcaaaatc
      181 atttttatca atcttcagca attcacagtc atcgttagct ataacagtag cattccgagg
      241 ttcacccagt accagggcca tctcaccaaa aaaattaccc tcagcaagta acgatatgag
      301 ttcctcattt ttattcattg ctctgtaaac actcacatcc ccttttctta ttaaaaacaa
      361 tgcatcccca acatcaccct gtttaaaaat cacatctcct ttttttacgt gtaccagcct
      421 gacaacatcc agtatttcat tgaaaacctc ttcgcgcaga tgtgtaaaaa ttggtatgga
      481 ccgcagattg ttacgcattt tccgctgcaa aaaggcatta ttgagttttt cataagttgc
      541 agccgaagat gcaataattt ttgttaattc ctgcttccca attgcaagta aaatactatc
      601 agtaaatgct atcgccgtac tttcccgtgg ctcatttcgg attacaatgt cctcaccaaa
      661 aaagtcttca ggccccaatg catataactc cagtttggta taatcaggcg taataataaa
      721 tactgatatt tcacccgaaa gtacaacata aagccactca ttgtatcttc catgcctgct
      781 gataatttca tcttttgcat actgatgaat gaatatgcca tcaactatct cctgtaaaac
      841 cgcttcaggg acatctttaa agaatgttaa agactttacc gcctcaacag gcgatacctt
      901 ttgcaaaaga gggaattgct tcattgtccc aataagttca ctgatagtaa atatcttcat
      961 ataattacct ttcattaatt tttgatcaat tttatttaat aatagagtat tataatgaca
     1021 attgcaagta taattgaagt ttcgttctac ttaatatctg atcctgggtc agggcgggca
     1081 cttacttctt ttgaatgttt tgattcatga ttgaacatgg tatcaggctt atatgaatca
     1141 taggcagtca ctgcaaaaaa atacagaaca ttgttcttta ataccggaaa cgttaaaaac
     1201 ccttttgtat cattggcacg gttttcttca atgacatcat ttgttagtgt tactgtaata
     1261 tatccatttg tgtccgacag ttcattagaa atgcgtttgc cattaacagt tcgtataatg
     1321 ccatcatatt gtttgctttt aacgccatag taaatgttat accccagtat atcgtactct
     1381 acgttcttct tccacttcag cgtgaccata ttgttaccaa ctgaatcaac agtgacaaaa
     1441 atgggtggtt gcggggccgt atctaatgca tactgcaatc caatattgta gatgaccggc
     1501 gcatttgatc catcggatga agctattaaa tgtgcccgcc actgatagaa tttgcctctg
     1561 agatattcat tgccaaccct gtgcagatat atattttttt gcttatttgt tattctatac
     1621 cattgtggtg tttcatcatt tgctttgaag aacgcatcac tgattctaaa ttccatccat
     1681 atatatgtgg atgggggcat cttttcatcc caggcaaaaa gcgttaccat tgtcccataa
     1741 tccgggaacg tatatacagg gcttgtgatg ataccctccc ggtttatagt atccctctga
     1801 ttataggtta tagctttaaa ttgtgttttt gctatttcag tttctttttc taaatcaaca
     1861 atatatcgct gggcaattct gatcttatct aagtacccat aaaaatgttt ccccaccaca
     1921 gcaaccggta tgtcctgaca tataaaccgt ggtaccataa catctacaaa tggctcacca
     1981 ttttcagtac aaaatattgt ttctataata tctccattta tcattgtttc taatttgcca
     2041 gatattctgt caaaactaac cgcaatatga taccaggtat tcaatgcaag cgacggtgac
     2101 ctgtgacaga aaacatccac cggcacatta tcctttttat aaaacatttt ataaaaccta
     2161 acggcaagct tatcattaac acacacaatc tcaaaaccct gtttaccgcc tgaataccca
     2221 attcgcgaaa gaactaccgc caggggactg tatttggtaa gcttcagttt tgcatcaatg
     2281 gtaaatgatc ctaaatcggt acagtttgtt aaccagttat tctttgcagt ttgtatttca
     2341 atacggtggt ctttgtgata aaaatatgca catccttcgt tttcaatatc ttcttttgta
     2401 tatgtatact gtgcgtatcg tattgcataa tggccagaat catccttgac catggcaggg
     2461 aaatctttat caaaggtaat caataaatca gtaaccagtg gattgacgga ttctttatag
     2521 tttatttttg cgatagtaaa tcctgattgg ttcccctgca atttaatttt ttttacatta
     2581 aacagatcca acccattaga agtaaacatg atctggtatg tggtatcata cgtcagcgca
     2641 atacacaaca ccgacagaat aataatacct gtaatagcat aatatttttt tatattgacc
     2701 tttttcattc cattatttct atttgtgcaa ttttatctaa cctgcgctga tggcggccac
     2761 cttcaaatgt gccttgtaaa aatgctgctg ttatgtgaat agctaaatcc attccaacaa
     2821 cccgcgcgcc cagcaccaga atatttgcat cattgtgcct tcgtgccatg gtagccataa
     2881 actcattggt acacagggca gcacgaatgc ctttatactt attggcaaca atggatgcac
     2941 ctattcctgt accacatagt gctatgccat aatccacttc ccctttttgc accagttcag
     3001 caactaattt aatataatca ggataatcgc atgattgtgt gacatcagtg ccaacattaa
     3061 taaaattatg ctctttaaaa tgctgcataa tatattgttt taactcaacg cctgcatgat
     3121 cattgccaat tgctattttc atatacaatt cacctgctaa aatcgtgcgt ttcccggc
//
LOCUS       DAOSZP010000226         3177 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_24041_length_3177_cov_3.790519, whole genome shotgun sequence.
ACCESSION   DAOSZP010000226 DAOSZP010000000
VERSION     DAOSZP010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3177
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24041_length_3177_cov_3.790519"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..614
                     /locus_tag="PK348_05860"
     CDS             <1..614
                     /locus_tag="PK348_05860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65773.1"
                     /translation="SPPSGFIIPDTITVTYVNNDDNDSSDKKELILWNNEIQKALLQQ
                     GIQIINENRTITLFDDTKQQNQHPSLIIDTLRIEKISETLSVSHKHSDITAIYPYHYY
                     FAEINASISVHNVIVWRGSVRLTSFDLFQQKKLLTRPEVYFTLTSNYSYSNKMKQWIS
                     TNPTISLSDNFSKYYDSSIDSELHKQQLIALAVTTLLSNIKGE"
     gene            614..1198
                     /locus_tag="PK348_05865"
     CDS             614..1198
                     /locus_tag="PK348_05865"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXK65774.1"
                     /translation="MRTFTSIIVLLFYIACSNTNHAKELYNKALTRYANKDFEGASTL
                     CLESIKKADLPDSYLLLAKIYFFTNNSYFEKTIEKYIRLTDSSQGYILYARWFIRNQK
                     QDQAIRYLEKALLHSPNDPVALYLLGTIQYANKQYDDAIITFHRAFANYFPLMQIHRQ
                     LQEIYSEIHLPERAAKHQAILEAIKQFDDSTNGE"
     gene            1201..1665
                     /locus_tag="PK348_05870"
     CDS             1201..1665
                     /locus_tag="PK348_05870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65775.1"
                     /translation="MKKLMIMVSALVFFTACKSLDIQTVQHAPFANDSIFIKDVRIHN
                     ELYSTIKDSLVSHIVFHLQKYNLKAASYFNPIYREQDFKYTATVDIFITKTGDIFNQV
                     ENASLFIFINDASMHIASIRIISQSCRLQESFDQALIAKAIAKEIDAMVVKK"
     gene            1662..3017
                     /locus_tag="PK348_05875"
     CDS             1662..3017
                     /locus_tag="PK348_05875"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HXK65776.1"
                     /translation="MKKFFCIFAVYIIGTCSAWSQETLTLHNACELALKNSVKLERLK
                     AKIQSSRMIVHERWRELLPNVSFQYSKSDTVAIREDDTRFQSLACTVQYDIFTNQQSL
                     IAYSIAQIESMLANEEYTIEKNNIILSTKQAYFALQKKRKSIEIYQLLLKSLQLQKNI
                     ITAQQQLGMATELELIQVDGKIAEAQYNILSAQNQYANSLQDFATTLGINYASIQLPE
                     QEYTELQNITMASKENLISLSMQNRNELKKSYYTLIKTQKEYQLAQYYYLPRIQLFAS
                     YGYSGTEFPMNKKMWNVGLSVTSLLFGNTASLSNSYGKKDNGNTTDIKSSASVAVYEN
                     PSFIRSIIDAEAAYTEAQKYHQQLIQIIRTDVSKAYDTVMESLKKVEIARQTVLLLEK
                     QSSIENEQARLGDITRYDVLKTLVELSQARLRLQETITDVFVSMAALENAVGLPIESI
                     F"
BASE COUNT         1136 a          568 c          517 g          956 t
ORIGIN      
        1 tgagcccacc atctgggttc attattccag atactatcac tgttacatat gtgaacaatg
       61 atgataatga ttcaagtgat aaaaaggaac tcattctatg gaataatgaa atacagaaag
      121 cattattaca gcagggaatt caaataatca atgaaaatag aacgattact ctgtttgatg
      181 atactaaaca gcagaaccaa catccatcgt taataatcga cacactccgt attgagaaaa
      241 tatctgaaac tctatcggta tcgcacaaac attcggatat tacagcaatc tatccctatc
      301 attactattt tgctgaaatt aatgcaagta tttctgttca taatgtgatt gtgtggcgtg
      361 gtagtgttcg tttaacatca tttgacttat ttcaacaaaa gaagctgtta accagacctg
      421 aagtttactt tacgctaaca agcaattatt cttattcaaa taagatgaaa caatggataa
      481 gcaccaatcc taccatatcg ttgtctgaca atttcagtaa atactatgat tccagtatag
      541 acagcgaact gcacaaacaa cagcttatag cattggctgt aacaacacta ctatcaaata
      601 tcaaaggtga ataatgagaa catttaccag tataatagta cttttgttct acattgcttg
      661 tagcaatacc aatcatgcaa aagaattgta taacaaagca cttacacgtt atgcaaacaa
      721 agattttgaa ggtgcaagta ctttatgcct ggaatcaata aaaaaagcag atttacctga
      781 ttcatatttg cttttagcaa agatatactt ttttaccaat aatagctatt ttgaaaaaac
      841 aatagaaaag tatatccggt taaccgatag ttctcaggga tatattctat atgcacgatg
      901 gtttataaga aaccagaaac aagatcaggc aatcagatat cttgaaaaag cacttctgca
      961 ttctcccaat gatcctgttg cattatatct tctgggcact attcagtatg caaataaaca
     1021 gtatgatgat gctattataa cattccacag agcttttgcc aactatttcc cgctcatgca
     1081 aatacacaga caattacagg aaatatatag cgagatacat ctgcctgaac gtgctgcaaa
     1141 gcaccaggca atccttgaag ccataaagca atttgatgat tcaacaaatg gagagtaact
     1201 atgaaaaagc ttatgattat ggtatctgcc cttgtatttt ttactgcatg caagtcttta
     1261 gatattcaaa cagttcagca tgctccattt gctaatgatt caatttttat caaagacgtg
     1321 cgcattcaca atgagctata tagcactata aaagattcat tagtttctca tattgttttt
     1381 catctgcaaa agtataatct caaagctgct tcatatttta atcccatata tcgcgaacag
     1441 gatttcaaat atacagctac tgttgatatt ttcatcacta aaactggcga tattttcaat
     1501 caggtggaaa atgcttcact ttttatattc atcaacgatg catcaatgca tattgctagc
     1561 atacggatta tttcacaaag ctgtcgctta caggaaagtt ttgaccaggc actgatagca
     1621 aaagctatcg ctaaagaaat tgacgctatg gttgttaaaa aatgaaaaag tttttttgca
     1681 tttttgcagt ttacatcatt ggaacctgtt ctgcatggag tcaggaaaca ctgacattgc
     1741 acaatgcatg cgagttagca ttgaaaaaca gtgtaaagct tgaaaggcta aaagcaaaga
     1801 ttcaatcatc acgtatgatt gtacacgaac gatggcgtga gttgcttcct aatgtttctt
     1861 ttcaatattc aaaaagcgat actgttgcca ttagagaaga tgatacacgt tttcaatcgc
     1921 ttgcatgtac agtacaatat gatattttta caaaccaaca atcgttaatt gcatattcaa
     1981 tagcacagat tgaatctatg cttgcaaatg aagaatatac catagaaaaa aataatatta
     2041 ttctttcaac taaacaggca tactttgctc tacaaaaaaa gagaaaatca attgaaatct
     2101 atcagcttct tttaaaaagc ctccagttac aaaaaaacat cattactgca caacaacaat
     2161 taggtatggc cacagagctt gagctgatac aggttgatgg gaaaattgct gaagcgcagt
     2221 acaatattct cagcgcacaa aatcaatatg ccaattcatt gcaggatttt gctaccactt
     2281 taggtattaa ctatgcttct atacaattgc cagagcagga atatactgag ctacaaaaca
     2341 tcactatggc aagtaaagaa aacctgatat cattatcaat gcaaaatcgc aatgaactta
     2401 agaaaagtta ttatacactt ataaaaactc aaaaagaata ccagctggca cagtattact
     2461 atctaccacg aattcaactt tttgcaagtt acggttatag cggtacagaa tttcccatga
     2521 ataaaaaaat gtggaatgtt ggtctgtcag taacaagttt actttttgga aacacagctt
     2581 ccctgagcaa ttcatatgga aaaaaagaca atggtaatac tacagacata aagtccagtg
     2641 catctgttgc tgtctatgag aatccatcgt ttatacgatc tataattgat gctgaagcag
     2701 catacactga agcgcagaag tatcatcagc aacttattca gataatacgt actgatgttt
     2761 caaaagctta tgatactgtg atggagtctt tgaaaaaagt tgaaatagcc aggcaaaccg
     2821 tactgcttct tgaaaaacaa tcttcaattg aaaatgagca ggctcgttta ggtgatatca
     2881 cccgctatga tgtgttaaaa acactggttg aactttcgca ggcacgtctc aggctgcagg
     2941 aaacaataac tgatgtcttt gtatcaatgg cagcattaga aaatgccgtt ggactgccta
     3001 ttgaatccat attttaaaaa tatgagggag atatataatt gattatgaac ttttttacgt
     3061 tactcagaga aaataaaaga aaaatttatt tattagtttt actgtccgta attatctcaa
     3121 cagcatatgc catactttcg ttatctttgt ataacgatac aataattcag gaaatgc
//
LOCUS       DAOSZP010000227         3172 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_24084_length_3172_cov_2.451396, whole genome shotgun sequence.
ACCESSION   DAOSZP010000227 DAOSZP010000000
VERSION     DAOSZP010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3172
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24084_length_3172_cov_2.451396"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..687)
                     /locus_tag="PK348_05880"
     CDS             complement(<1..687)
                     /locus_tag="PK348_05880"
                     /inference="COORDINATES: protein motif:HMM:NF013316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HXK65777.1"
                     /translation="MALKTGKNIELVAPAGNFEKLQAAVHYGAHAVYFGGQQYNLRAT
                     TKNFSIPEIEQAVAYAHTHNVKAIFLLNAFLHESDIPVVTEYIQHIKHIPFDAIMVSD
                     PGMSLLVKEHLNVPLHLSTQMSTLNHLAAKFWASHGISRIVLARETTLEEIKQIREHT
                     DIELEIFCHGALCISYSGRCLLSRYLSGRDGNQGNCSHPCRWKYSLVEEKRPGNHLDI
                     IEYSCGTEILS"
     gene            complement(659..1669)
                     /gene="mltG"
                     /locus_tag="PK348_05885"
     CDS             complement(659..1669)
                     /gene="mltG"
                     /locus_tag="PK348_05885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HXK65778.1"
                     /translation="MKEIKAAALSFIILFIIFAIVFWFFNSPPYTMKGDTITVRPGMN
                     IHQVAYFLKSKNIIKSASFFYILGRVLNATDVKAGNYRIYDGESSFSILIKLTRGKFV
                     TKRITIPEGYTIYDIARVLAANDICTEGDFLYWAQSPSFLASLGIRAPSAEGYLFPDT
                     YVIPENTDPRDIIIRLVNQTKKVIHDIQESIEVQYSNLHQILTVASLVEKEAKINSER
                     ILIAAVFYNRLKLKMKLDCDSTIQYGLKKFGKRLTYDDLDSDTPYNTYKYTGLPPTPI
                     CNPGKRSILAALNPAQVPYLFFVSRNDGSHYFSKTLSEHNRAVDFYQKGIRNGFKDRQ
                     KY"
     gene            1777..>3172
                     /locus_tag="PK348_05890"
     CDS             1777..>3172
                     /locus_tag="PK348_05890"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXK65779.1"
                     /translation="MCRKNIAILLFLFCCVVLYGQEPVKLQQKNAIYYNKKGWERLET
                     GNAYSAIVAFQSALAQNPKYKEALLGLGYAYLHTEGYTESVKLFDAVLKLYPNNEQAL
                     LGMAKAYTGLGRYREAMKIYDTLNELSYTNNDSRYGMAYLYYKMGNMLWAKRMLNRIL
                     QNDPYYYDALLLLADIKAKDKRFDDAIDLVKKAIDAKETYPDAFVKLAELLYLKYYYS
                     GDKAYLQQAVDELQKALIINKTHLQANRDMGYLLYIQGDYQNAISYYEAIVTHYPHIA
                     SNEILYNLGLMYEYKGDSNQSLTYYQKALSKDNLNSLIAMKMRQLCLLEHIKIGHPVR
                     IQSSNDDFSSSLELRKKGFPDLSMLFVRLSLALNPNNTTARQELMRIYETLGYFEFYY
                     DEIKKMHTLNPTQKTQDMMSIAVMKRRDKLFFRLGYDKEELPRDVPLVLVLDFTPTVA
                     VPAFADAGAVIANSI"
BASE COUNT         1016 a          590 c          585 g          981 t
ORIGIN      
        1 ggaaagaatt tcagtgccgc aggaatattc aatgatgtct aaatgattgc cgggccgttt
       61 ttcttcaacc agtgaatact tccatcgaca gggatgtgaa caatttccct gattgccatc
      121 acggcctgaa agatagcggc tgagcaaaca cctacccgaa tacgaaatgc acagtgctcc
      181 atggcaaaat atttccagtt caatatcagt gtgctcacgt atttgtttaa tttcttcaag
      241 agtggtttca cgtgcaagca caatacgtga tataccatgg cttgcccaga attttgctgc
      301 caggtgattt aacgttgaca tctgcgttga aaggtgcagg ggaacattga gatgctcttt
      361 gacaagtaaa gacatacccg gatcagaaac catgattgca tcaaatggaa tatgttttat
      421 atgctgaata tattctgtaa caacaggtat atccgactca tgcaaaaaag cattcagtaa
      481 aaatattgcc tttacgttat gagtgtgggc atacgctacc gcctgttcta tttcaggtat
      541 tgaaaaattt tttgtagttg ctctcagatt atattgctga cccccaaaat acacagcatg
      601 tgcaccatag tgtactgctg cctgcaattt ttcaaaattg ccagcaggtg caaccagttc
      661 aatatttttg cctgtcttta aagccattcc ttataccttt ttgataaaaa tcaacagccc
      721 tgttatgctc agataacgtt tttgaaaaat agtgcgaacc atcattcctg gaaacaaaaa
      781 acagatatgg cacctgtgcc ggatttaacg cagcaagtat tgatcgtttc cctggattgc
      841 atattggcgt tggtggtaat cccgtatatt tataggtatt atacggagta tctgaatcta
      901 aatcatcata ggtaagacgt ttaccaaatt ttttcagtcc atactggatg gttgaatcgc
      961 aatccaattt catttttaat ttaagcctgt tataaaatac tgcagcaata agaatacgct
     1021 cactgttaat ctttgcttcc ttttcaacta atgatgctac agttagtatt tgatgaaggt
     1081 tagaatactg tacctctatt gattcctgaa tatcatggat cacttttttt gtctggttta
     1141 ccaggcgaat tattatatca cggggatcag tattctcagg tattacgtac gtatcaggaa
     1201 ataaatatcc ttcagcactt ggcgcacgta ttcctaaaga tgctaaaaat gatggtgact
     1261 gagcccagta taaaaaatca ccttctgtac agatatcatt tgcggccaat actctggcaa
     1321 tatcatatat ggtgtatcct tcaggaattg ttattctttt tgttacaaat ttacctctgg
     1381 ttagttttat caatattgaa aagcttgatt caccatcata tatgcgatag ttacctgctt
     1441 taacatcagt agcattaagg acgcgcccca ggatgtagaa aaacgatgca ctcttgataa
     1501 tattcttgga tttaaggaaa tatgcaacct gatgaatgtt catacctggt cttacggtaa
     1561 tagtatcacc cttcatggtg tagggagggc tgttaaaaaa ccagaatact attgcgaaaa
     1621 taataaatag tataataaac gataatgcag ctgcttttat ttctttcatt attttcctga
     1681 tttgcaccaa aataaatatt ttgttgactt tacaagctaa gttgctatct aataactttt
     1741 gtttttacta ttactatttt atcaattaaa aaactaatgt gcagaaaaaa tatcgctata
     1801 ctacttttcc ttttctgttg tgttgttctt tacggacagg aaccagtaaa gctgcagcag
     1861 aagaatgcta tctattacaa taaaaaaggc tgggaaagac ttgaaactgg caatgcatat
     1921 tctgcaattg ttgcatttca aagtgccctt gcccaaaacc caaaatataa agaagcatta
     1981 ttgggattag gttacgctta cctgcacact gaaggctata ctgaatcagt taaattattt
     2041 gatgcggtgc ttaaattgta tcccaataat gagcaggcac tattagggat ggcaaaagca
     2101 tatactggtt taggccgcta tcgtgaagca atgaaaatct acgatacatt aaatgagcta
     2161 tcgtacacca ataatgattc acgctatggc atggcatatc tatactataa aatgggtaac
     2221 atgctgtggg caaaacgcat gcttaaccga attttgcaaa atgatccata ttattacgat
     2281 gcactgttac tcttggctga tataaaggca aaagacaaac gctttgacga tgccattgat
     2341 ctggtaaaaa aagccataga tgcaaaagaa acataccctg atgcttttgt caaattggct
     2401 gagctactgt atcttaaata ttattattct ggcgataaag catatttaca acaagcagtt
     2461 gatgaattac agaaagcgtt aattataaac aaaacacatc tgcaggcaaa tcgcgatatg
     2521 ggatatttgt tgtatattca gggggattat caaaatgcaa tttcgtatta tgaggcgata
     2581 gttactcatt atccacatat tgccagcaat gaaatcctgt ataatctagg cttaatgtat
     2641 gaatacaaag gcgatagtaa ccagtctcta acatattacc agaaagcact atcaaaagac
     2701 aaccttaata gcctcattgc catgaaaatg cgacagcttt gcttactgga acacattaag
     2761 ataggccatc ctgtacgaat acagagcagt aatgatgatt ttagctcatc actggaatta
     2821 cgaaaaaaag gatttcccga tttatcaatg ttgtttgtcc gtttatcatt ggcactcaat
     2881 cccaataata ccacggcacg tcaggagtta atgcgtatat atgaaactct aggctatttt
     2941 gagttttact atgatgaaat aaaaaaaatg catacattaa accccacaca aaaaacacag
     3001 gatatgatga gtattgccgt aatgaagcga agggacaagc tgtttttccg tttaggctac
     3061 gataaagaag aattgccacg agatgtgcca ctggttctgg ttcttgattt tacacctact
     3121 gttgcagtac ctgcatttgc cgatgcggga gctgtaatag ccaattcaat ca
//
LOCUS       DAOSZP010000228         3166 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_24137_length_3166_cov_2.777885, whole genome shotgun sequence.
ACCESSION   DAOSZP010000228 DAOSZP010000000
VERSION     DAOSZP010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3166
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24137_length_3166_cov_2.777885"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..493)
                     /locus_tag="PK348_05895"
     CDS             complement(<1..493)
                     /locus_tag="PK348_05895"
                     /inference="COORDINATES: protein motif:HMM:NF024313.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXK65780.1"
                     /translation="MKGYWSIVWKRFKQNKLAVVGLCIVIFLFVIALCAPLIANNKPY
                     ILIYKDKLYFPILFEYPEFSGLDFKKIEGFKIFPPIPYSYSEYDLNAIVLPPSAKHIL
                     GTDEQGRDLAARMLYGTRVSIFVGFVAVFIYVSIGIIIGALAGFYGGWVDIIISRIIE
                     IVMC"
     gene            complement(490..1491)
                     /locus_tag="PK348_05900"
     CDS             complement(490..1491)
                     /locus_tag="PK348_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXK65781.1"
                     /translation="MKYYILKRILIIIPTFIGITFITYLMIRLAPGDYTSLRAGVEGE
                     LKAGALSKEIFEQEKKLYGLDKPVIVGYAEWFYKTVKLDFGTSRKDGRKVISHIADAL
                     PITLALNILSIIIVYIISIPLGIYSSIKKDTVQDRIISLTLFLLYSLPSFWVALLLLK
                     YLSGGDYLNLFPLGGLYSDWFDQLNLFQKAMDILWHLILPVVTLTYGGFAFLSRYTRA
                     TMLDVINQQYIITARAKGLSEQKVLFVHAFRNSLIPLLTLMATMLPGLLGGSVIVESI
                     FSIPGMGMLAFESILSRDIPIIMAITSISAFLTLIGIFLADIMYAIADPRIRLEAKQ"
     gene            complement(1494..2993)
                     /locus_tag="PK348_05905"
     CDS             complement(1494..2993)
                     /locus_tag="PK348_05905"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HXK65782.1"
                     /translation="MRDPYTLYIHISAEPGHLNPITSNEAIASSINSYIYETLLDRDY
                     DTLELKPQLATRWEISSDKLHYRFYLKENIYWSDGKPFTADDIVYSFKIIKDPRVANA
                     PLKVYYIDVKSVKKISPYIVEFTYAKPYFLALEICGSIPIVPKHIFDDGTDFNTHKNN
                     RFPIGTGPYKFVTWNTGKTIELVVNDRYWGPKPDIKRIVYKIIPEPNVALQMLKKGEL
                     DVMALRPIQWVRQTNSKHFQESFYKFMYYQPYYNYIGWNARNPLFKDKNVRKALTMLI
                     NRQAILDSLLFGLGTVVTGPFYIHDKYYSHDIQPLPYDPVKAKEILHRAGWSDSNGDG
                     ILDKNGVAFKFTLTIASASKFAERLATILKEDFSKVGIQMDINRYEWAIFVNKVDKRD
                     FDAVTLGWSLSWEGDPYQLWHSSQVQAGSNFCYFINAEADEIIEKARKEFDVEKRIKM
                     YRRFHEIIHDEQPYTFLYCTPALVVVSKRFDNVIVHKRGLNYTEWKVKQ"
BASE COUNT         1043 a          591 c          532 g         1000 t
ORIGIN      
        1 aacacataac aatttctata atacgtgaga taataatatc tacccatcct ccataaaatc
       61 cagctaaggc accaataatt atacctatag atacataaat gaatactgct acaaatccaa
      121 caaagataga tacacgagtg ccatataaca tacgtgcagc caaatctcta ccctgttcgt
      181 cagttcctaa aatatgttta gcactgggtg gcagcacgat tgcattgagg tcatattcag
      241 aatatgaata tggtattggt ggaaatattt tgaatccctc gattttttta aaatctaaac
      301 ctgaaaattc aggatattca aacagaattg gaaaatatag tttatctttg taaattaaaa
      361 tataaggttt gttgttggca attaacggtg cacataatgc aattacaaat aaaaaaataa
      421 caatacagag gccaactaca gccagtttgt tttgtttaaa gcgtttccat acaattgacc
      481 agtagccttt cattgtttag cctccagtcg tatacgagga tcggcaattg catacataat
      541 atccgcaagg aaaataccaa tgagcgttaa aaaggccgaa attgaggtta tagccatgat
      601 aataggaata tctcgtgaaa gtatggattc gaatgccagc atacccattc caggtattga
      661 aaagatagat tctactataa cactgccacc taaaagacca ggtagcatgg ttgccataag
      721 tgtcaataat ggaattaaag aatttcggaa agcatgaaca aacaaaacct tttgttcgga
      781 tagtcctttt gcacgtgctg taataatata ttgctgatta ataacatcca gcatggttgc
      841 ccgtgtgtag cgtgatagaa atgcaaaacc accataggtt aatgttacta ctggcagaat
      901 taaatgccac agtatatcca ttgctttctg aaaaagattt aactgatcaa accagtctga
      961 atataaaccg cctaatggga ataaattcag ataatcccca ccactcaggt attttagcaa
     1021 caacaacgca acccagaatg aaggtaaaga ataaagcaaa aatagtgtga ggcttataat
     1081 tctgtcctgc actgtatctt tttttatcga agaataaatg cccaatggga tagagataat
     1141 atacactata atgatagaaa gaatgttcaa tgctaatgta attggcaatg catcggcaat
     1201 atgggatata actttacgcc catctttacg tgaagttcca aaatcaagtt ttactgtttt
     1261 ataaaaccat tctgcataac ccacaattac tggcttatca agcccataga gtttcttttc
     1321 ctgttcaaat atctcttttg aaagggctcc tgctttcaat tcaccttcta cacctgctcg
     1381 taaagaagta taatcgccag gtgccagccg tatcataaga tatgtaataa atgttatgcc
     1441 aataaatgtt ggtatgataa taagaattcg ttttaaaata tagtatttca tttttactgc
     1501 ttgaccttcc attcagtata attcaggcct cttttatgga caataacatt atcaaaccgt
     1561 ttgctgacaa ccaccagtgc tggagtacaa tataaaaatg tatagggttg ctcgtcatga
     1621 attatttcat ggaaacgcct gtacatctta atgcgctttt ctacatcaaa ctctttgcgg
     1681 gctttttcaa taatttcatc tgcttcggca ttaataaaat aacaaaaatt tgaccctgcc
     1741 tgtacctgtg atgaatgcca tagctggtag gggtcgcctt cccatgacaa tgaccagccc
     1801 agggtaactg catcaaaatc acgcttatcc actttattta caaaaatagc ccattcataa
     1861 cggtttatgt ccatctgaat gccaaccttt gaaaaatctt ctttcaagat agttgccagc
     1921 cgttcagcaa acttgcttgc tgaagcgata gtcaatgtaa atttgaaggc tacaccattt
     1981 ttatcaagga tgccatcccc attactatcg ctccagcctg ccctgtgcaa tatttcctta
     2041 gcttttacag gatcgtatgg caaaggctgt atatcatggc tataatattt atcatgaata
     2101 taaaatgggc cagtaacgac tgtccccaat ccaaatagca gactgtcaag aattgcctga
     2161 cggttgatta acatggtcag cgctttacga acatttttat ctttaaataa aggattgcgt
     2221 gcattccacc caatatagtt ataatatggc tgatagtaca taaatttata aaaagattcc
     2281 tgaaaatgct ttgaatttgt ctgccgtacc cactgtattg gcctcaatgc catgacatca
     2341 agctcgcctt tttttagcat ctgtagtgca acatttggtt cgggaattat tttatatact
     2401 atgcgcttta tatctggctt tggaccccag tatctatcgt tcacaactag ttcaatggtt
     2461 tttcctgtat tccatgttac aaatttataa ggtcctgtgc caatagggaa acggttgttt
     2521 ttatgagtat tgaaatcggt tccatcatca aatatatgtt tgggaacgat aggaatgctt
     2581 ccacagattt caagcgccag gaagtaaggc ttagcatacg taaattcaac aatatacgga
     2641 gaaatttttt ttacactttt tacatcaata taatacactt ttaacggtgc attagcaaca
     2701 cgtggatctt ttattatctt gaatgaatat acaatatcat cggctgtaaa aggttttcca
     2761 tcactccagt aaatgttttc tttaagataa aagcggtaat gcaatttatc cgatgaaatt
     2821 tcccatcttg tagccagttg tggttttaac tctaatgtat cataatccct atcaagcagt
     2881 gtttcatata tatatgaatt aattgaagaa gctatagctt catttgaagt tatggggtta
     2941 agatgccctg gttcagctga aatatgaata tataaagtat atggatcacg cactgattca
     3001 aagcgtgtgc aggaagacag gataataatt agtattatat aacatagtct tatataagat
     3061 ttcatcatat cactacaaca accttgttat gtaaagtgag ttgcttactg taactttctc
     3121 tgaaactaat aattaacgaa tgtattatat tgaattatgt ttctta
//
LOCUS       DAOSZP010000229         3160 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_24181_length_3160_cov_3.120773, whole genome shotgun sequence.
ACCESSION   DAOSZP010000229 DAOSZP010000000
VERSION     DAOSZP010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3160
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24181_length_3160_cov_3.120773"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..768)
                     /locus_tag="PK348_05910"
     CDS             complement(<1..768)
                     /locus_tag="PK348_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015905789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HXK65783.1"
                     /translation="MSNVHIIGSGMIRFNKYPDETVRSMAEKAIKLALEDAGIDKKDI
                     QAGFFSNTFWGMFSGQHSIRGQVIFRGMGIDKIPVVNVENACAGGSSALHLAYTGIKA
                     GMYDVAIAVGSEKITNPNKALSLGAYASCMDVENFEKHINMIIEVSKSFKITIPHDES
                     KPGEGRSVFMDAYAVGARWHMDRFGSTQHQLAVICSKNHLHGSLNPLSQYQHKMTVEE
                     VLADKLVAWPLTRAMCAPVGDGAAATIVCSEKFLKKFL"
     gene            complement(761..2005)
                     /locus_tag="PK348_05915"
     CDS             complement(761..2005)
                     /locus_tag="PK348_05915"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013259987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXK65784.1"
                     /translation="MLYTELNIELTDEHQHLKEQVHRFSMEVLRPASLELDKLPAEEV
                     VKKGSIYWDCMAKMYQNGYHTVLISDEYGGLGLDPLAVHIFWEELAYGSVGFAVSLGC
                     SCFAAFYATMLAEDKLIEKFVIPFVNCKDASVMSCWGITEPDHGSDNLAPRTSFFKDA
                     SLTQQVKAHKKNGEWIISGQKSAWISNGPVASNCALFLNLDPSKGLSGGGICLIDLNQ
                     DGVTRGKPLDKMGQRELPQGEIFFDNVVCPEEYMIVDPESYEAMTELTLGHANATMGA
                     YFTGLAQAAYDMTLQYCTERIQGGKRLCDHQWVQKKLFDMFVKVETARAFSRAAMIYN
                     MNTTPPDVKYSIASKVYCTEAAFQVTSDAIQLFGGYGLSKEFPIEKLFRDARAGMIED
                     GSNDTLSMTAGNIIIKEKLGDE"
     gene            complement(2044..>3160)
                     /locus_tag="PK348_05920"
     CDS             complement(2044..>3160)
                     /locus_tag="PK348_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013259986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HXK65785.1"
                     /translation="WDTVDQMIMDVRHPMQIIYTSGTTGDPKGVAIRNNRTGLFNILN
                     SIVWRYQKDDILYNGLSLTHGNAQAVTLFPALSMGITAVFSPRFTKSRIWDICRKYGC
                     TSFSLLGGMMAGIYNEPEKPDDADNPVKVVISAGTPTTIWEAFEKRFNVKILEWYGSV
                     EGGFAYKPIGKGPIGSFGKPVPGVMEFKIVDENDNEVPVGQTGELICRLLKGETKVDY
                     LGKPEESKEKTRGGWLRTGDMVHRDAEGWYYFDYRKGSELRRSGDFIQPEYIETVIGK
                     HPDVSEVSVYGVPAASGAPGESDLVAAIACFEGKSVDPKSFYEYLKKNLEANFIPSYL
                     QIVPEIPKTITEKALTRVLKEDFLKGLGTIYKYEDYK"
BASE COUNT          881 a          763 c          533 g          983 t
ORIGIN      
        1 cagaaatttt tttaaaaatt tctcagaaca tacaattgta gcagccgcac catcaccaac
       61 cggtgcacac atagcacgag tcagtggcca ggcaaccagc ttgtctgcca gtacttcttc
      121 tacggtcatt ttgtgttgat actgtgacag aggattgagt gatccatgta aatgattctt
      181 agagcatata actgctaact ggtgttgagt ggacccaaac ctgtccatat gccaccgcgc
      241 tccaacagca tatgcatcca taaatacgct acggccttca cctggtttgc tttcatcgtg
      301 aggtatggta attttaaatg atttacttac ttcgattatc atattgatat gcttctcaaa
      361 attttcaaca tccatacacg atgcataggc acccagggac agtgccttat taggattggt
      421 aattttttct gaaccaacag ctattgccac atcatacatg cctgctttta tgccagtata
      481 agctaaatgc aatgctgatg atccacctgc acacgcattc tctacattaa ctacaggaat
      541 tttatcaatt cccattcccc tgaaaattac ctgtcctcgt atggaatgct gtcctgaaaa
      601 catgccccag aatgtatttg aaaaaaatcc tgcctgaatg tcttttttat ctatgccagc
      661 atcttccagt gcaagcttta tagccttttc agccattgac ctgactgttt catcaggata
      721 cttgttaaac cgtatcatcc ctgagccaat tatgtgaaca ttactcatct cccaatttct
      781 ccttaataat aatatttcct gctgtcattg ataacgtgtc atttgagcca tcctcaatca
      841 tccctgcacg agcatccctg aacaattttt caatggggaa ttcttttgac agcccatagc
      901 cgccaaaaag ctggatggca tcactggtta cctgaaatgc tgcttcggta cagtatactt
      961 tggaggcaat ggaatattta acatcaggtg gtgtggtatt catattgtat atcatggcag
     1021 cgcgggaaaa tgctctggca gtctcaactt ttacaaacat atcgaataat tttttctgca
     1081 cccattgatg gtcacataat cgttttcctc cctgtatacg ctcagtacaa tattgtaatg
     1141 tcatatcata ggctgcttga gcaaggccag taaaataggc acccattgtt gcattagcat
     1201 gccccaatgt taattccgtc atggcttcat aactttctgg atctacgatc atgtactctt
     1261 cagggcagac aacattatca aaaaatatct cgccctgagg aagttccctc tgccccatct
     1321 tatcaagtgg tttgccccgt gtaaccccat cctgatttaa atcaataagg catataccac
     1381 ctccagacaa accttttgaa gggtccaggt tcagaaataa tgcacagttt gaagcaacag
     1441 gtccatttga aatccatgct gacttctgtc ctgaaatgat ccattcacca ttttttttat
     1501 gcgccttgac ctgttgtgtc aatgaggcat ctttgaaaaa actcgtccgt ggtgctaaat
     1561 tatcacttcc atgatcaggc tcagtaattc cccagcaact cataacgcta gcatccttac
     1621 aatttacaaa cgggataacg aatttttcta taagcttatc ctcagccagc atggtagcat
     1681 aaaatgcagc aaagcagcta cagcctaacg atactgcaaa tcccacactg ccgtatgcca
     1741 gctcttccca gaagatatga actgccaatg gatccagtcc caatccacca tattcatcag
     1801 aaatcaaaac agtatgatag ccattctggt acatcttagc catgcaatcc cagtaaattg
     1861 aacctttttt gacaacttct tcagccggaa gtttatccaa ttccagagaa gcaggtcgta
     1921 atacttccat tgaaaacctg tgaacctgct ctttaagatg ctgatgttca tctgttagtt
     1981 ctatattgag ttctgtatac aacatacgaa cctccataag tagatgtttt ccctatacga
     2041 ttattattta taatcttcat atttatatat tgtcccaagg ccttttaaaa agtcttcttt
     2101 aagaacccgt gttaaagctt tttctgttat tgttttggga atctctggaa caatctgcag
     2161 atatgaagga ataaaatttg cttccaggtt ttttttcaga tactcataaa atgactttgg
     2221 atcaactgat tttccttcaa agcaggcaat agcagcaaca agatcgcttt ccccaggagc
     2281 accggaagca gcaggaacac catataccga cacttctgat acatcggggt gtttgccaat
     2341 gactgtttca atatattctg gctgaatgaa atctccggac cttctaagct ctgacccttt
     2401 acgataatca aaataatacc agccttcggc atccctgtgt accatatcac cggttctcaa
     2461 ccaaccacca cgcgtctttt cttttgattc ttcaggcttg cccagataat caacttttgt
     2521 ttcacctttc aataatctgc atattaactc cccggtttgt cccaccggaa cttcattgtc
     2581 attttcatcc actatcttaa attccattac acctggtact ggtttgccaa aagatccaat
     2641 gggccctttc cctataggtt tatatgcaaa gccaccttca accgacccat accattcaag
     2701 tattttaaca ttgaagcgtt tttcaaatgc ttcccaaatt gttgtcggag tcccggcact
     2761 tatgaccact ttcacagggt tgtcagcatc atccggtttt tcgggctcat tataaattcc
     2821 tgccatcatc ccgcctaaaa gtgaaaatga cgtgcatcca tattttctac atatatccca
     2881 tattctgctt ttggtaaatc gggggctgaa tactgcagtt attcccatag agagtgctgg
     2941 aaatagagta actgcctgtg cattaccatg tgtcagtgat aacccattat acaggatgtc
     3001 atctttttga tagcgccaca caatactgtt taagatatta aacaatccag ttctgttatt
     3061 cctgattgct actccttttg ggtcaccggt agtacccgag gtatatataa tctgcattgg
     3121 gtgccgtaca tccattatca tctgatcaac tgtatcccag
//
LOCUS       DAOSZP010000230         3155 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_24225_length_3155_cov_2.618387, whole genome shotgun sequence.
ACCESSION   DAOSZP010000230 DAOSZP010000000
VERSION     DAOSZP010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3155
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24225_length_3155_cov_2.618387"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(96..1538)
                     /gene="purF"
                     /locus_tag="PK348_05925"
     CDS             complement(96..1538)
                     /gene="purF"
                     /locus_tag="PK348_05925"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002773918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HXK65786.1"
                     /translation="MKTEYEYLDGKPREECGIFGIYNHPKAANLTYLGLYNLQHRGQE
                     SSGIASSDGYHIYRYAGMGKVVDVFTEEHINNLKGNIAIGHNRYSTTGSSFLRNAQPL
                     RAHSVLGPIVLAHNGNLVNYAMLRRALEKEGAIFQTSIDSEVIVHLMARSGLTTFLDA
                     LIYALKAIKGAYSLLVMNYNTMYAIRDPYGFRPLVLGKLGDSIVIASETCAFDIIDAE
                     YIREIEPGEMLEISPTGIKSYFPFASVKNSLCVFEYIYFSRPDSIIFGQSVHEMRLRM
                     GAMLAKQAPVDADIVVPIPDSSVCAALGYARASGIPYEMGMIRSHYIGRTFIEPEQRI
                     RDFGAKIKFNIVRSAVEGKRIVVVDDSIMRGTTMRKIIKMFRKGGAKEIHVRISAPPT
                     RFPCFYGIDIPTRSELIASSHTIEEIQKYLRVDSLAYMTVESMLKALDVHHGKFCTAC
                     FDGNYPVEFQEADDYQKCLFDDTVACGEYY"
     gene            2159..3094
                     /locus_tag="PK348_05930"
     CDS             2159..3094
                     /locus_tag="PK348_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013537555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXK65787.1"
                     /translation="MKICITGGAGFIGSHIAEECVKKGYEVIVFDNFSTGKKENLSFV
                     KNFPGSSLKIVKGDVADYKAVSKAVKGCNAIFHEAAVASVQKSIENPPATFESNAKGT
                     LNVLEASRNNGVTKVIFACSAAIFGDHPEIPKREDSPVLPKSPYGADKYISEVYLRLY
                     NELYNLTTVSLRYFNVFGPRQDPSSPYSGVISIFTTRIAQGKDITVFGDGKQYRDFIF
                     VKDVVQANMLALEKIHSGFHYYNVGYGKATDLNTLITILQKIYQKEITVNYADARPGD
                     IKESVSDPTKIIRELGFSPNYTVEEGLRILVESLL"
BASE COUNT          983 a          570 c          632 g          970 t
ORIGIN      
        1 ccaacctgcg gttttggaga ccgctgctct accaattgag ctagtcacct gtatacgtaa
       61 caaagaaaaa tttgtaacaa ttatgtcaag cgtttttaat aatactcccc acatgcaacc
      121 gtgtcatcaa aaaggcattt ctggtaatca tcagcttctt gaaattcaac aggataattg
      181 ccatcaaagc acgctgtgca aaattttccg tgatgcacgt ctaaggcttt caacatgctt
      241 tcaaccgtca tgtacgcaag tgagtccaca cgcaggtatt tttgaatttc ctcaatggta
      301 tgcgatgatg caataagttc tgaacgtgtg ggtatgtcta tgccgtaaaa acaggggaaa
      361 cgtgttggtg gtgctgagat acgaacatgg atttcctttg cgccgccttt tctgaacatc
      421 ttgataatct ttcgcatggt ggttccacgc ataattgaat cgtcaacaac aacgatgcgt
      481 tttccctcaa ctgctgaacg gacaatgtta aattttattt tagcgccaaa gtcgcgaatg
      541 cgctgttcgg gttcaataaa agtacggcca atgtaatggc tgcgtatcat gcccatttca
      601 tagggtatac cgcttgcacg agcataccct aacgctgcac acaccgatga atcaggaatg
      661 ggaacaacga tatcagcatc aacaggtgct tgctttgcaa gcatagcccc cattctaaga
      721 cgcatctcat gtaccgattg gccaaagatg atgctatcgg gtcgtgaaaa atagatatat
      781 tcaaacacac acagagaatt tttaacactt gcaaatggga agtatgactt tatgccggta
      841 ggtgatattt caagcatttc cccgggttct atctcccgaa tatattctgc atcaatgata
      901 tcaaaagcac aggtttcaga cgctatgaca attgaatcac ccaatttacc cagcaccagc
      961 ggtctaaacc cgtatggatc acggattgca tacattgtgt tgtaattcat gacaagcagt
     1021 gaataggcgc cttttattgc tttgagcgca taaatgagtg catcaagaaa tgtagtaaga
     1081 cctgagcgcg ccattaaatg cactataact tcactatcaa tggatgtttg aaaaatggct
     1141 ccttcttttt caagggcacg ccttagcatt gcgtagttta ccaggttgcc attatgtgcc
     1201 agcactatgg ggcccagtac tgagtgtgct cgcaaaggtt gtgcattgcg taaaaatgat
     1261 gagccggtag ttgaatagcg gttgtggcct atggctatgt ttcctttgag gttattgata
     1321 tgctcttcgg taaatacatc gacaactttt cccattcctg cgtaacgata gatatggtat
     1381 ccatcggatg aagcaatgcc gcttgattcc tgccctcgat gttgcaggtt gtaaaggcct
     1441 aaataggtaa gatttgctgc ttttgggtga ttataaatgc caaaaatacc acattcttca
     1501 cgcggtttac catccaaata ctcatattct gttttcataa ttatcctgaa aaatagcctg
     1561 gttagatttt tacgatttag accagtagta tcctttgtaa atgaagcttg tatagatatt
     1621 aagaataata aagttaattc caataaccaa tataatggta ttttgtataa aatcaataaa
     1681 aatttgctat ttgggcgata gtaactgggc aaatgctctt tatgtggggt tccccaaaat
     1741 tgttcatagg caatatcttg cacatatttg ctgataaaat gcaatcatat tgatacaatt
     1801 ctgcaatgtt gtgtgtcaaa attactaata tggacgttgt gaacttttta tcgaaatgac
     1861 tttttaaaac gcagcaatgt aataaatatg cttgatattg tattgaggtt ttgatactgt
     1921 aatttagtta tttattgtgg tattgataat aactgaatag tgtagttacg ttacagtttt
     1981 ccaacaagat tcgatacaat ctatactatg taagggaact tctaaaaaca agttttaagg
     2041 atgttcccat gaaaataaag taatatgatt tttcttttag cacttttttt gttgcagttt
     2101 ggacaaataa agtgcaaaaa gagttatagt taaaatagat ttttaagggg aaattataat
     2161 gaaaatctgt attaccggtg gtgctgggtt tattggctca cacattgcag aagaatgtgt
     2221 taaaaaaggg tatgaagtga tagtctttga taatttttcg actggtaaaa aagaaaacct
     2281 ttcatttgta aaaaacttcc caggttcaag cttgaagata gtaaaaggtg atgtggctga
     2341 ttataaagca gtttctaaag ccgtaaaagg ttgtaatgcg atattccatg aagctgcggt
     2401 tgcctcggta caaaaatcca ttgaaaatcc ccccgcaaca tttgaatcaa atgcaaaagg
     2461 gacattgaat gtgcttgaag caagcaggaa taatggagta acaaaagtga tatttgcctg
     2521 ttcagcggct atctttgggg atcatcctga aataccaaag cgtgaagatt caccagtttt
     2581 accaaaatca ccctatggtg ctgacaaata tatttcagag gtttatcttc gcttatacaa
     2641 tgagttatat aaccttacaa cggtgtcatt gcgctatttt aatgtatttg gaccccgtca
     2701 ggatccatca tccccgtatt ctggcgttat ttcaattttt acaaccagaa ttgcccaggg
     2761 taaagatatc acagtatttg gggatgggaa acaatacagg gattttattt ttgtaaagga
     2821 tgttgtgcag gcaaatatgc tggcactgga aaaaatacat tcaggttttc attattataa
     2881 tgttggatat ggcaaggcta cagatttgaa tacgcttata acaatactac aaaaaatata
     2941 tcagaaagaa attacggtta attatgccga tgcgcgacca ggggatatta aagaatcagt
     3001 gagtgatcca acaaaaatta ttcgcgaatt ggggttttct cccaattaca ccgttgaaga
     3061 aggattgcgg atacttgttg aatcactact ttagtatgta ctcattagtt taaacaaaac
     3121 actgatgcca ttacagcata gtacaccatt ataaa
//
LOCUS       DAOSZP010000231         3136 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_24390_length_3136_cov_2.706913, whole genome shotgun sequence.
ACCESSION   DAOSZP010000231 DAOSZP010000000
VERSION     DAOSZP010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3136
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24390_length_3136_cov_2.706913"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2306
                     /locus_tag="PK348_05935"
     CDS             <1..2306
                     /locus_tag="PK348_05935"
                     /inference="COORDINATES: protein motif:HMM:TIGR03936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HXK65788.1"
                     /translation="LYEVGMSNNGIQILYSAVNALKGAACERVFAVERDFEKVLKESG
                     TPLYTLETFTPLHELDMVGFNAACELLYTNILQVLELGNIPFFAKDRGDSYPLIVIGG
                     EAVSNPAPLLPFADAVFAGDGEEAIVEIVNLLIEKKTKGLSKDEVLAKLANIEGVLCS
                     RDVEFTYEGHTLKAIKAPDVYKRVAHNFHGYEATRPVVPSIRSVQDRVSIQLDRGCKN
                     LCKFCHAGYWELPYRKVPAKSIADTILDTVQHTGFSDVSLHSLSVGDYSDIVELLNYI
                     VPPLVEQGISISLPSLRVDLQTLDIIESISDLKKTSLTFAIESASDMLRYKAYKRLTV
                     EEIMNIITVLSQRGWQRIKLYFMIGLPGCEQVDEAADIVAFMRQACSINKKIQYNVTI
                     SPFVPKPHTPFQYEKQYDETYCLEVIRTIKRSVPKSVTIKNHDVYASLIEGVFARGDS
                     LLSNALVGAYHNGARLDLWGEHFDFTIWDNALQKTAGNWRRYLEKRPSQELSPWHVIK
                     TRYDSLIHKKMHSSWDGKTFPAGFKKVKLNKDAFKEAARSFEKRYKAVATLRVKYAKK
                     GKAQFLGHIDCMEIFKRALRMAQVPVAYTQGYNKHERLVTGPALPLGYESQCEFVDVE
                     LFQQCSDTVVRNFGTLFPDGFEIISAYHHTDTTLPLQSIAYSRYSITFLDGRLRDMFA
                     TKLKNKTDIVKKTKKGLKNLAFDDVVVEFQIHDDSVELLIPVGEGSIRPDNLIADWCE
                     VNNPLAVAAISKTAVYYMDNGAFVEFI"
     gene            2328..3092
                     /locus_tag="PK348_05940"
     CDS             2328..3092
                     /locus_tag="PK348_05940"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXK65789.1"
                     /translation="MGSLSYNLDGKVAIVTGGTRGIGLAIAQELLRQNAKVVICARKQ
                     EGLDKAKEILKGGDNLVTCVAHIAKEEDVNSLVDTTVKAFSKIDILINNVGMNLPTGS
                     LVDVELSAWQKIIDSNLTGTFLVSKKVGAIMRQQKSGKIVSISSIAGRLASPGMNVYG
                     IAKAGIEMMTKNLALELAPYNVQVNAVAPGMVRTDFSKPFWSNDAIHEMIVKNIPIGR
                     IAEIDEVVHPVLFLASDGARYITGQTLLIDGGASIV"
BASE COUNT          936 a          577 c          743 g          880 t
ORIGIN      
        1 acctgtatga ggttggcatg tccaataacg gtattcagat tttgtacagt gcagtcaatg
       61 cactgaaagg tgcagcatgt gaacgtgtgt ttgctgtgga gcgtgatttt gaaaaagttt
      121 tgaaagaatc ggggacacca ctgtacactc ttgaaacatt tacacctctg catgagcttg
      181 atatggtagg cttcaatgca gcatgtgaat tactgtacac caatatactt caggtgctgg
      241 aactgggcaa catcccattt tttgcaaagg acaggggcga tagttatccg ttaatagtca
      301 ttggcgggga agcagtaagc aatccagcac cactattgcc atttgctgat gcagtctttg
      361 ccggtgatgg tgaagaggcc atagtggaaa ttgtaaacct tcttattgaa aaaaagacaa
      421 aaggtttatc aaaagatgag gtgcttgcaa agttggcaaa tatcgaaggg gtgttatgca
      481 gccgcgatgt tgagttcacc tatgaaggtc atacattaaa agcaattaaa gcaccagacg
      541 tgtataaaag agttgcacac aattttcatg gatatgaagc tacacgacca gtggtgccat
      601 ccatacgcag tgtgcaggac agggtttcca ttcagttaga cagaggctgc aagaacctgt
      661 gcaagttttg ccatgcaggc tactgggagc ttccctaccg aaaggtacct gctaaatcaa
      721 tagctgacac aatacttgat accgtgcagc atactgggtt tagcgatgta tcgctgcact
      781 cactgagtgt gggagattat tctgatatag tggagctttt gaattatatt gttccgccac
      841 tggtagagca gggtattagt atatcgcttc cttcgctgag ggtggattta caaacgcttg
      901 atattattga atcaatatct gatttgaaaa aaacatcgct gacttttgct attgagtcag
      961 cttcggatat gctgcgttat aaggcgtaca agcgtcttac cgttgaagaa atcatgaata
     1021 ttatcactgt gttgtcccag cgtgggtggc agcgcataaa gctatacttt atgattggcc
     1081 tgcctggttg tgagcaggta gatgaagcag ccgatattgt tgcattcatg aggcaggcat
     1141 gttcaatcaa taaaaaaata cagtacaatg ttactatctc gccgtttgta ccaaaaccgc
     1201 atacgccatt ccagtatgaa aagcagtatg atgaaaccta ttgccttgag gtcatacgca
     1261 caattaaacg cagtgtacca aaatcggtaa caataaaaaa tcatgatgtc tatgcttctt
     1321 taattgaagg cgtgtttgca cgtggcgata gtttactttc aaatgccctt gttggagcat
     1381 accacaatgg agctcgactg gatttatggg gtgagcattt tgattttact atatgggaca
     1441 atgcattgca aaagactgct ggtaactggc gccgttattt agaaaaaagg ccatcacagg
     1501 aactatcgcc ctggcatgtt attaaaacac gctacgattc ccttatacac aaaaagatgc
     1561 attcaagctg ggatggcaaa acatttcctg ctggttttaa aaaagtaaag cttaataaag
     1621 atgcatttaa agaagctgcc cggtcatttg aaaaacggta taaagccgtc gcaaccctgc
     1681 gtgtgaagta tgctaaaaag gggaaagcac aatttttagg gcacatagat tgcatggaaa
     1741 ttttcaagcg agcactgcgt atggcccagg ttccagttgc ctatacgcag ggatataata
     1801 aacatgaacg tctggttacc gggcctgcat tgccgctggg ctatgaaagc cagtgtgagt
     1861 ttgttgatgt tgaactcttt cagcaatgca gtgatacagt agtacgtaat tttggaacat
     1921 tatttcctga cgggtttgaa attatatcag cctatcatca caccgacaca acacttcccc
     1981 tgcaatccat tgcatattcc cgctatagta taacctttct tgatggcagg ctgcgtgata
     2041 tgtttgccac caaattaaag aacaaaacag atatagttaa gaagacaaaa aaaggattga
     2101 agaatttagc ttttgatgat gttgttgtag agtttcagat acatgatgac agtgttgaac
     2161 tgctcatccc ggtgggtgaa ggttcaatac ggcctgataa tctcattgct gactggtgtg
     2221 aggtaaacaa tccactagca gtggcagcta ttagtaaaac tgctgtatat tatatggata
     2281 atggagcttt tgttgagttc atataacttt caaaaaggag gctttgtatg ggttcgcttt
     2341 cttataattt agatggcaag gttgcgattg taaccggtgg cactcgtggt attgggcttg
     2401 ctattgccca ggagctgtta cgccagaatg caaaagtagt gatttgtgca cgcaagcagg
     2461 aggggctgga taaggcaaaa gaaatcctta agggtggcga taaccttgta acctgtgttg
     2521 ctcatattgc taaagaagag gatgtgaata gcctggtgga cacaacagta aaagcttttt
     2581 caaaaattga tatattaatt aataatgttg gcatgaacct gccaacgggg agccttgttg
     2641 atgttgaact atcggcatgg caaaagatta ttgattcaaa ccttaccggc acatttcttg
     2701 tgtcaaagaa ggttggtgca atcatgcgcc agcagaagag cggaaaaatt gtcagcatat
     2761 catcaattgc aggaaggtta gcttcaccag gtatgaatgt gtacggcatt gcaaaagccg
     2821 gcattgagat gatgacaaaa aatctggcgc tggagcttgc accatataat gtacaggtaa
     2881 atgcggttgc acccggaatg gtgcgaactg atttcagcaa gccattctgg tcaaatgacg
     2941 ccattcatga aatgatagta aaaaatatac ctataggaag aattgccgaa attgacgaag
     3001 ttgtgcatcc tgttcttttc ttagcatcag atggagcgcg atatattacc gggcaaacac
     3061 tcttaattga tggtggtgct tcaatagtgt gatcaatcta tcaggagtat cgtaccatta
     3121 atttttaccg catagt
//
LOCUS       DAOSZP010000232         3135 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_24401_length_3135_cov_3.512338, whole genome shotgun sequence.
ACCESSION   DAOSZP010000232 DAOSZP010000000
VERSION     DAOSZP010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3135
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24401_length_3135_cov_3.512338"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            32..760
                     /locus_tag="PK348_05945"
     CDS             32..760
                     /locus_tag="PK348_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000345490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="motility protein A"
                     /protein_id="HXK65790.1"
                     /translation="MGIIFAKGNPLLFFDIPSVFITIGGSFCALLISNPLSKVLGIWK
                     VAKHAFFPPKYNPNELIITLVSFSEKARREGLLALEDDLDELDDQFLRKGIQLVVDGT
                     DPELVRRIMETELENIMARHDEGKKLFDDWATYAPAFGMIGTLLGLVLMLVNIEDKSA
                     IGPGMSAALITTFYGAIMAYLVLYPISSKLGFLNNQEVLMKLIMIEGTLSIQSGDNPR
                     IVKDKLVSFLDPRLREAITSEVGD"
     gene            760..1539
                     /locus_tag="PK348_05950"
     CDS             760..1539
                     /locus_tag="PK348_05950"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HXK65791.1"
                     /translation="MPKRKKMELPPPPPWLTSWGDLNTLLLTFFVMMFDISPIIGQDF
                     NLVLSSFKGSLGMMQGGYSLARGRMEEIGLNMLNLPSSEQGRALAKMLKRAVEAFKPE
                     IQAKKVRVREDERGLVITLSSDAYFEPGSAKLKDDIIPILKKVALIIKSMPNYVRIEG
                     HTDNRPVPPRGLEEGYATNWELSSARAVNVLRYLIEETGVNPKQMSAVAFGEYRPIDD
                     NNTPEGRAYNRRVEIVILRDKGVIEPTTPGIVRPLPDEEWR"
     gene            1739..2275
                     /locus_tag="PK348_05955"
     CDS             1739..2275
                     /locus_tag="PK348_05955"
                     /inference="COORDINATES: protein motif:HMM:NF015693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HXK65792.1"
                     /translation="MGDEERVNVEEIEEQEKPEEARATGAFETSRIIKILLYIAGAIL
                     AIFIIIGISYLVAKYVQEQRYQREQDIVLAPPPPPLAHFDMPSFSITTNDPEPHFAKI
                     TISLGYEENVELNAELVQRTPQMQHVVNILLRDKSYEDLNSVEDTVNLAEEIKAHINV
                     LLLKGKVKEVYFREFVVN"
     gene            2357..>3135
                     /locus_tag="PK348_05960"
     CDS             2357..>3135
                     /locus_tag="PK348_05960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65793.1"
                     /translation="MGDGSLSQDEIDALLQGADDMLSGTARVPAGEFAPAQAALSPVE
                     RESVADFLNSSMGSVMPALSGYLGKNIIIGNALVEVKSADQVRAEFPKQYVQIVVNFS
                     GGLNGKNVILLSMNDAGVISSLMMGDDSGTPPATLTEAHQSTLQEFVSQMMSTIATQL
                     SNKIGKGVNTTAPDLKVVQGNIEVPGTPDVVKVTYNLTIQNLINSKMYHLMNIELAGE
                     IARGAYVPQMQQPEYRPEPAQPRTTSQVGISPVRFPPLGEG"
BASE COUNT          973 a          533 c          704 g          925 t
ORIGIN      
        1 aggtttaatt gttggtttta ccaacataat tatggggata atttttgcca aaggtaatcc
       61 tttgctcttt tttgatatac cttcagtatt tattactata ggcggttcct tttgtgcttt
      121 gttaatatcc aatccattaa gcaaggtact gggaatatgg aaagttgcca aacatgcatt
      181 tttcccacca aagtataatc caaacgaact tatcattacc cttgtttcct tttctgaaaa
      241 agctcgccgt gagggattgc ttgcactgga agatgatctg gatgaactgg atgaccagtt
      301 tttacgcaag ggcatacagc ttgttgttga tggtactgac cctgaactgg tacgccgcat
      361 tatggaaaca gagcttgaaa acattatggc acgccatgat gaaggtaaaa agctttttga
      421 tgactgggca acatatgctc cagcctttgg tatgattggt acactgttag gacttgttct
      481 tatgctggtt aatatagaag ataaatcagc tattgggcct ggtatgtctg cagctcttat
      541 caccacattc tatggtgcta tcatggcgta tctggtactc tatccaattt catctaagtt
      601 gggttttctt aataaccagg aagtattgat gaaacttatt atgatagaag ggacattgtc
      661 aatacagtca ggtgataacc cgcgaattgt taaagataaa ttggtatcat tccttgatcc
      721 aagattgcgt gaagccatta ccagtgaggt aggtgactga tgccaaaaag aaagaaaatg
      781 gaactaccac caccaccacc atggcttacc agctggggtg atttaaatac gctgctttta
      841 acgttttttg tcatgatgtt tgatatatcc cctattattg gacaggattt taatcttgtc
      901 ctgtcttctt ttaaaggttc attggggatg atgcagggcg gatattccct ggcacgaggc
      961 aggatggaag agataggtct taatatgtta aacttacctt ccagtgagca gggaagagct
     1021 cttgcaaaga tgctgaaaag ggcagtagag gcttttaaac cagaaattca ggctaaaaag
     1081 gtaagagtgc gcgaagacga acggggcctg gttattacat tatcaagtga tgcctatttt
     1141 gaaccaggga gtgcaaaact taaggatgat attataccaa ttttaaagaa agttgccctg
     1201 attataaagt caatgcccaa ctatgtgagg atagaaggtc atactgataa cagacctgtt
     1261 cctccacgag gtcttgaaga agggtatgct actaattggg agctttcctc agcacgagcg
     1321 gttaatgtgt tgagatatct catagaggaa acaggtgtta atccaaagca gatgtcagca
     1381 gttgcctttg gtgaatacag gcccatagat gataataata cacctgaagg cagagcctat
     1441 aaccggagag ttgagatagt tatactcagg gataaaggag taattgaacc aactacaccc
     1501 gggattgtaa ggcctttgcc tgatgaagaa tggcgttaat aagaaatgta aagggagaag
     1561 tacaaaatat tgaaaaaaat tgatacattt tagtgacata atcataaatt aattgacttt
     1621 tttaattaat atagtattat gtcgataaat ttagtataac aattaaccaa caatgtttgt
     1681 ttagatttta ttgatataat actaaatgaa aatgtaaatt gttggtgagg tataacgcat
     1741 gggtgatgaa gaacgggtca atgttgaaga aatagaggag caggaaaagc cggaagaggc
     1801 cagggctaca ggtgcttttg agacttcgcg tattataaag atcctgctct atatagctgg
     1861 cgctatactg gcaatattta tcattattgg aatttcgtat cttgttgcta aatatgttca
     1921 ggagcagcgt tatcagcgag aacaggatat agttcttgct ccacctccac caccgctggc
     1981 tcattttgat atgccatcat tttcaataac aaccaatgat ccagaaccgc attttgcaaa
     2041 gattacaatt tcattaggat atgaggaaaa tgttgaatta aatgctgaac tggttcagcg
     2101 tactccccag atgcagcatg tggttaacat ccttttacgt gataaatcct atgaagatct
     2161 gaattctgtt gaggatacgg ttaatttagc tgaagagata aaagcacaca ttaatgtatt
     2221 gcttttaaaa ggcaaggtca aagaagtcta tttcagagaa tttgttgtaa attagtgtaa
     2281 tattttgtat tattaattat ggtaagtatt tttcaaagaa ttgtatatgt aagaattaaa
     2341 cacactggag gaaacaatgg gtgatggttc attgtcccag gatgaaatag atgccctgtt
     2401 gcagggtgct gatgatatgc tctcaggtac tgcaagggta ccagcagggg aatttgctcc
     2461 tgctcaggct gctttatcgc cagtagaaag agaatcagta gctgattttt tgaatagcag
     2521 catgggcagt gtaatgccag cattatcggg ctatttaggt aagaatatta ttattggcaa
     2581 tgccctggtg gaagtaaaaa gtgccgacca ggtgcgtgcc gaatttccca aacaatatgt
     2641 acagattgta gtaaattttt ctggtgggtt aaatggcaag aatgtaattt tactgtcaat
     2701 gaatgatgcc ggtgtcattt catcattaat gatgggagat gattcaggta caccacctgc
     2761 aactctaact gaggctcatc agagtacatt gcaggaattt gttagccaga tgatgtccac
     2821 tatagcaaca caattgagca ataaaattgg taaaggtgta aataccacag ctcctgattt
     2881 aaaagtagtt cagggtaata tcgaagttcc aggaactccg gatgtagtga aggtaaccta
     2941 taaccttact atccagaatt taatcaactc caaaatgtat catctgatga atatcgaact
     3001 tgcaggtgag attgcccgtg gtgcttatgt accgcaaatg cagcaaccgg aatacaggcc
     3061 tgaaccggca cagcccagaa caacttcaca ggtgggtata agcccagtac ggttcccgcc
     3121 actgggtgaa ggcat
//
LOCUS       DAOSZP010000233         3134 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_24414_length_3134_cov_3.050016, whole genome shotgun sequence.
ACCESSION   DAOSZP010000233 DAOSZP010000000
VERSION     DAOSZP010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3134
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24414_length_3134_cov_3.050016"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..171)
                     /locus_tag="PK348_05965"
     CDS             complement(<1..171)
                     /locus_tag="PK348_05965"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004429403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HXK65794.1"
                     /translation="MKPKDGYSAEELLNSTQGLSYNDFIILPGYIDFNPDDVDLETQF
                     TRNLKLKRPLVSS"
     gene            complement(307..1656)
                     /gene="radA"
                     /locus_tag="PK348_05970"
     CDS             complement(307..1656)
                     /gene="radA"
                     /locus_tag="PK348_05970"
                     /inference="COORDINATES: protein motif:HMM:TIGR00416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HXK65795.1"
                     /translation="MPKKPKTIYICNECGATFQKWLGKCPECNTWNTIVEEMQDTPVF
                     KNEIPFENSIHPLSQVLSESFQRIPTGIGEFDLVCGGGVVPGSIILLGGEPGIGKSTL
                     ALQVAQHCTTLYCSGEETLQQIHLRSRRLNIKPDSIHLTSVTDIDHIEALIRTNAPQL
                     VIIDSIQTLYSKDITSPVGSVSQIRYTASRLADIAKQTQTSILLIGHITKDGSIAGPK
                     ILEHIVDTVLYFEGDFTRDYRILRAFKNRFGSINEIALFTMTATGLTEVKDKNTLFVQ
                     SHDASFPGSVISATIEGTRTILFEAQSLVTATSFSNPRRMADGFDVNRLNLLVAVIEK
                     HAKISLSSFDIFINVAGGFTIDDTSCDLAIAAAIISSLQNIVIPHSIGIIGELSLSGQ
                     VRSASHSLRRLIEFVNSGITTILLPRNNVSEISNTGFTGTLIPLTTIAELTQILQRL"
     gene            complement(1659..2534)
                     /locus_tag="PK348_05975"
     CDS             complement(1659..2534)
                     /locus_tag="PK348_05975"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HXK65796.1"
                     /translation="MRLSNEAKVGLLVMTSFTLFIVLVGLLTKFNISQKGYRLHVYFG
                     FLNDLRVGAPVKIGGGIKIGYVDEIRQTGEKTDVILWIDKGYRLSKAATFSIFTSGMI
                     GEKYINVVIPPMSEDVGFLNDGDIKYGIDPASFDQMMQTFQSFMQDKSGAQILAEIFQ
                     NSNKFVANLNKMVDENRYDVRQSVGTARAAIVTFSQQLQQFMNQLNVISKNMAYISEK
                     NREDITITLQNLSELTSSMNKIAYRLEKGRGTMGKLLYDEEIYTNIRDASISAKELFS
                     RLEKDPSLLLFKQKK"
     gene            2710..>3134
                     /locus_tag="PK348_05980"
     CDS             2710..>3134
                     /locus_tag="PK348_05980"
                     /inference="COORDINATES: protein motif:HMM:NF014857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HXK65797.1"
                     /translation="MRYLVVGSGGREHAIAWRLIHDGSASEVYVAPGNGGIDERFRVQ
                     VAVNDFESLYTICKQKNIDIVIIGPEAPLVDGVVDFLQNRGIPVFGPTAQAAQLEGSK
                     LFAKYIMQKYNVPTAHFREFKGKESLIGYIESSAVFPVV"
BASE COUNT          942 a          559 c          634 g          999 t
ORIGIN      
        1 gcttgatacc agtggccgtt taagtttaag attgcgggta aattgtgttt ctaaatcaac
       61 atcgtcaggg ttaaagtcaa tataacccgg taatatgata aaatcgttgt aggataaccc
      121 ctgagtggaa tttaaaagct cttcagctga atagccgtct ttaggtttca tgtagtacct
      181 ctatgagtca atgaataata ttttctatag tgttgtggtg ttatataaga tatagcatgg
      241 agcgcactat atttcaatta tttgcttaag tattacgtac aatgtttcaa taatcaactg
      301 aaaaaattaa aggcgctgta aaatttgtgt aagctctgcg atagtagtta aaggtatgag
      361 tgttcctgta aagcctgtat ttgaaatctc agatacattg ttgcgaggta acagaatggt
      421 ggttattcct gaatttacaa attctataag cctgcgtaat gaatgacttg cagaacgaac
      481 ctgtccagaa agtgataatt cgccaatgat gccgattgaa tgcggaataa ctatattttg
      541 taaacttgat ataatagcag cggcaattgc aaggtcacac gatgtatcat caatggtaaa
      601 tccgcctgca acattaataa atatatcaaa tgagcttaat gaaatctttg catgcttttc
      661 aatgaccgct acaagcaaat taagcctgtt aacatcaaaa ccatctgcca ttcggcgagg
      721 gtttgaaaag cttgttgcag taacaagcga ttgtgcttca aagagaatgg ttctggtccc
      781 ctctatagta gcactgataa cgcttcccgg aaagcttgca tcatgcgatt gaacaaataa
      841 tgtgttcttg tcctttactt ctgttaatcc tgtggctgtc atggtaaata atgctatctc
      901 attaattgag ccaaaacggt ttttaaaggc gcgtaatatg cgatagtcac gcgtaaaatc
      961 cccctcaaag tagagtactg tgtcaacaat atgttccagt atttttggac ctgcaatgga
     1021 gccatccttg gtgatatgcc cgattagcag tatagaggtt tgcgtttgtt ttgcaatatc
     1081 agcaagccgt gaagcagtat agcgaatctg gcttacggaa ccaacaggtg aagttatatc
     1141 ttttgaatag agtgtctgga tagagtctat tattacaagc tgtggtgcgt tagtgcgtat
     1201 gagtgcttcg atatggtcaa tatcagtaac tgaggtaagg tggattgaat caggttttat
     1261 attaagccgg cgtgatcgca aatgtatttg ttgcagagtt tcttctccag agcagtacag
     1321 agttgtgcaa tgctgagcta cctgtaatgc taatgtggat ttgcctatac ctggttcgcc
     1381 tcccagaagg ataatggagc cgggaactac tcctccacca cacaccagat caaattcgcc
     1441 gattcctgtt ggtatacgtt gaaatgattc tgatagtacc tgcgaaaggg gatggatgga
     1501 attttcaaag ggtatttcat ttttaaaaac aggagtatcc tgcatttctt caacgatagt
     1561 attccaggta ttgcattccg ggcatttccc taaccatttc tggaatgttg ctccgcattc
     1621 attgcagata tatattgttt ttggtttttt aggcattgtt attttttctg cttaaagagt
     1681 agtaatgatg gatctttttc aagccttgaa aaaagctctt ttgctgaaat ggatgcatca
     1741 cgtatgttgg tataaatttc ttcatcatag aggagctttc ccattgttcc acgacccttt
     1801 tcaagacggt atgcaatttt attcatgctg gatgtgagtt cagaaagatt ttgtaatgta
     1861 atggtaatat cttcacggtt tttttcagag atatatgcca tatttttgga aataacattc
     1921 aactgattca tgaattgttg caattgctgg gaaaaagtta cgatagccgc acgtgcggta
     1981 ccaactgact gtcgaacatc ataacggttt tcatcaacca tcttatttaa gtttgccaca
     2041 aatttatttg aattctgaaa tatctctgct aggatctggg ctccactttt atcctgcata
     2101 aagctctgga atgtctgcat catctggtca aagcttgcgg ggtcaatacc atacttgata
     2161 tccccgtcgt taagaaaacc gacatcctca ctcattggag gaatgacgac attaatatat
     2221 ttttcaccaa tcatgcctga tgtaaagatt gaaaatgttg ctgcttttga taaccggtat
     2281 cccttatcta tccacagaat aacgtcagtt ttttcacctg tctggcggat ctcatctaca
     2341 tatccaatct tgattccacc accaatttta acaggggcac caacgcgaag gtcgtttaaa
     2401 aatccaaaat atacatgtaa gcggtatcct ttttgtgaaa tgttaaattt tgttaacagc
     2461 ccaaccagta caataaaaag cgtaaagctt gtcataacca gaagcccaac ctttgcttca
     2521 ttactcaggc gcatagcaaa cctcctcata taagggtgtt gttatctcat atactgtaca
     2581 taacgtaagt tcaagaatta ttcttataat atataaaaaa tattgtacgc atgaaagatg
     2641 tatacataaa atgattgaaa aattatatct cttgtttaca acaataccag catatgacag
     2701 gaggaatcaa tgagatattt agtagtaggg tctggtgggc gtgaacatgc cattgcatgg
     2761 agactcattc atgatggaag tgccagtgaa gtatatgttg caccaggtaa tggtggtatt
     2821 gatgaaagat tccgggttca ggttgccgtg aatgattttg aatcattata cactatctgc
     2881 aaacaaaaaa atattgatat tgttataatt gggcctgaag ctcctcttgt tgatggagta
     2941 gttgattttt tacaaaatag aggtatacca gtatttggtc caacagctca ggctgcccaa
     3001 ctagaaggga gcaagctttt tgcaaagtat atcatgcaaa aatataatgt gccgacagct
     3061 catttcagag aatttaaagg taaagaatca ctcataggct atattgaatc ttcagcagta
     3121 tttccggtag taat
//
LOCUS       DAOSZP010000234         3127 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_24476_length_3127_cov_3.310221, whole genome shotgun sequence.
ACCESSION   DAOSZP010000234 DAOSZP010000000
VERSION     DAOSZP010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3127
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24476_length_3127_cov_3.310221"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..551
                     /locus_tag="PK348_05985"
     CDS             <1..551
                     /locus_tag="PK348_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008938143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HXK65798.1"
                     /translation="NDYERYQTVFARKYGAAAAPTAGLHFTDDLLKQIQDKGATISFL
                     TLYVSWGTFQPVRVDDIRLHTMHTEEYYLPEKTVESIYNAKAKGGRVIAVGTTSLRVL
                     ESVYHNGEYKHGYGATNIFIYPPYTVKSVDALITNFHTPGSTLLMLVAAFAGYDCIMQ
                     TYREAVKERYRFFSYGDAMFIT"
     gene            785..1333
                     /locus_tag="PK348_05990"
     CDS             785..1333
                     /locus_tag="PK348_05990"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HXK65799.1"
                     /translation="MLLKGCLHTHTTCSDGTLTPQQVAVRYEEKGYDFIAFTDHDYLL
                     KPGYDEIYNNVKSNMIIFPGIEMTVFLKGYIHVNRIFGDNQLLHIFNHLPEYDLDIEQ
                     IMDRLYNLQKMFAIDAMEITSKGFKVKRFNEMTFPHPSIVSDDAHTAIGIGRAWIELD
                     AKRNKDSILKAIKHGDFWNCYC"
     gene            1396..2541
                     /locus_tag="PK348_05995"
     CDS             1396..2541
                     /locus_tag="PK348_05995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HXK65800.1"
                     /translation="MQKRVAICAVAQIKNDPNLWYARFQNMLLECFEGIMSQTKVSFD
                     MEKGIRTVITCSDDVWDARTISNNGMTDVVGAHFRGEEKMAQESLNGLGYAMASILSG
                     HDDVVLYMGHMKESQPESRTMCTNLAFDPFYCRPLGMDFVNVNAMQARAYMEKSGVNE
                     KHLAKIVVRSRQKAKQNPFARENEPIKEDDVMKSPLLADPLRQLHYYPTTDWAFGMLL
                     CSEERVKEFTDKPVWITGYASCMDSYFLGDKDLTSNFSLRNATERALKKAGIKDVKKD
                     IQLFELCDHAAYQLPMWAEGIGLAKEGKGGKWIDDGGMDEFNVNMSGGMLNGNPLLLG
                     GAARAIECFYQLRGEAGKRQVKGAKRALAQSTYGAAGQHQAVVIFEV"
     gene            2555..>3127
                     /locus_tag="PK348_06000"
     CDS             2555..>3127
                     /locus_tag="PK348_06000"
                     /inference="COORDINATES: protein motif:HMM:NF012336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HXK65801.1"
                     /translation="MAQKKDRNVAIIGIGQTKHSSHREDVNQPEMIHEAVSAALKDAN
                     ISIKDVDCIVHGNMELFEMIHQPDLWHVLGTGAYGKDSIRITTGGTVGTTLACASDNL
                     VASGMYDIVMAIGFEKLQEGHTTGGITNMADPLWFRNLQTGALTGSTAYDVIQEFGED
                     RAKRAAMMYRIIMDKHACLNPNAHRAFGLDF"
BASE COUNT          985 a          497 c          721 g          924 t
ORIGIN      
        1 aaaatgatta tgaacgctac caaacagtat ttgccaggaa gtacggtgca gcagcagcgc
       61 ccacagcggg attgcatttc actgatgatt tgctgaaaca gatacaggat aaaggtgcta
      121 ctatttcatt tttaaccctg tatgtatcat ggggcacatt tcaacctgtt agagtggatg
      181 atattaggct acatacaatg catacagaag aatactattt gccagaaaaa accgtggaat
      241 ctatctataa tgcaaaggca aaaggaggaa gggtgatagc tgttggcacc acatcactgc
      301 gtgtacttga atcagtttat cataatggtg agtataagca tggatatgga gctaccaaca
      361 tttttattta tccaccctat actgtcaaat ctgttgatgc gctgattacc aattttcaca
      421 ctcccggatc aacattgctt atgcttgttg ctgcgtttgc tggatatgat tgtattatgc
      481 aaacatacag ggaagcagtt aaggaacgat acagattttt ttcatacggt gatgcaatgt
      541 ttattacatg atgggacgag ttgtacatta ttatttattt gtacataagt tgctgttaaa
      601 aaaataatca tccaaacaga aaaattttta aaaaattatt gacatattgt attatattaa
      661 ttaatttgca aattgattgg atgtccaata aattatatgt tgcttcaaat ttttattttt
      721 ttatcaaaat attggttgga cattcattca attgtttaaa aaattaatta aattgtataa
      781 tacaatgctt ttgaaagggt gcctacatac gcataccaca tgttctgatg ggacgcttac
      841 accccagcag gttgctgtgc gttatgaaga aaaaggatac gattttatag ctttcaccga
      901 tcacgattac ctgttaaaac ctggctatga tgaaatctat aataatgtta aatcaaatat
      961 gattattttt ccaggtattg aaatgactgt gttcttgaaa ggatatatcc acgtaaacag
     1021 gatttttggt gataatcagt tgcttcacat atttaatcat ctgccagagt atgaccttga
     1081 tatagaacag attatggata ggctttataa tcttcagaaa atgtttgcca ttgatgctat
     1141 ggaaataact tccaaaggat ttaaggttaa acggttcaac gaaatgacat ttccgcatcc
     1201 ttcaatagtc tctgatgatg ctcacacagc tataggtatt ggtagggcat ggatagaact
     1261 ggatgcaaaa aggaataagg attcaatatt aaaagccatc aaacatggtg atttctggaa
     1321 ctgctattgt taggcatggg tataatgtga atttaccata taatatttaa tatttaaaaa
     1381 ggaggttcat agggtatgca aaaacgagtt gctatctgtg ctgttgctca gattaagaat
     1441 gaccccaatt tgtggtatgc gcgtttccag aatatgcttc tggaatgttt tgaaggcata
     1501 atgtcacaaa caaaggtgag ctttgatatg gaaaagggca tacgtactgt tattacctgt
     1561 tctgatgacg tgtgggatgc gcgtacaatt tcaaataatg gcatgactga tgttgttggt
     1621 gctcatttca ggggtgaaga gaagatggcg caggaaagcc ttaatggttt ggggtatgct
     1681 atggcaagca tactctctgg gcatgatgat gtggttcttt atatgggaca tatgaaagaa
     1741 tcgcagccag aaagccgtac tatgtgtaca aatcttgcat ttgacccatt ctattgcagg
     1801 ccactgggga tggattttgt taatgtcaat gcaatgcagg cacgggcata tatggaaaag
     1861 tccggggtaa atgaaaaaca tctggcaaag attgtggttc gttcaaggca aaaagctaag
     1921 caaaacccct ttgcacgtga aaatgaacca ataaaagaag atgatgttat gaagtcgcct
     1981 ctattagctg atccactgcg gcaactgcat tattatccaa caaccgattg ggcttttggc
     2041 atgttgcttt gtagtgaaga acgtgtaaaa gaatttaccg ataagccagt gtggataacc
     2101 ggctatgcaa gctgtatgga tagttatttc cttggtgata aggatttaac atcaaatttt
     2161 tcattacgaa atgcaactga aagggcattg aagaaagcag ggatcaaaga tgttaaaaaa
     2221 gacattcaac tttttgaatt gtgtgaccat gctgcatacc agttgccaat gtgggctgaa
     2281 ggcattggtc tggctaaaga aggcaaaggc gggaagtgga ttgatgatgg tggcatggat
     2341 gaatttaatg tcaatatgtc tggaggcatg ttaaatggca atccattgct tttaggtggt
     2401 gctgccagag ctatcgaatg cttttatcag ctaagaggtg aagctggtaa acgtcaggtt
     2461 aaaggtgcaa aacgtgcatt ggcacagagc acctatggtg cggccggtca acaccaggct
     2521 gttgtgattt ttgaagtgta aaggaggggt gaccatggca caaaaaaagg atagaaatgt
     2581 tgcaattatt ggtataggac aaaccaagca ttcaagccat agagaagatg tcaatcagcc
     2641 tgagatgatc catgaagcag tatctgctgc gcttaaagat gctaatataa gcatcaagga
     2701 tgtagattgt atagttcatg gtaatatgga actatttgag atgattcacc agcctgattt
     2761 atggcatgtg cttggaacag gagcgtatgg gaaggacagc atcagaataa ctactggtgg
     2821 aacggtgggt acaacgcttg catgcgccag tgacaatctg gttgcgtcag gaatgtatga
     2881 catagtcatg gcgattggat ttgagaaact gcaggaaggt catacaacgg gtggtattac
     2941 caatatggct gatcccctat ggttccgaaa tttgcagaca ggtgcattaa caggatccac
     3001 tgcatatgat gtcattcagg agtttggtga agatagagca aaacgtgcag caatgatgta
     3061 tcgtattatc atggataagc atgcatgttt aaatcccaat gcacacaggg catttggcct
     3121 ggatttt
//
LOCUS       DAOSZP010000235         3107 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_24672_length_3107_cov_3.265727, whole genome shotgun sequence.
ACCESSION   DAOSZP010000235 DAOSZP010000000
VERSION     DAOSZP010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3107
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24672_length_3107_cov_3.265727"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..411)
                     /locus_tag="PK348_06005"
     CDS             complement(<1..411)
                     /locus_tag="PK348_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015949184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HXK65802.1"
                     /translation="MRECVFIDGVRTPNGRAHREKGWFRHLRPDELLTPVYNALFERN
                     SKVAPDMVDAVFVGCANPSGMQNDIGRLAWLASGLPDSVPTNTLTNQCPSGMSATMHA
                     ARAILTGEADIIIAAGVEDMEKVPMGANMDFPPRL"
     gene            complement(437..2314)
                     /locus_tag="PK348_06010"
     CDS             complement(437..2314)
                     /locus_tag="PK348_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015949185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HXK65803.1"
                     /translation="METLPWYKEYKVLGIPKTFKPYPDKPVYDILEIAAKKYKKNGLL
                     QKDYFISYPQLLDYVNRLASRLQSFGLIKNDIIATILPTSTEFIIADYAISKAGLIHL
                     PCSSLEPAEALEHKFKEGKPKAIICTSNDIKIVTSLIKKTSVRAIIIANTRTSTSFPT
                     IDTDKIKISGSTVYELYSLIQNSSGNVNPVPINVETDIETLIFTGGTTGIPKGCMLTH
                     RNIYANCIQNLHALGAGGLTFKGAISVILGLPFFHTYGHCLMHTMMLFGFNIIIINDP
                     RDTKSMVENIKKHYPVMQIGVPAQFMKLAKEDLSGIGIIGLSGSAPLSESTQEEFEKR
                     SNSGIMEGYGLSEMSPVTHLNPSVIYRILGGRFGVRLVSLLSSIPGNSYLTNQLFRLI
                     GTRNFGWIFTKIIAYRMKKTSQKPGKKSVEKRRTIGVPFPDTEVRFLDFDSGNILTID
                     DMLAGKRAEMLLKGPQRMLGYWPKPDSGCDAEGFIHTGDIVQIDSNGYFYIVDRSKDM
                     INVSGFKVYSKEIDDIAGEHPHIEAAATVGIPNPEKEGSEVVVLFVQLYPQYKNKTKE
                     QDILKYLQSKVAKYAVPKFIKIVDELPRTAVEKVDKKILRQMAIDQFLQSKKKVKIKT
                     K"
     gene            complement(2389..2946)
                     /locus_tag="PK348_06015"
     CDS             complement(2389..2946)
                     /locus_tag="PK348_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015949186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HXK65804.1"
                     /translation="MVIAPNTSIDQFLTEIMPKVATEMLQNSNAPKELGGTEITMVVE
                     VNGKSYGFKVKDGKDIETCGDIANAMLRLKISEADLKKMIETNNLDMLLGMQKDLTRA
                     RYNALSSLKGSFIAKLKNDDGSEFTIEAILNGATSPQSIFRMTTKDSAALMKKETNPV
                     NLFMSGAMKIEGDMSFAMATQPLFT"
BASE COUNT          916 a          559 c          541 g         1091 t
ORIGIN      
        1 caatcgtgga gggaaatcca tattggcacc cattggtact ttttccatat cttctacacc
       61 agcagcaatg ataatatctg cttcgccagt caaaattgct cgtgcagcat gcattgtggc
      121 ggacatgcct gatgggcact gattggtcaa agtattggtg ggaactgaat ctggtaatcc
      181 agatgctaac catgctaacc tgccaatatc gttttgcatg cctgatggat tagcgcaacc
      241 aacaaaaaca gcatcaacca tatctggagc cactttgctg tttcgctcaa atagagcatt
      301 atacactggt gttaaaagct catcaggcct taaatgtctg aaccagcctt tttcacgatg
      361 agctctgccg tttggtgttc tcacaccatc aatgaataca cattctctca ttggtacctc
      421 tcttgttctc taattattat tttgttttga ttttcacttt cttttttgat tgtagaaact
      481 ggtcaatagc catttgacgt aatattttct tatcaacttt ttcaactgct gttcggggaa
      541 gttcatcaac aatttttatg aacttgggta ctgcatattt tgcaaccttt gattgaaggt
      601 attttaatat gtcctgttct tttgttttat ttttgtattg tgggtacagt tgaacaaaaa
      661 gaacaactac ttcgctgcct tctttttcag gatttggtat tccaacagtt gcagctgctt
      721 caatatgtgg atgttctcca gcaatatcat caatttcttt tgaatatact ttgaaacctg
      781 agacattaat catatctttg gaacgatcaa ctatatagaa ataaccattt gagtctatct
      841 gtactatgtc accagtatga atgaaacctt cagcatcaca tccactatca ggttttggcc
      901 agtatcctaa catacgctgt ggccccttta aaagcatctc tgctcttttc ccagccagca
      961 tatcatcaat tgtcaggata ttaccagagt caaaatcaag aaaacgtact tcagtgtcag
     1021 gaaatggaac accaattgtt cttctttttt caacagattt ctttccaggc ttttgactgg
     1081 tttttttcat acgatatgca ataattttag taaaaatcca gccaaagttc cttgtcccta
     1141 tcaaacgaaa gagctgattg gttaagtaac tatttccagg aattgatgat aagagagaaa
     1201 ctaatcggac gccaaaacgt ccacctaaaa tacgatatat tactgaaggg ttaagatgtg
     1261 taaccgggga catctctgaa agtccatagc cttccattat gccgctattt gaacgttttt
     1321 caaattcttc ctgcgttgat tcagataaag gtgctgatcc tgaaagacca ataatgccta
     1381 taccactgag atcttcttta gctaatttca tgaattgagc tggtacacct atctgcataa
     1441 cgggatagtg cttcttgatg ttttcaacca ttgatttagt atcacgtgga tcgttgataa
     1501 tgatgatatt aaaaccaaaa agcatcattg tatgcatgag acaatggcca taggtatgga
     1561 aaaatggtaa tcccaatata acagatattg cccctttgaa tgtcaatcct cctgctccta
     1621 atgcatggag attctgtatg cagttagcat atatgtttct atgtgtgagc atacagcctt
     1681 ttggaatacc tgtggtcccc ccagtaaata tcaatgtttc aatatcagtt tcaacattga
     1741 tgggaactgg gttaacattt cctgaagaat tttggattaa tgagtataac tcatatactg
     1801 ttgaacctga tattttaatt ttatctgtat cgatagttgg gaaagaagtg ctggtacgtg
     1861 tgttagcaat aatgatggct ctgacactgg tctttttaat caagctggtt actattttaa
     1921 tatcattgct ggtacaaatt atagctttag gtttaccttc tttaaattta tgttcaaggg
     1981 cttcagcagg ttcaagtgaa ctacatggca gatggatgag ccctgctttt gaaattgcgt
     2041 aatcagcaat tataaattct gtagaagttg gtaaaatcgt tgcaataata tcatttttaa
     2101 ttaatccaaa agactgaaga cgtgaagcta atctgtttac atagtctaga agttgcggat
     2161 atgatataaa ataatccttt tgtaaaaggc catttttttt gtattttttc gcagctattt
     2221 caagaatatc atatactggt ttatcaggat aaggtttaaa tgtttttggt atacctaaaa
     2281 ctttatattc tttgtaccag ggtaaagttt ccattgttaa cctccaggtg atcgataaaa
     2341 aaacatctca accacataaa aatgtggttg agatgtaaag catatctatc aagtgaatag
     2401 gggttgggtt gccattgcaa aactcatgtc accttcgatt ttcattgcac ctgacatgaa
     2461 aagattaaca ggatttgttt cctttttcat taaagcagct gaatcttttg ttgtcatgcg
     2521 gaaaatcgat tgtggtgatg ttgcaccgtt aagtattgct tcaatggtaa attcagaacc
     2581 atcatcattt tttaatttag caatgaaact acctttcaaa gaagagagag cgttatatcg
     2641 agctcttgtc agatcctttt gcatgcctaa caacatatcg agattattgg tttcaatcat
     2701 tttctttaaa tcagcttcgg atatttttaa tctcagcatg gcattggcga tatcaccaca
     2761 tgtttcaata tctttaccgt ctttaacttt gaatccatag gatttaccat taacttcaac
     2821 aaccatggtg atttcagtgc caccaagttc ttttggtgca ttggaatttt gaagcatttc
     2881 tgttgctact tttggcataa tttctgtaag aaactgatca attgaagtat ttggtgcaat
     2941 aaccatagtg gtcctcctta aaaataatat tatgtaaaat atttttgcaa atataaaaat
     3001 tttgcttgta atactgacat gtcagtatta ccatgttttg tcaagattaa tttatgaaaa
     3061 aaattatttt ttttaaattt cttattaaga atttaattgt tttttat
//
LOCUS       DAOSZP010000236         3104 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_24707_length_3104_cov_2.953099, whole genome shotgun sequence.
ACCESSION   DAOSZP010000236 DAOSZP010000000
VERSION     DAOSZP010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3104
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24707_length_3104_cov_2.953099"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..712
                     /locus_tag="PK348_06020"
     CDS             <1..712
                     /locus_tag="PK348_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012610207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HXK65805.1"
                     /translation="QQFEEAIAANPDGIAGFIATPYHHPTFADSEFPAEGYWQRIREL
                     CTKHGIVLIIDDVRCGFRLDMRGSGAYFGFKPDLACYCKAIANGYPISALVGIDALRT
                     VAAKVFYTGSYWYQAVPMAAALATLKELKRINGPVLMNKIGKKLFDGMVKICGQYGYD
                     LKVTGAYSMPYVRITNDPSNMLHQEWCAECTARGAYFTSHHNWFVSAAHTEKDIQKTW
                     NIVDDVCKLLKKKYGEKY"
     gene            806..1648
                     /locus_tag="PK348_06025"
     CDS             806..1648
                     /locus_tag="PK348_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012174556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HXK65806.1"
                     /translation="MPLSQQELKQLNEKACQIRKDTIDTVYWAGGGHVGGSLSFTEIM
                     VILYYKYLNIDPKNPQWPDRDRVVLSKGHAGVGYAPILADKGYFPKELLKEYNHTGSI
                     LGMHLDGNKVPGVDVSTGSLGHGLPIAVGLALGARLQNKSWLTYCIMGDGECDEGSVW
                     EAAMAAAHYKVTNLVTIVDRNRMMIDGPTEEVMRLEPFADKWKAFGWIVKEVDGHDFN
                     QLADAIEYAQKEDKAPVVIIAHTIKGKCVDFMEDKYEWHYGGLDSEKVELAKACIDKH
                     YCEM"
     gene            1664..2677
                     /locus_tag="PK348_06030"
     CDS             1664..2677
                     /locus_tag="PK348_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012174555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HXK65807.1"
                     /translation="MAADGLTYTIVDTANMSTAEIYGKVLCDLAEKHNDIVGLSADLA
                     KSTKIGQFAAKFPDRFFNAGIAEQNMFGIAAGLEKSGLTPFVSTFAVFASLRAAEQVR
                     TDICYPKLNVKIIATHAGTSFGQAGTTHHCTEDLAVMRSFANMVVIAPADGIETANAV
                     IAAYEYKGPVYIRINRGFDPFLYEKSDYGFQIGKAVQLKDGTDITIIACGAAAYNAYH
                     AAGILEKEDGLSVRVLNMHTIKPIDEEAILKAVMETRRIVTFEDHNIYGGLGSAVAEV
                     IVKSGKACAFESVGIPDEYAIIGLPEDLQNYYGFDQNKIVEKVRQIMGKDFEQTEDWE
                     DEV"
     gene            2679..>3104
                     /locus_tag="PK348_06035"
     CDS             2679..>3104
                     /locus_tag="PK348_06035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65808.1"
                     /translation="MATQNELFASTIFLKAALPLTKVILEYRPKMAAKYKGLNGVVQF
                     IAKDEAGDIGAYLQFTDGVLDVKYGIHEKPDIVFTFKDVKTMNAFFAGKPAVPSIKGL
                     TKFKLLMKAVNLLLALKIIGRKTPPKKAEDRELMVRLMLY"
BASE COUNT          945 a          506 c          764 g          889 t
ORIGIN      
        1 tcaacagttt gaagaggcaa ttgctgctaa tccggatggt attgcgggat ttatagctac
       61 gccttaccat catccaacat ttgccgatag tgagttccct gcagaaggtt actggcagcg
      121 tattcgtgag ttgtgtacca aacatggcat agtgcttatt attgatgatg tccgttgtgg
      181 gttcaggctt gatatgcgcg gatcaggtgc atattttggg tttaaacctg atttagcctg
      241 ttactgtaaa gctattgcca atgggtatcc catttcagca ctagttggta ttgatgcact
      301 acgaactgtt gctgcaaaag ttttttatac aggaagttac tggtatcagg ctgtacctat
      361 ggctgcagca ctggctacat tgaaagaatt aaaaaggatt aatggtcctg ttcttatgaa
      421 caagattggc aaaaaacttt ttgatggtat ggttaaaatt tgtggacagt atgggtatga
      481 tttaaaagtt accggtgcat attctatgcc gtatgttcgc attaccaatg atccaagcaa
      541 tatgctgcat caggaatggt gtgctgagtg tacagctcgt ggtgcttatt ttacttcgca
      601 tcataactgg tttgtatcag cagctcatac agagaaggat attcagaaga catggaatat
      661 cgttgatgat gtgtgcaaat tgttgaagaa gaaatatggt gaaaaatatt aatgcttgca
      721 attgtaagat acaaggggtt atcatagcag taactatcgc ccataagaat taaataaaaa
      781 attacaaagg acaaggagga tatatatgcc gttaagccag caggaattaa aacaactgaa
      841 cgaaaaagca tgccagataa gaaaggatac catagatact gtatactggg ctggtggtgg
      901 acatgtgggt ggctcattaa gttttactga aatcatggtc attctttatt ataagtattt
      961 aaatattgat cccaaaaatc cacaatggcc cgatagggac cgggtggtac tgagcaaagg
     1021 gcatgctggt gttggatatg ctccaatcct tgccgataag ggatatttcc ctaaagagtt
     1081 gttgaaagag tacaaccaca ccggttcaat actggggatg cacctggatg gtaacaaggt
     1141 accgggtgtt gatgtgtcaa ctggttcgct gggacatggt ttgcccatag ccgtagggtt
     1201 ggcattaggt gcacgcttgc agaataaatc atggctaacg tattgtataa tgggagacgg
     1261 tgagtgtgat gagggctcag tgtgggaagc tgcaatggct gccgcccact ataaagttac
     1321 taatttggta actatcgtag accgtaacag gatgatgata gatggtccaa cggaagaggt
     1381 tatgcgcctt gaaccatttg ctgataaatg gaaagctttt ggctggatag tgaaagaagt
     1441 tgatggacat gattttaatc aattggctga tgcaattgaa tatgcacaaa aagaagataa
     1501 agcaccggtt gttataattg cacatacgat taagggcaag tgtgtggact ttatggaaga
     1561 taaatatgaa tggcactatg gcgggctgga cagtgaaaag gtagaacttg ccaaagcttg
     1621 tatagacaaa cattattgtg aaatgtaaat aaggaggaaa acaatggctg ctgatggatt
     1681 aacatatact atcgttgaca ctgccaatat gtcaaccgct gaaatttatg gtaaggtatt
     1741 atgtgatctg gctgaaaaac ataatgatat tgtgggactt tcagctgatt tagctaagtc
     1801 aacaaagatt ggtcagtttg cggcaaaatt tccagacagg ttttttaatg caggaattgc
     1861 tgaacagaat atgtttggta ttgctgcagg gcttgaaaaa tcggggctca ctccgtttgt
     1921 ttccacattt gcggtatttg catccctgcg tgcggcggag caggtgcgta ctgatatttg
     1981 ttatccaaag cttaatgtaa aaattattgc aactcatgcc gggacttcat ttggacaggc
     2041 aggtaccacg catcactgta ccgaggatct ggctgttatg cgttcatttg ccaatatggt
     2101 tgtgatagct cctgcagatg gtattgaaac tgcaaatgct gtgatagcag cgtatgagta
     2161 caaaggcccg gtatatatca ggattaaccg tggttttgac cctttcttat atgaaaaatc
     2221 tgattacgga tttcagatag gcaaagcagt acagcttaaa gatggcactg atatcaccat
     2281 tatagcctgc ggtgctgctg cgtataatgc ttaccatgct gctggtatac ttgaaaaaga
     2341 agatggactg agtgtgcggg tactcaacat gcatacaatt aaacccattg atgaagaggc
     2401 catccttaaa gctgttatgg aaacgcgccg catagtgaca tttgaagatc ataacattta
     2461 tggtggactt ggtagtgcag ttgctgaggt aatagtaaaa agcggaaaag cctgtgcctt
     2521 tgaatcagtt ggtattcccg atgaatatgc catcataggg ttacctgagg atttacagaa
     2581 ttactacgga tttgatcaga ataagattgt tgaaaaagta cggcagataa tgggcaaaga
     2641 ttttgaacaa actgaagact gggaagacga ggtgtaatat ggcaacccag aacgaattgt
     2701 ttgcatcaac aattttttta aaagctgcat tgccgcttac taaagtaata ctggaataca
     2761 ggccaaaaat ggctgcaaaa tataaagggc tcaacggtgt ggtgcagttt attgcaaagg
     2821 atgaggctgg cgatattggt gcatatctgc agtttactga tggtgtactg gatgtaaagt
     2881 atggcattca tgaaaaaccg gatatagtct tcacgtttaa ggatgtaaag actatgaatg
     2941 cattttttgc cggtaagcca gcggtgccat ctataaaagg tttaactaaa tttaaattgt
     3001 taatgaaagc agttaattta ttactggctc ttaagatcat tggcaggaaa actcccccaa
     3061 aaaaggcaga agaccgtgag cttatggtaa ggcttatgct ctat
//
LOCUS       DAOSZP010000237         3096 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_24785_length_3096_cov_5.563959, whole genome shotgun sequence.
ACCESSION   DAOSZP010000237 DAOSZP010000000
VERSION     DAOSZP010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3096
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24785_length_3096_cov_5.563959"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="PK348_06040"
     CDS             complement(<1..194)
                     /locus_tag="PK348_06040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65809.1"
                     /translation="MAEAQNNIPILNRKEWQTMMPAITATAAGSFVAADQSGLSRYAL
                     YMLSGTVHYLYDHLNDDYLP"
     gene            complement(198..848)
                     /locus_tag="PK348_06045"
     CDS             complement(198..848)
                     /locus_tag="PK348_06045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65810.1"
                     /translation="MADNVLINAVTTPGGATIATDEVNGVQHELVKVEFGVDGVATMV
                     SASDPLPVTGTVEVSNFPVTQPVSGTVAVSSLPTGLATSANQQTDALTDAELRAAAVP
                     VSLSVVPLATGAATATKQDEQTAQLATLNSLIETLRSLVAVLSPLGGAMNSGAPGLRV
                     TPNAATLPISGSVTATVASTVVSSLTNFGTSIPASEMANAMNNLVATMANINNANG"
     gene            complement(850..2214)
                     /locus_tag="PK348_06050"
     CDS             complement(850..2214)
                     /locus_tag="PK348_06050"
                     /inference="COORDINATES: protein motif:HMM:NF016148.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor head protein"
                     /protein_id="HXK65811.1"
                     /translation="MNLQERKKYSRLVELVNRRFESRFYPKLQKAIKQDISSLIEYLK
                     INGIDSAPKYFDDNIASKSLTEIVQDLYSNVGVYHANRVYRDLRKEEKGFGFNPDFIN
                     FVTNYFRKHLVEKITFGAVKTMRDFFLPLISKAVTDGATFEEIAQAIRDKGFEKRQAA
                     RIVRTEVNSATNVAAVAAAEKFEYKTMKEWISGHDNRVRGRNPEDHANHWGLDGVVVD
                     YNQPFVDPRNGVRLMQPGDPNAQGMRRDKAATVINCRCTVALVAKRDANDRLIPKTGL
                     IKSVEPEETKELKPDYSEIIEPIDYLKDEIRDIGRDVRGIKETDLSPVLERFEGLATK
                     EDLDEIKLFGELISGEGERQVEVVTKVRDELERLIEEKLNGDLSRIDELIDLINSKDY
                     KPEIVLNSSSKSEIDTFRAEMMAMINLRFDELLKEVMKKRKYQFTVVKDSNDLIISAT
                     AQQV"
     gene            complement(2328..>3096)
                     /locus_tag="PK348_06055"
     CDS             complement(2328..>3096)
                     /locus_tag="PK348_06055"
                     /inference="COORDINATES: protein motif:HMM:NF016732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HXK65812.1"
                     /translation="NPEWSTNGAQLYGLSPLKAAVKRYTRNNEAMTAASVAFKNQGVK
                     GFISPDISPQDVDMGNIAIMGEQAAKVKKLLTSPEYSGADSYGKMVATAYRMNWTDIG
                     LSPVDMQIIESEKWDAVMLCNIYGVPPELLGLTQKTYNNVKEAEKALTTRCAFPLLTT
                     FRDAFNHKLNNDWGFKGKNIYVDYDFTIFSELDENMGEKVDWVNKLWMLSPNEQLNIL
                     GLQKNDNPLFDEPWIDGTKQPMSEVQANEIDKMIGDE"
BASE COUNT          772 a          803 c          477 g         1044 t
ORIGIN      
        1 attggtaaat aatcatcgtt taaatggtcg tacaaataat gaaccgtacc agatagcata
       61 tataaagcat atctggataa ccctgattgg tctgccgcta caaatgaacc tgccgccgtt
      121 gcggtaatcg ctggcatcat tgtttgccat tctttacgat ttaaaatcgg gatattattc
      181 tgcgcttctg ccataaatta tccgtttgcg ttattaatat ttgccattgt tgctactaag
      241 ttgttcatag catttgccat ttcagatgcc ggaatacttg ttccgaaatt cgttaatgaa
      301 cttactactg tacttgccac cgttgccgtt acacttcctg aaattggtaa tgtagctgcg
      361 tttggtgtaa ctctcaaccc cggtgctccg ctgttcatag ccccacctaa aggactaagt
      421 acagcaacca aacttcttaa tgtttcaatc aaactattaa gcgttgcaag ttgtgccgtt
      481 tgttcatctt gctttgttgc tgtcgccgcc cctgttgcaa gtggtactac tgataaacta
      541 actggaacgg ctgctgctct taattcagca tctgttaaag catctgtttg ttgatttgct
      601 gatgtggcaa gtccggtagg taatgaagaa actgcaactg ttcctgaaac gggttgtgtt
      661 acaggaaaat tacttacttc taccgtaccc gttaccggca aaggatctga tgcacttacc
      721 atcgttgcca caccatcaac accaaactca acctttacca actcatgctg aacaccatta
      781 acctcatctg ttgcaatggt agcaccccct ggagttgtga ctgcgttaat taaaacatta
      841 tctgccatat taaacctgtt gtgccgttgc gcttattatc aaatcatttg aatccttaac
      901 cactgtaaac tgatatttcc ttttcttcat aacttctttt agtaattcgt caaaccttaa
      961 atttatcatt gccatcattt cagctctgaa agtatctatc tcactttttg aactactgtt
     1021 aagaactatc tcaggcttgt aatctttaga attaattaaa tcaatcaatt catctatccg
     1081 gcttaaatca ccattcagct tttcttcaat caacctttca agttcatccc ttactttagt
     1141 aacaacttca acctgccttt caccctcacc acttatcaac tcaccaaata attttatttc
     1201 gtccaaatct tcttttgtcg ccaacccttc aaacctttcc agtaccgggc ttaaatctgt
     1261 ttcttttatt cctctcacgt cccggcctat gtccctgatt tcgtccttta gataatcaat
     1321 cggttcaatt atctcactgt aatcaggttt cagttctttt gtttcttcag gctccaccga
     1381 cttgataagt cctgtctttg gtatcaaccg atcattcgca tctcttttcg ccaccaatgc
     1441 aaccgtacat cggcaattaa tcaccgttgc cgctttgtcc cttctcattc cctgcgcatt
     1501 tggatcccct ggctgcatta atcttacccc gtttctcgga tcaacaaacg gctgattata
     1561 gtcaaccact accccatcca acccccaatg attggcatgg tcttctgggt tgcggcccct
     1621 caccctgtta tcatgccctg aaatccattc cttcatcgtc ttatactcaa acttttccgc
     1681 cgccgccact gccgccacgt tggtagcact atttacttcc gtcctcacaa tccttgccgc
     1741 ttgccgcttc tcaaagcctt tatcccgtat cgcttgcgca atctcctcaa acgttgcgcc
     1801 atctgttaca gctttggata ttagcggtag aaaaaaatct ctcattgtct ttaccgcccc
     1861 aaacgttatt ttttctacca aatgcttgcg gaaataattc gtaacaaaat taatgaagtc
     1921 tggattaaac ccaaaacctt tttcttcctt ccgcaaatct ctgtaaaccc tattggcatg
     1981 gtaaacgcca acatttgaat ataaatcttg tactatctct gttaatgatt ttgatgcaat
     2041 gttatcatca aagtatttag gtgccgaatc aatgccgttt atcttcaagt actcaatcaa
     2101 agaactaata tcttgcttta tcgccttttg cagcttagga taaaatctgc tctcaaacct
     2161 gcgatttaca agttccacca aacgactgta ttttttcctt tcctgcaaat tcatttatca
     2221 atcttttttt taattgcgaa cgcagcattt ccattctggc tttctcaata ctacacctcc
     2281 tttctttctt cgttaccggg tagcgttcaa atattatctt ctcaagatca ttcatcccct
     2341 atcattttgt caatctcatt tgcttgcacc tcactcatcg gttgcttggt tccgtctatc
     2401 cacggctcat caaataaagg gttgtcgttt ttttgcaacc ctaaaatatt taactgctca
     2461 tttggtgaca acatccataa cttattaacc caatctactt tttcacccat gttttcgtcc
     2521 agctctgaaa aaatagtaaa gtcgtaatca acataaatat ttttcccctt gaatccccaa
     2581 tcattgttta atttatgatt aaatgcgtct ctgaaagttg taagcagcgg aaatgcgcac
     2641 ctcgttgtga gtgccttctc cgcttctttt acattgttgt aagttttttg agtaagcccc
     2701 aacaattccg gcggcacacc gtaaatgttg cacaacatta ccgcatccca tttctcactc
     2761 tctattatct gcatatccac cggcgataag cctatgtccg tccaattcat cctgtatgcc
     2821 gtcgctacca tcttgccgta agaatcggca cctgagtact cagggctggt taataatttt
     2881 tttaccttgg cggcttgctc ccccattatt gcaatgttac ccatgtcaac atcttgcggc
     2941 gatatatctg gcgaaatgaa tcccttcacc ccctgatttt taaaggctac cgatgcagcc
     3001 gtcatcgcct cattatttct cgtatatcgt tttacagctg cctttaaagg cgataagcca
     3061 taaagctgag cgccgtttgt tgaccactcg gggtta
//
LOCUS       DAOSZP010000238         3092 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_24832_length_3092_cov_2.675996, whole genome shotgun sequence.
ACCESSION   DAOSZP010000238 DAOSZP010000000
VERSION     DAOSZP010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3092
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24832_length_3092_cov_2.675996"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(84..1217)
                     /locus_tag="PK348_06060"
     CDS             complement(84..1217)
                     /locus_tag="PK348_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HXK65813.1"
                     /translation="MGQQANVNLDYNPQKNTEGLIPFSAPLNSPEVHDDHTVTFRLKA
                     PKAQEVKLAGVAILTALGKGNEQIPFTKGDDGIWTLTVGPLKPDMYAYHFNVDGVQIT
                     DPSNTYAAFTAMPPYSELIVHGSEPAYYDAKNVPHGTVTRHIYHSEVTNGERELYVYT
                     PPGYSKEKYYPVLYLLGGSGELPSNWIYDGRVNFIMDNLLAEGKAIPMIIVIPNNQVI
                     HRNHPKHAELTFDLFEKELRNHVIPLIDEYYNTIKSPKGRALSGLSMGGRHSMYVGFR
                     SLDLFANFGILSAGDVNAESSLAEFLNDPKVNDKIDYLFVGQGTEEANAFLGKRCVAL
                     HDALTKHNIKHEYYVGGYGGHDWATWRHLLYYRFLPNLWRKNN"
     gene            complement(1295..2458)
                     /locus_tag="PK348_06065"
     CDS             complement(1295..2458)
                     /locus_tag="PK348_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012926009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HXK65814.1"
                     /translation="MLKKIIAGLIAISSLHVFSQQSQVIEDFKPSWVNQPGKQYPQVN
                     SEGRVRTQIPAPQAKRVQLDIGAVKYDLIKDTNGVWTGESAPQDEGFHYYQLWIDGAA
                     VPDPGTIFFYGAGRWGSGIEIPAHDQDIYALKDVPHGLVSEVLYFSKITQAWRRCFVY
                     TPPGYETNTNEHYPVLYLQHGSFEDETGWSRQGKANLILDNLIVEKKAVPMIIVMDNG
                     YAFKPQEPHTVTNPTRPAMVFEDVMIKEIIPMIDSRFRTIADREHRAIAGLSMGANQT
                     MRIIMNNLNVFASFGGFSGTSNYPSADDIDPTTFLDGKFSNGKEINQKIKVFFLSLGT
                     KEPNPFPGSVGAFRKMLEKQGIKYTFYESPGTAHEWQTWRRSLYQFAQFIFKK"
     gene            complement(2474..>3092)
                     /locus_tag="PK348_06070"
     CDS             complement(2474..>3092)
                     /locus_tag="PK348_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010527909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HXK65815.1"
                     /translation="CSGSAIALTPETQTCDAIVQAWYAGEAGGQAVADVLFGDYNPSG
                     KLPITFYKSSDQLPDFENYSMKGRTYRFMNDALFPFGFGLSYTTFKMGEAKLNKMQIS
                     ANEAIEITVPVTNAGKRDGTEIVQVYVRKVNDADGPIKTLRGYQRVDVPAGKTRNAVV
                     TLPYSSFEFYDRKSGKVTVTAGEYEIFYGNSSDSKDLKSLKIRVQ"
BASE COUNT          939 a          655 c          546 g          952 t
ORIGIN      
        1 aatttgtttt ttacaaccag cactaacaaa tagcattatt gcaaacaaaa tgatatttat
       61 tttcttcatt actattattt tttttaatta ttttttcgcc atagatttgg cagaaaccga
      121 taatacaaaa ggtgtcgcca tgtagcccaa tcatggcctc catatcctcc tacataatat
      181 tcatgcttta tattatgttt tgtcaaagca tcatgcaaag caacacaacg ttttcctaga
      241 aatgcatttg cttcttcagt tccctgacca acaaataggt aatcgatttt atcatttact
      301 ttcggatcat ttaaaaattc agctaatgaa gattcggcat taacatcacc ggcactgaga
      361 ataccaaaat tggcaaacaa atcaagagaa cgaaaaccta catacatact gtgacgacca
      421 cccattgaca aacccgataa tgcacgtcct tttggagatt taatggtatt atagtattca
      481 tcaattaaag gaataacatg gtttcgaagt tctttttcaa aaagatcaaa tgtcaattca
      541 gcatgttttg gatgattgcg atgaattacc tgattgtttg ggataacaat tatcatcggt
      601 attgccttcc cctctgccaa caaattatcc ataataaaat taaccctgcc atcatatatc
      661 caattagaag gaagctcacc acttcctcct aaaaggtata atactggata atatttctcc
      721 ttactatatc ccgggggcgt gtaaacatat agctcacgct caccattagt aacctcagaa
      781 tgataaatat ggcgcgttac tgtgccatgc gggacatttt tggcatcata gtatgctggc
      841 tcgcttccat gcacaatcaa ttcactataa ggcggcatag cagtaaaagc tgcataagtg
      901 ttggacgggt cagttatctg aaccccgtca acattaaaat gatatgcata catatccggt
      961 tttaatggcc ctacggttaa agtccaaata ccatcatcac ctttagtaaa tggtatctgt
     1021 tcattaccct tacccagggc agttagaatt gccaccccag ccaatttaac ttcttgagct
     1081 tttggagctt tcaaacgaaa tgtaactgta tggtcatcat gtacttcagg tgaattcaat
     1141 ggtgcactga acggaataag tccctcagtg tttttctgag ggttataatc aagattgaca
     1201 ttggcttgct gacccataca aacgaatgat atcagcatga aaaacaagga tagtatttta
     1261 aatattcttt tatttttcat ttttcaactt taaattattt tttgaatata aattgtgcaa
     1321 attgatacaa tgatctgcgc catgtctgcc attcatgagc agttccgggc gactcgtaaa
     1381 aagtatattt aataccttgc ttttcaagca tcttacgaaa tgcgcctacc gacccaggaa
     1441 atggattagg ctctttggta cctaagctaa ggaaaaaaac cttaatcttt tgattgattt
     1501 ctttaccatt actgaattta ccgtctagaa aagttgtagg atcaatatca tcagcgcttg
     1561 gataatttga agtaccacta aatccgccaa aagatgcgaa gacgttaaga ttattcatta
     1621 taatgcgcat ggtctggtta gctcccattg acaaaccagc aattgcacgg tgctctctat
     1681 cggcgatagt acgaaaacgc gagtctatca ttggaataat ttccttaatc attacatcct
     1741 caaaaaccat tgccggacga gtaggattcg ttacagtatg aggttcctgt ggtttaaaag
     1801 cataaccatt gtccattaca ataatcattg gcacagcttt tttctcaaca ataagattgt
     1861 cgagtatcaa attggcttta ccctgcctcg accagcccgt ttcatcttca aaacttccat
     1921 gctgaagata taatactgga tagtgctcat ttgtatttgt ttcatatcca ggaggagtgt
     1981 aaacaaaaca tcgacgccat gcctgagtaa ttttcgagaa atataaaact tcacttacaa
     2041 gtccgtgagg aacatctttc agagcatata tatcctgatc atgagcagga atttcaatac
     2101 cactacccca acgaccagcg ccatagaaaa agatagttcc gggatcagga acagcagctc
     2161 catcaatcca tagttgataa taatggaaac cttcatcctg aggagcagat tcgcctgtcc
     2221 acacgccatt agtatccttt atcaaatcat attttacagc accaatatca agctgcaccc
     2281 tttttgcctg gggtgcagga atttgtgttc ttactcgtcc ttcagagtta acttgtggat
     2341 attgctttcc tggttgatta acccacgaag gcttaaaatc ttcaataact tgagattgtt
     2401 gagaaaatac atgcaaactt gatatggcaa ttaatccagc aattattttc ttaagcataa
     2461 tcatttattc tgtttattga actctaattt tcaaactttt taaatctttg ctatcggagc
     2521 tatttccata aaaaatttca tattcgccag ccgttaccgt taccttaccg cttttacggt
     2581 cgtaaaattc gaaagacgag tatggtaacg taaccacagc atttcgggtt ttaccagccg
     2641 gcacatcaac tcgctgatag cctctgagcg tttttatggg gccgtcggca tcattcactt
     2701 ttcggacgta cacctgaacg atttcggtac catcgcgttt gccagcattg gtaacaggga
     2761 cagtaatctc tatagcttca ttagcggata tttgcatttt atttagctta gcttcaccca
     2821 ttttaaatgt tgtgtagctt agtccaaaac caaacgggaa aagagcatcg ttcataaagc
     2881 ggtaggtacg acctttcatt gaatagtttt cgaaatcggg aagctggtcg gagcttttat
     2941 aaaatgtaat tggtaatttg cccgatgggt tgtagtcgcc aaaaagcaca tcggccacgg
     3001 cctgaccacc agcctcacct gcataccatg cctgaacaat agcatcgcaa gtctgggttt
     3061 cgggggtgag tgcaatagcc gagcctgagc aa
//
LOCUS       DAOSZP010000239         3088 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_24877_length_3088_cov_3.377514, whole genome shotgun sequence.
ACCESSION   DAOSZP010000239 DAOSZP010000000
VERSION     DAOSZP010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3088
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24877_length_3088_cov_3.377514"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="PK348_06075"
     CDS             <1..275
                     /locus_tag="PK348_06075"
                     /inference="COORDINATES: protein motif:HMM:NF020133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HXK65816.1"
                     /translation="LLYKSHQSLSVYYEVSIPLIDEMVEWASDIDGVYGSRLMGGGFG
                     GCTISMVALYAVEHFTRTIAQKFKKRENRIPQIYECSIEDGTRRIE"
     gene            362..1075
                     /locus_tag="PK348_06080"
     CDS             362..1075
                     /locus_tag="PK348_06080"
                     /inference="COORDINATES: protein motif:HMM:NF019456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HXK65817.1"
                     /translation="MKKNTLGKIQKNTLHQQIVSLLAKRIINQMQPGDKLPSERDIAA
                     DLDVNRATVREALKKLETLGLIEIRHGEGIFIKDYLTSGNLELFKMIIYLNDTIPLSI
                     LQNLLEIRRIIVPQMAANASENITREELQQFEAIVTSHDTILEKDLAVHQLIAKASRN
                     LLYIFILNFFNQIFRDFGYLYFENPDNQNRSELFHRELLEAFKAKNSKKAYTITHSVL
                     EYTEKRIFAYYSQIYNNKG"
     gene            1080..2708
                     /locus_tag="PK348_06085"
     CDS             1080..2708
                     /locus_tag="PK348_06085"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HXK65818.1"
                     /translation="MKRFIEENTKEQFDCIIVGGGITGAAVAYEAASRGLTVALLEKN
                     DFGWATSAATSKLIHGGLRYLNYFEFGLVRESLRERRILENIAPNLVYPFPFMIPNYN
                     RLENNRWVLKIGMILYDILSYDKGFTWDKSKKIPMHFTLSKEKALQEEPNIRPEGLTA
                     ATVYYDCQSIFPERLTLAFIKSAVQYGAKVANYAEVVNFVYTNNAITGVIVHDKLTGR
                     EVTINGNLIINCGGPWADIILKKAENGKTKHQIRRSEGIHIITKPMVNTYAVGLMPRK
                     GRHFFLIPWRGYSLIGTTDKEYIGNPDEYTVTKQSIQELLDDVNSAFGDGNLTFKDIR
                     FAYGGLRPLVEDQVEGTYQSSRRYEIYDNAEEGVNGLITVEGGKYTTSRNLAVNVMKL
                     VEKKIQRKLPPSVTHTQYLAGCTIKDLEAFFNTVYNSYPSFNKNTLTVLAKYYGSELD
                     ALMSIANSNKKYREIVTHDGEILAEVIYAIRHEMARTLSDIVFRRTGICTAGNPGNDV
                     LNLVAETAAKELKWDEKELKRQLKSVQQRLQLPK"
     gene            2775..2963
                     /locus_tag="PK348_06090"
     CDS             2775..2963
                     /locus_tag="PK348_06090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65819.1"
                     /translation="MVEIHPLNNFPNYAPVLAYWSYNQWYSKRDIPFTALVQSYIERA
                     RNTTVPWHGLPLMKKCFP"
BASE COUNT         1045 a          605 c          610 g          828 t
ORIGIN      
        1 aacttctcta taaatctcat caaagcttaa gcgtttacta tgaggtcagt atccctctaa
       61 tagatgaaat ggtagaatgg gccagtgata ttgatggagt ctatggctca cggcttatgg
      121 gagggggatt tggtggatgt actataagca tggtggcatt atatgcggta gaacatttta
      181 caagaactat cgcccagaaa tttaaaaaga gggaaaacag gatcccccaa atttacgaat
      241 gcagcattga agacggaacc cgccgaattg aataaaaatt ttttataaat attttgattt
      301 actataaact catggtatac tattagtggt tgaaccactg aaccactata aaggtgttgc
      361 tatgaaaaaa aatacattag gaaaaataca gaaaaacacc cttcaccagc agatagtttc
      421 cctgcttgca aaacgcatca ttaatcagat gcagcccggg gacaaactgc catccgaacg
      481 ggatattgct gctgatttag atgtcaacag ggctaccgtg cgtgaagcat taaagaaact
      541 ggagacactg gggcttattg aaatccgcca cggtgaaggc atctttatta aagattacct
      601 taccagcggc aatttagaac ttttcaagat gataatatac cttaatgata ctattcctct
      661 ttcaatactg caaaatcttc ttgaaattcg aaggattatt gtcccccaga tggcagccaa
      721 tgcttcagaa aacatcacca gagaagaatt acagcaattt gaagcgatag taaccagtca
      781 tgatacgatt cttgaaaaag acttagctgt tcatcagctt attgctaaag caagcagaaa
      841 tttgctgtat atattcattt taaatttctt caaccagata ttcagggatt ttggctacct
      901 gtattttgaa aatcctgata accagaaccg ctcagaacta tttcaccgtg aacttctaga
      961 agcctttaaa gctaaaaaca gcaaaaaggc atacactata acacatagtg tactggaata
     1021 cactgaaaaa agaatttttg catactattc gcaaatctat aacaataagg ggtaatgcta
     1081 tgaaacgttt tattgaagaa aatactaaag aacaatttga ttgtatcatt gtaggaggtg
     1141 gcattaccgg cgctgctgtt gcctatgagg ctgcatctcg aggacttact gttgcacttc
     1201 ttgagaaaaa cgattttgga tgggcaacct ctgcagcaac ctcaaaactt attcacgggg
     1261 gtctgcgata cttaaattac tttgaatttg gacttgtgcg tgaatcattg cgagaaagaa
     1321 ggatactgga aaatatcgca ccaaaccttg tatatccttt cccattcatg atacccaatt
     1381 ataacagact ggaaaacaac cgatgggtgc ttaaaattgg aatgattctg tatgatatac
     1441 tatcctatga taaaggcttt acctgggata aatcaaaaaa aattcctatg cactttacat
     1501 tgagtaaaga aaaagccctg caggaagaac caaacattcg ccctgaaggt ttaactgcag
     1561 ctaccgtgta ttatgattgt cagagcatat ttccagaacg cttaacttta gcatttatca
     1621 aatctgccgt acagtacggc gctaaagtag ctaactatgc tgaagttgtg aattttgtat
     1681 acaccaataa tgcaatcaca ggtgttatcg ttcatgacaa acttactggc agggaagtaa
     1741 ccattaacgg caatctcatc atcaactgcg gtggaccatg ggctgatata atcctgaaaa
     1801 aagcagaaaa tgggaagaca aaacatcaaa tacggcgatc agaaggaatt cacataatca
     1861 caaaacctat ggtaaataca tatgctgttg ggcttatgcc acgaaaagga aggcattttt
     1921 tccttattcc ctggagaggg tactctctca ttggaaccac tgataaggaa tatattggca
     1981 accccgatga gtataccgtc acaaaacaga gtatacagga actgcttgat gatgtaaaca
     2041 gtgcatttgg tgacggcaac ctcacattta aagacattag atttgcctat ggcgggttgc
     2101 gtccgctggt tgaagatcag gtagaaggaa cataccagtc atcacggcga tatgaaatat
     2161 acgataatgc tgaagaaggt gttaatggcc ttattactgt tgaaggtggt aaatatacta
     2221 ccagccgtaa cctggcggtt aatgtcatga agcttgttga aaagaaaata cagcgcaaac
     2281 ttcccccttc agttacccac acacaatatt tagcaggatg cacaataaaa gaccttgaag
     2341 cattctttaa tacggtatac aattcatatc cttcatttaa taaaaacact cttacagtcc
     2401 tggcaaaata ttacggatca gaattagatg cattgatgag catagctaat tcaaacaaga
     2461 agtacaggga gatagttact catgacgggg aaattcttgc agaagttatt tatgcaatac
     2521 gccatgagat ggcacgcact ctttcagata tcgtattcag gcgaacaggt atctgcaccg
     2581 caggaaatcc tggcaatgat gtactcaatt tggtagctga aactgcagca aaagaactta
     2641 aatgggatga aaaagaattg aaacgacaac tcaaaagcgt acaacaacgg cttcaactcc
     2701 ccaaataatt attgaaatca atacctattg gtgctatctc tggtactacg taacaataat
     2761 acgaggtagc accaatggtt gaaatacacc ctcttaataa tttccccaac tacgcaccgg
     2821 tactggcata ctggtcatat aatcaatggt acagtaaaag ggatataccc tttactgcgc
     2881 tggtccagtc atatatagaa cgcgctcgca atacaacggt accctggcat gggttgccat
     2941 taatgaaaaa atgcttcccg taggcatggc ttcactgaaa aataatgacc tgtggtcccg
     3001 tactgacctc aatccatggc ttgcatcact gtatattgtt cccgaatatc gcttccaggg
     3061 aattggagac aggataatca aagtaata
//
LOCUS       DAOSZP010000240         3072 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_25027_length_3072_cov_3.288697, whole genome shotgun sequence.
ACCESSION   DAOSZP010000240 DAOSZP010000000
VERSION     DAOSZP010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3072
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25027_length_3072_cov_3.288697"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..468)
                     /locus_tag="PK348_06095"
     CDS             complement(<1..468)
                     /locus_tag="PK348_06095"
                     /inference="COORDINATES: protein motif:HMM:NF013296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II D-tagatose-bisphosphate aldolase,
                     non-catalytic subunit"
                     /protein_id="HXK65820.1"
                     /translation="MSILDLRPENFKKAFPDSKVMHVNGKSIIQSAMKEGNHWVVMAV
                     NTRHEIAIPGIIKASMELQAPVIYELALSESDLTGGYTGLTPKDFITLVVTLNEKMGN
                     VGEKAVPFTCHRDHTTVQEITRKAFDKADSLIKASIEAGYGSFSIDCSFLPMEQ"
     gene            complement(563..1396)
                     /locus_tag="PK348_06100"
     CDS             complement(563..1396)
                     /locus_tag="PK348_06100"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXK65821.1"
                     /translation="MNTYPYPIIDFHVHLFPDRMFDAIWKSFVKDYGWDVMYHLYYKE
                     CIAYLKERGIEKIVYSNYAHKEGVAKHLNDWNLKVLDEQDNVYCFGAYHPDDDIAIAE
                     DFITHPKVLGFKLQLLVQKCYPYDTRLFPLYELVQHYKKRILMHVGTGPVGNEFVGIG
                     GFKKLLRQYPDIPVTIAHMGAYEYDEFLELLDDYPHLMMDTAFVFLPDYEGAFHKDPE
                     VLEKYQDRILYGSDFPNIIFPRELEIETLMKYGLSKTTLENILYNNGNRIIRQIVHDD
                     R"
     gene            complement(1393..1509)
                     /locus_tag="PK348_06105"
                     /pseudo
     CDS             complement(1393..1509)
                     /locus_tag="PK348_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004478830.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            2469..>3072
                     /locus_tag="PK348_06110"
     CDS             2469..>3072
                     /locus_tag="PK348_06110"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HXK65822.1"
                     /translation="MTQHQNVNYEKEFEEYLNREKHLALMVLNRAKKFGDSKIAVRHK
                     AYGTWEAFTWKQFGDLIESAARGLLLFGVKEFEFVGIFSNNRVEWAVVDYASFAIRAC
                     SVPVYATNSAKELEYIVNDCGIRILFVEKQEHYDKAMSIIDSAKSLEKIIVFDRNTKI
                     ASDPRVMLFDDFIAMGKSKSIYAQLQERLSKVQSDDLSTLI"
BASE COUNT         1034 a          585 c          510 g          943 t
ORIGIN      
        1 ctgttccatt ggcaaaaaag aacaatctat tgaaaaggaa ccatatcccg cctcaataga
       61 agcttttata agggaatcag ctttatcaaa cgctttacgg gttatttcct gtacggtggt
      121 atgatctctg tggcaggtaa atggtacagc tttttcacca acatttccca ttttttcatt
      181 taatgttact accagtgtaa taaaatcttt aggtgtcaat ccggtatatc caccggttaa
      241 atcactttcg gataatgcca gttcataaat caccggtgcc tgtaattcca tggaagcttt
      301 aataatccca ggaatagcaa tttcatgccg tgtattaaca gccatcacca cccagtggtt
      361 tccttctttc attgcagatt gtataattga ctttccgttt acatgcatca ctttggaatc
      421 agggaatgct tttttaaaat tttcaggacg taaatccaga atactcatat attcctccgt
      481 tattatttat ataattgatt tatgaaaacc tatcagtatc aaaaatttaa tcaagcataa
      541 tttttaacac taaaaaatat tctcacctat catcatgtac tatctgtctt ataattctgt
      601 ttccattgtt atacagtatg ttttccaatg ttgttttgct taatccgtat ttcattaatg
      661 tctcgatctc caactcacgg ggaaagatta tattgggaaa atcagaacca tacaaaattc
      721 tatcctgata tttttccagt acctccggat ctttatggaa agcaccttca taatctggta
      781 aaaacacaaa cgctgtatcc atcataagat ggggataatc atctaaaagt tctaaaaatt
      841 catcatattc atacgcaccc atatgggcga tagttaccgg tatatcagga tattgcctca
      901 gtaatttctt aaatccgcca atcccaacaa attcgtttcc caccggaccg gtgccaacat
      961 gcatcaggat gcgcttttta tagtgttgta ccagttcata cagtgggaaa aggcgtgtat
     1021 cgtaaggata acatttctga actaaaagct gtaacttaaa ccccagtacc tttggatggg
     1081 taataaaatc ttcagcaatt gcgatatcat catcaggatg atatgcgcca aagcaataca
     1141 cattgtcctg ttcatccaga accttcaggt tccaatcatt taaatgtttt gcaacacctt
     1201 ctttatgagc ataatttgaa tacacaattt tttcaatacc acgttcttta agatatgcaa
     1261 tacattcctt ataatacagg tgatacataa catcccaacc atagtctttc acaaaagatt
     1321 tccagatagc atcaaacatg cggtcaggga aaagatgtac atgaaaatca atgattggat
     1381 aaggatatgt gttcattttt taccatattc tttgcgatat tgttttggtg acatcccggt
     1441 catttttgaa aaaaccgtac agaatgtggt attggaatta aaccccacag caacaccaat
     1501 agataatata tctagccctt taatccgtga acgttcatat cgtgccttca tagcctcact
     1561 ttttaaaagc ttgttataat caggatgacg ctgtgtaaca aggtacacaa aagtcattgc
     1621 tgtattggct atgatcactg cccattccac tacgtgtaat gaaacaaatg ccccatatac
     1681 acatattgca ttggatacca ctatggataa cgcaaagatg tagcccatcc gtgaaacgcc
     1741 aggcacagta ttccattttt tcctattcca tacgtatgcc atagttatca aattaggcaa
     1801 cataaacgta ataagataga tattgggaat tataaaaagc aggtacacac ctttatcata
     1861 aagcgatagt tcatagaatc tgtcacttag cagtggtaat ccagggaaac atgctgaacc
     1921 gcatgaaatg cccttgataa acaaaggtac cagcaaatag aataaagaaa ttatggatgg
     1981 caacaataca atataataga ttttctttac atcaaagagg cttgttatta tccaggtgta
     2041 tcgcatcacc ataagtgggg gaacaataaa aattacggga attgaagcaa tacttacata
     2101 gtacccaata gcggaactat cgaacagacc tgacgaataa atgcatattt gcaaaagcca
     2161 aattcctaag ccaaagaaag aaactgcata tagtttatta atggttacaa ttttcttctg
     2221 cacaaactgc cccagggcaa taatcactga aaaaactgca ccatagaaca taaaaaaatg
     2281 taatactgat tcactgctca taaatactca aatttatata attataattt taaaattatt
     2341 aaactttcaa agttataact ctgaaagact tttaaacata taaatgatat actctgaata
     2401 gcattataca ataccatccg gtcgttatct ggctttaccg gatgtaacct atcatgggag
     2461 gttatattat gactcagcat caaaatgtca attatgaaaa ggaatttgaa gaatacctta
     2521 atagggaaaa gcaccttgct ctgatggtgc ttaacagggc taaaaaattt ggtgattcaa
     2581 aaattgctgt acgccacaaa gcctatggta catgggaagc atttacctgg aagcaatttg
     2641 gtgatctaat tgaatcggca gccagaggat tgctcctttt tggagtaaag gaatttgaat
     2701 ttgttggtat tttttcaaat aacagagtgg aatgggctgt tgttgattat gcttcatttg
     2761 caataagggc atgctctgta ccggtttatg ccacaaactc agccaaagaa cttgaatata
     2821 tagtcaacga ttgcggaata cgcattcttt ttgttgaaaa gcaggaacac tatgacaaag
     2881 caatgagtat aattgattca gcaaaaagcc ttgaaaaaat cattgttttt gaccgtaata
     2941 caaaaattgc cagtgaccca cgtgttatgc tgtttgatga ctttattgct atgggcaaaa
     3001 gtaagagcat ttatgcacaa ttacaagaac gcttatcaaa agtgcaaagt gatgaccttt
     3061 caacacttat tt
//
LOCUS       DAOSZP010000241         3071 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_25041_length_3071_cov_3.458886, whole genome shotgun sequence.
ACCESSION   DAOSZP010000241 DAOSZP010000000
VERSION     DAOSZP010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3071
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25041_length_3071_cov_3.458886"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1136)
                     /locus_tag="PK348_06115"
     CDS             complement(<1..1136)
                     /locus_tag="PK348_06115"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HXK65823.1"
                     /translation="MNKHYCILDCYVDEPACFGVPPFVSPYPRYVYGALIDAGIYEDE
                     IVYLTIDSLRASEYRLPHNFDHVFLIGGAIVPGKYLGFKIGTIPEITKIIATNKRQKF
                     AIGGMIARLVKDFPNTTLVTGDIEQFAYSVATGNDPVTDNYRTYKDLARWAFYGSKLI
                     TLHPQFPNLICEIETYRGCPRRNHCSFCAEGLLQSVDFRDEEDIVHEIDTLVAQGISR
                     FRIGRQADIIAYKSRLQDFVHGFPRPEPDAVINLFTELRKRINEGHINMLNIDNANPG
                     TIYYFPEESQTIIDCIAHTVTPGDTMPLGVESFDPAVIQKNNLKLHKDQVLQVVQLIN
                     DAGGKTINGIHRLLPGINLIHGLIGETADTFKINYESLVEILDKG"
     gene            complement(1129..1674)
                     /locus_tag="PK348_06120"
     CDS             complement(1129..1674)
                     /locus_tag="PK348_06120"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HXK65824.1"
                     /translation="MNIPDMTTCLSLLQQYGTLPNVLEHSLQVHKVARAIVDHLNGSV
                     TINRDLVCAAALLHDITKSRALKTGEKHDISGKELLESLGYPEIADIVGQHVFLRDTS
                     PTSPLNEADIVYYADKRVMHNEIVTVDQRIEDLLKRYGTTQERCNKILENKDIILIIE
                     KKLTRHLVKPLEEIIASLKHE"
     gene            complement(1733..2440)
                     /locus_tag="PK348_06125"
     CDS             complement(1733..2440)
                     /locus_tag="PK348_06125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65825.1"
                     /translation="MIAIINPDTPQAIITGLMHHGFSPVTIPLCPYVERPVAGHPDIQ
                     LCIIGNNIIYEPRIEKTFLQVLSQSTYTLIPGKSHLQATYPYDCAYNCAYTGSIAFHN
                     TKVTDSSIKQILTPYSVPLIHVNQGYTRCSTCIVDSHAIITSDTSIHNAAVNNGIDSL
                     LIQPGYIELPGYKYGFIGGASCMCSDTMYFTGRLSHHPDYSNIVNFIAIHNKKIVFLS
                     DLPAIDTGSIFFIDMNR"
     gene            complement(2437..2832)
                     /locus_tag="PK348_06130"
     CDS             complement(2437..2832)
                     /locus_tag="PK348_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019235277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HXK65826.1"
                     /translation="MKNKFKNIFFNFRDTVGALLVAIKHPDVSLWSKIIIAVAIAYAL
                     SPVDLLPDFIPFIGYLDDAVIVPFLFFIAIKSIPQSVYEECKVQAHTAFKKSPGFIVL
                     GIVIVILLWAFIGFFMYALVSGILRLLKL"
BASE COUNT          946 a          562 c          625 g          938 t
ORIGIN      
        1 cctttatcaa gaatttccac cagtgattca tagttgattt taaatgtgtc agcagtttca
       61 ccaataagtc catgaataag attaatacca ggcaaaagcc tgtgaatccc attaatagtt
      121 tttccacctg catcatttat taattgtaca acctgtaaaa cctgatcttt gtgcaattta
      181 aggttgttct tttgaataac cgctggatca aatgactcaa cgcctaacgg cattgtatcg
      241 cccggtgtta cagtatgagc tatgcaatca ataatagttt gcgattcttc aggaaagtaa
      301 tagatggtac caggatttgc attgtcaatg ttgagcatat tgatatgccc ttcattgatg
      361 cgctttcgca attcagtaaa aagattgata actgcatcag gttctggacg cgggaaccca
      421 tgaacaaagt cttgcagacg cgatttatac gcaataatat cagcctgcct ccctatcctg
      481 aaacgggaaa tcccctgagc aaccagtgta tctatttcat gtacgatatc ttcttcatct
      541 ctgaaatcaa cggattgcag taatccttca gcacaaaatg agcagtgatt gcggcgtggg
      601 catcctctat aggtttctat ctcacagata aggttgggga attgtggatg aagtgtgatc
      661 agtttactgc cataaaacgc ccagcgtgcc aggtctttgt atgtgcgata gttatcggta
      721 acaggatcat tgccagtagc aacggaatat gcaaactgtt caatatcacc ggttaccagt
      781 gttgtattgg gaaaatcctt aaccaggcga gctatcatcc cgccaatggc aaatttctgt
      841 cgtttgtttg ttgcaatgat ttttgttatt tcagggatag tacctatttt aaagccaagg
      901 tatttgccag gaactatcgc ccccccaatt aaaaaaacat ggtcaaaatt atgtggcaac
      961 ctgtattcag acgcacgaag ggaatcaatg gtaagataca caatttcatc ttcgtagatg
     1021 ccggcatcga tgagtgcacc atacacatag cgcgggtagg gcgaaacaaa cggtggtaca
     1081 ccaaaacacg ccggttcgtc aacgtaacaa tcaaggatgc agtagtgctt attcatgctt
     1141 taatgatgcg ataatctctt ccagtggttt tacaaggtgt ctggttaatt ttttttcaat
     1201 gatcagtata atatctttgt tttcaagaat tttgttacag cgttcttgtg tggttccata
     1261 gcgtttgagt aaatcttcaa tgcgctggtc aacggtaacg atttcattat gcataacgcg
     1321 tttgtctgca taatatacta tatctgcttc attgagcggt gaggtgggtg aagtgtcgcg
     1381 taagaataca tgctgcccta caatatcggc tatttctgga tatcctaacg attctaaaag
     1441 ctctttgcct gaaatgtcat gcttttcacc tgttttcagt gcacgggatt tggtgatgtc
     1501 atgaagtagg gctgcagcac atactaaatc acgattgatt gtaacactgc cgttgagatg
     1561 atcaacaatg gcacgggcaa ctttgtgtac ctgcaatgaa tgttccagca cattgggcaa
     1621 agttccatat tgttgcagta gtgataaaca tgttgtcatg tcaggaatat tcatgaatgg
     1681 tatccatggt gttttatgat taatgttatg tacacaccat atgaatagca tgttatctat
     1741 tcatgtcaat aaaaaagatt gagccagtat caattgcagg aagatcggat aaaaagacaa
     1801 tcttcttgtt gtggattgca ataaagttga ctatattact gtaatcaggg tgatggctta
     1861 aacgccctgt gaagtacatg gtgtcagagc acatacaact tgccccacca ataaaaccgt
     1921 atttgtagcc tggcagttca atatatcctg gttgtatgag cagtgaatca atgccattgt
     1981 ttacagcagc attgtgaatg gaagtatctg atgtaataat ggcatgtgag tcaacaatac
     2041 aggtagagca tctggtgtat ccctgattta catgaatcag agggacggag tatggagtta
     2101 agatttgttt tatggaacta tcggttactt ttgtattatg aaacgctatg ctgccagtat
     2161 aagcgcaatt atatgcacag tcgtaagggt atgttgcctg caggtggctt ttgcctggta
     2221 tcagtgtata tgttgattgc gataatacct gtaaaaatgt cttttcaata cgtggttcat
     2281 aaataatatt attgcctatg atgcacagct gtatatcggg gtgccctgca acaggccgtt
     2341 caacgtaagg acacagtgga attgttacag gtgaaaatcc atgatgcatt aatcctgtta
     2401 taatagcctg aggcgtgtca ggattgataa tagcaatcat agcttcaaaa gccttagtat
     2461 tcctgaaact aaagcataca taaaaaaacc tatgaatgcc cacaaaagaa taacaatcac
     2521 aatccccagc acaataaatc ccggtgactt tttaaatgca gtatgcgcct gaaccttgca
     2581 ttcttcatac actgattgtg ggatagattt tatggctata aaaaataaaa aaggaactat
     2641 aacagcgtca tccagatatc caatgaatgg aataaaatca ggtagtagat ccacggggga
     2701 cagagcatac gctattgcca ctgcaatgat gatttttgac catagtgaaa cgtcaggatg
     2761 cttgatagca accagcaatg ccccaacggt atctctgaaa ttgaaaaaaa tgtttttgaa
     2821 tttgtttttc atacgttgta tatggtaaaa cactgtaaac gatgtcaata aaagttattg
     2881 aataagtttt taaatattat attatatata cagaagacgt tttgatacaa ttttattgct
     2941 atttttggca aaatcatcag tgtggctgct aatacatttg ttaaacaata atttttaaac
     3001 agcgtaagcc tgtaagtatt aatgtaacta acaactaatt aaaataatca aacagcataa
     3061 taaaatccat a
//
LOCUS       DAOSZP010000242         3068 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_25073_length_3068_cov_3.404912, whole genome shotgun sequence.
ACCESSION   DAOSZP010000242 DAOSZP010000000
VERSION     DAOSZP010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3068
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25073_length_3068_cov_3.404912"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="PK348_06135"
     CDS             complement(<1..228)
                     /locus_tag="PK348_06135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65827.1"
                     /translation="MNLLYYLPWLSFVLVVASVILAVKSFKKENLLKAAHYSYYGATV
                     VIGFMLVALTIAFVLNTFQFLYVYQHSSVDLP"
     gene            complement(228..599)
                     /locus_tag="PK348_06140"
     CDS             complement(228..599)
                     /locus_tag="PK348_06140"
                     /inference="COORDINATES: protein motif:HMM:NF015085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HXK65828.1"
                     /translation="MKKSNIIIVSIIVVCGAVALFSFKGILTPYVSFDEAAKGEAYVQ
                     VLGKLVKSVPVQNSADGFIFTIEDDNAKRLTVIHKGSKPLNFEHATSVVAIGTYNKEK
                     QIFEADKILVKCPSKYTKENK"
     gene            complement(586..759)
                     /locus_tag="PK348_06145"
     CDS             complement(586..759)
                     /locus_tag="PK348_06145"
                     /inference="COORDINATES: protein motif:HMM:TIGR04391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CcmD family protein"
                     /protein_id="HXK65829.1"
                     /translation="MKLLFMVTIAQNITQIEKSPTTVLFWVSAVVWIGIVCYLLLLHN
                     KVKKLEKRLNEKK"
     gene            complement(756..1409)
                     /gene="ccsA"
                     /locus_tag="PK348_06150"
     CDS             complement(756..1409)
                     /gene="ccsA"
                     /locus_tag="PK348_06150"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HXK65830.1"
                     /translation="MVLIIIDALLMLATLYLIYIWVPPAAILGETSRIVYLHVPLAWV
                     STLAFIVAGLASIQYIRKNAIEYIHCARSSVHIGFFFSILTVITGSLWAKVMWGSYWN
                     WDPRETSITLLLLVYIAYFSLHQIVHTNPKGAMLESVYLVIAFVASPFLIFVVPRLYP
                     SLHPEPVINTQGAILLDTEMRLVLIISVVTFTLFYAIVLKLLLKVYKLEYVCTEDVS"
     gene            complement(1439..2098)
                     /locus_tag="PK348_06155"
     CDS             complement(1439..2098)
                     /locus_tag="PK348_06155"
                     /inference="COORDINATES: protein motif:HMM:NF015344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HXK65831.1"
                     /translation="MIKGLVRNIVKDLRIEFRSKVVISLILSFSVIVTISIGFASGGI
                     IGSPMVHAILLWVIIFFSGMNTSHLFYREIEEHTALFNLMYYSPEGIFVSKLIVNCII
                     MVAVAAIAGLLYLFVMDVSVYNVSGFAIMVLAGSSALAVCTTIIAAITALVQARGGLF
                     TILSFPITLPIITVVIKSTTQCFSKEIYSSWSAILFLLAFSTLLGVVSYILFPYIWRE
                     F"
     gene            complement(2076..2687)
                     /locus_tag="PK348_06160"
     CDS             complement(2076..2687)
                     /locus_tag="PK348_06160"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXK65832.1"
                     /translation="MFTIKAHNISKRYSYRLFSNISCSVSTGESVAIVGPNGSGKSTL
                     LKILGNIVSPDSGRVEYFDKMESIPAHTVIHYAGIVAPYIQCYNHLTLTENIIFAFGS
                     VTDDAIELLHRLDMYQHKDKVLHTYSTGMQQRCKIAMALLKKPKVLLLDEPGSNLDDS
                     GRKILFELIKEIQPFTAIIMATNDSREAKLCHRSIVLDKGIGA"
     gene            complement(2694..>3068)
                     /locus_tag="PK348_06165"
     CDS             complement(2694..>3068)
                     /locus_tag="PK348_06165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c554 family protein"
                     /protein_id="HXK65833.1"
                     /translation="RQMWLEIVILNQYGKEVFSIGKADKRGYLPDNTIIFKTIFGDGK
                     GKPVVNIAKAKEILFDNRISPKESATNTIILPKGLKGQYSIQVRLLYRLADQKTVDEV
                     MKDKAVKLPVIEMATAKKEIVL"
BASE COUNT          996 a          643 c          524 g          905 t
ORIGIN      
        1 cggcaaatcc acagatgagt gttgatacac atataaaaat tgaaatgtgt tgagcacaaa
       61 cgcaatggtt aacgcaacaa gcataaaccc tataacaact gttgcaccat agtaactgta
      121 atgtgctgct tttaacaggt tctctttttt aaacgattta acagctaaaa taaccgaggc
      181 cactaccagc acaaaagaaa gccatgggag gtaatacagc agattcatta tttattctcc
      241 ttggtgtatt tagaagggca ttttaccaga attttatctg cttcaaatat ctgtttttct
      301 ttattatagg ttccaattgc aacaacgctt gtggcgtgtt caaagttaag cggcttggat
      361 cctttatgaa tcacagtcaa tcgtttggca ttatcatcct caatggtaaa tataaagccg
      421 tcagcagaat tttgcaccgg aactgacttc accagttttc ccagtacctg cacgtatgct
      481 tcaccttttg ccgcctcatc aaatgataca tacggcgtta atatcccctt aaaagaaaaa
      541 agcgcaacag caccgcatac tacaatgata cttactataa taatattact ttttttcatt
      601 gagcctcttt tccaattttt taaccttatt atgcaaaagc agcaagtaac acacaatgcc
      661 aatccacaca acggcggata cccagaatag cactgtagta gggcttttct ctatttgtgt
      721 tatgttttgt gcgatagtta ccataaaaag cagtttcatg atacatcctc agtacataca
      781 tattctagct tatatacttt tagcaaaagt tttagcacaa ttgcataaaa aagtgtaaac
      841 gtcacaaccg agataataag aacaagccgc atttcagtat ccagaaggat tgctccttga
      901 gtattaatca caggttcagg atgaagcgat ggatatagcc gcggaacaac aaatattaaa
      961 aatggtgaag cgacaaacgc tataacaaga tacacacttt caagcatagc accttttggg
     1021 ttagtatgca caatttgatg caggctaaaa tacgctatat ataccaatag caggagcgtg
     1081 attgacgttt cacgtggatc ccagttccag taacttcccc acataacctt tgcccacagt
     1141 gaacctgtta tcacggtcaa tatgctgaaa aaaaatccaa tgtgcactga acttcgtgca
     1201 caatgaatat actctattgc attctttcgt atgtactgta ttgaagcaag acctgcaaca
     1261 ataaatgcca gtgttgacac ccaggcaagt ggtacgtgca aatacactat cctgcttgtt
     1321 tccccaagga ttgctgcagg cggcacccat atatatatga gatacagcgt tgccagcatt
     1381 aacagtgcat caattatgat taaaaccatg aaaaactcca tgagaaatta agattcacct
     1441 aaaattctct ccatatatag gggaacagta tatatgatac cacgccaagc aatgttgaaa
     1501 atgcaagtag aaaaagtata gcactccatg aagaataaat ctctttagaa aaacactgtg
     1561 ttgtagattt tattacgact gtgataatgg gtagcgttat ggggaacgat aaaatagtaa
     1621 aaaggccgcc tcgcgcctgt accagtgcgg ttatagccgc gattatggtt gtgcacactg
     1681 ccagagcact actgcctgcc agcaccatga ttgcaaaacc tgatacatta tacactgaaa
     1741 catccataac aaacagatac agcaatccag ctattgctgc aacagccacc ataataatac
     1801 aatttacaat aagctttgaa acaaatatgc cttcagggct ataatacatc aggttaaaga
     1861 gtgcagtatg ttcttctatc tctctgtaaa agaggtgaga ggtattcatc cctgaaaaaa
     1921 atataatgac ccacaaaaga atagcatgca ccattgggct cccaattatg ccacctgaag
     1981 caaaaccaat agaaattgtc acaatgacac tgaacgatag tatcagcgaa atgactacct
     2041 tgcttctgaa ctctatacga agatctttta caatattacg caccaatccc tttatcaagc
     2101 acaatactcc tgtgacaaag ttttgcttcc ctgctgtcat tagttgccat gataatagca
     2161 gtaaatggtt gtatttcttt tattagttca aaaagaattt ttcttccact atcatctaaa
     2221 tttgaacctg gttcatccag caataaaact tttggttttt tcaaaagtgc cattgcaatc
     2281 ttgcaacgtt gctgcatgcc tgtagaatag gtatggagaa ctttatcttt atgctgatac
     2341 atatctaaac gatgtagaag ttctatagca tcatcagtta ccgagccaaa ggcaaagata
     2401 atattttctg ttaatgtaag atgattgtaa cactgtatgt agggagcaac aatgccagca
     2461 tagtggataa ccgtatgggc agggatggat tccatcttat caaaatactc taccctgcca
     2521 ctatcaggtg acacaatatt tcccagtatt ttcagcaagg ttgatttgcc actgccattg
     2581 ggaccaacaa ttgcaacaga ttcacctgtt gatactgaac atgaaatatt gctgaacaac
     2641 ctgtagctat agcgttttga tatattgtga gcttttattg taaacacaaa accttacaga
     2701 actatttctt ttttagctgt tgccatttca ataacaggta attttactgc cttatctttc
     2761 ataacctcat ccactgtctt ctgatcagct aatcggtaca gtaatcttac ctggatgcta
     2821 tattgccctt ttaatccttt gggcagtatg atagtattgg tagccgattc ttttggtgaa
     2881 atacgattat caaacagaat ttctttagct tttgcaatgt tgacaacagg cttccctttc
     2941 ccatcaccaa aaatagtctt aaagattata gtattatcag ggagatagcc ccttttatca
     3001 gccttcccaa ttgaaaaaac ctctttgcca tattgattca ggataactat ctccaaccac
     3061 atttgccg
//
LOCUS       DAOSZP010000243         3065 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_25091_length_3065_cov_3.697342, whole genome shotgun sequence.
ACCESSION   DAOSZP010000243 DAOSZP010000000
VERSION     DAOSZP010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3065
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25091_length_3065_cov_3.697342"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..713)
                     /locus_tag="PK348_06170"
     CDS             complement(<1..713)
                     /locus_tag="PK348_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008672001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HXK65834.1"
                     /translation="MSDSNYKAVFRCIAGCNEEYPLDEVVYRCKKCGELLEVHHDIDR
                     LKELSPKQWRELFDSRVGTTKWPYGSGVWSKKEWVCPEVNNENIVSMFEGNTNLFWAK
                     RFGAMIGMDDLWVKMCGNSHTGSFKDLGMTVLVSMVNEMIKRGKPIKAVACASTGDTS
                     AALAAYCAYAGIPSIVFLPRNKISTAQLVQPMSNGSIVMSLDTNFDGCMKIVQEITKD
                     NSIYLANSMNSLRIEGQKS"
     gene            complement(716..1993)
                     /locus_tag="PK348_06175"
     CDS             complement(716..1993)
                     /locus_tag="PK348_06175"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004976.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HXK65835.1"
                     /translation="MKIRCGLIGFGTVGSGVYTLLQKHRTDIYKKTGVDIELVTICDV
                     RDIKDKVNVPVTGSWQDVVNDASIDVVIELIGGIEPAKTIILSALNAGKSVVTANKKL
                     LAESGNDIFTASQKAKGALAFEAAIGGGIPCVAALRNGLVGNAIQSVVGILNGTTNYI
                     LTRMAEDGMSFDEALKLAQAKGYAEADPTFDIEGFDAAHKIALLSMLAYNVRVSMQNI
                     YIEGITKITSLDIKYAHEMGYTIKLLGVSRMVDDKLDIRLQPVMIPFTHPLASVRNEF
                     NAVMYNGDMTGAVTLYGKGAGSLPTASAVVSDVVSIAQKQNGIKDSVYIANDAQYLSS
                     DDRMLRYYVRLHTEDRAGILSKISGVFASYNISIASVIQKEVNAPYVPLVITTHAARE
                     AAMHKAKQDIEQFNFVNGQVMVIPIEDFNSTGE"
     gene            complement(2291..2689)
                     /locus_tag="PK348_06180"
     CDS             complement(2291..2689)
                     /locus_tag="PK348_06180"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HXK65836.1"
                     /translation="MNYLLDTHVFLWTLFNDELLSKNAVSIISNPENTVLVSVISFWE
                     ISLKYNAGKLSLEGVLPDELPACAEKAGLEIMNVTPAEVSSFYKLPILKHKDPFDRLL
                     VWQCINNNICLISKDSDLAEYKNYGLQIMW"
     gene            complement(2686..2925)
                     /locus_tag="PK348_06185"
     CDS             complement(2686..2925)
                     /locus_tag="PK348_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020610880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HXK65837.1"
                     /translation="MKVFQVGELKSKFSNVLDYIRKGEEITISYGKKKEKIAVIIPYS
                     KYKKAKPRKLGILKNKASFKIHDDFMISDEELLHL"
BASE COUNT          928 a          697 c          471 g          969 t
ORIGIN      
        1 attgacttct ggccttcaat cctgagtgag ttcattgaat ttgccagata gatactgtta
       61 tcctttgtta tttcctgtac tatcttcata cagccatcga agttagtatc aagtgacatg
      121 acaatggaac cattgctcat gggctgcact aactgtgcag tagaaatttt gttgcgcggc
      181 aaaaaaacaa tagatgggat acccgcatac gcgcagtagg ctgccagcgc cgctgatgta
      241 tcacccgttg atgcacaggc aaccgcttta atgggcttac cccgctttat catttcattc
      301 accatggaca ccagtactgt catccctaaa tccttgaatg aaccagtatg actgttaccg
      361 cacatcttta cccacaggtc gtccatccca atcattgctc caaaacgttt tgcccagaat
      421 aaattggtat tgccttcaaa cattgacaca atattttcat tattaacttc agggcacacc
      481 cattccttct tgctccacac tccgcttcca tacggccact ttgtggttcc tacacggctg
      541 tcaaaaagct ctcgccattg tttgggcgat agttccttta atcggtctat gtcgtgatgc
      601 acttctaaca gttcaccaca ctttttacac ctgtagacaa cctcatcaag gggatattct
      661 tcattacaac ctgcaataca tcgaaaaact gctttatagt tactatcgct cataattatt
      721 cacctgtact attaaaatct tcaataggaa tgaccataac ctgaccattg acaaaattaa
      781 actgttcaat atcctgtttg gctttatgca ttgcagcttc acgtgctgca tgcgtggtaa
      841 tgacaagcgg aacatacggc gcattaacct ctttctgtat gacagaagca atactgatgt
      901 tatatgaagc aaacacgcca gaaatctttg acaaaatacc tgccctatct tcagtatgca
      961 accgtacata atagcgcagc attctgtcat ccgatgaaag gtactgcgca tcgtttgcta
     1021 tatacacact gtcttttatg ccattctgtt tttgtgcaat ggaaacaaca tcactaacca
     1081 ctgctgatgc tgtgggcaaa ctgcctgctc ccttgccata cagggtcact gcgccagtca
     1141 tatcgccatt atacataacc gcgttaaatt cattgcgcac tgaagccagc ggatgggtaa
     1201 acggaatcat caccggctgc aggcggatat caagcttatc atctaccatg cggctgaccc
     1261 ctaaaagctt gatggtatag cccatttcat gggcatactt tatatccaaa cttgtaattt
     1321 ttgtaatgcc ttctatataa atattctgca ttgaaacacg aacattatat gccagcattg
     1381 acaatagcgc aatcttgtgt gctgcatcaa agccttcaat atcaaaggtt gggtcagcct
     1441 cagcatagcc ttttgcctgt gccaatttca atgcttcatc aaacgacatg ccatcctcag
     1501 ccatccgtgt taaaatgtag ttggtggtac cgttaaggat accaactacc gattgtatgg
     1561 cattgccaac aagcccgttt cgcaacgcag caacacacgg aattccacca ccaatggctg
     1621 cttcaaacgc cagtgcccct tttgctttct gtgaagcggt aaaaatatca ttcccgcttt
     1681 cagctaaaag ctttttattt gctgttacca cactttttcc cgcattaagt gcagaaagaa
     1741 taattgtctt tgcaggttct atcccaccaa taagctcaat gacaacatca atagaagcgt
     1801 cattaacaac atcctgccag ctacctgtaa ccggcacatt aaccttatct tttatatcac
     1861 gtacatcaca tattgtgaca agctcaatat ccacacccgt ttttttatat atatctgttc
     1921 tgtgcttttg caacagcgtg tacacaccgc ttcccacagt accaaaacca ataagcccac
     1981 atcgtatttt catagcatac ctcgaatata aaaaatatcc atcttgccct caatcattct
     2041 gattttacct atacacactt tctttaatat atggtcaatg aattaaaaaa tacgcaatac
     2101 aaccttttgt cgaattggcc aacaacggtt ttcatatgca caaagataaa agtttttcat
     2161 tacagtatgt acatcacatt catacgatga tacaggtttc gttgtcaact gtaattatat
     2221 atgtattttt ttaagtttta aagtcatcta aacaggaatt ccatcgcaca atggtaataa
     2281 cttatcccta ttaccacatg atttgtaatc catagttttt gtattcagct aaatctgaat
     2341 ctttggaaat caaacagata ttattattga tacactgcca caccagtaac ctatcaaatg
     2401 gatctttatg ttttagtatt ggtagtttgt aaaaagaaga aacctcagcg ggtgttacat
     2461 tcattatttc caaaccagcc ttttcggcac atgcaggcaa ctcatcaggt aaaactcctt
     2521 ctagtgataa tttgcctgca ttgtatttta acgaaatttc ccaaaaactg attacactta
     2581 ctaaaacagt attttcagga tttgaaataa ttgaaacagc attttttgat aataattcat
     2641 cgttaaacaa tgtccacaaa aaaacatggg tatcaagcag gtaattcata aatgtaacaa
     2701 ttcttcatca gaaatcataa aatcatcatg aattttaaaa gaagctttat tttttaaaat
     2761 acccaacttt cttggtttag cttttttata ttttgaatac ggaattataa cagcaatttt
     2821 ctctttcttt ttcccatagg agatagtaat ctcctcgcct tttctaatgt aatcaagtac
     2881 attagaaaat tttgacttta attcaccaac ctgaaatacc ttcataacag acaagatact
     2941 ataaagttgt caagttgtca actttaatta tgcaatattt caaaatcaat tgcttgactt
     3001 ttgaccacca gtatttttag attaaactgt ttcatatgtg tgaatatata taattatata
     3061 ttatg
//
LOCUS       DAOSZP010000244         3064 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_25101_length_3064_cov_3.631107, whole genome shotgun sequence.
ACCESSION   DAOSZP010000244 DAOSZP010000000
VERSION     DAOSZP010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3064
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25101_length_3064_cov_3.631107"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..676
                     /locus_tag="PK348_06190"
     CDS             <1..676
                     /locus_tag="PK348_06190"
                     /inference="COORDINATES: protein motif:HMM:NF012803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HXK65838.1"
                     /translation="INTAIRTQTGGYQGISFAIPASIVKQVVGELIQYGRVRRGWLGF
                     LVQEKFIKDGAQTQVEVISVVDGSPASRAGIEVGDIVRRVDGVQIKKISDLVKSIHAK
                     QVGSPIELVIARDGQLLTYNFVLHEKTTAKKGNQWLTALKSRYGITIDDARNGVVIVE
                     VLQFGLAYNVLKKGDIIQSIDGMGIASLQDVIAVLKKNNGVIQNCIVLRGGRAIRCSF
                     NSQEVE"
     gene            679..1113
                     /locus_tag="PK348_06195"
     CDS             679..1113
                     /locus_tag="PK348_06195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65839.1"
                     /translation="MKWIYSTILFLLCAIVTVHVTADEAKEVVLDTQWYTSLQNSDAI
                     KRLQMLDALKDKVVKAQGQVIKVEESNLLKKKYRIILSVNLPKIKILYYCYTDKEDYS
                     DLLQPQDTFEFSGQFVVATPLNSRIDSILCDIILEDGAVVVK"
     gene            complement(1118..1735)
                     /locus_tag="PK348_06200"
     CDS             complement(1118..1735)
                     /locus_tag="PK348_06200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65840.1"
                     /translation="MKKITRTNDIKTNTPIRVYKKGYGYTLLTLIENNDLFLICKSPE
                     DFFTYIPENETVECYIWPYPDGSYDFTTTVLGKISSPFPIILLKHTDSVVYNPTRQCL
                     RAKVNIPFTFFTFSINTTKSFSSEDIQWHQGTILELTDREAVLETAMKPEHFIKGHLH
                     LDTKDIDITGKVTGINSTHYDISFVGLDDADRIAILDYIFTVYRE"
     gene            2086..2379
                     /gene="rpsF"
                     /locus_tag="PK348_06205"
     CDS             2086..2379
                     /gene="rpsF"
                     /locus_tag="PK348_06205"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HXK65841.1"
                     /translation="MNKYELIVILRPSDKLESLKEKVREILKKNQVVIESDEPWGIRS
                     LAYTIDRANDGYYEYFIAQINPTAVSNIIAEFRLQPDILRYLFVKLPTLSKTA"
     gene            2385..2807
                     /gene="ssb"
                     /locus_tag="PK348_06210"
     CDS             2385..2807
                     /gene="ssb"
                     /locus_tag="PK348_06210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HXK65842.1"
                     /translation="MAGDLNKVILVGRVVRDPELRYTPGGSAVVSFSVANNRIYVKNN
                     EKQEVVSFFNCVAWGKLAETVANYCKKGQQLALEGRLQQRSWQDKEGNKRSTVEIVAE
                     SLQFLGGRQQSDSQVTIEQDEPVDINSDQPFADDEIPF"
     gene            2839..>3064
                     /locus_tag="PK348_06215"
     CDS             2839..>3064
                     /locus_tag="PK348_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017808746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65843.1"
                     /translation="MSEEIKHDETIEAQDNVNPQEVKPEAVAPEEDDKVLATAVQVQR
                     IPKFKKKVCRFCEDPNVAIDYKNPDLLVRFI"
BASE COUNT          985 a          480 c          671 g          928 t
ORIGIN      
        1 catcaataca gcaatacgga cgcagacggg tgggtatcag gggataagtt ttgcaattcc
       61 tgcttctatt gttaaacagg ttgttggtga actcatacag tatggcaggg tgcgccgtgg
      121 ctggcttgga tttttagttc aggaaaaatt tataaaagat ggcgcacaaa cgcaggttga
      181 agttatatcg gttgttgatg gttcgcctgc ttcccgtgca ggtatagaag ttggtgatat
      241 tgtacgcagg gttgatggtg tacagataaa aaaaatttca gacctggtaa aatcaattca
      301 tgctaaacag gtgggctcac ctattgaact ggttattgct cgtgacggac agctattgac
      361 atataatttt gtgttacatg aaaaaacaac tgctaaaaaa ggtaatcaat ggctcactgc
      421 tttgaagtcc aggtatggaa tcacaattga tgatgcacgc aatggtgtgg ttattgtcga
      481 agttcttcaa tttggtcttg cttacaatgt attaaaaaag ggcgatatca tacagtctat
      541 tgatggtatg ggtattgcat ctttacagga tgtaattgca gtattgaaaa aaaataatgg
      601 agtaatccaa aattgtattg tactgcgagg cggcagggct atacgatgtt cttttaattc
      661 acaggaagtt gagtaattat gaagtggata tatagtacca ttttattttt attatgtgcg
      721 atagtaactg tccatgtaac tgctgatgaa gcaaaagaag ttgtactgga cactcaatgg
      781 tatacttctt tacaaaacag tgatgcaatc aaacgattac agatgcttga cgcattgaag
      841 gataaagtgg ttaaagctca gggccaggtt atcaaagtag aagaatctaa tcttttaaaa
      901 aagaaatata gaattatcct gtctgtcaat cttccaaaga taaagattct gtattattgc
      961 tataccgata aagaagatta ttcagatctt ttacaaccac aggacacctt tgagtttagc
     1021 ggccagtttg ttgttgcaac accactcaat tctcgaatag attccatact atgtgatatc
     1081 attctggaag atggtgctgt ggtggtaaaa tagcggatta ttcccggtaa acggtgaata
     1141 tataatccaa tattgcaatc ctatcagcgt catccagccc tacaaaagaa atatcataat
     1201 gagtgctgtt gatacccgtt actttgccgg ttatatctat atcttttgta tctaagtgaa
     1261 ggtggccttt aataaaatgt tccggtttca ttgctgtttc aagtactgct tccctgtcag
     1321 taagctcaag aatagtaccc tggtgccatt gtatatcctc agaagaaaag ctttttgtgg
     1381 tattaattga aaatgtaaag aaggtaaagg gaatatttac tttagctctg agacactgac
     1441 gtgtggggtt atagacaacg gaatcggtat gtttaagtaa aatgatggga aatggtgaag
     1501 aaattttacc cagcactgta gtggtgaaat catacgaacc atcaggatat ggccatatat
     1561 aacattccac agtttcattt tccgggatat atgtaaaaaa atcctcaggt gatttacata
     1621 taaggaataa gtcattattt tcaattaatg taagcaatgt atatccatac ccttttttat
     1681 acacccggat aggggtattg gttttaatgt catttgttct ggtaattttt ttcatgatac
     1741 aattttctcc agaaagtcag tcaatacatg gtgtcattac aggcaatttt tgcctaaaaa
     1801 accagtgaaa taatataaaa taaagcattt caagtcaagg gcaaattgtt aacgtcaggt
     1861 aaaattatta taattaatgt attcatccat ttaatagaac cctggaggga ttaaatttag
     1921 gataccattg tggtgaattc aaaaaattag tttttaaaaa tttacagttc ccacaaaaaa
     1981 gtattgaaaa aaagttgttg caaattgtat tatgtgcaaa aatagtggta gtccttctgc
     2041 tgcacgcgta tgcagtgcag ctactattat ccataggggg taacaatgaa taaatacgaa
     2101 ttaatagtca tattgcggcc ttcggataag ctagaatcgc ttaaagaaaa ggtccgtgaa
     2161 attttaaaga aaaatcaggt tgttattgaa tctgatgaac catggggtat acggagttta
     2221 gcctatacta tcgacagggc aaatgacggt tactatgaat attttattgc ccagataaat
     2281 cccacagcgg tgagcaatat tattgccgaa ttcagattgc agccggatat cctccgatat
     2341 ctttttgtta aacttccaac actaagcaaa acagcgtgac gtgtatggcc ggtgatttaa
     2401 acaaggttat actggtagga agagttgtca gggatcctga gttgcgatac actccgggtg
     2461 ggtcagctgt tgtttccttt tcagtggcca ataacaggat ttatgtaaag aataacgaaa
     2521 agcaggaagt tgtttcgttc tttaattgcg ttgcatgggg caagctggct gaaactgttg
     2581 ccaattattg taaaaagggg cagcaactgg cattggaagg aagactgcag cagcgttcct
     2641 ggcaggataa agaaggaaac aaacgttcaa cggtggaaat agttgctgaa tcgttacagt
     2701 ttttgggtgg caggcagcag tctgattcac aggttactat cgaacaagat gagcccgttg
     2761 atataaatag cgatcagcca tttgctgatg atgagatacc attctaacta ttcacattaa
     2821 ggtataagga gtactataat gagtgaagaa attaaacatg atgaaactat agaggcgcag
     2881 gacaatgtta atcctcagga agttaagccg gaagcggtag ctcctgaaga agatgataag
     2941 gtgctggcaa ctgctgttca ggtgcagcga atacctaaat ttaagaaaaa agtatgtcgt
     3001 ttctgcgaag atcctaatgt agctattgat tataaaaatc cggatttact ggtcaggttt
     3061 atta
//
LOCUS       DAOSZP010000245         3063 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_25115_length_3063_cov_3.215758, whole genome shotgun sequence.
ACCESSION   DAOSZP010000245 DAOSZP010000000
VERSION     DAOSZP010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3063
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25115_length_3063_cov_3.215758"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(132..1169)
                     /locus_tag="PK348_06220"
     CDS             complement(132..1169)
                     /locus_tag="PK348_06220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65844.1"
                     /translation="MHKLHYIYIIVLLWLPCRLQAEYYPFILNAKPVICHQCKHFDNS
                     LYQSLIYTLTASGIFIGDSSSYSIPAGAYNPPGNNQLLRNLSIDITYSRKIHYKELTT
                     YLKEKVETESYTIAITIASSENPQEFTALYPNISPHQLEHTFNSIAQDILQFYSSKRI
                     PVLYQSPFAFYVTGLTLAPVYSILHDKLNNYAHSATGFQCLFTINNTYIPLTIMPSIT
                     WFTLNNPIKTINSWHSVSFMMHAGYQQALPFSVSITPYIGAGYILHYIEGDKTHTYPP
                     YSYSHSFYYNPQVATGIIFELILDPQLSLEAGAAYNMFFASSSQFSYLQYSFGFTFHG
                     NILLYQATPYH"
     gene            1346..2788
                     /locus_tag="PK348_06225"
     CDS             1346..2788
                     /locus_tag="PK348_06225"
                     /inference="COORDINATES: protein motif:HMM:NF025670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65845.1"
                     /translation="MKHIVVFFMLSCAAIIYLPGCSDINYPTDALDKDPNDMSGGSIV
                     IDGGAQFAYSQQVTVTCNVDNAIQMQFSTDATNWSTREAFNKARVLELPLLYGQQSVY
                     GQFFNSRGDAVMYDDSIFFIERCIASTSAYLGGDVAISDDGTVIAAGSYEGNANAVYV
                     FRKQTIDWDCTVIKPDDISTGDKFGYSVALSGDGTWLFVGAPVKRAVYVYEYTGGWQQ
                     QQKISSSTLSFGYDVATDTIGLDLGVVSYDGKTFEIYKRNDVKQYTLKHQIPYSKAKS
                     YCVKIKNQSDYAYVFLGLNSDNKGRVEVFKLTSDGIGGINTITPADNQEYADFGKSIS
                     ITSDGSVCVIGAPYYDINADDDKGCFYVYQKSQSSYTLVSQVKNISGTNGDALGMAVS
                     ILPDGQYMFASMPLADEGGVDRGRVLVYRLNGSNVEYMDSFYPDDRNEKCYFGYSLAV
                     SSTGVVAIGAPYAFTHATNDNGGVVYIRRL"
     gene            2882..>3063
                     /locus_tag="PK348_06230"
     CDS             2882..>3063
                     /locus_tag="PK348_06230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65846.1"
                     /translation="MVQHVIKAIIALITVLMTTTMAVAQLSVSQPEVEIGHTVATSRF
                     IGMMGGMYGYAYPAGS"
BASE COUNT          978 a          426 c          712 g          947 t
ORIGIN      
        1 aaagtacctg aaatacgccc cttacgtaaa ggcgatgagt ttaaggatgt gtttgatacc
       61 tttactaaaa tggttgaatc attacattta aaacacagga aaaattttaa acaaaagaag
      121 taaatacaaa gttaatggta tggagttgcc tgatacagta atatattgcc atggaatgta
      181 aaaccaaaag agtattgaag ataagaaaac tgtgatgagg aggcaaagaa catattgtat
      241 gcagcacctg cttctagcga tagttgtgga tcaagaataa gttcaaaaat aatgcctgtt
      301 gctacctgag gattataata gaatgaatgt gagtatgaat atggcggata ggtatgagtt
      361 ttatcgcctt ctatgtaatg caaaatataa cctgctccaa tgtagggggt aatgctcact
      421 gagaatggta aagcttgctg gtaaccggca tgcatcataa aagaaacgct atgccagcta
      481 ttaatggttt taatgggatt gtttaatgta aaccaggtaa tagaaggcat tatagttaga
      541 gggatgtatg tgttattaat tgtgaataaa cactgaaacc cggttgcaga gtgtgcgtaa
      601 ttgtttaatt tatcatgcag gatgctgtaa acaggtgcca gggttaatcc ggtgacataa
      661 aaagcaaacg ggctttgata gagtacagga atacgttttg atgaataaaa ctgtagtata
      721 tcctgagcaa tagaattaaa tgtgtgttcc agttgatgcg gtgaaatgtt gggataaagg
      781 gctgtaaatt cctgtggatt ttcagatgat gcaatggtta tggcaattgt gtaactttca
      841 gtttctactt tttctttaag gtatgttgtg agctctttgt aatgtatctt acgggaatag
      901 gttatatcta tagaaagatt gcgcaacagt tgattgttac caggtgggtt atatgcgccg
      961 gcagggattg aatatgatga actatcgcca ataaaaatgc ctgatgctgt caatgtataa
     1021 ataagtgact gatagagtga attgtcaaaa tgtttacact gatggcagat aacaggcttt
     1081 gcgttcagta tgaatggata gtattcggcc tgcagcctgc agggaagcca tagaagcact
     1141 atgatatata tgtaatggag tttatgcatg acagttaaac attatgtata tgacaataaa
     1201 tacaaagtaa attatttaat caagaatatt tttataatta ttttatttat tatgcttatt
     1261 attatttgct cattatattt actgcaatat atattgctta aaatgagttg ttttatcaac
     1321 tgatttcatt gttagggtta ctaaaatgaa acacatagta gtatttttta tgctatcatg
     1381 tgctgcaatc atttacttac cgggatgcag cgatattaat tatcctaccg atgcactgga
     1441 taaagatccc aatgacatga gtggtggcag tattgttatt gatggtggag cgcagtttgc
     1501 gtattcgcaa caggttactg ttacatgcaa tgtggataat gctattcaga tgcagttttc
     1561 aacagatgcc acaaactgga gcacacggga agcatttaac aaagccaggg tactggagct
     1621 tccgctactg tacgggcaac aatcagtata cgggcagttt tttaacagcc gtggtgatgc
     1681 ggttatgtat gatgattcta tatttttcat tgagcgctgt atagcaagta cctctgcata
     1741 ccttggtggt gatgtggcta tttcggatga tggtacagtt attgctgctg gaagctatga
     1801 aggcaatgcc aatgcggtat acgtattcag aaaacagaca attgattggg actgtacggt
     1861 gattaaacct gatgacatta gcacaggtga taaatttgga tatagcgttg ccttgagtgg
     1921 tgatggtaca tggctttttg taggtgcacc ggttaaaagg gcagtgtatg tgtatgagta
     1981 cacaggcgga tggcaacaac aacagaaaat ttcatcatcc actttatcgt ttggttatga
     2041 tgtagctaca gatactattg gattggattt aggagttgtg tcgtatgatg gaaaaacatt
     2101 tgagatatat aaaagaaatg atgtaaaaca atatactcta aaacatcaaa ttccttattc
     2161 taaagcgaag tcttattgtg tgaaaattaa gaatcaatct gattatgcat atgtatttct
     2221 tggtttaaat tcggataata aaggtagggt agaagtattt aaacttacat cggatggaat
     2281 agggggtatt aatactatta ctcctgccga caaccaggag tatgctgatt ttggcaaatc
     2341 aataagtatt accagcgatg gcagtgtatg cgtcattggt gcaccatact atgatataaa
     2401 tgcagatgat gataaaggat gcttttatgt atatcaaaaa agtcagagca gctatacatt
     2461 agtttcacag gttaaaaata ttagtggaac aaatggtgat gcactgggga tggcggtaag
     2521 tattttgcca gatgggcagt atatgtttgc atctatgcct cttgctgatg aaggtggggt
     2581 tgatagaggc agggttttag tgtatagact caatggatca aatgttgaat atatggattc
     2641 tttttatcct gatgacagaa atgaaaagtg ttattttggc tattcacttg cagtaagcag
     2701 taccggtgtt gttgccattg gggcaccgta tgcatttaca catgcaacca atgataatgg
     2761 gggagtggta tatatacgca ggttgtaatg atggtgtttg ttgtaatcat tttaaaccca
     2821 acagggcggg aagtaaaaat gacaatacaa tagaaatctt tgctaaaagg gaaccgtggt
     2881 aatggtacag catgtaataa aagcaataat agcactaata acagtattga tgacaaccac
     2941 aatggcagta gcacagcttt cagtatcgca accggaggtt gaaatcggtc atactgtggc
     3001 taccagtaga tttattggaa tgatgggagg gatgtacggc tatgcatatc ctgctggctc
     3061 aat
//
LOCUS       DAOSZP010000246         3061 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_25139_length_3061_cov_2.660013, whole genome shotgun sequence.
ACCESSION   DAOSZP010000246 DAOSZP010000000
VERSION     DAOSZP010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3061
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25139_length_3061_cov_2.660013"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..463
                     /locus_tag="PK348_06235"
     CDS             <1..463
                     /locus_tag="PK348_06235"
                     /inference="COORDINATES: protein motif:HMM:NF016314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF501 domain-containing protein"
                     /protein_id="HXK65847.1"
                     /translation="KCNFGFPQIILLSPIVNDVIDYQSLSNILWLTCPYLNDAIHQLE
                     NKGYIIKIENLINSDNQLKQEMAKAHAHFYFFRKSLYEKYFGTDINDDYLNILKRGVG
                     GLQDTIHIKCLHLHYAHYRICESNVAGRIVYHLLQQKVNCDDAICRVSLQK"
     gene            429..1097
                     /locus_tag="PK348_06240"
     CDS             429..1097
                     /locus_tag="PK348_06240"
                     /inference="COORDINATES: protein motif:HMM:NF013468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HXK65848.1"
                     /translation="MMQFVESPYKNDDVIVIRKLKKDGSVDTEIIQRITEILHKNGLV
                     ILPVDSQYEIVGIANAAVEHKLKTIIKSRTKKFVRLIASFKMLESLATVTKFQYDFLH
                     RIWPGEVTAIVPRKEDISQEIALRFPRTKFVQEIIEAVGQPLFATNILRVTKGSNKHS
                     DIIRVFGKKVDAIVIIEELCKRHPKPTLISLFNGKLKILRPGKISSEEIQSYYYLGSC
                     DEEM"
     gene            complement(1098..1919)
                     /locus_tag="PK348_06245"
     CDS             complement(1098..1919)
                     /locus_tag="PK348_06245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65849.1"
                     /translation="MDTKSTHSEQLAYRAYVENNIQYINEIHKKIEKLILKLEKEHIS
                     IKECFTQLENEINLLLKVYSHFTSLGGYNYCLKLKALIDSYQKVTNTEIQLSALYYIH
                     SKILELQEEQLKHFPELTQKKALYDKLTSTKKFVEDDVHNCTYKWITFKRNAMWFVVL
                     FDSYQLLNTSDVSGLRPLSQNYYQATFNNTTITIRDPLRLDEQPSTTVVLVNYHNSIY
                     GYFYDSIGKKLGARSNILQQMAKAPEGYSLPLYGRIRLFGDSYIYLKHVDVNKPG"
     gene            complement(1926..>3061)
                     /locus_tag="PK348_06250"
     CDS             complement(1926..>3061)
                     /locus_tag="PK348_06250"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HXK65850.1"
                     /translation="LLHIAYKNQLPVHGTIKQTTNGGFIVSVGGIQAFCPLSQIDTKI
                     QESDSYLNKSFDFAIMRFEQQGKNIILSRRSLLDKIKEEAIEKLKQQLHSGDIVQATV
                     VQVLDAGLLVSLHSLDAFVPKSELSHSRKPNMNLYEPGQTITAKILDLDWNKVKITLS
                     IKQTLPEPWHYISQYQVGNTYTGTIAKIIKNGAFVEFDNGLEGFIPLSRMSYVKKLHD
                     PREVCNEGDSVQVKIIDIDQTSKKITLELITSEENPWLTVNESLRDSIQTATIEEITA
                     KGLVVVLPNQVRAFVPKEQCAIDRNADITKSYQVGATIKVVVTTVNADDRKCIASEKK
                     AQEIEERQSYEKFIASNTSQGSSAFGQLFKQKYEEMQKKSKNS"
BASE COUNT          916 a          549 c          579 g         1017 t
ORIGIN      
        1 aaaatgcaac tttggatttc ctcaaattat tcttttatca cctatagtta atgatgtaat
       61 agattatcaa tcgttgtcca atattctgtg gcttacatgt ccctatctca atgatgccat
      121 ccaccagctt gaaaataaag gatacataat aaaaattgaa aatttaatca attcagataa
      181 ccagttaaaa caggagatgg caaaagcaca tgctcatttc tactttttca gaaaaagtct
      241 ctatgaaaag tattttggta ctgacattaa tgacgactat ttaaatatat tgaaaagagg
      301 cgttggcgga ttgcaggata caatccatat aaaatgcttg catttacatt atgctcatta
      361 tagaatttgc gaaagtaatg ttgctggaag gattgtgtat catttattac agcaaaaggt
      421 aaattgcgat gatgcaattt gtagagtctc cttacaaaaa tgatgatgtc atagtaatac
      481 gaaaactcaa aaaagatggt agcgtagata ctgaaattat acaaagaatt accgaaattt
      541 tacataaaaa tggccttgtt atcctccctg ttgactcaca gtatgaaata gttggtattg
      601 caaatgcagc ggttgagcat aaattaaaaa caattattaa atcaagaaca aaaaaatttg
      661 tacgcctgat agcatcattc aaaatgctgg aatcccttgc aacggtcacc aagtttcaat
      721 atgattttct gcacaggata tggcctggcg aggttactgc aattgtgcca cgaaaggaag
      781 atatatcgca ggaaatagca ttgcgttttc ccaggacaaa gtttgttcag gaaataattg
      841 aagcagtggg gcagcctctc tttgccacaa atattctcag ggtaacaaaa gggtcaaata
      901 agcactctga tattattcgt gtatttggaa aaaaagttga tgccattgtt attatcgaag
      961 agttgtgcaa acgtcaccct aagccaacac tcataagcct ctttaatggt aaactcaaga
     1021 tattgcggcc agggaaaatc tcttccgagg aaattcagtc atactattat ttaggcagct
     1081 gtgatgagga aatgtaatta tccgggtttg tttacatcaa cgtgtttaag atatatataa
     1141 ctatcgccaa ataacctgat ccttccgtat aatggcaatg aatagccttc aggcgctttt
     1201 gccatttgct gcagaatgtt gctccgtgca cctaattttt ttccaatgga atcataaaag
     1261 taaccatata tgctgttgtg gtaattgaca agcactacag tggttgaagg ctgttcgtca
     1321 aggcgcaaag gatcacggat tgtaattgtg gtgttattaa atgttgcctg atagtaattc
     1381 tgtgaaagag gccttagacc tgatacatca ctggtattta gcaactgata actatcaaaa
     1441 agaacaacaa accacatggc attacgtttg aaggtaatcc atttgtaagt gcaattgtgt
     1501 acatcatctt ccacaaattt ttttgtggaa gtaagcttat catataaagc tttcttctga
     1561 gtcaactcag ggaaatgttt caattgctct tcctgtagtt caagaatctt ggaatgaatg
     1621 tagtacagcg cagatagctg aatctcagta ttggtaactt tttgatatga gtcaatgagg
     1681 gcttttaatt taaggcagta gttgtatcca cccaacgatg tgaaatgcga atacactttc
     1741 aatagcagat taatttcgtt ttcaagttgg gtaaaacatt ccttaattga gatgtgttcc
     1801 ttttcaagtt ttaaaataag cttttcaatc ttcttgtgga tttcgttaat atattgaata
     1861 ttgttttcta cgtatgctct atatgccagt tgctcactgt gtgtactttt agtatccata
     1921 caggtttatg agtttttgga ttttttctgc atttcctcat acttctgttt aaacagttgc
     1981 ccaaaggctg aagaaccctg actggtgtta gatgcaatga atttttcgta tgactggcgc
     2041 tcttcaattt cctgtgcttt tttttctgaa gcaatgcatt ttctgtcatc ggcattaact
     2101 gtagttacta caactttgat ggttgctccc acctgatatg actttgttat atcagcatta
     2161 cggtcaattg cacactgctc tttgggaaca aacgcacgca cctgatttgg cagcaccaca
     2221 acaaggcctt ttgctgtaat ttcttcaatg gttgcagttt gtatggaatc acgcagtgat
     2281 tcattgacgg taagccaggg attttcttcg ctggtgataa gttccagggt gattttttta
     2341 ctggtttggt ctatatctat aattttaacc tgtacactat cgccctcatt acatacttcc
     2401 ctggggtcat gtaacttttt gacatagctc atgcgtgaaa gtgggataaa cccttcaagg
     2461 ccattatcaa attcaacaaa tgcaccattt ttaattattt tggcgatagt tcctgtatag
     2521 gtatttccaa cctgatattg acttatataa tgccatggtt caggcagtgt ttgcttaatg
     2581 ctcaatgtta ttttgacttt attccagtct aaatcaagta tctttgcagt gatagtctgt
     2641 cctggttcat acagattcat gtttggctta cggctgtgcg atagttcact ttttggcaca
     2701 aaggcatcaa gtgaatgtag cgatactaaa aggcctgcat caagtacctg aacaacagta
     2761 gcctgaacta tatcgccact gtgtaattgc tgtttcagct tttctatggc ttcttctttg
     2821 attttatcaa gcagtgacct tcgcgacaga atgatgtttt taccctgctg ctcaaagcgc
     2881 ataatggcaa agtcaaagct tttattcagg taactatcgc tttcctgaat ctttgtatca
     2941 atttgcgata atggacaaaa tgcctgaata ccaccaacgg aaacaataaa tccgccattg
     3001 gttgtttgtt tgatagttcc atgtacaggt aactggtttt tgtacgctat atgcaaaaga
     3061 t
//
LOCUS       DAOSZP010000247         3060 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_25154_length_3060_cov_2.752745, whole genome shotgun sequence.
ACCESSION   DAOSZP010000247 DAOSZP010000000
VERSION     DAOSZP010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3060
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25154_length_3060_cov_2.752745"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..740
                     /locus_tag="PK348_06255"
     CDS             <1..740
                     /locus_tag="PK348_06255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HXK65851.1"
                     /translation="YLLYNPIQKMKVTMEGEKVIGLLHEVIAARGFEQETYFEMAIRD
                     IGMLPKLEGTTHVNMALVVKFLQNFMFKPKDYPEIPRRDDPANDSFLFNQGPTKGLGS
                     IQFHDYRKAYEGIRSKNLEVFMEQIEAFKNFLIKGTPDEKQTKNIDYMLTVGELFTLI
                     PYTQLICENKKIYNVEDEIIDEIFKFIVSDFSAYATRLYTGQKNTDKQNELILKMIKK
                     PVLDDENFNKVWKDHVYALKGQYTMNE"
     gene            925..2382
                     /locus_tag="PK348_06260"
     CDS             925..2382
                     /locus_tag="PK348_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968717.1"
                     /note="Members of the Solute:Sodium Symporter (SSS), TC
                     2.A.21 as described in tcdb.org, catalyze solute:Na+
                     symport. Known solutes for members of the family include
                     sugars, amino acids, nucleosides, inositols, vitamins,
                     urea or anions, depending on the system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/solute symporter"
                     /protein_id="HXK65852.1"
                     /translation="MWSISLLTIFLVLMVFIGVWGMKKTSTLGDFFLGGRTIGPWVSA
                     LAYGTSYFSAVLFIGFAGKLGWVFGLDAIWIGVGNALFGSFIAWLVLGRRTRIMTQNL
                     DVMTMPEFLMERFNGTYIKMFAAIIIFIFLLPYSASVFKGLGHLFEVNFHISYDLALI
                     IMIGITGIYLVLGGYFAITLTDFIQGIIMIAGAITMVLVLSAKSGGIAQSIALVAQKY
                     PLHIPADKHPGYLLLGALIFMTSFGVWGMPQMVQKFYAIKSEDLIKKASIVTGIFATI
                     IGISAYYTGSLSHIFYDAVPAIDGKPAFDRLIPDLLTQNLPEPLMAVILLLILSASMS
                     TLSSLVLVSSSAIAIDLYKGHVNPQISKENSVAMMRFLSALFIIISYFIARYEFAIII
                     TLMSLSWGAVAGAFMAPYLYGLFWKRTTLAGVYAGMITGLVTSIGLFFYLGANLSPIA
                     SSAGMLAPFVVVPLVSLFTKKPSDETITKAFEKIG"
     gene            2437..>3060
                     /locus_tag="PK348_06265"
     CDS             2437..>3060
                     /locus_tag="PK348_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008514552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXK65853.1"
                     /translation="MDFIPLENITMELADVAMGRRKADMVIKNGTLVNVNTKELQEHI
                     DVALYKGRIALVGDAKHCIGESTEIVDATGLFIAPGFMDGHIHVESSMLTVSQYSNAV
                     VPHGTTAIFMDPHEIANVLGLKGVRLMLDEAKDVPLRVYATVPSCVPSAPGLEDAGAV
                     IGASDIEQAMQWEGIAGLGELMNFPGVFNSVPEVHKEIEVTLKAGKPV"
BASE COUNT          912 a          573 c          645 g          930 t
ORIGIN      
        1 ggtatttact atacaaccct atccagaaaa tgaaagttac catggaaggc gaaaaggtaa
       61 ttgggctgct acatgaggtt attgcagcac gcggctttga acaggaaacc tactttgaga
      121 tggcaatccg cgatattggc atgttgccta aattagaggg taccactcac gtcaacatgg
      181 cgctggttgt aaaattttta caaaacttca tgttcaaacc aaaagactat cctgaaatac
      241 cacgacgtga tgacccagcc aatgacagct tccttttcaa tcaaggacca accaaaggat
      301 tgggttcaat ccagttccat gattaccgca aagcgtatga aggtatcagg agcaaaaatt
      361 tagaagtatt tatggaacag atagaagcct ttaaaaattt ccttattaaa ggaacacctg
      421 atgaaaagca aaccaagaat attgactaca tgctgacagt tggcgagctt ttcacgctta
      481 ttccttacac acagcttatc tgtgaaaaca agaagattta caatgttgaa gatgagatta
      541 ttgatgaaat cttcaaattc attgtaagtg atttttcagc atacgcaaca cgtctttaca
      601 caggccagaa gaacaccgat aaacagaatg agcttatatt aaagatgatc aaaaaacctg
      661 ttcttgatga tgaaaacttc aacaaagtgt ggaaggacca cgtgtatgca ttaaaaggcc
      721 agtacacaat gaatgaataa tgaaagaaac aaaaggcgcc ctaaaaaagg cgccttttgt
      781 tttactaccg ccagattttt tatcattgac atctaattgc aagatataaa aatacttaca
      841 ttgtttctgc tataatacta tagcagtttt atcatatcat attgcatgta acaccattct
      901 atatataaaa aaggagaact ggcaatgtgg tcaatttctt tacttacaat cttcttagtg
      961 ttaatggttt ttattggagt ctggggcatg aaaaagactt ccacacttgg tgatttcttt
     1021 ttaggtggaa gaacaattgg cccatgggtg tctgctctgg cgtatggaac gtcatatttt
     1081 tcagcagtgc tgtttattgg ttttgccggt aaactgggct gggtttttgg acttgatgca
     1141 atatggattg gtgttgggaa cgctcttttt ggttcattta ttgcatggtt ggttcttggc
     1201 aggcgtaccc gtatcatgac acaaaattta gatgtcatga caatgcctga atttttgatg
     1261 gaacgtttca atggtacata catcaagatg tttgcagcaa ttataatttt tatattttta
     1321 cttccctatt ctgcatcagt ttttaaagga ttgggacatc tctttgaagt taatttccat
     1381 atttcgtatg acctggcatt aatcatcatg ataggaatta ccggtattta cctggttttg
     1441 ggtggatatt ttgctattac attgactgat tttattcagg gtatcatcat gattgccgga
     1501 gctatcacca tggttttagt actttcagca aaaagtggtg ggatagccca aagtattgca
     1561 ctggttgcac aaaaatatcc attgcacata ccagccgata agcatcctgg atatcttttg
     1621 cttggtgctc tcattttcat gacgtcgttt ggagtctggg gaatgccaca aatggtacag
     1681 aaattttacg ccattaaaag tgaagattta ataaaaaaag caagcatagt aacggggatt
     1741 tttgctacca ttattggcat cagcgcctac tataccgggt cactatcgca catattttat
     1801 gatgctgttc cagctattga tggcaaacca gcatttgaca ggcttatccc cgacctgtta
     1861 acacaaaacc tccccgaacc actcatggcg gttatactcc tgttaatatt atcagcatcc
     1921 atgtcaacac tttcatcact ggtgctggta tcgtcgtcgg ctatagccat tgacctgtat
     1981 aaagggcatg ttaatccaca aatttctaaa gaaaactctg ttgctatgat gcgattttta
     2041 agtgctcttt ttattattat ctcatatttt attgcccgat atgaatttgc cataatcatt
     2101 acactcatgt cactttcatg gggagctgtt gccggtgctt ttatggcacc atacctgtac
     2161 ggtcttttct ggaagcgcac aactcttgca ggagtatatg caggtatgat tacagggctt
     2221 gtaacgtcaa ttggcctgtt tttctattta ggtgcaaacc tttcacctat agcttcaagc
     2281 gcagggatgc tggcaccttt tgttgtggtt ccacttgtta gcttgtttac taaaaagcct
     2341 tctgacgaaa caattacaaa agcctttgaa aagattggat aatgttgttg tatcaagtat
     2401 aatcataaaa gttattatct ttaaaagggg cacagaatgg acttcatacc attagaaaac
     2461 ataactatgg aactggctga tgttgccatg ggtcgccgca aggctgatat ggtaataaaa
     2521 aacggaacac tggtaaatgt aaatacaaaa gaattgcagg aacatattga tgtagcacta
     2581 tataaaggac gcattgcact ggttggcgat gcaaagcact gtattggtga gtcaacggag
     2641 atagttgatg caacaggact ttttatagcg ccaggtttca tggatggaca tattcacgtt
     2701 gaaagcagta tgctcaccgt gagtcagtac agtaacgctg tggtacccca cggtactacg
     2761 gcaatcttca tggaccctca tgagattgcc aatgtactgg ggcttaaagg cgtaagactt
     2821 atgcttgatg aagcaaaaga tgtgcctctt agagtatatg ctacggttcc ttcatgcgtc
     2881 ccttctgcac cggggttaga agatgcagga gcagttattg gagccagtga cattgaacag
     2941 gccatgcagt gggagggtat tgccgggctg ggtgagctta tgaattttcc cggagttttc
     3001 aacagtgtcc cggaagtaca caaagaaata gaagtaacat taaaggctgg caaacctgtc
//
LOCUS       DAOSZP010000248         3041 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_25340_length_3041_cov_3.139317, whole genome shotgun sequence.
ACCESSION   DAOSZP010000248 DAOSZP010000000
VERSION     DAOSZP010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3041
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25340_length_3041_cov_3.139317"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..572)
                     /locus_tag="PK348_06270"
     CDS             complement(<1..572)
                     /locus_tag="PK348_06270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65854.1"
                     /translation="MSLTIFDDIYNEIRKRNTAGGFKGVPYSDEFVKYVSGYYGLSID
                     LIKRIIQILINAHKIFSIEIIAEDKVRDISRVEGYVVADLVVIRKLKSFYQSELIIQY
                     ENEFHKRLMVHQIVKEIFPMLRSLNNTDIGKVANLAIMLEELERYMEKHYDEFTEEWK
                     AKRLAYEISISNIDLQLERKQSQKGKDDGQG"
     gene            743..817
                     /locus_tag="PK348_06275"
     tRNA            743..817
                     /locus_tag="PK348_06275"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:778..780,aa:Asp,seq:gtc)
     gene            complement(849..1838)
                     /gene="moaA"
                     /locus_tag="PK348_06280"
     CDS             complement(849..1838)
                     /gene="moaA"
                     /locus_tag="PK348_06280"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR02666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HXK65855.1"
                     /translation="MLVDSFGRTIDYVRISVTDHCNLRCVYCMPADIKWLPHEEILRN
                     EEIVRLVDVFVSLGIKKVRFTGGEPLLRKGFFDIVKQVRSNHKELEICITTNGILLEQ
                     YIDKIQEFNVQKVNVSLDSLNPDTFAKITGVNAIHKVINGILKITALEGVECKINAVI
                     MESTLDELEELVRFATNIHAIRFIERMPLSNDPVPFVSADTLLQKLESLGTVQRKTTS
                     DTHVAAMYRFLPRWDKRAVINVGIIPAVSHRFCGKCNRLRVTSDGMLRACLHDATEYN
                     LKKILRGQLNDNIREVITNAVLHKKEGHSLMQCDDTAFNCSGMAMHSMSKIGG"
     gene            complement(1839..2399)
                     /locus_tag="PK348_06285"
     CDS             complement(1839..2399)
                     /locus_tag="PK348_06285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65856.1"
                     /translation="MNYRTVLLIIFVILCAHTSFAQQPFTFVKTDEPVSLWKALAWDV
                     LPGGGHIYTGHYEYAAVFAGLKISGVYAMYYYYNYWQYRGSLYRSAKKANEAIDPEHD
                     LLFKDPEGGYKTVKEFGYAYDRAAHYFTLSILANAAIYITSWLCTWYHVQEINESRQP
                     TYVGFNQFSFTTDENGTMWASCTMRW"
     gene            complement(2489..2992)
                     /locus_tag="PK348_06290"
     CDS             complement(2489..2992)
                     /locus_tag="PK348_06290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65857.1"
                     /translation="MSLNNKTIVLLLAIVLATGCLHEPVDEEYFDDLSRVKGGQKPTP
                     PVDLSMTINNYIITLSFYTDKIDNGQLLTYDPDTGSNENLYYLVYISATDPAQFSDPK
                     LYYDIKYYVDYRAETDFEEGVDPKVVTIQISSSYHGPVYVWMTSHDGGRESDHSEVVN
                     GDIPEKS"
BASE COUNT          927 a          638 c          481 g          995 t
ORIGIN      
        1 ccttgaccat catctttccc tttctgggat tgtttccttt ctaactgcaa atcaatattg
       61 gatatactta tctcgtaagc tagacgctta gctttccatt cttcagtaaa ttcatcataa
      121 tgcttctcca tatacctttc aagctcttca agcatgatag ctaaattggc aaccttccca
      181 atatcagtat tgttgaggct tcgtaacatt gggaaaattt ctttaactat ctgatgtacc
      241 atcaagcgtt tatggaattc gttttcatac tgtataataa gttcactttg ataaaacgat
      301 tttaattttc gtatcacaac caggtctgca accacatacc cttccaccct ggaaatatca
      361 cggaccttat cctctgctat aatctcaata gaaaatattt tatgggcatt aataagtatc
      421 tgaatgatac gcttaataag atcaatagaa agtccataat aaccagatac atactttaca
      481 aattcatccg aatagggaac acctttaaat ccacctgcag tattacgttt tcggatttca
      541 ttataaatat catcaaaaat tgttaagctc attttacaac cttttattca tcaaaataaa
      601 aactcaaata tgatagccat catagatgat aaaaaaataa aagcgttttt tatatttttt
      661 ttgtcataat aaaaaaaatg tgattttttt tattgacaga tgttgacgtg attaacctat
      721 tgtagtatag ttttaatcaa aaggggtgtt agctcagctt ggttagagtg cctgcctgtc
      781 acgcaggagg tcgcgagttc aagtctcgta caccccgcat aaaaaaagcc gcttatatgc
      841 ggcttttttt atccaccaat ttttgacatt gaatgcattg ccatacctga acaattaaac
      901 gcagtatcat cacattgcat taatgaatgt ccttcctttt tatgtaatac ggcattggtt
      961 ataacttcac gtatattatc atttaattgt cctctgagta tcttttttaa attatattca
     1021 gttgcatcat gcagacaagc cctgagcata ccatctgaag ttacccgtaa acgattacat
     1081 ttaccacaga acctgtgtga cactgcaggt ataattccaa cattaataac tgcacgttta
     1141 tcccacctgg gcaaaaatct gtacattgca gcaacatgtg tatcgcttgt tgtcttacgc
     1201 tgcactgttc ctaatgattc caatttttga agtaatgtat ctgcactcac aaatggtacc
     1261 ggatcattac ttaacggcat gcgttcaata aagcgaattg catggatatt tgttgcaaaa
     1321 cgcaccagtt cttccagttc atcaagtgta gattccatta ttacagcgtt aattttacat
     1381 tctacaccct ctaacgctgt tattttcagt attccgttta taactttgtg tatggcatta
     1441 accccggtaa tttttgcaaa tgtatcagga ttaagagaat caagacttac atttactttc
     1501 tgaacgttga attcctgtat tttatcaata tattgttcaa gtaatatacc attagtagtt
     1561 atacatattt ctaattcttt gtggttgctt cgtacctgtt ttacaatatc aaaaaagcct
     1621 tttcgtagta acggttcgcc gccagtaaaa cggacttttt taattcccag agaaacaaac
     1681 acatccacca gcctcactat ctcttcatta cgcaaaatct cttcatgcgg taaccatttg
     1741 atatctgcag gcatacaata tacacatcgc aggttgcaat ggtccgttac agaaatacgc
     1801 acataatcta tagttctgcc aaagctatca acaagcattt accacctcat ggtacaggaa
     1861 gcccacatgg ttccattttc atccgttgtg aatgaaaact gattaaagcc tacatatgtt
     1921 ggttgcctgc tttcattgat ctcctgaaca tggtaccagg tacaaagcca ggatgtaata
     1981 tatatagcag catttgccag tatactcaag gtaaaataat gagctgctct gtcatatgca
     2041 tacccaaatt cttttactgt tttatagcca ccttccgggt ccttgaaaag taaatcatgt
     2101 tccggatcta ttgcttcatt tgcttttttt gccgatcgat acaacgaacc acgatattgc
     2161 cagtaattat agtagtaata catagcatag acacctgaaa tttttaaacc ggcaaaaaca
     2221 gcggcatatt cataatgccc cgtatatata tgcccaccgc ccggaagaac gtcccaggca
     2281 agtgccttcc ataaactcac cggttcatca gttttgacaa acgtaaaggg ttgttgcgca
     2341 aacgacgtgt gtgcacacaa aattacaaaa ataattaata atactgtgcg atagttcata
     2401 ctataaatcc attattatta taatcaacct attgaaacca tgcctggctt ctttaccgga
     2461 aaccacgcat ggcgactcta cacatacatt acgatttttc cggaatatca ccatttacca
     2521 cctcactgtg gtcgctttcc cttccaccat cgtgggatgt catccacaca tacaccggcc
     2581 catgatagga agaagaaatt tgtatggtaa caacctttgg gtcaacgcct tcttcaaaat
     2641 cagtttcagc acggtagtcc acgtaatatt tgatatcgta atataacttt gggtcgctga
     2701 attgtgctgg atcagttgct gatatataca ccagataata cagattttca ttgctgccag
     2761 tatctggatc ataggtgagt aattgtccat tatctatctt atcagtataa aatgaaagcg
     2821 taattatata gttattaatt gtcattgata aatcaactgg cggtgttggc ttctgaccac
     2881 ctttgactct ggaaaggtca tcaaagtatt cttcatcaac gggttcatgc aggcaaccgg
     2941 tagcaagaac tatcgccaat aacaatacta ttgttttatt gtttaagctc atgcaggtac
     3001 aggtaatttt ttctaaaata tggattatgc ggcttcagta c
//
LOCUS       DAOSZP010000249         3037 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_25389_length_3037_cov_2.712274, whole genome shotgun sequence.
ACCESSION   DAOSZP010000249 DAOSZP010000000
VERSION     DAOSZP010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3037
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25389_length_3037_cov_2.712274"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..559
                     /locus_tag="PK348_06295"
     CDS             <1..559
                     /locus_tag="PK348_06295"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HXK65858.1"
                     /translation="KNIVKIINDISEQINLLSLNAAIEAARAGDFGRGFAVVADEISK
                     LAEQTAISIKEIDGLIKQNSTEMAKGTQNITQAIQNIGFIINGVRDISKRIEQVAADM
                     KEQLAINTKVQGMISDVQAGSEEIKNATEEQRIAIMEISRSISTINELSQNNAGAAEE
                     MSANATGVEALAIKLKEMVSFFKIA"
     gene            707..1756
                     /locus_tag="PK348_06300"
     CDS             707..1756
                     /locus_tag="PK348_06300"
                     /inference="COORDINATES: protein motif:HMM:NF017847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HXK65859.1"
                     /translation="MGLSLGITGYPQSGKKTLLQILTKNSFDIKKGIGVATIHDERFT
                     TLVKHYNPRKTAPATINISLIGDLTDEAIREGNIFSNIATMDALCFVVRAFNDDSVYH
                     VKGSVDPLRDYDKLLSECILHDMVFAEKRIERLNQDKKKTAQQKEKEIMLLQTFMQHL
                     EQGNLLNSLKLADDDIAMIKSYPFITLKKIIVVFNTDDSGSEILDAFNRVYANHTVKA
                     VAVAVKLESEISLIDDEREQKEFYASMGVEKPALHVLCDAMLDSLGLQSFFTVGQDEV
                     KQWLIPKGITAPLAAGYIHSDIQRGFIRAELMHYDDFIVAGSEERLKKEGKYHLVGKD
                     YYMQDGDIVSFRFNV"
     gene            1845..2738
                     /locus_tag="PK348_06305"
     CDS             1845..2738
                     /locus_tag="PK348_06305"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HXK65860.1"
                     /translation="MDKLLSGLARVRESYRTKKNIDATIKKKFLSCIYEFYDRNYRHL
                     PWRNTDNPYHILVSEVMLQQTQVSRVVEKYSSFIKEFPTVETLAQASLHDVLATWQGL
                     GYNRRAKYLWQAAQIIVSQYNGKVPDNHHALHKLPGLGKATAASVAIFAFGRSELYIE
                     TNVRTVYIYSFFADNEKVSDAMIEPLLKVTIDTTNPKKFYNALMDCGTYIKQHYNFSR
                     HSAHYTVQTPYSTSLRKVRGDIVRYIVNNGAANNDMLVQSLGYAPEKIQKALEALCRE
                     NMVCEDNGIYTIPSHELIKNN"
     gene            2819..>3037
                     /locus_tag="PK348_06310"
     CDS             2819..>3037
                     /locus_tag="PK348_06310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65861.1"
                     /translation="MIKRLTVVFLLVFVLCTSAQVFAVPVAYRSWTYLDLTALLSPQW
                     SYTLLTSHSYEYERKNDPGKEEKKTFFYE"
BASE COUNT         1000 a          517 c          662 g          858 t
ORIGIN      
        1 gaaaaatatt gtgaaaatca tcaatgacat ttcagaacag attaatcttc tatcgctcaa
       61 tgcagctatt gaggcagcac gtgctgggga ttttggtcgc ggttttgcag tagttgccga
      121 tgaaatttca aaattagctg aacagacagc tatcagtata aaagagatag atggcctgat
      181 aaagcaaaac agcaccgaaa tggcaaaggg cactcaaaat attactcagg cgattcagaa
      241 tattggtttt atcatcaatg gtgtccgtga tatatcaaaa agaattgaac aggttgcagc
      301 tgatatgaag gaacaactgg caatcaacac taaagttcag ggcatgataa gtgatgttca
      361 ggcaggctct gaagagataa agaatgcaac agaagaacag cgtattgcaa taatggaaat
      421 ttcacggtca ataagcacta tcaatgaact atcgcaaaat aatgctggcg cagctgagga
      481 gatgtcagca aatgcaacgg gtgttgaagc actggcaata aaattaaaag aaatggtgtc
      541 attcttcaag atagcataat tttttgataa acatacagga tgtgttatgt atatacttta
      601 aaaaggcaca tggcctttta ttcacataag aaagattgct gagtcatcat cgcttgacgc
      661 aatgactata taacattcta tattgtaagg gagctttata actgcaatgg gcttatcact
      721 aggaattact ggttatccac agagtggcaa aaagacgtta ttacaaattt taacaaaaaa
      781 ttcatttgat ataaaaaaag ggattggtgt tgccacaatc cacgatgaac gttttaccac
      841 gctggtgaaa cattacaatc ccaggaagac agcacctgct acaattaata tttcactcat
      901 tggtgatttg acggatgaag ctattaggga aggaaatatt ttcagcaata ttgcaacaat
      961 ggatgcccta tgctttgttg tgcgcgcttt taacgatgac tcagtatatc acgttaaggg
     1021 cagcgttgac ccgctgcgcg attatgacaa gctactgagt gaatgtattt tgcatgatat
     1081 ggtgtttgca gaaaagcgga tagaacgtct taatcaggat aaaaagaaaa cagcgcaaca
     1141 aaaagaaaaa gaaattatgt tactgcaaac atttatgcag catctggaac agggcaatct
     1201 tttaaacagt ctgaagcttg ctgatgatga tattgcaatg attaagagct acccgtttat
     1261 tacattgaag aaaataatag ttgttttcaa tactgatgat agcggcagtg aaattctaga
     1321 tgcatttaat agagtttatg caaaccatac cgtgaaggca gtagctgttg cggtaaagct
     1381 tgaaagcgaa atatcgctga tagatgatga aagagaacaa aaagaatttt acgcttccat
     1441 gggtgtggaa aaacctgcat tgcatgtatt atgcgatgct atgcttgact ctcttggcct
     1501 gcagtcgttt tttactgttg ggcaggatga agtaaaacag tggcttatcc ccaaaggtat
     1561 cacagcgcca cttgcagcag gctatatcca cagtgatatt cagcgcggct tcatcagagc
     1621 tgagcttatg cattatgatg attttattgt agcaggttct gaggagcgcc ttaaaaaaga
     1681 aggaaagtat cacctggttg gcaaagacta ctatatgcag gatggtgata ttgtgtcatt
     1741 caggtttaat gtgtagcgat agtcatggtg tatgaactat tacttttaac catagccatt
     1801 gtctacattt taaaaacggc atattaattc tggcgatagt tactatggat aagcttcttt
     1861 caggacttgc acgggtacgg gagtcctatc gaacaaaaaa gaatattgat gcaacgataa
     1921 aaaagaagtt cttatcatgt atatatgaat tctatgacag gaattatcgc cacctgccct
     1981 ggcgaaatac tgataatccc taccacattc ttgtttccga agtgatgcta cagcaaacgc
     2041 aggtgagccg cgttgttgaa aaatattctt cgtttatcaa agaatttcca actgtggaaa
     2101 ctctggcaca ggcaagcctt catgacgttt tagctacatg gcagggactg gggtataaca
     2161 ggcgggcaaa gtatctgtgg caggctgcgc agatcattgt gtcacagtac aatggcaaag
     2221 tgccagataa tcaccatgca ttacacaaac ttcctggtct ggggaaggca actgctgctt
     2281 cggttgccat atttgcattt ggcagatctg aattgtatat tgaaaccaat gtgcgcacag
     2341 tatatattta tagctttttt gctgataatg aaaaagtatc tgatgcaatg attgagcctt
     2401 tattaaaggt aactatcgat actactaatc caaaaaagtt ttataatgca cttatggatt
     2461 gtggcacgta tataaagcag cactataatt tttcgcgtca tagcgcacat tacacagtac
     2521 aaacgccgta cagtacttca ttgcgaaaag tgcgcggtga tatagttcgg tatattgtaa
     2581 acaatggtgc agcaaataat gatatgctgg tgcaaagcct gggatatgcg cctgaaaaaa
     2641 tacagaaagc acttgaggcg ctgtgccggg aaaacatggt atgcgaagat aacggcatat
     2701 atacgattcc cagccatgag ttgataaaaa ataattgaca aaatgattac aatgttgatt
     2761 gactataatg agggattagt ttaattccat attattttta tacatagaga ggtttcccat
     2821 gataaaacga cttactgtag tattcttgct ggtgtttgtt ttgtgtacat cagcgcaggt
     2881 atttgctgta ccagttgcat accgttcgtg gacgtattta gatttaacgg cactgctttc
     2941 gccacagtgg tcatatactc tgttaacaag ccatagctat gagtatgaaa gaaaaaatga
     3001 tccaggtaaa gaggaaaaaa agacattctt ttacgaa
//
LOCUS       DAOSZP010000250         3036 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_25396_length_3036_cov_2.955384, whole genome shotgun sequence.
ACCESSION   DAOSZP010000250 DAOSZP010000000
VERSION     DAOSZP010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3036
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25396_length_3036_cov_2.955384"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..386
                     /locus_tag="PK348_06315"
     CDS             <1..386
                     /locus_tag="PK348_06315"
                     /inference="COORDINATES: protein motif:HMM:NF019292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HXK65862.1"
                     /translation="TFIGKTGLLIYPLLKPLGFNWQMGVSLTTGFVAKEVVVSTMGVL
                     YHATDDESNQNLSQKLKNPRYGISKASALAFMIFVMIYIPCLATVIAIAREIGLRWAV
                     FSIFYQVFVAWIVSFALYHVARLII"
     gene            complement(387..566)
                     /locus_tag="PK348_06320"
     CDS             complement(387..566)
                     /locus_tag="PK348_06320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65863.1"
                     /translation="MNKKDEGKGYIYHIEEEVLQEYQKKPIYLRLKWLYMGNYLRMGY
                     NKKIKDTHDVFRKGT"
     gene            complement(550..1047)
                     /locus_tag="PK348_06325"
     CDS             complement(550..1047)
                     /locus_tag="PK348_06325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65864.1"
                     /translation="MLDYLGIFKAFNDRGIHYIVVGGIAVNLYGIPRMTYDIDILLEM
                     SEHNIKQFIGLIKEWGFKPRVPVSVDDFANEDKRKDWIHNKNMKAFTFINPEWAISEI
                     DVIIDSPIDYIHAYNNAVAITVGDITIPVVSIDDLIDMKRKSDRKHDQIDVEYLERVK
                     NEQKG"
     gene            complement(1050..1964)
                     /locus_tag="PK348_06330"
     CDS             complement(1050..1964)
                     /locus_tag="PK348_06330"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HXK65865.1"
                     /translation="MKKRIVWLIIIVIVVISVLFAAGYYFSGMVITPNVWNYEETYRN
                     ELQYKRFTEHYYANLPKEEFFIDSPNGYKLHAMYIPKGSNKTVIFIHGHTYTLMGDIK
                     YLEIFRSLGFNALLFDSRFHGKSGGSNTTFGKYEKYDVKACVDWISKKTGEYSIIGIH
                     GESMGSAICLLYAAIDEKCSFFIVDGAMADLHELLAYRLKKDFCLPAFPLLHSASLIS
                     GLRGAMFFSEISPVTIINNIKAPILFIHGGNDTDIPVEHAQRLYNRYTGIKRIWICPG
                     ATHSKSIVVNSEEYEKQVKEFLHAIGVI"
     gene            complement(1975..>3036)
                     /locus_tag="PK348_06335"
     CDS             complement(1975..>3036)
                     /locus_tag="PK348_06335"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HXK65866.1"
                     /translation="ICRKTGARPIYLDEDKTVLFEFKGKSSSQYNHDGYDLTKFEMPV
                     TVVDKLINGKNENLYINVPKLKTHSMAIVTLGVKNQWAFPRHNDRRYDHNYNLHYKLV
                     DILGYVQPDFTLIEGIEGTIYGHYPVTKLADTCVIPFKVLIGGDNVVAADIVASRIFG
                     LDVKDVPHLHIAVKKGFAKGVKSIKDIKIDGSLKEYKKRYPTDLYESFPPDVDIIMGK
                     ELCCKEGCRNNPLTLLQVLYHDYNGKGGWSLVIGKGHNKEFIKSIRKPVLIAGHCAIE
                     ETYTMLVQQLGKGNVYVSGQCNDLAASAAAMFKLMKVNPLKLVNLSPLKSVKLLLLAK
                     LHGTKANVPNLLSRFIKTV"
BASE COUNT          936 a          628 c          454 g         1018 t
ORIGIN      
        1 atacgttcat tgggaaaaca ggcctactta tttatccact gctgaaacct ttaggattta
       61 actggcaaat gggtgtttcg cttactacag gttttgtggc aaaagaagta gtagttagca
      121 ccatgggcgt tttataccat gcaactgatg atgaaagcaa tcagaacctt tcacaaaaac
      181 taaaaaatcc ccggtacggc atatcaaaag caagtgctct tgccttcatg atatttgtta
      241 tgatatacat tccctgcctt gcaaccgtta ttgccattgc ccgtgaaatt ggcctacggt
      301 gggcagtttt ttcaattttc tatcaggttt ttgtggcatg gattgtgagc tttgcattgt
      361 atcatgtggc acgattgatt atataattat gtgccctttc tgaagacatc atgtgtatct
      421 tttatttttt tattatatcc cattcgcaaa taattaccca tatatagcca cttaaggcgc
      481 aaatatattg gcttcttctg atattcttgt aaaacttctt cttctatatg atatatatac
      541 ccttttccct catccttttt gttcattttt taccctctcc agatattcaa catcaatctg
      601 atcatgtttt ctatcgcttt ttcgtttcat gtcaataaga tcgtcaattg acacaacagg
      661 tatagtaata tcaccaaccg taatagcaac tgcattgtta taagcatgta tataatcaat
      721 tggagaatct atgataacat caatttcaga aattgcccat tcaggattta taaaagtaaa
      781 tgctttcatg tttttattat gtatccaatc ttttcgttta tcttcatttg caaaatcatc
      841 aacggaaact ggtactctgg gtttaaaacc ccattcttta ataagaccaa taaattgctt
      901 aatattatgt tcactcattt ccaaaagtat atcaatatca tatgtcatac gcggtatgcc
      961 atatagattt acagcaattc ctccaaccac aatatagtgt atacctctat cattaaatgc
     1021 tttaaatatc ccaagataat ccagcattat caaatcaccc caatggcatg caaaaattct
     1081 tttacctgct tttcatattc ttcgctgttt actacaattg attttgaatg tgttgcgccc
     1141 gggcaaatcc atattcgttt tatccctgta tacctgttat acaaacgttg tgcatgttca
     1201 acagggatat cagtatcatt acccccatgg ataaacaata ttggtgcttt aatattgtta
     1261 attattgtga caggtgatat ctcagaaaaa aacattgcac cccgtaaccc gcttatcagg
     1321 cttgctgaat gcaataatgg aaatgccggc aaacaaaaat cttttttaag acgatatgcc
     1381 agaagttcat gtaaatcagc cattgctccg tcaacaatga aaaagctgca tttttcatca
     1441 atggcagcat ataataaaca aattgccgat cccattgact cgccatgtat gccaataatg
     1501 ctatattcac cagttttctt gctaatccag tcaacacagg ctttaacatc atatttttca
     1561 tatttaccaa atgttgtatt ggaaccacca cttttgccat gaaatctact atcaaacaac
     1621 aatgcattga accccaatga acggaatatt tccagatact tgatatcgcc cattaaggta
     1681 tacgtatgtc catgaataaa tataactgtt ttattgctac cttttggtat atacattgca
     1741 tgcagtttat agccattagg cgaatcaata aagaattcct cttttggcaa attagcataa
     1801 tagtgttcag taaatctttt atattgcaat tcattacggt acgtttcttc ataattccac
     1861 acgttaggtg taataaccat acctgaaaaa taatacccgg cagcaaaaag aactgaaatt
     1921 acgacgatga ctattataat gagccataca attctttttt tcatgtacta tctcctaaac
     1981 tgttttaata aaacgcgata gtaaatttgg tacatttgct tttgtaccat gtaattttgc
     2041 taagagcaac aactttaccg attttaaagg agacagattc accagcttta aaggatttac
     2101 cttcatcaac ttaaacatag cagcagccga agcagccagg tcattacatt gtccactgac
     2161 atatacatta cctttgccca attgttgtac cagcatggta taagtttctt caattgcaca
     2221 atgcccagca atcagtactg gcttacgaat acttttaata aattctttat tatggccttt
     2281 gccaataaca agactccagc cacccttgcc attgtaatca tggtataata cctgtaaaag
     2341 tgttaaagga ttattgcggc atccttcctt acagcacaac tcttttccca taataatgtc
     2401 aacatcagga gggaagcttt catataagtc agtgggatag cgtttcttat actccttaag
     2461 actgccatct atcttgatat ccttaataga tttaacacct tttgcaaaac ccttttttac
     2521 tgctatgtga agatgcggca catctttaac atccagccca aaaatacggg aagcaacaat
     2581 atctgcagcc actacattat caccacctat taacacttta aatggtatga cacaggtatc
     2641 agccaatttt gttacgggat agtgcccgta gattgttcct tcaatccctt caatgagcgt
     2701 aaaatcaggc tgcacatatc caagaatatc cacaagctta taatgaagat tatagttatg
     2761 atcgtacctt ctgtcattat gccgtgggaa agcccactga ttctttacac ccaatgttac
     2821 aatagccatt gagtgagtct taagttttgg cacatttata tatagatttt cgtttttacc
     2881 gttaataagc ttatctacaa ctgttactgg catttcaaat ttagtcaggt catagccatc
     2941 atgattatac tgcgatgaag atttcccctt aaattcaaaa agaactgttt tatcctcatc
     3001 cagatagata ggcctggcac ctgttttacg gcatat
//
LOCUS       DAOSZP010000251         3035 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_25402_length_3035_cov_3.128523, whole genome shotgun sequence.
ACCESSION   DAOSZP010000251 DAOSZP010000000
VERSION     DAOSZP010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3035
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25402_length_3035_cov_3.128523"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1176)
                     /locus_tag="PK348_06340"
     CDS             complement(<1..1176)
                     /locus_tag="PK348_06340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4350 domain-containing protein"
                     /protein_id="HXK65867.1"
                     /translation="MKRILFILLFIISCSQKYTDYTPFTIQLIYDQSHNNVWGLWDHG
                     YCGYSHIAGVLKQHGIPASVNLKKLDECISSLPSMGYSNAILMLSVAKYQRFDDREID
                     AISNFVNAGGLLFVIAEHDNMYNSATFLNAVTHKMGITINSDAAGKDNDAFQSLDDLN
                     NRAYSKKFKLKNVLHMLSASLSGTADSFEVLLRDDETDNIIAGGCRYGKGKMLVVGDS
                     EMFWNGDGKVGIHAGDNSAFFKHCIEWLLEKHLAHTVQHAVTVPCFDAENSYIAIDKS
                     PSGVEHFINALTKINPSLLHDDSINIIVIPMPNCGINNTNRNIIFIEPYQKLIPSTVW
                     GKRLIALGAVNTKVYSFLGDSNCEILPCLITDGDTNFFDVVISCNKKRFYFHRLAGMI
                     "
     gene            complement(1222..2586)
                     /locus_tag="PK348_06345"
                     /pseudo
     CDS             complement(1222..2586)
                     /locus_tag="PK348_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873389.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
BASE COUNT          931 a          668 c          508 g          928 t
ORIGIN      
        1 tatcattcct gcaagcctgt gaaaataaaa cctcttttta ttacaggaaa tcacaacatc
       61 aaaaaagtta gtatcgccat cagttataag gcagggaagt atttcacagt tgctatcgcc
      121 caaaaacgaa taaaccttag tatttacagc accaagcgca ataaggcgct tgccccatac
      181 cgttgatggt ataagttttt gatatggttc aataaaaatg atatttcgat ttgtgttgtt
      241 aataccacaa ttgggcatgg gaatgactat tatattaatt gaatcatcat gcagtagtga
      301 ggggtttatt tttgtgagtg cattaataaa atgctctaca cccgatggcg atttgtcaat
      361 agcaatatat gaattttcag catcaaaaca tggtacagtt acagcatgtt gtacggtgtg
      421 agccaaatgc ttttccagaa gccactcaat gcagtgttta aaaaaagcac tgttatcgcc
      481 agcatgtatt ccaaccttgc catcgccatt ccagaacatc tcgctatcac ccaccacaag
      541 cattttgcct ttgccatacc tgcatccacc agcaataatg ttatctgttt catcatcacg
      601 caataacacc tcaaaagaat cagcagttcc cgatagcgaa gctgaaagca tgtgcaaaac
      661 attttttagt ttaaattttt ttgaatatgc cctgttattc aaatcatcaa gcgattggaa
      721 agcatcattg tctttcccgg cagcatcgct gtttattgtg atacccattt tatgcgttac
      781 tgcatttaaa aacgttgcac tattgtacat attatcatgt tcagcaatga caaaaagaag
      841 acccccagca ttaacaaaat tgctgatggc atcaatttca cgatcatcaa accgttgata
      901 tttagcaacc gacagcatca gtattgcatt gctgtatccc atggaaggca acgaagatat
      961 acactcatca agttttttca aattaactga agcagggata ccatgctgtt ttagcacacc
     1021 agcaatatgc gaatacccgc aatacccatg atcccacaat ccccacacat tattgtgtga
     1081 ttggtcatag attagctgta tagtaaatgg tgtgtaatcc gtatattttt gggaacagct
     1141 gataataaaa agcaggatga aaagtattct tttcatattc cataatggtg gggcgcacgg
     1201 taatgcgccc ctctaagaac attatttacc aacttttggc aaatcgtgca aagccttatc
     1261 aattaattct tttggatatt cgtaatcatc gagctcaccc gataaaaact tgtcatacga
     1321 tgccagatca aaatgaccgt gaccactcaa attaaatact atagtctttt gcttgccttc
     1381 ttcctttgct ttctgtgctt caatgatagc agctcttata gcatgagatg attcaggcgc
     1441 agggataata ccttcagtct ttgagaacaa aattgccgct tcaaagcatt ccttttgatg
     1501 caatgccatt gccgatataa gtccttcatg atacaagaaa ctcaccagtg gtgacatacc
     1561 atggtaacgc aatccgccag catgtattga aggagggata aatgagtgac ctaatgtata
     1621 cattggcata attggcgtaa gttttgcaac atcaccataa tcatacgtat acatgccttt
     1681 tgttaatgta ggacatgctg caggttctac tgcaatgata tcgatatttt taccgttcat
     1741 tttatccctt acaaatggaa atgcaagccc agcaaagttt gaaccgccac caacacaacc
     1801 aataacaaca tcaggataat catcaaccat ctcaaactgt tttaatgttt caaggccaat
     1861 aattgtctga tgcaggcaca catgatttaa cacactgcct aaagaataat tggtatcttt
     1921 gctgtttgca gcttcttcca ctgcctcact gattgcaatt cccaaactgc caggactatc
     1981 aggatcctgt ttttatattt cccgcccgct gttagtaaga ttgcttggac ttggtgtaac
     2041 cgttgctccc cacgtttcca tcataaactt tcgataaggc ttttgttcat agcttacccg
     2101 taccatatac acatgcacat caatgccaaa gaaattgcca gcaagtgaca gagcactacc
     2161 ccactgtccc gctccagttt cagtggctaa tttcttaatg ccttcaagtt tattataata
     2221 cgcctgagca actgctgtat taagcttatg gcttcccgca ggcgaaacac cttcatattt
     2281 ataataaata ttggcagggg ttcctaaagc tgcttctaac cgacgagccc tgaaaagcgg
     2341 tgaaggtctc cacaaacgat aaatatgcaa tatgtcttcg gggatatcaa tatatttttt
     2401 gggactaact tcctgttcta ttaatcccat ggggaaaata gcatgcaatt catcaggtgt
     2461 taccggttta ccggttgcag ggcttatagg cgggtccata gacttgggta aatcagccag
     2521 aatattatac cattgccgtg gcatctcctt ttcgggtaac tgtattttag ttgaatcctt
     2581 tgccattgta tcctcctatt tgaatcattt aatttttaaa aaatattgcg tactacaaat
     2641 aaaaaggcta cggttaacac atattatccg tagcctttaa ggatttccct attataaatt
     2701 aattacctca agcgcccgga tatatgttat cctggcgccg ccaccaccaa actaaaatag
     2761 ctattatatt aagtttaccc actaccatat aaataacgtt taattttctt acatcttcaa
     2821 ccatgcatca atcatacaac aaatgtcaac aattttttta tttacctttg ttactttcca
     2881 aaaagctttt gtgcctgaga aaccagaaga tcatatatgc catcaaatcc cccattactc
     2941 ataaccacca taactgtatg cttattctga atctgtttga atgcttcatg aactatctca
     3001 tcaacagaat caaatacata agctggaatg ctctg
//
LOCUS       DAOSZP010000252         3027 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_25489_length_3027_cov_3.128197, whole genome shotgun sequence.
ACCESSION   DAOSZP010000252 DAOSZP010000000
VERSION     DAOSZP010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3027
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25489_length_3027_cov_3.128197"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..709
                     /locus_tag="PK348_06350"
     CDS             <1..709
                     /locus_tag="PK348_06350"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HXK65868.1"
                     /translation="GIVILEGFGLTETTPVTNVNRPGMIQPGSVGPALKDTIIKIADD
                     GEILIKGPQVMKGYYKNPKATKEVFTKDGFFKTGDIGVIDEKGRLYITGRIKDIIVTA
                     GGKNISPQNIENSLKESKYIEQVAVIGDRRKYLSALVIPAFEELGKWAKKQGIQFTNN
                     KELIENDKVNELIAQEIQKYTKQFSRVEQIRKFKLLEAEWSQASGELTPTLKVKRRVI
                     EQKYAKEIESMYPKDMD"
     gene            complement(765..2216)
                     /gene="ahcY"
                     /locus_tag="PK348_06355"
     CDS             complement(765..2216)
                     /gene="ahcY"
                     /locus_tag="PK348_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006968265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HXK65869.1"
                     /translation="MIQPLRKDTTSIPEFDAKIPYKVKDISLAELGRKELQLAENEMP
                     GLMALRQKYGSQKPLKGMKISGSLHMTIQTAMLIETLHHLGADIRWASCNIFSTQDHA
                     AAAIAKAGTAAVFAWKGETLEEYWWCTEQALIWPDGSGPDLIVDDGGDATLFVHMGVK
                     AEKDPSILDQEYDSKDMQCLIDRLRLSVENNPKRWTTIASKIRGVSEETTTGVHRLYQ
                     LEKTGELLFPAINVNDSVTKSKFDNLYGCRESLADGIKRATGIMVAGKVVVVCGYGDV
                     GKGCAQSMRGFGARVIVTEIDPICALQAVMEGYEVKTLEDVVEIGDIFVTATGCCDVI
                     RGEHMERMKDEAIVCNIGHFDSEIEMSYLENNKKCKRINIKPQVDKWILESGRSIIVL
                     SEGRLVNLGNANGHPSFVMSTSFTNQTLAQIELATKKLEKKVYTLPKKLDEEVARLHL
                     GRLGVKLTRLTPKQAAYLGIEVDGPYKPNHYRY"
     gene            2328..2855
                     /locus_tag="PK348_06360"
     CDS             2328..2855
                     /locus_tag="PK348_06360"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HXK65870.1"
                     /translation="MSKKNYRILIFVLMMTLISCVPSQWQYRNIPLKRKQIINTATQY
                     IGTPYRHGGATPSGFDCSGYVFYVYNKNGIQIPRSTAQQYTKGQKIPYKMAQPGDLLF
                     FRINMNTISHVGIYVGNDTFVHAPSSGKYVRYDSINNTYWKKRFCGAVSYFKALPVKP
                     LNNKGDSYREERFVW"
     gene            2943..>3027
                     /locus_tag="PK348_06365"
     CDS             2943..>3027
                     /locus_tag="PK348_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011917311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HXK65871.1"
                     /translation="MQIGIYPGSFDPLTCGHLDIIKRATQLF"
BASE COUNT          947 a          573 c          565 g          942 t
ORIGIN      
        1 gggtatagtt atacttgagg gatttggcct tactgaaaca actccggtaa ccaatgtaaa
       61 caggccaggt atgatacagc caggatcagt tggacctgct ttaaaagata caattatcaa
      121 aattgcagat gatggggaaa ttttaataaa aggtccacag gtaatgaaag gatattataa
      181 aaacccaaaa gctacaaaag aagttttcac caaagatggt tttttcaaaa cgggtgatat
      241 tggtgtcatt gatgaaaaag gaagattgta tataactggc agaattaaag atatcattgt
      301 gactgcaggt ggtaaaaata tttccccaca aaatattgaa aatagcctga aagaatcaaa
      361 gtatattgaa caggtagcag taatagggga taggcgaaaa tacttatccg cccttgttat
      421 tccagctttt gaggagcttg gcaaatgggc taaaaaacaa ggtatacaat ttacgaataa
      481 taaagaactc attgaaaatg ataaggtaaa tgagttaatt gctcaggaaa ttcagaaata
      541 tacaaaacaa ttttcacgtg ttgagcaaat aagaaaattt aaactgttag aagctgaatg
      601 gagtcaggca agcggtgaac ttacaccaac attaaaagta aaacgccgtg ttattgaaca
      661 gaagtatgca aaagaaatag aatccatgta tccaaaagat atggattaac aatcagttga
      721 atataaaacg ggcaggttaa ccacctgccc gttttattat tctattaata tcggtaatga
      781 ttaggtttgt atggaccatc aacttctatt cccagatatg cggcctgttt tggtgttaat
      841 cgtgttaatt ttactcccaa tcgccctaaa tgtaatcgtg caacttcctc atctaatttt
      901 tttgggagtg tatatacttt cttttccagc ttctttgttg ccagttcaat ttgggcaagt
      961 gtttgattgg taaaacttgt gctcataaca aaacttgggt gaccgttagc atttcctaag
     1021 ttgacaagcc ttccttctga aagaacaata attgaccttc ctgattccag tatccattta
     1081 tcaacctgag gcttaatatt gattcttttg cattttttgt tattttccag atagctcatt
     1141 tcaatttcac tgtcaaaatg cccaatgttg cacactatcg cctcatcttt cattctttcc
     1201 atatgctcgc cccggataac atcacagcag cctgttgcag tcacaaagat gtcgccaatt
     1261 tctacaacat cctccagtgt ttttacttca tacccttcca ttacagcctg cagggcacag
     1321 attggatcaa tctctgtaac tatcactcgt gcaccaaatc cccgcataga ttgagcgcaa
     1381 cctttgccaa catcaccgta accgcatact actacaactt tgcctgcaac cataataccc
     1441 gttgcacgtt taatcccgtc tgctaaagat tctctgcatc cataaaggtt gtcaaatttg
     1501 gattttgtta ccgaatcgtt aacattaatt gctgggaaga gaagttcacc agttttctca
     1561 agctgatata gccggtgaac accggtagtt gtttcttctg aaacacctct gatttttgat
     1621 gcaatagtag tccaccgttt gggattgttt tctactgaca agcgcaatct gtctataagg
     1681 cattgcatat ctttgctgtc atattcttga tccagtattg atgggtcttt ttcagcttta
     1741 acgcccatat gaacaaatag tgttgcgtca ccaccatcat caacaataag gtcaggcccg
     1801 cttccatcag gccatataag ggcctgttcg gtgcaccacc agtattcttc aagggtttcg
     1861 cctttccatg caaagacagc agcagttcca gctttggcta tagcagcagc cgcatggtcc
     1921 tgtgtagaaa aaatattaca ggatgcccag cgaatatctg ctccaaggtg atgaagtgtt
     1981 tcaataagca ttgctgtctg tatggtcatg tgaaggctgc cactgatttt catgcctttt
     2041 aatggtttct ggctaccata tttctgacgt aatgccatga gtcctggcat ttcattttca
     2101 gctaattgca actccttcct tcctaattct gccagtgaaa tatcctttac cttataggga
     2161 atttttgcat caaattcagg aatactggta gtatctttac gtaatggctg aatcatatta
     2221 cacctctttt gtattaattt tcttatatta tgtattactc tatccaaact agtggagtgt
     2281 atattgtcaa tataaatatt atagcaacct atgacgatgg taatagtatg agtaaaaaaa
     2341 attatcgtat tttaatattt gtattgatga tgacattaat ttcatgtgtg ccttcacagt
     2401 ggcaatacag gaatataccc ttaaaaagaa aacaaataat caatactgca acacaatata
     2461 ttggcacacc ctacaggcac gggggtgcca caccatcggg ctttgattgt tcgggttacg
     2521 tgttttatgt gtataacaaa aatggcattc agataccacg aagtacagca caacaatata
     2581 caaaagggca aaaaatacct tataaaatgg cccagccggg ggatctactc tttttcagga
     2641 ttaacatgaa tacaatatca catgttggca tttatgttgg aaatgataca tttgtacatg
     2701 ctcccagcag cggaaaatat gtgcggtatg attccatcaa taatacatac tggaaaaagc
     2761 gtttttgtgg agctgtctcc tattttaaag ctttgcctgt aaaaccttta aataataagg
     2821 gcgatagtta tcgggaagag cgctttgtgt ggtagaaaaa aatattgaaa tatgaataaa
     2881 tgttgacaaa atgtaattat aagattattt ttgttgaacc gatcaggata ggttagcatt
     2941 atatgcaaat tggaatttat ccgggatctt ttgatccttt aacgtgtgga cacctggata
     3001 taattaaaag agcaacacag ttgtttg
//
LOCUS       DAOSZP010000253         3023 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_25532_length_3023_cov_3.359164, whole genome shotgun sequence.
ACCESSION   DAOSZP010000253 DAOSZP010000000
VERSION     DAOSZP010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3023
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25532_length_3023_cov_3.359164"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(176..1528)
                     /locus_tag="PK348_06370"
     CDS             complement(176..1528)
                     /locus_tag="PK348_06370"
                     /inference="COORDINATES: protein motif:HMM:TIGR00792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXK65872.1"
                     /translation="MKTTSDSLSINTKIGYGVGDFGANMVFQSVVLYLMFYLTDVFLI
                     PASVAGTIFLIAKIWDAVSDPMMGYISDKTRSRWGQKRPYLLFGAIPLGLSMALLFYT
                     PSLGDEAKILYALVMFLLVCTAYTVVNIPYGALTANLTLDSTERSRLTGYRMFCAVVG
                     TLLVAGATKPLAGLFQNPVEGWRFVGVLYGAIAAVFTLITFASVRERVQHSETEEYRL
                     KDIATTLSVNKPFIFLSIGVFMHLCAIGVLASMVNYLFKYNFNKEDFIPIAFLSMFAT
                     AAIAIPLWVFISNKIGKKHAFNIGMGLLAVILVVLYFAKQFNFAFFTVLFVAAGVSVS
                     TVFFSPWAMIPDTVEYSQWKTGLRREGIIYGFFYFSQKLAAACAGFITGIGLTLCGYI
                     QPKLINNILTAQIQSCDTLTGLKILTTLIPLALIICGIVFISLYPIDEKMHKDIVQKI
                     "
     gene            complement(1868..>3023)
                     /locus_tag="PK348_06375"
     CDS             complement(1868..>3023)
                     /locus_tag="PK348_06375"
                     /inference="COORDINATES: protein motif:HMM:NF014132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HXK65873.1"
                     /translation="KIFKVTDNYAFYPPIRLAKKIIENGEIGNPIAIRIKFIGGGSGG
                     WYVPPEAWQWRLKENTESGGVRGFDTFDHGHHLWTTAWYLCGNVERASGWIDSIDGII
                     DAPSVMIWKHKNGVYGSIEFVHMPALKIPSKYYANDEWIEVSGTQGMVIIHRCTGNIV
                     KGPAVSVFTTKGWKHYNEKSDWALGFIGATHNFIESILGKAQPVLSGADARDILRFSL
                     AIQKSAKVHREVYTEELDASFPSLYYYLRHRKDIAAAKPKKSFLERIGLSGNTSHYAP
                     QAKELTDEFLKRYNPDAVKQWTVTIALNIEPDGETKGFCYSIMINNSVLTAKEGELPQ
                     KWDMKITVPAGLWAAILLKKKRIEMAYLQGRIKIEGKSEEALKLRSAFGI"
BASE COUNT          897 a          709 c          533 g          884 t
ORIGIN      
        1 cgaatatgga gaattggatg agctgttttt ggaaattatt ccccagcctc atttatttaa
       61 ttataattga aaattgaaac ttttgaaaaa gactacttat ccttcccatt ctttgcatgg
      121 tgagtttatt tggcgggaac aactttttgt aacattaatg gtaagacaaa acaaattaaa
      181 tcttttgcac aatgtcctta tgcatctttt catctatggg ataaagactt ataaaaacaa
      241 tcccgcatat aattaatgcc agtggtatca gtgttgtaag aattttgagt cctgtaagtg
      301 tatcacaact ttgaatctgt gcagttaata tattgtttat taattttggc tggatgtacc
      361 cacacagcgt aagcccaata ccggtaataa accctgcaca tgctgctgct aatttctggc
      421 tgaaataaaa aaagccatag ataatccctt ctcttcgtaa gcccgtcttc cactgtgaat
      481 actccacggt atcaggaatc atagcccacg gactaaaaaa tactgtggaa acgctcactc
      541 ctgctgcaac aaaaagtaca gtaaaaaaag caaagttaaa ctgttttgca aaatacagaa
      601 caaccagaat aacggcaagc aaacccatgc caatattgaa tgcatgtttt ttaccaattt
      661 tatttgaaat gaaaacccat aagggaatag caatcgcagc agtagcaaac atggataaaa
      721 atgcaattgg gataaaatct tctttgttaa agttatattt gaagagatag ttaaccatag
      781 aagccagcac ccctatagca cacagatgca tgaatacacc aattgaaaga aagataaacg
      841 gtttattcac tgatagcgtt gttgcaatat ccttcaatcg gtattcttct gtttcactgt
      901 gctgtacacg ctcccgtact gatgcaaatg ttatcaaggt aaaaacggca gcaatagctc
      961 cgtacaaaac tccaacaaaa cgccagccct caaccggatt ttgaaaaagc cctgcaagcg
     1021 gcttagttgc tccagctacc aataatgttc ccaccacagc acagaacatc ctgtaacctg
     1081 taaggcgtga gcgctcggtg ctatcaagag taaggtttgc ggtcaatgcg ccgtatggaa
     1141 tgttgacaac cgtgtacgca gtgcatacaa gcaaaaacat caccagcgca tacaatattt
     1201 ttgcttcatc cccaagtgaa ggcgtgtaaa acagtaacgc catggaaagc cccagaggaa
     1261 ttgcgccaaa aagcaggtac ggccgctttt gtccccacct gcttcgtgtt ttatcggaga
     1321 tatatcccat catggggtca ctcactgcat cccatatctt tgcaattaaa aatatagtac
     1381 cagccactga agctggtatt aaaaacacgt cggttaaata aaacatgaga tacaaaacaa
     1441 ccgactgaaa taccatatta gcgccaaaat ctcccactcc atatcctatc tttgtattta
     1501 tggataagct atctgatgtt gttttcatgt cccctttcct ccaaaattaa aagttacccc
     1561 ttactgtaat cctgaagata tatttccatt actgtcatcc tgaagtccta cattcccctc
     1621 tgtcatcctg aagcctatat ttccccatgt catcctgaag ccacgcagtg gctgaaggat
     1681 ctcattgcct tcagcataag attcttcgtc ccgattgcat cgggactcag aatgacatgg
     1741 tgattgtcat cctgaagccc tacattcccc tctgtcatcc tgaagccacg cagtggctga
     1801 aggatcttgc cgtttcaaac acaagattct ttacccctat tgcatcggaa ctcagaatga
     1861 ctttcaacta tatcccaaag gcagaccgca atttcaacgc ctcctcagat ttcccttcaa
     1921 ttttaatacg cccctgaaga tacgccatct caattctttt tttcttcagc aaaattgctg
     1981 cccacagacc cgcaggtacc gttatcttca tatcccattt ttgcggcagt tctccctcct
     2041 ttgccgttaa cacgctgtta ttaatcatta tgctgtagca aaatccttta gtctcaccat
     2101 caggttcaat attcagggcg atagttaccg tccattgttt cactgcatca ggattatatc
     2161 gctttaaaaa ctcatcggtc aattcctttg cctgtggagc atagtgtgat gtgttgccag
     2221 agagtccaat gcgctcaaga aaacttttct ttggttttgc tgctgcaatg tctttacgat
     2281 gcctgagata ataatacaat gatggaaatg atgcatcaag ttcttcggta tatacttccc
     2341 tgtgtacttt agcagacttt tgtattgcta atgaaaaacg caggatatca cgcgcatctg
     2401 cacctgaaag aactggttgt gctttgccaa gtattgattc aataaaatta tgagttgcac
     2461 caataaatcc caatgcccag tcggattttt cgttatagtg tttccaacct ttagtggtga
     2521 atacactcac agcaggccct ttgacaatat tgccagtgca tcggtgtata atgaccatac
     2581 cctgagttcc gcttacctct atccattcat cattggcata atatttggaa ggaattttaa
     2641 gcgccggcat atgcacaaac tcaattgagc catatacacc atttttatgc ttccatatca
     2701 tcacagaagg agcatcaata atgccatcga ttgagtcaat ccatccactg gcacgctcaa
     2761 cgttcccaca taaataccag gcagttgtcc acaggtgatg cccatggtca aatgtatcaa
     2821 atccgcgaac accgccactt tcagtatttt ctttcagtct ccattgccag gcttcaggtg
     2881 gcacatacca gccaccactt ccacccccaa tgaatttaat gcggatggct atggggttac
     2941 ctatctctcc gttttctata attttttttg caagccgtat gggtgggtaa aatgcatagt
     3001 tatcggttac tttaaaaatt ttg
//
LOCUS       DAOSZP010000254         3018 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_25588_length_3018_cov_4.713804, whole genome shotgun sequence.
ACCESSION   DAOSZP010000254 DAOSZP010000000
VERSION     DAOSZP010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3018
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25588_length_3018_cov_4.713804"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..542)
                     /locus_tag="PK348_06380"
     CDS             complement(3..542)
                     /locus_tag="PK348_06380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65874.1"
                     /translation="MKNLFKALLGSKNAAKESNGQDLKNTIPAEKHQQLKAEPEESQK
                     YSLSVKVASVMGESVYAEYNGVANTMVENRILEHFSETDYLDSDEIPIQFKMCKVFFH
                     KLVDIHEAAAIMSADQEAQPSANTRIDEKGGMICYPTDVKNCQSEGKISWPGGSIKLT
                     RILMPSVEDNKYHLVIINS"
     gene            complement(588..2477)
                     /locus_tag="PK348_06385"
     CDS             complement(588..2477)
                     /locus_tag="PK348_06385"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HXK65875.1"
                     /translation="MPKPKTTPAPKPAPKKYIRNSKIAILQEKIEGFDDESIVEIELP
                     DGSTFWAKNFSVFSGNNETIDINFYLDGIARISTKYGNISFSGDSLRVKGGIVTEGLT
                     NNSIAIHSPEGVSVPLTYYLSFYWPSGNISRGYLSETLTINGIEYPKDAKVEFPEDGN
                     LSYVKINGNQVQLKELQQYETSLFEAIRTDNPAGVKESLEKIHAAHTRYEDIKNSDNQ
                     IPLQVALSLGHMEIASLLIENGFSIDQKNAQGDSALHVFSAAGNIAVVQWLLDNRANV
                     NIKDKKGRTPFSLALLAYRTDIVEMLLKAGADIPIRDDNDDHAIFDIIEDLTLVIEEK
                     TRLERACSLVRILVNAGMDVNMKNSAGESLFMKTCSAKYPELVELMISHGANINDRDA
                     MENTPLHHAVTSGNIKITEILIAAGADINPVNREGETALTIAYENNQIELVKILVNGG
                     ADTSKLPQEADLTFKDENINKRLQEAAYDGNSELIGALLKRGADVNVAESEMGYTPLM
                     WASMKDYHNDSVQLLIEAGANLNARNSKGETALMVALRESSKLNAELLVEKGADFNIA
                     NNVGEIPIVMAAGYGYTNVVAKMIAMGADVNVKSRNGDTALSFARSYGSDDVVKLLLA
                     HGAVN"
BASE COUNT          797 a          640 c          594 g          987 t
ORIGIN      
        1 ttttagctgt taataattac cagatggtat ttgttgtctt caacggaagg catcagtatt
       61 ctggttagct tgatagaacc gccgggccat gaaatctttc cctcgctctg gcagtttttt
      121 acatcggtag gatagcaaat cattccaccc ttctcatcaa ttcgcgtatt agcagaaggc
      181 tgagcttcct gatcagccga catgatagct gctgcctcat gaatatctac caacttgtga
      241 aagaaaactt tacacatttt gaattgaata ggtatttcat cggaatcgag gtaatcggtc
      301 tcgctgaaat gttcaagaat acgattctca accatggtat tggcaactcc gttatattca
      361 gcataaacag attcgcccat tacgctggcc acttttaccg aaagcgaata cttttgcgat
      421 tcttccggtt cagcttttag ctgctgatgt ttttcagcgg gaattgtgtt ttttaaatct
      481 tgcccgttgc tttcctttgc agcattctta ctgcccaaaa gcgctttgaa aaggtttttc
      541 atctgcattt tttcttatag gtttaacatt cgttagttga aaacatatta gttaactgct
      601 ccatgagcta gcaggagttt aacgacatca tcggatccat aacttctggc aaacgacagc
      661 gctgtatcac cattcctgga ttttacattt acgtcggcac ccatggctat catttttgcc
      721 acaacattcg tatatccgta ccctgcagcc atgacaattg gaatttcccc tacgttatta
      781 gcaatgttga aatcagcgcc tttctcaact aagagttcag cattaagttt actggattcc
      841 ctaagtgcaa ccatcagggc agtttcgcct ttactgtttc gggcattcag gtttgctccc
      901 gcttcgatca gcagctgtac ggaatcgtta tggtagtctt tcattgaagc ccacatcaaa
      961 ggggtatagc ccatttcgct ttctgcgaca ttcacatctg caccacgctt taaaagcgct
     1021 ccaatcaatt cactgttgcc atcatacgca gcctcttgaa gccgtttgtt gatgttttcg
     1081 tctttaaaag ttaaatcagc ttcctgtggc aattttgaag tatcggcgcc accattcaca
     1141 aggattttaa ccagctctat ctgattgttt tcataggcaa tagttaacgc agtttctcct
     1201 tccctgttta caggatttat atcagcgcct gcggcaatca gtatttctgt aatttttata
     1261 tttcctgaag taacggcatg atgaaggggt gtgttctcca tggcatctct atcattgata
     1321 ttagcgccat gcgagatcat tagctcgacg agttcgggat attttgccga gcacgttttc
     1381 ataaacaggc tttcgcctgc gctgttcttc atgttaacat ccatgccagc gtttacaagt
     1441 attcttacca gactgcacgc tcgctccagc ctggtctttt cttcaattac cagcgttaaa
     1501 tcttcaataa tatcaaaaat ggcatggtcg tcgttatcat ccctgatagg tatatccgca
     1561 ccggctttga gaagcatctc aacaatgtca gtcctatagg caaggagagc aagggaaaat
     1621 ggcgtacgtc cctttttatc ttttatattt acatttgccc tgttatccag tagccattgg
     1681 actactgcaa tatttcccgc agcagaaaaa acatgaagcg ctgaatcacc ctgtgcattc
     1741 ttctgatcaa tgctgaaacc attttctatc aacaacgagg caatttccat gtgccccaga
     1801 ctaagagcta cttgcagggg aatttgattg tcagaattct taatatcctc gtaccgagtg
     1861 tgtgcagcat gaattttttc aagcgattct ttaacgcccg ctggattatc tgtcctaatt
     1921 gcttcaaaaa gagatgtttc gtactgttgc agctccttga gctgcacctg attaccattt
     1981 atttttacat agctaagatt tccatcctca ggaaactcaa cctttgcatc tttgggatac
     2041 tctattccat ttattgtgag ggtttcgctc aggtagcccc tgctaatgtt gccgcttggc
     2101 cagtaaaagg aaagataata tgtcaagggt actgaaactc cttctggtga atgaatcgca
     2161 atagaattgt tagttaaacc ttcagtcact atgccgcctt taacccgcag gctatcaccc
     2221 gaaaatgaaa tattgccata tttagttgaa atacgcgcaa tcccatcaag ataaaagttg
     2281 atgtctatgg tttcattgtt tccactaaat acagaaaaat tttttgccca gaaggtagag
     2341 ccgtcaggca attctatttc aactattgat tcatcatcaa aaccttcaat cttttcttgt
     2401 agaatagcaa tctttgaatt acgtatgtat tttttaggcg ctggttttgg cgctggagta
     2461 gttttgggct taggcacagg ttctggaatt ttctcgctga gtggttcagc aggagtttcc
     2521 acaataggcc tttccttatc tttttcaatt ggctccttgg acttatcgat attgtcagca
     2581 atggaattgg gaaggagttc tctatctata ggagaatcgc gattgatgct tgttttgctc
     2641 gacgagaaca gctttttaat aatgctcata gtgatttgat tgaggtgtgg tttgcctagc
     2701 tatttgagac gttaaattta aataattatg ctgaattgtt aatgaaaatc taaaaaaaat
     2761 gggcttaatg gacatttttc aggctgacat agtgtttaac tacaataaat atcgaacttt
     2821 ggaggatttg aaagccatga attaaaattt gcttttactg aggagggtat tgtatgtgat
     2881 gggagaaaag gtttactctg ggcattgtaa gactatcctg ttcaaatgtg ccggtacccc
     2941 caagaggcca ttgtaatgcg gtatattaga taaaactaaa agttcaggca tcgaaagagc
     3001 taaaataaat cgttcatt
//
LOCUS       DAOSZP010000255         3017 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_25601_length_3017_cov_3.469953, whole genome shotgun sequence.
ACCESSION   DAOSZP010000255 DAOSZP010000000
VERSION     DAOSZP010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3017
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25601_length_3017_cov_3.469953"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..491)
                     /locus_tag="PK348_06390"
     CDS             complement(<1..491)
                     /locus_tag="PK348_06390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65876.1"
                     /translation="MKKKYLLCMFLVYFIPLTGINAQIPQGQLHSMIVKDETLIIPGI
                     GASSTVIDMSEQEVLLIKGNPFKKTQHVTHDFLKDVLKIQSEASLPFHYLYTYRNPLT
                     IIGFYNGRVSFVVVWDSYGVLIDGVRVSKGIMAIIFNYGNEGMQVLKDADGSIYMYSL
                     KGIA"
     gene            complement(497..1315)
                     /gene="rsmG"
                     /locus_tag="PK348_06395"
     CDS             complement(497..1315)
                     /gene="rsmG"
                     /locus_tag="PK348_06395"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF014575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HXK65877.1"
                     /translation="MTISNTVIIPDEINRKLKEYARLIYEHNKTTNITGLKDTESIYN
                     ELILGSITPLKHVNVPRGTSFIDIGTGAGIPGIPLLLYFGGNIKGVLVDSNNKKIKFL
                     LNAINTLGINDIVTVIQGRVEEIAKEKAYRDSFDFAVARAFAHPLMALEYGLPFVKPG
                     GWLYIYYSFNLYEKGFENNKTVCSNDEVIAEYGCDKLHEEKNANKHDITVLTDYLTKH
                     SLNLGGHRVTLEEAKKLKLYEGLIFLKDGYTPANYPRKHAVVKREVESLRNISS"
     gene            complement(2077..>3017)
                     /locus_tag="PK348_06400"
     CDS             complement(2077..>3017)
                     /locus_tag="PK348_06400"
                     /inference="COORDINATES: protein motif:HMM:NF025300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXK65878.1"
                     /translation="VQLEMIRTIPGLEEVQVMRPAYAVEYDYVPPVELYHTLETKRVK
                     GLYHAGQINGTSGYEEAAAQGIIAAINAGNKLLGMPPLVLQRSEAYTGVLIDDLVNKG
                     ADEPYRMFTSRAEYRLLLRQDNADERLMRYGYEIGLIDKELYEKTVEKYKRIQELTDR
                     INTVSIVIDDKLQEILNNKQIAIEERSQIQAGKLLRRPEISLDDVLPSIIQSDSSLVM
                     NEQEAKIVEMNIKYEGYIKKDLERIKKLEQMENKKIPETINYDEIIGLKNEAREKLKK
                     IRPETIGQALRISGVDPSDISIILVHLESLHRKQSG"
BASE COUNT          872 a          598 c          422 g         1125 t
ORIGIN      
        1 gcgatcccct ttaatgaata catatagatt gacccatctg catctttgag aacttgcatt
       61 ccttcattgc cataattaaa tattattgcc attatacctt ttgatacccg cactccatca
      121 attaaaacac catatgagtc ccatacaaca acaaatgata ctctgccatt atagaatcct
      181 atgattgtta aaggattccg gtatgtatat aaataatgaa aaggcaatga agcttcagat
      241 tgtattttta acacatcctt taaaaaatca tgtgttacat gttgtgtctt tttaaaagga
      301 ttccctttta ttaatagaac ttcctgttca gacatatcaa tcactgtaga tgatgcccca
      361 atgccaggta taatcagcgt ttcatctttc actatcatac tgtgcagttg tccttgaggt
      421 atttgagcat taattcctgt aagaggaata aaatacacta aaaacataca aagaagatat
      481 ttctttttca tatggttcat gagcttatat tccttaaact ctcaacttcc ctttttacaa
      541 cagcatgttt tcgtggatag tttgctggtg tatacccatc tttcaaaaat atcaaacctt
      601 catatagttt aagctttttg gcttcttcta gggttaccct atgaccgcca agattaagag
      661 aatgcttcgt taaatagtcc gttaatactg ttatatcatg tttattggca tttttttctt
      721 catgcaattt atcacatcca tactctgcta ttacttcatc attactgcat accgttttat
      781 tgttttcaaa acctttttca tacagattaa aggaataata tatatacagc catcctcccg
      841 gctttacaaa aggcaatcca tactcaagtg ccataagcgg gtgagcaaaa gcacgtgcca
      901 cagcaaaatc aaagctatcc ctgtaggcct tttcttttgc tatctcctct accctgccct
      961 gaattaccgt aacgatgtca tttataccca atgtatttat tgcattcagc aggaatttaa
     1021 tttttttatt atttgaatct accagaacgc cttttatatt gccaccaaaa tacagcaaaa
     1081 gtggaattcc aggtatcccc gcaccagtgc caatatctat aaacgatgtt ccacgtggaa
     1141 cattcacatg cttcaaaggc gttatgcttc ccagaataag ttcgttgtaa atggattctg
     1201 tatcctttaa tccagtaata ttagtcgttt tattatgttc atatataagt cgtgcatatt
     1261 ccttcagctt tctgtttatt tcatcaggta tgattactgt atttgaaatt gtcatactta
     1321 ctattctctt gtatctagat ttagaaataa ttatggattt gttataatag aatttattcc
     1381 ttacttcttt gcaacgatct tttcaattaa aaacaataat tattttcata cttccattct
     1441 ttttttcaat atctatttca tatctgtttt ctatatgtat caatcaacct gtatattgta
     1501 ttattatgta aaatacataa acatattaat tatatactgt ttattttcat ataaatgcaa
     1561 ttaccttgag gaataatggt ttttttattt aatcaatatt tttgcagcca ttaatagctt
     1621 tttatgagat agtatatatt tataccaatc cttttctcat gtttttaatc attacttcac
     1681 acaatattat actattcaac tatttgatta aattttattt tcgggtttgc aagcaccttg
     1741 tcgagcgttg gcagaatcat tttgatattt acattgcagt gacactctat catcatgtaa
     1801 tgggtttgtg agtaaactga agtaaaggca aagcagaaca tctggcatag atatccttta
     1861 ataattagat attgataatc ctcatcacga tttgcaattg aatatgtaag aataataatg
     1921 tagaatattt ctcaataacc ttttttatac agccttaatt tcagtattat atttattcgt
     1981 tatctttcag gggattttaa tgtgacataa tgttccacgt ggaacattca atctcaggaa
     2041 tcatattata aaccatatat gtacaaaatt agcaacttaa ccggattgtt ttctgtgtaa
     2101 tgattcaaga tgtaccagaa ttattgatat atctgaagga tcaacgcctg aaatacgaag
     2161 cgcctgccct atggtttcag gccttatttt tttcagcttt tcccgtgctt cattcttaag
     2221 cccaatgatt tcatcataat tgatggtttc gggtattttt ttgttctcca tctgttccag
     2281 cttttttata cgctccaggt ccttctttat atagccttca tactttatgt tcatctcaac
     2341 aatctttgct tcctgttcat tcattacaag gctactatcg ctttgtataa tagatggcaa
     2401 cacatcatca agactgattt ctggtcttcg caaaagtttt ccggcttgta tctggcttcg
     2461 ttcttctatt gcaatctgtt tattattaag gatttcctgt aacttatcat caataacaat
     2521 agaaactgta ttaattctat ctgtgagctc ctgaattcgt ttatactttt ccactgtctt
     2581 ttcatataat tctttatcta tcaggccaat ttcatatcca tagcgcatta accgttcatc
     2641 agcattatcc tgcctcagga gcaacctgta ttcagcccgt gaagtaaaca tcctgtatgg
     2701 ttcatctgca cctttattaa ccagatcatc tataaggacg ccagtatatg cttctgaacg
     2761 ctgtaacaca agcggtggca ttcctaacag cttattgcct gcatttattg cggcaataat
     2821 accctgtgca gcagcttctt catatcctga tgttccattt atttgccctg catggtataa
     2881 gcctttgact ctttttgttt ccagtgtgtg gtacaactct acaggaggca cataatcgta
     2941 ttcaacagca tatgcaggcc gcataacctg cacttcttcc agccctggta tggttcttat
     3001 catctctaac tgaacat
//
LOCUS       DAOSZP010000256         3014 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_25631_length_3014_cov_4.161541, whole genome shotgun sequence.
ACCESSION   DAOSZP010000256 DAOSZP010000000
VERSION     DAOSZP010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3014
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25631_length_3014_cov_4.161541"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1045)
                     /locus_tag="PK348_06405"
     CDS             complement(<1..1045)
                     /locus_tag="PK348_06405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65879.1"
                     /translation="MKKIFIFILLIFISCGQLIKQSPQKDYERIYKNLTNAESLENPV
                     QKDKLINKAYTLLMQRQAELIKEKKSDTTVAALLAYYYYLKGNYQDASSQVELASSPS
                     GNNQLGAIIQARVLLSTKGKAGAQGALDILRAVEGLDNPMAFIAMGDAYFLKADYDKA
                     RDNYTKALLLNRELQVVAANRLEVISRIKTLSINVAKVSDFILEPTMTRDKLAYILYE
                     IFELQKYIATAKPLDANFIDLEKSQYKSAIVSLRQKGFFSYIQGSAFEPFMVVSRGEM
                     AKLVEDFLVLSRNNEGLRSKFKNESPPFFNDIKNDNEYYNAMRLAVDMGIMTASLSQD
                     AYPDESVTGLQAIM"
     gene            complement(1343..2011)
                     /locus_tag="PK348_06410"
     CDS             complement(1343..2011)
                     /locus_tag="PK348_06410"
                     /inference="COORDINATES: protein motif:HMM:NF019559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HXK65880.1"
                     /translation="MPDIIVQTNVAYLVGIFFIIFILREIVAFKKILKLKYIFTPLIT
                     YIVIIIALYLSYVRGFTVYNIFIIAGLVFALIADTFLMIEEISFFIHGLFFFLLTHLC
                     YVYALSINYHFGTIDIIIIMIILSIFFLYYLVIHKAKGKVYIPVILYMLVLSLVLLLA
                     IGTFLKNVYPRGAIVTMAAILFAISDGVLAFNQFVHKVPHSTVVTWSLYAPAQLLFAV
                     SCYY"
     gene            complement(2158..2424)
                     /gene="csrA"
                     /locus_tag="PK348_06415"
     CDS             complement(2158..2424)
                     /gene="csrA"
                     /locus_tag="PK348_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000960597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HXK65881.1"
                     /translation="MLVLARKVNESIMIGDDIEIVIIDIKGDQVKLGIKAPKSVAVHR
                     KEIYEEIQKENIAAMKSQLKPETLKDITDILQTHKEKNIDNQKK"
     gene            complement(2429..2881)
                     /gene="fliW"
                     /locus_tag="PK348_06420"
     CDS             complement(2429..2881)
                     /gene="fliW"
                     /locus_tag="PK348_06420"
                     /inference="COORDINATES: protein motif:HMM:NF009793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HXK65882.1"
                     /translation="MVTIATKPFGQIEVDERQIIDFPEGIYGFEDIKKFIILDANEKS
                     PFKWLQAYDEPDLAFVIIRPIDFMMQYELNVMPEDLEDIGAKSPDEVIVFAIVTIPED
                     PSKMTANLQGPVLINPKTRRGKQAISLSDKYTVRHYILEEIKKAQVKR"
     gene            complement(2906..>3014)
                     /locus_tag="PK348_06425"
     CDS             complement(2906..>3014)
                     /locus_tag="PK348_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001223328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HXK65883.1"
                     /translation="FPETIMNFRWLESVHNYALAVGARSIKPTLMDFLR"
BASE COUNT          975 a          577 c          488 g          974 t
ORIGIN      
        1 tcattattgc ctgcaatcct gttactgatt catcaggata tgcatcctgg ctcaaggaag
       61 cagtcataat acccatatca acagccaaac gcatagcatt ataatattca ttatcatttt
      121 ttatatcatt gaaaaaagga ggtgattcat ttttaaattt tgaccgtaat ccttcattat
      181 tacgtgatag tactaaaaaa tcctcaacta gctttgccat ctcaccacga gatactacca
      241 taaatggttc aaatgcgctc ccctgaatat acgaaaagaa tcccttctgt cgtaaagaga
      301 caattgcact tttatactga gacttttcca gatcaataaa attggcatca agtggctttg
      361 cagtggcaat atacttttgc aattcaaata tttcatataa tatatatgca agcttatctc
      421 ttgtcattgt gggctcaaga ataaagtcag aaacttttgc tacattgatg gacagtgtct
      481 ttatacggga tataacttca agtcggttgg cagcaacaac ctgcaattcc ctgttaagca
      541 ataaggcttt tgtgtaatta tcacgtgcct tatcataatc tgcttttaaa aaatacgcat
      601 cacccattgc tataaatgcc attggattat ccaagccttc cactgcacgt aagatatcaa
      661 gtgcaccctg agcacccgct ttcccttttg tagaaagcag tacacgtgcc tgtataattg
      721 cccccaattg attattcccg gatggagaag aggcaagttc aacctgtgat gatgcatcct
      781 gatagttccc ttttaaataa taatagtagg ccagtaaagc agctaccgtt gtatcagatt
      841 ttttttcttt tatcagttct gcctgccgtt gcatcaaaag agtatacgct ttgttaataa
      901 gcttatcttt ttgcacagga ttttccagtg attctgcatt ggttaaattt ttatatatac
      961 gttcataatc cttttgtggg ctctgcttta ttaattgccc gcaagaaata aaaattagta
     1021 gaataaaaat aaatatcttt ttcatatcac accaccatca tattatttac actatgctca
     1081 aagtattatc attaaattat actaaaaata actcattatt tacaagaaaa ataatgataa
     1141 tttttcacta attattccta aaatgtggaa atttgaataa aatataaaat agtgtacatt
     1201 cccgtcgcct tcggcggcgg gggtgtacac tattttcatt atgaaatttt ttaagaaatt
     1261 attgctgata attttttaaa tataaacaca atacaacatc acaccaccaa gatagtgcac
     1321 tgagttaata atcttttttt catcaataat agcaggagac tgcaaacaga agctgtgcag
     1381 gtgcatacaa tgaccatgta acaacagtgc tatgaggtac tttgtgtaca aactggttaa
     1441 atgcaagaac accgtcagat atggcaaaaa ggattgctgc catggttaca atggcacccc
     1501 gggggtatac atttttcaga aacgtaccaa tagccaggag caataccaag gataaaacaa
     1561 gcatatacag tataaccgga atatatactt taccttttgc tttgtgaatt actaaataat
     1621 acaaaaagaa aattgataga ataatcataa taataattat atcaatggtt ccaaaatgat
     1681 aattaatact cagcgcatac acatagcaca gatgggttaa taaaaagaaa aaaagtccat
     1741 ggataaaaaa gcttatctct tcaatcataa gaaaagtatc tgcaatcaat gcaaagacaa
     1801 gtccggcaat aataaagata ttatacacgg taaaaccacg gacatatgac agatacaatg
     1861 caataataat gacaatatag gtgataaggg gggtgaaaat atatttaagc ttcaaaatct
     1921 ttttaaaggc cactatctca cggagaataa aaataataaa aaatatacct acaagatatg
     1981 caacatttgt ctgcacaata atatcgggca ttttcccttc cccaaaaaat aggatttcac
     2041 caccatcatt cccaacatga tatggattca atcacgtgat tgtaagtcct gaatcatgtt
     2101 tgaatatgac aggatagtgt tatcatttac agcaggtact tatgaactgt gttactatta
     2161 ttttttttgg ttgtcaatgt ttttttcttt atgagtctgc aatatatcag taatatcttt
     2221 gagtgtttcg ggtttaagct gagatttcat tgcggcaata ttttccttct gtatttcttc
     2281 atatatctct ttacggtgca ctgccaccga ttttggtgct ttgattccta atttaacctg
     2341 atcacctttg atatcaataa taactatctc aatgtcatcg ccaatcatga tgctttcgtt
     2401 tactttacgc gccagtacca gcatgcaatc acctcttcac ctgtgctttt tttatctctt
     2461 ccaggatgta atggcgcacc gtgtatttat cgcttaatga tatggcctgt ttgccacgtc
     2521 gtgttttagg attaataagg acaggcccct gtagattggc ggtcattttt gaaggatctt
     2581 ctggtatggt aactatcgca aacactatga cttcatcagg gctttttgca cctatatcct
     2641 ctaaatcttc tggcataacg ttcagttcat actgcatcat aaaatctatg gggcgtataa
     2701 tcacaaatgc taaatcaggt tcatcatacg cctgcagcca tttaaatggt gatttttcat
     2761 tggcatccag aatgataaat tttttaatgt cctcaaaacc ataaatgcct tcagggaaat
     2821 caataatctg ccgttcatca acttcaattt gcccaaatgg ttttgttgct attgttacca
     2881 acggtagcac ctcttcatag cgttattatc gcaggaaatc cataagtgtg ggcttgattg
     2941 accgcgcacc aactgccagc gcatagttgt gtactgattc aagccatcta aagttcatta
     3001 tggtttcagg aaaa
//
LOCUS       DAOSZP010000257         3003 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_25746_length_3003_cov_2.574966, whole genome shotgun sequence.
ACCESSION   DAOSZP010000257 DAOSZP010000000
VERSION     DAOSZP010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3003
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25746_length_3003_cov_2.574966"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..480
                     /locus_tag="PK348_06430"
     CDS             <1..480
                     /locus_tag="PK348_06430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HXK65884.1"
                     /translation="VLYNSSYVVNDIAAGSCVVKPSTFDFFTLKKHLPAIFIAAPILK
                     KINSYEVPFLERFKWLIEWWDPNCRQTYYTYGGGWNEVVEAPEGVVVNALTASPPNEN
                     LLPNQSMLNGSLSTKLEVGDFVFYRPKQGDAIFQFRDIIVIRNGKIIASWKPFPERF"
     gene            652..1560
                     /gene="pstC"
                     /locus_tag="PK348_06435"
     CDS             652..1560
                     /gene="pstC"
                     /locus_tag="PK348_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014958593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HXK65885.1"
                     /translation="MLKKTLQSALNIHNVSDRMVKYIFAVVGFLSVSFLFLIMIFLFK
                     EGLPIFKVVSIKDFVFGTSWYPTSEPPKFGTLPLIVASLSVTFIGAIVAVPFSLGIAI
                     YLSELAPSFVREIVKPAIELIASIPSVIIGFFGMVVVAPFLQDHFDIDTGLNLFNASI
                     MLAFMAIPTIASISEDALSSVPVSLKEASYALGANRWQTIVHITMPAALSGIWTAIIL
                     GIARVLGETMVVLMVAGGATTFPTSLFDPVRPLTANIAAEMAEASYRSDHYFALFAIG
                     IILFILTMLFNVIANYLSMKYKFKYH"
     gene            1571..2437
                     /gene="pstA"
                     /locus_tag="PK348_06440"
     CDS             1571..2437
                     /gene="pstA"
                     /locus_tag="PK348_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HXK65886.1"
                     /translation="MASLQAKAKAIEKTSFIIISALSYTILVFLLFVLGYIFVQGYRA
                     LSWEFLTAFPKNEMREGGIFPALLGTLYLMIGSSVISVPIGVMTAIYLTEYVENSKVL
                     QFIRLGINNLAGVPSVVFGLFGLSVFVIFLDFGSSIIAGSLTLGILNLPLVIRSTEEA
                     LLTVPTSYREASLSLGATKWQTIYKIVLPTALPGILTGIMLSLGRAAGETAPIMFTCA
                     TFYTPHIPDSLFSEVMALPYHIYVLAIAGTHIEATRHIQYGTAIVLIALVLGLNFTGM
                     VLRYKYRKKFKQ"
     gene            complement(2447..2905)
                     /locus_tag="PK348_06445"
     CDS             complement(2447..2905)
                     /locus_tag="PK348_06445"
                     /inference="COORDINATES: protein motif:HMM:NF012350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65887.1"
                     /translation="MFELSNACFQGSNVISGSARGLVVNTGTRTYFGSISERLIEKRP
                     LTSFDKGVKSFTCLMIRFMVIMVLLVFMIVGMTKGNWIEALLFELSVAAGLTQNKELL
                     DILDNANNINDLLFNNNAFALSNWRILKKSSTCYLGERTCFDCIAIKLKE"
BASE COUNT          840 a          564 c          590 g         1009 t
ORIGIN      
        1 gtgctttata atagcagtta tgttgtaaat gatatagcag caggatcatg tgtggttaaa
       61 ccatctactt ttgatttttt tactcttaag aaacatttgc cagcaatatt tattgcagct
      121 ccaattttga aaaaaattaa tagttatgag gtaccatttc ttgaacgatt taagtggcta
      181 atagagtggt gggaccccaa ctgtaggcaa acatattata cgtatggagg tggatggaat
      241 gaagttgttg aagctcctga aggtgtagtg gttaatgcac tgactgcaag ccctcccaac
      301 gaaaatctgc ttcccaatca gagtatgctg aatgggtcac tgtcaacaaa acttgaagtg
      361 ggggatttcg tcttttacag gccaaaacag ggggatgcga tattccagtt tcgggatatt
      421 atcgtgatca ggaatgggaa aataattgca tcatggaaac cattccccga aaggttttaa
      481 ttttacaata tctttacagg gtcttcataa tttctttata attatgagat ttgtttcagt
      541 taattattat cagttactga aatacatttt ttctatttta tgtctgttat gttgagatag
      601 tactataaag tgaattaaaa aaatttataa atcaaataat agtaaattgc tatgttaaaa
      661 aaaacattac aatctgccct taatattcac aatgtaagtg acaggatggt aaaatatatt
      721 tttgctgttg ttggctttct ttcagtttca tttttatttt taatcatgat ttttctcttt
      781 aaagagggtt taccaatttt taaggtagtc agtataaagg actttgtttt tgggacaagc
      841 tggtatccaa catcggaacc gccaaaattt gggacattgc cacttattgt agcatcttta
      901 tccgttacgt ttattggagc aattgttgct gttccattca gtttgggtat tgcaatttat
      961 ctgtcagaat tagctccatc atttgttcgt gaaattgtta agccggcaat tgaacttatt
     1021 gcatccattc cttcggttat cattggcttt tttggtatgg tggttgttgc accattttta
     1081 caggaccact ttgatataga tactggtctc aatcttttta atgcatcaat catgctggct
     1141 tttatggcaa taccaactat tgccagtata tccgaagatg cattgtcatc ggtaccggta
     1201 tcgctaaaag aagcatcgta cgcgctggga gcaaaccgct ggcaaacgat tgtccacata
     1261 accatgccgg cagcgctttc cggtatatgg acggctatca ttttggggat tgcacgtgtc
     1321 cttggcgaaa ccatggtggt gctgatggtt gctggtggtg caactacgtt tcccacctcg
     1381 ctctttgacc ctgtgcggcc gttaactgcc aatatagcag ccgagatggc tgaagcatca
     1441 tatcgcagcg accattattt tgccctcttt gcaataggca tcattttatt tatactgacc
     1501 atgcttttta atgttattgc taactatctt tcaatgaaat ataaattcaa gtatcattaa
     1561 aaagggaact atggcatcct tacaggcaaa ggcaaaagca attgaaaaaa cctcttttat
     1621 aatcatcagt gccctgtcat ataccatact agtattttta ttgtttgtgt tgggatatat
     1681 atttgtgcag ggttaccgtg cattgagctg ggaattttta actgcatttc ccaaaaacga
     1741 aatgcgtgag ggggggatat tcccggcact tctgggcaca ttgtatctta tgattggttc
     1801 atcggttata tctgtaccta ttggcgttat gactgcaatc tatttaacgg agtatgtaga
     1861 aaatagtaaa gtgctgcaat ttattcgtct tggtatcaat aatctggctg gtgttccgtc
     1921 agttgtgttt gggctttttg gattgagtgt atttgtaatt ttcttagatt ttggttcatc
     1981 tatcattgct ggctcactga cactggggat cttaaatttg ccactggtca tacgctcaac
     2041 tgaagaagca ctgctcactg ttcccacatc ataccgcgag gcatcgcttt cgttaggggc
     2101 aaccaaatgg caaactattt ataagatagt cctgcctact gcattgccgg gcatattgac
     2161 cggtattatg ctgtcactgg gtagagcagc aggggagaca gctccgataa tgtttacctg
     2221 tgcaaccttc tataccccgc atatacccga ttcactcttt agcgaggtta tggcactgcc
     2281 atatcacatc tatgtgctgg caatagctgg cacccatatt gaggcaacgc gccatataca
     2341 atatggtacg gccattgtgc ttattgcact ggtactgggc ttgaatttca ccggcatggt
     2401 tttgcggtat aaatacagga agaaatttaa acagtaattc acacatttat tctttaagtt
     2461 ttattgcaat acaatcaaaa catgttcttt cgcctaaata acaagtagag cttttcttta
     2521 atattctcca attacttaag gcaaaagcat tgttattgaa tagcaaatca ttaatattat
     2581 ttgcgttatc caagatatca agaagttctt tattttgtgt taatccagca gctactgaaa
     2641 gctcaaatag caaagcttca atccagttac ccttagtcat gccaacaatc ataaagacca
     2701 gcaacaccat aattaccata aatctgatca tcaggcaagt aaaggattta acacctttat
     2761 caaaactggt gagtggccgt ttctctatta atcgttcaga tattgaacca aaatatgtcc
     2821 ttgttccggt atttacaaca agccctctgg cactgccact tatgacgttg cttccctgaa
     2881 aacaggcatt ggataattca aacacatagt tcctctcact tttctgtgga tcggcatttt
     2941 ttttcaatgg gcattgattc gccggttagt attgattggc tgacaaaaaa atcttttgca
     3001 ctt
//
LOCUS       DAOSZP010000258         2993 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_25834_length_2993_cov_3.348877, whole genome shotgun sequence.
ACCESSION   DAOSZP010000258 DAOSZP010000000
VERSION     DAOSZP010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2993
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25834_length_2993_cov_3.348877"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="PK348_06450"
     CDS             <1..246
                     /locus_tag="PK348_06450"
                     /inference="COORDINATES: protein motif:HMM:NF014003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HXK65888.1"
                     /translation="DRFGNCILSLPNEQYSLQLNAIIVNNKRYTLYHCKTYADVPLEA
                     IGILKGSSGFIEICMNQKDASKRLAVTTGDMVELFYE"
     gene            239..1450
                     /locus_tag="PK348_06455"
     CDS             239..1450
                     /locus_tag="PK348_06455"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012470511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cofactor-independent phosphoglycerate mutase"
                     /protein_id="HXK65889.1"
                     /translation="MSERKIAILIGDGMADYPIEKLGGKTPLQYAKTDHMDYLAKNGI
                     CGLARTVPSGMPPGSDVANLSVFGYDPATCYTGRAPLEALNMGINLGPDDVAFRCNMV
                     TIEHDIMNDFAAGHIDSEFSKIVIDEIKNTIHRDGVEFYAGVSYRNIMVVRNFKGELP
                     ITTPPHDIQGKKIHEYLPKGANSELLNDIMQKAAGAIAHSKKIADAKLKFKGNPTGLW
                     LWGGGFRPKMEPLSTRFGLHGHTIAAVDLIHGIGRAAGLTPIHVEGATGYIDTNYSGK
                     AQACIDAMKNANFVFLHVEAPDESGHEGNLEHKLKSIEDFDAKVVGPVVNKLNRYGNF
                     TVLVMPDHPTPLSLRTHSSDPVPFVIYAPDMNYRFNCKGFNEVDAGNTGIFVEHAHEL
                     LAMIVNNVYPW"
     gene            1458..2888
                     /locus_tag="PK348_06460"
     CDS             1458..2888
                     /locus_tag="PK348_06460"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HXK65890.1"
                     /translation="MSKKFIALVVIGVIIAGVVIYRAMAPATIDNAIEYIDSGNYKKA
                     LKVLNTLAKTSGYEDGERIYYYRLKALLGFINELNEDYADECKEIQKTKNKKIESKLK
                     AKLEEINKETEADLILVTDPECYISMGGTLYDEFVSLYPGSRYIESIDFDMLQFIMSK
                     HPGTINPVIEFYKRYPETTYLSSLVNMVLKALGNPKIELKGHAEKLTEMIQNFCNKYH
                     ASAEYYRLFRCKGENVNLRDSAGTHGQITGKLNKGEFVIQLERSMDSVQIGDVRDYWY
                     RVVSVSGSRGWVFGKFLERIEPMTKNLVSEDEGFTIDEHFSEWIDSHTPKGWQHMYPG
                     FEEAISFTKRKDIHIVKLDSGEKGGLYKKTGSFQNMVCKVKGRLLSGNVILAGVVGYG
                     GLAAVITLLPEEVDVCGYKIPYTTTQWHEYELRSEGKTLSLFIDGELVARKINTKKHD
                     LLKSSGIYILVCGTKTQAEIEYIRIK"
BASE COUNT          970 a          488 c          680 g          855 t
ORIGIN      
        1 gacaggtttg gcaattgcat tttatcattg ccaaatgaac agtattcttt acaattaaat
       61 gcaataatcg ttaataataa gcgctatact ttataccatt gtaaaacgta tgctgatgtg
      121 ccattagagg caattggtat attaaaaggt agtagcggat ttattgaaat ttgtatgaat
      181 caaaaagatg caagcaaacg tttagctgta acaacgggag atatggtgga attattttat
      241 gagtgaacga aaaattgcaa tactgattgg cgatggcatg gctgattacc caatcgaaaa
      301 gttaggaggt aaaacgccat tacaatatgc taaaaccgat cacatggatt atctggcaaa
      361 aaatggaata tgcggtcttg ctcgcacggt accttcaggc atgccaccgg gaagtgatgt
      421 tgcaaacctt tccgtgtttg gctatgaccc tgctacctgt tataccggca gagcaccgct
      481 tgaggcactc aatatgggca ttaatttagg acctgatgat gttgcgttcc ggtgtaacat
      541 ggtaactatc gaacatgata tcatgaacga ttttgctgca ggtcacattg attcagaatt
      601 ttcaaagatt gtcattgatg aaataaaaaa tacaatacac agggatggcg ttgaatttta
      661 tgcaggtgta tcatatcgca atattatggt ggtgcgtaat tttaaaggtg aactaccaat
      721 taccacgcca ccccatgata tacagggaaa aaaaattcat gaatacctgc ctaaaggtgc
      781 aaacagtgaa ttgctaaatg acatcatgca aaaagcagca ggggcgatag ctcatagtaa
      841 aaaaattgca gatgcaaaac taaagtttaa gggcaatcca acggggttat ggctgtgggg
      901 aggtggcttc aggccaaaga tggagccact ctcaacacgg tttggattac acggacatac
      961 tattgcagcg gttgacttaa tacatggtat tggaagggca gcgggactta ccccaattca
     1021 tgttgaaggt gcaacaggct atattgatac aaactacagc ggtaaagcac aggcttgtat
     1081 tgatgcaatg aagaatgcaa attttgtgtt tttgcatgtt gaagcacctg acgaatcggg
     1141 gcatgaaggt aatttagaac ataaacttaa atcgatagag gattttgatg ctaaagttgt
     1201 gggtcctgtt gtaaataagc tcaatcgcta cggcaatttc acagtgctgg tgatgcctga
     1261 tcatccaaca ccgctaagct tgcgtacaca ttcaagcgat cctgttcctt ttgttatata
     1321 tgcccctgac atgaactatc gctttaattg taaaggcttt aatgaagtag atgcagggaa
     1381 tactggtata tttgttgaac atgcacatga acttttagca atgattgtga acaatgttta
     1441 tccatggtga ggaatatatg tcaaaaaaat ttatagctct tgtagttatt ggtgtgatta
     1501 tagctggcgt cgttatatat agagctatgg caccggcaac cattgacaat gctattgagt
     1561 acattgacag cgggaattat aagaaggcgt taaaggtgct taatacactt gcaaaaactt
     1621 caggttatga agatggtgaa cgcatatatt attaccgttt aaaagcgctt ttaggattta
     1681 ttaatgaatt gaatgaggac tatgctgatg agtgcaaaga aatacaaaaa acaaaaaaca
     1741 aaaaaattga atctaagctc aaagctaaat tagaagaaat aaacaaagag acagaggcgg
     1801 atcttattct tgttactgat ccggagtgtt atatatccat gggtggcacg ttatatgacg
     1861 aatttgtgtc attgtatcct ggcagtcgct acatagaatc aatagacttt gatatgctgc
     1921 aatttattat gagcaaacat ccgggtacta tcaatccagt tattgagttc tataaacgct
     1981 atcctgaaac tacgtacctg tcatcgcttg taaatatggt attaaaagcg ctgggaaacc
     2041 caaaaattga attaaaaggg catgctgaaa agcttactga aatgattcag aacttctgca
     2101 ataagtatca cgcaagtgca gagtattacc ggttgttcag atgcaaaggt gaaaatgtca
     2161 atctacgtga ttcagctggt actcatgggc agattaccgg aaagcttaat aaaggtgaat
     2221 ttgttattca attagagcgt tcaatggatt cagtacagat tggcgatgtg cgtgactact
     2281 ggtacagggt agtttccgta agtggcagca ggggatgggt gtttggcaag tttttagagc
     2341 gcattgagcc tatgacaaaa aatttggtct cagaggatga aggttttact attgatgaac
     2401 attttagcga atggattgat tcgcatacgc caaaaggctg gcaacatatg tatcctggct
     2461 ttgaggaagc tataagtttt accaaacgta aagatataca tattgttaag ctcgattcag
     2521 gtgaaaaggg tggtctttac aaaaaaactg gtagctttca gaatatggtg tgtaaggtaa
     2581 aagggcgctt gcttagtggg aatgttattc ttgctggagt tgttggctac ggagggcttg
     2641 ctgcggtgat aacattgttg cctgaagagg ttgatgtgtg cggatacaaa atcccgtata
     2701 caacgactca atggcatgag tatgaattgc gtagtgaagg caaaacgctt tccttgttta
     2761 ttgatgggga acttgtggcg cgaaagatta atacaaaaaa acatgatctc ttgaaatctt
     2821 ctggcattta tattcttgtg tgcggcacaa agacacaagc tgagatagaa tatatcagga
     2881 taaaataata catttaaaca acaagttttt tgccaacaac acaccagacc atatctgcaa
     2941 tacttacagc cgatggtatc aggtagctga aattcaagaa tccatgtatc atc
//
LOCUS       DAOSZP010000259         2988 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_25867_length_2988_cov_3.118650, whole genome shotgun sequence.
ACCESSION   DAOSZP010000259 DAOSZP010000000
VERSION     DAOSZP010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2988)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2988)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2988
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25867_length_2988_cov_3.118650"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2058)
                     /locus_tag="PK348_06465"
     CDS             complement(<1..2058)
                     /locus_tag="PK348_06465"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HXK65891.1"
                     /translation="MDVNLFDWMKKHKFFTFVGIPVIVLTVILLYTSIVYIEWIHDKQ
                     KTLDKLFKYKQLIDRTEELKEGYSYTYSEIDVSAKVVDIPTRIYDRNNEIIGEFFEQK
                     REIVPYSYFPKWLIKAVIASEDRDFYVHNGVSLKGILRAFAVNLIHLRVVQGGSTITQ
                     QLAKVLFTDMERNIKRKIYEIFCALEIEKHYDKQDILSMYLNYIYFGNGAYGVEAAAK
                     MFFGKSIIDCNETECAMIVATISNPLIYSPLNNLDVSLKKTRRILKSMVDAGFLKQQM
                     AEIQYKNFLKQWDVQFSKDNTAESSLIGSFIFSLYRINRAPFFNEYIRRILVNKFSEE
                     VVKKGGLSVYTTLDATKQDIALAALKKGVQLQREYHENKAKYIKNPQRALDEMEKAKN
                     IEGAFVSINPFTGEIIAYAGGYSFSYQNQMDHVQQIRRQPGSSFKPVVYVAAIQDKVI
                     TPSTVMDDIPMTFKGGYSPKNYDGIYSGKIIIREALKKSINVVAVQVLEKEGYSRIMS
                     IIQKALNLSSSELDSRFYKTLSLALGTYELSPLENGVLHSTIVNGGDFIIPYGIRYVK
                     DYNGNIVWNNEEEVLLQIQEKRKEYGKIIDPVACAVVVSMLKSVFEEGGTASWVFKNK
                     SVPVDVAGKTGTSSDYVDAWFVGYTPDSVTAVWIGNKTGAISLGKGRSGGVVAAPVWA
                     DYVF"
     gene            complement(2079..>2988)
                     /gene="rlmN"
                     /locus_tag="PK348_06470"
     CDS             complement(2079..>2988)
                     /gene="rlmN"
                     /locus_tag="PK348_06470"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0002935 - tRNA
                     (adenine-C2-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0070040 - rRNA
                     (adenine-C2-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HXK65892.1"
                     /translation="NFSKDLRYHLDEQYEISPLKLVDRKKSVVDGTEKFLFKTFDGHF
                     IESVFIRNDGTDEGRLTICVSSQAGCPMGCAFCETAKIGFKRNLQTAEILDQINQVRR
                     LTGLKNNNVVFMGMGEPFLNYDNVLKAADIMNYTFGFHISVRKITISTCGMYEGIERF
                     IDEKRPYNLAISLNDTDMVKRAKNMPVEKKYPMEKIIRLLENKFPASRNRVTIEYIMR
                     KDNISEEDAQRLKTMFRYNRIKLNLIPLNKGKHNLEIPTQEEINKFIKRLEIMNIPIS
                     IRKSAGSDIDGACGQLSGKHYSSNSN"
BASE COUNT          912 a          610 c          433 g         1033 t
ORIGIN      
        1 aaatacataa tcagcccaaa caggtgctgc tacaacacca ccacttcttc ccttgcccag
       61 agagattgca cccgttttat tgccaatcca cactgctgta acagaatcag gagtataccc
      121 cacaaaccac gcatcaacgt agtcactact tgtacctgtt ttgcctgcaa catcaactgg
      181 aactgattta tttttaaata cccatgaagc agttccgcct tcttcaaata cacttttaag
      241 cattgaaacc acaactgcac atgcaacagg gtcaattatc ttaccatatt ctttgcgctt
      301 ttcctgtatc tgcaataaaa cctcttcttc attattccag acaatattcc cattataatc
      361 tttaacatac cgtataccat agggaataat aaaatcgccg ccattaacaa ttgttgaatg
      421 caatacacca ttttcaaggg gcgatagttc atatgtaccc agcgccaatg acaatgtttt
      481 ataaaaacga gaatccagtt cactgctgga caaattaagg gctttttgta tgattgacat
      541 tatacgggaa tatccctctt tttcaaggac ctgaactgca acaacattga tagatttctt
      601 caatgcttct cgtatgatta tctttcctga atatattcca tcataatttt taggggaata
      661 tccaccctta aatgtcatgg ggatatcatc cataactgtt gaaggcgtta taaccttatc
      721 ctgaatagcc gcaacgtaca ctactggctt gaacgatgaa ccgggttgtc ttcgtatttg
      781 ctgaacatgg tccatctggt tttgataaga aaagctatag ccaccagcat aggctataat
      841 ttctccggta aatggattaa ttgaaacaaa ggctccttca atatttttag ctttttccat
      901 ttcatcaagt gcacgctgtg gattttttat atattttgct ttattttcgt gatactctct
      961 ttgtaattgt acgccttttt ttaacgcagc caatgcaata tcctgttttg tagcatccag
     1021 ggtagtatat acacttaaac cacccttttt aacaacctct tcagaaaatt tattaaccag
     1081 tattcttcgt atatattcat tgaaaaatgg cgccctattg atacgatata agctaaatat
     1141 gaagcttcct attaacgatg attccgcagt attatccttt gaaaattgaa catcccattg
     1201 ttttaaaaaa tttttatact gaatttcggc catctgttgt tttaaaaagc ctgcatcaac
     1261 catcgatttc aaaatccttc ttgttttctt tagtgaaaca tcaaggttat taagtggtga
     1321 ataaataagt ggatttgaga tagtagcaac gatcattgca cattcagttt cgttacaatc
     1381 tatgattgac ttaccaaaaa acatttttgc agcagcttca acaccatacg caccattacc
     1441 aaaatatata taattaaggt acattgatag gatgtcctgc ttgtcataat gtttttctat
     1501 ttccagtgca caaaatattt catatatttt gcgctttata ttacgctcca tatcagtaaa
     1561 cagcactttt gccagttgtt gcgtgatagt gctgcccccc tgtacaactc tcagatgaat
     1621 cagattgaca gcaaatgccc gtaaaatgcc ttttaaacta acaccattat gaacataaaa
     1681 atctctgtct tcactggcaa taactgcttt gataagccat tttggaaagt atgaataggg
     1741 aactatctcc cttttttgtt caaaaaattc gcctattatt tcgttattgc gatcataaat
     1801 tctggtagga atatctacaa cttttgcaga aacatctatc tcactatacg tatatgaata
     1861 accctccttc aattcctctg tgcgatcaat cagttgttta tatttgaaaa gcttatcaag
     1921 ggtcttttgt ttgtcatgta tccattcgat atatacaatt gatgtataca acagaatcac
     1981 agtaagaaca ataacaggaa tcccaacaaa tgtaaaaaat ttatgttttt tcatccaatc
     2041 aaatagatta acatccatac cataagcctt gtgatttttt agttactatt gcttgaataa
     2101 tgcttccctg aaagctggcc acaagcacca tctatatcac ttccggcact tttccttata
     2161 gaaatgggta tattcataat ttcaagtctt tttataaatt tatttatttc ttcctgggtg
     2221 ggaatttcta agttatgctt gcccttgttt aacggtataa gattaagttt aatacggtta
     2281 tatctgaaca tggtttttaa acgctgtgca tcttcttcag aaatattatc tttccgcatg
     2341 atatattcta ttgttactct atttcgcgat gcaggaaatt tattttccag taaccgtatg
     2401 attttttcca taggatattt tttttcaacc ggcatattct ttgcacgttt taccatatcg
     2461 gtatcattta atgatattgc taaattatat gggcgttttt catctataaa tcgttcaata
     2521 ccttcataca taccacatgt cgatattgta atttttctta ccgatatatg gaagccaaaa
     2581 gtataattca taatatcagc agcttttaat acattatcat aattaaggaa tggctctccc
     2641 attcccataa agacaacatt attatttttt aatcctgtta aacgccttac ctgatttatc
     2701 tggtccagaa tttctgcagt ttgtaaatta cgcttaaagc caattttagc tgtttcacaa
     2761 aaagcgcacc ccattgggca acctgcctgg cttgaaacgc agatggtcaa tcgtccttca
     2821 tcagtgccat cattacgaat aaaaacagat tcaataaaat gaccatcaaa ggttttaaat
     2881 aaaaattttt ctgtaccatc aaccactgat tttttacgat caaccagttt tagcggtgat
     2941 atttcatact gttcatccag atggtagcgc agatccttgg aaaagttt
//
LOCUS       DAOSZP010000260         2986 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_25886_length_2986_cov_3.357898, whole genome shotgun sequence.
ACCESSION   DAOSZP010000260 DAOSZP010000000
VERSION     DAOSZP010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2986)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2986)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2986
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25886_length_2986_cov_3.357898"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /locus_tag="PK348_06475"
     CDS             complement(<1..478)
                     /locus_tag="PK348_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HXK65893.1"
                     /translation="MCGIVGYIGNKNVAEVLINGLRRLEYRGYDSVGIAALNNKLIIQ
                     KQKGKVSELNAILNYDDFKGFSAGIGHTRWATHGEPNDTNAHPHTDCSGEIALVHNGI
                     IENYAAIKKILLQHNHTIKSETDTEVLVHLIEYYHQTMPLVDAVQKTLTKVEGTYGI"
     gene            complement(907..1077)
                     /locus_tag="PK348_06480"
     CDS             complement(907..1077)
                     /locus_tag="PK348_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009247091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HXK65894.1"
                     /translation="MAYKITEECTMCGTCAAECPSQAISEGKDRYVIDQDACTECGTC
                     VDVCPAEAIVEV"
     gene            1465..2457
                     /locus_tag="PK348_06485"
     CDS             1465..2457
                     /locus_tag="PK348_06485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65895.1"
                     /translation="MKYISKISIVLLLVFTCSAVFAGQWVYKPMSINAQKGDIILSTS
                     PGFIMDLLAILGCYWSHSGMAVDNGFNIRHNTMYVSEVPIEYNYIWFIKTTPKRMDPT
                     RLSNGLPGILTEDIDTTYNVTKNFHAAGGAVLKPTAANEALYRQYLQLAADKLLYVKA
                     YYRVNAYVNMYQLDYVNYYITGRGNHCSGTCWYANYFAGKTMNVAYIPPSLVTQCAYN
                     LYNSVKNMVRDEAGGFGAFIIDIEGLFGTGADEKIANQIVNTFGWDRSWDTSSYWRTY
                     INQKSATANAPDHLLLYTYTNPSGYNPGVQTTSSSYYGQVDPLVITSGYYYWVD"
     gene            2605..>2986
                     /locus_tag="PK348_06490"
     CDS             2605..>2986
                     /locus_tag="PK348_06490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65896.1"
                     /translation="MKRTYIITGIIIFCIAAFVLILSVNKSNQQDDTSSKTQSLLNVF
                     QFMSAPEPQEEIPPVDPEKVETYEIEQANFIKEIILEKNPVCAGEDFKVTVIAKNPFG
                     PDAHLVYRISNKLGNPAILRFTKAG"
BASE COUNT          955 a          545 c          492 g          994 t
ORIGIN      
        1 caatgccata tgtaccctca actttggtta atgtcttctg aactgcatct accagtggca
       61 ttgtttgatg atagtactca attaggtgta ccagaacctc ggtatcggtt tcacttttaa
      121 tagtatgatt atgctgtaaa agaatttttt taatggctgc ataattttct ataatgccat
      181 tgtgcactaa cgctatctca ccactgcaat cagtatgagg atgagcatta gtatcatttg
      241 gctcaccatg agtggcccat cgtgtgtgcc caatcccagc agaaaacccc ttaaaatcgt
      301 cataatttaa aatggcattg agctcagaaa ctttaccttt ttgcttttga ataatgagct
      361 tgttattgag tgcagcaata cctacagagt catacccccg gtattcaagg cggcgtaatc
      421 cattaataag aacttctgct acatttttat tgccaatata gcccacaatg ccacacataa
      481 aaaattttct ccttatgaaa gtagaatata attttaatgt cttttctaaa ccatagaggg
      541 taagaaaact tatttttatt acagtattgt cattttgaat gtgtccaaca aaatatgtgt
      601 gcgtgaagaa ttaataatta aattcttctt ccactttttc gggaacataa tatgctaagt
      661 tttctcacca cttcttagag tgtaatgata atgaataata taatcaccta aaaagtttta
      721 aacgatagta ttttctttaa agagctatac aacgatattg cacgaacata gttttttcat
      781 ggttacatat cgccacttac gttgctctta atgacttaat tataaactat ttttttatta
      841 aaagcaaaac attttttata tttgttattg attaacagca atggggcttt ttaggcccca
      901 ttggtattat acttcaacta ttgcttcagc gggacagaca tcaacacatg taccacattc
      961 tgtacaagca tcctggtcta taacatatct atctttgcct tcagaaattg cctgtgacgg
     1021 gcattcggcg gcacaggttc cacacattgt acattcttcg gtgattttat aagccatgta
     1081 tgtttcctcc tgaaaatata gcattaactt tacgtataac aattaaaata aattagtagc
     1141 acaattttta ttagaaacaa aatttataga gataataata ccaatattgt taagaatata
     1201 aataaattat cattgatagt acctgttaat tatgtcttcc tatcatttca atgaaattaa
     1261 aaatatatgt ttcttttaca attatatttt atatattttt gaaaaaaaat gcttatatat
     1321 accttttagt cggtttttaa attgactttt tcattggctt aatctcttta ttattatagt
     1381 gatatgttgt aattaaatga gtgattattc actcatcatg ccattgcctg tatatctaat
     1441 tttaattttt ataaaggggg ttctatgaaa tatatttcga aaattagtat tgtattatta
     1501 ctagttttta catgctctgc agtttttgca ggccaatggg tatacaaacc aatgagtatt
     1561 aatgcccaga aaggtgatat catattatca accagtccgg gttttattat ggaccttctg
     1621 gcaatactgg gttgttactg gagccacagc gggatggctg ttgacaatgg cttcaacatt
     1681 cgccataaca ccatgtatgt gagcgaagtt cccattgaat ataattatat ctggtttatt
     1741 aaaacaacac caaaacgtat ggatcctacc cgtttgagca atggattgcc aggaatactt
     1801 actgaagata ttgacacaac ttacaacgta acaaaaaatt tccatgctgc gggcggtgct
     1861 gtattaaaac caacagctgc aaatgaagca ttatacagac agtatttgca gttagcagcc
     1921 gataagttgc tgtacgttaa agcatattac cgggttaatg catatgtcaa tatgtaccag
     1981 ctcgattacg taaattatta tataacaggc cgtggtaacc actgctccgg tacatgctgg
     2041 tatgccaatt acttcgcagg caaaacaatg aatgttgcct atatcccacc ttcactggta
     2101 acccaatgtg cttacaatct ctataattca gtaaagaata tggtacgtga tgaagccggc
     2161 ggatttggtg cattcatcat agacattgaa ggcctctttg gcactggtgc tgatgaaaag
     2221 atagcaaatc agattgttaa cacgtttggc tgggacagat cctgggacac ttcttcatac
     2281 tggagaactt atataaacca gaaatccgca acagctaatg ctcctgatca cctgttgctg
     2341 tacacatata ccaatccttc tggatataat cctggtgtac aaactacctc ctcttcatac
     2401 tatggacagg ttgatccact ggttattacc tccggatact attactgggt ggattaactg
     2461 catgaacaca acaatcccgg gtgccatttt gcccgggatt tttttattga ttaaattaat
     2521 tctttattat gatctatcac aaattaaaat agtatagcta tttcataatc ttataacact
     2581 attgtaaaac acaggatacc atctatgaaa cgcacataca ttataaccgg aattattatt
     2641 ttttgtattg cagcatttgt attaatacta tctgttaata aaagcaatca acaggatgat
     2701 acttcttcaa aaacacaatc actgcttaat gtatttcaat tcatgtcagc acctgaacca
     2761 caagaagaaa taccaccggt tgaccctgaa aaggttgaaa catatgagat tgagcaggca
     2821 aatttcatta aagagataat actggaaaaa aatccagtat gtgcagggga agattttaaa
     2881 gttactgtta ttgcaaaaaa tccttttggc cctgatgcac atttagtgta tcgcatcagc
     2941 aacaagctgg gtaatcctgc aattttacgg tttaccaagg caggat
//
LOCUS       DAOSZP010000261         2974 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_26005_length_2974_cov_2.982528, whole genome shotgun sequence.
ACCESSION   DAOSZP010000261 DAOSZP010000000
VERSION     DAOSZP010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2974)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2974)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2974
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26005_length_2974_cov_2.982528"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            87..1631
                     /locus_tag="PK348_06495"
     CDS             87..1631
                     /locus_tag="PK348_06495"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXK65897.1"
                     /translation="MKLFILIFYGLILLLLSIFGAHRYYTLYLFLKYAKKKIQPKSMF
                     KSLPKVTVQLPIFNEQYVVERLINTITQLDYPKKKLEIQVLDDSTDETSIIAERICKL
                     KRKEGFNIKYIHRTDRTGFKAGALENGLGLTDSEFIAVFDADFLPNPDFLQKTIHYFT
                     DPEIGMVQTRWDHINRHYSLLTECQSILLDGHFMIEHTARNRSGRFFNFNGTAGIWRR
                     QAIIDGGGWQHDTITEDLDLSYRSQMKGWKFLYLPDVTVPAELPIEISAFKAQQFRWA
                     KGSIQVFKKLYKTILHSHLPLKVKLESFMHLGANFAYLLMVFLTLLMPVALWVRYYGG
                     FKKFAYLDLPIFLLATLSVMIFYYFTELIVLYQNKNIYDSKIKPLLYLPMVLSLGIGL
                     SINNSKAVIEALLDKKSDFNRTPKYNVVALNRINAIDHVKTLLKNKYSLHTINFVSII
                     EFVLGLYMTLAVYFAFDKNLYISIPLIMLFQVGYLYTSLFTIINSITHQVLMYISLFR
                     RGLANE"
     gene            complement(1640..2449)
                     /locus_tag="PK348_06500"
     CDS             complement(1640..2449)
                     /locus_tag="PK348_06500"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXK65898.1"
                     /translation="MAINFNNRWVVITGASSGIGKALAECFAREGAHLILGSLPGEKH
                     ILESIRDNLQKQHAIQIVTIYQDFADPDGAVKFYDQCKKLQKQIYCLVNNAGIMAYGY
                     FHELSHKQQKTLININLMAYFSLMRLFIEDMIQQEEGVIINISSVSAFQPTPHHAVYG
                     AVKAFVQSLSEAVDQECRGTGISVHTINPSYTDTPLLKKNNFPNKIWWYRISGLAKPE
                     DIARKAIKAVKQGKRLYVPGVMNALIHLFLPRLLPRKLSNAIAHIVLRQER"
     gene            complement(2468..>2974)
                     /locus_tag="PK348_06505"
     CDS             complement(2468..>2974)
                     /locus_tag="PK348_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015905789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HXK65899.1"
                     /translation="VCSEKFLKKLTGARPVKIIASVMGQGEDRDLDAPDIGERLSRLA
                     YEQAGVGPGDISLAELHDATSWGELHQTESMGFCPVGEGGPFAESGATTLGGKKPINT
                     SGGLECRGHPIGASGLAQIHELVLQLRGEAGARQVENARLGLAENGGGNIGVEEAAMC
                     IHILEKVR"
BASE COUNT          865 a          660 c          533 g          916 t
ORIGIN      
        1 aatccatttt caatattgta aatctgaaaa aatatcacat caaccatact gaaagatatt
       61 ttaataataa aaggaagata tactctatga agctgtttat tcttattttt tatggattaa
      121 tacttttact tttatcaatt tttggtgctc atagatacta tacattgtat ctttttctaa
      181 aatacgccaa aaagaaaatt caacctaaat caatgtttaa atctctcccc aaagtaactg
      241 ttcagcttcc tattttcaat gaacagtatg ttgttgagcg ccttatcaat actataacgc
      301 aattagatta cccaaaaaag aagcttgaaa tccaggtact tgatgactca accgacgaaa
      361 cctcaattat tgccgagcga atatgcaaac tcaaacgcaa ggaaggattc aatatcaaat
      421 atattcatcg aacggacaga acagggttta aagcaggtgc tctggaaaac ggccttgggc
      481 ttaccgatag cgaatttata gcagtctttg atgcagattt tttgcccaat cctgactttt
      541 tgcaaaaaac aatccattac tttaccgacc ctgaaattgg aatggtgcag acacgatggg
      601 atcatataaa tcgccattat tctttgctta ctgaatgtca gagcatactg ttagatggcc
      661 acttcatgat agagcatacc gcacgcaatc gctcaggccg cttctttaat ttcaatggca
      721 ctgctggcat ctggcgaagg caggcaatca ttgatggtgg tggatggcaa catgatacca
      781 taaccgagga tcttgactta agctaccgat cacagatgaa aggatggaag tttttatatc
      841 ttcccgatgt taccgtacct gcagaacttc ccattgagat ttcagcattt aaggctcagc
      901 aattcagatg ggcaaaggga tccattcagg tatttaaaaa attatataaa actatcctgc
      961 attcacacct gccccttaaa gttaagcttg aatcgttcat gcatttaggg gctaactttg
     1021 catatcttct catggttttt cttactcttc ttatgcctgt agctttatgg gttcgttatt
     1081 acggagggtt taaaaagttt gcctatcttg acctgccaat atttctcctt gccacactat
     1141 cagtaatgat tttctactat tttacagagc tcattgttct ttaccagaat aaaaacattt
     1201 acgattcaaa gattaaaccg cttctgtacc tgcccatggt cctttcactg ggtattggtt
     1261 tatccattaa taacagtaaa gcggtcatag aggcactgct tgataaaaag agtgatttta
     1321 acaggactcc aaaatacaat gtggtggctc tcaatcgtat taatgcaata gaccacgtga
     1381 aaacgctatt aaaaaacaaa tatagcctgc atacaatcaa ttttgtctct attattgagt
     1441 ttgtgttggg gctctatatg acgttggcgg tgtattttgc atttgataaa aatctatata
     1501 tatccattcc gctcatcatg ctgttccagg ttgggtatct gtacacatca ctgtttacca
     1561 tcatcaacag cataacacat caggtactca tgtatatcag tcttttccgc agaggcctgg
     1621 caaatgaata aagacaggat tatctttcct gccgtaaaac aatatgtgct attgcattgg
     1681 acagttttcg tggcagcaat cgcggcagga aaagatgaat caatgcattc atgaccccgg
     1741 gcacatataa tcttttacct tgctttactg ccttgattgc tttgcgagca atatcttcag
     1801 gttttgccag ccctgaaata cggtaccacc agattttatt cggaaaatta ttttttttaa
     1861 gcaagggcgt atccgtatag gatggattta ttgtatgcac gctgataccg gtacctctac
     1921 attcctgatc gacagcttcg cttaaactct gcacaaacgc tttaacagca ccatatacag
     1981 catggtgcgg agtcggctgg aatgctgaca cagatgatat attaataata accccttcct
     2041 cttgttgtat catgtcttca ataaatagtc gcatgaggga aaaatatgcc ataaggttaa
     2101 tattgattaa tgttttttgc tgtttgtgcg atagttcatg gaaatagcca taggccatta
     2161 taccggcatt attaactaaa cagtaaatct gcttttgtag ctttttacat tgatcataaa
     2221 attttacggc tccatccgga tctgcaaaat cctgatagat agtaactatt tgaatagcat
     2281 gttgcttttg aagattatcc ctgatgcttt caagtatgtg tttttcacca ggaagagaac
     2341 ctaatataag atgagcccct tccctggcaa agcattccgc cagtgcctta cctataccgg
     2401 aagaggctcc tgtgataacc acccacctgt tgttgaaatt aatagccatt gattacatta
     2461 tcagtaacta tcgcaccttt tctaagatgt gaatacacat agctgcttct tcaacgccta
     2521 tgttgccccc accattttcg gccagcccta accgggcgtt ttcaacctga cgcgctcctg
     2581 cctcaccgcg caattgcagt acaagttcat gaatctgggc aaggcctgat gcgcctatgg
     2641 gatgtcctct gcactccagt ccaccactgg tgttaatggg ctttttacca cccaacgttg
     2701 ttgcgccact ttcagcaaag ggaccgcctt caccaacagg gcaaaaaccc attgattcag
     2761 tctgatgcaa ctcaccccag ctcgttgcat catgcagttc agcaaggctt atatcgccag
     2821 gaccaacacc agcctgctca tacgctaacc ttgataaacg ttcacctata tccggcgcat
     2881 caaggtccct atcctcaccc tgtcccataa ccgatgctat aatttttacc ggacgcgctc
     2941 ctgttaattt ttttaaaaat ttctcagaac atac
//
LOCUS       DAOSZP010000262         2971 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_26040_length_2971_cov_3.318930, whole genome shotgun sequence.
ACCESSION   DAOSZP010000262 DAOSZP010000000
VERSION     DAOSZP010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2971)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2971)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2971
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26040_length_2971_cov_3.318930"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..449
                     /locus_tag="PK348_06510"
     CDS             <1..449
                     /locus_tag="PK348_06510"
                     /inference="COORDINATES: protein motif:HMM:NF018696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HXK65900.1"
                     /translation="GLYCYQGDIDEGLSDYKDNSFDYVIVNQTLQSTKKPDVVIRESL
                     RIALNAVFSFPNFAHYSLRLYLSLYGRMPKNKLLPYEWYETPNIHLLTIKDFRDYCRD
                     FKYPIKKEMHFSTIRSRSVIIPVMPNLFAEYGFFILDGQPFTSATL"
     gene            545..2551
                     /gene="uvrB"
                     /locus_tag="PK348_06515"
     CDS             545..2551
                     /gene="uvrB"
                     /locus_tag="PK348_06515"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HXK65901.1"
                     /translation="MPQFKVVSAYKPSGDQAQAIEKLAEGIASGYKYQTLLGVTGSGK
                     TYTMAKVIEKVQKPTLVLTHNKTLAAQLYREFKEFFPNNAVEYFVSYYDYYQPEAYVV
                     SQDLYIEKDASINDEIDRLRLKATSSLVEREDVIIVSSVSCIYGIGNPESFEKNHIRL
                     AVGDMVDRQELLMKLVSIHYDRNDIAFTRGTFRVRGDTVEVFPANLQDALRIVFFGDE
                     IEALYKINPVTGARQETLEKAYIYPAKHYVSSKEEMEETIRMIEIELEHRIKEFMDAG
                     KLVEAQRIEMRTRYDIEMLKTVGYCNGIENYSRIIDRRQPGQPPHCLLHYFKSNYLMF
                     IDESHVTIPQVRGMYEGDRSRKVNLVEYGFRLPSALDNRPLKFDEFEAMIDQVVFVSA
                     TPADYEIEKSDQVVEQVIRPTGLIDPDIEVRPATNQVDDLIGEIKKRVAANERVLVTT
                     LTKKMAEDLTEYLTEVGLKVRYLHSEIETIERVEIIRDLRKGEFDCLIGINLLREGLD
                     IPEVSLVAILDADKEGFLRSTRSLIQTAGRAARNINGKVIMYADTITRSMKEAIEETN
                     RRRALQMEFNKKHKITPRSISKEIVDIIEREYHSDDVFADIVAEYTQSYRTGNIEELK
                     KVRDKIKQDMLEAADNLEFEKAAILRDQLFDIEKKIGLYSKVAQ"
     gene            2573..>2971
                     /locus_tag="PK348_06520"
     CDS             2573..>2971
                     /locus_tag="PK348_06520"
                     /inference="COORDINATES: protein motif:HMM:NF014769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide pyrophosphorylase"
                     /protein_id="HXK65902.1"
                     /translation="MEIPSYDELSPLIEMALKEDIGSGDITSNAIFDEYHFTRARIYS
                     KSEGVFCGGFLIEYIYHTMDPSVSVTLYRNEGAPVKEDDVVADIEGNVRTLLSGERTV
                     LNFLQRTCGIATRTARMVSIVRDTNIKILDT"
BASE COUNT          952 a          527 c          661 g          831 t
ORIGIN      
        1 aagggctgta ttgttatcag ggtgacattg atgaaggatt gtcggactat aaagacaact
       61 cctttgatta tgttattgtt aatcagacat tgcagagtac aaagaaaccg gatgttgtta
      121 tccgtgaatc gctgaggatt gcactcaatg cagttttcag cttccccaac tttgctcatt
      181 actcattacg gttatacctg tcattgtatg gaaggatgcc aaaaaacaaa ctgttgccct
      241 atgaatggta tgaaacacct aatatacacc ttttaacaat aaaggacttt agagactact
      301 gcagggattt caaatatccc attaaaaaag aaatgcattt ttcaacaatt cgttcgcgtt
      361 cggttatcat accggtaatg cctaatcttt ttgcagaata cggtttcttt atacttgatg
      421 gacaaccctt tacttctgcc acactgtaaa atactaagat taatgctgca tggagtattt
      481 tcttgtattt ttactatggt atggctacat tgtatgtaaa ctcttacaaa accaaaggaa
      541 aactatgcct caatttaagg tggtatcagc gtataaacct tcaggtgacc aggcacaagc
      601 aatagaaaaa ttagcagaag gaattgccag cggttataaa taccagactc tgcttggagt
      661 taccggttca ggcaaaacat acaccatggc aaaggtgata gaaaaagtac agaaacccac
      721 attggtactt acccataata aaacacttgc agctcagctt taccgtgagt ttaaggaatt
      781 ttttcccaat aatgctgttg agtattttgt atcatattat gattactacc agcctgaagc
      841 gtacgttgtg tcacaggatc tgtatataga aaaagatgca agcattaatg atgagataga
      901 ccgcctacgt cttaaagcta catcatcact ggttgaacgg gaggatgtta tcatagtttc
      961 atccgtatca tgtatctatg gcattggaaa tcctgaaagc tttgaaaaaa atcatatacg
     1021 gcttgccgtg ggggatatgg ttgaccgcca ggagctttta atgaagctgg tgtcaatcca
     1081 ttatgaccgc aatgatatag catttacccg cggtacattc agggtgcgcg gtgatactgt
     1141 tgaggtattc cctgccaacc tgcaggatgc gttgcgtata gtgttttttg gtgatgagat
     1201 agaagcactg tacaaaatta accccgtgac tggagcacgc caggaaacgc ttgaaaaggc
     1261 atacatttat ccagcaaaac attatgtttc cagcaaagaa gaaatggaag aaaccatacg
     1321 catgattgaa attgagttag aacacaggat aaaggaattc atggatgcag gcaaacttgt
     1381 tgaagcacag cggatagaga tgcgtacacg atatgatatt gagatgctaa agaccgttgg
     1441 ctattgtaat ggcattgaaa attattcacg gattattgat cgcagacagc caggccaacc
     1501 accgcattgc ctgcttcatt attttaagag taactatctc atgtttattg atgagtccca
     1561 tgtaacaata ccacaggtac ggggtatgta tgaaggcgat agatcccgga aagtgaactt
     1621 agttgagtat ggtttcaggc tgccatcagc acttgataac aggccattga aatttgatga
     1681 atttgaagca atgatagatc aggtagtatt tgtttcagca acccctgctg actatgagat
     1741 agaaaaatca gatcaggtag ttgaacaggt tatacgacca acaggcttaa tagatcctga
     1801 cattgaggtc aggcctgcaa ccaatcaggt tgatgattta attggtgaga ttaaaaaacg
     1861 cgttgctgcc aatgaacggg tactggtcac aacattaact aaaaagatgg ctgaagacct
     1921 gaccgagtac ctgacggaag ttggattgaa agtgcgctat ctccattcag aaattgaaac
     1981 aattgagcgt gttgaaataa tacgcgattt gcgaaaagga gagtttgatt gccttattgg
     2041 gattaatctt ttacgtgaag gacttgatat acctgaggtg tcacttgttg ctatacttga
     2101 tgcggataag gaagggtttt tacgttcaac gcgttcgctc attcagactg ccggaagggc
     2161 tgcgcgtaat ataaatggaa aagtcattat gtatgccgac actattactc gttcaatgaa
     2221 ggaagcaata gaagaaacca acagacgacg tgcactccag atggagttca ataaaaaaca
     2281 taagattact cccaggagca tttccaaaga gatagttgac ataattgaac gtgaatatca
     2341 ttctgacgat gtttttgctg atatagttgc agaatatact cagagttatc ggactggcaa
     2401 tattgaagaa ttgaaaaagg ttcgagataa aataaagcag gatatgcttg aagcggctga
     2461 caatcttgaa tttgaaaaag cagcaatact tcgtgatcag ttatttgata ttgagaagaa
     2521 gattggactg tacagcaagg tggcacagtg acagtaatca gaggaatagt atatggaaat
     2581 accttcatac gatgaactat cgccacttat tgaaatggca ttgaaagaag acattggtag
     2641 tggagatatt accagtaatg caatttttga tgaataccat tttacccgtg cacggattta
     2701 ttccaagagt gaaggtgtct tttgcggagg atttttgatt gaatatatat accataccat
     2761 ggacccttcg gtatcggtaa cgctgtaccg caatgaaggg gcaccggtaa aggaagatga
     2821 tgttgttgcg gatattgaag gcaatgtacg gacgcttctt tcgggagagc gcacagtact
     2881 caatttttta cagcgaacct gcggaatagc cacacgaact gccagaatgg tttcgattgt
     2941 ccgtgataca aatataaaaa ttcttgatac c
//
LOCUS       DAOSZP010000263         2941 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_26358_length_2941_cov_3.727651, whole genome shotgun sequence.
ACCESSION   DAOSZP010000263 DAOSZP010000000
VERSION     DAOSZP010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2941
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26358_length_2941_cov_3.727651"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1589
                     /locus_tag="PK348_06525"
     CDS             <1..1589
                     /locus_tag="PK348_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXK65903.1"
                     /translation="KTAITEGLAQRIVEGTVPPVLKDAKIYRLDMGALIAGTRFRGDF
                     EERLKRVIAELQKKEKVILFIDEIHTIVGAGAVSGGSMDASNILKPVLADGKIRCIGT
                     TTYDEYRKYFEKDGALSRRFQKIDIHEPSHEDTIKILQGIKSRYEEHHRVTYTDEALQ
                     QAVELSARYITERKLPDKAIDVIDEAGAFARMYAHDDEEPIITAHDIERVIAKIARIP
                     ERTVSSSEIQKLQYLEDELKSQIFGQDGAVQKVVEAIKRSRAGFREAEKPIASFLFVG
                     PTGVGKTELARQLSLIMGIELIRFDMSEYQEKHTVARLIGAPPGYVGYEEGGQLTEAI
                     RKHPYCVLLLDEIEKAHPDVFNALLQIMDYATLTDNTGRKTDFRNTIIIMTSNAGVRE
                     MNKRVLGFEMATYNMDAVNKELERLFAPEFRNRLDAIVYFNGINKEMMRKIVIKKVNE
                     FAKQLEEKNVQLVCTDACIDWISEKAYSPVFGAREVARIIQEHIKSYFVDAVLFGSLK
                     NGGRAIADVVNDLVSITIEQ"
     gene            1591..2322
                     /gene="aat"
                     /locus_tag="PK348_06530"
     CDS             1591..2322
                     /gene="aat"
                     /locus_tag="PK348_06530"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012676692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HXK65904.1"
                     /translation="MLYYLDKDVIWFPPVEEAIEDGLLAFGGDLTPQWLLEAYSRGIF
                     PWFNSDNSEILWWSPDPRFILFPQELRLHRSLKQVMKKKIFTVSMDTAFQKVIAACAM
                     PRKKESGTWITARMQKAYIKLHAMGFAHSVEVWHDGMLAGGLYGVSLGNAFFGESMFT
                     SVDNASKVALVTLNAVARRLGFAFIDCQVYTDNLKRFGARFIERQEFMTLLNASLKHS
                     THQGSWSSLIHDGYDYLECLTGAGA"
     gene            complement(2303..>2941)
                     /locus_tag="PK348_06535"
     CDS             complement(2303..>2941)
                     /locus_tag="PK348_06535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65905.1"
                     /translation="PPDDRYAQEWEYFVLHPDDYLSRFESTIALIKASLKSIVISICI
                     PLLVMACIHITVTIALLVLFVFAIAIASYSSIIAGIIITALMIKRLAEIIAELFTQYV
                     KKGAAWMNEQLKGIPMKVGKMVFYGTFTSAFLLLLGYAGAVIASAYRIVAAPYARIFI
                     LIIGVIAVAVVSLSLYFMFSVFVTALMEYPKIRYTKIKAKKENLLESKLLLQ"
BASE COUNT          908 a          541 c          685 g          807 t
ORIGIN      
        1 gcaaaaccgc aattaccgaa gggcttgcac agaggatagt ggaaggcacc gtaccacctg
       61 ttcttaaaga tgcaaaaatt tatcggcttg atatgggtgc tcttatagcc ggcacacgat
      121 tccggggcga ttttgaagaa cgtcttaaac gggttattgc agaattacaa aaaaaagaaa
      181 aggttatcct ttttattgat gagatacata ccatagtggg tgctggagct gtcagtggtg
      241 gatcaatgga tgcttccaat atattaaagc cggtgcttgc tgatggcaaa atacgatgta
      301 ttggcacaac cacctatgat gaatacagaa agtactttga aaaagacggt gcattatcgc
      361 gacgtttcca gaagattgat attcatgagc catcgcatga agatacaata aaaatattgc
      421 aggggataaa atcgcggtac gaagagcatc acagggttac ctataccgat gaagcattac
      481 agcaggcggt ggaactttca gcacgctaca tcactgaacg aaagcttcct gataaagcca
      541 tagatgttat agacgaggcg ggtgcatttg cccgcatgta tgcacatgat gatgaagagc
      601 caattatcac tgcccatgat attgaacggg taattgcaaa gatagcacgc atacctgaaa
      661 gaacagtttc atccagtgaa attcaaaaat tgcagtattt agaagacgag ttgaaatcac
      721 agatttttgg gcaggatggt gcagtgcaga aggttgtaga ggccataaag cgctcacgcg
      781 ctggattcag ggaagctgaa aaacccattg cttcattttt atttgttgga ccaacaggag
      841 ttggcaaaac tgagcttgca cggcagctat cgttgattat gggtattgaa cttatacgtt
      901 ttgatatgag cgagtatcag gaaaaacata ctgtggcacg gcttatagga gcaccaccgg
      961 ggtatgttgg atatgaagaa ggcggtcagc tgactgaagc cattcgtaag cacccttact
     1021 gtgtattatt acttgatgag attgaaaaag cacaccctga tgtattcaat gcactgttac
     1081 agataatgga ttatgcaacc ttgactgata acacagggcg aaagactgat ttccgcaata
     1141 ccattattat catgacatct aatgcaggcg tgcgcgaaat gaacaagagg gtccttggct
     1201 ttgaaatggc tacctataat atggatgcgg tcaataaaga attagagcgg ctgtttgcgc
     1261 ctgaattcag aaaccgcctt gacgccatag tgtatttcaa tggcatcaat aaagaaatga
     1321 tgcgcaagat agttataaag aaggtaaacg aatttgcaaa acagcttgaa gaaaaaaatg
     1381 tgcagcttgt ctgtacggat gcatgtatcg actggatttc agaaaaagca tattctcccg
     1441 tatttggcgc acgtgaggtt gcccgcatca tacaggaaca tatcaagtca tactttgtgg
     1501 atgctgtgtt atttgggtca cttaaaaatg gtggcagggc aatagctgat gtagtaaatg
     1561 atctggtatc cataactatc gaacaataat atgttgtatt acctggataa ggatgtcatc
     1621 tggttcccgc cggtggagga agccatagaa gatgggttac ttgcatttgg tggagattta
     1681 acaccacagt ggttgcttga ggcctacagc agaggcattt ttccatggtt taatagtgat
     1741 aactctgaga tattatggtg gagccccgat ccgcgtttta ttttgttccc gcaagaatta
     1801 aggctacatc gttcattaaa gcaggtaatg aaaaaaaaga tttttaccgt aagtatggac
     1861 acagcatttc aaaaggttat agctgcatgt gccatgccac ggaaaaaaga atctggtacc
     1921 tggataactg cacgaatgca aaaagcatat ataaagcttc atgctatggg ttttgcacat
     1981 tctgttgagg tgtggcatga cggcatgctt gcagggggcc tgtatggggt gtctttaggg
     2041 aatgcattct ttggtgaatc catgtttacc agtgttgata atgcttcaaa ggttgctctg
     2101 gttactctga atgcagttgc acgaagattg ggttttgcat ttattgactg ccaggtatat
     2161 acagataatc ttaaacgctt tggtgcgcgg tttattgagc gacaagaatt tatgaccctg
     2221 ctcaatgctt ccctgaaaca ttcaacacat cagggcagct ggagttcact gatacacgat
     2281 ggctatgatt atcttgaatg ccttactgga gcaggagctt agattcaagt aaattttctt
     2341 tttttgcttt tatctttgtg taacggattt ttggatactc cataagtgcg gttacaaaaa
     2401 cagaaaacat aaaatacagt gacagtgaaa caacagcaac tgcaatcacc ccaatgataa
     2461 gaataaatat cctggcatat ggtgctgcta caatacggta tgctgaagca ataaccgcac
     2521 ctgcatatcc cagcaaaagc aggaatgctg atgtaaatgt gccatagaat accattttgc
     2581 ccaccttcat tggtatgcct ttaagctgtt cattcatcca tgcagcacct ttttttacat
     2641 actgtgtaaa aagctcagca ataatttcag ccagccgttt tatcatcaat gcagttatga
     2701 ttattccagc aatgattgat gagtatgatg ctattgctat ggcaaaaaca aaaagaacaa
     2761 gcagggcgat agttactgta atatgaatac atgccatgac aagaagagga atgcatatac
     2821 tgataacaat tgacttcagc gaagctttaa tcagtgcgat ggtgctttca aaacgactta
     2881 agtaatcgtc agggtgaagt acaaagtatt cccattcctg tgcgtaccga tcgtcaggtg
     2941 g
//
LOCUS       DAOSZP010000264         2934 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_26436_length_2934_cov_3.148315, whole genome shotgun sequence.
ACCESSION   DAOSZP010000264 DAOSZP010000000
VERSION     DAOSZP010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2934)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2934)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2934
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26436_length_2934_cov_3.148315"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..482
                     /locus_tag="PK348_06540"
     CDS             <1..482
                     /locus_tag="PK348_06540"
                     /inference="COORDINATES: protein motif:HMM:NF013713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65906.1"
                     /translation="CSFDDPRVTIYTEDGAKFIAERKKAYDVILVDSSDPIGPAAVLF
                     SEEFYKNLSECLDDDGIASTQSESIFYHPDVIITLAQYNKKYFKVPLYYYTMVPTYPS
                     GMIGFSFCSKKYHPINDLKEEKAKALVKQLRYYNPDIHKGAFALPQFIRKKLEGILY"
     gene            489..2072
                     /locus_tag="PK348_06545"
     CDS             489..2072
                     /locus_tag="PK348_06545"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Members of the Solute:Sodium Symporter (SSS), TC
                     2.A.21 as described in tcdb.org, catalyze solute:Na+
                     symport. Known solutes for members of the family include
                     sugars, amino acids, nucleosides, inositols, vitamins,
                     urea or anions, depending on the system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/solute symporter"
                     /protein_id="HXK65907.1"
                     /translation="MSAIDWIIVVLYSICVIGIGYVIGKKQKNQADYYIASQKASAWL
                     TGLSLAANQVSAISLIGVPAFIAIKHNGGLTWLQYELALPLSMMFLILFVVPAFRSFQ
                     GFSIYEWLEKRFGKHTRVILALVFLLSRSLATGVAIVATSYVVSVLIGIDLYISIISI
                     SLISLLYTSIGGILADIYTDVMQLVVLLFSTVLFCGIIIYAIDGHTSFTIAYHRIKIF
                     NYASFGIQDGNTFSLAPMLCGGFFLYISYYGFDQSQAQRFLSCENTDAAQKAIIINTI
                     VRFVIVMLYSFLGILLLLLLSVDNTLRSLLTNQQPDMLIPTFIKHYIPAGLKGVVISG
                     ILAASMSSMDSAINSLSAITYNDALKIAYEKVESIHDKTKLLLSRLLTVFWGVITTLF
                     AIIFAGSSETVVELVNKIGSLLYGPICAVFIIGMLTRKKIHQLAVTGGFATGILVNIY
                     IWLFLPSVSWMWWNATGFFAAVLLPFIFSFFTDSEYISDSGYTGKTLPKNTWFKAIVL
                     GIWFMAIIGFFMIIEKVFE"
     gene            complement(2130..2825)
                     /locus_tag="PK348_06550"
     CDS             complement(2130..2825)
                     /locus_tag="PK348_06550"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HXK65908.1"
                     /translation="MYQYPLGTYLTHYTVNKLRNIKPHFLCFPEYFFVNKKLGNHVQT
                     QHNQSLQLKRMQVLSKELNTVVIGGTMPELNGNYLHNTTFVFHNGTMLGFYRKQNLFF
                     AEEGKITPGNSHVIFTAYGIRFSVLICADVFKDEHFIALKELGAQIIFIPTFSPIRNE
                     TIEEKFKRDQDIFVRGAQLANAILVKVCGVKSDYKNFLQARSLIASPHGILYRVEPDQ
                     EDKAMLIVNTVSL"
BASE COUNT          893 a          469 c          551 g         1021 t
ORIGIN      
        1 catgttcatt tgatgatccc cgtgtgacaa tatatactga agatggtgca aagtttattg
       61 cagaaaggaa gaaagcgtac gatgtaattc ttgtagactc atctgatccc attgggcctg
      121 ctgcagtatt gttttcagaa gaattttata aaaatttaag tgaatgcctg gatgatgatg
      181 ggatagcatc gacccagtca gaatcaatat tttatcaccc agatgttatt ataacgcttg
      241 cacagtataa taaaaagtat ttcaaagtcc cattatatta ttataccatg gtgcctacgt
      301 atcccagtgg tatgattggt ttctcgttct gttcaaagaa ataccatcca ataaatgatt
      361 tgaaagaaga aaaagcaaaa gcgcttgtaa agcaactcag atattataat cctgatattc
      421 acaagggagc atttgctttg ccacaattta tacgtaaaaa attagagggg attctgtatt
      481 aatatccaat gagtgccatt gattggatta ttgttgtttt atacagcatt tgtgtcattg
      541 gcatagggta cgtaattggg aaaaaacaaa aaaatcaggc tgattattat atagcttccc
      601 agaaagcatc agcctggtta actgggttat cacttgcagc aaatcaggta agtgctataa
      661 gcctgattgg agtacctgct tttatagcta tcaaacataa tggagggcta acgtggttgc
      721 agtatgaatt agccctcccg ttatcaatga tgttcctcat tctttttgtt gttcctgcct
      781 tcaggtcatt tcagggcttt tcaatctatg aatggttgga aaagaggttt ggcaagcata
      841 cacgtgttat attagcactg gtttttttac tcagcaggtc attggctacg ggcgtagcaa
      901 ttgttgccac gtcatatgtg gtaagtgtat tgattggtat tgatttgtat atttctatta
      961 taagtatttc attaatatca ctactataca cgtctattgg aggaatttta gctgatattt
     1021 atactgatgt tatgcaactt gtagtactac tgttcagtac ggttttattt tgtggaataa
     1081 ttatttatgc tattgatggt catacctctt ttactattgc ttatcatcgg ataaaaatat
     1141 ttaattatgc ttcattcggg atacaggacg gtaatacgtt ttcgttggca ccaatgttat
     1201 gcggcggttt ctttttatat atatcatatt atgggtttga tcagagccag gcgcagcgtt
     1261 ttctttcatg tgaaaacact gatgctgcac aaaaggccat tattataaat actatagttc
     1321 gatttgtcat tgttatgttg tattcttttt taggaatttt actattactg ctattatctg
     1381 tagataatac gttacgctct ttgttaacca atcagcagcc tgacatgttg atacccacat
     1441 ttattaaaca ctatattcct gctgggctaa aaggtgtagt gatttcagga attttagctg
     1501 catcaatgtc aagcatggat tcagcaataa attccttaag cgcaattacc tataatgatg
     1561 cactgaaaat agcctatgaa aaagtagaat ccatacatga taaaacaaaa ctattgttat
     1621 cacgcttatt aactgtattc tggggtgtga ttacaacatt atttgccatt atttttgctg
     1681 gaagcagtga aactgttgtg gaactggtaa ataaaattgg gtcactgtta tatgggccaa
     1741 tctgtgcagt ctttattatt gggatgttaa ccagaaaaaa gattcaccag cttgcagtta
     1801 caggagggtt tgcaacaggc attctggtta atatatatat ttggttgttt ttgccatcag
     1861 tatcatggat gtggtggaat gcaacaggtt tttttgctgc agtactttta ccttttatat
     1921 tctcattctt tactgattca gaatatatca gtgatagcgg ctatactggc aaaactttac
     1981 ccaaaaatac atggtttaag gcgatagttc ttggcatatg gtttatggca ataattggct
     2041 tttttatgat aattgaaaaa gtgtttgaat aattaaagct tatagtaagc atagatttat
     2101 tgtatcatca ataaaagcat catcacacat tataaactca cagtgttcac gataagcatt
     2161 gccttgtctt cctgatcagg ttccaccctg tagagtatgc catggggtga tgcaatgagg
     2221 cttcgtgctt gcaaaaaatt tttataatcg gatttaacgc cgcatacttt aaccagaatt
     2281 gcattggcta attgtgcacc tctaacaaaa atatcctgat cgcgtttaaa tttttcttca
     2341 atagtttcat tccgtatagg tgaaaatgtt ggtataaaga taatttgtgc acctaattct
     2401 tttagtgcta taaaatgttc atccttgaaa acatcagcac agatcaaaac actgaaacgt
     2461 atgccatacg cagtgaaaat gacatggctg tttccaggtg taatcttgcc ttcttcagca
     2521 aaaaaaagat tctgtttgcg gtaaaaacct aacatcgttc cattatggaa aacaaaagta
     2581 gtattgtgca aataattgcc attaagttcg ggcatggtac caccaatgac aacagtattt
     2641 aattcttttg atagtacctg catgcgtttt aactgcaaag actggttatg ctgtgtttgt
     2701 acatgattgc ctagtttttt attcacaaaa aaatattccg ggaaacatag aaagtgaggt
     2761 tttatgttac ggagtttgtt gactgtataa tgagttaaat acgttcccag tgggtactga
     2821 tacatcacta ttgtataatc cataataaag cctttttgat ggaatagaca tttgtactaa
     2881 taattttaac agtaactatc gcataaatag tatatcatta tttttttatt taaa
//
LOCUS       DAOSZP010000265         2931 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_26465_length_2931_cov_3.059458, whole genome shotgun sequence.
ACCESSION   DAOSZP010000265 DAOSZP010000000
VERSION     DAOSZP010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2931)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2931)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2931
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26465_length_2931_cov_3.059458"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(165..611)
                     /locus_tag="PK348_06555"
     CDS             complement(165..611)
                     /locus_tag="PK348_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HXK65909.1"
                     /translation="MESRNVLLIFNQHIMYKPIIYRIAKDFNVIFNVLEAKILPKQEG
                     RLILQLSGEKQDLDACITYLKDEEVVVDILADKIQRDEDRCVHCGACTAVCRTDALVI
                     NRETMMVEFHPDRCVACGLCKLACPVKAMSGISIDVDSEELAVKAV"
     gene            complement(624..1346)
                     /locus_tag="PK348_06560"
     CDS             complement(624..1346)
                     /locus_tag="PK348_06560"
                     /inference="COORDINATES: protein motif:HMM:NF003323.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0280 family protein"
                     /protein_id="HXK65910.1"
                     /translation="MSNTLPYRTKLATRFTTFEVRVETTNLYICAHSNVYDRAIVLVK
                     SLREDIERYSEKHPQFLHALTPLEPEALAPEVVQRMCKASLLAGVGPMAAVAGAIAQM
                     VGEDLLHYSPEVIVENGGDIWAYVREPAKISVFAGRSAFSKHLGIALTPDMTPCGICS
                     SSGRFGRSFSFGKADIATIIADTAALADAVATATCNMVQSDSDCTQAVSFATSISGVK
                     GALVIYWDTMAVKGSIELTEIE"
     gene            complement(1339..2529)
                     /locus_tag="PK348_06565"
     CDS             complement(1339..2529)
                     /locus_tag="PK348_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine biosynthesis protein"
                     /protein_id="HXK65911.1"
                     /translation="MSDIVIKKSYEEINAKIKKGDAVVVTAEEMAGIVKSEGYKSAFK
                     KVDVVTTGTFGTMCSSGAFINFGHTRPKIKASRVWLNDVEAYAGLAAVDIYIGATQVQ
                     EDDPLNKVHPGRFKYGGGHVIEDLIAGKPVKLKAKSYGTDCYPLREYEATVTIHDLRD
                     AFLYNPRNAYQNYNCAVNQSKKTIYTYMGILRPNMGNATYSSAGQLSPLLNDPYYWTI
                     GVGTKIFLGGTVGAVTWRGTQHDPNAPRGDNGVVRSGAGTIAVTGDMKQMSAQFIKGA
                     SITGYGCSLMVGLGIPIPMLNEDLAFFTGVSDDQIFCQVVDYGYDYPNAIGRVIAEVS
                     YAQLKSGFIEIGGKRIPTAPLSSYPMAKKIATILKDWIKASQFTLGQPQILLPSVPYD
                     KRHE"
BASE COUNT          821 a          664 c          614 g          832 t
ORIGIN      
        1 gtccctacac ccgtccctac acccgtccct acacccgtcc ctacacccat ttgcatatcc
       61 gttaacaaca ccgttttcat acacccgata acaacaccat tccacgaacc gtccctacac
      121 ccgtgtgcat atccgttcaa tataacaatc gtataaaacc gtacctacac ggcttttact
      181 gctaattcct ctgaatccac atctatggat atgccgctca ttgccttgac ggggcaggcc
      241 agtttgcata aaccgcatgc tacacaacgg tcgggatgaa attccaccat catggtttca
      301 cggtttataa caagggcatc ggtgcggcat acagcggtgc aggcgccaca gtgcacacag
      361 cgatcttcgt cacgttgaat tttatcggca aggatgtcta caacaacttc ctcatcttta
      421 aggtaggtta tgcaggcatc caggtcctgt ttttcgcctg atagctgcaa tataagcctt
      481 ccttcctgct tgggtaatat tttggcttca agcacgttga agattacatt aaaatctttt
      541 gcaatacgat atatgattgg tttgtacatg atgtgctgat taaaaattaa cagtacattt
      601 cgtgattcca tggtaaacct cgtttattca atttcagtaa gttcaatgct acctttgact
      661 gccattgtat cccagtatat caccagtgcg ccttttactc cagatatact ggttgcaaac
      721 gatacagctt gtgtgcagtc actatcgctc tgtaccatgt tgcatgtggc ggtggctacg
      781 gcatcggcca gtgcggcagt atcagcaatg attgtggcaa tatcggcttt gccaaagcta
      841 aatgagcggc caaacctgcc cgatgatgag cagatgccac agggcgtcat atcaggggtc
      901 agagcaatac ctaaatgttt tgaaaacgct gagcgccccg caaaaacgct aattttagct
      961 ggttcacgca cataggccca tatatcgccg ccgttttcaa caatgacctc agggctatag
     1021 tgcagcaagt cttctcccac catctgggcg atagcgcccg cgactgctgc catgggacca
     1081 actcctgcca gcaacgaagc tttgcacatg cgttgcacaa cctcaggcgc taatgcctca
     1141 ggctcaagcg gggtcagagc atgaagaaac tgtggatgtt tttcactata gcgctcaata
     1201 tcttcccgta agcttttgac aagaactatc gccctatcat atacgttgga atgagcgcat
     1261 atataaagat tggttgtttc aacacgtacc tcaaaggtgg tgaaccgtgt ggcaagcttt
     1321 gttcggtagg gcaatgtgtt actcatggcg tttatcatac ggtaccgatg gcagaagtat
     1381 ttgtggctga cctaaggtaa actggcttgc ttttatccaa tctttaagaa ttgtagcaat
     1441 cttttttgcc atggggtagc ttgaaagtgg tgcggttgga atacgtttgc cgccaatttc
     1501 aataaaccct gattttagct gtgcgtaact tacctcggct atgactcgtc ctattgcgtt
     1561 gggataatcg tagccatagt ccacaacctg gcaaaagatc tggtcatcgg acacgccggt
     1621 aaagaatgcc aggtcctcgt ttaacatggg aataggaatc ccaagtccaa ccataagcga
     1681 gcagccatac ccggtaattg acgcaccttt aataaattgt gctgacatct gtttcatatc
     1741 gccggttaca gcaatggtgc ctgcacctga tcgcaccaca ccattatcgc cgcggggtgc
     1801 attggggtca tgctgagtac cgcgccatgt tacagcacca acagtaccgc ctaaaaatat
     1861 cttggtacct acaccaattg tccagtaata cgggtcattg agcagggggc ttagctggcc
     1921 ggcacttgag taagttgcat tccccatatt ggggcgcagt atccccatgt acgtgtagat
     1981 agtttttttg gactgattaa ctgcacagtt atagttttga taggcattgc ggggattgta
     2041 taaaaatgca tcacgcaggt catgtatggt tactgttgct tcatattcgc gtaagggata
     2101 acaatctgta ccatagcttt ttgctttaag ctttacgggt ttgccggcaa ttaaatcctc
     2161 aatgacatgt ccaccaccat atttaaagcg tcctggatgt actttattga gtgggtcatc
     2221 ttcctgtacc tgtgttgcgc caatgtaaat atccactgcg gcaaggcctg catacgcctc
     2281 aacatcgtta agccacaccc ttgatgcttt aatcttggga cgggtgtgac caaaattaat
     2341 aaacgcaccg gaagaacaca tggtgccaaa ggtaccggtt gttaccacat caactttttt
     2401 aaatgctgat ttatatcctt cagatttaac tatacctgcc atttcttcag cagtgacaac
     2461 aactgcatca ccttttttta tctttgcgtt tatttcttca tagcttttct ttatgacaat
     2521 atcactcatg gtggatttcc tttgtgttat aatgtgtggg ataccattat ggagccagag
     2581 cttgaaaact ggcaagtaaa aatttatgat tgaaaggatg aggagcttgc atgtttaata
     2641 aaaattaaaa aaggcttgaa aaaaagaaaa tgtaataaaa attttatatt taacgacata
     2701 gaaattgcag atatatgaaa atttgtgtct atgtacaaat ggtgaaactt aaaaagatga
     2761 attcaattat gatatattgt aaaagatctt tgtgatggca gaaaaagaag caaaagcaag
     2821 aataaaaata ctgtgtttta taaaaaaatc tatataaatg ttaccaggaa atgaacatga
     2881 acaacgaaaa gattacgcta agacaacttg aaacccatct gtttaaagca g
//
LOCUS       DAOSZP010000266         2910 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_26695_length_2910_cov_3.237128, whole genome shotgun sequence.
ACCESSION   DAOSZP010000266 DAOSZP010000000
VERSION     DAOSZP010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2910
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26695_length_2910_cov_3.237128"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(220..2424)
                     /locus_tag="PK348_06570"
     CDS             complement(220..2424)
                     /locus_tag="PK348_06570"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HXK65912.1"
                     /translation="MNLLQKLNEEQYKAVTHIDGPLLILAGAGSGKTRVITYRIAHLI
                     KNVGVPPFAIVAVTFTNKAAEEMQNRIRALIGPIGESVMIKTFHSLAVYILRRHGEAI
                     GIPSNFTIYDNHDQESVIKDILRDMHIDPKTVKPSMIAEKISRIKESSHFIEGGDITP
                     LLPKFHSFNFPEIFTQYHEKLKTANALDFSDLLVRTVQLLRNKEVLHKLQSRWKYFMI
                     DEYQDTNIAQYLIAKYLSSETKNICVVGDDDQSIYSWRGADIRNILNFEKDYPNATVI
                     TLHRNYRSTEPILKAAWHVIKNNYKRKDKKLTSHRGDGEPIIVCTANNEYGEAEYVIN
                     TIVTLKHKEGYTNRDFAIFYRTNAQSRIFEEHLRKENIPYVVIGSVKFYDRKEIKDII
                     AYMRFIANQHDTVSLLRIINTPARGIGASTIEKIRDAAYVNGVSEWDIISNSETYSYK
                     LSPALTAFKDTMQKLIDKAKEIPQHYKLSDICISILHDTQYKKSLEDENSIESQGRLE
                     NIDAFVNSIFEYEHLHPQATLNEFLQDISLLTPADETNGTLQDAITLMTVHNAKGLEF
                     PVVFLTGMEEGLFPHANSSDTEEGIEEERRLCYVGMTRAKDRLFLTGAEIRRSWGTIN
                     YKEASRFISELPSQLCEFKNYNSLYDSWDTPKSSSFDKFISGSENSSKSIQNEISDSR
                     FRVHQRVKHPSFGIGVITTIQGKGDNVKLTIQFLNGFTKTFLERYTPLEPIS"
     gene            2663..2842
                     /locus_tag="PK348_06575"
     CDS             2663..2842
                     /locus_tag="PK348_06575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65913.1"
                     /translation="MKIYNGIVYCNFDERFSNVISTNVFTLSFQRTFSPCHFNERLYT
                     VISANIFTMSFRRTQ"
BASE COUNT          867 a          517 c          543 g          983 t
ORIGIN      
        1 ataagataaa aaggggaatg tttttctttg agttcacggt aggcattcat aatatcgcta
       61 tctacctctg aggttatatc cccttctaaa ataagaacag gaatattatt cagaatcttt
      121 gataaaaccc gatgcgtttt ttccataatc catgccttta gcaattattt cctgatagac
      181 aaattaacta attttgagta caaattgtca agaaaaaaat tacgatatag gttctaatgg
      241 tgtgtagcgc tcaagaaatg ttttagtgaa cccatttaaa aattgtatgg ttagtttaac
      301 attatcccct tttccctgta ttgtggttat aaccccaatg ccaaatgatg ggtgcttaac
      361 tctttgatga actctaaacc gtgaatcgga tatttcattc tggatgcttt tactgctgtt
      421 ttcagatcca gaaatgaatt tatcaaatga agaagattta ggggtatccc atgaatcata
      481 caatgaatta taattcttga attcgcataa ctgggatggc aactcgctta taaaccgtga
      541 tgcttcctta tagttaatag taccccagct tcttcgtatt tctgcacccg ttaaaaagag
      601 cctgtccttt gccctggtca tgccaacata gcaaagccgc cgttcttcct caatgccttc
      661 ttctgtatcg ctggaattgg catgagggaa taacccttct tccatgccag ttaaaaagac
      721 aacggggaat tccagccctt ttgcattgtg tacagtcata agggtaatgg catcctgcag
      781 tgtgccattt gtttcatcag caggtgttaa taaagaaata tcctgtaaaa attcattaag
      841 ggtagcctgc gggtgcagat gttcgtattc aaaaatgctg tttacaaatg catcaatatt
      901 ttcaagcctt ccttgtgatt caatactatt ttcgtcttca aggcttttct tgtactgtgt
      961 gtcgtgcaaa atactgatgc atatatcaga aagcttataa tgctgtggaa tttcttttgc
     1021 tttgtcaatt aatttctgca tggtatcttt aaatgcagta agggcaggcg atagtttata
     1081 tgaataagtt tcactgttgg aaatgatatc ccattcgcta actccgttaa catatgcagc
     1141 atcacggatt ttttctatag ttgacgcgcc gataccgcgt gcaggagtat tgataatcct
     1201 cagaagtgaa acagtatcat gctggtttgc aataaaacgc atgtacgcaa tgatgtcttt
     1261 tatctcttta cgatcataga atttaacact accaataacc acgtagggaa tattttcttt
     1321 acgcaggtgt tcttcaaaga tgcgcgattg agcattggtg cggtaaaata ttgcaaagtc
     1381 cctgttggta taaccttctt tatgtttaag ggtaacaatg gtattgataa catattctgc
     1441 ttcgccgtat tcattgttgg ctgtacacac aatgattggc tcaccatcac cgcgatgtga
     1501 tgtgagcttt ttgtcttttc gtttataatt atttttaata acgtgccatg ctgctttaag
     1561 gattggttca gttgagcgat agttcctgtg taaggtaatc actgttgcat tgggataatc
     1621 tttttcaaag tttaatatat tgcggatatc agcaccacgc catgaataga ttgactgatc
     1681 atcatcacca acaacacata tatttttagt ctctgatgat agatattttg ctattaagta
     1741 ctgagcaata ttggtatcct gatattcatc aatcataaag tatttccacc gcgactgcag
     1801 cttatgcaat acttctttat tgcgcaaaag ctgtactgta cgcacaagaa gatcgctgaa
     1861 atcaagggca ttagcggttt taagtttttc atggtactgt gtaaaaattt caggaaaatt
     1921 aaatgaatga aattttggta gaagcggtgt aatatcaccg ccttcgatga aatgtgaact
     1981 ttcttttata cgtgaaattt tttcggcaat cattgatggt tttactgttt tgggatcaat
     2041 gtgcatatcg cgcaggatat ctttaataac tgattcctga tcatgattat catagatggt
     2101 aaaatttgaa ggtatgccaa tagcctcacc atgccgacgc agtatataca cagccagcga
     2161 atggaatgtt tttatcatga cgctttcacc aatggggcca atgagagctc ttatgcggtt
     2221 ttgcatctct tcggctgcct tattggtaaa tgtcaccgca acaatggcaa aaggtggaac
     2281 gccaacattc tttataagat gggcaattct ataggtaata acgcgtgttt tacccgagcc
     2341 agcaccagcc agtattaaca gtggcccatc aatatgggtt acagccttat attgttcctc
     2401 gttaagtttt tgtagtaaat tcatgatgta tttttagata atttagaggt agtttattgt
     2461 ttcatttata ttactgcaaa aaaatgcaat aaatttgtga atccccagca cattatttga
     2521 tacctgtact aaaatgacat aattaataat actacgaatt gttcaagata aaaatagtaa
     2581 aaatataatt tttacttgat atatgtgaaa gaaaattatt tttgtggcat agctacttga
     2641 atttatatag gcagaagtat atatgaagat atataatggc atagtttatt gtaattttga
     2701 tgaacgattt tccaatgtta tttcaacgaa cgtttttaca ctgtcatttc aacgaacgtt
     2761 ttcaccatgt catttcaatg aacggttata cactgtcatt tcggcaaaca ttttcaccat
     2821 gtcatttcga cgaacgcagt gaggagaaat cttaatacag cagcatcccg atgaaatcgg
     2881 ggtgaagaat gaccttaagg tttagcacga
//
LOCUS       DAOSZP010000267         2901 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_26790_length_2901_cov_3.177442, whole genome shotgun sequence.
ACCESSION   DAOSZP010000267 DAOSZP010000000
VERSION     DAOSZP010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2901)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2901)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2901
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26790_length_2901_cov_3.177442"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..220)
                     /locus_tag="PK348_06580"
     CDS             complement(<1..220)
                     /locus_tag="PK348_06580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65914.1"
                     /translation="MKKFALIVVFILLASAIYPEGGDYLRVTLVSPKLSYDENDDVIV
                     YCIVHNTAGVPVEFTVYDAPYTTFQPVVY"
     gene            complement(287..2164)
                     /locus_tag="PK348_06585"
     CDS             complement(287..2164)
                     /locus_tag="PK348_06585"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HXK65915.1"
                     /translation="MIPVLLRLKNPINIDDRHHFPRPDLLRLPTYNLNGQWVIVPDRK
                     NRGTMLQWYTKTNVQKILHQWYNIDEFESYPVIVPFPLEADKNNEFHAQSGITPEKVI
                     SHKRYWYFCSFSIEDITTGILHFGACDYKATVWLNGKLLGTHEGGYTPFSFFTHFEDT
                     NYLAVLVEDSLSPSQVRGKQTFLNKPFMVWYTNITGIWQDVWVEKVGSSYIRSVTCQR
                     TGSSVRFIIDVVTSENIANDFRVELFIYASQVHGKKSALKTPVKTYRDIVMFDVFKKG
                     QVIFEVPESLFATWSPQYPALHPLQIVLKKGTKEADCVYSYFGRRDIEIKNGSILLNK
                     KNLYQRLLLNQGYYPEGLYRPGDSSMYRTDIELMKSMGFNGCRIHQKIEDPKFLYWAD
                     LLGFLVWEEMPSYYLPLPKNMKKLYTQLQEVMKRDCMHPSIITLVLYNESWGLYSMFF
                     SKKVRDSLMALYTRVKSDFPGYMVIDNSGFHHVKSDIADIHHYLKRFEDIEEFYRRLV
                     AGVREAPLWANFISMILGKENIQTPYLKGYADSQAPLLISEMGGFGFGMYQSEAKTLE
                     ESFQKHCAILAKFPHIQGFCYTQFTDTFQEKNGVCTFDRTMKAKLLPIMQKALSKHIF
                     W"
     gene            complement(2169..>2901)
                     /locus_tag="PK348_06590"
     CDS             complement(2169..>2901)
                     /locus_tag="PK348_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65916.1"
                     /translation="GVRVEHPRELIDEIQYGKGKDGYGLHGAEYQLTFKDVTGRGVYS
                     FCMCPGGFVINSSSQEERLCVNGMSYSKRDGTSSNAALVVTVSQDDFKDIFDGINFQI
                     LLEEKAYQAGGGGFGAPAQRITSFLQNSIDSSLPVCTYKPKIAASNFNTILPDFIRDP
                     LHRAIVHFGSKMKGFITNEGILVGVETRTSSPIRIVRDENFQSINCKGLFPVGEGAGY
                     AGGIVSSAVDGIRAAQKIVTLLERV"
BASE COUNT          885 a          639 c          491 g          886 t
ORIGIN      
        1 catataccac aggttgaaat gtggtatagg gggcatcata cacggtaaat tcaaccggta
       61 caccagcagt gttatgcaca atacagtata caatgacatc gtcattttca tcataggata
      121 attttgggct taccagtgtt accctaaggt agtctccacc ttcgggataa atagcacttg
      181 cgagcaggat gaaaacaact atcaatgcaa attttttcat atcattatct atcgtacaat
      241 gaatattata ccttactcat tttatcgact gtaatgtata tatttgttac caaaatatat
      301 gttttgacag cgctttttgc atgatgggca acagttttgc tttcattgta cggtcaaacg
      361 tgcatacgcc atttttttcc tggaatgtat cggtaaactg ggtataacaa aaaccctgaa
      421 tgtgaggaaa ttttgcaagg atggcacagt gtttctgaaa actttcttcc agggttttag
      481 cctccgattg atacatgcca aaaccaaatc cacccatttc acttattaaa agcggtgcct
      541 gcgaatcagc atatcctttt aaatacggtg tttgaatatt ttctttgccc aaaatcatgg
      601 atataaaatt tgcccacagt ggtgcctcgc gaacacctgc aacaagcctt cgataaaatt
      661 cctcaatatc ttcaaatcgc ttcagataat ggtgtatatc ggcaatatcg ctttttacat
      721 gatgaaatcc gctgttatct atgaccatat atcccggaaa atcagatttt accctggtat
      781 acaatgccat gagactatcg cgcacctttt tagaaaaaaa catggaataa agcccccagc
      841 tttcattgta tagcacaagc gtaatgattg acgggtgcat acaatcccgt ttcatgacct
      901 cctgaagctg tgtatagagc tttttcatat ttttgggtaa tgggagatag taactgggca
      961 tttcttccca cactaaaaat cctaaaagat ctgcccagta taaaaatttt ggatcctcta
     1021 tcttctgatg aatacggcat ccattaaaac ccatactttt catgagttct atatcagttc
     1081 tgtacatgga gctatcacca ggtctataca gcccttcagg ataatacccc tgattgagca
     1141 acaaccgctg atagagattt tttttattta aaagaataga gccatttttg atttcaatat
     1201 cccttctgcc aaaataacta tacacacagt cagcttcttt tgtccctttt ttcaggacta
     1261 tctgaagagg atgaagtgca ggatactgtg ggctccatgt agcaaaaagc gattcaggta
     1321 cctcaaatat tacctgtcct tttttaaaaa catcaaacat cacaatatca cgatatgttt
     1381 tgacaggtgt ttttaacgca ctttttttac catgtacctg tgatgcatag atgaacaatt
     1441 caaccctgaa atcatttgca atattttcgc tggttaccac atcaataata aagcggacgc
     1501 tactgcccgt tctctggcag gtaactgaac gtatatacga tgacccaacc ttttcaaccc
     1561 agacatcctg ccatatgccg gttatattgg tataccatac cataaagggt ttattaagga
     1621 atgtctgttt accccgcacc tgcgatggtg aaaggctgtc ctcaaccagt accgctaaat
     1681 aatttgtatc ttcaaaatga gtaaaaaaag aaaaaggagt gtatccgccc tcatgtgtgc
     1741 ctaaaagctt accattgagc cacactgtag ctttgtaatc acaggcacca aaatgaagta
     1801 ttccagtagt aatatcttca atggaaaatg agcagaaata ccagtaacgc ttatgcgata
     1861 tcaccttttc tggtgttatt ccgctttgtg catgaaactc gttattttta tcagcttcca
     1921 gcgggaatgg gactataaca ggatatgatt caaattcatc aatgttatac cattgatgca
     1981 gtatcttctg aacattggtc ttagtatacc attgcaacat tgttccacga ttttttctat
     2041 caggcactat cacccattgg ccattgaggt tgtaggttgg caatcgtaat aaatctggcc
     2101 gtggaaaatg gtgtcggtca tcaatattga ttggattttt taatcgcaat aaaaccggaa
     2161 tcattgtttt ataccctctc taataatgtt actatctttt gtgctgcacg aataccatca
     2221 actgccgaac taacaatccc gcctgcatac ccggcacctt cgccaacagg gaataaccct
     2281 ttacaattaa ttgactgaaa attttcatca cgaactattc gtatgggaga agatgtcctg
     2341 gtttctacac ccactaaaat cccttcgttt gtaataaaac ctttcatttt tgatccaaag
     2401 tgcacgatag ccctgtgtaa tggatccctg atgaaatcag gaagaattgt attaaaatta
     2461 cttgcagcaa tctttggttt atacgtacat accggcaatg aagaatcaat cgaattttgc
     2521 aaaaaagatg taatacgctg ggcaggagca ccaaatccac ctccacctgc ctgatacgct
     2581 ttttcttcta acaaaatctg aaaattaatg ccatcaaaaa tatctttaaa atcatcctgt
     2641 gaaacggtaa ccaccagagc agcatttgaa ctggttccat cacgctttga gtaactcatc
     2701 ccgttcacac acaatcgttc ttcctgggaa gaagaattaa taacaaatcc acccgggcac
     2761 atacaaaatg aatacactcc tctccctgtg acatctttaa aggttaattg atactcagca
     2821 ccatgcagac catacccatc ttttccctta ccatactgaa tttcatctat cagttctcgt
     2881 ggatgttcaa cccgtacacc c
//
LOCUS       DAOSZP010000268         2899 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_26812_length_2899_cov_3.543601, whole genome shotgun sequence.
ACCESSION   DAOSZP010000268 DAOSZP010000000
VERSION     DAOSZP010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2899
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26812_length_2899_cov_3.543601"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..507)
                     /locus_tag="PK348_06595"
     CDS             complement(<1..507)
                     /locus_tag="PK348_06595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HXK65917.1"
                     /translation="MRKFLLVQVAIVVVAAFAISCAPNNVMTKRDETYTFDQMSVQDE
                     TPVYSDMEPTQISSNSSDSSYYQVGIASWYGREFQGRLTASGEPFDMNKYTAAHRTLP
                     FGSVILITNLENGKKVKCTVNDRGPFKKNRVLDVSYKAAKDLDMLTTGEAKVGITVLS
                     YGNNTQPGT"
     gene            669..1703
                     /locus_tag="PK348_06600"
     CDS             669..1703
                     /locus_tag="PK348_06600"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HXK65918.1"
                     /translation="MKIVSSKPTITRKELEGVLDCLIQDTLASGAVVKQFEQALGNLL
                     GMKYALATNSVTAAYHCAFMALGVTGGDEIIMPSFFHEAPLKACHLVGATPVLIDIAE
                     GAYHPSPELIQKAITQKTKAIVLGHMFGIPLGFDLGDSSVPVVLDISHAIGMDTADIF
                     LPSPSIAIASFSPSMMITTEMGAMVFTNNSRHYSYIRDVRNGVPHSHIPGFEYCMSDL
                     HAALGLNQLLKLKDFVRRRRDIARIYYEALNKTHHTTVYAYNDHCTYQAFPVLFDAQP
                     DIAEKFFKKNGIEIFHPVEVPLHQYCKLRNLDYPHSDRLSKKLFSLPIYPTLTKREIE
                     KVSRVLTMFA"
     gene            1726..2121
                     /gene="queD"
                     /locus_tag="PK348_06605"
     CDS             1726..2121
                     /gene="queD"
                     /locus_tag="PK348_06605"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: protein motif:HMM:NF013411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HXK65919.1"
                     /translation="MYILTIEDYISAAHQLKGYKGKCENIHGHNWKVEVSVYGHKLND
                     IGILIDFHDLKDMLKTVLQEFDHKNLNDIQEFAHQNPSSELLSKIIYQKIEKMLKQYS
                     QNHSTNINVLSVTVWESATCRSTYQKKFF"
     gene            2212..2358
                     /locus_tag="PK348_06610"
     CDS             2212..2358
                     /locus_tag="PK348_06610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65920.1"
                     /translation="MKKFASLILIAVFAVAVAFVGCKKEEAPAPATDQPVQEQPAEEA
                     PAQK"
BASE COUNT          913 a          539 c          557 g          890 t
ORIGIN      
        1 tgttcctggc tgtgtattgt tgccgtatga aagaactgta atgcccactt tagcttcgcc
       61 agtagtaagc atatccagat cctttgcagc cttatatgat acatcaagaa cgcggttttt
      121 cttgaatggt cctctgtcat taacagtaca ttttaccttt ttaccatttt caagattggt
      181 gataagtata acactgccaa atggaagtgt gcggtgtgct gcagtatatt tattcatatc
      241 aaatggttct ccagatgcgg tgagccttcc ctggaactct cgtccatacc agctggctat
      301 gcctacctga taatatgagc tatcgctact atttgatgat atttgagttg gttccatatc
      361 agaatataca ggggtctcat cctgtacact catctggtca aatgtatatg tttcgtcacg
      421 tttggtcatg acattattag gtgcacatga aatagcaaaa gctgctacaa caactatcgc
      481 cacctgtact aataaaaatt tacgcattga atgcctcctt tgataatacc tatactatgt
      541 gtagtatcgg aataacgtaa ccgttgcttg atttaatttt tattcttgcc aaatgatgct
      601 tgaccaattt ttaataatac gcacatgtac gtttcatttg aatattatat agtaggcaat
      661 agagaaccat gaaaatagtt tcaagcaaac caactattac acgaaaggaa ctggaaggtg
      721 tacttgattg tcttattcag gatactctgg catccggtgc tgttgtgaag caatttgaac
      781 aggcactggg aaatctattg ggaatgaagt atgctctggc aaccaattca gttactgcgg
      841 catatcactg tgcctttatg gcgctgggtg ttacaggggg tgatgagatc ataatgccat
      901 ctttttttca tgaagcgcca ttaaaggcat gccatctggt tggagcaaca ccggtattaa
      961 ttgatattgc tgaaggtgct tatcatcctt caccggaatt aatccagaag gctattacac
     1021 aaaaaactaa agcaattgtg ttgggccaca tgtttggcat accacttggt tttgatttgg
     1081 gcgatagttc cgttccagta gtccttgata tctcacatgc cataggtatg gacactgctg
     1141 acatcttcct gccatcacca tcgattgcaa ttgcatcatt ttcgccatcg atgatgatta
     1201 caacagaaat gggcgcaatg gtatttacca ataattcgcg ccattattca tacatacgtg
     1261 atgttcgaaa tggtgtgccg cattctcata ttcccgggtt tgaatattgc atgagcgacc
     1321 ttcatgctgc tcttggctta aaccagttgc ttaaactgaa agattttgtc aggcgaagac
     1381 gtgatattgc ccgcatatac tatgaagcgt tgaacaaaac acatcacacc acagtatatg
     1441 catataacga tcactgcaca tatcaggctt ttccggtact ctttgatgca caacctgata
     1501 tagcagaaaa attttttaag aagaatggca ttgagatatt ccatcctgtt gaagttcctt
     1561 tgcatcagta ctgtaaactc aggaatctgg attatccaca cagtgacagg ttgtcaaaaa
     1621 aacttttttc gttgcccatc taccccacac ttaccaaacg tgaaatagaa aaagtaagca
     1681 gggtgttgac catgtttgca taaacgatta atcaggagca caggaatgta cattttaaca
     1741 atagaagatt atatttcagc agcccatcag ttaaaagggt acaaggggaa atgtgagaat
     1801 attcatggcc acaactggaa agtagaagta tcggtgtatg ggcacaagct taatgatata
     1861 ggaatattaa ttgatttcca tgatctgaaa gatatgctta aaacagtatt gcaggagttt
     1921 gatcacaaaa atttaaatga tatacaggag tttgctcacc agaaccccag ttcagaattg
     1981 ttatctaaaa ttatatatca aaaaattgaa aaaatgttaa aacaatattc tcaaaatcat
     2041 tcaacaaaca ttaatgtact cagcgtgacg gtatgggaat ccgcaacatg ccgttcaact
     2101 taccagaaaa aattttttta atatatactt gattttttta tatggctgtg tttattggtg
     2161 tagccatact caaacacaat aaatctttaa tattataaaa ggagtaacgc catgaagaaa
     2221 ttcgcatctc ttattcttat tgctgttttc gcagttgctg tagcatttgt tggctgcaag
     2281 aaagaagaag ctccagctcc tgctactgat cagccagttc aggaacagcc agcagaagag
     2341 gctccagctc agaagtaatt acaatgttct tgcttgcaaa aagaggggca ttgcaatgcc
     2401 cctctttttg ttttcatacg tgatttccat aactttataa tcaaagagca catgattcct
     2461 aatcgtaagg gtatcaggta ctctatggag ggtgagcaac tatgatgtga tgcgccatga
     2521 gatgatcaaa gagaggaaaa attgaaaaat atttaagata tgatttatca tcactatatc
     2581 agcaagaatg aaatgaaata atggaatcag ctgtatagag aacaattact taaaaataga
     2641 atatttgccc atgacgactt gcatgtgata ggagtataga cgtttttata ttagttcact
     2701 gtttacattc aggaaaaaat aatgcaatca atatactatt attcatcccg tttatagcgc
     2761 ttgatattgg gatgacgtat tgaatatgtg ataactacat aatctggatt gattgaaaag
     2821 ccatctacaa taccatcttt tacaacatca acttcttcag aaaaaatacc acgggcaata
     2881 tactgggcat tgagtagtg
//
LOCUS       DAOSZP010000269         2886 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_26985_length_2886_cov_2.843871, whole genome shotgun sequence.
ACCESSION   DAOSZP010000269 DAOSZP010000000
VERSION     DAOSZP010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2886
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26985_length_2886_cov_2.843871"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            69..1889
                     /locus_tag="PK348_06615"
     CDS             69..1889
                     /locus_tag="PK348_06615"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HXK65921.1"
                     /translation="MKISNIYLAVYTFILFMILSVNYGYADTNITIHPDKKVLNVKPN
                     AKACAIIHIELPSKGYIYANPKGEGTGKATTAYAKPTQHIVPEKTRYLPGTLYISPLD
                     SKPVYIYNKHTALCIPFEVKTTHEGTYSLSITIEALYCNDVSCTPIQQTITQIINVNT
                     SAPEFDTSLCTMYKVPEAIIHTVNKKDLPGHFNVIDAGSTPTSSLFHAIMFGILAGFL
                     LNFMPCVLPVISLKILSFISYGNNKPGQVRIMGLLFTAGILTSFLALASLAAFAGYNW
                     GELFQHKMFIIIMAAIIFALALSFFGVYTFNIPRLPQLQSANIYVDSYFKGIVATLLA
                     TPCSGPFLGATLAWSLTQHPFIIFMVFINIGLGMSLPYLLLSFYPSLIRFIPKPGDWM
                     ITFEQFMGFLMIATTMYLITLLDASTLTKTIWFLLLVSIGLWQWGRFGNITKPSIQRI
                     ISFVVLVLLVVSASMIFSSKKTSQAEITYKPYDWEAIVHNNTSQVAMVIFTAQWCPNC
                     KFVEATALSSSDVIEFIHKNNIAVYKADITTKNPPAETILQQLGSRSIPFVAIFPSGD
                     NFSQPVILRDIYTAKDLLKALQKAIELSKQSEFPQLLTIP"
     gene            complement(2362..>2886)
                     /locus_tag="PK348_06620"
     CDS             complement(2362..>2886)
                     /locus_tag="PK348_06620"
                     /inference="COORDINATES: protein motif:HMM:NF014856.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide synthetase C
                     domain-containing protein"
                     /protein_id="HXK65922.1"
                     /translation="ILQPVILGMKNEGMSFVGILYAGLIIKGHDIKVLEFNVRFGDPE
                     TQVLLPLINGKLGDFIQAAISGKLSSMNLSFRNKHAITVVLASGGYPGSYKTGLPITG
                     LEQLPEDILVFHAGTRRDGQLLPSGGRVLNITAIADSLEDAYDKVYNAIPSVSFEDMH
                     YRKDIGFRALKKRP"
BASE COUNT          910 a          598 c          479 g          899 t
ORIGIN      
        1 acagcctatc aaagcaggga ataaagttga aataaataca ttttattttc agaaggagac
       61 tcttcataat gaaaattagc aatatatatt tagcagtgta tacgtttatt ttgtttatga
      121 ttttatctgt aaattatgga tacgctgata ctaacattac gatccatcct gataagaaag
      181 tactgaatgt aaaaccaaat gcaaaagcct gtgctatcat acacatagag ctaccttcaa
      241 aaggctatat ctatgcaaac cccaaaggtg agggaactgg taaagctaca accgcgtatg
      301 caaaaccaac gcaacacata gtacccgaaa aaacgcgtta tcttccaggg acattgtata
      361 tttcacctct tgacagcaaa ccggtatata tttacaataa gcacactgca ctctgtatcc
      421 catttgaagt aaaaactacg catgagggta cgtattcatt atcaatcact attgaggcat
      481 tatattgcaa tgatgtatcc tgtaccccta tacagcaaac tatcacccag ataattaacg
      541 tcaatacatc agcacctgaa tttgatactt cactatgtac tatgtataaa gtgcctgaag
      601 ctataattca tacagtaaac aaaaaggatt tacccggtca ctttaatgta atagatgcag
      661 gcagtactcc aacatcaagt ttatttcacg ctatcatgtt tggcattctt gcaggctttt
      721 tgcttaactt tatgccctgt gtgcttcctg taattagtct taaaatttta agttttatat
      781 cctatggcaa taacaaacca ggccaggtac gaatcatggg attgcttttt accgcaggga
      841 ttctcacatc atttctggca ttagcatccc tggcagcatt tgctggttat aactggggag
      901 agctttttca gcataaaatg tttattatta tcatggcagc aataattttt gcactggcat
      961 tatcgttttt tggtgtgtat acatttaata ttccacgact tccacaattg caaagtgcca
     1021 atatatacgt agattcctat ttcaaaggca tagttgccac actacttgct accccctgta
     1081 gcggtccatt tttaggggct acactggcat ggtcattaac gcaacatcct tttataattt
     1141 ttatggtttt tattaacata ggccttggca tgtcactgcc atacctgctt ttatcattct
     1201 atccatcact tatcaggttc atccccaaac ctggtgattg gatgataaca tttgagcaat
     1261 tcatgggatt tttgatgatt gccaccacca tgtatcttat aacattactt gatgcaagca
     1321 ctctaacaaa aactatttgg tttttattac ttgtatccat tgggttatgg caatggggac
     1381 ggtttgggaa tataacaaaa ccatctatac aacgaataat ttcatttgtt gtacttgtgc
     1441 tacttgttgt ttcagcaagc atgatatttt cttccaaaaa aacgtctcaa gctgaaataa
     1501 cctacaagcc ttatgactgg gaggcgatag ttcataacaa tacatcacag gttgcaatgg
     1561 tcatttttac tgcacagtgg tgcccaaact gcaaatttgt tgaagcaaca gcattatcat
     1621 cttcagatgt tatcgaattt attcataaga ataatattgc tgtatacaaa gccgatatca
     1681 ccacaaagaa tcccccagca gagaccatac tgcagcaact gggttcacga tcgataccct
     1741 ttgtagctat attcccttca ggggataatt tttcacaacc tgttatcctg cgcgatattt
     1801 atacagcaaa ggatttattg aaggctttgc aaaaagccat tgaattaagc aaacaatcag
     1861 agtttcctca acttttaact atcccttaaa ggcatgataa tgcaatcact cagatactac
     1921 cgtgaactta tacaaaaaat tgatgcattt actgagtcta ttaattcaac gtatggcaac
     1981 caattacatt gcagggaggg ctgcagcagt tgttgtatac tggaaagtat aatgcctgtt
     2041 gaacttgtgc ccatagtaca atggtatgta acacaacctg aatcaataaa aactataatt
     2101 aaacaagaaa actctcattc ctgcatattt ttgcaaaaca atagctgtat catctatcct
     2161 gtaagacctg taatttgcag aacccatgga tacccggttt taatacgtaa cagtgttgat
     2221 atatgcccat taaataatgg gataaacttt gagccacagt atattctcaa tttagagaac
     2281 tgtaacacaa tgcttgcagc tattaatatt ttattcatta aggaagtaca aattcctgca
     2341 ttacagacag atcgcattaa cctacggacg ttttttcaaa gccctgaatc cgatatcctt
     2401 tctatagtgc atatcctcaa aggacactga tggtatagca ttatatactt tatcgtaggc
     2461 gtcttccaaa ctgtcagcta tcgctgtaat attaagcact cttcctccac tgggaagtaa
     2521 ctgtccatcc cgtctggtac ctgcatgaaa aacaaggata tcctcaggca actgttccaa
     2581 tcctgtaatt ggcaaacctg ttttatatga gccggggtag ccacctgatg caagtaccac
     2641 tgtaattgca tgcttatttc taaatgaaag attcatactg gaaagtttcc cactgatagc
     2701 tgcctgtata aaatcaccca atttgccgtt tatcagcggt aacaggacct gtgtttcggg
     2761 atcaccaaag cgtacattaa attccagtac tttaatatca tgtcctttaa ttattaaacc
     2821 tgcatacagt atcccaacaa aagacatgcc ctcatttttc atgcccagaa tcaccggttg
     2881 aagaat
//
LOCUS       DAOSZP010000270         2885 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_26994_length_2885_cov_3.555477, whole genome shotgun sequence.
ACCESSION   DAOSZP010000270 DAOSZP010000000
VERSION     DAOSZP010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2885)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2885)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2885
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26994_length_2885_cov_3.555477"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            412..1176
                     /locus_tag="PK348_06625"
     CDS             412..1176
                     /locus_tag="PK348_06625"
                     /inference="COORDINATES: protein motif:HMM:NF027439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HXK65923.1"
                     /translation="MLQKKVEDNVCILTLNDGKMNALTDTVLNDLRNYLKEAAADTSI
                     KGVILTGEGKTFSSGFSLPMFLSFKDVDEVVKFFEVEEEILLELFMFEKPVIAALNGH
                     TVAGGMIFAMACDYRIMKNHPKIKMGMSEIKIGLPLSIAQWNVVRFGLDSDKKFRDIM
                     YFGNMFGPEQALQLGLIDELVDEDKLISRAKELINLWKNNPGNAFTPLKLCIRKETAE
                     RIRKELKEGDMKKELKCFFDENVRKTLEFVQAAMSK"
     gene            1237..>2885
                     /locus_tag="PK348_06630"
     CDS             1237..>2885
                     /locus_tag="PK348_06630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65924.1"
                     /translation="MNIKEAGWKIFSLRSEEVSFTIPLFFLYLFSGIFFATGQVYTES
                     LFLQTYGAAGLSKFFAINGIVLFLSGFIYTYFLELFSLKRAYILLIVLFCCIPGSTLL
                     PSGWAPHDPLYLFIGNYIATFYLDAHFINFSFQFLTLQSSKRIFPFLMAGSKLGGIII
                     TIIISLYSTLFIHYAALLWLAAGILILIPFFATAKINASTRSHSEHKFLDKLSTLFSS
                     RMFIVSAIAVFVMAIANQHTEYFFARIATHLFPSQETLAVFISRYTFITDFITVAMQL
                     FVTSRIIKKLSIPTANLLYPSSYCLIIIYAVLSPTLLAAVALRFFRKNMSFFIRQPVS
                     NIMLSIYPADRVNQARSIINSIVSPLGMIAGGLLLSLYATLPLHTVFIIALAIGLLFV
                     TITIKQNALYKEAIYFKLQPQLPNTLQISGSDIIEIVKNPNIQNKSEFIDTLLQQGMS
                     AELFPMIIQYYDVLSENTKIHLLSIIYEAPEERAVEICKKAVNDENPLVRVYAYRFLS
                     QCAIVTRKEILLQSNPLLAIEPLIQSLLINDNAYSEESIINT"
BASE COUNT          922 a          566 c          496 g          901 t
ORIGIN      
        1 aaccttaccc ccagcccctc tccttcgcaa ggatatgggt taaaggtaag atgtgtaaat
       61 attttcattt tgtttaaaaa tccacagtaa tagacaaaaa cattacttac aaaccatgca
      121 gcaaaataaa acatcccaga atcaatgagt ttatacctta tgtggtaata gtcaacccaa
      181 taaaacaatg gttaaaaaat acaaaataca ttattttaga agattatttt atcaataaaa
      241 gcaaccattt ctctcaaata ataccataag gagcaacaaa attaacacat gtaggctgca
      301 taaaaaggtc aagagtattt ttccaaaaat ttcttcttta caacttttac gcctgtacaa
      361 tattgtgtac atatatagtt aaatttttat tacacataat gggggcatgg tatgttgcaa
      421 aaaaaagtag aagataatgt ttgcattctg accttgaatg atggcaaaat gaatgcactc
      481 accgacacgg tgctgaatga ccttcgcaat tatcttaaag aagcagcagc tgatacttca
      541 attaaaggtg ttattttaac cggggaaggc aaaacgtttt catccggatt cagcctgcct
      601 atgtttttga gttttaagga tgtggatgaa gtagttaaat tttttgaagt tgaagaagaa
      661 attttactgg aactgtttat gtttgaaaag ccagtcattg cagcattaaa tggtcatacc
      721 gttgccggcg gtatgatttt tgcaatggcc tgtgattacc gaatcatgaa aaaccatcca
      781 aaaatcaaga tgggtatgag cgaaattaaa attggtctac ctctttctat tgcgcaatgg
      841 aatgtggttc gatttggcct tgatagtgac aaaaagttca gagacatcat gtattttggc
      901 aatatgtttg gccccgaaca ggcgttgcaa ttaggcctca ttgatgaact tgttgatgag
      961 gataagctga taagcagggc aaaagaactt ataaatctct ggaagaataa tccgggcaat
     1021 gcctttactc ctctgaaact atgtatacga aaagagactg cggaaaggat tcgaaaagag
     1081 ctaaaagaag gcgatatgaa aaaagaattg aagtgtttct ttgatgaaaa tgtccgcaaa
     1141 accttagaat ttgtacaggc agcaatgagc aagtaatttt tatgtccgat agttccgata
     1201 atacaagcaa aggagctatg ggacttcatc tttattatga atataaaaga agcgggatgg
     1261 aaaatatttt cactgcgcag tgaggaagta tcattcacca tcccgctttt ctttctgtat
     1321 ctgttctcag gaatattctt cgcaactggt caggtatata ccgaatcact gtttttacaa
     1381 acatacgggg cagccgggct atccaaattt tttgcaatta atggcattgt attattcctt
     1441 tctggattta tctacaccta tttccttgaa ttattttctt taaagcgggc atatattctg
     1501 ctcattgtgt tattctgctg tatacctggc agcacactac ttccttcagg ctgggcacca
     1561 catgaccctc tttatctttt tattggcaat tatattgcaa cattttattt agatgctcat
     1621 ttcattaatt tttcatttca gttccttacc ctgcaaagtt ccaaacggat attcccgttt
     1681 ttaatggctg gatccaaatt aggcggaata attattacca ttataatttc cctgtacagt
     1741 acactcttta ttcactatgc agcgttacta tggcttgcag ccggtattct gatacttatc
     1801 ccattttttg caactgcaaa aatcaatgcg agcacacgta gccattcgga acacaaattt
     1861 ttggacaaac tatctactct gttttcaagc cgcatgttta ttgtaagcgc tattgcagtt
     1921 tttgttatgg caattgccaa tcaacacacg gagtattttt tcgcacgcat cgccacacat
     1981 ctttttcctt cacaggaaac actggctgtc tttataagca ggtatacatt tataaccgat
     2041 ttcataacag tagccatgca actttttgtt acatcacgca tcattaaaaa attaagtatt
     2101 ccaactgcca acctgctcta tccctcatca tactgcttaa ttatcatata tgcagtgtta
     2161 tcacccacat tgcttgctgc tgtagcattg cgttttttcc gcaaaaatat gagcttcttt
     2221 attcgtcagc ctgtcagcaa tataatgttg tctatctatc ctgctgacag agtaaaccag
     2281 gcgcggtcca tcatcaacag cattgtaagc ccactgggca tgatagcagg cgggctatta
     2341 ctttctctat atgcaacact tccattacat acggttttca tcatagctct tgcaattgga
     2401 ttattatttg tgaccataac cataaaacag aatgccctgt ataaagaagc aatctacttc
     2461 aaattacaac cacagctacc aaacacacta caaatatctg gcagtgatat cattgagatt
     2521 gtcaaaaacc caaacataca aaataaatct gaatttattg atacgctgct acagcagggt
     2581 atgtcagcag aactgtttcc tatgataatc cagtattatg atgtgctatc cgaaaacacc
     2641 aaaatacacc tgctttcaat tatatatgaa gcaccagaag aacgtgctgt tgaaatatgt
     2701 aaaaaagcag taaatgatga aaacccgctg gtacgagtat atgcctatcg ttttctatca
     2761 caatgtgcga tagttacccg taaggaaatt ttgctacaat ctaacccact acttgcaatt
     2821 gaacctttga ttcaatcttt gctgataaat gacaatgcat acagtgaaga atccatcatc
     2881 aacac
//
LOCUS       DAOSZP010000271         2883 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_27013_length_2883_cov_3.539604, whole genome shotgun sequence.
ACCESSION   DAOSZP010000271 DAOSZP010000000
VERSION     DAOSZP010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2883
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27013_length_2883_cov_3.539604"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..459
                     /locus_tag="PK348_06635"
     CDS             <1..459
                     /locus_tag="PK348_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003491258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HXK65925.1"
                     /translation="DSIKRNRGRRGLDILNKMQKDQRILVKISDVDFPEVNEVDAKLV
                     KLAKLMNAKIITNDFNLNKVAEFHGVKVLNINQLSNALKPIVLPGEEMAVSLIKEGKD
                     PNQAIGYLDDGTMVVVENGRSRLNSNVDVIVTSVLQTTAGRMIFARIKED"
     gene            437..1021
                     /locus_tag="PK348_06640"
     CDS             437..1021
                     /locus_tag="PK348_06640"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HXK65926.1"
                     /translation="MPGSKKTDATIVALCGSPHIQGNTATALRLLFNELSGISKRIYF
                     AYKLAVQPCTGCGYCSRKFGCIFDDAMNELYDTLQRAHVIIIASPLYFSHLPSPLKSI
                     IDRCQVLWEYYNRGNKPLHLQYGFALLLGAGNYSNMFVPSVITLRHYFNSLNYRFDEK
                     KYLLLSDINNKKDIRNDDITKKITAAANYIQKLL"
     gene            1034..2278
                     /locus_tag="PK348_06645"
     CDS             1034..2278
                     /locus_tag="PK348_06645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65927.1"
                     /translation="MKLYVTLTFSCTSEDLALFVKKVVESGCSIRSINYISSEGNYAT
                     YKADLMYTDRKIFKEKILSWVSKETTLIDCSDIMSKGIAGGLLKVSSKIPFDSAGEFE
                     GKILGYIEILKDNLITEESRKLTGMSSNCGMIILTEDSQQIQPKEIYHYILIERDSLV
                     LSHCSTLNPQPLLMRYRTKEDILKIVDSVSTNYALMRIAMPDTFQEVSLFHQMDEIQS
                     LVLSREYDEIPLYCLIKIMNILKRHHVSLADSVIGIAGITTSSLRLTRLLYGIGCSKV
                     LGFDTSEVNLYHFEKEKGIATNIDNVFENADVVLFMSDCCTSEDFYKLGKGQIVLINP
                     AIHFDTLVFQERGLKDYELLITENSYLLLPGLAEKMIQGQIHSLNDEQLIAIGKSLYG
                     ANLDIDSAENMYSITETIRKVS"
     gene            complement(2284..>2883)
                     /locus_tag="PK348_06650"
     CDS             complement(2284..>2883)
                     /locus_tag="PK348_06650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65928.1"
                     /translation="SNAITIIQKHELIANLTHLLNMDNGGRKYYLLEYEHISEMISNV
                     TEQFYDDLIIINSSYTILYSMKNIDLYGRRLSQLSPVLYYFVSNLPYDDIAIFKPFEY
                     PPMSGNYSFCVCYPIQLDDRNKGFCIGLINQNKFYDILPADSIIAETNGEVIFNIIQR
                     GTLKLDEDTLKKSSINISGKKYNISLLKYKNITWYILYP"
BASE COUNT          948 a          457 c          567 g          911 t
ORIGIN      
        1 gattctatca agcgtaacag aggaaggaga ggcctggata ttttaaataa gatgcaaaag
       61 gaccagcgta tccttgtgaa gatatccgat gtggatttcc cggaagtcaa tgaagttgat
      121 gccaaattgg tgaaattagc caagctaatg aatgcaaaaa tcattaccaa tgattttaat
      181 cttaataagg ttgctgaatt tcacggagta aaggtactga atataaatca gctttctaat
      241 gcactcaaac ccatagtgtt gcctggtgaa gaaatggcag tatcccttat caaagaaggc
      301 aaagatccca atcaggcgat tggatactta gatgatggaa caatggttgt cgttgaaaat
      361 ggaagatcaa ggctcaattc caatgttgat gtaattgtaa catcggtttt acagacaact
      421 gcagggagaa tgatatttgc caggatcaaa gaagactgat gcaacaatag ttgcgttgtg
      481 tggaagcccc catatacagg gcaatactgc tactgcactg cggttattgt ttaatgaact
      541 atcgggtata tcaaaaagaa tatattttgc ctataagctg gctgttcagc cctgcactgg
      601 gtgtggatac tgtagcagga agtttggatg tatttttgac gatgcaatga atgaactata
      661 tgataccctg caacgtgctc atgtaatcat cattgcttca ccgttatatt tttcacacct
      721 tccttcaccg ttgaaatcca ttattgatcg ctgtcaggtt ttgtgggaat attataatcg
      781 gggtaataaa ccgctccacc tgcaatatgg gtttgcattg ttgcttggcg cagggaacta
      841 tagcaatatg ttcgtgccat cggtgattac attacgacat tattttaatt cacttaacta
      901 tcgctttgat gaaaaaaagt atttactgtt gtctgatata aataacaaaa aagatataag
      961 aaatgacgat attactaaaa agattactgc tgctgctaac tatattcaaa aattgttata
     1021 aaaagaggga agtatgaaat tatatgttac attgaccttt tcctgcacca gcgaggatct
     1081 ggcgttgttt gttaaaaagg ttgttgaatc aggatgttca atccgcagta ttaattatat
     1141 atcctcagaa ggaaattatg ctacctataa agctgatctt atgtatacag atagaaaaat
     1201 ttttaaggaa aaaattttaa gctgggtatc taaggaaacc acattgattg actgcagtga
     1261 tattatgtca aaaggcattg caggcggttt gctcaaggta tccagcaaga taccgtttga
     1321 ttcagctggt gaatttgagg gtaaaattct tggttatatt gaaatactta aagataatct
     1381 aattacggaa gaatcacgca aacttacagg gatgtccagc aattgtggta tgattattct
     1441 gacagaagat agtcaacaga tacagccaaa ggaaatatat cattatattc tgatagaaag
     1501 ggactcactg gtattgagtc attgcagcac actgaatcct cagccacttt taatgcgata
     1561 caggacaaaa gaggatatac tgaaaattgt agattccgtt tccactaatt atgctttgat
     1621 gcgaattgcc atgcctgata catttcagga agtttcactt tttcaccaga tggatgaaat
     1681 tcagagtctg gttttaagca gggaatatga tgagattccc ctttattgcc tcattaagat
     1741 tatgaatatt ttaaaacgcc atcatgtctc actggctgat tcagtaattg ggattgctgg
     1801 gattacaacc tcatccctgc gtttaactcg actgttatat gggattggat gcagcaaagt
     1861 actggggttt gatacaagtg aagtaaatct ttaccatttt gaaaaggaaa aaggtattgc
     1921 gacaaacatt gataatgttt ttgaaaatgc ggatgttgta ttatttatgt ctgattgttg
     1981 taccagtgaa gatttttata agttggggaa aggccagatt gtgttaatta atccagctat
     2041 acattttgat acgctggtat ttcaggaaag aggtttaaaa gattatgaat tactcattac
     2101 tgaaaattct tatctgctgc tacctggcct ggctgaaaaa atgatacagg gacagataca
     2161 ttccctaaat gatgagcaat tgatagccat aggtaaatca ttatatggag caaatcttga
     2221 tattgacagt gctgaaaata tgtacagtat tacagaaacc atacgaaaag tatcatagca
     2281 catttacgga tacagtatat accaggtaat attcttatat ttcaacaaac tgatattgta
     2341 ttttttccct gatatattaa ttgaagattt ttttaatgta tcttcatcaa gcttcaatgt
     2401 ccctcgttga attatgttaa atataacctc accgttagtt tcagcaatta ttgaatcagc
     2461 aggcaatatg tcataaaact tgttctgatt gatcagccca atacagaatc ccttgtttct
     2521 atcatctaat tgtataggat agcatacaca aaaactgtaa ttgcctgaca taggaggata
     2581 ctcaaagggt ttaaaaatag ctatatcatc atacggaaga tttgagacaa agtaatataa
     2641 cacgggagat agttgcgata atcttctgcc ataaagatca atgtttttca tggaataaag
     2701 tattgtataa gagctattga ttataatcag atcatcatag aattgttctg ttacattgct
     2761 tatcatctcg cttatatgct cgtattcaag cagataatac ttacgcccac cattatccat
     2821 gttcagaaga tgtgtgagat tggcaataag ttcatgtttt tgaataatag ttatagcatt
     2881 act
//
LOCUS       DAOSZP010000272         2882 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_27019_length_2882_cov_3.363990, whole genome shotgun sequence.
ACCESSION   DAOSZP010000272 DAOSZP010000000
VERSION     DAOSZP010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2882
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27019_length_2882_cov_3.363990"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1300
                     /locus_tag="PK348_06655"
     CDS             <1..1300
                     /locus_tag="PK348_06655"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HXK65929.1"
                     /translation="AFITFDMILPNQRDVLKQSGVTVIVTKITDFIDGFPKGSAQELQ
                     LEKNWHYFSDLLDSIDEASVPKIDISPDDPAIIVFTGGTTGAPKGAVHSHYNVVGATI
                     STMEVGKYFIDLMPAETRCNIGVMPFFHIAGHIQCFTWGIFRQTTMYLVPRFDINEFI
                     NLLARIPHKITYFFAVPTLLTALINHPKAKELALDKKFGLILSGGASCPVPLINKMKD
                     MGLFYIEAWGMTETCGIGISTPIFGIKKPGSIGIPMQESMIKIVDLEDGKTEVPMGKP
                     GELIFKSPFIMQEYWNNKEETAHVLKDGWLYTGDVVTMDEDGFIYIVDRKKDMIIAGG
                     YNVYPSEIDDVLLKHPKVDKAVTVGVPDTYRGETVKAFIVLKEGEVATEGEIIEYCKQ
                     HLAAYKVPKIIEFRKELPMSSVGKILRKVLREEEIKKVTK"
     gene            complement(1471..2079)
                     /locus_tag="PK348_06660"
     CDS             complement(1471..2079)
                     /locus_tag="PK348_06660"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HXK65930.1"
                     /translation="MKTKRIQYTMFNTPIVKHILRALAFILRTLLGWKKVGRLPDFPK
                     YLAILAFHTSNWDVFYGILIAFGFNVDIYFMAKNQLFRFPFAPLVRFLGGIPIDRSVS
                     SNTVQATIDMYNKHEKFAIALAPEGTRKKVEKWKTGFYHIATGAHIPIILAFLDYKTK
                     SGGIGAILHPTGDIKTDFKTIRDFYTQVHARYPELASLPQEE"
     gene            2253..>2882
                     /gene="nfi"
                     /locus_tag="PK348_06665"
     CDS             2253..>2882
                     /gene="nfi"
                     /locus_tag="PK348_06665"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583138.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HXK65931.1"
                     /translation="MNMHWNCSVEEAIAIQKELAGNVIIAGKPEKIDHIAGIDIALTP
                     DNKGYCIIAIFSFLNMELCEEVFCSDTINFPYVPGLLSFREGPLVLKAYEKLSIKPDV
                     MVFDGQGIAHPRRCGIASHIGVLLDIPSIGCAKSRLCGTYTEPGPDRGDRAYLYDKEE
                     IIGVVLRTRAHVKPVFISPGHKIGIDEAADIMMQCTGNYRIPVPTRYAHC"
BASE COUNT          919 a          459 c          622 g          882 t
ORIGIN      
        1 tgcatttatt acatttgata tgatattgcc aaaccaaagg gatgtgttaa agcaatctgg
       61 tgtaacagtc atagtaacca aaatcactga ttttatagat ggatttccaa aaggcagtgc
      121 tcaagaactt caacttgaaa agaactggca ttatttttct gatttactgg acagtattga
      181 tgaagcttct gttcctaaga ttgatataag tcctgatgat ccagctataa ttgtttttac
      241 tggtgggaca actggtgctc ctaaaggtgc ggtacattct cattacaatg ttgttggtgc
      301 tactatatct actatggaag ttggaaaata ttttattgat ttaatgccag ctgaaacacg
      361 gtgcaatatt ggtgtaatgc cgttttttca tattgcaggc catattcagt gtttcacctg
      421 gggtattttc cgacagacaa caatgtacct ggtgccaagg tttgatataa atgaatttat
      481 taatttgctg gcaagaattc cccataaaat cacttatttc tttgcggtgc caacattgct
      541 tacagcactt ataaatcatc ctaaagctaa ggagcttgcg cttgataaaa agtttggact
      601 tatactatcg ggtggtgctt catgtcctgt gccacttatc aataagatga aagatatggg
      661 attattttat attgaggcat ggggaatgac tgagacatgt ggtattggta taagtacacc
      721 aatatttggt ataaaaaagc ctggtagcat aggaattcct atgcaggaat caatgataaa
      781 gattgttgat ttagaagatg gaaaaactga agtaccaatg ggaaaaccag gtgagttaat
      841 atttaaaagc ccatttataa tgcaagaata ctggaataat aaagaggaaa ctgctcatgt
      901 actaaaagat ggatggctgt acacaggaga tgttgtgact atggatgagg atggatttat
      961 ctacatcgtt gatcggaaaa aagatatgat cattgcaggc gggtacaacg tatatccatc
     1021 tgagattgat gatgttcttt taaagcatcc aaaggtggat aaagctgtta cggttggtgt
     1081 tccagataca tacaggggcg agacagtaaa agcatttatt gttttaaaag agggagaagt
     1141 tgcaacagaa ggagaaatta tagaatattg caaacagcat ttagctgcat acaaggtacc
     1201 aaagattatt gagttcagaa aagaattacc aatgtcatct gttgggaaga ttttgcgtaa
     1261 agtgcttcgt gaagaagaaa taaaaaaagt tacaaagtaa aatcaacaag atattaagag
     1321 cgattaaaag agtcataata aaccccacct ggcagagtgg ggtttattat ataatattta
     1381 taaggttatg gcgttgcaaa taattgtatt attcattgtt taatactata gccaattata
     1441 aagatggcgt tatcaattgt attaacacag ttactcttct tgaggcaatg aagccaattc
     1501 gggataacgt gcgtgtactt gtgtgtaaaa atctctgatt gttttgaagt cagtttttat
     1561 atctccggtg ggatgtagta tggcacctat gcccccagat tttgttttgt aatctaaaaa
     1621 tgccagaatt attggaatat gtgcaccagt tgcaatatgg taaaaacctg ttttccattt
     1681 ttccactttt tttcgagtac cttcaggagc taatgctatt gcaaattttt catgcttatt
     1741 atacatatca attgttgcct gcacagtatt acttgatacg gagcgatcta taggtatgcc
     1801 acctaaaaaa cgaaccagcg gtgcaaacgg gaatcggaaa agttgatttt ttgccatgaa
     1861 atagatatca acattaaacc caaaggctat cagaataccg taaaatacat cccagttgga
     1921 agtatggaat gcaagtatgg ccaaatattt gggaaaatcc ggaagccttc ccactttttt
     1981 ccaacctagc aatgttctta aaataaatgc aagagcacga agtatatgtt taactatggg
     2041 ggtattgaac attgtatatt gaatacgctt tgttttcatg gtaactatcg ccctgaaaaa
     2101 attttggatg attcaatcat atggctaatt atatgacaat aatattttat gaggttgcaa
     2161 aaaattttgt agggtcagag ccaaagataa atgctcattt gtcattgaca tcagaatggg
     2221 gctatatata atcaaaaaat ggggtcagag caatgaatat gcattggaat tgcagtgttg
     2281 aggaagctat tgctatacaa aaagagctag ctggtaacgt tatcattgcg ggaaagcctg
     2341 aaaaaattga tcatattgca ggaattgaca tagctctgac cccggataac aaggggtatt
     2401 gcatcattgc aatcttcagt ttcctcaata tggaactttg cgaggaggtt ttttgctctg
     2461 acaccataaa ctttccgtat gttcccgggc ttctgtcttt cagagaaggg ccattagtgt
     2521 tgaaagctta tgaaaagtta tccataaaac ctgatgttat ggtttttgat gggcagggta
     2581 ttgcgcatcc ccgccgttgt ggcatagcca gccatattgg tgtgttgctt gatattccat
     2641 ctataggctg tgcaaaatca cgattgtgtg gtacatacac tgaaccagga ccagatagag
     2701 gtgacagagc atatctgtat gataaagagg agatcattgg tgtggtgctt cgcacacgtg
     2761 cacatgttaa gccggtattt atatcgccgg ggcacaagat tgggatagat gaagcagccg
     2821 atatcatgat gcagtgtacc ggtaactatc gtatacctgt acccacacgc tatgcgcact
     2881 gc
//
LOCUS       DAOSZP010000273         2876 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_27088_length_2876_cov_4.636299, whole genome shotgun sequence.
ACCESSION   DAOSZP010000273 DAOSZP010000000
VERSION     DAOSZP010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2876)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2876)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2876
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27088_length_2876_cov_4.636299"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /locus_tag="PK348_06670"
     CDS             <1..371
                     /locus_tag="PK348_06670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65932.1"
                     /translation="ILNIPKIPNPPVGGGGGGGGGGGGRLPLPPKPGDIPLMATGAVI
                     PPNASFLAILGDQKAGRNLEAPESLIRKIVREEAGGAQGQVITINFAGDLSALVRVLK
                     PYIDKENKRIGTSLVAGRVT"
     gene            368..739
                     /locus_tag="PK348_06675"
     CDS             368..739
                     /locus_tag="PK348_06675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65933.1"
                     /translation="MIRIDGETFNVPITSVSMSADMLDKYAERTNDGVLHRELIGVFY
                     NFELTFASSFQNAAEYARLWRKLSEPVDFHTVTLWDEDGEYTQVGYFAQTKHELIKLK
                     NGMPYWKSLSTSFVAKAPRSR"
     gene            769..2112
                     /locus_tag="PK348_06680"
     CDS             769..2112
                     /locus_tag="PK348_06680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65934.1"
                     /translation="MLGEVIEFRDRDIIEAKVTYEINPLSMELPASTAEVSIYTTNSD
                     FNPFSDAIYFQALKINTPADLVEYVNGTEIYIGRFYINEWNNPSIGVIKFYLQDAIGS
                     MDSIMFDGLFYEQPTNISTIVNVLNLQAPCDIVIDPAIYNVELKGYIPNGVSLRAALQ
                     QVCFAAGAYAKTQGIDHIFIKPARFADSGAVVIPAYYDNLSAIYDDINVRYSDIIVDD
                     VITDLEKLDKQELTILPIVTGIELTAHYYVKSDEAEVISDSLLVPGDYKIVYEKPYGD
                     VSVEGNVLALEYGTNSVSFNVPATTQVTITGYPYNDNTSKLIWVNPQSQQDYFEGALY
                     DAITSKYDDPNCLYGRLYSVNVAPNKWNITDATLIGDNNGQIVLNQLIKYAQEVYKQT
                     ITALADKNIDLGMLYLVDSLYDKKIVAGAEQVTINLSGGNLKETRLIGIEKMNYL"
     gene            2129..>2876
                     /locus_tag="PK348_06685"
     CDS             2129..>2876
                     /locus_tag="PK348_06685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65935.1"
                     /translation="MAATFPGGVKTFAVVNPGDPILSSTENAQQDEIKAIETELLKSS
                     GSVVNHDALTNFVANEHIDHSSVSITVGTGLSGGGDLTTSRTITLSHLGLQNLTDPGA
                     DRIMFWDQSAGMLKWLTVSTGLAITDTSLAFSHLGLQNLTSPGEDRVLFWDQSAGMFK
                     WLTLGTGLTINDTTIEFSDSTVLQNLIYIQLFGKDEVITTTSAKAYLFVPSYLSGKKI
                     KKMGMGIVTAGTSDTTVALDTYASVSGNGYT"
BASE COUNT          901 a          470 c          633 g          872 t
ORIGIN      
        1 gaattctcaa cattcctaaa attcctaatc cgcccgttgg cggcggtggt ggcggtggtg
       61 gcggtggtgg tggacgcctt ccgttaccgc caaagcctgg tgatattcca ctgatggcta
      121 ctggtgcggt tatacctccc aatgcatcct ttttagctat acttggagat caaaaagcag
      181 gacgcaattt ggaagcgcca gagagcttaa ttagaaaaat tgtgcgtgag gaagctggtg
      241 gagcacaagg tcaggtaatt acgattaatt ttgctggtga tttgagtgct cttgtgcgag
      301 tgctcaaacc atacattgat aaagaaaaca agcgtatagg tacgagttta gttgctgggc
      361 gggtaacatg attagaatag atggtgaaac ttttaatgtg cctattacaa gtgtgtcgat
      421 gtctgcagac atgttggata aatatgcgga gcgcacaaat gatggcgtgt tacatcgaga
      481 gctaattggg gttttttata actttgaact aacctttgct tcgagctttc aaaacgcagc
      541 tgagtatgca aggttgtggc gcaaattgtc tgaacctgta gatttccaca ctgttacatt
      601 atgggacgag gatggcgagt atactcaggt aggatatttt gcacaaacca aacatgagct
      661 tatcaagtta aaaaatggta tgccgtactg gaaatctctt agtacctcat ttgtggcaaa
      721 ggcgcctcgg agcagatgat gataacctac cccatactca ggctgtattt gcttggtgaa
      781 gtaattgagt ttagagacag ggatatcatc gaagcaaaag ttacttacga aattaacccg
      841 ctcagtatgg agcttcctgc gtcaactgct gaagttagta tatatacaac taatagcgat
      901 ttcaatcctt ttagcgatgc tatttatttt caagcgctca aaataaacac accagcagat
      961 ttagttgagt atgttaatgg tacagaaata tatattggca gattttatat taatgagtgg
     1021 aataatccgt ctattggtgt aattaagttt tatttgcaag atgcaatagg ctcaatggat
     1081 agtatcatgt ttgatggttt attctatgag cagccaacta atatttctac gattgtaaat
     1141 gttttaaatc tccaagcgcc ttgtgacata gtaatagacc cagctattta caatgttgaa
     1201 ttaaaaggct acataccaaa tggtgttagt ttgcgtgcag ctttgcaaca agtgtgtttt
     1261 gcggctggag cttatgctaa gacgcaaggt attgaccaca tatttattaa gcctgcgaga
     1321 tttgcagatt caggggcagt agtaatacca gcatattatg acaatttgag tgcaatttat
     1381 gatgatatta atgtgcgcta ttctgatatt attgttgacg atgttattac cgatttggaa
     1441 aagttggata aacaagagct tacaattctt ccgattgtaa ctggaattga acttacagct
     1501 cattattatg taaaatctga tgaagctgaa gtaatatccg atagtttgct tgtgccaggt
     1561 gattacaaga ttgtatatga aaagccatac ggtgatgtgt ctgttgaagg caatgtttta
     1621 gccctggaat atgggacaaa ttctgttagt ttcaatgttc ctgctactac acaagtgacc
     1681 ataactggat atccatacaa tgataatacc tcaaaactta tatgggtaaa tccacaatca
     1741 caacaggatt atttcgaagg cgctctgtat gatgcaatta cgagcaaata tgatgatcca
     1801 aattgtcttt atggtcggct atactcagta aatgttgctc ccaataaatg gaatataaca
     1861 gatgcaacat taatcggaga caataatggg caaatagtat tgaatcaact tataaaatat
     1921 gctcaagaag tatataagca aacaattacg gcgcttgcag ataagaatat tgacttgggt
     1981 atgttatatt tagttgatag cctatacgac aaaaaaattg tggcaggagc agaacaggtc
     2041 acgattaatc tttctggtgg taaccttaaa gaaactcgtt tgataggcat agaaaaaatg
     2101 aattatttat gatagatagg agcaatatat ggcagcaaca tttcctggtg gagtaaaaac
     2161 ctttgcagta gtaaatcctg gtgacccgat tctctcctct actgagaacg cacagcagga
     2221 tgaaataaaa gcaatagaaa cagaactatt gaaatcaagt ggttctgtgg taaatcatga
     2281 tgcactgact aattttgtag ctaatgagca tattgatcat agttcagttt caataacagt
     2341 gggaactggt ttgagcggtg gtggagattt aactacttca cgtactataa cgctttctca
     2401 tttaggattg caaaatttga cagacccagg tgcagatcgc ataatgtttt gggatcaaag
     2461 tgctggaatg cttaagtggt tgacagtaag tactgggtta gctataacag acacctcatt
     2521 agcattttct catttaggat tgcaaaactt aacaagtcct ggtgaagacc gagttctatt
     2581 ttgggatcaa agtgctggaa tgttcaaatg gttaacctta ggcactggat taacaataaa
     2641 tgatactaca atagagtttt cagattcaac tgttttgcaa aatcttatat acattcaact
     2701 ttttggtaaa gatgaggtta ttacaacaac ttcagcaaaa gcttatcttt ttgtaccatc
     2761 atatctttct ggaaagaaaa taaagaaaat gggtatgggg attgtaacag cagggacgag
     2821 tgatacgaca gttgcgcttg acacttatgc aagtgtttct ggtaatggct atacag
//
LOCUS       DAOSZP010000274         2876 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_27092_length_2876_cov_3.064162, whole genome shotgun sequence.
ACCESSION   DAOSZP010000274 DAOSZP010000000
VERSION     DAOSZP010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2876)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2876)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2876
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27092_length_2876_cov_3.064162"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..626
                     /locus_tag="PK348_06690"
     CDS             <1..626
                     /locus_tag="PK348_06690"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HXK65936.1"
                     /translation="LAYTEKSKFAQAIATNWINALLRYDIICTRSDPLRDPCGYRTLL
                     VWKLASQYYKNTELEKIFAMRSPLAYMRPKEIDCIALLETGACDAMWIYKSVAVQQNF
                     PYITLDEHINLGSHTYEHIYRKACITLSDTKKQYTFCGSTITYGASIPVTANNVKGAI
                     AFLSFLCSKEGQGLINKHGFTSIVPYSNNSEQLPVELKKVVAGGNRK"
     gene            610..1413
                     /locus_tag="PK348_06695"
     CDS             610..1413
                     /locus_tag="PK348_06695"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXK65937.1"
                     /translation="MVTVNKKNIVFYSIVVVLSFVVAFLVVVPIASMVLSVSVRDVVD
                     ILFDREVVQSLLLTFECAFYATLIAVLFGIPLAYLFAFTHFPLKRLIITLFDIPMIIP
                     HTASGIALLFVFGSGFLSHLFKSLHMSFVGSKTGIVIAMAFVSIPYFINAVQNGFASI
                     DRRLINVARTLGASQTQCLVHIILPLSVRAIITGSLMMWGRGISEFGAVMIIAYHPMI
                     APVLLYDRYSAYGLEGSRPIAALLIVFCIVFFILIRSLLAYREKHAYTA"
     gene            1397..2446
                     /locus_tag="PK348_06700"
     CDS             1397..2446
                     /locus_tag="PK348_06700"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXK65938.1"
                     /translation="MLTLHNIQWESKQFTLAIPSLQFHDGAHAMIMGSSGSGKTLLLS
                     IIAGIIAPHHGTIYSDNEDITHIPPETRGFSIVYQEPALFAHMTVFENIAFGLRMRKY
                     HARIITQKVNEVMELCGVHHLQHRNPATLSGGEKQRVAFARAIVNAPKLLLLDEPFTA
                     LDPVSKGELIGILNHIKKEYNPTVLHVTHDFDETLQLGNYLYIMSKGAIIQHGTVTDV
                     YRYPSDIFVAQMVGVKNIFHGSVVHKKDGAYFVTDNGIAFFLGDSYRKFFQYAILRAE
                     DIIISRKLLHSSATNQFKGTVIDVTEVKGLYKVSVDIGEIVQSMITKRSLQAMNIKKG
                     AKVVVIFKGSAIHLF"
     gene            2668..>2876
                     /locus_tag="PK348_06705"
     CDS             2668..>2876
                     /locus_tag="PK348_06705"
                     /inference="COORDINATES: protein motif:HMM:NF012725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXK65939.1"
                     /translation="MKRLFYSFMVTTILCFISSVTIAQHIEEAVQLNTKGLFYLECGN
                     YEKAYEYFMLALQHDPTNKLLYNNCA"
BASE COUNT          861 a          526 c          597 g          892 t
ORIGIN      
        1 tgctagcata tactgaaaag agtaaatttg cacaggcaat agcaacaaac tggataaatg
       61 cacttttacg atatgatatt atctgtacac gctctgaccc cttacgggac ccctgtggat
      121 acagaacact tctggtatgg aaattggcat cacaatatta taaaaataca gagcttgaaa
      181 aaatctttgc catgcgttca cctcttgcgt atatgcgccc aaaagaaatt gattgcattg
      241 cattgcttga aacaggcgcc tgtgacgcga tgtggatata caaatcagtt gcggtacagc
      301 aaaatttccc ctatataacg ctggatgagc atataaattt aggcagtcat acatacgagc
      361 atatatacag gaaagcctgt attacgctgt cagacacaaa gaagcagtat acgttttgcg
      421 gaagcaccat cacctatggt gcatccattc cagttactgc aaacaatgta aaaggtgcta
      481 ttgccttttt atcgtttctt tgtagcaaag aaggacaggg attaataaat aagcacggat
      541 ttacatctat agtaccgtat agcaataata gtgaacaatt acctgtggaa cttaagaagg
      601 tggttgcagg tggtaaccgt aaataaaaaa aatattgtgt tttacagtat agttgttgtg
      661 ctgtcgtttg tggtagcatt ccttgttgtg gtgcccattg cttcaatggt gctttcagta
      721 tcagtacgtg atgttgtaga catacttttt gatagggaag ttgtgcaatc gcttctttta
      781 acctttgaat gtgcattcta tgctacgttg attgcagtac tttttggcat accccttgcc
      841 tatctctttg cctttactca ttttccgttg aagcgtttaa ttatcacgct atttgatata
      901 cccatgatca ttccccatac cgcaagcggc atagcattac tttttgtatt tggaagtggc
      961 tttctatcac atttatttaa gtcgctacac atgtcatttg ttggtagcaa aacaggcatt
     1021 gtcatagcca tggcttttgt ttcaataccg tattttatca atgcagtgca aaatggtttt
     1081 gcctcaattg acaggcgcct tattaatgta gcacgaacgt taggtgcttc acagacgcag
     1141 tgccttgtcc atattatatt accactttca gtcagggcta tcattaccgg tagtcttatg
     1201 atgtggggac gaggcatcag cgagtttggc gctgttatga ttattgctta ccaccccatg
     1261 attgcacctg tccttttgta tgacaggtac tcagcatatg gattggaagg ttcacggcca
     1321 attgctgcac tgctcatagt tttttgcata gtgtttttta ttcttatacg gagtttactt
     1381 gcttacaggg aaaaacatgc ttacactgca taacatacaa tgggaaagta aacagtttac
     1441 gctggcaatt ccatcattgc aatttcatga tggagctcat gccatgatca tgggctcttc
     1501 aggctcaggg aagacgttac tgttgtccat catagcagga attattgctc cccatcatgg
     1561 caccatctat agtgacaatg aagacattac gcatatcccc cctgaaacac gtggcttttc
     1621 cattgtgtat caggaaccag cgttatttgc ccacatgaca gtttttgaaa acatagcgtt
     1681 tggattgcgc atgaggaagt atcatgcccg aataataacg caaaaggtta atgaagtgat
     1741 ggagctatgc ggtgtgcacc atttacaaca cagaaaccca gcaacgttaa gtggtggtga
     1801 aaaacaacgc gtggcatttg cgcgagccat agtgaatgca ccaaaacttt tattgcttga
     1861 tgagccattt accgctcttg acccggtatc caaaggggaa ctcattggta ttttgaacca
     1921 cataaaaaaa gaatataatc ctacagtgct gcatgtgacg catgattttg atgaaacgct
     1981 gcagcttggc aattacctgt atatcatgag taaaggtgca atcatccagc atggtactgt
     2041 taccgatgtg tatcgctatc ccagtgatat ttttgtagca cagatggttg gggtaaaaaa
     2101 tatttttcat ggaagcgttg tacataaaaa agatggtgcg tattttgtaa cggataacgg
     2161 cattgcattt tttttaggcg atagttatcg caaatttttc cagtatgcaa tactcagggc
     2221 tgaggatata atcatatcca ggaagctgtt gcatagcagt gcaaccaatc agtttaaagg
     2281 aactgttata gatgttactg aggtgaaggg cttatataaa gtaagcgttg atataggtga
     2341 aatagtacaa tcaatgatca ccaaacgatc attacaagca atgaacataa aaaaaggtgc
     2401 aaaagttgtt gtaattttta agggcagtgc tatacacttg ttttaaatgt gatttgaatt
     2461 atattgatac tatcaaaagc aatcttgtta attgtagtga atgtacgata tcctaattgt
     2521 gaatagatga catagcagcg agtgtcaggc atgcttcaac aggttcagca gatgatttga
     2581 tgaacatgtt gcgatagttg tagtacttag tcaaagacta acgatataat ttttcaaaat
     2641 tataatagca ttaccacgag ggatgaattg aagagattat tttatagctt tatggttact
     2701 acaatattgt gctttatttc atcggtaact atcgcacagc atatagaaga agctgtacag
     2761 ctcaatacca aaggtctttt ttacttagaa tgcggtaatt atgaaaaggc gtatgaatat
     2821 tttatgctgg cactgcagca cgatccaacc aataagttat tatataataa ttgcgc
//
LOCUS       DAOSZP010000275         2863 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_27231_length_2863_cov_2.924145, whole genome shotgun sequence.
ACCESSION   DAOSZP010000275 DAOSZP010000000
VERSION     DAOSZP010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2863
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27231_length_2863_cov_2.924145"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1028)
                     /locus_tag="PK348_06710"
     CDS             complement(<1..1028)
                     /locus_tag="PK348_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010569722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HXK65940.1"
                     /translation="MIKKIISFSVHNKALVLFVISFLLLYAVYAIENIPLDAIPDLSD
                     TQVIIYTRWDRSPQIVEDQVTYPIVSALLGAPNVKTIRGFSDYGFSYIYVIFEDGTDI
                     YWARSRVVEYLSKIQGSLPQDVRMELGPDATGVGWIYQYALIDKSGKYDLSQLRTLQD
                     WYIKYSLQSVPGVAEVATVGGFEKQLQVTINPNTLHSYNISLLDIIEAIRKSTNEMGG
                     RLIEWSGKEYMVRAEGYIENINALKKVVVKYNIPTPVLLEHVADVTVGPQIRRGIAEF
                     NGEGEVVGGIIVMRHKENALNVIKKVKEKIQEVKKGLPEGVEIVPVYDRSGLIKQSIS
                     TLTDALIE"
     gene            complement(1051..2307)
                     /locus_tag="PK348_06715"
     CDS             complement(1051..2307)
                     /locus_tag="PK348_06715"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HXK65941.1"
                     /translation="MKKLLIGVIIVSAIAATIYVISNYRQNIPDKNASNVTYTCPMHP
                     QITSDKPGSCPICGMDLVPVEEEKKNESHKEHQEIAGYTSILLDEKARILGITYETVG
                     IKDIVKILRTSATIVANEEKTYKISFRISGWVDKLFVNKMGQFISKGEPLLSLYSPEL
                     YAAVSEYISIINSIETMSNNQTVADTLVNLKKSSKEKLLLYGLTEKQVNEIETKKEAT
                     RTVTIYSPYTGYIIDKNVFEGQKVNSNEILMTIVDLTTVWAIAEVYQPDLLYIKPGMP
                     ATLSISYWGNKEYRGNVQFVYPFVNEQTRTVKVRAVFYNNALELKPGLYGEIMLRYSA
                     GKHLAVSEQAVFKTGEKNYVFVKHGDDTLMPQEVVLGALGDNGYYEVQKGVRKGDVIV
                     SPANFLIDSESQLKAVFSNVKEGHVH"
     gene            complement(2304..>2863)
                     /locus_tag="PK348_06720"
     CDS             complement(2304..>2863)
                     /locus_tag="PK348_06720"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HXK65942.1"
                     /translation="PELQLLSIQVLIDEEAANLKENELYPDVEVGVSYMQRDKTPQGV
                     NRDDMFSVMATFNIPVWFMSKNIPAIQEMKIKKGESEALLKDKQNEITFALATLRQNI
                     EKWKQLDELYSQGVIPQLHSMLQADIANYRTGVAEFMKLIDSIRMMLHYKQQQLNTIK
                     EYCKAVSFLHFLLGDITIQRWYDAQ"
BASE COUNT          833 a          595 c          433 g         1002 t
ORIGIN      
        1 tcttctataa gtgcgtctgt taatgtagaa attgattgtt taattagacc tgatctgtca
       61 tacacaggaa ctatctccac cccttcgggt aagcctttct taacttcttg aattttttct
      121 tttacttttt tgataacatt aagggcattt tctttatggc gcattacaat gattccccca
      181 acaacttcac cctctccatt gaattcagca atacctctac gaatctgtgg ccctacagta
      241 acatcagcaa catgctccaa taaaacaggt gtagggatat tgtatttaac cacgactttt
      301 tttaaggcat taatgttttc tatatatcct tcagctctca ccatatattc tttgccagac
      361 cattcaatga ggcgaccacc catttcattt gtgcttttcc ttattgcttc tatgatatca
      421 agtaaactta tattataact atgtaatgta tttggattaa tagtaacctg taattgcttt
      481 tcaaaaccac caactgttgc aacttcagca actccaggga ccgattgcaa tgaatactta
      541 atataccagt cctgtaatgt tcttaattgc gaaagatcat atttgccgct tttatcaatc
      601 aatgcatact gataaatcca acccacccct gttgcatccg gtcccagttc cattctaaca
      661 tcctgtggca atgaaccctg aatttttgaa agatactcaa ccactctgct tcgtgcccag
      721 taaatatcgg tgccatcttc aaaaataaca taaatataag aaaatccgta gtcactgaac
      781 ccacggattg ttttaacgtt tggtgctccc aataaagctg agacaattgg atatgttacc
      841 tgatcttcaa ctatctgggg gcttctgtcc cacctggtat aaattatcac ctgggtatca
      901 gataaatcag gaatagcatc aaggggaatg ttttcaatag catagacagc atataacagt
      961 aaaaacgata taacaaataa taccagtgct ttattatgaa cagaaaatga tataattttt
     1021 ttaatcatac atccttacca tggtttatca tcaatgcaca tggccttcct ttacattact
     1081 gaatacagct tttaattgcg attcagaatc aatcaggaaa tttgcaggtg acacaataac
     1141 atcacctttc ctgacccctt tttgtacttc ataatatcca ttgtcaccca gagctcctaa
     1201 tacaacttct tgcggcatca atgtgtcatc accatgtttt acaaaaacat agtttttttc
     1261 tcctgtttta aacacagcct gttctgatac agccagatgt tttcccgcag aatatcgtaa
     1321 cataatttca ccatacaatc ctggtttaag ctctaatgcg ttattgtaaa atactgccct
     1381 tacttttact gtacgagttt gttcattgac aaaaggatac acaaattgca catttcctct
     1441 atactcttta ttaccccagt acgatatgct caacgtagct ggcattcccg gttttatata
     1501 tagcaaatcg ggctgataca cctctgcaat tgcccacact gttgtaaggt caacaatcgt
     1561 cattaaaatt tcattggaat ttactttttg accctcaaaa acatttttat ctataatgta
     1621 acctgtatat ggtgaatata ttgtgacagt tcttgtggct tcttttttag tttctatttc
     1681 attaacctgt ttttcggtaa ggccatagag taaaagtttt tctttagatg attttttaag
     1741 atttactagc gtatctgcta cagtttgatt attggacatc gtttcaatag aattaataat
     1801 tgaaatatat tcactgaccg ctgcatacag ttcaggactg taaagcgaaa gcaatggttc
     1861 tccttttgaa atgaactgcc ccattttatt aacaaataat ttatcaaccc accctgaaat
     1921 tctgaaacta attttatatg ttttttcttc atttgccact atggttgccg atgtccgcaa
     1981 tatttttaca atgtctttaa tgccaactgt ttcatatgtt atgccaagta tacgtgcctt
     2041 ttcatcaagc aatatggagg tataccctgc aatttcctga tgttccttat gtgactcatt
     2101 ctttttttct tcttcaactg gaacaaggtc cataccacat attggacaac taccaggttt
     2161 atccgaagta atttgtggat gcattgggca ggtataggta acgtttgaag catttttatc
     2221 aggtatattt tggcgatagt tactgataac atatattgtt gcagcaatcg cgctaacgat
     2281 tataacacca atcagtaatt ttttcattgt gcatcatacc acctttgtat cgttatatca
     2341 cccagtaaaa aatgtaaaaa tgaaaccgct ttacaatatt ctttaatcgt gttcagttgc
     2401 tgctgtttat aatgcaacat catgcgaatg ctatcaatta atttcataaa ttcagcaact
     2461 cccgttctat agttagctat atcagcctgc agcattgaat gcagttgtgg tataacacct
     2521 tgtgaataca gctcatcaag ctgcttccat ttttcaatat tttgccgtaa tgttgcaagt
     2581 gcaaaagtga tttcattttg tttatctttg agtagcgctt ctgactcacc ttttttaatc
     2641 ttcatctcct ggatagcggg aatgtttttg ctcatgaacc atacgggtat gttaaacgtt
     2701 gccatgacac tgaacatgtc atcacggttt acaccctgcg gagttttatc acgctgcata
     2761 tatgaaacac caacttctac atcaggatat aattcgtttt ctttaaggtt tgcagcttct
     2821 tcatcaataa gtacttgaat agatagtaac tgtaattctg gat
//
LOCUS       DAOSZP010000276         2848 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_27411_length_2848_cov_3.548514, whole genome shotgun sequence.
ACCESSION   DAOSZP010000276 DAOSZP010000000
VERSION     DAOSZP010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2848
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27411_length_2848_cov_3.548514"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            145..1629
                     /gene="lysS"
                     /locus_tag="PK348_06725"
     CDS             145..1629
                     /gene="lysS"
                     /locus_tag="PK348_06725"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HXK65943.1"
                     /translation="MSEDINDLIVQRIEKVKALRAKGINPYPIRFKRTHTAVQIKELF
                     TEGQELQVKAAGRIKSKRVMGKASFAHIEDLSGLIQVYARLDRMGDEQYELFKTLDLG
                     DIIGLSGHVFKTKQGEITIEISDVVLLAKCIRPLPVVKEKDGRVFDEFADKEQRYRQR
                     YVDLIVNTQVRQQFILRSRLITGIRTFLNQKDFIEVETPMMQAIPGGAAARPFITHHN
                     ALDIDLYLRIAPELYLKRLLVGGFEKVYELNRNFRNEGISTRHNPEFTMLELYQAYAD
                     YNDMMDITQEMISTLAQQLLGSMVIQYQGNTIDLTPPWQKISYVDALKKYTGINFQEL
                     SHSEALRKAKEMGLDVDESMSKWKIAEEMFEEKVEANIIQPTFIIDYPKELSPLAKSR
                     DDNPEFVERFEVYIAGREMANAFSELNDPFDQRERFEQQVKMREAGDDEAQMMDIDYV
                     TALEYGMPPAGGLGIGIDRLAMLFIDTASIKDTILFPLLKPENR"
     gene            1635..2279
                     /locus_tag="PK348_06730"
     CDS             1635..2279
                     /locus_tag="PK348_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015403863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HXK65944.1"
                     /translation="MVAHYSYKQYGLHIHRFLYDWVLSWAHTPFGERALFVLALAESS
                     FFPVPPDVLLIALVLGKREAFVRLATLCSIASVIGGLVGYGIGYFLWYDGDSYSKIAL
                     FFFNNIPGFTVETFTAARQLYDAYSFWIVFTAGFTPIPYKVITITAGVANINIFIFVI
                     ASMISRTLRFFLVAGLIWKFGEPINAFINKWFNVLSIVFVILLFAGFYVLKYMV"
     gene            2307..>2848
                     /locus_tag="PK348_06735"
     CDS             2307..>2848
                     /locus_tag="PK348_06735"
                     /inference="COORDINATES: protein motif:HMM:NF013116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HXK65945.1"
                     /translation="MKIFIDSADIDEIAKAYEWGIVDGVTTNPSLLKTAVDRRKAKGE
                     KLDLEAYIVKLLEISKPNPVSLEVTKRTAQEMIEQGRNLYKRFNSIANNVNIKIPVNP
                     AMKSDEQCHFDGINAVKVLQSEGIPVNCTLVFTPEQALLAAKAGAAYISPFAGRIDDL
                     IRSRANIKFGKSDYFPEEGM"
BASE COUNT          833 a          488 c          691 g          836 t
ORIGIN      
        1 tgtaatattt tacaaaaaat ttgttgaaaa gagtggttgc aggcaaagaa aagtattatt
       61 gcagtaacta tcgcttgaca taaaaagatg aaaatatatg gttttataga tataatacta
      121 tttatttcca ggggataaca ggtaatgagt gaagacatta atgatttgat tgtacagcgc
      181 attgaaaagg taaaagctct tcgtgcaaaa ggtataaatc cgtatccaat acgatttaaa
      241 cgaactcata cagcagtgca gattaaagag ttatttactg aagggcagga attgcaggta
      301 aaggctgctg gcaggataaa gtccaaacgc gttatgggca aggcaagctt tgcgcatatt
      361 gaagatttaa gcgggcttat ccaggtatat gcgcgccttg acaggatggg tgatgaacaa
      421 tatgaattgt ttaagacgtt ggaccttggc gatattattg gcctatcggg tcatgtgttt
      481 aaaaccaaac agggtgaaat aaccattgaa attagcgatg tagtgttgct tgctaaatgt
      541 atacggccgc ttcctgttgt taaggaaaag gatggcaggg tttttgacga atttgcggat
      601 aaagagcagc gatacaggca gcgctacgtg gatttgattg tcaatacaca ggtgcgccag
      661 cagtttattc tcagaagcag gctcataact ggtatacgga cgtttttaaa tcaaaaagat
      721 tttattgaag tggaaacacc aatgatgcag gccatacccg gtggtgcggc ggctcgtccg
      781 tttattactc atcacaatgc actggatatt gacctgtact tacgcattgc gccggagtta
      841 tatttaaaaa ggcttctggt tggtggattt gagaaggtgt atgagttaaa tcgcaatttc
      901 cgcaatgaag gaatatcaac ccgccataat ccagagttca ccatgcttga attgtaccag
      961 gcgtatgcgg attacaacga tatgatggac ataacccagg agatgataag tacactggct
     1021 cagcagttac tgggtagtat ggtcatccag tatcagggta acaccattga cctcacgccg
     1081 ccgtggcaaa agatttcata tgttgatgcg ctgaaaaaat acactggcat taatttccag
     1141 gaactatcgc acagtgaagc gttaagaaaa gcaaaggaaa tgggcctgga tgttgatgaa
     1201 agcatgtcta agtggaaaat agcagaagag atgtttgaag aaaaggttga ggctaatatc
     1261 atacaaccta cttttatcat agactatccc aaggaactat cgccccttgc aaaaagcagg
     1321 gatgataatc ctgagtttgt tgagcgcttt gaggtatata ttgcaggaag ggaaatggct
     1381 aatgcattca gtgaattaaa tgacccattt gaccagcgtg agcgctttga acagcaggta
     1441 aagatgcgcg aggctggcga tgatgaggca cagatgatgg acattgatta cgttacggcg
     1501 cttgaatatg gtatgccacc ggcaggtggc cttggcattg gcatagacag gctggccatg
     1561 cttttcattg atactgcgtc aattaaggat accattttgt tcccgctgtt aaaaccagaa
     1621 aaccggtagt gtatatggtt gcccattatt cttataaaca gtatggattg catattcaca
     1681 ggtttttata tgactgggtc ctttcgtggg cgcatacacc atttggtgag cgtgcattat
     1741 ttgtacttgc actggccgag tcgtcttttt tcccggttcc gcctgatgtg ctgctcattg
     1801 cgctggtact tggcaaacga gaggcatttg tgcggcttgc aacactgtgc tcgattgctt
     1861 cagtaattgg ggggcttgtc gggtatggta ttggatattt tttatggtat gatggcgata
     1921 gttattcaaa aatagcccta ttctttttta ataatatccc ggggtttacg gttgaaacat
     1981 ttactgctgc ccgtcagcta tatgatgcct attcattctg gatagtgttt actgccgggt
     2041 ttacgcctat tccgtataaa gtcataacca tcaccgcagg tgttgccaat atcaatatat
     2101 ttatatttgt aatagcttcg atgatcagcc gtacactgag gtttttcctt gttgccgggc
     2161 ttatatggaa atttggcgaa ccaattaatg cgtttattaa taaatggttt aatgtattat
     2221 ctatagtgtt tgttatcctg ctatttgcag gtttttatgt gttaaagtat atggtttaga
     2281 cattttagat ttaatggagg attggtatga agatttttat tgatagtgcg gatattgatg
     2341 agatagcaaa ggcgtatgaa tggggtattg ttgatggcgt aacaactaac ccttcgttgc
     2401 ttaagactgc tgttgataga agaaaagcta aaggcgaaaa gcttgacctg gaagcatata
     2461 ttgtaaagct tttagaaata tcaaaaccca acccggtgag tttagaagta acaaagcgca
     2521 ctgcccagga aatgatagag cagggacgca acctgtataa gcgcttcaac agtattgcaa
     2581 acaatgttaa tataaaaatt ccggttaatc ctgccatgaa atctgatgag cagtgtcact
     2641 ttgatggaat taatgcagta aaagtgttac agtcggaggg cattccggtt aactgtactc
     2701 ttgtctttac tcctgaacag gcattactgg cagcaaaggc tggggcggca tatatcagtc
     2761 catttgctgg ccgtattgat gaccttatcc gcagcagggc aaatatcaaa tttggtaaaa
     2821 gtgattattt ccctgaggaa ggcatgga
//
LOCUS       DAOSZP010000277         2841 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_27493_length_2841_cov_2.951902, whole genome shotgun sequence.
ACCESSION   DAOSZP010000277 DAOSZP010000000
VERSION     DAOSZP010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2841)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2841)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2841
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27493_length_2841_cov_2.951902"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..456)
                     /locus_tag="PK348_06740"
     CDS             complement(<1..456)
                     /locus_tag="PK348_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009172365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HXK65946.1"
                     /translation="MISIAIVVVSDRSYRKEREDLSGKAIEEWARSRKYSVVEFCIVP
                     DEREVIAQTLRDLCAKNINCILTTGGTGFAPRDVTPEATADVIERYAPGFAEAMRHAS
                     LAITKHAMLSRAIAGIRGSSIIINLPGSPKAVVENLEVIQDALPHAVKLL"
     gene            complement(458..808)
                     /gene="hypA"
                     /locus_tag="PK348_06745"
     CDS             complement(458..808)
                     /gene="hypA"
                     /locus_tag="PK348_06745"
                     /inference="COORDINATES: protein motif:HMM:TIGR00100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HXK65947.1"
                     /translation="MHELSIMSNVLDIVVQHASQNGASKVTKIHLLVGELSDYIPEWM
                     QTYFDFVSKDTIAEKAELVVEMVKASLKCDDCGNEFHFNKNDWQFSCPQCNSPNVTII
                     SGRELTVKSIEIEG"
     gene            901..2658
                     /locus_tag="PK348_06750"
     CDS             901..2658
                     /locus_tag="PK348_06750"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HXK65948.1"
                     /translation="MQKKIDSYITHNIHYIDFCIGQLKKDIDVLPNRTNAITEILEHI
                     LFQKKLTLPLSNEGITACNDLARVLTQWDIIQHGDLAGYIYQNIKDTDDKKKKGQYFT
                     PPAIVQYMVEHALQYHDNPFTVKILDPACGSGQFLIYIYQYLLELYKQHGISHEDAAY
                     TICQNNLFGYDIDPVACMIAQWNIQKLSGTHTNIFHRDMLSTDDLFNKHNSLVFDIII
                     GNPPWGAHFTHEQKQYFKQHYFSAISGINSFTLFIERSLSLLHERGILSFLIPEAYLN
                     IKAHQNSRQIVLSNTVIKEIVQWGDCFKGVYAPAISLTAQKEEREEIRKKHIVHIKHA
                     KSLTTIAHMIPQQMYCTTHESIFPINYTRKAVALLEHIHTLSYITLQDRVKFFLGIVT
                     GNNDYHISTIKDSRHPDPIIIGKDVEKFRINFSNHYFKYNVSTLQQVAEQQCYLTRNK
                     ILYKFIGRHLTFAVDPHGSYTLNNVNGFIPLSPQLDPDVLVAILNSRLLQYIYETQFF
                     TVKVLRGNLEKLPIKIINSTNKKLMKKLSLELREEADRVKRKILMDTIDDIVFHEYRI
                     NDMLAHHIHESITTGISIA"
BASE COUNT          938 a          604 c          430 g          869 t
ORIGIN      
        1 taaaagtttg acagcatgcg gtagtgcatc ctgtataacc tctaaatttt ccacaacagc
       61 ttttgggctt cctggtagat taataataat tgaagagccc ctgatacccg ctattgcccg
      121 tgacagcata gcatgtttgg ttattgcaag ggaagcatgg cgcatggctt ccgcaaatcc
      181 tggagcatag cgttcaatca catcagcggt tgcctcagga gtgacatcgc ggggagcaaa
      241 ccctgttcct cctgttgtta aaatacaatt aatattcttt gcacacaggt cccgtagcgt
      301 ttgtgcaatc acttcgcgct catcaggaac aatacaaaat tcaaccacac tatatttcct
      361 gctcctggcc cactcttcta ttgccttgcc agaaaggtcc tcgcgctctt ttcggtagga
      421 cctgtcactt accacaacta tcgcaatact tatcatacta tccctctatc tctatacttt
      481 ttacagtaag ctccctacct gaaatgatgg ttacattagg agaattacat tgtgggcaac
      541 taaattgcca gtcattttta ttaaagtgaa actcattgcc gcaatcatca catttcagtg
      601 atgccttcac catttcaaca accagttcag ctttctccgc aatggtatcc ttacttacaa
      661 aatcaaaata tgtctgcatc cattcaggaa tataatcaga caattccccc accagaagat
      721 gtattttagt taccttgctt gcaccatttt gtgatgcatg ctgaacaaca atatcaagga
      781 cattggacat gatggataat tcgtgcattg tttcctcaca aacgctaatc gtttttatac
      841 aatatatcga tatgtaaata tgaacaatct attctacatt ttttgtaacc gatagttact
      901 atgcaaaaaa aaattgattc atatattacc cataacattc attatatcga tttctgcatc
      961 gggcaactaa aaaaagatat tgatgtgtta cccaatagaa ccaatgccat tacagaaata
     1021 ctggaacata ttcttttcca gaaaaaacta acactcccat tatcaaatga gggaatcaca
     1081 gcatgcaacg accttgcaag agtactgact caatgggaca taatacagca tggcgatctg
     1141 gcagggtata tttatcagaa tatcaaagat acagatgaca aaaaaaagaa agggcaatac
     1201 tttacgccac ctgctattgt acagtacatg gttgaacatg cgctgcaata ccacgacaat
     1261 ccttttacag ttaaaattct ggatcctgcc tgtggatctg gtcagttttt gatttatata
     1321 tatcaatacc tactggaact ctacaaacag catggcattt ctcacgagga tgctgcatac
     1381 accatctgtc aaaataatct atttggctat gatattgatc ctgttgcctg catgattgca
     1441 cagtggaata tacaaaaact ttctgggacc cataccaaca ttttccacag agacatgctt
     1501 tcaacagacg atctttttaa taaacataat tccttagtat ttgatatcat tattggcaac
     1561 cctccctggg gcgcacattt tacacatgaa caaaagcaat atttcaaaca gcattatttt
     1621 tcagcaataa gtggtataaa cagttttacg ttatttattg aacggtcact ttcacttttg
     1681 catgaacgcg gtattttgtc atttttaatc cctgaagcat acctcaatat taaagcacat
     1741 cagaactcac ggcaaatagt actttcaaat actgtgatta aagaaatagt acaatgggga
     1801 gattgtttta agggagtgta tgcacctgcc atatcgctta ctgctcaaaa ggaggaacgg
     1861 gaagaaattc gaaagaaaca tattgttcac ataaagcatg caaaatcatt aacaactatc
     1921 gcccacatga taccccaaca aatgtattgt actacccatg aatctatttt tcccatcaat
     1981 tatacccgta aggcggttgc actattagaa cacattcata cactatcata tattacatta
     2041 caagacagag taaaattttt tcttggcatt gtcactggca acaacgacta ccatataagc
     2101 acaatcaaag actcaagaca tcctgaccca ataatcatag gtaaagatgt agaaaaattt
     2161 cgtattaatt tcagcaatca ctattttaaa tacaatgtat ccacattaca acaggttgct
     2221 gaacagcaat gttaccttac aagaaataaa attctatata aatttattgg cagacacctt
     2281 acctttgccg ttgacccgca tggatcatat acacttaaca atgttaatgg ttttattcca
     2341 ctgtcaccac agctggatcc cgatgttctt gtagctattt taaattctcg cctactccaa
     2401 tatatttatg aaactcaatt ttttactgtt aaagtcttac gtggtaatct ggagaaacta
     2461 ccaataaaaa tcattaattc tacaaacaaa aagcttatga aaaaattatc tttagaactc
     2521 agggaagaag ctgacagagt taaacgaaaa atattaatgg atacaattga tgatattgtt
     2581 tttcatgaat ataggattaa tgatatgctt gcacaccata tccatgaaag cattaccaca
     2641 ggtatttcaa tagcataata attattaatt tgaacaactc ttaaaaatta ttaccccatc
     2701 tacagtatca tcgctctttt catcaataaa cgcaattccc ttcgatggat acatatatat
     2761 tgatccattt tcatctttga gaactttcat tccttcattg ccataattaa atactattgc
     2821 cattacacct tttgataccc g
//
LOCUS       DAOSZP010000278         2840 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_27507_length_2840_cov_3.075045, whole genome shotgun sequence.
ACCESSION   DAOSZP010000278 DAOSZP010000000
VERSION     DAOSZP010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2840
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27507_length_2840_cov_3.075045"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..107
                     /locus_tag="PK348_06755"
     CDS             <1..107
                     /locus_tag="PK348_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011887843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65949.1"
                     /translation="RKLLEKQKEGKKRLKQIGSVEIPQEAFLTILKID"
     gene            complement(152..2704)
                     /gene="glgP"
                     /locus_tag="PK348_06760"
     CDS             complement(152..2704)
                     /gene="glgP"
                     /locus_tag="PK348_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014932951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HXK65950.1"
                     /translation="MSTIQTFRVYPYLPDNLKPLKTIAYNMWWVWNFEAIELFRRLNV
                     ELWRESNHNPVALLGMLSQSELDEAADSESFMAHLKRVSEELEWHLTKKSWFEEAYPT
                     YKDMQIAYFSAEFGIHECLPIYSGGLGVLAGDHIKSASELGLPFFAVGLLYRLGYFHQ
                     YLNIDGWQQERYPETDFHNLPLQLVMKDDKPLIIDVEFPGRNVYAQVWECIIGRIRLY
                     LLDTNISENSIEDRTITDQLYGGDVEMRIKQEMLLGIGGYRMLKALGKNISVYHMNEG
                     HSAFLSIERIYDAMVTHKMSFNEALEFVTAGTVFTTHTPVPAGNDRFSPELIDKYLSH
                     YYQKLGISREEFLGLGRENPHDKLETFCMTVLAIKTAAHCNGVSKLHGAVSRKMWRNI
                     WPGLPVNEVPIHHVTNGVQILSWTSDEMMRLLNRYLGPRWIDNPVDKTIWKNIDTIPD
                     SELWRCRERLRERVVAFARKKLREQLIARGAPRAEVEKADSVLDSEALTIGFARRFAT
                     YKRATLILKDMQRLERILLDKERPVQIIFAGKAHPRDNMGKELIKRVINLGRDDRFRS
                     KIVFLEDYDINIARYLVQGVDVWLNTPIRPQEASGTSGMKVVPNGALNISVLDGWWPE
                     AFNGNNGWAIGKGEEYQDPEYQDEVESLSLYNILEQEVIPQFYARGADGIPREWVRKI
                     KESMKTILPQFNTNRMVKEYTEMYYLTAYDSYVKFSKDNFKASRELEKWKLNIRQKWQ
                     QVTVDSISHESKKEIIVGSQLKAKAVINLGELKPADVQVELYFGNLNQKGDIEEGAAL
                     PMLMVEDLGNGKYTYEGRMLCLKSGQFGFTVRVIPLHADMPRKYESRMITWA"
BASE COUNT          843 a          648 c          480 g          869 t
ORIGIN      
        1 aacgtaagct actggaaaag cagaaggaag ggaaaaagcg cctgaagcag attggttctg
       61 tggaaattcc acaggaagca ttccttacaa ttttaaagat cgattaaaaa ctacataatc
      121 ccactgctaa tggcagcagg attaaacagt attatgccca ggtaatcatc cgcgattcat
      181 atttacgtgg catgtcagca tgtaatggga taaccctcac agtgaatcca aactgacctg
      241 atttaaggca aagcatgcgc ccttcgtatg tatatttacc atttcccaga tcttcaacca
      301 tgagcatagg caatgcagca ccttcttcga tatcaccttt ttgatttaaa tttccaaaat
      361 ataattcaac ctgcacatca gctggtttta attcacctaa gttgataaca gcttttgctt
      421 tcaattgaga accaactatt atttcttttt tgctttcgtg agaaatagaa tcaacagtaa
      481 cctgctgcca tttctgccgt atattaagtt tccacttttc aagttcacgt gaagctttga
      541 agttatcttt actgaacttg acgtaactat cataggcagt aaggtaatac atttcagtat
      601 attctttcac catcctgtta gtattaaact ggggtaaaat tgtcttcatg gattctttaa
      661 tttttcgaac ccactcacgt ggtattccat ctgcacctct tgcataaaat tgtggaataa
      721 cttcctgttc aagtatgttg tacaatgaca ggctttctac ttcatcctga tattcgggat
      781 cctgatattc ctcacccttg ccaattgccc agccattatt cccattgaat gcttctggcc
      841 accaaccatc aagtacgctt atattgagtg caccattggg gacaaccttc atgccgctgg
      901 tacctgatgc ttcctgagga cgtataggtg tgtttaacca cacatccact ccctgaacaa
      961 gataccgtgc aatgttaata tcataatctt ccaggaacac tattttgctt ctgaatcggt
     1021 catctcggcc taaattgata acccgtttaa tcaattcctt gcccatatta tcgcgcggat
     1081 gagctttccc ggcaaagata atctgaacag gcctttcttt atcaaggagt atgcgttcca
     1141 gtcgttgcat atcttttaat attaaagttg ctctcttgta tgttgcaaaa cgccgtgcaa
     1201 aacctatggt aagagcttcc gaatcaagaa ctgaatctgc tttttcaact tcagcgcgag
     1261 gagcaccacg ggcaataagt tgttcacgca atttttttcg tgcaaatgca actaccctct
     1321 cccgtaagcg ctcacggcaa cgccataatt cagaatcagg gattgtatca atatttttcc
     1381 atatagtttt atcaacggga ttatcaatcc accgcggtcc taaataacgg ttgagcaaac
     1441 gcatcatttc atctgatgtc catgacaata tctgcacgcc attagttaca tgatggatgg
     1501 ggacttcgtt cacgggtaat cccggccaga tattcctcca catttttcgt gaaactgctc
     1561 catgtaactt tgatacacca ttacaatgtg cagcagtttt tattgccagc accgtcatac
     1621 agaatgtttc taatttatca tgcgggtttt cccttcccaa acctaaaaac tcttcacgcg
     1681 atatacccaa cttctggtaa tagtgagaca aatacttatc aatcaactca ggcgaaaatc
     1741 tgtcattgcc tgcaggtacg ggtgtatgtg tagtaaaaac agtaccagca gtaacgaact
     1801 ccagcgcttc gttaaaactc attttgtggg taaccattgc atcatagatg cgttctatac
     1861 tcaggaatgc cgaatgccct tcattcatat ggtacactga tatattctta cccagagctt
     1921 tgagcattct atacccgcca atcccaagaa gcatttcctg ttttatccgc atttcaacat
     1981 caccaccata gagttgatca gtaatggtac gatcctcaat actgttttca gaaatattgg
     2041 tgtcaagcag gtacaatcgt atgcgcccaa tgatgcattc ccacacctga gcatatacat
     2101 ttctaccagg gaattcaaca tcaatgatta atggtttatc atccttcata accagctgca
     2161 aaggaaggtt atgaaaatca gtttctggat aacgttcctg ctgccagcca tcaatattta
     2221 aatactgatg gaaatatccc aacctgtata aaaggccaac tgcaaaaaat ggcaacccca
     2281 attcacttgc tgactttata tgatctcctg ccagaacacc cagaccacca gaataaatgg
     2341 gcaggcattc atgaatacca aactctgcac tgaaataggc aatctgcatg tctttatatg
     2401 ttggatacgc ttcttcaaac catgattttt ttgtcaggtg ccattccaat tcttctgaaa
     2461 cacgctttaa atgagccata aaactttctg aatcagccgc ttcatctaat tcagattgtg
     2521 aaagcatacc taatagagca acgggattat gatttgactc gcgccacaat tctacattaa
     2581 ggcggcggaa taattcaatt gcttcaaagt tccataccca ccacatatta tatgcaattg
     2641 ttttcaatgg cttaaggtta tcaggcaaat atggataaac tctgaacgtc tgtattgtgc
     2701 tcatatcccc tcacattctg aaaagttttt tcttatagta cagtgcatca tatcgcaagt
     2761 caagatttta atcattataa ttacaagatt tttgccatga actatcgcat aaatttttta
     2821 aataaaaaaa taatgatata
//
LOCUS       DAOSZP010000279         2834 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_27590_length_2834_cov_2.772220, whole genome shotgun sequence.
ACCESSION   DAOSZP010000279 DAOSZP010000000
VERSION     DAOSZP010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2834
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27590_length_2834_cov_2.772220"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(6..830)
                     /gene="rplB"
                     /locus_tag="PK348_06765"
     CDS             complement(6..830)
                     /gene="rplB"
                     /locus_tag="PK348_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HXK65951.1"
                     /translation="MGLKKYRPLTPTLRYQTVLDFSEITTDEPLKSLTKGKKSYAGRG
                     FKGQVSVRRKGGGHKRRYRIIDFKRNKYNIEGKVASLEYDPNRSANIALIVYKDGEKR
                     YIIAPDSVKVGDTIVSGENVEVKPGNAMPLKNIPDGTNIYNIELHKGKGGQLVRSAGT
                     SAVITAKEGDYCLIKLPSGEIRKIHCECYATIGEVCNKDHINVDIGKAGRSRWLGRRP
                     KVRGVAMNPVDHPLGGGEGKSSGGRHPCSPTGKPTKGYKTRKKKKYSDKMIIKRRK"
     gene            complement(864..1148)
                     /gene="rplW"
                     /locus_tag="PK348_06770"
     CDS             complement(864..1148)
                     /gene="rplW"
                     /locus_tag="PK348_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006235033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HXK65952.1"
                     /translation="MDVNDIIIRPVITEKATELAKQNKYVFRVHKKANKDMIEKAISS
                     IFGVTPVKVNVMIVRGKRKRVRYNYGYTASWKKAIVTLKEGDKIEVFESK"
     gene            complement(1151..1777)
                     /gene="rplD"
                     /locus_tag="PK348_06775"
     CDS             complement(1151..1777)
                     /gene="rplD"
                     /locus_tag="PK348_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014454653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HXK65953.1"
                     /translation="MVVDVYSVDGKVKGQIELNETIFNAKINDVLIYELIKAANANLR
                     QGTHDTRERSDVSGGGAKPWRQKGTGRARQGSIRAPQWKGGGVVFGPHPRDYRIELPK
                     GIRQEAYRSLLSLKFKEGAVKVVEDFSVESGKTKDMASIAKKLNVKKGVLITDSEDTM
                     LKRAIRNIPWFVYNNVKRLSSRDIFYTQTVLITESAVKIINSKYAKGE"
     gene            complement(1789..2406)
                     /gene="rplC"
                     /locus_tag="PK348_06780"
     CDS             complement(1789..2406)
                     /gene="rplC"
                     /locus_tag="PK348_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014553830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HXK65954.1"
                     /translation="MKKALIGKKIGMSQMFLDDGTVVPVTVCELGPCVVVQKKTVEKD
                     GYNALKLGYGETKAHRLTKPILEDLKKKNLPLLKVFREVENFDENLQEGSVITCEIFT
                     ENQMVDVTGISKGKGFAGVIKRHGFHGGRETHGSNFHRAPGSIGANTFPAEVWKGKKL
                     PGRHGGKTVTVKNLKIVKIIKDKNLVLISGAVPGTVNSIITVQSK"
     gene            complement(2491..2808)
                     /gene="rpsJ"
                     /locus_tag="PK348_06785"
     CDS             complement(2491..2808)
                     /gene="rpsJ"
                     /locus_tag="PK348_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008593919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HXK65955.1"
                     /translation="MAKLTGQRIRVKLRSFDPYLLDQSAAKIVATTNRSGAKVAGPIP
                     LPTRIEKFCVLRSPHVNKKSREQFEIRTHKRLIDIIDPNSDTVEALMKLELPAGVSVD
                     IKS"
BASE COUNT          758 a          684 c          413 g          979 t
ORIGIN      
        1 agcaattact tccttcgttt aataatcatt ttgtcactgt attttttctt cttcctggtc
       61 ttataccctt tggtaggttt gccagttggc gaacaggggt gccttcctcc ggacgattta
      121 ccttcgccac caccaagcgg atggtcaacc gggttcattg caacacctct tacctttggc
      181 cttctaccta accatctact tcttccagcc ttgccaatat caacgttaat atgatcttta
      241 ttacatactt caccaattgt tgcatagcat tcacaatgta tctttctgat ctcgccggag
      301 ggtaatttaa tcaggcaata atcaccttct tttgcggtta taacggcact cgtacctgct
      361 gaacgcacta actgtccacc tttaccttta tgcaactcaa tattataaat attggtccca
      421 tccggtatgt tttttaaagg cattgcattg cctggtttaa cctcgacatt ttcgccagaa
      481 actatcgtat caccaacctt taccgaatca ggtgcaataa tgtacctttt ttcaccgtct
      541 ttataaacga ttaaagcaat atttgcactt cggtttggat catattctaa tgaagcaacc
      601 ttgccttcta tattatattt attgcgcttg aaatctataa ttctatagcg gcgcttatgt
      661 ccaccacctt ttcttctgac agaaacctga cctttaaagc ctctacccgc ataacttttc
      721 ttgccctttg taagcgactt caacggttca tcagtggtaa tctcactgaa atccagtact
      781 gtctgatatc gtaatgttgg tgttaatggc cgatactttt tcaaacccat tgtcgttacc
      841 tttatcgcta tagtaaaaaa ttcttatttt gattcaaaaa cttcgatttt atcgccttct
      901 tttaacgtaa ctattgcttt tttccatgat gctgtatatc catagttata ccgcactctt
      961 ttacgtttgc cccgtacaat cataacattt acttttactg gtgtaacacc aaaaatactg
     1021 cttatagcct tctctatcat atctttattt gctttcttgt gtactctaaa cacgtatttg
     1081 ttttgttttg caagttcagt tgccttttct gtaataacag ggcgtatgat gatatcatta
     1141 acatccataa ttactcacct tttgcatact tcgaattaat aatttttaca gcgctctcgg
     1201 ttattaacac tgtctgagta tagaatatat ccctgcttga taaccgtttt acattattat
     1261 acacaaacca tggaatattt cgtattgctc tcttcagcat ggtatcttca ctatccgtga
     1321 tcagtacacc ttttttaaca ttaagctttt tggctattga agccatatcc ttagttttgc
     1381 cactttctac tgaaaagtcc tcaacaactt taactgctcc ttctttgaac ttcagggata
     1441 ataacgaacg atatgcttcc tgcctgatgc ctttaggtaa ttcaatgcga tagtcacgcg
     1501 gatgtggacc aaatacaacg ccacctccct tccactgtgg agcacggata gatccctggc
     1561 gagctctacc tgtacccttc tgcctccatg gctttgctcc accacccgaa acatcactac
     1621 gctcccgggt atcatgagtt ccctgacgca aattggcatt tgcagcctta atcaattcat
     1681 atatcagaac atcattaatc tttgcattaa agatggtttc attcaactca atctgtcctt
     1741 taacttttcc atcaactgaa taaacatcaa caaccataat aaccctgcct atttgctttg
     1801 tactgtaata attgaattaa cggtaccagg gacagcacca gatattaata ctaaattctt
     1861 atctttgatt atcttaacta tcttcaaatt tttaacggta acagtttttc caccatgcct
     1921 tcctggcaat tttttcccct tccaaacttc agcagggaat gtgtttgcac caatggagcc
     1981 aggagctctg tggaagtttg atccatgagt ttctctcccg ccatggaatc catgcctttt
     2041 aataactcca gcaaatccct ttcctttgga aataccggta acatcaacca tctggttttc
     2101 agtaaatatc tcacaggtaa taacacttcc ttcctgaaga ttttcatcaa aattttcaac
     2161 ttccctgaat acctttaaca aaggaagatt tttcttcttt aaatcctcta atataggttt
     2221 tgtaagacga tgagcttttg tttcaccgta tcccagcttc aatgcattat atccgtcctt
     2281 ttcaacggtc ttcttctgta caacaacgca gggaccaagt tcacatacag taaccggaac
     2341 caccgtccca tcgtctaaaa acatctgact catccctatc tttttgccaa ttaaagcctt
     2401 tttcattttc ctatcccatg catcgattat aagactgtca cactcttttt taaagagcta
     2461 tctaacaaat ttccatgaaa gatgaaataa ttatgatttt atatctacag aaacaccagc
     2521 aggcaattcc aacttcataa gcgcttcaac tgtatcggag ttaggatcga tgatatcgat
     2581 cagacgctta tgagtccgta tctcaaactg ctcacgcgac ttcttgttaa catgagggga
     2641 acgcaataca caaaattttt caattcgtgt aggtagcggt ataggacctg ctacttttgc
     2701 accactcctg ttagttgtcg caactatttt agctgccgat tgatctaata aatatggatc
     2761 aaatgatctc aatttcactc taattcgttg tcccgtaagc tttgccactg taacaccttt
     2821 ctttcatcca aata
//
LOCUS       DAOSZP010000280         2834 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_27591_length_2834_cov_2.519252, whole genome shotgun sequence.
ACCESSION   DAOSZP010000280 DAOSZP010000000
VERSION     DAOSZP010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2834
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27591_length_2834_cov_2.519252"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..548)
                     /locus_tag="PK348_06790"
     CDS             complement(<1..548)
                     /locus_tag="PK348_06790"
                     /inference="COORDINATES: protein motif:HMM:NF024601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65956.1"
                     /translation="MDNSFINSALRKIWKQSRFTSYFYHAVEFVHTQSIPTLSLVASQ
                     RMVLYYNPEFLNTISQDEFIGLLVHEMFHVILEHDHRAKTGDIVLQNIAQDMVVNTFI
                     SNHSKTFFSHKGQYQWDVPVLTIPQGLPFIPQEFYDETAIYDPVWEDVYRWLLQKQNE
                     LKVIQHHGDIEKQKGMFHDDRYA"
     gene            complement(554..1711)
                     /locus_tag="PK348_06795"
     CDS             complement(554..1711)
                     /locus_tag="PK348_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015948335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65957.1"
                     /translation="MKRPVEVSTIELIEILDRYLQTEGAINYAIKIVGYPGVGKSAIV
                     EQVAKKHNYYYIDTRLAFKENVDLGGYPVPDNNLKRMIYFRPRFIPPETVPEGYNGIL
                     WFLDEANRAHPTVIQTLFQIITERMCGEHILPPKTAIVLAGNLGDSDNTVITDFDDSA
                     LDGRLAIFNLIPDASNWLQWAIEEGIHPAVIQYISTYPDRLWDSSAVNPNPRGWHQVS
                     DTLYKAYNFQTEEQFRIYLLNNDYKVLQKIIISLIGDVAGTDFILQYVSPRAISSNDI
                     LAGDEKKLQQLIDNTIPTEDILWAVNGAIHILYTENVKRSGSPDEQLLKKLSHFLKFI
                     SFTRADTRVSFMYSLVRDCGLFTQIPDALNYIDNPEEKQSIMEKVQVFLDV"
     gene            complement(1757..>2834)
                     /locus_tag="PK348_06800"
     CDS             complement(1757..>2834)
                     /locus_tag="PK348_06800"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HXK65958.1"
                     /translation="VFAKTDKKDTVTGEPLQDIVVISQNDWLIIPHLYYKPETQFAYG
                     VVFLTYFQDIIMSIPPIKLRPTTLAITFTHTQNDQFIFQVFPEFYFFRDKYHIKNEFQ
                     YMNYPDKFYGIGQDTTENDREEYTSRIFSFKNIIERAFIPGFYIGLLYHIDVRKTTQA
                     QDGGLIDSNLLPGADKGLVSGLGFTINYDNRDDILFTRDGSYANFTITQYSKYIKSDF
                     DYINVTFDYRFFEPFWFSHVLAVQFISGYIHGQAPFYLLSQMGGAKLMRGLYEGRYRD
                     RNVIVVHAEYRFPLFWRFIGCGFAGAGEVAQTIREFNKEHIIYTWGGGLRFKLKKDQT
                     LNARLDIGFSKNFTGVYVTIGEAI"
BASE COUNT          910 a          555 c          460 g          909 t
ORIGIN      
        1 gcatacctat catcatgaaa cattcctttt tgcttttcaa tatcaccatg atgttgaata
       61 acctttaatt cattttgttt ttgtaatagc caccgatata catcttccca caccggatca
      121 tagatagcgg tttcatcata aaactcctgt ggaataaatg gtaaaccctg aggaatagtg
      181 agcacaggca catcccactg atattgcccc ttatgggaaa agaaagtttt gctatgattg
      241 ctaataaatg tatttaccac catatcctgg gcaatattct gcaatacaat atcacctgtc
      301 ttagccctgt ggtcatgttc cagtataaca tgaaacattt catgaacaag aaggccaata
      361 aattcatcct gcgaaatagt attcaaaaac tcaggattat aatacaaaac cattctctgt
      421 gatgcaacca gtgataacgt tgggattgat tgtgtgtgta caaattcaac ggcgtgataa
      481 aaatatgagg tgaatctgct ctgcttccag attttacgaa gtgcactatt aataaatgaa
      541 ttgtccattg ctattacaca tcaagaaaaa cctgaacttt ttccataata ctctgttttt
      601 cttccgggtt atcaatatag tttaatgcat ccggtatttg tgtaaacagc ccgcagtctc
      661 ttaccaggga atacataaat gacactcgtg tgtcagcacg tgtaaaagat ataaatttca
      721 gaaaatgaga taactttttt aataattgtt catcagggct accacttctt ttgacatttt
      781 cagtatacag aatatgaatt gcgccattaa cagcccacaa tatatcctct gtgggaatag
      841 tattatctat aagttgctgc aatttttttt catcacctgc aagaatatca ttgctgctta
      901 tggcacgcgg agatacatac tgtaaaataa aatctgtgcc ggcaacatcg ccaattaacg
      961 atataataat tttttgaagc actttataat cattattaag gagatagatc ctgaattgct
     1021 cctcagtctg gaaattgtac gccttataca gagtatctga aacctgatgc catccgcgtg
     1081 gatttggatt aacggccgaa ctatcccata gcctgtccgg gtatgttgat atgtactgga
     1141 taaccgcagg atgaatacct tcttcaattg cccattgcag ccagtttgaa gcatcaggga
     1201 ttagattaaa tattgccaat cttccatcaa gagctgaatc atcaaaatca gtaattacgg
     1261 tattatctga atcacctaaa ttacccgcaa ggacaattgc agtttttggt ggcaatatat
     1321 gttcgccaca catacgctca gttataatct ggaataatgt ctgaatgaca gttgggtgtg
     1381 ctctgtttgc ttcatctaaa aaccataaaa ttccattata cccttctggc acagtttcgg
     1441 gaggaataaa tcgtggcctg aaataaatca ttcttttaag attgttatcg ggtacaggat
     1501 agccacctaa gtccacattt tccttgaatg ctaaacgtgt atctatgtaa taataattat
     1561 gcttttttgc aacctgctct acaattgctg attttcctac accaggatac ccaactattt
     1621 taattgcata atttattgca ccttcagttt gcaggtatct gtccagaatc tcaattaatt
     1681 cgatagttga tacttctact ggccgtttca tgataattaa ttctatcgta aacactatat
     1741 ttattcaata atttatttat atagcctcac ctattgtcac ataaacaccg gtaaagtttt
     1801 tgctaaatcc tatatcaagg cgtgcattaa gagtctgatc tttctttaac ttaaatcgca
     1861 aaccgccacc ccatgtgtag ataatatgtt ctttattaaa ttcacgtatt gtctgggcaa
     1921 cctcacccgc accagcaaaa ccacacccaa taaacctcca gaataaagga aatctatatt
     1981 ctgcatgaac aacgattaca ttacggtccc tgtatcttcc ttcatacaaa ccgcgcataa
     2041 gctttgctcc tcccatttgt gacaataaat aaaaaggggc ctgaccatgt atataaccag
     2101 aaataaactg caccgccaga acatgagaaa accagaaagg ttcaaagaaa cgataatcaa
     2161 aggttacatt aatataatca aaatcacttt taatatattt tgaatactgt gtaatggtaa
     2221 aattggcata tgaaccatca cgtgtaaaca gaatgtcgtc acgattgtca taatttatgg
     2281 taaagccaag accggaaaca agtcctttat ctgctccagg taacaaattt gaatcaatga
     2341 gtccgccatc ttgagcctgt gttgtttttc ttacatcaat gtgatacaac aaaccaatat
     2401 agaatccagg aataaatgca cgttcaataa tatttttaaa ggaaaatatt ctggaggtat
     2461 attcttccct gtcattttct gtagtatcct ggccaatccc ataaaattta tcaggataat
     2521 tcatatactg gaattcattt ttgatatgat atttgtctct aaaaaaatag aactcaggaa
     2581 aaacttgaaa tatgaactga tcattttgtg tatgggtaaa tgttattgct aatgttgttg
     2641 gacgcaactt tattggcggt atgctcatta ttatatcctg aaagtatgtc agaaaaacaa
     2701 caccataggc aaactgtgtt tcaggtttat aataaagatg tggtattatc agccagtcat
     2761 tctggcttat gacaactata tcctgtaatg gttcacctgt tacagtatct tttttgtcag
     2821 tttttgcaaa aact
//
LOCUS       DAOSZP010000281         2831 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_27625_length_2831_cov_3.282421, whole genome shotgun sequence.
ACCESSION   DAOSZP010000281 DAOSZP010000000
VERSION     DAOSZP010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2831)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2831)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2831
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27625_length_2831_cov_3.282421"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..667)
                     /locus_tag="PK348_06805"
     CDS             complement(<1..667)
                     /locus_tag="PK348_06805"
                     /inference="COORDINATES: protein motif:HMM:NF040131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65959.1"
                     /translation="MNTIEFFLEPSKENYHTLRVKAEDGSFRYIHSKYYPTKESSYLQ
                     DIVNPEKYDACIILGIGLGYHLKYLATIIPFFKQIICIDINNYLTNANIPQDIQAIIH
                     HPSVQLVFVNPDNLQQLRDTIQLQFSKGIQVIDHPASFHAFPHIFSSIKKIIQQIVDK
                     SVSNHLTSSLFAYRYFKNALLNLNQLPECFAVTQCKGIHAHQPCIIVAPGPSLDETLY
                     HLKK"
     gene            747..1568
                     /locus_tag="PK348_06810"
     CDS             747..1568
                     /locus_tag="PK348_06810"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXK65960.1"
                     /translation="MIIDGHTHIFTNDMVQNKERYCNDRQFSLLYYGKARIAHAEMLV
                     ESMEHNGVDASLVLGFTWQKEEYAQMHNEYLATVQHTYKGKIYAFGAIALNGDVYKQI
                     ESIKKLGLYGIGEVAFYANGFNEEEEMLLGQILAIAEKFTLPVCLHVNEPVGHIYAGK
                     YYTDMRSLFALCSQYTNIPIICSHWGGGLLFYELMPEVKKSLTHVYYDTAATPFLYDS
                     GIYDIAIQMCGKEKILFGSDFPLLEYSRYKQHIEALGESAEYVLYKNVETVLGIA"
     gene            complement(1571..1834)
                     /locus_tag="PK348_06815"
     CDS             complement(1571..1834)
                     /locus_tag="PK348_06815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65961.1"
                     /translation="MFKHIAVIMIAIALVITGCKKTTITPKLFVTIQNEILDSDMQQT
                     TKEDIVKKYGITLQQYEEYEKQVESDPELKALVGKERLNIMKK"
     gene            1943..>2831
                     /gene="hisD"
                     /locus_tag="PK348_06820"
     CDS             1943..>2831
                     /gene="hisD"
                     /locus_tag="PK348_06820"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HXK65962.1"
                     /translation="MIAIRKLSDLTQKERNTLFQRFGDDFTGIMVNNVIPIVQKVRDE
                     GDKAVIEFTNKFDGVTLHEVCATEKEITAAYHNTDAKVIDSFTRAIENIREFHLHQKR
                     DSFIYTREDGTTLGMLYQPVESAAVYVPGGKASYPSSVLMGVIPAQIAGCKHISIITP
                     PSKDGAIASYIGAMAHILGITTIIKSGGAQGIAAAAFGTQTVPKADIIVGPGNIYVTA
                     AKAYLFSQGVIQIDSLAGPSEVLIIADEQANPHWVAWDLLSQAEHEEMAIAILVTTSE
                     SLAQKVITYIEEDINSGRGR"
BASE COUNT          885 a          452 c          627 g          867 t
ORIGIN      
        1 gttttttcag gtgatagagt gtttcatcca gagatgggcc aggggctacg atgatgcagg
       61 gctgatgggc atgaattcct ttacattgtg ttacagcaaa gcattcaggt aattggttca
      121 ggtttaataa tgcattttta aagtaccggt atgcaaacaa tgatgatgta agatgatttg
      181 atactgattt gtctactatc tgctgtatga ttttttttat ggatgaaaaa atgtgcggga
      241 aagcatgaaa ggaagcaggg tggtcaatga cctgtatacc tttggaaaat tgtaactgaa
      301 tagtatcacg taactgttga agattatcgg gattcacaaa taccaattgt accgaagggt
      361 gatgaataat tgcctgtata tcctgtggaa tattggcatt ggttagatag ttattaatat
      421 caatgcagat aatctgttta aaaaaaggta tgatagtggc caaatatttc agatggtatc
      481 caaggccaat gcctaaaata atacaggcat cgtatttttc agggttgaca atatcctgca
      541 ggtatgatga ttcttttgtt gggtagtatt tgctgtgaat atagcgaaaa ctaccatctt
      601 ccgccttaac acgaagtgta tggtagtttt ctttgctggg ttctaaaaaa aattctattg
      661 tattcataat aaatagtagt aaacgttgcc tgaagataaa atactaaaaa agtatatgat
      721 aatgtcaata cataataggg tatacgatga ttattgatgg gcatacacat atttttacta
      781 atgatatggt acaaaacaaa gaacgatatt gcaatgacag acaattttct ctgctatatt
      841 atggcaaagc acgcattgca catgctgaaa tgcttgtaga atcaatggaa cacaatggtg
      901 tggatgcctc gctggtgctg gggtttacct ggcaaaagga agagtatgct caaatgcaca
      961 atgagtatct tgctactgtg cagcatacat ataaagggaa aatatatgca tttggggcga
     1021 tagctctgaa cggtgatgtg tacaaacaga tagaatcaat taaaaagctg ggattatatg
     1081 gtattggtga agtagccttt tatgctaatg gatttaatga agaagaggaa atgttactgg
     1141 gacagattct tgctatagct gaaaagttta ctttgcctgt atgcctgcat gtcaatgaac
     1201 cggtgggcca tatctatgct ggaaaatatt atacagatat gcgcagtctt tttgcactgt
     1261 gttcgcagta tactaacata ccaattatct gttcacactg gggagggggc ctattatttt
     1321 atgagttgat gcctgaggtt aagaaatcct tgactcatgt gtattatgat actgcagcaa
     1381 ctccttttct gtatgattct ggtatttatg acatagccat acagatgtgt ggaaaagaaa
     1441 aaatactatt tgggagtgat ttcccactac tagagtattc acgctataaa caacatatag
     1501 aagcacttgg agaaagtgca gagtatgtct tatataagaa tgtggaaaca gttcttggaa
     1561 tagcgtgatg ctattttttc atgatgttga ggcgttcttt tccaaccagt gcttttaact
     1621 caggatcact ttcaacctgt ttttcatatt cttcatactg ttgaagagta atgccatatt
     1681 ttttaacgat gtcttctttt gttgtctgct gcatatcgct gtcaagaatt tcattttgta
     1741 tggtgacaaa aagttttggt gttatggtgg tttttttgca accggttata acaagagcta
     1801 tcgctatcat tattactgct atatgcttga acataggacc tccagaaata tttttttact
     1861 gtacagtttg tgatatttta aagaatggta atgataaagt caagaaataa tgaataacct
     1921 tctataataa tgaggagcaa ccatgatagc tatcaggaaa ttatctgatt tgacgcaaaa
     1981 agaacgcaat acactgtttc agcgttttgg tgatgatttt accggaataa tggttaataa
     2041 cgttattcca attgtgcaga aagtacgtga tgaaggtgac aaggctgtta tagaatttac
     2101 taataagttt gacggggtta cgttacacga agtgtgtgca acagagaaag aaattacagc
     2161 agcatatcat aatactgatg ccaaagtaat tgattcattt acaagggcaa tagaaaacat
     2221 acgcgaattc catctgcacc agaaaaggga ttcgttcatc tacacacggg aagatggtac
     2281 tactcttggt atgctgtatc aaccagtaga aagtgcagcg gtatatgtcc ctggtgggaa
     2341 agcgtcatat ccctcttcag tattgatggg agttattcca gcccagattg ctggatgcaa
     2401 acatatttca attataacac caccttcaaa ggacggggca atagcttcat atattggggc
     2461 aatggcacac atactgggta tcacaactat aatcaaatct ggcggtgcac agggtattgc
     2521 tgctgcagcg tttggaacac agactgtgcc caaagccgat attattgtgg gacccggcaa
     2581 tatctatgta actgctgcca aggcatatct cttcagccag ggagtcattc agattgattc
     2641 gcttgccgga ccaagtgagg tgttgataat agccgatgag caagcaaacc ctcactgggt
     2701 agcatgggat ttactgtcac aggcagagca tgaagagatg gccattgcaa ttcttgtgac
     2761 aacatctgaa tcgttggcac aaaaagtgat cacatatatt gaagaagata taaactccgg
     2821 aagaggcagg c
//
LOCUS       DAOSZP010000282         2826 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_27706_length_2826_cov_3.263443, whole genome shotgun sequence.
ACCESSION   DAOSZP010000282 DAOSZP010000000
VERSION     DAOSZP010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2826
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27706_length_2826_cov_3.263443"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..918
                     /locus_tag="PK348_06825"
     CDS             <1..918
                     /locus_tag="PK348_06825"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HXK65963.1"
                     /translation="TPSVDYTSMWDPANTILTLTPYKPLLFNTWYQIDIAKCEDLSHN
                     MMIQPVTCRFRTGSEYIRPVITGLYTTSISQNMAQTENFTIYEGANINDEIIITFNEP
                     IDTSSINRAFIISPQSTWDSYWGNLNQTCIIHFPQPLDVNTRYEIRINTNLKDVAQNT
                     LLNDLCVYIITNGNLSSIPSITNLECLIHEQKALTPLRVTDLGDESNENTTYEFTVHF
                     SAPILRNSVPDNVTIQCLYGEQPEISGNINQFRWIDDSQTLKLTIAAIEGGNVYKLTF
                     KGGTDGIKSSNGIPMQKDVWYIFYFHPVD"
     gene            946..2490
                     /locus_tag="PK348_06830"
     CDS             946..2490
                     /locus_tag="PK348_06830"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HXK65964.1"
                     /translation="MKVSLTIIFLSLTIVSCNYFVDIDPPEVISITPANRSVVLEDRP
                     VICVEFSESMDRPKTEEAFSFSGEHQPKGHFRWENNKLFFDLIENCKDATVYTIIIRS
                     SAEDSHGNNLKEDYTSSFSTSIDLIKPEAVSIQPADGARVSDIFSPLIVQFSEAVRLD
                     SVKKGFIITPYVVGDITLLNDNSTLVFTPYNNYIHGTTYTITLSTDIVDIAGNHLLKE
                     YKSIFICGTDFTLPTLNPESVEPPDYSVGCFAHYNGTEVRLQPFTTTEGIDKNADVLI
                     RFSKAMQRIDTNESINISPPCNTFFSWEDDHSLRIIPSSPLDLMQTYVLTISSQAKDI
                     AGNTLDKVYNFPFKIYSQYSQPIEVIPYGNNSKNIYQISWDSNGNADDSLSRLLENDD
                     IIESAPPYSCYKLINNTDVTVLIFRIQFTNTTNTLSTIASLDLPSAMQAISFSLIASQ
                     SSSTLIPKIWKIEVPTNHPDCVDIYMFNLTPQSYYRLSINHGINGIRDSVGNYMLQPF
                     TMYLTM"
     gene            2499..>2826
                     /locus_tag="PK348_06835"
     CDS             2499..>2826
                     /locus_tag="PK348_06835"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HXK65965.1"
                     /translation="MGTLIVQRPVTIIMSFSALCLFGIISACNLTMDLLPPVQYPEIS
                     IITLYPGASPSEIEQLITKPVEEAVNSVAGVTRIHSESIEGASLVVATLQWGSNVDFA
                     LLKVREK"
BASE COUNT          951 a          558 c          456 g          861 t
ORIGIN      
        1 acaccttcgg ttgattatac aagcatgtgg gatcctgcaa acaccatact aactctaact
       61 ccttacaaac cgttactgtt taatacctgg tatcaaattg atatagcaaa atgcgaggat
      121 ctttcccata acatgatgat acaacctgta acatgcaggt tcagaacagg tagtgaatat
      181 atacgtccag ttattacagg actctacaca acttcaattt cacaaaatat ggcgcaaact
      241 gaaaacttta caatatatga aggagcaaat ataaacgatg aaattattat tacttttaat
      301 gaaccaatag atacttcatc gataaataga gcatttatta tttcaccaca gtccacctgg
      361 gattcatact ggggaaattt aaatcaaacc tgtattattc atttcccgca accattagat
      421 gttaacacca gatatgaaat acgtattaat acaaatctaa aagatgtagc tcaaaacaca
      481 ttgctcaatg atctatgcgt atatattata acaaatggta atctttccag cataccatca
      541 attactaacc ttgaatgctt gatccatgaa caaaaagctc taactccttt acgtgttact
      601 gacttaggtg atgaatcgaa tgaaaacacc acctatgagt ttacagtaca tttttcagca
      661 cctattctca ggaattctgt accagataat gtaacaatcc aatgcctgta tggtgaacaa
      721 cctgaaataa gtggcaatat taatcaattc aggtggatag atgatagcca gacattgaaa
      781 ttaactatcg ctgcaattga aggcggaaat gtatacaaat taacatttaa aggtggcact
      841 gacggcataa agtcaagcaa tggcatacct atgcagaaag atgtatggta tatattttat
      901 tttcatccag tggattaaga aatgtttcat gtataggagt ttactatgaa agtatctctt
      961 accataattt tcctttctct tacaattgta tcctgtaatt attttgtgga tattgacccc
     1021 ccagaagtca ttagcataac ccctgctaac aggtccgtag ttttagaaga tagacctgtt
     1081 atttgcgtag aattttctga atcaatggat agaccaaaaa ccgaagaagc tttttctttt
     1141 agcggagaac atcagccaaa ggggcatttc cgatgggaaa acaataaact tttttttgac
     1201 cttattgaaa actgtaaaga cgcaactgta tatactatta ttattcgctc cagtgcggaa
     1261 gatagtcatg gcaacaacct taaagaagat tacacatcgt cattttcaac aagcatagac
     1321 ctcatcaaac ctgaagctgt atcaatacaa cctgcggatg gagcacgagt atctgacata
     1381 ttttctccac tgattgtaca attttctgaa gcagtcaggt tagattcagt aaaaaaaggc
     1441 tttataatta ccccatacgt tgttggtgat attacgctgc tgaatgataa ttctactcta
     1501 gtatttacac cgtataataa ctatattcat ggcacaacat acaccattac tctatcaaca
     1561 gatattgtag atatagcagg aaatcattta ttaaaagaat ataaaagtat ctttatatgt
     1621 ggtactgact ttacccttcc aactctaaat cctgaatcag tggagccacc tgattattct
     1681 gttggctgtt ttgctcatta taacggcaca gaagttcgac tgcagccttt cacaacaaca
     1741 gaaggcatcg ataaaaatgc agatgtactt atacgatttt caaaggctat gcagcgtatt
     1801 gatactaatg aaagtatcaa catatcccct ccctgtaata cctttttttc atgggaagat
     1861 gatcacagtc ttcgtataat accctcttca ccattagatt tgatgcaaac gtatgttctg
     1921 actatttctt cacaggccaa agacatagca ggcaatactc ttgataaggt atacaatttc
     1981 ccctttaaaa tatattcaca atattcacaa ccaattgaag ttattccata tggtaataat
     2041 agcaaaaata tatatcagat aagttgggat tccaatggga atgctgatga tagtttgtca
     2101 cgtctattag aaaatgatga tatcatcgaa tcggcaccac catattcatg ttacaaactc
     2161 ataaacaata ctgatgttac agtcttgata ttcaggatcc aatttaccaa tacaacaaat
     2221 acactgtcca ctattgcttc actggattta ccctcagcaa tgcaggctat atcatttagc
     2281 ctgattgcat cacaatcaag ttcaacactt atcccaaaaa tatggaagat agaagtacca
     2341 actaatcatc ccgattgtgt tgatatatat atgtttaatc ttactccaca atcgtattac
     2401 agattatcca ttaatcatgg catcaatgga ataagggata gcgtgggcaa ttacatgcta
     2461 caaccgttta ccatgtatct tactatgtaa tgcactatat gggaacatta atagtacaac
     2521 gccctgtaac aattatcatg tcatttagcg cactgtgcct ttttggcatt atatcagcgt
     2581 gtaacctcac catggatctg ctgcctcctg tacaatatcc tgagatttcc attattacac
     2641 tatatcctgg tgcttcccca tcggaaattg aacagcttat tacaaaacca gttgaagaag
     2701 cagttaacag cgtcgctggt gtaacgcgta tacattctga atcaattgaa ggcgcaagcc
     2761 tggtcgttgc cacattacaa tggggaagca atgttgattt tgctctctta aaggttcgcg
     2821 aaaaag
//
LOCUS       DAOSZP010000283         2823 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_27745_length_2823_cov_2.924494, whole genome shotgun sequence.
ACCESSION   DAOSZP010000283 DAOSZP010000000
VERSION     DAOSZP010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2823
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27745_length_2823_cov_2.924494"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1499
                     /locus_tag="PK348_06840"
     CDS             <1..1499
                     /locus_tag="PK348_06840"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HXK65966.1"
                     /translation="QAIQHIHFPATMDEAHHARVRLAFNELFFLQYYLHLSRHYIRET
                     HKHAKPHINDSLYSDFIQTLPFKLTDDQIKAIQEIINDLQADFPMNRLLQGDVGSGKT
                     IVSMIASLAVISRGQQVAFMAPTEILAQQHYTTFKKFLPDSVTVQLLTGSTPAAVRKE
                     ILAGLNNGSINIIIGTHALIQEHVQFANLGFIIIDEQHRFGVNQRASLRTKGYMPDLL
                     VMTATPIPRSLSLTLYGDLDISIIKQKPANRIPVTTLAFPSSKLEAVYRSLEKYMNEG
                     RQIYYVLPLIEDSEKLDLKSATATYTQLVKRFPTKTIALLHGKLPNEEKEKIMQQFNN
                     HTIDLLVSTTVIEVGIDVANASIIVIEHAERFGLSQLHQLRGRVGRGEYKSFCVLIHP
                     DDIPQESMQRIHILTTTDDGFVIAEEDLKMRGAGEFIGTRQHGQGIAFEFADPVNDFE
                     LISIAREEAQKQVERLKDIPALFENIKRTQAMNILEGLRKKHILALLS"
     gene            complement(1505..2206)
                     /locus_tag="PK348_06845"
     CDS             complement(1505..2206)
                     /locus_tag="PK348_06845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HXK65967.1"
                     /translation="MFTVKQYKGDENLKKILDKNNIPVDIYNLKAAILGVQASAGSFT
                     LRSLIKDFFSEEVLENKDVADQIESLWFYLLRLQRLQDGKLEQPDFDMKTREAYTAYV
                     NASIMRAETFLKYLSTGQLDEMLKNEKVNKIYSIFIGNVNLLKALIISMEEKWSQEDF
                     ASDTTFLKKLTGFVRVMWDTQIALKEHAKIFNLQKMNNKNIIEAIQQQTGTEIGRNDP
                     CPCGSGKKYKNCCGK"
     gene            complement(2221..>2823)
                     /locus_tag="PK348_06850"
     CDS             complement(2221..>2823)
                     /locus_tag="PK348_06850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65968.1"
                     /translation="TKTQLTDDQMKSELRRALLKLSIDKEKYDIVEDFIEFISKKNVS
                     ILHESVEYFDIPENDQWLFYYHLGPHTIFRLLLADIQISGEGYCFSSRDGKKIKKLIP
                     YEFLKTIVLEWCDNNVNNRDLPFDSVNALDLIRRQVSKKYFSEIDEFVAKIDAYLGTL
                     NPETLKKLDRKSFIKQKALQVYNQKQILIFNRFVDRTIFK"
BASE COUNT          892 a          571 c          471 g          889 t
ORIGIN      
        1 cacaggctat tcagcacatt cacttccctg ccacaatgga tgaagcacat catgcacgtg
       61 tgcgattggc cttcaatgaa ctttttttcc tacaatatta tttacatctt tcacggcact
      121 atatcagaga aacacataaa catgcaaaac ctcatataaa cgattcgctg tatagcgatt
      181 ttatccaaac cctgccgttc aaacttactg atgaccagat aaaagctata caggaaatta
      241 ttaatgattt acaagcagat tttcccatga accgtttgtt gcagggcgat gttggttctg
      301 gcaaaacgat agtgtctatg attgcaagcc ttgctgttat aagccgtgga cagcaggttg
      361 ccttcatggc accaacggaa atactggcac aacagcatta tactacattc aaaaagtttt
      421 taccagattc tgtaactgta cagcttctta ccggttcaac acctgctgcc gtacgcaaag
      481 aaattcttgc aggcttaaac aatggatcta tcaatatcat cattggcacc catgctctga
      541 ttcaggaaca cgtgcaattt gcaaatctgg gatttattat tattgatgaa caacatcgct
      601 ttggtgttaa tcagcgcgca agtctcagaa ccaaaggcta tatgccagat ctgttagtca
      661 tgactgcaac gcccattcca cggtcactat cgctaacatt gtatggtgat ctagacattt
      721 caataataaa acaaaagcct gcaaacagaa ttcctgttac aacacttgcc tttccatcat
      781 caaaacttga agctgtgtat cgctcactgg aaaaatatat gaatgaaggc agacagatat
      841 attacgtact gccactaatt gaagattcag aaaaactgga tttaaaatcc gcaacagcaa
      901 cctatacaca gcttgtgaag cgattcccaa caaaaacgat tgccctgtta catgggaagc
      961 tgcccaatga agaaaaagaa aaaatcatgc aacaatttaa taatcacacc attgaccttc
     1021 tggtcagcac aactgttatt gaggttggaa ttgatgttgc caatgcatca attattgtta
     1081 tagaacatgc tgaacggttt gggctttcac agcttcatca gttacgtggt cgtgttgggc
     1141 gtggtgaata caaatcgttt tgcgttttaa ttcatcctga tgatatacct caggaaagta
     1201 tgcaacgcat acatatttta acaacaaccg atgatggctt tgttatagca gaagaagatt
     1261 tgaaaatgcg tggagcaggt gaattcattg gcacccgtca gcacggacag ggtatagcgt
     1321 ttgaatttgc agatcccgtt aacgattttg aacttatcag tatagcccgg gaagaagcac
     1381 aaaagcaggt agaaagatta aaagatattc ctgcgctttt tgaaaatatt aaacgaacac
     1441 aggcaatgaa tatactggaa ggattaagaa aaaaacatat actggcactg ctatcgtaat
     1501 cagcttattt accgcaacag tttttatatt tcttgccact gccacatggg caaggatcat
     1561 tacgaccaat ttcagttccg gtttgttgct gaattgcttc aatgatattc ttattattca
     1621 ttttctgtaa attaaagatt ttagcgtgtt cctttaatgc aatttgagta tcccacatta
     1681 cccgtacaaa gcctgttaat tttttcagga aagtagtatc actggcaaaa tcctcctgtg
     1741 accacttttc ttccatgcta ataattaaag ctttcaggag attaacattg ccaataaata
     1801 ttgaatatat tttattcact ttttcgtttt tcagcatttc atctaattgg cctgtggata
     1861 aatattttaa aaacgtttca gcacgcatga tggaagcatt cacatacgct gtatatgctt
     1921 cgcgggtttt catgtcaaaa tccggctgct caagttttcc gtcctgcaag cgctgtagcc
     1981 gtaacaggta aaaccacaat gactcaatct gatctgccac atctttattt tccagcactt
     2041 cttcactaaa aaaatctttt ataagtgacc gcaacgtaaa actgcctgct gaagcctgta
     2101 ctcctaaaat agcagcttta agattatata tgtctacagg tatattattc ttatccagaa
     2161 tctttttaag attttcatca cctttatatt gtttaactgt gaacatagta atccttcacc
     2221 ctacttgaat attgttctat caacaaagcg gttaaaaatt aatatttgtt tctgattata
     2281 aacctgtaag gccttttgtt ttataaacga tttgcggtcc agctttttta atgtttcggg
     2341 gttcaatgtt ccaagatatg catcaatttt tgcaacaaac tcgtctatct cagaaaaata
     2401 tttttttgaa acctgcctgc gaataagatc aagagcatta acactgtcaa atggcaaatc
     2461 tctgttattg acgttattat cacaccattc taaaacaatt gtctttaaaa attcatatgg
     2521 aatcaacttc ttgattttct tgccatccct tgaactgaaa cagtaccctt cacctgatat
     2581 ctggatatcg gctaaaagca aacgaaatat ggtatgtggc cctaaatgat aataaaacaa
     2641 ccattgatca ttttcaggaa tatcaaaata ttcaacactt tcatgcaaga tagatacatt
     2701 ttttttactg ataaactcaa taaaatcttc aacaatatca tacttttctt tatcgataga
     2761 tagttttaat aaagccctcc gtaattctga tttcatctgg tcatcagtca attgtgtttt
     2821 ggt
//
LOCUS       DAOSZP010000284         2823 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_27746_length_2823_cov_2.924494, whole genome shotgun sequence.
ACCESSION   DAOSZP010000284 DAOSZP010000000
VERSION     DAOSZP010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2823
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27746_length_2823_cov_2.924494"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..607
                     /locus_tag="PK348_06855"
     CDS             <1..607
                     /locus_tag="PK348_06855"
                     /inference="COORDINATES: protein motif:HMM:NF018076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HXK65969.1"
                     /translation="SFNGIAVTPSVFIYPATKGKMCDSSYKYTIAINPKDAFGDGTHP
                     TTRMCIQTLESITGTIAPEKRSSYSLCDIGTGTGIIAIAAELFGFGTIDAFDIDPLAI
                     QRAQENAAYNGCNRINFFVSDIAKVRSETQYTIVVANLLSDIIEKNAAAIASLVEKGG
                     TLIASGIHDRWAQGIIALMQKYCTLVDKNIMDEWHCLVFKK"
     gene            626..1621
                     /locus_tag="PK348_06860"
     CDS             626..1621
                     /locus_tag="PK348_06860"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXK65970.1"
                     /translation="MDSCEEIYPGIFKIVQSKPRRIAPPVNIYIIAGDDGLIFDAGYG
                     DDTSIHLAGDAINSIQNRNKNWRILRVLPSHSHPDHFSGLKQLKSHYGLKVMLTEQMA
                     SVLSNKHAYRNSYKLSTLQKYRYSTVFERISELPLQVVFRTMYKKVYGIAFLQSHDTT
                     VRPGETIAINGYNFTIIHGPGHCNDHIMLYDNKEGILFAGDNILRSIYTWLGPPRSNL
                     QEYFDTLEACLRLPNLKLILSAHGSTIHNPKERIKEIIEYRKLRTRQVYSLVANETHG
                     ITFARLLNSIYPRSLERRWLAKGWIIVTLEYLVDNNILTFEGRKIKLGANDINTV"
     gene            complement(1670..2635)
                     /locus_tag="PK348_06865"
     CDS             complement(1670..2635)
                     /locus_tag="PK348_06865"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXK65971.1"
                     /translation="MFSVIRGMLFFTLIALTTGCSSLGMRNIPLDELKPKYANDASRF
                     ILINDTIIHIRDEGKKDGPVLLLIHGVCASLHTWDGWVEILKDHFRIIRFDIPGFGLS
                     GQIPSNNYTPEYSVDLLNKAIEHMGIKKLSIAGNSLGGFIAWKFAITHPDKVEKLILI
                     DSVGYPQEMPWLLKFSTNIFVRPFARIGMPRYFINKAVHEVYGDQSKVTDAIVDRYFE
                     LSQKEGNRSAWIDVFMVMKDYSKRKDLSKGLTDIQCPIMVMWGTKDIWIPYTTEFPKW
                     QKDLPHAVFKVYEGVGHIPMEEIPEQTAKDAFEFLTGKKLFTAKQ"
BASE COUNT          904 a          530 c          528 g          861 t
ORIGIN      
        1 ttcgttcaat ggtatagcag taaccccttc tgtatttatt tatcctgcaa caaagggaaa
       61 gatgtgcgat agttcataca aatacaccat tgccattaat ccaaaggatg cctttggtga
      121 tggaacccat cccaccacac gcatgtgtat tcaaacactt gagtccatta caggtactat
      181 cgccccagaa aaacgttcgt cgtattcact gtgtgatatt ggcaccggta ctggaattat
      241 tgccatagca gcagagcttt ttgggtttgg cactattgat gcatttgata ttgatccact
      301 ggcaatacag cgtgcacagg aaaatgcagc atacaatggg tgtaacagaa ttaatttttt
      361 tgtttctgat attgcaaaag tgagaagtga aacacagtat acaattgttg ttgcaaattt
      421 gctatcggat attattgaaa aaaatgctgc tgcaatagcg tctctggttg aaaaaggtgg
      481 aacgttaata gccagcggca tccatgatcg ctgggcacaa ggaattattg cattaatgca
      541 aaagtattgc acacttgttg ataaaaacat catggacgaa tggcattgcc tggtttttaa
      601 aaagtaaacg tgatggtgca tggtaatgga tagttgtgaa gaaatatatc ctggcatttt
      661 taaaatagtt cagtccaagc cacgcagaat tgctcctcct gtcaacatat atatcattgc
      721 cggcgatgat ggtttgatct ttgatgctgg ttatggggat gatacatcaa tacacctggc
      781 aggtgatgca attaactcta tacagaaccg taataaaaac tggcggattt tacgggtatt
      841 gccaagccac tcgcatcctg atcatttttc aggattaaag caattgaaga gtcattacgg
      901 gcttaaggtt atgcttactg agcaaatggc cagtgtttta tcaaataaac atgcatatag
      961 aaattcatat aaattgagca cattacaaaa ataccggtac tctacagtat ttgagcgtat
     1021 atcagaatta ccacttcagg ttgttttcag aaccatgtat aaaaaagtat atggtattgc
     1081 attcctgcaa tcgcatgaca ctacggtaag gccaggcgaa actattgcaa taaatgggta
     1141 taactttact atcatacatg gacccggtca ttgcaatgac catatcatgt tatatgataa
     1201 caaagaaggt atcctatttg caggtgacaa tatcctgcga agcatttata cctggcttgg
     1261 tccgcctcgt tccaacctgc aggaatattt tgatactctt gaagcgtgct tacgcctgcc
     1321 caatttaaaa ctaatattga gcgctcacgg cagcactatc cataatccaa aagaaagaat
     1381 aaaagaaatt atagaatacc gaaagctacg caccagacag gtgtactccc ttgttgcaaa
     1441 tgaaacacat ggcataacat ttgccagact tttaaatagt atatatcccc gctcattaga
     1501 gcggagatgg ttagcaaaag ggtggattat tgtcactctt gagtatctgg tggataacaa
     1561 tatactgaca tttgagggca gaaaaataaa gctgggggcc aatgatatta acacagtata
     1621 aaaaaattct catttagtaa taaaaactat cgcccctatc ccaatactat tattgtttag
     1681 ccgtgaaaag ctttttcccc gttaagaact caaaagcatc ttttgcagtt tgttcgggta
     1741 tctcttccat tggtatatgc ccaaccccct catacacttt aaaaactgca tgaggaaggt
     1801 ctttttgcca tttagggaac tcagtagtat agggtatcca gatatccttt gttccccaca
     1861 tcaccatgat aggacattga atatcagtaa ggccttttga aagatctttt cttttggaat
     1921 agtctttcat taccataaaa acatctatcc aggcacttct atttccttct ttttgcgata
     1981 gttcaaaata tctgtcaact attgcatcag tgactttgct ctgatctcca tatacttcat
     2041 ggacagcttt attgataaaa tatcgtggca tgcctattcg tgcaaatggt ctgacaaaaa
     2101 tatttgtgga aaatttcaat agccagggca tttcctgcgg atatccgaca gaatcaatta
     2161 agatgagttt ttcaacttta tcaggatgcg taatggcaaa cttccatgca ataaagcctc
     2221 ctaatgaatt tcctgcaatg gaaagtttct taatacccat atgttcaatt gctttgttta
     2281 gtaaatcgac actgtattca ggtgtataat tgttagatgg tatttgccct gataatccaa
     2341 aaccggggat atcaaatcgg ataattctga aatgatcctt caatatttct acccatccat
     2401 cccatgtatg taatgatgca cagaccccat gaatgagcaa taaaacagga ccatcttttt
     2461 tgccttcatc tcttatgtga atgattgtat cattaataag gataaagcgg gaagcatcat
     2521 ttgcatattt tggttttagt tcatcaagtg ggatatttcg catgccaagg gatgagcaac
     2581 ctgtagttaa agcaatcaat gtaaaaaata acattccacg tataacagaa aacatataat
     2641 acctccggaa aatacaatta tttatttatt gaatcagtta tgtaaaattt gtttaaaagt
     2701 gtatagcacg tacaggttta atgaatactg acaggtcggt ttatttgtca agaaagattc
     2761 actataaaca gtactataaa caaaaatttt gaaatcatca tggttgatga tggcagcccc
     2821 tta
//
LOCUS       DAOSZP010000285         2816 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_27837_length_2816_cov_2.718580, whole genome shotgun sequence.
ACCESSION   DAOSZP010000285 DAOSZP010000000
VERSION     DAOSZP010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2816
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27837_length_2816_cov_2.718580"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            158..463
                     /locus_tag="PK348_06870"
     CDS             158..463
                     /locus_tag="PK348_06870"
                     /inference="COORDINATES: protein motif:HMM:NF012675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HXK65972.1"
                     /translation="MIENITFTYRDGSHNAGNVTVYALSTCGHCKRALNFLDSNNVKY
                     RFVYVDLLPSEEKNKLKDYLVQKSNMYVAFPFLVIDDNTFFTGFIEEEWRRMLKLEK"
     gene            466..807
                     /locus_tag="PK348_06875"
     CDS             466..807
                     /locus_tag="PK348_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014454466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016730 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-thioredoxin reductase catalytic
                     domain-containing protein"
                     /protein_id="HXK65973.1"
                     /translation="MKKAKTLQDTLQFAQMVASKHGWVLHPDKEFLDILIEGLTINYN
                     RYGYYSCPCRDADGIRDQDKDIICPCDYCPPDIKEYGYCYCGLYNDPEFIKNGGMPKS
                     IPERRQYYFTK"
     regulatory      865..966
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            1045..2325
                     /gene="thiC"
                     /locus_tag="PK348_06880"
     CDS             1045..2325
                     /gene="thiC"
                     /locus_tag="PK348_06880"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016775544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HXK65974.1"
                     /translation="MTQLDFAKQGSITREMQQAAEYEGISAEELRNLIAKGWAVIPKN
                     IKRTFSVRAIGKGLLTKVNANIGTSTDHADIKEEMKKLEAAIYYGADSVMDLSTGGDL
                     HAIRKMVLEHSTVMVGAVPIYQVAAELSAKDKSILDMSENQLFDAIEQQCEEGIDYIT
                     VHCGVTQHSAELASKQRRVMGIVSRGGSLLAAWMRHHKKENPLYEHFDKLCAIAHKYD
                     VTLSLGDGLRPGAIADATDAAQIEELVILGQLVKKAREAGVQVIVEGPGHVPMDQITA
                     NIIAEKRICDNAPFYVLGPLTTDIAPGYDHIVGAIGGAIAALAGADFLCYVTPAEHLC
                     LPTVEDVRLGVIGTRIAAHSADIAKGIPKAIQQDFEMSQYRKALDWDGMFSKAIDPDM
                     ARARRKKSEDYEEKVCTMCGKLCAIKTHNECELL"
     gene            2340..>2816
                     /locus_tag="PK348_06885"
     CDS             2340..>2816
                     /locus_tag="PK348_06885"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015773145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HXK65975.1"
                     /translation="MVTGNDARIILENVRLQKPLIHNITNYVVMNYTANALLACGASP
                     VMAHAYNEVQEMTGIANALVLNIGTLENEWIHSMIIAAGTAHKKGIPVILDPVGAGAT
                     SLRTHTALKILNTGTISVIRGNASEVLALADNNAQTKGVDALHSVNDAIEVAMMIAD"
BASE COUNT          923 a          441 c          637 g          815 t
ORIGIN      
        1 ctttaacaga tatgtataaa acataatcga aataatatta attgacattg tttgattgag
       61 attgtatatt tttatacaat tatagtaata tacagaactg tttccatatg aagcaggaat
      121 aaaaaatgat gtgatacaat tctttaagga gatgcatatg atagaaaata taacttttac
      181 atacagggat ggctcacata atgcggggaa tgttaccgta tatgctcttt caacatgtgg
      241 tcattgtaaa cgtgcattga attttcttga tagcaataat gtcaaatacc gttttgtcta
      301 tgtggattta ttgccatcag aggaaaagaa taaattaaag gattatctgg ttcagaaatc
      361 aaatatgtat gttgcatttc cttttctggt tattgatgac aatacatttt tcacaggctt
      421 tattgaagaa gaatggcggc gtatgttaaa attggagaaa tagccatgaa aaaagctaaa
      481 acattacagg atacgttaca gtttgcacag atggtagcat ccaagcacgg gtgggtttta
      541 catccagata aagagtttct tgatatatta atagagggat taacaataaa ttataaccgt
      601 tatgggtatt actcctgtcc gtgcagggat gctgatggta ttcgtgatca agataaagat
      661 ataatatgcc cgtgtgatta ctgtccacct gacattaaag aatatggata ctgttattgt
      721 gggttatata atgatcctga atttattaaa aacgggggaa tgccaaaaag tatacccgaa
      781 cgaagacaat attattttac taaataattt ttctatttac ttgatttttt acataactgt
      841 tattatattg catttactaa aagttcttta aagcaggggt ggtttgtgca ggtgcacagg
      901 cctgagagta tacccttaga acctgatctg ggtaatgcca gcggagggag tagtttttat
      961 aattgcttct gttttcattt tatttttaaa aacttaaaaa accgcccaca cgctgggcgg
     1021 ttttttgtta tgagggttac cattatgaca cagttagatt ttgcaaaaca gggtagtatc
     1081 accagagaga tgcagcaagc agcggaatat gaagggatat cggctgaaga gttacgtaat
     1141 ttaatagcca aaggttgggc agtgatacca aaaaacatta aacgtacatt ttctgtgcgt
     1201 gccattggga aaggtttgct taccaaagta aatgcaaata taggaacatc aactgaccat
     1261 gctgatatta aagaagaaat gaaaaagcta gaagcagcta tttattatgg tgctgacagt
     1321 gtgatggacc tttcaacggg tggtgattta catgcaatta gaaaaatggt tctggagcat
     1381 tcaacagtaa tggtaggtgc tgtacctatt tatcaggtag ctgcagagct ttcggcaaag
     1441 gataaatcaa ttctggacat gtcagaaaac cagctttttg atgcaataga acagcaatgt
     1501 gaagaaggca ttgattatat tactgttcat tgcggtgtga cacaacatag tgctgagtta
     1561 gcatcaaagc aaagacgtgt tatgggaatt gttagccgtg gtgggtcact tttagcagca
     1621 tggatgagac atcataaaaa agaaaatcca ttgtatgagc attttgataa gttatgtgcg
     1681 atagctcata aatatgatgt gaccttaagt ttaggcgatg gattgcgtcc aggagccatt
     1741 gcagatgcta ctgatgctgc acagatagaa gaactggtaa tattaggaca gcttgtaaaa
     1801 aaagcacgtg aagctggtgt acaggtcatt gttgaaggcc caggccatgt tcccatggat
     1861 caaataactg caaacatcat tgcagaaaag cgtatctgtg acaatgcacc attttatgta
     1921 ctggggccat taacaactga tatcgctcca ggatatgacc atattgtagg ggctattggt
     1981 ggagctattg ccgcacttgc aggtgccgac tttttgtgct atgtgacgcc agctgagcat
     2041 ttatgcctgc caacagtgga agatgtacga ttgggtgtga taggcactcg cattgctgca
     2101 cactcggctg atattgctaa gggcattcca aaagccatac aacaggattt tgaaatgtca
     2161 caatacagaa aggcgctgga ttgggatggc atgttcagca aagcaattga tccagatatg
     2221 gctcgtgcac gcaggaaaaa aagtgaggat tatgaagaaa aggtgtgtac catgtgtggc
     2281 aagctgtgtg caatcaaaac acataatgag tgtgaattat tgtaaaaaag gagaaataaa
     2341 tggtaacagg taacgatgca cgtataattc ttgaaaatgt ccgattacaa aaaccgctga
     2401 tacataacat tactaattac gtggtaatga attacactgc aaatgcattg cttgcctgcg
     2461 gtgcttcacc ggtgatggca catgcatata atgaagtaca ggaaatgacc ggcattgcaa
     2521 atgcactggt acttaatatt gggacactgg aaaatgagtg gattcattca atgataattg
     2581 cggcaggtac tgcacataaa aaaggtatac cagtaatatt agaccctgtt ggtgctggtg
     2641 caacatcgtt acgaacgcac acagcactaa aaatattgaa cacaggtact atatcagtca
     2701 ttcgtgggaa tgcatcagaa gtgcttgcac tggctgacaa caatgcacag acaaaaggtg
     2761 ttgatgcctt acatagtgtt aatgatgcta ttgaggtggc aatgatgata gctgac
//
LOCUS       DAOSZP010000286         2814 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_27859_length_2814_cov_4.457775, whole genome shotgun sequence.
ACCESSION   DAOSZP010000286 DAOSZP010000000
VERSION     DAOSZP010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2814
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27859_length_2814_cov_4.457775"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="PK348_06890"
     CDS             complement(<1..362)
                     /locus_tag="PK348_06890"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HXK65976.1"
                     /translation="MERLWEKNYDFWYHSVNFQRMTVQDMLHCSAAVAPDKVATDFFG
                     TQITYYQLRLLSIKFAQALVKIGVKKGGRVCLQLPNSPQFAIAYFGTLMAGGIATLMN
                     PLYTADEVNQLISITQPNA"
     gene            complement(378..1283)
                     /locus_tag="PK348_06895"
     CDS             complement(378..1283)
                     /locus_tag="PK348_06895"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HXK65977.1"
                     /translation="MKRKTMQIHPSIYIHNADITPRGKKALIVEGGGMRGVFSAGVLF
                     AMGLRGLTHFDLYIGVSAGACNLASFLGRQYERNFYIMENWSASWRFINPFRIFWGNV
                     LDLDWLWDITISNYRLNLHEIFKDSSREFLVVVTSMESGKALYIKPNKENLEFVLKAS
                     SALPVLYRNKLFIEGQRVADGGVGDSIPVKEAYKRGARDIVVIRSQAKEYRKDKSESG
                     TLLKLLFRSYPEFVKALVQRPVNYNKAIEFIENPPDDTRITEICPPAWFKTKRTTTSK
                     QQLLKDYYSGIQTGFLLCNYYKSLQ"
     gene            complement(1273..>2814)
                     /locus_tag="PK348_06900"
     CDS             complement(1273..>2814)
                     /locus_tag="PK348_06900"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXK65978.1"
                     /translation="SCGGMGMPEMMYRSALEYFLAASISFTIYITLSNGATNLVRIFG
                     NEEQKQLYLHNMVTGRWGGTMCLTEPQAGSDVGAIKTKAYRQPDGTYKIKGQKIFISS
                     GENDLYENIIHMVLARIDGDPEGTKGLSLFLVPKILVHSDGSLGNRNDVICTGVEHKM
                     GIKASATCTLSFGDNDNCIGYILGKERDGIKNMFHMMNEARLDVGLEGLGVSSAAYMH
                     AVQYAKSRIQGKTISKNEPSIIGHPDVKRMLLWMKSHVEAMRMLVALIAYHIDISRVR
                     NDEIGKKAQALVEFLIPICKAGNTDLSWLITAEAVQVYGGYGYCGEYPVEQYARDSKI
                     LSIYEGTNGIQSIDLTMRKILSDEKRERYNAYKDYASDVIKKARAIVPENIINQVTIA
                     IAEMDSLIDVLAAKDTAFALSVATMLQQAFRILTHGILHLNSMTIAIQKLQSLAGGYT
                     YNQVPEGLEDNSEIAFYYGKVLSAQFFLTVEFEKYSGIIQGIRNENGVVLRAGSSIFT
                     GVLEA"
BASE COUNT          843 a          619 c          468 g          884 t
ORIGIN      
        1 gcatttggtt gtgtaatgga aattaattga ttaacttcat ctgctgtata tagaggattc
       61 attaatgtag caattccacc ggccataagt gtgccaaaat aagcaatagc aaattgcggt
      121 gaatttggta attgcaaaca aactctaccc ccttttttaa cgccaatttt taccagtgcc
      181 tgtgcaaatt ttattgataa cagccgcaac tggtaatagg taatttgtgt gccaaagaaa
      241 tccgttgcaa ctttatcagg tgcaacggcg gcactacaat gcaacatatc ctgtactgtc
      301 atcctctgaa agttaacaga atggtaccag aaatcataat tcttttccca taaacgctcc
      361 ataaaagtcc tccattttta ttgtaaagat ttataataat tacacagcaa aaagccagtc
      421 tgtatgccac tatagtaatc ctttaaaagc tgttgtttgc ttgtggttgt ccgtttagtt
      481 ttaaaccagg caggtgggca tatttctgtt atgcgtgtat cgtcaggagg attttcaata
      541 aattcaattg ccttattata atttactggc ctttggacta aagctttaac aaattcagga
      601 tagctcctga acaataattt cagtaaagta ccgctttcag atttgtcttt cctgtattct
      661 ttagcctgtg aacgtattac cacaatatcg cgtgcaccac gtttgtatgc ttccttgact
      721 ggaattgaat caccaacacc accatctgca actctttgac cttctataaa taatttattt
      781 ctatataata ctggtaatgc acttgaagct tttaacacaa attccagatt ttctttattt
      841 ggttttatat ataacgcttt ccctgattcc attgaagtta caacaacaag aaactctcta
      901 ctactatcct taaatatttc atgtaaattt aggcgatagt tactgatagt aatgtcccat
      961 agccagtcaa gatccaaaac atttccccag aaaatcctga aaggatttat aaacctccat
     1021 gatgctgacc agttttccat tatatagaag ttacgctcat actgcctgcc aaggaaggat
     1081 gctagattac atgcacctgc agacacacca atatacaaat caaaatgagt taggccacgc
     1141 aatcccattg caaaaagcac tcccgcagaa aaaacgccgc gcatgccccc accctcaaca
     1201 atgagtgctt ttttaccgcg cggcgttata tcggcattat ggatgtaaat ggacggatga
     1261 atttgcattg ttttacgctt caagaacacc agtaaagatt gatgaaccag cccgcaatac
     1321 aactccgttt tcattgcgga taccctgtat aatccctgaa tatttttcaa attccactgt
     1381 aaggaaaaat tgtgctgata gtactttgcc ataataaaaa gctatttcgg aattatcttc
     1441 taacccttct ggaacttgat tgtatgtata gccacctgcc agcgattgca acttctggat
     1501 tgctatggtc attgaattca ggtgcagtat tccatgtgta agtattctga aggcttgttg
     1561 caacattgtt gcaacactca acgcaaaagc tgtatccttt gctgcaagca catcaatgag
     1621 ggaatccatt tcagcaatag cgattgttac ctggttaata atattttcag gaactatcgc
     1681 ccgggctttt tttataacat cactggcata gtctttatat gcattgtacc gctcgcgctt
     1741 ttcatcagac aaaattttac gcatggtcag gtctattgat tgaatgccat tagtaccttc
     1801 atagatagaa agaattttgg aatcacgggc atattgttca acaggatact caccacaata
     1861 tccatagcca ccatatacct gaacagcctc agctgtaata agccatgata ggtcggtatt
     1921 acccgcctta cagatgggga ttaagaattc caccagtgcc tgtgcctttt taccaatttc
     1981 atcattacgt actcgtgaaa tatcaatatg atatgcaata agagctacaa gcattcgcat
     2041 agcctcaaca tgtgatttca tccataacag cattcgctta acatcagggt gtcctataat
     2101 tgaaggctca ttttttgaaa ttgtttttcc ctgaatacgt gattttgcat actggactgc
     2161 atgcatatat gctgcactgg aaacacctaa tccctccaat cccacatcaa gccgtgcttc
     2221 attcatcatg tggaacatgt tcttgatgcc atcacgttct ttgcctaaaa tatatccaat
     2281 acagttatca ttatcgccaa agcttagcgt acaggttgct gaagctttaa tacccatctt
     2341 atgttccaca ccagtgcata tgacgtcatt gcgattaccc aacgaaccat cgctatgtac
     2401 cagaatcttt ggaacaagaa atagggacaa tccttttgtc ccttctggat caccatcaat
     2461 acgagcaaga accatgtgta ttatattttc atacaaatcg ttttcaccag atgaaataaa
     2521 tatcttctgt ccttttattt tatatgttcc atctggttgc ctgtatgctt ttgttttaat
     2581 agctccaaca tctgaacctg cttgtggctc agttaaacac atggtaccac cccacctacc
     2641 agttaccata ttatgaagat acaattgttt ttgttcttca ttaccaaata tgcgcaccag
     2701 attggttgca ccgtttgaca gtgtaatata aatggtaaat gaaatacttg cggccagaaa
     2761 atactcaagt gctgatcgat acatcatctc aggcataccc ataccaccac aaga
//
LOCUS       DAOSZP010000287         2810 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_27922_length_2810_cov_3.234846, whole genome shotgun sequence.
ACCESSION   DAOSZP010000287 DAOSZP010000000
VERSION     DAOSZP010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2810
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27922_length_2810_cov_3.234846"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="PK348_06905"
     CDS             <1..203
                     /locus_tag="PK348_06905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HXK65979.1"
                     /translation="HIWSVDSNEVFLSCHVCISGGDSSLNTDSIIKAINGMLEEKFGI
                     THTTLQIEHTNLCTLEGGSCCR"
     gene            206..532
                     /locus_tag="PK348_06910"
     CDS             206..532
                     /locus_tag="PK348_06910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65980.1"
                     /translation="MSIYFTHPQLGQEVESIAGVYAYTEEKRLQIDEKDVLCFIGYSV
                     TNKSCCGVGGCMFATVAGFVQQYAVKKGEDNIVSMVEPITDTALRKQIEKLIKAQGVL
                     QVNFAD"
     gene            complement(529..951)
                     /locus_tag="PK348_06915"
     CDS             complement(529..951)
                     /locus_tag="PK348_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014185404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HXK65981.1"
                     /translation="MDYKHLTAPCGLDCFNCPMYLAGSDDNLRKKIAQSLNISFEKAV
                     CKGCRNEQGKIEFLHMQKTCSVYACTQSKGIDFCYECNDFPCDNLHPYADRASQVPHN
                     TKVFNLCLIKKMGLEKWAAEKAHSVKKTYFSGTFKLTD"
     gene            1357..>2810
                     /locus_tag="PK348_06920"
     CDS             1357..>2810
                     /locus_tag="PK348_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HXK65982.1"
                     /translation="MGAFKELTMWQALHGTVEKYPDNEALVYPEFGIRMTYAQFYQKC
                     REVAKGLIALGIQKGDHVSLWATNVPEWVYLQFSLGMIGGVLVTVNTNYKSHELEYLL
                     KQSDSTTLFMIEGYRDSNYYQIIREIIPNLDETQPGNINATNLPMLKNIVFIGKRDSL
                     PGMFTFDDIVLMGSSLSDAELDKRINECDIYDVINMQYTSGTTGFPKGVMLTHYNIVN
                     NAIMVGDVMGMTSSDRLLIHVPLFHCFGCVMSTLNCVTHGSTMVVLEYFDPLKSLQAV
                     QNEKCTAINGVPTMFIAMLNHPDFRKYDMSSLRTGIMAGAPCPVETMNQVRTIMHCPE
                     VVIAFGQTECSPVMTMTRRDDPVDLRVSTVGRLLPGIEGKIVDPETGEDLPPYTQGEI
                     VTRSACVMKGYYNMPEATTNAIDKEGWLHTGDLGSVDEHGYFKVTGRIKDMIIRGGEN
                     IYPREIEEFLLTNPKIKDVQVVGIPDEKYGEQVLA"
BASE COUNT          864 a          479 c          611 g          856 t
ORIGIN      
        1 tgcatatctg gtctgttgac tcaaatgaag tatttttatc atgccatgta tgtatcagtg
       61 gtggcgatag ttccctgaat acggattcca ttattaaggc aattaatggc atgcttgaag
      121 aaaaatttgg cattacccat accaccttac agatagaaca taccaatctg tgcactctgg
      181 aaggcggaag ctgttgcagg taaccatgag tatatacttt acccatccgc aattaggtca
      241 agaagtagaa tcaatagcag gtgtgtatgc atatactgaa gaaaaacgct tgcagataga
      301 tgaaaaggat gtgctgtgtt ttattggata ttctgtgacc aacaaaagct gctgtggtgt
      361 tggaggatgc atgtttgcta ctgttgcagg atttgtgcaa cagtatgctg tgaaaaaagg
      421 tgaagacaat attgtttcta tggttgaacc aataacggat acagcactac ggaaacaaat
      481 tgaaaagctc ataaaagcgc agggggtgct acaggtaaat tttgcagatt aatctgtaag
      541 tttaaatgtt ccgctgaaat atgtcttctt aacgctatgt gctttttcag ctgcccactt
      601 ttcaagtccc attttcttaa tcaggcataa attaaaaact tttgtattat ggggaacctg
      661 tgacgctcta tcagcatatg gatgaaggtt atcacagggg aaatcattgc attcatagca
      721 aaaatcaatc ccttttgact gagtgcaggc atatacgctg caggtttttt gcatatgtag
      781 aaattcaatc ttaccctgtt cattgcggca ccctttacat accgcttttt caaagctaat
      841 gttcagtgat tgtgcaattt tttttcgcag gttgtcatcg cttcctgcca ggtacatagg
      901 gcagttaaaa caatcaagac cacatggtgc ggtaaggtgt ttgtaatcca taagagaatc
      961 ccccgaatga gaattgtaaa gtaaagtgct atttattaca gttactatcg catagtataa
     1021 taccatagga gtttgtaaag ataaatatta attttcatgt tactcaataa atggttggac
     1081 atccaattaa ttattgacag aaaagaaggt atagtgtagt ggtacaaaaa atgatgatgc
     1141 tggataggca acggtggagt acttagtagc attattattt tgaaagcata aaaaatttat
     1201 caaaggagtt acatttcagc aaataataaa tttttatgat aaaatgttga catataatgg
     1261 catgatttta cagtttacgt taactggatt cattataagc attatagtat atttatatta
     1321 acatataatt ctttatataa gaaaggagag gttggtatgg gagcttttaa agaattaacc
     1381 atgtggcagg cgcttcatgg tacagttgaa aaatatcctg ataatgaagc actggtatac
     1441 cctgagtttg gcatacgcat gacgtatgct cagttttatc aaaaatgcag ggaagttgca
     1501 aaagggttaa ttgcactggg catacagaag ggtgaccatg tttctctgtg ggcaaccaat
     1561 gtgcccgaat gggtatatct tcagttttca ctgggtatga ttggcggagt gcttgttacg
     1621 gtcaatacca attataaatc ccatgaactg gagtatctct tgaaacaatc agattccacc
     1681 acattgttta tgattgaagg ataccgcgat agcaattatt atcagataat acgtgaaatt
     1741 atccccaatt tagatgaaac acagccaggc aatataaatg caacaaatct cccaatgctg
     1801 aaaaatattg tttttattgg gaagcgggat tcattaccag gtatgtttac gtttgatgat
     1861 atagttctta tgggaagttc tttaagtgat gctgaactgg ataagcgtat taacgaatgt
     1921 gatatttatg atgttatcaa tatgcagtac acatccggta caacaggatt tcccaaaggt
     1981 gttatgctta ctcattacaa tattgtcaac aatgctatca tggtgggcga tgtcatgggt
     2041 atgacttcca gtgataggct gcttattcat gtcccattgt tccactgctt tggctgtgtt
     2101 atgagtacat taaactgtgt aacccatggt tcaacaatgg tggtactgga atactttgat
     2161 ccgttgaaat cgttgcaggc agtacagaat gaaaagtgta cagcaattaa tggggtcccc
     2221 acaatgttta ttgcaatgct caatcatcct gattttcgca aatatgatat gtcatcactc
     2281 agaaccggca ttatggcagg agccccgtgc ccggttgaaa caatgaatca ggtgcgaact
     2341 attatgcatt gtcctgaagt tgttattgct tttggccaaa ctgagtgctc accagttatg
     2401 accatgacgc gccgtgatga tccagttgat ttgcgggttt caactgttgg tcgcctcctt
     2461 ccaggtattg aaggtaagat agttgatcct gaaacaggtg aagatttacc accctataca
     2521 cagggtgaga tagttacccg aagtgcatgt gttatgaaag gctattataa catgcccgag
     2581 gccactacca atgccattga caaagaaggc tggctacaca ctggcgatct gggttcggtg
     2641 gatgaacatg gctactttaa ggttaccggg cgtatcaaag acatgattat ccgtggtggc
     2701 gaaaatatct atccacgcga aattgaagaa tttttgttga ctaatccaaa gataaaagat
     2761 gtacaggtag taggtattcc tgatgaaaaa tacggcgaac aggtacttgc
//
LOCUS       DAOSZP010000288         2798 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_28052_length_2798_cov_2.619030, whole genome shotgun sequence.
ACCESSION   DAOSZP010000288 DAOSZP010000000
VERSION     DAOSZP010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2798
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28052_length_2798_cov_2.619030"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2021
                     /locus_tag="PK348_06925"
     CDS             <1..2021
                     /locus_tag="PK348_06925"
                     /inference="COORDINATES: protein motif:HMM:NF019368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HXK65983.1"
                     /translation="YLTSPDTSARVLVWHTVGLNGYGENFDLNMRLSLPFKDEEFVSM
                     ENSIAYHLAQLEKGYPTEEIQRYYDYDHKHWDYPYDAYLLPYYPAQGGDNQPQNIVPS
                     EIAKTWNNKFINPKIIIATPHEFFDYISQKYANNIPVLKGEMPPFWGEQIYLDFIQVD
                     PERLIINNWFDRALYARGKDLVDALLQNSTVDVNSYNLQSLEGYMAIILNNDHNPRPV
                     PFGKTHYTKQDVKDWMNTRNEWVYAPLHSIKDFKLLQENKENKQWRQIHYDANKPIII
                     ENQFYRISFDADKGCIKSILDKQSQKEWVSKNHMYGFNQYVIGIRGENAARRNYFETI
                     AGFKKVTTTLYNNGNDYRIVVEGSEHAYYRGMDILSEFFNNAFGIHVPPVILKIAYFF
                     YQLFTPSLSITQEIIIPAGEKRIDFIQHVKGKAPQIAEHYFAYPVNSREMLYDSAYTV
                     LRWGSIDTGGNLIPAAKNIAPFKSINDTLYPFQWMHGLPSSVYFDSFVLHKEGSKYAV
                     FVSQDSKAIVPVKQDDMAGFYHCCIGWTLWGKLGLGRDLPDTIAFKSTFTSFTAQTHN
                     EAIATAYRFSFDESGFIQSPCIQTNNTHVAIIYNQPISQNKIVIGMYELLGKKQQAAC
                     IINTKRAIQKAYYSDVSGKHIKPVAVKNYSIEITLNPHELSFITLELL"
     gene            2034..2738
                     /locus_tag="PK348_06930"
     CDS             2034..2738
                     /locus_tag="PK348_06930"
                     /inference="COORDINATES: protein motif:HMM:NF015682.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HXK65984.1"
                     /translation="MAPVYSYEETVELCKRLEKTYTPAVCDTLDEMGFMHQAMCSGFT
                     PIMPKAAIAGPAFTMEEAKTRKSTRLKDYDPEFVAKVLEDLFGSMQKGQVVAVNANDF
                     YGAGAWGELMSTTCKYFGGVKGAVVDGPIRDINRILEIEFPVWARGNIPTDSIGRVDL
                     VGMGAPIWCGGVRVNPGDIIFADCDGVVVIPVKDVDLKEVVEKAEEVVQAERRSRQEI
                     RQGMSLVDVYHKYGKL"
BASE COUNT          884 a          493 c          634 g          787 t
ORIGIN      
        1 tttatcttac ttcacctgat acaagtgcaa gggttctggt gtggcatact gttgggttaa
       61 atggctatgg tgaaaatttt gatttgaata tgcgcctgtc cctccctttt aaagatgaag
      121 aatttgtaag catggaaaac agtattgcat atcatttagc ccagcttgaa aaaggctacc
      181 ccacagaaga aatacagcgc tactatgatt atgatcataa acactgggat tatccctatg
      241 atgcatacct tttgccatat tatcctgcac agggtggtga taatcagccg caaaatatag
      301 tcccttcaga aatagctaaa acatggaaca acaagtttat taatccaaag attatcattg
      361 caacacctca cgagttcttt gattacattt cacaaaagta tgcaaacaat atcccagtac
      421 tcaaagggga aatgccacca ttctggggtg agcagatcta cctggatttt atacaggtag
      481 acccagagcg cctgattatt aataattggt ttgatagagc actatatgcg cgtgggaaag
      541 accttgttga tgctttgctg caaaacagta cggttgatgt aaatagctat aacctgcaga
      601 gtcttgaagg gtatatggca attatactca ataatgatca caatccacgt ccggtgcctt
      661 ttggaaaaac ccattataca aaacaggatg ttaaagactg gatgaataca cgcaatgaat
      721 gggtatatgc ccccctgcac agtatcaaag atttcaagct tttgcaggag aataaagaaa
      781 acaaacagtg gagacagatt cactacgatg caaataaacc aataattata gaaaatcaat
      841 tttacaggat atcatttgat gcagacaaag gatgtatcaa aagcatactt gataagcaat
      901 cgcagaagga gtgggttagt aaaaatcata tgtacggatt caaccagtat gttattggca
      961 ttcgtgggga aaatgctgcc aggcgcaatt actttgaaac aattgctggt ttcaaaaagg
     1021 ttactaccac cctgtataat aatggtaatg attatcgcat agttgtagaa gggtcagagc
     1081 atgcgtatta caggggcatg gacatacttt ctgaattttt taacaatgcc tttggcatac
     1141 atgtgccacc tgtcatattg aagatagcat actttttcta tcaacttttt acaccgtcac
     1201 tgtcgattac gcaggaaatt atcatccctg caggcgaaaa gcgtattgat tttatccagc
     1261 atgttaaagg taaagctcct cagatcgctg aacactactt tgcttatcct gtaaactcac
     1321 gagaaatgct atatgattct gcatatacag tattgcgatg gggatcaata gatactggcg
     1381 gcaatcttat acctgctgca aagaacatag cgccattcaa aagtataaac gatacattgt
     1441 atcctttcca gtggatgcat ggtttgcctt catcggtata ttttgactcg tttgttttac
     1501 ataaagaagg ttcaaagtat gcagtatttg tgtcacagga cagcaaagcg atagttcctg
     1561 tcaaacagga tgatatggca ggcttttacc actgctgtat tggatggaca ttatggggga
     1621 aattggggtt gggtagagat ttgccagata ctatcgcctt taaaagtaca ttcacaagtt
     1681 ttactgcaca aacacacaat gaagcgatag caactgcgta caggtttagt tttgatgaat
     1741 caggttttat tcaatcacca tgtatacaaa caaacaatac acatgtagct ataatctaca
     1801 atcaacccat ttcgcaaaac aagatagtca ttggtatgta tgagctatta ggcaagaagc
     1861 aacaggctgc atgtatcatt aatacaaaga gagcaataca aaaagcgtat tattcagatg
     1921 tttctggcaa acatatcaaa cctgttgcag taaaaaacta tagtatagaa ataactctga
     1981 atcctcatga gttatcgttt ataacattag aattattata atggaggtaa tgtatggcac
     2041 cagtatattc atatgaagaa accgttgagt tatgcaaaag gttggaaaag acgtacacgc
     2101 cagcggtttg tgacacactt gatgaaatgg ggtttatgca tcaggcaatg tgctcagggt
     2161 ttacgcccat aatgccaaaa gcggctattg cagggcctgc gtttaccatg gaggaagcaa
     2221 aaacgcgcaa aagcacacgg ctaaaagact atgatccaga atttgttgca aaagtgctgg
     2281 aggacttatt tggaagcatg caaaagggac aggttgtggc ggttaatgca aatgactttt
     2341 atggtgcagg tgcgtggggt gagctaatgt caacaacctg taaatacttt ggtggcgtaa
     2401 agggtgccgt ggtggatggt ccaattcgtg atattaatcg cattctggaa attgaatttc
     2461 ctgtctgggc acgaggcaac atccctactg actctattgg cagggttgat ctggttggta
     2521 tgggtgcgcc aatatggtgt ggtggtgtgc gggtaaatcc aggtgatatt atttttgctg
     2581 actgtgatgg cgttgtggtc atccccgtaa aggatgtaga tttaaaagag gtggttgaga
     2641 aagctgaaga ggttgttcag gctgaacgcc gttcacgaca ggaaatacgg cagggcatgt
     2701 cactggttga tgtgtaccat aagtatggga agctgtgaag agggttattg acatgaaaac
     2761 agtagttgtt acggggtgtt caggatatgt tgggcaac
//
LOCUS       DAOSZP010000289         2785 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_28205_length_2785_cov_2.960440, whole genome shotgun sequence.
ACCESSION   DAOSZP010000289 DAOSZP010000000
VERSION     DAOSZP010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2785
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28205_length_2785_cov_2.960440"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(179..643)
                     /gene="ribE"
                     /locus_tag="PK348_06935"
     CDS             complement(179..643)
                     /gene="ribE"
                     /locus_tag="PK348_06935"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015042627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HXK65985.1"
                     /translation="MNTIEGKLDASGLKVAIIVARFNEFITNKLLSGALDCLQRHNAD
                     EKNITVIWVPGAFEIPPVAMKVATKGGYDAVICLGAVIRGATPHFDYVAAEVSKGVAL
                     VSLQSQVPVIYGVLTTDTIEQAVERAGTKSGNKGFDAAMAAIEMAQLYKKIS"
     gene            complement(752..2029)
                     /locus_tag="PK348_06940"
     CDS             complement(752..2029)
                     /locus_tag="PK348_06940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65986.1"
                     /translation="MVEKLIRYILLAAVISISMVLFAQDKPQEKKSSIGKTIKKTIET
                     IDKQTLYKEKKETEQDKAEKKQIQTYQQADFDKLKDATHEDISTIIVLLGEIPLESKN
                     SIFAPAILYNKNNIFNSNTDFAFTWVGFKATAKFTQKKFIWDNVSLQETLIGSFLYAS
                     GTNFGFFGERFNEDLLFYTNYTSEIVTLKISLPLYNILGLTIDSRQYFFIKRDTPPDF
                     IMPKNHYNLFPRIDWNIEHYTEQGIDQLFNGIAFQNWIGYGIRSQWDTWGEPGKLQMG
                     KEAKTFVIYSSTLTCGKVFGNSQNLIVRMRIKGGIDNDFLSQPRFGGTIDNAKLDVVH
                     GTTVDQFRVESFALCNVQYGFNVATRLRMNLYVDYAYIINPASQHIAGSGYGFRILGP
                     GGLPIWLTHGISKNLSQDDNVSQVVMIMTAAGF"
     gene            complement(2161..>2785)
                     /locus_tag="PK348_06945"
     CDS             complement(2161..>2785)
                     /locus_tag="PK348_06945"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HXK65987.1"
                     /translation="EFLTLTQKGKKLALLETYSAKDVAPAPLPLFIKNCKYLRSSIPA
                     LAKQLDYCISLIDNTQSAKEDPVTQTIQILTFLKQNALFKDTPVDQIVHLIQIARIQK
                     LPENTEFIKEGQTGDELFIIMEGEVSVSKKGKEIARLGIGDCIGELSIIDKEPRSATV
                     KTTRQSLLLSLKRNDFLLTVKSNPVIAINVMKVIADRLRSILSQYNI"
BASE COUNT          804 a          556 c          507 g          918 t
ORIGIN      
        1 ataaaatcta aatgatctat agtgccttca attaatgtaa tggcaaaatc cctgatatcc
       61 tccggaattt ctttatctac ccattgcagg gaaattagct cctgtactgg tgtacgaacc
      121 gtttcgtaca tgtacaaggc ctgcatggca tattcacggg cttttcttct gtgacccatc
      181 agcttatctt tttatacagt tgtgccatct caatagctgc cattgcagcg tcaaagcctt
      241 tattgcctga tttggttcct gcacgttcaa cagcctgttc aatggtatcg gttgtaagaa
      301 ctccatagat aaccggcacc tgcgattgca gcgatacaag cgcaacacct tttgaaactt
      361 ctgctgcaac atagtcaaaa tggggagtag caccccgtat aactgcgccc aggcaaatca
      421 ccgcatcata accacctttg gtggctacct tcattgccac aggaggaatt tcaaacgcac
      481 cgggtaccca aataaccgtt atgttttttt catcagcatt gtgccgttgc aggcaatcta
      541 aagcgccaga taaaagttta ttggtaataa attcattaaa acgtgcaact attatcgcaa
      601 cctttaaacc ggatgcatct aatttccctt caattgtgtt catagttaac ctgcttatat
      661 aataaatttt aatttgggct aaagcttata ataataaatc taatgcatga caaagtttgt
      721 ttcaatatat tctattccgt caagttattt tttaaaaacc tgcagcggtc atgatcataa
      781 cgacctgact cacattatca tcctgtgaca gatttttgct tataccatgt gtcagccata
      841 taggaagtcc cccaggtccc agtattctga agccatagcc gctgcctgca atgtgttgtg
      901 atgcagggtt tataatataa gcataatcaa cataaagatt catccgtaac cgtgttgcca
      961 cattaaatcc atactgcacg ttacacaggg caaaagactc tactctgaac tgatccaccg
     1021 tggtgccatg taccacatcc aatttggcat tgtcaatggt tccgccaaag cgtggctgag
     1081 ataaaaagtc attatcaatg ccacctttaa tgcgcatgcg tacaatgagg ttctggctgt
     1141 taccaaagac tttgccacag gttaatgttg atgaataaat aacaaaggtt ttagcttctt
     1201 tccccatctg aagcttgccg ggttcacccc acgtgtccca ctggctgcgg attccatatc
     1261 caatccagtt ctgaaatgca atgccattaa ataattggtc aatcccctgt tcggtatagt
     1321 gctcaatatt ccaatcaatg cgcggaaaaa gattatagtg gttttttggc ataataaaat
     1381 cagggggtgt atctcgcttt ataaagaagt attgccggga atcaattgtt aagcccagga
     1441 tgttatataa tggcaaagaa atttttaagg taactatctc tgatgtgtag tttgtataaa
     1501 acaggaggtc ttcattaaag cgctcgccaa agaaaccaaa gtttgtacca ctggcatata
     1561 aaaatgatcc aattaatgtt tcctgtaatg aaacattatc ccatataaat tttttctggg
     1621 taaattttgc agtagctttg aacccaaccc aggtaaaagc aaaatccgta tttgagttaa
     1681 aaatattgtt tttgttataa aggatagcag gggcaaaaat actattttta gattccagtg
     1741 gtatttcacc cagcaatact ataatggttg agatatcttc atgggtagca tccttcaatt
     1801 tatcaaaatc agcctgctgg tatgtttgta tttgtttttt ttctgcttta tcctgttcgg
     1861 tctctttttt ttctttgtat aatgtttgtt tgtcaattgt ttcaattgtt ttcttaatag
     1921 ttttgccaat agaagatttt ttttcttgag gtttgtcctg tgcaaatagt accattgaaa
     1981 tggaaatgac tgcagctaac agaatgtatc gtattaattt ttccaccatg aattcctgaa
     2041 agttgtaatt ttgtaattta tttacaatac ctgtttacga tatacctgcc atcactggtg
     2101 acaggattgt acctttcatt atctaagaca attgtataaa aaatacatgc tgcattgtat
     2161 tcatatattg tattgtgata atatggatct gaggcgatcc gcaataactt tcatgacatt
     2221 gatagctatc acaggatttg atttcaccgt taataaaaaa tcattgcgct ttaatgaaag
     2281 taaaagggat tgcctggtag ttttcacggt agcactgcgg ggctctttat ctataatgga
     2341 taactcacca atacaatctc caattcccag acgcgctatc tcttttcctt ttttgctgac
     2401 actgacttcc ccctccataa ttataaaaag ctcatcacca gtttggcctt ctttgatgaa
     2461 ttcagtattt tcaggaagct tttgtatacg tgcaatctgt atcagatgaa caatctggtc
     2521 aacaggtgta tctttgaaca gtgcattctg tttcaggaag gtaagtatct gtattgtctg
     2581 tgtcactgga tcctccttgg cgctttgagt attatcaatc aatgaaatac aatagtcaag
     2641 ctgttttgcc agcgcaggta tacttgaacg caggtattta caatttttaa taaaaagagg
     2701 tagaggtgca ggtgcaacgt cctttgcaga ataggtttca agtaatgcta gctttttgcc
     2761 tttttgcgtc agagtcaaaa attca
//
LOCUS       DAOSZP010000290         2769 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_28405_length_2769_cov_3.047163, whole genome shotgun sequence.
ACCESSION   DAOSZP010000290 DAOSZP010000000
VERSION     DAOSZP010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2769)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2769)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2769
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28405_length_2769_cov_3.047163"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..317)
                     /locus_tag="PK348_06950"
     CDS             complement(<1..317)
                     /locus_tag="PK348_06950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65988.1"
                     /translation="MKKLLPVLIVLFTTIANSQTYLYELSDNIETYKNKQITMILKLK
                     RYDTVFQVITFYDNKNHDITFDCIPLVKQPWFIQQRLNLHPGMEYTVTFTVKGVGNLK
                     EVIA"
     gene            complement(943..>2769)
                     /locus_tag="PK348_06955"
     CDS             complement(943..>2769)
                     /locus_tag="PK348_06955"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HXK65989.1"
                     /translation="KIVIIGDAAKEVSQPVFLGVLIIMLVYVPVLSLTGIEGKMFRPM
                     AETVLYALGGSIIIALFLMPVLAYYSLSPKKTDKEPFVFRVLEKVYNPFLTFSLKHPV
                     KVIIPTLLVALISIIMFIHLGSDFIPRLDEGDMVIGLVRDQTISLDSSLEIQKKSDQV
                     ISQFKEVKTVFSRMGTPESATDPMGVNFADTFVILEKDKSKWGLYNGKHPTKVELFEA
                     IALKIDEQVPGQEISLTQPIEMRFNEILEGNRADITLRIIGQNLDTLYELSNKSIPIL
                     EKIEGASTVELDPLTALKKSEIIDVVLNYDAISKYGVNINDVNNAFSIAMSGKQVGYL
                     YDEDKRFPIMIRISDVFRNNVANIAKIPVGLADGGAIPIGTVASVQRHQQITTIARTR
                     AQRYAAVSINIKNRDTQSFVNEAKEKIKALNIPKGYQLQWGGQFKNLESARLRLLIII
                     PIVLAIIFLILIRTFKSLTQTLLVFNSIPFALTGGIMLIYIRNIPLSVSAGIGFIALI
                     GIAILNGMVLVNFFNQLRGQGVKLEKAVYEGTLIRLKPVLMTALVASFGFIPMAFNTG
                     IGAEVQRPLATVVIGGLISSTILTLLLLPTLYLWVEKLFGKE"
BASE COUNT          870 a          552 c          427 g          920 t
ORIGIN      
        1 gcaatcactt ctttaagatt acccacaccc ttaacggtaa atgttacagt atattccatt
       61 ccaggatgaa ggtttaatct ttgttgtata aaccatggtt gtttaaccaa tggaatacag
      121 tcaaatgtaa tatcatgatt tttgttatca taaaatgtta taacctgaaa taccgtatca
      181 taccgtttta gttttaatat cattgtgatt tgcttatttt tatatgtttc aatgttgtcc
      241 gatagctcat aaagataggt ttgtgaattt gctattgttg taaataatac tatcagtact
      301 ggtagtaact ttttcataac atttcccatg aaaatagaat gcattgacca cttagccatc
      361 tcatgtgttc cagatttgtt acaatctaac ataaaattat tatccaactg tttctactgc
      421 catgttctaa tccatttaat tccagatttt ctgacctttg actgcaggtg ttatatttct
      481 tgttcaacca ggcttgttaa cttaatacat agtatttaat ttctctgtaa tgtctatcac
      541 tattagtaac tatcgtgcaa aaaaaactgc agctaaaaag ccgcagtaaa ataaaaaaac
      601 tgggattgat tgttatgaat aaacggattg tatcctgccg gatatgctac attaatgacc
      661 tcttttctga cttctataaa caatatattc aataatgacc caaagaagta cacataccca
      721 gaaaagcaca aaaacctgta taattggtat attcatcatg gttatgctcc ttagcttttc
      781 taatcatata aattagcaaa atttgtgcca acttttcttt tccgggaaac atttgaaagt
      841 gctggaattt cgagagtttt ctattattta tctatttctg gagttacaat gaagtttact
      901 atttatgcta caccgtgtca tttttgcaac acacagagtt tcttattcct tgccaaacag
      961 cttctccacc cacaaataca gcgttggcaa taacaagagc gtcagaattg ttgaagaaat
     1021 caaccctcct ataaccactg ttgcaagagg gcgctgaacc tcagctccta tgcctgtgtt
     1081 aaaagccatg ggaataaagc caaaactagc aacaagggct gtcatcaaaa ctggttttaa
     1141 acgaattaat gtgccttcat acacagcttt ttctaacttt acaccctgac cgcgtaactg
     1201 attaaaaaaa ttaacaagca ccattccatt gagtattgct ataccaataa gtgcaataaa
     1261 acctattcct gcagaaacac tgagcggaat attacggata tatatcaaca taattccacc
     1321 cgtaagagca aaaggtatgc tattaaatac aagtaaggtc tgtgttaaac ttttaaatgt
     1381 ccgtattaat attaaaaata taatagccaa aactatagga attattataa gtaaccgtaa
     1441 acgtgctgat tctaaatttt taaattggcc tccccactgt agttgatacc ctttagggat
     1501 gtttaacgct tttatttttt cctttgcttc atttacaaaa ctctgtgtat ccctattttt
     1561 gatatttatt gaaactgctg catatcgttg tgctcgtgtt cgcgctattg tcgttatctg
     1621 ctggtgtctt tgcactgaag ctacagtacc aatcggtatc gctcctccat cagccagacc
     1681 aactggtatt tttgcaatat ttgcaacgtt gttacgaaaa acgtcgctta ttcttatcat
     1741 aattgggaag cgtttatctt catcataaag ataacccacc tgtttaccac tcattgcaat
     1801 tgaaaaagca ttgtttacat cattaatgtt aacgccatat ttcgatatag catcataatt
     1861 gagaactaca tcaataattt cgcttttttt caatgctgtt aaaggatcaa gttctaccgt
     1921 agaagctccc tcaatttttt caagaatagg aattgattta ttcgatagtt catataacgt
     1981 atccagattt tgaccaatga ttcgcagggt aatatctgca cggttgcctt ccagtatctc
     2041 gttgaaacgc atttcaattg gctgagtgag tgaaatttcc tgtcctggca cctgttcatc
     2101 gatctttaga gctatcgcct caaatagctc aactttcgtt gggtgttttc cattgtataa
     2161 tccccacttt gatttgtcct tttccagaat gacaaatgta tcagcaaaat taactcccat
     2221 tgggtcagta gccgattcag gcgttcccat acgcgaaaat acggttttga cctctttaaa
     2281 ctggcttatt acctggtcac tttttttctg tatctcaagt gaagaatcaa ggcttattgt
     2341 ctgatcacga acaagcccaa taaccatatc accttcatca agcctgggaa taaaatcaga
     2401 tcccaaatgt ataaacataa taatagagat aagagctacc aacaatgttg ggataataac
     2461 cttcacagga tgctttaaag aaaatgttaa aaaaggatta tacacctttt caagaaccct
     2521 gaaaacgaat ggctctttat ctgtcttttt agggctcaaa ctataataag ccagaactgg
     2581 cataagaaac aaagctatta tgatactgcc accaagtgca tacaataccg tttcggccat
     2641 tggtctgaac attttgcctt caatacctgt gagacttaac actggcacat acaccagcat
     2701 aattattaag actcctaaga aaacaggctg gctcacttct tttgcggcat cacctattat
     2761 aacaatctt
//
LOCUS       DAOSZP010000291         2763 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_28467_length_2763_cov_3.037297, whole genome shotgun sequence.
ACCESSION   DAOSZP010000291 DAOSZP010000000
VERSION     DAOSZP010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2763
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28467_length_2763_cov_3.037297"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..764)
                     /locus_tag="PK348_06960"
     CDS             complement(<1..764)
                     /locus_tag="PK348_06960"
                     /inference="COORDINATES: protein motif:HMM:NF013056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HXK65990.1"
                     /translation="MTSYIEKYFDIQNNNTTISTEIRAGIATFLTMAYIIVVNPLILK
                     DAGMPFHAVLFATVMVSCISSMLMGLVANLPFALAPGMGINAFFTYTLVGSGTPWQVA
                     LGAVCISGIIFMLLSSWGIREKIVAAIPLHLRYAVAAGIGLFLGFIGLKNAGVIISHP
                     ATLVTFGGMGIQQLLFACGLIVTGVLVYRKIKGALIIGIIVTAALVWGTSHIYTLMGL
                     NPFAALPSEYSAYPDTSLFLNFNIRDALSVSLIVPI"
     gene            1573..2133
                     /locus_tag="PK348_06965"
     CDS             1573..2133
                     /locus_tag="PK348_06965"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HXK65991.1"
                     /translation="MELQQAIVERRSIRRFTDYYVTDDEIKQILEAARMAPSWANVQP
                     WEFIVVRDKALIEKITETYSPNNPARKCSLASTALLVACARKNISGCKEGQQVTALPN
                     WYMFDVGMAVQNLCLKAHELGLGTVVVGFLDHQKCNEILNVPPDSTVVVTIPVGKPEV
                     SGKQGPPRKELTEFVYLDVYGNKFFK"
     gene            2142..2603
                     /locus_tag="PK348_06970"
     CDS             2142..2603
                     /locus_tag="PK348_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011023482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HXK65992.1"
                     /translation="MDIKDKWIYFAGPLFTAAERQFNELLSQYIEQAGYAVYLPQKEC
                     QGISSANDIFRHCIKGLDNAWCVVALVDGADTDSGTCFEMGYAYAKGIPIIGLRTDFR
                     KSADDGHVNLMLSQSVATLCFVNSLETISIEEIGYKLKDEISRMLVKVIKS"
BASE COUNT          870 a          479 c          579 g          835 t
ORIGIN      
        1 aagatgggga caataagact taccgatagc gcatcgcgaa tgttgaagtt caggaaaagc
       61 gaagtatcag gatatgcact gtactcagag ggcagggccg caaatggatt aaggcccatc
      121 aaggtataga tatggcttgt accccaaacc agtgctgctg ttacaatgat gccaataata
      181 agtgctcctt tgatttttcg atataccagt acgccagtta caataagccc gcaggcaaag
      241 aggagttgct gtattcccat gccgccaaaa gtaaccagtg ttgcaggatg tgaaataatt
      301 acaccagcgt ttttcaatcc aataaaccct aagaaaaggc caatgcctgc tgcaacagcg
      361 tagcgcagat gtagtggtat tgctgctaca atcttttcac gtatacccca tgatgaaagc
      421 agcatgaaga taatacctga tatacataca gcacctaacg caacctgcca gggtgtgccg
      481 cttccaacca gcgtataggt aaaaaatgcg tttattccca tgcctggtgc cagtgcaaag
      541 gggaggttgg ccaccaatcc catgagcatt gaactaatac atgataccat aactgtagca
      601 aaaagcactg catgaaatgg catccccgca tcttttaaaa tgagcggatt aactacgatg
      661 atgtatgcca tggttaaaaa tgttgcaatg cccgcacgga tttcggtgct tatggttgtg
      721 ttattgtttt gaatatcaaa atatttttca atatatgatg tcataatctc cctcacgaaa
      781 tatgatatcg tattggttaa gattcttcct ttagataaat ggttgctgag tttagtgaag
      841 cattcagtgt ccacccttcg attttcatcc taagtaccaa ttaaattagt acaaagcatc
      901 tactctgtca tcctgagccc tgatgaaatc ggggcaaagg atcttgtctt ttgacacaca
      961 gttattgcaa tagaactgaa actgacgccc tgcgttcgca gtgacatgta actaccgtca
     1021 tcgttagtta cgataaagtc gagataatca attttatttt ttacttaaca tttcataaat
     1081 tctattcaaa catgttgtta caaaacacta caaagcatat ttgtagatcc tttctattag
     1141 ctgcacaaac accttgtgac aaattcaaaa cccagtcgag cttcagttgc caagcttatt
     1201 gaaacacgag tgttaacaat ggcaatagag aagttttttt aatcctttat aaaaggaatg
     1261 gggtagttat taaattgcaa gaaaaatttt actaattgca ttgtaaacac tacaatacat
     1321 gaagatgttg ctcatacttc aattggtagt attaattatg gataaatata ctgaccaact
     1381 atgaaactaa aagagaataa aaacgttggg taatgataca gacatcatca ttgattttaa
     1441 tgtaattgaa ttatactgga aaaatcgtat ttatacataa ttttatataa aataaaattg
     1501 ctttacatta ttattatttt tctgtagctt tgccatacag gctattattt ttttgtatat
     1561 aaagaggtgg ttatggaatt acaacaagcg attgtcgaaa gaaggagtat tcgccgcttt
     1621 accgattact atgtaactga tgatgagatt aaacaaatac tggaggctgc ccgcatggct
     1681 ccttcatggg ctaatgtgca gccatgggaa ttcattgtag tgagggataa agcattaatt
     1741 gaaaaaataa ctgaaaccta ctcacccaat aatcctgctc ggaaatgttc actggcatca
     1801 actgcgctgc tggttgcgtg tgcccgtaaa aatatatcgg gctgcaagga aggtcagcag
     1861 gtgactgctc tgccaaactg gtatatgttt gatgttggta tggcggttca aaatttgtgc
     1921 cttaaagcac atgagcttgg gcttggcacg gtggttgttg gatttcttga tcaccaaaag
     1981 tgtaatgaaa tccttaatgt tccacctgat tcaacggttg ttgtaacaat cccagttggg
     2041 aaacctgagg taagtggcaa acagggacca ccacgtaaag aattaactga atttgtgtat
     2101 ttagatgttt atggcaataa atttttcaag taaggtatat catggatata aaggataaat
     2161 ggatttattt tgcagggcca ctatttactg cagcagaaag gcagtttaat gaattgttat
     2221 ctcaatatat tgagcaggcg ggttatgctg tgtatctacc gcaaaaagaa tgccagggaa
     2281 tttcttcagc caatgatata ttcaggcact gcataaaggg acttgataat gcgtggtgtg
     2341 ttgttgcact ggtggatggt gccgatactg attctggcac atgttttgaa atgggatatg
     2401 cgtatgcaaa gggtattccc ataataggtt tgcgaacgga tttccgaaaa agtgctgatg
     2461 atggacatgt gaacctgatg ttgtcgcaaa gtgttgcaac gttatgcttt gttaatagcc
     2521 tggaaactat atctattgaa gaaattggat ataaactgaa agacgaaatc agtagaatgc
     2581 ttgttaaagt aattaaaagt taacatgcta cagtggttta tttgttgtgg cttatttgtt
     2641 tttttagctg tgatgtgcac atagatacct gatcacctat attctgcttt ataagatacg
     2701 aagtcaattt ctggagggat taacagtgaa aaaaatagta gtatatagtg ccgtttttat
     2761 tgg
//
LOCUS       DAOSZP010000292         2748 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_28667_length_2748_cov_3.327144, whole genome shotgun sequence.
ACCESSION   DAOSZP010000292 DAOSZP010000000
VERSION     DAOSZP010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2748)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2748)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2748
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28667_length_2748_cov_3.327144"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1383
                     /locus_tag="PK348_06975"
     CDS             <1..1383
                     /locus_tag="PK348_06975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65993.1"
                     /translation="TIFNYCVVLYNALASSCLIDKQYIELKWTRPEGFKQLLSPYMNS
                     MLKLSLCDAQTKEAMIQLIDQKLRQSQDLQKNETMNKKNLTIEQYIYDFITTLRCLLY
                     PSTTNECSLTDLLKKEGSLPSFEAFCIACVEALVIGKPVVLSDIMTMFDITAPKVSDT
                     EWNFDHDELVKAGKDPESLKKKAKEELHRKLEPYDELYRFLTYAPEGKEVAIQGFDDQ
                     RKLVDKRNEVFDDAYSKDILIALEGMLLGFINEYGALLDGTTIYFENNKNHYEGCIFT
                     ENYFSHELARFSEILSEINTFRSNNPTFHVTRDELIEGINKKKKSLYPIERFMTVIGD
                     MCYDIGIELFTLYNRHRRWVLSGSSGKDDAVMRTAFSAHTSGLSSLDDREAMPVPFSD
                     CIITGFSKLRPLSKLLLHKPVIHPTLRDGVFPQMVAYLLQMAHVCLNDRLYGILDERT
                     KILSQIRSLS"
     gene            1380..2573
                     /locus_tag="PK348_06980"
     CDS             1380..2573
                     /locus_tag="PK348_06980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65994.1"
                     /translation="MRLLVILAMLSFALRAEAAPRVLVFKSYHDNLTSLLDEYRIPYI
                     VKRASVVAGDSSLSGFDAVFIPCGVEQPIEASIQVMAHGQRIQGVALKDDYEGIDFKR
                     LNALLQDFVKSGGRLYAAGYSYRFVEALGCSLSFFNNFPHYGTSGRIHSFLNDDFASF
                     VMRDDMQLTVNYNGWVALQRANASILAKGYFPTVGGEREGPVAFFKKYGNGVVYYSAY
                     HTGEDSLYMRYFIFRTAYGMLVDELLQKAWYWGQSIALSVVDTFKGDEYVRSYTCTLP
                     QGAITIYLPTTYPVQVDIFANKKLLGSWDSGFITEISLYNPATQDATVYVYQSNRAAY
                     KPFALVIAQGRRILPYMPVVRLSMGAIAVIMLLGFTWKRINPRKFAGKRRYWFRDGHF
                     IHRQK"
BASE COUNT          791 a          510 c          613 g          834 t
ORIGIN      
        1 acaattttta attattgtgt tgtactgtac aatgcgcttg catcgtcatg cttgattgat
       61 aagcagtata ttgaacttaa atggacccgg cctgaggggt ttaagcagtt actatcgccc
      121 tatatgaatt ctatgctcaa gctttccctg tgcgatgctc agaccaaaga agcgatgatt
      181 caattaattg accagaaatt gcgtcaaagc caggatcttc agaaaaatga aacaatgaat
      241 aaaaagaatc tgaccattga acaatatatt tatgatttca tcaccacact gcgatgtttg
      301 ctgtatcctt ctaccaccaa tgaatgttca ctgactgacc ttcttaaaaa agaaggatcg
      361 ttgccatcat ttgaagcatt ctgtattgcc tgtgttgaag cgctggttat aggcaaacct
      421 gttgtgctat ctgatatcat gacaatgttt gatattactg ctcccaaggt ttcggatact
      481 gaatggaatt ttgatcacga tgaactggta aaggcaggga aggaccccga atcgttaaaa
      541 aagaaagcaa aggaagagct gcaccgaaag cttgagccgt acgatgaatt gtacaggttt
      601 cttacctatg ctcccgaggg gaaggaagtt gccattcagg gttttgatga tcagcgtaaa
      661 ctggtagata agcgcaatga agtgtttgat gatgcataca gtaaagacat actcattgca
      721 cttgaaggga tgcttctggg ttttatcaat gaatatgggg cactgcttga tggtacaacg
      781 atatattttg aaaacaataa aaatcactat gaaggctgta tatttactga aaattatttt
      841 tcacatgaat tagcacgttt ttctgagata ctttcagaaa tcaatacatt caggtccaac
      901 aatccaacat ttcatgtaac ccgtgatgaa ttgatagaag gtataaataa aaagaaaaaa
      961 agcctgtacc ccatagaacg ctttatgacc gtgattggcg atatgtgtta tgatattggc
     1021 attgaattat ttaccctgta taatcgtcat cgccggtggg ttttgagtgg ttcatcgggc
     1081 aaagacgatg ctgtgatgcg cacggcattt tctgcacata catcagggtt atcatcactt
     1141 gatgacaggg aagcaatgcc tgtccccttt tccgattgta tcataaccgg tttttcaaaa
     1201 ttgcggccac tgtcaaaact gttgctacac aagccggtca tacatcccac actgcgtgat
     1261 ggagtgttcc ctcagatggt tgcttatctc ctgcagatgg cacatgtatg cctcaatgac
     1321 aggctatatg gtatactgga tgaacgtaca aaaatattat cacaaatacg gagtctttca
     1381 tgaggctttt agtaatcctt gccatgcttt cttttgcatt gcgcgctgag gcagcaccac
     1441 gtgttctcgt gttcaaatcg tatcatgaca acctcacttc attacttgat gaatatcgta
     1501 ttccgtatat tgtaaaacgg gcatcggtag tggcgggcga tagttccctg tcagggtttg
     1561 atgctgtgtt tataccctgc ggtgttgagc agcctattga ggcaagcatt caggtaatgg
     1621 cacatgggca acgtattcag ggtgttgcat taaaagatga ttatgaaggt atagatttta
     1681 agcgattgaa tgcactgctt caggattttg tgaaaagcgg tggtaggtta tatgctgcag
     1741 gttattcata cagatttgtt gaagcattag gatgttctct atcctttttt aataattttc
     1801 cccattatgg caccagcggc aggatacata gctttttaaa tgatgatttt gcttcatttg
     1861 ttatgcgtga tgacatgcaa cttactgtca actataatgg gtgggtggca ttgcaaagag
     1921 ccaatgcatc catactggcc aaagggtatt tccccactgt cggtggtgag cgggaagggc
     1981 ctgttgcttt ttttaaaaaa tatggtaatg gggttgtgta ttattcagcg taccataccg
     2041 gcgaggactc gttgtatatg cgctatttta ttttcagaac agcgtatggc atgcttgttg
     2101 atgagctttt acagaaagcg tggtactggg gtcagagcat agcattatcg gtggttgaca
     2161 cgtttaaggg tgacgaatat gtcagaagtt atacctgcac gctacctcag ggtgctatta
     2221 caatatatct tccaaccacg tacccggtac aggttgatat ttttgccaac aaaaaacttt
     2281 taggttcatg ggacagtgga tttataactg aaatatcatt gtataaccct gcaacacagg
     2341 atgctacagt atatgtatat cagagtaaca gagcagcgta taaacccttt gcactggtta
     2401 tagcacaggg taggcgtata ctgccgtata tgccagtagt gcgccttagc atgggtgcta
     2461 ttgctgtcat tatgctttta ggatttacat ggaagaggat aaatccccga aagtttgccg
     2521 gtaaacgccg ctactggttc agggatgggc attttattca taggcaaaaa taaataggta
     2581 tttatattcc cccacaacct gtggtgtgag tatatagtaa aaaaagcttg caattgaagg
     2641 tatacaattc atacatatgt atacatttaa cgataacata catatcatag cagggggctg
     2701 tcccaaaagt cgtttcgata caaatttcgc aacgctcatt gagtaatt
//
LOCUS       DAOSZP010000293         2742 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_28744_length_2742_cov_3.004466, whole genome shotgun sequence.
ACCESSION   DAOSZP010000293 DAOSZP010000000
VERSION     DAOSZP010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2742)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2742)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2742
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28744_length_2742_cov_3.004466"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..718)
                     /locus_tag="PK348_06985"
     CDS             complement(<1..718)
                     /locus_tag="PK348_06985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65995.1"
                     /translation="MKKYFILFMIVVFLNSMLYAADLGVGISAWYADWKMERKSSTVG
                     GTVNMDEVLYIGPSIAYQFSDNWSTTLVALATPQKYTWEMGTERMELRRYDVDLALNY
                     QINRYIKVFGGGKYLAFAYHNKTNGKDGIHHALGPGGGISFTMPIITNVYLLANLSGL
                     YIYGKQQEEQTTGEQSINFYEIGANGSMQLAYYIPSVAVTLAAGYRYQYVESHYTENS
                     SANPDLEHTFKGFTVLLVKSF"
     gene            complement(868..1587)
                     /locus_tag="PK348_06990"
     CDS             complement(868..1587)
                     /locus_tag="PK348_06990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65996.1"
                     /translation="MRKNKIYIMFLILFVASKIYATDLGVGISAWYADWKMESKTLGT
                     FNLEPALFVGPTISYQFEEKWSITLIALATVNKYDQDISANDSVKYRRYDVDCTLNYQ
                     ISRYIKCFAGIKYLAFDFNSDNYNGINHGVGPGAGIGFSSPIVSSIYLLGIVSGIYGY
                     GLETTDNPPAGSPDNRNFYSFGINSSLQLAYYIQAIETTISAGYRYQYIQLHYLDYGT
                     DEDSKYDNHFRGFTASVAKSF"
     gene            complement(1621..2328)
                     /locus_tag="PK348_06995"
     CDS             complement(1621..2328)
                     /locus_tag="PK348_06995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK65997.1"
                     /translation="MKLFKIIIYSILTFIISPSLYASDLGVGISAWYADWKMYNPDNP
                     NDKTNKMDPAFCIGPSVSYQFAQSWSTTLVALFTPSPYEMSNESSDATKIRRYDSDLV
                     LNYQLVRYLKIFFGGKYLGFTFKDGKHHGAGPGGGIGLTIPVIDNFYFLGNVSGLYLW
                     GSHKDNDSSKDFTEYGYNAAVQLAYYASSAGLTTSIGYRYQFIKSRYDTDDPYGKKDE
                     HTFKGFTFLIVKSFHWE"
     gene            complement(2522..>2742)
                     /locus_tag="PK348_07000"
     CDS             complement(2522..>2742)
                     /locus_tag="PK348_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003332817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HXK65998.1"
                     /translation="PKGAFYAFPDISSTGLSSQEFALKLLKSHKVAVVPGTAFGSSYD
                     NHIRCAYATSMEQLKEAMDRMEDFVKGL"
BASE COUNT          881 a          520 c          460 g          881 t
ORIGIN      
        1 caaaagattt tacaagtaat actgtaaagc ctttaaatgt atgctctaaa tctggattag
       61 ctgagctgtt ttctgtatag tgagattcaa catattgata tcggtaccct gctgcaaggg
      121 taactgcaac agaaggaata taataagcta attgcataga accattggca cctatttcat
      181 aaaaattgat actttgttct ccagttgttt gttcttcttg ctgttttcca taaatgtata
      241 atcccgaaag attggccagt aaatatacat tggttataat aggcatggta aaacttatgc
      301 cacccccagg acctaaagca tgatgtattc catctttccc atttgtttta ttgtggtatg
      361 caaaagctaa atattttccc ccgccaaaaa cttttatgta ccgattaatc tgatagttca
      421 atgctaaatc aacatcatac ctgcgcagtt ccattctttc agttcccatt tcccatgtgt
      481 atttttgtgg tgttgccagg gctaccagtg tagtactcca gttatctgaa aactgatatg
      541 caatggaagg gccaatgtac agtacttcat ccatattcac tgtgccaccc acagtgctgg
      601 attttctttc catcttccag tcagcatacc atgctgatat gccaacaccc aggtcagcag
      661 catataacat ggaattcagg aatacaacta tcatgaacag tataaaatac ttcttcatag
      721 tttataacct ccaaaaaata attttttgtt ttaatatcct tgaagggatt ctacagccat
      781 tcttatgatt gtaaaagcac atactaaata acttgtaagt attcaatatt ctatactaca
      841 taataattct tgtatattac gataaaccta gaatgattta gcaactgatg ctgtaaaccc
      901 tcgaaaatga ttatcatatt tgctatcttc atctgttcca taatccaggt aatgaagctg
      961 aatgtattga taacgatatc cagctgaaat tgttgtttcg atagcctgta tgtaatatgc
     1021 cagttgaagg gaagagttaa ttccaaatga ataaaaattt ctattgtccg ggctcccggc
     1081 aggaggattg tcagttgttt ccagcccata cccgtagata ccggatacta tgccaagaag
     1141 gtatatgctt gaaacaatag gggatgaaaa gccaatgcct gcacctggtc caactccatg
     1201 atttatgcca ttataattat cagaattaaa atcaaaagct aaatacttta ttcctgcaaa
     1261 acactttata tatcgggaaa tttgatagtt aagggtacaa tctacatcgt atcgcctgta
     1321 ttttacagaa tcatttgcag aaatatcctg atcatattta tttacggttg caagagcaat
     1381 caacgtgatg ctccattttt cttcaaactg ataagaaata gttgggccca caaataaagc
     1441 tggctcaaga ttgaaagtac ctaacgtttt gctttccatc ttccagtcgg cataccatgc
     1501 tgatatacca acacccaggt cagtagcata tatttttgat gcaacaaaca gtattaaaaa
     1561 catgatgtat attttgtttt ttctcacaat aaaaaactca aatgtaataa aatttaaaat
     1621 ctattcccag tgaaacgatt ttactataag gaacgtaaat cctttaaatg tatgctcatc
     1681 ctttttgcca taaggatcat cagtatcgta tctggatttt ataaactgat atcgatagcc
     1741 aattgaagtg gttaaacctg ctgacgaagc atagtacgct aactgtactg ctgcattata
     1801 gccatattcg gtaaaatctt ttgagctatc attatctttg tggcttcccc acagatacag
     1861 gcctgaaaca ttcccaagaa aataaaaatt gtcaataact ggaatggtta acccaatgcc
     1921 accaccgggt cctgcaccat gatgtttccc atctttaaat gtaaaaccca aatatttgcc
     1981 gccaaagaat attttcaggt atctcactaa ttgatagttt aatactaaat cagaatcata
     2041 acgtctgatt tttgttgcat cgctggattc atttgacatt tcatatggtg atggagtgaa
     2101 caatgccacc agtgtggtac tccaggactg ggcaaactga tacgaaacag aagggccaat
     2161 acaaaaagca gggtccattt tatttgtttt atcatttgga ttatctggat tgtacatctt
     2221 ccagtcagca taccatgctg atatgccaac acctaaatct gatgcataca atgatgggga
     2281 aataataaat gtcagaatac tatagataat aattttaaac aatttcatgt attacctcca
     2341 gataattata gtatacaaag tctatggtat gggataagat tatgatacat aaagatagta
     2401 taatgtatgc ctggtaacag aaatggcgaa ctctatcatt tttttagtaa aacatataaa
     2461 tgaaaatgtc aatgcatttt catattttta tgtttgtaca tttttttgtt actgtaactg
     2521 gttacaatcc cttcacaaaa tcttccatac ggtccatggc ttctttgagc tgttccatgg
     2581 atgttgcata ggcacagcgt atgtgattgt catacgagct tccaaacgca gttcctggca
     2641 caacagcaac cttatggctt tttaaaagtt taagtgcaaa ctcctgtgat gataaccctg
     2701 tggatgatat atcaggaaag gcgtaaaagg cacctttggg tg
//
LOCUS       DAOSZP010000294         2739 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_28789_length_2739_cov_2.346125, whole genome shotgun sequence.
ACCESSION   DAOSZP010000294 DAOSZP010000000
VERSION     DAOSZP010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2739
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28789_length_2739_cov_2.346125"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            133..747
                     /locus_tag="PK348_07005"
     CDS             133..747
                     /locus_tag="PK348_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010933379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdE family protein"
                     /protein_id="HXK65999.1"
                     /translation="MVNPKEYLEFGQKFHGHKCPAMPMGLRVGAAAMNALGVERAKDG
                     QLIALIELGEDHCATCFADGIQVITGCTFGKGNIKKLHHGKWGVTLIDRATGNAVRVV
                     PKAEAMMKNKNSEFMQNYRKKGIPASQVPEHVVEPLIEMVMNAPDEQILSIGKIHKQE
                     VPPKKESFEGFVCDICGEMTVEGYGRPLGEKKVCHPCYEKALHA"
     gene            847..1593
                     /locus_tag="PK348_07010"
     CDS             847..1593
                     /locus_tag="PK348_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018085295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HXK66000.1"
                     /translation="MIIPLLAGIITFLFTTVLTIAGVGAAFILIPVFIGLKIDIHVAM
                     ATALLLNSIAMTFASYRFYKDKLIMWQVALPILVIATALSPLGAWTSRFLDRTVLLWL
                     FTAFLVFAGSMMLFYQPKASQKEHSHMTQIISGLLVGAFAGFLGGLLGVGGGNFIVPV
                     LVWLGYDPKKSSATTSFIVIFSSLSGFMGHATLGAINVPLLAFTASGSATGALLGAWL
                     MSKKLQQKQVKAIIGVVLFAIAIKMAWSLL"
     gene            complement(1597..>2739)
                     /locus_tag="PK348_07015"
     CDS             complement(1597..>2739)
                     /locus_tag="PK348_07015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66001.1"
                     /translation="NDGTNGVAAVKLYGTLNIPQNITTGNFEYCIYLVEGVDMIFVDG
                     VITYPDTPRTKIFKSSIPQLARIYDPSWQQVAPCPLYVSMHASKKKPFTVLKRNYLGV
                     ESQYIIDYFKHSSKNLDLANVNNHITAEYVAITNGKECIAVAKENATLSNFAFCPLQV
                     AHSFFTGFSVSLNPFGTFFGRQYYQPTWGKGRGFEAAILNGDQYYSGACTYSGTTQRF
                     ALAIVHSNNSLPETLKRLMISYANQPFAILMHEKHEKKTTPPLQPPKGVFALYRDSGV
                     YVNFEKTDGAASYIIHCGDTKDSLSALYRTKDTSIFVKEYSKGKSFVQGNTYYVAVQA
                     VSAQGKQSELSAMYAFTAKEIKEDMDLPLTLQLKIMFDTLIAKLRW"
BASE COUNT          826 a          501 c          566 g          846 t
ORIGIN      
        1 aaaattgcgt gaactggcaa atcaggaatt atttttttag caattgattc aaatcaatgc
       61 caaaacttac ctaacagtat acattgatac acatattatt aaaatctcta tataagctta
      121 aggagttaca gtatggttaa tccaaaagaa tatcttgaat ttgggcagaa atttcatggc
      181 cacaagtgcc ctgccatgcc catgggttta cgtgttggtg cagcagcaat gaatgcttta
      241 ggagtggaac gtgccaaaga tggtcagctt atagcattga ttgaattggg cgaagatcac
      301 tgtgcaacat gttttgctga cggcatacag gttattactg gttgtacatt tgggaaggga
      361 aatattaaaa agcttcatca tggtaaatgg ggtgtaacac ttattgatag agcaacagga
      421 aatgcagttc gtgttgtccc aaaagcagaa gcaatgatga aaaataaaaa cagtgaattc
      481 atgcaaaatt acagaaaaaa gggaatacct gcatcacagg ttcctgaaca tgttgtggaa
      541 ccacttatag aaatggttat gaatgctcct gatgaacaga ttttatctat aggtaaaatt
      601 cataaacagg aagtgcctcc aaagaaagaa tcctttgaag gttttgtgtg tgatatctgt
      661 ggtgaaatga cagttgaagg atatggacgt ccattgggtg aaaaaaaagt ttgtcatccc
      721 tgctatgaaa aagcattgca tgcataataa gttgctttaa taaaacggaa ttggtagtac
      781 cagcattacg gtataataat gccatgctgg tgctaccatt aaaaaatttc atggaggatt
      841 actaaaatga taataccatt attagccggc ataataacat ttttatttac cactgtttta
      901 actatagcgg gcgttggtgc agcttttatt ttaattcctg tatttattgg actaaaaata
      961 gatattcatg tagccatggc aactgcatta ctcctcaatt ccatagccat gacctttgca
     1021 tcatatcgat tttataaaga taagctgata atgtggcagg ttgcattacc aatccttgta
     1081 atagcaactg cactatcacc actgggtgca tggaccagca ggtttttaga ccgaactgtg
     1141 cttttgtggc tttttacagc atttcttgta tttgcaggta gcatgatgct tttttatcag
     1201 ccaaaagctt cacaaaaaga acattcacat atgacacaga ttatatcggg tctgttagtt
     1261 ggagcctttg caggctttct gggaggattg cttggagttg gtggtggaaa ttttattgtc
     1321 cctgtactgg tgtggttagg atatgaccca aaaaaatctt cagcaaccac atcgttcata
     1381 gttatttttt cgtcactttc gggttttatg gggcatgcca cattaggtgc aattaatgta
     1441 ccactcctgg catttactgc ctcaggatca gcaacaggag cacttttggg agcatggctc
     1501 atgagtaaaa agctacaaca aaagcaggtg aaagctatca taggcgttgt attatttgcc
     1561 attgccataa aaatggcgtg gagccttttg taaacttcac cagcgcagtt ttgcaatgag
     1621 tgtatcaaac ataattttta actgcaacgt tagaggcaaa tccatgtctt cctttatttc
     1681 tttagcggta aaggcataca tggccgaaag ctcagattgt tttccctgtg cgctcacagc
     1741 ctgtaccgca acatagtatg tattgccctg aacaaacgat ttgccctttg aatattcttt
     1801 tacaaatatc gatgtatctt ttgttctata cagtgcagac aatgaatctt ttgtatcacc
     1861 acaatgaatg atgtaggaag cagcaccatc agttttttca aaatttacat atacaccgct
     1921 atcgcggtat aatgcaaaca ctccttttgg cggctgtaat ggtggtgtag tttttttctc
     1981 atgcttttca tgcatgagta ttgcaaatgg ttgatttgcg tatgaaatca tcagtctttt
     2041 taatgtttca ggcaatgagt tattactatg aactatcgcc aatgcaaaac gctgagtggt
     2101 tccgctgtaa gtgcatgcac ctgagtaata ctgatcacca tttaagattg cggcttcaaa
     2161 ccccctgccc ttaccccatg ttggctgata gtactgcctg ccaaaaaatg ttccaaacgg
     2221 attgagactt actgaaaatc ctgtaaaaaa actgtgcgcc acctgtaatg gacaaaaagc
     2281 aaagttggaa agagtagcat tttcctttgc aacagcaata cattccttcc cattggtaat
     2341 tgccacatac tctgcagtga tgtgattgtt gacattggca agatcaagat tttttgatga
     2401 atgcttgaag taatcaatta tgtactgaga ttctacaccc aggtaattgc gctttaatac
     2461 agtaaaaggc tttttcttgg atgcatgcat tgacacatac aacgggcatg gagcaacctg
     2521 ttgccagctg ggatcataga tacgtgcaag ctgcggtatg cttgatttaa aaatttttgt
     2581 gcgcggtgtg tcaggatagg tgattactcc atcaacaaaa atcatgtcaa caccttctac
     2641 cagatagatg cagtattcaa aattacctgt ggtgatattc tgtggaatgt tgagggtacc
     2701 atacagtttt acggctgcaa caccattggt gccatcatt
//
LOCUS       DAOSZP010000295         2738 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_28805_length_2738_cov_3.817741, whole genome shotgun sequence.
ACCESSION   DAOSZP010000295 DAOSZP010000000
VERSION     DAOSZP010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2738
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28805_length_2738_cov_3.817741"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            212..1888
                     /locus_tag="PK348_07020"
     CDS             212..1888
                     /locus_tag="PK348_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010527513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HXK66002.1"
                     /translation="MELPQATNFIEEIINEDLKNNVCSSVITRFPPEPNGYLHIGHAK
                     SICLNFGIAQKYNGKCNLRLDDTNPEKEDIEYVESIKKDIQWLGFQWDDDVKHASDYF
                     EQLYEWAKVLIKNGKAYVCDMSAEEVAATRGTPTEPGKESPYRNRSVEENLDLFERMK
                     NGEFPDGSKTLRAKMDMTSPNMHMRDPVMYRIKHADHHRTGDRWCIYPTYDWAHGQSD
                     YIEGITHSICTLEFEVHRPLYDWFLDQIATGQRPKQIEFARLNLTYTVMSKRMLLRLV
                     QEGFVNGWDDPRMPTIAGLRRRGFTPDSIKNFAKLVGVAKKDSIVDIALLEHCIREDL
                     NKKANRVMCVLNPIKVVILNYPDNQVEYIEGINNPEDPAAGTRNIPFSRVLYIERDDF
                     MENPPKKFFRLSPGAEVRLRYTYFIKCVNVIKDSNGNITEIHCTYDPDTKDGYAQDGR
                     KVKGTIHWVSAQHALPAEIRLYDRLFTKENPSEVEEGRDFTDYINPDSLKVVNGYIEP
                     YVQGAQVYTNFQFERLGYFTVDPDSNNDRLIFNRTVTLKDTWAKIAQKED"
     gene            1958..2030
                     /locus_tag="PK348_07025"
     tRNA            1958..2030
                     /locus_tag="PK348_07025"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1991..1993,aa:Gln,seq:ctg)
     gene            2113..>2738
                     /locus_tag="PK348_07030"
     CDS             2113..>2738
                     /locus_tag="PK348_07030"
                     /inference="COORDINATES: protein motif:HMM:NF015557.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HXK66003.1"
                     /translation="MYSIALIIFIITYSGIIFTRLPWVNVDRPSAAYFGAIAMILTGV
                     LSFEEAILAIDFNTIALLLGMMIVIATLELDGFFAFIAQKTISFSRTPLQLLWIITFT
                     TGIASAFLVNDAVVLMFTPIIISICRSAQLDPKPYLIAEILASNVGSAMTITGNPQNM
                     LIGIYSGIPYVYFLLHLLPVSIIGMIIIVVCIKRMYSHTFETQQVLQY"
BASE COUNT          879 a          498 c          536 g          825 t
ORIGIN      
        1 tgcgtgaatt gaaaaattat gtataaagaa gagatcccag agaaattttg ataaggtaag
       61 aataaaaaat cagagtctta aaattgacag aaaaaagggt cagagcctta ttacagtaac
      121 tatcgcatat ataaaagatt tataatgtct tgacaaaata cacttctttc ttcctacagt
      181 atgtaacaaa ctaatacaaa gcggtactgc catggaacta ccacaagcta cgaattttat
      241 tgaagaaatt atcaacgaag acttaaaaaa taatgtttgt tcatctgtta ttacgcgctt
      301 ccctccagag cctaacggct atcttcatat tggacatgca aaatcaatat gccttaattt
      361 tggtattgct caaaaataca atggcaaatg caatttgcgt ctggatgaca ccaaccctga
      421 aaaggaagat attgagtatg ttgaatcaat taagaaggac atccaatggt taggatttca
      481 gtgggatgat gatgttaaac atgcttccga ttattttgaa caactgtatg aatgggctaa
      541 agttttgatt aaaaatggta aagcctatgt ttgtgacatg agcgctgagg aagttgcagc
      601 aacgcgtggt acacctaccg aacctggaaa agaaagccca taccgcaacc gcagtgttga
      661 agaaaattta gacttatttg aacgcatgaa aaacggtgag tttcccgatg gttcaaaaac
      721 attgcgtgca aagatggaca tgacatcgcc aaatatgcat atgcgtgatc ctgtgatgta
      781 tcgcataaaa catgcggacc atcatcgtac gggcgataga tggtgcattt atcctaccta
      841 tgattgggct catggtcaat ccgactacat tgaaggaata acgcattcca tttgtactct
      901 ggaatttgag gtacaccgtc cattgtatga ctggttttta gaccagatag cgacaggcca
      961 acgtccaaaa cagattgaat ttgcacgtct taacttaacc tataccgtca tgagcaaacg
     1021 tatgctttta agactcgtgc aggaaggctt tgtaaatgga tgggatgacc cacgcatgcc
     1081 taccattgca gggctcagaa gaagaggttt cacccctgat tccattaaaa attttgcaaa
     1141 gcttgttggt gttgcaaaaa aggatagtat tgttgatatt gcactgcttg aacattgcat
     1201 acgggaagat ttaaacaaaa aagccaaccg tgttatgtgt gtgctcaatc caattaaagt
     1261 ggttatactt aactatcctg ataatcaggt tgaatacatt gaaggtataa ataatcctga
     1321 agaccctgcc gctggtaccc gaaatatacc attttccaga gtcctttaca ttgaacgtga
     1381 tgattttatg gaaaatccgc caaagaaatt tttcaggctt tcacctggtg ctgaagtgcg
     1441 tttgcgatat acgtatttta ttaagtgtgt aaacgttata aaagatagca acgggaacat
     1501 tactgaaatt cattgcacct atgaccctga cacaaaggac ggatatgcac aagacggtag
     1561 aaaagtcaaa ggcaccattc actgggttag tgcacaacat gcattacctg ctgaaataag
     1621 gctttatgac agattgttca caaaggaaaa tccgtctgaa gttgaagaag gcagagattt
     1681 tactgattac attaatcctg attcattaaa agttgtaaat gggtatatag aaccctatgt
     1741 acaaggtgca caggtttaca ctaatttcca gtttgaaagg ttgggctatt ttaccgttga
     1801 tcctgattct aacaatgaca gactcatttt taatagaact gtaacactga aggatacatg
     1861 ggcaaaaata gctcaaaaag aagattgaat acagtcaaaa ttattgacaa aatttttgtt
     1921 tacagaaact atgtgctata gattgtttat atgaaattgc ccggtggtgt aattggcaac
     1981 acatctgact ctggatcaga gaagtccagg ttcgagtcct ggccgggcag catttatgta
     2041 ataaaaatgc agaggaatcc tacaaatcct ctgcattttt aatttaaggt ttctttatca
     2101 gtaactatcg ccatgtattc tattgcattg ataatattta taataacgta ttcaggcata
     2161 atttttactc gccttccatg ggttaatgtt gacaggccat ctgctgctta ttttggcgct
     2221 attgccatga ttttaacggg agtgctttct tttgaagagg ctattcttgc tattgatttt
     2281 aacacaatag ctcttttact gggcatgatg atagtcatag caacattaga actggatggt
     2341 ttttttgcct ttattgcaca aaaaactata tctttttcca gaaccccgtt acagcttttg
     2401 tggatcatta ctttcactac tggtattgca agcgcatttc tggttaatga tgcagttgtc
     2461 ctgatgttta cccccatcat aatatcaata tgcagatcag cgcaacttga ccccaaacca
     2521 tatctgatag ccgaaatatt ggcttctaat gtgggcagtg caatgaccat cacaggtaat
     2581 ccacagaaca tgctcattgg catttattca ggaattccct atgtatattt tcttcttcac
     2641 ctgcttccag tttcaattat tgggatgata attattgtag tatgcatcaa acgtatgtat
     2701 tcacacacat ttgaaacaca gcaggtattg caatatag
//
LOCUS       DAOSZP010000296         2738 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_28812_length_2738_cov_2.678718, whole genome shotgun sequence.
ACCESSION   DAOSZP010000296 DAOSZP010000000
VERSION     DAOSZP010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2738
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28812_length_2738_cov_2.678718"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            232..684
                     /locus_tag="PK348_07035"
     CDS             232..684
                     /locus_tag="PK348_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008837042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HXK66004.1"
                     /translation="MKWGLTKRNETKLNPFEAFERDVKRIFDDFFTLEPVDLFDSAWV
                     PSIDVEEDEKSIHVRAEIPGIDEKDLNVTLEDNVLTISGEKKEERKEENKRYVLAERR
                     FGSFKRSIALPAEVKADNVKASFKNGVLTVDFEKKEVSQPKRITINVK"
     gene            698..1108
                     /locus_tag="PK348_07040"
     CDS             698..1108
                     /locus_tag="PK348_07040"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HXK66005.1"
                     /translation="MFTTYDLLDDVLTLRDMVDRFFDEVSTTRRRVEFPYINVYEKDD
                     DIVIRAIAPGVSSSDVNVQLADNTLTIEMNRKEDYADKPYLRKEREFGSYKKSIKLPY
                     YVDPDRVEATMNNGILTIKLTKHESVKPRKITIN"
     gene            1124..1504
                     /locus_tag="PK348_07045"
     CDS             1124..1504
                     /locus_tag="PK348_07045"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HXK66006.1"
                     /translation="MATQVAKRTDRNLDKKDYCLVPPVDIYETDNEYVLKADMPGVPK
                     ENIEITFNNNELEINGKIDEAYTSSDNLTYREFTLCNYNRKFLVSDKINVEGITATLE
                     NGVLTVTLPKREEAKPKKIEIKAE"
     gene            complement(1543..1953)
                     /locus_tag="PK348_07050"
     CDS             complement(1543..1953)
                     /locus_tag="PK348_07050"
                     /inference="COORDINATES: protein motif:HMM:NF020950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease inhibitor I42 family protein"
                     /protein_id="HXK66007.1"
                     /translation="MKSYILRILIVCMVLSTGIGCSKLMNNTCTTVDAQKYAGQEIVI
                     KNDGCITIEIESQLSTGYRWRFAIKENPGMLINSDYKVQTCEGQTVGGIDKEIFTFKA
                     VKAGKATVVFDYVRPFDKNPKPVKTKEFTITITE"
     gene            complement(1974..>2738)
                     /locus_tag="PK348_07055"
     CDS             complement(1974..>2738)
                     /locus_tag="PK348_07055"
                     /inference="COORDINATES: protein motif:HMM:NF012340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HXK66008.1"
                     /translation="YSVVEDQKEEKPEAVTPAKPEETLPESFMLADPSQPVLDWFALG
                     MMTAVKDQGLCGSCWSFTAMGVVEGCYKINKKTDINLSEQYMIDCARDKYGRDAGSCN
                     GGWYGNVFDFMMTNYPVEEKIAPYRGYEYGCTPKKQIPIKLVAWGYVRKDGGIPSVDE
                     MKKALCTYGPIAATVKVTPAFQAYVGGIFDEFTPVSSPKDVNHGIIIVGWDDSKKAYR
                     IKNSWGERWGEKGYMWIEYGCNNIGYGAAWVVLDKE"
BASE COUNT          861 a          566 c          479 g          832 t
ORIGIN      
        1 atttattttt aaacaatgtc atgatgttat cccccttgtt ttcctgtaaa tagaaaacgc
       61 tggaaatctt aaaaatttaa aaaaatttca caattttcag tgtctttcta aaaatttttc
      121 tcctgcccca gttgacgatt ttaaaatctg ttattaattt attattagaa attagcactc
      181 attagtatag agtgctaaca ataacaacct tatattcata ggaggtgcac tatgaaatgg
      241 gggttaacaa aacggaatga aaccaaatta aatccttttg aagcttttga acgcgatgta
      301 aaaaggattt ttgatgattt ctttacactg gaaccggtag atctttttga ttctgcatgg
      361 gtaccttcaa ttgatgttga agaagatgaa aaatctattc atgtacgggc tgaaatcccc
      421 ggtattgatg aaaaggacct caatgttact cttgaagata atgtactgac catcagtggc
      481 gaaaagaaag aagaacgcaa agaagaaaac aaacggtatg tacttgcaga acgccgattt
      541 ggttcattca aacgtagcat tgcattgcct gctgaagtaa aggctgataa tgttaaagcc
      601 tccttcaaaa atggtgtttt gactgttgac tttgaaaaga aggaagtttc acagcccaaa
      661 cgaataacta tcaacgtaaa ataacaggag gtggactatg ttcacaacat atgatcttct
      721 tgatgacgtt ttaacactca gggacatggt ggaccgcttc tttgatgaag tatccaccac
      781 ccgtcgacgc gtggaattcc cttatatcaa tgtctatgaa aaagatgatg atatcgttat
      841 acgggcgata gctccgggag tttcatccag cgatgtcaat gtgcaactgg ctgataatac
      901 attaactatc gaaatgaacc gtaaggaaga ttatgctgac aaaccgtatt tgcgcaaaga
      961 acgtgagttt ggcagctaca agaaatccat caagttgcca tactatgtgg accccgacag
     1021 ggttgaagca acaatgaaca atggtatttt aaccattaag ctgaccaaac atgagtcagt
     1081 gaaaccccgc aagataacca ttaactaaaa ggaggtgata gttatggcaa cacaggtagc
     1141 aaaacgcact gatagaaatc ttgataagaa ggattattgc ctggtacctc cggttgatat
     1201 ctatgaaacc gacaatgaat atgtcctcaa agcagatatg cccggagttc ctaaggaaaa
     1261 tattgaaata accttcaata acaatgaact ggaaatcaac ggtaagattg atgaagctta
     1321 tacctcaagt gacaatctga cataccgtga atttacactg tgcaattaca accgtaaatt
     1381 cctggtttct gacaagatta atgttgaagg cattactgct acacttgaaa acggtgtact
     1441 cactgttaca cttcccaaac gtgaagaagc aaaaccaaag aagatagaaa taaaagctga
     1501 ataatattat gccccatcgt taacagaaga tggggcattt ttttattctg taattgttat
     1561 agtgaattct tttgttttta caggttttgg attcttatca aacggacgca cataatcaaa
     1621 tacaacagta gctttgccgg ccttaactgc cttaaatgta aatatttctt tatctattcc
     1681 accaacggtt tgcccctcac atgtctgcac tttataatca ctgttaataa gcattccagg
     1741 attttcctta atagcaaagc gccatcggta tcctgtactc aattgtgatt caatttcaat
     1801 tgtgatacag ccatcgtttt taataacaat ttcctgtcct gcatacttct gtgcatctac
     1861 cgttgtacat gtattattca taagttttga acaacctatt cctgttgata gtaccataca
     1921 tactatcagg attcttaata tatatgattt cataaaacct ccatgaattt tcattattct
     1981 ttgtcaagga caacccacgc tgccccataa ccaatgttat tgcatccata ttctatccac
     2041 atgtagcctt tttcgcccca acgctcaccc caggagttct ttatcctgta agctttttta
     2101 ctatcatccc aacctacaat aattattcca tgattgacat ctttggggct gctcaccggt
     2161 gtaaattcat caaatatacc accaacatat gcctgaaaag ccggggtaac ctttacagtg
     2221 gcagcaattg gtccataggt acataatgcc tttttcattt catcaactga agggatacct
     2281 ccatctttgc ggacataccc ccatgccaca agctttatgg gtatctgttt tttaggagtg
     2341 catccatatt catatccccg atatggggca atcttttctt caacagggta gttggtcatc
     2401 ataaaatcaa atacattgcc ataccatcca ccattacaac tgcctgcatc ccgtccatat
     2461 ttatcacggg cacaatcaat catatattgt tctgaaagat taatatcggt ttttttattt
     2521 atcttataac aaccttccac cacacccatt gcggtaaatg accagcagct tccacacagt
     2581 ccctgatctt taacagccgt catcattccc agcgcaaacc agtcaagcac tggctgtgaa
     2641 ggatcagcta gcatgaatga ttcgggcaat gtttcttctg gttttgctgg ggtaactgct
     2701 tcaggctttt cttctttttg atcttcaaca acagagta
//
LOCUS       DAOSZP010000297         2731 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_28887_length_2731_cov_3.229821, whole genome shotgun sequence.
ACCESSION   DAOSZP010000297 DAOSZP010000000
VERSION     DAOSZP010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2731
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28887_length_2731_cov_3.229821"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2172)
                     /gene="uvrA"
                     /locus_tag="PK348_07060"
     CDS             complement(<1..2172)
                     /gene="uvrA"
                     /locus_tag="PK348_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HXK66009.1"
                     /translation="MSQQYIRIKGAREHNLKNISIDIPREKLVVITGLSGSGKSSLAF
                     DTIYAEGQRRYVESLSAYARQFLGQMEKPDVDLIEGLSPAISIEQKTTHRNPRSTVGT
                     VTEIHDYLRLLFARVGVPYCYKCGKPITSQTIDQIVENVLTHTAGTKIQVLAPVVRGR
                     KGMYQDMLEKFKQQGYSRVRVDGQVYSLDDTIKMDKNKKHSIEVIIDRIVIKENIRSR
                     LSDSIETAIELADGQVILIIDDNEQLISTKMTCPDCDVSLPELSPRMFSFNSPYGACP
                     TCDGLGFIMQFDPELIVANPDKSLMDGALAVWGKTTSYWYQEQVLTLARYLKFDPHTP
                     WKKLPKQIQNIVLYGSNGKKFDYSIERFEAQYRFTKEFEGVIPNLMRRYKETQSDEMR
                     EWMEGFMRNRVCDACGGKRLKPESLAVKVADKNIADVSAMSIKEAVKFFKDISLSETQ
                     MTIARQILKEIRARLQFLSNVGIDYLTLDRAAGTLSGGEAQRIRLATQIGSGLTGVLY
                     ILDEPSIGLHQRDNKKLLDTLKELRDIGNTVIVVEHDEETIRESDYVIDLGPGAGLDG
                     GYVVAHGTPQEIEQNETSITGLYLSGKRYIKAPLKRRVAEKYLTIRGAAENNLKSIDV
                     SFPLGVFTCVTGVSGSGKSTLIIDILYKALASLKMRSKDYPGKFKKIEGIEYIDKVID
                     VDQSPIGRTPRSNPATYTGTFTPIRELFASLPESKIRGYKPG"
     gene            complement(2169..2453)
                     /locus_tag="PK348_07065"
     CDS             complement(2169..2453)
                     /locus_tag="PK348_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66010.1"
                     /translation="MPNDFEKEFDDEVYEDDFYEETFDDEEILEEEMLEDDLIEEDEV
                     EEDEKQIAVILACDDCDYRWEDVIVKHKDEDIEDLEIICPMCGSTNITQI"
BASE COUNT          805 a          599 c          441 g          886 t
ORIGIN      
        1 cccaggctta tacccgcgaa tttttgattc aggtaagctt gcaaaaagct cacgaattgg
       61 cgtaaatgtt ccagtatatg tagcaggatt ggatcgtggt gtcctcccta ttggtgactg
      121 atcaacatca attactttat caatatattc tatcccttct atctttttaa atttaccggg
      181 ataatcttta ctgcgcattt tcaatgaagc cagcgcctta tacaaaatat caattattaa
      241 ggtagatttt cctgaaccgg aaactccagt aacacaggta aacacaccta aaggaaatga
      301 cacatctatt gattttaaat tattttccgc cgcacctcga atggttaaat acttttcagc
      361 cactctgcgc tttaacggtg ctttgatata ccgtttccca cttaaatata atccagtaat
      421 tgatgtttca ttttgttcta tctcctgcgg tgtgccgtgc gctaccacat aaccaccatc
      481 aagccctgca ccaggaccta aatcaatgac ataatctgat tcgcgaattg tctcttcatc
      541 atgctctaca acaattaccg tgtttccaat atcccgcaat tcttttaacg tatctaaaag
      601 ctttttgttg tcacgttgat gaaggccaat acttggctca tcaagaatat acagtacacc
      661 cgttaaacct gacccaatct gagtggcaag ccgtatgcgc tgtgcctcac caccagacaa
      721 tgtaccagct gcacggtcca atgtaagata atcaatgcca acattggaaa gaaattgcaa
      781 tcgtgctcgt atttctttaa gtatctggcg ggctattgtc atctgagttt ctgacaggga
      841 aatgtcttta aaaaatttta ctgcttcttt aatagacatt gcacttacat cggcaatatt
      901 tttatcagca acttttactg ctaaactctc gggctttagc cttttacctc cacacgcatc
      961 acacacacga ttgcgcatga agccttccat ccactcgcgc atctcatcag attgtgtttc
     1021 tttgtagcga cgcattaaat ttgggataac accttcaaat tcctttgtaa aacgatattg
     1081 ggcttcaaac cgttcaatgg aataatcaaa ctttttacca tttgagccat acaacacaat
     1141 gttctgaatt tgctttggta gcttcttcca tggcgtatgg ggatcaaact taagataccg
     1201 tgccagtgtt agcacctgct cctgatacca gtagcttgtt gtttttcccc acacagcaag
     1261 tgctccgtcc attaatgatt tatcaggatt agcaacaata agctctgggt caaattgcat
     1321 gataaaaccc aatccgtcac aggtgggaca tgcaccgtag gggctattga aagaaaacat
     1381 gcgtggtgat agttccggca atgaaacatc acagtcagga caggtcattt ttgtggatat
     1441 taactgttcg ttatcatcta tgattaatat gacctgacca tcagctaatt caattgctgt
     1501 ttcaattgaa tctgataatc gtgaacgtat attttctttg ataactatcc tgtcaataat
     1561 gacctcaata gaatgctttt tatttttatc catttttatg gtatcatcaa gcgaatacac
     1621 ctggccatca actctaacac gtgagtatcc ctgctgtttg aatttttcta acatatcctg
     1681 atacatacct ttgcgaccac gcacaactgg tgcaagtacc tgtatttttg tcccggcagt
     1741 atgagtaagt acgttttcaa caatctggtc aatggtttgt gaggttattg gtttcccgca
     1801 cttataacag tatggcactc caacacgtgc aaagagcaaa cgaaggtaat cgtgaatttc
     1861 ggttacagta cctactgttg aacggggatt acggtgagtg gttttctgtt caatggatat
     1921 tgcaggtgaa agcccttcaa tgaggtcaac atcaggtttt tccatttgac ctaaaaactg
     1981 cctggcatac gccgacaatg actctacata ccgccgttga ccttcggcat aaatggtatc
     2041 aaaagccaga gaggacttcc ctgaaccgga aagccctgta ataacaacca acttttccct
     2101 tggaatgtca atagaaatgt tttttaaatt gtgctcacgg gcacctttaa tgcgaatata
     2161 ttgctgactc atatctgtgt aatgttagtc gaaccgcaca taggacagat aatctccaga
     2221 tcttcaatat cctcatcttt atgttttact attacatctt cccatcggta atcacaatca
     2281 tcgcaggcta aaatcacagc tatctgtttt tcatcttctt ccacttcatc ttcttctatt
     2341 aaatcatctt cgagcatctc ttcttcaagt atctcttcat catcaaatgt ttcttcatag
     2401 aaatcatctt catatacttc atcatcaaat tctttttcaa aatcatttgg cataatctac
     2461 accctcttat actgatatta ttatgcaaaa tactactatc aatatatgaa tactttaaaa
     2521 actatctaac aaaattttaa aaaaaacata aaattttaag gcatttcctg attacacagt
     2581 ttttacacta caatacaaat gtagtgcaca actcatgcaa aaatttttaa aaaattgcca
     2641 tacctttttg tcaaaaaagt tagtatatga aaaatggtca ataaaaaaag ttattttttt
     2701 atagattttg gttttactaa ctttgcaggt g
//
LOCUS       DAOSZP010000298         2727 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_28938_length_2727_cov_3.215569, whole genome shotgun sequence.
ACCESSION   DAOSZP010000298 DAOSZP010000000
VERSION     DAOSZP010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2727
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28938_length_2727_cov_3.215569"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(262..2619)
                     /locus_tag="PK348_07070"
     CDS             complement(262..2619)
                     /locus_tag="PK348_07070"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: protein motif:HMM:NF000942.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HXK66011.1"
                     /translation="MQHTADNAIMKAVTVIGAGSWGTAVANVIAHNHPEIVVRMWAYE
                     KDVARSVNSQHINTLYLPDVQLANNIVAYTDLKESVRGTDAVIVATPSKVVYDTCTRI
                     RRYLDSKVYVGYLSKGFCKVGSGIYTMSQTIELALPFLMDKVCALYGPSHAEEVCREY
                     HTALHVASKSKSSINFFMNLLQNEYLECIPSDDIIGVDCGSTLKNPAAIAAGIISNLP
                     QCGDNLAGALIAQAHAEMIQLGRCLGAKEQTIMGIAGLGDLVATALSQHSRNRRFGRE
                     IAEQITQKGTTVSFFDKLLLRVKPENVLERMSKRMHYLVEGAYAIEPILELADKYNLT
                     MPVYRSLYDVLLNKRDPWLLIETIKNPAKYAILTRRARIKVKERKKGIERMSGMIFKN
                     IVVEQLVQQLCSESKKLQVLANSREYKELLKQYLPQHKEYSHELSLYNDLNEAQYEKQ
                     LKTIIEFYYNSISDRYVYTFSLLILKLARMFFYLYGLLYRRRITEFFEERIGLSGDIK
                     YLKKTVMTANPVYFCNAKDQADSLFVVLALIKFISIPLPRFYVDSRLMKNKLLQFLFR
                     LCGGYIVHTTRCASILYRETLLQYMLSCVEHGIPVLYSNSMDNESQDELVIQDMVIEG
                     LCALLQKTTEEIAVIPVGIGHKYYNPVTHPVSFLKLFRNVTKVHISRPITLSHFSASP
                     TLAEDTKMMLKVKQRHDIPIYPHYFVAYVLHVSGGSAHVEAIKAEIDSILKLRNLKHL
                     YSVEDIMREGVDFLISNNYIARSGETVVVLDDKQEAITYFAGYIV"
BASE COUNT          822 a          577 c          468 g          860 t
ORIGIN      
        1 ttttaaaatt ttagttacca tagttaatac atccagggaa taatatcacc ttaacggagt
       61 ttaatggtaa ggtactatgt tcttctacaa tactgttaca cctaactgtg tttatgtaca
      121 tgatacaaat ttttccaatc atgccactta tgaccgtttt taattgaatg ctactatatt
      181 gctttataat acttcaggac tattatgtga ataaattatc ttaaaacgta tatgctttaa
      241 aaacaagatt tgtaaaatac attacacaat atacccggca aaatatgtaa ttgcttcttg
      301 tttatcatcc aaaacaacta ctgtttcccc actgcgtgca atataattat ttgaaataag
      361 gaaatccaca ccttcacgca ttatatcttc aacagaatat agatgtttta aattacgtag
      421 tttcagtata ctatcaattt cagctttaat ggcttcaaca tgtgcacttc ccccactaac
      481 atgcagtaca tacgccacaa aataatgagg ataaatgggt atatcatgac gttgtttcac
      541 ctttaacatc attttagtat cttcggccaa tgtggggctg gcagaaaaat gcgaaagggt
      601 aataggccgg gaaatatgta cctttgttac attcctgaat aatttcagga atgataccgg
      661 atgagttacc gggttataat atttatgacc aattccaaca ggtattaccg caatttcctc
      721 tgttgttttc tgcaacagtg cacacaggcc ttcaatgacc atatcctgaa ttaccagctc
      781 atcctgtgat tcattatcca tggaattaga atataatacc ggtatgccat gttcaacaca
      841 tgataacata tactgcaaca gtgtttccct gtatagaatg gaagcacagc gtgtagtatg
      901 gacaatatat cccccacaaa gcctgaataa aaattgcaac aatttatttt tcataagcct
      961 gctatccaca taaaatcgcg gcagggggat ggatataaat ttaataagtg caagaaccac
     1021 aaacagagaa tctgcctggt ctttagcatt gcaaaaatac accggatttg cagtcatcac
     1081 agtctttttt aaatacttaa tgtcaccgct cagccctatt ctttcttcaa agaactctgt
     1141 aattcttcgg cgatacagca acccataaag ataaaaaaac attcttgcca gtttcagtat
     1201 cagcaaagaa aacgtataca catatctatc gctaatacta ttataataaa actcaataat
     1261 tgttttcagt tgtttttcat actgggcttc attaagatca ttataaagcg atagctcatg
     1321 ggaatactct ttatgctggg gcaggtattg ctttagcaat tctttgtatt ccctgctatt
     1381 tgcaagcacc tgaagttttt tactttcact gcataattgc tggacaagct gctcaaccac
     1441 aatattttta aatatcatac cactcatgcg ctcaatgcct tttttgcgct ccttaacctt
     1501 tatccgtgcg cggcgcgtca gtattgcata ttttgcagga ttttttattg tttcaatgag
     1561 aagccatggg tcgcgtttat tcagaagcac atcatacagg gagcggtaga caggcatggt
     1621 aaggttatat ttatcagcca gttccagaat tggctcaatt gcatacgccc cctcaaccag
     1681 gtaatgcatg cgtttactca ttctttccag aacattttca ggttttactc gtaataacaa
     1741 cttgtcaaag aaacttactg ttgttccttt ctgggttatt tgctctgcta tttctctgcc
     1801 aaagcgcctg ttacggctat gctgacttaa cgctgttgca acaaggtcac ccaaaccagc
     1861 tatacccatg atagtttgtt cctttgcacc caggcagcgt cccagttgta tcatctctgc
     1921 atgagcctgt gctattaatg ctcctgcaag gttatcccca cactgaggga ggttacttat
     1981 aatgcctgct gcaattgctg caggattttt caaggtactt ccacaatcaa caccaataat
     2041 atcatctgaa ggtatgcatt ccagatattc attctgtagc agattcatga aaaaattaat
     2101 tgaagacttt gatttgctgg ctacatgcaa tgccgtatga tattctctgc acacctcttc
     2161 agcatggctt gggccgtata gagcacacac cttatccatt aaaaaaggta gtgcaagctc
     2221 tattgtctgt gacatggtat aaattcctga accaactttg caaaaccctt tagacaaata
     2281 gccaacatat acctttgaat caagatagcg cctgattcgt gtacaggtat catacacaac
     2341 ttttgaaggg gtagcaacta ttactgcatc agttcctctg accgactcct ttaagtcagt
     2401 atatgcaaca atattgtttg ccagttgcac atcaggcagg tatagtgtat tgatatgctg
     2461 agagtttaca gaacgggcaa catccttttc ataggcccac attcgcacaa caatttcagg
     2521 atggttatgg gctattacat tggcaacagc ggtaccccat gaacctgcac caatgacggt
     2581 tacagctttc attatggcat tatctgcggt gtgttgcata gtatacccgg tattaacaga
     2641 ttaaaataac aaaaagggga tgtcccaaaa gccgtttcga tacaaatttc acaacgctca
     2701 ttgagtaatt ttgccagatt ttgtggc
//
LOCUS       DAOSZP010000299         2716 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_29083_length_2716_cov_3.230740, whole genome shotgun sequence.
ACCESSION   DAOSZP010000299 DAOSZP010000000
VERSION     DAOSZP010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2716
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29083_length_2716_cov_3.230740"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..590)
                     /locus_tag="PK348_07075"
     CDS             complement(<1..590)
                     /locus_tag="PK348_07075"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic peptidoglycan transglycosylase"
                     /protein_id="HXK66012.1"
                     /translation="MKRYNWKHIDCTSANVLKASLVVFVVAIVTYIIFPYKDGYLTAN
                     NKSPITIYDRHGLVLMEITDGKKGLSQSVKIQEVPSEFIDLLLFSEDRNFYDHWGISL
                     KGIARALYQNLQVMHIVSGGSTITQQLVKSKKEIVRNTVVSKLAEIIEAIRLECHFSK
                     KEILQAYINEVYMGNNIYGFEKAAQVYFDKHLYQCDL"
     gene            complement(593..>945)
                     /locus_tag="PK348_07080"
     CDS             complement(593..>945)
                     /locus_tag="PK348_07080"
                     /inference="COORDINATES: protein motif:HMM:NF036531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66013.1"
                     /translation="IVEITVYTPKDRGITVLEDQLPSGFEPVNVTFATEEKVAVDKKS
                     DYTNWGTFNHYEQYSDRIIYYADFIQRGTHKVIFKVRATNSGTFKLPACKVEEMYNPE
                     VFGILYFSNTVTVE"
     assembly_gap    946..955
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<956..>2716)
                     /locus_tag="PK348_07085"
     CDS             complement(<956..>2716)
                     /locus_tag="PK348_07085"
                     /inference="COORDINATES: protein motif:HMM:NF012433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HXK66014.1"
                     /translation="RFSAYYKATVVVKQGKAKVQCKLPDNLTSFKSMVVVHTEDSKFG
                     YGEATFEVKKPLMVLPTFPRFVRVGDTFNAGALVFNYTGKQGNVTIGLELKNGLKLKD
                     GSAVAVKELILNNGQSQEVTFPVTVTGQAGMAGFTVKAQAGTYTDGITDTIPIKAPNV
                     FETVAQYGRISAPQSREVQEITITRNIIPEVSSINIFTSPSAFVDLKGCLDYLVDYPY
                     GCFEQKASKILPLILGQDIIVQKGLLKTKSKDELRKVVTDVLKEFPAYYKNGGFSYWK
                     DGSLPSSYLTVYAAFIMTMAKKRGYDVDNAIYNDTIKVIKQFVKKGNIFSHEGRNSSW
                     YTYTTLAFAHYVAALQGFISENELKLVYSTITKKYDDCIAAYAYLLKALALYPDSTLK
                     KTLVKEIAEKFFSRMRTESGYVYFTDTSQWGYFYYDTVLSTALILQALLEANIPFNND
                     YKIVQYLLKQRKGYAWLNTHQNAVVWWALNSYLVRYEKDKPDYSLQVLVDTKELLNAI
                     VNESNVHFAKQYIVTSEDSGVKKFSFIKNGKGACYFTMQYQYIPVYEAMTSKNMGFEV
                     SKLFKDIKTKETVKTFKKGSE"
BASE COUNT          797 a          552 c          456 g          901 t
ORIGIN      
        1 aggtcgcact ggtataaatg cttgtcaaaa taaacctgtg cagctttttc aaaaccatat
       61 atgttatttc ccatgtaaac ttcattaata tatgcctgta atatttcttt cttactaaaa
      121 tggcattcta accgtatagc ttcaataatt tctgccaact tgcttactac agtgttgcgt
      181 acaatctctt tttttgattt aacaagctgc tgggtaatgg ttgacccacc agatactatg
      241 tgcataacct gtagattttg atacagcgcc cgtgcaatcc cttttaaaga tataccccaa
      301 tgatcataaa aatttctgtc ttcagaaaat aacagcagat ctataaattc agatgggacc
      361 tcctgtattt taacagactg agatagtcct ttcttcccat cagtaatttc catcaaaacc
      421 aggccatgtc tatcgtaaat agtaatcgga gatttattgt ttgcggtaag gtatccatcc
      481 ttgtatggaa atattatata ggtaactatc gccactacaa aaacaaccaa acttgctttt
      541 aaaacgttgg cgctggtaca gtcaatatgt ttccaattat agcgcttcat agctactcta
      601 cagtaacagt attgctgaag tacagtatac caaacacttc agggttgtac atctcttcaa
      661 ccttacaggc agggagtttg aatgtgccag agtttgttgc tctcaccttg aatataactt
      721 tatgtgttcc acgctgtata aaatcagcat aatagataat cctgtcactg tactgctcat
      781 aatgattgaa cgtaccccag tttgtgtaat cactcttttt gtctactgct accttttctt
      841 cagtggcaaa tgtcacattg acaggttcaa atcctgaagg caactggtct tcaagcacag
      901 taatcccacg atccttagga gtatataccg ttatttcaac aatatnnnnn nnnnnctctg
      961 agcctttctt gaatgttttg acagtttctt ttgtttttat atctttaaat agtttgctta
     1021 cctcaaaccc catatttttt gacgtcattg cttcatatac aggaatatat tggtattgca
     1081 tggtaaagta acaggcaccc tttccatttt taataaaact gaattttttt acaccactat
     1141 cttctgatgt tacgatatat tgttttgcaa aatgcacatt gctttcatta acaattgcat
     1201 tcagcaattc ttttgtatca acaagcacct gaagtgaata atcaggttta tctttttcat
     1261 agcgtacaag atagctattg agagcccacc atactactgc attctgatgg gtattgagcc
     1321 atgcataccc tttgcgctgt ttcagcagat actgcacaat cttgtaatca ttattaaagg
     1381 ggatattggc ttccagtaga gcctgtaaaa tgagagcagt tgacaggacc gtatcataat
     1441 aaaaataacc ccattgcgat gtatcagtaa aatacacata tcccgactca gtccgcatgc
     1501 gggaaaagaa tttttcagca atttctttta ccagtgtttt ctttaatgtt gaatcaggat
     1561 ataatgcaag ggctttcagt agataagcat atgcggcaat gcaatcatca tattttttgg
     1621 ttattgtact gtatacaagc tttaattcat tttcactgat aaagccttgc aatgctgcaa
     1681 cataatgagc aaaagcaagt gtagtatagg tataccagga tgagttcctt ccctcatggc
     1741 taaatatgtt accttttttt acaaactgtt ttatcacctt aatggtatca ttatagattg
     1801 cattgtcaac atcatagcct ctttttttgg ccatggtcat aataaatgca gcatatactg
     1861 tgagatagct actgggaagc gaaccatctt tccagtatga aaatcctcca tttttgtagt
     1921 aagcaggaaa ttctttcaaa acatcagtaa ctacttttct gagttcatcc tttgactttg
     1981 tttttaaaag ccctttttga acaatgatat cctgacccaa aatgagtggt aaaatttttg
     2041 aagccttttg ttcaaaacat ccatagggat agtcaactaa ataatcaagg caacctttaa
     2101 gatccacaaa tgccgacgga gaagtaaaaa tattaatcga ggagacttca ggtataatat
     2161 ttctggttat ggttatctcc tgtacttctc tggactgtgg agctgaaatc cttccatact
     2221 gtgccactgt ttcaaataca ttaggagctt tgataggtat ggtatctgta atgccatctg
     2281 tataggttcc tgcctgtgct ttgaccgtaa aacctgccat acctgcttgt ccagttactg
     2341 taaccgggaa ggttacctcc tgagattgcc cattattaag aataagctct ttgacagcaa
     2401 ccgcagaacc atctttaagc ttcaagccat ttttcagttc caggcctatt gtaacgtttc
     2461 cctgtttgcc agtgtaattg aaaacaagag caccagcatt aaacgtatca ccaacacgaa
     2521 caaagcgcgg gaaggttggt agtaccatta aaggtttttt aacctcaaag gttgcttcac
     2581 catatccaaa ttttgaatct tctgtgtgaa caacaaccat cgatttgaag ctggtgaggt
     2641 tatcaggcag cttacattgc acctttgctt ttccctgttt taccacaact gttgccttat
     2701 aatatgcact gaaccg
//
LOCUS       DAOSZP010000300         2715 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_29092_length_2715_cov_3.352256, whole genome shotgun sequence.
ACCESSION   DAOSZP010000300 DAOSZP010000000
VERSION     DAOSZP010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2715
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29092_length_2715_cov_3.352256"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..657)
                     /locus_tag="PK348_07090"
     CDS             complement(<1..657)
                     /locus_tag="PK348_07090"
                     /inference="COORDINATES: protein motif:HMM:NF013046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HXK66015.1"
                     /translation="MKNPFYIIYDPAYTTSYVTASVEKPERVRQIMKELGDSYPIIKP
                     TSCTDEDILLCHSEGLLTMEKQDDVRFTVAKLAAGGAITAATMAMEGKPSFAVIRPPG
                     HHANPDHNWGFCYFNNMGIAIKRLLADSIIQKAIILDIDLHFGDGTDTIFKSTINVPV
                     LNIQSSNPVDFINETKTRLQQCGSADILGISAGFDQYIKDWGANLSTQDYFTIGQIAG
                     N"
     gene            complement(735..1322)
                     /locus_tag="PK348_07095"
     CDS             complement(735..1322)
                     /locus_tag="PK348_07095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66016.1"
                     /translation="MEVKDPINYYVEPVEIEIYLKKAGKVRTIIKDMYIELIDPEPLD
                     NDTSKKIFDYFKSRNEPIDVIEITNIFPELISIVFESYYHNINLYEKLSMYFKAGLSG
                     SADSWRLALYFTELLMKFEPTIASSQHIGDFQTYNLNYCIRKLNALGEKFLLEDSTVM
                     YLIKRRNKAYEGKPKDKEFEKLVELWQFNVKERPF"
     gene            complement(1340..>2715)
                     /locus_tag="PK348_07100"
     CDS             complement(1340..>2715)
                     /locus_tag="PK348_07100"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HXK66017.1"
                     /translation="NCIITIKNVKPATKDITIVYIHDPSGITQAIETAANLYIIDYEI
                     YKKAAMLCASLSPVIIVDSNRQGVPIKDVTYCIPSHQYDVRYYLDEKKLLSDMLSCCS
                     KEFAQAISEEDFDEIEKLLTQELSPHIVYNCIQTLRVILHSTTNRDLYKRIEKILYKM
                     QTRPLPDSYRYTIMLHKSYAYMTCQPVQVPQEYPFEAIEQLDEPSRPAPHTLPDICNR
                     IIEDRKRLEMLLDLFYANNTEIAKEAKSIEKAINKRKKEITQTPKLDVSLITKEKLKK
                     RLTVLKKAGIIVAGIAVAILIMVGSYHAFILYQEKQERERQARYIEYLSKKYAIHISD
                     RDIFFYANDVAVKNGYSRIDIKSLKEKNPHWIYPGNRFILLDGEVVIVKPGDTLWGIS
                     ERKLLSLNITFYTLLDELEKKIDKGIIDEHLAQKAMNAAIHTKHRERVKALLQKMNEL
                     KAKQNNT"
BASE COUNT          758 a          498 c          511 g          948 t
ORIGIN      
        1 attaccagct atctggccaa tggtaaaata gtcctgcgtt gaaaggtttg ctccccaatc
       61 ttttatatac tggtcaaatc ctgcactgat acccagtata tcagcactgc cacactgctg
      121 cagtcgtgtt tttgtttcat tgataaaatc aacagggttt gatgactgta tatttaacac
      181 aggaacatta atagtggatt tgaaaatggt atcagtacca tcaccaaaat gcaggtcaat
      241 atcaagaata atggcttttt gaatgataga atcagctaac aggcgtttta tagcaatacc
      301 catattgtta aaataacaga atccccagtt atgatcaggg ttggcatgat gtccgggtgg
      361 ccgtataaca gcaaagctgg gtttgccttc cattgccatg gtagctgcgg taattgcacc
      421 acctgcagca agctttgcta ctgtaaagcg cacgtcatcc tgtttttcca tggtaagcaa
      481 gccttcagaa tggcagagaa gtatatcctc atcagtgcag cttgtgggtt ttattatggg
      541 gtaactatcg cccaattcct tcattatttg tcggacgcgt tccggttttt ccactgaagc
      601 agttacatag ctggttgtat atgcagggtc atagataatg taaaatggat ttttcataaa
      661 aacatcactc gcaacattca cgttatcgat atgcttctat agtaaatgat tttgaaataa
      721 atgtcaaata cgttttaaaa gggacgttct ttgacattaa attgccatag ctcaaccagt
      781 ttttcaaatt ccttgtcttt aggttttcct tcataggctt tgttccgccg tttgataagg
      841 tacataacgg tactatcttc aagtaaaaat ttttcaccca atgcattgag tttgcggata
      901 caataattaa gattatatgt ctgaaaatca cctatatgct gcgacgatgc tatagttggc
      961 tcaaatttca ttaatagctc tgtaaagtat aatgcaagtc gccatgaatc ggcgctacca
     1021 gaaagacctg ctttaaaata catgctcagc ttttcatata aattaatatt gtgatagtat
     1081 gattcaaaaa ctatagatat caattccggg aaaatgttgg taatttctat tacgtcaatg
     1141 ggttcattgc ggcttttaaa ataatcaaaa atctttttac tggtatcatt atccagcggt
     1201 tccggatcaa taagttcaat atacatgtct tttatgatag tgcggacttt acctgctttt
     1261 ttcaggtata tttctatttc aaccggctct acgtaatagt ttatagggtc tttaacttcc
     1321 atgcagtgta accatgacct taagtattgt tttgtttagc ttttaattca ttcatcttct
     1381 gtagtaatgc tttaaccctt tctctgtgtt tagtatgtat tgctgcattc atggcttttt
     1441 gtgccagatg ctcatcaata atacctttat caattttttt ctctaattca tccagtaagg
     1501 tataaaaggt aatattgagt gaaagtaatt ttctttctga tattccccac agagtatcac
     1561 ctggtttaac gatgacaact tccccatcta aaagaataaa tctgttgcca gggtaaatcc
     1621 agtgtggatt cttttcttta aggcttttaa tatctatacg actgtagcca ttctttactg
     1681 caacatcatt ggcatagaaa aagatatccc tatcagagat atggatagca tattttttac
     1741 tgaggtattc aatatagcgg gcctgacgct cgcgctcctg cttttcctga tacagtataa
     1801 acgcatggta gcttcccacc ataatgagta tagctacagc tatgccagct acaataatcc
     1861 cagctttctt cagtacagtt aaacgctttt tcaacttctc tttagtaatg agtgatacat
     1921 ctagctttgg tgtttgcgta atttcttttt tccgtttgtt tattgctttt tcaatgcttt
     1981 tagcttcttt tgcaatttct gtgttgtttg catagaacag gtctaatagc atttctaatc
     2041 gtttgcggtc ttcaataata cgattacata tatcaggcag tgtatgtggt gcaggtcgtg
     2101 atggctcatc taattgttca atggcttcaa atggatattc ctgaggtacc tgcactggtt
     2161 ggcacgtcat atatgcatat gatttatgca gcataatcgt gtaccggtaa ctatcgggca
     2221 atgggcgtgt ttgcatttta tacagtattt tttcaatacg tttgtacagg tcgcggttag
     2281 ttgttgagtg gagtataaca cgcagtgttt ggatgcagtt gtacactata tggggcgata
     2341 gttcctgtgt aagaagtttt tctatttcat caaaatcttc ttcggaaata gcttgtgcaa
     2401 attccttgct gcaacaagaa agcatgtcag ataacagttt cttttcatca agataatacc
     2461 gtacatcata ttgatgcgaa ggaatgcagt aggtaacatc tttaatggga actccctgac
     2521 ggttactatc gacaataata acaggtgaaa ggcttgcaca aagcattgct gcttttttat
     2581 aaatctcata gtcaataata tacagattag cagctgtctc tatggcctga gtgatgcctg
     2641 atgggtcatg tatatagaca atggtaatat cttttgtggc aggttttaca ttttttatgg
     2701 taatgatgca gttat
//
LOCUS       DAOSZP010000301         2712 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_29138_length_2712_cov_3.089198, whole genome shotgun sequence.
ACCESSION   DAOSZP010000301 DAOSZP010000000
VERSION     DAOSZP010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2712)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2712)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2712
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29138_length_2712_cov_3.089198"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            314..2269
                     /locus_tag="PK348_07105"
     CDS             314..2269
                     /locus_tag="PK348_07105"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HXK66018.1"
                     /translation="MKKYIVLCCSIILCAMPLSAQDFTIIQTTDLHSHFLGFSPNIDY
                     TPNTINDDATVGGFARIATVINNIKKSRKNPVLLLDSGDFLMGSVFHLLCREEAFELR
                     LMKKMGYDVITLGNHEFDLYPDGLARIIRAAQKYNALPAIVASNIVFSADSKDDDSLE
                     KVFEDELVKPYTVITKEKLKIGFFGLIGKDAAEVSPFAHPVTFADQVQTAKQMVDILR
                     NKEKVDVVICISHSGIRKYQDKDEIEPGRSEDVQLAKEVKGIDVIVSGHTHVKIKQPI
                     IVGKTIITQSYEYGKQVAVLDLSLSKDGVVLKNYTYIDINDSIKGDPAITALINQFVQ
                     RINQQVLSPLKLTCDSVLAKTKFDLVIKEEETNLGNLIADSIRWYVNSVDFDPKDPDS
                     RVAIGVISNGVIRDNIVVGKTGKVVLTDAFATIPLGIGMDEKKTMGYPLITCYVYASE
                     IKKALEILTSIYPLKGSDYFIQVSGVKFTYNPNRMLFDRVTEIWIGDEQNGYVPLDYS
                     DNNKKLYRIAADIYNATFLKIIGNFTWHILDIVPKWRDGKPIEKLTEARVDTDKRKPG
                     IQEAKEWIGVIEYIRSFKDEDGDGTPDIPAMYQGKLGRIVVEASLNPYHLLKRGTKVT
                     WIGFIIFLIVVAIVTVVVVVVIKRFKR"
     gene            2466..2705
                     /locus_tag="PK348_07110"
     CDS             2466..2705
                     /locus_tag="PK348_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008287623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HXK66019.1"
                     /translation="MSLSKITKKGQITLPAKLRKKLNTDEVHIKMVDDVIIITPAKKL
                     GGIFKKYAKKDIPIDTLLKKEKEAFADAITEKHTH"
BASE COUNT          900 a          447 c          562 g          803 t
ORIGIN      
        1 cacagggtac aaagattttt tagataaatt gctctgaccc cttactgtag cgtagtcagc
       61 gattttcgtg cacactacaa gagagggaaa ttccacactc agagatgcaa agactccagt
      121 ggttgtatta tgatgctctg acccccgtta atcagcgcac gtagcgatct ctgtgcactt
      181 agcgtgatta tccctttaat cagtattgat gataaatttt atttatcatc aatattttat
      241 acttgataaa aaattgttac tgcaactaca ataactgaca ctatacgtaa taccatacag
      301 ggggtttggt accatgaaaa aatatatagt actttgctgc agtatcatac tttgtgcaat
      361 gccattatct gcacaggatt ttactataat tcagacaact gacctgcatt cacatttttt
      421 agggttttca cccaatattg attatacacc taatacaata aatgatgatg ctaccgtcgg
      481 tggttttgca cgtattgcta ctgtcatcaa taatattaag aaaagccgaa aaaatcctgt
      541 actgctgctt gattctggcg attttttaat gggatcagtg tttcatcttt tgtgccgtga
      601 agaagccttt gaactgcgcc tcatgaaaaa aatggggtat gacgtcatta cactgggtaa
      661 tcatgaattt gatctgtatc ccgatggcct ggcgcgcatc atacgggcag cacagaagta
      721 caatgcattg cctgcaattg ttgcaagcaa tattgtattc agtgccgata gcaaggatga
      781 tgatagcctt gaaaaagtct ttgaagacga gttggtcaaa ccatatactg tgataacaaa
      841 agaaaaatta aagataggct tttttgggct tattgggaaa gatgctgcgg aagtttcacc
      901 atttgctcat cctgtaacct ttgctgatca ggtacaaaca gcaaaacaga tggtggatat
      961 attgcgaaac aaagaaaagg ttgatgtagt aatatgcatt tcgcacagtg gcatcagaaa
     1021 gtatcaggat aaagatgaaa ttgaacctgg acgttccgaa gatgtgcagc tggcaaaaga
     1081 agtcaaagga attgacgtta tcgtcagtgg gcatactcat gtaaaaataa aacagccaat
     1141 tattgttggc aaaacaatca ttacacagtc gtatgagtat ggcaagcagg ttgctgttct
     1201 tgacttgagt ttatcaaaag atggtgtcgt attaaaaaac tatacatata ttgacatcaa
     1261 tgattctata aaaggggatc cagcaataac tgctctcatc aatcagtttg tgcaaaggat
     1321 taatcaacag gtactatcgc ccctgaaact tacatgcgat agtgtgcttg ccaaaacaaa
     1381 atttgattta gtcatcaaag aggaagaaac taacctgggc aaccttattg ctgattcaat
     1441 acgctggtat gttaacagcg ttgattttga tccaaaggat cctgatagca gggtagcaat
     1501 aggtgtaata tctaacggtg tcattcgcga caacattgtg gtgggtaaaa caggtaaagt
     1561 ggtgctgacc gatgcatttg ctactattcc gcttggcatt ggcatggatg aaaaaaagac
     1621 tatgggctat ccactgataa cctgctatgt gtatgcgtcg gaaattaaga aggcacttga
     1681 aattttaaca agcatctatc cactaaaagg gagcgattat tttattcagg tttcaggtgt
     1741 caaatttacc tataatccca accgaatgct ttttgatcgc gtgacggaaa tctggattgg
     1801 cgatgaacaa aacggctatg tgccacttga ttattcggat aataataaaa agttatatcg
     1861 tattgctgct gatatctata acgcaacgtt tttaaaaatc attggtaact tcacctggca
     1921 tattctggac attgtaccca aatggcgtga tgggaaacca atagaaaagc ttaccgaagc
     1981 acgtgttgat actgataaac gaaaacctgg catacaggaa gcaaaggaat ggataggtgt
     2041 tattgaatat attcgtagtt tcaaggatga ggacggtgat ggcactcctg atatccctgc
     2101 tatgtatcag gggaagcttg ggcgtatagt tgttgaagca agcttgaatc catatcattt
     2161 actcaagcgc ggaacaaagg ttacctggat tggatttata atctttttaa tagtggtggc
     2221 gatagttact gtggtagtgg tagttgtgat taaaagattc aaaaggtagt aataagatgg
     2281 taggaagagc gttctctgtg tgatgtgctt gttgaatgct atgatataca gagaacgctg
     2341 tatattcccc acattacaat gctattacga aattcactta ttatagttaa tataataaat
     2401 gtttgacaaa attaaattat gtattacttt atatgtaaag taatacattt taaagggagt
     2461 aatgtatgtc attatctaaa attaccaaaa aagggcagat tacattacct gcaaaattaa
     2521 gaaaaaaact taatactgat gaagttcaca taaagatggt tgatgatgta attattataa
     2581 cacctgcaaa aaaattaggt gggattttta agaaatatgc aaaaaaagat attcctattg
     2641 atacattgtt aaaaaaagaa aaagaggcat ttgccgatgc aatcacggaa aagcatactc
     2701 attgatgcaa at
//
LOCUS       DAOSZP010000302         2710 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_29160_length_2710_cov_2.941243, whole genome shotgun sequence.
ACCESSION   DAOSZP010000302 DAOSZP010000000
VERSION     DAOSZP010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2710
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29160_length_2710_cov_2.941243"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(331..1848)
                     /locus_tag="PK348_07115"
     CDS             complement(331..1848)
                     /locus_tag="PK348_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018250086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HXK66020.1"
                     /translation="MLSQALSLVIHGIDAHLIDVEVDIVKGLPNFTIVGLPDSIIRES
                     KDRIRSAIENSGFEFPPKNFIVNLAPAGYRKEGANLDLPIAVSILIATGQVESARVDI
                     PMVGELSLDGRVRPIKGVTSMAIALYRAGIHEFIVPYENRNEATAIEGIKVYPVKDIA
                     QAVEACCGKIQAYTHHESGDSSSNGYLDMASVMGQESAKRALEIAAAGRHNILLYGPP
                     GSGKTMLAKCVPGILPPLTKEQAITTTMIHSVGGTLGHGQGLIYTPPFRAPHHTSSDA
                     ALVGGGKIPTVGEVSLAHNGVLFLDEFVEFRNDVIQALRQPLEDCNITISRAMGTMTF
                     PADFMLVASSNPCQCGYLFDPEIPCKCSPGKIRSYFSKIAGPILDRIDIEVLVGRVPY
                     KDLLGKGNAESSEVVRQRVIKARDIQARRFKGRTTTSNGRMTSDEVKEFCAINAECES
                     ILELAIKRMNLSARSFFRILKVSRTIADLDNSETIEKRHILEALSYKNLQRYYDI"
     gene            complement(1921..2154)
                     /locus_tag="PK348_07120"
     CDS             complement(1921..2154)
                     /locus_tag="PK348_07120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66021.1"
                     /translation="MLLQRAVTEIEGVRPPIITIEIFTKLDEMRAYRHKFRNIYLYLI
                     APARIIELANEGIALVEKFNTEIYEFCSFLKKN"
BASE COUNT          769 a          561 c          492 g          888 t
ORIGIN      
        1 caaagtttat gcggtatgag gagtattacc ggaaaaaata ttcgtatgta gtgaattggg
       61 tgtaaaaaaa aatagaaatg gggtcaaagc cataacgtgg caaaatcaat acagttgagg
      121 aatattatca taagaaatat tccataacat acttgagcgg ttctaaaaag cacaaaaaag
      181 aggtcaaagc ataaaaatat gccaagaacc ttgctttttg ttagtcaatt ttgaaatata
      241 atctcttttt tgcatggaat caattgcctt ccatgtattc tttagttttc atacatacat
      301 ctaaccttca aataagcaaa cgccttttta ctaaatatcg taatagcgct gtaaattttt
      361 atatgacagt gcttctaaga tatggcgttt ttcaattgtt tctgagttgt ccagatcagc
      421 aatggtgcgg ctcactttta atattctgaa aaagcttcgt gcagaaagat tcattcgctt
      481 tattgcaagt tccagtattg attcgcattc agcgttgatt gcacaaaatt cttttacctc
      541 gtcgctggtc atgcgtccat tgctggtggt tgttcttccc ttaaatctcc gggcttgaat
      601 atcacgcgcc tttattacac gttgacgtac tacctcagaa ctttcggcat tgccttttcc
      661 cagcaaatcc ttatatggca ctcttcccac cagcacttca atatcaatac ggtcaagtat
      721 gggacctgct attttactga agtagctacg gattttgcct ggcgaacact tgcaagggat
      781 ctctggatca aaaagatacc cacactgaca gggattgctt gatgcaacca gcataaaatc
      841 ggcagggaat gtcatggttc ccattgcccg cgatatggtg atattgcaat cttccagtgg
      901 ttgacgcagt gcctgtatta catcgtttcg gaattccaca aattcatcca aaaacagcac
      961 accattatgt gccagtgata cctcacccac ggtgggaatt ttgccgccgc caacaagtgc
     1021 cgcatcagaa ctggtatggt gtggcgccct gaatggcggt gtgtatataa gtccctgccc
     1081 atgtccaaga gtaccaccaa ccgaatgtat cattgtggtt gtgattgcct gctctttagt
     1141 aagcggcggc aaaatccccg ggacacattt tgcaagcatg gtcttccccg agccaggagg
     1201 tccatacagc aatatattgt gtctgcctgc agctgctatc tccagtgcac gtttggccga
     1261 ttcctgcccc ataacgcttg ccatatcaag gtacccgttg ctggaactat cgccactttc
     1321 atgatgagta taggcctgta ttttcccaca acaggcttcc actgcctgtg caatatcttt
     1381 tacgggatat acctttattc cttctatagc tgttgcctcg ttgcgatttt catacgggac
     1441 aataaactca tggatgccag ccctatacag cgctattgcc attgatgtga ctcctttgat
     1501 ggggcgcact cttccatcca gggatagctc cccaaccatg ggtatatcaa cgcgtgcact
     1561 ttccacctgc cctgttgcta taagtattga aactgcaatg ggtaaatcca ggttagcacc
     1621 ttccttgcga tagccagcag gggctaagtt gacaataaaa ttttttggtg ggaattcaaa
     1681 accactattt tcaattgccg agcgaatcct gtcttttgat tcgcggatga tggaatcagg
     1741 cagaccaaca atagtaaagt ttggcagccc tttaacaatg tccacctcta catcaataag
     1801 atgcgcatca atgccatgaa tgacaagcga taaagcctgt gaaagcataa gtattcctcc
     1861 gtgcgtgttt tatatataca acgcatgagg aagaaaaaat ttaaaaaatt ttgagatttt
     1921 ttaatttttt tttagaaacg aacagaactc atatatttca gtattaaatt tctccactag
     1981 tgctatccct tcgtttgcca gttctattat acgtgcagga gcaatgagat acagatatat
     2041 atttctaaat ttatgcctgt atgctcgcat ttcatctaat tttgtaaata tttctatagt
     2101 tataattggt gggcgaacac cttctatttc tgtaacagcc ctttgtaaaa gcaagctgtg
     2161 ataatctttt cctagtggga tatcgccatc aatggttcgt ataatccttt caagaatatt
     2221 ctcacaacct gtatatattg aatgtaatga cccagctaat gcttttatat gtgaatcaat
     2281 attttcataa tcttttttaa gtttctaaac gttttaattt cctcatgata ctattttttt
     2341 ctttttgtaa ataggttata tcagcttata tatcagctat gagggctttc atagtttaca
     2401 ttcttttttt attttttgaa taatctcagg gcgtatacta tcatcgtcga ataataaatc
     2461 aaatgtaatt tctctaaata tttcttcaag gccagtgaac aattcttttc ttttttcttg
     2521 agttaatcac tgaacatata tatctacatc tgacatttca tcaaaacatt caagatgaag
     2581 gagtaaagca aagacttcta ttttaatttt tttgccttta tgataatatt gtttcatcca
     2641 tcagcccgaa tagaggcgtt ctttgctaat gtaattgttt atatctttgt caaaagaata
     2701 aactgtatat
//
LOCUS       DAOSZP010000303         2708 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_29187_length_2708_cov_2.664908, whole genome shotgun sequence.
ACCESSION   DAOSZP010000303 DAOSZP010000000
VERSION     DAOSZP010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2708
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29187_length_2708_cov_2.664908"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /locus_tag="PK348_07125"
     CDS             complement(<1..217)
                     /locus_tag="PK348_07125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66022.1"
                     /translation="MRNIIYLLPGIFLFIACANKPLIIIKAECGSSVSMNGTIANDSL
                     TYTFKPCTHCRSLKDVSNYIYFQGDTLC"
     gene            complement(210..1127)
                     /locus_tag="PK348_07130"
     CDS             complement(210..1127)
                     /locus_tag="PK348_07130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66023.1"
                     /translation="MALNQINNYIRLIDAQNANKTGNIYISVLKNEFIMLNEEISIPK
                     IPVSKLSYIEALPIAQTIIPLIPLFLFGHTLLEERQPAHELHSLHFIRLLEGRCINFY
                     HVLRVDFKFGGDSSAILEPGNNDYYPSYRTNRLYYKSRLVPTFKDPSPPITPIKLIQS
                     ITTESDQYFHTYAMFDDIDTSNITNEFIKTLPDIFSIPSNLYSFIVMDYYTACMNIPN
                     PIPLELDRAVIIFEPLFFIIASHFIPIDSIISLHELEAHFPELIAIKDQKLVPTPNLI
                     QMAKEYFNRYSLTRDEQCMLKGWWQLVIA"
     gene            1257..1736
                     /locus_tag="PK348_07135"
     CDS             1257..1736
                     /locus_tag="PK348_07135"
                     /inference="COORDINATES: protein motif:HMM:NF015908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HXK66024.1"
                     /translation="MDNLINIGVAQLAVAENPAVLRTILGSCVGICIFERNKKIGGLA
                     HILLPKNPGDSNPEKYADTAIPILVQQLLKKGCKKEFMSAKIAGGASMFKFKSTTTLA
                     NIGENNIEATRQALAKFGIPIVAEDIGGSAGRVIDFFLDDGRLKVKASGQEKMYYKV"
     gene            1760..2620
                     /locus_tag="PK348_07140"
     CDS             1760..2620
                     /locus_tag="PK348_07140"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HXK66025.1"
                     /translation="MHEHIQQRIQSIIQNVDQLPSLPDVVSRIINMVNDPNVSFKQVA
                     EEIAKDQAITANILKLCNSAYFSKGKEISSIDRAIVILGLKEVKDIVVLATTKSVLNR
                     VILGYDLARGELWKHGVAVAMLAKKIANDCNQKTVADIAFTGGIIHDVGKTVLALFVQ
                     STFKDILSTVTEKNITFQEAEKLIMGFDHQQIGQQVTIKWKFPKVLQSIVRYHHEPTN
                     APDEHKKIVSIVHIANTLCLMAGIGIGSDGLYHELNQDAVTMLGLQDAQLEKFYSDIP
                     ELMNKARDIL"
BASE COUNT          882 a          422 c          581 g          823 t
ORIGIN      
        1 aacacagcgt gtcaccctga aaatagatgt aattgcttac atctttcaat gacctgcaat
       61 gagtacatgg cttgaaggtg taggtaaggg aatcattggc gatagttcca ttcattgata
      121 cactgctgcc acactcagcc ttaataatta tcagtggttt gttggcgcag gcaatgaaaa
      181 gaaagatacc tggcaaaaga tatataatgt tacgcaataa caagctgcca ccatcctttc
      241 agcatacatt gttcatctcg ggtgagtgaa tagcggttga agtattcctt tgccatttga
      301 atcagattgg gtgtgggtac aagcttttga tcttttattg caatgagttc agggaaatga
      361 gcttctaatt catgtaaaga aataattgaa tcaattggta taaagtgtga agctatgata
      421 aaaaaaagtg gttcaaaaat tataactgca cgatcaagtt caagagggat ggggttggga
      481 atattcatac atgcagtata gtaatccatg acaataaaag agtaaagatt tgatggtatt
      541 gaaaaaatat cgggtaatgt ttttataaat tcgttcgtta tatttgatgt gtcgatatca
      601 tcaaacattg catacgtgtg aaaatactgg tctgattcgg tagtgataga ttgaataagc
      661 tttattggtg ttattggcgg tgaaggatct ttaaatgtag gcacaagtcg tgatttataa
      721 taaagcctgt tggttctgta tgatggatag tagtcattat tgcctggttc aagtattgca
      781 gagctatcgc caccaaattt aaaatccacc cgcaatacat gataaaaatt aatacacctg
      841 ccttcaagca gtctaataaa atgaagacta tgcagttcat gtgctggctg acgctcttcg
      901 agcaaggtat gaccaaataa aaaaagcgga ataagaggga tgatagtttg tgctataggt
      961 aacgcttcga tatatgataa ttttgatacc gggattttag ggatgcttat ttcttcattg
     1021 agcatgataa attcgttttt caaaacactt atatatatat tcccggtctt attagcattt
     1081 tgagcatcta taaggcggat ataattgtta atctggttta atgccattcc cacttctcct
     1141 ggtaaattta ttgtaataaa tattgccttt tgccttgaca aatatacata atgaagtaat
     1201 tttttatcaa gataatattt aatggaaatg gcaatttttt ataattggta gacagaatgg
     1261 ataatcttat caacattggt gtagcacagc ttgcagttgc tgaaaaccct gcagtgttgc
     1321 gtaccattct gggtagctgt gttggtatat gtatttttga gcggaataaa aagatagggg
     1381 ggcttgctca tatattatta cccaaaaatc cgggtgatag caatcctgaa aaatatgcag
     1441 atacagcaat ccctattctg gtacagcagt tattaaaaaa agggtgcaaa aaggaattta
     1501 tgtctgcaaa aattgcaggt ggtgcttcaa tgtttaaatt taaatcaaca acgacgctgg
     1561 caaatattgg tgaaaacaat attgaggcaa cacggcaggc actggcaaaa tttggtattc
     1621 ctattgtagc tgaagatatt ggtggcagtg ctggcagggt tattgacttt ttcctggatg
     1681 atggaaggtt aaaggttaaa gccagtggcc aggaaaaaat gtattataag gtttgataga
     1741 tggattggag ataataatta tgcatgaaca tatacaacag aggattcagt ccataattca
     1801 gaatgttgac cagttacctt ctttgccaga tgtggtaagc aggataatca atatggtcaa
     1861 tgaccctaat gtctcattta agcaggttgc cgaagaaata gcaaaagatc aggcaatcac
     1921 cgccaatatt ttaaaattgt gcaattcggc ttatttcagc aaaggtaagg aaatatcttc
     1981 tattgaccgt gctattgtca tcctggggct taaagaagta aaagatattg ttgttcttgc
     2041 cacaacaaaa tcggtattaa acagggttat tttaggttat gacctggcac ggggtgagtt
     2101 gtggaaacat ggtgttgcag ttgcaatgct tgcaaaaaag attgcaaacg attgtaacca
     2161 gaaaacagtg gctgatattg cttttacggg tgggataata catgatgtag gtaaaacggt
     2221 tctggcatta tttgtccaga gtacatttaa ggatattctt tccactgtaa ccgaaaaaaa
     2281 tatcacattc caggaagcag aaaaattgat tatgggattt gaccatcagc agattggcca
     2341 gcaggttacc ataaagtgga aattccccaa agtgctacaa tcaattgttc gctatcatca
     2401 cgagcccaca aatgctcctg atgaacacaa aaaaatagta tcaatagtcc atatagcaaa
     2461 tactctgtgt ctcatggcgg gcattggtat tggcagcgat gggttatatc atgaattgaa
     2521 tcaggatgca gttacaatgc ttggactgca ggacgcacaa ctggaaaaat tctacagtga
     2581 tatcccagaa ttgatgaaca aagcaaggga tattctttag gatttacgta tgcatattat
     2641 aaaaccttgc cctgcctgcg gcattaagct gcgttttcct attgattctg gtaaaataaa
     2701 agtacaat
//
LOCUS       DAOSZP010000304         2700 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_29284_length_2700_cov_4.012854, whole genome shotgun sequence.
ACCESSION   DAOSZP010000304 DAOSZP010000000
VERSION     DAOSZP010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2700
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29284_length_2700_cov_4.012854"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..530
                     /locus_tag="PK348_07145"
     CDS             <1..530
                     /locus_tag="PK348_07145"
                     /inference="COORDINATES: protein motif:HMM:NF014207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HXK66026.1"
                     /translation="AVKQKGRTEDSPKLDKKGQATDRHDAFIQVNAKQNLSTSATVTK
                     PVVHDQLMQLLSKAKVMQEGEKTSLSLKLYPESLGKLSVNLGLEHGILSGRFIVESQE
                     AKELLLQQLESIRWELQNNGVQVGAFEVNVKEHHQREFTEMPGLSHKQTIENTEYEVA
                     SNRYIYHDGLLDVII"
     gene            541..1155
                     /locus_tag="PK348_07150"
     CDS             541..1155
                     /locus_tag="PK348_07150"
                     /inference="COORDINATES: protein motif:HMM:NF015896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HXK66027.1"
                     /translation="MPDALKMSGEDVAKAQFQANEVNAKLKAKKGKLPSELDKDSFLK
                     LLVAELRHQDPTQPMNDREFISQMAQFSSLEQMSNMNQSIEKLVLRSQSSDAFSLIGK
                     EVEAVDFASQQIVRGLVSGVVYRQDGVKVIIGSAEVSLNDIHAVYLPGNYRTEKNDAA
                     ANTDVTVKQEIKENVAQEKQNAVEAYSTNAFSKGSSSHIEPVRN"
     gene            1176..2546
                     /gene="flgE"
                     /locus_tag="PK348_07155"
     CDS             1176..2546
                     /gene="flgE"
                     /locus_tag="PK348_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010575599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HXK66028.1"
                     /translation="MMRSLFAGVSGLKNHQTRMDVIGNNISNVNTYGFKYSRVTFQDM
                     LSQTISGAAKPEENRGGINPRQVGLGMNIAAIDKIFTQGSLQTTGVNTDMAISGDGFF
                     IVADGQKHLYTRAGTFALDKDGTLVNPANGLKVQGWMATTNAAGEKVINTSGTVEDII
                     VPIYGKVPAKETNYVKYKCNLDSKLPVVPNDANAIMRTSAGVTTNIDIYDKLGNPHRM
                     TLNFWKSGVNEWTASAAITDTELVTLDVPNGGNQPNQINGSTRVVLKFSPEGRIVSVA
                     DANSPDEINQGKLEVNLSYRVAGDPTLRNIRLDFGSSGLVEGITQFSSPSTTKAVEQD
                     GYAMGYMESFTVDNSGIITAVYSNGTKQMIGQVATAVFTNPQGLIATGDNMFEVSNNS
                     GLPLIGPAGEAGRGKIVAGTLEMSNVDLSEQFTDMIITQRGFQANSRTITTSDQMLQE
                     LINLKR"
BASE COUNT          859 a          506 c          648 g          687 t
ORIGIN      
        1 atgcagtgaa acagaaaggg agaacagaag atagcccaaa gctggacaaa aaagggcagg
       61 caactgaccg tcatgacgca tttattcagg taaatgcaaa gcaaaatttg agtaccagcg
      121 ctactgttac aaaacctgtg gtgcatgacc agctgatgca gcttttaagc aaagctaaag
      181 taatgcagga aggtgaaaaa acaagccttt cattgaaatt atatccagaa agtttaggta
      241 aactttctgt aaacctggga ctggaacatg gtattttgag tggtcgtttt attgttgaaa
      301 gccaggaggc aaaagagctc ctgcttcaac aactggaatc aattcgctgg gagttacaaa
      361 acaatggtgt gcaggtaggt gcgtttgaag taaatgttaa agagcatcat cagcgtgaat
      421 ttactgaaat gccaggattg tcacacaaac aaactattga aaatactgaa tatgaagtgg
      481 caagcaaccg atatatatac catgacggat tactggatgt tataatataa ggagcataca
      541 atgccagatg cattaaaaat gtcaggcgaa gatgtagcaa aagcacaatt tcaggcaaat
      601 gaagttaatg ccaaattgaa agctaaaaag ggtaaattac catcagagct tgataaggac
      661 tctttcttga agcttttagt ggctgaactg cgccatcagg accccaccca gcccatgaat
      721 gaccgagaat ttataagtca gatggcacaa ttctcttcgc ttgaacagat gagcaatatg
      781 aatcagtcaa ttgaaaagct ggtgctacgt tcccagtcca gtgatgcatt ttcacttatt
      841 ggcaaagagg ttgaagcggt tgattttgca agccagcaga ttgtacgcgg gttggtaagc
      901 ggtgttgtgt acaggcagga tggagtcaag gtgattattg gttctgctga ggtttcgctc
      961 aatgatatcc atgcggtcta tttacccggt aactatcgca cagaaaaaaa tgatgctgcc
     1021 gcaaacactg atgttacagt aaaacaggaa ataaaggaaa acgtggcaca ggaaaaacaa
     1081 aacgccgtgg aagcatattc aacgaacgcc ttcagtaaag gcagttcatc acatatagaa
     1141 ccagttagaa attagattaa caggagtttt gcgatatgat gcgatcactt tttgcaggtg
     1201 tttcaggact taaaaaccat cagacacgga tggatgtcat tggcaacaat atttccaacg
     1261 ttaataccta tggcttcaag tacagcaggg ttacctttca ggacatgctg tcgcagacta
     1321 tttcaggtgc tgctaagcct gaagaaaacc gtggcgggat taatccccgg caggttggtc
     1381 tgggtatgaa tattgctgct attgacaaga tatttactca gggtagcttg caaacaactg
     1441 gtgtcaatac agacatggca atatccggtg atggattctt tattgttgct gatggacaaa
     1501 agcacctgta tacacgtgct ggcacatttg cacttgataa ggatggaacg ctggtaaatc
     1561 ctgcaaacgg acttaaagtg cagggatgga tggctacaac aaatgctgcc ggggaaaagg
     1621 ttatcaatac atccggtacg gttgaggata tcattgttcc tatttatggg aaagtgcctg
     1681 caaaagaaac caattatgta aaatataaat gtaatctgga ctcaaagctg ccggtagtcc
     1741 ccaatgatgc caatgcaata atgagaacat ctgctggagt gacaaccaat attgatatct
     1801 atgataagtt gggtaatcca caccgtatga cgctcaattt ctggaagagt ggggtgaatg
     1861 agtggacggc ttctgctgct atcacggata ccgaactggt aacactcgat gtgcccaatg
     1921 gtggcaatca gccaaatcaa ataaacgggt caaccagggt agtgctaaaa ttttcacccg
     1981 aaggccgcat tgtatctgtt gctgacgcaa acagtcccga tgagattaat cagggcaaac
     2041 ttgaagtcaa tttgagctat cgtgtagcag gtgaccccac actacgtaat atcaggcttg
     2101 attttggcag ttctgggctt gttgaaggta taacccagtt ctcatcccca tcaaccacca
     2161 aggcggttga acaggatggg tatgctatgg gctatatgga atcatttact gtggataact
     2221 cgggcatcat cactgctgtg tattctaatg gcaccaagca gatgataggg caggttgcca
     2281 ctgcagtgtt taccaatcct caggggttaa tagcaacggg cgataatatg tttgaggtat
     2341 ccaataactc agggctaccg ttaataggac cagcaggtga ggcaggcaga gggaaaattg
     2401 tggccggtac acttgaaatg tcaaacgttg acctttctga gcagttcact gatatgataa
     2461 ttacccagag aggattccag gcaaattccc gaactatcac aacatctgac cagatgcttc
     2521 aggaattgat taatctgaag cggtaatata aatagtaggg aatggggttt tttcaaaacc
     2581 ccattgttgt aaacagatgc ttcaggaatt aatcaatctg gaggggtact ataaatagta
     2641 aggggtgggg ttttttcaaa accccattgt tgtaaacaga tgcttcagga attaatcaat
//
LOCUS       DAOSZP010000305         2698 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_29315_length_2698_cov_3.074537, whole genome shotgun sequence.
ACCESSION   DAOSZP010000305 DAOSZP010000000
VERSION     DAOSZP010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2698
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29315_length_2698_cov_3.074537"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..460)
                     /locus_tag="PK348_07160"
     CDS             complement(<1..460)
                     /locus_tag="PK348_07160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66029.1"
                     /translation="MKLLQNPYIRNNSITIIQALIIATSVIALFHVFHLLHIIIIAFI
                     VSLGLQVWCQLNPCYADYITSAEAPIASLALLITMLISHSMSLSIVVTFIVLFIYHYF
                     FSSYELYHDIYFTALLFSIILMFVSLYLNNIGVFNEYTIKIFTGVQSNTSS"
     gene            complement(426..1964)
                     /locus_tag="PK348_07165"
     CDS             complement(426..1964)
                     /locus_tag="PK348_07165"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXK66030.1"
                     /translation="MKHAFVYTVCSCILLSVIIAFATPARAGSYYEAGCRYYVHKNWE
                     KAKENFLKDIETTDRGDSYYFVGEIEKNNGNLTTALEYFEKAVERKMTPKYRNLAYWN
                     LIVIYESRSDFPKTIKTCKYFYEVLGEASAKSKAENLINKLMWTENEDAKKEYEEGIN
                     SKSKDYSLALQHFRQAIMLDSAFLSPVFEIGMYYYTQNQYRDALSYFNQILYRIPFYG
                     ELQLLVGEIYFQEKRYSEAATAFEAALEYAFIDKDMKYSSQLKVATAYYNIGQYTKAE
                     TFLQQAISTKKGNLSPYLLLSAIQMQKNDYDGALQTLQAAYKIDSNNPEVLYQLGLVF
                     YNKNDNKAYEYFEKVYMQYRQKHKEIPAKYIKAFIITAQYYYTTKQYSKVVELTTIVP
                     ENLRDESLSLNYARSLYMLAQYNEAIVHFNKFRLSDNDLFILCKCYAFINDTSKVKEI
                     LSSNYYNTNFINKVKEDKLLKPIYTEVQKQRETPATVQPQHQEEQKDLPTTHETTTEP
                     IHPQ"
     gene            2135..>2698
                     /locus_tag="PK348_07170"
     CDS             2135..>2698
                     /locus_tag="PK348_07170"
                     /inference="COORDINATES: protein motif:HMM:NF015013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HXK66031.1"
                     /translation="MNSVRSTTHIAIATALSRLLGFVRDMLIAWMFGASWQIDAFFVA
                     FRIPNLVRRLVAEGTLSLPLVSVIMSYRHKEERKIIANDLFSLYIMSGFLISVTGIVA
                     APAIVKILGFGFTGSVYSLSVVLLRIMFVYCLCASIVAYAMGVLNTEGVFFMPALSPL
                     LLNVGIILFCFIAYLYSSVMWLAAGVVA"
BASE COUNT          795 a          439 c          522 g          942 t
ORIGIN      
        1 atgatgacgt attactctgg acaccagtga aaattttgat agtatactca ttaaaaacac
       61 ctatgttgtt caggtacaaa ctaacaaaca tcagaattat agaaaacagt aatgcagtaa
      121 agtatatatc atggtataat tcatatgatg aaaaaaaata gtgatatata aaaagaacaa
      181 taaaagtaac aacaattgat aatgacatgg aatgtgaaat gagcatagta ataagtaacg
      241 ctaatgaagc aataggtgct tcggcactgg taatgtaatc agcataacag ggattgagct
      301 gacaccatac ttgtaatcca agtgatacaa tgaatgcaat aataatgata tgaagtaaat
      361 gaaatacatg gaatagagca attacactgg tagcaataat cagcgcctgt attatagtaa
      421 tactattatt gcggatgtat gggttctgta gtagtttcat gggttgtggg caggtccttt
      481 tgttcttcct gatgttgtgg ttgaacagtt gcgggagttt cgcgttgttt ttgaacttct
      541 gtgtaaatgg gttttagcaa tttatcttct ttaactttat taataaaatt tgtgttgtag
      601 tagttactgg acagtatctc tttaactttt gaggtgtcat ttataaaagc gtagcatttg
      661 cagagtataa ataagtcatt gtctgataac ctgaatttat tgaaatgaac tatcgcttca
      721 ttgtattgag caagcatata cagtgagcgc gcataattca atgataacga ttcatcacga
      781 aggttttcag gtacaattgt ggtaagttct acaaccttgg aatattgctt tgttgtgtaa
      841 taatactgag ctgttataat gaatgcctta atatatttgg cagggatttc tttatgcttc
      901 tgtcgatatt gcatgtatac tttttcaaaa tattcatacg ctttattgtc atttttgttg
      961 taaaaaacaa gccctagctg gtacagtact tcagggttat tggagtcaat tttatatgca
     1021 gcctgcagtg tttgcagagc accatcataa tcattctttt gcatctggat ggctgatagc
     1081 aataaatatg gtgaaaggtt gccttttttt gtagatatgg cctgctgtaa aaaagtttcg
     1141 gcttttgtat actggccaat gttatagtat gccgtagcaa ctttaagctg gcttgagtat
     1201 ttcatatctt tatcaataaa cgcatattcc agtgctgcct caaatgctgt tgcagcttca
     1261 ctgtatcgtt tttcctgaaa atatatttcg cctacaagca actggagttc accgtaaaac
     1321 ggtatgcggt acaaaatctg gttgaaatat gacagcgcat cacggtactg gttttgcgtg
     1381 taataataca tgcctatctc aaataccggg gaaagaaaag cgctatcaag cataatggcc
     1441 tgtctgaaat gttgtaatgc taacgaataa tcctttgatt tactattgat cccctcctca
     1501 tattcttttt ttgcatcttc gttttcggtc cacatgagtt tattaatcag attttccgct
     1561 ttactttttg ctgatgcttc tcccagcacc tcataaaaat atttgcaggt tttaatggtt
     1621 ttgggaaaat cggatctgga ttcgtagatg acaataaggt tccagtatgc taaattacga
     1681 tattttggtg tcatcttgcg ttcaactgcc ttttcaaaat attccagagc agttgtaaga
     1741 ttcccattgt ttttttcaat ttcgccaaca aagtaatagg aatcgccccg gtcagttgtt
     1801 tcaatatcct ttaaaaaatt ttcttttgct ttttcccaat ttttatggac ataatagcga
     1861 caaccagctt catagtaact accagcacgt gcaggtgtag caaacgcaat aattacggat
     1921 aacaaaatgc aggaacatac agtatataca aaagcatgtt tcatgtcaaa tatccttgaa
     1981 atagatagtg ttatggcaat tttgtaaaat gttatgtaca gtatttacat gtacaactct
     2041 ggctacaatg aataatactt ctattcattt cttaatgatg tatgctaata attactgctg
     2101 atagttataa aatatgtcaa tacattaatt ttatatgaac tctgtcaggt caacaacaca
     2161 tatagcaata gcaacggcat tgagcaggct actgggcttt gtacgcgata tgcttattgc
     2221 ctggatgttt ggtgcatcct ggcagattga tgcattcttt gttgcattca gaatacctaa
     2281 tctggtgcga aggctggttg ctgaagggac attatcattg ccacttgtat ctgttattat
     2341 gagctatcgc cacaaagaag aaagaaaaat aatagctaat gatttatttt cattatacat
     2401 catgtcgggg tttctcattt ctgttacagg tattgttgca gcacctgcca ttgtaaaaat
     2461 tcttggtttt ggatttactg gaagcgtata tagtctttct gtcgtattgc tccgtatcat
     2521 gtttgtctat tgcctgtgtg caagtattgt tgcatatgcc atgggtgtgt taaacactga
     2581 aggagttttt tttatgcctg cattatcgcc actgttgctt aacgtgggca ttattctttt
     2641 ttgctttatt gcatatctgt attccagtgt catgtggctt gcagcgggtg ttgtggca
//
LOCUS       DAOSZP010000306         2697 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_29325_length_2697_cov_3.000000, whole genome shotgun sequence.
ACCESSION   DAOSZP010000306 DAOSZP010000000
VERSION     DAOSZP010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2697
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29325_length_2697_cov_3.000000"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            33..1229
                     /locus_tag="PK348_07175"
     CDS             33..1229
                     /locus_tag="PK348_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014042134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HXK66032.1"
                     /translation="MYVITGLIICLCLGSVYSYSVFLKPITESFGITATQGNLPFMFF
                     LAFFSILMFFSGKIMAKLGAKKLSIFGGIVVGLGWALSYLAVEAKSINLLILTYGIIA
                     GSGVGIVYGCPINIVGQWFPDKKGLATGLVLAGFGGSALITAKLAGALIPSVGLSTTF
                     LYFGIAFAILIVLLALPFRNPESGWKPAGWAPSAAAASAVDYDSSEMVKTSTFWGLFL
                     AYIIGCLAGLMAIGISKPVGSEIIKIDGETATTLVGIFAIFNALGRPLFGWLTDKITP
                     KGAAILNLLIIITVSIMMLMAKEGDVTLYVVAFCGFWLILGGWLAIAPTTTLAFYGLK
                     NYARNYGVVFFAYGLGAIIGGIISGQAKDVFGSYQVAFWPTLVLGVVGLMIVMAFLKP
                     PVKKAA"
     gene            complement(1275..2150)
                     /locus_tag="PK348_07180"
     CDS             complement(1275..2150)
                     /locus_tag="PK348_07180"
                     /inference="COORDINATES: protein motif:HMM:NF014046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ARMT1-like domain-containing protein"
                     /protein_id="HXK66033.1"
                     /translation="MKPTFHCIPCHVNHSYRVIQKLNLDHDSALRLMQELTSISIGSY
                     QTPPDCAYELYTTITKITGIHDPFSDIKKQSNMLMLQFLPQLRTLCTAGEPIRQALRI
                     ALAANVIDYGIGDTDAKIDYWEAFNTVMNDIYVPDDFYNEFIKAASSSQKILYIADNA
                     GEIVADRLLMEYLAVEKITCVVRGNPVINDATIEDAQSVGLNTSVTVITTGDRTPGIN
                     LDRCSKNFLHELSQADMVILKGQGNFETMIDAPLDGIVKKGLKMFFIFKVKCLPVAHY
                     IHRRLGDSAFILREI"
     gene            complement(2164..>2697)
                     /gene="pyrR"
                     /locus_tag="PK348_07185"
     CDS             complement(2164..>2697)
                     /gene="pyrR"
                     /locus_tag="PK348_07185"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF003549.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HXK66034.1"
                     /translation="EEKVLMNAKQIEEAINTISDGIIKEFGDVTNVALVGIQTRGVEI
                     AKRIKKRLEKNSGKEIKMGTLDITFYPDDLATRGMLPAIKETTITFNITKKIIILVDD
                     VLFTGRTIKAALETLMSFGRPQIIRLAILVDRGNRELPIQPDYCGLKIQTKIGDKVKV
                     RLKETDGIEDTVLYCTE"
BASE COUNT          741 a          526 c          518 g          912 t
ORIGIN      
        1 tatttggcct atcaccggaa gccggtcgat gggtgtatgt tattacaggc ttgataatat
       61 gcctgtgttt aggcagtgtg tattcataca gtgtattctt aaaacctatc actgaaagct
      121 ttggtattac tgccactcag ggaaatcttc cctttatgtt tttccttgca tttttctcaa
      181 ttcttatgtt tttctccggt aaaataatgg ctaaattagg ggcaaaaaag ctctctattt
      241 ttggtggcat tgttgttggg ttaggatggg cgctttcata ccttgctgta gaagcaaaaa
      301 gcattaacct tttaatatta acgtatggta tcattgcagg tagtggtgtt gggattgttt
      361 atggatgtcc tattaacatc gttgggcagt ggttccctga caaaaaaggt cttgctacag
      421 gcctggtact ggctggtttt ggcggctctg cgttgatcac cgctaaatta gcaggggctc
      481 tgataccttc agttggcctt tcaactacat tcctttattt tggtattgca tttgcaatac
      541 ttattgtact acttgcactt ccatttagaa atcctgaatc cggctggaaa cctgcaggat
      601 gggcaccatc agctgcagct gcaagtgcag ttgattatga ttcatctgaa atggtaaaaa
      661 catcaacttt ctggggtctt tttttagcat atatcatcgg ctgtttagca ggcttgatgg
      721 ctattggtat ttctaaacca gtaggcagtg aaataatcaa aattgatggc gagactgcaa
      781 caacccttgt tgggattttt gcaattttca acgcattggg tcgcccactt tttggatggc
      841 tcactgataa gattacacca aaaggtgcag caatacttaa cttgctgata atcataaccg
      901 tgtccataat gatgttgatg gcaaaagaag gtgatgtaac cctttacgta gtagctttct
      961 gtggattctg gttgatatta ggtgggtggc ttgcaattgc tccaacaaca acacttgctt
     1021 tttatggttt gaagaattat gcacgtaatt atggtgtagt attttttgca tacggccttg
     1081 gtgccattat aggtggcatc atatccgggc aggcaaaaga tgtgtttggt tcatatcagg
     1141 ttgctttctg gccaaccctt gttcttggtg tagtgggact catgatagtc atggcattcc
     1201 ttaagccacc agtaaaaaaa gcagcttaaa cactatatat atcactaaaa tataaagggc
     1261 acaaaatgtg ccctttatat ttctcgtagt ataaaagcac tatctcctaa acgcctgtga
     1321 atataatggg caacaggcag gcatttaact ttaaatataa aaaacatttt taagcctttt
     1381 ttcacaatcc catccagggg agcatctatc attgtttcaa aatttccctg tccctttaaa
     1441 attaccatat cagcctgcga tagctcatgt aaaaagtttt tgctgcaacg atcaagatta
     1501 atacccggag tacggtcacc ggttgtaatc actgtaacgg aagtattaag accaacagat
     1561 tgtgcatcct caattgttgc atcgtttatc acaggattac cgcgaaccac acaggtaatc
     1621 ttttccacag caagatattc cataagcagc ctgtcggcaa ctatctcccc tgcattatcg
     1681 gctatataaa gaatcttttg tgatgatgaa gccgctttga taaattcatt ataaaagtca
     1741 tcaggtacat atatatcatt catcaccgtg ttgaatgctt cccaataatc aatttttgca
     1801 tccgtatcac caatgccata atcaataaca ttggcagcca gtgctatgcg caatgcctgc
     1861 cttatgggct caccagcagt acataacgta cgcagttgag gcagaaattg tagcatcagc
     1921 atattgcttt gttttttaat atcgctaaag gggtcatgaa tacctgtaat ttttgtgata
     1981 gttgtatata attcatatgc acaatcaggt ggcgtttgat aactacctat gctaatggat
     2041 gtaagttctt gcattaatcg taatgcacta tcgtggtcta gatttagctt ttgtataaca
     2101 cgataactat gattcacatg gcaggggata caatgaaagg tgggtttcat attttacttt
     2161 gatttattca gtgcaatata atacagtatc ttcaatgcca tctgtttctt taagtcgcac
     2221 tttaacctta tcgcctattt ttgtctgtat cttcaaccca caataatcag gctgaatagg
     2281 aagctcacga ttaccacggt caacaagtat agctaagcgt attatttgag gtcttccaaa
     2341 tgacattaaa gtttccagag cagctttgat tgttcttccg gtaaaaagca catcatcaac
     2401 tagaataata atctttttgg taatattgaa tgttattgtt gtctctttta ttgccggaag
     2461 catcccacgt gttgctaaat catcaggata aaatgtaata tcaagtgtac ccatcttgat
     2521 ttctttaccg ctattttttt ctaagcgttt ctttattcgt ttagctatct ccacaccacg
     2581 ggtttgtatt ccaacaagcg ccacatttgt tacatcgcca aattctttga taataccatc
     2641 gctgatagta ttaattgctt cttcaatttg ctttgcattc atcaatactt tttcttc
//
LOCUS       DAOSZP010000307         2687 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_29449_length_2687_cov_3.246201, whole genome shotgun sequence.
ACCESSION   DAOSZP010000307 DAOSZP010000000
VERSION     DAOSZP010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2687
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29449_length_2687_cov_3.246201"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..159
                     /locus_tag="PK348_07190"
     CDS             1..159
                     /locus_tag="PK348_07190"
                     /inference="COORDINATES: protein motif:HMM:NF020130.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HXK66035.1"
                     /translation="MPFFSKYGNVASATLPLEMFLAKHENRLNRGDSIMFIVPAAGVQ
                     CGIMFFQY"
     gene            complement(211..1173)
                     /locus_tag="PK348_07195"
     CDS             complement(211..1173)
                     /locus_tag="PK348_07195"
                     /inference="COORDINATES: protein motif:HMM:NF013087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HXK66036.1"
                     /translation="MALTPTPVRTLQHYLEEAIFVGQAHRLGVFKELHSKPDTADGLA
                     LRMQFDKRATWILLEALVELGYLVKNNDRYMPTEYCVSHLVDEQSPDYEGFFLQFLLY
                     LINPWKTLPHVLRTGAPDETSFKELSMHDFIKGMDSPWKKKLAPEIVDLCIAQCSDPT
                     MCIDIGGAPGTIAKEFVRRGIATIVFDLPQSLEVTQDELRTIPTIEVIAGDATVALPD
                     CTVDIAFLGNLCHGQSPEDNQKIISMCHDILNSNGIIAIFDNLRNESYLGATLALHML
                     TQSKKGNIYSREEYFEWLYNAGFSKPRIYQLSDKAWQLIIAKKH"
     gene            complement(1228..1935)
                     /locus_tag="PK348_07200"
     CDS             complement(1228..1935)
                     /locus_tag="PK348_07200"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXK66037.1"
                     /translation="MLTVDFDLLAVQDGQNTLDAGCGLGRHSFEFVARGAHVFSMDMD
                     MDCVRRTRFTLVHMRDSGQSKPGARFQVLVGDALNLPFRNNTFDRIICSEVMEHVSDD
                     NRACSELVRVLKPGGLIAITVPTYISELLYDTLTYEYFTSPGGHIRKYVPRKLAQIME
                     QNGLKIYAIDFRHSFHTIYWLIRCVVGLHLENQFFTRHYRNFLTRVLFSPFMQKVERF
                     FDNFFPKSIILYAQKPL"
     gene            complement(1922..>2687)
                     /locus_tag="PK348_07205"
     CDS             complement(1922..>2687)
                     /locus_tag="PK348_07205"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HXK66038.1"
                     /translation="PSSFFLKTKRILYYPLLMQKYVANKMDLIITVSHDSAKEIYNAF
                     GVPVEKQRVVYNGMDSSIFYPIKMRKRPNSLIFVGNVEDRKKGVIYMLKALTLTKHKV
                     HLTIVDGGAPNRNYVPKWIDKFNLNDRITFTGKIPLDKLVQLYAQTQIALCPSLYEGF
                     GFPAAEAMACELPVIAATGGALPEVVGEHMKTGILVPPRDPYALAEAIDYLIENPSVR
                     ERIGKAARERVLKTFTWENAAKQMVTVYQEAIDAHR"
BASE COUNT          742 a          565 c          581 g          799 t
ORIGIN      
        1 atcccttttt tttcaaaata tgggaatgta gcttcagcta ccctgccatt agaaatgttt
       61 cttgcaaagc atgaaaatcg tctcaacagg ggtgattcaa ttatgtttat tgttcctgct
      121 gctggggttc agtgtggtat aatgtttttc caatattgat tgtaattaat gatagtatta
      181 ctgatattcc cgctgcctgt gcaagaataa tcaatgtttt tttgcgatta tcagttgcca
      241 tgctttatca gaaagctggt atatcctggg ttttgaaaaa cctgcattat aaagccattc
      301 aaaatattcc tcacgtgaat atatattccc ttttttactc tgtgtcagca tgtgcagggc
      361 aagggtagct cccagatagc tttcatttcg caaattgtca aaaatggcaa tgataccatt
      421 ggaattcaaa atgtcatggc acatgctgat aatcttttga ttatcttccg gtgattgacc
      481 gtggcacagg ttgcctaaaa atgctatgtc aactgtgcaa tcaggcaacg ccacggttgc
      541 atctccagca atgacttcta tagtgggaat tgtgcgtaat tcatcctgtg tcacttcaag
      601 ggattgggga agatcaaata ctattgttgc aattccccgg cgcacaaatt ctttagcaat
      661 ggttcctggc gcaccaccta tatcaataca cattgtgggg tcagagcatt gtgcaatgca
      721 cagatcaact atctcaggag ctaacttctt tttccatggc gaatccatac ccttgataaa
      781 atcgtgcatg gataattctt taaagcttgt ttcatcaggt gcaccggttc tcagtacatg
      841 gggaagagtt ttccatggat ttatgaggta cagtaaaaat tgtaaaaaaa agccttcata
      901 gtcgggactt tgttcatcaa caagatggct cacgcaatat tcagttggca tatacctgtc
      961 attgtttttt acaagatacc ctaattcaac aagcgcttca agcagtatcc atgttgcgcg
     1021 tttatcaaac tgcattcgta acgcaagtcc atctgcggtg tcgggcttgc tgtgaagttc
     1081 tttaaacacg ccaaggcggt gcgcctgtcc aacaaagatg gcttcttcta aataatgctg
     1141 caatgttcgc acgggtgttg gggtcagagc catatattct tccttttttc aaatatggat
     1201 ttttaccatg aactatcgcc catagtatta taatggtttt tgtgcataca gtatgatgct
     1261 ttttgggaaa aagttgtcaa aaaaacgttc aactttttgc ataaacggcg agaacagtac
     1321 acgcgttaaa aagttgcgat agtgcctggt gaaaaactgg ttttctaaat gaaggcccac
     1381 cacgcagcgg ataagccagt atatagtatg gaaagaatgg cgaaaatcaa tggcataaat
     1441 tttaagacca ttctgttcca tgatttgtgc cagtttgcgg ggaacatatt tgcgaatgtg
     1501 cccaccgggg gaggtgaagt attcatacgt cagcgtgtca tataaaagct cggatatata
     1561 cgttggtacg gttatggcaa taagcccgcc gggctttaat acacggacaa gttcactaca
     1621 ggctctgtta tcatcactga catgctccat aacctcggaa caaatgatgc ggtcaaaggt
     1681 attgttacgg aagggaagat tgagggcatc accaacaagc acctgaaaac gggcacctgg
     1741 ctttgactgg ccgctgtcgc gcatgtgaac taacgtaaac cgtgtacggc gaacacagtc
     1801 catatccata tccatggaaa agacatgtgc cccacgtgct acaaattcaa aggaatgccg
     1861 ccccaaacca caaccagcat caagtgtgtt ttgaccatcc tgcacagcca gaagatcaaa
     1921 gtcaacggtg agcatcaatt gcctcctggt acacggttac catctgtttg gctgcatttt
     1981 cccaggtaaa ggtttttaaa acgcgctctc ttgctgcttt gcctatgcgc tcccgcaccg
     2041 atggattttc aatgaggtag tctatggctt cagccagtgc gtatggatca cggggtggta
     2101 ccagtattcc tgttttcatg tgctcaccta caacctcggg cagcgcaccg ccagttgctg
     2161 caataacggg aagctcgcat gccatggctt cagctgctgg aaagccaaat ccttcatata
     2221 atgaaggaca cagagctatt tgagtttgtg catataactg tacaagttta tccagtggaa
     2281 tttttccagt gaatgtgatg cggtcattga ggttaaactt gtcaatccat ttgggcacat
     2341 aattgcggtt gggagcacca ccatcaacaa tggttaaatg aactttatgc tttgtcaatg
     2401 tcagggcttt cagcatgtag atgacacctt ttttacggtc ctctacattg cccacaaaga
     2461 ttaagctgtt tggccgctta cgcattttta tgggataaaa gattgagcta tccattccat
     2521 tgtacactac acgctgcttt tcaactggta caccaaatgc attgtaaatt tctttggccg
     2581 aatcatgtga tacagtaatg ataaggtcca ttttgtttgc tacatatttt tgcattaaaa
     2641 gcggatagta cagaatgcgc ttggttttaa gaaaaaagct tgatggg
//
LOCUS       DAOSZP010000308         2685 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_29477_length_2685_cov_3.703422, whole genome shotgun sequence.
ACCESSION   DAOSZP010000308 DAOSZP010000000
VERSION     DAOSZP010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2685
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29477_length_2685_cov_3.703422"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1549)
                     /gene="glgX"
                     /locus_tag="PK348_07210"
     CDS             complement(<1..1549)
                     /gene="glgX"
                     /locus_tag="PK348_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020161016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HXK66039.1"
                     /translation="MQTVKHHHFVTLPGAPLPLGVHLDRHGARFALFSRHATAVSILL
                     FASSETEEYIEIELDPKLNKTGDIWHIWIAGIREGQLYGYKVDGPYNPLEGHRFNKNK
                     LLLDPYAKAISGNHIWDFSKACGYIKGHPDEDLSFSTDKNYKESPKCIVVDTEFDWYD
                     RPIQTPMEDTIIYELHVKGFTFHPSSQVTHRGTYKGLSEKIPYLKQLGITAVELMPIQ
                     EFDEFENININPLTGERLKNYWGYSTIAFFAPKSTYSSSGTMGQQINEFKEMIRDFHE
                     AGIEVFLDVVFNHTAEGDHLGPTIHFKGLDNSIYYMLKENKRFYQNFSGCGNTFNCNH
                     PLVRDFILDCLRYWVVDMHVDGFRFDLASILGRDQDGNILQNPPLIEKISEDPILRNT
                     KIIAEAWDAGGAYLIGHFPGRWAEWNGKFRDDVRRFWRGDNDTVFNFMQRIIGSPDIY
                     QRTNRSPLHSINFITCHDGFTMLDLVSFNEKHNLANGENNQDGENNNLSCNYGFEGLD
                     APPDIINI"
     gene            2229..>2685
                     /locus_tag="PK348_07215"
     CDS             2229..>2685
                     /locus_tag="PK348_07215"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF015727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HXK66040.1"
                     /translation="MIKIAELLPPRPSSLWKMVKQCGVEYVVGGMQLYSGWESMPKDF
                     WPWSYNSLVHVKTTYEDAGFKLEVIESRPPMEKIKLGLPGRDEEIENIIMLIQNMGKL
                     DIPVWCYEWMPVFGWTRTSTTTPVRGGALATSYDHSFMEKAPLTEYGIIT"
BASE COUNT          823 a          483 c          538 g          841 t
ORIGIN      
        1 gaatgtttat gatgtctggt ggggcatcta acccttcaaa gccgtaatta cagcttaagt
       61 tgttattttc gccatcctga ttgttttcac cattggccag gttatgcttt tcgttaaagg
      121 atactaaatc aagcatggtg aatccgtcat ggcaggtaat gaagttaatg ctgtgcagcg
      181 ggctacgatt tgtccgctgg taaatgtcag ggcttccaat aattcgctgc ataaaattga
      241 aaacggtatc attatcaccc cgccagaaac ggcgcacatc atcgcggaat ttcccattcc
      301 attcagccca tcttcccggg aaatggccaa ttaagtatgc cccgcctgca tcccaggctt
      361 cagcaattat tttggtattg cgcaatatgg gatcttcaga tattttttct atcaaaggtg
      421 ggttttgtaa gatattgcca tcctggtcac gacctaatat tgatgcaagg tcaaacctga
      481 agccatcaac atgcatgtcc acaacccagt aacgcaggca atcaaggatg aagtcgcgca
      541 ccagcgggtg gttacagtta aacgtattgc cgcatcctga aaagttctgg taaaaacgct
      601 tgttttcctt gagcatgtag taaatggaat tatctaagcc cttaaaatgt atagttggtc
      661 ccaggtggtc accctcggcg gtgtgattaa aaacaacatc aagaaacact tcaatacccg
      721 cttcatggaa atcacggatc atttctttaa attcattaat ctgctgtccc attgtgccac
      781 tggatgaata cgttgatttg ggtgcaaaaa aagcaatggt gctgtatccc cagtagtttt
      841 tcagccgctc accggttaat gggtttatat tgatattttc aaattcatca aattcctgaa
      901 tgggcataag ttctacagca gttataccta attgcttaag atatgggatt ttttcagaaa
      961 gacctttgta tgttcctcta tgggtaacct gtgatgaagg atggaaagtg aaccctttaa
     1021 catggagttc ataaataata gtatcttcca tgggagtctg aatggggcga tcataccagt
     1081 caaattctgt atcaacaaca atgcattttg gtgattcctt atagttcttg tcagtggaaa
     1141 aagaaaggtc ttcatccgga tgccctttta tatatccaca tgctttactg aaatcccata
     1201 tatgattgcc gctgatggct tttgcatagg ggtctaaaag gagcttgttt ttattaaaac
     1261 ggtgaccttc cagaggatta taaggcccat caaccttata tccgtacaac tgtccttccc
     1321 gtattcctgc aatccatata tgccatatgt cgccggtttt attgagcttt gggtcaagtt
     1381 caatttcaat gtattcttcg gtttctgaag aagcaaaaag cagtattgat acagcagtag
     1441 cgtgtcggct gaaaagtgca aaccgtgcac catgtctgtc cagatgtaca cctaacggga
     1501 gtggtgctcc cggcaacgta acaaaatgat gatgttttac agtttgcata gtagttagca
     1561 ataattgtct tttagtaatt ttcaatttat tattctaagt atgatacata taagcgtcaa
     1621 ttaaatttat tattgtaaca aaagcattct ggaaaatata tttgactgtc tgataatgaa
     1681 ttactgtgtg gttcaaccat ctacttttga tttctttact cttaaagatc atttgccggc
     1741 aatatttata gcagctcaaa ttttaaagta tgctaaaaac aatttcttta acaaaacgtg
     1801 aagcgggtga ttttgttttt ttcaggccaa agcaggggga tgctttaatg caattaaggg
     1861 acatgatgat aagacatgga aatataattg tatcctggaa acctatccct gaaagatttt
     1921 cattgcacgt acatttacat taataaagat tatacagggt aaatgaagtc cattcacaat
     1981 taactccttg atacccatat ataaatatat atgacataat catttgtata aaaaaaagaa
     2041 atacataaat aattttttta aaaaatgttg acaaattggg tatattaaca taacatcaga
     2101 ccatcagata ttcagattta tacctggtat aactggatta gaaatatttg tactattcat
     2161 gattgttaat gttacagatt gatgataaca aaagtaaatt ttaaattttt taaggaagta
     2221 aacagtatat gattaaaatt gctgaacttc tgccacctcg tccttcttcg ctatggaaga
     2281 tggtaaaaca gtgtggtgtt gagtatgttg taggtggcat gcaactgtat tcaggatggg
     2341 aaagtatgcc caaagatttc tggccatgga gctataattc tttggtgcat gttaaaacta
     2401 cgtatgaaga tgctggattt aaactggaag tcattgagtc aaggcctcca atggaaaaaa
     2461 taaaattggg attaccaggc agggatgaag aaattgaaaa tataataatg ctgatacaga
     2521 atatgggcaa acttgatatt cctgtgtggt gttatgagtg gatgcctgta tttggatgga
     2581 caagaacctc aacaaccaca cctgttcgtg gcggagcgct ggcaacatcg tatgatcact
     2641 catttatgga aaaagcgcca ctgactgagt atggcattat tactg
//
LOCUS       DAOSZP010000309         2678 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_29572_length_2678_cov_3.244377, whole genome shotgun sequence.
ACCESSION   DAOSZP010000309 DAOSZP010000000
VERSION     DAOSZP010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2678
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29572_length_2678_cov_3.244377"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1800)
                     /gene="hypF"
                     /locus_tag="PK348_07220"
     CDS             complement(<1..1800)
                     /gene="hypF"
                     /locus_tag="PK348_07220"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HXK66041.1"
                     /translation="MTHHYHIQVKGIVQGVGFRPFVYNVAVSHNLCGSVSNDTEGVTI
                     HLEGPDEAIKQCVDIIRNNPPPLAQIIDIKITKLPPNGFKSFTILQSNTTGTRDVFIP
                     PDVAICHECLEDFFDVHNRRYHYPFTTCTHCGPRFSIIDDIPYDRDNTAMKPFSMCPQ
                     CYEEYTNPHNRRFHTQPNACPQCGPHIVLCDSSGKVIQSQFDAIISQLQTILKHHIVA
                     LKSVGGYHLACDATNDHCVNLLRMRKGRPFKPFAIMVSSLQFLETFCLVTQKEKELLL
                     SKERPIVLLKLQKDIVSPYVAPGLTYIGVMLPYTPFQHMLFSSNDESIYVMTSANISD
                     EPIIYNDDDAFERLGTIADYFVTYNRQILSHTDDSVVYVVDEKPYFVRRSRGYVPAPI
                     FIKETPLHILATGGDLKNSFALARNNVAIVSQFLGDLATPWGNDLYRKTIAHFCKIFD
                     VKPKLIAHDMHPNYFTTMFAQEQTTLQTVAVQHHHAHIASVCQEHSIEEPVIGIAYDG
                     TGYGTDHTLWGSELLIADTKEFIRAAHFDYFGLPGGEHAIKDPWKIGLSLLMTSSVDD
                     STFFTQKQEKEFVKEIVYKTINCPATCSIGRLFD"
     gene            2276..>2678
                     /locus_tag="PK348_07225"
     CDS             2276..>2678
                     /locus_tag="PK348_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014323307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HXK66042.1"
                     /translation="MSKIRLFVVCLGILVIAVIIGCSEGTTNINPSPTDVVTVTDINK
                     FITEKDKLAGIAATMNAMDPVAMPHKKHEDAGIKCVVCHHKAGNDDRIKQCAECHKGA
                     KGEDTIHGLCINCHAQSKKGPVLCNECHIAKE"
BASE COUNT          792 a          479 c          607 g          800 t
ORIGIN      
        1 gtcaaaaagc cttccaatgc tgcaggttgc agggcagttt atggttttat aaactatctc
       61 ctttacaaat tctttttcct gtttttgtgt aaaaaatgtg gaatcatcaa cagatgatgt
      121 cataagcaaa gacagaccta tcttccatgg gtcttttatg gcatgctcac cgccaggcaa
      181 accaaagtaa tcaaaatggg ctgcacggat aaactccttt gtgtctgcaa ttaacagttc
      241 acttccccac agtgtatggt cggttccgta tccagtgccg tcataggcaa tgccaataac
      301 aggctcttct atgctatgtt cctggcacac tgaagcaatg tgcgcgtgat gatgctgtac
      361 tgcaactgtt tgtagtgtgg tttgctcctg cgcaaacatg gtggtgaagt aattgggatg
      421 catgtcgtgc gcaatgagtt ttggcttaac atcaaagatt ttacaaaaat gggcgatagt
      481 ttttcggtaa aggtcattgc cccacggagt ggcaaggtcc cccagaaact gacttacgat
      541 agcaacgttg tttcgtgcca gcgcaaagct gttttttaaa tcaccgcctg ttgcaaggat
      601 atgaagcggt gtttctttga tgaagatagg tgcagggaca tatccccgtg aacggcgaac
      661 aaaatatggc ttttcgtcaa caacgtatac tactgagtca tcggtgtgcg atagtatctg
      721 gcgattgtag gtgacaaagt aatcggcaat tgtgcccaaa cgctcaaagg catcatcgtc
      781 gttgtatata atgggctcat cggatatatt ggcactggtc atgacgtata tgctttcatc
      841 attgctggaa aaaagcatat gttgaaatgg tgtataggga agcatgacgc caatataggt
      901 gagtcctggt gcaacgtatg ggcttactat atctttttgt aatttcagca gtacaatagg
      961 gcgctccttg cttaacaaaa gttctttttc tttttgagtc acaaggcaaa aggtttctaa
     1021 aaattgtagt gatgatacca tgatggcaaa gggtttaaat gggcgccctt ttcgcatccg
     1081 taaaagatta acacagtgat cattggtggc atcgcacgct aagtgatatc ccccaacgct
     1141 tttcagtgca acaatgtggt gttttaagat cgtctgcaac tggctgatta tggcatcaaa
     1201 ttgagattga ataaccttac cggaactatc gcacagcact atatgagggc cacactgtgg
     1261 gcaggcattg ggctgggtgt gaaacctgcg attgtggggg tttgtatatt cttcataaca
     1321 ctgaggacac atggaaaatg gcttcatggc ggtgttatcc cggtcatagg ggatatcgtc
     1381 aataatgctg aagcggggac cacagtgcgt gcaggtagta aacgggtaat gatagcgacg
     1441 gttatgcacg tcaaaaaagt cttccagaca ctcatgacag atagccacat caggtggtat
     1501 aaatacatcg cgtgtgcctg ttgtgttgct ttgcagtata gtaaaacttt taaatccatt
     1561 gggtggaagt ttggtgattt tgatatcaat aatctgagca agtggaggag ggttgtttcg
     1621 aataatgtca acgcattgct tgatagcttc gtcaggtcct tctaaatgaa ttgttacacc
     1681 ttctgtgtca tttgaaacgc taccgcaaag attgtggcta acagcgacat tatacacaaa
     1741 tggcctgaat cccactccct gaacaatgcc ttttacctgt atgtgataat gatgtgtcat
     1801 actatgcatt gtagcacaaa atattcatag cctgcaattc atacgtaaaa aattgatcag
     1861 caaaaacctg tgggcatatg aacaacgtga tcctgtggtt attccaataa aaaacatggt
     1921 ctttccaggg aagaattttg atgtatgcaa aattgctttg ttaatctact tatttcagtg
     1981 acgttgtgca cttcactata gtttgatagg acaaaggaag tcaataattt tatgtaatac
     2041 taaaatatta ttgacattaa tttctatatg ttatatgata gcacaattga taaagtaagt
     2101 acctgcaggt aatggatgat taatggagca tctggaatgt tattttaaaa aattaataaa
     2161 tcaattattt ggttgacttg caacgcataa taataaaatt tatattttta atggttttta
     2221 tgcctaattt ataacatgta acatgataaa tctatacaac gtaaggaggt aaaatgtgag
     2281 taaaattagg ttgtttgtgg tttgtttggg tatacttgtc attgcagtaa ttattggttg
     2341 ctctgaggga acaaccaata tcaatcccag cccaactgat gttgttacgg taacagatat
     2401 taataagttt attactgaaa aggacaaact ggcagggatt gctgcaacca tgaatgcaat
     2461 ggatcctgtt gcaatgccac ataaaaagca cgaagatgca ggtatcaagt gtgtagtgtg
     2521 ccatcataaa gctggcaatg atgacaggat aaaacagtgt gcggaatgcc acaaaggtgc
     2581 aaagggtgag gataccattc atggtctgtg cattaattgc catgctcagt ccaaaaaagg
     2641 ccccgtgctt tgtaatgagt gtcatattgc taaagaat
//
LOCUS       DAOSZP010000310         2665 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_29760_length_2665_cov_2.401533, whole genome shotgun sequence.
ACCESSION   DAOSZP010000310 DAOSZP010000000
VERSION     DAOSZP010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2665
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29760_length_2665_cov_2.401533"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..771)
                     /locus_tag="PK348_07230"
     CDS             complement(<1..771)
                     /locus_tag="PK348_07230"
                     /inference="COORDINATES: protein motif:HMM:NF013112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HXK66043.1"
                     /translation="MFSPDDISYYIISLGCPKNEVDSERLNADMHSAGFVEAPDIEKA
                     DIIFINTCGFIEAAKKESVETILDALDYTSSQTKTFKKRLVVLGCLTQRYKQEIIQDI
                     PEIDLVWGLYDSSLLKEIARLFNIALNAGTIAQKRKPLIDNLPYAYIKIAEGCSNFCS
                     YCAIPLIRGRFAAFPPHDIMKDAREALNRGVKELIIVAQDTAVYQYDDKRIWDILAMI
                     SNLEGDFWIRLMYCHPDHIDDRLIEALVTVPKVVPYIDI"
     gene            complement(773..1906)
                     /locus_tag="PK348_07235"
     CDS             complement(773..1906)
                     /locus_tag="PK348_07235"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4115 domain-containing protein"
                     /protein_id="HXK66044.1"
                     /translation="MVAGIGETLRNAREAKRLTLKDVSKDTNIVVKYLEALENEDFDK
                     FPSETYLLGFLRSYAEYLRVDADELIQAYKGYKIGESSTPLEELTRPTGLSIVAQLRM
                     YAQQYRNILYFAGIGIAVLIVISLFISLITSDVHVGSDDSIKAIQEEYYRTQGKKIAI
                     KNIWPLQLTNDSGIVLLYNEEACQFLVDNKEILFLLNDIKDNSVIVEFLPDSKKYVLE
                     MEKPVICSPMESSRNIVLTLKGLTENRAKIRVQLAAVEQEKPDEAVTVTTTVTPPAEV
                     VTHDQKNLKIVFEAEFVQKTYFELYLDGNERVRGMMPAGTKERWEASEFIQVKIGNAG
                     GVKVKINGTDYMFGLPGQVANKVITWRKDPQDPNKYKLEVKDW"
     gene            complement(1910..2605)
                     /locus_tag="PK348_07240"
     CDS             complement(1910..2605)
                     /locus_tag="PK348_07240"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer-membrane lipoprotein carrier protein LolA"
                     /protein_id="HXK66045.1"
                     /translation="MLSTLNKKVLCMVIAPVIVLLCVMTGYSYKFDFVTVNDIVKQIK
                     KTFSDVESYQANFVMISDRMGKRQNQSGTIWYMAPHYLRIDFDVPYGQKIVSDGKQVW
                     IYIPSINVVAEQDLNSSMFAIGTGSGLNRLFSKYHYKFDGKEQPRVEGNNKYYVLFLK
                     QKETRSGFRTMKLWVNEQYFITKAAGETANGKNITIKIKNINTKVDLKKGFFKFDPPS
                     NARIIKNPMLAEE"
BASE COUNT          793 a          573 c          407 g          892 t
ORIGIN      
        1 aatatcaata tacggcacaa cctttggtac cgtaacaagt gcttcaataa gcctgtcgtc
       61 aatatgatca ggatggcaat acataaggcg tatccagaaa tcaccctcaa ggttactaat
      121 catagccagt atatcccaga ttcttttatc atcatactga tatactgcag tatcctgcgc
      181 cacaataatt aattctttaa caccacgatt cagcgcttca cgagcatcct tcataatgtc
      241 atgtggtgga aaagcagcaa acctaccccg tatcagggga atagcgcaat agctgcaaaa
      301 attggaacac ccttctgcta tttttatata tgcataggga agattatcaa taagcggttt
      361 tcttttttgt gcgatagttc ctgcattcag tgcaatattg aataagcggg caatttcttt
      421 taataaagaa ctatcgtaca aaccccatac cagatctatt tcaggtatgt cctgtattat
      481 ttcctgttta taccgctggg tgagacagcc taacacaacc aatctttttt taaaagtctt
      541 tgtttgcgat gatgtataat ctaatgcatc gagtatagtt tcaactgatt ctttctttgc
      601 ggcttcaata aatccacagg tattgataaa tattatatct gctttttcta tgtcaggtgc
      661 ctcaacaaaa ccagcgctgt gcatatcagc attaaggcgc tcagaatcca cctcattttt
      721 agggcaccct aatgaaataa tatagtatga aatgtcatca ggtgaaaaca catcaccagt
      781 cttttacttc aagcttgtat ttgttgggat cctgtgggtc tttacgccag gtaattactt
      841 tattggcaac ctgcccaggc aggccaaaca tgtagtcagt gccattaatt ttaaccttta
      901 ctccaccagc attgcctatc ttaacctgaa taaattctga tgcttcccac cgctcttttg
      961 tgccagctgg catcattccc ctgacgcgct cattgccatc taaatacagt tcaaaatatg
     1021 ttttctgcac aaactcagct tcaaatacta tttttaaatt tttctgatca tgcgtaacca
     1081 cttcagctgg tggtgtaact gtggtagtca cagtgacagc ttcatcaggt ttttcctgct
     1141 caacagcagc caattgcact ctgatttttg ccctgttttc agttaatcct tttaacgtca
     1201 acacaatatt cctggaggat tccattgggc tgcatataac aggcttttcc atttccagaa
     1261 catatttttt tgaatcaggc aaaaactcta ctatcactga attatcttta atatcattta
     1321 ataaaaataa tatctcttta ttatctacca gaaactggca tgcttcttca ttatataaca
     1381 acactatgcc actatcattg gtgagctgta acggccaaat atttttaatg gcaatctttt
     1441 tgccctgagt acggtaatat tcttcctgga ttgctttaat tgaatcatcg cttcccacgt
     1501 gcacatccga agtaataagg cttataaaca gcgatattac aataaggaca gcaataccaa
     1561 tacctgcaaa atataaaata ttacgatact gctgcgcata catccgtaac tgagcaacta
     1621 ttgataaacc agtgggcctg gttaactctt caagtggtgt tgaactttcg ccaatcttat
     1681 atcctttata tgcctgaatc aactcatcag catcaacacg aaggtattca gcatatgaac
     1741 gtaaaaaacc cagaaggtat gtttcgcttg ggaatttgtc aaaatcttca ttttcaagtg
     1801 cttccagata cttaacaaca atatttgtat ctttggatac atcttttaag gttaaacgtt
     1861 tagcttcacg tgcattgcgt aatgtttcac caattcctgc aaccatatat tattcctctg
     1921 ccaacattgg atttttaatt attctggcgt ttgaaggagg atcaaattta aaaaaacctt
     1981 tttttaaatc tactttagta tttatatttt tgattttaat tgtaatgttt ttgccatttg
     2041 ctgtttcacc agcagctttt gttataaaat actgttcgtt aacccacagc ttcatagttc
     2101 tgaagccact tcgtgtttcc ttttgcttta agaataacac ataatattta ttgttacctt
     2161 ctacacgtgg ttgttctttg ccatcaaatt tataatgata ttttgaaaaa agcctgttta
     2221 aacctgaacc agtaccaatt gcaaacattg aactgtttaa atcctgctca gcaaccacat
     2281 ttattgaagg aatataaatc cacacctgct tgccatctga aacaattttc tgaccatatg
     2341 gcacatcaaa atctatcctc aggtaatgtg gtgccatata ccatatagta ccactctgat
     2401 tctgccgctt tcccatcctg tccgaaatca tcacaaaatt tgcctggtag ctttcaacat
     2461 cagagaatgt cttctttatt tgcttgacta tatcattgac agtcacaaag tcaaatttat
     2521 aggaatagcc tgtcattaca cacagtaata caatgaccgg agctatcacc atgcataata
     2581 cttttttgtt aagagtgctc aacatgatgc cctttataaa tttttattta acccaatgtg
     2641 catatacatt gttgcaaaca acatt
//
LOCUS       DAOSZP010000311         2664 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_29772_length_2664_cov_3.225757, whole genome shotgun sequence.
ACCESSION   DAOSZP010000311 DAOSZP010000000
VERSION     DAOSZP010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2664
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29772_length_2664_cov_3.225757"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..566)
                     /locus_tag="PK348_07245"
     CDS             complement(<1..566)
                     /locus_tag="PK348_07245"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HXK66046.1"
                     /translation="MVSWMSANDIRFIIVLLIFHMGCVPPAYRQPQDIHKKNTQKTYQ
                     QFNYNEDDSTPLITDEDNYYIKNDYYSTQPVYYTTPQVESETSYHNSQSNENNNSKQS
                     SSKGTWHIIKKGETLYRLSKQYNTTVDAICEANNINNFTTIKVGQKIFIPSNDFVNNS
                     SSKKSSPNQIPQKKFDSSFSFIWPVPFV"
     gene            complement(673..1092)
                     /gene="nikR"
                     /locus_tag="PK348_07250"
     CDS             complement(673..1092)
                     /gene="nikR"
                     /locus_tag="PK348_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HXK66047.1"
                     /translation="MNNVIRFGVSIDEKLLDRFDALINEKGYVNRSEAIRDLIRDMLV
                     KEEITDPNAEVIGTLTLVYSHDVREIADKLNDLQHDNYKYIISSVHVHLDEHNCLEVL
                     LLRGTSSQVKKIADNLLAIKNVRHGKLTITTTVDYHE"
     gene            1466..1861
                     /locus_tag="PK348_07255"
     CDS             1466..1861
                     /locus_tag="PK348_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013259983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66048.1"
                     /translation="MPVFSNTEKMYEVLGELFNALLDDTTAREKFLESDIIIKFDIHD
                     PAGQIWISNEDGGKVICGSTQLKPTIEMTLSGDSCHKFWLQELTMPVALAKGLIKAKG
                     PMPKVIKLLPLLKPAYAAYPEIAKKHGLI"
     gene            1874..2551
                     /locus_tag="PK348_07260"
     CDS             1874..2551
                     /locus_tag="PK348_07260"
                     /inference="COORDINATES: protein motif:HMM:NF021439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2148 domain-containing protein"
                     /protein_id="HXK66049.1"
                     /translation="MSIERSMKQFEEDRKAAVKIAAHLLLASATTTPRVGGVGECTVH
                     IIDDECKIEDLCQAIEKMAKENERWEFFTRDAMMLRDADAVLIITSLRCYTDPADTNC
                     NMCGKLTCEYLKESEKLPDDPGIAYKGPLCIFRANNIAYAVDGIVSLARNLGIDYGVF
                     WSAGAAAMRMGIVPKNTGFCLAVGISVTEKSPFRDIPLRYDEINEKTMNDRIIKRLWP
                     QFRSIYS"
BASE COUNT          845 a          413 c          543 g          863 t
ORIGIN      
        1 ataacaaatg gtactggcca gataaaagaa aagctgctgt caaatttttt ttgtggtatt
       61 tggtttggag atgatttctt tgatgaagaa ttattaacaa aatcatttga aggaataaaa
      121 attttttgcc caacctttat tgtggtgaag ttgttaatat tgttggcttc gcagatcgca
      181 tcaacagtag tattgtactg ttttgaaagt cggtatagtg tttctccttt tttaatgatg
      241 tgccatgtgc cttttgaaga cgattgttta ctattattgt tttcgtttga ctgactattg
      301 tgataggagg tttcgctttc aacctgaggt gttgtataat acactggttg tgttgaatag
      361 tagtcatttt tgatgtaata attatcttcg tcggtgatta gaggggtact atcgtcttcg
      421 ttgtaattga actgctggta tgttttttgg gtgttttttt tgtgtatatc ctggggttga
      481 cggtatgcag gaggaacaca tcccatatgg aatattaata aaactattat aaaacgtatg
      541 tcattggcac tcatccatga aaccatggaa catctcctta tgttccgttt aaacgcacat
      601 ttaccagaaa gggtataaat acccttatca tagtatcgga agaatgggct ggatagttaa
      661 gcaattaatt ttttattcat gataatcaac agtggtggtg atagttaatt tgccatggcg
      721 tacgtttttt atggccagta aattatctgc aatcttttta acctgtgacg atgtgccccg
      781 taacaataac acctcaaggc aattatgttc atctaaatga acatggactg atgatattat
      841 gtatttgtaa ttatcatgct gaaggtcatt cagtttatcg gcaatctcac gaacatcatg
      901 gctgtataca agagtcaagg tgcctattac ttctgcattt gggtcagtga tttcttcttt
      961 aaccagcata tctcgaataa ggtcacgtat ggcttcagaa cggttgacgt atcccttttc
     1021 gttaatcaag gcatcaaacc tgtccagcag cttctcatct atagacacgc caaaacgaat
     1081 tacattattc atgctctgac ctcatctggt atatagtatg aatcaatagt gttgtagctt
     1141 tttacaatat gcaagtaaat tttttatctt tttctgcagt ataaacaata tcaagtattg
     1201 aaattgttat gataggtata acattataat agataacagg tttgatacgt gggaatgaaa
     1261 ataatagaaa aattgaattg acaaaattaa cattggttgt acaatcaatc aactataatg
     1321 attttatttt taaaggaggc agttatagat aaacataata tatagtataa attaaaaccg
     1381 atatattctt attatacttc tcccatacat gtccacattc agcactcaaa cataaaatta
     1441 taaataacta aaatggaggt tagccatgcc tgttttttca aacactgaaa aaatgtatga
     1501 agtattgggt gagctgttta atgcattatt agatgataca acagccaggg aaaaattttt
     1561 agaatcagac atcattatta agtttgatat tcatgatcct gcagggcaga tatggatatc
     1621 aaatgaagat ggtggcaagg ttatatgtgg ctcaacacag ttaaaaccaa caatcgaaat
     1681 gacgctttca ggtgattcat gccacaaatt ctggctacag gaactaacaa tgcctgttgc
     1741 attggccaaa gggttaatta aggcaaaagg acccatgcca aaagttatta aactccttcc
     1801 acttcttaaa cctgcatatg cagcatatcc tgaaattgct aaaaaacatg ggctgatata
     1861 aagggggtaa cagatgagta ttgaacgaag catgaagcaa tttgaggaag atagaaaagc
     1921 agccgttaag attgctgcac acttattact ggcaagtgca accaccacac cacgtgtagg
     1981 aggtgtaggt gagtgtactg ttcacataat tgatgatgaa tgtaaaattg aggatttatg
     2041 ccaggcaata gaaaagatgg caaaggaaaa tgaacgctgg gaatttttta cccgtgatgc
     2101 catgatgctc agagatgcag atgctgtgtt gattattaca tcgctacgat gttataccga
     2161 tcctgcagat actaactgca atatgtgtgg gaaactcacc tgtgaatatt taaaagaaag
     2221 tgaaaaattg cctgatgatc cgggtattgc ctataaaggt ccgttatgta tattcagagc
     2281 taataatatt gcttatgctg ttgatgggat agtgtcactg gctcggaatt taggcattga
     2341 ttatggagtg ttctggtcag cgggtgcagc agctatgcgt atgggtatag tgccaaagaa
     2401 tacggggttt tgtttggctg ttggtatttc agtaacagaa aagagcccat tcagggatat
     2461 cccgttgcga tatgatgaga taaatgaaaa aactatgaat gacaggatca tcaagcgatt
     2521 gtggcctcag ttcagatcta tttacagtta ataatgcacc acaacttcat tgattttgcc
     2581 cataacagat tgcatcatgt aatatataac aaagttattg gaggttttta attcctgcaa
     2641 tagaaatgga tgagcttata caga
//
LOCUS       DAOSZP010000312         2663 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_29785_length_2663_cov_2.872699, whole genome shotgun sequence.
ACCESSION   DAOSZP010000312 DAOSZP010000000
VERSION     DAOSZP010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2663)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2663)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2663
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29785_length_2663_cov_2.872699"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1003)
                     /locus_tag="PK348_07265"
     CDS             complement(<1..1003)
                     /locus_tag="PK348_07265"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HXK66050.1"
                     /translation="MFVDVAVGFPVDGFFTYSTDDTSITKGMRVVVNFNNRKTTGYVV
                     HVHDDPPNFDVKPVIKVLDTQPIFDDRLLRLAQFVSNHYVCYFGEALGTALPSGKSYN
                     IRTKPFTFGDSSKEVILTEEQEQIYRAIMNQPQKVHCIYGITGSGKTEVYIALAKKMI
                     QQGKAVLYLVPEISISSQMFERLKAVFGQTLVMYHSGMNPNQRLVSWKRFYTGQAMIA
                     VGTRSAIFMQAPNLGLIIIDEEHDSSYKENSTPRYHARTVAWYRHRQEGAMLVLGSAT
                     PSLETLYAIQRTSIVQHQLNKRFGTAKLPELEIVTVKASNARTIFSNTLLFSIKKAIE
                     "
     gene            complement(1006..1638)
                     /locus_tag="PK348_07270"
     CDS             complement(1006..1638)
                     /locus_tag="PK348_07270"
                     /inference="COORDINATES: protein motif:HMM:NF015146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HXK66051.1"
                     /translation="MYHLLKEAIVTHIQYGRRTPLVLAYTDEISYFFNRKNVAAPVEL
                     NTFQEKVQHAVAQCNKCKTTEKNIIHGGDASSGIMIILNPPSMLSSVEKKVLKPDVDT
                     MLDKMMKAIGVHVKTCYITHMIKCESQRELPGTMFSHCQNLLAKEIELVKPQIIIVMG
                     EMRPLQKIVKNSSGIQWFNIEHPITLLKNPELKKPAWNTLKLVKTVLDGK"
     gene            complement(1689..>2663)
                     /locus_tag="PK348_07275"
     CDS             complement(1689..>2663)
                     /locus_tag="PK348_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002771903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HXK66052.1"
                     /translation="LLKKNITLEDEMIGNSPPMQEVKRIIDTAASTNARVFITGENGT
                     GKELVARAIYRKSKRSDKPFVKVNCAAIPDELIESELFGHEKGSFTGAVARRLGKFEL
                     ADKGTIFLDEICDMSPSAQAKVLRVLQEQQFERVGGNEVITVDVRVIAATNVDVKEAI
                     EQGRFREDLYYRLNVIPIYVPPLSERREDIPLLVDYFLEKFAREHGLGVKQMNDSAME
                     FLVNYSWPGNVRELKNVIERLTIMVPSEVIQVQDVTKHIESYDYEDTIAKETSSLKKA
                     REQFEKDYIIKALKQYDKNISATAKALGIERTNLHRKIRQYHINIDRL"
BASE COUNT          733 a          590 c          494 g          846 t
ORIGIN      
        1 cttcaattgc ctttttaatt gaaaacagca atgtatttga aaatatagtg cgtgcatttg
       61 atgcctttac agttactatc tccagttcag gaagctttgc agttccaaaa cgtttgttca
      121 actggtgctg cacaatactg gtacgctgta ttgcatacag tgtttcaagt gacggtgttg
      181 ctgaacccaa taccagcatt gcaccttcct gccggtgcct gtaccaggca accgtgcgtg
      241 catggtagcg cggtgtgctg ttttctttat atgaactgtc atgctcctca tctataataa
      301 taaggcctaa attaggggcc tgcataaaga ttgcagagcg tgtgccaaca gcaatcattg
      361 cctggccagt ataaaagcgc ttccagctta caagccgctg gtttggattc attccgctat
      421 ggtacatcac caatgtctga ccaaacacag ctttaaggcg ctcaaacatc tgtgaggaaa
      481 tagaaatctc aggcaccaaa tacaaaacag cttttccctg ctgtatcatc ttctttgcca
      541 gagcaatgta cacctcggtc ttcccgctgc cggttatacc ataaatgcaa tgcaccttct
      601 gtggctggtt cataatagcc ctatatattt gttcctgctc ttcagtgagg attacctctt
      661 tagagctatc gccaaaagta aatggctttg tgcgtatgtt atacgatttt cccgaaggta
      721 acgcagtacc cagtgcctca ccaaaataac acacatagtg atttgaaaca aactgtgcta
      781 acctaagtag gcgatcatca aagataggct gtgtatcaag tacctttatg acaggcttca
      841 cgtcaaaatt tggggggtcg tcatggacat gaacaacata accagttgtt ttacggttgt
      901 tgaaattgac aacaacccgc atcccttttg ttatgctagt atcatcagtg ctgtatgtaa
      961 aaaatccatc aacagggaat cctacagcaa catctacaaa catatctatt ttccatccag
     1021 cacagtttta acaagcttca atgtattcca ggctggtttt tttaattcag ggttttttaa
     1081 aagagttata gggtgctcaa tattaaacca ctggataccg ctactgtttt taacaatctt
     1141 ctgtaaggga cgcatttcac ccataacaat aattatctgt ggttttacaa gttctatttc
     1201 ctttgccaga agattctggc agtgtgaaaa catggtaccg ggaagttcac gctggctttc
     1261 acatttaatc atgtgtgtaa tgtagcaggt tttaacatgg acaccaatag ctttcatcat
     1321 cttatcaagc atcgtatcaa catcaggctt caacaccttc ttttcaacag atgaaagcat
     1381 cgatggtggg tttaatatta tcatgattcc tgatgaagca tcaccaccat ggattatatt
     1441 tttttctgta gttttgcatt tattgcattg tgccaccgca tgctgcactt tttcctggaa
     1501 tgtattcaat tcaacaggtg cagcaacatt ttttctgtta aaaaaataac tgatttcatc
     1561 agtataggcc agtaccagtg gtgttcgcct gccatactgt atatgtgtca caattgcttc
     1621 tttaagtaaa tggtacataa tagtgcctgt agttaagttt tgactgatgt aaaatacaca
     1681 ctggcgtgct ataacctgtc aatattaata tgatattgcc ggatctttct gtgcaaattg
     1741 gtgcgctcaa ttcccaacgc tttggcagtg gctgaaatat ttttatcata ctgtttcaat
     1801 gctttgataa tataatcttt ttcaaactgt tcacgtgctt ttttcaaact ggatgtttct
     1861 ttggcaatag tatcctcata atcataggac tcaatatgtt tggtaacatc ctgtacctgt
     1921 ataacttctg atggcaccat aatggtaagc cgttcaatga cattcttaag ttccctgaca
     1981 ttacctggcc acgaataatt aaccagaaat tccatggcac tgtcgttcat ttgtttaaca
     2041 ccaaggccat gctcgcgcgc aaatttttcc agaaagtaat caaccagtaa tggtatatct
     2101 tcacgacgtt ctgaaagagg cggtacataa atggggataa cattgaggcg atagtaaagg
     2161 tcttcacgga agcgtccctg ttcaatggct tcttttacat ccacattggt tgcagcaata
     2221 acacgcacat caactgtaat gacttcattg ccacccacac gctcaaactg ctgttcctgc
     2281 aatacacgca gcaccttggc ctgtgcgctg ggactcatat cacatatctc atcaaggaat
     2341 attgttcctt tgtctgctaa ttcaaatttt cccaagcggc gggcgacagc tcctgtaaac
     2401 gagccttttt catgtccaaa taactcactt tcaataagct catcgggtat tgctgcacag
     2461 ttgactttaa caaaaggctt atcactgcgt ttagattttc gataaatagc tcgtgcaacc
     2521 agttccttgc ctgttccatt ttcaccggtt ataaaaaccc ttgcatttgt tgatgcagct
     2581 gtatcaataa tccgcttaac ctcctgcatt ggaggcgagt tgccaatcat ttcatcttct
     2641 aacgtaatat ttttctttaa tag
//
LOCUS       DAOSZP010000313         2653 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_29944_length_2653_cov_2.791378, whole genome shotgun sequence.
ACCESSION   DAOSZP010000313 DAOSZP010000000
VERSION     DAOSZP010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2653
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29944_length_2653_cov_2.791378"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..326)
                     /locus_tag="PK348_07280"
     CDS             complement(<1..326)
                     /locus_tag="PK348_07280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66053.1"
                     /translation="MKSLSNIIVIGLVLSSATAIVISQQLSHSFRQRIDDYINTYKIS
                     EENIQTQTSTQPILESQAQSPFDYNKGDWTKYIIEPHYKFVVPGAEQLTEEEPYDILS
                     YGSMNL"
     gene            complement(370..1362)
                     /locus_tag="PK348_07285"
     CDS             complement(370..1362)
                     /locus_tag="PK348_07285"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HXK66054.1"
                     /translation="MKVKLKILKSDIERQLKEKYYLKKKQWSKKWDKFLEHGHEKMTI
                     MFIPHNEQKIFNFQISRFTILFFSSLMIIVIVTSAYAIIKNAAVKREEERLLSNYKDI
                     RSQLIRFEQLTNELDKELDDIKPDIEDLYELATNNNNTRSIWESSMISVEDPSMRQYS
                     AILPSEVKTLRVLQKDLICTTNTVKTIKNFIDVRNKVIYDTPSIIPNPGHITSLFGWR
                     RSPFGFGRDFHSGIDIAAPQGTEIRATAPGVVVSSSWGGGYGFMVRIKHKYGYETIYG
                     HCSKIAVFPGQNVQKGQVVAYVGQTGSATGSHCHYEIRLGGVPINPYPYMSKMW"
     gene            1534..1902
                     /locus_tag="PK348_07290"
     CDS             1534..1902
                     /locus_tag="PK348_07290"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HXK66055.1"
                     /translation="MEFGKHMQCKRCGTCCNDVRLAESPEMLKKAYEYWLRMPSVDPK
                     FSEIYLIYPMLTFIYENQSEDLPYHYSCKHFTRDSNGLGVCSIYEIRPRMCRDFPYYE
                     GVELAPEDNVSPYQGCGYNE"
     gene            1985..2530
                     /locus_tag="PK348_07295"
     CDS             1985..2530
                     /locus_tag="PK348_07295"
                     /inference="COORDINATES: protein motif:HMM:NF014012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HXK66056.1"
                     /translation="MIAEDKKNKLLAIVFYSMIAAYSYLVLTIGSGNDILFWSYEEFI
                     FGTVISIILASVTYWLIPIKITGRLLNPFFYLGLIVYFAGPFFIALLIANVEVMYRII
                     SGKIKPAIVKVDPEIQDEMGVYLLVNSITLSPGTLTIGTDPEKRKLFIHCLYWNKSKD
                     YAAVPKDVAGFLHYFLKKLFR"
BASE COUNT          786 a          467 c          520 g          880 t
ORIGIN      
        1 ttgagattca tagacccata ggacagaata tcgtagggct cttcttctgt tagctgttcg
       61 gcacccggta caacaaactt ataatgaggc tcaatgatat attttgtcca gtctccctta
      121 ttataatcaa atggtgattg tgcttgggat tctaaaattg gctgagtaga agtctgggtt
      181 tgaatatttt cttctgaaat tttgtatgta tttatataat catcaatgcg ctggcgaaat
      241 gaatgagata attgctggga gataactata gcagtagctg atgacagcac taaaccaata
      301 acaatgatat tggataatga tttcatgtgt tataattaca aggatgtata gcacagggaa
      361 ataaatatat taccacatct tgctcatata cgggtaagga tttattggca ccccgcccaa
      421 ccgtatttca tagtgacagt ggcttcccgt tgcagatccg gtttgcccta cgtaggcaac
      481 aacctgtcct ttctgtacat tctgcccggg aaatactgca attttggaac agtgcccata
      541 gattgtttca tagccatatt tatgtttaat tctgaccata aatccataac cgccacccca
      601 ggaagatgaa actacaactc ccggagctgt cgctcgaatt tctgtaccct gtggtgctgc
      661 aatgtcaata ccggaatgga agtcccttcc aaagccaaat ggagaacggc gccatccaaa
      721 aagagaagta atgtgcccgg gattgggaat tattgacgga gtgtcataaa taaccttatt
      781 gcgtacatca atgaaattct ttattgtttt tacagtattt gttgtacaaa tcaggtcctt
      841 ttgcaacaca cgcaatgttt tgacttctga gggcagaatt gctgagtact gacgcattga
      901 ggggtcttcc acagaaatca tggatgattc ccatattgac cgggtattat tgttattggt
      961 agccagttca tataagtctt ctatatcagg ttttatgtca tctaattctt tatcaagctc
     1021 atttgtcagc tgctcaaacc gtataagctg tgaccgtata tctttataat ttgacagtag
     1081 gcgttcctct tcacgtttaa cagcagcatt ttttattatg gcatatgctg aagtaacgat
     1141 aactataatc attaaagaac tgaaaaacag aatggtgaaa cgagatatct ggaaattaaa
     1201 tattttctgt tcattatgag gaatgaacat gatggtcatt ttttcatggc catgttcaag
     1261 gaatttatcc cactttttag accattgttt ctttttcaga taatattttt ctttgagctg
     1321 ccgctcaata tcgctcttta gtatttttag ctttactttc atgattcctt tagtaaatag
     1381 cactctttgt atcaggtaat ttagggcaat ggttaaaaat atacaataaa ttgcaataaa
     1441 aaataaaata tgctattatt gtattatatc gtaaaaaaag cctgatttat gcaagttttt
     1501 tttatgcact tcaagcttga agctgcatag ctaatggaat ttggtaaaca tatgcaatgt
     1561 aaacgatgcg gaacatgttg taatgatgta aggcttgccg agtcgcctga aatgctaaag
     1621 aaagcatatg aatactggct gagaatgcct tctgttgacc ctaaattttc agagatatat
     1681 cttatttacc ccatgctgac gtttatttat gaaaaccagt ctgaggattt gccctaccat
     1741 tattcttgca aacatttcac gcgcgatagc aatggcctgg gtgtatgtag tatttacgaa
     1801 ataaggccac ggatgtgccg agatttccca tattatgaag gagttgaact tgctcctgaa
     1861 gacaatgtaa gtccttatca gggttgcggg tataatgaat aagggtaaaa taattccaga
     1921 aaaatgcttg tattattaac aaatgtaata tacggtactc atagttgctt ggtagagttt
     1981 caccatgata gctgaagata aaaaaaataa gctccttgca attgtgtttt attcaatgat
     2041 tgcagcctat tcctaccttg tactgacaat aggttcaggg aatgatatcc ttttctggtc
     2101 atatgaagag tttatttttg gcactgtcat cagtattata ctggcatctg taacgtactg
     2161 gcttataccc ataaagatta caggtaggtt gttaaatcca tttttttatc ttgggttaat
     2221 agtatatttc gcagggcctt ttttcatagc gctgctcata gctaatgttg aagtgatgta
     2281 ccggattatt tccgggaaaa ttaaacctgc cattgtaaaa gttgaccctg aaatacagga
     2341 tgagatgggg gtatatttac tggtaaattc tattaccctg tctcctggca cgctgacgat
     2401 aggcactgat cctgaaaaaa gaaaattatt catccattgt ttgtactgga ataagagcaa
     2461 agattatgcg gctgtcccaa aggatgttgc aggcttttta cactattttt taaaaaagct
     2521 tttcaggtaa agcggatacg cacatgtatg attttaattc tatattcctg ttgtctgcgc
     2581 tgttatgcat ggtgtatgta tgtatcattg taatccgtat tgcattcagc cataatgtcc
     2641 ccgaacgaat agt
//
LOCUS       DAOSZP010000314         2647 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_30029_length_2647_cov_3.936728, whole genome shotgun sequence.
ACCESSION   DAOSZP010000314 DAOSZP010000000
VERSION     DAOSZP010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2647
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30029_length_2647_cov_3.936728"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(29..163)
                     /locus_tag="PK348_07300"
     CDS             complement(29..163)
                     /locus_tag="PK348_07300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66057.1"
                     /translation="MWISLNIIQDMVDIKDIPVADTANRLTMSTAEIDSIEYSHELLI
                     "
     gene            complement(185..1222)
                     /gene="pheS"
                     /locus_tag="PK348_07305"
     CDS             complement(185..1222)
                     /gene="pheS"
                     /locus_tag="PK348_07305"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HXK66058.1"
                     /translation="MYMKKQLQDLYKQTISAIDKATTIDELEQLRVKVLGRKGDLTQI
                     LRSLGTLPQEERVEIGKLSNELKEKIESALENRKTVLTEQQINKIIMEDWLDVTLTMP
                     GTLKHYDRGHLHPISQIQYEIEDIFTSMGFEILDGPEVETDYYNFEALNIPPYHPARD
                     MQDTFWTEDGNLLRTHTSAIQIRGMERLKPPFRVIGPGRVFRYESIDASHENTFYQVE
                     GMMVDKDISVAHLIYFMKTLLKEIFQKETTIRLRPGYFPFVEPGFELDIHCLICGGKG
                     CSVCKYSGWVELLPCGLVHPNVLRYGNIDPDKWSGFAFGLGLNRLVMMRYGINDIRHF
                     LSGDIRFLYQF"
     gene            complement(1430..2329)
                     /locus_tag="PK348_07310"
     CDS             complement(1430..2329)
                     /locus_tag="PK348_07310"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HXK66059.1"
                     /translation="MFFTNQLLFSEQTLNQIHLYAGKWLDFIMVAFTQLGNEMFYILV
                     IPFLFWCVNKRIATIVGIAFLLSAAINDIAKFLFINPRPDAANLAPQIAELNRMYCPV
                     NSPGFPSGHAQSAVVFWGTLAYVVKHRLFTIFSLFLIIAISYSRLYLGVHFLGDVAGG
                     IILGTVIFLCVIALIPHYDRLWNTGIIPAVVFFVILPFVIAIVLTGNEIAKSLGVLSG
                     FAIGFILNNDKSEEITFSIVPALVKFVIGITIILGIKEGLKIVFPSSNVFDFIRYWFM
                     GLWVSYGAPALFMKIPSLSKKSE"
     gene            complement(2353..>2647)
                     /locus_tag="PK348_07315"
     CDS             complement(2353..>2647)
                     /locus_tag="PK348_07315"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HXK66060.1"
                     /translation="FEEAQKGDALALYIFNLIGQYLGIGIANLVNIFNPEAVILGGGL
                     SGAHEFFMPHIIQGVQTLALAGMKEDIHYCVTSYGNYTAAIGAAKYALDNLLE"
BASE COUNT          870 a          523 c          464 g          790 t
ORIGIN      
        1 tggtccagaa aaaccaagca tttcttcttt atataagaag ttcatgggaa tattcaatag
       61 aatcaatctc agcggttgac atggtaagcc ggttggctgt atcagccact ggaatgtctt
      121 taatatcaac catatcctga ataatattta atgaaatcca cataatgcta actccacaca
      181 agacttaaaa ttgatagagg aaacgaatat cgccgcttaa gaaatggcga atatcattaa
      241 tgccatagcg catcatgaca agcctgttta aacccagccc aaatgcaaat cctgaccact
      301 tatcagggtc aatattgcca tacctgagta catttggatg aaccaaccca catggcaaca
      361 gttcaaccca tccggaatat ttacacacac tgcaaccttt gccaccacat ataaggcagt
      421 gaatatcaag ctcaaatcca ggttcaacaa aggggaaata tcctgggcgc aggcgtatgg
      481 tggtttcttt ctggaagatt tctttaagta atgttttcat aaaatagata aggtgcgcta
      541 cagagatatc cttatccacc atcattcctt caacctgata aaatgtattt tcatgtgagg
      601 cgtcaatact ttcatagcgg aaaaccctgc caggaccaat gactctaaat ggcggtttaa
      661 gccgttccat acctcgtatc tgaattgctg atgtatgtgt tctgagcaga ttgccatctt
      721 cagtccagaa tgtatcctgc atatcgcggg cagggtgata cggcggaatg ttgagtgctt
      781 caaagttata gtaatcagtt tctacttcag gaccatcaag aatttcaaaa cccatagagg
      841 taaatatgtc ctcaatttca tactgtattt gcgaaatagg atgcagatga cctctgtcat
      901 aatgttttaa tgtgcctggc atggtaagcg taacatccag ccagtcttcc atgataatct
      961 tgtttatttg ttgttcggtt aaaacggttt tgcgattttc cagagcagat tcaatttttt
     1021 ctttcagttc gttggatagt ttgccaatct caacacgttc ttcctgtggc aatgtgccaa
     1081 gggagcgcaa aatctgagtc aaatcgcctt tgcgtcccag gactttgact cgcaactgtt
     1141 ccagttcatc tatcgttgtg gctttgtcaa ttgcggatat tgtttgttta tagaggtcct
     1201 gaagctgctt tttcatatac atcacctgac taattacaaa tctttattta ctatgaacta
     1261 tcgcccaaaa aaattattat ttgggcgata gttaccggta aataattttt agatttgtat
     1321 tacaaaagaa ccgcttaaaa aaagtcaaca atattactat gaatttttat gtaaattaac
     1381 tatatgttaa ctttactgtt gatgctgtga gatacaatta atcagcgcat tattccgatt
     1441 ttttagaaag ggacggaatt ttcataaata gtgcaggtgc gccatacgat acccataacc
     1501 ccataaacca gtatcggata aagtcaaata cattagatga tggaaacact attttaagac
     1561 cttctttgat accaagaata atggtaatcc caatgacaaa tttaacaagt gcaggtacaa
     1621 ttgaaaaagt aatctcttca gatttatcat tgttgagtat aaagccaatt gcaaaacctg
     1681 atagtacacc caatgatttg gcaatttcat ttcctgttaa aacaattgcg ataacaaaag
     1741 gtagtatgac aaaaaagaca actgcaggaa ttatccctgt attccataac ctgtcatagt
     1801 gtggtatcag ggcaataaca catagaaaaa taacagtgcc taagattata ccaccagcaa
     1861 catcgcctaa aaaatgtaca cctaaataca gtcgtgaata tgaaatcgca ataatcaaaa
     1921 ataatgaaaa tatggtaaat aatcgatgct taacaacata ggccaatgtt ccccagaaca
     1981 ctactgcgct ttgagcatga ccactgggga atccagggct attaacggga caatacatac
     2041 ggtttaattc tgctatttgt ggagcaaggt ttgcagcatc cggccttgga ttgataaata
     2101 aaaattttgc aatatcgtta attgcggcag atagtaaaaa tgcaatacca acaatggttg
     2161 caatgcgttt attgacacac cagaataaaa atggtatcac aagaatataa aacatctcat
     2221 tgcctaattg agtaaatgcc accataataa aatcaagcca tttcccagca tatagatgta
     2281 tctggtttaa cgtttgttca ctgaaaagta actggttggt aaaaaacata atgttcacct
     2341 ctaaaatatg tattattcta aaagattatc cagcgcatat tttgctgcgc ctattgctgc
     2401 tgtgtaatta ccgtatgacg ttacacaata gtgaatatct tccttcatgc ctgctaaggc
     2461 cagcgtttgt acaccttgaa taatgtgtgg cataaaaaat tcatgtgcgc ctgataatcc
     2521 tccaccaagt atcactgcct caggattgaa tatattcact aaatttgcaa taccaattcc
     2581 aaggtattgt ccaattaaat taaaaatata caatgccaga gcatcccctt tttgtgcttc
     2641 ttcaaac
//
LOCUS       DAOSZP010000315         2643 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_30095_length_2643_cov_3.073416, whole genome shotgun sequence.
ACCESSION   DAOSZP010000315 DAOSZP010000000
VERSION     DAOSZP010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2643
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30095_length_2643_cov_3.073416"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..497
                     /locus_tag="PK348_07320"
     CDS             <1..497
                     /locus_tag="PK348_07320"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HXK66061.1"
                     /translation="PIDFGIGINTGDVVLGTVGSDTRMDTTVIGDTVNMSERMEELTK
                     IYKVPIIISENSFNALEKSDEFCIRKIDKVKFLGKTIPITVYEVFDCDDDRIKDMKRN
                     MFNQFNEAIDLYLLGKFNDAYKLLNEYVKIFPHDAVANLYVKRLGLLMDKSVKQWPGY
                     TVVR"
     gene            complement(708..1547)
                     /gene="asd"
                     /locus_tag="PK348_07325"
     CDS             complement(708..1547)
                     /gene="asd"
                     /locus_tag="PK348_07325"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00163.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HXK66062.1"
                     /translation="MNISVKLIVFKILPTRILSRLFGYCTRIPLPAFIMKKIISWYSK
                     KFNVALDEAFIPPGGFKNFDEFFTRNLKRGMRSVQPHPLALVSPVDAMVSACGNINAG
                     QIIQAKGIKYDVASLLPGQFQSQFISGSFITLYLSPGDYHRIHSPVSGTIVGYQYDPG
                     KLYTVQDWLVKMLPGLFTVNERITSYIQSAFGLVAVCKVGAMNVGRISLSYAPVFTNH
                     CFAKPYFYRFDETSRPKVSKGDELGIFHLGSTVIVVTQKPVSFVPNIEGTKVQMGQVL
                     GYF"
     gene            1807..>2643
                     /locus_tag="PK348_07330"
     CDS             1807..>2643
                     /locus_tag="PK348_07330"
                     /inference="COORDINATES: protein motif:HMM:NF017770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjgN family protein"
                     /protein_id="HXK66063.1"
                     /translation="MESIEQTNTDSKIISFHFTGKASEYFKIWIVNGLLTVLTLGIYS
                     AWAKVRTNRYMYGNIYLDNEPFAYTAMPVNILKGRLLALAFFVVYSLSGQYSQTLYMI
                     LTLSLLPIIPWVIVNSIRFRGKYTLYRNIPFIFKGDYLDAFKYFILIYIWVPLTLGII
                     IPYIYYKQKEYIINNLHFGTLPIKYKGKASTFYIAFIASGIISVLTAGFIFGIVSLLG
                     LSHWNQILQKSVENSASIDVSMVVTIIGIYVLFIIAFFIGSAFYKIFILNNVISSIYC
                     GQS"
BASE COUNT          849 a          461 c          464 g          869 t
ORIGIN      
        1 atcctattga ttttgggatt ggcatcaaca caggtgatgt tgtgcttggt acagtagggt
       61 cagatacaag aatggataca acagtcattg gtgatactgt taatatgtcc gaacggatgg
      121 aagagttaac caagatatac aaggtaccaa taataatatc agaaaattca ttcaacgcac
      181 ttgaaaaatc cgacgaattt tgcatacgca aaattgataa agtgaaattt ttaggtaaaa
      241 ctataccaat aactgtttat gaggtttttg attgtgatga tgatagaata aaagacatga
      301 agaggaatat gtttaatcaa tttaatgagg ccattgatct ttatctgctt ggtaaattta
      361 atgatgcgta taaattatta aatgaatatg ttaaaatatt tccacatgat gcagttgcca
      421 atctatatgt aaagaggctt ggtttactta tggataagag cgtaaaacaa tggcctggat
      481 atacagttgt tagatagtta ttgtaatcaa aatgatgtat aattcttcat ttcccggggc
      541 ttggttaggc atttacattt tacaacaaaa tcattgacaa atttttttat attgaccttt
      601 cacacataga ctgcaaaatg cacaaaattc ttgatgtctt gttgacgagc cagttcccgt
      661 ttcaaatttc gtttacccct tacctgttac ctatatacaa aaattaacta aaaatacccc
      721 aatacctgtc ccatctgcac tttggtgccc tctatattgg gaacaaagct aacaggtttt
      781 tgcgtaacta caatgacagt ggaacctaag tggaaaatac caagctcatc accctttgat
      841 acttttggtc gactggtttc atcaaacctg taaaaatatg gctttgcaaa gcagtggtta
      901 gtaaacaccg gtgcatagct taatgaaatc ctgcccacat tcatggctcc aaccttgcat
      961 acagccacca acccaaaggc tgattgtata tatgacgtaa tgcgttcatt tacggtgaaa
     1021 agcccgggca acattttaac cagccagtcc tgtaccgtat acagttttcc gggatcatac
     1081 tggtaaccaa caattgtacc acttacaggt gaatgtatgc ggtgatagtc accgggagaa
     1141 agatataatg tgataaacga tccactgata aactggctct ggaactgccc tggcaacagt
     1201 gatgccacat catacttaat gccttttgcc tgaattattt gcccggcatt aatgttaccg
     1261 catgcactta ccatagcatc aacgggcgat actaatgcaa gcggatgtgg ctgcacagag
     1321 cgcatgcccc gtttcaggtt acgggtgaag aattcatcaa agtttttaaa accaccagga
     1381 gggataaaag cctcatccag cgcaacatta aactttttac tgtaccagct tatgatcttt
     1441 ttcattatga aagcaggcaa gggtatacga gtacaatatc caaacaaccg cgacaaaatt
     1501 cgcgtgggaa gaattttaaa cacaatgagt ttaactgata tattcaaatg agcctcctga
     1561 ttgcagtatg taaaatattt ctattcatca tcgttagtac aagccaaacc atccttcaac
     1621 gaagagtgta aatataaagc atactacttt taaactatgt aagtatttga gaagtatcaa
     1681 atagtatatt gtcaatcagt tttcaggtgt ttatttacta ttctaccatt atttatgtct
     1741 tgacttgttt gaatttatat agtactatac tgcgcattga attttatatt ttaacagggg
     1801 acttttgtgg aatcaattga acaaacaaac actgacagta aaatcatatc attccatttt
     1861 acaggtaaag catccgagta ctttaagata tggattgtta atgggttgct aacagtatta
     1921 actctgggca tttattctgc atgggcaaaa gttcgtacaa acaggtatat gtatggcaat
     1981 atatacctgg ataatgaacc ttttgcatat actgctatgc ctgttaatat tttgaaagga
     2041 agattgttag ctttagcgtt ttttgttgtg tattcattat ccgggcagta ttcgcaaaca
     2101 ttgtatatga tcctcacact atcattattg ccaattatac catgggtaat tgtaaacagt
     2161 atacgattta gaggtaaata taccctttac cgaaatatcc cttttatatt taaaggtgat
     2221 tatctggatg catttaaata ttttattttg atatatatct gggttccgtt aacattagga
     2281 ataattatcc cctatattta ttacaaacag aaggaatata ttatcaacaa tttgcatttt
     2341 ggcacacttc caataaaata taaaggaaaa gcatcaacat tttatattgc tttcattgca
     2401 tctgggatta tatccgtgtt aacagctgga ttcatttttg ggattgtatc actcttgggt
     2461 ttatcgcatt ggaaccaaat cttacaaaaa tctgtagaaa actctgcatc cattgatgtg
     2521 tccatggttg taactatcat aggcatttat gtgttattta tcattgcttt ttttattggt
     2581 tctgcatttt ataaaatctt tattttaaac aatgtaattt ccagtatata ctgtggccaa
     2641 tct
//
LOCUS       DAOSZP010000316         2639 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_30152_length_2639_cov_3.602941, whole genome shotgun sequence.
ACCESSION   DAOSZP010000316 DAOSZP010000000
VERSION     DAOSZP010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2639
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30152_length_2639_cov_3.602941"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="PK348_07335"
     CDS             <1..389
                     /locus_tag="PK348_07335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66064.1"
                     /translation="AIRTKADLCAIKNLPLGTGVEVIASTALEKAYRYSSKPYHFEHV
                     TPYIKENPDTFTIHYFDVDFTNPFPSLRLTVDTQEDYRLASAIYDALYKGSVFTLSDV
                     VAFLEKNPHLVTINASIQQKPMTSHE"
     gene            391..1272
                     /locus_tag="PK348_07340"
     CDS             391..1272
                     /locus_tag="PK348_07340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66065.1"
                     /translation="MDITIITDSGPHIGQGHWQRMLLFAWYCSHNGIPVTLVHKHGIA
                     APDGVSVRLSDSIPQNTSCILRDMRDSSKEEIEYLRAIAPVIVIDDCGEGRQYSTAAI
                     DLLPNIYGNTANTFESTVPLFLYGYNFYQSLSHAPGTIAKDIDCFIYASTFDAALLCE
                     ALGTHYSFVCTDSRTYTTNIALSSSMHYSNLIAQSKVVISHFGISIYEAMLCGCHCII
                     LNPTAYHDKLSHTLQNTNVLVHHELYNHASVPQIMEIFKNFVEQAHTHVDTAHCIRTI
                     QTNLKNFYDALRLIITS"
     gene            1291..>2639
                     /locus_tag="PK348_07345"
     CDS             1291..>2639
                     /locus_tag="PK348_07345"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015907109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HXK66066.1"
                     /translation="MGYSVCYKILQAHLKEGKLEPGTEIGIVIDQTLTQDATGTMAYL
                     QFEAMGIDRVKTEISVSYVDHNTLQMGFENADDHKYLQTMNAQFGIYYSKAGNGICHQ
                     VHLERFAKPGKTLLGSDSHTPTAGGMGMVAIGAGGLDVAVAMGGGLFYLTCPKVINIY
                     LTGKLRPWVSAKDIILKVLQKLTTKGNVGYVIEYTGPGIATLSVPERATITNMGAELG
                     VTTSLFPSDKVTREFLVAQGRENDFVELLPDKDAQYDKVIEIDLSTIEPMIACPHSPD
                     NVVSVESIKGMKVDQVAIGSCTNSSYKDLVTVAKILKGKKIHPKVSCILAPGSKQVLH
                     MLAKEGYLADIIESGVRVMESACGFCIGNGGAPISGGVSLRTNNRNFEGRSGTKDAKV
                     YLVSPETAALSAIEGTLVNPLDMPAQSYPDIKLPKTFIIDDSMIIPPFQSDEPIFRGP
                     "
BASE COUNT          809 a          580 c          552 g          698 t
ORIGIN      
        1 aagcaatccg tacaaaggct gacctgtgtg caataaaaaa cctgccactt ggcaccggtg
       61 tggaagtcat tgcttccacc gctcttgaaa aagcgtatag gtacagcagc aagccctatc
      121 actttgaaca cgtaacacca tacataaaag aaaatccgga taccttcacg attcattatt
      181 ttgatgtaga ttttaccaat ccttttccat cactgcgtct gacagtggac acacaagagg
      241 attatagatt agcatcagca atctatgacg cgctgtataa aggtagcgtt tttacactga
      301 gcgatgtcgt tgcattcctt gaaaaaaatc cgcatctggt tacaataaat gcatccatac
      361 agcagaaacc aatgacatcg catgaataac atggacataa caatcataac cgattcagga
      421 ccacacatag gccagggaca ctggcagcgc atgctactct ttgcatggta ttgctcacac
      481 aatggcatac ccgttacgct tgtgcacaaa cacggcatag cagcccctga tggcgtttca
      541 gtaaggttga gcgatagtat ccctcaaaac accagttgca tactacggga catgcgggat
      601 tcatcaaaag aagaaattga atatttacgg gcgatagctc ctgttatagt catcgatgac
      661 tgcggagaag gccgacagta ttcaaccgct gctattgacc ttttgccaaa tatatacggc
      721 aacactgcca atacctttga atccacagtg ccgctttttc tatacggata taacttttac
      781 cagtcactat cgcacgctcc aggcactatt gcaaaagaca ttgattgttt tatatatgca
      841 tccacatttg atgcagcact tttgtgtgaa gcattgggca cacactatag ctttgtatgc
      901 actgatagta ggacgtacac aacaaacata gcgctatcat ccagtatgca ttattcaaat
      961 ctcattgcac aaagtaaagt agttatatcc cactttggta tatctatata cgaagcaatg
     1021 ttgtgtggtt gccattgtat tattctcaat ccaacagcct atcacgataa actatcgcac
     1081 actttacaaa acacgaacgt gcttgtacac cacgagcttt ataatcacgc ttcagttcca
     1141 caaattatgg aaatatttaa aaattttgtt gaacaggcac acacgcatgt tgatactgcc
     1201 cactgcatac gcacaatcca gacaaatctg aaaaactttt acgatgcgct acgattaatt
     1261 atcacatcat aaacgcaaga ggttgtacca atgggatatt cggtttgtta taaaatattg
     1321 caggcacatc ttaaagaagg aaaacttgag ccaggaaccg aaattggcat tgtcattgac
     1381 cagacattga ctcaggacgc aacgggcacc atggcatact tgcagtttga agcaatggga
     1441 attgaccgtg taaaaactga gatttccgtg tcgtatgttg accacaacac attgcagatg
     1501 ggatttgaaa atgctgatga ccacaaatac ctgcagacca tgaatgctca attcggaatc
     1561 tattattcaa aagcgggcaa tggcatatgt catcaggtgc acttagaacg gtttgcaaag
     1621 ccaggaaaaa cattgcttgg ttccgacagc cacacaccta cagctggtgg catgggcatg
     1681 gtagccattg gtgctggcgg attagatgtt gcagttgcca tgggcggggg gcttttttat
     1741 ttaacctgcc caaaggttat aaatatttat ctaaccggca aactgcgccc atgggtaagc
     1801 gcaaaggata ttatcctgaa agtgcttcaa aaacttacca caaaggggaa tgttggctat
     1861 gtcattgaat atacaggacc tggcattgca acgctttcag tgcctgagcg tgcaaccatc
     1921 accaacatgg gtgccgagtt aggcgtaacc acatcgcttt tccccagtga taaggttacc
     1981 cgtgagtttt tagtagctca gggcagagaa aatgattttg ttgaactgct tcctgataaa
     2041 gatgcacagt acgataaagt tatagaaatt gacctttcaa caattgagcc aatgattgca
     2101 tgcccgcatt cacctgataa tgttgtatct gttgaatcaa ttaaagggat gaaggtggat
     2161 caggttgcca taggcagttg caccaactca tcgtacaaag atctagtcac tgttgcaaaa
     2221 attcttaaag gaaaaaagat acatcccaaa gtcagttgta tattagcacc tggctccaag
     2281 caggtgttgc acatgctggc aaaggaaggg tacctggctg acatcattga aagtggtgtg
     2341 cgtgttatgg aatctgcctg cggtttttgt attggtaatg gcggcgcacc catctctggt
     2401 ggcgtgtcac tgagaaccaa caatcgcaac tttgaagggc gttctggcac taaagatgca
     2461 aaggtttatt tagtaagccc tgaaaccgct gcgctatcgg ccattgaagg aacgcttgta
     2521 aatcctctgg atatgcctgc acaaagctat cctgacataa aattacctaa aacatttata
     2581 atagatgatt caatgatcat tccaccattt cagtcagatg aacctatatt cagaggtcc
//
LOCUS       DAOSZP010000317         2637 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_30173_length_2637_cov_3.428350, whole genome shotgun sequence.
ACCESSION   DAOSZP010000317 DAOSZP010000000
VERSION     DAOSZP010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2637
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30173_length_2637_cov_3.428350"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..818)
                     /locus_tag="PK348_07350"
     CDS             complement(<1..818)
                     /locus_tag="PK348_07350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66067.1"
                     /translation="MLFKQEIISAIIMALFVFMVTISSSKALGRPLTPSMYTVDEGSI
                     EGYLGIDVIQTKSTFSKENMHVQTGILPGSDIRFSASLLQSNVHELSNNTIGDCTISI
                     WHSFGLLCNIVAAGMLIELGLPTAPDVYYNEKTFPTELGFTTFSLHFVAAYNNHSMTL
                     LFSGCYTFIPEPGESVFEGLSFNVTRSSTWRSLFGLNPLRKDSMLYYRRLKDDIASFS
                     LTAVYEMFPVIPYLSFCLGDRINNNPVYGEHGLPVLYKGYNDTLFLVTALGVRY"
     gene            complement(833..1189)
                     /gene="rplT"
                     /locus_tag="PK348_07355"
     CDS             complement(833..1189)
                     /gene="rplT"
                     /locus_tag="PK348_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008595819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HXK66068.1"
                     /translation="MPRATTGKVHHKRREKILKDVKGFYGARKNLWRTAKDARRKKLQ
                     NSYKDRRRKKRDFRALWIMRINAAARLYGISYSRLIDGMKKANILIDRKMLAELAVSD
                     TNAFKAIVEKVTNKAA"
     gene            complement(1208..1405)
                     /gene="rpmI"
                     /locus_tag="PK348_07360"
     CDS             complement(1208..1405)
                     /gene="rpmI"
                     /locus_tag="PK348_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004696338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HXK66069.1"
                     /translation="MPKLKTSSGAKKRFKVTATGKIKRAKGWKSHLLESKSPKRKRAL
                     RKASYVSDSDAKRFARLLPYS"
     gene            complement(1423..1980)
                     /gene="infC"
                     /locus_tag="PK348_07365"
     CDS             complement(1423..1980)
                     /gene="infC"
                     /locus_tag="PK348_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HXK66070.1"
                     /translation="MDRVDTKRFRVNEEIRASMVRLVGEDGGPQVIPLQEALQKAQDK
                     GLDLVEISPNQDPPVVKIIDYSKFKFEQIKKAKEAKKKQKVIQVKEIKLRPSIDLHDF
                     QHKVRHAREFIEEGNKVKFTVMFRGREVVHSDLGFKVLEDVKQALEDVALVETNPSKE
                     GRNITMIMAPMSAAQKKRIQEHNNQ"
     gene            complement(2198..>2637)
                     /locus_tag="PK348_07370"
     CDS             complement(2198..>2637)
                     /locus_tag="PK348_07370"
                     /inference="COORDINATES: protein motif:HMM:NF015113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HXK66071.1"
                     /translation="RFDLYYIGSDGQKHRPYMIHRALLGSIERFIGILTEHYAGRFPV
                     WLSPVQIILVNVGPEQAEYTKDLALKLRLQGIRVKTDLRDETIKYKIRDAIEQRVPYI
                     GVIGGREMQENTIAVRKRGEQKPETMKTDEFVAFIQRQIANKQ"
BASE COUNT          746 a          583 c          427 g          881 t
ORIGIN      
        1 aaataccgaa cacccagagc agtaaccaga aagagtgtat cattataccc tttatacaat
       61 acaggcagtc catgctcccc atatacaggg ttgttattaa tcctatcccc taaacaaaaa
      121 gacagatatg gaattacagg gaacatctca taaactgcag ttaaagaaaa agaggcaatg
      181 tcatccttta atctcctgta ataaagcata ctgtcttttc tcaatgggtt taacccaaaa
      241 agacttctcc aggtgcttga acgtgttaca ttaaatgata gtccttcaaa taccgattca
      301 cctggctctg gtataaatgt ataacagccg gaaaatagca gtgtcattga atggttatta
      361 tatgcagcca caaaatgtaa tgaaaatgtt gtaaatccta actcggtagg gaaggttttt
      421 tcattataat atacatcagg agcggttggt aatcccaatt caatgagcat gccagcagct
      481 acaatattac ataatagccc aaaactgtgc caaatactaa ttgtgcaatc tcctatagta
      541 ttgttcgata gttcatgtac attggactgt aacagtgaag cagaaaacct gatatcagac
      601 cctggcaata tgcctgtttg cacgtgcatg ttttctttac taaatgttga ttttgtttgg
      661 attacatcta tacctaaata cccttcaatt gaaccttcat caacagtgta catggaagga
      721 gttaatggcc tgcctaaagc cttgctgctg gagatagtaa ccataaaaac aaaaagcgcc
      781 atgataatgg cgcttataat ttcttgttta aacagcataa tcaactacat cactatgcag
      841 ctttgttggt taccttttca acaattgcct tgaaagcatt ggtatcgctt actgcaagtt
      901 cagccaacat cttacgatca ataagtatat tggctttctt cataccgtca atgagacggc
      961 tgtatgaaat accgtaaagc cgtgcagctg cattaatacg cataatccat aatgccctga
     1021 aatcgcgttt tttacgcctt ctatccttat acgaattctg caactttttc ctacgggcat
     1081 cttttgctgt tcgccacagg tttttacgtg caccgtaaaa acccttaaca tcttttaata
     1141 tcttttctct tcttttatga tgaaccttac ctgttgttgc tcgtggcata atgtgtacct
     1201 cactgtatta tgaatatggc agtaaccgtg caaagcgctt tgcatctgaa tcagatacat
     1261 atgaagcctt tctcaatgca cgtttgcgct ttgggctttt tgattccaga agatggcttt
     1321 tccatccttt tgcacgtttt atcttgccag tagctgtcac tttaaaacgc ttctttgctc
     1381 cactgctggt ttttaacttg ggcatcgtat acctcgcttt gcttattgat tattatgctc
     1441 ctgaatccgc ttcttctgtg cagctgacat tggagccatt atcatggtta tattcctgcc
     1501 ttcttttgaa ggattagttt ctaccaacgc aacatcttct aaagcctgtt taacatcttc
     1561 cagtaccttg aatcctaaat cgctgtgcac cacttctctt cctctgaaca tgacagtaaa
     1621 ttttacttta ttgccttcct caatgaattc ccgtgcatgg cgcaccttat gttgaaaatc
     1681 atgcaaatca atagaaggtc ttaatttaat ctcttttacc tgaatgacct tctgtttctt
     1741 tttggcttct ttggcttttt tgatttgctc aaatttgaat ttgctgtaat ctattatttt
     1801 tacaactggt ggatcctgat tgggtgatat ttcaaccaga tctaagcctt tatcctgtgc
     1861 tttttgtaat gcttcctgta acggaattac ctgcggacca ccatcttcgc caactaaacg
     1921 aaccatagag gctcgtatct cctcatttac cctgaaacgc ttggtatcaa cccggtctat
     1981 gttaccctcc ttacactttt tctatacaca aaatttataa actaattgat atactatata
     2041 ccgtattata gaaaatattt caacgaaaaa attttccaca tcttatcact gttatatatt
     2101 aatcaacaat aattcttatt aaaatataaa aaatgttact cacgaccctg tacacccttg
     2161 cattactgaa agcagagtaa atgcacatat tacacatcta ttgtttattg gctatctgcc
     2221 gctgaataaa tgcaacaaac tcatctgttt tcattgtttc aggcttttgc tcgccccgtt
     2281 tacggacggc aatagtattt tcctgcattt ccctgccacc aataacacca atatagggga
     2341 ctcgttgttc aatagcatcg cgtatcttat attttattgt ttcatcacgt aaatcagttt
     2401 ttacccgtat tccctgtaaa cgcaatttta gggctaaatc tttggtatac tcagcctgtt
     2461 caggacccac attgaccagt ataatctgta ctggcgacaa ccatacaggg aatcttcctg
     2521 cgtaatgttc ggttaatatg cctataaacc gttcaattga tcctaacagc gccctgtgta
     2581 tcatgtatgg cctgtgcttc tgaccatcgc ttccaatata gtaaaggtca aagcggt
//
LOCUS       DAOSZP010000318         2629 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_30289_length_2629_cov_2.956099, whole genome shotgun sequence.
ACCESSION   DAOSZP010000318 DAOSZP010000000
VERSION     DAOSZP010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2629)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2629)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2629
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30289_length_2629_cov_2.956099"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="PK348_07375"
     CDS             complement(<1..186)
                     /locus_tag="PK348_07375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66072.1"
                     /translation="MSNVKIAYLSILIYTIFCIILSSIVYYFGGVAPGKGFAIGCLLS
                     LILTLLWIAGAIKGMKSN"
     gene            complement(317..1009)
                     /locus_tag="PK348_07380"
     CDS             complement(317..1009)
                     /locus_tag="PK348_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012609351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HXK66073.1"
                     /translation="MNILIFGPNGSGKGTQGAVVQKKFNLPHIESGVIFRENIKKGTE
                     LGVKAKAYIDRGDLVPDDITIPMILNRLQEADCKNGWLLDGFPRNVEQAKALDKALKE
                     AGMALDYVIEIVLDRDSAKKRIMGRRLCEKDNNHPNNIYIDAIKPAEKDGGFVCRVCG
                     GKLSTRADDQDEAAINKRHDIYYDTKTGTLAAVQYFKDISKQNGGKPKIIEVDGTPGV
                     KEVSEALLAKLG"
     gene            complement(1156..1632)
                     /locus_tag="PK348_07385"
     CDS             complement(1156..1632)
                     /locus_tag="PK348_07385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66074.1"
                     /translation="MTFETTTCISYQHCELLEYYTKQYNIPLHTFIYLLINYAAKKEK
                     YRFQAFTRLSYRKRMGKQNWKRVHLYVYHSEYELYLDVKKLWKMSLAKIIAYCLDNVL
                     VEFLNYFNKRLKETNTDNYPNYYESRSYTFHFYKENGIHCCQFHWGPPPEVFQQTA"
     gene            1789..2100
                     /locus_tag="PK348_07390"
     CDS             1789..2100
                     /locus_tag="PK348_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007938024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HXK66075.1"
                     /translation="MIVNIVHVHVKPEHVDDFIKATIENHKNSIKEPGNLRFDVLQCT
                     DKPTTFILYEAYMSPEAAAEHKNTSHYAIWKDTVAPWMAQPRQGTTYTIIAPTEVTLW
                     K"
     gene            2091..>2352
                     /locus_tag="PK348_07395"
     CDS             2091..>2352
                     /locus_tag="PK348_07395"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HXK66076.1"
                     /translation="MEMISFTVGHLPQTYFAKGAIATLPKHTRHFGTHVLLVTGSQSL
                     EKSGRLNDIVAMLDDEGIHIERVAVSGEPSPQLVDDAVKVYAG"
     assembly_gap    2353..2362
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2363..>2629
                     /locus_tag="PK348_07400"
     CDS             <2363..>2629
                     /locus_tag="PK348_07400"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HXK66077.1"
                     /translation="VVVSIGGGSAIDAGKAISAMLPVGGSVTDYLEIIGTKQHSGVKI
                     PFIAVPTTAGTGSEATKNAVLSTTGKNGFKRSLRHDNFVPTIAII"
BASE COUNT          787 a          494 c          486 g          852 t
ORIGIN      
        1 attggatttc atgcctttga ttgctcctgc tatccacagc aatgtaagaa taaggctaag
       61 caggcaccct attgcaaatc cctttcctgg tgccactcct ccaaaataat atacaattga
      121 tgaaagaata atgcaaaaga tagtataaat aagtatgctg aggtatgcta tttttacgtt
      181 actcatagat tgacctatcc tgtattaagc actatataca aatttataat gtataatttt
      241 tagccgtgac aggtcaacta atatttttta ttttaaaaac aggaactgtc acaagacagt
      301 tcctgaatac gatatgttac cctaattttg ccagaagtgc ttcacttact tcttttacac
      361 ccggagtacc gtcaacttca ataattttag gtttaccacc attttgcttt gaaatatcct
      421 tgaagtattg aactgcggct aatgttcctg tttttgtatc ataatatata tcatgacgct
      481 tgttaatagc cgcttcgtcc tggtcatcag cacgtgttga aagcttgcca ccacatacac
      541 gacacacaaa accgccatct ttttctgcag gcttgatagc atcaatgtat atgttatttg
      601 gatggttatt gtctttttcg cacagtcttc tgcccataat gcgttttttt gcactatctc
      661 tatccagaac tatctcaatg acgtaatcaa gggccatgcc tgcttctttt agtgctttat
      721 cgagggcttt tgcctgctct acgtttcgcg ggaacccatc taaaagccag ccatttttgc
      781 agtcagcttc ctgtaaccgg ttcagtatca tagggatagt gatatcatct ggaaccaggt
      841 caccacggtc aatatacgct ttagctttaa caccaagctc ggtgcctttt ttaatgtttt
      901 ctctgaaaat aacacctgat tcaatgtgcg gaagattaaa ttttttttgt acaaccgcac
      961 cctgcgtccc tttaccgctg ccgtttgggc caaatattag tatattcatg gtaccacctc
     1021 ttggattagt atgtgtatag ctgaaacatt cagcatagaa aatcatttca tagcaacagt
     1081 aaaaaactgt atataattgt aaagtaagtt tttatgtagg cgatagctca gtaaacattg
     1141 actagggatg ataagttatg ctgtttgttg gaacacttca gggggtggtc cccaatggaa
     1201 ctggcagcaa tgtataccgt tttctttata aaaatgaaat gtataggaac gactttcgta
     1261 ataattagga tagttatcgg tatttgtttc ttttaatcgt ttattgaaat agtttaaaaa
     1321 ttcaactaga acattgtcca aacaatatgc aatgatttta gcaagagaca ttttccataa
     1381 tttttttaca tctaaatata attcatattc tgaatgatat acatataaat gcacacgttt
     1441 ccagttttgt tttcccatcc tttttctgta tgataaccgt gtaaaggcct gaaaacgata
     1501 cttctctttt ttagctgcgt aatttatcaa aagataaata aacgtgtgaa gtggaatatt
     1561 gtattgtttt gtgtaatatt ccaataattc acaatgttga tacgatatgc aggtggtagt
     1621 ttcaaaagtc atgttctatt cctcccaaag tagtatatat gtaaattata ataccgataa
     1681 ctatcgcata tcacttttcc tgtaaacatt tttttattga aaattatcat tatcgtagtg
     1741 ttcaatttta atagttctat ctatcatatc aaaaaatagg aggttcccat gatagttaat
     1801 atagtacatg ttcatgtaaa gccagagcat gttgatgatt ttattaaagc aactatcgaa
     1861 aatcataaaa attcaattaa agagcctggt aatttacgat ttgatgtgtt acagtgtacc
     1921 gataagccaa caacgtttat tttatatgaa gcatatatgt cacctgaagc ggctgcggag
     1981 cataaaaaca catctcacta tgcaatctgg aaggataccg ttgcaccatg gatggcacaa
     2041 ccacgtcaag gtaccaccta caccatcatt gcaccgaccg aggttacatt atggaaatga
     2101 tttcgtttac tgtggggcat ttgccacaaa catattttgc taaaggagct atcgctacat
     2161 taccaaaaca caccaggcac tttggaacgc atgtattgct tgttactggt agccaatcgt
     2221 tagagaaatc gggaagactc aatgatattg tagcaatgct tgacgatgaa ggaatacaca
     2281 ttgaacgcgt agctgtaagc ggtgagcctt caccacagct tgttgatgat gcggtgaaag
     2341 tatatgcagg cannnnnnnn nngtggtggt gtcaattggt ggtggaagtg caattgatgc
     2401 gggtaaggca atttcagcaa tgctacctgt cggtggaagt gtaactgatt atctggagat
     2461 cattggcaca aaacagcata gtggtgtaaa gattcctttc attgctgtac ctactaccgc
     2521 tggcacgggg agtgaagcaa ccaaaaatgc tgtgctcagt acaactggca aaaacgggtt
     2581 taagcgctct ctgaggcatg acaattttgt gcccactatt gctatcatt
//
LOCUS       DAOSZP010000319         2623 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_30369_length_2623_cov_3.527648, whole genome shotgun sequence.
ACCESSION   DAOSZP010000319 DAOSZP010000000
VERSION     DAOSZP010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2623
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30369_length_2623_cov_3.527648"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(60..>2623)
                     /locus_tag="PK348_07405"
     CDS             complement(60..>2623)
                     /locus_tag="PK348_07405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66078.1"
                     /translation="IKKYDERIAELEEKGTVATKEKKVESEAVENLKKAREEKRKEFE
                     KLKREKLGLTKPVEDKIVKKWSDKLKGLPTEKREAFIRDIVKAIVLKGELKYPDFKKI
                     LAEAIGLGKITKEQGKLIDEYAKAANDVTELGNIAQEDGTPESIKAYEDAVKIAERKA
                     TDLANNLHQDVNITSRLTSLVSLNTLGFGTLLKNIGYNVAQQALIRFPKALIGTAVDY
                     TAYGVSLAANRVYGTPILKPETNVFLAQRGYFSSLGKGIKDALQKIKRGGTQKDYFQS
                     GIQQARIKPIKSWRDLSAWKFGKGDKFLTNREVIDKVLQGTFGVSGEIISRGLFMGDT
                     PARWAAEGARAEQIARLTLGLKSRKDIELFKMFPEQQAKKIFLKRGLSEEKAQAKAED
                     IKRQLQEAGDDATLTMENMLVSAINNIKKYSDAASKESIMGNMANTIGRFAGVLNVPY
                     LKIPVNAAWLAMNIRNPLLALSQSMIFGYDGIKNGNRASLRKSKDWLYIAGVGVAVNT
                     VAAWMANNGIVTPKGTDDDSKKEGAVTNEYDKQFSMNINNLLRGGGVAKDGDVSIDLS
                     WFGSFGAATNTAAQLTKEERARQLSVGDEMLFNLSQGGIDGFTNNVFGGTNALINAYT
                     RGGGYVNQLGLQYFNLMGNVLEPAMISQISKYGMNGQLEIKGNTFGEALSKDIYERSF
                     GVLGKKPEKKLGIFGEPQEKGGSYFGRVARGLTGTEVIDTDKMGYVIYDMYNKTNNAD
                     LIPPSIPKTITVDGKAKTLNYEQQRELTKLIGSSRFELVGAFLEGKAAIMVGNKNQYI
                     DNIKTDEVAKYLKKMYDKGGDLGRARFKALHPEFNSKKDEDTDVDTIEEIEEIGN"
BASE COUNT          623 a          643 c          417 g          940 t
ORIGIN      
        1 ttatggcttt taatagggta aataatcagt atcaacaaaa gtaagtattt tttaagtaat
       61 cagttgccta tttcttcgat ttcttcgatg gtgtccacat cggtatcttc atcctttttg
      121 gaattaaatt cagggtgtag tgccttgaac ctcgcacgtc ccaaatcacc ccctttgtca
      181 tacatctttt tcaggtattt agccacctca tcggttttaa tattatctat gtattggttt
      241 ttgtttccaa ccattatggc tgccttacct tctaaaaatg cacctaccaa ctcaaacctt
      301 gatgaaccga tcaacttagt aagctccctt tgctgttcgt aattaagcgt ttttgccttt
      361 ccgtccaccg tgatggtttt aggtattgac ggaggaatca ggtcagcgtt gttggtcttg
      421 ttgtacatat cgtaaatgac atatcccatt ttatctgtgt caatcacttc cgtgcctgtt
      481 aaccctcttg ctacacgccc aaaataactt cctccttttt cttgcggctc accaaatatg
      541 cctaacttct tttcaggctt tttacctaaa acaccaaatg acctctcata aatatctttc
      601 gataaagcct caccaaatgt attccctttg atctctaact gaccattcat cccatatttt
      661 gatatttgag aaatcatcgc tggctctaat acgttaccca tcagattgaa gtattgtaat
      721 cccaactgat tgacatatcc tcctcctctt gtataggcat tgataagtgc attagtacca
      781 ccgaatacgt tattggtgaa cccatcaata ccaccttgcg acaagttgaa aagcatctca
      841 tcacctaccg atagctgtct tgcacgttct tctttggtaa gctgtgccgc cgtattggtt
      901 gctgcaccga atgaaccaaa ccatgaaagg tcaatgctta catcaccatc ttttgccacg
      961 ccaccgccac gcaaaagatt gtttatattc atgctgaact gcttgtcata ttcattagtt
     1021 accgctcctt ctttctttga atcgtcatca gtgcctttag gtgttacgat accattgttt
     1081 gccatccatg ccgctaccgt attgaccgct acgcctacgc ctgctatata caaccaatcc
     1141 tttgactttc taagtgatgc cctgtttccg ttctttatgc cgtcatagcc aaatatcata
     1201 gactgactta atgcaagtaa tgggtttctg atattcatag ccaaccacgc tgcattaacc
     1261 ggaatcttca agtagggaac attcaagaca cctgcaaatc gtcctatggt atttgccata
     1321 ttacccatga tgctttcctt cgatgcagca tcactatatt tctttatgtt gtttattgcc
     1381 gacacaagca tattttccat tgtcagcgtt gcatcatcgc ccgcttcctg tagctgtcgc
     1441 tttatatctt ctgctttggc ttgtgccttt tcctcgctca accctctttt aaggaatatc
     1501 ttttttgctt gttgttcggg gaacatttta aacaactcaa tatccttcct gcttttcaag
     1561 cctaacgtaa gccttgctat ctgttctgcc cttgcccctt ctgctgccca tcgggcggga
     1621 gtgtcaccca taaataaacc tctcgatatt atttcaccag acacaccaaa tgtaccttgt
     1681 aacaccttgt caataacttc tctattagtc aaaaacttat cgcctttacc aaatttccat
     1741 gctgataaat ccctccatga ttttattggc ttaatacgtg cctgttggat gcctgactga
     1801 aaataatctt tttgtgtacc acctctcttg atcttttgaa gcgcatcttt tattcctttg
     1861 ccaagtgaac taaagtatcc acgttgtgct aagaaaacat ttgtttcagg ctttaagata
     1921 ggagtgccat aaaccctgtt ggcagcaagg gaaacgccat aagcggtata atctaccgct
     1981 gtacctatga gtgctttagg gaatcgtatc aatgcctgtt gcgctacgtt atagcctatg
     2041 ttcttcaaca acgtgccaaa acccaacgta ttgagcgaca caagtgacgt taatcgtgaa
     2101 gtgatgttca cgtcctgatg caggttatta gctaagtcag tagcctttct ttcagctatc
     2161 ttaaccgcat cttcgtatgc ttttatgctt tcgggcgttc catcttcctg tgctatatta
     2221 ccaagttctg ttacatcatt tgctgctttt gcgtattcat caatgagttt accttgttct
     2281 tttgttatct tgcctaatcc aatagcttct gcaagtatct tcttgaaatc tggatatttc
     2341 agttcacctt ttaacacaat agcctttaca atatccctga taaatgcctc acgcttttct
     2401 gttggtagtc ctttcagttt gtcgctccac ttctttacta tcttatcttc aacgggtttg
     2461 gtaagtccta atttttcacg ctttaatttc tcaaactctt tgcgtttttc ttcacgtgct
     2521 ttttttaagt tttcaactgc ttctgattcc accttttttt ctttggttgc taccgttcct
     2581 ttttcttcaa gttcagcaat tctttcatca tactttttga ttg
//
LOCUS       DAOSZP010000320         2622 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_30384_length_2622_cov_3.560187, whole genome shotgun sequence.
ACCESSION   DAOSZP010000320 DAOSZP010000000
VERSION     DAOSZP010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2622
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30384_length_2622_cov_3.560187"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..357)
                     /locus_tag="PK348_07410"
     CDS             complement(<1..357)
                     /locus_tag="PK348_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017312618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HXK66079.1"
                     /translation="MQNKIIYLDNNATTMVAPEVVEAMMPFFMERYGNPSSMHDFGGD
                     VAHDVENARHQVALMLGADHDYEIVFTSGATESDNMAILGTLQYYRDKKHIVTTKVEH
                     PAVLNLCRQLEREGYSV"
     gene            complement(357..1196)
                     /gene="nifU"
                     /locus_tag="PK348_07415"
     CDS             complement(357..1196)
                     /gene="nifU"
                     /locus_tag="PK348_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015719728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein NifU"
                     /protein_id="HXK66080.1"
                     /translation="MWEYTEKVKELYKNPKNVGEIPDADVVVEVGSIVCGDALTLYLK
                     LDGNKIIDAKFKTFGCGSAIASSSALTEMIKGKTIEEAAKITNKDIVEFLGGLPDQKM
                     HCSVMGQEALEKAIKKIKGHAVEDEYEDEGAIVCQCFGISENLIRRVINENNLTTVEE
                     VTNYTKAGGACGSCIPKIEDIIKEELAKGAAQKIASVQKPMSNLKRMQLVEETIQNVI
                     RPVLMRDGGDIELIEIDGKKVYVALRGHCAHCVISDVTMKNLVQEKLREFVESDIEVI
                     EVK"
     gene            complement(1362..2525)
                     /locus_tag="PK348_07420"
     CDS             complement(1362..2525)
                     /locus_tag="PK348_07420"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HXK66081.1"
                     /translation="MRKVILKQGKEKSVLQHHPWVFSGAIAHAEVSDGQLAAVCAHDG
                     TVLAWGYYNSRTDIAVRLLSFGDRKPDTDLFVNRICASLTLRKVTGITQCTNAYRLIH
                     SEGDMLPGLIVDWYKGHCVMQSLTLGMDKLKVTIAQIINDVLKPESIYERSDHEGRKK
                     EGLHEQSTQVYGYTPEIIEVNENGMLFLVDVRRGQKTGFFCDQRDNRKKVKEIANGRD
                     VLNLFSYTGGFSVAALLGGAKSAVSVDGSGSALQVAHRNCEMNNVGQAHEIIKADVFD
                     YINNNDITQNLIICDPPALAKNKASVNNAARGYKELNLKIISKCPADSFLLTCSCSRF
                     IDHKLFQQIVFAACNDAGRDACIVGRYSQPADHPVNIYCPETEYLKALLLYIY"
BASE COUNT          771 a          625 c          399 g          827 t
ORIGIN      
        1 tactgaatat ccttcccgtt caagctggcg gcacaaattg agtactgctg gatgttcaac
       61 ttttgtagta acaatatgtt ttttgtcacg gtaatactgc aatgtaccca gtatagccat
      121 attatcgctt tcggttgctc cactggtaaa tacaatttca taatcatgat cagcacccaa
      181 catcagtgca acctgatggc gggcattctc aacatcatgg gcaacgtcac caccaaaatc
      241 atgcatgctg gaagggttgc cgtatcgttc cataaaaaaa ggcatcattg cttctacaac
      301 ttcgggagca accattgttg ttgcgttatt atcaagatat ataattttat tttgcattac
      361 tttacctcaa ttacttcaat atcgctttca acaaactcac gtaacttctc ctgaaccaga
      421 ttcttcatgg ttacatcaga aataacgcag tgagcacaat ggccacggag cgctacatac
      481 actttctttc catcgatttc aataagttca atatcaccac catcacgcat taaaaccggg
      541 cgaatgacat tttgaatggt ttcttcaaca agctgcatcc tctttaaatt ggacattggt
      601 ttttgaactg aagcaatctt ttgcgcagca ccttttgcca attcctcttt aataatgtcc
      661 tcaatttttg gaatacacga tccgcaagca ccacctgcct tggtatagtt agtaacctct
      721 tccacagtag taagattgtt ttcattaata acccgtcgta tcaaattttc agaaatccca
      781 aaacactggc acacaatagc accttcatcc tcatactcat cttcaactgc atgccctttg
      841 attttcttta tagctttttc aagcgcctcc tgccccatta ccgagcagtg cattttttga
      901 tcgggcaatc cacctaaaaa ctcaacaata tctttattgg taatctttgc agcctcttca
      961 attgttttac ctttaatcat ttctgttaaa gctgatgacg aagcaatagc acttccacaa
     1021 ccaaaggttt taaacttggc atcaataatt ttattgccgt caagcttaag ataaagtgtc
     1081 agagcatcac cacatacaat actaccaacc tcaactacca catctgcgtc aggaatctca
     1141 cccacatttt ttggattttt atataattct tttacttttt cagtatattc ccacataaaa
     1201 ctctccctta tgatatgtac tattgatgaa taaagccttc ataccagata aaaatcaata
     1261 tatttttata ccaataatac atatcatatt atatactacc attatagtag tatttaacat
     1321 ttacgtcaat tattttttgt attttttatt ccctgaaatt ctcaataaat atacaaaagc
     1381 agcgccttta aatattcagt ttcggggcaa tatatattca ccgggtgatc ggcgggttgt
     1441 gagtatcgcc ctacaatgca tgcatccctt ccggcatcat tgcaggcagc aaacacaatc
     1501 tgctgaaaaa gcttatggtc aataaaccgc gaacatgaac aggtaagcaa aaacgaatca
     1561 gccgggcact ttgaaataat ttttaaattt aattctttat atccccgcgc ggcattattt
     1621 accgatgctt tatttttagc caatgccggg ggatcgcaaa taatcagatt ctgcgtaata
     1681 tcgttgttat taatataatc aaatacgtca gctttaataa tttcatgtgc ctgccccacg
     1741 ttattcatct cgcagttacg atgtgcaacc tgtaacgcag agccagaacc atcaacgcta
     1801 actgcgcttt tagcaccacc caaaagagca gctaccgaaa atccaccggt atacgaaaaa
     1861 agattcagca catcccgccc atttgcaatt tccttaactt ttttcctgtt atcacgctga
     1921 tcgcaaaaga atcctgtttt ttgtccccgt cgtacatcaa ccaaaaatag catgccattt
     1981 tcatttacct caattatttc cggtgtgtac ccgtacacct gcgtactttg ttcatgtaac
     2041 ccttcttttt ttcttccttc gtggtcactg cgttcataaa tgctttccgg ttttagtaca
     2101 tcattaataa tttgagcgat agttactttt aatttatcca tgcctaatgt taaactctgc
     2161 atgacgcaat gccccttata ccaatcaaca attaatcccg gcagcatatc gccttctgaa
     2221 tgtatgaggc gatatgcatt ggtgcattgc gtaattcccg ttaccttacg taatgtaagc
     2281 gaagcacata tccgattgac aaaaaggtca gtatccggct tgcgatcacc aaatgaaagt
     2341 aaccgtacag caatatcagt gcggctgttg tagtatcccc acgcaagcac ggtgccatca
     2401 tgtgcacaca ctgctgctaa ctgtccatct gacacttctg catgagctat cgcccctgaa
     2461 aaaacccatg gatgatgctg caatactgat ttttctttac cctgttttaa gattacctta
     2521 cgcatattta ttgtgccagt tttgattgat ataccatcaa agaatgatac acaacaatac
     2581 catatgcaac agccacattc agtgaatgtt taatcccaaa ca
//
LOCUS       DAOSZP010000321         2610 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_30561_length_2610_cov_3.241487, whole genome shotgun sequence.
ACCESSION   DAOSZP010000321 DAOSZP010000000
VERSION     DAOSZP010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2610
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30561_length_2610_cov_3.241487"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1128)
                     /locus_tag="PK348_07425"
     CDS             complement(<1..1128)
                     /locus_tag="PK348_07425"
                     /inference="COORDINATES: protein motif:HMM:TIGR01977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HXK66082.1"
                     /translation="MIYLDNAATSYPKPQSVIKAVADCLQDYCANPGRSGHKLAIEAA
                     RKVFEARETVAQFFNVTDSRNVIFTSNATMAINCACAAILEDSDHVVVSSMEHNAVMR
                     PLRYLEKEGKISLSFIESGSNGKITGDVTQLLQPNTRLVVINHASNVNGVVQDITSIT
                     AKIKAYSPSVTVLIDASQSAGVLPIDMQKIGCDFLAFTGHKALYGPQGIGGLIINTDR
                     PLKPFIMGGTGSKSESQYQPEFLPDKYESGTVNLPGIVGLKAGIEYVWPQMEKIRTEE
                     MKHIKKVIECLSGIKGITVYWSNSFEHQIGVVSFSHESISCSEIGQRLNDEYSICVRI
                     GLHCAPLAHETIGSYPDGTVRISVGTFTEDRDIDTFCRAIEQ"
     gene            complement(1164..>2610)
                     /locus_tag="PK348_07430"
     CDS             complement(1164..>2610)
                     /locus_tag="PK348_07430"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HXK66083.1"
                     /translation="SVPRGADLIKVAQNINKPIVVYKSNTTQAGKRAAMSHTAAMSND
                     EDIIDAAFEKAGIIRIHNYHDFFAVAKAFKIPPMRGKRIMVMSPAGGFAVMTADLCEQ
                     AGFEFADPGEEFYQGLQKFSNAGVIHFSNPLDLGDIYDPAFTAHVVYSVMHNPNVDGA
                     VYVSQRPQMPDRESVFSRMFLADLSKETWGAIVSSAKPLGICLLGLSRVMAQIKRTVN
                     FPIFNSPEEMVRALAYQMKFNTRQLRQKQDESCTVSFDGALQWIKRNTGEIGEDAMEL
                     LTTIDVPVPPSGIATTETEAVAIATRIGYPVVCKLVSKEILHKSDVGGVIVNVTSDDA
                     VKQAFATIHANVLSHKHDATIEGVRISKMAANGVDMFVGSKYDDSFGQVILYGFGGIY
                     VEVFKDVACSLCPAPKDEIKEKISKLKSYALLKGARGQEPKDIDAYVDIIWKLSHLLA
                     RAPQIKEMDLNPVRVFDTGCQVLDIRIKIVQ"
BASE COUNT          745 a          612 c          458 g          795 t
ORIGIN      
        1 ttgttcgata gccctgcaaa atgtatcgat atccctatcc tctgtaaatg tgccaacaga
       61 tatccttacg gtgccatctg gataactgcc tatagtttca tgcgcaagag gtgcacagtg
      121 caatccaata cgaacacaaa tggaatattc atcattaaga cgctgaccta tttcactgca
      181 cgaaattgat tcatgggaaa aagatacaac acctatttga tgttcaaaag aattactcca
      241 atatacagtt atacctttaa ttccagacaa acactctatt acttttttga tatgtttcat
      301 ttcttcagta cgtatctttt ccatttgtgg ccaaacatat tctattccag ccttcaatcc
      361 cacaataccg ggaaggttta ctgtaccact ttcatattta tcaggcaaaa attcaggctg
      421 atactggctt tcggatttgc ttcctgtgcc tcccataata aaaggtttta atggcctatc
      481 tgtattaata attaagccgc caatcccctg tggcccatac aacgctttgt gccccgtgaa
      541 cgctaaaaaa tcacatccaa tcttctgcat atcaattggc aatactccgg cactctgcga
      601 tgcatcaata agcaccgtaa ctgaaggact atatgcttta atctttgcag tgatggaagt
      661 aatatcctgt actactccat tgacattgga tgcatgattt atgactacaa gcctggtgtt
      721 gggttgtaat aattgtgtaa catcacctgt aatttttccg ttgctaccag attcaataaa
      781 agataatgat atttttcctt ctttttccag ataccgcaat ggtcgcatca ccgcattatg
      841 ttccatagat gaaactacta catggtcact atcttccaga attgcagcac acgcacaatt
      901 tatggccatg gttgcatttg aagtgaaaat aacgttacgg gaatctgtaa cattaaaaaa
      961 ctgtgcaact gtctcccgcg cttcaaacac ctttcgtgca gcctctattg caagtttgtg
     1021 ccctgaacgc ccgggatttg cacaataatc ctgcaaacag tcagctacag cttttattac
     1081 agactgaggt ttagggtaac tggtagcggc attatctaaa taaatcatac gtaaagcgta
     1141 gcaccataca atggttattt caattattgc acaatcttta tcctgatatc aagcacctga
     1201 cagccagtat caaatacacg tactggattt aagtccattt cttttatttg tggcgccctt
     1261 gccagaagat gtgaaagttt ccagattata tccacatacg catcaatgtc ctttggttcc
     1321 tggccacgag caccttttaa taatgcatac gatttaagct ttgaaatctt ttcttttatt
     1381 tcatcttttg gtgctggaca caacgagcag gcaacatcct taaatacttc aacatagatt
     1441 ccgccaaagc catacagtat cacctggcca aacgaatcat catatttact gccaacaaac
     1501 atatctacac catttgcagc catttttgaa atgcgcactc cttcaattgt tgcatcatgt
     1561 ttgtgcgata gtacatttgc atgaatcgtt gcaaaagcct gcttcaccgc atcatcactg
     1621 gttacattaa caatcacacc gccaacatcc gatttgtgca atatctcttt tgatacaagt
     1681 ttacatacca ccggatagcc aatacgagtg gctatcgcca ctgcttctgt ttccgttgtg
     1741 gcaatacccg atggtggcac cggtacatca attgttgtaa gcagttccat tgcatcttca
     1801 ccaatttcac cggtattgcg tttaatccac tgcaatgcac catcaaacga cacagtgcat
     1861 gattcatcct gcttttgcct gagttgtcgt gtattaaatt tcatctgata tgcaagtgcg
     1921 cgcaccattt cttcaggtga attaaatata gggaaattta ctgtacgctt tatctgtgcc
     1981 atcacgcgag ataaaccaag taaacaaata cccagaggct tagctgatga tacaatggca
     2041 ccccaggttt cctttgaaag gtcagctaaa aacattcgtg aaaaaacact ttctctatca
     2101 ggcatctgtg gacgctggct tacatacacc gcgccatcca catttggatt gtgcattact
     2161 gaatacacaa catgggcagt aaatgccggg tcatagatgt cacctaaatc cagtggattt
     2221 gaaaagtgga ttacacctgc atttgaaaat ttttgaaggc cttgataaaa ttcttcaccg
     2281 ggatctgcaa attcaaaacc tgcctgttca cacaggtcag cggtcataac tgcaaaaccg
     2341 ccggcagggc tcataaccat gatgcgctta ccccgcatag gtggaatctt gaaagctttt
     2401 gccacagcaa aaaagtcatg gtaattatgt attcggataa tccctgcctt ttcaaatgct
     2461 gcatctatga tatcctcatc attgctcatg gcagccgtgt ggctcatggc agctcttttt
     2521 cctgcctgtg tggtattgga tttatacaca acaataggct tattaatatt ttgggctact
     2581 tttattaagt cagctccccg tggcacactt
//
LOCUS       DAOSZP010000322         2610 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_30563_length_2610_cov_3.055969, whole genome shotgun sequence.
ACCESSION   DAOSZP010000322 DAOSZP010000000
VERSION     DAOSZP010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2610
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30563_length_2610_cov_3.055969"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /gene="ssrA"
                     /locus_tag="PK348_07435"
     tmRNA           <1..268
                     /gene="ssrA"
                     /locus_tag="PK348_07435"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(271..921)
                     /locus_tag="PK348_07440"
     CDS             complement(271..921)
                     /locus_tag="PK348_07440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66084.1"
                     /translation="MMVYSHYNRLYNIFIILITLSIYITCSSGRPKELHEDLPKLKTI
                     KKVAVMVRIPSSSPIEYARYTKTMQAMIAGYKHTKEMIVISEDIPALTVFQSNDDRFF
                     QTSLDGEFLYFKATGIVNSYCFKNKTELQSLFEKYGCDLLVVYEPYGVVSYGMGFIDY
                     DSVMVILNKDLKITYFDYNHDRKETNEFSTEVLWDFLLNEINTRCVNTLIDLGFIK"
     gene            complement(1075..1650)
                     /locus_tag="PK348_07445"
     CDS             complement(1075..1650)
                     /locus_tag="PK348_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HXK66085.1"
                     /translation="MKNVIFAGVGGQGVILASKILMQVAMNAGYDVKESEVHGMAQRG
                     GSVDCHVRFDKKVYSPLIEKGTADFVVALELLEAMRKLDYLSPQGMLIVNRFKVNPAP
                     VEAGLAKYPDDIESWLCSNVPRCHVVDTTQALKEAGSNRALNIVMLGVLSNFLEFDQK
                     AWEEAIRQSVKEKFVDMNLKAFNLGKSITVS"
     gene            complement(1664..>2610)
                     /locus_tag="PK348_07450"
     CDS             complement(1664..>2610)
                     /locus_tag="PK348_07450"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HXK66086.1"
                     /translation="IEEQMLKLKEYSNTCELNTIEWNSKKRGIITNGVAYQYVKDVCP
                     DASILKIGVAWPLPDAKIHEFCSNVEELYVVEELDPFIEDYVRALGYNPIGKEIIPIC
                     GELTPEIIDMAINKKRPKNEIPVYSGSIPGRPPALCKGCPHGFVFEVLKKLDVVVNGD
                     IGCYTLAVLPPYSAMHTQGCMGASIGMHLGFEKARGDEMARNSVAVIGDSTFVHSGIT
                     PLIDLVYNKGTGTVIILDNRVTAMTGHQDHPATGRTLKGEETHELDLELLCKAVGVKR
                     VRKIDPKNIEEFEKAVREEIAAKEPSVIISLRKCILTK"
BASE COUNT          771 a          588 c          414 g          837 t
ORIGIN      
        1 aataagtgca aataacgatt tagctctcgc agcttaatcc ctgcgggcgt ctttatcgat
       61 cccgctcctc tggggatgat aaaggcgtga gatacagagg atagttattg agttttgatc
      121 ctggctcaat agcgaaattt taaggatttg ctcactgagg cgggttcctg ttcccctaac
      181 cagtgataag ataaacgaac aggataagca tgtagaggct gtaggtgtgt gttgcaggac
      241 gcgggttcga ctcccgccgt ctccaaaaag tcatttaata aagccaagat caattaatgt
      301 atttacacac cgtgtattaa tttcattcag cagaaaatcc cacaatactt cagtggaaaa
      361 ttcatttgtt tcttttcggt catgattgta atcaaaatat gtaattttca aatctttatt
      421 taaaataacc atgactgaat cataatctat gaatcccatt ccatacgata ctactccata
      481 tggctcatag actaccagta aatcacatcc atacttttca aataacgatt gcaattcagt
      541 tttattctta aagcaatatg agttcacaat ccctgttgcc ttgaaatata aaaactcacc
      601 atccagagat gtttgaaaaa atctgtcatc atttgactga aaaacagtaa gtgctggtat
      661 gtcctctgaa ataacaatca tttctttggt atgtttataa ccagcaatca tcgcctgcat
      721 tgtttttgta tatcgtgcat actcaatggg gctgcttgag ggaattctaa ccatcacagc
      781 tacttttttg atggttttca acttgggtag atcttcatga agttctttag gtcttccaga
      841 actacatgta atatatatag acaaagtaat gagaataata aaaatgttat aaagccgatt
      901 gtaatgtgaa tataccatca atggccttac ctcccttaca ataggattta ctaactcacc
      961 taaagcctta gcttcaatat cagagcagcc tctaaggggc taaagaagca tattacctct
     1021 tgatcattat gatataggaa gccccaaaat atcttttctt taaatttata aaaatcagga
     1081 cacagtaata cttttcccta aattaaaagc tttcaggttc atatctacaa atttttcttt
     1141 aacactctgt cgtattgctt cttcccatgc cttctggtca aattcaagaa aattggataa
     1201 aaccccaagc attacaatat tgagcgccct gtttgagcca gcttctttta atgcttgcgt
     1261 tgtatcaacc acatggcatc gggggacatt gctgcatagc cagctttcaa tatcatcagg
     1321 atattttgca agccctgctt ccacaggggc agggtttact ttaaacctgt taacaataag
     1381 catcccctgt ggtgacagat agtccagttt tctcattgct tcgagcaact caagtgccac
     1441 cacaaaatcg gctgttcctt tttcaataag tggtgaatat acctttttat caaatctgac
     1501 atggcaatca acactacctc cacgctgtgc catgccgtga acctcagact cttttacatc
     1561 ataccctgca ttcatagcaa cctgcattaa gattttactt gcaagtatta ccccctgtcc
     1621 cccaactcct gcaaaaatta cattcttcat acttttctac cctctacttt gtcaaaatac
     1681 atttacgcaa cgaaataatc actgatggct ctttagctgc tatctcttca cgaacagctt
     1741 tttcaaattc ttctatattt tttggatcaa tcttccgtac tcgtttcaca ccaactgcct
     1801 tacataaaag ctctaaatcc agttcatgcg tttcttcgcc ttttaacgtc cttcctgtag
     1861 ctggatggtc ttgatggcct gtcattgctg ttacacggtt atccagtatt atgaccgtac
     1921 ctgtgccttt attatagact aaatcaatta aaggtgtaat tcctgaatga acaaatgttg
     1981 aatcgccaat gactgcaaca ctgtttcgcg ccatttcatc accacgcgct ttttcaaaac
     2041 ctaaatgcat accaatgctt gcacccatac aaccctgagt gtgcattgca gaatatggtg
     2101 gcaacactgc cagggtatag cagccaatat caccattaac aacaacatct aattttttta
     2161 atacctcaaa tacaaagcca tgagggcacc ctttacacag cgccggcggt cttcctggta
     2221 ttgaaccaga atataccggt atttcatttt ttggcctctt cttattaatt gccatatcaa
     2281 ttatttcagg agttaactct ccacatatag gaattatttc tttacctatt gggttgtatc
     2341 ctaatgcacg gacataatct tcaataaatg ggtcaagctc ttctaccaca tacagctctt
     2401 caacgttgct acaaaattca tgaatcttag catcaggaag aggccatgct acaccaattt
     2461 ttaatataga cgcatccggg cacacatcct taacatactg gtatgcaaca ccattggtaa
     2521 taattccgcg ctttttagaa ttccattcta tagtattcag ttcacaggta tttgagtatt
     2581 ctttcaactt cagcatttgt tcttcaataa
//
LOCUS       DAOSZP010000323         2608 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_30586_length_2608_cov_6.117509, whole genome shotgun sequence.
ACCESSION   DAOSZP010000323 DAOSZP010000000
VERSION     DAOSZP010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2608
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30586_length_2608_cov_6.117509"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2102
                     /locus_tag="PK348_07455"
     CDS             <1..2102
                     /locus_tag="PK348_07455"
                     /inference="COORDINATES: protein motif:HMM:NF019924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HXK66087.1"
                     /translation="PITKHYSYYTGKELKASKLYTVVSYTERIKLLKLEPVDDITPLY
                     DEFLRDSLFRLYSISNPVYFKSNFMFCETIAHTASFDEFIHFLQKHIISLFYPQYKNF
                     IEIVFKRILFYMFKFGFTHGNDVLKLIDNLSSHHDTTIAELGKSIQFHNRRGPIVFIS
                     AEAEPFSKSGGLANVVYELPRELAKLGEEVIVITPKYRHGDENAIKKMAYALQQYKAV
                     YTGKNVRFMIENNTYEAGVHYAQVDGIHYYLFDHHEFFDGLYWGYTGEEKLRRRIAFA
                     RASAELITTFGLSPLFVLTNDAYTGIFNGIVRSDHIYYANPNFKRTSFFHIIHNGGWQ
                     YFDSYHRHENGKDLFSLFNLPHWRYNDFSDPTEYDKINCMASGIRFSDRVITVSPSYA
                     KQIEKACDGLEKILHNVIGISNALGMDFKNRITRQFYHSGFIDEYYPEMIEGLKNNPE
                     VYQSVRTRYPQILEGVTACEHITDSLQREYITHIRNKLCWQCKLGFAINPDITLFSMI
                     HRIAEQKGFQLLLDASYGIFTTLRYQGIIGGAVAWGDHRGSELATGLINLQNYYQKSV
                     SVTLGFQDISIPLMCSDVFLMPSLHEPGGISQLEALACGCLVVARATGGLRDTVIPIR
                     VREQSVEGNGFLFSDFNAASFYDAMQRCAAFFKNSNDTVKALARKNAMESVYYWDKPA
                     KEYIDVLYSHKEIIRVV"
     gene            2209..>2608
                     /locus_tag="PK348_07460"
     CDS             2209..>2608
                     /locus_tag="PK348_07460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66088.1"
                     /translation="MKTKLYTILYTIIPLLCIVVLLSCTTNEKSAVSTIVHSINGQPV
                     IPFTANKLYIEPFKNETGTQHYEDSIKKRIVEKIVANGRLAIVTSEEASDVKLMGTLQ
                     SLQIQPITIDYAGIVKKKRLLIIAVISLVNT"
BASE COUNT          833 a          498 c          492 g          785 t
ORIGIN      
        1 cgcctatcac aaaacattac agttactata ccggaaaaga actcaaagca tcgaaactct
       61 acacagtggt gtcctacact gaaagaatta agcttttaaa acttgagcct gttgatgata
      121 taactcctct ctatgatgaa ttcctgcgtg actcattatt tagattgtat tcaataagca
      181 atccagttta ttttaaatca aatttcatgt tttgcgaaac tatcgcgcat actgcttctt
      241 ttgatgaatt tatacacttt ttacaaaagc atattatctc attattctac cctcaatata
      301 aaaactttat tgagattgta tttaaacgca tactatttta tatgtttaaa tttggtttta
      361 cccatggcaa tgatgtttta aaattaattg acaacctttc atcacatcat gataccacta
      421 ttgctgaatt gggaaaaagt atacaatttc acaacaggcg tggacccatt gtatttatct
      481 cagcagaagc agaaccattt tcaaaaagcg gtgggcttgc caacgttgtt tatgaactcc
      541 cccgtgagct tgcaaagtta ggcgaggagg taatcgtcat aaccccaaaa tatcgtcatg
      601 gggatgaaaa tgccataaaa aaaatggctt atgcattaca gcaatacaaa gcagtgtata
      661 caggaaaaaa tgtacgcttc atgattgaaa acaacaccta tgaagctggc gtccactatg
      721 cacaggttga tggcatccat tactatttat ttgatcacca tgaatttttt gacgggcttt
      781 actggggcta tacgggcgag gaaaaattgc ggcgccgtat tgcctttgca cgcgccagcg
      841 ctgagcttat aaccaccttt ggattgagcc ctctttttgt attaaccaac gatgcttaca
      901 ccggaatatt caacggcatt gtgcgaagtg accatatata ttatgctaat ccaaatttta
      961 aacgaacatc ttttttccat attattcaca atgggggatg gcaatatttt gattcatacc
     1021 acagacacga aaatggcaaa gatctttttt cactgtttaa ccttccccac tggcgataca
     1081 atgatttttc tgatccaacc gaatatgata agattaactg tatggcatcg ggaatacgtt
     1141 tttctgaccg tgtcatcacg gtaagcccct catacgcaaa gcagattgaa aaggcatgtg
     1201 atggtcttga aaagatacta cacaacgtta ttggtataag taacgctctg ggaatggatt
     1261 ttaaaaatcg tattaccagg cagttttacc attcaggatt tattgatgaa tattatcctg
     1321 aaatgatcga aggacttaaa aacaatccag aagtttatca atcagtgcgt acacgttacc
     1381 ctcagatact tgaaggagtc acagcatgtg aacacattac tgactcattg caacgagaat
     1441 atataacgca tatccgtaat aaactatgct ggcaatgtaa actgggattt gctatcaatc
     1501 ctgatatcac attattttct atgatccacc gcattgccga acaaaaaggc tttcaacttc
     1561 ttctggatgc atcatacgga atatttacta cattacgcta tcaagggatt attggtggcg
     1621 cagtcgcatg gggcgaccat cgaggaagcg agctggcaac tggactcatt aatctacaaa
     1681 attactatca aaaaagtgtc tcggttaccc ttggttttca ggatatcagt attccactta
     1741 tgtgctctga tgtatttctc atgccttcat tgcacgagcc aggcggcatt tctcaattag
     1801 aggctctggc atgtggttgt cttgtggttg ctcgggctac aggaggattg cgtgacaccg
     1861 taatacccat acgtgtcaga gaacaatcag ttgagggtaa tggttttctt ttttcagatt
     1921 ttaatgctgc atcgttctac gatgccatgc aacgatgtgc tgcatttttt aaaaacagca
     1981 atgataccgt aaaagcactt gcgcggaaaa atgcaatgga gtcagtctac tattgggata
     2041 agccggctaa agaatatatt gatgtacttt attcacacaa agagattata cgggtggtat
     2101 aaaataaaag agccttccac agacactatg agtacgagtg ggaggccctg gagtgtaaaa
     2161 tgatcttatt ttgttatcgg gaatttatat taaaaacttg actatttcat gaaaacaaaa
     2221 ttatatacta tattgtatac tataatacca ttactctgta ttgttgtttt gctttcatgc
     2281 acaacaaatg aaaagtcagc ggtgagcact atagtacatt ctatcaatgg acagcccgtt
     2341 attcctttta ctgctaacaa actatacatt gaaccattta aaaatgaaac aggcacacag
     2401 cactatgaag atagtataaa aaaaaggatt gttgaaaaga ttgttgcaaa cggcaggctg
     2461 gcgatagtta cttcagaaga ggccagcgat gtaaaactca tgggtacact acaatcattg
     2521 caaatacagc ccataaccat tgactatgcg gggatagtga aaaaaaagcg attattgatt
     2581 attgctgtga taagtttagt aaatactc
//
LOCUS       DAOSZP010000324         2603 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_30674_length_2603_cov_4.538854, whole genome shotgun sequence.
ACCESSION   DAOSZP010000324 DAOSZP010000000
VERSION     DAOSZP010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2603
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30674_length_2603_cov_4.538854"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2010
                     /locus_tag="PK348_07465"
     CDS             <1..2010
                     /locus_tag="PK348_07465"
                     /inference="COORDINATES: protein motif:HMM:NF014695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HXK66089.1"
                     /translation="LIIAGVYILYSLIQRVRIWRKGKPELRTDFHEKRIIALLKYVFL
                     QKKILREKYAGIMHASLFYGFVGLFIVTFIILIQEDVTELFFHFKFLVGDAYLLWSLA
                     GDVFGVAVIVGLVMAFWRRYKVKPSRLDTKPIDTFALVLIAFIILTGFLNESLRIAIT
                     GFPTFEVWSPVGYTLAHLFGWISIPTLEVTHYINWWLHMIGAFTFIGLIATDKLGHVL
                     ITMLNVYFQNLDNESPKTKFAMPIISPQEFETAESFGVGTVEHFTWKQLMDGDACTRC
                     GRCQDNCPAYLTEKPLSPKKIVNDVKAAMDDRIPKLLVAQDPASVESRALIGEYVLED
                     EIWSCTNCAACMENCPVQIEHVPKINDMRRYQVLMEGKMAPELQTSFSNMENNSNPYG
                     FAFAARGDWVTEDLGVKTLAEDPDVDFLYYVGCAASYDKRNQKVAIALCKILKAAGYK
                     VGILGAEEACCGDSAMRAGNEYLFHSLAAQNLETFKNYGVKKIITTCPHGYNMLKKEY
                     PKFAKVGVDSSGNPLEANYEVYHHTEVIMDLIKLGKIRLSKPLNETITYHDSCFLGRY
                     NEIYDQPRNIIKAIPGTNFVEMSRNHERSFCCGAGGARMWIEEHLGTRINQFRTKDAQ
                     ATGATKIGTACPFCLTMLSDGANELDIQNLQTFDLAEYVWEAMEK"
     gene            complement(2068..>2603)
                     /locus_tag="PK348_07470"
     CDS             complement(2068..>2603)
                     /locus_tag="PK348_07470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HXK66090.1"
                     /translation="EVYMPLYEINGRKPVIGEGTWIAPSAEIIGDVRIGKRCYIGFGA
                     IIRGDYGTIIIGDETAIEEGCMIHARPMQMVQIGNRVTVGHMVMIHGSIIMDNAVIGM
                     HATLSDNAEIGEWAIIAEHALVVNKQKIPGYKVYGGVPAKEIGDVLQKHMDIWNAGKQ
                     VYVDLTYQYPQSFKRID"
BASE COUNT          757 a          536 c          541 g          769 t
ORIGIN      
        1 ttaattatcg caggtgtata tattctgtac agtttaattc agagagtgag aatctggaga
       61 aagggtaaac cagaactaag aaccgatttt cacgaaaaaa ggattatcgc actcctcaaa
      121 tatgtctttt tacagaagaa aatactgagg gaaaaatatg caggcatcat gcatgcttcc
      181 ctgttctacg gatttgttgg gttgttcatt gtaacgttca tcatacttat acaggaagac
      241 gttactgaac tattcttcca ttttaaattt ttggtgggag atgcttatct attgtggtca
      301 ctggctggcg atgtgtttgg cgttgctgtt atcgttggac tggtcatggc tttctggcgc
      361 agatataaag tgaagccaag caggcttgat accaagccaa tcgacacatt tgcactggtg
      421 ctgattgcat ttattatcct caccggtttt cttaatgaat cactacgaat tgcaatcacc
      481 ggtttcccaa catttgaagt gtggtcacca gtgggctata cgctggctca tctttttgga
      541 tggatcagca tacctacact tgaggttacc cactatatta actggtggct tcacatgatt
      601 ggagctttta catttattgg gctgatagca actgataaat taggccatgt gcttattacc
      661 atgcttaatg tctacttcca gaatttagat aatgaaagcc caaaaacaaa gtttgcaatg
      721 ccaattatca gtccccagga gtttgaaaca gcagaatcat ttggtgtggg aaccgttgag
      781 cattttacct ggaaacagct tatggatggt gatgcatgta cccgttgtgg acgctgtcag
      841 gataattgcc ctgcatactt aactgaaaag ccactatctc ccaagaagat cgttaacgat
      901 gttaaagctg ctatggatga ccgtattcca aaattacttg ttgcccagga tccagcatca
      961 gttgagtcca gagcattaat tggcgagtat gtactggaag atgaaatctg gtcatgtact
     1021 aactgtgcag cctgtatgga aaattgcccg gttcagattg agcatgttcc aaaaataaat
     1081 gatatgcgcc gctatcaggt cctcatggaa ggcaagatgg ctcctgagtt gcagacctca
     1141 ttcagcaata tggaaaacaa ctccaatcct tacggatttg catttgcagc ccgtggtgat
     1201 tgggtcactg aagatttagg tgttaaaacg ttagctgaag atcctgatgt agacttcctc
     1261 tattatgttg gatgcgctgc atcatatgac aaacgcaatc agaaagtggc gatagctctt
     1321 tgcaaaatac tcaaggctgc aggatacaaa gttggtatcc ttggagccga agaagcatgt
     1381 tgcggtgact cagcaatgcg tgcaggaaat gaatatctgt tccattccct ggcagcacaa
     1441 aacttggaaa catttaaaaa ctatggtgta aaaaagatta tcactacatg tccacacgga
     1501 tacaacatgc tcaagaaaga atatcctaaa tttgctaaag taggtgttga ttcaagtggt
     1561 aatccattag aagccaatta tgaagtatat catcacacag aggttatcat ggatcttatt
     1621 aaattaggca agatccggct gtcaaaacct cttaatgaaa ctatcaccta tcatgattca
     1681 tgtttcttag gtcgatataa cgagatatat gatcagccac gaaatattat aaaagctata
     1741 cctggcacta actttgttga aatgagcaga aaccatgaac gaagcttctg ttgcggtgct
     1801 ggcggtgctc gtatgtggat tgaagagcat ttgggtaccc gtattaatca gttcagaaca
     1861 aaggatgctc aggctaccgg tgctacaaag attggtactg catgtccatt ctgccttacc
     1921 atgctttctg acggtgccaa cgaacttgac attcagaatc tgcagacatt tgatttagct
     1981 gaatatgtat gggaagcgat ggaaaagtaa gaaaatattc tgaatctaaa agtaaaaggc
     2041 gtcgttgtat agcgacgcct tttttattta atcaatccgc ttgaatgatt gagggtactg
     2101 ataggtcaga tcaacatata cctgcttgcc ggcattccat atatccatat gcttctgtaa
     2161 cacatctcct atctcttttg ctggtacacc accgtatact ttgtaaccgg gaattttttg
     2221 tttgtttact accagagcat gttcagcaat aatagcccat tcacctatct cagcattgtc
     2281 acttaatgtt gcatgcatcc caatcacagc attgtccatg atgatagagc catgtatcat
     2341 aaccatatgg ccaaccgtta cgcgattacc aatctgtacc atttgcatgg gacgagcatg
     2401 aatcatacat ccttcctcaa tggcggtttc atcaccaatt attatggtgc cataatcacc
     2461 ccgtataatt gccccaaagc ctatatagca gcgcttgcca attctgacat caccaatgat
     2521 ctctgctgaa ggagctatcc aggttccctc acctattact ggtttcctgc cattaatctc
     2581 atacagtggc atgtatacct cca
//
LOCUS       DAOSZP010000325         2597 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_30779_length_2597_cov_2.979544, whole genome shotgun sequence.
ACCESSION   DAOSZP010000325 DAOSZP010000000
VERSION     DAOSZP010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2597
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30779_length_2597_cov_2.979544"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1583)
                     /gene="carB"
                     /locus_tag="PK348_07475"
     CDS             complement(<1..1583)
                     /gene="carB"
                     /locus_tag="PK348_07475"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HXK66091.1"
                     /translation="MPKRSDIKKVMIIGSGPIIIGQACEFDYSGTQACKALKKLGYEI
                     VLVNSNPATIMTDPGMADVTYIEPLTVESLAKIIEKEKPDALLPNLGGQTGLNLSAEL
                     SRQGILQKYGVTIIGVEVDAIERGEDRIAFKNTMNALGIPMPQSEPAYSIDEAKRIAD
                     KLGFPLVIRPAYTLGGTGGGHVYNMEELETIVSRGLSASLIGQVLVEESVIGWEELEL
                     EVVRDAKNQMITVCFIENVDAMGIHTGDSFCTAPMLTISQETQKKLQEYSYKIVEAIK
                     VIGGTNIQFAHDPKTGRIVVIEINPRTSRSSALASKATGFPIAFVSSLLAAGLTLDEI
                     PYWRDGTLDKYTPSGDYVVVKFARWGFEKFKGVEDKLGTQMRSVGEVMSIGKNYKEAL
                     QKAIRSLEIGRYGLGFAKNYNNLDLDELIRLVSTPTSERQFIMYEALRKGADVDILHE
                     RTHIKKYFIEQMKELVELENQMLAYKGKSLPDTLLTTAKQWGFADRYLSKILDIPEDS
                     IRRQRVKLGVVERWEPVPV"
     gene            1997..2143
                     /locus_tag="PK348_07480"
     CDS             1997..2143
                     /locus_tag="PK348_07480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66092.1"
                     /translation="MEIMRISGADVIPAGFSVNNQQQEEPKAQTEVKKEPEKAKETTI
                     DTYA"
     gene            2156..>2597
                     /locus_tag="PK348_07485"
     CDS             2156..>2597
                     /locus_tag="PK348_07485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66093.1"
                     /translation="MPITKAEKAAYNDFIKGYKSKLDELTKQIKETEQKKKKMPNIKG
                     YVNIELILLYTKYIYTLLNMNKASLDMLKIKNERFLNDARKEFYRVLQLFEEIVGKDI
                     DRPLKENEEYLQAIAKLNPSQILNLIKELHTLLQKIMEALGESSK"
BASE COUNT          784 a          510 c          502 g          801 t
ORIGIN      
        1 cttacgggta ctggttccca gcgttctact acacccaatt taaccctctg ccttcggatt
       61 gaatcttcag gtatgtccag tatttttgaa agatacctgt cggcaaatcc ccactgtttg
      121 gctgtcgtga gcaatgtatc aggcagtgat tttcctttat atgcaagcat ctggttttcc
      181 agttcaacca gttctttcat ctgctctatg aaatattttt ttatgtgggt gcgttcatgc
      241 agtatatcta catccgcccc ttttctcagg gcttcgtaca ttataaactg gcgttcgctg
      301 gttggtgtgc tgacaagacg gatcagttca tcaaggtcaa gattgttgta atttttggca
      361 aaaccaagtc cgtatcggcc aatttcaagg gacctgattg ctttttgcaa tgcttccttg
      421 tagtttttcc ctatactcat aacttcgcct acagaacgca tctgtgtgcc aagcttatcc
      481 tctactccct tgaatttttc aaaaccccat cgtgcaaatt tgactaccac ataatctcct
      541 gaaggcgtat atttatcaag agtgccatca cgccagtagg gtatttcatc aagggttaac
      601 cctgctgcaa gaagtgatga tacaaatgct atagggaatc ctgttgcctt tgaggccagt
      661 gctgaagaac gtgacgtgcg aggattaatc tcaatgacca caatccttcc ggtttttgga
      721 tcgtgagcaa actggatgtt ggtgccgcct ataactttta ttgcttcaac aattttgtat
      781 gaatattcct gaagcttctt ctgtgtttcc tggcttatgg ttagcattgg tgcagtacaa
      841 aaggaatcgc cagtatggat gcccatcgcg tcaacatttt caataaagca tacagtaatc
      901 atctgattct ttgcatcacg cactacttca agttccagtt cttcccagcc aatgactgat
      961 tcttcaacaa gaacctggcc aatcaaactg gccgatagtc ctcgcgacac aatggtttca
     1021 agctcttcca tgttatatac atgaccacca ccggtacctc ccagcgtata ggctgggcga
     1081 ataaccagag gaaagcccaa tttgtcagca attctttttg cttcgtcaat actataggct
     1141 ggttcacttt gaggcatggg tatgcccagt gcattcatgg tatttttaaa tgcaatacgg
     1201 tcttctccac gctctatagc atcaacttca acgcctatta tggtaacacc gtatttttgc
     1261 aatatcccct gtcgggacag ttcagccgaa aggttaagcc ctgtttgacc acctaagtta
     1321 ggaagcaatg cgtcaggttt ttctttttca ataatttttg caagggattc aacggttaaa
     1381 ggttcaatat aggtgacatc tgccattcca ggatcggtca ttattgtggc aggatttgaa
     1441 tttaccagaa ctatctcata acctaatttt tttaatgcct tacatgcctg cgttcctgaa
     1501 taatcaaatt cacatgcttg accaataatt attgggcctg aacctattat cataactttt
     1561 ttaatatcac ttcgttttgg caagggattc ctccaatgat ttttgcatat ttgtgagata
     1621 tggattgata gtgtcaatta acattagatt tattgcaact ttattttttg cttgatattt
     1681 tatataagta taatgtagat atttatcaaa gcattattag tgacagagta tacaagaggt
     1741 gttgcacgca gggaacgcag agtaaatgaa ttaaagaggc tttatagcta tctattgacg
     1801 aatcatgtgg atatctaaaa ctgtggctat attaaagatt atagagtatt aaaagcaaga
     1861 tgggtgtgtc ccaaaagtcg tttcaataca aattttccaa cgctcattga ttaattttgc
     1921 cagattttgt ggcaaaattt actcaacgac ctcttttggg acagacccat gacatcctta
     1981 atggaggagg gatactatgg aaataatgcg aatttcaggt gcagatgtta ttcctgcggg
     2041 gttttcggtg aataatcagc aacaggaaga gccaaaagca cagactgaag ttaaaaaaga
     2101 acctgagaag gcaaaagaaa caactatcga cacttatgca tagagggaaa aatcaatgcc
     2161 aatcacaaaa gccgagaaag cagcgtacaa tgactttatt aaagggtata aatcaaaatt
     2221 agatgaattg accaaacaga taaaggaaac tgagcagaaa aagaaaaaaa tgcccaatat
     2281 taagggttat gttaatattg agcttatttt actgtataca aaatacatat atacattact
     2341 caatatgaac aaagcatcgc tggatatgct gaagattaaa aacgaacgtt ttttaaacga
     2401 tgcacgtaaa gagttttaca gagtgttgca attatttgaa gagatagttg gcaaggacat
     2461 tgaccgcccg ttaaaagaaa atgaggaata tttacaggca atagcaaaat taaatccttc
     2521 acagattctt aatttgatta aagaactaca taccttactg caaaagatta tggaagcttt
     2581 aggcgaaagc tctaaat
//
LOCUS       DAOSZP010000326         2597 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_30782_length_2597_cov_2.476397, whole genome shotgun sequence.
ACCESSION   DAOSZP010000326 DAOSZP010000000
VERSION     DAOSZP010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2597
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30782_length_2597_cov_2.476397"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /locus_tag="PK348_07490"
     CDS             <1..409
                     /locus_tag="PK348_07490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66094.1"
                     /translation="NRALKDKKDIHVYSAIALLHHIYKKFESGKGASSFQELLPMTLN
                     DVDLLDMLLDLFIKEKFITHTEEFGIIPANASKNISIAKIIDTIYSISLDIPVTKQDK
                     LKTYLADLFKDMKQSLDSIVKNKTLADVIQATE"
     gene            complement(420..1301)
                     /locus_tag="PK348_07495"
     CDS             complement(420..1301)
                     /locus_tag="PK348_07495"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HXK66095.1"
                     /translation="MERAEIRFDLVLLVSFFMHTIVIVSFLIAQIRYGAFAKQQADSL
                     FKGRDIIVNINQDDVKDINKNTLLSDKTSKAKGYITKEKGDRWLNNSLDFKMQKGVRQ
                     LGKGGIASSTAAKDKIMVAQENTEYSITFFKDEAGGVPEFEGDKEFTRIPDKYSITPK
                     NALFFTSDGFFSFNTVKFKPFEYFKNMKDKVAANWHPPLLANAVIYGYNPMTGSMAPG
                     RMRIMAVPTQDVKLYFTMDREGNVLDIVLVDSLGNKAIDESCIDAIRLSKNFGKVPDE
                     IQGKVIVIPFLFRIVSQ"
     gene            complement(1283..2533)
                     /locus_tag="PK348_07500"
     CDS             complement(1283..2533)
                     /locus_tag="PK348_07500"
                     /inference="COORDINATES: protein motif:HMM:TIGR03991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sugar nucleotidyl transferase"
                     /protein_id="HXK66096.1"
                     /translation="MKIIIFEDSQYNDFFPVTLTRPVWDIRCGIFTIHYRYATLFPSQ
                     FPDSIYFYTRHHLEAIAHMQYPQLHINEPVKMNHSEELLFINARVIYPELSDLHDNTI
                     YMHNGIPVLAKLHEAAIPQFTTTEDVQNFLQNKAVVENYKGYVLTSLWELIGNNGDII
                     TQDFKLIKNPRGNNSVTILGDSSQLIVEDDVTIEPYVVIDCTKGPVYIGKGCSIKSFT
                     RIEGPCAIADNTILLQAKVREGCSIGPWCRVGGEVEECIFQGFANKYHEGFIGHAYIG
                     SWVNLGAMTTNSDLKNNYTTVKLFIGEEKINTGLLKVGCFIGDYTMTSIGTLINTGTV
                     MGPGCMVIHNGAITPKFIPPFSWYIDGKIVDLPSEDKFFATCKTMMSRRNVEFTAEHR
                     KLLEEVRSQTKIYRKGIGRWNVPK"
BASE COUNT          791 a          527 c          417 g          862 t
ORIGIN      
        1 aaacagggca ttgaaggata agaaggatat tcatgtttat tcagctatag ccctgttaca
       61 tcatatatat aaaaaatttg aatcgggaaa aggcgcaagc agttttcagg aattgctgcc
      121 aatgacgctg aacgatgttg acctgcttga tatgctgtta gaccttttta tcaaagaaaa
      181 gtttataaca cacactgaag aatttggaat cattccggca aatgcaagca aaaacatcag
      241 catagctaaa attattgata caatttacag catatcactg gatatcccgg ttaccaaaca
      301 ggataaatta aaaacgtatt tagctgattt attcaaggat atgaagcaat cactggattc
      361 tattgtcaaa aataaaacgt tagctgatgt tatacaagca acagaatgaa gtgtattgac
      421 tattgcgaaa ctatcctgaa taaaaatggg attactatta ctttgccctg aatttcatca
      481 ggtactttgc caaagttttt tgacaaccgt atggcatcaa tgcaggattc atcaatagct
      541 ttattcccca acgaatcaac cagaacaatg tcaagaacgt taccttcacg atccatggta
      601 aaatacaatt taacatcctg agtaggcact gccattattc tcattcttcc cggtgccata
      661 cttcctgtca ttggattata tccatatatt actgcattag caagaagtgg tgggtgccag
      721 ttggcagcca ctttatcttt catatttttg aagtattcaa aaggtttgaa ttttactgta
      781 ttgaaagaaa aaaatccatc actggtaaaa aacagcgcat ttttgggtgt tatgctatat
      841 ttgtcgggta tgcgtgtaaa ttccttatcg ccttcaaatt caggtactcc acctgcttca
      901 tctttaaaaa atgtaatgct gtattccgta ttttcctgtg caaccatgat tttatcttta
      961 gcagctgtgc tgcttgcaat cccaccttta cctaactgcc gcaccccttt ctgcatctta
     1021 aaatccagag agttgttgag ccacctatcg cctttttctt ttgttatata gcctttggct
     1081 tttgacgtct tatctgaaag aagtgtattt ttatttatat cttttacatc atcctgattg
     1141 atgttaacaa tgatatccct gcctttaaac aatgaatcgg cttgctgttt tgcaaacgca
     1201 ccataccgta tctgtgcaat taaaaaagaa acaatcacaa tagtatgcat gaaaaacgaa
     1261 accagaagta caaggtcaaa tcttatttcg gcacgttcca tcttccaatc ccttttctgt
     1321 atatttttgt ttgactacgc acctcttcca gcagtttacg atgttcagca gtaaactcca
     1381 cattgcgacg tgacatcatt gttttgcatg ttgcaaaaaa tttatcctcc gaaggcaggt
     1441 caacaatctt accatcaata taccagctga atggcggtat aaactttggt gtgattgcac
     1501 cattgtgaat gaccatacaa cccggcccca tgacagtacc ggtgttgatt aaggtcccaa
     1561 tacttgtcat agtgtaatca cctataaaac atccaacctt aagcaatcct gtgtttatct
     1621 tctcttctcc tataaataat ttaacggttg tatagttatt tttcaaatca ctgttggtag
     1681 tcattgcccc taaatttacc cagctaccaa tatatgcatg acctataaat ccttcatgat
     1741 atttatttgc aaacccttga aaaatacatt cttctacttc accaccaacc ctgcaccaag
     1801 gcccaatgct gcaaccttct ctcacttttg cctgtaatag tattgtatta tctgcaattg
     1861 cgcatggtcc ttcaattctt gtaaaagatt ttattgagca accctttcca atatacacag
     1921 gtccttttgt acaatctata actacatacg gttctattgt tacatcatct tctactatta
     1981 attgggaact atcgcctaaa attgttacac tattattccc cctgggattt tttattaact
     2041 taaaatcctg tgttataata tccccattgt taccaatcaa ttcccacaaa gatgtcagca
     2101 cataaccctt atagttttct actacagctt tgttctgtaa aaaattttgt acatcctcgg
     2161 tggtggtgaa ttgtggaatg gcagcttcat gtaactttgc aagtacagga ataccgttat
     2221 gcatataaat ggtattatca tgtaggtccg atagctccgg gtatataacc cgtgcattga
     2281 taaacagcaa ttcttctgaa tggttcattt tcaccggttc attaatatgt aactgtggat
     2341 actgcatatg agctatcgct tctaaatgat ggcgtgtata aaaatagatt gagtcaggaa
     2401 actgtgatgg gaataatgtg gcatatcgat agtgtatcgt gaaaattcca cagcgaatat
     2461 cccataccgg ccttgtaagg gttactggaa aaaagtcatt gtactgtgaa tcttcaaaaa
     2521 ttattatttt catgttttct ttgtaacagt tgcattaata ttaaatcatt aatcttcaaa
     2581 ccatctattc ataatta
//
LOCUS       DAOSZP010000327         2592 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_30839_length_2592_cov_3.759559, whole genome shotgun sequence.
ACCESSION   DAOSZP010000327 DAOSZP010000000
VERSION     DAOSZP010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2592
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30839_length_2592_cov_3.759559"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="PK348_07505"
     CDS             <1..375
                     /locus_tag="PK348_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013843009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HXK66097.1"
                     /translation="MTGGQMAPTTLLGQQTTTTPYGRNFRSEGYPIRMAEMLATLEGV
                     AYSARVALHKPKYVMQAKKAVEKAFMMQINEMGFSVVEMLSTCNTNWRVSPTEAIDKL
                     ENEMIPYFPLGVFKERTGKDFL"
     gene            406..942
                     /locus_tag="PK348_07510"
     CDS             406..942
                     /locus_tag="PK348_07510"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HXK66098.1"
                     /translation="MYYDMIMAGFGGQGIQIISQLVAVAAINKGYEVTYLPSYGVEKR
                     GGRTNVFVVISDEEIGSPITNHPYALLVMDQIAYDTYIHILRPDGLLVANESLIDTTT
                     HRRNDIVSCCLFCNEEAVKLGNAKLANMIALGALLKNSNVLTVDDIEKVIDQVIPERL
                     AHTIPANMQAIRHGYKLA"
     gene            complement(962..1402)
                     /locus_tag="PK348_07515"
     CDS             complement(962..1402)
                     /locus_tag="PK348_07515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HXK66099.1"
                     /translation="MGKKKEKITQEIVVVLNTNIVVSAILFKGKVSTLMALWHNGTIK
                     PVMTKEIFDEIHAVLKYPKFMLTKHEIDYILNEELLLFFDVITDNYPNISVCKDEDDD
                     KFIRCALSAKAKYIITGDKELLQIKKYKDIEIMSCNQFLNLFKK"
     gene            complement(1378..1614)
                     /locus_tag="PK348_07520"
     CDS             complement(1378..1614)
                     /locus_tag="PK348_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HXK66100.1"
                     /translation="MVAKKTSKNQLTLPAKIVKEFPDIDYFDITVKDNKIIMVPVVLK
                     PQTGINTIREKMKKLGINGKDIEEAITWARKKKK"
     gene            complement(1705..>2592)
                     /locus_tag="PK348_07525"
     CDS             complement(1705..>2592)
                     /locus_tag="PK348_07525"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HXK66101.1"
                     /translation="HMEIALAESEKRYRHLVNRIPAAVVEMNVNGDIVFVNDYFVHLT
                     GYTLDNLKGTHWFRSILHPHEDKRKIRQFVELIQQGDVSNFEIRIINREGHDTHITIT
                     TSNVYNAVGKLERIVCIATDITPIVMLRDKLKELAIKDELTGLYNRRGFTMLAEQQMK
                     LSKRSGKGIALFYIDMDNMKTINDTMGHHEGDKALIDVAGILRLSFRDSDIIGRLGGD
                     EFAVLAIDCEKHFMEMMQQRLLDNAEEFNKKGLRMYTISLSVGSIFIGDSSSSLDEVL
                     SLADSLMYKNKMQKKQHRL"
BASE COUNT          799 a          503 c          430 g          860 t
ORIGIN      
        1 atgacaggcg gccagatggc accaacaacg ttgcttggcc agcaaacaac aacaacaccg
       61 tatggacgaa atttccgcag tgaaggctat cccattcgta tggctgaaat gcttgcaacg
      121 ctggaaggtg ttgcatattc ggcccgtgtc gcattgcaca agccaaaata tgtcatgcag
      181 gcaaaaaaag ctgtggaaaa agctttcatg atgcagataa atgaaatggg gttttccgtt
      241 gttgagatgc tttcaacctg caacaccaac tggcgcgttt caccaacaga ggcaattgat
      301 aaacttgaaa atgaaatgat accatatttt ccgctgggcg tcttcaaaga acgtacggga
      361 aaagattttc tgtaaaccga tagttactgt tgaaggagtt attcaatgta ttacgacatg
      421 attatggcag gttttggcgg acagggtatt cagattataa gccagcttgt tgccgttgct
      481 gccatcaata aaggctacga ggttacatac cttccatcgt atggcgttga aaaacgtggc
      541 ggacgcacca atgtttttgt ggtaatttct gatgaagaga taggatcacc cattaccaat
      601 catccatatg cattacttgt tatggatcag attgcgtatg atacctatat tcatatattg
      661 aggcctgacg gtttgcttgt tgcaaacgaa agcctcattg atacaaccac acatcggcgc
      721 aatgatattg tatcgtgctg tttgttttgc aatgaagagg ctgttaaact gggcaatgcc
      781 aaattagcta atatgattgc attgggtgca ttattaaaaa acagtaatgt gcttacggta
      841 gatgacatag aaaaggtaat tgaccaggta atccctgaac gcctggcaca taccattcct
      901 gcaaacatgc aggcaatacg ccatggatat aaattagctt aaaacaatca tacaatatac
      961 attacttttt aaataaattt aaaaattggt tacatgacat tatttctata tccttatatt
     1021 tttttatctg caacaattct ttatcaccgg taataatata ttttgcctta gctgataaag
     1081 cacaacgtat aaatttatca tcatcttcat ctttacaaac cgaaatattg ggataattat
     1141 ctgtaatcac atcaaagaaa agtaataatt cttcattaag aatataatct atttcatgct
     1201 ttgtaagcat aaattttgga tacttcaaaa cagcatgtat ttcatcaaaa atttctttcg
     1261 tcataacagg ctttatagtg ccattatgcc ataatgccat taacgttgaa actttgccct
     1321 taaaaagaat tgcagatact acaatatttg tattaagaac tacaacaatc tcctgggtta
     1381 ttttttcttt tttcttgccc acgttatagc ttcctcaata tctttcccat taatacctaa
     1441 cttcttcatt ttttctctta tagtattaat gcctgtctga ggtttcagaa caacaggaac
     1501 cattattatt ttattatctt tgacggttat atcgaaataa tcaatatcag ggaattcttt
     1561 tactatttta gctggtaatg ttaactgatt ttttgatgtt ttttttgcaa ccattcctta
     1621 cttccttaca gtaaggatat aataccttat cataatattt tatcaataaa tattttttat
     1681 aaaaaactat aaattttttt ggctttacaa gcggtgctgt tttttctgca ttttattttt
     1741 atacattaaa gaatcagcca acgataatac ctcatctaaa gatgaagaac tgtcgccaat
     1801 aaatatactt cccacactga gtgaaatagt atacatgcga agacctttct tattaaattc
     1861 ttcagcatta tcaagcaatc gttgctgcat catttccata aaatgctttt cacagtcaat
     1921 tgcaagaact gcaaattcat caccacctaa ccttccaatg atatctgaat ccctgaacga
     1981 taaacgcaaa attccagcca catcaatcag tgctttatcg ccttcgtggt gtcccatggt
     2041 atcattgatt gtcttcatat tgtccatatc aatatagaaa agtgctatgc ccttgccgct
     2101 gcgtttgctt aatttcattt gctgctcagc taacatggta aatccacgac ggttatacag
     2161 gcctgtaagt tcatctttaa ttgccagttc tttaagctta tcccgtaaca tgacaatggg
     2221 cgtaatatct gtggcaatac acactatacg ttctaatttt cccactgcat tatatacatt
     2281 tgatgtagtt atggttatat gagtatcatg gccttctcta tttattatac gtatttcaaa
     2341 gttgctaaca tcaccctgct gtatgagttc aacaaactgg cgtatttttc ttttatcctc
     2401 atgaggatgg aggatgcttc taaaccagtg agtaccttta aggttatcca gtgtatatcc
     2461 tgtcaaatga acaaaatagt catttacaaa tacaatatca ccgttgacat tcatttcaac
     2521 caccgctgca ggaattcgat taaccagatg gcggtatcgt ttttcagatt cagctaaggc
     2581 aatttccatg tg
//
LOCUS       DAOSZP010000328         2590 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_30866_length_2590_cov_4.276529, whole genome shotgun sequence.
ACCESSION   DAOSZP010000328 DAOSZP010000000
VERSION     DAOSZP010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2590
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30866_length_2590_cov_4.276529"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            182..499
                     /locus_tag="PK348_07530"
     CDS             182..499
                     /locus_tag="PK348_07530"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HXK66102.1"
                     /translation="MQKKECRRCGACCFVDMIAYATEDDFVRWRAEGRHDILHIIENE
                     ASIWAGDHLISSRNGHSIQRCPFLIMDDDGLYSCTIYNTRPAVCRDFEPGVSRMCPQY
                     EDQ"
     gene            534..1463
                     /locus_tag="PK348_07535"
     CDS             534..1463
                     /locus_tag="PK348_07535"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HXK66103.1"
                     /translation="MYKNWAVTLIIANGIIFLIQLLLSNTLVPYTVANNVVPMDIVTY
                     YLGLTPYLVVDKFYLWQLFSYMFLHSTSGFAHIIFNMYALFIFGMPVERLWGSKKFLI
                     YYLTCGIGAGLAIFIIALVSGGLGYYIPTIGASGAVFGLLLAFGILFPDVEILLFFII
                     PVKARTLVILYGALELFLELSGGFDNISHIGHLGGLATGILYFIITRKHAIRYKTRGF
                     VALQKKKATIPAEHVVDKKEIELQKTIVQKLYKQGEQALTDDEVQYIRYKEIMVETNT
                     VDCDISIFTSNSCENCIDRDACFVKIVKDLLKQ"
     gene            1656..>2590
                     /locus_tag="PK348_07540"
     CDS             1656..>2590
                     /locus_tag="PK348_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004357223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HXK66104.1"
                     /translation="MAKRVYLFGGNVTEGTADMKNLLGGKGANLAEMARIGIPVPAGF
                     TITTEVCNEYYKNNQKMPDGLKEEVIAALRKVEEIMGTKFGDPSNPLLVSVRSGARAS
                     MPGMMDTILNLGINEDVVKGLVTKTGNERFAWDSYRRFIQMYGDVVLGLKPESKDEHD
                     PFEVIMDELKEKRKVKNDLELTVDDLKELVARFKKIIKDKLNVSFPDDPYEQLWGAIG
                     AVFRSWNNERAILYRKMNNIDDSWGTAVNVQAMVFGNMGNDCATGVAFTRDPATGAKE
                     FFGEYLINAQGEDVVAGIRTPQQITLEGSRRWARE"
BASE COUNT          816 a          378 c          587 g          809 t
ORIGIN      
        1 aaagggatta ttcagaaaag tagatgagaa aattgctttg aatcttattg cttcaatgat
       61 cgttagaaca cggttatggt tttctccgga agaatggatg acaatagatg atttaattga
      121 atttatctac cagtttgcgt tgaaagggtt gtcataggca aactataata atttaaatac
      181 tatgcaaaaa aaagagtgca ggcgatgtgg tgcatgttgc tttgttgata tgattgcata
      241 tgcaactgaa gatgattttg tacgctggcg cgctgaaggg cggcatgata ttttgcatat
      301 aattgaaaat gaagccagca tatgggctgg tgatcatttg atttcttccc gtaatgggca
      361 ttcaattcaa cgatgtcctt ttttaataat ggatgatgat ggcctctata gttgtaccat
      421 ttataacaca cgaccggcag tttgcaggga ttttgagcct ggcgtttcaa gaatgtgccc
      481 tcaatatgag gatcaataat aaaattacgt aatagaaaga gcaggattgg ttaatgtata
      541 agaactgggc tgttaccctt ataatagcaa atggtataat ttttctcatt caacttttgt
      601 tgagtaatac tcttgttccg tatactgttg ctaataatgt tgtacctatg gatattgtta
      661 cgtattacct gggtttaact ccttatctgg tagttgataa attttatcta tggcagctat
      721 tttcgtatat gtttctccat agcacttcag gttttgcaca cattatattt aatatgtatg
      781 ctctttttat ctttggtatg cctgttgaaa gactgtgggg gagcaaaaaa ttccttatat
      841 actatttaac ctgtggtatt ggggcaggct tagcaatttt tatcatagct ttagtatcgg
      901 gtggactggg atattatatc ccaactattg gtgcttctgg agcagttttt gggcttcttc
      961 tggcctttgg catattgttt cctgatgttg aaatattact tttttttatc ataccggtga
     1021 aagcacgaac actggtgata ttgtatggcg ctctggaact gtttctggag ctatcaggtg
     1081 gatttgataa catctcacat atagggcatc tgggtgggct tgccactggt attctctatt
     1141 tcataattac ccgcaaacat gctatcagat ataaaaccag agggtttgtt gctttgcaaa
     1201 agaaaaaggc aaccattcca gctgaacatg ttgtagacaa aaaggagatt gaattacaaa
     1261 aaactatcgt acaaaaatta tacaaacagg gtgagcaagc gttaacagat gatgaagtgc
     1321 agtatatacg gtataaagaa attatggttg aaacaaatac tgtagattgc gatatcagta
     1381 tttttacgtc aaatagttgt gaaaattgta ttgatagaga cgcatgtttt gttaaaattg
     1441 taaaagattt gctcaaacaa tgatatatat cttgaaaaag atgatagtat tttgaaatta
     1501 atctttacaa cagaaatgta gtactataag gatgttaaac cggtaagtat tgctggtaaa
     1561 aatagcctgc tattctgtat ttattttgga aatgagatat gttaccattt ttatggtggc
     1621 aaaataataa atctaaataa gaagggagaa caacaatggc aaagcgtgta tatctgtttg
     1681 gtggtaacgt tacagaggga actgctgata tgaagaacct tttaggtggt aaaggtgcta
     1741 atttagcaga aatggcacgt attggcatac ctgttcccgc aggctttaca attacaacag
     1801 aagtatgtaa tgaatattat aaaaacaatc agaaaatgcc tgatggacta aaagaggaag
     1861 ttatagctgc attacgaaag gtagaagaaa ttatgggcac taagtttggt gacccttcca
     1921 accctttgct ggtgtcagtt agatctggtg cacgggcatc tatgcctgga atgatggata
     1981 caatcctgaa tttaggaata aatgaagacg tggtaaaagg ccttgtaaca aaaacaggaa
     2041 atgagcggtt tgcatgggat tcttaccgcc gctttatcca gatgtatggt gacgttgtac
     2101 tgggattgaa gccggaaagc aaggatgagc atgatccatt tgaagtaatc atggatgaac
     2161 ttaaggaaaa aaggaaagtt aaaaatgacc ttgagcttac tgttgatgat ttaaaagaac
     2221 tggtggcgcg atttaagaaa attataaaag ataagctcaa tgtatcattc cctgatgacc
     2281 cctatgagca gttatgggga gcaataggag cagttttcag atcatggaat aatgagcgtg
     2341 ctatcctgta ccgaaaaatg aataacattg atgatagctg gggtactgca gtaaatgttc
     2401 aggctatggt ttttggaaat atgggcaatg attgtgcaac cggtgttgcc ttcacaagag
     2461 atcctgctac aggtgcaaag gaattttttg gtgaatacct gattaatgct cagggtgagg
     2521 atgttgttgc aggcataaga acaccacaac agataacact tgaagggtcc cgtcggtggg
     2581 ccagggaaaa
//
LOCUS       DAOSZP010000329         2579 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_31008_length_2579_cov_3.680666, whole genome shotgun sequence.
ACCESSION   DAOSZP010000329 DAOSZP010000000
VERSION     DAOSZP010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2579
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31008_length_2579_cov_3.680666"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="PK348_07545"
     CDS             <1..196
                     /locus_tag="PK348_07545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66105.1"
                     /translation="LIVYIFSYVIRVKIFFLIKKIISLCSQRTLCEIKEKHHAKIAGV
                     DGKSGKHLWLIDNNDTLIKQ"
     gene            428..1582
                     /locus_tag="PK348_07550"
     CDS             428..1582
                     /locus_tag="PK348_07550"
                     /inference="COORDINATES: protein motif:HMM:NF017743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HXK66106.1"
                     /translation="MRKTWDKSGKNTMTHTLTIQRYANNGYGIGFIDGKTVFVPYSAV
                     GDTVAVTITRQAKNYCFASITDIITPSPNRITPQCPAFTHCGGCDFLHIPYKEELTVK
                     KELFINTLTHIGGLPHHILPAIELISGERFHYRSHATLQSDGTHIGFYKKDSHSVEPI
                     PAKGCLLLHEKLNTFILSKSCDSRANEPIKIAIDAQGSICSAPTAVITELECGITYER
                     SVSTFFQANKFLRSKMLLVVDELLQSFASILDVGCGVGFFSIYLARRMKGTGIDTNIQ
                     SIEWARYNAKRNNVTVDFHTVSIENYHPFKHTHECIIIDPPRQGISKRGRKTIVAMNP
                     SCIIYVSCNPVTFARDIKSFVDSNYRLQKLYMIDMFPCTHHTEVIGLLTK"
     gene            complement(1584..2138)
                     /locus_tag="PK348_07555"
     CDS             complement(1584..2138)
                     /locus_tag="PK348_07555"
                     /inference="COORDINATES: protein motif:HMM:NF024503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HXK66107.1"
                     /translation="MPHKNRNIVYYAFFISIAVHGVFLCVSFGGRTTDIQKHTLNVTM
                     HTYTILPHVEHAADTTRLKNNPAKPVKEGGVMASQYMVSNEDSQTVTMQYTDMIRQRI
                     QESLVYPATARKDGIEGKTYIKFTIDKNGNLLSAIMVRSSGSALLDDAALKAIHSASP
                     FPAIPDTIGRERMTFVQGLTFTLK"
     gene            complement(2122..>2579)
                     /locus_tag="PK348_07560"
     CDS             complement(2122..>2579)
                     /locus_tag="PK348_07560"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HXK66108.1"
                     /translation="QRYIRGNKAMSAERLFIERRKKVVIDLNIAPLIDVVFQLLLFFA
                     LTSYYVANPGIEVSLPKANSAVTVQRNNIEIYITGSNAIYCNATKVELSALKEMLSTL
                     SAASKTVILKADKTVQLGMVVQVIDIIKQTQVKDLVIATSMEENNASQK"
BASE COUNT          790 a          516 c          483 g          790 t
ORIGIN      
        1 actaatagta tatatttttt catatgttat tcgtgtaaaa attttttttc ttattaaaaa
       61 aataatatca ctgtgctctc agcgtactct gtgtgagatt aaagaaaagc atcacgccaa
      121 gatcgcagga gtcgatggga aaagtggaaa gcatttatgg ctaattgata ataatgatac
      181 tttaattaaa caataaaatc aaccatcgca ggcgctcaag cgtttgagcg cctgcgtaat
      241 gagcaccaga gctaaaaaga aatatagcat tgcaataatt tcataacaaa taaaaccctg
      301 aaatgacttc atttgtatga tacaatgggg tcagagccat atatggtatc atttctgtgt
      361 cctcagcgat ctctacgtga gattaaagca ataatgtatc acgataagat cgcagggagc
      421 accgagtgtg cggaaaacgt gggataaaag tggtaaaaat actatgacac atactctcac
      481 catacagcga tatgccaaca atggctatgg aattggtttt attgatggca aaaccgtatt
      541 tgttccctac agtgctgtgg gtgatactgt tgccgttacc ataactcgcc aggcaaaaaa
      601 ctactgtttt gcttcaatta ccgatattat aacgccttca cccaatcgta ttaccccaca
      661 atgtcctgca tttactcact gtggaggatg tgactttctt catattccct acaaagagga
      721 acttacagta aaaaaagagt tattcataaa tacattaaca cacattggtg gtcttcctca
      781 ccatatcctg ccagcaattg aattaatttc aggtgaacga tttcattacc gaagccacgc
      841 tacacttcaa tcagatggta ctcatatagg cttttataaa aaagattcgc atagtgttga
      901 accaatacct gcaaagggtt gcttacttct tcatgaaaag ctaaatacat ttatcctcag
      961 caaatcgtgt gatagccgcg ctaacgaacc tatcaaaatt gctatcgatg cacagggctc
     1021 aatatgctct gcccctactg cagttatcac tgaactggaa tgtggcatca cttatgaacg
     1081 aagtgtcagt acattttttc aggcaaacaa attcctgaga agcaagatgc ttcttgttgt
     1141 tgatgaacta ttgcaaagct ttgcatcaat tcttgatgtg gggtgtggtg ttggcttttt
     1201 tagtatttat ttagccagaa gaatgaaagg cactggcatt gatactaata ttcagagtat
     1261 tgaatgggca aggtataatg caaaacgtaa taatgttact gttgattttc atacggtaag
     1321 tattgaaaac tatcaccctt ttaaacatac tcatgaatgt attattattg acccacctcg
     1381 tcagggcata tcaaagcgag gaagaaagac cattgttgcc atgaatcctt cgtgcattat
     1441 atatgtttcc tgcaatcctg taacctttgc ccgtgatatc aaaagctttg ttgacagtaa
     1501 ctatcgctta caaaaattat atatgattga tatgttccca tgcacccatc acaccgaagt
     1561 gattgggctt ttgacgaagt agactacttc aacgtgaacg tcaacccctg cacaaacgtc
     1621 atgcgctctc tgccaatagt atcaggaata gctgggaagg gtgaagcgct gtgtattgcc
     1681 ttaagcgcag catcatctaa aagtgctgac cctgaagaac gtaccatgat agccgacagt
     1741 agattgccat ttttatcgat ggtgaattta atataggttt ttccttcaat accgtcctta
     1801 cgtgctgtag ccggatatac taacgattcc tgaatacgct gtcgtatcat atcggtatat
     1861 tgcatggtta ctgtctggct gtcctcattg gaaaccatgt attgcgatgc catgacacca
     1921 ccctctttaa caggctttgc aggattattc ttgagcctgg ttgtatcagc agcatgttca
     1981 acatgtggca atattgtata tgtgtgcatg gtaacattca atgtatgttt ttgaatatcc
     2041 gttgtcctgc ctccaaatga cacacacagg aaaacaccat gcacagcaat cgaaatgaag
     2101 aatgcataat aaacaatgtt tctatttttg tgaggcattg ttttcttcca tgcttgttgc
     2161 tatcaccagg tctttaacct gtgtttgttt tatgatatca atcacctgca ccaccatgcc
     2221 aagttgtacc gttttatcag ccttcaggat aacagtcttt gaagcagctg acagtgtgga
     2281 aagcatttct ttaagtgccg atagttcaac ctttgtggca ttgcaataaa tggcattact
     2341 accggtaata tatatttcaa tattgttcct ttgcacagta acagccgaat tagctttggg
     2401 taatgatact tcaataccag gattggcaac ataatatgat gtgagtgcaa aaaaaagcaa
     2461 tagctggaac acaacatcaa taagcggtgc tatattcaaa tcaatcacta ctttctttct
     2521 tcgttcaata aaaagccttt cagcactcat tgctttatta cctctaatat atcgctgat
//
LOCUS       DAOSZP010000330         2578 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_31032_length_2578_cov_2.720571, whole genome shotgun sequence.
ACCESSION   DAOSZP010000330 DAOSZP010000000
VERSION     DAOSZP010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2578
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31032_length_2578_cov_2.720571"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..617
                     /locus_tag="PK348_07565"
     CDS             <1..617
                     /locus_tag="PK348_07565"
                     /inference="COORDINATES: protein motif:HMM:NF027439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003860 - 3-hydroxyisobutyryl-CoA
                     hydrolase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HXK66109.1"
                     /translation="DVGSTGWLFTKCKKGYPYYLALTGDTLYGAECVHAGLAHNQILS
                     HIVSQCIAELERLQCSNDEVLSRINSILSFYSTPVQLSQGAKDEWIGQYFDTDKPVQC
                     MLQSLKQCSTHHKLCNGVFTRIAERSPTSLVLTHLLLKVNKGKPLDEVFARELKAAIF
                     MTQHNDYREGVRARLIDKDNKPEWQPEKIEDVDSALLQKVISSN"
     gene            complement(614..1741)
                     /locus_tag="PK348_07570"
     CDS             complement(614..1741)
                     /locus_tag="PK348_07570"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HXK66110.1"
                     /translation="MSIPRPNHSDTLDTVARYIQDILAQHNIPFELQHVTIYPYNMLI
                     AGLVCFILALLFAWCIKKQKSLLALVFLVAIPFILICEFELVKPVVSWIVPKESANII
                     VSFPQHHATHTLVLVAHYDSKTDIFDHIERAKIYQFIVPSILAGLLVLLWLALSKKIT
                     LLTKPLYRRIAAIIVLLCIIEWLLVAVAMGGFIFLKQQSYGAIDNASSVVALLGLADN
                     IKQGTVVNKSLNVEIVFTTGEEINLQGAKWYVKKLLSNKKPQELSVINLELVGEPGNM
                     VYWEKAGVMLTFYSADSNLIQRLAKTYRTVTKKDVGTLPKLTDDSIMFMREGIPAITV
                     GFANETYGVSGLHSTKDNLQRVSMDNVHTMVKTLGEIITHF"
     gene            complement(1783..2256)
                     /locus_tag="PK348_07575"
     CDS             complement(1783..2256)
                     /locus_tag="PK348_07575"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HXK66111.1"
                     /translation="MNIAIAIEKKYAKLNSYKEMEYAPYRDRWQTRFWLAAQYGKENN
                     NGNLPTAIYTKNNSEAKKLMDQVLDCTFRDANGTTLLMIAAYTDNVEIASLLLAKKCN
                     INARDNHGCTPLMYAVFGNAVHTMELLLKNNANVMMESPLLLKLKNYIIVALVNN"
BASE COUNT          792 a          501 c          498 g          787 t
ORIGIN      
        1 ctgatgtggg gtcaacgggg tggcttttta ctaaatgtaa aaaaggctat ccatactatt
       61 tagcactcac gggcgatact ctctatggtg cagaatgcgt ccatgctggt ctggcacata
      121 atcagatact atcgcacata gtatcacaat gtattgctga gcttgaacgt ttgcagtgca
      181 gcaacgatga ggtactttca agaattaata gcatactttc tttttatagt acacctgtac
      241 aactgagtca aggggcaaaa gatgaatgga ttggacagta ctttgacact gataagccag
      301 ttcaatgtat gttacaatca ctgaaacaat gctccacaca ccacaagctg tgcaatggcg
      361 tttttacacg cattgccgag cgttccccca catcacttgt cctgacccat ctattgctga
      421 aagtaaacaa aggcaaaccc cttgatgaag tttttgcacg tgaactaaaa gctgccattt
      481 ttatgacgca gcataatgat taccgtgaag gggtcagagc caggttaata gataaagaca
      541 ataaaccaga gtggcagcct gaaaaaattg aagatgttga tagtgcgtta ctacagaagg
      601 taattagcag taattaaaaa tgagtaatga tctcgcctag cgtctttacc attgtgtgga
      661 cattatccat tgacacacgc tgcaggttgt ctttggtgct gtgcaggcca ctcacaccat
      721 acgtctcatt tgcaaaacca acggtgatag cgggtatacc ttcgcgcata aacatgatgg
      781 aatcgtcagt gagttttggt aatgtgccaa catctttttt tgtaaccgta cggtaagttt
      841 ttgcgagcct ttgaatcaga ttgctatcgg cactataaaa tgtaagcatt actccggctt
      901 tttcccagta caccatgtta ccaggctccc caacaagttc taaatttatg accgatagct
      961 cctgtggctt tttattggat aaaagttttt taacatacca tttagccccc tgcagattaa
     1021 tttcttcacc agttgtgaac acaatctcca cgttaagcga tttattgacc acagtacctt
     1081 gcttgatatt atcagcaagc cctaaaaggg caaccacgga agaagcgttg tcgatagctc
     1141 cgtatgattg ctgttttaaa aatataaatc cacccatagc aacagctact aaaagccact
     1201 caatgataca taacagcaca attattgcag caatgcgcct atataatggc tttgttaata
     1261 atgtaatttt ttttgataag gccaaccaaa gcagcacaag taatcctgcc agaatgcttg
     1321 gaacaataaa ctggtatatt ttggctcgct ctatatggtc aaatatatca gttttggaat
     1381 catagtgtgc aacaagaacc agcgtgtgtg ttgcgtgatg ttgtggaaat gaaacaatta
     1441 tatttgcact ttcttttggg acaatccacg aaacaactgg tttcacaagt tcaaactcac
     1501 agataaggat aaacgggatt gctacaagaa acacaagagc taacaaagat ttttgttttt
     1561 taatgcacca ggcaaaaagc aatgcaagaa taaaacatac aagcccagca ataagcatat
     1621 tatatgggta aatagtaaca tgttgcaact caaacggaat attatgctgt gcaagtatat
     1681 cctgtatgta ccgggcaacc gtatccaggg tgtcgctgtg attgggcctt ggtatggaca
     1741 tgtgttttaa aatttcatac aggttcatag gaatagtatg aatcaattat ttactaatgc
     1801 aacaataata taatttttta attttaataa taaaggtgac tccatcataa cattggcgtt
     1861 gttttttagc aataattcca ttgtatgtac tgcattacca aacacggcat acatcagtgg
     1921 tgtacagcca tgattatcac gggcattgat attacatttt ttggcaagta acagtgaagc
     1981 aatctcaaca ttatctgtat atgcagcaat cataagtagc gttgtaccat tagcatccct
     2041 gaatgtacaa tctaaaacct ggtccatgag ttttttagct tcactattgt ttttggtata
     2101 gatggctgtt ggtaaattac cattgttgtt ttcttttcca tattgtgctg ccaaccagaa
     2161 acgagtttgc catctatctc tgtagggagc atattccatc tccttgtaac tattaagttt
     2221 tgcatatttt ttttcaatag caatggctat gttcataagc tcttttgaag agcactcagc
     2281 tgttaatttg ttaaatgctt cctctttaaa ataactatcg cccagtcctt ctataaatgt
     2341 ttgtataagc ttattttttg atagtgagca ataggtaagt ggtgaaggca gatcccatca
     2401 tgataacggg atgaaatcaa tataatacaa cacctgcgtg gtggagagcc cattgttttt
     2461 ttgttgggta aaagttttga taatgggttt actctgcaat cacatccaca aatgtaaaat
     2521 gaatggcatc aaccaggcct cgattactca acctgagttc aggtattaca ggaagccc
//
LOCUS       DAOSZP010000331         2573 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_31099_length_2573_cov_3.551231, whole genome shotgun sequence.
ACCESSION   DAOSZP010000331 DAOSZP010000000
VERSION     DAOSZP010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2573
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31099_length_2573_cov_3.551231"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1306)
                     /locus_tag="PK348_07580"
     CDS             complement(<1..1306)
                     /locus_tag="PK348_07580"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HXK66112.1"
                     /translation="MHINQNKNFTEDTNLTRGLGLPSAVSIVISRIIGSGIFRTPGPI
                     MALVGSTMLFGSVWIIGAVITIFAAVCYAELVAMMPRSGGPYIYLKEAYGPLIAFLRG
                     WAMFFVSETGAIAAVALVFAEYTQASLEISSGIYLSHAMVVIIALLIIVFHTFINCFG
                     VRLSGIVQIIISSSKIIALGYIITACFTKNGNVSNFYTPLLPEHFTLTHVLGIGAALR
                     YAFFAFSGWEGATYVAEEVKNPRKNLPMSLLIGIACVLILYLSANAAYLFQVPVDKFA
                     ISKWIAVDALQTALGATGALLVAYAVMINTFGNVGTQVMVKARTWQAMAQDNLFFSWC
                     AHVHTTYKTPNNAMIMQGVWACVLVLFASIYKNSYEAVIDYFSATGTIFNILTLTSVI
                     VLRRKYPEAVRPFKTWGYPVTVILVVLFYVIYLIVTLITAFMP"
     gene            complement(1312..1905)
                     /locus_tag="PK348_07585"
     CDS             complement(1312..1905)
                     /locus_tag="PK348_07585"
                     /inference="COORDINATES: protein motif:HMM:NF023251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HXK66113.1"
                     /translation="MAKDQSEKTVIDTRGYSCPISVALIAKTMKTVSDGSSVEIISDD
                     PSMKKEIEQWCVESGNIMNTVVVDHLTARFEIIKGKGYKPQSLCDLIAFYLLGVRLHI
                     RKIFVSLFRKDTTQCLITFVSVAEGMRAHYWLQNNHPELSYALLPVPNSITRHCGVVI
                     GLSSLKQARELFDLLRSNDFKVEDIYIKHNYTYHPAQ"
     gene            complement(1932..>2573)
                     /locus_tag="PK348_07590"
     CDS             complement(1932..>2573)
                     /locus_tag="PK348_07590"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic peptidoglycan transglycosylase"
                     /protein_id="HXK66114.1"
                     /translation="GALLSYYSHHNPYTTPLMAYRLYVDGYKVKPVKFIPFQKIPDDI
                     KKMVVSIEDYKFYKHNGIDIEAIKRAIVINYKVGYRMYGASTITQQLSRTLFLLPAKS
                     IIRKYVELLIALEMEMILDKDRILELYLNYCELGKGVFGIKNASYYYFGRNIYQLSTD
                     EKSRLLAILANPILYSPYDFKNSKLITNRYYILKFRYYTYNKYRSMLQYAYHD"
BASE COUNT          865 a          509 c          419 g          780 t
ORIGIN      
        1 aaggcataaa tgcagtaatc aatgtaacta tcaaatatat tacataaaat aaaactacca
       61 gaattacagt cacgggatat ccccacgtct taaatggcct tacagcctca gggtattttc
      121 tacgtaaaac aattacgctt gttagagtca atatattaaa aatggttcct gttgctgaaa
      181 aataatcaat aactgcttca taagaatttt tgtatattga tgcaaaaagt acaagtacac
      241 acgcccacac accctgcata atcattgcat tattaggggt tttatatgta gtatggacat
      301 gtgcacacca tgaaaaaaac aaattatcct gtgccatggc ttgccatgta cgcgccttta
      361 ccataacctg cgtgccaaca ttgccaaatg tatttatcat tactgcataa gcaacaagta
      421 atgcccccgt agcacccagt gcagtctgta atgcatcaac tgctatccat tttgaaattg
      481 caaatttatc taccggtacc tgaaacagat acgctgcatt ggcactcaaa tagagaatca
      541 aaacacacgc aatgccaatc agtaaagaca ttggcaaatt ttttctggga ttcttgacct
      601 cttcggcaac atacgttgcc ccttcccaac cactgaatgc aaaaaatgca taccgtaagg
      661 cagctccaat tcccagaaca tgtgtcaatg taaaatgctc tggtaaaagc ggtgtataga
      721 aattactaac attaccattt tttgtaaaac acgctgttat aatataacca agcgcaatga
      781 tttttgatga actgataata atttgtacaa tcccactgag tctaacacca aaacaattaa
      841 taaaggtatg aaacactatt ataagtaaag caatgataac aaccatagca tgtgatagat
      901 atatccctga agaaatttca agggaagcct gagtatattc ggcaaagaca agtgcaacag
      961 cagcaatagc tcctgtttca gatacaaaga acattgccca cccacgtaaa aatgcaatca
     1021 aaggaccata tgcttctttt aaatagatat aaggcccgcc agaccgtggc atcattgcta
     1081 ccagttcagc atagcaaact gcagcaaaaa ttgtaataac tgcaccaata atccatacgc
     1141 ttccaaaaag catagttgat cccacaagcg ccattatggg tccgggtgtt ctgaagattc
     1201 ccgatccaat tatgcgactt attacaatag aaacagctga aggtaacccc aatccacggg
     1261 taaggtttgt atcttcagta aaattcttat tttggttgat atgcatgatg gttattgcgc
     1321 aggatggtag gtatagttat gctttatgta tatatcttca actttaaaat cattagaacg
     1381 caatagatca aataactccc ttgcttgctt aaggctagat aatcctatta ccactccgca
     1441 atgcctggtt attgaattgg gaacaggtaa taaagcatat gataattcag gatgattgtt
     1501 ctgtaaccag taatgggcac gcattccttc ggcaaccgac acgaatgtga ttaagcattg
     1561 ggttgtatct tttctgaaaa gagaaacaaa aattttgcga atatgcaatc gtacacctaa
     1621 tagataaaaa gcaattaaat cacataatga ttgtggttta tatccttttc ctttaattat
     1681 ttcaaatcgg gcagttaaat gatcaactac tacggtattc atgatattac ccgattcaac
     1741 acaccattgc tctatctctt ttttcatgga ggggtcatct gaaataatct ctacagatga
     1801 gccatcactg acagtcttca tcgttttagc aattaatgca acagatatag gacaactata
     1861 gccacgagta tctataactg ttttttcact ttgatcttta gccataaaac tatgacctgc
     1921 tagagtatac cttaatcatg atatgcatac tgcaacatgc ttctgtattt attatacgta
     1981 tagtacctga actttaatat ataatagcgg ttggtaatca gttttgaatt tttaaaatca
     2041 tacggcgaat aaagtattgg atttgccaga attgctaaca atcgtgattt ttcatcagtg
     2101 cttagttgat atatatttct tccaaaatag taatatgatg catttttaat gccaaaaact
     2161 cctttgccta actcacaata gttaagatat aattcaagta ttctatcctt atccagaatc
     2221 atttccattt ccagtgctat caacagttca acatatttcc ttataatgga tttagctggt
     2281 agcaaaaaca gagtgcgcga tagttgctgg gttatagtgc ttgcgccata cattctgtaa
     2341 ccaactttat aattaataac tatcgcccgt ttgatagctt caatatcaat accattatgt
     2401 ttataaaact tgtaatcctc aattgagaca accatcttct tgatgtcatc aggaattttc
     2461 tgaaatggga taaattttac cggtttaacc ttgtaaccat caacatacaa ccggtatgcc
     2521 attaacggtg ttgtatatgg gttatgatga gaataatacg atagtaaagc ccc
//
LOCUS       DAOSZP010000332         2566 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_31208_length_2566_cov_3.060932, whole genome shotgun sequence.
ACCESSION   DAOSZP010000332 DAOSZP010000000
VERSION     DAOSZP010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2566
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31208_length_2566_cov_3.060932"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..585)
                     /locus_tag="PK348_07595"
     CDS             complement(<1..585)
                     /locus_tag="PK348_07595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric-oxide reductase large subunit"
                     /protein_id="HXK66115.1"
                     /translation="MLKPQWRAVLFFLLILCFGVLIFGGYMIKKEKPPIPEKVISPDG
                     VVLFTGDDIIKGQNYYFSRGGHHIGTIWGHGSYLAPDWSADYLHRAGLFVAARFTGLN
                     ADESAAFSQEYFDKLDAVKKAELTALVTKEMKENRYDAVQKVLTFTAYQAEAFEHLKK
                     YYTNLFTQGNDRMGLQPGIVTGEHEGHIVTSFFAW"
     gene            complement(698..1051)
                     /locus_tag="PK348_07600"
     CDS             complement(698..1051)
                     /locus_tag="PK348_07600"
                     /inference="COORDINATES: protein motif:HMM:NF007714.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HXK66116.1"
                     /translation="MATNNKAIEHDIGVLGKFIQIYCNGKHNGQKSKVKGNGKIAQYI
                     DPLNVELCDDCKKLLLYAASKRIICPYDPKPACKDCTTHCYAEPNKSKIREVMRYSGM
                     RMIFKGSISYIKKFL"
     gene            complement(1093..1683)
                     /locus_tag="PK348_07605"
     CDS             complement(1093..1683)
                     /locus_tag="PK348_07605"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HXK66117.1"
                     /translation="MNVQNLDENLIAILDKLTDIKRSMLWKISGHYNLTPLQIQILQY
                     INGCSATRNITPSDIVKNLYISKATASVALKTLQQKAMIRKNVDKHDSRSYYLTLTKK
                     AENILTQIEQSKRDVIQFLENMSSNDKKAAFTVLTQLVTAMQNNGIIDYVALCINCEF
                     CKNVKPHTFQCTVTGRIFEYDGINVGCCNFLDKRAV"
     gene            complement(1881..2252)
                     /locus_tag="PK348_07610"
     CDS             complement(1881..2252)
                     /locus_tag="PK348_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012387478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66118.1"
                     /translation="MDSKERIALIRKGNEFFNQGKIKEAIEIFVKTGYKDGLQRIGDY
                     YYYDKKLPLIALKFYRMSGSNKKIDEIMERMIFALGKWLGNDKVKDSPFRVKLPPLKV
                     SPKLKMLAQEILEKHKEEENK"
BASE COUNT          762 a          506 c          388 g          910 t
ORIGIN      
        1 ccatgcaaag aaacttgtaa caatatgtcc ttcatgttca ccggtaacaa taccaggctg
       61 caaacccatg cggtcattac cctgtgtaaa aagatttgta taatacttct ttaaatgctc
      121 aaatgcttct gcctgatatg cggtaaacgt aagtaccttt tgaacagcat catatcggtt
      181 ttccttcatt tcttttgtaa ccagagctgt taactcagct ttttttaccg catcaagttt
      241 atcaaaatat tcctgcgaaa acgcagctga ttcatcagca tttaacccgg taaatctggc
      301 tgccacaaaa agccctgcac gatgcagata atcagccgac cagtccggtg caagatatga
      361 gccatggccc cagatagtac caatatgatg acctccccgg ctgaaataat aattttgccc
      421 tttgatgata tcatctccgg taaataagac tacaccatca ggacttatca ccttttctgg
      481 aattggaggt ttttcttttt taatcatgta acctccaaaa attaacacac caaaacacag
      541 tatgagcaaa aaaaacagta ctgcgcgcca ctgtggtttt aacataacaa acctccttta
      601 ggtatttaat ttactgtaaa tttaaaataa aaattaatcc attgttaaaa aagcatcaca
      661 ttcacttttt gtaaaaagct ggttatcaga caacatatta caaaaatttc ttaatatatg
      721 aaatacttcc cttgaagatc atccgcattc cggaataccg catgacctca cgaattttag
      781 atttgttggg ttcggcgtag caatgggtgg tacaatcttt gcaggcaggc ttgggatcgt
      841 atgggcatat aatacgcttg cttgcagcat aaagcaataa ttttttacaa tcgtcacaca
      901 actctacatt aagtgggtca atatactgag caatttttcc attgcccttt acttttgact
      961 tctgaccatt atgcttaccg ttacaatata tctgaataaa ttttcccaat acgccaatat
     1021 catgttcaat tgctttgttg tttgtagcca tgcattactc ctgattataa aataattccc
     1081 aacacacaaa atttacactg cccgtttatc cagaaaatta cagcatccaa cattaatacc
     1141 atcatattca aaaatccgtc ctgttactgt gcactgaaat gtgtgtggtt ttacgttttt
     1201 gcaaaattca caatttatac ataaggccac gtaatcaatg atgccattat tctgcattgc
     1261 tgtaaccagt tgagttaaca ctgtgaatgc agccttttta tcattggatg acatattctc
     1321 taaaaactgt ataacatccc tttttgattg ctctatctgt gttaaaatat tttctgcctt
     1381 ttttgttaat gtgagatagt aactgcggct atcatgttta tctacatttt tgcgtatcat
     1441 ggccttttgc tgcaacgttt tcagtgcaac acttgcagta gctttactga tgtatagatt
     1501 cttcacgata tcagatggtg taatgtttct ggttgcagaa cacccgttaa tgtattgcaa
     1561 tatctgaatc tgcaatggcg taagattata atgtccagat attttccaca gcattgaacg
     1621 tttgatatca gttaatttat caagtatagc tataaggttt tcatcaaggt tttgtacatt
     1681 catatataac ccttcatata gttagtagta ctaactataa tctgcatata aaaaatgtca
     1741 aatttttttc taattttttt cagtgtttat tcataatttt ttaaggcaat acatgctgtt
     1801 ttaactttga ccgtttcagc aaggatactc attcactacc taactacaaa aagcagttat
     1861 aaaaattttc actgagtttt ttatttattt tcttcttctt tatgtttttc cagaatttcc
     1921 tgagccagca tttttaattt gggggatact tttaagggag ggagtttaac cctgaatggg
     1981 ctatctttaa ctttatcatt gcccagccat ttgccaagag caaatatcat ccgttccatt
     2041 atttcatcta tctttttatt gcttccactc atcctgtaaa atttcaatgc aatcagtgga
     2101 agctttttat cgtagtaata gtaatcgcct atgcgttgca gtccatcttt ataaccggtt
     2161 ttaacaaaaa tttcaatggc ttccttgatt ttgccctggt taaaaaattc atttcctttg
     2221 cgtattaagg ctattcgttc tttactatcc atcaatcacc ttttcacagt aactatcgca
     2281 cataactatc attacaaatt tatgtacaac acaattttgt ataacctatc tactttttat
     2341 tgacatatag tatgcttagc gatagatttg tttactagta ctttttatta ttatcgtact
     2401 gtttgcaagt attatttatg tagtttctct aacagttaac gtaaactgtt agtttggcga
     2461 tagttaccgt acaatacaat ttgactgaag agatgagccc atggcgcata tagtgattga
     2521 taccaaccgt tgtaaaggat gttacttctg tgttaagtac tgcccc
//
LOCUS       DAOSZP010000333         2566 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_31212_length_2566_cov_2.722820, whole genome shotgun sequence.
ACCESSION   DAOSZP010000333 DAOSZP010000000
VERSION     DAOSZP010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2566
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31212_length_2566_cov_2.722820"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2319)
                     /locus_tag="PK348_07615"
     CDS             complement(<1..2319)
                     /locus_tag="PK348_07615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66119.1"
                     /translation="MSKRLLWIYSILLTFFFAYTVHAASMFHPRYTWYSIKTSHFWIH
                     YHDGLEHEALRLATIAEEVHEKLKENINWQPFFRTDVVLCDTTDMANGFSMPFPYNRV
                     QLFVVMPHVEDAGLSNTDDWLRMVFTHEYTHTLTMDMIGGIPEATRYTLGRVCFPNIL
                     MPVWAVEGYPVLQESQRKPWGRLNANYTLMVLRQEVYSKNLKDISLASNFPREWPAGM
                     VPYLYGGLFVEFCTRNYPEKDFGEIFKENADNILPFLMEKNFNDVYGARAIALWQIWQ
                     KELINDLNDQIAEIKKRGISDYTVLTSTGFYTSFPRFYNNDIVFIAMTNKEKPSLVKY
                     NTHNKTFSRLAYVNMPNSLAVSNKTIIVSDVEYYQSFDLFRDVFIYDTKYSQKTKGLR
                     VQYIEPFKSGYVAILYDKGKYSLVQLDATFTIQKYFIHNTPFQISYLRVLGDSSKAVF
                     SIVHNGFSDIALYDFTTEKMLLLTNDSFVDIHPTFHPDGQKVIFSSDRDGVFNLYEIN
                     LISKKISRISNVIGGAFFPDISNNGTTIVFSSYEANGYNVALMPSATGQSQELSPIPF
                     TIQQYEEHKELLQQPYNPIYSVIGPWWAPYVYSEELYPDKYDNHIGFNTSGNDTLYRH
                     SYSLGLDYALLQKRMTLALDYIYSGLYPDLFISYKDEGIIFDDTFPWEPPDTVAYMRR
                     TLKRKVSSGISIPFQKFYNYNQLLLYYTYEKTMVSEYYSNTVFTHDIVLAGSHVVFFH
                     DGTYTGSFSVSPEDGMVFESYADIYDSSLSSDI"
BASE COUNT          841 a          489 c          440 g          796 t
ORIGIN      
        1 aatatctgaa gaaagtgaag aatcgtaaat atccgcatag ctttcaaaaa ccatgccgtc
       61 ctcaggactt accgaaaagc ttcccgtata tgttccatca tgaaaaaaca ccacatgtga
      121 gcccgcaagg acaatatcat gtgtaaaaac agtattacta taatattcac taaccattgt
      181 tttttcatag gtataataca aaagcaactg attataatta taaaatttct gaaaaggaat
      241 tgaaatgcct gacgatactt tacgtttcag ggtccttcgc atataggcaa cagtatcagg
      301 cggctcccat gggaacgtat catcaaaaat aatcccttca tctttatatg aaataaacaa
      361 atcaggatac aaacctgagt aaatataatc aagggcaagt gtcatcctct tctgtaacaa
      421 tgcataatcc agacctaacg aataactgtg cctgtacaga gtatcattac ccgacgtatt
      481 aaaaccaata tgattgtcat atttgtccgg gtataactct tcactatata catatggtgc
      541 ccaccatggt ccaatcacgg aatagattgg attatatggt tgttgtaaaa gttctttatg
      601 ttcttcatac tgctgtatgg taaaaggtat gggcgatagt tcctgtgatt ggcctgttgc
      661 agagggcatc agtgcaacgt tataaccatt ggcttcgtat gaagaaaaaa ctatagttgt
      721 gccattattg cttatatcag ggaaaaaagc gccaccaatg acattgctga tacgagaaat
      781 ttttttactt ataagattta tttcatacag attaaacaca ccatccctgt cagaagaaaa
      841 aataactttc tgcccatcag gatgaaatgt aggatgaata tcaacaaagg aatcattggt
      901 taaaaggagc atcttttctg ttgtaaaatc atacagtgct atatcactga agccattatg
      961 tacaatactg aagactgctt tggagctatc gcccaacacc cgcaaatacg aaatttgaaa
     1021 aggcgtattg tgtataaaat acttctgtat ggtaaaagta gcatcaagct gtaccagtga
     1081 atatttccct ttatcataga gtattgccac ataaccagat ttgaatggtt ctatatattg
     1141 aacgcgcaac ccttttgttt tttgtgaata ctttgtatcg taaataaaaa catccctgaa
     1201 caaatcaaat gactggtaat actctacatc actcactatg atagttttgt tgctcacagc
     1261 aagactattg ggcatgttaa cgtatgccag acgtgaaaat gttttattgt gagtattata
     1321 ttttacaagc gatggttttt ctttatttgt catggcaata aaaacaatat cattattata
     1381 aaaccgtgga aaactggtat aaaagcctgt actggttaac acagtataat cagatatgcc
     1441 acgttttttg atttctgcaa tctgatcatt caggtcattt attaattctt tctgccatat
     1501 ttgccacaaa gctatcgctc gtgctccata cacatcatta aaattctttt ccatcagaaa
     1561 tggtagaata ttatccgcat tttctttaaa tatttcacca aaatcttttt caggataatt
     1621 tcgtgtacag aactctacaa aaagaccacc atataagtag ggcaccatgc ctgcaggcca
     1681 ttcccgtggg aaattacttg ccagggatat atccttgagg ttttttgaat atacttcctg
     1741 ccgtaaaacc ataagggtgt aattagcgtt gagtctaccc cagggttttc gttgtgattc
     1801 ctgtaataca gggtatccct caacagccca tactggcatg agtatatttg gaaaacatac
     1861 tcttcccagg gtatagcgtg tagcttctgg aatgccacca atcatatcca tggtaagagt
     1921 atgtgtgtat tcatgagtaa aaaccatacg cagccagtcg tcagtattgg ataagcctgc
     1981 atcctcaaca tgtggcatta ccacaaaaag ctgcaccctg ttatacggaa acggcattga
     2041 aaatccattt gccatatccg tagtatcaca caatacaaca tctgttcgga aaaaaggctg
     2101 ccagtttata ttttctttaa gtttttcatg aacttcttca gcaatagtgg ccagtcgcaa
     2161 tgcctcatgc tctaaaccat catgataatg aatccagaaa tggctggttt taatggaata
     2221 ccaggtatac ctcggatgaa acatacttgc agcatgtacc gtatatgcaa agaaaaaagt
     2281 aagcaggata ctataaatcc ataataatcg ttttgacata tagtgacacc ttgaatttac
     2341 tattctatac gatttatcaa aatttataat ggcttgatac aattctattg tatgaatgta
     2401 cacccactgt aagtagagga gtgtatgatt tattattcat aaaatagtta tacattactg
     2461 caacaacaaa atcataatgg tttaatcatg gtagttcctg atttcgtttg aaaatttgtc
     2521 taccatttca accagtgatt ttgatgcatg ggcgactctc caaatt
//
LOCUS       DAOSZP010000334         2565 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_31228_length_2565_cov_3.010757, whole genome shotgun sequence.
ACCESSION   DAOSZP010000334 DAOSZP010000000
VERSION     DAOSZP010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2565
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31228_length_2565_cov_3.010757"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1180)
                     /locus_tag="PK348_07620"
     CDS             complement(<1..1180)
                     /locus_tag="PK348_07620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HXK66120.1"
                     /translation="MPSPTQLSNKEKYSFAVAGFGQNMVITFTVTFMLVYLYEAVGFS
                     LSGIAALTAIITGAKIWDAINDFIMGMIVDHTRTRWGKLRPYILFTSFPIALLTILLF
                     AIPQTTETYKLIYFGIVYVLWGMIYTIADVPYWGLAGSITVTIPDRVSLIAIARTLQA
                     IALAITTIASPLLAHWLSFAEKTTAAGWTRTAIIISVLGMGLFTLAFFNTEEKVPLPQ
                     QKQTIKQMIRAIVTNKPFLLVLIGSALNFGRSIVQVGGAVVAAIVFGDEKIFTLLGGA
                     LIAAIIISNLITPFVLRYMSKKTLMIASSIVAALVYTAMYYIGYSNLWIFVFMLFVSG
                     FFTGFFIVVQTAMIADCVDYYEYKTGERNEGVSFAGLTFISKLMGALATMAFGMVVVA
                     I"
     gene            1469..2311
                     /locus_tag="PK348_07625"
     CDS             1469..2311
                     /locus_tag="PK348_07625"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXK66121.1"
                     /translation="MKSNTIVVTGGNAGIGRAIATELVRQRYHVVIVSRNAAKGKEAC
                     DTIKKVTGNPRVDLIEGDLGSIQSVKSIAQKILKNFPDVSVLINNAGIWPQKLEINSD
                     GLEMAFMVNHMAPLILSIMLYKQLKKNKPSRIVNVNAGLYVFGNVDIEKTPYGKDFGK
                     LSTYMNTKLCNIYFTQKFSHIIENSGVTINAVHPGVIRTNLGDSSGIMGLLLRILKRA
                     WAEPEYGAQAPVWLAVSPDVENINGKFFDRFTQKPYAKNALDTSLREKLWDLSLQLAS
                     LTFK"
     gene            complement(2341..>2565)
                     /locus_tag="PK348_07630"
     CDS             complement(2341..>2565)
                     /locus_tag="PK348_07630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4442 domain-containing protein"
                     /protein_id="HXK66122.1"
                     /translation="ILWDKSSTIRFLKPARTSLIAKIKIPDDEFDAIQHELKHNESVE
                     RTYTIEWKDNAGNIVAQIDKVLYFKNKKAL"
BASE COUNT          845 a          463 c          511 g          746 t
ORIGIN      
        1 caatagctac tacaaccatg ccaaatgcca ttgttgcaag cgcacccata agctttgaaa
       61 taaatgtaag ccctgcaaaa cttacgcctt cattgcgctc accagtttta tattcataat
      121 aatcaacgca gtcagctatc atggctgtct gcaccacaat aaaaaacccg gtaaaaaaac
      181 ctgagacaaa cagcatgaaa acaaaaatcc acaggttgct gtagccaata tagtacattg
      241 ctgtatacac taaagctgcc acaatactgg aagctatcat caatgtcttc tttgacatat
      301 accgtaaaac aaatggggta atcaggtttg atataattat tgctgctata agtgcaccgc
      361 caagcagagt gaatattttt tcatcaccaa atactatcgc cgccaccaca gctccaccaa
      421 cctggacaat actacgtcca aaatttaatg cactgccaat caatactaaa aggaatggtt
      481 tattggtaac tatcgctcta atcatttgct ttatggtttg cttttgctgt ggaaggggta
      541 ccttttcctc tgtgttaaaa aatgctaatg taaaaagtcc cataccaagt actgatataa
      601 tgatagcagt gcgtgtccag cctgcagcag tggttttttc agcaaagctt aaccagtggg
      661 caagcagggg gcttgcaatg gtggtgattg ctaatgctat tgcctgtaat gttcgtgcaa
      721 tggcaatgag gcttacccta tcaggtatgg taaccgtaat ggaacctgcc agtccccagt
      781 agggaacgtc agcaatggtg tatatcattc cccacaatac atagacaatg ccaaaataga
      841 ttagtttata tgtttcagtt gtttgtggaa ttgcaaaaag cagaattgtt aaaagcgcaa
      901 tgggaaaaga agtgaataaa atatacggac gtaatttgcc ccaccgggtg cgggtatggt
      961 caacaatcat gcccataata aaatcgttaa tagcatccca tatcttggca ccggtaataa
     1021 ttgcagtgag tgcagcaata cctgacaggc tgaatccaac agcttcatac aggtatacca
     1081 gcatgaaggt tactgtaaac gtgataacca tattttgccc aaatccggca acagcaaaag
     1141 agtatttttc cttgtttgat agttgtgtgg gtgaaggcat gattttctcc tataaagaaa
     1201 gaataattca ggtcaaaaat taccagtacc tgtgttgtac actgtcatgc tgaacatgat
     1261 ccaggatcta tgtatcaata taattatcgc atcagtcaac atttaaatca atacacataa
     1321 tgaagaataa gaactatttc atagtttatg ggttacttta catttagtaa taaaaagtaa
     1381 attatcggca gttgttgaga taaaaataca attgacaatc aggtttttta ttactatgat
     1441 taaagaaaat cattaaaaag gatatactat gaaatcaaac acaatagtgg taaccggtgg
     1501 taatgcagga ataggcagag ctattgcaac ggaattagtc aggcaaaggt atcacgttgt
     1561 gattgtttcg cgcaatgccg ctaagggtaa agaagcatgt gacacaataa aaaaagttac
     1621 agggaatccg cgcgttgacc tgattgaagg tgatcttggt tccatacaat ctgttaaaag
     1681 tattgcacag aagatactga aaaattttcc ggatgtttcg gtgttaatca ataacgcggg
     1741 aatatggcca cagaagcttg agattaattc tgatgggctg gagatggctt ttatggtaaa
     1801 tcacatggca ccgcttatcc tgagtatcat gctgtataag caactgaaaa aaaataagcc
     1861 atcacgaata gttaatgtaa atgcagggtt gtatgttttt ggcaatgttg atatcgaaaa
     1921 aaccccttat ggaaaagatt ttggaaaact ttcaacctat atgaatacaa agttatgcaa
     1981 tatttatttt acgcagaagt tttcacacat tattgaaaac agtggcgtaa caataaatgc
     2041 agtgcatccc ggcgttatac ggacaaattt aggcgatagt tccggaataa tgggattatt
     2101 gttacggatt ttaaaacgag catgggcaga acctgagtat ggtgcacagg cgccagtatg
     2161 gcttgctgta agccctgacg ttgaaaatat caatggaaaa ttttttgaca ggtttactca
     2221 gaaaccgtat gcaaaaaacg ccctggacac ttcattgaga gagaagctgt gggatttaag
     2281 cttacagcta gcatcactga cgtttaaata aagtatatgt aacaatcttg atttggcggt
     2341 ttataatgct tttttattct taaagtatag tactttatca atttgggcaa ctatatttcc
     2401 agcgttatct ttccactcaa tggtatatgt tctttcaact gattcattgt gttttagttc
     2461 atgctgtata gcatcaaact cgtcatctgg aattttaatt ttagcaatga gtgatgttcg
     2521 tgctggctta agaaaacgaa tggttgatga tttatcccac aatat
//
LOCUS       DAOSZP010000335         2561 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_31293_length_2561_cov_2.653631, whole genome shotgun sequence.
ACCESSION   DAOSZP010000335 DAOSZP010000000
VERSION     DAOSZP010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2561
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31293_length_2561_cov_2.653631"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            35..427
                     /gene="rpsI"
                     /locus_tag="PK348_07635"
     CDS             35..427
                     /gene="rpsI"
                     /locus_tag="PK348_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012639336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HXK66123.1"
                     /translation="MASAVNYATGRRKSAVARVWLKPGNGTIIINNKPINEYFRRQTL
                     ELVVRQPLEEVGVLNSFDVVATVDGGGWTGQAGAVKLGIARCLSALDEKYKKSMRQAG
                     FLTRDSREVERKKYGQKKARKRFQFSKR"
     gene            522..1160
                     /locus_tag="PK348_07640"
     CDS             522..1160
                     /locus_tag="PK348_07640"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HXK66124.1"
                     /translation="MIIQDIEYTDRYIRITLDTGEYFRVDNDSVPSGLLRRGVAIDID
                     AYNHLKEESMLFECKEKALYYISIRSRTEHDIKKYLMKKGFDKTTISRVLQYFTERGL
                     INDYEYAVHFIQSVREHKVIGNKMLERKLFEKGVDKGIIKKAIRKTVDKEPDIEQVYQ
                     LALKKYKQVKEKHNPHVKVVAFLQQRGFGWEIIKKVMKRFGDDYEDIHDMQE"
     gene            1277..>2561
                     /gene="alaS"
                     /locus_tag="PK348_07645"
     CDS             1277..>2561
                     /gene="alaS"
                     /locus_tag="PK348_07645"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF013570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HXK66125.1"
                     /translation="MMKVHDLRTSFITFFKERDHRIVPSSSLIPKDDPTLLFTTAGMV
                     QFKPMFAGTVELEYTRAASIQKCLRTSDLENVGKTKRHCTFFEMLGNFSFGDYFKKEA
                     IQWAWEYSTDVIGFPKEDIWVSIYIDDDEAFDIWHKNIGLPKEKIVRLGKEDNFWGPA
                     GDSGACGPCSELYLDRGPSFGCGKPDCKPGCDCERFLEFWNLVFNQYFQDTEGVLHPL
                     PRTGIDTGMGLERLATLVQNVDSIYQTDELKGLVDYIVKQRNISYKGDAQIAVNVLVE
                     HARALTFAISDGAYPSNEGRGYVLRRILRRALRYGRQIGIHEPFVYTIVDPLVAAMGK
                     QYPELSDTKENVKAVIKGEEERFLETIENGMDRLEEIIKTLTRKGESTISGNDVFVLY
                     DTFGFPVEMTEEIARENGLRIDREGFEYKMEEQRNR"
BASE COUNT          832 a          391 c          612 g          726 t
ORIGIN      
        1 aaaaattatc gttactaaaa atgaggttat aaaaatggca agtgcagtaa attatgcaac
       61 aggaagaaga aaatcagcgg ttgcgcgtgt atggttaaaa ccgggaaatg gaactataat
      121 aatcaataac aaaccaataa acgagtattt caggaggcaa accctggagc ttgtggtacg
      181 acagccactg gaagaggtgg gggtactgaa ttcatttgat gttgttgcca ctgttgatgg
      241 tggtggatgg acagggcagg ctggcgctgt taaactgggt attgcacgat gcttgtcagc
      301 gctggatgaa aagtataaaa aatctatgcg gcaggcagga ttccttacaa gggattcacg
      361 tgaggttgaa cgtaaaaaat atggccagaa aaaggcacgg aaaagattcc agttttccaa
      421 acgataaaaa atggaatgca atatatactt tacaaaaagg atgccaggtg gcatcctttt
      481 tgttatgtct gcatgaacgc ctttaatatg tgtgttatgc aatgattata caggatattg
      541 aatatactga tcgctatatt cggataacac tggatactgg cgaatatttt cgagtggaca
      601 atgattctgt cccttcagga ttgttacgaa ggggtgtggc cattgatatt gacgcatata
      661 atcatttaaa agaagaatcc atgctctttg agtgtaagga aaaggcgttg tattatatca
      721 gcatccgtag ccgtactgaa cacgatataa aaaaatatct catgaaaaag gggtttgata
      781 aaactactat cagcagggta cttcaatatt ttactgaacg gggactcatt aatgattatg
      841 aatatgcagt gcactttatt caatcggtca gggaacataa ggttattggc aataaaatgt
      901 tagaaagaaa actttttgag aaaggtgttg ataaggggat cattaaaaaa gctattagaa
      961 aaacagtgga taaagagcct gatattgagc aggtgtatca gctggcatta aaaaaatata
     1021 aacaggtaaa agaaaaacac aaccctcatg taaaggtagt ggcctttttg cagcaaagag
     1081 gatttggctg ggaaattatt aaaaaagtta tgaagcgatt tggcgatgat tatgaagaca
     1141 tacatgatat gcaggaataa tttcagaagt tattttgaaa tttactataa ttaatatatt
     1201 gaaattgtaa catcgttttt ttacattgtt ttgaacatta aagtgatttt atttttattt
     1261 ataaggaatg cacagtatga tgaaggtaca tgatttgcgg acatcattta ttacattttt
     1321 taaagagcgg gatcacagaa ttgtcccttc aagttcactt ataccaaagg atgatccaac
     1381 actgctgttt acaactgccg gcatggtgca gtttaaaccc atgtttgccg gtactgttga
     1441 actggaatat acccgtgcag cgtcaataca aaaatgtttg cgaacaagtg acctggaaaa
     1501 tgttgggaag accaagcggc attgtacctt ttttgagatg ttaggaaatt tttcatttgg
     1561 tgattatttt aaaaaagaag ctattcagtg ggcgtgggaa tattcaaccg atgttattgg
     1621 ttttccaaaa gaggatatct gggtttctat atatatagat gatgatgaag cgtttgatat
     1681 atggcataaa aatattggcc tgccaaaaga aaagatagta cggttaggca aggaagacaa
     1741 tttctggggt ccagccggtg attcaggtgc atgcgggccc tgttcagaac tatatttaga
     1801 ccgtgggcct tcatttggat gcggcaagcc tgattgcaaa cccgggtgtg attgtgaacg
     1861 gtttttagaa ttctggaatt tagtgttcaa tcagtatttt caggatactg aaggtgttct
     1921 gcatccactt ccacgtaccg ggattgatac cggtatggga ctagagcgcc tggcaacgct
     1981 ggtacagaat gttgacagta tctatcaaac cgatgaattg aaaggccttg ttgattatat
     2041 tgttaaacag cggaatattt catacaaagg cgatgcacag atagctgtca atgtgcttgt
     2101 ggaacatgca cgtgcactta cttttgcaat atcagatggt gcctatccgt caaatgaagg
     2161 caggggctac gtattgcgcc gtatcttacg acgagcgctc aggtatggcc gccagattgg
     2221 tattcatgaa ccgtttgtgt ataccattgt tgacccgctg gtcgcggcca tggggaaaca
     2281 atatccggaa ctatcggaca ctaaagaaaa tgttaaagct gttataaaag gtgaagaaga
     2341 gcgcttttta gaaacaattg aaaatggcat ggaccgactg gaagagatta tcaaaacatt
     2401 aaccagaaaa ggggaatcaa caattagcgg gaatgatgtg tttgtactct atgatacatt
     2461 tggttttccg gtggaaatga ctgaagagat agcccgtgaa aatggattgc gtattgaccg
     2521 tgaaggtttt gagtataaaa tggaagaaca gcgtaaccgc g
//
LOCUS       DAOSZP010000336         2560 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_31312_length_2560_cov_2.624351, whole genome shotgun sequence.
ACCESSION   DAOSZP010000336 DAOSZP010000000
VERSION     DAOSZP010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2560)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2560)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2560
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31312_length_2560_cov_2.624351"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1526
                     /locus_tag="PK348_07650"
     CDS             <1..1526
                     /locus_tag="PK348_07650"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HXK66126.1"
                     /translation="VVEGKLNVTGVAIPGEPSIVCGHNEHIAWGMTNVMVDDMDFYLE
                     KVNPNNKMQYQFNGQWKNFTVVKEDIKIKGGETVSKEILFTHRGPVVTEFKKTNDVIS
                     MRWMGNEPSNELLTVYMLNRAKNWNDFTSAIRHFRSVSQNIVYADTSGNIGLYCAAGI
                     PIRKGNPMMVNPGWTDAYDWKGIVPFEQQPHVYNPKEGFVASANNKTVMEQYPYYISY
                     WFEMPWRYNRITKLLQQKVKLSVNDFKAIQTDQVSELAIIWKPLLLQIIEGDSSLKES
                     LKDIYTILKDWDGSYTVDSSAATLFDTFYIKLSYNIFHDELGDELYEELAKERVLVNL
                     FIDTIRVHKGSIWCDDISTPAKESFNDIIARSLKDALDLLDDDFGSNPEKWKWGKAHK
                     LVLKHPLGEVKVLDWLFGYNRGPYPIGGSFHTVCPYSYKLNNPFVITDGASQRHIFPM
                     HNLNESLTVIPTGTSGHTASPYYCDQTLLYVGMKYHNDYIDSEIIQRNAKFIMTLVP"
     gene            complement(1579..2085)
                     /gene="tpx"
                     /locus_tag="PK348_07655"
     CDS             complement(1579..2085)
                     /gene="tpx"
                     /locus_tag="PK348_07655"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HXK66127.1"
                     /translation="MPALKITANGNPVTLEGQMPSVGDKALDFTVLDINFAPVSLLNF
                     KGNIIIISAVPSLDTSVCQLQTTRFNQEAASLDAVVLTISMDLPFAQKRFCESFNIHN
                     IKTLSDFKDKSFSLSYGIYIRELGLIARSVWIIDKAGTIAYRQIVPDITQEPDYDDVL
                     KAARALGA"
     gene            complement(2101..>2560)
                     /locus_tag="PK348_07660"
     CDS             complement(2101..>2560)
                     /locus_tag="PK348_07660"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HXK66128.1"
                     /translation="LFISGCASTSIPVTKTYEDAANVLANQLKPELYNLSEHKNIKIA
                     ILPFGGPKGKPTPFGKKMATLLQSKLFSQNWNLIERDQIDKLINEKNLSATGIVEDKD
                     YMSTGSIAGADFIIVGTTYYGKKALITVKIINVHTGSIEGIAQVYLTFSK"
BASE COUNT          828 a          487 c          490 g          755 t
ORIGIN      
        1 aggtggttga aggtaaactc aatgttaccg gcgttgcaat acctggggaa ccaagcattg
       61 tatgtgggca taatgaacat atagcatggg ggatgaccaa cgttatggtt gatgatatgg
      121 atttttactt agagaaggta aaccccaata acaaaatgca ataccagttt aacgggcaat
      181 ggaaaaactt cactgtagta aaagaagaca taaaaatcaa aggtggtgaa actgtttcaa
      241 aagaaattct tttcacacac cgtggccctg tggttacgga attcaagaaa acgaatgatg
      301 tgatatccat gcgctggatg ggcaatgagc caagcaatga attactgaca gtgtatatgc
      361 tcaaccgcgc aaaaaactgg aacgatttta ccagtgccat tcgtcacttc cgctcggtaa
      421 gccagaatat tgtgtatgct gatacttcag gcaacatagg tttgtactgt gctgctggca
      481 ttcccatacg aaaaggaaat cccatgatgg tcaatcctgg atggacagac gcgtatgact
      541 ggaaaggcat tgttccgttt gagcagcagc cacatgtgta caaccccaaa gaaggatttg
      601 ttgcatcagc aaacaataaa acagtgatgg aacagtatcc ttattatatc agctactggt
      661 ttgagatgcc atggcgctat aatcgcatca ccaaactgtt acagcaaaaa gtcaaattat
      721 cagttaatga tttcaaagca attcaaaccg atcaggtatc agaactggct atcatatgga
      781 aaccattact gttacaaata atagagggcg atagttcact gaaagaatcg ttgaaagata
      841 tttacacaat actgaaagat tgggatggta gttatactgt tgacagtagt gctgccacac
      901 tatttgatac attttacatt aagctttcat ataatatctt ccatgatgag ttaggcgatg
      961 agctttacga agaacttgca aaagaaaggg ttctggtaaa tctttttatt gataccatac
     1021 gcgtacataa gggttctata tggtgtgacg atatatcaac accagcaaaa gaatctttca
     1081 atgatatcat tgccaggtca cttaaagatg cgctagattt gcttgatgat gattttggca
     1141 gtaatcctga aaaatggaaa tggggaaagg ctcacaaact ggtattaaaa cacccgctgg
     1201 gcgaagtaaa ggttttagac tggttgtttg gatataatcg cggcccatac cccattggtg
     1261 gaagtttcca taccgtgtgc ccatattcct ataaacttaa caatccattt gtaataactg
     1321 atggtgcatc acagcgacac atattcccca tgcacaatct taatgaatca ttaacggtta
     1381 ttcccactgg taccagtggc cacactgcca gcccatacta ttgcgatcaa acactgttgt
     1441 atgttggcat gaagtaccac aacgactata ttgacagtga aataatacag cgcaatgcaa
     1501 aattcataat gacgttggtg ccataaacaa acaataaaaa ggggctcttc atatagcccc
     1561 ttttatttcc aaattaattt atgcacccaa cgctctggca gctttcaata catcatcata
     1621 atctggttcc tgagtgatat caggaacaat ctgacgatat gcaatggtac ctgctttgtc
     1681 aattatccac actgaacggg caattaatcc caattctcgt atataaatgc cataacttaa
     1741 actgaaactt ttatctttaa agtcggataa tgttttaata ttatgtatat tgaaagattc
     1801 acaaaagcgt ttctgggcaa atggcaaatc catgcttatg gtcagaacca cagcatccaa
     1861 tgaagctgct tcctgattga atcttgttgt ctgcaactga cacactgagg tatcaagtga
     1921 aggcacagcg ctgattataa taatgttacc tttaaagttt agcagagaaa caggtgcaaa
     1981 attaatatca agtactgtaa aatccagagc tttatcacca actgatggca tctggccttc
     2041 aagagtcacg ggattgccat tggctgtaat tttcaatgct ggcataaaac ctccttattt
     2101 tcattttgaa aacgtaagat atacctgtgc aataccttca attgaaccag tatgtacatt
     2161 tataatcttg actgtaatta atgccttttt cccataatat gtagtaccca caataatgaa
     2221 atcagctcct gctattgatc ctgtactcat ataatcttta tcttcgacaa taccagttgc
     2281 agatagattt ttttcgttaa ttagtttatc tatttgatca cgttcaatta aattccagtt
     2341 ttgcgaaaac aactttgatt gcaataatgt tgccattttt ttaccaaaag gtgtaggttt
     2401 gcctttggga ccaccaaatg gcaatatggc gatctttata tttttgtgtt cagataaatt
     2461 atacagctcc ggctttaatt gattggctaa aacatttgca gcatcttcat atgtttttgt
     2521 aacaggtata gatgtactag cacatccaga gataaataag
//
LOCUS       DAOSZP010000337         2556 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_31373_length_2556_cov_3.761295, whole genome shotgun sequence.
ACCESSION   DAOSZP010000337 DAOSZP010000000
VERSION     DAOSZP010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2556
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31373_length_2556_cov_3.761295"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..665)
                     /locus_tag="PK348_07665"
     CDS             complement(<1..665)
                     /locus_tag="PK348_07665"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HXK66129.1"
                     /translation="MELQQALKQTLTFILSGGQGERLYPLTKDRSKPSVHFAGTYRII
                     DFSLSNCLNSDLSRIIVLTQYKSLSLDRHIKTAWNLYYRESGNFIDVVPPQQRYDENW
                     YLGTADAIFQNIYIIEHEKPHYTLILSGDHIYKMDYRKMLKYHIEKDADMTIAACEMP
                     LEEAQRFGVLVTDSSLQVTGFEEKPQNPKPVPGKPDVAYVSMGIYIFTTEKLVRYVSD
                     DHKL"
     gene            complement(666..1025)
                     /locus_tag="PK348_07670"
     CDS             complement(666..1025)
                     /locus_tag="PK348_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014809506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXK66130.1"
                     /translation="MKKILIVEDEQHQRELYVMELQEEGYEVDQAANGKEAVDKVKVN
                     KYDCVVLDIRMPEMDGIEALGKILSRDKKVPIIFYTAYSNYKSNFMTWTADAYITKSS
                     NLKELKDKIKELVSARS"
     gene            complement(1153..>2556)
                     /locus_tag="PK348_07675"
     CDS             complement(1153..>2556)
                     /locus_tag="PK348_07675"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXK66131.1"
                     /translation="IKLSYMISNNEPQIIMVMDDVTSKTKRDYQISLITRLSEKIQKD
                     DVLDHVLRTILTAVTSGTGLGFNRAMLFLKDEKNNVLYGRMAVGPDTLEEAFAIWSSL
                     SPQKDKEILSRGVNGDDAHGQLLKEKVTSTSFDLSEETIFTEAFKTGETIHVYDAYHD
                     PRVNDEIRSFIDVNEFVIVPLIALNKTIGIVIADNKFNQMPITQDSVDLLTVFAYQAA
                     LSIESYNSLDLLKKEMEKIKQRQEAIVESEKLAAIGRIASHIAHEIRNPLVTVGGYSR
                     RILQNITQNPPDVQKIKKAAQIIMTETERLEKILANVMDFTRPSPFLLKFNNINDIIR
                     DTASLLSNLFQERRVELSLQLYEVPLIKSDFNQMKQVMLNLLQNALDATPPYGVVEVK
                     TYADDRYVYIDVSDTGTGIDEENINIIFEPFYTTKTTGVGLGLAIVKKIVADHNGEIK
                     VTNRERGAVFTISIPLP"
BASE COUNT          700 a          507 c          473 g          876 t
ORIGIN      
        1 agcttatgat cgtcagaaac ataacgaact aatttttcgg tagtaaatat atagattccc
       61 atggaaacgt atgcaacgtc aggtttgcca ggaactggtt ttgggttttg aggtttttcc
      121 tcaaagccag ttacctgtaa tgagctatcg gttaccagaa ccccaaagcg ctgggcttct
      181 tcaagtggca tttcgcatgc agcaatcgtc atatctgcat ctttttcaat atgatatttc
      241 agcatcttac gataatccat tttgtagata tgatcgcccg agaggattag tgtatagtga
      301 ggcttttcat gttcaatgat gtagatattc tggaatatgg catctgctgt gcccagatac
      361 cagttttcgt cataccgctg ctgtggtggt accacatcaa taaaattacc ggattccctg
      421 taatacagat tccatgctgt ttttatgtgt ctgtcaagag acagtgattt gtactgggtg
      481 agtactataa tacggcttaa gtctgaattg agacaattgc ttaatgaaaa atcaataatg
      541 cgataggtgc ccgcaaaatg cacggaaggc tttgacctgt cttttgtcaa cgggtacaac
      601 cgttcaccct gcccacctga aaggatgaat gtaagtgttt gttttagtgc ctgctgcagt
      661 tccatttagc ttcgggctga taccagttct ttgattttgt ctttcagttc ttttaaattt
      721 gatgatttgg taatgtatgc atctgctgtc caggtcatga agttgctttt atagttggaa
      781 tatgcggtat aaaaaatgat gggcactttt ttatcacgcg aaagaatctt ccctaaggct
      841 tctatgccat ccatctcagg catgcgtata tcaagtacca cacaatcata tttatttact
      901 ttgactttat caaccgcttc cttgccattt gctgcctgat caacttcata tccttcttcc
      961 tgaagctcca ttacatacag ttctcgctga tgttgttcat cttcaacaat aagtattttc
     1021 ttcatagcaa cctcctggtg tttttctact atgtaatgta cactatttat ataaaaaatc
     1081 aaaaacattt tttatatgca aatgattaat gaactgtttg cctttatcac aaatggatct
     1141 ataaaaaata ccttatggca atggtattga aatggtaaac actgctcctc gttccctgtt
     1201 agttactttt atttcaccat tatggtcggc tacaattttc tttactatag caagtcccaa
     1261 ccccacaccg gttgtcttgg ttgtataaaa aggctcaaag atgatgttaa tgttttcctc
     1321 atctattcct gttccggtat cgctgacatc aatatagaca tatcggtcat ctgcatatgt
     1381 cttaacttca actacaccat acggtggggt tgcatcaagg gcattttgca aaaggtttag
     1441 cataacctgc ttcatctgat taaaatctga tttgattaat ggtacttcat aaagctgtag
     1501 cgatagctcc acgcgccttt cctggaaaag attggaaagc agggatgccg tgtctctgat
     1561 tatatcattg atattattga actttagcag gaacggggag ggacgtgtaa agtccattac
     1621 attggcaaga attttttcaa ggcgctctgt ttcggtcatt atgatctgtg ctgctttttt
     1681 tattttctgt acatcaggtg ggttctgggt tatattctgc aatatcctgc gtgaatatcc
     1741 accaacagtc accagggggt tgcgaatttc atgtgctatg tgtgatgcaa tacgaccaat
     1801 agctgccagt ttttcagatt caacaattgc ctcctggcgc tgctttattt tttccatttc
     1861 ttttttaaga agatcaagac tgttatagct ttctattgat aacgctgcct gatacgcaaa
     1921 taccgttaaa aggtcaacac tatcctgagt gattggcatc tgattaaatt tattgtcggc
     1981 tataacaatg cctatagttt tattcagggc aataagcgga acaattacaa attcgttaac
     2041 atcaatgaaa gatctgattt catcattgac acggggatca tggtatgcat catatacatg
     2101 aattgtttcg ccggttttaa atgcttcagt aaaaattgtt tcttcactca aatcaaatga
     2161 cgtagatgta actttttctt tcaataattg accatgggca tcatcgccat ttacaccacg
     2221 gcttaaaatt tccttgtcct tttgtgggct taaactgctc cagatggcaa atgcttcttc
     2281 aagggtatca ggaccaactg ccattctacc atagaggaca ttattctttt catctttcaa
     2341 aaaaagcatt gcacggttaa atccaagtcc tgtccctgaa gttacagcag ttaatatggt
     2401 ccgtaataca tgatccagca catcatcttt ctgaattttt tctgatagtc tggtaataag
     2461 tgaaatctgg taatcacgct tagttttact ggtaacatca tccattacca taattatctg
     2521 tggctcatta ttggaaatca tatatgacaa tttaat
//
LOCUS       DAOSZP010000338         2553 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_31417_length_2553_cov_2.790232, whole genome shotgun sequence.
ACCESSION   DAOSZP010000338 DAOSZP010000000
VERSION     DAOSZP010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2553
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31417_length_2553_cov_2.790232"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1891
                     /locus_tag="PK348_07680"
     CDS             <1..1891
                     /locus_tag="PK348_07680"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXK66132.1"
                     /translation="LVAASLVYTPLLFIERRRGIWFYYTANMLLLIIFTLYSRFHLGL
                     QRWEAIEYFMDSSLALTFVAVISYLIFSIHKLGIINAKKAEEKIQRQYEELSATNEEL
                     EAMNEELAAINEELEQTQQELLVSNINLQKETARLQAILVSIADGIVVTDENGIITSI
                     NAAALKLFGLHNDVIGEQFKEYCNIYNAKTGTPYADPVTQVLTSGKPVSLYRHTVIRN
                     NGDIYHTHIVCTPLYVANRIDGCIVDIRDMTDMVRYEEELIKTAKYESLSIFAGGIAH
                     DFNNMLSAILGNISIIEQLTPNNEKVLKASERAQKAIMKARDLTTQLLAFSKGGAPVK
                     TSTSLVNIIKDTAEFVLSGSPIKCNVEFENNLHNALVDPTQISQVIHNIILNARQAIK
                     GQGTITITAKNATLDDPGIPVKGNFIAISIHDTGEGIPPDIMPYIFDPFFTTRDGGHG
                     LGLSIAYSIVKKHDGHITVNSSKEGTLFTIYLPVAPPNETSVYETTESYCTPSKSHIL
                     IMDDDALIAESLNEMLALQGCTAVTVSNGDDAINAFADAIKQNKPFSIAILDLTIKGG
                     KGGVEIVTELQKFKTSCKFIATTGYTNEPIIANYRDYGFDAILRKPFTMNELTQILSQ
                     LTKEN"
     gene            complement(1981..>2553)
                     /locus_tag="PK348_07685"
     CDS             complement(1981..>2553)
                     /locus_tag="PK348_07685"
                     /inference="COORDINATES: protein motif:HMM:NF014626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HXK66133.1"
                     /translation="HTAPRYNISPGEDIVALVQTPGKTLIEIRWGLIPYWAKEPSSFK
                     PLINARVETVIQKPSFKRAFFNNRCIIPASGFYEWKKEGAKKIPYFITVEDFFGFAGI
                     FDSVTVNDKQLLTCAILTTAASEQMSIIHNRMPVIIRQSEFDQWINKETSPEILTKLM
                     QPFNDRLVIYPVSTKVNNPSYKEPDCIHPV"
BASE COUNT          831 a          493 c          474 g          755 t
ORIGIN      
        1 attagttgct gccagccttg tatatacccc gcttcttttt attgaacgca ggagaggaat
       61 atggttctat tatacagcta atatgctgct acttattatt tttactttgt actcacggtt
      121 tcatttaggt ttacaacgat gggaagctat tgagtatttt atggattcaa gcttagcatt
      181 gacctttgtt gcagttattt cttacttaat tttctcaatc cataaattgg gaataataaa
      241 tgcaaaaaaa gcagaagaaa aaatacaacg ccagtatgaa gaattatcag caaccaacga
      301 agaattagaa gcaatgaatg aagaattagc tgcaatcaat gaagagcttg aacaaacaca
      361 acaggaactt ctggtcagta atattaatct tcaaaaggaa actgcccggc ttcaggctat
      421 tctggtatcg attgcagacg ggatagtggt taccgatgaa aatggaatta ttaccagtat
      481 taatgctgct gcactcaaac tctttggatt acacaatgat gtgatagggg aacaattcaa
      541 agaatattgc aacatatata atgcaaaaac cggtactcct tacgctgatc cagtaacaca
      601 ggttctaact tcaggaaagc cagtttccct atatcggcat actgttatca gaaataatgg
      661 tgatatatat catacacata tcgtttgtac tccattatat gttgccaata gaattgatgg
      721 atgcattgtt gacatacgtg atatgaccga tatggttcgc tatgaagaag agctgataaa
      781 aacagcaaaa tatgaatctt tgagcatatt tgcgggtggg atagctcatg attttaataa
      841 tatgctcagt gcaattttgg ggaatatctc aattattgaa caactaactc ccaataatga
      901 aaaggtgcta aaggcttcag aacgagcaca gaaagctatc atgaaggcac gtgaccttac
      961 aacacagcta ctggcatttt caaaaggagg cgcaccagtt aaaacatcca catcgttggt
     1021 taatatcatt aaggatactg ctgaatttgt cctcagtggc tctcccatca agtgcaatgt
     1081 tgagtttgaa aataatctcc ataatgcact tgttgaccca acccagattt cacaggtaat
     1141 ccataacatc attcttaacg cccgtcaggc aattaaagga cagggcacca ttaccattac
     1201 tgctaaaaat gcaactctag atgatccagg tataccagtt aaaggcaact ttattgcaat
     1261 atctattcat gataccggcg aagggatacc gcctgatatc atgccgtata tctttgaccc
     1321 gttttttaca acacgagatg gcggacatgg attggggctt tcaattgcat attcaatagt
     1381 gaaaaagcat gatggtcaca ttaccgttaa ttcatcaaaa gaaggaacac tgtttaccat
     1441 ctatctgcca gtagccccac caaatgaaac aagcgtctat gaaaccactg aatcgtattg
     1501 cacaccttct aaatcacata tactgattat ggatgatgat gcattaatag ctgaatcact
     1561 taatgaaatg cttgcactgc aggggtgtac tgcagtcacc gtcagcaatg gtgatgatgc
     1621 catcaacgct tttgcagatg ccataaagca gaataaaccc tttagcattg ctattcttga
     1681 tcttaccatc aaaggtggta aaggcggagt tgagatagtt actgaattac aaaaatttaa
     1741 aacttcctgt aaattcattg caacaaccgg atataccaac gagccaatta tagccaatta
     1801 cagggactat ggctttgatg caatcctgcg aaaacctttt accatgaatg aactgacaca
     1861 gatactatcg cagcttacca aagaaaactg aaataaagat taaagtaaaa atttaccatc
     1921 ttaggtagca tgagtgtaac ttcatttcat gcaccattgt tacaatcttc atgataaaaa
     1981 ttaaacagga tgtatgcaat caggttcttt atatgacgga ttatttacct ttgttgatac
     2041 cggatatatc acaagcctgt cattgaaagg ttgcatgagc tttgttagta tttctggtga
     2101 tgtttcttta tttatccatt gatcaaattc agattgccga ataatgacag gcatacggtt
     2161 gtgaataata ctcatctgtt cactggcagc agtggtgagt attgcacatg tgagtagctg
     2221 cttatcatta actgttacac tatcaaaaat acctgcaaat ccaaaaaaat cctccacggt
     2281 aatgaaataa ggaatctttt tggcaccttc ttttttccat tcatagaaac cggatgcagg
     2341 tattatacat ctattgttaa aaaaagccct tttaaaactg ggcttctgta taaccgtttc
     2401 aacacgagca ttgattaatg gtttaaatga agaaggttcc tttgcccagt acggtatcag
     2461 gccccaccgt atttcaatta acgttttgcc tggtgtttgc accagcgcca caatatcttc
     2521 ccccggacta atattgtagc gcggtgctgt atg
//
LOCUS       DAOSZP010000339         2550 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_31465_length_2550_cov_2.751503, whole genome shotgun sequence.
ACCESSION   DAOSZP010000339 DAOSZP010000000
VERSION     DAOSZP010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2550
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31465_length_2550_cov_2.751503"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /locus_tag="PK348_07690"
     CDS             complement(<1..440)
                     /locus_tag="PK348_07690"
                     /inference="COORDINATES: protein motif:HMM:NF012899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HXK66134.1"
                     /translation="MPRSAYLKHVKTIVVKVGSSLITDESGISKKQVTRLVDDVVFLL
                     KKKYRIVIVSSGAISAGSAVMKKKRNLCTIPEKQALAAIGQSILMDVYRECFNKYGYE
                     VGQILLTEDDVKHRRRFLNARHTLNALLEMGVVPIVNENDSVVI"
     gene            complement(445..1428)
                     /gene="obgE"
                     /locus_tag="PK348_07695"
     CDS             complement(445..1428)
                     /gene="obgE"
                     /locus_tag="PK348_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HXK66135.1"
                     /translation="MAKFKDNIVIKVRAGKGGAGAVSFRREKYIPKGGPDGGDGGKGG
                     DVIIQATQGYYNLGHLFKDRIYKAQNGGFGMGQNRHGKDGQDLIINVPAGTQVLDAES
                     GELLADLVREGNTVCVARGGMGGKGNAFFKTATHQAPRFAQPGMPGEERSILLNLKLI
                     ADVGLVGLPNVGKSTLLKAITNAQPKIADYPFTTLIPNLGVIERGETVIKIADIPGII
                     EGAHKGLGLGLSFLQHIERVRIILFCIEAIDADPLYTLSLLKAELSTYNKDLPQRTSM
                     ILLTKSDSVNESVILKRVALLEKEKYTVLPISSLTGYNMDILIEKLFLLME"
     gene            complement(1857..2117)
                     /gene="rpmA"
                     /locus_tag="PK348_07700"
     CDS             complement(1857..2117)
                     /gene="rpmA"
                     /locus_tag="PK348_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015123382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HXK66136.1"
                     /translation="MAHKKGGGSSRNGRDSRSQRLGVKVFGGQFVKSGSIIVRQRGTK
                     LHPGTNVKRGSDDTLFAVTDGIVKFERLDKERKKVSVYPVQA"
     gene            complement(2161..2514)
                     /locus_tag="PK348_07705"
     CDS             complement(2161..2514)
                     /locus_tag="PK348_07705"
                     /inference="COORDINATES: protein motif:HMM:NF016233.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HXK66137.1"
                     /translation="MITIIVQGIDVTNNHFSLSGSVFAVRITGHAGQASKGNDIVCAG
                     VSALSQSVALALEYYEIAHSISQQEGLLEFSVNLDSLLDDKKILCESLVSVFILGINE
                     IRKQYPEFVRVSIKE"
BASE COUNT          749 a          592 c          406 g          803 t
ORIGIN      
        1 ttaattacaa ctgaatcatt ctcattaact atcggtacaa cacccatttc caaaagggcg
       61 tttaaggtat ggcgggcatt taaaaacctg cgccgatgtt ttacatcatc ttcggtcagt
      121 agtatctggc caacttcata accatatttg ttgaagcatt cacggtacac atccataagt
      181 atggattggc ctatagccgc cagagcttgc ttttcaggta tggtgcacaa attacgtttt
      241 ttcttcataa ccgctgaacc ggcactgata gcgccggaac ttacaataac aatccgatat
      301 tttttcttta ataaaaatac aacatcatca accagacggg tcacctgctt ttttgatata
      361 cctgattcat cagtaattag cgatgaccca actttaacca caattgtttt tacatgtttt
      421 aaatacgcag aacgtggcat gtaactattc cattaacaaa aaaagttttt ctattaatat
      481 atccatattg tatcctgtca atgatgatat gggaagcact gtatattttt ctttttcaag
      541 cagtgcaaca cgcttcagta ttactgattc attcacacta tcgctttttg ttaacaggat
      601 catggatgtt ctttgtggca aatccttatt atacgtggac agttcagcct ttaacagtga
      661 aagtgtgtac agtgggtcag catcaattgc ttcaatacag aacaggatga tccgtacacg
      721 ctcaatatgc tgtaaaaacg aaagccccag accaagtcct ttatgtgctc cctcaatgat
      781 tcctggtatg tctgctattt taattacagt ttcaccccgc tcaataaccc ctaaatttgg
      841 gataagtgta gtaaacggat agtcagcaat ctttggctga gcattggtga tagctttcag
      901 caatgttgat ttacctacat tgggaagccc taccaatcct acatcagcaa ttaatttaag
      961 gtttagtaaa attgaacgtt cttcaccggg cataccaggc tgtgcaaacc gtggtgcttg
     1021 atgtgttgct gttttaaaaa acgcattgcc ttttcctccc ataccacctc tggcaacaca
     1081 cactgtatta ccttcacgaa caagatcagc taaaagttct ccggattctg catccagtac
     1141 ctgtgtacct gctggcacgt ttataatcaa atcctgccca tccttgccat gacggttctg
     1201 gcccatacca aaaccaccat tctgggcttt atatatccta tccttaaaaa gatgccccag
     1261 gttataatat ccctgcgtgg cctgtattat gacatcacca cctttaccac catcaccacc
     1321 atcaggacca cccttgggga tatacttttc ccttctgaac gaaacagcac cggcaccacc
     1381 tttgccagca cgcactttaa tgactatgtt atctttaaat tttgccacgt ttttgcctgc
     1441 atcacttaca ataatagaat ataggatagc tcattatatt tgtcgtacat gaaaatagag
     1501 tatattcaca cttctcatgc tgattttatc agaaacgaga aagcttctcc agtgaatcaa
     1561 catgattttt ccttacttca ttcctcaaat gacatttctt gtttcatcat ttcaaaccat
     1621 aatcttctac ctgtcatttc gaagcccgat tgcatcggga tgggaaatct ttttactaaa
     1681 ataaacaaga tttctcgcca tttcatatgt ggaatcccac ccctttcgct ttacgaagga
     1741 gagaggccgg gcatgatgtg aacataactc tcaaaataaa aaaagcttat attgttacaa
     1801 taacaataca agctttaatt attagtatag ctataactat aataatatac tgtaccttat
     1861 gcctgaacag gatacaccga tacctttttt ctttccttat caaggcgttc aaattttact
     1921 atcccgtcag taacagcaaa tagagtatca tcgctgccac gttttacatt agtgccagga
     1981 tgcagttttg ttcctctctg gcgtactata attgagccac ttttcacaaa ctgcccacca
     2041 aaaaccttga ctcccaagcg ttgggaccgg gaatcccgtc cgtttcgcga tgaaccgcca
     2101 ccttttttat gtgccatata ttcacctcat tatttcagtt attgtcgtaa tatattatag
     2161 ttactctttt atgcttacac gtacaaattc aggatattgt ttccttatct catttattcc
     2221 aaggataaaa acgctaacta aactttcaca aagaatcttc ttatcatcaa gaaggctatc
     2281 gagattgaca gaaaattcca gtaatccctc ctgctgactg atactatgag ctatctcata
     2341 atattctaaa gctaaagcaa ctgattgcga taatgccgaa acaccagcac acactatatc
     2401 attgcctttt gatgcctgac ctgcatgccc tgttatcctg acagcaaaaa cagaacctga
     2461 caggctaaag tggttgtttg taacgtctat cccctgtact atgatagtga tcactattta
     2521 actgacatat caacaatagt aatactggta
//
LOCUS       DAOSZP010000340         2549 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_31478_length_2549_cov_2.945068, whole genome shotgun sequence.
ACCESSION   DAOSZP010000340 DAOSZP010000000
VERSION     DAOSZP010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2549
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31478_length_2549_cov_2.945068"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2046)
                     /locus_tag="PK348_07710"
     CDS             complement(<1..2046)
                     /locus_tag="PK348_07710"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HXK66138.1"
                     /translation="MDYIKLYRRFYLDMLVHGNALSGLIGAPLTVLFFIWKSEISFSN
                     LHIVIVPGFIVFIVVTLMTYYWYQFLFAPFRWFFTAKAKGASFSEKHALILKERLSSV
                     VVMFSLGLIAQWVIGLAIFSITSALFYGLSFDQLLYLWFGGILSIAISIIFGSYFIPK
                     TINRLTQEGLFDDVRDVISGKPVTAFGSLIPIITGSASIVNVLLVIMLVTFGIVLSHS
                     YMQKMFANNIDTIAKQTGHRLDSFIEYTKNDCSLIASFPDRNTINRIVTLRKIYTDCA
                     ILDNGNVILSGKGRYDGVFEKALGSLKTFEGSTSFITKPVFLGKEKRAYFATGDIING
                     KIYAALIPVDSFGKFILENAVIGKTGYIFITYNDGTGLAHPHLDITNDSLLNYEWGNK
                     LFKNQNTFISYKFKGDEKFGYSSKSSLGFLLIASIPKAEMNEYSKLLALLSLSIGLFW
                     CVIATIILYRNIRRHLEPLKVAREVLSGVAQGNLTQSLAIIKGDEVGILSAAVNRLIS
                     ELRRAIAHIIQIADELASSSTQMSQAITSFTDNVQSEASTVEEISATIEEISAGMENV
                     AKRADSQNQSLTSLINLLKNLSQTIETVADTMNASVKEVELIAERSHEGSQSLNQMNA
                     TMQVLSKSSDDMKNIVKIINDISEQINLLSLNAAIEAARAGDFGRGFAVVADEISKLA
                     "
     gene            complement(2215..2469)
                     /locus_tag="PK348_07715"
     CDS             complement(2215..2469)
                     /locus_tag="PK348_07715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66139.1"
                     /translation="MLQYEATHGGSPLTFLSKPQKPEDVAKAVVRAIESKKPEIVMPS
                     SQGFITRFIMVFPRLVVMVWPLLEKMGTKNKAIIQKTIKS"
BASE COUNT          819 a          515 c          410 g          805 t
ORIGIN      
        1 agctaatttt gaaatttcat cggcaacaac ggcaaacccg cgaccaaaat caccagcacg
       61 tgccgcctct attgctgcgt taagtgataa cagattaatt tgttcagaaa tatcgtttat
      121 aatcttaaca atatttttca tatcatcaga gctttttgaa agcacctgca tggttgcatt
      181 catctgatta agtgactgac tgccttcgtg tgaacgctca gcaatgagtt caacctcttt
      241 aaccgaagca ttcatagtgt cagcaactgt ctctattgtc tgcgatagat tctttaataa
      301 attaataagt gatgttaaac tctggttctg gctatcggca cgttttgcaa cattttccat
      361 tcctgccgat atttcttcaa tagttgctga tatctcttca actgtggatg cttcgctctg
      421 cacattatca gtaaaggatg taattgcctg cgacatctgt gttgatgaag atgctaattc
      481 atcagctatc tgaataatat gagctatcgc cctgcgcaat tcactaataa gcctatttac
      541 tgctgcagat aatatgccca cctcatcacc tttaataata gccagacttt gtgttaaatt
      601 gccctgggca accccactta acacctcacg tgctactttc aatggctcta agtgacgcct
      661 gatatttcta tacagtataa tagttgctat aacacaccag aataatccaa tactcagcga
      721 tagcagcgct aaaagttttg aatattcatt catctcagct ttgggaatgc ttgcaatcag
      781 taaaaagccc agggaacttt tgctactata cccaaacttt tcatcacctt tgaatttata
      841 cgaaataaaa gtattttgat ttttaaaaag tttgtttccc cactcataat ttaataatga
      901 atcatttgta atatccagat gtggatgtgc cagacctgta ccatcattat aggtaataaa
      961 aatatatcct gttttaccta ttactgcatt ttctaaaatg aattttccaa atgaatcaac
     1021 cgggatgaga gctgcatata ttttgccatt tatgatatca cccgttgcaa aatatgctct
     1081 cttttctttt cccagaaaaa caggttttgt aataaaactt gttgaaccct caaaggtttt
     1141 taatgaaccc agagcttttt caaaaactcc atcatatctg cctttgcctg aaaggataac
     1201 attgccgtta tccagtattg cacaatctgt atatattttt ctaagcgtaa caatgcggtt
     1261 tatggtattt cgatcaggaa atgaagcaat taagctacaa tcattttttg tatattcaat
     1321 aaaactatct aacctgtgac ctgtttgctt tgcaatagta tcaatattgt ttgcaaacat
     1381 cttctgcata taactatgtg acagcacaat gccaaatgta acaagcataa tgacaagcaa
     1441 aacattaaca atacttgctg accctgtgat gataggaata agcgaaccaa atgctgttac
     1501 tggttttccg ctgataacat ccctgacgtc atcaaatagt ccctcctgcg ttagcctgtt
     1561 aatagttttt ggtataaaat atgaaccaaa tatgattgat atagcaatag ataaaattcc
     1621 accaaaccac aggtataaaa gctgatcaaa gctaagcccg tagaataaag cgctggtaat
     1681 actgaatata gcaaggccaa taacccactg cgcaataagg ccaagcgaaa acatgaccac
     1741 cacactgctt aaccgctctt tcaatataag tgcatgtttt tcggaaaaag atgctccttt
     1801 tgcttttgct gtaaaaaacc agcgaaatgg tgcaaacaaa aattgatacc agtaatacgt
     1861 cataagtgtt accactataa acacaataaa tccgggaaca ataacaatat gtagattaga
     1921 aaagcttatt tctgatttcc agataaaaaa caaaaccgtt agcggtgcac caattaaacc
     1981 tgaaagagca ttgccatgaa caagcatatc aagataaaaa cgccgataca gtttaatata
     2041 gtccataatt gcctcgcatt tttttgaaat tactatataa tttttaaaag gtatcgagac
     2101 acctgcacgt tatgagagag cagaaaaaaa gtaaggaagc tgtaaccagt ttagcacttg
     2161 aatgataaaa ttttaaaaaa ttaagatgag tggacgtttt tttatttctg ccgattacga
     2221 ttttattgtt ttttgaatga ttgctttatt tttagttccc attttttcaa gcagtggcca
     2281 caccattact accagccgcg ggaacaccat tataaaacgt gttataaatc cctgcgatga
     2341 cggcatcaca atttcaggtt tttttgactc tattgctctg accacagcct ttgccacatc
     2401 ttctggtttt tgcggcttgc ttaaaaacgt aagcggcgaa ccaccatgtg ttgcttcata
     2461 ctgcaacatg ggcgtattca ctgaatcagg aaataccgtt gcaacctaaa ttttgctttt
     2521 ttttaattct tattgcaagt taaaaaata
//
LOCUS       DAOSZP010000341         2547 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_31509_length_2547_cov_3.207865, whole genome shotgun sequence.
ACCESSION   DAOSZP010000341 DAOSZP010000000
VERSION     DAOSZP010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2547
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31509_length_2547_cov_3.207865"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(108..>2547)
                     /locus_tag="PK348_07720"
     CDS             complement(108..>2547)
                     /locus_tag="PK348_07720"
                     /inference="COORDINATES: protein motif:HMM:NF019924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HXK66140.1"
                     /translation="IMDYEGSAEGESWKVFPDNIYVNWNQFEYAAHRSLESFYRQWYR
                     FHRINKGKGYLIWANNTQVAASIKFTEHTIWIGIFNFADSSQNVALQFDNPRLPIADD
                     TYFKVVDPVYSPITKHYSYFTGKELKASKIYSVVSYTDRIKLLKLEPVSDVAPLYSEF
                     LRDSLFRLYSISNPENFKSNFMFLETIAHSSTFEAFLTFLKNHIIAQFYPQYKNFIEI
                     GFKRILFYMFKFGFKSGNDIVQLIDDLAEHDDTNISDLGKSIKFHNRPGPIIFVSAEA
                     EPFSKSGGLANVVYELPRELVSLGEEVIVIIPKYRHGDEKAMEKMNNALKKYNVQYTG
                     KNVRFMIEHATYEAGVHYAQVDGIHYYLLDHHEFFDGLYWGYTGQEKLRRRIAFARAT
                     AELITTFGLYPLFVITNDAYTGIFNGIVRSDHVYYDNPNFKRTSFFHIIHNGGWQYFD
                     SYHRYEDGKDLFSLFNLPHWRYTDFSDPNDYNKINCMATGIRFADRVITVSPSYAKQI
                     EKACDGLEKILHNVIGISNALGVDFKNRILMRFHNSGFIDEYYPRMVDALTSSTATYT
                     KIKQRYPEILEGIRNCEKITDPLRREYVTHIRNKLCLQCKLGFEINPDIMLFSMIHRI
                     AEQKGFQLLLDASYGIFTTLGFQGVIGGAVAWGDQRGSELATGLLHLQNYYPKNVNVN
                     LGFQDISIPLLCSDVFLMPSLHEPGGISQLEALACGCLVVARATGGLRDTVIPIRMKD
                     SSVEGNGFLFSDFTPSSFYDAMQRCAEFLRTSDDTTKALARKNAMQSVYYWDKPAKEY
                     IDVMYSHKEIVRVV"
BASE COUNT          762 a          514 c          494 g          777 t
ORIGIN      
        1 taaaacattc aagttttatc attacccttc cgattaaatt accagatcat tttacactcc
       61 agggcctccc acttgtactc ttggtgtttg tggaaggctc ttttatttta tactaccctt
      121 acaatttcct tatgtgaata catgacatca atatattctt ttgccggttt atcccagtaa
      181 tagactgact gcatggcatt ttttcgtgcc agtgcttttg ttgtatcatc gctggtacgt
      241 aagaattctg cacagcgttg catagcatca tagaacgatg aaggagtaaa atcagaaaat
      301 aaaaatccat taccttcaac agatgaatct ttcatgcgta tgggaattac ggtgtcacgc
      361 agacctcctg ttgcgcgtgc aacaaccaaa catccacatg caagtgcttc aagctgtgaa
      421 atgccgccag gttcatgtaa tgatggcatt aaaaacacat cactgcacag aagcgggatg
      481 ctgatatcct gaaagcctaa gtttacgttc acattttttg gatagtagtt ttgcaaatga
      541 agtaagcctg tagctaattc actaccacgc tggtcccccc atgctactgc accgccaata
      601 accccctgaa atcccagtgt agtaaatatc ccatatgaag catccagaag aagctgaaaa
      661 cctttctgct cggctatacg gtgaatcatg gaaaacaaca ttatatctgg attaatttca
      721 aaacctaatt tacattgcaa acagagtttg ttccggatgt gggttacata ctccctgcgc
      781 agaggatcgg taatcttttc acaattgcgt atgccttcaa gtatttcagg atagcgctgt
      841 tttattttgg tatacgttgc tgtgctggat gttaatgcgt caaccattct gggataatat
      901 tcatcaataa aacctgaatt gtgaaaacgc atgagaattc ggtttttaaa atcaactccc
      961 agcgcattgc ttatacctat aacattgtgc agaatttttt caagaccatc acaggctttt
     1021 tcaatctgct ttgcatatga agggcttacc gtaatgactc tatcggcaaa tcgtatgccg
     1081 gtagccatac aatttatctt gttgtaatca ttggggtcag aaaaatcggt atagcgccag
     1141 tgcggaagat taaataaaga gaacaggtct ttgccatctt cataccggtg atatgaatca
     1201 aaatattgcc agccaccatt gtgtattata tgaaagaacg atgttctttt gaagtttggg
     1261 ttatcgtagt acacatggtc actgcggaca atgccattga aaataccggt atatgcatca
     1321 ttggtgatga caaaaagtgg gtacagacca aaagtggtga taagctccgc tgtggcacgg
     1381 gcaaatgcaa tgcggcgccg taacttttcc tgaccggtat atccccagta caaaccatca
     1441 aagaactcgt gatgatccag tagataataa tgaatgccat caacctgtgc ataatgtaca
     1501 ccagcttcat aggtagcatg ttctatcatg aagcgcacat ttttaccagt gtactgtaca
     1561 ttgtattttt ttaatgcatt gttcatcttt tccatggcct tttcatcgcc atggcgatat
     1621 ttagggatta ttacaattac ttcttcaccc agacttacaa gttcacgtgg caactcgtac
     1681 acaacgttag caagacctcc acttttggaa aatggctccg cttcggctga tacaaatata
     1741 atggggcctg gacggttatg aaacttaatg cttttaccaa ggtcggatat attggtatca
     1801 tcatgttctg caaggtcatc aattaattgc acaatgtcat tgccagattt gaatccaaac
     1861 ttaaacatgt agaagaggat tcgtttaaaa cctatctcaa tgaaattttt atactgcgga
     1921 taaaattgtg caatgatgtg attttttaaa aatgtaagaa atgcttcaaa agtggaagaa
     1981 tgtgcaatgg tttcaaggaa catgaaattt gatttaaagt tttcgggatt gctgattgaa
     2041 tataatctga aaagtgaatc gcgcaggaat tcactgtaca gtggtgcaac atcgctaacc
     2101 ggttctaatt ttaaaagctt gatcctgtca gtgtatgaaa caaccgaata aatttttgaa
     2161 gccttaagct ctttccctgt aaagtaactg taatgctttg tgataggtga atatacagga
     2221 tcaactactt taaagtatgt atcatcagca atgggcaatc gcgggttgtc aaactgcaat
     2281 gccacattct gagagctatc ggcaaaatta aaaatgccaa tccatatagt atgttcggta
     2341 aatttaatgg atgctgcaac ctgagtgtta ttagcccata tcaaatatcc cttgccttta
     2401 ttgatgcggt ggaaacggta ccattgtcgg taaaatgact ccaggctgcg gtgagcagca
     2461 tactcaaatt gattccagtt tacatagatg ttatccggaa atactttcca gctttcgcct
     2521 tcggcacttc cctcataatc cataatg
//
LOCUS       DAOSZP010000342         2547 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_31512_length_2547_cov_2.769663, whole genome shotgun sequence.
ACCESSION   DAOSZP010000342 DAOSZP010000000
VERSION     DAOSZP010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2547
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31512_length_2547_cov_2.769663"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1173
                     /locus_tag="PK348_07725"
     CDS             <1..1173
                     /locus_tag="PK348_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012174971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HXK66141.1"
                     /translation="GMGCTKFGERWDADANALMVEAFNEAIADAGIDPKEIQAAWLAT
                     CFDEVNVGKSALSAAIALRLPNIAVTRVENFCASGTEAFRAAVYAVASGAYDIVLALG
                     CEKLKDIGYGGLPEFSQAIGILNSLWFPNITAPGCFAMLAGGYCAKYGLKMEDVKRAM
                     AHISVKSHANGALNPKAHLQRPVTEEQVLQSPMIAYPLGLFDCCGVSDGAACAIVCNV
                     DVAKALKKKDFVTVKALQISVSNGEEMAFSNWDGAHVVNTEKAAMRAYQEAGIKNPRE
                     ELSLLEVHDCFSITELVTMEDLHVSERGKAVNDVMDGFFDSNGKIPCQIDGGLKCFGH
                     PIGASGLRMVYEIYLQLHGRADKRQLKDPRLGLTHNLGGFPMMNVVSVAIVGRYKQ"
     gene            1293..2171
                     /gene="dapA"
                     /locus_tag="PK348_07730"
     CDS             1293..2171
                     /gene="dapA"
                     /locus_tag="PK348_07730"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HXK66142.1"
                     /translation="MFEGVFTALVTPFKNGKIDYDSLEKIIEHQIAGGVDGVVPMGTT
                     GESPTVSYEEHEEFIICVVKMVNKKIKVIAGTGSNSTDEAIYLSKAAQDAGVDGVLLV
                     NPYYNKPPQRGLIAHFEQVAKSISIPVVLYNIPGRTGINFLPESVTELVKRVPNIVAM
                     KEASGDINQMMHLIELCGDRITLLSGDDNLLLPVLGIGGKGIISVLSNLLPADVKAVV
                     TLFKAGKFEEARKKFYALLPLCRAMFYETNPIPIKAAMEMAGFCSSEVRLPLVPLSDD
                     NRKKLKDALLAYGVKL"
     gene            2168..>2547
                     /locus_tag="PK348_07735"
     CDS             2168..>2547
                     /locus_tag="PK348_07735"
                     /inference="COORDINATES: protein motif:HMM:NF013293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HXK66143.1"
                     /translation="MRIAICGIAGRMGKAILTVVLEKGHTLSQALDAEQCPLLGQDAG
                     SLISKKLDVSIESLRSAKLTADAVIDFSSPAATEVLLQKAVEYKIPLVIGTTGFSDAQ
                     KQKIEQAAQTIPIVFSPNMSVGVNL"
BASE COUNT          744 a          440 c          626 g          737 t
ORIGIN      
        1 ggaatgggat gtaccaaatt tggtgaacgc tgggatgcag atgcaaatgc acttatggtg
       61 gaggcattta atgaagcgat agctgatgca ggcattgacc ccaaggaaat acaggcagcc
      121 tggcttgcca cctgttttga tgaagtcaat gttggtaaaa gtgcattgtc agcagcaatt
      181 gcattgcggc ttcctaacat cgctgttacc cgtgtggaaa atttttgtgc aagcggcacc
      241 gaagctttca gagctgcagt gtatgctgtt gcatctggtg cctatgatat agtactggcg
      301 ttgggatgtg aaaaacttaa agatattggt tatggcggat tgccggaatt cagccaggca
      361 attggtattt taaatagcct gtggtttcct aatataactg caccgggatg ttttgccatg
      421 cttgcaggag gatactgtgc aaagtatgga ttaaagatgg aagacgtaaa aagggcaatg
      481 gcacatatat cggttaaaag ccatgcaaat ggtgcactta atccaaaagc acatctgcag
      541 cgcccggtaa cggaagaaca ggttttacaa tcacccatga ttgcatatcc tttaggtctt
      601 tttgattgtt gtggtgtgag cgatggagca gcatgtgcca tagtatgcaa cgttgatgtg
      661 gcaaaggctt taaagaaaaa ggattttgtt acagtcaagg cactgcagat ttcagtttct
      721 aacggtgagg aaatggcatt ttctaactgg gatggtgcac atgtggtaaa taccgaaaaa
      781 gcagccatga gagcatatca ggaagctggt attaaaaatc cgcgtgaaga gttgagtctg
      841 ttggaagtac acgattgctt ctcaataacc gaacttgtaa ctatggagga tcttcatgta
      901 tctgagcggg ggaaagccgt gaacgatgtt atggatggtt tttttgacag caacggcaaa
      961 ataccatgcc agattgatgg aggtctgaaa tgttttggtc atcccattgg tgcgtcggga
     1021 ctcaggatgg tatatgaaat ctacctgcaa ttacatggga gggctgacaa acgccagttg
     1081 aaagacccac ggttaggctt aacccataat ttagggggct tccccatgat gaatgtggtt
     1141 agtgtggcga tagtgggccg ctacaaacaa taatgttgtt atgtaaaaat attcaggctt
     1201 gacaaaatat tcttgctttt actatatatg tatagtatag gcgatagtta ctatcgccta
     1261 aattttttat aaaaactaca agtgaggatt tcatgtttga aggtgttttt acagcactgg
     1321 taacgccttt taagaatggc aaaattgatt acgattctct ggaaaaaatt attgagcatc
     1381 aaattgcagg gggagttgat ggcgttgttc ctatgggaac aaccggcgaa tctccaacag
     1441 tcagttatga ggagcatgaa gagtttatca tttgtgttgt gaagatggta aataaaaaaa
     1501 taaaagtaat tgctggcaca ggttccaact caaccgatga agcaatctat ctttcaaagg
     1561 ctgctcagga tgcaggggtt gatggggtgt tacttgtcaa tccatactat aataaaccac
     1621 cacagcgcgg tcttattgcg cattttgaac aggtagcaaa atccatttca ataccggtcg
     1681 tgttatacaa tatacccgga agaaccggaa taaacttttt acccgaaagt gttacagagc
     1741 ttgtgaagcg tgtacctaac attgttgcca tgaaggaagc atcgggtgat attaatcaaa
     1801 tgatgcatct cattgaatta tgtggtgata ggattacact tctttctggt gatgataatc
     1861 tgcttttgcc tgtattaggt attggcggga aaggtatcat ttctgtactt tctaatctgt
     1921 tgcctgcaga tgtaaaggct gtggtaacgc tatttaaggc tggcaaattt gaagaagcaa
     1981 ggaagaagtt ttatgcatta ttgccactgt gtcgagcaat gttttatgaa acaaatccaa
     2041 ttcctataaa agctgccatg gaaatggctg ggttctgttc ttccgaagtg cggttgccac
     2101 tggttccact ttctgacgat aatcgaaaaa aattaaaaga tgctttgctt gcctatggag
     2161 tgaaactatg agaattgcaa tatgtggaat tgccggcagg atgggcaaag ctattctaac
     2221 tgttgtcctt gaaaaaggtc atacgctttc acaggcactt gatgcagaac aatgtccgct
     2281 tttaggccag gatgcaggta gtttgatttc aaagaaactt gatgttagca ttgagtcact
     2341 tcgtagtgca aagcttactg ctgatgcagt gatagatttt tcttcacctg ctgctactga
     2401 ggtgttgtta cagaaagcag tggaatataa aattccctta gtgataggga ccaccggatt
     2461 ttctgatgca caaaaacaaa agatagaaca agcggcacaa acaattccca ttgttttttc
     2521 ccccaatatg tcggtggggg ttaatct
//
LOCUS       DAOSZP010000343         2541 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_31616_length_2541_cov_3.132341, whole genome shotgun sequence.
ACCESSION   DAOSZP010000343 DAOSZP010000000
VERSION     DAOSZP010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2541
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31616_length_2541_cov_3.132341"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(166..582)
                     /locus_tag="PK348_07740"
     CDS             complement(166..582)
                     /locus_tag="PK348_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66144.1"
                     /translation="MKVCYPLIILLILIIFFAGSTYAKDVPYTLDDRDRIIRVEVKIN
                     EMDKRFEQIDKRFEQIDKRFDQIITFLWILTAIFITITSATLGFAFWDRKTVLKPIEE
                     KVISNEKKIDSALAAFRKLATVDKKVYAILKEFNLL"
     gene            complement(720..1868)
                     /locus_tag="PK348_07745"
     CDS             complement(720..1868)
                     /locus_tag="PK348_07745"
                     /inference="COORDINATES: protein motif:HMM:NF014818.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde 3-phosphate dehydrogenase
                     NAD-binding domain-containing protein"
                     /protein_id="HXK66145.1"
                     /translation="MAVSPDAPIGVMGIGRIGKLLVWLYAAKKQHKEIVIATGRKTGT
                     SLDDLATYFEYDSTYGTFDAFIGGFNRKNAVAVKDGHVYLNGVKLIWLNEPEFRVPGN
                     IPWNKYGVDVVFDTTGKMLDPTRNDDNSLRGHLNHAKKVILTAPFKVKDKGKQIPEDS
                     ITVVGGVNFDKYDGNKHAIISNASCTTNCCAPPIKALVDHFGDKFISYALTTVHAVTN
                     SQSTLDVLPKDGDKDTRKRRSILNNMIPTSTGAAKAVLEVIPEVQALGIGSQASSIRV
                     PVNTGSIVILDVSLLGDYDNKYIHNIFRQYSEKNPDIMYYSEKQLVSSDIIGSTYSTI
                     YDAEFTHHRTTKRGDNYYTMVDLNFWYDNEFGYVCSLSRLYDLVTGRR"
     gene            complement(2046..>2541)
                     /locus_tag="PK348_07750"
     CDS             complement(2046..>2541)
                     /locus_tag="PK348_07750"
                     /inference="COORDINATES: protein motif:HMM:NF012609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HXK66146.1"
                     /translation="KLYILQIRGMAKHMFDIEELVETPQQLQEHLLGQGLAASGGAVS
                     GRAVFDINRIEIMRMRYPKDKIILIRPETNPEDVIGLQKSDGILTSVGGMTSHAVLQM
                     RRLEKSGVSDFSIMKIVEDENIAIINREQGGKIIIREGDFITIDGNTGHVYLGFHPTR
                     KAHR"
BASE COUNT          736 a          515 c          450 g          840 t
ORIGIN      
        1 atagaccatc atgattactt tgtgcagttg gggaaaacgt ttgctgagag ggtagtaaag
       61 tttatgtggt atgaggagta ttaccggaaa aagtattcgt atgtggtgga ttgggtgtaa
      121 aaaaaataag aaaaggggtc agagcttaag aacacattaa tataattata acaaattaaa
      181 ctctttcaat attgcatata ccttcttatc aacagttgcc aattttctga atgcagcaag
      241 cgcactgtca attttctttt catttgagat aactttttct tctataggtt ttaaaacagt
      301 tttcctgtcc cagaaagcaa atcccagggt tgctgaagtt attgtaataa aaatcgctgt
      361 caatatccat aaaaaagtaa taatctggtc aaatcgttta tctatctgtt caaagcgttt
      421 atctatctgc tcaaagcgtt tgtccatttc atttattttt acttccactc gtattatacg
      481 atctctatca tctaatgtat aagggacatc ctttgcatat gtagaacctg cgaaaaaaat
      541 aattaatatt aacagtatta ttagtggata acatactttc ataaatctat tccttatttt
      601 ataaatataa ttatattata ttatgctgtc aattatattt ttttaactgc agcaatcata
      661 aaaaagggct gactgatttt agccgccctt tttaatagaa taaggttaat ttacagtaac
      721 tatcgcctgc ccgtaacaag gtcataaagg cgtgatagtg aacatacata cccaaattca
      781 ttgtcatacc agaaattgag gtcaaccatc gtgtagtaat tatcgcctcg tttggttgta
      841 cggtgatgag taaattcagc atcatagata gtggaatagg tactgcctat gatatctgaa
      901 ctgacaagct gcttttcaga gtaatacata atatcaggat tcttttctga atattgcctg
      961 aatatgttat ggatgtattt attgtcataa tcacctaaca gtgaaacatc cagtataaca
     1021 atagaaccag tattcaccgg aacacgtatt gaagacgcct gagaaccaat acccaaagcc
     1081 tgtacttccg ggatgacttc cagcactgct ttagcagcac cggtgcttgt tggtatcatg
     1141 ttgtttaata tgctgcggcg tttgcgggtg tccttatcgc catcttttgg cagcacatca
     1201 agcgttgact gtgaattggt taccgcatgg acagtagtca gcgcatatga gataaacttg
     1261 tcgccaaaat ggtcaaccag ggcttttatt ggtggtgcgc aacaattggt agtacacgat
     1321 gcattggaaa ttatggcatg tttgttgcca tcatatttgt caaagttaac tccgccaact
     1381 acggttattg aatcctcagg aatttgtttg cctttatctt ttactttaaa cggtgcagta
     1441 agtataacct tctttgcgtg gtttagatgc cccctgagcg aattgtcatc attgcgcgtt
     1501 gggtcaagca tcttaccggt ggtgtcaaaa accacatcaa cgccgtattt attccagggt
     1561 atattgcccg gcacacgaaa ttcaggctca ttcaaccata tcagcttcac accattgagg
     1621 tatacatgcc cgtccttaac tgcaaccgca ttttttctgt taaatccacc aataaacgca
     1681 tcaaaggtgc cataggttga atcatattca aaataggttg ctaaatcatc taacgatgta
     1741 cccgtttttc tgccggttgc aatgacaatc tctttatgct gcttttttgc agcatacagc
     1801 cacaccaaaa gttttccaat tcggccaatg cccattacac caataggtgc atctggtgat
     1861 actgccataa taatcctcca tatgcttgtt ttgtgttatt atatcaagca ggacctaatc
     1921 ccatacccag tgtttttata tataaattaa agtaattact gataaaacta atgtaaaatt
     1981 tacacccatt acgtccattg aaaaagttag ttctaatttt tcaagctttt ttatatacgg
     2041 tacgtttacc ggtgggcttt acgggtagga tgaaacccta aatacacatg gccggtatta
     2101 ccatctattg ttataaaatc accttcacgg ataattattt tgccaccctg ttcacggttt
     2161 ataattgcta tattttcatc ttctactatt ttcattatgg aaaaatcgct gacacctgat
     2221 ttttcaagtc tgcgcatctg taacaccgca tgcgatgtca tccctcctac agatgtcaat
     2281 attccatctg atttctgtag tcctatcaca tcttccgggt tggtctctgg gcgtattaaa
     2341 attatcttat cctttggata gcgcatgcgc attatctcta tgcggtttat atcaaacact
     2401 gctcttcctg atactgcccc gcctgaagct gccaacccct gccctaacag atgctcctga
     2461 agctgttggg gtgtttcaac cagttcctct atatcaaaca tatgtttggc cataccacgg
     2521 atttgcagta tatacagctt t
//
LOCUS       DAOSZP010000344         2533 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_31735_length_2533_cov_3.588378, whole genome shotgun sequence.
ACCESSION   DAOSZP010000344 DAOSZP010000000
VERSION     DAOSZP010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2533
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31735_length_2533_cov_3.588378"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..656)
                     /locus_tag="PK348_07755"
     CDS             complement(<1..656)
                     /locus_tag="PK348_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015902858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HXK66147.1"
                     /translation="MKVDRGYQKSFLIDIDGVIVRGSSLISGAKDFIDKLKKGNYPFL
                     FLTNNSYYTPKELSERFFALGLDVDEDRFYTSAMATASFLVYQKPGCSAYVIGGKGLI
                     QELERNGIVITYKNPDYVIIGETEEYDYARIIEATYLIQEGARFIATNPDLTGPTQRG
                     PVPACGALVAPIERVTGVRPYFLGKPNPAMMYWARKKLNVHSANCFMIGDRMDTDILG
                     G"
     gene            complement(660..1499)
                     /locus_tag="PK348_07760"
     CDS             complement(660..1499)
                     /locus_tag="PK348_07760"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HXK66148.1"
                     /translation="MELIDLHCHTTASDGIYSPTGIIDYAIVHNVSVLAITDHDSIDG
                     LEEALDYAKQKLFELIPGIEFSIDFPHGSFHLVGMGIDYTNGDLRYELEHLQMLRKER
                     IYKIIKDLQLHGIEVTDEEVEKEALGNSPGRPHVARVLVKKGYAPTVSDVFTQFMVKG
                     KPGFVKKEKINFHRALQLIKASGGTAIVAHPVSLGFSSWAEGEQLIKDFVDAGVAGIE
                     VYSAMHSDEDVTHLLHIAQKYSLLISGGSDFHGDKNEAMGYYGEGKPIPAGLAKQFLK
                     TVK"
     gene            complement(1581..2336)
                     /locus_tag="PK348_07765"
     CDS             complement(1581..2336)
                     /locus_tag="PK348_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HXK66149.1"
                     /translation="MELEQYHIGCSGWNYQHWRGIFYPEEIPKSKWLQHYATVFNSVE
                     INATFYRKFKDSAYVNWYNKVPQSFCFSVKLSKYVTHIKRLVDVTDEVNSCIASASLL
                     KQKLGVILIQLPPSLKFDTSLVADFLSILPGNYRYTIEARHNSWICDSSFSLLQHYNV
                     AWCISDTAGRYPYYEEVTADFVYIRLHGSTQLYASNYSDNELHNWAEKILKWNTLTYC
                     YFDNDFNAHAVYNALSLKKLLMLASPSTCSDYM"
BASE COUNT          808 a          532 c          384 g          809 t
ORIGIN      
        1 ccacctaaaa tatcagtatc cattctatcg ccaatcataa aacagtttgc tgagtgaaca
       61 tttaactttt tacgtgccca gtacatcatc gcaggattgg gtttacccaa aaaataaggc
      121 cttacaccag taactcgttc aattggtgct accagcgcac cacacgcagg tactggacca
      181 cgctgtgttg ggccagttaa atcgggattg gtagcaataa aacgtgctcc ttcctgtata
      241 agatatgtgg cttctataat gcgagcataa tcatattctt cagtttcacc aatgattaca
      301 taatcagggt ttttatatgt tataacaatg ccattccttt ccagttcctg aatcaacccc
      361 ttccctccaa tgacgtatgc agaacaacca ggcttttggt acacaagaaa acttgccgta
      421 gccatagctg aagtgtaaaa tctatcctca tcaacatcca gtcctaaagc aaaaaacctt
      481 tcagaaagct ctttaggcgt ataatacgaa ttatttgtta aaaataaaaa ggggtaatta
      541 cctttcttga gcttatcaat aaaatctttt gcgccagaaa taagtgaact tcctcttact
      601 atcacaccat cgatatcaat taaaaatgat ttttgatatc ccctatcaac tttcataatc
      661 tattttaccg tttttaaaaa ttgctttgct aaaccagctg gtataggctt accttcacca
      721 tagtatccca tggcttcatt tttatctcca tggaaatcgc ttccacccga aataagcaaa
      781 gaatattttt gtgcaatatg taaaagatgt gttacatctt catcactatg cattgccgaa
      841 tacacttcaa tccctgccac accggcatca acaaaatctt ttattaattg ttcaccctca
      901 gcccatgaag aaaacccaag tgatacagga tgcgcaacaa ttgcagttcc accacttgct
      961 ttaatcagct gtaaagcgcg gtgaaaattt attttttctt tcttaacaaa tccgggtttt
     1021 ccttttacca taaactgagt aaaaacatca ctcactgttg gtgcatatcc ttttttcacc
     1081 agcacacgtg caacatgtgg ccttccaggg gaattaccca gagcttcttt ttcaacctct
     1141 tcatcagtaa cttcaatacc atgtagctgc aaatctttga taattttgta tatcctttct
     1201 ttccgcaaca tttgcagatg ctctaattca taccgtaaat cgccattggt ataatcaatg
     1261 cccataccga ccagatgaaa tgaaccatga ggaaaatcta tactgaactc tatgccaggt
     1321 ataagctcaa ataacttttg ttttgcataa tcaagagctt cttctaaccc atcaatagaa
     1381 tcgtggtcag taatggcaag aacgctgaca ttatgcacaa tggcataatc aatgattcct
     1441 gttggcgaat agattccatc ggatgctgtt gtgtgacaat gtaaatcaat tagctccata
     1501 gcaattaaac ctgcttaaga attttattat aatagcacaa tccctgaaaa aatacaacct
     1561 gataattttt aaaacatttt ttacatataa tcactacacg ttgaaggact ggcaagcatt
     1621 aataattttt ttaacgatag agcattataa actgcatgtg cattgaaatc attatcaaaa
     1681 tagcaatatg ttaatgtatt ccactttaat atcttttctg cccaattgtg caactcatta
     1741 tcagaatagt ttgatgcata caattgtgtt gaaccatgca atcgtatata cacaaaatca
     1801 gcagtgactt cttcataata tggatatctt cccgcagtat ctgatataca ccatgccaca
     1861 ttatagtgct gcaataatga aaatgagcta tcgcatatcc atgaattatg ccgtgcttca
     1921 attgtgtagc gatagttacc gggcagtatt gatagaaaat cagcaaccag cgatgtatca
     1981 aacttaagtg atggtggcaa ttgaataagg ataacaccta acttttgttt taataatgat
     2041 gcagaagcaa tacacgagtt tacttcatcg gtaacatcaa caagccgttt gatatgcgtt
     2101 acatattttg acaatttcac actgaagcaa aagctctgtg gaaccttatt ataccagttt
     2161 acatacgcag aatctttaaa tttcctgtag aatgttgcat taatctccac actattaaag
     2221 actgtagcat aatgctgaag ccactttgat tttgggattt cttctggata aaaaattccg
     2281 cgccagtgct gataattcca ccctgaacag cctatatgat attgttccag ttccatgtat
     2341 taatttatta tacaattaca caatgcttta agatatcact atttaattac atcaatagca
     2401 aggatgatgc tgaatcttat gtatatattg caaaatacta tgcttgatgt atttattcaa
     2461 gtatttttgc atataggctc tgaccccttt ttttacaccc actcaagtat catggagtac
     2521 ttctttcggt aat
//
LOCUS       DAOSZP010000345         2506 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_32146_length_2506_cov_3.246430, whole genome shotgun sequence.
ACCESSION   DAOSZP010000345 DAOSZP010000000
VERSION     DAOSZP010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2506
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32146_length_2506_cov_3.246430"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1676
                     /locus_tag="PK348_07770"
     CDS             <1..1676
                     /locus_tag="PK348_07770"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003869986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA topoisomerase subunit B"
                     /protein_id="HXK66150.1"
                     /translation="GIPLDIHPVYKTSALEIVLTKLHSGGKFDNQAYKVSGGLHGVGI
                     SVVNALSEYMEVEVYRDGRRYFQSYKRGVPTDKVKDIGETKKTGTRVLFKPDSQIFEE
                     TEFHFETLSKRMRELAFLNNGIKITLTDLRGKGKEHTFYYKGGIVSFVQSLTENRTSI
                     TKKVIYLHDNRSNIDVEIALDYIDTYTETVFCYANNIHTKEGGTHLIGFKSALTRVLN
                     DFLKREGYDKKIPQLSGDDVREGLVAIISVKIPNPQFEGQTKMKLGNSEVKGIVESIT
                     NEHLTQFFEENPQIVRKILEKCIDTARARIAAKKARDLTRRKNALENDSLPGKLADCS
                     ERDPALCELYLVEGDSAGGSAKQGRDRKFQAILPLKGKILNVEKSRLDKILSNDEIKT
                     IITALGTGIGENEFDITKCRYHKIIIMTDADVDGSHIRTLLLTFFFRYMPEIIANGYL
                     YIAQPPLYNIKAGKEQYYAYSDEERDAIVKNLDKSKVTIQRYKGLGEMNPEQLWETTM
                     DPEKRTLLQVTLEDEVEADEVFSVLMGDAVEPRRKFIEENAHMVENLDL"
     gene            1775..2506
                     /locus_tag="PK348_07775"
                     /pseudo
     CDS             1775..2506
                     /locus_tag="PK348_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017861868.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
BASE COUNT          841 a          409 c          557 g          699 t
ORIGIN      
        1 gtggtattcc gcttgatata catcccgtat acaagacgtc agccctggag atagttctta
       61 ccaaattaca ttcaggtggt aaatttgaca atcaggcata taaggtgtcc ggtggtttac
      121 atggtgttgg aatatctgtt gtgaatgcac tgtctgaata tatggaagta gaagtgtaca
      181 gggatggtag gcgttatttt caaagctata agcgaggggt gccaacagat aaagttaagg
      241 atattggcga aacaaagaaa acgggaacac gggtactttt taaacctgat agccagatat
      301 ttgaagaaac agagtttcat tttgaaactc tttcaaagag gatgagggag ctggcatttc
      361 ttaacaatgg cattaaaatt acattaactg atttgcgtgg aaaaggaaaa gaacatacct
      421 tttactataa aggtgggatt gtttcttttg ttcaaagctt gactgaaaac agaacctcta
      481 taacaaaaaa ggttatttac ctgcacgaca atcggagtaa tatagatgtt gaaatagccc
      541 tggattatat tgatacatat actgagacag tattttgcta tgcaaataac attcacacaa
      601 aagaaggtgg aacccatctc attggattta aatctgcatt aacccgcgta ttaaatgatt
      661 ttttaaaacg ggaaggatat gataaaaaaa ttccccagct atcaggtgat gatgtcagag
      721 aaggtttagt agctattata agcgttaaga tacccaaccc tcagtttgag gggcagacaa
      781 aaatgaaact gggaaacagt gaagttaaag gaattgttga atctataacc aatgagcatc
      841 ttacccagtt ttttgaagaa aacccccaga ttgtacgcaa aattttagaa aaatgtatcg
      901 atactgcgcg tgcgcgcata gcagcaaaga aagcacgtga tttaacccgt agaaaaaatg
      961 ctttagaaaa tgattcccta ccggggaaac ttgctgactg cagtgaacgt gatccggcac
     1021 tatgtgagct gtatcttgtt gagggtgatt ctgcaggtgg ttcagcaaaa cagggaagag
     1081 atagaaaatt tcaggcaatt ttgcccttaa aaggaaagat acttaatgtt gaaaagtccc
     1141 gtctggacaa gattctttcc aatgatgaaa tcaagaccat tattactgca ctgggtaccg
     1201 gaatcggtga aaatgaattt gatatcacaa agtgcaggta ccacaaaatt attatcatga
     1261 cagatgctga cgttgatggt tctcatatac gaacattgct attaacgttc tttttcaggt
     1321 atatgcctga gattattgcc aatggatatt tatatattgc ccaaccccca ctgtataata
     1381 taaaagctgg taaagaacag tattatgcat acagtgatga agaaagagat gcgatagtta
     1441 agaatttaga taagtccaaa gtgacgattc agcggtacaa aggtttaggg gagatgaatc
     1501 ctgaacagct atgggaaaca accatggatc cagaaaaaag aacattattg caggtcacat
     1561 tggaagatga ggtagaagca gatgaggtct tttctgtttt aatgggcgat gctgttgagc
     1621 cacggagaaa atttatagaa gagaatgccc atatggttga gaatttagat ttataaaaat
     1681 attttcctat aactatataa ttttattctt tgaataaagg taacagactg tgacaaaaaa
     1741 acaggataat cctaagaaca aacgagttca taatattgaa attgaagaat caatgaaacg
     1801 ctcatatctg gattatgcaa tgagcgttat tgtaggaaga gcacttcctg atgtcaggga
     1861 tggattgaag cctgttcatc gtagaatact tcatgctatg aatgagcggg catggcgcag
     1921 tgacaggcct tatgttaagt cagccaagat agttggtgaa gtaattggta actatcaccc
     1981 ccatggtgat gcggctgtgt atgataccct ggttagaatg gcacaggatt tttctatgcg
     2041 gttaccttta attgatggac agggtaactt tggttcaatt gatggcgacc cacctgctgc
     2101 atatcgttat actgaggcac gactatctcc cattgcacag gaactgctta aagatattga
     2161 taaagatact gtagatttta tctcaaactt tgatgaaaca cgcaaggaac cggtagtatt
     2221 gcctgcagcg ttcccaaacc tgctggtaaa tggttcctca ggtatagctg ttgggatggc
     2281 cacaaacatc ccaccccata atttatcaga agttattgat gctattgtat tcatgattga
     2341 taaccctgat gctaccgtta agcaattgat gaaatttgta aaaggccctg atttcccaac
     2401 aggtggtatt attgttgctt cagaagagat gataaaagca tatgcaaaag gcaaaggaag
     2461 tattgtggtc agagccaggc ttcatgttga ggaacagaaa aacaaa
//
LOCUS       DAOSZP010000346         2484 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_32502_length_2484_cov_3.139975, whole genome shotgun sequence.
ACCESSION   DAOSZP010000346 DAOSZP010000000
VERSION     DAOSZP010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32502_length_2484_cov_3.139975"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..717)
                     /locus_tag="PK348_07780"
     CDS             complement(<1..717)
                     /locus_tag="PK348_07780"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF010621.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HXK66151.1"
                     /translation="MKKVKENITGVVTVLLLVLNTLFWASFLFPIAVLKIIPVKIWRK
                     FINNILDTIACCWIYCNNAGLKMTRTIHWDVSGVENLSKKSWYIVIANHQSWTDIVVL
                     QKILTGKIPFLKFFLKKELIWVPVLGLAWWALDFPFMRRYSQSFLLKYPHLKGKDIEV
                     TKKACQKFKYIPVSIMNFVEGTRFTPEKHAKQESPYKNLLKPKSGGIGFVFSTMGNMI
                     TSILDVTIVYPQGRMSFWDFL"
     gene            complement(910..>2484)
                     /locus_tag="PK348_07785"
     CDS             complement(910..>2484)
                     /locus_tag="PK348_07785"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HXK66152.1"
                     /translation="TYTGPFFKDSKKRKPWPQFVIANQENAASPPKGKRGIVQSDMVF
                     VLGDSNQQNAYVFGQLPVHRQFHQFVSFEYNYRTKLLSIIWDVDRFCDAEEDYTLDTV
                     CILQGTVWKTMEYYAETIAQELSPTFNNTLRKGWCSWYYYYNTITRDEILLNAKYAKE
                     NSIPFDFIQIDDGYQATIGEWTTVRGSFTAAMRYIADTIKGYGFTPGIWYSPFIVSKD
                     SRIFKENKSWILKDNNGKPVIAGFNPAWGGYYYTVDVTQPAALSWIEDTINTLLYEWG
                     YEYLKLDFMYAAALPGVRHNTTISRYEALAQAVARIRKVSGNAFVLGCGMPLQAGIRY
                     VDAMRIGPDVAPQWGRTFFDKLFNSDSGISTRSAICSSVYRSFMHNRFWVNDPDCLMI
                     RQHKTTLKPDERQTLYNVITALGGMFVISDRLPDYSATEREMLLQAIAIFDMAKDGNI
                     YCNDVFGALHSFYNSNGLGVLLNVNDIPCEATLKQEIINNYSKILLIENNTKIPSQTK
                     KFKLLPHSSKLFLFEK"
BASE COUNT          776 a          503 c          411 g          794 t
ORIGIN      
        1 taaaaaatcc cagaatgaca ttcttccctg cgggtataca atggtaacat ccagaatgga
       61 cgtaatcatg ttgcccatag ttgagaatac aaacccaata ccccctgatt ttggttttaa
      121 gagattttta tatggtgatt cctgcttggc atgtttttcc ggtgtaaatc gtgtcccttc
      181 tacaaaattc ataatggaca cgggaatata tttaaatttc tggcatgctt ttttggtaac
      241 ttcaatatct ttccctttta gatgaggata tttcagtaaa aatgattggg aatatcgtcg
      301 cataaatgga aaatccagtg cccaccacgc taaccctaaa actggtaccc atattaattc
      361 tttttttaaa aagaacttta aaaatggtat tttgccagtc aaaatttttt gcagtacaac
      421 aatatctgtc catgactgat gattggcaat aactatatac cagcttttct tgctgagatt
      481 ttctacgcca gaaacatccc agtgtatggt acgcgtcatt tttaaaccag cgttattaca
      541 gtatatccag caacatgcaa tagtatccag tatgttattt ataaacttac gccatatttt
      601 tacaggtatt atttttaaaa ctgcaattgg aaataaaaat gaagcccaga ataatgtatt
      661 gagtaccagt aaaagtacag taacaactcc agtgatgttt tctttaactt ttttcattaa
      721 tcttctcctg aaaatttaat ttcttcatcg ttattctgaa cctgacttgg aatctattac
      781 tctattacgt ttactatcaa tcctgcaccg gtgcaataat ggataaatag gattttttat
      841 ttactgactg atttatacaa accaacaacc cattaaaaag acaccggtaa tcagtaaacg
      901 gttgcaaaat tatttttcaa aaagaaataa ttttgaactg tgaggtaata gtttaaattt
      961 ttttgtttgg gagggtattt ttgtattatt ttcaatcaat aatattttag aataattatt
     1021 tattatctcc tgtttcagtg ttgcttcaca gggaatatca tttacattta ataatacacc
     1081 caatccattt gaattataga atgaatgtaa tgccccaaaa acatcattac aataaatatt
     1141 tccatccttt gccatatcaa atatcgcaat tgcctgtaat agcatttcgc gctcagttgc
     1201 agaataatca ggcaacctat cggaaattac aaacataccc cccagtgctg ttattacgtt
     1261 atacaacgtt tggcgttcgt caggcttaag cgttgtttta tgctgacgta tcataagaca
     1321 atcaggatcg ttaacccaga agcggttatg cataaatgaa cgatacacac tgctacaaat
     1381 agctgagcgt gttgaaattc ctgaatcact gttaaatagc ttatcaaaaa atgttctccc
     1441 ccattgaggc gcaacatcag gaccaatacg cattgcatct acatacctaa tgcccgcctg
     1501 caaaggcata ccacatccca gtacaaacgc attaccgctt acttttcgta tgcgggcaac
     1561 cgcctgtgca agagcttcat accgtgatat tgtcgtgttg tgtcgcactc ccggtagtgc
     1621 tgcagcatac ataaaatcta acttgaggta ttcatacccc cattcataca ataacgtatt
     1681 aatagtatcc tcaatccagc tcagcgctgc aggctgagta acgtcaacgg tatagtaata
     1741 gccaccccat gcaggattga aaccagcaat aacaggtttc ccattgttat ctttaagaat
     1801 ccaggattta ttctctttaa aaatccttga atcttttgaa acaataaatg gagaatacca
     1861 gattccaggc gtaaatccat atcctttgat agtatcagca atatagcgca tggcagcggt
     1921 aaaagatccc ctgacagtag tccactcgcc aatagtagcc tgatacccat catcaatctg
     1981 gataaaatca aatggtatgg aattctcttt tgcatattta gcattgagaa gtatttcatc
     2041 tctggtaatg gtattatagt aataatacca ggaacaccag cccttgcgta gtgtgttatt
     2101 aaatgtaggc gatagttcct gtgcaatagt ttctgcataa tattccatcg ttttccacac
     2161 tgtaccctgc aaaatacata ctgtatccag cgtgtagtct tcctcagcat cacagaatct
     2221 atcaacatcc catataatgg acaacagttt tgtgcgatag ttatactcaa aggaaacaaa
     2281 ctgatgaaat tgtctgtgta cgggaagctg tccaaaaaca taagcgtttt gctggttact
     2341 atcgcccaat acaaacacca tatccgattg gactatacca cgctttcctt ttggtggact
     2401 ggcggcattt tcctgatttg cgatgacaaa ttgtggccag ggtttgcgct tttttgaatc
     2461 tttaaaaaat ggacctgtat atgt
//
LOCUS       DAOSZP010000347         2446 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_33109_length_2446_cov_6.026767, whole genome shotgun sequence.
ACCESSION   DAOSZP010000347 DAOSZP010000000
VERSION     DAOSZP010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33109_length_2446_cov_6.026767"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(360..1478)
                     /locus_tag="PK348_07790"
     CDS             complement(360..1478)
                     /locus_tag="PK348_07790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66153.1"
                     /translation="MKKLIVVLVMAGVVIAQPLFAATITGDCPILTIPAYSITLGVIG
                     DDLYDQFINALHDANAELAKYDDQDKLATGFANANMYSTNAATQQGYPNYSLFAITTG
                     VMVGAQAPTTNVDYYTNGKIEDDIENKGDLYAGVAGSAAVNVGINAGMIIPGLYINVK
                     FGKFDSTWVYDDDDFSFDTMIIGGGITYSLFDEAGIGLLRWRGLTIGTGLVYQTTDVS
                     YRIELDDERIHFTEGSTEGDVVVDPSFDITLDMYTLSVPFEINTAIQLLWLFNINLGV
                     GIDLVSGNADLELQSVSDATVKNIAYGGIPLPEDEYIVTPGKVTIDGKTLDKKPSWAR
                     ARIMTGIGFNFGAVKIDVPIIYYLKSGAAVGVTAGIIW"
     gene            complement(1774..1920)
                     /locus_tag="PK348_07795"
     CDS             complement(1774..1920)
                     /locus_tag="PK348_07795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66154.1"
                     /translation="MILERKYDKYDIEEVQKRIRYYPATAVSGQRQSGKTLTVQRYTG
                     CEGI"
     gene            complement(2062..2349)
                     /locus_tag="PK348_07800"
     CDS             complement(2062..2349)
                     /locus_tag="PK348_07800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66155.1"
                     /translation="MIDITLIIILVIIATVLLWTVILFKIVMSPFAINNKWKLLGVLL
                     IFALCYSMLPSWAVKTFNFKSYTIPCIIVSVLFVGLVILYTMINARLKKYK"
BASE COUNT          746 a          561 c          375 g          764 t
ORIGIN      
        1 tgctgtcaat tgccaatctg cttgtgccac cattctttct ttttatgttt tttgtgctgt
       61 ttggataatc attagcatat cctcccattt gtttaatgtt taactttgct tattggtaag
      121 atacgcgtac tttccttttt ttgtaacaat aaggttagtt tgacggatac tctaaacccc
      181 ttctctcgta ttccactacc ataacgctgt tttaccccaa cagcagcatc cgttatacaa
      241 acaaaaagaa tttgctaaaa aacttttccc ttgtatttca ccatcatatc gcttttttac
      301 cccaagagcc gcaccattct aaaataaaag gggttttgct aaaaacccct ttccccattt
      361 taccatatta tccccgctgt caccccaaca gccgcaccgc tttttaaata atagataatg
      421 ggcacatcaa tcttaaccgc tccaaaattg aagccaatcc ctgtcataat acgcgcccgt
      481 gcccatgatg gctttttatc cagggttttc ccatcaatgg taaccttgcc gggagtaacg
      541 atatactcat cctcaggtaa cggaatacca ccatacgcaa tgtttttaac cgttgcatca
      601 gacactgatt gcaactccag atcggcatta ccgctcacca gatcaatgcc aacacccaga
      661 ttgatgttaa aaagccacaa cagttgtatg gcagtattga tctcaaaggg aacgcttaat
      721 gtatacatat caagcgtaat gtcaaatgaa gggtcaacaa cgacatcgcc ttctgttgaa
      781 ccctctgtaa aatgaattcg ttcatcatct aattctattc tatatgaaac atcggttgtt
      841 tgatatacca atcctgtacc aatggttaaa ccacgccagc gcaataagcc aatgcctgct
      901 tcatcaaaca ggctataggt aatgccgcca ccaataatca tggtatcaaa gctaaaatcg
      961 tcatcatcat atacccaggt actgtcaaac ttaccaaatt ttacattgat ataaagcccc
     1021 gggataatca tccctgcgtt tatccctaca ttaacggctg cactccctgc aacaccagca
     1081 tataaatccc ctttgttttc tatatcatcc tcaatcttgc cattggtata ataatcaaca
     1141 ttggtagtag gtgcctgagc gccaaccata acgccggtgg ttatggcaaa caggctatag
     1201 ttgggatagc cctgctgcgt ggcggcattg gttgaataca tgttggcatt ggcaaaacca
     1261 gtagccagct tatcctgatc atcatacttt gccaattcag cattagcatc atgcaaagca
     1321 ttaataaatt gatcatataa atcatcacca atgacgccta atgtaattga ataagcagga
     1381 attgtcaaaa ttgggcaatc cccagtaatg gttgctgcaa atagtggctg tgcaataacc
     1441 acacctgcca taaccagcac tacaataagc tttttcataa gtcctcctat aaaaagtcaa
     1501 aatattttac tacccaattc aaataatgta aaaatataga gaataatcag ggtatgctaa
     1561 tcattttccc tcttgcatat acccggtatt ctcatggagc aaaacaactg aaaacaagat
     1621 tctttgcttt gactatgctc agtacaggca caattgcttt agaataacta tcgcccatga
     1681 caaataccat tcattacttc atctttcatt atagttactt caacatacct gctatgcttt
     1741 acaaagtgca aaaattttta tatcattttt taatcatata ccctcacatc cggtatatcg
     1801 ttgaacggtt agtgttttac ctgattgccg ttgaccactg acggctgttg cagggtagta
     1861 gcgtatgcgt ttttgtactt cttcaatatc atatttatca tattttcttt ctaaaatcat
     1921 cgttcacttg aaatttcttc gtgagctgct gatttatcat ggtatcctac cggggtattg
     1981 gatacatgtc aaccaataat cacactatcc gcgttttgta atgattaaaa ccatggtacc
     2041 tataaaccat ctatcgttaa cctatttgta tttttttaac cgtgcattga tcattgtgta
     2101 cagtatcaca agcccaacaa aaagaacgct cacaatgata cagggtatgg tatagctttt
     2161 gaaattaaat gtctttactg cccatgaagg cagcatgcta taacatagcg caaaaatgag
     2221 cagcacaccc aatagcttcc atttgttgtt gattgcaaac gggctcataa caattttaaa
     2281 taatatcacc gtccacaaaa gaactgtggc aataataacc agtattatta ttaaggttat
     2341 atcaatcata gcaactacct ttatgcgtta ttcttgtgat attcattaaa accatgattt
     2401 tttaaataca tcacaattgc gtcacggtat ttcataaaat cttctc
//
LOCUS       DAOSZP010000348         2446 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_33121_length_2446_cov_3.064826, whole genome shotgun sequence.
ACCESSION   DAOSZP010000348 DAOSZP010000000
VERSION     DAOSZP010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33121_length_2446_cov_3.064826"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..711
                     /locus_tag="PK348_07805"
     CDS             <1..711
                     /locus_tag="PK348_07805"
                     /inference="COORDINATES: protein motif:HMM:NF012928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HXK66156.1"
                     /translation="ALKFGIAQGKTCIVVKDGPGFYTTRILAPYLNEAVFLIEEGVPM
                     NDIDRALLQFGYPIGPVALLDEVGIDVGAHVAIELGQEFKARGAAPSNVLPLLFSKGF
                     KGKKNKKGFYDYSKPKKKGMRVPNEEIYKVIGKVTWQHLPMQEIQERVSLMMINEAAL
                     CLQEGIISSPRDGDVGSILGLGFPPFRGGIFRHIDREGVKTIVDTMMKYYDKGMKRFT
                     PAQILVDMAKSGKRFYKE"
     gene            715..1860
                     /locus_tag="PK348_07810"
     CDS             715..1860
                     /locus_tag="PK348_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXK66157.1"
                     /translation="MKRSIQFSQEHDIFRQTVRKFFETEVMPHYDKWEEEGIVPRQMW
                     KRSGELGLLLPWLDEKYGGPGGDFLYSVIVSEELAYSGANSFMIPLHNDIVAPYIYSF
                     GTEEQKMKYLPGCASGDIILAVAMTEPEAGSDLAAMKTSATLDGDSWVLNGQKTFISN
                     GILADLVVVAAKSSKEAPPHQAVSLFLVENGTPGFTKGKAIKKIGLKAQDTAELFFDN
                     CRIPKENLLGQEGLGFIYLMQKLQQERLVCALASQAAMEKCLAITIDYVKERKLFGKP
                     LSKFQNTQFAIAEMASEIAVGRSFIDDLILHHIRGEDVVKETCMAKYWICETAKRVAD
                     RCLQFFGGYGYCTEYPVSRFYVDARIQTIYAGTSEVMKMIISKMMGL"
     gene            1956..>2446
                     /locus_tag="PK348_07815"
     CDS             1956..>2446
                     /locus_tag="PK348_07815"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HXK66158.1"
                     /translation="MNPVIRKGYTYMAWLFIGVDTAFWGTISLFSWFVSPRGNLAHFC
                     MQNWSKNILWFCGIRYQVEGLENITKDKVQIFASNHQSFFDVWLLSAIIPVKFGWTAK
                     KELFKIPFMGWHMTLSGYIPIDRKNRERAIESMKLAAIKIRKGNRIMIFPEGTRSRTG
                     VLQ"
BASE COUNT          733 a          433 c          607 g          673 t
ORIGIN      
        1 gctctaaaat ttggcattgc gcagggcaaa acatgcatag tggtcaagga tggcccggga
       61 ttttacacaa cgcgaatact tgcaccatat ctcaatgaag cggtgttcct gattgaagaa
      121 ggtgtgccaa tgaatgatat agaccgagcc ctgttgcagt ttggctatcc aattggtcct
      181 gttgcgctcc ttgatgaggt tggtattgat gttggcgcac atgtggcaat tgagctgggg
      241 caggaattca aggcacgggg tgcagcaccg tccaatgtat tgccactttt attcagtaag
      301 ggatttaaag gtaaaaagaa taagaaaggg ttctatgatt acagcaaacc aaagaagaaa
      361 ggtatgcgtg tacccaacga agaaatctac aaggttatag gtaaagtaac ctggcagcat
      421 ttgccaatgc aggaaattca ggagcgtgta agcctcatga tgattaatga ggcagccctg
      481 tgtctgcagg agggtattat atcttcgccg cgtgacggtg atgttggttc cattttagga
      541 ttgggttttc caccattcag aggaggaatt ttccgtcata tagaccggga aggtgtcaaa
      601 accatcgttg atacaatgat gaaatattat gataaaggga tgaaacgttt taccccggca
      661 cagatacttg ttgatatggc aaagagtggc aaacgctttt ataaggagta aacaatgaag
      721 cgttctatac aattttctca ggaacatgat atattcaggc aaacagtacg caaatttttt
      781 gaaacagaag ttatgccgca ttacgacaaa tgggaagaag aaggtatagt gccacgacag
      841 atgtggaagc ggagtggaga attggggtta ctacttccct ggctggatga gaagtatgga
      901 gggcctggtg gtgatttttt gtattcggtg atagtgagtg aggaattagc ctatagtggg
      961 gctaattcat tcatgatacc gttacataat gatattgtgg caccatatat ctattcattt
     1021 ggcacagaag aacagaagat gaagtacctg ccagggtgtg cgtctggcga tataattctg
     1081 gctgtagcaa tgaccgagcc tgaggctggt tcggatttag cggcaatgaa aacgagtgca
     1141 accctggatg gtgatagctg ggtactcaat ggtcagaaga catttatatc caatggtata
     1201 ttagctgacc ttgtagttgt agcagcaaaa tcgtcaaaag aagcaccacc gcatcaggct
     1261 gtaagccttt ttctggtgga aaacggtaca cctgggttta caaagggaaa agcaataaaa
     1321 aagataggct taaaagccca ggacactgca gagctatttt ttgataactg tcgcatacca
     1381 aaagaaaatc ttcttggtca ggaagggttg ggatttattt accttatgca gaaattacag
     1441 caagaacggt tggtatgtgc tttggcgtca caggcagcaa tggaaaaatg tttagcaata
     1501 actatcgact atgtaaaaga acgcaaactt tttgggaaac cattgtcaaa attccagaat
     1561 acccagtttg ccatagcaga gatggcttct gaaattgctg ttggccgcag ttttattgat
     1621 gacctgatac tccatcacat tcggggtgaa gatgttgtca aagaaacctg tatggcaaag
     1681 tactggatat gcgaaacagc aaagcgtgtt gccgatcgat gcctgcaatt ttttggcggt
     1741 tatgggtact gtaccgaata tcccgtgtca cgcttctatg ttgacgcacg gattcagaca
     1801 atctatgctg gcacatccga agtgatgaag atgattatct cgaaaatgat gggattgtaa
     1861 tttaatagtt gacattcata gcaaagtata gtgtaatcct tgcgtttttt aaaatttgtt
     1921 caaaatttaa aatttgttga attgtggagg ccattatgaa tccagtgata cgcaagggtt
     1981 acacatatat ggcatggctt ttcattggtg ttgataccgc tttctgggga actatctccc
     2041 tgttttcctg gtttgtaagc cccaggggta acctggcaca cttttgcatg caaaactggt
     2101 caaaaaatat actctggttt tgcggcattc gctatcaggt tgaaggtctt gaaaatatta
     2161 cgaaagataa agttcagata tttgcatcca accatcagag tttttttgat gtgtggctac
     2221 tgagtgccat tattccggta aagtttggct ggactgctaa aaaggagctt tttaaaatcc
     2281 catttatggg gtggcacatg acattaagcg gttacatccc tattgacaga aaaaacagag
     2341 agcgggcaat cgaaagcatg aaattagctg ccattaaaat acgaaaaggt aaccgcatca
     2401 tgatattccc tgaaggcaca cgcagccgca cgggagtatt acagca
//
LOCUS       DAOSZP010000349         2445 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_33137_length_2445_cov_3.824268, whole genome shotgun sequence.
ACCESSION   DAOSZP010000349 DAOSZP010000000
VERSION     DAOSZP010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2445
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33137_length_2445_cov_3.824268"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="PK348_07820"
     CDS             complement(<1..219)
                     /locus_tag="PK348_07820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66159.1"
                     /translation="MLSKAILIALLCFVPLQSQFASSVCTEATALAKAKEYSKAKELF
                     IKAIGLNPYYYVAHYGYGKACLYSGDINN"
     gene            complement(209..652)
                     /locus_tag="PK348_07825"
     CDS             complement(209..652)
                     /locus_tag="PK348_07825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66160.1"
                     /translation="MNTTMLSTSLMIFTTIWGLCVLLLWMRPRIELIWKIAASGIYGF
                     YVWFFYKDLVKGYKQFEKDWYLVTIAFIKEFSALIFANMFFLWPLALIIIFYKADDIG
                     AERLLKFMCIFTLVVWLVFTLYTFYSKQIDTFLFHTLREMIPNAQ"
     gene            complement(659..1648)
                     /gene="pdxA"
                     /locus_tag="PK348_07830"
     CDS             complement(659..1648)
                     /gene="pdxA"
                     /locus_tag="PK348_07830"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:TIGR00557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HXK66161.1"
                     /translation="MQIRKPRVAITIGDPAGIGPEVTLKALCDFETLPCLPLVICRVG
                     VLQQYYHHLMPSWDILNFNEIQRDTVIQKPYFINIHSDVPIPQPSCGTELTGKESLAY
                     IDAAITLWKLKMIDAIVTGPVNKSLIEKWVPFMGHTEYIAEQIHEDNPRMLMYSGKYN
                     VMLVTTHVPVFKLKDVIDYAVLMETIQICESSLTRIMKKKPKIAICGLDPHCGDNGAI
                     GNFDKDVTAKVVKDAQAQGIIIEGPFSADTIFIPQRWEQYDCIIAHYHDQGLIPFKML
                     AFDEGVNVTVGLSIIRTSVDHGTAFDIAGKDCADYSSMKKAIELASFLALKEE"
     gene            complement(1638..2060)
                     /locus_tag="PK348_07835"
     CDS             complement(1638..2060)
                     /locus_tag="PK348_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010417822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HXK66162.1"
                     /translation="MALAKSGGDVANNVIGENSYFSGKFLINGSLRIDGRFEGKYLQA
                     DQLYIGPNGKVKTNINAVSVIIEGLVIGNINATNRVLLMPTAKILGDIKTPELIIQNG
                     VILEGRCTIANDLKISAKDLIENEYEKNKLIPEEFLAD"
     gene            complement(2201..>2445)
                     /locus_tag="PK348_07840"
     CDS             complement(2201..>2445)
                     /locus_tag="PK348_07840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66163.1"
                     /translation="LFLMDLFSFFKDVNDFVTKILADTEFGGTMVLNADDKITYARYD
                     MYKTFEGLTVKEALRYLKGFVQEVLQIIKVPDKTKF"
BASE COUNT          782 a          486 c          372 g          805 t
ORIGIN      
        1 gttattaata tctcctgaat acaggcacgc tttcccatat ccataatggg caacatagta
       61 ataaggattt aaaccaatag ctttgataaa caattctttt gcttttgaat attctttagc
      121 ttttgcaagt gcagttgctt cagtgcatac tgaggaagca aactggcttt gaagaggaac
      181 aaaacataat aacgcaatga gtattgcttt actgagcatt ggggatcatt tcgcgaagtg
      241 tatgaaataa aaatgtgtca atttgtttac tgtaaaatgt atacaatgtg aaaacaagcc
      301 atacaacaag ggtaaatata cacataaatt tcagtaaccg ctctgcacca atgtcatctg
      361 ccttgtaaaa gataattatt aacgctaatg gccataagaa aaacatattt gcaaaaatca
      421 gggcactgaa ttcttttata aaagcgatag ttaccagata ccagtctttt tcaaattgtt
      481 tataaccttt tacaagatct ttataaaaaa accacacata aaacccatat ataccagaag
      541 cagcaatttt ccatattagt tcaattctgg gacgcatcca taatagcagc acacacagcc
      601 cccagattgt tgtaaatatc attaacgaag tggaaagcat tgttgtattc attgggtatt
      661 attcctcttt taacgctaag aaactagcca attctatggc tttcttcatg ctgctatagt
      721 cagcacaatc tttgccagca atatcaaatg cagtgccatg atcaactgaa gtccgtataa
      781 tggataatcc cacagtcaca tttacacctt catcaaacgc cagcatcttg aaaggtatta
      841 agccctggtc atggtaatga gctataatac aatcatattg ttcccagcgc tgtggtataa
      901 atatagtatc ggcagaaaat ggcccttcaa ttatgattcc ttgtgcttgt gcatccttta
      961 caacttttgc agtgacatcc ttatcaaaat tgcctattgc accattatcc ccacaatgtg
     1021 gatcaagccc acatatagca atctttggct tttttttcat tattctggtt aagctgcttt
     1081 cacaaatctg aatagtttcc atcaatacag cataatcaat aacatctttt agtttgaaaa
     1141 caggcacatg tgttgtaaca agcataacat tatattttcc tgaatacatg agcattcgtg
     1201 ggttatcttc atgtatctgt tctgcaatat attcagtatg tcccataaat ggtacccact
     1261 tctctataag ggatttattg acaggaccag taacaattgc atcaatcatc tttagcttcc
     1321 ataacgtaat ggctgcatca atatatgcta agctttcctt gcctgttagt tcagtaccac
     1381 aggaaggttg tgggattggt acatcactat ggatatttat aaaataaggt ttctgtataa
     1441 cagtatcacg ctgtatttca ttaaaattca gtatatccca cgagggcatg agatggtgat
     1501 agtattgttg caatacgcca accctgcata tcaccagagg aaggcacggc agtgtttcaa
     1561 aatcgcataa tgcttttaat gttacttccg gtccaatgcc agctggatcg ccaatagtaa
     1621 tagctactct gggcttccta atctgcaaga aactcctcgg gtattaattt atttttttca
     1681 tattcatttt caatcaagtc tttggcggaa atcttaagat catttgctat agtgcagcgc
     1741 ccttctaata ttacaccatt ctggattatc agttcaggtg tttttatatc ccctaatatt
     1801 tttgctgtgg gcattaacaa taccctgttg gtagcattaa tattacctat aaccagacct
     1861 tcaataataa ctgatacagc attaatattg gttttaactt ttccgtttgg gccaatatat
     1921 agctggtcag cctgcaaata tttcccttca aaacgaccat ctattcgcaa tgaaccgtta
     1981 ataagaaatt tgccactgaa ataagaattt tcaccaataa cattatttgc aacatcacca
     2041 ccagattttg ctaaagccat aaaaacacct gcccttcatt ttttttcata gctacataat
     2101 gaatatattt atgtcaacat gaaaattgta tttacattca tgatttgtat acaccgccgc
     2161 ctgtgcggta tgcatatata cagttatgaa attttcaata ctaaaacttt gttttatcag
     2221 gtactttaat tatctgtaat acttcctgaa caaacccttt gagatatcgc aatgcttctt
     2281 ttactgtcaa accttcaaat gttttataca tatcatacct ggcgtatgtt attttgtcat
     2341 ctgcatttaa aaccattgtg ccaccaaatt ccgtatctgc aagtattttg gttacaaaat
     2401 cattaacatc tttaaaaaaa gaaaataaat ccattaaaaa taacc
//
LOCUS       DAOSZP010000350         2431 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_33366_length_2431_cov_2.545034, whole genome shotgun sequence.
ACCESSION   DAOSZP010000350 DAOSZP010000000
VERSION     DAOSZP010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2431
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33366_length_2431_cov_2.545034"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..868)
                     /locus_tag="PK348_07845"
     CDS             complement(<1..868)
                     /locus_tag="PK348_07845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66164.1"
                     /translation="MSDSTLQKLEQSYPNRIQNHALFNDAWYLFVNDYPPTHCLLDFK
                     ELPSTRVALHAGDLGFALLWIVQSVLIRCIIYNINQLQPWDIQLHNTTIGAMAHTEPD
                     TNRMIARYSDSNTLVISGKKNYISGGSDADILLLTARRPGEDKFTKMMYIPVKSLPEN
                     ALQDINLSILPTIRHSSLTLTDFACNASQCCPVKDKELRKILKKWSLIERSLIGEAYI
                     AFLCYLAKNIPLPDQDQTLNEAEELLSLQKNFSREQLENAFSDKYVDTLGDVARVLNI
                     TQKIANYCMENTN"
     gene            complement(870..2075)
                     /locus_tag="PK348_07850"
     CDS             complement(870..2075)
                     /locus_tag="PK348_07850"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008516783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HXK66165.1"
                     /translation="MSVKKVVLAYSGGLDTSVIVKWLLETYKCEVICYAADVGQEEEL
                     DGLEQKAINTGASKCYIEDLKEEFVRDFIFPCIKANAIYENRYLLGTSIARPVIAKRQ
                     VEIALKEGADAVCHGATGKGNDQVRFELTFKALAPQLKIIAPWREWNLTSRSSLFDYA
                     EKHNIPVPVTKDKPYSMDRNILHISYEGGILEDPWREPDESMFILTKSPEKAPDIPAY
                     IEIDFEQGVPVAIDGKRMDPVSLMKTLNKIAGENGVGRIDIVENRLVGIKSRGVYETP
                     AGTVLHVAHRDLEAITLDRDTQHLKDRLAIEYAELVYYGQWFTKRREALDAFINETQK
                     NVTGTVRVKLFKGTCMAVGRKSPVTLYESDIATFDESALYDHRDATGFINLFGLQTKI
                     EALLKQKRK"
     gene            2231..2431
                     /locus_tag="PK348_07855"
                     /pseudo
     CDS             2231..2431
                     /locus_tag="PK348_07855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
BASE COUNT          724 a          480 c          440 g          787 t
ORIGIN      
        1 aatttgtatt ttccatacaa taatttgcaa tcttctgtgt aatattgaga acacgagcca
       61 catctcctaa tgtgtcaaca tacttatccg agaacgcatt ttccagttgt tcacgggaaa
      121 aattcttttg taacgacaac agttcttctg cctcgttaag cgtttgatcc tgatcaggta
      181 aagggatatt cttagcaaga tagcataaaa atgcaatata tgcttcacca ataagtgaac
      241 gttcaataag gctccacttc tttaatattt tacgcagttc tttgtctttt actggacagc
      301 attgcgaagc attgcaggca aaatcagtaa gagttaacga agaatgcctg atagttggca
      361 atattgaaag attgatatcc tgtaaagcat tttcaggaag gcttttaact ggtatataca
      421 tcatcttagt aaatttatcc tcaccaggac gtctggcagt gagcaatagt atatctgcat
      481 cggaaccacc agaaatatag ttctttttac ctgaaatcac aagtgtattt gaatctgaat
      541 atcgggcaat catacggtta gtatccggtt cggtatgtgc cattgcgcct attgtagtat
      601 tatgtaattg aatgtcccat ggctgtagct ggtttatatt gtagatgata catcgtataa
      661 gaacagattg cacaatccag agcaatgcaa atcccaaatc acctgcgtgc agtgctacgc
      721 gtgttgaagg caattcttta aaatcaagta gacagtgtgt tggtggatag tcattgacaa
      781 ataaatacca tgcatcgtta aacaatgcat ggttctgtat acggtttgga tagctctgtt
      841 ccagtttttg taaggtacta tcgctcatat tattttcttt tttgctttaa taatgcttca
      901 atttttgttt gcaagccaaa caggttaata aatccagttg catccctgtg atcatacaat
      961 gcactttcat caaatgtggc aatatctgac tcatataagg taaccggtga ttttcgtcct
     1021 acggccatgc atgtaccttt aaacaacttt acgcggacag taccggttac attcttttgg
     1081 gtttcattaa taaatgcatc taacgcttca cgacgttttg taaaccactg gccataatat
     1141 acaagttctg catattcaat tgcaagcctg tcttttaaat gctgggtatc cctgtccaac
     1201 gtaatagctt caagatcgcg gtgtgccaca tgtaacactg tacctgcggg ggtttcatat
     1261 acaccccggg atttaatgcc aacaagcctg ttttctacta tatcaattct gccaacacca
     1321 ttttcgccag caattttgtt gagggttttc attaatgata caggatccat gcgtttgcca
     1381 tctattgcaa ccggcacacc ctgttcaaaa tcaatttcaa tatatgctgg tatatcaggt
     1441 gccttttctg gtgattttgt taatataaac atgctttcat caggttcacg ccagggatct
     1501 tccagtattc caccctcata tgaaatgtga agaatattgc ggtccatact gtatggctta
     1561 tcttttgtta caggaacggg tatattgtgt ttttcagcat agtcaaaaag agatgatcgt
     1621 gaagttaaat tccattcgcg ccatggtgca ataatcttca attgtggtgc aagtgcctta
     1681 aacgttaatt caaagcgaac ctgatcatta cctttgcctg ttgcaccgtg gcacacagca
     1741 tcagcacctt ctttcagagc tatttcaact tgccgttttg caataacagg gcgagctatg
     1801 gaagttccaa gtaagtatct attttcataa attgcatttg ccttgatgca tgggaagata
     1861 aaatcacgta caaattcttc tttaagatct tcaatataac attttgaagc accggtattg
     1921 atagcctttt gttcaagacc atcaagttct tcctcctgac caacatcagc agcataacaa
     1981 attacttcgc atttataggt ttcaagtaac catttcacaa taacggaagt atcgaggcca
     2041 cctgaatagg ccaatacaac ttttttaacc gacatagttg tttttctcca ttacaccaaa
     2101 tttcagtact tttgctgtca agaatattga tactgaatga cttgtcaatt gagtttaggg
     2161 ttataatatg caattttttt aatatgcaat ctttcaaaat ataaggaaat aagtatatac
     2221 atacggccat gcaacgggag atgaggtgtt aaaaaccgtt gcacaaagca tttcaacatc
     2281 attacgttct ttagatatat ttgcacggta tggtggggaa gaatttatta tatgccttcc
     2341 tgaaaccagt atcattcaag cgacaagggt agctgaacgc atcagatcat gtatcaatga
     2401 tcttacattc cacgataaaa aaaacaatca a
//
LOCUS       DAOSZP010000351         2430 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_33379_length_2430_cov_2.605895, whole genome shotgun sequence.
ACCESSION   DAOSZP010000351 DAOSZP010000000
VERSION     DAOSZP010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2430
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33379_length_2430_cov_2.605895"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(65..229)
                     /locus_tag="PK348_07860"
     CDS             complement(65..229)
                     /locus_tag="PK348_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HXK66166.1"
                     /translation="MDKKYECTVCGYIYDPKEGDPDNGIKPGTAFKDLPDDWVCPVCG
                     AGKDQFEPVD"
     gene            complement(343..537)
                     /locus_tag="PK348_07865"
     CDS             complement(343..537)
                     /locus_tag="PK348_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006465371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HXK66167.1"
                     /translation="MQFRVNDMMCNHCVMHITKAINEKDPAAKVQCNVTTKMVMVDSS
                     LDASIVMQAIKEAGYTPELL"
     gene            complement(802..1206)
                     /locus_tag="PK348_07870"
     CDS             complement(802..1206)
                     /locus_tag="PK348_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HXK66168.1"
                     /translation="MEQYKHYLEAKGISPSYQRLKILEYLDQNRTHPTVDEIYTALLP
                     HIPTLSKTTVYNTLSLFVQEGLASMLVLDNVEARFDITVQPHGHFTCTQCGRVYDLNI
                     TRYVNMPESIDGHTIEQYQITLKGICKECSSH"
     gene            complement(1288..2421)
                     /locus_tag="PK348_07875"
     CDS             complement(1288..2421)
                     /locus_tag="PK348_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015326478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HXK66169.1"
                     /translation="MYWVGGIDWDLRNFHGYMTQRGSTYNAYVIMDEKITLIDTVKYY
                     LYDEMIERISKVVDPAKIDIIISNHVEMDHSGGLPKLLELCPNATLITSPNGAKGLEA
                     HYKKNWNYKVMKTGDSISLGKTTLQFILEQMVHWPDNMACYMPETKILFSNDAFGQHI
                     ATSERFDSEYPLDIIMYEAKKYYANIVLPYDKQTQKVLDAVSTLDIAMIAPSHGIIWK
                     DHVRAIVTEYNNWAANKTKEKALIIYDTMWNSTKLIAHAIKKAFEEKNIPAQLYDLKT
                     IHISDIMTEVLDAKYICVGSPTLNNNMLPTVAAFLTYCKGLAPKNRVGLAFGSYGWGG
                     QSVAQVEAVLAEMGWQLLPSIKVQYVPDEPLLGTIAQQVFEKL"
BASE COUNT          721 a          473 c          437 g          799 t
ORIGIN      
        1 caaattaatt atttgtacaa taaaaaggat atgcagcggg tgcatatcct ttaattaatt
       61 ggtatcaatc aacaggttca aactgatcct ttccagcacc acacacgggg caaacccagt
      121 catcaggtaa atctttaaaa gctgttccag gttttatccc gttatcagga tcaccttctt
      181 tgggatcata aatgtatccg cataccgtgc attcatattt tttatccatg tagtagacct
      241 cctataaatt tatcttgtaa taaactacaa gccctcatgc taactgtgtg gtgcttttaa
      301 aaaaaacaac atggttatcc tcacatataa tacatcacca tgttataaca gttcaggtgt
      361 atagccagct tcttttatag cttgcatcac aatagatgca tctaaagagc tatcgaccat
      421 aaccattttg gtggttacat tacattgcac ctttgcagca ggatcttttt catttattgc
      481 ttttgtaata tgcattacgc agtgattgca catcatgtca ttaacacgga attgcattgt
      541 tactcctttt atccattgtt ttagtatggt acagtataaa acataaatta caagtaaaat
      601 ttaaaataat ctatctcaac atcgtctatc acatatagat atgtttatag aatattttga
      661 tgctattcat cgttaataat tgtagcatat ataatgtaat tatctcaaaa ttataataat
      721 tacaaaattt tgtcaagcaa tttataaaaa aagaattgta aaaagatttc agtataacta
      781 aaactttcag gaacaaaaga tttaatgtga tgagcactct ttacagatgc cttttaatgt
      841 gatttgatac tgttcaattg tatggccgtc tattgattca ggcatgttga cgtaacgggt
      901 aatattcagg tcatataccc ttccgcactg cgtgcaggta aaatgaccat gtggctgaac
      961 cgttatatca aaacgcgcct caacgttgtc aagaaccagc atgcttgcca ggccttcctg
     1021 aacaaaaaga ctcagtgtat tatatacagt agttttagaa agagttggta tatgtggcaa
     1081 aagcgctgta taaatctcat caacagttgg gtgcgttctg ttctggtcta aatactccag
     1141 aatcttcagt cgctgatatg atggcgaaat ccccttggct tccaggtaat gtttatattg
     1201 ttccattgtg gtcctcattg taataataaa ttggactcat tatgcaatta tataatacat
     1261 aatactgtag taaaagtcat cttaaattta taatttttca aaaacctgtt gggcgatagt
     1321 tcccagcaat ggttcatcag gtacatactg gaccttaatg gaaggaagca gttgccatcc
     1381 catttccgct aataccgctt caacctgtgc aacgctttgt ccgccccagc cgtatgatcc
     1441 aaatgcaagc cccacgcgat tctttggtgc aaggcccttg cagtaggtta aaaatgcagc
     1501 aacagttggc agcatattgt tgttaagtgt aggagagcca acacagatat actttgcatc
     1561 aagtacttcg gtcatgatat cagaaatatg gatggttttt aaatcataaa gctgtgcagg
     1621 aatatttttt tcctcaaagg ctttttttat tgcatgtgca atgagtttag ttgaattcca
     1681 catggtgtca taaatgatta acgctttttc ttttgtttta ttggcagccc agttgttata
     1741 ctcagtaact atcgccctta catgatcttt ccatataatc ccatggctgg gtgcaatcat
     1801 ggcaatatcc agtgtgctta ccgcatcaag cactttctga gtttgtttat catacggcaa
     1861 tacaatgtta gcatagtatt tttttgcctc atacatgatg atgtccagcg gatattctga
     1921 gtcaaagcgt tctgatgttg ctatatgctg tccaaaggca tcgttggaaa atagaatttt
     1981 agtttcaggc atataacagg ccatgttgtc aggccagtgt accatctgtt caaggatgaa
     2041 ttgcagcgtg gtttttccta acgagatact atcgcccgtc ttcatgactt tgtaattcca
     2101 gttctttttg taatgagcct ctaacccctt tgcgccattg ggtgatgtga tgagcgttgc
     2161 gttgggacac aactccagaa gttttggcag cccgcctgaa tggtccattt caacatgatt
     2221 agaaataata atgtcaatct ttgcaggatc aaccactttg gaaatgcgtt caatcatttc
     2281 atcatacaga tagtatttca cagtatcaat aagggtaatc ttttcatcca tgattacata
     2341 ggcgttatac gtagacccgc gttgtgtcat ataaccgtgg aagttgcgca aatcccagtc
     2401 aatgccgcca acccagtata taaagtcatt
//
LOCUS       DAOSZP010000352         2426 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_33446_length_2426_cov_3.174610, whole genome shotgun sequence.
ACCESSION   DAOSZP010000352 DAOSZP010000000
VERSION     DAOSZP010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2426
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33446_length_2426_cov_3.174610"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..974
                     /locus_tag="PK348_07880"
     CDS             <1..974
                     /locus_tag="PK348_07880"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HXK66170.1"
                     /translation="ITKPKYITLFGGEPLLDSPHARKIISYFIEKANQHSLHLAIVTN
                     GYTLSSYIDILKKATIREIQVTLDGTEDVHNARRFTKTKTPTFDAIVTGIDTALKHNI
                     PINLRVVVDAQNINDLPNLAQFAIDKGWTNNPLFKTQIGRNYELHYCQADNAKLYDRA
                     SLYEKIYELIIQHPHILQFHKPAFSISKFLFEQGAMPNPLFDACPGCKTEWAFDYTGK
                     IYPCTAMVGKKGEEVGTYYPTVSLNTTVIEEWEKRDVTSIPQCQQCSLSLLCGGGCAA
                     VAKNKHGNILQSDCRPVDTLLSMGLSLYFEKEIGNYHAKSCNNQKSM"
     gene            1023..2315
                     /locus_tag="PK348_07885"
     CDS             1023..2315
                     /locus_tag="PK348_07885"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXK66171.1"
                     /translation="MSEKIKYIDASQFEKEVLRGGNVIVDFYSTECPPCEALAAKFEP
                     LSEIFGDDITFIKIFRQENRALAESLGVTSSPTVLFYQNGREIGTRLSGSIKRSELLD
                     TIKLLLPQERYSQLLSAIQPVTTYTDILILGAGPAGLTAGIYTAQAKLNTIIVDTQLP
                     GGQVQTTHMVSNYPGFAKPVEGFMLMHYMSEQAKEAGCQFRVAVDVTDINLRKKYIIV
                     DGIETIHAKKIIVASGCSYRELNVPGEYEYKGKGISYCATCDAKYYQDKHVVVIGGGN
                     SAIEESLFIAKFARHITIVHQFDRLQANREAQEKAFASEKISFMFSHEPRAFIKNDDG
                     SMTVEVENLKTGNRSFIACDGVFIFAGMKPNMVNEDLQNNNGYIITDEFMHTSLPDVF
                     AAGDIRYKPYRQITTAVSDGTIAAITAVRELEEATVAV"
BASE COUNT          813 a          449 c          474 g          690 t
ORIGIN      
        1 atatcacaaa accaaagtac attaccctct ttggcggcga gccactgctt gattcacctc
       61 atgcacggaa aataatttcc tattttattg aaaaagcaaa tcagcattca ttacatttgg
      121 cgatagttac caacggatac acgctgagtt catatattga cattttaaaa aaagctacta
      181 tccgtgaaat ccaggtaaca cttgatggta cagaagatgt tcacaatgca cgaagattta
      241 caaaaaccaa aacaccaaca tttgatgcga tagttacggg tatagatact gcactgaaac
      301 ataacatacc cataaactta cgtgttgtcg ttgatgccca aaatattaat gaccttccaa
      361 acttagcaca gtttgccatt gataaaggat ggaccaacaa tccattgttt aaaactcaga
      421 ttggacgtaa ctatgaactt cactattgcc aggctgataa tgcaaaactt tatgacagag
      481 catcattgta tgaaaaaata tatgaactca ttatacagca tccacacatt ctgcaattcc
      541 ataagcctgc attctcaata agcaaatttt tatttgaaca gggtgctatg ccaaatcctt
      601 tatttgatgc atgccctgga tgcaagactg agtgggcatt tgattataca gggaaaatat
      661 atccatgcac tgcaatggta ggcaaaaaag gcgaagaagt tggtacatat taccccacag
      721 tgtcattgaa cactactgtc attgaagaat gggaaaaacg tgatgttaca tccataccac
      781 aatgtcaaca atgttctttg agtttactgt gtggcggtgg ttgtgcagcc gttgcaaaaa
      841 ataagcacgg caatatactg caatctgatt gcaggccagt tgatacattg ctttcaatgg
      901 gtttatcatt atattttgaa aaagaaatag ggaattacca tgcaaaatca tgtaataacc
      961 aaaaaagtat gtaaccagac tgccgaaagc ggcaaggtat atcaatactg aaggagtaca
     1021 tcatgagtga aaaaatcaag tatattgatg catcacaatt tgaaaaggaa gttctgcgtg
     1081 gtggcaatgt tatcgttgat ttctattcta ctgaatgtcc accctgtgaa gcattggctg
     1141 caaaatttga acctttgagt gaaatctttg gtgatgacat aacatttata aagatattca
     1201 ggcaggaaaa tcgcgcttta gctgaatcat taggtgtaac atcatcaccc actgtgttgt
     1261 tttatcaaaa tggcagggag ataggcactc gcttatcagg cagcataaaa cgctctgagc
     1321 ttcttgatac tattaaactt ttattaccac aggagcgtta ttcacagtta ctatcggcca
     1381 tacaacctgt aacaacatac actgatattc ttatcttagg tgcaggcccc gctggattaa
     1441 ctgctggcat ttatactgca caggcaaaac ttaacacaat tattgttgat actcagcttc
     1501 ctggtggtca ggtccagaca acacatatgg tttcaaacta ccccggtttt gcaaaaccag
     1561 ttgaaggatt tatgcttatg cactatatga gtgaacaggc aaaagaagca ggttgccagt
     1621 tccgtgttgc tgttgatgtt acagacataa atttaagaaa gaaatatatt atcgtagatg
     1681 gtatagagac tattcatgct aaaaagatta ttgtagcttc aggatgttca tacagagaac
     1741 tcaatgtccc gggcgaatat gaatataaag ggaaaggcat atcatactgt gcaacctgtg
     1801 atgctaaata ttaccaggac aagcatgtgg tagtgatagg tggtggtaat tccgcaattg
     1861 aggaatcact gttcattgct aaatttgcac gtcatataac tatcgttcat cagtttgaca
     1921 ggctgcaggc aaacagggaa gcacaggaaa aagcatttgc cagcgaaaag atttctttca
     1981 tgttttctca tgaaccacgt gcattcatta aaaatgatga tggcagcatg acagtggagg
     2041 ttgaaaacct taaaactgga aatagatcat tcattgcatg tgatggtgta ttcatatttg
     2101 caggcatgaa gccaaatatg gttaatgaag atttgcaaaa taacaatgga tatattatta
     2161 ctgatgaatt catgcacacc tcattgcctg acgtgtttgc agcaggtgat atacggtata
     2221 aaccatacag gcaaataaca acagcagtat ctgatggtac tattgctgca attactgctg
     2281 tcagggaact ggaagaagca actgttgcgg tataatgatt atttgacagg ctttgccacc
     2341 tggtaaagcc tgtcctcaaa aacttttaca tcaaattttt ctttttcaaa atcatccgga
     2401 aacaaataga gaattaaaag ccattc
//
LOCUS       DAOSZP010000353         2412 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_33706_length_2412_cov_3.061943, whole genome shotgun sequence.
ACCESSION   DAOSZP010000353 DAOSZP010000000
VERSION     DAOSZP010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2412
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33706_length_2412_cov_3.061943"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            356..943
                     /locus_tag="PK348_07890"
     CDS             356..943
                     /locus_tag="PK348_07890"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HXK66172.1"
                     /translation="MKNAQSFRELKESEKENRKKIIIDAAERVFATKPFNQVTMRDIA
                     KEAGITAGAIYRYFPDQQSLFVEAFLRGAEKIINDLEQVISRSKNPLKDTIATFLKFL
                     MKHDQYFRMMTHFMLDAHLNQDLVEKLNEAERRLLDQFTKLFEQRSHARLLAHSLFAA
                     MNGVVITFHNYPGRSKKDIHQHMNRLGDMLYQLFS"
     gene            complement(932..>2412)
                     /locus_tag="PK348_07895"
     CDS             complement(932..>2412)
                     /locus_tag="PK348_07895"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXK66173.1"
                     /translation="KVDQAARKLSKLASYRWRDIADTIAQPHNRIFMQHYDGHNHRID
                     RIVRPKELEEMEKEVFGQGLFSAKTLPWERLVKLILIYENGEAGIACPLVCTEGLVEL
                     LEKYHDHPKLEKILQHCKEGIDGEFAIGAQYLSEIQGGSDVAANDLEAVDNGDHWLLY
                     GKKFFCSATHADYVVITARPKGSEHVGLFVMPSWSDKTNEKRNGYTIDRLKWKLGTAE
                     LPTAEITFNGAIAYKAGPIERGIANVVGIVLTHSRLTVGLSAAAAMIRASREATQYAQ
                     FRNAFGFPINQFPLLKRQLDEINHYAQRTLAGAFKIYEMFQSLPGGLKGGLVTDEDVE
                     IQRKRFRVRILIMLQKIVASWDATDILRKAISVFGGHGVMEDFSSLPRLFRDMMINEL
                     WEGPRNVLLTQIYRDIQRASSWYPVHDFIHDLIYNANSKSINEIAASFAECLQSNFIY
                     PGQENDHLCIMWEHACHNLLHEFQNCALHEVEDAQMAISLRK"
BASE COUNT          756 a          462 c          447 g          747 t
ORIGIN      
        1 tatgctgaag agaaatacag gtagtagttt caaaatacat cgtttcctcc caaagaataa
       61 agatagttta tttaaaaaaa tatataaaat atacgttgtc aaatcatatt tttcttaacc
      121 atatttaaac aaatgtaagg ctaatgttgt tgcaatattt ttcacaatac agagtgatat
      181 taggatagga acttattaac tagtagacta aatgctaagt agcaaaaaga attaacatta
      241 gtaaatgtct ataatatgtg aataaaaaga agaaagttat attttaaaac gtttatgtta
      301 ccaccaatag attattaact tgacatgttt aatgaaaata taacatattt gctttatgaa
      361 aaatgcacaa tcatttcgtg aattaaaaga atctgaaaaa gaaaatagga aaaaaataat
      421 tattgatgct gcagaacgcg tatttgcaac aaagcctttt aatcaggtaa caatgcgtga
      481 tatagctaaa gaggcaggta taactgcagg tgccatttat cgctatttcc cggaccagca
      541 atcattattt gtggaagctt tcctgcgggg tgcagaaaaa ataattaatg atcttgaaca
      601 ggttatttcc cgcagcaaaa atccattaaa agatactatc gccacatttt taaaattttt
      661 gatgaagcat gatcagtatt tcaggatgat gacccatttt atgctggatg cacatttaaa
      721 ccaggatctt gttgaaaagc tcaatgaagc agaacgaagg cttttagatc aatttacaaa
      781 gttgtttgaa caaaggtcac atgccaggtt acttgctcac agtttatttg ctgcaatgaa
      841 tggcgttgtg attacgtttc ataattatcc tggcaggagc aaaaaagata tacaccagca
      901 tatgaacagg ttgggcgata tgctatacca gttattttcg taatgatata gccatctgtg
      961 catcttcaac ttcgtgaaga gcacagtttt gaaattcatg caaaagatta tgacatgcat
     1021 gttcccacat aatacaaaga tgatcgtttt cctgcccagg ataaataaaa ttactctgca
     1081 aacattccgc aaatgatgca gcaatctcat taattgattt actgtttgca ttgtaaataa
     1141 ggtcgtgaat gaaatcatgc accgggtacc atgacgaagc acgctgaatg tcgcgatata
     1201 tttgtgttaa aagcacatta cgcggacctt cccataattc atttatcatc atgtcacgga
     1261 aaagccgtgg caaacttgaa aaatcttcca taacgccatg cccgccaaat actgagattg
     1321 cttttcgtaa aatatcagtt gcatcccatg aagcaactat cttctgcagc atgataagga
     1381 tgcgcacccg gaaacgcttt ctttgaattt ctacatcctc atcggttaca aggccgcctt
     1441 ttaatccacc gggcagtgac tgaaacattt catagatttt aaatgcacca gcaagtgttc
     1501 gttgtgcata gtgattaatt tcatcaagct gtcgtttaag tagtggaaat tggttgatgg
     1561 ggaatccaaa tgcatttcgg aactgtgcat actgtgttgc ttcgcgtgaa gcacgaatca
     1621 ttgctgctgc tgccgataac cctacggtca gccgcgagtg tgttaaaact atgcctacga
     1681 cattggcaat gcctcgttct attggtccgg ctttataggc gatagctcca ttgaatgtaa
     1741 tctctgctgt gggaagttct gctgttccta acttccattt aagcctgtca atagtataac
     1801 cattacgttt ttcgtttgtt ttatcactcc atgatggcat gacaaaaagg ccaacatgct
     1861 ctgacccttt aggacgggca gtaataacaa cataatctgc atgtgttgca gaacagaaga
     1921 attttttgcc gtataaaagc caatgatcac cattatctac tgcttcaaga tcattggctg
     1981 caacatctga tcctccctga atttctgaga ggtactgcgc accaattgca aactcaccgt
     2041 caatgccttc tttgcaatgc tgtaagattt tttccagttt gggatgatca tggtattttt
     2101 ccagaagttc aaccagtcct tcggtacata ctaaaggaca cgcgatacct gcttcgccat
     2161 tttcatagat aaggattaat tttaccaggc gttcccacgg caacgttttt gctgaaaata
     2221 atccctggcc aaatacttcc ttttccattt cttcaagttc ttttgggcgt acaatgcgat
     2281 ctatcctgtg attatgccca tcgtaatgtt gcatgaaaat tctgttgtgt ggttgtgcta
     2341 ttgtatcagc tatatcccgc catctataag aagccaattt tgaaagctta cgggcagcct
     2401 ggtcaacttt tt
//
LOCUS       DAOSZP010000354         2409 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_33762_length_2409_cov_2.651232, whole genome shotgun sequence.
ACCESSION   DAOSZP010000354 DAOSZP010000000
VERSION     DAOSZP010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2409
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33762_length_2409_cov_2.651232"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..132
                     /locus_tag="PK348_07900"
     CDS             <1..132
                     /locus_tag="PK348_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014788979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015075 - monoatomic ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter complex subunit
                     F"
                     /protein_id="HXK66174.1"
                     /translation="IVIMIVLGAAQKKALYIDIGIVYGIISFIGTLYIARYLIDERK"
     gene            133..540
                     /gene="mnhG"
                     /locus_tag="PK348_07905"
     CDS             133..540
                     /gene="mnhG"
                     /locus_tag="PK348_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012572546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H(+) antiporter subunit G"
                     /protein_id="HXK66175.1"
                     /translation="MDTVIISCLVIGLVVNGMSIIGLMRFPDIYTRLHAATKTTTFGS
                     IFVVLAIVIKNIIYYDQASLTIAIHSIVALLVIIFTNPISAHAIARASLLSGIKPFGV
                     VINEFDYKSQIKTADELELEKVSEGIPDEDGVL"
     gene            537..779
                     /locus_tag="PK348_07910"
     CDS             537..779
                     /locus_tag="PK348_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015283646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4040 domain-containing protein"
                     /protein_id="HXK66176.1"
                     /translation="MMYYFFLVISIALVITALLSIYFRDLLAAIVSYSVFSLVLTVLY
                     FLLDAPDVAIAEAGIGAALTTCIFVIAIRMTRRREE"
     gene            776..1609
                     /locus_tag="PK348_07915"
     CDS             776..1609
                     /locus_tag="PK348_07915"
                     /inference="COORDINATES: protein motif:HMM:NF006248.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MnhB domain-containing protein"
                     /protein_id="HXK66177.1"
                     /translation="MRLLSVVAIVILFGFLFVGILHLHPVGEALGNSEVLQGRPELGI
                     DINGVSTMDDYFLRNGQIETKSNNIVASVVFDYRGFDTLGEATVLFIVVSSISMLFFT
                     FLKSKSIVPAGVPPAGQFPKIESRVITFGSFIMYIMILSFGVYLVVHGHISPGGGFQG
                     GSVMASATALLLISFLITRHMQRTRKIFSFFESLGLTIFIGLGFAGITLSFLYNFLAH
                     TSTGFGRIIAFGPNQGYLISAGILPLLSLAVGIEVFFGLSLIVVTLFHVSGVKDTNSL
                     R"
     gene            1615..1974
                     /locus_tag="PK348_07920"
     CDS             1615..1974
                     /locus_tag="PK348_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015283649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter subunit C"
                     /protein_id="HXK66178.1"
                     /translation="MSAIVLYVTFILLLTIGIIALLFKRNLIKIVLAFNILDSGINLF
                     FIALGYRENAMAPIFTLAPLESVITMVLPTPQALILTNIVIGFATTALMLGVSVLTYR
                     NNNTLDTRKLRGVEEYD"
     gene            1967..>2409
                     /locus_tag="PK348_07925"
     CDS             1967..>2409
                     /locus_tag="PK348_07925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66179.1"
                     /translation="MIKHVPVLLVAIPLLAAFMIPMIGMVNRTARTVVGAIALVMILA
                     ASYYIAGDILVHGRILYYMVGSSNPEMLTEHGLTFPIRIGLKIDPFSLFFIIMTTTLV
                     LIFYVFSAQYIEENERKNYFTVLFITMVAGMMGLLVANDLFTFFV"
BASE COUNT          649 a          403 c          505 g          852 t
ORIGIN      
        1 attgttatca tgattgtgct gggcgcagct cagaaaaaag ccttatacat tgatattggt
       61 attgtatatg gaattatctc gtttatcggg acattatata ttgcccgcta tctcatagat
      121 gagaggaagt aagtggatac tgttattata tcgtgccttg ttattggttt ggttgtcaac
      181 ggtatgagca ttattgggct catgcggttc cctgatatat atacccgttt gcatgctgcc
      241 acaaaaacaa ccacgtttgg cagtatcttt gttgttttgg cgatagttat taagaatata
      301 atctattatg atcaggcaag tcttacaatt gccatccaca gtattgttgc attgctggtt
      361 attatcttta ccaatccaat cagtgcccac gcaatagcac gagcatcatt gctatcgggc
      421 attaaaccgt ttggtgttgt tataaatgag tttgattata aatcccagat taagactgca
      481 gatgagctgg aacttgaaaa ggtaagcgaa ggaattcctg atgaggatgg tgtactatga
      541 tgtattactt ctttctggtg atttcaattg cactggtgat cacagcattg ctttcaatct
      601 attttagaga tcttctggca gctatagtaa gctattcggt tttttcactg gttttgacgg
      661 tgctctattt tctgttggat gcacctgatg ttgctattgc agaagctggt attggagcag
      721 cactcacaac ctgtattttt gttattgcca tcagaatgac acggagaagg gaagaatgag
      781 gttattgagc gttgtagcta ttgttatact attcggattt ctttttgttg gaatattaca
      841 tctgcatcca gttggagaag ctttaggaaa ttcagaagtg ttacagggaa ggccagaact
      901 gggtattgat atcaatggtg ttagcactat ggatgattac tttttgcgca atggtcagat
      961 tgaaacaaag tcaaacaata ttgttgcaag tgtggtattt gattaccggg gatttgacac
     1021 attaggggaa gccacggtgt tatttattgt ggtaagcagt atttcaatgc tcttctttac
     1081 atttttaaaa agcaaaagca ttgttccagc aggtgttcct cctgcaggtc aattcccaaa
     1141 aattgaatcg agggttatta catttggatc ttttatcatg tatatcatga tattaagctt
     1201 tggggtttat ctggtggttc atggtcatat ctcaccggga ggaggtttcc agggaggatc
     1261 ggttatggct tcggcaacag cacttttact tatcagcttt ttgattaccc gccatatgca
     1321 acggactcgt aaaattttct cgttctttga atcattggga ttaaccattt ttattggatt
     1381 gggatttgca ggtatcacac tatcgttttt atataatttt cttgctcaca ccagtactgg
     1441 atttggcagg attatagctt ttggaccaaa tcaaggctat ttaatatcgg cgggaatatt
     1501 accgttgcta tcactggctg taggtattga ggtatttttt gggttaagcc ttatcgttgt
     1561 aacattattt catgtatcag gggtaaaaga tactaattca ttacggtaat tattatgtca
     1621 gcaatcgtgc tttatgtaac atttattctg ttattaacca taggtataat agcactgtta
     1681 tttaaacgca atcttattaa aattgtactg gcatttaata tcctggattc aggtataaac
     1741 ctgtttttta tcgctttagg ataccgtgaa aatgccatgg ccccaatatt cacactggcg
     1801 ccattagagt ctgttataac tatggtattg cccacacctc aggcgctaat attaaccaac
     1861 atcgttattg gatttgctac cactgcactc atgttaggag tttctgtgct tacatacaga
     1921 aacaacaata ctctggatac ccggaaattg cgtggagtgg aagagtatga ttaaacatgt
     1981 tcctgtatta ctggtagcta tcccacttct tgctgctttc atgataccaa tgattggtat
     2041 ggttaatcgt actgcacgta ctgttgttgg ggcgatagct ctggtaatga tactggcagc
     2101 atcatattat attgctggtg atatcctggt tcacggtaga attttgtatt acatggttgg
     2161 atcatccaat ccagaaatgt taacagaaca tggattgaca ttcccaatac gcataggatt
     2221 aaaaatagac ccattttcac ttttttttat tataatgaca actactcttg tgttaatttt
     2281 ttatgtattt tccgcccaat atattgaaga aaatgaaaga aaaaattatt ttaccgtgtt
     2341 attcataact atggtggcag gcatgatggg gttacttgtt gcaaatgatc tgtttacctt
     2401 ctttgtttt
//
LOCUS       DAOSZP010000355         2409 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_33763_length_2409_cov_2.389975, whole genome shotgun sequence.
ACCESSION   DAOSZP010000355 DAOSZP010000000
VERSION     DAOSZP010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2409
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33763_length_2409_cov_2.389975"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..801)
                     /locus_tag="PK348_07930"
     CDS             complement(<1..801)
                     /locus_tag="PK348_07930"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXK66180.1"
                     /translation="MKLSYKISPINDTIFRISLPLPGYPPYINVYLFKGEVNTLFDTG
                     IFWTWRLLRRALHDLGLQFSDIHQVIPSHGHVDHYGAAYAIAKRNKHVEVITHIADAK
                     RIEEGLYNKPSIMLKFIESMGVPKPLIVPLAFLDLTFKSMGHPVIVTRKITDDQCSLQ
                     VGNYNATLILTPGHSKGAMCLYIEKEGFLFSGDHIVPGVKPIILAGFEPNTSFPVAQS
                     HTIFHTSLKKIEKLSPQYIFPAHGEPFQEKLNRLIVKYWIVISNQQQNQ"
     gene            complement(1106..>2409)
                     /locus_tag="PK348_07935"
     CDS             complement(1106..>2409)
                     /locus_tag="PK348_07935"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF007746.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HXK66181.1"
                     /translation="PRLPQWVHDGMWIGMQGGTDVVKKKLQVALDAGVKVSALWVQDW
                     VGRRITWFGKRLFWNWKYNQELYPGLPSYIQELSHKNIKFLGYINCFLNSEGDLYKEA
                     ISKGYLVHNQDGTVKTFDTTGIIAGTLDLTNPQTREWIKNVIKEHMIGIGLGGWMADF
                     GEYLETDAKLYSGESAELYHNKYPADWARVNYEALEEAGKLGEVVFFTRAGYTGTSKY
                     STLMWAGDQLVNWSMHDGLASVIPAGISLGFCGVGYFHADIGGYTTLGWIKRKKELFM
                     RWAELGAFTAVMRTHEGNRPDVNLQFNSDMETLQHLAKMTKVHTILKPYFEHCKDEYH
                     TDGLPLMRHPYIHYPDDEKLHSLQYQFLLGRDVLVAPVYRPRRKKWNVYLPKDEWVHV
                     WSGKEYSGGWVKVEAPLGQPPVFYRKKSQFKTIFEAIKDIK"
BASE COUNT          728 a          499 c          396 g          786 t
ORIGIN      
        1 ctgattttgc tgctgatttg atatgacgat ccaatatttt actatcaaac gatttaattt
       61 ttcctgaaaa ggttcaccat gtgcaggaaa aatgtattgg ggacttaatt tttcaatctt
      121 tttcagtgag gtatgaaaga tagtatgact ttgggcaact ggaaatgatg tatttggttc
      181 aaagcctgcc agaataattg gctttacacc aggtacaatg tgatcaccag aaaataaaaa
      241 cccttctttt tcaatataca aacacatagc acctttggaa tgtcccggtg ttaagatcaa
      301 tgtagcattg taattcccaa cctgtaatga acattgatca tcggtaattt ttcgtgtaac
      361 aataacggga tgacccattg atttaaatgt taaatcaaga aatgccagtg gcactattaa
      421 tggcttaggt acacccattg attcaatgaa tttcaacata atagaaggtt tattgtacaa
      481 gccttcttct attcgtttag catctgcaat gtgagttatt acttcaacat gtttatttct
      541 tttagctatt gcatatgctg caccatagtg atcaacgtgt ccatgtgagg gaataacttg
      601 atgaatatca gaaaattgta gtcctaaatc atgcaatgct cttcgcaaca accgccatgt
      661 ccagaatatt ccggtatcaa atagtgtatt aacttctccc ttaaaaagat atacattgat
      721 ataaggtggg taacccggga gaggtaatga aattctgaaa atggtatcat taattggtga
      781 aattttatat gaaagcttca atggcatcct gttgtcatta aagaattttt cattaatata
      841 tctaatttta ctaatgattc cttaaaattt ttacaattga ttgtgaaata aataacttta
      901 agaattatta atttataaat ttataaaaat tattaaatta tatagctgtc aattgtttta
      961 tcttagaaaa ttaatgcatt gtaataacat gaaatgaata attgttgact tatagcacat
     1021 atatagtatg ggcatgtttt tctgattgtc atagtttcag atcacaatgg ggacaggcga
     1081 aaattgttac aatagctact gtatcttatt tgatatcttt tattgcctca aaaatagttt
     1141 taaactgtga ttttttcctg taaaaaacag gtggttgtcc taatggggct tcaaccttaa
     1201 cccatccacc actatattcc ttccctgacc atacatgtac ccattcatcc tttggaagat
     1261 atacgttcca cttttttctt cgtggcctgt acacaggcgc taccagaaca tcacgaccca
     1321 gtaaaaattg atattgcaac gaatgaagct tttcatcatc aggataatgg atgtagggat
     1381 ggcgcatcag cggtaatcca tcagtatgat actcatcttt acaatgctca aaatatggtt
     1441 ttaagatggt atggactttt gtcatttttg ctaaatgctg cagcgtttcc atatcactgt
     1501 taaattgcaa gtttacatca ggcctatttc cttcatgtgt cctcattact gctgtgaacg
     1561 cacccagctc tgcccagcgc ataaaaagct ctttctttcg cttaatccag cccaatgtgg
     1621 tatatccacc aatatccgcg tgaaaatatc ccacaccgca aaatcccagt gaaatacctg
     1681 caggtatcac cgaagccagt ccatcatgca tgctccagtt aacgagttga tccccagccc
     1741 acatcaatgt gctgtattta gatgtacctg tatatcctgc ccgtgtaaaa aatactacct
     1801 cacccagctt acctgcttct tctaatgctt cataattgac acgtgcccaa tctgcgggat
     1861 atttattgtg gtacaattca gcagattccc ctgaataaag ctttgcatcg gtttccagat
     1921 attcaccaaa atcggccatc cagccaccaa gaccaattcc aatcatatgc tctttaataa
     1981 catttttaat ccattcccgc gtttggggat tggtcaaatc taacgtgcca gcaattattc
     2041 cagtagtatc aaacgtttta actgtgccat cctgattgtg taccaaatac cctttggaaa
     2101 tagcctcttt atacaggtca ccctcagaat ttaaaaagca gttaatatat cctaaaaatt
     2161 ttatattttt gtgcgatagt tcctgtatat atgatggcag gcctggatac aattcttgat
     2221 tatatttcca gttccagaaa agcctcttgc caaaccaggt tattcgtcgg ccaacccagt
     2281 cctgtaccca cagcgctgaa accttaactc ccgcatcaag tgcaacctgt aattttttct
     2341 tcacaacatc ggtgccaccc tgcattccta tccacatgcc atcatgcacc cactgaggta
     2401 gccgtggct
//
LOCUS       DAOSZP010000356         2408 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_33784_length_2408_cov_2.660433, whole genome shotgun sequence.
ACCESSION   DAOSZP010000356 DAOSZP010000000
VERSION     DAOSZP010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2408)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33784_length_2408_cov_2.660433"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1624
                     /locus_tag="PK348_07940"
     CDS             <1..1624
                     /locus_tag="PK348_07940"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HXK66182.1"
                     /translation="VERKPNAIAWDFMGYTATYKEFAQLIDRCAEGLYALGLKEGDTI
                     TISMPTTPQGIICFYAVNKIGAVASMIHPLSPPREIEFYLTISNSTFALTIDAFYGAF
                     KEVMDKTPCKTLILTKISDFLPGLKALGFYLTKGRKIPKVPKDPRVVWWSDMMKKSSK
                     VPKSSMKTDDMAVILYSGGTTGMPKGIMLSNMNFISEGMQVASWGKMSEDDSILAILP
                     IFHGFGLGVCTNAFFMGGGKSILVPQFDPVIVAKLIKQKKPNFLIGVPTLFEALSKSK
                     VFGSADLSCIKAAFSGADTLPRVVKEEFERVVARQGGNVKLREGYGLTEAVTAIMAMP
                     MNEYREGSIGIPFPDMMAKIVKQGTTEEAPCGEEGEICVAGPAVMLGYLNQPEETAKT
                     LRKHDDGLVWLHTGDIGTMDEDGFFYFKLRLKRMIKSSGFNVYPSQVEDVLRKHPAVD
                     QACVIGVPDKAQVERVKAFIVLKPEYKDKAGSALAEELIGHCREHLIKWSCPREIEFR
                     DELPKTKVGKIAFNVLEEEEKEKLRSRGEYAG"
     gene            1643..>2408
                     /locus_tag="PK348_07945"
     CDS             1643..>2408
                     /locus_tag="PK348_07945"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HXK66183.1"
                     /translation="MNRLSIYIFEFAFRFPWLRYIVWAIIVSVSIYAGYHLFYEEVVT
                     YPVGWERSAVITPYTLKVKSPFAVSKGNVVAVVFEGTEKNEHGIYIILSLNGGKDFLP
                     PYKLSDIDPAVAVRPHVAISNNGHIACTWHNLVDSKSNIFLTTSNDLGATWTKPVALL
                     LQTDESMLSQVFYDSTGGLHLFYHGSVGGRLILYHAFSPDELQFTRIDTIADIATEYR
                     GIFFPAIVFEGDSIYLVFQAKILSGGVLSDDLFFVYS"
BASE COUNT          719 a          402 c          574 g          713 t
ORIGIN      
        1 agttgagcga aaacccaatg ctatcgcctg ggactttatg gggtatacag ccacgtacaa
       61 agagtttgca caactcattg ataggtgtgc tgaagggtta tatgcattag ggcttaaaga
      121 gggtgatacc ataactatct caatgccgac aacgcctcaa gggataattt gcttttatgc
      181 tgtcaataag attggggctg tggccagtat gatacatcca ctatcgcctc ccagggaaat
      241 cgaattttat cttactataa gcaacagtac atttgccctt accattgatg ccttttatgg
      301 tgcttttaag gaagttatgg ataaaacccc atgtaaaacc ttaatactta ctaaaatttc
      361 agattttttg ccagggctta aagcattggg gttttattta acgaaaggga ggaagatccc
      421 aaaagtgccc aaagatccgc gtgtggtttg gtggtctgac atgatgaaaa agtcaagtaa
      481 agttcccaaa tccagcatga aaactgatga catggcagtt attttataca gtggtggcac
      541 tacaggtatg cccaaaggaa ttatgctttc aaatatgaat tttatcagtg aaggcatgca
      601 ggttgcttca tggggaaaaa tgtctgaaga tgattcaata ctggcaatat tgcctatatt
      661 ccacggattt gggctgggtg tttgtaccaa tgcatttttt atgggtggtg gaaaaagtat
      721 tctggtacca caatttgatc cggttattgt tgcaaaactt attaaacaaa agaaacccaa
      781 tttccttatt ggtgtcccaa cgttgtttga ggcattgagt aaaagcaagg tgtttggttc
      841 tgcagattta tcatgtatta aggcggcttt cagtggtgca gataccctgc ctcgtgttgt
      901 aaaggaagaa tttgaaaggg tagtagcacg gcagggtggt aatgtaaaat tgcgtgaagg
      961 gtatggtcta accgaagctg taacagcaat tatggcaatg ccaatgaatg aatatcgtga
     1021 aggtagtatt ggcattcctt tccctgatat gatggcaaag attgttaaac agggcacaac
     1081 tgaggaagct ccatgtggtg aggaaggtga aatatgtgtt gctgggcctg ctgtaatgct
     1141 gggttacctt aatcagccag aagaaactgc aaaaacgctg cgcaagcatg atgatggttt
     1201 ggtatggtta cataccggtg atatagggac aatggatgag gatggatttt tctatttcaa
     1261 attacggtta aaaagaatga tcaagtcatc gggatttaat gtatacccat cacaggttga
     1321 agacgtattg cgtaaacatc cagcagtaga ccaggcctgt gtaataggtg taccggataa
     1381 agcacaggta gaaagggtaa aagcatttat agttttgaaa ccggaatata aggataaagc
     1441 gggtagtgca ttggcagaag aattaattgg acattgcagg gaacatctga taaaatggag
     1501 ttgcccacgg gaaattgagt tcagagatga attgccaaag acaaaagttg gcaaaattgc
     1561 ttttaatgta ctggaagaag aggaaaagga aaaattacga agccgtggag aatatgctgg
     1621 ataaacttcc gataatagga gtatgaacag actttctata tatatttttg aatttgcatt
     1681 tcgattcccg tggctgcggt atattgtctg ggctattatt gttagtgtaa gtatctatgc
     1741 aggttaccat ctcttttacg aagaggtggt aacctatcca gtgggatggg agcggagtgc
     1801 tgtcataaca ccatatacac tgaaagtaaa gtcccccttt gcagtgtcaa agggcaatgt
     1861 cgttgcagtt gtatttgagg gcacggaaaa aaatgagcat ggtatttata ttatactttc
     1921 acttaatgga gggaaggatt ttctaccacc atataaatta tcagatattg accctgctgt
     1981 agctgtacgc cctcatgttg ccatatcaaa taacggtcac attgcctgta cctggcataa
     2041 ccttgttgat agtaaaagta acattttcct gactacttcc aatgatctgg gtgccacatg
     2101 gacaaaacct gtagccctat tgcttcagac ggatgaatcc atgttgtcac aggtgtttta
     2161 tgattccacg ggcgggctgc acctttttta tcatggttca gtaggtggtc gtcttattct
     2221 gtatcatgca ttttcccccg atgaactgca atttacaagg atagatacaa tagcagatat
     2281 tgcaacggaa tatagaggta tattcttccc tgctatagtt tttgaaggtg attcaatata
     2341 tttagtattt caggcaaaga tattgtccgg aggggtatta tctgatgacc tgttctttgt
     2401 ttattcga
//
LOCUS       DAOSZP010000357         2406 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_33807_length_2406_cov_3.827308, whole genome shotgun sequence.
ACCESSION   DAOSZP010000357 DAOSZP010000000
VERSION     DAOSZP010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2406)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2406)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2406
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33807_length_2406_cov_3.827308"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..343)
                     /locus_tag="PK348_07950"
     CDS             complement(<1..343)
                     /locus_tag="PK348_07950"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HXK66184.1"
                     /translation="MSKNECIITEVKDSVGLVYIDRTEVMNALNRTLIVELGNKIKDM
                     CADAGVRAIIISGKGDHFAAGADIGEMAQQNPHQALAFSFNDVYTMIEQLPVPTCAAI
                     KGYALGGGLELA"
     gene            complement(353..1513)
                     /locus_tag="PK348_07955"
     CDS             complement(353..1513)
                     /locus_tag="PK348_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012176514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HXK66185.1"
                     /translation="MGVRAIKKVTVIGSGAMGSGIAEVHIVNGFNTVMVDINNDLLQK
                     AKEKIKSNLSFLVNKQKMTQVAMDEAMARLATSTDITSSVKDADCIIEAVPELMDLKK
                     KIFKTVSDAARTDAIIASNTSSLSISELAETVSEPSRFAGFHFFNPVNRMRLVEVIYG
                     QKTSDEVIDTLMDLGEKLGKVAIKVLRDRPGFIVNRINAPLQTLWSAILDEGKISPAS
                     IDTVMKNHGAKMGPFELMDFVGLDVIYNVMQYYKTTLSPEWEPGKFITECVKKNELGM
                     KTGKGIYVWEGGKAIIDSSIVTDIIKPIDPLAVQLNEAVRVLKEKVAASAEDIDKGQE
                     AGMNQPGPFKTAMSIDNKLLAERLNWMSKTYNLTYLKPEPEILDGSFKSFMR"
     gene            1982..>2406
                     /locus_tag="PK348_07960"
     CDS             1982..>2406
                     /locus_tag="PK348_07960"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HXK66186.1"
                     /translation="MRKPRFIAPNITYHVTSRIIECRNMMEEDSIKALFEYCITLALE
                     KYEFELINYQIMDNHIHLLIRTVEGGASISRIVQYIKSRFAQRFNRMMQRTGPVWNER
                     FKDSIVEFADNPRMYLFNLLWYFANNPVRAGKVKSPFES"
BASE COUNT          689 a          543 c          379 g          795 t
ORIGIN      
        1 atgccaattc cagaccccca cccagcgcat atcctttaat ggcagcacac gtgggaacag
       61 gaagttgttc aatcatggta tacacatcat taaaactgaa agccaatgcc tggtgggggt
      121 tttgctgtgc catttcacca atatcagcac ctgctgcaaa gtgatcgcct ttaccagaaa
      181 tgatgatggc tctgacacca gcatcagcac acatatcctt aatcttgtta cctaactcca
      241 caattaacgt tctattaaga gcattcatga cttcagttct atcaatatac acaagcccaa
      301 ctgaatcttt cacttcagtg ataatgcatt cgttcttact cataccttcc ctctatctca
      361 taaatgattt aaagctacca tccagtattt caggttcagg tttaagatat gtcagattat
      421 atgttttact catccagttc agccgttcag ctaaaagctt attatcaata ctcattgctg
      481 ttttgaacgg ccctggctga ttcatgccag cttcctgccc tttatcaata tcttcagcac
      541 ttgcagctac tttttcctta agaacacgga cagcttcatt aagctgaaca gctaatgggt
      601 caattggctt tatgatgtct gttactattg atgaatcaat aattgcttta ccgccttccc
      661 atacataaat gcccttgccg gttttcatgc ccaattcgtt tttctttaca cattcagtta
      721 taaattttcc tggctcccat tcaggtgaca gtgttgtttt ataatattgc ataacattat
      781 aaataacatc cagaccaaca aaatccataa gctcaaaagg gcccatcttt gctccgtgat
      841 ttttcattac agtgtcaata cttgctggag aaatttttcc ttcatccaga attgcactcc
      901 acaaagtttg tagcggagca tttatcctgt ttacaataaa tccaggccta tcacgtaaaa
      961 ccttaatagc cactttaccc aacttttcac ctaaatccat gagtgtatcg atgacttcat
     1021 ctgatgtctt ctgaccataa ataacctcaa ctaagcgcat tctattaaca gggttaaaga
     1081 aatggaaacc agcaaatcgt gaaggctctg acaccgtttc agccaattcg ctaatactca
     1141 gcgaagaagt attactggca ataattgcat ccgtcctggc tgcatcagat actgttttga
     1201 atattttttt ctttaaatcc atgagctctg gcactgcttc aataatgcaa tcagcatcct
     1261 tcactgacga tgtaatatca gtagaagtag caagccttgc cattgcttca tccatagcta
     1321 cttgtgtcat cttctgttta ttaaccagaa aagaaaggtt tgacttaatt ttttctttgg
     1381 ccttctgtaa caaatcattg ttgatatcaa ccattactgt gttaaatcca ttaacgatgt
     1441 gtacttcagc aataccacta cccatagcac ctgagccaat aacagttact tttttaattg
     1501 ctctgacacc cataatgcct ccaataaaat cagctaaaat attatgtata agttaatgaa
     1561 taaccattaa cttatacata aatttttgtc aagcatttca cctaattttt aattcattta
     1621 taataataaa atttactatg cagaatggct ctgtcaccca tgggcgatag ttaccgatga
     1681 ttaaaccttt ccattttttc ttgcttcttt tctttttttg ctctgacact gtatgccagt
     1741 gcaccttctt acacggagtt tgcatattgc cgtgatgatg ttgcaatgaa cgtctcttcc
     1801 cgtgtgacaa ccactattgc tctgacacca tatatccttt aagatatgct ctgacactga
     1861 gttttaaatt tttccctatt tttctgaaaa atttttatca cttcctgcac tattttttgc
     1921 cttcatgcgt tgtatataca caaaagcgtt ttttacccta tttactttgg gaggaattac
     1981 catgcgaaaa cctcgtttca ttgcaccgaa cattacctac catgtcacta gccgcatcat
     2041 tgaatgcaga aacatgatgg aagaagactc tatcaaagca ctgtttgaat actgcattac
     2101 gctggctctg gaaaaatatg aatttgaact catcaactat cagattatgg ataatcacat
     2161 tcatctgctt attcgtactg tggaaggtgg tgcttccata tcccgtattg tgcagtatat
     2221 aaagtcacgg tttgcacaac ggtttaatcg catgatgcag cgaaccggtc ctgtatggaa
     2281 tgaacgtttt aaagacagca ttgtagagtt tgcggacaat cctcgtatgt atttattcaa
     2341 cttactctgg tattttgcca acaatccggt gcgtgcaggc aaggtgaaaa gtccttttga
     2401 aagcta
//
LOCUS       DAOSZP010000358         2403 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_33867_length_2403_cov_3.102641, whole genome shotgun sequence.
ACCESSION   DAOSZP010000358 DAOSZP010000000
VERSION     DAOSZP010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2403
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33867_length_2403_cov_3.102641"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(358..1884)
                     /locus_tag="PK348_07965"
     CDS             complement(358..1884)
                     /locus_tag="PK348_07965"
                     /inference="COORDINATES: protein motif:HMM:NF025925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HXK66187.1"
                     /translation="MNTKKVTLTILPHNTAFSVDAGITIADALLEYGIVINTPCGGKG
                     ICKKCAVKATGALSKPDVFEKSLGDSNYRLACRATLEGNVVVLADSIREYVHYPALNP
                     THTYGVAVDIGTTTLQIAFVDMTTDTVLPEITLLNPQRRFGHDVITRISHASDTTIAH
                     KLTQLLLDAIISIITTACRLSRAETNIVKAIAFSGNTVMSYSLSGIDITPLGVYPYRT
                     PVTTFDDYRNYRPFKEKFPEATIIIMPVVSAFLGGDFLGGLGTLSQTGGNSFFFDIGT
                     NGEMAIIKHDGSILATSCAMGPALEGMNISSGMTATEGAITHFMLENGKITFTAIGPP
                     TGISGTALVDLIAILLEIGVIDNSGKINTIPDALANQIAIEHNDTIKTLNILNTITLS
                     QLDIRNVQLAKGASLAASRILLKEAGLTEHDIDTVYIAGAFGKNVNIVNFTRLQFLPH
                     FTNAHFYIAGNTSLLAAIEAIRNKDFIQKLAAIRSTITAIDLSLHQEFNDLYVQSLNF
                     "
     gene            2094..>2403
                     /locus_tag="PK348_07970"
     CDS             2094..>2403
                     /locus_tag="PK348_07970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66188.1"
                     /translation="MSMDATIISYPDTAVINLLSLTANRSKYMIPFAGKFRVVDFVIG
                     NARELDAQTIIYSNVNDDLAQYVALHGIDESKLKVVVNEFNSIDFCYSLIRDSDSAYF
                     I"
BASE COUNT          729 a          399 c          496 g          779 t
ORIGIN      
        1 caaaaaggaa ttattgtggt tcaaagttat gagtggtaat taaaatagca gcgggatatg
       61 gcgtgccgta tattcataga aatatgcggt gtactgtggc gatagtacct gttaatggta
      121 gtcctggaat tgtttttatg aaagaatttg attgaattgt gtatcataaa aaaataaatt
      181 aatttgataa accgaattat cttttatagt taaaaaaatt tgttctaatt tgataaatta
      241 tttcaagaca aaaaagccaa agaaaaatga aaaactaaca tttgagattg taaaaatcag
      301 ggatgatggc ggacaggtaa gcaaaactac tgtgataaaa gatctgattg taaaaaatta
      361 aaaattcagt gattgtacat acaggtcgtt gaattcttga tgcagtgata aatctattgc
      421 agttattgta cttctgattg cagcaagctt ctgtataaaa tctttgttgc gtattgcttc
      481 aatagctgcc agaagcgatg tgttcccagc aatataaaag tgtgcatttg taaagtgtgg
      541 taaaaattgt aaccgggtaa aatttacaat gtttacgttt ttaccaaatg caccagctat
      601 atacacagta tcaatatcat gttcggttaa gcctgcttct tttaagagga tacgtgaagc
      661 agccaggctt gctccttttg ccagctgtac gttgcgtatg tcaagctgtg atagtgtaat
      721 tgtgttcagg atattgagtg tctttatggt atcattgtgt tctattgcaa tctgattggc
      781 caatgcatca ggaattgtat taattttacc gctattgtct ataacgccaa tctccagtaa
      841 aatggcaatt aaatccacca gggctgttcc actaatgcct gtaggtggcc ctattgcggt
      901 aaaggtaatc tttccatttt ccagcatgaa atgagttatt gcaccttcag ttgcggtcat
      961 accgctagat atgttcatac cttcaagtgc tggtcccatg gcacatgatg ttgccagtat
     1021 gctaccgtcg tgttttatga ttgccatttc accattggtg ccaatatcaa aaaagaaact
     1081 gtttccaccg gtttgcgaca gtgtaccaag cccacctaaa aaatccccac ctaaaaatgc
     1141 gctcacaacg ggcataataa ttattgtagc ttctggaaat ttttctttaa aggggcgata
     1201 gttccggtaa tcatcaaatg tagttacagg tgttctgtaa ggatatactc caagcggtgt
     1261 gatgtcaatg cctgataatg agtagctcat aacagtattg ccggaaaaag caattgcttt
     1321 tacgatgttt gtttctgctc tggatagacg gcaggctgtt gtgataattg atataattgc
     1381 atctaacaga agttgtgtaa gtttgtgtgc aatggttgta tcgcttgcgt ggcttatgcg
     1441 ggtaatgaca tcatgaccaa accttcgctg tgggttaagc agtgtgattt caggaagcac
     1501 agtatcggtt gtcatgtcaa caaatgcaat ctgtaaagta gtggtgccaa tatcaacagc
     1561 aacaccgtat gtatgtgtgg gattaagagc aggatagtgt acatattcac ggatgctatc
     1621 tgcaagtact actacattgc cttctaatgt tgcgcggcag gctaatctat agttactatc
     1681 gcccaaactt ttttcaaaca catcaggctt tgaaagcgca cccgtggctt ttaccgcaca
     1741 ttttttacat attcctttac caccgcacgg tgtattgata acgataccgt attcaagcaa
     1801 cgcgtcagca atggtaatgc ccgcatcaac agagaaagca gtattatgtg gaagtatggt
     1861 aagggtaacc ttttttgtgt tcatgatatc agttgattat tcaaatagtt ccatagaggg
     1921 catagtacag gtatgtttca ataaagtaaa tattttttca tatttatagt attcagatat
     1981 caaaaattat gggatatggt tgttacttta aattattttt ttatttacag atgaaaagtc
     2041 cttgctatga aacaacagta tcacataatg ttgctttgta aggagtaatc gaagtgtcaa
     2101 tggatgcaac tatcatatca tatccggata cagctgttat taatttacta tcgcttaccg
     2161 caaacaggtc aaaatatatg attccgtttg ccggtaagtt ccgcgttgtt gattttgtca
     2221 taggaaatgc aagggaactg gatgcacaaa ccataatcta tagcaatgtg aacgatgatt
     2281 tggcccaata cgttgcgctg catggtattg atgaaagcaa gcttaaagtt gtggttaatg
     2341 aattcaacag tattgatttt tgctattcgc ttattcgtga tagtgattca gcatatttta
     2401 taa
//
LOCUS       DAOSZP010000359         2401 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_33909_length_2401_cov_2.515345, whole genome shotgun sequence.
ACCESSION   DAOSZP010000359 DAOSZP010000000
VERSION     DAOSZP010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2401
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33909_length_2401_cov_2.515345"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /locus_tag="PK348_07975"
     CDS             <1..248
                     /locus_tag="PK348_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020263939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HXK66189.1"
                     /translation="GSVVVEKAKQEKQGVGFNAATMEWEDLMKAGIIDPAKVVRSALQ
                     NAASVAGMLATTEVLITEKKEEEKAGMPGGMPPGGMY"
     gene            complement(291..1250)
                     /locus_tag="PK348_07980"
     CDS             complement(291..1250)
                     /locus_tag="PK348_07980"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012674230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HXK66190.1"
                     /translation="MKAHRANKPYLSIILPVYNEEENIVPQYQHIVEAMQKTSYTYEI
                     IFVDDGSTDNSFKLLESIAKKDKHVHVVQFRRNFGQTAAMAAGIDYSKGDIIIFMDSD
                     LQNDANDIPLLVQKIEEGYDVVSGWRKNRQDKLLSRKIPSRIANWLIAKVTGVYLHDL
                     GCSLKAYRGEIIRQVNLYGEMHRFIPVHASWIGASIVEVPVHHHPRKFGKSKYGIKRT
                     FKVLIDLATVKFMGSYSTKPNYIFGGIGMILLFISILSGITVIAMKLFLHHSMIRNPL
                     LLLTVMLIILGIMFILLGILAEIMIRIYHESQKLPPYRVKRYI"
     gene            1383..>2401
                     /locus_tag="PK348_07985"
     CDS             1383..>2401
                     /locus_tag="PK348_07985"
                     /inference="COORDINATES: protein motif:HMM:NF017828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HXK66191.1"
                     /translation="MDKSTVIETIQQELDNLFARFSEIKQGGKKIIFTQCDSVPYEVF
                     VAHGFIVSKLPRWVMTALCKPDSSMYKRAVEYCSLADVLIVPQYCTIIPREVLQQLTV
                     HAIQHYEGFAQDAVVNLHTILYLVLQFLGVEQEYPDDDSLKQAVAVYEEIRKIMRALT
                     TMYAAVFTQSQLQLLCDAAFSLVPQQSLQLLQQLYHAVESSTHSDNEPTQKALVYSDF
                     SNWDIYDACNEFDIVIVEDDSCNGRRQFDISCHTSSQNLYYELLYAYSFKSWCPGMRT
                     VHERCELIYKALPNYDIKLVIFIQSFLNSRNEHLQSLYEQCLLQGTDAVILQADAITR
                     QLSHY"
BASE COUNT          735 a          450 c          503 g          713 t
ORIGIN      
        1 aaggatctgt tgttgttgaa aaggcaaaac aggaaaaaca gggtgttgga ttcaatgctg
       61 caacaatgga atgggaagac cttatgaaag ccggtattat tgacccagca aaggttgtcc
      121 gttcagcatt acagaatgca gcatcagtag ctgggatgct ggcaacaact gaagtgctta
      181 tcaccgaaaa gaaggaagaa gaaaaagcag gaatgcctgg tggaatgcct ccaggaggaa
      241 tgtactaaga tagttacagt aaaggcaggt tttaaacctg cctttacttt ttatatgtat
      301 cgttttacac gatatggtgg caatttctgt gattcatgat aaattctaat cataatttct
      361 gctaatatac caagtaatat aaacattatt cccagtataa taagcataac cgttaaaagc
      421 agtaagggat tgcgtatcat agaatgatgt agaaacaatt tcattgcaat gacggttatc
      481 ccgcttaaga tactgataaa aagcagtatc ataccaatgc caccaaatat ataattaggt
      541 ttggtggaat atgaacccat gaatttaaca gtggcaagat ctattaagac cttgaacgta
      601 cgctttatac catattttga ttttccaaat ttgcgtggat ggtgatgtac aggcacctca
      661 acaatagatg ctccaatcca tgatgcatga accggtataa agcggtgcat ctcaccatac
      721 agattgacct gccgaattat ctcaccgcga tatgctttga gtgaacatcc cagatcatgc
      781 agatatacac cggttacttt tgcaataagc cagttggcaa tgcgtgatgg gattttccgt
      841 gacaggagct tatcctgacg gtttttccgc cagccgctaa ccacatcata accttcttca
      901 attttctgca ccaatagtgg tatatcatta gcatcatttt gtaaatcaga atccataaaa
      961 atgataatat caccttttga ataatcaatg ccggctgcca tggctgctgt ctgaccaaaa
     1021 ttgcgcctga actgtaccac atgcacatgt ttatcttttt ttgcaatgga ttcaagaagt
     1081 ttgaaggaat tatcagtgct gccatcgtca acaaaaatta tctcataggt gtatgatgtt
     1141 ttttgcatag cttcaacaat gtgctgatat tgtggaacaa tgttttcttc ttcattataa
     1201 acaggtaata taatagaaag atatggttta tttgcacggt gcgctttcat accagcttct
     1261 ccagaatgta aggtttgttt tttagcctga atatcactat aatgaactat cgcaataaaa
     1321 ttattctttt gacaagaaaa ttattataaa aataatgtgg ttttaatgag gcgatagtaa
     1381 ccatggacaa gtcaaccgtg attgaaacaa tacagcaaga gcttgataac ctttttgccc
     1441 gctttagtga aataaagcaa ggtggcaaaa agattatttt tacccagtgc gatagtgtgc
     1501 cgtatgaagt ttttgtagcc catggattta ttgtgagcaa attgccacgc tgggtgatga
     1561 ctgcattatg taaacccgac agctcaatgt ataaaagagc agtggagtat tgcagccttg
     1621 ccgatgtgct cattgtgcca cagtattgta ctatcatacc ccgtgaagtg ttacagcaat
     1681 tgaccgtaca tgccatccag cattatgaag gctttgccca agatgcagtg gttaaccttc
     1741 acacaatact gtatttagtg ttgcagtttt taggagtaga gcaggaatac cctgatgatg
     1801 atagtctcaa gcaggcagtt gcagtgtatg aagaaatacg caaaataatg cgggcactga
     1861 ccaccatgta tgcggctgta ttcacccaga gtcagttgca attactgtgc gatgcagcat
     1921 tttcccttgt gccgcagcag agcttgcagt tgttgcagca gttataccac gcagttgagt
     1981 catcaactca tagtgacaat gaaccgactc aaaaggcgct tgtctacagt gactttagca
     2041 attgggatat atatgatgca tgtaatgaat ttgatattgt cattgtcgag gatgatagct
     2101 gcaatgggag gcggcagttt gacatttcat gccatacttc atcacaaaac ctgtactatg
     2161 agctattgta tgcttactca tttaaatcat ggtgccctgg catgcgtacg gtgcatgaac
     2221 gctgtgagct tatttacaag gcattgccaa actatgatat aaagcttgtt atttttatac
     2281 agtcttttct gaactcacgt aatgagcacc tgcaaagctt gtatgaacag tgtttactgc
     2341 agggtacgga tgctgtaata ctgcaggcag atgctattac aaggcagctt tcacattata
     2401 g
//
LOCUS       DAOSZP010000360         2393 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_34041_length_2393_cov_3.168948, whole genome shotgun sequence.
ACCESSION   DAOSZP010000360 DAOSZP010000000
VERSION     DAOSZP010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34041_length_2393_cov_3.168948"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..625
                     /locus_tag="PK348_07990"
     CDS             <1..625
                     /locus_tag="PK348_07990"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013323560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXK66192.1"
                     /translation="HPKFRPWLIEEAKKNGLIYRDQAFIPGKKGEYPEHLETYRTTKT
                     GIKLKLRPVKISDEELLKDFFYSLSDQSLYRRFISTRKDMPHERLQEMVVIDYTKEMV
                     ILAVIEQNEKEVVVGVGQYGIQEFQHWAEVAFAVRDDYQYKGIGQVLLSYLTQIAKRN
                     GLLGFTAEVLVENKPMLHLFEKMGFNIEKRSSAGVYELKMEFRGDEQ"
     gene            622..1983
                     /locus_tag="PK348_07995"
                     /pseudo
     CDS             622..1983
                     /locus_tag="PK348_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015858816.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
     gene            2113..>2393
                     /locus_tag="PK348_08000"
     CDS             2113..>2393
                     /locus_tag="PK348_08000"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HXK66193.1"
                     /translation="MKASGVMIPGAGIGKSIDECVRLAEQIGYPVVMKVVSRDILHKS
                     DAGGVALDILNKQEVMDAYEAIMKNCRAYKEDAVIDGIEVCEMVQKGVEL"
BASE COUNT          745 a          437 c          583 g          628 t
ORIGIN      
        1 tcatcccaaa ttcaggccat ggcttataga agaagccaag aaaaatggtc tcatctatcg
       61 tgaccaggct ttcataccgg gtaaaaaagg cgagtacccg gaacaccttg aaacataccg
      121 taccacaaag acaggcatca agcttaaact acgtcctgtc aagatcagcg atgaagaact
      181 cttaaaggac tttttctatt cactatccga ccagagcctc tacaggcgct ttatatcaac
      241 acgcaaagac atgccccatg agcggcttca ggagatggtg gtaattgatt acaccaaaga
      301 gatggtgatt ttagctgtga ttgaacaaaa tgaaaaagaa gttgtggtcg gcgttggtca
      361 atacggtata caggaatttc aacactgggc agaagtggca tttgctgtgc gtgatgatta
      421 tcaatataaa ggcattggtc aggtactgtt atcatatctg actcagatag caaagagaaa
      481 tggtttgctg ggattcactg cagaagtgct tgtggaaaac aagcccatgc tgcatctatt
      541 tgaaaaaatg gggtttaata tagaaaaaag gagtagcgca ggagtatatg aattgaagat
      601 ggaatttagg ggagatgaac aatgaaagca aaaaaggata gtattgtttc tttattccag
      661 cccaaaagtg ttgcagtagt tggtgcttca tcccaaccgg gcaaaattgg gttcagcatc
      721 acccgaaata tcattcaggg tggttttaag ggtaaaattt atccaataaa ccccaaaggt
      781 ggtgccattt taggcgttga ttgctaccca agcatttcag atgttaagga tgaaattgac
      841 gtagcaacaa tctgtgttcc tgcaaagtta gcatttgatg cagttaagga atgcgctgat
      901 aaaggcgtaa aacatgttca gattattacc tcaggcttct ccgaagttgg cgaggttgaa
      961 ctggaacaca aaattgtgga atatgccaat tccaaaggaa cacgggtact tggcccaaac
     1021 atctttggcg tatactctgc atcaggtaat tataattcaa cattctctgc cacagatatt
     1081 ttacgaggca atgttgccat ccttacccag agtggtgcac tggggatagc aatgataggg
     1141 aaaactgctg ttgaaaacat gggattatcc gctatcgtat cactgggtaa caaagcagat
     1201 attgacgaag cagactgttt agattttgtc atgcgcgatc aggaaacaaa ggttattttg
     1261 atgtacattg aaggcgtaaa agatggcgaa cgttttattg aagctgttcg cgaagcaaca
     1321 aagttaaaac caattgttgt attaaaatca ggccgatcaa aacgaggtgc aatggctgct
     1381 gctagccata ctggttcgct ggctggttca gatgaaattt ttgatgctat tgcacgacag
     1441 acagggttac tgcgtgctga aagcttacag gaagcattta actggtgtaa gtttttagca
     1501 tattcaccac gccccaaagg aaagaatact gttattgtta ccaatggtgg cggtattggt
     1561 gttatggcaa ctgatgcatg tgaaaaatat ggtgttgaac tgtatgacga ccagctagta
     1621 ttaaaagaag tatttggtcc ggcaacacct gactttggtt caaccaaaaa tccggtagac
     1681 attaccggtg gagcaaaagc tgcagattat gacctggcac tcaccgcacc aacacgttct
     1741 gaagacatgc acgcaactct ggcattgtac tgtgaaacag ctacatttga tgctgaaaac
     1801 ttagcaccaa tgatcagaga tacctatggc aagcatttgg ccagcggcaa gcctgttgcg
     1861 tatgcgatag ttggtggcaa agtagttgaa gacgcagtag ttcaactgcg taaaatgaat
     1921 gttccggttt tccctgaagt atacgaagct gtatcatgta tgggtgcgtt gtataaatac
     1981 accaacatgt tagccgaacg tgatgacagt gtagaagatg cacagatgga catagctact
     2041 attaacaaga ttattgatgg cgcacttgct gacggaagaa cattccttct tgcaaacgaa
     2101 gggcaggctg taatgaaagc ttccggcgtc atgataccag gcgcaggtat tggtaaatcg
     2161 atagacgagt gtgtacgatt ggctgagcag ataggttacc cggttgttat gaaggttgtt
     2221 tctcgtgaca tcttacacaa atctgatgct ggcggcgttg cattagacat attgaataaa
     2281 caggaagtaa tggatgccta tgaagctatt atgaagaact gtcgcgcata taaggaagat
     2341 gcagtcatag atggcattga agtatgcgaa atggttcaga aaggtgttga act
//
LOCUS       DAOSZP010000361         2385 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_34197_length_2385_cov_3.397854, whole genome shotgun sequence.
ACCESSION   DAOSZP010000361 DAOSZP010000000
VERSION     DAOSZP010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2385
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34197_length_2385_cov_3.397854"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1307
                     /gene="ftsH"
                     /locus_tag="PK348_08005"
     CDS             <1..1307
                     /gene="ftsH"
                     /locus_tag="PK348_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HXK66194.1"
                     /translation="LARAVAGEAGVPFFSISGSDFVEMFVGVGASRVRDLFDQGKRNA
                     PCIIFIDEIDAVGRLRGAGLGGGHDEREQTLNQLLVEMDGFETNEGVIIVAATNRPDV
                     LDPALLRPGRFDRQVVVDIPDIKGREKILAVHTRKIPLAKDVDLKVIARGTPGFTGAD
                     LANLVNEAALLAARRNKKRVSMLEMEDAKDKVLMGPERKSVLISAEEKKVIAYHEAGH
                     ALLGILTGADPVHKVTVIPRGRALGLTMHLPKEERHLHNREYWLNQITILFGGHVAEK
                     IKFGQVTTGSSNDIERATDIARRMVCEWGMSETLGPLAFGKKTEQIFLAREIAQHRDY
                     SEETAQLIDKEVHDIVTSCRDRAIKLIEENSDKFELLATNLIERETLNAQEIELLMKG
                     EPLPPIHNGENPQQPDEEPKIEKIKKKASQKPLLDDKEVIPT"
     gene            1324..>2385
                     /locus_tag="PK348_08010"
     CDS             1324..>2385
                     /locus_tag="PK348_08010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66195.1"
                     /translation="MIDDELDRLLQDIDSDKDYTAQKAHAKHFKEHVLDSELDETIHD
                     IPLPEKKNISEVVKFFKRGLSSGIHRHIELAKLLRSRLDITEVPEQIVPVIKKSSYSI
                     FKTIEDIEILTDKFIGEINEANLRRKATKDFPLYFPDDYPDDNRLDFIVLKEIYLLLR
                     SFNSIVKKDWTELEKQSAVFNFAEGGKQLSSVLNDIIVEGNSLCKATDVLLELIANHL
                     GLLSHHYNLSDADIRSKLQFNEFKSYTVQSLLNELDTSEFVQDQSEALKHNIHKTSTL
                     TSPDGMEEASDSNEYNKQKQYEISKSKTQSEYSTQQSTVPFTVKGTKPWNTREPYILS
                     VDENVLKIEQEDLEYMVYYT"
BASE COUNT          775 a          408 c          537 g          665 t
ORIGIN      
        1 tacttgcacg ggctgttgcc ggtgaggctg gtgtgccatt tttttccatc agtggttctg
       61 attttgtgga aatgtttgtt ggggttggtg catcgcgggt acgtgacctg tttgatcagg
      121 gaaagcgtaa tgctccctgc atcatattta ttgatgagat agatgctgtg ggaaggcttc
      181 gtggtgctgg actgggtggt ggccatgacg aacgtgaaca gacgttgaat cagcttcttg
      241 ttgagatgga tggatttgaa actaacgaag gtgttattat tgttgctgct accaacaggc
      301 ctgatgtact tgaccctgcg ttgctcaggc ccggccgttt tgaccgtcag gttgtcgttg
      361 acatacctga tatcaaggga agggaaaaga tattagctgt tcatacccgc aaaatccctt
      421 tggcaaagga tgtagactta aaagttattg cccgtggtac acctggattt actggtgcag
      481 atctggcaaa tcttgttaat gaggctgcac tgcttgctgc acggcgcaat aaaaagcgcg
      541 tgagcatgct cgagatggag gatgccaagg acaaagtatt gatgggtcct gaaagaaagt
      601 cagtcctcat ttctgccgaa gaaaaaaagg tcattgccta tcatgaagct gggcatgcat
      661 tgctgggtat acttaccgga gccgaccctg tgcacaaagt gactgttatt cctcgtggaa
      721 gggcgttagg cttaaccatg catttgccca aagaagaacg ccacttgcat aatagagaat
      781 actggctcaa tcagatcacc attttatttg gtggccacgt tgctgaaaag ataaaatttg
      841 ggcaggttac aacaggttca agcaatgata ttgagcgtgc tactgatatt gcccgacgta
      901 tggtgtgtga atggggaatg agtgaaacac tgggcccgtt agcatttggc aaaaaaacag
      961 aacagatttt tctggctcgt gagatagctc agcacagaga ttacagcgaa gaaacagcac
     1021 agcttattga taaagaagtt catgatatcg tcaccagttg cagggacagg gctataaaac
     1081 tgatagaaga aaacagtgat aaatttgaat tgcttgcaac aaatcttata gaacgtgaaa
     1141 ctctgaatgc tcaggaaatt gaactattaa tgaaaggtga accattaccg cctattcata
     1201 atggcgaaaa tccacaacag cctgacgaag agcctaaaat agaaaaaata aaaaagaaag
     1261 catcgcaaaa acctttgctg gatgataaag aagtgattcc aacataaagt gtttggggtt
     1321 tgtatgatag atgatgaact ggacaggtta ttgcaggata ttgatagtga taaggattat
     1381 acagctcaga aagcacatgc aaagcatttt aaagaacatg tccttgatag tgaactagat
     1441 gaaacaatac atgatatacc attacctgag aaaaaaaata tatcagaagt ggtaaaattt
     1501 tttaagcgcg ggctgtccag tgggatacac aggcatattg aattggcaaa gcttttacgg
     1561 tcacgtttgg atataacgga ggttcctgaa cagattgttc cggtaataaa gaaatcgtct
     1621 tactccattt ttaaaactat tgaagatatt gaaattctta cggataaatt tattggtgaa
     1681 ataaatgaag caaatttaag aagaaaggca accaaagatt ttccccttta tttccccgat
     1741 gattatcctg acgacaaccg gcttgacttt atagtactta aagaaatata tttgttattg
     1801 cgttcattta atagtattgt taaaaaagat tggacagagc ttgaaaagca atctgcagtg
     1861 tttaactttg ctgagggcgg caaacagctt tcatcagtac ttaatgatat tattgttgaa
     1921 ggaaacagcc tgtgtaaagc tacagatgtg ttactggaat taattgctaa tcatttaggg
     1981 ttactttctc atcattataa tctgtctgat gctgatatac gttccaaatt gcaatttaat
     2041 gaatttaaat catatacagt gcaaagtttg ttaaatgaac tggacacctc tgaattcgta
     2101 caagatcaat cagaagcttt aaagcataat atccacaaaa catcaacgtt aacttctcct
     2161 gatggtatgg aagaagcttc cgattccaat gaatataata aacaaaaaca atatgaaata
     2221 agcaaaagta aaactcaatc tgaatacagt acccagcaaa gtacagttcc atttacagtt
     2281 aaaggcacaa agccatggaa tactcgtgaa ccatatattc tatcggttga tgaaaacgta
     2341 ttgaagatag aacaggaaga tcttgagtat atggtctatt atacc
//
LOCUS       DAOSZP010000362         2379 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_34312_length_2379_cov_3.340792, whole genome shotgun sequence.
ACCESSION   DAOSZP010000362 DAOSZP010000000
VERSION     DAOSZP010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2379)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2379)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2379
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34312_length_2379_cov_3.340792"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="PK348_08015"
     CDS             <1..343
                     /locus_tag="PK348_08015"
                     /inference="COORDINATES: protein motif:HMM:NF017555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DALR anticodon-binding domain-containing
                     protein"
                     /protein_id="HXK66196.1"
                     /translation="ICSIFREAQNRGIEYKKIITNTEYFNNQETIALLKLMARFPEEV
                     YDAASSFEPHRITVYCMRLAQAYHKFYTEHRVLTDNEDRTQSYLTLCDGVRIILKNAL
                     ALLGVSAPERM"
     assembly_gap    560..569
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <570..1646
                     /locus_tag="PK348_08020"
     CDS             <570..1646
                     /locus_tag="PK348_08020"
                     /inference="COORDINATES: protein motif:HMM:NF014517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidohydrolase family
                     protein"
                     /protein_id="HXK66197.1"
                     /translation="TLRLAIEHALHSADIIIISGGLGPTVDDITFETLEKMFALKPVI
                     HEPSKERMEYFFNAIGKSVLPSDFKMVTVPENAVVFVNDAGLAPGFAITVKEKDIIVL
                     PGVPRELHSMIGKVIDYLESHYQLKKKNSVTFKVVMMREAEVDSGVKQIVNSIKGVDV
                     SITYEAGMTTVMLTANDESILPVDSIVDKAKELFGSKMVYGYNSLEEKVVKVFASHGK
                     TIAIAESCTGGLIAKRITDIPGSSNVLLGGIVAYSNQSKISMLGVNPDTLQTYGAVST
                     ETALEMAAGAKEKFNADYGISTTGIAGPDGGTKDKPVGLVCFGIAGSNTFTTQIQFAG
                     NRERIRTMATNYILTRLLTCAGLE"
     gene            1643..>2379
                     /locus_tag="PK348_08025"
     CDS             1643..>2379
                     /locus_tag="PK348_08025"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXK66198.1"
                     /translation="MKRVFILFTILLFFNAASYAIESSQLFEQGMEAFRMGNYASSEL
                     IFRKVIDANDSFKDRAWYYLALSIFYQKQYRSAIFELNRFLLLCTTPDLCSEARYWIA
                     ESYYYLNDYIKAIEEYNRFISQSKNKQLIINSYDRIGEIYFNQKRYDEAIIEWKKALN
                     ISSKKYEDSNRVIKIGEALFLSERYNEALDLLEPLIYATGDNTIQSKARLLVGRIYQL
                     TGKHWQAIKYFNEIPESMLRVPSFISV"
BASE COUNT          782 a          353 c          496 g          738 t
ORIGIN      
        1 aatctgttca atattcagag aggctcagaa cagaggtatt gagtacaaaa aaattattac
       61 caatactgag tattttaata atcaggaaac aattgcttta ttaaaactta tggcacggtt
      121 cccggaagaa gtatatgatg ctgcatcaag ctttgaacca caccgtatta ctgtttactg
      181 tatgcgctta gcacaggcat accataaatt ttatactgag cacagggtat taaccgataa
      241 tgaagaccgt acccagagct atctcacatt atgtgatggg gttcgtataa ttttgaaaaa
      301 tgctcttgca ttgcttggtg tgagtgcacc tgaaaggatg taaaaattgc attaaaaaaa
      361 ataaattatt gattttttat agctgcaatt gttgtattac agtaaatcat aaagtaccat
      421 ttaaagatat tccatgaaag cttcactaat agctacaggt agtgaactgg tttgtggccg
      481 tgttcatgaa agcaataatt tctatttaag tcagcacttg ctggcaattg gattgccagt
      541 gataatgcac tatgtagttn nnnnnnnnna ccttgcgtct ggcaattgag catgcattgc
      601 atagtgcaga tattattata atttctggtg ggttagggcc taccgttgat gatattacat
      661 ttgaaactct ggaaaaaatg tttgcactaa aacctgtgat tcacgagcct tcaaaagaac
      721 gcatggaata tttctttaat gctattggga aatcagtttt gccttctgat tttaagatgg
      781 tgactgttcc tgaaaatgca gttgtttttg ttaatgatgc tggacttgca ccgggttttg
      841 ctattactgt taaagaaaaa gatattatag tattgccagg ggttccgcgt gaattacatt
      901 ccatgattgg taaggttatt gattatctgg aaagtcatta tcaactgaaa aaaaagaact
      961 ctgtaacatt caaggttgtt atgatgcgtg aagctgaagt tgattcgggt gttaaacaga
     1021 tagttaattc aataaagggt gttgatgttt ccattacgta tgaagccggg atgactactg
     1081 taatgctcac tgctaacgat gaaagtatat tgccagttga tagtatagtt gataaagcaa
     1141 aagagctttt tggctcaaaa atggtatatg gatataattc attagaagag aaagttgtta
     1201 aggtattcgc cagtcacggt aaaacaatag ccattgcaga gtcatgtaca ggggggctta
     1261 ttgcgaaaag aattacagat ataccgggtt cttccaatgt gttattgggt ggtatagtgg
     1321 catacagcaa ccagtcaaag atatcgatgt taggggttaa tcctgatact ttgcaaacat
     1381 atggtgcggt aagtacggaa actgcattgg aaatggcagc aggtgctaaa gaaaaattta
     1441 atgccgatta tggaatttca acaacaggta tagccggtcc tgatggtggg accaaagata
     1501 aaccggtagg attggtatgt tttggcattg caggcagtaa tacttttact acacagatac
     1561 aatttgctgg taatcgtgaa cggatacgta caatggcaac taattacata ttaaccagac
     1621 tattaacgtg tgcaggactt gaatgaaaag agtatttata ctatttacta tattactttt
     1681 tttcaatgct gcttcgtatg caatagaaag ctcacagctt tttgagcagg gtatggaagc
     1741 attcaggatg gggaattatg cttcctcaga acttatattc agaaaagtaa ttgatgctaa
     1801 tgattctttt aaggacagag catggtatta tctggcctta tcaatatttt accaaaaaca
     1861 ataccgttcg gctatttttg agttaaacag atttttactt ttatgtacaa cgcctgatct
     1921 ttgtagtgaa gcgcggtact ggattgcaga atcatattat tacctgaatg attatatcaa
     1981 ggctattgaa gaatataaca ggttcatttc tcagagcaaa aataaacagc tgataataaa
     2041 ttcttatgac cggataggtg aaatatattt caatcagaaa cgctatgatg aagcaattat
     2101 tgaatggaaa aaagcattaa atataagcag taaaaaatat gaagacagca atagagttat
     2161 aaaaattgga gaagcgttgt tcttaagcga acgttataat gaagctctgg acttattaga
     2221 gcctttgatt tatgctacag gtgataatac cattcaatca aaagccaggc tactggttgg
     2281 aaggatatat cagcttactg gcaaacactg gcaggcaata aaatatttca atgaaatacc
     2341 tgagtcaatg ttacgagtgc catcttttat aagtgtaca
//
LOCUS       DAOSZP010000363         2377 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_34350_length_2377_cov_2.996124, whole genome shotgun sequence.
ACCESSION   DAOSZP010000363 DAOSZP010000000
VERSION     DAOSZP010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2377
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34350_length_2377_cov_2.996124"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..898
                     /gene="hemA"
                     /locus_tag="PK348_08030"
     CDS             <1..898
                     /gene="hemA"
                     /locus_tag="PK348_08030"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HXK66199.1"
                     /translation="WATQYETTGTILNKLFHQAFRTAKRVRTETEIARNPLSIAFIAV
                     ELAKKIFEDLSKQSVLLIGAGEMAELILKYLTKFKIGSVTLANRSLHNAQRIASEINQ
                     DATIITLAEIMESIPNVDIIISSTGAHHYMLTKDMIQEAMKKRTQPVFLIDIAVPRNI
                     DPEAGNIHDVFLYNIDDLKSIADENLKSRLNETKLAYEMIDADTEEFLHWYESLQIVP
                     LIAKIKESFETIRNNELKKYRRRKLKHLNNEDFAIIEELTHQIMTKTLHIPITYLKEL
                     GANANSKEAKEKAKLLQEIFKL"
     gene            895..1950
                     /gene="ahbD"
                     /locus_tag="PK348_08035"
     CDS             895..1950
                     /gene="ahbD"
                     /locus_tag="PK348_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019241290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006785 - heme B biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme b synthase"
                     /protein_id="HXK66200.1"
                     /translation="MNGPRLIFWELTKRCNLRCVHCRAESYDREYINELSTQQIHNVI
                     DTIASNYRPIIVLTGGEPLYRKDLFDIAAYAHEKGLPIALATNGTLIDEQIAQQIKAS
                     GFQRVSISIDGPDAHTHDSFRGIPGSFDKALHGALLLYKAGVPFQFNTTITKRNVNSI
                     EAILKLAQEKKAAALHVFMLVPVGCGVEIADTDMLSKEQYEEVLRWFYYKTKEAGIEF
                     KATCAPHYYRIIRQEAKKEGRKLTFETDGLAAVTRGCLAGSGVCFISHRGDVQPCGYL
                     PLVAGNVLQTPFNQIWESSELFANLRNLSKLKGKCGLCEYKAFCAGCRARAYYATGDY
                     MDEEPYCIFEPKRMTHA"
     gene            1943..>2377
                     /locus_tag="PK348_08040"
     CDS             1943..>2377
                     /locus_tag="PK348_08040"
                     /inference="COORDINATES: protein motif:HMM:NF037131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HXK66201.1"
                     /translation="MLDTKQLLKTVQIQVPLVTRPFSAIADNLHSTEDEVIEALASLK
                     QKGILRSIAGIFNAKKLGYQSNLVAFVVPHNNIEEAVRVINPLPGVSHNYLRDGDYNI
                     WFTLALPQEENFSDYVEELAKKAGAMRYNIFKATTMVKLSARF"
BASE COUNT          773 a          488 c          494 g          622 t
ORIGIN      
        1 ctgggctaca cagtatgaaa ctacaggcac tatcttaaat aagctgtttc accaggcttt
       61 cagaacggca aaacgtgtac gcaccgaaac tgagattgcc cgcaatcctt tatccatagc
      121 atttattgcg gttgaacttg caaagaagat atttgaagac ctttcaaaac aatctgtcct
      181 cttaattggc gctggcgaga tggccgaact tattttaaaa tatttaacta aatttaagat
      241 tggctcggta acgttagcta accgttcatt acacaacgca cagcgaattg ccagtgaaat
      301 caatcaggat gcaactatca tcacactggc agaaattatg gaatctatac ctaatgttga
      361 tataataata tcctcaacag gggcgcacca ttatatgctg acaaaggata tgatacagga
      421 agcaatgaaa aagcgtacgc aaccggtatt tttaattgat attgctgttc cccgcaatat
      481 tgaccctgaa gcaggcaaca tccatgatgt gtttttatac aacattgatg atttaaagtc
      541 aatagctgac gaaaatttaa aaagcagact taacgaaaca aagttagcat atgaaatgat
      601 agatgctgac actgaagaat tccttcactg gtatgaaagc ctgcagatag tgccattgat
      661 tgctaaaatt aaagaatcct ttgaaaccat ccgcaataac gaactaaaaa aataccgccg
      721 ccgaaaactc aaacatttaa acaatgaaga ttttgctatc attgaagaac ttacccatca
      781 gatcatgact aaaacgcttc atatacccat tacgtatcta aaagagcttg gcgcaaacgc
      841 taactcaaaa gaagcaaagg aaaaagctaa attacttcag gagatattca agctatgaat
      901 ggccctcgcc tcatcttctg ggaacttaca aaacgctgca atctacgctg tgtgcactgc
      961 agagctgaaa gctacgacag ggaatatatc aatgagcttt caacacagca gatccataac
     1021 gtcattgata ctattgccag taactatcgc cctattattg tattaacggg cggcgaacca
     1081 ctgtatcgta aagatttatt tgatattgcc gcgtatgcgc atgagaaagg tttgccaata
     1141 gcacttgcaa ccaatggcac actgatagat gaacagattg cacagcaaat aaaggcaagc
     1201 ggatttcaac gggtaagtat ttccattgac gggcctgacg cacacacaca cgattcattc
     1261 agaggaattc ctggttcatt tgataaagcc ttacatggtg ctttactgct ctataaagct
     1321 ggtgtgccgt ttcagttcaa cactacaatt acgaagcgaa acgtaaacag tattgaagcc
     1381 attttaaaac tggcacaaga aaaaaaagct gctgcactgc atgtgtttat gctggtgcct
     1441 gttggctgcg gcgttgagat agcggatact gatatgcttt caaaagaaca gtatgaagaa
     1501 gtgctccgct ggttttacta caaaactaaa gaagctggca ttgaattcaa ggcaacgtgt
     1561 gcaccccact actatcgcat catacggcag gaagcaaaaa aggaaggacg taaactcacc
     1621 tttgaaaccg acggacttgc cgcagtgacg cgcgggtgtc tggctggaag tggtgtgtgt
     1681 tttatttcgc acaggggcga tgtacagcca tgtgggtatc tgccacttgt tgcaggcaat
     1741 gtactgcaaa caccgtttaa tcaaatatgg gaatcatcag agctgtttgc caatctgcgc
     1801 aatttgtcaa aacttaaagg caaatgcggc ttatgcgaat ataaagcctt ttgtgcgggc
     1861 tgccgcgcac gggcatatta tgcaactggt gattacatgg atgaagaacc gtactgcata
     1921 tttgagccaa agaggatgac ccatgcttga cacaaaacaa ctgcttaaaa cagttcagat
     1981 acaggtaccg ttagttacaa ggccatttag cgccattgct gataatcttc attcaactga
     2041 agatgaagtt attgaagcgc ttgccagttt aaaacaaaag ggcatactga gaagtattgc
     2101 tggaatattc aatgcaaaga aacttggcta tcagtcaaac ctggttgctt ttgttgtgcc
     2161 acacaacaat attgaagaag cagtacgtgt tattaaccct ttaccaggtg taagccacaa
     2221 ttatcttcgt gatggtgatt acaatatatg gttcacattg gcgctgccac aggaagaaaa
     2281 tttttctgat tatgttgaag agcttgcaaa gaaagcgggt gccatgcgtt ataatatttt
     2341 caaagcaaca accatggtga aactttctgc ccgcttt
//
LOCUS       DAOSZP010000364         2367 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_34526_length_2367_cov_2.950692, whole genome shotgun sequence.
ACCESSION   DAOSZP010000364 DAOSZP010000000
VERSION     DAOSZP010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2367
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34526_length_2367_cov_2.950692"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /locus_tag="PK348_08045"
     CDS             <1..233
                     /locus_tag="PK348_08045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66202.1"
                     /translation="RETIDRKNKKMKELIEYIKKLHLLLAYYKLSPEDFNKIDFTKAY
                     IPAPEMPAPPPSIEVKEKTIEYVPVREEIIQD"
     gene            551..1975
                     /gene="cysS"
                     /locus_tag="PK348_08050"
     CDS             551..1975
                     /gene="cysS"
                     /locus_tag="PK348_08050"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000570133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HXK66203.1"
                     /translation="MALKIFNTLTRRLEPFIPGGVPKENIEDYPPVTIYSCGPTVYSY
                     AHIGNFRTFVFNDFLRRYLKFRGFKVNHAMNITDVDDKTIAGALNDGITLREYTYKYT
                     NIFFEDLRTLNIEPVEHYPRATESIEAMIDIIAHLDKKGLIYEKDGSIYFSIAKFHRY
                     GRLSNVTSMDIKAGARYDADEYSKEDVRDFALWKAPKENEPYWDTPFGKGRPGWHIEC
                     SAMVRKIFGTTIDIHTGGVDLIFPHHENEIAQSEAAYDEPFVRYWIHVEHLLVEGSKM
                     SKSLGNFYTLRDLLDKGYSPRAIRYLLLTAHYRKQLNFTFDGLNQAAQALLRIDNLLA
                     RLNDIKHDALPNQKVTNRCNAFITAFTETVDDDLNIAGGTGIFFDFIHDINTMIDSNS
                     LCKSDAMYVMDVLKKIDTVFGFIFFPEVLHLNKEEIEALIEERNKARKEKNFAKADAI
                     RDELLQKGIILQDTKEGTRWIRKS"
     gene            1957..>2367
                     /locus_tag="PK348_08055"
     CDS             1957..>2367
                     /locus_tag="PK348_08055"
                     /inference="COORDINATES: protein motif:HMM:NF019644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase substrate-binding
                     domain-containing protein"
                     /protein_id="HXK66204.1"
                     /translation="MDTQIVVGRKVVLEYLTTLQGDATLYVSESAHGRIIDVIIAAAH
                     SKHIPVQKVNKSWFDSHCAAHHQGVALVMNYRPKKEEKQCELKKIASSHGVIVACDEI
                     TDPHNIGSIIRTTEALGGSAVVVTKAHAPEITPTI"
BASE COUNT          786 a          478 c          452 g          651 t
ORIGIN      
        1 ctcgtgaaac aattgaccgt aaaaataaaa agatgaaaga acttattgaa tatataaaaa
       61 agcttcacct ccttttagcc tattataagc tctcacctga ggattttaat aaaatagatt
      121 ttacaaaagc atatatcccg gcaccggaaa tgcctgctcc tcctccttcc attgaggtaa
      181 aagaaaaaac cattgagtat gtacctgtca gagaagaaat tatacaggat tgacatatgt
      241 atatagaaaa aatgtattaa taactcctga caaaaaataa aaggggctgt ccccaaaaaa
      301 aagaaaatat atcaaaatcc gaagtaataa aaagagcact tacactgttt tttgaacatt
      361 ataaaataaa caatccgtat gaaaaaggca aagatctttt tggtaaatat gggagtggcc
      421 agagtaattt atccacctgg caaagagcat atacatattt taatgctgca ctttttcact
      481 tttacttgac tgtttacagt agctgcaatt tctttgttag tactatcacc aaacttctgg
      541 aggaaaaacg gtggcactta aaatattcaa tacactgacc cgacgtcttg aaccattcat
      601 acctggtggt gtacccaaag aaaatataga agattatccg ccagttacca tatattcgtg
      661 cggccccact gtgtatagct atgcccatat aggtaacttc cgaacatttg tgtttaatga
      721 ctttttgcgg cgctatctta aattcagggg atttaaggtt aatcacgcaa tgaatatcac
      781 cgacgttgat gataagacaa tagcaggtgc tcttaatgat ggcataacac tcagggaata
      841 tacttacaaa tacacaaata tattctttga ggatcttaga acacttaaca ttgaacctgt
      901 ggaacattac cctcgtgcta ccgaatccat tgaggccatg attgacatca ttgcccattt
      961 agataagaaa gggcttatct atgaaaaaga tggttccatc tatttcagta ttgcaaaatt
     1021 ccaccggtat gggcgtttaa gcaatgttac cagtatggat attaaagctg gtgcccgcta
     1081 tgacgctgat gaatacagca aagaggatgt gcgcgacttt gcactgtgga aagcaccaaa
     1141 agaaaacgaa ccatactggg ataccccatt tggcaaagga aggcctggat ggcatattga
     1201 atgttcagca atggtgcgta aaatctttgg caccaccatt gatattcaca caggaggcgt
     1261 tgatttaatc ttccctcacc atgaaaatga gatagctcaa agtgaagctg cctacgatga
     1321 accatttgtg cggtactgga tccatgttga acaccttctt gttgaaggtt caaagatgtc
     1381 aaaatcactg ggcaattttt atacactgcg cgaccttctt gataaagggt atagtcctcg
     1441 ggcaatccgc tacctgcttt taactgctca ttaccgcaaa caactcaatt ttacctttga
     1501 cggattgaat caggctgctc aggcacttct tcgcattgat aaccttttag cacgtctcaa
     1561 tgacataaaa catgatgcat tacccaatca aaaagttacc aaccgatgca atgctttcat
     1621 aactgcgttt accgaaacag ttgatgatga tttaaacatt gcaggaggca ccggcatctt
     1681 ttttgatttc atccatgata ttaataccat gattgattcc aattcattat gcaaatcaga
     1741 tgcaatgtat gtaatggatg tgttaaagaa aatagataca gtatttggat ttatcttctt
     1801 ccctgaagtt ttgcatctca ataaggaaga aattgaagcc ctgattgaag aacgtaacaa
     1861 agctcgcaaa gaaaaaaatt ttgcaaaagc tgatgcaatc cgtgatgagc ttctgcaaaa
     1921 aggcattata ttacaggaca ccaaagaagg tacccggtgg atacgcaaat cgtagttggc
     1981 cgcaaggtag tgcttgaata ccttacaacc cttcaagggg atgcaacact gtatgtatcc
     2041 gaatctgcac acgggagaat catcgatgtt attattgctg cggcccacag taaacatatc
     2101 cctgtacaga aagtaaacaa aagctggttt gattcacact gtgctgccca tcatcagggt
     2161 gtagctcttg ttatgaacta tcgccccaaa aaggaagaaa aacaatgtga gttaaaaaaa
     2221 attgcttcca gtcacggtgt tattgtggcc tgtgatgaga taaccgatcc acacaacatt
     2281 ggctccatca tccgaaccac cgaggccctt ggtggcagtg cagtagttgt tacgaaagca
     2341 catgcacctg agattacacc aaccatc
//
LOCUS       DAOSZP010000365         2358 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_34683_length_2358_cov_3.877985, whole genome shotgun sequence.
ACCESSION   DAOSZP010000365 DAOSZP010000000
VERSION     DAOSZP010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2358
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34683_length_2358_cov_3.877985"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..593
                     /locus_tag="PK348_08060"
     CDS             <1..593
                     /locus_tag="PK348_08060"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF013296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HXK66205.1"
                     /translation="KLGQCPDLIAINNGTTHGNVRGNIDLKLTYNTYKAMAPWGVGIA
                     QHGTTGTAIEDVGKFYKYGIFKANVGTTWQNVIWGIDTDEYGVAMEKGPSYQKNPDKG
                     ISMDLWKKMEEYALSNNMKGGNMKKLFKPFKDAMAQEYHSNAKIRERIDQAVYETAAA
                     YFKALQAVGKADDVKAQMEKTVTKQEVKESKERDYE"
     gene            609..2195
                     /locus_tag="PK348_08065"
     CDS             609..2195
                     /locus_tag="PK348_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004785085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HXK66206.1"
                     /translation="MKKVWWKHGVIYQIYPRSFKDSNHDGIGDINGITECLDYLQDLG
                     IDGIWLSPIYRSPMYDFGYDVSDYCDIDPVFGTMRDFKHLVKEAEKRNIAIIMDMVFN
                     HTSHLHPWFLESRSSKDNPKRDWYIWHPGKKRKVPNNWMAAFGGRAWEWDPLTQEYYL
                     HLFTKQQPDLNWRNPNVKKAMFDVLRFWMDNGVKGFRFDVINYIMKDPLFRNNPYKFR
                     LTYPRRHDLQYHLYDRNQPEIHDILKELRHLLDSYGQIMSVGETYPNEGEREPLMAAS
                     YLGNNDELHLSFDFSTIYISFSAKEFTSILIQWYNAIGNKGWPSHVLSNHDQSRAITR
                     IAKNDLQKAKLLAVLLLTQKGTPFIYYGEEIGMVDGKIPYRYIQDPVGKKYWPFHTGR
                     DKARTPMQWDATSYAGFSTAKPWLPVNPDYTRTNVALQSKNAESMMQFYRQLIALRKS
                     RKELYAGEIEFLPGSDDVMLYKRVWNNDTCLVALNFSSSKRKINFDSTCRVLLSSYQK
                     NIIDVQTITLDPFEAVICSK"
     gene            complement(2211..>2358)
                     /locus_tag="PK348_08070"
     CDS             complement(2211..>2358)
                     /locus_tag="PK348_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011554193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HXK66207.1"
                     /translation="SAVVIHGHGTGSLKKAVRDYVKHSFYAKNFRPGEPEEGGDGVTI
                     IQLR"
BASE COUNT          785 a          420 c          492 g          661 t
ORIGIN      
        1 caaagttagg gcaatgtcct gatttaattg ccattaataa tggaaccacg catggcaatg
       61 tgcggggaaa tattgatctc aagctaacct ataataccta taaggcaatg gcaccatggg
      121 gtgtgggaat tgcgcagcac gggacaacgg gaacagctat agaggatgtt gggaaatttt
      181 ataagtatgg aatattcaaa gctaatgttg gtacaacctg gcagaatgta atatggggta
      241 ttgatactga tgaatatggt gttgcaatgg aaaagggccc ttcatatcaa aaaaatcccg
      301 ataaaggcat ttcaatggat ttatggaaaa agatggaaga gtatgcacta tccaataaca
      361 tgaagggtgg taacatgaaa aagttattta agcccttcaa ggatgctatg gcacaggaat
      421 accattcaaa tgcaaaaata cgtgaaagga ttgaccaggc agtatatgaa accgctgctg
      481 catactttaa ggcattacag gctgtgggaa aagctgatga tgtaaaagct cagatggaaa
      541 agactgtaac caaacaggaa gtcaaagaat caaaagaaag ggactacgaa taattaaaca
      601 ataaattcat gaagaaagtc tggtggaaac acggggtaat atatcaaatt tatccccgca
      661 gctttaaaga ttccaatcat gatggtattg gtgatataaa cggaattact gagtgccttg
      721 attacctgca ggatttaggt attgatggca tctggctatc gccaatttac cgttcaccaa
      781 tgtatgattt tggatatgat gtcagtgatt attgcgatat tgaccctgtt tttggtacca
      841 tgcgtgattt caaacacctt gttaaagagg cagaaaaaag aaatattgca ataattatgg
      901 atatggtttt taatcataca tcccatttgc atccatggtt tttagaatca cgctcatcaa
      961 aagataatcc taaaagagac tggtatatat ggcatccagg caaaaagaga aaagtgccca
     1021 ataactggat ggcagctttt ggcggaaggg catgggaatg ggacccatta actcaagaat
     1081 attaccttca tctttttacc aagcaacaac ctgatctaaa ctggcgcaat ccaaacgtga
     1141 aaaaagcaat gtttgatgtc ctgaggttct ggatggataa tggagtcaaa ggattcaggt
     1201 ttgatgtgat aaattatatc atgaaagatc cacttttcag aaacaatccg tataagttcc
     1261 gcctgacata tccacgacga catgacctgc aatatcacct ctatgataga aatcaacctg
     1321 agatacatga tattttaaaa gaattacgcc acttgttaga ttcttatggt cagattatga
     1381 gtgttggtga aacctatccc aatgagggtg aacgtgaacc actgatggct gcttcgtatt
     1441 tagggaataa cgatgaatta cacctttcat ttgatttttc tactatctat atatcgttta
     1501 gtgcaaaaga atttacatcc attcttatac agtggtataa tgctattggc aacaagggat
     1561 ggcccagcca tgttttgtca aatcatgatc agtcgcgtgc tattacacgt atagcaaaaa
     1621 atgacttgca aaaagctaaa cttcttgcgg ttttactgct tactcaaaag ggcacccctt
     1681 ttatatatta tggcgaagaa attggaatgg ttgatgggaa gatcccttat agatatattc
     1741 aggatcctgt aggcaaaaaa tactggcctt tccatacagg aagagataaa gcgcgcaccc
     1801 ccatgcagtg ggatgcaacc agctatgcag gcttttcaac cgctaaaccg tggctgccag
     1861 taaatcctga ctatacacgc actaatgtag cactacagtc aaaaaatgca gaatctatga
     1921 tgcaatttta ccggcaactg attgcattaa ggaaatctcg taaagaatta tatgctggtg
     1981 aaatagaatt tttacccgga tctgatgatg taatgcttta taaacgtgtg tggaacaatg
     2041 atacatgtct tgtagcatta aatttttctt cttcaaaaag aaaaatcaat tttgatagca
     2101 catgcagagt gttattatca tcgtatcaaa aaaacatcat tgatgtgcaa accatcacgc
     2161 tggatccttt tgaagcagtt atttgcagca agtgattatg ggcaatcctg ctatcgcaac
     2221 tgaatgatag taacgccgtc accgccttct tcaggctcgc cgggtcggaa atttttagca
     2281 tagaatgaat gttttacata atcccgtact gcttttttca atgatcctgt accatgtcca
     2341 tggataacaa ctgcagac
//
LOCUS       DAOSZP010000366         2358 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_34687_length_2358_cov_2.935736, whole genome shotgun sequence.
ACCESSION   DAOSZP010000366 DAOSZP010000000
VERSION     DAOSZP010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2358
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34687_length_2358_cov_2.935736"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..615
                     /locus_tag="PK348_08075"
     CDS             <1..615
                     /locus_tag="PK348_08075"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXK66208.1"
                     /translation="APEHLDHIFEPLYTTKEKGSGLGLAIVYAIVKRHKGFIDVMSTP
                     GTGTSFYIYLPVQPLHNVADETQNHALQAGTGTIIIIDDDDNICLLYQEALKLGGLQA
                     ITFTNPNEAFAYIDAHKDTIMLVVSDVRMPTVSGEDVLDYIKNNYPDIPVVLMSGYYD
                     DTMKEQLLRKGAYQVWQKMYDIHEIVHKVKGVLNNYARKNNIRS"
     gene            587..>2358
                     /locus_tag="PK348_08080"
     CDS             587..>2358
                     /locus_tag="PK348_08080"
                     /inference="COORDINATES: protein motif:HMM:NF013755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HXK66209.1"
                     /translation="MPEKTIFVVDGHALCYRAYFALQRNPLINSKGQNVSAIYGFFRM
                     LLRLLQTQKPDYTIIAFDPPKKSFRFSMYPEYKAQRAKMPDDLRSQIEEIKAILQEMG
                     FTVVIMDDYEADDVLGSIARQYSDSYKVVLVTGDKDAYQLLSDKNVSIYAGKKGITEF
                     ESIDADAVVEKLGVRPEQIIDYMSLTGDAVDNIPGVKGIGEKTASKLLQQYGTLDNIY
                     KHLHELTGKIKEQIESQKDSAYLSRQLVTIKTDIPLSLSIEKALTPDLHNEKVRQIFK
                     NLEMDSIARELGDTEKSISIKEIAGYSTVTDIDTLNNVIAAIKNAGLVSFDTETTSSR
                     PMEAELVGVSFSIAPQSGWFIPVGDSSLWGYNAVPKDQLLQKIKTILEDDTIKKVGQN
                     IKYDMLIMLNCGITLTGIYFDTMVASYLLNPGLRRHNLDDLAAEYLNYTKITYDELVK
                     DGKKVQHITQVPLQKLSHYAIEDADIALRLYHILYKKLEQENLLELFFNVEMPLVTVL
                     AYMEYYGVKIDLEHFETLSKELSHKIKQTEEKIYSLAGIPFNINSTRELSHILFEKIG
                     LKPVKKTKTGFSTDIQVLEALRGQ"
BASE COUNT          795 a          414 c          480 g          669 t
ORIGIN      
        1 gctcctgagc accttgacca tatttttgaa ccactgtata ctacaaaaga aaaaggaagt
       61 ggattgggat tggcaatagt atatgctatt gtaaagcggc acaagggttt tattgatgta
      121 atgtcaacac caggtacagg cacaagcttt tatatatatt tacctgttca accattacac
      181 aatgtggctg acgaaacaca aaaccatgcc ttgcaggcag gcactggtac cataattatt
      241 attgacgatg atgataatat ttgtctttta tatcaagaag cattgaaatt aggtggcttg
      301 caagcaataa catttactaa tcccaacgaa gcatttgcgt atattgatgc ccataaagat
      361 acaataatgc ttgttgtaag cgatgtacga atgccgacag tttccggtga agacgtcctt
      421 gattacataa aaaataatta tccagatata cccgttgtac tgatgagcgg ctattatgat
      481 gatacaatga aagagcaatt attgcgtaag ggtgcctacc aggtatggca aaagatgtat
      541 gatattcatg agatagtaca taaagtaaaa ggtgtgttaa ataattatgc cagaaaaaac
      601 aatattcgta gttgatggcc atgcattatg ctacagggca tattttgcat tgcaacggaa
      661 tcctttaatt aacagcaaag ggcagaatgt ttctgccatc tatggatttt tcaggatgct
      721 gcttcgtttg ttgcaaacgc agaaacctga ttacacaatc attgcgtttg acccgccaaa
      781 aaaatcattc cgattttcta tgtatcctga gtataaagca cagcgtgcaa aaatgcctga
      841 tgatttgcgc agtcagattg aagaaatcaa agctatattg caggagatgg gatttaccgt
      901 tgtcataatg gatgattatg aagctgatga tgtgcttggt tctattgccc gtcagtattc
      961 ggatagctac aaagtggtac ttgttaccgg tgataaggac gcttaccagc ttttgagcga
     1021 taagaacgtc tcaatctatg ccggcaaaaa aggtatcact gaatttgaaa gcatagatgc
     1081 tgatgctgtt gttgaaaaat taggtgttag accagaacaa atcattgact atatgtcact
     1141 caccggagat gctgtagata atatccctgg tgttaaaggc attggcgaaa aaactgcatc
     1201 aaaacttttg cagcagtatg gaacgttaga taatatttat aagcatttgc atgaacttac
     1261 cggaaaaatt aaggaacaga ttgaatcaca gaaagactca gcgtatcttt cacgtcaact
     1321 tgtgacgata aaaactgata taccgttatc actttccatt gaaaaggctt tgacccctga
     1381 tttacacaat gaaaaagttc ggcagatttt caaaaattta gagatggatt caattgcccg
     1441 tgaattgggc gatacagaaa aatcaatttc aattaaggaa atagcaggat attccacagt
     1501 aacagatatt gatacactga acaatgtcat tgcagcaatc aaaaacgcag gacttgtgtc
     1561 atttgatacc gaaactacat catcacgacc aatggaagca gagcttgtgg gagtatcgtt
     1621 ttccattgca ccgcaatcag ggtggtttat tccagtgggc gatagttccc tgtggggtta
     1681 taatgcagta ccaaaagatc agctgcttca aaaaattaaa actatccttg aagatgatac
     1741 aataaagaaa gttggtcaaa atataaagta tgacatgctg attatgctta attgtggtat
     1801 tacgcttaca ggcatctatt ttgacaccat ggttgcatca tatttactta atcccggatt
     1861 acggcggcat aatctggatg atttagcagc agaatatctc aattatacaa agataacgta
     1921 tgatgaactt gtgaaagatg gcaaaaaggt gcagcatatt acgcaggtgc cattgcagaa
     1981 actgtcccac tacgctattg aagatgctga tattgctttg cgcttatacc atatactcta
     2041 taaaaagtta gaacaggaaa acctgcttga attgtttttc aacgttgaaa tgccacttgt
     2101 tactgtactt gcgtacatgg aatattacgg agtaaagatt gaccttgaac actttgaaac
     2161 attatctaag gaactatcgc acaaaataaa acaaaccgaa gaaaaaattt actcactggc
     2221 aggaattcca tttaacatca actcaacgcg ggaactatcg cacatattgt ttgaaaagat
     2281 tgggcttaaa ccggtaaaga aaacaaaaac tggtttttcc accgatattc aggtgctgga
     2341 agctttgagg ggacagca
//
LOCUS       DAOSZP010000367         2356 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_34735_length_2356_cov_2.522816, whole genome shotgun sequence.
ACCESSION   DAOSZP010000367 DAOSZP010000000
VERSION     DAOSZP010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34735_length_2356_cov_2.522816"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="PK348_08085"
     CDS             complement(<1..201)
                     /locus_tag="PK348_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HXK66210.1"
                     /translation="MFFLKKLITSAMLPPGIFIIMFIIIGVWVKRKSITILSLLCALL
                     LYALSIEPVKDILFYHLEKQAYT"
     gene            603..1658
                     /locus_tag="PK348_08090"
     CDS             603..1658
                     /locus_tag="PK348_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015589647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase family protein"
                     /protein_id="HXK66211.1"
                     /translation="MCGIVGIINTDRTKIDGSYIREAIRIQNERGNGLGGGFAAYGIY
                     PEHKDNYAVHIMYQGGRHSDIIKKIENFLEQKTKVHHSEEIPVYPTSIIPSGPYFKRY
                     FVNPLKEQIPVGKNEDDAMVDIVMTINRMGDAYVISSGKDMGVFKGVGFPEDIADFFR
                     IQDYKGYMWIAHNRFPTNTPGWWGGAHPFTILDKAVVHNGEITSYGTNKRWLEMFGYF
                     CMLQTDTEVISYIFDKLTRKDGLSVEDACFVMAPSLWEDIEHENAHKKMLLRQLYGQA
                     IVNGPFGIVLTHSGGAIVLNDRNKLRPVVCARVGSTYYASSEECSIRLLSPKVDELWS
                     PKGGEAVIVNLAKEEAC"
     gene            1658..>2356
                     /locus_tag="PK348_08095"
     CDS             1658..>2356
                     /locus_tag="PK348_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HXK66212.1"
                     /translation="MASMKNNWKTIPPFHPEIDYSRCDYKNGCNICAHECSWGEIALK
                     PEKLDNGTVVYKPVANLTSCGSCQRCVKMCPYNAIHIVNSPMYTSHGYWTPDYVGNIW
                     KQASSEGGVLLVGNGATGPQRRYFDHLMFDACQVTNPSIDPLREPMEIRTYLGRRPEK
                     WGQDTYPLLKLEVPIMFGAMSFGALNLRVHEAEARATKEIGTYWNTGEGGFHRSLREK
                     YGSNTIVQVASGRFG"
BASE COUNT          713 a          405 c          530 g          708 t
ORIGIN      
        1 cgtgtatgcc tgtttttcaa gatggtagaa tagaatatct ttcacaggtt caattgatag
       61 tgcatagagt agcagcgcac atagtagcga cagtatggta atgcttttgc gttttaccca
      121 aaccccgatg ataataaaca taataataaa aatgcctggt ggcaacattg cactggtgat
      181 gagcttcttt aaaaaaaaca tggtgattct cccggttact atcgcccaaa attattacac
      241 attcttttga tgtaaagcac tatactgcaa aatattttgt gattttataa aaattataat
      301 gatgatacct atttataatc ttgattagtt tgttttttat tattattttg tgataacatc
      361 ccatgatatt tttttaaagg caaaatagct ttggagatta tgtagtatgt ttttttaaaa
      421 gggaattttg tataggtaat actttacttt actaaacata actatataat gaataaaaaa
      481 ttttatccga atatgctcgt aaaatttaac attgctgtaa catagctgca accttttgta
      541 ttgaattacg ggatgcgtgc gctacatatg gttcttacca ctaaacaagt aaagagggtg
      601 ttatgtgtgg aatagtagga ataataaaca ctgacagaac aaagattgat ggcagttata
      661 tacgtgaagc aatccgtata cagaatgaac gaggaaacgg ccttggtgga ggttttgccg
      721 cctatggcat ttatccagaa cataaagata attatgctgt tcatatcatg tatcagggag
      781 gaaggcacag cgacattata aaaaaaattg aaaacttttt ggaacaaaaa accaaggttc
      841 atcactctga agaaattcca gtatacccaa caagcataat tccttcaggg ccatacttta
      901 aacgctactt tgtcaatccg ttgaaggaac agatccctgt gggtaagaat gaagatgatg
      961 caatggttga tattgtaatg accattaaca ggatgggtga tgcctatgtc atatccagtg
     1021 ggaaggatat gggtgtattt aaaggtgtgg gcttccctga agatattgct gatttttttc
     1081 gtattcagga ttacaagggg tatatgtgga ttgcccataa ccggtttcct accaatactc
     1141 ccggatggtg gggtggtgct cacccgttta ccattcttga caaagctgtt gttcacaatg
     1201 gcgaaataac cagttatggt accaataaac gctggttaga aatgtttggt tatttctgca
     1261 tgctccagac agataccgaa gtaataagct acatctttga caagttaaca cgcaaagatg
     1321 gccttagtgt tgaggatgcc tgttttgtga tggctccttc attgtgggaa gacattgaac
     1381 atgaaaatgc acataaaaaa atgcttctta ggcaattata cggtcaggcc attgttaatg
     1441 ggccttttgg aattgtatta acacattctg gtggggcgat agttcttaat gatcgaaaca
     1501 aactgcggcc agtggtatgt gctcgtgttg gttcaaccta ttatgcgtca tcagaggaat
     1561 gcagtatacg gttactatct cccaaagttg atgaactgtg gtcgcccaaa ggtggtgaag
     1621 ctgttattgt taatttagca aaagaggagg cttgttaatg gcatcgatga aaaataactg
     1681 gaaaaccata cctcctttcc accctgagat tgattattca cggtgtgact ataaaaatgg
     1741 atgtaatata tgtgcccatg aatgctcatg gggcgagatt gcattaaaac cggaaaagtt
     1801 agataatggt acggtggtgt acaagcctgt agcaaacctt acatcgtgtg gttcttgcca
     1861 gagatgtgtt aagatgtgtc cgtataatgc aattcatatt gtcaacagtc ccatgtacac
     1921 cagccatggt tactggactc ccgattatgt gggcaatatt tggaaacagg cttcatccga
     1981 aggtggtgtg ctgttagtgg gcaatggtgc aacaggtccc cagcggcgct attttgacca
     2041 cctgatgttt gatgcctgcc aggttaccaa tccttccatt gacccattgc gtgaaccaat
     2101 ggaaatccgc acgtatttag ggagacgccc tgaaaagtgg gggcaggata catacccgct
     2161 tcttaaatta gaagttccca taatgtttgg tgcaatgagt tttggtgcat tgaatctgcg
     2221 ggtacacgag gctgaagcaa gagcaacaaa agagattggt acatactgga ataccggtga
     2281 gggtggattc cacaggtcac ttcgggaaaa gtatggttcc aatacaatag tacaggttgc
     2341 atctggccgt tttggt
//
LOCUS       DAOSZP010000368         2353 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_34783_length_2353_cov_3.067015, whole genome shotgun sequence.
ACCESSION   DAOSZP010000368 DAOSZP010000000
VERSION     DAOSZP010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2353
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34783_length_2353_cov_3.067015"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1838
                     /locus_tag="PK348_08100"
     CDS             <1..1838
                     /locus_tag="PK348_08100"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HXK66213.1"
                     /translation="VVAVFPLFNVFSNNNEQLLEDILGIKHKDLYTLDTVANKAKWID
                     GQVIVKYKDTIATSKALQKLAKKAKHVSALHRKPLFNVILKDMRVEDAIRELSKDPDV
                     EYVQPNYIYHMLAVPNDPAFYMQWALKNTGQKVEGGYTINNPGTLGCDANVEKVWDMH
                     SDCSNIIVAVLDSGVNYIHQDLKDNMWDGSPYGYPYHGYNFVYDDNDPMDYHGHGTHV
                     AGIIGAKGNNNVGISGVCWKAKIMAVKVLTDEGYGSTSTIARGIYFAVDNGAKIINMS
                     LGGSVYDTAFHFAINYAASKGVLVVAAAGNNGMGLYESMLNPIYPCMFKSDNILCVGA
                     LDQNDIKATFSNYSYKDTVVHIYAPGVNIVSSFTSTVAEQITGNMINQSGWQMNDSNW
                     AIHNTLGYTVASNPANFDFTVTYKPNQNSYLYRVVDCTGYKKVSMIYEMYYDVENYYD
                     AFRVYYAKGQQNPLYMNTSIFTLLATIAGKSDGFKTYMHNLPNCRNALCTVVYNLSSD
                     SVVNYKGVAIKSAGLYNLKPVDDRYAVLMGTSMATPMVSGAAALVWAMFPDSNYKAIK
                     ERLINGAQKLLLYSGSFSYTINKLNVWGAVTYIKPPANVQYKKIN"
     gene            1851..2261
                     /locus_tag="PK348_08105"
     CDS             1851..2261
                     /locus_tag="PK348_08105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66214.1"
                     /translation="MKKIIIPLFVIMFFIACSEYEEESANKNDDYLTYQYEISWDAND
                     EDGVNSFGGGYCVYYAEGYAVDINKSNYVDVPYQGGDRAPTQATIMIKLKKDENSNYK
                     RQVTYSVVVVAYATINGTKVYSRPSKQIQITLTI"
BASE COUNT          802 a          329 c          499 g          723 t
ORIGIN      
        1 tagttgttgc tgtgtttcct ttgtttaatg tattcagcaa taataatgaa cagttgcttg
       61 aagatatttt aggtatcaaa cataaagatt tatatacact ggatactgtt gcaaataaag
      121 ctaaatggat tgatggtcag gttatagtta aatataaaga tactatcgcc acttcaaaag
      181 ctttacaaaa acttgcaaaa aaagcaaagc acgtatctgc attacaccgc aagccattat
      241 ttaatgtaat tctgaaagac atgagagttg aagatgccat tcgtgaatta agtaaagatc
      301 ctgatgttga atatgtacaa cccaattata tatatcacat gcttgctgtt cccaatgatc
      361 ccgcatttta tatgcagtgg gcattaaaaa atacaggcca gaaagtagaa ggaggttaca
      421 caataaataa tccaggaaca ttgggatgtg atgcaaatgt tgaaaaagtg tgggatatgc
      481 attctgactg cagcaatatt atagtggctg tgttagatag tggtgttaat tacatacatc
      541 aggatttaaa agacaatatg tgggacggtt caccatacgg ttatccatat catggatata
      601 attttgttta tgatgacaat gatccaatgg attatcatgg tcatggtaca catgttgcag
      661 gaataatagg ggcaaaaggg aataataatg taggtataag tggtgtgtgc tggaaggcaa
      721 aaatcatggc agtgaaagtg ctcactgatg aagggtatgg ttccacttca actattgcac
      781 ggggaatata ttttgcagtg gataacggtg caaagattat caatatgagt ttgggtggaa
      841 gtgtgtatga tactgcattt cattttgcca taaactatgc tgccagtaaa ggtgtgctgg
      901 ttgttgctgc agcgggtaat aatgggatgg gattgtatga aagtatgtta aaccccatat
      961 atccctgtat gtttaaatca gacaatattc tgtgtgttgg tgcactggat cagaatgata
     1021 taaaggcaac attttcaaat tattcataca aagatactgt tgttcatata tatgcacctg
     1081 gtgttaatat tgtaagttca tttacatcca cagtggctga acaaataaca ggtaatatga
     1141 ttaatcaaag cggctggcaa atgaacgata gtaactgggc aatacataat accctggggt
     1201 ataccgttgc aagcaatcca gcaaattttg attttactgt tacctataag ccaaatcaaa
     1261 acagctatct ttatagagta gtggattgta ccgggtacaa gaaagtttca atgatatacg
     1321 aaatgtacta tgatgttgaa aattattatg atgcatttag agtgtattat gcaaaaggac
     1381 agcaaaaccc attatatatg aataccagta tttttacttt gctggcaacc attgctggta
     1441 aaagtgatgg atttaaaaca tatatgcata atcttcccaa ttgccgaaat gcattatgca
     1501 cagttgtcta taacctaagt agcgatagtg tggttaatta caaaggtgtg gcaattaaaa
     1561 gtgcaggttt gtataatctt aagccagtgg atgacaggta tgcagtgtta atgggcactt
     1621 caatggcaac accaatggta agtggagcag cggcactggt atgggcaatg ttccctgaca
     1681 gcaattataa agccataaag gaaaggttaa taaatggtgc tcaaaagtta ttattgtatt
     1741 ctggtagttt cagttatacc ataaataaac ttaatgtatg gggtgcagtg acgtatatta
     1801 agccgcctgc aaatgtacaa tataaaaaaa taaattaacg aggtggtttc atgaaaaaaa
     1861 ttataatacc attatttgtc attatgtttt ttatagcctg tagtgaatac gaagaagaaa
     1921 gcgctaataa aaacgatgat tatttgacat accagtatga aatatcctgg gatgctaatg
     1981 atgaggatgg tgttaatagt tttggtggtg gttattgtgt gtattatgcg gaagggtatg
     2041 cagtagatat taataaaagc aattacgtag atgttcctta tcaggggggc gatagggctc
     2101 ccacacaggc aactattatg attaaactaa aaaaagatga aaatagcaat tacaaaaggc
     2161 aggttactta cagcgtagtg gtggtggcat acgcaacaat aaatggcact aaagtgtata
     2221 gcagaccttc taaacagatt caaattactt tgactatata ataattattt acctggaggt
     2281 tttaattatg tcaaaccatt gttttaatct tgaaaacaga gatttgatgt ttgttcgttc
     2341 tacacgttca ttt
//
LOCUS       DAOSZP010000369         2340 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_35000_length_2340_cov_2.956236, whole genome shotgun sequence.
ACCESSION   DAOSZP010000369 DAOSZP010000000
VERSION     DAOSZP010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35000_length_2340_cov_2.956236"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /locus_tag="PK348_08110"
     CDS             complement(<1..267)
                     /locus_tag="PK348_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020886322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXK66215.1"
                     /translation="MSESTVKVKAQNLSFFYGDNQALFDINLSVQANTVMALIGPSGC
                     GKSTFLRCINRMNDIIPYTRIEGELTIDGESIYHTKYDVTQLRRR"
     gene            579..1406
                     /locus_tag="PK348_08115"
     CDS             579..1406
                     /locus_tag="PK348_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013257882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HXK66216.1"
                     /translation="MKVKSFLIFMIVAIVSSLSIGFSQTKGSKVVIKGSTTVLPIAQK
                     ALEAFYEKTNVVISLSGTGSGDGIKSLIDGSCDIANSSREMKKEEWDMAKSKGEKIKE
                     VAIAYDMIVPIVHPSNPVKNLSMAQLKAIYEGAITNWSQVGGNNERIVVISRDSSSGT
                     FEVWESKVMKKSEVRKDALLQASNGAIVSVVSKNKRSIGYVGYGYLDDRVKALTVNGV
                     EPTIPNGKSGAYPIARKLYMYVNEVKIKPEAQAFIDFILSKEGQQIVKAAGFIPLTD"
     gene            1529..>2340
                     /locus_tag="PK348_08120"
     CDS             1529..>2340
                     /locus_tag="PK348_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010939747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HXK66217.1"
                     /translation="MKIKRYTVLFILFIGIAALIVSCSKEAREKVVVKGSTTVLPITQ
                     KAAEAFYEKNKIVISLSGTGSGDGIKSLIDGSCDIANSSREMHTEELALAKRKGEIIK
                     EVAIAFDMIVPIVHPSNPVSNLTLHQLKAIYEGSVKNWKDVGGKDETIVVISRDSSSG
                     TFEVWESKVMKHVEVRKDALLQASNGAILQTVSQNPKAIGYVGYGYLNDRVKPLYVNN
                     VEPIIENGKSGKYPIARKLYMYVNEVKIKPAAQAFIDFMLSDEGQSIVKEAG"
BASE COUNT          794 a          359 c          497 g          690 t
ORIGIN      
        1 gcggcgacgc agttgtgtga catcgtattt tgtatggtat atagattctc catctatggt
       61 tagttcgcct tcaattcttg tatagggtat gatatcgttc atcctgttga tgcagcgtag
      121 aaatgttgat ttaccacatc cagaaggacc aatcagtgcc ataactgtgt ttgcctgtac
      181 tgatagatta atatcaaaca atgcctgatt atccccgtaa aaaaaagaaa gattctgggc
      241 tttaactttt actgtgcttt cggacatata tcaccgttat ttctttaact agtacgttgt
      301 atgtatcatt acttttaatt atcaaaagta tcaaaccact ggaatgttaa ggatttataa
      361 taataatgta aagaaaatgt aaagttttag tactatagca gtgctttttg ccgcctggac
      421 ttattgttgt atatgatgaa aagatcaatt caggtcaaat caaatagaaa attaatctaa
      481 tctttataaa aatttcataa taccagtgta cttagaatag tgtgaaattg cataattgcg
      541 ttttacatta ccttactaaa atataacagg aggacataat gaaggtcaag agttttttaa
      601 ttttcatgat agtagcgata gtaagctcat tatcaattgg tttttcacaa acaaaaggat
      661 caaaggtagt catcaaagga tcaactaccg ttttgcccat tgctcagaag gcactggaag
      721 ctttttatga aaaaacaaat gttgtaataa gtctttcagg tacaggttct ggtgatggta
      781 taaagtcgct tatagatggt agctgcgata ttgcaaattc ttcacgtgaa atgaagaagg
      841 aagagtggga tatggcaaaa tcaaaaggtg aaaagattaa agaagttgct atagcatacg
      901 atatgatagt tccaatcgtg catccttcaa accctgtaaa aaatttgtca atggctcagc
      961 ttaaagccat ctatgagggt gctataacca actggagtca agtgggtggc aacaatgaaa
     1021 ggatagttgt aatatcccgt gattcaagtt caggaacgtt tgaagtgtgg gaatcaaagg
     1081 ttatgaagaa atctgaagtg cgcaaggatg cgttattgca ggcttccaat ggagcaattg
     1141 tatccgtagt atcaaagaat aagcgttcaa ttggatatgt tggatacgga tacttagatg
     1201 acagagtaaa agcacttact gtaaatggag ttgagccaac tatccctaac ggaaaatcgg
     1261 gtgcgtaccc aatagcacgt aagttgtaca tgtatgtcaa tgaagtcaaa ataaaacctg
     1321 aagcgcaagc attcatagat tttatccttt caaaagaagg acaacagatt gtaaaagcag
     1381 caggttttat ccccttaact gattgatgtg aacattaaac acttattaaa aaacgctaca
     1441 gcgatgtggc gtttttttgt aaaaaagtgt tgaatatatt gggaatgatg aggtaacatt
     1501 aaattatatt tttcatggag gtttgaagat gaaaattaaa cgctacacag tactatttat
     1561 actatttatt ggcatagcag cacttattgt atcgtgttca aaagaagcca gggaaaaagt
     1621 agtggttaaa ggttcaacga cggtgttacc cattactcag aaggctgctg aagcattcta
     1681 tgaaaaaaat aaaattgtca taagcctttc aggtactgga tccggtgatg gtataaagtc
     1741 gctgattgat ggaagctgcg atattgcaaa ttcttcgcgt gaaatgcata cagaagagct
     1801 ggcgcttgca aaacgcaaag gtgaaatcat caaagaagtt gccatagcat ttgatatgat
     1861 tgtgcctatt gttcatcctt caaatcctgt ttcaaacctt accttacatc agcttaaggc
     1921 aatttacgaa ggttcggtga aaaactggaa agatgttggt ggtaaagatg aaaccattgt
     1981 ggttatatca cgcgattcaa gctcaggaac atttgaggtg tgggaatcca aagtaatgaa
     2041 acatgttgag gtgcgcaaag atgcactgct gcaggcttca aatggggcaa ttttacagac
     2101 agtatcgcaa aatccaaaag ccattggata tgtggggtat ggatatctta atgacagggt
     2161 taagccgctg tatgtgaaca atgtggaacc aataatagaa aatggtaaaa gtggcaaata
     2221 tcccattgca cgtaaactct atatgtatgt caatgaagta aagataaaac ctgctgctca
     2281 agcatttata gactttatgc tgtcagatga aggacaatca atagtgaaag aagcagggtt
//
LOCUS       DAOSZP010000370         2334 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_35125_length_2334_cov_3.032470, whole genome shotgun sequence.
ACCESSION   DAOSZP010000370 DAOSZP010000000
VERSION     DAOSZP010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2334
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35125_length_2334_cov_3.032470"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            351..>784
                     /locus_tag="PK348_08125"
     CDS             351..>784
                     /locus_tag="PK348_08125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 43"
                     /protein_id="HXK66218.1"
                     /translation="MKIKDFLHLTFKEYEGNPIIAPPFPSPIIADPTFVPPNESPDGK
                     WHLFAHSLMGIHHFVSDDGINYTRMSKHTIGNAMRAFLYYEDNMYYLYYEKIKPYILW
                     YSWLPHTWYSYIAMRSSHDLYSWSDEQIILKPSLPWHSNTFGA"
     assembly_gap    785..794
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <795..1273
                     /locus_tag="PK348_08130"
     CDS             <795..1273
                     /locus_tag="PK348_08130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl hydrolase family 43"
                     /protein_id="HXK66219.1"
                     /translation="SAALTHIQDCGFNEPLYIGLAQSSNPAGPFQSLPHPIMEPSHYN
                     RWCNIGCGSFKALKMDDGFIGLQNGIYWDYTINQSGSAILVYSSKDGIHYTSLMKNPL
                     LKPDIKEGSYKRSHVYAFDLKQVDKNHWYLYFNARNNWHWSKGKEHIALLIGSKTS"
     gene            complement(1338..2279)
                     /locus_tag="PK348_08135"
     CDS             complement(1338..2279)
                     /locus_tag="PK348_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009108066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HXK66220.1"
                     /translation="MFKDISENGDYQKHLEHVVKTLCKPESYTKVYHRTNHDVPMPSI
                     PQLAEIVEECKLVLFPGYFGNSEITPQNMQYYIGSALDSIARKLTEQIKRGYCFSCAE
                     GETMDCIECEHRSRKIMFQFLDMLPEIRRMLALDVQAAYEGDPAAKSHGETIFCYPSI
                     MAMINYRIAHALYILDVPLIPRIITEMAHSQTGIDIHPGATIGEKFFIDHGTGVVIGE
                     TTIIGNNVRIYQGVTLGAKSFPLDENGNPIKGIPRHPIVEDDVIIYSGATILGRVTIG
                     KGSVIGGNVWLTESVPPGTHVLQQKPMEMLFENGGGI"
BASE COUNT          710 a          483 c          432 g          699 t
ORIGIN      
        1 atcgatagat actgtactac agtactcatc tttgttggta gttgtcaact gccaggtgcc
       61 ctcaagtgat tgtacaatct ccactgaagg agtacaggca gttaaagcta ttgcagcata
      121 tagcagaatt attatcgtaa ctatcggttt catacagcac actattgtaa tgaacataaa
      181 acatataaac aacaaaaagt caaaaacttt attttcaaaa tgtcaacaat aaggcttgac
      241 gtagtatatg tatagtatgt acaattagca ggtagtgtta aaggcagtaa attattttgt
      301 ggcgatagct acttataatg aactatcgcc cacataaaaa agagcatgct atgaaaataa
      361 aagatttttt gcaccttacg tttaaagaat acgaaggcaa tcccattatt gccccaccct
      421 tcccatcgcc gataattgcc gatccaacgt ttgtaccacc taacgagtca cctgatggca
      481 aatggcattt atttgcacat tccctgatgg ggatacacca ttttgtttca gatgacggca
      541 tcaattacac caggatgtca aagcacacca ttggcaatgc tatgcgtgct tttctttatt
      601 atgaagataa catgtactat ttatattacg aaaagattaa accgtacatc ttatggtatt
      661 catggcttcc acatacatgg tattcatata ttgcaatgcg ttcttcacat gatttatatt
      721 catggagtga tgaacaaata attttaaaac catcacttcc ctggcatagt aatacatttg
      781 gggcnnnnnn nnnnacagtg ctgcattaac ccacatccag gattgtggat ttaatgaacc
      841 actgtatatt ggactggcac aatcttcaaa tcctgcagga ccttttcaaa gccttcccca
      901 ccccatcatg gagccatcac actataaccg atggtgcaat attgggtgcg gttcatttaa
      961 agccctgaaa atggatgatg gtttcattgg gttgcagaac gggatttact gggattatac
     1021 tataaatcag tcgggttcag ccattttagt atactcatca aaagatggta tacactacac
     1081 ctcgttaatg aaaaacccgt tgttgaaacc tgatatcaaa gaaggttcat ataaacgaag
     1141 ccatgtctat gcatttgatt taaaacaggt tgataaaaac cattggtatt tgtatttcaa
     1201 tgctcgcaat aactggcact ggtcaaaggg gaaagaacac atagcattat tgataggcag
     1261 caaaacatca taaacggcac tattacaaaa gcatctccca ttttgtaact aagattactg
     1321 ttttagaaaa tgtattgtta tataccacca ccattttcaa aaagcatctc cattggcttt
     1381 tgctgcagca catgagtccc cggtggaacg ctttcagtta accatacatt tccacctata
     1441 actgacccct tgccaatggt tactctgcct aatatggttg cacctgaata tatgataaca
     1501 tcgtcttcaa caattgggtg gcgcggtatt cccttaatag ggttgccgtt ttcatcaagc
     1561 gggaagcttt ttgcacccaa tgttacaccc tgatagatgc ggacattgtt gccaataatg
     1621 gtggtttcac ctatgacaac acctgttcca tggtctatga aaaatttttc accaatggtg
     1681 gctccagggt gtatatcaat ccccgtctgt gaatgtgcca tttcggtgat gatacgcgga
     1741 atgagtggca catccagaat atacagagca tgggcaatcc tgtaattaat cattgccatt
     1801 atgcttggat agcagaatat ggtttctcca tggcttttgg cagcagggtc accttcatag
     1861 gctgcctgta catccagggc aagcatccga cgaatctctg gtagcatatc taaaaactga
     1921 aacatgattt tccgtgaacg atgctcacac tcaatgcaat ccatcgtttc accttctgca
     1981 catgagaaac agtacccgcg ttttatctgc tctgttagtt tgcgtgcaat actatctaaa
     2041 gctgaaccta tataatattg catgttttgg ggggttatct cagaattgcc aaaataacca
     2101 ggaaacagta ccagcttaca ttcttcaaca atttccgcca gttgtggtat ggaaggcatg
     2161 ggaacatcat gattggttcg gtggtatact tttgtatacg attcaggctt gcataatgtc
     2221 tttacaacat gctccaggtg cttttgataa tcgccgtttt cagatatatc tttaaacata
     2281 ctgtaaacca gaaaattaat aataccttgc aggtagcaat tatatcacat atac
//
LOCUS       DAOSZP010000371         2320 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_35362_length_2320_cov_3.492274, whole genome shotgun sequence.
ACCESSION   DAOSZP010000371 DAOSZP010000000
VERSION     DAOSZP010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2320
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35362_length_2320_cov_3.492274"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /gene="rnpA"
                     /locus_tag="PK348_08140"
     CDS             <1..255
                     /gene="rnpA"
                     /locus_tag="PK348_08140"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HXK66221.1"
                     /translation="QAEGNMNNILQSRFAIVVTKTYGNAVERNRIKRQIRSILVTLMP
                     QMRQGFAVIIFPDTTCKAMQYNHLVVSVKQVLLKSGVLKQ"
     gene            264..473
                     /gene="yidD"
                     /locus_tag="PK348_08145"
     CDS             264..473
                     /gene="yidD"
                     /locus_tag="PK348_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HXK66222.1"
                     /translation="MVTIPVVLIKLYKLVISPVLPNACRFYPTCSQYAIEALTRYGLF
                     KGGFLSIKRILRCHPFCDGGYDPVP"
     gene            589..2211
                     /gene="yidC"
                     /locus_tag="PK348_08150"
     CDS             589..2211
                     /gene="yidC"
                     /locus_tag="PK348_08150"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HXK66223.1"
                     /translation="MEKRALIAVLLSLGVWILWFYLFPPQEKTPPVPEKSAETATVQT
                     EEIQKPEISVVSKFQQTFTKKEEVTIDAKYYTAVLSNEDGQCTSFIDKVRNIELIVTK
                     SKFDAKGFFDFPVHFSTGEFLHAKDIPPALWKMQKDASAVRFSTVAIVQKNPLEISKK
                     FTYNETGQYFVVSYAITNKGQSEFTFPEGGVIFSPSDFLGPSMDFDNSYNELHGIYYI
                     NDSFEKARKGGGFFSAPEDLKRENGVVQWAGIMSRYYLLIMIPQGFAGSGVVYDNREH
                     TGFRTGIMVPVKPLKPGETITHEFKVYVGPKNKDKLLAVDKHIGDAADVSKWIEPIRD
                     FMMWCLLEINRLIGNLGWSLVIFSVITKIMFLPLTQRSTESMKKMQELNPVIQELREK
                     YKDKPDVLNKKIMEVYKKNKVNPMGGCLPLLLQMPFFFALYSALINSVDLWQAPFILW
                     IKDLSLPDTIYRIAGFNINILPLVMTGTTYLQQKMSSSEVTQQQKMLMLMPLIFIVIF
                     WNMPSGLVLYWIMQNVLQIGHQMLINKIGKKK"
BASE COUNT          744 a          360 c          480 g          736 t
ORIGIN      
        1 caggcagaag ggaacatgaa caatatatta caatctcgtt ttgccatagt ggttactaaa
       61 acctatggaa atgcagttga acggaataga ataaaaaggc aaattcgttc cattttggtt
      121 actctgatgc cccagatgcg acagggtttt gctgttatta tatttccgga caccacctgt
      181 aaggctatgc aatataatca tcttgttgta tcagttaaac aagtattgct taaatcagga
      241 gtactaaaac agtgaagata aatatggtta ccataccggt agtgttaata aaattatata
      301 aacttgtgat ttcaccggta ttacccaatg cttgtagatt ttatccaacg tgttctcaat
      361 atgcaattga ggcattgacc aggtatgggc ttttcaaggg tggtttttta tcaattaaaa
      421 ggatacttcg ctgtcatcca ttttgtgatg gtgggtatga ccctgtcccc tgattatcat
      481 ttttaagtac ggaaaaatat tcccttcctg ttatctcctt tccacggtat gctagtatac
      541 atatgtggta ttgttgtacc tgagtaaaaa atttatcaag aggttgtcat ggaaaaaagg
      601 gctttaattg ctgtgttatt atctttaggt gtatggatac tgtggtttta tttatttccg
      661 ccacaggaaa aaacacctcc ggtcccggaa aaaagtgctg aaactgcaac agtgcaaact
      721 gaagaaatac aaaaacctga aatttctgtg gtttcaaaat ttcagcaaac ttttacaaaa
      781 aaggaagaag ttaccattga tgcaaaatac tatacagcag tactttccaa tgaggatgga
      841 caatgtacat cgtttattga taaagtaaga aatatagaat taattgtaac taaatcaaaa
      901 tttgatgcaa aaggtttttt tgatttccct gttcattttt caacaggtga gtttttgcat
      961 gcaaaggata tacctcctgc attatggaaa atgcaaaaag atgcatcagc agtgcgcttt
     1021 tccacagttg ctatcgtaca aaaaaatcca ctggaaattt caaagaaatt tacgtataac
     1081 gaaacaggac aatattttgt tgtatcgtat gctatcacta ataaaggaca gagtgaattt
     1141 acatttcctg aagggggtgt aatattttcc ccatctgatt ttttagggcc atcaatggat
     1201 tttgataatt catataatga gttacatggt atctattata ttaatgatag ttttgaaaaa
     1261 gcccgtaagg gtggcggatt tttttcagca ccagaagacc tcaagaggga aaacggtgtg
     1321 gtacaatggg ctgggataat gagcaggtat tatctgttaa ttatgatacc acagggtttt
     1381 gctggttcag gtgtggtata tgataacagg gagcatacgg gtttcaggac gggtattatg
     1441 gtaccggtca aaccactgaa acctggtgaa acaataacac atgaatttaa agtatatgtt
     1501 ggtccaaaaa ataaagataa gcttcttgct gttgacaagc acattggtga tgctgcggat
     1561 gtcagcaagt ggattgaacc tatcagggac tttatgatgt ggtgcctgct tgaaataaac
     1621 aggctgatag gaaatttagg gtggtcactg gtaatatttt ctgttattac aaaaataatg
     1681 tttttaccat tgactcaacg ctccacggaa tccatgaaga agatgcagga attgaatcct
     1741 gtgattcagg aattgcgtga aaaatataag gataagcctg atgttttgaa taaaaagata
     1801 atggaagttt acaaaaagaa taaagtgaat ccaatgggtg gttgtttgcc gctgcttttg
     1861 cagatgcctt tcttttttgc actgtacagc gctctgataa attcagtaga tttatggcag
     1921 gcgcccttta ttttgtggat aaaggatttg tcattgcctg atacaattta caggatagct
     1981 ggatttaata ttaatatttt gcccctggtt atgacaggaa caacatatct gcagcaaaag
     2041 atgtcttcat ccgaagtcac tcagcaacag aagatgttaa tgctcatgcc gttaatattt
     2101 attgttatct tctggaatat gccctcgggg ctggtattgt actggattat gcaaaatgta
     2161 ttgcagatag gccatcagat gcttattaat aaaattggca aaaaaaagta atacaaattt
     2221 ttgtgtgaaa tgtgaggatt ttataagtac attatcttca ggaaagttac ccatagtcgt
     2281 tacaaccacc atgcgaagtg gttaatatat taatgaggtt
//
LOCUS       DAOSZP010000372         2320 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_35370_length_2320_cov_2.669757, whole genome shotgun sequence.
ACCESSION   DAOSZP010000372 DAOSZP010000000
VERSION     DAOSZP010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2320
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35370_length_2320_cov_2.669757"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..919
                     /locus_tag="PK348_08155"
     CDS             <1..919
                     /locus_tag="PK348_08155"
                     /inference="COORDINATES: protein motif:HMM:NF025112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HXK66224.1"
                     /translation="FRACRLAAKLHFSIHKKTLEAIPQVLEIAKKISVERVRDEIKKM
                     LEADKPSEGFEYMRVTGLLDLYMPELSQGFGMEQNKYHMYDIYYHSIYSCDAAPKEKP
                     LVRLAALLHDIGKIPTRREGSDGDYTFYNHEVISSKIARKIMRRLKFSNDEIEYVTNL
                     IANHMFHYTNDWTDGAVRRFIRKVGLDNLDDLFLLRLADRCGNGMRDGLPEPIKKLKR
                     HIQRVIEKDNAFTVKDLDIDGYILMEHLKLKPGPIIGKILNELLEQVLDNPELNNQET
                     LLQMAHELYAAMANDTTATCHDSTQHHIV"
     gene            complement(916..>1073)
                     /locus_tag="PK348_08160"
     CDS             complement(916..>1073)
                     /locus_tag="PK348_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004492547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HXK66225.1"
                     /translation="KALIKELIPLLTKERDCGCKEAIKYAIITDPKKQSSKQKKKLKA
                     ILPNYFS"
     assembly_gap    1074..1083
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1084..1828)
                     /gene="mtnP"
                     /locus_tag="PK348_08165"
     CDS             complement(<1084..1828)
                     /gene="mtnP"
                     /locus_tag="PK348_08165"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016549674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HXK66226.1"
                     /translation="MKPKIDVGIIGGSGLYQIEGVTILEEVKVRTPWGMPSDAITIAE
                     VGGVKAGFLPRHGRGHFILPHEINYRANIASLKMLGAGQIVAFSAVGSLKERIKPLDF
                     VIPNQIIDRTKSRISTFFGDGIAAHIAFADPFCTRLHELILVAAQKLNIPMHTNETLV
                     CMEGPAFSTRAESHLYRSWQAGVINMSTLPEAKLAREAEMCYAVICMSTDYDCWKEDE
                     EHVTVDMVVNNLNKNASNARGSTDNSFDFI"
     gene            complement(1880..>2320)
                     /locus_tag="PK348_08170"
     CDS             complement(1880..>2320)
                     /locus_tag="PK348_08170"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXK66227.1"
                     /translation="ELPIYKTAYLPKELEASSQPKDLSNLTVLIVDDDDAIRDLFKTL
                     LQSLGISAITFATNGYEGYSLCKENQYDIVFMDVSMPVLSGIDAYKMIKDENPSQKVV
                     FITGIFYEDQIKELVDKEQAFGYIKKPFDIKEIKSLLFTIASHQ"
BASE COUNT          703 a          438 c          457 g          712 t
ORIGIN      
        1 attcagagca tgccggttag ctgcaaaact acatttcagc attcataaga aaacactgga
       61 agcaattcca caagtactcg agatagcaaa aaagatatct gtagagcggg tacgcgatga
      121 aataaagaaa atgcttgaag cggataaacc atctgaggga tttgagtata tgcgtgttac
      181 aggattacta gatctttaca tgcctgagct atcgcaaggg tttggaatgg aacagaataa
      241 ataccacatg tacgatattt attatcacag catctattcc tgtgatgctg caccaaagga
      301 aaaaccattg gtccgccttg cagcattgct gcatgatatt ggtaaaatcc ctacacggcg
      361 tgaaggcagt gatggtgatt atacctttta caatcatgag gtgataagtt ccaaaattgc
      421 acgcaagatc atgcgcaggc ttaaattttc caatgatgaa atagagtatg tcaccaatct
      481 tattgcaaac cacatgtttc attatacaaa tgactggaca gatggagcag tccgccggtt
      541 tatacgaaag gttgggttag ataatcttga tgatctattc ctattgcgcc ttgctgacag
      601 atgtggcaat ggaatgagag atggattgcc tgaacctatc aaaaagctta aacgacatat
      661 tcaaagagta atagaaaagg ataatgcctt tactgttaag gacttagata tagatggata
      721 tattttgatg gaacatctta aattgaaacc cgggccaata ataggcaaaa ttcttaatga
      781 gttactggaa caagtacttg acaaccctga attgaataat caggaaacac ttctgcaaat
      841 ggcacacgaa ttgtatgctg ccatggcaaa tgataccaca gccacttgcc atgacagcac
      901 ccagcatcat attgtttaag aaaagtaatt aggtaatatt gcttttagtt ttttcttttg
      961 tttgctgctt tgctttttgg gatcggtgat aattgcatat ttaattgctt ccttgcatcc
     1021 acaatccctt tctttagtaa gcaatggtat taattctttt ataagtgctt tggnnnnnnn
     1081 nnngtataaa atcgaatgaa ttatcggtag aacctctggc attcgaagca tttttattaa
     1141 gattattaac aaccatatca acagttacat gctcctcatc ttccttccag caatcataat
     1201 cagtgctcat gcagattact gcataacaca tctcagcttc gcgggcaagc ttggcttcag
     1261 gtaatgtact catgttgatc actcctgcct gccatgagcg gtagaggtgg ctttcagcac
     1321 gggtagaaaa tgctggtcct tccatgcaaa caagggtttc attagtatgc atgggtatgt
     1381 tcagtttttg tgctgcaaca agtattaatt cgtgaagtct agtgcagaat gggtcagcaa
     1441 aggcaatatg tgctgcaatt ccatcaccaa aaaaggttga aatgcgtgat tttgtacgat
     1501 caataatctg gttaggtatt acaaaatcaa gtggcttgat gcgttctttt aagctgccta
     1561 ctgcagaaaa tgccacaatc tggcctgcac ccagcatttt gagtgatgca atgtttgctc
     1621 gatagttaat ctcatgaggc aggataaaat gtccacggcc atggcgaggt aaaaatccag
     1681 ctttaactcc gccaacttct gcaatggtaa tagcatctga aggcatgccc cagggtgttc
     1741 ttacctttac ttcttccaga atagtgaccc cttcaatctg atacaggccc gagcctccaa
     1801 ttatgccaac atcaattttt ggtttcatag gttttctccc ttgaaaaaag tatctataat
     1861 caatttcatt ctccaggtat tattgatgtg atgctattgt aaataacaat gatttaattt
     1921 cttttatgtc aaatggcttt ttaatgtagc caaatgcctg ttctttgtca actaactctt
     1981 taatctggtc ttcataaaaa ataccggtga taaatacaac cttctgactg ggattttcat
     2041 cttttatcat tttgtatgca tctatgccag aaagtacagg catggataca tccataaata
     2101 caatatcata ctgattttct ttgcacaggg agtaaccttc atagccatta gttgcaaatg
     2161 taatagcgga tatgcctaat gattgtagaa gtgttttaaa aagatcacgg attgcatcgt
     2221 cgtcatcgac tattaacacg gtaaggttgc ttaaatcttt tggttgcgaa gaagcctcca
     2281 actcctttgg tagatatgcg gttttatata taggaagttc
//
LOCUS       DAOSZP010000373         2316 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_35450_length_2316_cov_3.743919, whole genome shotgun sequence.
ACCESSION   DAOSZP010000373 DAOSZP010000000
VERSION     DAOSZP010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2316
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35450_length_2316_cov_3.743919"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1086
                     /gene="pnp"
                     /locus_tag="PK348_08175"
     CDS             <1..1086
                     /gene="pnp"
                     /locus_tag="PK348_08175"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012283353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HXK66228.1"
                     /translation="HGSALFTRGQTQSLGIVTLGTVADSQRIDSIDLIEESYKRFMLH
                     YYFPPFSVGEVGRVGGVGRREIGHGMLAERALEYAIPDEDVFPYTIRVVSEILESNGS
                     SSMATVCSGSLALFNAGVPMKAPVAGIAMGLVLEGDRYAILSDIQGIEDHLGDMDFKV
                     AGTATGITAFQMDIKIEGITTEIMRKALEQAKEGRLYILEKMNEVISEPAKELSPHAP
                     KIAVMKIEIEKIGEVIGPGGRVIKKIIEESGADINIEPDGTIFISASEQDAIDKAQLS
                     IKGLVEEVEVGAIYKGIVKRITDFGAFVEILPGKQGLVHISKLDHSRVEKVKDILNEG
                     DEVLVKVIGIDEQGRINLSRKDAMKKH"
     gene            1095..>2316
                     /locus_tag="PK348_08180"
     CDS             1095..>2316
                     /locus_tag="PK348_08180"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HXK66229.1"
                     /translation="MVYKFTLPNGLTILLEPIDNVVSVSCGLWIKVGSRHEKANEMGY
                     AHFIEHMLFKGTDKYSAKDIARLVDRVGGQHNAATNREYTCYYINVVSDYLELAVEIL
                     SDTYLHSTFDEEEIEKEKNVVIEEIRMYEDTPDEHIHDLFMDTMFYNSPLGHSILGSF
                     NTISSINRESIVSFYKHHYTDNESLFVISGNFNIEEARQIIEKYFCKDCTATNNISIP
                     SLPKYERKLRVHLDKDLEQVHLCIGTDGLSKVDEDRWAYYLFNTILGGSMSSRLFQNI
                     REKEGLCYSIYCFHSSFMDTGVFGIYCGTAPSQYNKLLQLIANECRLITSQGISEEEL
                     ADAKSYLIGNMALSLESTEVRMSHIAKNEIVFGQQFTFKEIVRNIQAVTMEDFRRITH
                     RILDNAQFSLVTIGK"
BASE COUNT          700 a          377 c          558 g          681 t
ORIGIN      
        1 catggttcag cactgtttac tcgtggacag acacagagtt tggggatagt aaccttggga
       61 acagttgctg attcccagcg tattgatagt attgatttaa ttgaggaatc atataagcgg
      121 tttatgcttc actactactt ccctcctttc agtgttggcg aagttggaag ggttggcggt
      181 gtgggtcgtc gtgagattgg ccatggtatg ttagctgaac gagcactgga atatgctatt
      241 cctgatgaag atgtatttcc ctataccatc cgtgttgttt cagaaatact tgaatctaat
      301 ggttcttcat caatggccac tgtgtgttca ggttcgctgg cattgttcaa cgctggtgtg
      361 cccatgaaag ctccggttgc cggtattgca atgggtctgg tacttgaggg cgaccgctat
      421 gccattttaa gtgatatcca gggtattgaa gaccatctgg gcgatatgga ttttaaagtt
      481 gccggtactg caaccggtat tactgcattt cagatggata taaagattga gggtattacc
      541 acagagataa tgcgcaaagc attggagcag gcaaaagaag gcaggctcta tatactggaa
      601 aagatgaatg aagtcatatc agaacctgca aaggaactat cgcctcatgc accaaaaatt
      661 gccgttatga agatcgaaat tgaaaagatt ggtgaggtga ttgggcccgg cggcagagtc
      721 atcaagaaga tcatagaaga atcaggtgcc gatatcaata ttgagcctga tggtaccata
      781 ttcatttctg ccagtgaaca ggatgcaatt gacaaggcac agctttctat taaaggattg
      841 gttgaagagg tggaagttgg tgctatctac aaaggtattg taaagcgaat tactgatttt
      901 ggagcgtttg tagaaatttt gcctggcaaa caggggctgg tacatatttc aaagcttgat
      961 cactcgcggg ttgaaaaggt gaaggatatt ttgaacgaag gtgatgaggt gctggtcaag
     1021 gtgattggaa ttgatgagca ggggcgcatc aacttgagcc gcaaagacgc tatgaagaag
     1081 cattagtccg aaaaatggta tataaattta cacttcctaa tgggcttaca attttgcttg
     1141 agccaattga taatgttgta tcggtgtcgt gtggcctgtg gattaaggtg ggctcgcggc
     1201 acgaaaaagc caatgaaatg gggtatgccc atttcattga gcatatgctt ttcaaaggca
     1261 cagataaata cagtgcaaag gatattgcac gtctggtaga cagggttggc gggcagcaca
     1321 atgcagctac caaccgggag tatacctgtt attatattaa tgtggtatcg gattatttag
     1381 aattagctgt tgaaatactg tcagatacct atcttcattc cacatttgat gaagaagaga
     1441 tagagaaaga aaaaaatgtt gtaattgaag aaatccgtat gtatgaagat acaccggatg
     1501 aacacatcca tgatcttttt atggatacca tgttttacaa cagcccattg gggcattcaa
     1561 tactgggcag ctttaataca attagttcca ttaatcgaga aagtattgtt tcattttata
     1621 aacatcatta tactgataac gaaagtcttt ttgttatttc aggtaacttc aatatagaag
     1681 aagcacggca aattattgaa aagtatttct gtaaggattg taccgcaaca aataatataa
     1741 gcattccttc actaccaaag tatgaacgca aattacgcgt acacctggat aaagacttgg
     1801 agcaggtaca cctgtgtatt ggtactgatg gcttgagtaa agttgatgaa gacaggtggg
     1861 catattattt atttaatact attttaggcg ggagtatgtc atcgcgtctt tttcagaata
     1921 tacgtgaaaa agaaggattg tgttacagta tttattgttt tcattcatcg ttcatggata
     1981 cgggtgtgtt tggcatttat tgcggtacag ccccttcaca gtataacaaa ctattacaat
     2041 taattgcaaa tgagtgccgg ttaattacgt ctcagggaat ttccgaagag gagttggctg
     2101 atgcaaaaag ctaccttatt gggaatatgg cattaagtct tgagagtact gaggttcgca
     2161 tgagtcatat tgctaaaaat gaaattgttt ttggtcagca atttaccttc aaagaaatag
     2221 taagaaatat acaggcagta acaatggaag attttagacg cattactcac agaattttag
     2281 ataatgcaca gttttccctg gtgacaatag gcaaat
//
LOCUS       DAOSZP010000374         2304 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_35699_length_2304_cov_2.251223, whole genome shotgun sequence.
ACCESSION   DAOSZP010000374 DAOSZP010000000
VERSION     DAOSZP010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2304
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35699_length_2304_cov_2.251223"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1450
                     /gene="lnt"
                     /locus_tag="PK348_08185"
     CDS             <1..1450
                     /gene="lnt"
                     /locus_tag="PK348_08185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HXK66230.1"
                     /translation="VYIRNKPLKDVYITSFITGLFGFGVVYYWMGDFGQALPYGKVVI
                     VALIVPCISVFFATKVLLAEYLSRMFPRLTMILYPSVWIAFDWVQSIGTMAFPWNFLG
                     YTQYPFIHFIQIASITGIFGITFIIVFANISIAELIQAKLCNSNYRPALLACSIVCVV
                     VMVSTGYGFIRLKHHHEYANSHLRVAMVQSCFSPWENWTRNKYIYLDILKTLTQQAMQ
                     HDPDIVVWSESATLENISYSYFNGNLNHFEEEVLALAAANRVPLLTGEIGIIEDVLKR
                     RYYPQNSMVLINSAGQVVDTYAKIHLVPFGEWFPYGELFPFVNDIIDAFGGSNFIPGD
                     SPRIFTIGSFRCAPLICYENIFHRLCRYYANAGIDFYVNITNLLWTENPVGPMQHFYF
                     SVFRAIENGIWFVSAGNSGFTALIDPYGRITTSVPLMKKTYCVGDMDLSMNTGTVYRL
                     CGDIILYVAWVFILVLCAIVTANKLNAYKRKR"
     gene            1450..>2304
                     /locus_tag="PK348_08190"
     CDS             1450..>2304
                     /locus_tag="PK348_08190"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HXK66231.1"
                     /translation="MRIIYCTDVHGAFERVRELLYETIADVYIISGDLIDIPFYNMDS
                     AIRYHELQMYFDNMRKQMGRDDVTVEDFVDELMDMPHITEETAELGAKYQQYTIRARR
                     VMQQKYKVLANIFSFKPQTYIFALPGNYDMDLKYTSLHDRDLHLHWHQVDDQIIAGYG
                     GADVFTAGIPQKYVVPYRAGIGIDDRKNEMYNFFKAVRPTIIVTHQPAHGVLDWLPPV
                     GPTGSPALRTYCDNNPVILCLTGHIHGSWGMKEVEHTLYLNPSPFGDITTVHHDVLEG
                     GFFYQVELD"
BASE COUNT          621 a          429 c          527 g          727 t
ORIGIN      
        1 tgtgtacata cgtaataagc cgctgaaaga cgtttacata acatcattca ttacaggact
       61 ttttgggttt ggtgttgttt actactggat gggtgatttt ggacaggcgt tgccgtatgg
      121 caaggttgta attgtagccc ttattgttcc gtgcatatcg gtcttttttg ctacaaaggt
      181 tttacttgct gaatatctgt cgcgaatgtt tccacgtctt actatgatac tgtatccatc
      241 ggtgtggatt gcatttgact gggtacagtc cattggtacc atggcatttc cctggaactt
      301 tttaggttat acgcaatatc cgtttattca cttcattcag atagcgtcaa ttactggaat
      361 ctttggtatc acgtttatca ttgtgtttgc aaatatcagc attgctgaat taatacaggc
      421 aaagctttgt aacagtaact atcgccctgc attacttgcg tgcagtattg tatgtgttgt
      481 tgtgatggta tcaacgggtt atggtttcat aagactcaag caccatcatg agtatgcaaa
      541 ttcacacctg cgtgttgcca tggtgcagtc atgtttttcg ccatgggaaa actggacaag
      601 aaataaatat atttatctgg atattcttaa aacgttaaca cagcaggcta tgcagcatga
      661 tcctgatatt gtggtgtggt cagaatccgc aacgttagag aatatttcat atagctactt
      721 caatggcaat ctgaatcatt ttgaagaaga agtgctggcg ctggctgcag ccaaccgtgt
      781 acccctgcta acaggtgaaa ttggcattat tgaagatgtg ctaaaaaggc ggtattatcc
      841 gcaaaacagc atggtgctta tcaactcggc aggtcaggtt gttgacacat acgccaaaat
      901 acatttggtg ccctttggcg agtggtttcc atacggggaa ctgttcccgt ttgtgaatga
      961 catcattgat gcatttggtg gttcaaactt tattcctggt gatagcccgc gtatttttac
     1021 cattggttca ttcagatgtg ccccacttat atgctatgaa aatatttttc acagattatg
     1081 caggtattat gcaaatgcag gcattgactt ttatgtaaac atcactaatc ttttatggac
     1141 cgaaaatcca gttggtccca tgcagcattt ttacttttca gtattcagag caattgaaaa
     1201 tggcatatgg tttgtcagtg cgggcaattc aggttttacg gcactcatag atccgtatgg
     1261 gcgtattact acttcagtgc cgctcatgaa aaaaacatac tgtgtgggtg atatggattt
     1321 gagtatgaat acaggcacag tgtatcgcct gtgtggtgat attatcctgt atgttgcatg
     1381 ggtttttatt cttgtattgt gtgcgatagt tactgctaat aagcttaatg catataaaag
     1441 gaaacgctga tgcgtataat ctactgtacc gatgtccatg gcgcgtttga acgcgtacga
     1501 gaactattgt atgaaacaat agccgatgtg tatatcatct ctggtgattt gatagacatt
     1561 ccattttaca atatggacag cgcaattcgc taccatgagt tgcagatgta ttttgacaat
     1621 atgcgtaagc aaatgggacg tgatgatgtc acggtggaag attttgttga tgagcttatg
     1681 gatatgccgc atatcacgga agagacagca gaactgggtg caaaatatca gcagtacacc
     1741 atacgtgcac gcagagtgat gcagcaaaag tataaggtgt tggcaaatat ttttagcttt
     1801 aaaccgcaaa catatatttt tgcactgcct ggcaattacg atatggattt aaaatacaca
     1861 tcgcttcacg acagggacct gcatctgcac tggcatcagg tcgatgatca gattattgct
     1921 ggctatggtg gggctgatgt attcacggca ggtataccac aaaagtatgt tgtgccctat
     1981 agagctggca ttggtattga tgatagaaaa aacgagatgt acaatttttt caaagcagta
     2041 cgaccaacta tcattgtgac acatcagcct gcccatggag tgcttgactg gctgccaccc
     2101 gttggtccaa cagggtcacc tgcgctcaga acctattgcg acaataatcc tgttatttta
     2161 tgtttaactg gccacattca cggatcatgg ggtatgaagg aagttgaaca tacgttgtat
     2221 ttaaatccat cgccatttgg ggacattacc accgtgcacc atgatgtgct tgagggaggg
     2281 tttttttatc aggtagaact tgat
//
LOCUS       DAOSZP010000375         2300 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_35764_length_2300_cov_3.440980, whole genome shotgun sequence.
ACCESSION   DAOSZP010000375 DAOSZP010000000
VERSION     DAOSZP010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2300
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35764_length_2300_cov_3.440980"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(70..1011)
                     /locus_tag="PK348_08195"
     CDS             complement(70..1011)
                     /locus_tag="PK348_08195"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HXK66232.1"
                     /translation="MDIQLPLSVRIRLLLKRYLEALNMERRVVVPRYEPDYKPQWGVI
                     IVLFATTFLTTSIAGSSGGDTLLEIIINGLPFSISLLGILMAHEMGHYFAARYFKVIA
                     TPPYFIPFPSIVGTMGAVIRIKSPIPDKRALLYIGAMGPIAGFIISCIISILGLYQST
                     VQPLPQPAPDAIVPVFGDSLLYYLFTKLIHGTIPPGSDVHLSPLAWAGWIGFLVTNLN
                     LMPIGQLDGSHVIYALFGERQRYAGWVFFVILFFLAILWPGWAVWIILTLTLLMIGHP
                     FIPDGRPLNTWERCIGYMCVIIFILTFIPVPVSILGQ"
     gene            complement(1014..>2300)
                     /locus_tag="PK348_08200"
     CDS             complement(1014..>2300)
                     /locus_tag="PK348_08200"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HXK66233.1"
                     /translation="HGHVLATQPRLKGNRVGIITNSGGPGTSMANTCEKEGFTVPPFS
                     RQLQEKIKPMIPPHAPAGNPVDITFNLDLDVLTIKIPELAIQSGEVDALCIHGAFRKG
                     FIECIFPHVQQALHLDKPEDVLGFLPSDLDTPGTIPYKYGIPIVMSSFFDYQDDFTVA
                     MQEKGVPVFDSPEKAAKALAVLLRYKEVTQRLPYEKVPLPDVNPQASSIIQKALKNKQ
                     KALDEHAAKTLLALYGIPIPDETLIESKKELSAIVDSLKYPVVMKACHPDIMHKTEKG
                     LVILPVKDSNEAKVAFNEIQKRAGFATPVLVYTMVEGKREFMAGAIHQKGFGKAVLFG
                     LGGIFTEALKDITFRVAPLTKRDAFEMLYDIHAKTLLGKFRNEEEVDTSSLANLLCIL
                     STVPLLHPEISEIDCNPIIISGSKPVVIDALVVLNN"
BASE COUNT          731 a          486 c          401 g          682 t
ORIGIN      
        1 tcacctatta ctggtttcct gccattaatc tcatacagtg gcatgtatac ctccaaatat
       61 tgaatataat tattgcccta aaatacttac cggaaccggt ataaacgtaa ggataaaaat
      121 aatcacacac atatacccta tacaccgttc ccacgtattc aatggcctac catcaggaat
      181 aaacggatgc cctatcataa gcaatgtaag ggtaaggata atccatactg cccatcctgg
      241 ccacaatatt gctaaaaaga ataatatcac aaaaaagacc cacccagcat atcgttgcct
      301 ttcaccaaac aatgcataga taacatggct accatcaagt tgaccaatag gcataaggtt
      361 taaattggta accagaaaac caatccagcc agcccatgca agaggcgaaa gatgaacatc
      421 gctaccagga ggaatggtac catgaatcaa ttttgtaaaa agatagtaca gcaatgaatc
      481 accaaatact ggaactattg catcaggtgc tggttgtgga agaggttgta ctgtagactg
      541 atacagccct agtatagaaa ttatacaact tattataaaa ccagcaatgg gccccattgc
      601 acctatatat aacaatgcac gcttgtctgg tattggcgat ttaatgcgaa taacagcccc
      661 cattgtgcct acaatggaag ggaaaggaat gaaatatggt ggtgttgcaa taaccttaaa
      721 atagcgtgca gcaaaataat gccccatttc atgagccatt aaaatcccta aaagagaaat
      781 tgaaaatggt aagccattaa taattatttc taaaagtgta tcacctcccg aagagccagc
      841 aattgatgtt gttaaaaatg ttgtagcaaa aagtacaatt ataacacccc attgtggttt
      901 gtaatcaggt tcatagcgag gaaccaccac tctgcgttcc atattaagcg cttctaaata
      961 gcgtttcaat aataatcgta tccgcactga taatggcaat tgtatatcca tagttaatta
     1021 ttcaatacca ccagagcatc aataacaact ggcttacttc cactgataat tattgggttg
     1081 caatctattt ctgaaatttc tgggtgcaat aatggtacag tactcaatat gcacagaaga
     1141 ttagccaatg aagatgtatc cacttcttct tcattcctga atttgcctaa cagtgtttta
     1201 gcgtgtatat catagagcat ctcaaatgca tcccgttttg taagtggagc cactctgaac
     1261 gtaatatctt tcaatgcttc cgtgaaaatt cctcctaatc caaaaagaac tgctttgcca
     1321 aatcctttct gatgtattgc tcctgccatg aattcccgtt tgccttcaac catagtatat
     1381 actaaaaccg gagttgcaaa ccctgcacgc ttttgtattt cattaaatgc aacttttgcc
     1441 tcattactat ctttaacagg aagaattacc aacccctttt cagtcttatg cattatatca
     1501 ggatgacatg ctttcattac aacgggatat ttcaatgaat caacaatagc acttaattct
     1561 tttttagatt caataagtgt ttcatcagga atagggatac catataaggc taacagtgtt
     1621 tttgcagcat gttcatccag tgctttttgt ttattcttta atgctttctg aatgattgaa
     1681 gaagcctgag gattgacatc tggtaaggga accttttcat aggggagccg ctgtgttact
     1741 tctttatatc gtaataatac tgccagtgct tttgctgctt tttcagggct gtcaaataca
     1801 ggcacacctt tttcctgcat tgcaacagta aaatcatcct gataatcaaa aaacgatgac
     1861 ataacaatcg gaataccata cttatagggg atagtacctg gagtatccaa atcacttggt
     1921 aaaaacccca acacatcttc aggtttatct aaatgcaatg cctgctgcac atgagggaat
     1981 atacattcta tgaaaccttt tctgaatgca ccatgaatac atagggcatc aacctctcca
     2041 ctctgtattg caagctctgg tattttaatt gtcagcacat ctaaatccag gttaaatgta
     2101 atatctacag gattccctgc aggtgcatgt ggtggtatca ttggttttat tttttcctgc
     2161 aactgacggc tgaatggagg tactgtaaaa ccttcttttt cacaggtgtt ggccattgat
     2221 gtccccggac ctcctgagtt tgtaataata ccaacacgat tgcctttaag ccttggctgt
     2281 gttgcaagca catgaccatg
//
LOCUS       DAOSZP010000376         2298 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_35789_length_2298_cov_3.670531, whole genome shotgun sequence.
ACCESSION   DAOSZP010000376 DAOSZP010000000
VERSION     DAOSZP010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2298
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35789_length_2298_cov_3.670531"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(620..1291)
                     /locus_tag="PK348_08205"
     CDS             complement(620..1291)
                     /locus_tag="PK348_08205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66234.1"
                     /translation="MIVDKTSSDAYFKTMEEMKKTKIKSYWDLLSVLKEHPDWLEELR
                     KLILTEELLALPKKFDEFVKRYESFIENEFKPLKATVEKQGNDVGSLKGDNFERKVKE
                     KAPSYFGKLILKCRLIDSMELANSIDDAILNKKATITDDERDDALKVDVVVTGFLRHN
                     KEKKVFLAAEVSIIVDKTDVERAARRTKIIEKCLGIPGIPVVIGDEYTDGAKKAAEEL
                     QVIMI"
     gene            complement(1327..2064)
                     /locus_tag="PK348_08210"
     CDS             complement(1327..2064)
                     /locus_tag="PK348_08210"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXK66235.1"
                     /translation="MALLQVNQVSVRYDERKVIDAVSFEVQESTITALTGKSGSGKTT
                     LLGVISGLLQPNEGTVLFMGDNIFTWSDLKRSRFRNRDIGFVFQFFNLFSDISAYDNI
                     AFPAILNRETRNLHARIMELAEYLQITDILDRNPTTLSGGERQRVAIARAIINEPKLI
                     LADEPTGNLDTATANDILKLFARLRDDKGISIIIATHDERIVKASNVQYHLEGGKILE
                     NGVRKEEHKIVKKKPITRPAAKIKKKK"
     gene            complement(2074..>2298)
                     /locus_tag="PK348_08215"
     CDS             complement(2074..>2298)
                     /locus_tag="PK348_08215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66236.1"
                     /translation="YGFIGYLAGAYLIKAAGAILSSLVPALSGIGLEPVGMNVCAGLV
                     LFSIAISSLSALIPAIFASNMNLFKALSKQ"
BASE COUNT          673 a          500 c          351 g          774 t
ORIGIN      
        1 ttggcgatag ttactatgta agggattgtt ttattggaac aaggctcaat aagcatatca
       61 gcaggtccac caaccttaaa ggtggtaaat tgactcatag gaacatcaac gtacagcctg
      121 ccatactgcg acagttcctt tataacatta tgtgaacagg tttgtcccaa tggtatttat
      181 ctcctctgga aataggattt tatataatag atgtcattgc aatcccaata taatcagtta
      241 tgtctcgata caattactat acaatcactc gacgaccgca tcttgataga tccttcgctc
      301 cgactttatc gggactcagg ataaaaaata cccaggtcat tctgaggacg ccccaccttt
      361 attggggcgg acgaagaatc ttgccgtttc cgttttacac acagtcattg ctatttacat
      421 gagactgttt accttcaatt cagttcagag caatcacttt atttagtaat gaaaatattt
      481 gcatcatcaa caaaagttac catcaccgca tctacaacca tggacaacgt ttatataaca
      541 aatattaaat accaccttca ttgatatttt gcgatgaaaa tcaacaatat ttttaaaaaa
      601 ttaactatga ggaggaatgt tatatcatta tcacctgcaa ttcttcagca gctttctttg
      661 ctccatcagt atattcatca ccaattacca ccggtattcc aggtattcct aaacactttt
      721 caatgatttt tgttcttcta gctgctcttt caacatcggt tttatccaca ataattgaaa
      781 cctcagcagc caaaaaaacc tttttttctt tattgtgacg caaaaaacca gtaacaacca
      841 catcaacttt cagtgcatca tcacgttcat catcagttat agtggctttt ttgttcagta
      901 tagcatcatc aatactattt gccaattcca tgctatcaat taatctgcat tttaaaatga
      961 gttttccaaa atacgaaggt gctttttctt ttaccttccg ttcaaaattg tctcctttta
     1021 ggcttccaac atcattaccc tgtttttcaa cagttgcttt taagggctta aattcatttt
     1081 ctataaagct ttcatagcgt tttacaaatt catcaaattt tttaggcagt gcaagcagtt
     1141 cctcagtaag aataagtttc ctgagttctt ctaaccaatc aggatgttcc ttcaaaactg
     1201 aaagtaaatc ccaatacgac ttaatctttg tttttttcat ctcttccatg gttttaaaat
     1261 aagcatccga tgatgttttg tcaactatca tatacttctc tcctggtaca catgtatctt
     1321 tatttattac tttttctttt taatttttgc tgcaggcctt gttataggct tttttttcac
     1381 tattttatgc tcttcttttc taaccccatt ttccagaatt tttccacctt caagatgata
     1441 ctgcacattg cttgccttta caatacgttc atcatgggtt gctattatta ttgatatccc
     1501 cttgtcatca cgcaagcgcg caaatagctt caatatatca ttggctgtgg cagtatccaa
     1561 gttgccggtt ggctcatctg caagtattaa ttttggttca ttaatgattg cccgcgctat
     1621 tgccacacgc tggcgttcac cacctgacaa tgtggtagga tttctgtcca gtatatcggt
     1681 aatctgcagg tattctgcca gttccattat acgcgcatgt aaattacgcg tttcccggtt
     1741 aagaattgcc gggaatgcaa tattgtcata cgctgaaata tcagaaaaaa gattaaaaaa
     1801 ctggaagaca aagccaatat ccctgtttct gaaacgcgac cgctttaagt cactccatgt
     1861 aaaaatatta tcacccataa aaagtactgt gccttcattg ggctgcaata atcctgatat
     1921 tactcccaga agagttgttt tccctgaacc tgatttacct gttaatgcag tgatagtgct
     1981 ttcctgaact tcaaagctga ctgcatcaat aactttgcgt tcatcatacc tgacacttac
     2041 ttggtttacc tgtaataaag ccacggtttt acctcactgt ttgcttaatg ctttaaataa
     2101 attcatattt gatgcaaaaa tagcaggtat caatgctgac aatgatgaaa ttgcaattga
     2161 gaaaagaaca agaccagcac atacattcat gcctactggc tcaaggccaa taccgcttaa
     2221 tgccggcacc agtgatgaaa gtattgctcc agcagccttt attaagtatg cacctgctag
     2281 atacccaatg aaaccata
//
LOCUS       DAOSZP010000377         2288 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_35984_length_2288_cov_3.625168, whole genome shotgun sequence.
ACCESSION   DAOSZP010000377 DAOSZP010000000
VERSION     DAOSZP010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2288
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35984_length_2288_cov_3.625168"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            19..202
                     /gene="ssrS"
                     /locus_tag="PK348_08220"
     ncRNA           19..202
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PK348_08220"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            221..1264
                     /locus_tag="PK348_08225"
     CDS             221..1264
                     /locus_tag="PK348_08225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66237.1"
                     /translation="MEIITVFGVRWRVAPLWEVGLTVLVILLVIAFFALLKYNQYLKL
                     KKSSLEQIFIYKARQRGLTGYQVRILQHIVALSKLSSPEIIFINKALFETGLVKFLQY
                     MAKLKEPPDDLKDIFKDIVITYERIYNPVEYQQPLDSIEDISADTLVCGYSQTGRAFI
                     AKIMLIAEDTIHCRYFRKPGQLPDEIITEPVSIFFFRNGDAEYSFESEITAIQENTIL
                     LKKPDAIKRGKEVHHPYIQTLIPCTIQKKKKVDKEEEEAIIQHDDNEPVNGTIERLNE
                     FEAVVRVEKTLQYQNEYTLFFTLENFKIEIVSKILASRTITKEKIIYYTFKLIEMSEA
                     AQNVISKYVKERL"
     gene            complement(1284..2288)
                     /gene="dnaJ"
                     /locus_tag="PK348_08230"
                     /pseudo
     CDS             complement(1284..2288)
                     /gene="dnaJ"
                     /locus_tag="PK348_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463625.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
BASE COUNT          720 a          489 c          390 g          689 t
ORIGIN      
        1 ctttgtgtat attagattat aagcctgcgg tgttcgtgta tggtatgtca tattgtagtt
       61 ccaacactac tgagcatgga atctctaatc tgcaggttga tatcatgctt ctttacgaag
      121 tagatagata cctgtatgcg ggaatccacc tttaccaagg gagctcaaat attcatacca
      181 ctacggcaca cgcgggttac tataaaacat aggcaaaaat atggaaataa ttacagtatt
      241 tggagttcgc tggcgtgttg ctcctttatg ggaagttggc ttaacggtat tggtaatcct
      301 tttagtaata gctttctttg cattgctaaa atataatcaa tatctcaaac ttaaaaaatc
      361 cagcttagag caaattttta tctataaagc aaggcagcgt gggcttaccg gttaccaggt
      421 tcgcatactt cagcatattg ttgcactatc caaactgagc tcgcctgaaa ttatatttat
      481 caataaagct ttatttgaaa ccggtttagt taagtttctg caatatatgg ccaaactaaa
      541 agaacctcca gacgatttaa aagatatctt caaggatatt gttattacgt atgaacgaat
      601 ttataaccct gtagaatatc agcaaccact tgattcaatt gaggatatat ctgcagatac
      661 actggtttgt ggatacagcc agactggtag agcatttata gcaaaaatca tgctaattgc
      721 agaagataca atccattgca ggtatttcag aaagccaggg caacttcccg atgaaataat
      781 aactgaacct gtatcaatat tcttttttag aaatggtgat gctgaatatt cctttgaatc
      841 tgagattaca gcaattcaag aaaacacaat actattaaaa aaacctgatg caataaaacg
      901 gggaaaagag gttcaccacc catatataca gacacttatt ccatgcacta ttcaaaagaa
      961 aaaaaaagta gataaagaag aggaagaagc aatcatacaa catgatgaca atgagccagt
     1021 gaatggcact attgaacggc ttaatgaatt tgaagcagtt gtgcgagtag aaaaaacatt
     1081 acagtaccaa aatgaatata ccctgttttt tactctggaa aattttaaaa ttgagatagt
     1141 cagcaaaatt ttagcttctc gcactataac aaaagaaaag attatctatt atacatttaa
     1201 acttattgag atgtctgaag ctgcacaaaa tgttatatca aaatatgtaa aagagcggct
     1261 gtaacaaacc gctcttttat tatttatgta taaaactcac ggtctgaatc aacagtttca
     1321 ccttcaatac gggcaaactc ctttaatagc tccttctggc gcggactgag tttctttggt
     1381 actttaatgt taatctttac cagctgatct cctttgccat acgatcctaa atacggcatg
     1441 ccattaccct taagtctgaa aatctggcca ttctcagtgc ctgcaggtat tttcatcttg
     1501 gctttttttc catagattgt tggcacttct atctcaccac ccaaacaggc aacagcaaaa
     1561 gaaacatcca caatgcttat caggtcatta ccatgacgtt caaacattgg atgtttttga
     1621 atatgaacca caacatacag atctccagca ggccctccgc ctggtccctg ctcaccttca
     1681 cctgcaatct tgaggcggga acctgattca acgccagccg gtatttttac tgtaatcttg
     1741 cgatgttttt cctcaagacc tgttcctttg cataccctgc atggcgaagt tataatcttg
     1801 ccttcacccc tgcatttata acaggtctga gtaattgaga aaaacccctg tgtctggcgt
     1861 atctgtcctg taccattaca tacgggacat actgttggtt ttgttcccga cgctgaacct
     1921 gttccaccac atgcactgca aacttcatga cgcggaattt ctatctgaac ttctttacct
     1981 aacgctgcat cttcaagcgt aattgaaagg tcatattgaa tatccctacc acgtctgaca
     2041 cgtgttgaac gggtcctggt accaaaacca gcaccaaaaa aactttcaaa tatatcccct
     2101 aaatcaaaat caccaaagat atccgaaaaa tctgtatatg cccctctgcc aaaaccttca
     2161 aatccagcaa caccttaatg tccaaattta tcatactgcg cacgcctttt ggggtcacgc
     2221 aatacttcat aagcttcagc agcttctttg aatttttctt ctgcttcttt gcttccttta
     2281 ttgcgatc
//
LOCUS       DAOSZP010000378         2288 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_35990_length_2288_cov_2.653829, whole genome shotgun sequence.
ACCESSION   DAOSZP010000378 DAOSZP010000000
VERSION     DAOSZP010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2288
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35990_length_2288_cov_2.653829"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /locus_tag="PK348_08235"
     CDS             <1..277
                     /locus_tag="PK348_08235"
                     /inference="COORDINATES: protein motif:HMM:NF014009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HXK66238.1"
                     /translation="AAITTIEFEPGLQKDINLFLEKIIPRNAHYHHHDTWHDDNGSSH
                     LRASMIGPSITVPIVDGTLTLGTWQQIVVIECDTRKRNRKIVAQIVY"
     gene            430..1146
                     /gene="prxU"
                     /locus_tag="PK348_08240"
     CDS             430..1146
                     /gene="prxU"
                     /locus_tag="PK348_08240"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_083777221.1"
                     /note="Most members of this family contain a
                     selenocysteine; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_except=(pos:676..678,aa:Sec)
                     /transl_table=11
                     /product="thioredoxin-dependent peroxiredoxin"
                     /protein_id="HXK66239.1"
                     /translation="MPEEFKPGCTRPTTSPPPQDIILQEQQNSSKKEEHTMIQVGQKA
                     PEFSLPAYFKGKFVEVKLSEYLGKWVVLCFYPGDFTFVUATEVSAVAEKYPEFQKLGV
                     EVLSASVDSVFVHKMWNDNELSKMVKGGIPFPMLSDAGGKVGTMYGVYNSNAGVENRG
                     RFLIDPDGVIVGYEVLTPPVGRNVLETIRQIQAFQLVRESKGTQATPSGWKPGKDTLK
                     PGPDLVGKVWQVWTVDKAFD"
     gene            1242..2183
                     /locus_tag="PK348_08245"
     CDS             1242..2183
                     /locus_tag="PK348_08245"
                     /inference="COORDINATES: protein motif:HMM:NF013046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HXK66240.1"
                     /translation="MSYTAYCFHPHYLHHKTGDSHPESPKRLEILDKYIRTNKNYSSL
                     LYVQPRKADPAVLTYAHDADYVTMIASAKPNGITCIDGYDTVICDETYDVSLWAIGGI
                     LEVCDYILAGEATNGFCAIRPPGHHAEIAQAMGFCIFNNVAIVARYLQYTYNIRKIAI
                     IDWDAHHGNGTQHIFETDPSVFYISTHQFPFYPGTGSVDEIGIGPGYGYTINIPMNPG
                     SNDKTYKSAFTEIIIPALDHFKPEFILVSAGFDAHKSDPLSSLNLSSGAYYMFTRMLM
                     DIATRYSNNRIVVVLEGGYEIQSMIESVDYVLKALME"
BASE COUNT          741 a          416 c          445 g          686 t
ORIGIN      
        1 ggcagctatc accacaattg agtttgagcc gggactacag aaagatataa atctattttt
       61 agaaaaaata atcccacgta atgcacatta tcatcatcat gacacctggc acgatgataa
      121 tggttcttca cacctgagag cttctatgat tgggccatct attactgtac ctatagttga
      181 tggaacacta acgttaggaa cctggcagca gatagttgtc atagaatgcg atacccgcaa
      241 gagaaataga aaaatagtag cacaaatagt gtattaacaa aaatgtcaac atttttgaaa
      301 tattgagtaa cttacaaaaa gacaattttt tataagtttc ttcatattca tttgacataa
      361 ttatttttta tcattataat ttttacaaat atatattaat ttcaatcatc aatacagaag
      421 gaggtccgta tgccagaaga gtttaaacct ggatgtacac gtcccaccac ttcgccacca
      481 ccacaggaca tcattttgca agaacaacaa aattcatcaa aaaaggagga acacacaatg
      541 attcaagttg ggcaaaaagc accggaattt tctctgccag catattttaa agggaaattt
      601 gttgaagtaa aattatctga atatctgggt aaatgggtag tactctgttt ttacccggga
      661 gatttcacat ttgtctgagc tacagaagtt tctgcggtcg cagaaaaata tccagaattt
      721 caaaaattgg gggttgaagt tttatcagca agcgttgata gcgtttttgt acacaaaatg
      781 tggaacgata atgaattgtc aaaaatggta aaaggtggaa tacctttccc tatgctttct
      841 gatgcaggcg gtaaagttgg gacaatgtat ggagtttaca acagcaatgc tggcgttgaa
      901 aaccgtgggc gatttttaat agatccggac ggagtcatag ttggttatga agtgctgaca
      961 cctcctgttg gtcgtaatgt ccttgaaaca atacgtcaaa tacaggcttt ccagttagta
     1021 agagaaagta aaggtaccca ggcaacccca tcaggctgga aaccaggcaa ggataccttg
     1081 aagccagggc ctgatctggt tggcaaagtc tggcaggtgt ggactgtcga taaggcattt
     1141 gactaaaata gcgaaaaaat acttgatttt tttaaatatt atattacaat attatatagt
     1201 agtttgtttg tgttaattaa actatcaaac atttgataat tatgagctat acagcatatt
     1261 gctttcaccc gcattacctg caccataaaa cgggagattc acatcctgaa tctcccaaac
     1321 gtttagagat acttgataaa tatatccgta ccaataaaaa ttattcctca ttgctttacg
     1381 ttcagccacg taaagctgat cccgcagttt taacatatgc ccatgatgcc gattacgtta
     1441 caatgatcgc cagtgcaaag cctaatggaa taacctgtat tgatggctat gacactgtca
     1501 tatgcgatga aacgtatgat gtatcgttat gggcaattgg agggatactt gaggtatgtg
     1561 attatatcct tgctggtgaa gccaccaatg gattctgtgc tataagaccc cctggccatc
     1621 atgcagagat agctcaagca atgggcttct gtatttttaa taatgttgca atcgttgcac
     1681 gctatctcca atatacctat aatatacgaa aaatagccat aatagattgg gatgcccatc
     1741 atggcaatgg cacgcagcat atttttgaaa ccgacccttc tgttttttat atcagcacac
     1801 atcagttccc cttttatccc ggtacgggca gtgttgatga gataggcatt ggccctggat
     1861 acggatatac tataaacata cccatgaatc ccggtagcaa tgataaaaca tacaaaagtg
     1921 ctttcactga aattataatt cctgctttag accactttaa acctgaattt attcttgtat
     1981 cagcaggttt tgatgcccat aaaagtgacc cgctttcatc attaaatctt tcaagcgggg
     2041 catattacat gtttactcgc atgcttatgg atattgcaac acggtattcc aataacagga
     2101 ttgtggtggt gcttgaaggt ggatacgaaa tacaatcgat gatagaaagt gttgattatg
     2161 ttcttaaagc attaatggag taattacctg aaaataaatt cagcataatt atgatgtttt
     2221 gtagttctgc tggtaaaaat aattcccagt ggttttgaaa tttctaaaac atgatcaaag
     2281 acttttgc
//
LOCUS       DAOSZP010000379         2284 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_36060_length_2284_cov_4.897263, whole genome shotgun sequence.
ACCESSION   DAOSZP010000379 DAOSZP010000000
VERSION     DAOSZP010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2284
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36060_length_2284_cov_4.897263"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1234)
                     /locus_tag="PK348_08250"
     CDS             complement(<1..1234)
                     /locus_tag="PK348_08250"
                     /inference="COORDINATES: protein motif:HMM:NF014594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HXK66241.1"
                     /translation="MGDIEKWEYDQKKITADEAARFVKSGYRVFYGEFVLFPEAIDEE
                     LAKRANELHDVDVRSVCYMRVPKIVEADPEREHFILNDFHFGTISRRLYDQHLCNYIP
                     MTYHQGPRVAKKYLDFDIVFLTVGPMDPRGYFNYGLANSVTSAVVTKAKKIVVEVNEH
                     VPYCLGGNQESIHISRIDHIVEGKNLPLPQIPALEPTEIDKQIATHLLAEIEDGACLQ
                     LGIGGLPNIVGQMIAGGDFKDLGVHTEMLVDSYVDLYQAGRVTGARKNIDKYKMSYTF
                     AMGTDKLYDFLHHNPTCASYPVNYINDPRIIALNDKVVAVNNAIEVDLFSQVSSESVG
                     IVHKSGTGGQLDFIFGAFGSHGGKGFICLSSTYHNKNGNIVSRIVPTLPHGAIVTVPR
                     SIVHYVVTEYGAVQLKGKS"
     gene            complement(1989..>2284)
                     /locus_tag="PK348_08255"
     CDS             complement(1989..>2284)
                     /locus_tag="PK348_08255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66242.1"
                     /translation="LQLDTNKVKEILYTFDPLKAYSFPQVDTAMLKGKIASLTVAVQN
                     ENNEKAAEEIRLTVHKEYEDNRYICTCSKTPYIFTIDEWRAKRIFKTLQDLKK"
BASE COUNT          700 a          486 c          349 g          749 t
ORIGIN      
        1 ttgattttcc ttttaattgc actgctccat attcggtaac cacatagtgt actattgacc
       61 ttggaacagt aactatcgcc ccatggggca atgtaggaac aattcgtgaa actatattcc
      121 catttttatt atgataggta gatgacaaac aaataaatcc cttgccacca tgcgaaccaa
      181 atgcaccaaa tataaaatca agctgtcctc ccgtaccaga cttgtgaact attcccaccg
      241 attcagaaga tacctgactg aacaaatcaa cctcaatggc attgttaacc gctactacct
      301 tatcattgag tgcaataata cgaggatcgt ttatatagtt tacgggatat gaagcgcatg
      361 ttggattgtg atgcaaaaag tcatatagtt tatcagtacc cattgcaaag gtatagctca
      421 ttttatattt atcaatattt tttcgggcac cggtaaccct tcccgcctga tataaatcaa
      481 cgtatgaatc aacaagcatc tcagtgtgaa cacctaaatc cttaaaatca cctccggcaa
      541 tcatttgacc tactatattg ggcaatccac ctattcctaa ctgcaaacat gcaccgtctt
      601 caatttcggc cagaagatgc gttgcaatct gtttatcaat ttcagttggc tccaatgcag
      661 gtatctgcgg caatggtaaa ttttttcctt ccacaatatg gtcaatacgc gatatatgaa
      721 ttgattcctg attgccaccc agacagtatg gaacatgctc attaacttca acaacaattt
      781 tttttgcttt tgttacaaca gcggatgtaa ccgagtttgc cagaccataa ttaaaataac
      841 cccgcgggtc catagggcct accgttaaaa aaacaatatc aaaatcaaga tattttttgg
      901 ctactcgcgg tccctgatga taggtcattg gtatgtagtt acaaagatgc tgatcataca
      961 atcttcgtga gatagttcca aaatgaaagt catttaatat aaaatgttct cgctctgggt
     1021 cagcttcgac aatttttgga acacgcatgt aacatacgct acggacatca acatcatgca
     1081 attcgttagc tcgttttgca agctcttcat caatagcttc aggaaacaaa acaaattcac
     1141 cataaaaaac cctgtaaccc gatttaacaa aacgtgctgc ctcatctgca gttatctttt
     1201 tttgatcata ttcccatttt tcaatatcac ccattttgcc actcccctga taataaaatc
     1261 ttattattgt aagactacat atattaaata atattgttat ttaccataca acaccattgc
     1321 actatattga gtatagcatt aacaaattta taattcaaat tattatatat tattttatat
     1381 tatattttca tttttgttga ttaaatacca actggttagt ttatataaat aatattaatt
     1441 ctaagcactg taagtgttga atgtaaataa tgaaacgtat ctttttatat tatcaatata
     1501 agagaacgtt ggtacttttt tcattctggg gtaatcatca ctttttctca ttctgaggga
     1561 gcaaagcaac catgaatcta ttgttcataa aacgaaatga ttctttactt tgttcaggat
     1621 ggctattggc tttattctgg atgacaggtg ttactaaaaa gcaagattct ttgctaaagc
     1681 tttgctcagc acaggcattt tgttcagaat ggcaacagtg ttacatttcg catcccactt
     1741 gaaatggcaa aatccgttta tcgtagactc accccattac ttcttcaaat cctgcaatgt
     1801 cttgaaaata cgcttagccc tccattcatc aatgggtaac tatatacggt gggtattact
     1861 caataaccca ccactcaccc cattacttct tcaaatcctg caatgtcttg aaaatacgct
     1921 ttgccctcca ttcatcaatg ggtaactata tacggtgggt attactcaat aacccaccac
     1981 tcaccccatt acttctttaa atcctgcaat gtcttgaaaa tacgcttagc cctccattca
     2041 tcaatggtaa atatatatgg cgtttttgag catgtgcata tatagcgatt atcttcatac
     2101 tctttatgaa ctgtaaggcg tatctcttcg gcagcttttt cattgttttc attttgcact
     2161 gcaacggtaa gcgacgcaat tttacccttc agcattgcag tatcaacctg tgggaatgaa
     2221 tacgccttca atggatcaaa ggtatacagt atctctttta ctttattagt atccagttgc
     2281 aatg
//
LOCUS       DAOSZP010000380         2280 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_36141_length_2280_cov_3.791910, whole genome shotgun sequence.
ACCESSION   DAOSZP010000380 DAOSZP010000000
VERSION     DAOSZP010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2280
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36141_length_2280_cov_3.791910"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..920
                     /locus_tag="PK348_08260"
     CDS             <1..920
                     /locus_tag="PK348_08260"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXK66243.1"
                     /translation="FLISATREIVTSKNELALKDVIIQHSDEIIILLDMFGRILSINP
                     ALTSILKFNKEDVIQHPFRVILFEKSFEDSIRLRDLLLSRFKDVFKGYEAEIVLPCKV
                     QHHSEIKSIAFKLIPVFQDNNLQFILAIGRVIQSDFLTSHYLKTEYAEFIIDNNIRMV
                     NLLSYRLTRNLEPYLEKREIIRIQLGLQEAIINAIEHGNLEIDFETKSRLKKMEGNYW
                     DHVLDICNKDHLEKRKIKVQYHLFIDCVKYIITDEGKGFDWKQYVRAAPDGNLIHTYH
                     GVGLTMLQDIFEVSFNEKGNEITLVKRFS"
     gene            1109..1531
                     /locus_tag="PK348_08265"
     CDS             1109..1531
                     /locus_tag="PK348_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020262093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HXK66244.1"
                     /translation="MAEYRNPVPTVDIIILLEDENGNIKNDEIILIKRKNPPYGWALP
                     GGFVDYGETLEQAAVREAKEETSLDVTLLKQFHTYSDPSRDPRQHTITTVYIAKAKGT
                     PHAQDDAKEIGIFNVNDLPQPIAFDHASIIKDFKNNVY"
     gene            1545..>2280
                     /locus_tag="PK348_08270"
     CDS             1545..>2280
                     /locus_tag="PK348_08270"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HXK66245.1"
                     /translation="MDVSYNLLNLTGKAIDILAKFSKADIRVHDVGRIPKAPVVFVVN
                     HFTRMETFFLPNVIHKITGKYIYSLAHHSFFVGGFGTFMEKVGAVSTKDPLRDVKFIG
                     ALLRGDMDCIIFPEGQMVKDKKLVERGKYMVYNSGIRRPPHTGAAFIALRAQFYREEL
                     KSCKERNACDELNNLMAHFGISEQELSHITNQETYIVPVNITYFPIRARANILNKIAN
                     KLVKALPERIEEELEVEGTMLVEGVDI"
BASE COUNT          782 a          366 c          468 g          664 t
ORIGIN      
        1 ggtttttaat ttctgctacc agggagatag taaccagcaa aaatgaatta gcattaaaag
       61 atgtcattat ccagcattca gatgagatca taatcctgct ggatatgttt ggtaggatat
      121 taagcattaa tcctgcatta acgtcaattt tgaagtttaa taaagaagac gttattcagc
      181 atccattcag agttatatta tttgaaaaat catttgagga ctcaatacgg ttacgtgacc
      241 tgttgttaag ccggttcaaa gatgtcttta aaggatatga agctgaaata gtcttgccat
      301 gtaaggttca acaccattct gaaattaaat caatagcatt taaattaatt ccggtatttc
      361 aggataacaa cttgcagttt attctggcaa ttgggagagt tatacagtca gattttctca
      421 ccagtcatta tctgaaaact gaatatgcag aatttattat cgataacaac atacgaatgg
      481 ttaacctgct atcatatcga cttacacgta acttagagcc atatctggaa aagcgtgaaa
      541 ttatccgtat tcagcttgga ttgcaggaag ccattattaa tgcaatagag catggcaatt
      601 tagagataga ttttgaaaca aaaagcagat tgaaaaaaat ggagggcaac tactgggatc
      661 atgtacttga tatatgcaat aaagatcatc tagaaaaaag aaaaattaaa gtgcaatatc
      721 atctgtttat cgattgcgta aaatatatca ttactgatga gggaaaaggt tttgactgga
      781 aacaatatgt tagagctgct cctgatggca atttaataca tacttatcat ggtgttggat
      841 tgaccatgtt gcaggatatt tttgaagtat cttttaatga aaagggcaat gaaataacac
      901 tggtaaagcg tttttcataa ctaatcttat ttgaagtaat tcaggttgaa ctcacttttt
      961 taaatatgac gatatatttc attacttcaa ggtcactgag tgatacgaca agcggccact
     1021 gagctacgtc gaagtgtggc cactgagctg agtcgaagga ttactctgta tggtttttta
     1081 gcgctaaatt ttttaagagg agatgcccat ggctgaatac cgtaatccgg taccaacagt
     1141 agatataata atattactgg aagatgaaaa tggaaatata aagaatgatg aaattatcct
     1201 gataaaaaga aaaaacccac catatggctg ggcattgcca ggtggctttg ttgattatgg
     1261 tgaaacctta gaacaggcag cagtacgtga agcaaaagaa gaaacttcat tggatgtaac
     1321 attattaaaa caatttcata catattcgga tccatcacgg gatccgcgac agcatacaat
     1381 cacgacagta tatattgcaa aagcaaaagg cacaccacat gcacaggatg atgctaaaga
     1441 aattgggata tttaatgtta atgatctccc gcaaccaatt gcatttgacc atgcatccat
     1501 aattaaagat tttaaaaata atgtatattg aaaaggtaca agccatggat gtatcataca
     1561 atctattaaa cctgacaggc aaggcaattg atatactggc aaagttcagt aaggctgata
     1621 tacgggtaca tgatgttggt agaattccaa aagctcctgt agtatttgtg gtaaatcatt
     1681 tcacccgtat ggaaacattc tttttaccca atgtgattca taaaattaca gggaaatata
     1741 tttattcact tgctcatcat agcttctttg ttggtggatt tggtacattc atggaaaagg
     1801 tgggggcagt gtcaactaaa gaccctctgc gggatgtaaa gtttattgga gcattgcttc
     1861 gtggtgatat ggattgcatc atattccccg aaggtcagat ggttaaggat aaaaaactcg
     1921 tagagcgtgg gaaatatatg gtgtacaatt cggggatacg aaggcccccg cataccggag
     1981 cagcgtttat tgcattacgt gcacaatttt acagggaaga gctgaaaagt tgcaaagagc
     2041 gcaatgcctg tgatgagctt aacaacctga tggcccattt tggaatatct gaacaggaac
     2101 tatcgcacat aacaaaccag gaaacgtata tagtgcctgt taacattacc tatttcccaa
     2161 tcagagctcg tgccaatata cttaataaaa ttgcgaacaa actggttaaa gctctaccgg
     2221 agcgtattga agaagaactt gaagttgaag gaacaatgct tgttgaagga gtagatattg
//
LOCUS       DAOSZP010000381         2275 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_36266_length_2275_cov_2.705856, whole genome shotgun sequence.
ACCESSION   DAOSZP010000381 DAOSZP010000000
VERSION     DAOSZP010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36266_length_2275_cov_2.705856"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="PK348_08275"
     CDS             complement(<1..300)
                     /locus_tag="PK348_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002670738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HXK66246.1"
                     /translation="MTALQMILLLIFVFTTFIGYRLIKNVPSLLHTPLMSGMNALSGV
                     TILGALIATSLSLANGNAIVSWLMGGIAIILAMINVCGGFAVTDRMLKMFSKNMLL"
     gene            complement(329..1489)
                     /locus_tag="PK348_08280"
     CDS             complement(329..1489)
                     /locus_tag="PK348_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004334483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HXK66247.1"
                     /translation="MVIGILKEIMEGEKRVAATPQTVKKMIADGYKVLVETGAGEGSF
                     YNDDEYKAVGATITDTESIYHESEVILKVKEPLFNNILNIHEVDLLHSGQIIISFLHP
                     ASPDNHEMIKKLTAKGVTGLTLDGVPRISRAQSMDALSSMSSCAGYKGMLLAANAIAK
                     FMPMITSAVGVVKPANVLVVGAGVAGLRGIATAKGLGAVVYAADIRPDANEQAKSLGA
                     KIIDTGVPADIAVSQDGKYANKLPEEWLKVERESLKDIIAQADIVFLTALVFGKKAPI
                     LVDEEMVKLMQPGSCIADISIDQGGNCSITQPGVISQKHGVNIIGYKNIPGMLPASST
                     DMFSNNIYNLLKYLTVDGKIKLDLSDEIVKSILVCHEGVLLHEGTLEAMSQK"
     gene            complement(1645..>2275)
                     /locus_tag="PK348_08285"
     CDS             complement(1645..>2275)
                     /locus_tag="PK348_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018281523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding domain-containing protein"
                     /protein_id="HXK66248.1"
                     /translation="YHTAITHLKSPDSFINELYWRDFFTSIAFHFPHVFKGAFHKQYD
                     ALQWANDKEKFELWTNGLTGFPIVDAGMRQLNTTGWMHNRVRMIVASFLVKDLHIDWR
                     WGERYFATKLIDYDPSVNNGNWQWAASTGCDAQPYFRIFNPWLQQKKYDPECEYIKRW
                     IPELQIVSLKDIHNWVYSGSSVKYPKPILDHVIESSYAKGIYKSIFRNE"
BASE COUNT          725 a          459 c          340 g          751 t
ORIGIN      
        1 caataacata ttttttgaaa acattttcag catcctatca gtaactgcaa atccaccaca
       61 tacatttatc attgctaaaa tgatagcaat accacccata agccagctta cgattgcatt
      121 tccattagca agagatagtg atgtagcgat taaagcacct aaaatggtaa cacccgataa
      181 agcattcatt cctgacatca aaggggtatg caataacgaa ggaacgtttt tgataagacg
      241 atatcctata aacgttgtaa aaacaaaaat taataatagt atcatttgta atgctgtcat
      301 atacatttca ccttaataac aataaactct atttttgact cattgcctct aatgttcctt
      361 catgaagcaa tactccctca tggcatacta aaatagattt gacaatttca tctgataaat
      421 ccagttttat tttgccatct actgttagat attttaataa attatatata ttattagaaa
      481 acatatcagt cgaactagct ggcagcatgc caggaatatt cttgtaacca attatattaa
      541 ctccatgttt ttgtgatatc acacctggtt gcgttattga acaattaccc ccctgatcga
      601 tagaaatatc agctatacat gaacctggtt gcattaattt aaccatttct tcgtcaacta
      661 agataggtgc ttttttacca aatactaatg ctgtaagaaa tactatatca gcttgagcaa
      721 ttatatcttt caatgactct ctttctactt ttaaccattc ctcaggtagt ttatttgcat
      781 atttaccatc ttgactgaca gctatatcag caggtactcc agtatcaata attttagctc
      841 caagggactt tgcctgttca tttgcatcgg gtctaatatc tgcagcatac actacagcac
      901 caagcccttt tgctgtagct atacctcgta aacctgcaac acctgcacca accaccaaaa
      961 catttgctgg ttttacaaca cctacagctg aagtaatcat aggcatgaat ttagcaattg
     1021 cattagctgc taaaagcatg cctttataac ccgcgcaaga actcattgat gacaaagcat
     1081 ccatggattg tgccctacta attctgggaa ctccatccaa agtaagacca gttactcctt
     1141 tggcagttaa tttcttgatc atttcatgat tatcaggtga ggccgggtga agaaatgaaa
     1201 taattatttg accggagtgt aacaaatcaa cttcatgaat atttaatata ttattaaata
     1261 aaggctcttt tacttttagt attacttcag attcgtgata aattgattct gtatcagtta
     1321 tagtagcacc tacagcttta tattcatcat cgttatagaa tgagccttct ccagctcctg
     1381 tttcaactag tactttataa ccatcagcta tcattttttt tactgtttga ggagtggctg
     1441 cgacccgttt ttcaccttcc ataatttcct ttagtatacc aattaccata aaatcctcca
     1501 taatgattat ataatatttt caatttaagt atataggtta tcagtattta attcaagtaa
     1561 cagttctcaa taatgagtga gcaatcactc attattgaca tttaaagaaa tacaatagca
     1621 aattgcaaga atttttttat ttttttattc atttctgaat atggacttat agattccctt
     1681 tgcgtaagat gactcgataa catgatctaa tatgggtttt ggatatttca cactactacc
     1741 agaatatacc caattatgaa tatcttttaa agagacgatt tgtaattctg gtatccagcg
     1801 ttttatatat tcacattccg gatcatattt tttttgctga agccagggat taaaaattct
     1861 aaagtatggc tgagcatcac accctgtaga tgcagcccat tgccagttgc cattattaac
     1921 cgacggatca taatcaataa gcttggtggc aaaataacgt tcaccccacc gccagtcaat
     1981 atgcaggtcc ttgaccagaa aacttgccac aatcatccgg accctgttat gcatccatcc
     2041 tgttgtgttg agctgtcgca tacccgcatc aacaattgga aatcctgtga ggccattagt
     2101 ccacagttca aatttttcct tatcatttgc ccactgcagc gcatcatatt gtttatgaaa
     2161 agctccctta aagacatgtg gaaaatgaaa tgcaatagat gtaaaaaaat cgcgccagta
     2221 caattcatta atgaatgaat ccgggctttt taaatgcgtg attgcagtgt gatag
//
LOCUS       DAOSZP010000382         2257 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_36618_length_2257_cov_3.267484, whole genome shotgun sequence.
ACCESSION   DAOSZP010000382 DAOSZP010000000
VERSION     DAOSZP010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2257
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36618_length_2257_cov_3.267484"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..566)
                     /locus_tag="PK348_08290"
     CDS             complement(<1..566)
                     /locus_tag="PK348_08290"
                     /inference="COORDINATES: protein motif:HMM:NF037982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HXK66249.1"
                     /translation="MIPQPPKGFNLLKVLGPGLVWSASAIGVSELVFATRSGALFGYL
                     LLWAPIVSLLMKYAIFELVGRYTIATGENVLIAFAQVEIDCKLFKLKTGWVITLFWIV
                     FIASVSGMAGIALSVGSCIYGLVPAISMEMATVCSIVTVGIILYVGSYITLEKISRAL
                     VFIMIVFLVYALIETAPDPVAMADGLIPR"
     gene            complement(593..1294)
                     /locus_tag="PK348_08295"
     CDS             complement(593..1294)
                     /locus_tag="PK348_08295"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXK66250.1"
                     /translation="MSLAFTLIGQSTVCIDIDGLVFMTDPWWGSFEFLRAVPLPVDPE
                     KIEPIHYMLVSHNHIDHWCDRAIRLAQARDCEVIGSISAVKRAQKKGVKKINALTPGN
                     SVNCNGIMITAVPAFHPFAKDAIGFIIQKGSVTIYFSGDTRYHELLQKALTPYSVTIA
                     MIQVACSTYPIVGKDGMDMEAATRFVDEVKPQIVIPIHYQIKGKYLTKQDLENWSIRA
                     KKIILLNGVKTLIPL"
     gene            complement(1341..>2257)
                     /locus_tag="PK348_08300"
     CDS             complement(1341..>2257)
                     /locus_tag="PK348_08300"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HXK66251.1"
                     /translation="HVVQRLVDENYAVTGIDIKDFPGKGLPNVTFFKGDIVDRDFVVQ
                     SCSGADAVVHCAAALAQFVKDTDRMYAINVTGTRNVIEACQKHTIQQLVFVSSVEVYG
                     IDVPVPCPEDVPLCPVCPYGEHKVIGEQMCIGLAEKGHGVAILRPPTINGPGQNEPFL
                     VEQMKAVREGKPVILPGGGKTRLQMVDVRDVAQAIVLSLHKITKGVLVANISSDNVPT
                     IRQMVTALFEHAGKKEKILSVPAPVARGIVNILSFFGVSPLEPQHLEIALRDYVFDNS
                     RAKKALGWYPTKDDIESAIDAYEWFINN"
BASE COUNT          662 a          537 c          392 g          666 t
ORIGIN      
        1 cgtggtataa gtccatctgc catcgctaca ggatctggtg ccgtttctat caatgcatac
       61 accagaaaca ctatcataat aaaaacaagc gcccgggata ttttttctaa agttatgtag
      121 cttcctacat agagtattat tccaacagtt actatcgaac acaccgttgc catttccatg
      181 gaaattgctg gcaccagtcc atatatgcag cttccaactg ataatgctat tcctgccatc
      241 cccgaaacac ttgcaataaa tactatccag aaaagagtaa taacccaccc cgttttcagt
      301 ttgaatagtt tgcaatctat ctcaacctgt gcaaaagcaa ttaagacatt ttcaccggtt
      361 gcgatggtgt aacgtccaac aagctcaaag attgcatatt tcattaaaag agaaacaatt
      421 ggtgcccaca gcagtaaata tccaaacagt gcaccagaac gagtggcaaa cacaagctct
      481 gacaccccaa tggcacttgc cgaccatacc agaccaggcc caaggacttt caacaggtta
      541 aacccttttg gtggctgtgg tatcatacct cccccctttt tttttagtat atttaaagtg
      601 gtatcaatgt tttaactcca tttagaagta taattttttt tgcccgtata ctccagtttt
      661 ctaaatcctg ttttgttaga tatttcccct taatctgata gtgaatgggt ataacaatct
      721 gcggcttaac ctcatcaaca aaacgagtag cagcttccat atccatgcca tctttgccta
      781 caataggata ggtggagcag gcaacctgta tcatggcgat agttactgag taaggggtca
      841 gagccttttg taaaagctca tggtaccgcg tatcacccga aaaatatatg gtaactgaac
      901 ccttttgtat aataaatcct atggcatcct ttgcaaaagg atgaaatgca ggcactgctg
      961 ttatcatgat accattacaa ttaacactat ttcctggggt cagagcatta atttttttta
     1021 ctcctttctt ttgtgcccgt ttgacagcac tgattgatcc aataacctca caatctcttg
     1081 cctgtgccag cctgattgct ctatcacacc agtgatcaat atgattatgt gaaacaagca
     1141 tataatgaat gggttcaatt ttttcaggat ccacagggag cggaacagcc cgcaagaatt
     1201 caaatgatcc ccaccaggga tcggtcatga agactaatcc atcaatatct atacatactg
     1261 tactttgccc aattaatgta aatgcaaggc tcataggcaa acctcctcag aacatgcgtt
     1321 aatatatttt attatctccc tcaattattt ataaaccatt cgtacgcatc aatcgcactt
     1381 tctatatcat ctttggttgg ataccagcct aatgcttttt ttgctctgct attatcaaac
     1441 acatagtcgc gcaatgctat ctcaaggtgc tgtggttcaa gtggcgatac accaaagaat
     1501 gaaagaatgt taacaatacc ccgtgcaact ggtgcgggaa ccgaaaggat tttttccttt
     1561 tttcctgcat gctcaaataa tgctgttacc atctggcgaa tggttggaac attatcgctt
     1621 gaaatatttg caacaagcac cccttttgtt attttatgta gcgatagcac aatggcctgt
     1681 gcaacatccc gtacatctac catctgcaat cgtgtttttc ctccaccagg gaggataaca
     1741 ggtttacctt ctcttactgc cttcatctgc tcaacaagga agggttcatt ttgtcccggg
     1801 ccattaattg ttggtggcct taaaatggca acaccatgcc ctttctcagc caggccaata
     1861 cacatttgct cccctataac cttatgttca ccatagggac atacaggaca cagtggtaca
     1921 tcctcaggac agggtactgg cacatcaata ccatacacct ctacagaaga tacaaacacc
     1981 agttgttgta tagtgtgttt ttgacatgct tcaatgacat ttctggtacc ggtgacattt
     2041 attgcataca ttctatcagt atctttaaca aattgagcaa gcgctgcagc acaatgtacc
     2101 accgcatccg caccactgca tgattgtacc acaaaatccc tatcaacaat gtcacccttg
     2161 aaaaatgtaa cattgggtaa gcccttgcca ggaaaatctt ttatatcaat gccagtgact
     2221 gcatagtttt catcaacaag cctttgtaca acatgtt
//
LOCUS       DAOSZP010000383         2244 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_36886_length_2244_cov_3.065327, whole genome shotgun sequence.
ACCESSION   DAOSZP010000383 DAOSZP010000000
VERSION     DAOSZP010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2244
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36886_length_2244_cov_3.065327"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>316
                     /locus_tag="PK348_08305"
     CDS             <1..>316
                     /locus_tag="PK348_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002561398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HXK66252.1"
                     /translation="CNEIPALRQLAEKNKVPMGGRDFKTGQTLMKTIIAPGLKARMIG
                     VDGWYSTNILGNRDGEVLDDPESFKTKEVSKLSVLDNIFQPDEYPALYDGYHHVVRIN
                     YYP"
     assembly_gap    317..326
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <327..590
                     /locus_tag="PK348_08310"
     CDS             <327..590
                     /locus_tag="PK348_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004512 - inositol-3-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006021 - inositol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HXK66253.1"
                     /translation="IKVNFLCRDSILAAPVVLDLVLFFDLAQRASMYGIQEWLSFYWK
                     SPMFEPGEEPIHDLFVQLMKLKNTLRWLMDVPPVTYLGNETYD"
     gene            648..>2244
                     /locus_tag="PK348_08315"
     CDS             648..>2244
                     /locus_tag="PK348_08315"
                     /inference="COORDINATES: protein motif:HMM:NF024707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4080 domain-containing protein"
                     /protein_id="HXK66254.1"
                     /translation="MEPTVLLLGINAKFIHSTLSLYYLKQIIASYDYHGTILETTINN
                     PVQETLTSIHAIQPGVCCIAVYIWNAQYIKRLLPLLHVTLPQCKIILGGPEVSYTSND
                     WLKQFPFVDCIIQGPGEKSFEYLANHQFNICEKILHCPNYHFNDIPFPYTSEDLKHLA
                     NHYIYYESSRGCPYSCSYCISSREDQKLQFKDIQKVYDELDVLLHHNVRMVKFVDRTF
                     NCNPTHSRALFTYLLDHNNDHTQFHFEIHPNLLYEEDFEILKWVPPQYFQFEIGIQSI
                     HENTLHAIQRKMNWEKARENIRKLVELQNIHIHLDMICGLPYEDMEGIKESFNAIVSL
                     KPHYFQMGFLKILPGTNIDTKKYEYGIEHEDNPPYMLKKNKWLSHEEMQLLHHIERVV
                     DSLYNHHFNTTSLMLADFIAQDNLFDLYHEIANFFQEHNFSLYAKSWESIAAILLEFI
                     KHSYPEYTKLATDCLRWDWFVKSNNKWVPPFIRSSSNPNTIKEMIIQQNRISKQELSL
                     NKTIVSINDIQRSQIFIAESDHFM"
BASE COUNT          733 a          425 c          386 g          690 t
ORIGIN      
        1 gtgtaatgaa atacctgcat tgcgtcagtt agctgaaaag aacaaagtgc caatgggcgg
       61 cagggatttc aagacaggcc agacattgat gaaaactatt attgcccctg gacttaaagc
      121 cagaatgatt ggtgttgatg gctggtattc aactaacatt ttaggtaacc gtgatggcga
      181 agttcttgat gaccctgaat cattcaaaac taaagaagta agtaaacttt cagttctgga
      241 taatattttc cagcctgatg aataccctgc actgtatgac ggctatcacc atgttgtcag
      301 aattaactac tacccannnn nnnnnnatta aggtcaattt cctgtgccgc gactcaatat
      361 tagctgctcc tgttgttctt gatcttgtac tcttctttga tctggctcag cgtgcatcaa
      421 tgtatggtat tcaggaatgg ctctctttct actggaagag cccaatgttt gaacccggcg
      481 aagagccaat ccatgacctt tttgtccagc tcatgaaatt gaaaaataca ttgcgatggc
      541 tgatggatgt tcctccagta acctacttag gaaatgaaac atatgactga taataataat
      601 aaaaagaggg cttgctaaaa gccctctttt agtatatact tcaatatatg gaaccaactg
      661 tactgttact gggtattaac gctaaattca tccactccac tctatctctt tactatttaa
      721 aacaaattat tgcatcatac gattatcatg gtactatcct tgaaactacc attaataatc
      781 cggtgcagga aacccttacc agtatacacg ccatacaacc tggtgtatgc tgcatagctg
      841 tatatatatg gaatgcacag tatattaaaa ggcttttacc tttgttacat gttacacttc
      901 cacaatgtaa aatcatatta ggtggtcctg aggtaagcta caccagtaat gactggctaa
      961 agcagttccc gtttgttgac tgtatcatac agggacccgg tgaaaaatcc tttgaatatt
     1021 tagctaacca ccaatttaac atatgtgaaa aaattctgca ttgcccaaat tatcatttta
     1081 atgatatccc ttttccgtat acctctgaag atttgaaaca tttagccaat cattatattt
     1141 actatgaatc cagccgcgga tgtccttatt catgcagtta ctgtatatca tcacgtgaag
     1201 atcagaaatt gcagtttaaa gatatccaaa aagtatatga tgaacttgat gttcttttgc
     1261 atcataacgt gcgtatggta aaatttgttg atagaacctt taactgtaat cccacacatt
     1321 cacgtgcgct gtttacatat ttacttgatc ataataatga ccatactcaa tttcattttg
     1381 aaattcatcc taacctttta tatgaggaag attttgaaat attaaaatgg gtgccacctc
     1441 agtatttcca gtttgaaatt ggaattcaat ccatacatga aaatacattg catgccattc
     1501 agcgaaagat gaactgggaa aaagcaaggg aaaatattcg caaacttgta gaattacaaa
     1561 atatccacat ccatcttgat atgatatgcg ggcttcccta cgaagacatg gaagggatta
     1621 aggaaagttt taatgcaata gtaagcttaa agcctcatta cttccagatg ggatttttga
     1681 agatactgcc tggaacaaat attgatacaa aaaaatatga gtacggcatt gaacatgaag
     1741 acaatccacc ttacatgctg aaaaaaaata aatggctttc acacgaggag atgcaacttc
     1801 ttcaccacat tgaacgcgtt gttgatagtt tatataatca tcattttaac acaacatcgc
     1861 taatgcttgc tgacttcatt gcacaagata atctgtttga tttatatcat gaaattgcta
     1921 acttttttca ggaacataac ttttcattgt atgcaaaaag ctgggaatca attgctgcaa
     1981 tactgcttga atttattaaa cacagctacc ctgaatatac taagcttgcc actgattgcc
     2041 tgcggtggga ttggtttgtt aaatcaaata ataaatgggt gccacctttt atacgaagta
     2101 gtagcaaccc gaatactata aaagaaatga ttattcagca aaacagaata tctaagcagg
     2161 aactatcgct taataaaact atagtttcta ttaatgacat acaacgttca cagatcttta
     2221 tagccgaaag tgatcacttt atgc
//
LOCUS       DAOSZP010000384         2232 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_37156_length_2232_cov_3.461644, whole genome shotgun sequence.
ACCESSION   DAOSZP010000384 DAOSZP010000000
VERSION     DAOSZP010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37156_length_2232_cov_3.461644"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..866)
                     /locus_tag="PK348_08320"
     CDS             complement(<1..866)
                     /locus_tag="PK348_08320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66255.1"
                     /translation="MELHDIIKNDDDRLFIISPECFIVFTGDSTEDEKPFIRIGNSLL
                     LTPRVIPLIENIIITDLITGNPACEQYNIDPRYLSSNRYIGSKLIVKRYLEFQKLFGL
                     DLNNATIVDIEQDIPQLSKEQIISDRETFLGVFYTDSNFKILHNQKTMFDLKEIHQKY
                     PGDVLVHSKLSEYSNKSRYAGCGFVITRGATIFYKNNSFSTVGIPSRYYSTFAQLQVD
                     PANIRDVIITNTNSMPLVPLIKWKNAAGGRLRIFYDNDDEIKLLQKLFNQCTLHHKSS
                     KNFVCDNPEGIT"
     gene            complement(853..>2232)
                     /locus_tag="PK348_08325"
     CDS             complement(853..>2232)
                     /locus_tag="PK348_08325"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HXK66256.1"
                     /translation="FSILIPFVAGFIASWYIPAFSGTYKSLPASIFFAIVTSITALPV
                     IAKILLDVKIFHSDLGMIILTSAMVNDITGWILFSSLIKTVETGSIAIISIITSLGLT
                     LLITIIILTIFRYVLNELLPIVQAHTEWPGGVITLGLVLSMFLASLTEAIGIHAFLGA
                     FMGGIILGDSPHLNAHTKEIINQFIDNFFSPLFFVSIGLGINLITQLSLIQAFMITLV
                     VFISNIIASLIAGKISNNTFIDSLAIGVGMSTSGTMGIIVGIFALQYGIINTTTYSSI
                     VVMAIATSLLAGPLLKLILKPKEEVSLIELLDNNFIPECTCTNRNELIQQVSQHIQKR
                     YGYDANKVAHAIIEREQLMSTGIGQYVAIPHARIPECKKPVVICATHPQGLDFDAPDG
                     KPAKVIFMIITPQHDNDVQIFIISKIAQLCSNPNVVLQILNAKNNNEFVAILKTTSHI
                     MEMEGYGTA"
BASE COUNT          699 a          383 c          448 g          702 t
ORIGIN      
        1 atggttatgc cttcgggatt atcgcacaca aaattttttg atgatttatg atgcaacgtg
       61 cactggttaa aaagcttttg taaaagtttg atttcatcgt cattgtcata aaaaatcctg
      121 agtctccctc cagctgcatt tttccatttt atcagtggaa caagtggcat gctgttggtg
      181 tttgtgatta taacatcgcg aatatttgca gggtcaacct ggagttgtgc aaatgtactg
      241 taatagcgcg atggaatacc aacagtacta aaagaattat ttttataaaa gattgtagct
      301 ccacgagtta taacaaaacc gcaaccagca taccgtgatt tgtttgaata ttctgatagt
      361 ttggagtgga ccagtacatc gccaggatat ttctggtgta tttcctttaa gtcaaacatt
      421 gttttctgat tgtggagaat cttaaagtta ctatcggtat aaaaaacccc taagaatgtt
      481 tctctgtcgg agataatctg ttctttggaa agttgtggta tgtcctgctc aatgtcgaca
      541 atggttgcgt tattgaggtc aagaccaaaa agcttttgaa attctaaata gcgcttcaca
      601 ataagcttgc taccaatata ccggtttgac gaaagataac ggggatcaat gttatactgt
      661 tcacaggccg gatttccagt tataagatca gtaataatga tattttcaat gagggggatg
      721 actcgcggtg ttagtagcag gctgttacca atacgtatga atggcttttc atcttctgtg
      781 gaatcacccg taaatacaat aaagcattca ggtgaaatga taaataacct gtcgtcatcg
      841 tttttaatta tgtcatgcag ttccataacc ctccatttcc ataatgtgag atgttgtttt
      901 gagaattgca acaaattcat tattattttt tgcattcagt atttgtaaca caacattagg
      961 atttgaacat agctgtgcta tctttgatat tatgaaaatc tgcacatcat tatcatgttg
     1021 aggtgttatt atcataaaaa ttacttttgc aggtttgcca tcaggggcat caaaatccag
     1081 cccctgtgga tgtgttgcgc atataacgac tggttttttg cactcaggta tgcgggcatg
     1141 aggtatggca acatactgac caatgccggt actcatcagt tgttcccgtt caattattgc
     1201 atgtgcaacc ttgtttgcat cgtatccata ccgcttttgt atgtgctgtg atacctgttg
     1261 tatcagttca ttgcgattgg tgcatgtgca ctcaggaata aaattgttgt ctaaaagttc
     1321 aatcagggaa acctcttctt tgggtttgag tattaatttt aacaatggtc ctgcaagcag
     1381 tgaggttgca atagccataa caacgatgga agaatacgtt gttgtattaa taattccata
     1441 ctgcagtgca aatattccaa caataatacc cattgtaccg cttgtactca ttccaacgcc
     1501 tatagctaaa gaatctatga atgtattgtt gctgattttc ccggcaatca ggcttgctat
     1561 tatatttgaa ataaatacaa ccagtgtaat catgaatgcc tgtataagtg atagctgtgt
     1621 aataagatta atgcccaaac caattgagac aaagaaaagc ggtgaaaaaa agttatcaat
     1681 gaattgattg ataatctctt tggtatgtgc gttgaggtgg ggggaatcac ccaatataat
     1741 gccacccatg aatgcaccca ggaaagcatg aataccaatg gcttcggtta atgatgccag
     1801 aaacattgat aatactaatc ccagagttat gactccacct ggccattcag tgtgtgcctg
     1861 aactatagga agcagttcat taaggacata gcggaaaatt gttaaaatga taattgttat
     1921 caatagtgtt aatcccaacg atgtaataat tgagattatt gcaatagaac cagtttcaac
     1981 tgttttaatt aatgatgaaa ataaaatcca gccggtaata tcgttaacca ttgctgaggt
     2041 taaaataatc attcccaggt ctgaatgaaa aattttgaca tccagtagaa ttttggcaat
     2101 gaccggaagt gcagtaatag atgtgacaat agcaaagaaa attgacgctg gtaacgattt
     2161 ataagtgccg gaaaaagctg ggatatacca cgatgcaata aaacctgcaa caaacgggat
     2221 aagtattgaa aa
//
LOCUS       DAOSZP010000385         2223 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_37340_length_2223_cov_2.594557, whole genome shotgun sequence.
ACCESSION   DAOSZP010000385 DAOSZP010000000
VERSION     DAOSZP010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2223
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37340_length_2223_cov_2.594557"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(167..1006)
                     /locus_tag="PK348_08330"
     CDS             complement(167..1006)
                     /locus_tag="PK348_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018394885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HXK66257.1"
                     /translation="MLIGIVGAGIMGTDIAHLAIENNYEVILHDIDESQLKHAYETIT
                     GHLDRYVEEKRLEQSQRGKIISLLKLHKNIKDLHKADIIIECIPENLECKKNVFKSLD
                     AICKAGTILASNTSSLPITSIASATKRPEYVIGIHFMNPARAMRLVEIVGGIATTRET
                     IESAKSFVKSLGKTPVESKDYPGFVLNRILMPMINEAIFTLYEGAASAETIDKTMKLG
                     LNLPMGPLQLADLIGLDVVLAIIEELYEGFGDPKYRPCPLLKKYVEAGYLGKKTGRGF
                     YSY"
     gene            complement(1018..1983)
                     /locus_tag="PK348_08335"
     CDS             complement(1018..1983)
                     /locus_tag="PK348_08335"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HXK66258.1"
                     /translation="MSKTIVFLDASTVDFGDIDFTPIASLGTFITYPVTKPHETISRS
                     KDADIIITNKVVFYADEIAQLHKCSLIAVAATGYNTIDIKAAKNKNIAVANVPGYSRE
                     AVAQLTLCFILALANNLIKYNAATHDGSWSRSPIFTMGNWPTMCINDKVLGIMGYGDI
                     GKRVAKLAQAFGMKVIALKRENSYIDTSVERYPLEEFCTMADFISIHMPLNESTHHMV
                     NKDFLAMMKPSAYIINMARGPIVDTKALAQALHNNVIAGAALDVLEQEPPDINEPILS
                     APNCIITPHIGWASRETRQALINEIAENIKAFLNGTKRNIVNETV"
     gene            complement(2040..>2223)
                     /locus_tag="PK348_08340"
     CDS             complement(2040..>2223)
                     /locus_tag="PK348_08340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66259.1"
                     /translation="RIIKVIIAKTKELGYKTLYLFEGHNDTVNLIQYYTKRGFSVLEE
                     AIDNDGKPTTIMYINL"
BASE COUNT          627 a          434 c          423 g          739 t
ORIGIN      
        1 gcattagcgc tatcaattat caatgggaaa gcaaaagttg aagtatccgg caatatggat
       61 gaacataaat taaaagaatt ttctgattta cccattgatt atgtgtcaat aggtgcacta
      121 acccattcag ttaaggcatt tgacctttca atgcggttac tgtagcttaa taggaataga
      181 atcctcttcc agtcttttta cccagatacc cagcctcaac atatttttta agaagtggac
      241 atggcctgta ttttggatcg ccaaagcctt catacaattc ttcaatgatt gccagcacta
      301 catcaagccc aatcaaatcc gcaagctgta atggacccat tggcaaattc aggcctaact
      361 tcatggtttt gtcgatagtt tctgcagaag cagctccttc atacagagta aagattgcct
      421 cattgatcat tggcataagg attctgttca acacaaagcc aggataatct tttgattcta
      481 ctggtgtttt acctaatgat tttacaaaac tttttgctga ttcaattgtt tcacgggtag
      541 tagcaatgcc accaactatc tccaccagac gcattgcacg tgcaggattc ataaaatgaa
      601 tgcctatgac atattccggt cgtttagttg ctgaagcaat gcttgtgatg ggcaatgatg
      661 atgtattact tgcaagtatt gtgcctgctt tacatatagc atccagtgac ttgaaaacat
      721 tttttttaca ttcaaggttt tctgggatgc attcaatgat aatatcagct ttatgaagat
      781 ccttaatatt tttatgcaat tttaaaagcg aaattatctt gccccgttgt gattgctcca
      841 atcttttttc ttcaacatac ctgtcaaggt gccctgttat tgtttcatat gcatgtttca
      901 attgtgattc atcaatgtca tggagaatta cctcataatt gttttcaatg gctaaatgtg
      961 caatatcagt acccattatg ccagcaccaa caattcctat taacatacat acctccatta
     1021 tacagtttcg ttgactatat ttctttttgt gccattcaag aacgctttaa tattttcagc
     1081 aatctcgttt ataagtgcct gcctggtttc ccgtgaagcc cagccaatat gtggcgttat
     1141 aatacagttg ggtgctgaaa gaataggttc attaatatca ggtggttcct gttcaagcac
     1201 atccagagct gcaccggcaa tcacgttatt atgaagtgcc tgtgcgagcg cctttgtatc
     1261 aacaataggg cctcgtgcca tgtttattat atatgctgat ggtttcatca tagcaagaaa
     1321 atctttgttg accatatggt gtgtggattc attgagcggc atgtggatag atataaaatc
     1381 agccattgta caaaattctt caaggggata tcgttcaact gaggtatcaa tgtaggagtt
     1441 ttcccttttt agtgcaataa ccttcatgcc aaatgcttgt gccagttttg ccactcgttt
     1501 cccaatgtca ccgtaaccca tgatgccaag cactttatca ttgatgcaca tggtaggcca
     1561 gttgcccatg gtaaaaatgg gtgagcggct ccagcttcca tcatgggtgg cagcattgta
     1621 tttaataaga ttattagcaa gggctaaaat aaaacacaga gtgagctgtg caaccgcttc
     1681 gcgtgagtag ccgggaacat tggcaacggc aatgttttta ttctttgctg ctttgatatc
     1741 tatagtgttg tagccagttg cagcaacagc tatgagtgag cacttatgta attgagctat
     1801 ctcatcagca taaaagacaa ccttgttggt aataataata tcagcatcct tgcttcgcga
     1861 tatggtttcg tgtggctttg tgacaggata ggtaatgaat gttcccagag atgcaatggg
     1921 agtgaaatct atatccccaa aatcaaccgt tgaagcatct aaaaatacaa ttgttttact
     1981 catacggtat ttcctttcaa ctgtatgtat tgcattatca tggacctgtc aatagtttat
     2041 tacaggttaa tatacataat tgtggttggt ttgccatcat tgtcaatggc ttcttcaagc
     2101 acagaaaatc ctcgttttgt ataatactgt ataagattga cagtatcatt gtgtccttca
     2161 aaaaggtaaa gtgttttgta ccccaattct tttgttttag caattattac tttgattatc
     2221 ctg
//
LOCUS       DAOSZP010000386         2222 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_37358_length_2222_cov_3.596216, whole genome shotgun sequence.
ACCESSION   DAOSZP010000386 DAOSZP010000000
VERSION     DAOSZP010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2222
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37358_length_2222_cov_3.596216"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="PK348_08345"
     CDS             <1..267
                     /locus_tag="PK348_08345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66260.1"
                     /translation="DPEIFPMFTNKDPYERINNNSVTMIKNILKEGIGKGLFRPVDIS
                     AMSKAIFSIYKMFVIRMYIRSEEKDVRSMFEQTLELITQGLFKQ"
     gene            304..705
                     /locus_tag="PK348_08350"
     CDS             304..705
                     /locus_tag="PK348_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HXK66261.1"
                     /translation="MPQELMYLTKEWRDEAERRLKNELTPEKMNFITSSMSNIYLNCP
                     DGKNKYLFFKFIDGKLDTLALGEGEPPDAEFRISGDYQVFAMISRAELGSQKALMTGK
                     LKLKGNMVKALKLAAIADRLNKVLSQIPAQY"
     gene            744..1970
                     /locus_tag="PK348_08355"
     CDS             744..1970
                     /locus_tag="PK348_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HXK66262.1"
                     /translation="MTSRLENKGPYYIDFPGRIKAIQQEMAKEHIDVYLGSRLRTLSW
                     TLDAFCPWRSFVVIPNEGMPAAFTFVIDAARVADESWLDEDHVFGFVPMGGQDQIELI
                     AEYIKDLLKNKKGRIGVENGMSNYLPEGNLTHYEYERFKDALPDCEFVDAYTIIDKLS
                     MIKDEGTINRFREASRIVDVGHKAVYEAISNGGWKGMTETEIAGLAAYAMRKEGSEWE
                     WSYTGGNEIASGYRTGLAGGACTPATRKKLQAGEPLMVDIHAMFMLGLGDHSHNYLIE
                     PVTDRQRWHAQNFLDIVNLTLKTYRAGITPSLLAEQMMAFAEERGFADYMVPGFEHGI
                     GMMGDEWRIGLNDGPFPFWTNPDHVYQENELIICAMQYACPQDNIGFRYENPIVIHKD
                     GCEPLSKYPLTIDKIQ"
     gene            1974..>2222
                     /locus_tag="PK348_08360"
     CDS             1974..>2222
                     /locus_tag="PK348_08360"
                     /inference="COORDINATES: protein motif:HMM:NF014009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HXK66263.1"
                     /translation="MAVITGYISIATQGQGDIIDITLDAQKIVTRNKIQDGLLCLFVP
                     GSTAAITTIEFEPGLQKDINLFLEKIIPRNANYHHHDTW"
BASE COUNT          724 a          388 c          482 g          628 t
ORIGIN      
        1 gaccctgaga tattccccat gtttactaat aaagacccct atgaacgcat taataataat
       61 tcagttacca tgataaaaaa tatcttaaaa gaaggaattg ggaaaggcct ctttaggcct
      121 gtggatatat cagcaatgtc aaaggcaatt ttttctattt ataagatgtt tgttatacgc
      181 atgtacatac ggagtgaaga aaaagatgtg cgttcaatgt ttgagcaaac actggaactt
      241 attacacagg gtttgtttaa acaataactt aatataataa tttttatttt taggaggtaa
      301 cttatgcccc aggaattaat gtatttaaca aaagaatggc gagatgaagc cgaacgacga
      361 ctgaaaaatg aactaacacc tgaaaagatg aattttatca catcatccat gtccaatatt
      421 tatttaaatt gcccggatgg taagaacaaa tatctttttt tcaaatttat tgatggaaag
      481 cttgatacac tggctcttgg tgagggtgaa ccacctgatg ctgagttccg tatatccggt
      541 gattaccagg tttttgccat gatttccaga gctgagttag gttcacaaaa agccctcatg
      601 acaggcaaat taaaattaaa agggaatatg gtaaaagcat taaaattagc agccatagca
      661 gacaggttaa ataaagtatt atcgcaaata cctgcacaat attaatgtac catcctaaat
      721 tttaacatta tgaggtatat actatgacat cacgattaga aaataaaggg ccatattata
      781 ttgactttcc tggcagaatt aaggctatcc agcaggaaat ggcaaaggaa cacattgatg
      841 tttatttagg ttcacggtta cgaacattat cctggacttt agatgcgttt tgcccatgga
      901 ggagttttgt tgttattcct aacgaaggga tgccagctgc gtttaccttt gttattgatg
      961 ccgcacgtgt tgctgatgaa tcatggcttg atgaagacca tgtctttgga tttgtaccaa
     1021 tgggtggaca ggatcagatc gaacttattg ctgagtatat caaagacctg ttaaaaaata
     1081 agaagggaag aatcggtgta gaaaacggga tgtccaatta tctgccagaa ggcaacctaa
     1141 cacactatga atatgaacgg tttaaagatg cactgcctga ttgtgaattt gtagatgcat
     1201 acactataat tgacaaactg tccatgataa aagatgaggg gaccattaac agattccgcg
     1261 aagcatcccg tattgttgat gtgggtcata aagccgtata tgaagccata agcaatggcg
     1321 gctggaaagg tatgaccgaa actgagattg ctggacttgc agcgtatgcc atgcgcaaag
     1381 aaggtagtga atgggaatgg agttataccg gtggaaatga aatagcatct ggatacagaa
     1441 caggacttgc tggtggagcc tgcacacctg caacccgcaa aaaattgcaa gcgggcgaac
     1501 ccctgatggt tgacattcat gcaatgttca tgttaggtct gggggatcat tcacataact
     1561 atctgataga acctgtaact gaccggcaac gctggcatgc acaaaatttt cttgatattg
     1621 taaacttgac acttaaaaca tatcgtgctg gaataacacc ttcattgtta gccgagcaga
     1681 tgatggcatt tgctgaagag cgtggctttg ctgattacat ggtgccaggt tttgaacatg
     1741 ggatagggat gatgggtgat gaatggcgta ttggattaaa tgatggacct ttcccattct
     1801 ggaccaatcc tgaccatgta tatcaggaaa atgaattaat tatctgtgca atgcagtatg
     1861 catgcccaca agataacatt ggattcaggt atgaaaatcc tattgtcatt cataaagatg
     1921 ggtgtgaacc gttatcaaaa tacccactca ctatagacaa aattcaatag attatggcag
     1981 ttattaccgg ttacatatcc attgcaacac agggccaggg tgatattatt gatatcaccc
     2041 tggatgccca gaagatagtt actaggaata aaattcagga tgggttgctg tgcctttttg
     2101 tgcctggatc aacggcagct atcaccacaa ttgagtttga gccgggacta cagaaagata
     2161 taaatctttt tttagaaaaa ataatcccgc gtaatgccaa ctatcatcat catgacacct
     2221 gg
//
LOCUS       DAOSZP010000387         2218 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_37441_length_2218_cov_3.471567, whole genome shotgun sequence.
ACCESSION   DAOSZP010000387 DAOSZP010000000
VERSION     DAOSZP010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37441_length_2218_cov_3.471567"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..681
                     /locus_tag="PK348_08365"
     CDS             <1..681
                     /locus_tag="PK348_08365"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164458.1"
                     /note="catalyzes the reaction of glycine with
                     5,10-methylenetetrahydrofolate to form L-serine and
                     tetrahydrofolate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HXK66264.1"
                     /translation="LLTDIAHIAGLVATGHHPSPVPVADFVTMTTHKTLRGPRGGLIL
                     AKQKYEGMMNKYIFPGIQGGPLMNTIAAKAVAFGEALQPSFKEYSAKVIANAKVLADT
                     LLSRGLTLVSGGTDNHLMLVDLRNKNLTGRDVANRLDEAGITCNKNGVPFDDKSPMVT
                     SGIRLGSPALTTRGLGEDEFRLIGTLICDIIDNMDDEKVLNRVRSQVQELCKAYPTDF
                     LRLEPRGI"
     gene            882..1871
                     /locus_tag="PK348_08370"
     CDS             882..1871
                     /locus_tag="PK348_08370"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HXK66265.1"
                     /translation="MKKLVAVILMCSIAVVGCKKEKKFTVAREGLVNFVAGTVLLVSG
                     GAESKAQIGDAIKEGMTIKTVGPKSMAEIYFGENAVKILGDTVIKVEKLITNLDSNTE
                     ESSFIVEKGRMFSKVTKKLAKGDSYQVKTPTAIAAVRGTDFLVTQEEDKSNVACLDGL
                     LEVLNQSLATGQPIVLEEKQEVDVVPGQDMVAKQISEDKLRMLNILLEIKAMREDIRK
                     KYEQQREEILKYVEEQKKADKTMLEEQREKDKALVEEQKARDKAMIEGIKGEIKDESG
                     KAAQQSLDAAKSQMQDAKAVDKDQASTEAKDQMSAMKPKIEKPKVDLNQFKTQ"
     gene            complement(1962..>2218)
                     /locus_tag="PK348_08375"
     CDS             complement(1962..>2218)
                     /locus_tag="PK348_08375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66266.1"
                     /translation="GVPYLLFINPSTACCGIASLQVLARNPTCRTSLYGTSLPITPFL
                     YNLAGFNNNYYYFILTIISLNFLLQEITLYSFYPKILFSL"
BASE COUNT          726 a          374 c          560 g          558 t
ORIGIN      
        1 ctgttaacag atattgcaca tattgcagga cttgttgcaa ctggacacca tccttcgccg
       61 gttccggtag ccgattttgt caccatgaca acacacaaaa cgctacgtgg gccacgggga
      121 ggtcttattt tagcaaaaca aaaatatgaa ggtatgatga acaagtatat cttccccggc
      181 attcagggtg ggccgcttat gaataccatt gcagccaagg cagtggcatt tggtgaggca
      241 ttgcagccat cattcaagga gtattcggca aaagttattg ccaatgcaaa agtgctggcc
      301 gatacattat tgtcgcgtgg gcttaccctt gtaagtggcg gcaccgataa tcacctgatg
      361 ctggttgacc tgcgaaataa aaatttaacg ggcagagacg ttgctaacag gctggatgaa
      421 gctggtataa cctgtaacaa gaacggcgtt ccctttgacg ataaatcgcc catggtaacc
      481 agcggcatac gtttaggaag ccctgctctg acaacccggg gtcttggtga agatgaattc
      541 aggcttatag gcactttaat ctgtgatatt attgacaaca tggatgatga aaaggttcta
      601 aaccgtgtgc gctcacaggt gcaggagtta tgcaaagcct atccaactga ttttttgcgt
      661 ctggagccca ggggaatata gtgcttgact gtgcaggtca tgtgtagtat gtttcatgta
      721 ttgtaagatt tcaagagagg ttcagaatac taaaaaaggg ttttatcctt aaatgtaatt
      781 ctggatggct ttatatcaat agattccaga tgaggtttgg gaaaattttt gatttcacta
      841 tattcactga taaataagta atactatttt tggaggagta tatgaaaaaa ctagtagcag
      901 ttatcttgat gtgttccatt gcagtggttg gttgtaaaaa agaaaagaaa tttactgtag
      961 cccgagaagg actggttaac tttgttgccg gtacggtact tcttgtttca ggtggtgctg
     1021 aaagcaaggc ccagattggc gatgcaataa aagaaggcat gaccattaaa accgttgggc
     1081 caaaatccat ggctgaaatt tattttggtg aaaatgcggt aaagatttta ggtgatactg
     1141 ttatcaaagt tgaaaagctc attaccaatc tagactctaa cactgaagag tcttcgttta
     1201 tcgttgaaaa aggacgtatg ttctcaaagg taacaaagaa gttggccaag ggcgatagtt
     1261 accaggtaaa aaccccaacg gcaatagcag ctgttcgtgg taccgatttt ctggtaacac
     1321 aggaagaaga caagagcaat gttgcctgtt tagacggctt gcttgaagtg ttgaaccaga
     1381 gtttagctac gggccagcct atagtactgg aagaaaagca ggaagttgat gttgttccgg
     1441 gacaggatat ggttgcaaag cagatatcag aagataagct gcgcatgctc aatatcttgc
     1501 ttgaaattaa ggcaatgcgc gaggatatac gcaagaaata tgagcagcaa cgtgaagaga
     1561 tactgaaata tgttgaagaa cagaaaaaag ctgacaaaac aatgctagaa gagcagcgcg
     1621 aaaaggataa agcactcgtt gaagagcaga aagcccgtga caaggcaatg atagaaggca
     1681 ttaagggtga gataaaggat gaatcgggca aagcagctca gcagtcactt gatgcggcaa
     1741 agagccagat gcaggatgca aaagctgttg ataaggatca ggcaagcact gaagccaagg
     1801 atcagatgag tgcaatgaag ccaaaaattg aaaagccaaa agtagatttg aatcagttca
     1861 agacacagta ataactgtaa tataagatag ataatggggc tattatcaca cgtggtaata
     1921 gccctacctg tttaccggta cgtgaatata tgtttaaagt ttcacaggga aaagagaatt
     1981 ttagggtaaa aagaatagag agtgatttcc tgtagtaaaa agtttaaaga aataattgtt
     2041 aagataaagt aataataatt attgttaaac ccagcaagat tgtataaaaa tggagtaatg
     2101 ggcaaagacg tgccgtacag ggaagtacgg cacgttgggt tgcgtgcgag gacctgaagg
     2161 gatgctattc cgcagcacgc agtggaagga ttgatgaata gcaagtaagg cacaccgc
//
LOCUS       DAOSZP010000388         2218 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_37444_length_2218_cov_3.167822, whole genome shotgun sequence.
ACCESSION   DAOSZP010000388 DAOSZP010000000
VERSION     DAOSZP010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37444_length_2218_cov_3.167822"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..424
                     /locus_tag="PK348_08380"
     CDS             <1..424
                     /locus_tag="PK348_08380"
                     /inference="COORDINATES: protein motif:HMM:NF020260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66267.1"
                     /translation="AVQQLIMPVVMELSPDLLIHIHSYKDILTNYGIIIDQFDEHSIV
                     INAIPDFCNTNDIEGLMRDIVDSLHENKYSLNNIKIKIIEKIACHSAKRANDIITQDD
                     IIAIADYVLSTNDFRCPHGRPFVYIVSKSELERFFKRS"
     gene            421..957
                     /locus_tag="PK348_08385"
     CDS             421..957
                     /locus_tag="PK348_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016753055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HXK66268.1"
                     /translation="MKQKIILGISSSIAAYKACDLARLLTKDGYDVFCVLTENATHLV
                     APLTLQTLTGNPVYTTSFAIQWREMGHIELKENAALLLVAPATANIIGKFANGIADDL
                     LSRTFLAVNCPVVIAPAMNPNMWKHKAVQENIKKLKEWGCIFVEPETGLVACGDEGQG
                     RLASIEEIYKVAINAITR"
     gene            941..1609
                     /locus_tag="PK348_08390"
     CDS             941..1609
                     /locus_tag="PK348_08390"
                     /inference="COORDINATES: protein motif:HMM:NF016051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HXK66269.1"
                     /translation="MQLQDKKIIVTGGPTREWIDPVRYISNASSGKMGIAIADAAYKQ
                     CKELVFIHGPIDESLISGKPYRCKGVESTVDMLEAIKQELSPNMVLIMAAAPADYKPV
                     TKSPTKIKKTNENLTIELQRNPDILKTIASIKKQQQEFNNIVLIGFAAETTDVIAYGK
                     TKLVEKELAMICINDVSRSDTGFGSDFNEVTVMLANGMIINLHRMTKREVAEGIINII
                     INEL"
     gene            complement(1604..>2218)
                     /locus_tag="PK348_08395"
     CDS             complement(1604..>2218)
                     /locus_tag="PK348_08395"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXK66270.1"
                     /translation="VLDNQKVAIIGHNGSGKSTLLLLLSALLLPTSGSITILDMQVTA
                     RNKSQVRKSIGIVFSQVEYQFIMPDLLNDVILSIREGSKEEKQLHALQWLKKVNMDDM
                     LYHSPLALSSGQMKRAALVSVLAKNPKILLLDEPLANLDKPSADAVIHILSDLNMPMI
                     FATHSAYAVQHLANRLIVLEHGTIAYDGVCNSKILEKHKKTILL"
BASE COUNT          760 a          382 c          408 g          668 t
ORIGIN      
        1 agcagtacag caacttatta tgccagttgt tatggaattg tcgcctgatt tgcttattca
       61 tatacattcc tacaaagata ttctcactaa ctacggaatt atcatagacc aatttgatga
      121 acattcaatt gttatcaatg ctatacctga tttttgcaat accaatgata tagaaggatt
      181 aatgagagat attgtcgata gtttgcatga aaacaaatat tctttaaata atattaaaat
      241 taaaataatt gaaaaaattg cttgccattc agcaaaacgt gctaatgata taataacaca
      301 ggatgacatt attgcaatag ccgattatgt actttcaaca aatgacttcc ggtgccctca
      361 tgggcgacca tttgtgtaca tagtatcaaa atctgaatta gagaggtttt tcaagcgatc
      421 atgaaacaaa aaataatttt aggaatttct tcatcaattg ctgcatataa agcctgtgat
      481 ctggcacgtt tgcttactaa agatggatat gatgtattct gcgtacttac agagaatgcc
      541 acacatctgg tagccccatt aaccctgcaa acactcaccg gaaacccggt atacaccaca
      601 agttttgcca tacaatggcg cgaaatgggc catattgaat taaaagaaaa cgctgcgtta
      661 ttactggttg cacctgcaac agcaaacatc attggtaaat ttgccaatgg tattgctgat
      721 gacctgctca gtagaacatt tttagctgtt aattgtccag tggtgatagc tcctgccatg
      781 aatcctaata tgtggaaaca caaagctgta caggaaaata ttaaaaaatt aaaggaatgg
      841 ggctgtatat ttgttgaacc tgaaacagga cttgttgctt gcggtgatga agggcagggc
      901 aggttggctt caatagaaga aatttataag gtagcaatta atgcaattac aagataaaaa
      961 gattatagtt actggcggtc caactcgtga atggattgac cctgtacggt acatatccaa
     1021 tgcttccagt ggcaaaatgg gaatagctat agctgatgct gcatataaac aatgcaaaga
     1081 attagttttt attcatggac ctattgatga atcactcatc agcggaaagc catatcgatg
     1141 taaaggtgtt gaaagtactg ttgatatgct tgaagcaatt aaacaggaac tatcgcccaa
     1201 tatggtattg ataatggctg cagcacctgc agattacaaa cctgtaacta aatcacccac
     1261 aaagataaag aaaactaatg aaaacctgac aatcgaacta caacgcaatc cagatatttt
     1321 aaaaacaata gccagcataa aaaagcaaca acaagagttt aataatattg ttctgattgg
     1381 gtttgcagca gaaacaactg atgtgattgc gtatggtaaa acaaagcttg ttgaaaaaga
     1441 actggcaatg atctgcatca atgacgtaag cagaagtgat acaggttttg gtagcgattt
     1501 taatgaagtt acggtaatgc ttgcaaatgg catgatcatc aaccttcata gaatgacaaa
     1561 aagagaagtg gctgaaggca taatcaatat tattattaat gaattatagt aatattgttt
     1621 ttttatgttt ttctaaaatc ttgctgttac atacaccatc ataagcgata gtaccatgtt
     1681 ccagaacaat aagtctgtta gctaaatgtt gtactgcata ggctgaatgt gtggcaaaaa
     1741 tcattggcat gttaagatct gataaaatat gtatcaccgc atctgccgat ggcttatcta
     1801 aatttgccag gggttcatca agcaatagta tttttggatt ttttgctaaa acacttacca
     1861 gagctgctcg tttcatctgc ccacttgaaa gtgctaaagg actatgataa agcatatcat
     1921 ccatatttac tttttttagc cactgtagtg catgtaattg tttttcttct tttgagcctt
     1981 cacgtatact caatatcaca tcatttaaca ggtcaggcat gataaattgg tattcaacct
     2041 gagaaaatac tatgccgatg gattttctaa cctgtgattt gtttctagca gtaacctgca
     2101 tatcaagaat tgtaatagaa ccacttgttg gtaatagcaa tgcagataac aacaatagta
     2161 atgttgattt acctgaacca ttatgcccaa taatagcaac cttttgatta tccaatac
//
LOCUS       DAOSZP010000389         2217 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_37473_length_2217_cov_2.855689, whole genome shotgun sequence.
ACCESSION   DAOSZP010000389 DAOSZP010000000
VERSION     DAOSZP010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2217
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37473_length_2217_cov_2.855689"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..359)
                     /locus_tag="PK348_08400"
     CDS             complement(<1..359)
                     /locus_tag="PK348_08400"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HXK66271.1"
                     /translation="MENNGTEKIRKGEILILQGEEPRFIYYLQSGSVEILSAPSEYEG
                     LDSKIIVEKSKRVGIINEKSVIAGLSLLFTEPYKKTIRAIEDCVVKKYPLREGGFRQI
                     VVDDPSFAALLLTHVLKR"
     gene            complement(516..863)
                     /locus_tag="PK348_08405"
     CDS             complement(516..863)
                     /locus_tag="PK348_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HXK66272.1"
                     /translation="MGDRSKKFKPKELHFVDSGFSDLETHVKREKQKARELRHSSWWK
                     KRVAPGICHYCGRKFKPSELTMDHVIPLSRGGYSEKINIVPSCKECNIKKKSLLPVEW
                     EEYIQNLKKIGMK"
     gene            complement(869..1801)
                     /gene="fmt"
                     /locus_tag="PK348_08410"
     CDS             complement(869..1801)
                     /gene="fmt"
                     /locus_tag="PK348_08410"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HXK66273.1"
                     /translation="MKIGFFGTPEIASYCLEHLIKHYEIAFVVTSPDKPKGRGKHLLP
                     PPVKEKALEYGIVCFQPTTLKDEQLTETLSQFNCDIFVVVAYGKLIPRSIFSIPPLQT
                     INLHPSLLPKYRGAAPVEWALYNGEQQTGVTVQRINEELDAGDIVLQSTVTIAPNETA
                     QDVYEKILPLGADMLVRAIAGLHDGTITPVPQDHSQATYCGKITSETARINWDIPSIH
                     IHNMVRAFNPKPVAWTTFRGKIIKIWKTLLSDSSCNNSTARPGTVVVQAKRLFVCTFD
                     GTIEVMSLQPETKKPMDASAFINGYRLQHGDAFE"
     gene            complement(1801..>2217)
                     /gene="def"
                     /locus_tag="PK348_08415"
     CDS             complement(1801..>2217)
                     /gene="def"
                     /locus_tag="PK348_08415"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF001159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HXK66274.1"
                     /translation="TDEMFEIMHKEQGIGLAAPQIDVPLQLLVIDIRSNKGPMMALIN
                     PKITAFSDETDGYEEGCLSLPGITADIIRPVSIQVEGIAINEKPVKFEASGLLARVLQ
                     HEIDHLHGKLFIDYLEDYKRKELNAQLKKIKKLNRA"
BASE COUNT          625 a          464 c          388 g          740 t
ORIGIN      
        1 cgttttaaca catgagtaag caataaagca gcaaaagaag ggtcatcaac aactatctgg
       61 cggaatcctc cctcacgtaa gggatacttt ttgactacac aatcctctat ggcacgtata
      121 gtctttttat atggttcagt aaataaaagg cttaagccag ctataacact tttttcattt
      181 attattccaa ctcgtttgct tttctcaaca ataatcttgc tatcaagccc ttcatattct
      241 gaaggtgccg ataatatttc aactgatccg ctctgtaaat aataaataaa ccgtggttct
      301 tcaccctgta aaattaaaat ctcgcccttt ctgatttttt cagtgccatt attttccata
      361 atcttttttt aacacaaaag gtatttttta ctatttaatt aatttttgca ttagtgtaga
      421 gtactattgc aaaaagagca ataacaattt tacttaaaac caattttttt tgggtatgtg
      481 ctcgcaacat acaaagaata gttataataa caattttact tcataccaat tttttttaaa
      541 ttctgtatat attcttccca ttcaacaggc aacaatgact ttttctttat attgcattcc
      601 ttacaactgg gaacaatatt tattttttct gaatatccac ctcgtgaaag tggtattacg
      661 tgatccatcg tcagttctga tggtttaaat ttcctgccac aatagtggca tataccaggt
      721 gccacccttt ttttccacca cgatgagtgc ctgagttccc gggctttttg cttttcacgc
      781 tttacatgtg tttccagatc cgaaaatcca gaatccacaa aatgaagttc ttttggttta
      841 aatttttttg atctgtcacc cataccgttt actcaaaagc atcaccgtgc tgcaatcggt
      901 agccattaat aaaagcgctt gcatccattg gctttttggt ctcgggttgt aatgacatca
      961 cttcaatagt accatcaaaa gtgcacacaa atagacgctt tgcctgcacc accaccgtac
     1021 ccgggcgggc agtggaatta ttacaggagc tatcgctaag gagggttttc catattttta
     1081 taattttacc cctgaatgtt gtccatgcta ctggttttgg attaaatgcc cgcaccatgt
     1141 tatgaatgtg tatcgatggt atatcccagt ttattcgcgc agtttcactg gtaatttttc
     1201 cacaataggt tgcctgcgaa tgatcctgtg gcactggtgt aatagtgcca tcatgcaaac
     1261 ctgcaatagc tcttaccagc atatcagcac caaggggcag tattttttca tatacatcct
     1321 gtgcggtttc attgggggcg atagttaccg ttgattgaag aacaatatca cctgcatcaa
     1381 gctcttcatt aatacgttgc acggtaaccc cggtctgctg ttcaccgtta tacagcgccc
     1441 attcaaccgg tgcagcacca cgatactttg gcaaaagtga tggatgcaaa ttaatagttt
     1501 gcagtggtgg gattgaaaag attgaacgag gaataagttt gccataggct acaacgacaa
     1561 atatgtcgca attgaattgg ctcaacgttt ctgtaagctg ttcatccttt aaggttgttg
     1621 gctgaaaaca cacaattcca tattcaaggg ctttttcttt tacaggagga ggcaacagat
     1681 gtttgcctcg accttttggt ttatcaggag atgtaacaac aaaagctatc tcataatgtt
     1741 ttataagatg ttctaaacag taagaagcaa tttcaggagt tccaaaaaag cctattttca
     1801 tcatgccctg ttaagttttt ttatcttttt caactgtgca ttgagctctt ttcgtttgta
     1861 atcctctaag taatctataa acaatttgcc atgtaaatgg tctatttcat gctgtaatac
     1921 ccgcgccaag agtcctgatg cttcaaattt tacaggtttt tcattgatgg ctatgccttc
     1981 aacctgaatt gaaacagggc gtataatatc agctgtaata ccaggaagtg acaggcatcc
     2041 ttcctcataa ccatccgttt catcagaaaa tgctgtgatc tttggattta taagtgccat
     2101 catagggcct ttatttgacc gtatatctat aactaaaagc tgtagcggaa catcaatttg
     2161 cggagctgcc aggcctatac cctgctcctt atgcatgatt tcaaacatct catcggt
//
LOCUS       DAOSZP010000390         2217 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_37481_length_2217_cov_2.321462, whole genome shotgun sequence.
ACCESSION   DAOSZP010000390 DAOSZP010000000
VERSION     DAOSZP010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2217
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37481_length_2217_cov_2.321462"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1254
                     /locus_tag="PK348_08420"
     CDS             <1..1254
                     /locus_tag="PK348_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011997935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit R"
                     /protein_id="HXK66275.1"
                     /translation="KFFNRGLVVPRPPAGNSFAAEITLILEADPKISVQVDVSGRVQV
                     MESSTKGLQMVDVQAGRPQAIPSESLDLVDWQKVYIEVLAYKEQKGFCNLIIKPETLR
                     KIIEFKNGQGVPICTVIADEQVIRPLLFSDRALLQDAVIHLLKSYIDRFYQVNWEQWD
                     EKRLEYKKLDISDTNFGFRPPGIAEDKIAYMIKVPRSEYQLIDKLQQLLKEQELLYSQ
                     ENSGLPRLNFDRHLYQPLLVDVLQRAKIVPPGLKESEAQFVCDLRDYWKVEKDRALVN
                     SEIFLLRNLSRGYGIGFFEERGFYPDFILWIIDQITKAQRIIFIEPHGMIYAKSYTYD
                     EKARLHERLSQLAKKIGKQKVNIDIFLDAFIISATPYDELYKRYDDGSWDRAKFAEKH
                     ILFQERSLDYDYMKLLFEKSMCVLT"
     gene            complement(1334..>2217)
                     /locus_tag="PK348_08425"
     CDS             complement(1334..>2217)
                     /locus_tag="PK348_08425"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HXK66276.1"
                     /translation="YLLTGRIIDKDKCTLCRKCVDICPKEVLSIKDNEIVIVHDDCIL
                     CSHCYCVCKFNAIHFNELKQPVFSFGHTAVIKDEIKPFDIISFIHSRRSVRRFKADPV
                     NTEMLHDLIEAAVLAPSASNCQRWQFVVINGREKVLMLAEDIKNFFIKLNTIAGNPIL
                     RYLSVIFAGKKILQYYKNNYESVKLGLERAKEGKDLLFHGAPCVIIVHSDMEGSMPVE
                     DGQYAAYNIALVAHSMGLGTCFIGYASATLNSAPSLKEKLHISEHNRVHAVLALGYPQ
                     VEFVKNALRKDYDVKIV"
BASE COUNT          713 a          399 c          427 g          678 t
ORIGIN      
        1 aaatttttca atcgtgggtt ggtggtgcca cgaccaccag ctggcaatag ttttgctgca
       61 gaaataacat taatactgga agcagatcca aaaatatctg tacaggtcga tgtatctgga
      121 cgtgtacaag taatggaaag ctctacaaaa gggcttcaaa tggtggatgt acaagctgga
      181 cggccacagg ctattccttc cgaaagtctt gatctcgttg attggcaaaa ggtctatatt
      241 gaagtacttg cgtataagga gcaaaaagga ttttgtaacc ttataatcaa acctgaaaca
      301 cttcgtaaga ttattgaatt caaaaatggg caaggtgttc caatatgtac ggtcattgct
      361 gatgagcaag taatccgtcc gctattgttt agcgatcgtg ctttactcca ggatgcagtg
      421 atacatttgc ttaaaagcta tattgataga ttttaccagg ttaactggga gcaatgggac
      481 gagaagagac tggaatacaa gaagttagat ataagcgata ccaactttgg ttttagaccc
      541 cctggcattg cggaagataa aattgcctat atgataaaag taccacgatc agaataccag
      601 ttgatcgaca aattgcaaca attgttaaaa gaacaggagt tgttgtatag tcaggaaaat
      661 tctggattgc cacgtttaaa ctttgatcgt catctatatc aacctctatt agtggatgtg
      721 cttcaaagag ctaagatagt tccaccaggc ttaaaagaaa gtgaagcgca atttgtatgt
      781 gatttgcgtg actattggaa ggttgaaaaa gatagagcat tggtaaacag tgaaatattt
      841 ttgctgcgaa atttgagccg tggttatgga attggatttt ttgaggaacg aggattttac
      901 cctgatttta ttctgtggat catagatcaa attacaaaag ctcaaaggat aattttcatt
      961 gaaccacacg gtatgatata tgcaaagtca tacacttacg acgaaaaagc tcgcttacac
     1021 gagcggttgt cacaattggc aaaaaaaatt ggaaaacaga aagtgaatat cgacattttt
     1081 cttgatgctt ttataatttc tgcaacccct tatgatgagt tatataaacg ctatgatgat
     1141 ggaagttggg atagagcaaa atttgccgaa aagcatatcc ttttccagga acgaagtttg
     1201 gattatgatt atatgaaatt actatttgaa aaatcaatgt gcgtattaac gtaatccttt
     1261 atcacccaat caaaaaatta actatataag gatatgtgtg ctaatatcac tttcacatta
     1321 cagcaataat ccattacact atcttcacat cataatcctt gcgcaatgca ttctttacaa
     1381 attctacctg tggatagcca agtgctaaca cagcatgaac gcggttgtgt tctgaaatat
     1441 gtaatttttc ttttaaggaa ggggcgctat taagtgttgc agaagcatac cctataaaac
     1501 acgttccaag tcccattgag tgagcaacaa gcgctatatt gtaggctgca tactggccat
     1561 cctcaacagg catgcttcct tccatatcgc tgtgtacaat aattacgcaa ggggcaccat
     1621 gaaacaaaag gtcttttccc tcttttgctc tttcaagacc caatttgacg gactcatagt
     1681 tgtttttgta atactggaga attttttttc ctgcaaaaat aactgataga tatctgagta
     1741 tgggattgcc agctatggta ttaagcttaa taaaaaagtt ttttatatcc tcagccagca
     1801 ttaatacctt ttcccttcca ttaatgacaa caaattgcca gcgctgacaa ttactggccg
     1861 aaggtgcaag cacagcagct tcaatcaaat catggagcat ttctgtgttt actggatctg
     1921 ctttgaatct tcgtacactt cttcgtgagt gaataaagct tattatatca aatggtttta
     1981 tttcatcttt gatcactgcc gtatgaccaa atgaaaacac aggctgtttc aactcgttaa
     2041 aatgtatggc attaaattta catacgcaat agcaatgtga acacaaaatg cagtcatcgt
     2101 gcacaatgac aatttcattg tcttttattg ataatacctc ttttggacat atatctacgc
     2161 attttctaca caatgtacat ttatccttat caatgattcg accagtcaag aggtacc
//
LOCUS       DAOSZP010000391         2214 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_37529_length_2214_cov_2.866605, whole genome shotgun sequence.
ACCESSION   DAOSZP010000391 DAOSZP010000000
VERSION     DAOSZP010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2214
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37529_length_2214_cov_2.866605"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..469
                     /locus_tag="PK348_08430"
     CDS             <1..469
                     /locus_tag="PK348_08430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66277.1"
                     /translation="NEFTNAMKEYFQFTQIDYVTQQAESDLFQLGESGKAVKVDFQYL
                     EKNNRVYCKNTFFRFNDPQGRGAQGYNGNNFAFFCPIGTKPDPKSPGNKIPYFGMYYK
                     AMDGYSRLGETGTLMGATNGQHTQSKDQNTLWYKSQIASVHVGGINMVTATGV"
     gene            538..1482
                     /locus_tag="PK348_08435"
     CDS             538..1482
                     /locus_tag="PK348_08435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66278.1"
                     /translation="MLYVNGEQFTIPENQVKKYTAPHTWAIDPHKIRFDYANKRIQCP
                     DVGVPTEYRAFFAIGNESKRAHTVQYSRGTVPHPSRGNMTIHTPQMLMITDGYMLEGS
                     QTCTDQELNWFLTHAPWYGDGVAYNKGRKLICLYKRKNVAEKAETETELMWKMINKAM
                     GMKDNEIIAKAKGILKDAKIREKFFELREEVLENKASEEYPTEISLLKTGIIRFIQAY
                     PRHAQNIFQGKEVIALTTIEKARSMEVLYFDTEQPQEGFQGSWKFSPDPDKDPKFICT
                     ATTSERPLEALTNFLLSNKAGDMVNEIERACDKTLKSA"
     gene            1489..>2214
                     /locus_tag="PK348_08440"
     CDS             1489..>2214
                     /locus_tag="PK348_08440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66279.1"
                     /translation="MPQIPASTFSYTSILDKNSTPSKLIVQDTTDIAALITAGYSNFG
                     IFLRLTFTSVSGTITVYDNINGSVADIIYPATDNSNTIALPLQANGNLAFGTYSVTAQ
                     YQYLDEFGTDFSTEVSSAYEYTWTPPNAEISYRINLPASNITSIDVTNYGGQIVTMTR
                     VHTISPPGNAQLPPLNLPPISTSLATNVYQNITTGTWGVKIVSVVTYQFITTATGMNW
                     QVIDVVSGCTEFLVFSDVNLNALL"
BASE COUNT          772 a          462 c          436 g          544 t
ORIGIN      
        1 caatgagttc accaatgcaa tgaaagagta ctttcagttc acccaaattg actacgtaac
       61 tcaacaggca gaatcagacc tgttccaatt gggagaatca ggcaaagcgg taaaagtgga
      121 tttccaatac cttgaaaaaa acaaccgtgt atattgcaaa aataccttct tccgtttcaa
      181 cgatccgcag ggacgtggag cgcaagggta caacggaaac aactttgcat tcttctgtcc
      241 aattggaaca aaacctgacc caaaatcacc cggaaacaaa atcccatact ttggtatgta
      301 ttacaaagcg atggatggct actctcgttt gggtgaaaca ggaacattaa tgggcgcaac
      361 caatggtcag cacacacaat caaaagatca gaacaccttg tggtataagt cacaaatagc
      421 ttctgttcac gtgggcggca tcaacatggt tactgccaca ggagtataag gttcatgtag
      481 ttagcaatat agggctgcaa cagggcagcc ctttctttaa actttaaatt ataaaatatg
      541 ttatacgtca acggagagca gtttaccata cctgaaaatc aggtaaaaaa atacaccgcg
      601 ccccatacat gggctattga tcctcataag atcagatttg attacgccaa taaaagaata
      661 caatgccctg acgtgggtgt acctaccgaa tatagggctt tttttgcaat aggaaatgaa
      721 tcaaaaagag cgcatactgt tcagtattca agaggaactg taccgcatcc gagccgtgga
      781 aacatgacca ttcacactcc gcaaatgtta atgataacag atgggtatat gcttgaggga
      841 agccagacct gcacagatca ggaattaaat tggtttttaa ctcatgctcc gtggtatggt
      901 gatggcgtag cttataataa aggcagaaaa ttaatctgct tatacaaacg taagaacgtt
      961 gcagaaaaag ccgaaacaga aaccgagttg atgtggaaga tgattaacaa ggcaatggga
     1021 atgaaagaca atgaaatcat tgctaaagct aagggaatat taaaagacgc taaaattcgt
     1081 gaaaagtttt ttgaactaag agaagaagtt ttagaaaata aagcatcaga agaataccct
     1141 actgaaattt ctcttttaaa aacaggaatc atcagattta ttcaggcata cccaagacac
     1201 gcgcaaaaca tatttcaagg gaaagaagtc attgctttaa caactattga aaaagcaagg
     1261 tcaatggaag ttctttactt tgataccgag caaccacagg aaggatttca gggttcatgg
     1321 aaattcagtc ctgatcctga caaagaccct aaatttatct gcacagcaac aacaagcgaa
     1381 cgcccacttg aagcactcac caactttttg ttatcaaaca aagccggtga tatggtaaat
     1441 gaaatagaaa gagcgtgcga caaaacattg aaatccgcat aatgatagat gccacaaata
     1501 cccgcatcca cattttcgta cacgtcaata cttgacaaaa acagtacgcc aagcaagctg
     1561 attgtccaag acacaacgga cattgcagcg ttaataacgg cagggtatag caactttggc
     1621 atttttttaa gattaacctt tacctctgta tcaggaacca taacggtata tgataatata
     1681 aacgggtcag tagccgatat aatttatcct gctaccgaca actccaatac catagcacta
     1741 ccattacagg ctaatggaaa tttagctttt ggaacatatt ctgtaacagc gcaataccaa
     1801 tatttagacg agttcggaac cgacttctcc acagaagttt caagcgcgta tgaatataca
     1861 tggacaccgc caaatgctga aatatcctat cggataaacc ttccagcgag caacattacg
     1921 tccattgacg taactaatta cggaggtcag atagtaacca tgacaagggt gcatacgatc
     1981 tcaccgcccg gaaacgccca actacctccc ctaaatctcc ctccaatatc cacctctctt
     2041 gccaccaatg tttatcagaa tataactacg ggaacatggg gcgtaaaaat agtatcggta
     2101 gttacgtatc aatttataac tactgctacg ggaatgaatt ggcaggtgat agacgtggtt
     2161 tctggatgca cagaattttt agttttcagc gatgtaaacc tgaatgcgct tttg
//
LOCUS       DAOSZP010000392         2206 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_37709_length_2206_cov_3.772664, whole genome shotgun sequence.
ACCESSION   DAOSZP010000392 DAOSZP010000000
VERSION     DAOSZP010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37709_length_2206_cov_3.772664"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..762)
                     /locus_tag="PK348_08445"
     CDS             complement(<1..762)
                     /locus_tag="PK348_08445"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514551.1"
                     /note="catalyzes the phosphorylation and decarboxylation
                     of oxaloacetate to form phosphoenolpyruvate using GTP;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase"
                     /protein_id="HXK66280.1"
                     /translation="MKLTELKHKALKKWVEDAIALMQPEDVYVCDGSKAEYDRLAEQM
                     VAEGLAIRLNQKKRPGCLLFRSDPSDVARVENRTYISSKTKEAAGPTNNWIDPVELKQ
                     TMTDLYRGCMRGRTMFVIPFSMGPVGSPIAKIGVEITDSPYVVINMHIMTRVGAKVLD
                     VLGTDGEFVPCFHSVGKPLANGESDHGKWPCAPLEKKYISHFPETREIWSYGSGYGGN
                     ALLGKKCLALRIASVMARDEGWLAEHMLILKITNPE"
     gene            complement(937..1239)
                     /locus_tag="PK348_08450"
     CDS             complement(937..1239)
                     /locus_tag="PK348_08450"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HXK66281.1"
                     /translation="MIYAFNKTERGYFLKIEGDLDLYSSYNLKEDVKKAYLSAPDDLT
                     IDLSGLNYIDSAGIAFLIDIKKTSIQNGKKCEIQNIPGDILRMFRSLRVDTILQIQ"
     gene            complement(1311..>2206)
                     /locus_tag="PK348_08455"
     CDS             complement(1311..>2206)
                     /locus_tag="PK348_08455"
                     /inference="COORDINATES: protein motif:HMM:NF017026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HXK66282.1"
                     /translation="TYHKEQITTLGWELTVCNMLYPENSPCREVIKKNAPYGTLLCEF
                     LSSFIDMKQVKTILEAGGGYGFLMKDFLACCPHVQAIMVDISPAMLAEQKKNIRGNIS
                     FIHSDFLELDNALLSDIDLIICNEIIGDFITVCDITHDILESFTLDEIKECKRLINEY
                     NLEIEANPFNFNLGALLAIEKICKAGTKYIYISEHSCESELQGEFRECVPVRPTFNPY
                     EIRLFGHSEYTIRFSHLEAVAKKWGYTIHRGVYNDIFGCRMTPKLQFILNSTASAKDE
                     YEILRQFIGDMFTYEYVVCCK"
BASE COUNT          672 a          464 c          359 g          711 t
ORIGIN      
        1 ttccgggttg gttatcttca gtatcaacat atgttcggca agccatcctt catcacgcgc
       61 cataactgat gcaattctga gtgccagaca ctttttccct aaaagcgcat taccgccata
      121 gcctgaacca tacgaccata tttcacgagt ttcaggaaaa tgagaaatat attttttttc
      181 aagtggagca catggccatt tgccatggtc actttcacca tttgctaaag gtttacccac
      241 tgagtgaaag cagggaacaa attcaccatc agttcccaat acatcaagca cctttgcccc
      301 cacgcgggtc ataatatgca tattgataac aacatacggt gaatcagtaa tttccactcc
      361 aatttttgca ataggtgaac caacggggcc cattgagaaa ggaataacaa acatggtacg
      421 tccacgcata caaccacggt aaagatcggt catggtttgt tttaactcca caggatcgat
      481 ccagttgtta gtgggaccag ctgcttcctt cgtttttgaa gaaatatacg tcctgttttc
      541 aacacgtgca acatcacttg ggtctgagcg gaacaaaagg caaccaggtc gtttcttctg
      601 gtttaaacga atagccagcc cttcagcaac catttgctct gccagccggt catattcagc
      661 tttcgaacca tcgcatacat agacatcctc cggctgcatt aatgcaattg catcctcaac
      721 ccatttttta agtgctttgt gcttcagctc tgttaatttc atagcttatc ctttctattt
      781 cagtatacat tggggattat acataaattg acgttactgt ggtatggtat gacaattttg
      841 tcaacaattc tgttaagata catatatagc aaaaaatatt ttatttttca aatattaact
      901 gatgcaggat atgattgaca agaaaggttt tgtgtatcac tgtatttgta aaatagtatc
      961 aacccgtaga gaacgaaaca tcctcagaat atccccaggg atattctgta tttcacattt
     1021 tttaccattc tgaatactag ttttttttat atcaattaaa aaggcaatac cagccgagtc
     1081 aatatagttc aatcctgaaa gatcaattgt taaatcatca ggagcggata agtatgcctt
     1141 ttttacatct tcctttaaat tataggaaga atataaatca agatctcctt caattttgag
     1201 gaagtatccc cgttcagttt tattaaatgc atatatcata attgtaataa tatactgctg
     1261 ttctgatttt cggacatata tggctgcata ttgagtaaat tccactccat ttatttacaa
     1321 cacacaacgt attcataggt aaacatatca cctataaact gccgtaatat ttcatactca
     1381 tcctttgcac tggcagtgct gtttaaaata aactgtaatt ttggtgtcat acggcaacca
     1441 aatatatcat tatatacacc tctgtgaatg gtgtaccccc atttttttgc aacagcctca
     1501 agatgcgaaa agcgtatggt atattcgcta tgtccaaaca gccgtatttc atatggatta
     1561 aaggttggcc ttacaggcac acattccctg aattcgccct gtaattctga ttcacagcta
     1621 tgctcactta tatatatgta ttttgttcct gctttgcata ttttttctat cgcaagaagt
     1681 gcccctaaat tgaaattaaa tggattggct tcaatttcaa ggttatactc attaataagg
     1741 cgtttgcatt cttttatctc atctaatgta aaagattcca gtatgtcatg cgttatatca
     1801 cacactgtaa taaaatcccc tattatctca ttacatataa taagatcaat atcagatagc
     1861 aaggcattat ccagttccag aaaatctgaa tgaataaatg aaatattacc ccgtatgttt
     1921 ttcttttgtt ctgcaagcat tgcaggagat atatcaacca tgatagcttg tacatgggga
     1981 cagcaagcta aaaaatcctt cattaaaaag ccatatcccc caccagcttc cagtattgtt
     2041 ttaacctgtt tcatatcaat aaaactagaa agaaattcac acaacagcgt tccataggga
     2101 gcatttttct taattacttc cctgcagggg ctgttttctg gatagagcat attacaaacc
     2161 gtaagctccc accctaacgt ggttatttgc tctttatgat aggtgc
//
LOCUS       DAOSZP010000393         2205 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_37732_length_2205_cov_3.561395, whole genome shotgun sequence.
ACCESSION   DAOSZP010000393 DAOSZP010000000
VERSION     DAOSZP010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37732_length_2205_cov_3.561395"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1731)
                     /gene="leuS"
                     /locus_tag="PK348_08460"
     CDS             complement(<1..1731)
                     /gene="leuS"
                     /locus_tag="PK348_08460"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008411012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HXK66283.1"
                     /translation="MQEKEYKPKQIEKKWQDRWNQEKVFLSKRDKNRQKYYILEMFPY
                     PSGRIHMGHVRNYSIGDVVARYKRMQGFNVFHPMGWDAFGLPAENAAIKHNIPPKKWT
                     DDNIAYMKKQFKMLGYSYDWEREIATHTPEYYKWNQYVFIKMYEKGLAYKRKAPVNWC
                     PTCNTVLANEQVENGLCWRCETQVTQRELEQWFFKITDYAEDLLSGHDILKEGWPERV
                     IVMQKNWIGRSTGLVVNFKVADTGENFPIFTTRPDTIYGVTFMVIAPEHPFLERVKDS
                     KVKEFIKRIKEQSLVDRLSEEKEKEGIDTGIKIINPFNGDIVPLYVGNFVLMEYGTGA
                     IMAVPAHDTRDFAFAKKYGIPIKLVIDNPSSPIDVASMKDAYVEDGICVNSGPFTGMT
                     NKKAIEAISDYAEKEGIGYKKVNYRLKDWLISRQRYWGCPIPIVYCETCGAQPVPYDN
                     LPVALPTDVDFHGDSRSPLTMMPEFYETKCPHCGGKAKRETDTMDTFVDSSWYFAKFT
                     SPRSKEIFDFDEVNYWTPVDQYIGGIEHACMHLLYARFFTMVLNDLGILKYREPFTRL
                     LTQGMVIKDGA"
     gene            complement(1880..>2205)
                     /locus_tag="PK348_08465"
     CDS             complement(1880..>2205)
                     /locus_tag="PK348_08465"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HXK66284.1"
                     /translation="AETGLIVVDDNGGYTGILSLNEVRQALLSSDVVELLICQDIVLH
                     VNPCSENNSAAEALRRMDEYGIDFLPVAKGNKVTAIVLYRDIISMYNKIIVQIEEKQY
                     GHFGL"
BASE COUNT          650 a          496 c          347 g          712 t
ORIGIN      
        1 tgcgccatct tttattacca ttccctgtgt cagcaaacgg gtaaaaggct ctcggtattt
       61 caaaattccc agatcattca ggaccatggt aaaaaagcgt gcgtacagca aatgcataca
      121 ggcatgttct attccaccaa tatactggtc aactggagtc cagtaattga cctcatcaaa
      181 atcaaagatt tcctttgaac gaggagaagt aaactttgca aaataccacg aagagtcaac
      241 aaaagtatcc atggtatctg tttcacgctt tgctttcccc ccacagtgtg gacatttggt
      301 ttcataaaat tcaggcatca tggtaagtgg tgaacgagaa tctccatgaa aatcaacatc
      361 cgttggcaat gctaccggta agttatcata tggtacaggt tgagcaccac aggtttcaca
      421 atacacaatt ggtattgggc agccccagta ccgttgccga gatattaacc agtcctttag
      481 gcgataattt acttttttat aaccaatacc ttctttttca gcatagtctg atattgcttc
      541 aatggccttt ttattagtca tacctgtaaa gggaccggaa ttaacacata taccatcctc
      601 aacatacgca tctttcattg atgcaacatc aataggcgat gaaggattat caataacaag
      661 ctttatagga atgccatatt ttttagcaaa agcaaagtca cgagtatcat gtgccgggac
      721 agccataata gcacctgtac catattccat gagaacaaaa ttaccaacat ataaaggcac
      781 aatatcaccg ttaaacggat tgataatttt tattccggta tctatacctt ctttttcctt
      841 ttcttcagaa agtctgtcta ccagagattg ctctttaatt cttttaataa attcctttac
      901 ttttgagtct tttactcgct ccagaaatgg atgttcggga gctattacca taaaagtcac
      961 accgtaaata gtatctggac gcgtggtaaa aattgggaag ttttcaccgg tatcagcaac
     1021 cttaaaattt acaaccagtc ctgttgatct gcctatccag tttttttgca tcacaataac
     1081 acgctcgggc cacccttctt tcagaatatc atgccctgat aacagatcct cagcataatc
     1141 ggttatttta aaaaaccatt gctcaagttc acgctgggtt acctgcgttt cacatcgcca
     1201 gcataggcca ttttcaacct gttcattggc aagcactgta ttacatgttg ggcaccagtt
     1261 aaccggtgct ttgcgcttat aggccaatcc tttttcatac atcttgataa aaacatactg
     1321 attccattta taatactcag gcgtatgggt tgctatctcc ctttcccaat cataagagta
     1381 ccccaacatc ttaaattgct ttttcatata cgcaatattg tcatccgtcc attttttagg
     1441 aggaatattg tgcttgatag cagcattttc tgctggtaag ccaaatgcat cccatcccat
     1501 gggatggaat acattaaagc cctgcattcg tttataacga gctacaacat ctccaattga
     1561 ataattgcgc acatggccca tgtgtattct gccagatgga tacggaaaca tttctaatat
     1621 gtaatacttt tgcctgtttt tatcacgttt tgataaaaaa accttttctt gattccatcg
     1681 gtcctgccac tttttttcaa tttgcttggg tttatattcc ttctcctgca tacatcatcc
     1741 tccaataata cattttacaa cagttgcacg caatacatca atagtatcta tcaatactta
     1801 ttaattcaat tcatatgcac attattcacg ctggttttat cgtcaagtat tttgttacca
     1861 ggtaactatc gcacatatat tataaaccaa aatgtccata ttgcttttct tcaatctgca
     1921 ctataatttt attgtacata ctgattatat cccgatacag gacaatagct gtaactttat
     1981 ttccttttgc tacaggtaaa aaatcgattc catattcatc cattcttctt agcgcctctg
     2041 ctgctgaatt attctcgctg cagggattaa catgtaacac tatatcctga cagatgagta
     2101 attcaaccac atcactggaa agaagtgcct gcctgacttc atttaatgac aatatacctg
     2161 tatagccacc attatcatca actacaataa gacctgtttc cgctt
//
LOCUS       DAOSZP010000394         2203 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_37767_length_2203_cov_3.840782, whole genome shotgun sequence.
ACCESSION   DAOSZP010000394 DAOSZP010000000
VERSION     DAOSZP010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2203
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37767_length_2203_cov_3.840782"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..626)
                     /gene="pyrF"
                     /locus_tag="PK348_08470"
     CDS             complement(<1..626)
                     /gene="pyrF"
                     /locus_tag="PK348_08470"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR02127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HXK66285.1"
                     /translation="MNYCESLIYNSRKHKSIVCMGLDPVLEDIPIRKDSIRNTIVTFY
                     EGILNKCLQHHIYPACVKPNYAFYAQYGIEGLHALCDVITLFGNEGLPVILDVKRGDI
                     GKTAMAYAKEAFEFFKADAVTLSPYLGFDSIQPFTSNYPDKGYYILVKTSNKSSSEIQ
                     DIVVNNEPLYLLIAKKLIEWYAPGIGAVVGATFPQQLNNIITVFEESG"
     gene            complement(627..1163)
                     /locus_tag="PK348_08475"
     CDS             complement(627..1163)
                     /locus_tag="PK348_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013075028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HXK66286.1"
                     /translation="MAVDKRTILKHIHIQKGDITKMEVDAIVNAANPSLLGGGGVDGA
                     IHRAAGPQLLEECRTLGGAQPGEAKITKGYRLKAKYVIHTPGPVYRGGSHNEAAILRN
                     SYYNSMRLAKDNGCTSIAFPAISTGVYGYPKEEACSIALSTIIEFMEKENFLIDVYFV
                     LFDDDNYAIYTKHLQELS"
     gene            1301..>2203
                     /locus_tag="PK348_08480"
     CDS             1301..>2203
                     /locus_tag="PK348_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXK66287.1"
                     /translation="MIRYFDIIVIGAGHAGAEAALASARRGFATLLLTGNLDTICQMS
                     CNPAIGGLAKGHLVKEIDALGGEMGLAIDETGIHYKMLNKSKGPAVWAPRAQADKKQY
                     QLRMKEVLEKQKNLTVIQDIVDAIIVEGGQVQGVETIRGQQHVAKAVIVCTGTFLKGL
                     IHIGNYNEKAGRLGDFSAEKLSDSLRNLGFPVKRLKTGTPPRVNGNSIDFGKCEVQHP
                     DPVPVPFSYRTHSINRQQVPCYITYTTEKTHAIITNNLHLSPLYSGKIQGIGPRYCPS
                     IEDKVVRFSSRNRHQLFLEPEGLHT"
BASE COUNT          667 a          414 c          449 g          673 t
ORIGIN      
        1 tttccggatt cttcaaacac tgtgataata ttattgagtt gctgaggaaa tgttgcacct
       61 actacagcac caatacctgg agcataccac tcaataagtt tttttgcaat taataaataa
      121 agcggttcat tgttgacaac aatatcctgt atttctgaag agcttttatt ggaggtttta
      181 acaagaatat agtatccttt gtcaggatag ttgctggtaa atggctgtat tgaatcaaat
      241 cctaaatatg gtgaaagggt tacagcatct gctttaaaga attcaaaggc ttcttttgca
      301 taggccatag cagtcttccc aatgtcacct cgctttacat caagaatcac tgggagtcct
      361 tcattcccaa ataacgtaat gacatcacat aaggcatgca aaccttcaat gccgtactgt
      421 gcataaaaag cgtaatttgg ctttacacat gcggggtaaa tatggtgttg caaacatttg
      481 ttaagtatac cctcgtagaa ggttacaata gtattgcgta tagaatcctt tctaatggga
      541 atatcttcaa gtaccgggtc aagccccata cacacaatac ttttatgttt cctggaattg
      601 tatatcagcg attcacaata gttcatttac gaaagctcct gtaaatgttt tgtatagatt
      661 gcataattgt catcatcaaa aagcacaaaa tacacgtcta ttaaaaaatt ttctttttcc
      721 atgaactcaa tgatagttga taacgctata ctacatgctt cttcttttgg gtatccatac
      781 acaccggtgg aaatagctgg aaatgcaatg gatgtacagc cattgtcctt tgcaagtctc
      841 atgctgttat aataggaatt tcttaatata gcagcttcat tgtgacttcc tccacggtac
      901 actgggccag gtgtatgaat gacatattta gctttaagcc tgtagccttt tgtaattttg
      961 gcttctccgg gttgagcacc acccaatgtc ctgcattctt ctaatagctg tgggccggcg
     1021 gcacggtgta ttgcgccatc aacaccacct ccgccaagca atgaaggatt tgccgcatta
     1081 actatcgcat caacttccat ttttgttata tcacccttct ggatatggat atgttttaaa
     1141 atggtacgtt tatctacagc cattggtacc cccactgtgt aatatagtaa aaaaattttt
     1201 ttgcatggta aaaaaaggta ttattaattg ataatagtac tcacactcta tgtttaattc
     1261 aagcatattt attgaaaatg tttaagagat atatactaca atgatacggt attttgatat
     1321 tattgttatt ggtgcaggac atgcaggagc tgaggcagca ctggcatcgg cacgccgtgg
     1381 atttgcaacg ttattgttga caggcaacct tgatactatc tgtcagatga gttgtaaccc
     1441 tgccataggt gggctggcaa aaggacattt agtgaaagag attgatgccc tgggtgggga
     1501 aatgggtctt gcaatagatg agacaggtat tcattataag atgctcaata aaagcaaagg
     1561 tcctgcagtg tgggcgccgc gggctcaagc agataaaaag cagtatcaat tacgcatgaa
     1621 agaagttctt gaaaaacaga agaatttaac tgttattcag gatatagttg atgcaattat
     1681 tgtagaaggt gggcaggtac agggtgttga aacaatacgc ggacagcagc atgtagctaa
     1741 agcggtgatt gtatgtacag gtacattttt gaaagggctt atccatattg gtaattataa
     1801 tgaaaaggcg ggcaggttgg gtgatttctc ggctgagaag ctttcagatt cactacgtaa
     1861 ccttggattt cctgttaagc ggttaaagac aggaacacct ccccgggtaa atggtaattc
     1921 aatagatttt ggaaagtgtg aagtacagca tcctgatccg gtaccggtac cattttcata
     1981 tagaacacat tccattaatc gtcaacaggt accctgctat attacctata caactgaaaa
     2041 aacacatgca attattacca ataatctaca tctttctcca ttatattcag gcaagataca
     2101 ggggatagga ccacggtatt gtccctcaat tgaggataag gttgtacggt tttcttcacg
     2161 caacaggcat caactctttt tagaaccaga aggtttgcac acc
//
LOCUS       DAOSZP010000395         2199 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_37846_length_2199_cov_3.356343, whole genome shotgun sequence.
ACCESSION   DAOSZP010000395 DAOSZP010000000
VERSION     DAOSZP010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2199)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2199)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2199
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37846_length_2199_cov_3.356343"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..469)
                     /locus_tag="PK348_08485"
     CDS             complement(<1..469)
                     /locus_tag="PK348_08485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66288.1"
                     /translation="MMKKIAIALIICAIVISKGFAEESKPLVNISGVGYFDWKKDIQK
                     SDGTESENTFELNRVYLNFTKQFDEIWSMRVTTDIGQVESESTKPYQEDAATTSEVKS
                     KTNNYTVYLKFTYIQAAQQYEHLSYTLQFGMVGTPLLSYIDGQSDYRWIQQNYF"
     gene            complement(480..>1134)
                     /locus_tag="PK348_08490"
     CDS             complement(480..>1134)
                     /locus_tag="PK348_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011188188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="permease"
                     /protein_id="HXK66289.1"
                     /translation="NEGIWYTIFSYKWEITGISSLLFGLTLGAWFGVKWWKVLVSIIV
                     VGVLGFLFQHEPMIPFAAGVVALTLITATEKGEMTTWLEQTWSFAKQILPLLLGGVLV
                     AGFLLGRPGHEGIIPSEWVSVAVGGNSVFANFFSSLVGAFMYFATLTEVPILQGLLGS
                     GMGYGPALALLLAGPALSLPNMLVIRSVIGTKKTVIYVSLVVIMATITGLIFGMLYQ"
     assembly_gap    1135..1144
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1145..1783)
                     /locus_tag="PK348_08495"
     CDS             complement(<1145..1783)
                     /locus_tag="PK348_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014260230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HXK66290.1"
                     /translation="MDWKSELKKLLYIVAAFLIFYYLPIGNPRFDNAVFEALHLTKWY
                     AQEHVLLCLVPAFFIAGAIAAFVSQNSVMKYLGPTAPKLLAYGVASVSGTILAVCSCT
                     VLPLFAGIYTMGAGLGPATAFLYSGPAINVLAIILTARVLGLEIGVARAVGAILFSVV
                     IGALMYLIFRKEEIEKANAMMNMPEPETPRPLWQTVVYFFLMVAILVFANWGK"
     gene            complement(1860..2162)
                     /locus_tag="PK348_08500"
     CDS             complement(1860..2162)
                     /locus_tag="PK348_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015414442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HXK66291.1"
                     /translation="MKALAHPSRLMMVEELEKGPRCVCELQELVGADMSTVSKHLAVM
                     KNAGLVYDVKQGTTVYYHLKTSCVLDFIACVDRVIEHNVAHQVKIARCCVRSVRHS"
BASE COUNT          683 a          539 c          374 g          593 t
ORIGIN      
        1 caaaatagtt ctgctgtatc caccggtaat cgctctggcc atcaatgtag ctaagaagtg
       61 gtgtgccaac catgccaaac tgcagcgtat acgataggtg ctcatactgc tgtgctgcct
      121 gaatatacgt aaatttaaga tacactgtat aattgttggt ttttgattta acttctgaag
      181 tggttgcagc gtcttcctga tatggttttg tactttcact ttctacctgg ccaatgtcag
      241 tagttacacg catgctccat atttcatcaa actgctttgt gaagttgaga tacacacggt
      301 tgagttcaaa tgtattttcg ctttctgttc catctgattt ctgtatatcc ttcttccagt
      361 caaaataacc aacacccgaa atattcacca gcggtttact ttcctctgca aaacctttac
      421 tgataactat cgcacatata atcaatgcta ttgcaatttt cttcatcatc aatttcccct
      481 tattgatata acattccaaa aataagtccc gtgatcgttg ccatgatcac aaccagcgac
      541 acataaataa cagtcttttt tgtaccaatg acactgcgta ttacaagcat attgggaagc
      601 gataacgctg gccctgctaa tagaagtgcc agtgcaggac catatcccat accactacca
      661 agtaatccct gtaaaatagg aacctcggtg agtgttgcaa aatacatgaa tgcaccaacc
      721 agtgaagaga agaagtttgc aaatacagaa ttaccaccaa cagcaacact tacccactct
      781 gacgggatga ttccctcatg tcctggacga cccaacaaaa atccagcaac aagtacacca
      841 cctaaaagaa gcggtagtat ctgttttgca aatgaccatg tctgctcaag ccaggtggtc
      901 atttcacctt tttcagttgc agtaataagt gtcagtgcaa caactcctgc agcaaatggt
      961 atcatgggct catgctgaaa aagaaatccc aaaacaccaa cgactatgat agaaaccaga
     1021 accttccacc atttaacacc aaaccatgca ccaagcgtta gcccaaataa caacgatgat
     1081 atgccggtga tttcccattt atatgaaaaa atagtatacc atataccttc atttnnnnnn
     1141 nnnnctttcc ccagtttgca aataccagta tcgccaccat caaaaagaaa tagactaccg
     1201 tttgccacag tggacgcgga gtttcaggct caggcatatt catcatggca ttggcttttt
     1261 ctatttcctc tttcctgaat atcaggtaca tcaatgcacc aataacaaca ctgaataaaa
     1321 ttgcacctac agcccgtgca acaccaattt caagtcccag gacacgggca gttaaaataa
     1381 ttgccagaac attgatcgca ggccctgaat ataaaaaggc tgttgccggt cccagtcctg
     1441 ctcccatggt atagatacct gcaaaaagcg gcaacaccgt acatgaacac acagcaagta
     1501 ttgtaccgga aaccgatgcc acaccatacg ccagtaattt tggcgcagta ggccctaaat
     1561 atttcataac actattctgg cttacaaatg cagcgatagc tcctgcaata aaaaacgcag
     1621 gcaccaggca caaaagaaca tgttcctgtg cataccactt tgtcagatgc aacgcttcaa
     1681 acacagcatt gtcaaaacgc ggattgccaa ttggcagata gtaaaaaata agaaatgctg
     1741 caacaatata cagcaacttt tttaattcac tcttccaatc cataaataca tctccttcta
     1801 tattgtatgt tacataaatt aagctgtcat aatatttgta gcgtacatga atggtaattc
     1861 tatgaatgcc gtacactccg aacacagcag cgagcaatct taacctgatg tgccacattg
     1921 tgttcaataa ccctatcaac acacgcaata aagtcaagaa cacaggaagt cttaaggtga
     1981 taatatacgg tggtaccctg ttttacatca tacaccagcc ctgcattttt catcaccgct
     2041 aagtgtttgg acaccgttga catgtcagca ccaacaagct cttgaagctc gcatacgcat
     2101 cgaggtcctt tttcaagttc ctctaccatc atgagcctgc ttggatgcgc cagcgctttc
     2161 atgatagcag cacgtgtttc atacttttgc tttactaat
//
LOCUS       DAOSZP010000396         2197 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_37902_length_2197_cov_2.323063, whole genome shotgun sequence.
ACCESSION   DAOSZP010000396 DAOSZP010000000
VERSION     DAOSZP010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37902_length_2197_cov_2.323063"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            49..972
                     /locus_tag="PK348_08505"
     CDS             49..972
                     /locus_tag="PK348_08505"
                     /inference="COORDINATES: protein motif:HMM:TIGR01777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HXK66292.1"
                     /translation="MNTIIITGGTGFIGSNLTQKLLTKGYSVVILTRNPDRFLSHTPA
                     LTYAGWDGKTADGWGNVVNEAKAIINLAGESIASTRWTNEKKTKILQSRINAGNAIID
                     AIKKAKHYPEVLIQASAIGYYGSRSESLTETSSPGLGFLPDVAIQWEASTKEAIKYGI
                     RHVIIRTGLVLGENDGFLKILIPVFKWYLGGYFGDGSQWMSWIHIEDEVNAIIHCLEN
                     DAIKGPVNAVSPNPVQAKYFYKTLASVLRRPCLFKIPEFAIRLILGQMAEELILSSQK
                     VHPDVLISTKFNFTYANLYEALYSLLSKRSH"
     gene            1003..>2197
                     /locus_tag="PK348_08510"
     CDS             1003..>2197
                     /locus_tag="PK348_08510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66293.1"
                     /translation="MNNLPVITYDEAQMLEWHALLSYFRDFCSTETGKQYCINLQPLQ
                     YQDIIIQYNKINCLIEILRVGHSPDFSGIYDITPLLDKAAKGGTLLLDEIFKIRLFLV
                     AQHRITSFFRSVHYDISVLTELHAIDECIQLFRLLTTSLTENCNLNENTYPALRKIQK
                     DINEMRSIIEKNLTGIMHSQAFQHVLQEKIFTVKNNRYCIPVKSSHKGKMKGTVLDIS
                     SSGATLFIEPESIHELNNTLLYKEVELQREIDKILQILSSEIGTYAHELKHNATILAY
                     LDFCVACARFAITYNAHTPAVSPAPLLDCINARHPLLAILLRDAVVPCTIACGKSFNC
                     LIISGVNTGGKTVLLKMLGLFALMVRHGFPITANPDSTIGYFEHIFVDIGDEQNIMQS
                     LSTFSG"
BASE COUNT          722 a          393 c          402 g          680 t
ORIGIN      
        1 acggcaattg ggggacaaca attgtcattt tgaaaattgg agaataaaat gaatacaata
       61 attatcaccg gaggcacagg atttattgga tcaaatctga cacaaaaact gctgacaaag
      121 ggatattcag ttgtaatatt aacccgtaac cctgatagat ttttatcaca caccccagca
      181 ttgacatatg ctggatggga tggaaaaact gctgatggtt ggggtaatgt tgttaatgaa
      241 gctaaagcta ttataaattt ggccggtgaa tcaatcgcaa gtaccagatg gactaatgaa
      301 aagaaaacta aaatattaca gagtcgaatc aatgcaggca atgccatcat tgatgcaatt
      361 aaaaaagcaa aacactatcc tgaagtactg atacaggctt ctgctattgg ctactacggt
      421 tcacgcagtg aatcattgac tgaaacatcc agcccgggac tgggattttt gcctgatgtt
      481 gccatacaat gggaagcatc aacaaaagaa gccataaagt atggcatacg ccatgtcata
      541 ataaggacag gtttggtgct tggagaaaat gatggatttt taaaaatatt gattccggta
      601 ttcaagtggt atctgggtgg ttatttcggt gatggctcac agtggatgtc ctggattcat
      661 attgaagatg aagttaatgc cattatacac tgtttggaaa acgatgcaat caaagggcct
      721 gttaatgctg ttagcccaaa tcctgttcag gcaaaatatt tttataaaac attagcaagt
      781 gttttacgca ggccatgttt atttaaaata cctgaatttg ctattagatt gatattgggg
      841 caaatggcgg aagaattgat tttatccagt caaaaagtcc atcctgatgt gctgatatcc
      901 acaaaattta actttacgta tgctaatctt tatgaagcat tgtattcttt attatctaaa
      961 agatcacatt aatacacctg actgaataaa ccattctgca acatgaataa tttaccggtg
     1021 attacatatg atgaagctca gatgcttgaa tggcatgccc ttttatcata cttcagggat
     1081 ttttgctcta ctgaaactgg aaaacagtat tgtataaatt tacaacccct ccagtaccag
     1141 gatataatta tacagtataa taaaattaac tgcttaatag aaattttacg tgtagggcat
     1201 tctcctgatt tttccggtat atatgatata acaccgttac ttgacaaagc agcaaaaggt
     1261 ggaacccttt tattagatga aatttttaaa attcgtttgt tcctggttgc gcaacacagg
     1321 attacatctt tttttagatc agtacactat gatatatcgg ttcttacaga acttcatgca
     1381 attgatgaat gcatacaact tttcagactg ttgacaactt cattaaccga gaattgcaat
     1441 cttaatgaaa acacctaccc tgctttacga aaaatacaaa aagatattaa cgaaatgcgt
     1501 agtataatag aaaagaatct tactggtatc atgcattcac aagcttttca gcacgtttta
     1561 caggaaaaga tattcacagt aaaaaataat agatattgta tcccggttaa atcatcacac
     1621 aaagggaaaa tgaaaggtac tgttttagat atttcttctt caggtgcaac tttattcatt
     1681 gaacctgaaa gcattcatga attgaacaat acattgttat ataaagaagt agaactgcag
     1741 cgtgaaatag ataaaatact ccagattcta tcttcagaaa ttggtaccta tgcgcacgag
     1801 ttaaaacata atgcaaccat tttagcctat ctggatttct gcgtagcctg tgcacgtttt
     1861 gccattactt acaatgccca tacacctgct gtttcacctg ctcccctttt agactgcatt
     1921 aatgccagac atccactgct tgctattcta ttacgtgatg ccgtagtacc ctgcactata
     1981 gcttgtggta aatcattcaa ctgccttatc atatctggtg taaatactgg tggcaaaaca
     2041 gttctgctta aaatgttagg attgtttgct cttatggtac gtcatggctt cccaattaca
     2101 gctaaccctg attcaaccat tgggtatttt gaacatatat ttgttgatat tggtgatgaa
     2161 cagaatatta tgcaatcgct atccactttc agcggtc
//
LOCUS       DAOSZP010000397         2195 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_37942_length_2195_cov_3.018692, whole genome shotgun sequence.
ACCESSION   DAOSZP010000397 DAOSZP010000000
VERSION     DAOSZP010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2195
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37942_length_2195_cov_3.018692"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(59..1051)
                     /locus_tag="PK348_08515"
     CDS             complement(59..1051)
                     /locus_tag="PK348_08515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66294.1"
                     /translation="MDKNGQLTFFDDPLLQGINEAYTFIEAGDFTQAVKKIDDLMTIN
                     PDYPGLAEAYRVARFWESRKKAMQELQKGKDTADFLMTQWEEFEAFAQNRGLVWATAY
                     QKAMRFVFFTASEHYTIAFNDRENPTDNLQLLMQLGTCFIRLGEYQHTIEALEYARSA
                     YRSNACILSLLAESYYHTGDVPRALLYFREAFLLNPSEIDIQLLSAKPIIEALELVKQ
                     HKPDCSDVREWVPVMAHIHDIFYVKRQLNSSQLETIKKDIVKLEASFQTLDKEKIAQS
                     NIVPRLINRYLWMLDYFEFQQYDQKSLFDIHDRLLAIDKGLFESYFQKKTFSKH"
     gene            complement(1054..>2195)
                     /locus_tag="PK348_08520"
     CDS             complement(1054..>2195)
                     /locus_tag="PK348_08520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66295.1"
                     /translation="TIIFGQESKNHALARNLLIINIILALGVLFIFLKPVFGITSIHR
                     WLLVSLLILIQISSIGYYLIYVMMHSFKKIVLPLQQQKETYQIKLETLLNQNGISLDG
                     ITIDEIYEFLLQYFEEYNEYNEKQIDLLTIQKEIDESTSIDILAEEKRKLESNVKMLS
                     DELHNLQEDIGIAIESAEHCMTIKMSLHNELDEKKALLVESQNKLDNIPEEIIDYEDE
                     KNSILQEIDDLEKELKTINEKDRLINIIISSFDTAMQRYFKDRFNILTGKIKMMLQDL
                     TQNKFGKIDEEYIKKIITHSVNTPNPSLKYCIYVAAIFALYELLQQELPNIHLPLIID
                     DPFVLMDEETMGEFIKILQLWVGNRQVIIFTHNSNIKKLCAVTEL"
BASE COUNT          663 a          427 c          338 g          767 t
ORIGIN      
        1 tgaagaacaa aattccctgg aaactattgg gttctaaatt aattagcgct ttaaaatatc
       61 agtgctttga aaaagtcttt ttttgaaagt agctttcaaa aagaccttta tcaatggcaa
      121 gcagtcggtc gtggatatca aaaagacttt tttgatcgta ttgctgaaat tcaaaataat
      181 caagcatcca taaatagcga tttataaggc gtggcacaat atttgattgt gctattttct
      241 ctttatccag cgtttgaaaa cttgcctcta atttcacaat atcttttttt atggtttcaa
      301 gctgtgatga attgagctgt cgcttaacat aaaaaatatc atgaatatgt gccataaccg
      361 gaacccattc ccgcacatca gagcaatcag gtttatgttg cttaaccagt tctaacgctt
      421 caataatagg tttggccgat agtaactgga tatcaatctc agacggatta agcaggaacg
      481 cctccctgaa ataaagtaat gcccgcggca catctccagt atgataatag ctttcagcta
      541 aaagtgataa tatacatgcg ttgcttcggt aggcactgcg agcatactct aatgcttcaa
      601 tagtatgctg atactcgccc aacctaataa aacaagttcc cagttgcata agaagctgca
      661 gattatcagt tgggttttcc ctgtcattga atgcaatagt gtaatgctca gaagcagtga
      721 aaaaaacaaa ccgcattgct ttttgatatg ctgttgccca aacaagcccc ctgttttgcg
      781 caaaagcttc aaactcctcc cactgggtca taagaaaatc tgctgtgtcc ttcccttttt
      841 gaagttcctg cattgccttt ttccttgatt cccagaagcg cgcaaccctg tatgcttctg
      901 ccagtcccgg gtaatcggga tttatggtca taaggtcatc tatctttttt actgcctgag
      961 tgaaatcacc tgcttcaata aatgtatacg cttcattaat accctgaagc aaaggatcat
     1021 caaaaaatgt taactgtcca tttttatcca taattacaat tcagtaactg cacacaattt
     1081 ttttatattg gaattatgag tgaaaataat cacctgcctg ttgccgaccc acagttgtaa
     1141 tattttgata aactcaccca ttgtctcttc atccataagc acaaatggat catcaataat
     1201 taatggcaga tgaatgtttg gtaattcctg ctggagtaat tcatacagag caaagattgc
     1261 agcaacatat atacagtatt ttaatgaggg gttgggcgta ttaactgaat gtgttattat
     1321 tttctttata tattcctcat caatttttcc aaatttattt tgtgtaaggt cctgtagcat
     1381 catttttatt tttcctgtta atatattaaa tctatccttg aaataacgct gcattgctgt
     1441 atcaaatgaa gatataataa tattaatgag acgatctttt tcatttatag ttttcagctc
     1501 tttttccaga tcatctatct cctgtaatat actattcttc tcatcttcat aatcaataat
     1561 ctcctcaggt atattatcca gcttgttctg tgattcaacc agtaaagctt ttttttcatc
     1621 cagttcattg tgcaaactca tttttattgt catacaatgc tctgcagatt ctattgctat
     1681 tccaatatct tcttgcaggt tatgaagctc gtcactcaac atctttacat tgctttcaag
     1741 ctttcttttt tcctctgcaa gaatatcaat tgaagtactc tcatcaattt ctttctgtat
     1801 cgtcaaaagg tctatctgct tttcattata ttcattatat tcttcaaaat actgcaacag
     1861 aaattcatat atttcatcga tggttatccc atccagtgat atgccattct gattcagcaa
     1921 tgtctctaat ttaatctgat atgtttcttt ttgttgctgt aacggcaaga ctattttttt
     1981 aaacgaatgc atcattacat aaataaggta atatcctata cttgatatct gaatcaaaat
     2041 taataaactt accagtagcc agcgatgtat agaggttatc ccaaaaactg gttttaaaaa
     2101 tataaatagc acccctaatg caagtataat attaataatg agcaggttac gtgcaagtgc
     2161 atgatttttg ctttcctgtc caaaaataat agttt
//
LOCUS       DAOSZP010000398         2194 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_37961_length_2194_cov_3.526414, whole genome shotgun sequence.
ACCESSION   DAOSZP010000398 DAOSZP010000000
VERSION     DAOSZP010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2194
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37961_length_2194_cov_3.526414"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..236)
                     /locus_tag="PK348_08525"
     CDS             complement(<1..236)
                     /locus_tag="PK348_08525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66296.1"
                     /translation="MTIDEAINLNKPIIIKAYSVSESIEHYIKLVLAKILQKHNCEVL
                     LIPLYTSVKELVVNAVKANHKFIYFEGKKSYESV"
     gene            complement(303..707)
                     /locus_tag="PK348_08530"
     CDS             complement(303..707)
                     /locus_tag="PK348_08530"
                     /inference="COORDINATES: protein motif:HMM:NF024087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HXK66297.1"
                     /translation="MNKKDLASPCGIFCAACPRFYKYGICKGCRADTFHDACEIYDCC
                     VRIGGMEFCFECDLFPCQRLKTFSNYHPGENFAHYRHIVIDNLLKIKEIGVENWYVIM
                     NQKFMQGEYGIQQRNPDGSFDISCCPCCNTTK"
     gene            980..1477
                     /locus_tag="PK348_08535"
     CDS             980..1477
                     /locus_tag="PK348_08535"
                     /inference="COORDINATES: protein motif:HMM:NF024498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin fold domain-containing protein"
                     /protein_id="HXK66298.1"
                     /translation="MKKRIPYVLAIITVLLGTYTIYSQIKWYDFNSGFAAVKNSKKPA
                     VIDFYADWCIWCKTMDKETFSNPAVEKKLSRDYIAIRINVEKNDSITFENKTYSAQEF
                     AAYCGVEGLPTLVFMDKEGKLVTRIPGYIKAEVFLSLLDYMKDECYKKKVSFHEYMQQ
                     KDCGK"
     gene            1488..1895
                     /locus_tag="PK348_08540"
     CDS             1488..1895
                     /locus_tag="PK348_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014198679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HXK66299.1"
                     /translation="MEFIEWGEHLSVGVTVFDNEHKQLVSLVNKLNHALLAGSAKKTM
                     EEILQNLVNYTKIHFKHEEDYMVLYDYPEYEKHKKEHDALTDQVMDFYMRYQAGKTVF
                     SLELMNFLKDWLTNHILISDKKYKNFFQSKNVE"
     gene            complement(1896..>2194)
                     /locus_tag="PK348_08545"
     CDS             complement(1896..>2194)
                     /locus_tag="PK348_08545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66300.1"
                     /translation="IRTIMKEISHTNTLIQDVNVSIRAIANLNTQIYNAIETIETSTS
                     EQRIANEEASKTIVYVSDAAQNLTDIVNTVSNVASAIKDIAQNLYTLVQAMTKA"
BASE COUNT          736 a          353 c          413 g          692 t
ORIGIN      
        1 aacaccgatt catacgattt tttgccttca aaatatataa acttatggtt ggcttttaca
       61 gcattgacca ccagttcctt cacagaagtg tacagtggga taagaagtac ctcgcaattg
      121 tgtttttgta aaatcttggc cagaaccagc ttgatataat gttcaataga ttcgctgacc
      181 gaatatgctt ttataataat aggtttgttc aggtttattg cttcatcgat agtcatggtt
      241 aatccctttt aatccgctat cgataaaata acactgtaat ggcagtatgt caagtatttt
      301 tattatttag ttgtgttaca acagggacag catgaaatgt caaaagaacc atcgggattt
      361 ctttgctgaa tcccatattc accctgcatg aatttctgat tcattataac gtaccagttt
      421 tcaacgccaa tttctttaat tttcaaaaga ttatctatca ctatatggcg gtaatgtgca
      481 aagttttcac cgggatgata gttactgaat gttttaaggc gctggcaggg gaaaaggtcg
      541 cattcaaaac aaaattccat cccaccaatg cgaacacagc aatcgtaaat ttcacaggca
      601 tcatggaatg tatcagcccg gcatcctttg caaataccat atttgtaaaa ccgggggcat
      661 gcagcacaaa aaattccaca cggagatgca agatcttttt tattcatacc attgatagtt
      721 aatttttatt aataaaagag tatcattttt tttagtcttt gcaaaaaaat tgctacacta
      781 atttaagaag aatctgtaat acttatataa ttatgtgcgg cgatagttac tggtaaacca
      841 tggtgcagtg taagccaatt tcagcaatgt tagcacagtc atatgtattt cacacatatc
      901 aaaacttcgt catcccacgt tgtgatttct ccagatgacg gtgtgtattg aatacaatgt
      961 aaaaggaggc attgatataa tgaaaaaaag aatcccgtat gtattagcaa ttattacagt
     1021 gctgttaggc acgtatacca tctatagcca gataaagtgg tatgatttca actctggctt
     1081 tgcagcggta aaaaattcaa aaaagccagc agtcattgat ttttatgcag actggtgtat
     1141 ctggtgcaaa actatggata aggaaacgtt ttccaatcct gcagttgaaa aaaaattaag
     1201 cagagattat atcgcaatac gcataaatgt tgaaaagaat gatagcatta catttgaaaa
     1261 taaaacctat agtgcacagg aatttgccgc atattgcgga gttgaaggcc ttccaacact
     1321 ggtattcatg gataaggagg ggaagctggt aacccgaata cctggttata taaaggctga
     1381 ggtattctta tcattgcttg attatatgaa agatgaatgt tacaaaaaga aagtatcgtt
     1441 tcatgaatat atgcagcaaa aagactgtgg gaaatagtga ggtatagatg gaatttatag
     1501 aatggggtga acatctaagt gtaggcgtta cagtttttga caatgaacac aaacagctgg
     1561 tttcattggt taataaatta aatcatgcct tgcttgcagg cagcgcaaag aaaacaatgg
     1621 aagaaatatt gcaaaatctg gtaaattata caaaaattca ttttaagcat gaagaagatt
     1681 atatggtact ctatgattat ccagaatatg aaaaacataa aaaagaacat gatgcactga
     1741 cagatcaggt aatggatttt tatatgcggt accaggcagg gaaaaccgtt ttttcacttg
     1801 aattaatgaa ttttttaaaa gattggctga caaatcatat tttaatcagc gacaaaaaat
     1861 acaaaaactt ttttcagtca aaaaatgttg aataatcaag cttttgtcat agcctgaacc
     1921 aatgtataaa ggttctgagc aatgtctttg atagctgatg ctacattgga tacagtattg
     1981 acaatatccg ttagattttg tgcagcatct gacacataaa caatggtctt ggatgcctct
     2041 tcgttggcaa ttcgctgctc actggttgat gtttcaattg tttcgattgc attgtatatc
     2101 tgagtattta gattggcgat agctctaata gatacattaa catcctgaat aagcgtatta
     2161 gtatgcgata tttccttcat tattgttcgt attt
//
LOCUS       DAOSZP010000399         2192 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_38016_length_2192_cov_2.877866, whole genome shotgun sequence.
ACCESSION   DAOSZP010000399 DAOSZP010000000
VERSION     DAOSZP010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2192
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38016_length_2192_cov_2.877866"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="PK348_08550"
     CDS             complement(<1..306)
                     /locus_tag="PK348_08550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66301.1"
                     /translation="MTNQKIDILQKKLQALKPYAIAYSGGNDSTFLAAISKHFSMDFC
                     LIHCISEFTIPGDTQRGRTFAREHDIPYQEITLSVLRTVEIVTNNKDRCYYCKKAVFK
                     "
     gene            complement(378..1928)
                     /locus_tag="PK348_08555"
     CDS             complement(378..1928)
                     /locus_tag="PK348_08555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66302.1"
                     /translation="MRNTVILYLLIYISILLQLEAIALQSISVEYRQDALDYGVKKEH
                     YLRTSATIATDTTEVNAAVVQSHDATLATWSFATQGDYAAIAGGYYFIHNATGLLLGK
                     TSVYNPDPYSIHVQKNDSFVSLCKSGNPQYAMYGVVSSLFDARGASTIKLDAGVSYKE
                     CYISSGDAQNGVYPYSVQGLLSHCKREGQYTEPVQVVTSFVHATLSPVEHVTLQGCYY
                     STHVYYNNAHMLFNANTQDNYAMESFGGYSLFAHYNDSIVSLFSEYAISHVLIKNEGK
                     SGNRYSSAYYCGVIVKEKNYKISSIVQKSDENFFAPFGNTFGGNSPRDMYYYSVSVKP
                     VKKFTCSWIYMDQNNLLPSPYYTEYPHKRINNIRMVYINKYFSVKSDYRYAQFYKDGE
                     ERASRYQQGVVWGITKNLSIHLKYGLYQGDTVAWYTASGIGIKVGNLQNDLGSVYAHT
                     DGEKMYVAMLPLPQTNIISETIAASSFFVVARLRFSNTYCKLSARCVMQLHPQKQTAG
                     EVGAAAWF"
     gene            complement(1913..>2192)
                     /locus_tag="PK348_08560"
     CDS             complement(1913..>2192)
                     /locus_tag="PK348_08560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66303.1"
                     /translation="TYNYYEDETNGIQNHEKQSSIKQNNYIPHEEKNRRIKILFQSMV
                     AAEIPAYIAISLAEEYQKSGEKGVLHSPLFKNLTPSQQKSVKTTCGIQ"
BASE COUNT          669 a          408 c          382 g          733 t
ORIGIN      
        1 tttaaacact gcttttttgc aataataaca gcggtcttta ttattggtaa ctatctccac
       61 agttctcaac actgaaagag taatctcctg ataggggata tcgtgttctc ttgcaaaggt
      121 acgcccacgc tgtgtatcgc ctggtatagt aaattcgctt atacagtgaa taaggcaaaa
      181 atccattgaa aaatgtttgg agatagctgc taaaaaagtt gaatcgttgc caccagaata
      241 ggctattgca taaggcttga gagcctgcaa ctttttttgc aatatgtcta ttttttgatt
      301 tgtcatggga ttacaatacc ataatgttac tgaactatgt ataacgattg tagcacggta
      361 tcaaaaaaac taaaatttta aaaccatgct gccgcaccaa cttcccctgc agtttgtttt
      421 tggggatgta gctgcataac acatcttgct gacagcttac aatacgtatt actgaaacgc
      481 aagcgtgcaa caacaaaaaa tgatgatgcg gcgatagttt ctgaaataat atttgtttgt
      541 ggaagcggaa gcatagctac atacattttt tcgccatcag tatgagcata aactgacccc
      601 aagtcatttt gcagattgcc aacttttatt ccaatacctg atgcagtata ccatgcgacc
      661 gtatccccct gatataaccc atattttaga tggattgata agttttttgt tattccccat
      721 acaaccccct gctggtaacg cgatgccctt tcttccccat ctttataaaa ctgtgcatag
      781 cgataatccg atttaacaga aaaatatttg tttatatata ccatacgtat attattaatt
      841 cgtttatgtg gatattcggt ataatacgga gagggcagaa ggttgttctg gtccatatat
      901 atccatgaac atgtaaattt ttttactggt ttaacactga ccgaataata atacatatct
      961 cgtggtgaat ttcccccaaa tgtattgcca aaaggggcaa aaaagttttc atccgacttt
     1021 tgtacaatac tgctgatttt ataatttttt tctttaacta ttactccaca ataatatgct
     1081 gaactatatc tgtttcctga tttaccttca ttttttatca aaacatgtga aatagcatat
     1141 tccgaaaaaa gtgatacaat actgtcatta tagtgtgcaa acagactgta cccaccaaat
     1201 gattccattg catagttatc ctgtgtattt gcattgaata acatgtgagc attattataa
     1261 tatacatgtg ttgaataata acaaccctgt aacgttacat gctcaacagg gcttagcgtt
     1321 gcatgcacaa aagatgtaac aacctgtaca ggttcagtat actgtccctc ccttttacag
     1381 tgtgatagta agccctggac gctatagggg tataccccat tttgtgcatc accagaagat
     1441 atataacatt ctttatagga aactcctgca tcaagtttaa tggtagatgc acctcttgcg
     1501 tcaaataatg atgaaaccac accatacata gcgtactgtg gattgccact tttacataat
     1561 gatacaaatg aatcattttt ttgaacatga atagaatagg gatcagggtt ataaacagaa
     1621 gtttttccca ataacaaacc tgtggcattg tgaataaaat aatagccacc tgcaattgca
     1681 gcatagtcac cctgagttgc aaatgaccat gtagcaagcg ttgcatcatg tgattgtaca
     1741 actgctgcat ttacttctgt agtatctgtt gctattgttg cgctggtgcg gaggtaatgt
     1801 tcttttttaa cgccataatc aagagcatcc tgcctgtatt caactgatat actctgtaaa
     1861 gctattgctt caagctgcaa tagtatagaa atatatataa gcaaatatag tattactgta
     1921 ttccgcatgt tgttttaaca ctcttttgct gagaaggagt taaattttta aacaaaggtg
     1981 aatgcaatac gcctttttct ccagatttct gatactcttc agctaacgat atggcgatat
     2041 aggcgggaat ttcagcagca accatggatt gaaaaagtat ttttattcgt ctatttttct
     2101 cttcgtgtgg aatgtaatta ttttgtttta ttgaagattg cttttcatgg ttctgtattc
     2161 cgttagtttc atcttcatag taattgtatg ta
//
LOCUS       DAOSZP010000400         2190 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_38045_length_2190_cov_3.292740, whole genome shotgun sequence.
ACCESSION   DAOSZP010000400 DAOSZP010000000
VERSION     DAOSZP010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38045_length_2190_cov_3.292740"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..577
                     /locus_tag="PK348_08565"
     CDS             <1..577
                     /locus_tag="PK348_08565"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HXK66304.1"
                     /translation="KLYDNLVYVTWSTGIGAGAYVDGKLIRGKNGNAPHFGHIYMLED
                     GPQCGCGNYGDLEAIASGTAIARDYGVHSAKEVFDNYRRGDTKAIHIVHRAARNFARG
                     LASLNALFDTEYIVLGGSVMNDSDILLPLMREEFYRSFPVLSKSVIIEKSVLFEYIAD
                     ISALSLVMPSSWIESWRTYMPWKNAPELIKL"
     gene            complement(596..922)
                     /locus_tag="PK348_08570"
     CDS             complement(596..922)
                     /locus_tag="PK348_08570"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HXK66305.1"
                     /translation="MNIKHEIKDRVLIISIEGELISPQALDTDKHIIALIEKEKKERV
                     IIDLTKVDFIDSTGLGILVTIRLYCKKNKILFATVGVNEKIKRTFTVTEMTSFLNIHD
                     TIDKLL"
     gene            complement(1016..1651)
                     /locus_tag="PK348_08575"
     CDS             complement(1016..1651)
                     /locus_tag="PK348_08575"
                     /inference="COORDINATES: protein motif:HMM:TIGR01470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HXK66306.1"
                     /translation="MLYPIMYKIKGKRVVVIGGGQVAFRKVKDLANCGATVVVIAPDI
                     HPELEEYATANPAITIIKREYQAGDCAGSALVFSATNNPRVNRTVYEEALALNIPINA
                     VDDPDNSSFYVPSFFTRGPLLVAVSTGGASPAMAAKVRRLIEQSIPQDIEITLDTLNE
                     ARIILKEHYYHLTSHQRSELLKAIVTDERLLEQAVEAFKNNMLQEFLKSLL"
     gene            complement(1660..>2190)
                     /locus_tag="PK348_08580"
     CDS             complement(1660..>2190)
                     /locus_tag="PK348_08580"
                     /inference="COORDINATES: protein motif:HMM:TIGR04085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HXK66307.1"
                     /translation="RTNDFFRRGLDINILTVDNHVDGVYVYRKLLQKDEQKANQVKDL
                     LTWNGGGAYSTGVGIANIDFVGNVHPDQFWQDYTFGNVLERNFADIWMDETDPLMKGL
                     KHKAEYIKGRCRVCQYKAMCNGSMRVRAYRVFGDPWAPDPQCYLTDEEIGLTDKTIAE
                     LKTNGEYFEMPAELKK"
BASE COUNT          613 a          452 c          444 g          681 t
ORIGIN      
        1 caaattatat gataatcttg tgtatgttac ctggagtaca ggcataggtg ctggtgcata
       61 tgttgacggc aagctcattc gtggtaaaaa cggtaatgct ccgcactttg gtcatatata
      121 catgcttgag gatggtccgc aatgtggttg tggaaactat ggggatttag aggcgatagc
      181 atctggcaca gcaatagccc gtgattatgg agtccactct gccaaggaag tctttgacaa
      241 ctatcgtaga ggcgatacaa aggctataca catagtacac cgggctgcac gaaattttgc
      301 gcggggactg gcttcactca atgcattatt tgatacggaa tacattgtat taggtggtag
      361 cgtcatgaat gacagcgata ttctgctgcc acttatgcgg gaagagttct atagatcatt
      421 ccctgtgtta tcaaaaagcg ttattataga aaaaagtgta ttgtttgaat atattgctga
      481 tatatctgca ctgtcgcttg ttatgccttc ttcatggatt gaatcgtgga gaacatatat
      541 gccgtggaag aatgcgcctg agctgatcaa actataacaa tacagttaat gacaatcaaa
      601 gaagtttatc tattgtatcg tgaatattta aaaatgatgt catttcggta acggtgaagg
      661 ttcgtttgat cttttcatta actccaaccg ttgcaaacag aatcttgttc tttttgcaat
      721 ataaccgtat tgtaaccagt attcccaatc ctgttgaatc aataaaatca acttttgtga
      781 gatcaataat aacgcgctct tttttttctt tttcaattag agctatgata tgtttgtcag
      841 tatccaaagc ctgaggggat attaattctc cttcaatgga tataatcagt actctgtctt
      901 ttatttcgtg tttgatattc atcctgataa taacctccgg cacacaattt ctattgtaaa
      961 cccttccata aagatgaata tgaaaaaact tttaaagaaa atcaagcatt atttattata
     1021 gcagtgattt caaaaactct tgcagcatat tatttttaaa agcttcgaca gcctgttcaa
     1081 gaagcctttc atcggtaact atcgctttta aaagttcact tcgttggtgg gatgtaagat
     1141 ggtagtagtg ttcttttaat atgatgcgtg cttcattcaa cgtatcaagt gttatctcga
     1201 tatcctgtgg gatactttgc tcaatgagcc ttcgcacttt tgctgccata gcaggtgaag
     1261 caccacctgt ggaaacagca acaagaaggg ggcctcgtgt aaaaaacgat ggcacgtaaa
     1321 acgatgaatt atcgggatca tctaccgcat ttatgggaat atttaacgcc aatgcttctt
     1381 catagactgt gcgatttacc ctgggattat ttgttgctga aaaaacaagc gctgaaccag
     1441 cacaatcgcc tgcctggtat tcgcgtttaa tgatggtaat ggctggattg gctgtagcat
     1501 attcctccag ctcaggatga atgtcaggag ctatcaccac aaccgtagca ccgcagtttg
     1561 ccagatcttt gactttacga aatgcaacct ggccgccacc aatgaccaca acgcgcttcc
     1621 cctttatttt atacattatt ggataaagca tagtgcatcc tactttttca attcagctgg
     1681 catttcaaaa tattctccat tggtttttaa ctctgcaatt gttttatcag ttaatccaat
     1741 ctcttcgtcg gttaggtagc actgaggatc gggagcccat gggtcaccaa acacacggta
     1801 tgctcgcaca cgcattgagc cgttacacat ggctttgtac tggcacaccc tgcagcgtcc
     1861 ttttatatac tcagctttat gcttcaatcc tttcataaga gggtctgttt catccatcca
     1921 gatatcggca aagttgcgct caagcacatt gccaaaggta taatcctgcc agaactggtc
     1981 aggatggaca ttgccaacaa aatcaatatt tgcaatgcca actcccgtgg aataggcacc
     2041 accgccattc caggttaata gatcctttac ctggttggct ttttgttcat ctttctgtag
     2101 caacttgcgg tacacgtaca ctccatcaac atgattatcc acggtaagta tgttgatgtc
     2161 aagcccgcgt ctgaagaaat cattagtgcg
//
LOCUS       DAOSZP010000401         2188 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_38091_length_2188_cov_2.919362, whole genome shotgun sequence.
ACCESSION   DAOSZP010000401 DAOSZP010000000
VERSION     DAOSZP010000401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38091_length_2188_cov_2.919362"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1012
                     /locus_tag="PK348_08585"
     CDS             <1..1012
                     /locus_tag="PK348_08585"
                     /inference="COORDINATES: protein motif:HMM:TIGR02494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HXK66308.1"
                     /translation="AIDSTGIVLEIQRMSTEDGPGIRTTVFMKGCPLKCVWCHNPESI
                     NPKPQVQWIGARCIGCKTCVQACPNGALTFDDGIHINRTVCKGCGRCTEVCPSTAMEL
                     LGKQWCADALVQELLNESAYFGTSGGGVTISGGESTFQAHFVKKVLVELKHHGIHTAI
                     DTCGICNQHTLDMLLPHVDLVLYDLKEMDPLKHQQFTGKSNYRVLNNIKYILSFMKEH
                     LFPREVWIRTPLIPGKTARDENIVAIAGEINNLLSINPKAITRWDLCAFNNLCKDKYT
                     RLGLEWELAHTPLLTKEELSHFEALAKQHCNNPAIVKTSGSTRIEDDEAPVTKPKKKL
                     GC"
     gene            1035..1880
                     /locus_tag="PK348_08590"
     CDS             1035..1880
                     /locus_tag="PK348_08590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66309.1"
                     /translation="MKTRKSFTIDEIAALKPNEKIALVATINNEGLPHVTLLTSLLAL
                     DDTHMALGEFSKGVSKEYIKYNNNIGFCILTMNMDMWRGKARYTHSAKEGPEYEMFNE
                     FPMFRYNTYFGINTVHYFDLLEANEKERLPLAAIAKAVIATLCSKWAAKQKKDTRPLD
                     TPGFELFNRLDALKFAAWIDSDGFPKIVPVLQAKASDSSTIVFSPLAFREELLQLKEG
                     TPVAVYALTVKMENILTRGIFKGYRRYAGITLGMCDINWVYNSMPPVHGQIYPRVPLT
                     PVKDF"
     gene            2018..>2188
                     /locus_tag="PK348_08595"
     CDS             2018..>2188
                     /locus_tag="PK348_08595"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HXK66310.1"
                     /translation="MVSKGTVTKQTIINRIIEHIHLNGFSETSLSDIIAITGVKKGNL
                     YFHFKNKEELILE"
BASE COUNT          702 a          402 c          473 g          611 t
ORIGIN      
        1 ggctattgat tccactggca tagtacttga aatacagcgc atgtctactg aggatggccc
       61 gggtatacgc accacggtat ttatgaaagg ctgcccgctc aagtgtgtgt ggtgccacaa
      121 tcctgaaagc ataaatccaa aaccgcaggt gcagtggatt ggtgcccgct gcattggatg
      181 taaaacgtgt gtgcaggcat gtcccaatgg cgcgctgacg tttgatgatg gcatacatat
      241 taatcgcact gtgtgtaaag gatgtggtag gtgtactgaa gtgtgtcctt caactgccat
      301 ggagcttttg ggcaaacagt ggtgtgctga tgcactggta caggagcttt taaacgagag
      361 tgcatatttt ggcacctcag gaggtggtgt aaccatttca ggtggcgaaa gtaccttcca
      421 ggctcatttt gtaaaaaagg tgctggtgga actcaaacat catggcattc acaccgcaat
      481 tgacacctgt ggtatatgta accagcatac gcttgatatg ctgctgcccc atgttgatct
      541 tgtgctctat gatcttaaag aaatggatcc tctaaaacac caacagttta ccggtaagtc
      601 aaattataga gtgcttaata atatcaaata tatactttct tttatgaaag aacatctgtt
      661 cccacgtgag gtgtggatac gtacgccact tatcccgggt aaaactgcac gagatgaaaa
      721 tatcgtggcg atagctggtg aaataaacaa tctgctatct attaatccaa aagctattac
      781 tcgctgggat ttgtgcgcat ttaataatct atgcaaggac aagtatacca ggctggggct
      841 ggaatgggaa ctggcacata cgccactttt aactaaagag gaactaagcc attttgaagc
      901 actggcaaag cagcattgca ataatccggc aattgtgaaa acaagcggtt caacccgtat
      961 agaagatgat gaagcgccgg taacaaaacc aaagaagaaa ttaggatgct aatagtatgt
     1021 atggaggaat ataaatgaaa acacgaaaat cattcaccat tgatgaaatt gcagcattaa
     1081 aaccaaatga aaaaatagca cttgttgcca caattaataa cgaaggatta ccccatgtaa
     1141 cattgctcac gtcactactt gcactcgatg acacacatat ggcactgggt gaattttcaa
     1201 aaggtgtaag caaagaatat ataaagtaca acaataacat aggtttttgc atacttacca
     1261 tgaatatgga tatgtggcgt gggaaagcac gttatacgca cagtgccaaa gaaggtcctg
     1321 aatatgagat gttcaatgaa tttcccatgt tcagatacaa tacatacttt ggcattaata
     1381 ctgtgcatta ttttgattta cttgaggcaa atgaaaagga acgcttgcct ttggcagcta
     1441 tcgcaaaagc tgttattgca acgctatgtt caaaatgggc tgcaaaacag aaaaaagaca
     1501 ccaggcccct tgatacacca ggctttgaac tttttaacag gctggatgcg cttaaatttg
     1561 ccgcctggat tgacagtgat ggttttccaa aaatagtgcc ggtactgcag gcaaaggcca
     1621 gtgattcctc taccatagtg ttctcgccgt tagcttttcg tgaagaattg ttacaattaa
     1681 aagaaggaac accggttgca gtgtatgctc taacagtaaa gatggaaaat atactgacac
     1741 gggggatttt caaaggctac aggcggtatg caggtattac gcttggtatg tgtgatatca
     1801 actgggtata taattctatg ccaccagtcc atggtcagat atatccccgg gtaccgctaa
     1861 caccggtaaa ggatttttaa attgtccttg tattgatgtt ttgttagtta atgtttttta
     1921 ttaattgttt gtttttaaaa taaaaaacat ataataatca ttgacaaatt tgttttatca
     1981 aaataatgta ccaactggtc agtacaggat ggtatatatg gtatcaaaag gaacagtaac
     2041 aaaacagaca atcattaacc gcatcattga gcatatacac ctcaatggtt tttctgaaac
     2101 aagtctcagt gatattattg ccattactgg cgttaaaaaa ggtaatctat attttcattt
     2161 taaaaataaa gaagaactaa ttttagaa
//
LOCUS       DAOSZP010000402         2183 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_38208_length_2183_cov_3.460526, whole genome shotgun sequence.
ACCESSION   DAOSZP010000402 DAOSZP010000000
VERSION     DAOSZP010000402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38208_length_2183_cov_3.460526"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="PK348_08600"
     CDS             <1..222
                     /locus_tag="PK348_08600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66311.1"
                     /translation="HIPVLKGDSAIYSMVVHVADTFAKVMKVGFSGNYVIDAIDEAIW
                     QTLGISEEIVFEALAPLNDEIQKAKLFLQ"
     gene            219..1097
                     /locus_tag="PK348_08605"
     CDS             219..1097
                     /locus_tag="PK348_08605"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXK66312.1"
                     /translation="MSKLTVTFWGVRGSIPVPGPDTMKYGGNTACLDLRSDAGDWIVF
                     DAGTGLRVLGESLDLSKNYTVNLCISHPHWDHINGFPFFPVIYIPGNTVNIYGPGTFE
                     KSLEEIIRGQMQYSYFPVRTDELRASIHFYKIKNNQFEVGNFKVYTHLLNHPVTCYGY
                     KVLYKDKIFVYLGDNEPYYNVYKDNDPEVIEFAKQMNEQLIEFVKGADVLVSDAQYIP
                     AEYSKKTGWGHSTTHHVINLALKAKVKTLFLFHHEPLRKDREMDAIVTHYRNVLKKKE
                     LSLELFAAAERQSFTF"
     gene            complement(1098..1895)
                     /locus_tag="PK348_08610"
     CDS             complement(1098..1895)
                     /locus_tag="PK348_08610"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HXK66313.1"
                     /translation="MINIVAACIAVSFIAKPFTMLLRSIQSLVNDRYTSSNPCSKEHL
                     IMLTQELEQFKARSDSLLREKDTIVNELIESKKLLEKYSNNLEEMVEQRTEILKWLSI
                     TDPLTGLYNRRYFIEQIELEFKRSKRYNRHLSLLMLDIDHFKSVNDNYGHQVGDIVLR
                     KISSIIISQLRDSDLAFRYGGEEFMVILPETKADDAINVAKRMKQEIMETEHIYRNLN
                     FKVTASIGIVSIKDMLGKFETVDDIIKKVDDNLYKAKNSGRNTIIYE"
BASE COUNT          680 a          379 c          421 g          703 t
ORIGIN      
        1 catataccgg ttttaaaagg tgattcagcc atctattcca tggttgttca tgtagcagat
       61 acgtttgcca aggtcatgaa agttggattc agtgggaatt atgttattga tgctattgat
      121 gaagctatat ggcaaacgct tgggatatcc gaggagatag tttttgaggc attagctcct
      181 ctgaatgatg aaatacaaaa ggctaaatta tttttgcaat gagtaaactg accgtaacat
      241 tctggggtgt gcgtggttct atacctgttc ccggccctga tactatgaaa tatggaggga
      301 atactgcctg cctggattta cgatcagatg caggtgactg gatagtattt gatgcaggga
      361 cagggcttag ggtactgggc gaatcactgg atctgtcaaa aaactatact gtcaatttat
      421 gcatttctca tcctcactgg gaccacatta atggttttcc ttttttccct gttatctaca
      481 taccaggaaa tacggttaat atctatggtc ctggaacatt tgaaaagagc ctggaggaaa
      541 ttatacgcgg gcagatgcag tacagttatt tcccggtccg caccgatgaa ttacgcgcca
      601 gtattcattt ttataaaata aaaaataacc agtttgaagt cggtaacttt aaggtttaca
      661 cgcaccttct caatcatccg gtaacctgtt atggatataa agtactatat aaagataaga
      721 tttttgttta tctgggcgac aatgagccat attataatgt gtataaggat aatgacccgg
      781 aagtaattga atttgcaaaa cagatgaatg agcagcttat tgaatttgta aaaggtgcag
      841 atgtattagt atcagatgca cagtatattc ctgctgagta ttcaaagaaa accggttggg
      901 gccacagtac aacgcatcat gttattaatc tggcacttaa ggcaaaagtt aaaacattat
      961 ttttatttca tcatgagcct ttacgcaaag acagggaaat ggatgcaata gttactcatt
     1021 ataggaatgt gctgaagaaa aaagagctat cgcttgaact gtttgcagca gctgaaaggc
     1081 agtcctttac attttaatca ttcatatata atggtattcc tgccggaatt ttttgcctta
     1141 tagagattat catccacctt tttgataata tcatcaaccg tttcaaattt gcccagcata
     1201 tccttgattg atacaatgcc tatgcttgct gttactttaa aattaagatt acggtagatg
     1261 tgttccgttt ccattatttc ctgtttcatc cgttttgcaa cattgatagc atcatctgct
     1321 tttgtctcag gcagtattac cataaattct tcaccaccat accgaaaggc aaggtctgaa
     1381 tcacgaagct ggcttattat tattgacgat atcttccgta aaacaatatc accaacctga
     1441 tggccgtaat tatcgtttac tgatttaaag tgatcaatat caagcatcaa aagcgataag
     1501 tggcggttgt aacgtttgga acgtttaaat tcaagttcaa tctgttctat gaaatatctt
     1561 ctattgtaaa gccctgttag cgggtcagta attgaaagcc actttaatat ttcggtgcgc
     1621 tgttcaacca tttcttcaag gttattggaa tatttttcta atagcttttt ggattcaatc
     1681 agttcattaa ctatggtgtc cttttcacgc aacaatgaat cggaccttgc tttaaattgt
     1741 tcaagttctt gtgtcagcat gatcagatgt tcttttgagc aagggttgga agatgtgtac
     1801 ctgtcattaa ccagtgactg gattgaacgc aaaagcattg taaagggttt tgcaataaat
     1861 gaaaccgcaa tacatgcggc aacaatattt atcacaaaga agcttatcac agtgaaaatg
     1921 gtaaaactaa tagctttatt tataaaaaga aataggcaga tagttcctga tacaaaggta
     1981 ttatacagaa aaaatttaat aaaaagcgaa attgtaattt tatggtgcat actttataaa
     2041 gccttatcag tcataaaggc cattacaaat tccttcaata agagctataa cattttttcc
     2101 taaatcaggg atatagtaac caccttctaa aatggcaaat atatggcctt tgcagtaatg
     2161 tcttgcatga ttaccagcta tct
//
LOCUS       DAOSZP010000403         2179 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_38295_length_2179_cov_2.882768, whole genome shotgun sequence.
ACCESSION   DAOSZP010000403 DAOSZP010000000
VERSION     DAOSZP010000403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2179
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38295_length_2179_cov_2.882768"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(8..1315)
                     /locus_tag="PK348_08615"
     CDS             complement(8..1315)
                     /locus_tag="PK348_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HXK66314.1"
                     /translation="MMNSDCVANIILDSIADGVFTIDMDFRITSLNNAACKILGLESK
                     DEAIGKLCFEIFHANICEHSCALRETMQTGKNIINKTVYIVNKSGEKVTISISTALLK
                     DDQGNIIGGVETFRDISDIETLRKKIESTYSFEDIISKNKEMQQIFSILPDVALSDSS
                     VLIEGPSGSGKELIARAIHSLSRRKEKPFVVVNCGALPENLLESELFGYKTGAFTDAK
                     KDKPGKIAMAEGGTLFLDEIGELPLSMQVKLLRFLQFKEYEPLGDTKPKKANVRIIAA
                     TNKNLREEVAAGNFRDDLYYRLNVIGLRLPSLSQRKEDIPLLVEHFIKKYNTLKGKSI
                     EGVSDDAMNILLNYDYPGNIRELENIIEHAFVLCHEAYIKLEHLPAYVYGITPVSTLS
                     LEEMEKTMIKQALARYSNKKDAAAALGIDTSTLWRKIKKYNLE"
     gene            complement(1312..2151)
                     /locus_tag="PK348_08620"
     CDS             complement(1312..2151)
                     /locus_tag="PK348_08620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66315.1"
                     /translation="MKKFYIIAIISCILTIGCFDFDIVHYVEPKKDRSLFIKFRVTSI
                     YFKDQTDKKTFNNDVIKGNLPDEKDTQVTYQWIENDLTAGVEITTTAKPHSTISATDK
                     FPVIPYRDKFGQYIFIYHNYQELGSSQNLFDSQKIAEGLLAASKYRLAFGNTVPKQAL
                     IIVYREQPEKFNLSIYQLGNIYCIDIPMNLIIMNESAVIVSCASTINDSEISNYLTKL
                     LEKRKEEEKKHEELKKQENNENDATNNYNDSNDEDNSDTQFDNNHNNDEHENIDNDTD
                     NVE"
BASE COUNT          625 a          396 c          352 g          806 t
ORIGIN      
        1 gcaatattca ttcaaggtta tattttttta ttttccgcca taatgttgag gtatcaatac
       61 caagtgcggc agctgcatct ttcttgttag aataccgtgc taaagcctgt tttatcatgg
      121 ttttttccat ctcttcaagc gagagtgttg aaacaggtgt tatgccatat acataggcag
      181 gcaggtgttc aagctttatg tatgcctcat gacataagac aaatgcatgt tcaatgatgt
      241 tttcaagttc acggatattg cctggatagt cgtaattaag caagatattc attgcgtcat
      301 cggacactcc ttcaatgctt ttccctttta aggtattgta ttttttaata aagtgttcca
      361 ccagtaatgg tatatcttct tttcgctgtg ataatgatgg taagcgtaat cctatgacat
      421 tcaggcgata gtataaatcg tcgcgaaaat taccagccgc aacttcttct cgcaaattct
      481 tattggtggc agcaataatg cgtacattag ctttttttgg ttttgtgtcg ccaagaggtt
      541 catattcttt gaattgcaaa aatcgaagta atttcacctg cattgataat ggcagttcgc
      601 caatctcatc cagaaacagt gtgccacctt cagccatggc tattttgcca ggtttatctt
      661 tttttgcatc tgtgaacgca ccagttttgt atccaaacaa ttctgattca agcaaatttt
      721 caggtaatgc tccgcaattt accacaacaa atggcttctc ttttcgcctg cttaaagaat
      781 gaatggcacg tgcaatgagt tctttcccgc ttccactggg tccttcaatt aacacagagc
      841 tatcgcttaa ggctacatca ggcagtatgg aaaaaatctg ctgcatctct ttatttttac
      901 ttatgatgtc ttcaaaagag taggtggatt caattttctt tctcaaagtt tcaatatcgc
      961 tgatatcacg aaatgtttcc acgccaccaa tgatatttcc ctgatcatct ttgagtaatg
     1021 cggtacttat gcttatggtt actttttcac ctgatttgtt aactatatat actgttttat
     1081 taataatatt ttttcctgtc tgcatggttt cacgaagcgc acaggaatgc tcacaaatat
     1141 ttgcatgaaa gatttcaaag caaagcttac caatagcttc gtctttactt tcaagaccta
     1201 aaattttaca ggcagcatta ttcaatgacg tgattctgaa atccatatca atggtaaaca
     1261 caccatcagc aatactgtct aaaataatgt ttgctacaca atcagagttc atcattctac
     1321 attgtctgtg tcattgtcaa tgttttcatg ttcatcatta ttatggttat tatcaaactg
     1381 tgtgtcacta ttgtcttcat cattactatc attataattg tttgtagcat cattttcatt
     1441 attttcttgt ttctttaatt cttcatgttt tttttcttct tctttccgct tttcaagtaa
     1501 ttttgtaaga tagttactga tttcagaatc gttgattgta ctggcacatg atacaatcac
     1561 agcagattca ttcataatga tgagattcat tggtatatca atacaataaa tattcccaag
     1621 ctgatatatc gaaaggttaa atttttcagg ttgttcacgg taaactatga tcaatgcctg
     1681 tttaggtact gtattaccaa atgccagtct atatttggat gctgccagta atccttcagc
     1741 tattttttgt gaatcaaaaa gattctgtga agaacctaat tcctgatagt tgtgatagat
     1801 aaaaatatat tgtccaaatt tatcccggta gggtataact ggaaatttat ccgttgcaga
     1861 aatagttgaa tgtggtttag ctgtagtagt aatctctaca cctgcagtta ggtcattctc
     1921 tatccattga taggtaacct gtgtatcttt ttcatctggt aagtttccct ttataacatc
     1981 attattaaac gttttcttat ctgtttgatc tttgaaatag attgaagtta ctctaaattt
     2041 tataaataat gacctgtctt tttttggttc aacgtaatga acaatatcaa aatcaaaaca
     2101 gccgatagtc aatatacatg aaataattgc aatgatgtaa aattttttca taaaaaattc
     2161 tccaaatttt taagaatgt
//
LOCUS       DAOSZP010000404         2172 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_38444_length_2172_cov_3.975909, whole genome shotgun sequence.
ACCESSION   DAOSZP010000404 DAOSZP010000000
VERSION     DAOSZP010000404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38444_length_2172_cov_3.975909"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..323
                     /locus_tag="PK348_08625"
     CDS             <1..323
                     /locus_tag="PK348_08625"
                     /inference="COORDINATES: protein motif:HMM:NF014460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXK66316.1"
                     /translation="TPVLVVVTDVVGSLGGAFIAYFNLGITIDKYVENVFNYLGVMDF
                     LTGLFKSVFFGIEIAIIACYQGFNTSGGAEGVGRATIRAVVASSMTILISDYFLTYLL
                     QFIE"
     gene            340..984
                     /locus_tag="PK348_08630"
     CDS             340..984
                     /locus_tag="PK348_08630"
                     /inference="COORDINATES: protein motif:HMM:NF013315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HXK66317.1"
                     /translation="MKSLKKRKEEFIKFLQKNKIPKKVLDAFVAVNQQDFFDEIYEDR
                     LYGQLPIPIGRGETSDEPLALARMIQYCGIKKDFHVLEIGTGSGYSTAVLSQLAKSVT
                     TIEIDEELAVQAKQRFKKTGLSNIRIFHGDGTDIDESLGFFDAIIVLAGCKKRPLLLL
                     HVAKPDSMIVFPMGPAHQQQIALMLNHRDDNVEGLYPVVFKEFCSFMPIIGIYG"
     gene            complement(1030..1374)
                     /locus_tag="PK348_08635"
     CDS             complement(1030..1374)
                     /locus_tag="PK348_08635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66318.1"
                     /translation="MIETILYILSFGAGIAVFLYLLSLYHHIKERPKPDNKQPAQKVT
                     QDKIQFKSQPVNEPVHLKERTCPVCGSKLSKYEALYASKIVKDSTKILIYGCRYCYKP
                     QEDPDKKKKSDI"
     gene            complement(1371..2099)
                     /locus_tag="PK348_08640"
     CDS             complement(1371..2099)
                     /locus_tag="PK348_08640"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXK66319.1"
                     /translation="MKPYHYLAPYYDYMLKHVDYATWYHYLRMIMCRYINNPRTVLEL
                     GCGTGKFGPKFSMDDYEIYGMDKSLEMLKIAKARAYKHYHVFCGDITQFALSKTIDFI
                     FSVHDTFNYILDYDDFVSALHCVHSCMNNDSIFLFDLTTEYNIKKNFHNKLLTFQVKG
                     TDIQWYNEYDKHSKILYSKLTFTRHTNTLVEEHLQRLYTIDEVLPLIEQCGLTVVDVV
                     GDYTLKPPHEETIMVNFITKRAQR"
BASE COUNT          637 a          358 c          433 g          744 t
ORIGIN      
        1 tgacgccagt acttgttgtt gttacagatg ttgttggttc attaggtggg gcattcatag
       61 catactttaa tttagggata actatcgaca aatatgttga aaatgttttt aactatttag
      121 gtgtcatgga ctttttgaca gggcttttta aatctgtttt ttttggcatt gaaattgcca
      181 ttattgcctg ctatcagggg tttaatacat ctggtggggc tgagggtgtt ggcagggcta
      241 caatacgggc tgttgttgcg tcatccatga ccatacttat ttcagactat tttcttactt
      301 atcttttgca atttattgag tagtggaagt gcagggctta tgaaatcgct gaaaaaaaga
      361 aaagaggaat ttatcaagtt tttgcaaaaa aataaaattc caaaaaaggt gctggatgca
      421 tttgtagcag tcaatcagca ggattttttt gatgaaattt atgaagacag gttatatggg
      481 caactgccaa ttccaatagg cagaggcgaa accagcgatg aaccgcttgc acttgcccgc
      541 atgatacagt attgcggcat taagaaagat ttccatgttc ttgagattgg tactggttca
      601 ggctattcaa ctgcagtgtt gtcacagctt gcaaaatcag taaccaccat tgaaatagat
      661 gaagaacttg ctgttcaggc aaaacaacgc ttcaaaaaaa caggattgtc aaatattcgt
      721 attttccatg gcgatggtac agatattgat gaatcattag gtttttttga tgcgataata
      781 gtgctggcag gttgtaaaaa acggccattg ctattactcc atgttgccaa acctgatagc
      841 atgatcgtat tccccatggg tcctgcccat cagcagcaga tagcactgat gcttaatcac
      901 cgggatgata atgttgaggg gttgtatcca gttgttttca aggaattctg cagtttcatg
      961 cccatcattg gaatatatgg ctaagtacga taatgactat ataataatgt gctttgaaaa
     1021 cagtaatgct tatatatctg atttcttttt tttatctgga tcttcctgtg gcttataaca
     1081 atatcgacat ccatatatta atatctttgt gctatccttt acaatctttg aggcatataa
     1141 tgcttcatac ttgctgagtt ttgatccgca tactgggcat gtcctttctt ttaaatgtac
     1201 gggttcatta acgggttgtg atttgaattg tattttgtcc tgcgttactt tttgtgcagg
     1261 ttgcttgttg tcaggttttg gacgttcttt aatatgatga tacagggaaa gcagatataa
     1321 aaacactgca atgcctgcac caaatgaaag aatataaaga atagtttcaa tcatcgttgt
     1381 gctcgttttg taataaaatt aaccataatt gtttcttcat ggggtggctt aagcgtataa
     1441 tccccaacaa catcaactac tgtaagaccg cattgttcaa taagaggtag tacttcatca
     1501 attgtatata atcgctgaag atgttcttct accagtgtat tggtatgcct ggtaaatgtc
     1561 aactttgagt ataatatttt tgaatgttta tcatattcat tgtaccactg aatatcggtg
     1621 cctttaactt gaaatgttaa tagcttattg tgaaaattct ttttaatatt atactctgtg
     1681 gtaagatcaa ataaaaagat actgtcattg ttcatacagc tatgcacaca gtgtaatgcg
     1741 ctgacaaaat catcataatc caggatatag ttaaatgtat cgtgaacaga aaaaataaaa
     1801 tcaattgttt ttgacagagc aaactgtgta atgtcgccgc aaaatacgtg atagtgttta
     1861 tatgctcgcg cttttgctat ttttaacatt tcaagtgatt tatccatgcc atatatttca
     1921 taatcatcca tggagaactt tggaccaaat ttgccagtac cacatcccaa ctccagcact
     1981 gttcgtggat tattaatata cctgcacatt atcattcgca gataatgata ccaggtggca
     2041 taatcaacat gtttcagcat atagtcataa tagggagcaa ggtaatggta tggtttcatt
     2101 gtatttttcg gttcaatcga ttaattctgt gggtgaaacg ctcagtgtag tgttcaatga
     2161 gcttctggtt at
//
LOCUS       DAOSZP010000405         2171 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_38469_length_2171_cov_3.662098, whole genome shotgun sequence.
ACCESSION   DAOSZP010000405 DAOSZP010000000
VERSION     DAOSZP010000405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2171
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38469_length_2171_cov_3.662098"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /locus_tag="PK348_08645"
     CDS             <1..415
                     /locus_tag="PK348_08645"
                     /inference="COORDINATES: protein motif:HMM:NF014658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66320.1"
                     /translation="LHDVDAKLVIGNEAMMHETEPLPYIYDLGDLWLQKTGYPVVFAV
                     FAVRNDAIDYAETINAVCASYRMSLNELAKNPHDVIEKARQRYPDIAYDIAHYYTLLK
                     FEFTDELKKALLYYYSLASELQLLPQVPSLQLYGR"
     gene            425..1495
                     /locus_tag="PK348_08650"
     CDS             425..1495
                     /locus_tag="PK348_08650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CofH family radical SAM protein"
                     /protein_id="HXK66321.1"
                     /translation="MKSIDNIYHAVADRRRIDPHDAVSLYQYDIATLGVLANMRREQI
                     VPGNEVGFVIDRIINYSNRCVAMCNFCAYHARAGKIPPYDLTIDDILQKINELVAIGG
                     TQVMLQGGLHPEYTLQHYINMVKTIKKHYPGIYLHSFSPSEVVHIAKQSNCSIDDVIG
                     SLQTAGLDSIPGASDLLVDEVRMRVSPRKITVQQWCDVVRSIKKHGMHTTATMTYGMG
                     ETLEQRIEHLEVVRRMQDETGIIMGFIPWSFSPKGTYMDDIIPATGLDYLKTVAIARI
                     FLDNVLYLHAGWLTEGMALAQIALAMGANDMGGILMEEKVVKATGVTTTTNIEQICDI
                     IINAGKVPLQRDSKYRPIRRYV"
     gene            1492..>2171
                     /locus_tag="PK348_08655"
     CDS             1492..>2171
                     /locus_tag="PK348_08655"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone/menaquinone biosynthesis
                     methyltransferase"
                     /protein_id="HXK66322.1"
                     /translation="MRYRVDNDTPHERKAKIRSMFDSIVDGYDRANRILSLGVDRSWR
                     KTVVEYCECKPSDVVLDICCGTGDLSALIRGTGAHVVSLDFSENMIQKGREKGNIDDL
                     ATVADASKLPFKDNIFTKVCISFGIRNIPDIDNFLEEVYRVLRPGGTFIILELTRPRY
                     NIFRLLYFFYLYTILPVLGGIIAGSFNAYRYLSKTIHTFIDPVTLTAMQEKHGFIDAV
                     THEQLAQA"
BASE COUNT          644 a          414 c          487 g          626 t
ORIGIN      
        1 gctgcatgat gttgatgcaa agcttgtcat tggtaatgaa gcaatgatgc atgaaacaga
       61 gccactgccg tatatctatg acctgggtga tctgtggctt cagaaaacag gttaccctgt
      121 ggtctttgcc gtgtttgcag tgcgcaacga tgcaatagac tatgctgaaa ccattaacgc
      181 ggtgtgtgcg tcataccgca tgtcattgaa tgagcttgca aaaaatcccc acgatgttat
      241 tgaaaaggca cggcagcgtt accctgacat agcctatgat attgctcatt actacacatt
      301 gcttaagttt gaatttacag atgagcttaa aaaggctttg ctgtattact attcactggc
      361 ttcggagctt caattgttgc cacaggttcc atcattacaa ttatatgggc gatagttact
      421 ggatatgaaa tcaattgaca acatatacca tgctgtagct gaccgcagaa ggatagatcc
      481 gcatgacgcc gtttccctgt atcaatacga tatagctacg ttaggtgttt tagcaaacat
      541 gcgtcgcgag cagatagttc ctggaaatga agttggcttt gtcattgacc gcatcataaa
      601 ttattccaac aggtgtgtgg caatgtgcaa tttttgcgca tatcatgccc gtgcaggaaa
      661 aataccgccg tatgatttaa ccatagatga cattttacaa aagattaatg aacttgttgc
      721 cataggtggc acgcaggtca tgttgcaggg tggattgcat cctgaatata cgttacagca
      781 ctatattaac atggtaaaaa caataaagaa gcattatccc ggtatatacc tgcattcatt
      841 ttccccatcc gaagtggttc atatagcaaa acaaagtaat tgcagcattg atgatgttat
      901 tggcagtcta caaactgcag gtcttgattc aataccaggt gcttctgact tgcttgtgga
      961 tgaagtacgc atgagagtaa gcccgcgtaa aattacagta cagcagtggt gtgatgtagt
     1021 tcgttcaata aagaaacatg gcatgcacac cacagcaacc atgacctacg gcatgggcga
     1081 aacattagag cagcgaatag agcatttaga agtagttcgc cgcatgcagg atgaaacagg
     1141 tattatcatg ggctttattc cctggtcatt ttcgccaaag ggtacgtaca tggatgatat
     1201 aatccctgca actggcctgg attatctaaa aactgtcgcc attgcacgaa tttttctgga
     1261 caatgtgcta tatcttcatg caggatggct taccgagggg atggcattag cacagattgc
     1321 acttgcaatg ggtgcaaacg atatgggtgg gattttgatg gaagagaagg tggtaaaagc
     1381 aacaggcgta accacaacca ccaatataga acaaatatgt gatatcatta ttaatgcggg
     1441 aaaagttccc ctgcagcgtg attctaagta caggcctatc aggagatatg tatgagatac
     1501 agggttgata atgatactcc tcatgagcgc aaagcaaaaa tacggtcaat gtttgattcc
     1561 attgttgatg gctatgacag agcaaaccga attttatcgt taggcgtaga caggtcatgg
     1621 cgtaaaacag ttgtagagta ctgtgaatgc aaaccgtctg atgtggtact tgatatatgc
     1681 tgtggtactg gtgatctgtc tgcattaatc cgtggtacag gtgcacatgt tgtgtcgctt
     1741 gatttttcag aaaacatgat tcaaaaagga agagaaaagg gaaatattga tgaccttgca
     1801 acagttgctg atgcttcaaa attgccattt aaagacaata tttttaccaa agtatgcata
     1861 tcatttggca tccgcaatat tcctgatatt gataatttcc ttgaagaggt gtacagggtt
     1921 ttacggcctg gtggtacatt cattatttta gaacttaccc gtccacgcta caatatattc
     1981 aggctgctat acttttttta tctttatact attttaccag tgcttggtgg aatcattgct
     2041 ggaagtttta atgcctatag gtatctttca aaaaccatac acacatttat tgaccctgtt
     2101 acattgacag ccatgcagga aaaacatggc tttatcgatg cggtgactca tgagcaattg
     2161 gcacaagcct a
//
LOCUS       DAOSZP010000406         2170 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_38496_length_2170_cov_3.064775, whole genome shotgun sequence.
ACCESSION   DAOSZP010000406 DAOSZP010000000
VERSION     DAOSZP010000406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2170
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38496_length_2170_cov_3.064775"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..354
                     /locus_tag="PK348_08660"
     CDS             <1..354
                     /locus_tag="PK348_08660"
                     /inference="COORDINATES: protein motif:HMM:NF025933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HXK66323.1"
                     /translation="KTPLHTPVEYTEDEIYNFLCRGFEHIKNQPIKVLISHNPPKNVC
                     DRTIFKTNAGSTAIAQFCLDYNIDLCICGHIHEAYGVAQLGNTTVVNAGTFKSGYYAS
                     ILVALNGVEAAIKRL"
     gene            complement(424..1545)
                     /locus_tag="PK348_08665"
     CDS             complement(424..1545)
                     /locus_tag="PK348_08665"
                     /inference="COORDINATES: protein motif:HMM:NF013046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoin utilization protein AcuC"
                     /protein_id="HXK66324.1"
                     /translation="MKNILFYSEDLAQVEYSPTHPFKPMRAKQMMDLLNRYGLLEDTQ
                     ILEHPFLEEELLYLFHTPDYIQALKTCEHGEFDINLLTYGLGTHDNPIFKGVYSFSLQ
                     ASGGTFLGAKKLVETDATFAFNPIGGFHHAMRDHAEGFCYINDLAIALHYLIKKGFKC
                     AYIDIDVHHGDGVQSAFYDDNRVLTISIHESGQFLFPGTGFENEIGDGPGYGYNVNIP
                     LLKDTDDEIYLYAFEEIVPPLLQSFKPDIVCIQVGGDSHKDDPLAHLNLTSNGYEKVL
                     TLIKQYSGKVLGTGGGGYNLYKTSALWTLAWSTFAGIKPVDNFMGVVGGMMFGPEMDA
                     GSLYDNPYHIGGLTKEQCFSYARDVVDYIKQTVFPIHKI"
     gene            1683..1949
                     /locus_tag="PK348_08670"
     CDS             1683..1949
                     /locus_tag="PK348_08670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66325.1"
                     /translation="MRRHNGAIVISSAGGEEYAYESAQWKNGVFTFSVIEGLTMKKAD
                     RNKDGIITISELMSYVANRTRELTGGRQNPTSRKENVEMDFKIW"
BASE COUNT          709 a          397 c          420 g          644 t
ORIGIN      
        1 aaaacgccgc tgcacactcc tgttgaatac actgaggatg aaatatataa ctttttgtgc
       61 agaggttttg aacatataaa aaatcaacca attaaggtgc ttatatcgca taatccacca
      121 aaaaatgttt gtgacagaac tatcttcaaa accaatgctg gaagtacagc gattgcgcag
      181 ttttgtctgg attataacat agatctatgt atttgcgggc acatacatga agcgtatggt
      241 gtagctcagt taggcaatac caccgtggtt aatgcaggga catttaaaag cggatattat
      301 gcttctattc tggtggcatt gaatggggtt gaggctgcca tcaagcgtct atagaaaaaa
      361 taaaaataaa gatagtcaaa atacggttac ccatatgctg caagtagcgg gcttacactt
      421 gaattatatt ttatgaatgg ggaatactgt ttgttttata taatccacca catcgcgtgc
      481 gtatgaaaaa cattgttctt ttgtcaatcc gccaatgtga taaggattat cgtacagtga
      541 gccagcgtcc atttctggtc caaacatcat gccgccaaca acacccatga aattatccac
      601 aggtttaatg cctgcaaatg tggaccatgc cagtgtccac agtgccgatg ttttatacag
      661 attatatcca ccacctccag ttcccagtac tttgccggag tattgcttta ttagtgttag
      721 tactttttcg tatccgttac tggtaagatt aaggtgcgcc agaggatcat ccttatgact
      781 atcgcccccc acctgaatgc atacaatatc tggtttaaaa ctttgtagga gtggtggtac
      841 aatttcttca aaggcgtaaa ggtaaatttc gtcgtcggta tcttttaaaa gagggatgtt
      901 aacattatac ccgtatcctg gcccatcacc aatttcgttt tcaaaaccgg taccaggaaa
      961 taaaaactgc ccactctcat ggatagatat ggttaatact ctgttgtcat cgtagaaagc
     1021 actctgtaca ccatcaccat ggtgtacatc aatatctatg taggcacatt tgaatccttt
     1081 tttgataaga taatgcaatg ctatggctag atcgttaata taacaaaacc cttcagcatg
     1141 gtcacgcatg gcatgatgaa agccaccaat gggattgaaa gcaaatgttg catcggtttc
     1201 aactaatttc tttgcaccta aaaacgttcc gccagaagcc tgaagggaaa aactgtatac
     1261 tcccttaaag atggggttgt catgagttcc caggccatag gttaacaggt taatatcaaa
     1321 ttcaccatgt tcacaggttt taagtgcctg tatgtaatca ggggtatgaa acaggtacaa
     1381 caattcctct tcaaggaaag gatgttccag tatttgtgta tcttcaagca gcccatatcg
     1441 gtttaataaa tccatcatct gttttgcacg cattggctta aacggatgtg taggtgagta
     1501 ctctacctgg gctagatctt cggaatagaa aagaatattt ttcataacac atcaatcctt
     1561 cataagcaag ctagtgtcac aatgacgtgc agtatataat aaatcaagct attttaacaa
     1621 atccgatttt atttctatta aagaaataat atgtataacc atagaaagaa ttatttgctg
     1681 atttgagaag gcataatgga gcgatagtca tatcgtccgc aggtggggaa gaatatgcgt
     1741 atgaatcggc acaatggaaa aatggagtat tcacgttcag tgttatagaa ggtttaacca
     1801 tgaaaaaagc tgatagaaat aaagatggta ttattacaat ttctgaactc atgagctatg
     1861 ttgctaaccg tacacgcgag ctaactggtg gaaggcaaaa tccaacaagt cgtaaagaaa
     1921 atgtggaaat ggatttcaaa atatggtaga agccagtaac aactgatgtg atgaatatag
     1981 tgttaatgac cagcttgaag gattacagtt cattttatta tgatatctat ccacactgaa
     2041 atacagatac tttgacaaaa aatatatact catcctgctt ttcccagttt agttgctgaa
     2101 aatttttaaa aaaaatcaac cagtcggtaa aaaaattatt gacattttat gattactaaa
     2161 atatattata
//
LOCUS       DAOSZP010000407         2168 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_38538_length_2168_cov_3.635116, whole genome shotgun sequence.
ACCESSION   DAOSZP010000407 DAOSZP010000000
VERSION     DAOSZP010000407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38538_length_2168_cov_3.635116"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="PK348_08675"
     CDS             <1..258
                     /locus_tag="PK348_08675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66326.1"
                     /translation="RESAHINLMQSLEKQCYDTLINVTAQLQGISVTDSFQQCLLQQL
                     KQCIDNKVIVVTYFNEDFSETAVVRIYEKGLQQKLATLQCK"
     gene            279..719
                     /locus_tag="PK348_08680"
     CDS             279..719
                     /locus_tag="PK348_08680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66327.1"
                     /translation="MISYIRLIFFSLLLYLPMICYISSCASKKTVTNIPPQENNALLF
                     EYEKEGFIDNNTFRVIVIIPAEEHYDELSIQQKGQERAFVSLKNYIISRNKVFDSKMH
                     NHLMTTITVYGTLKKRDSTCSTRYCYYYDITKSGLKAEIDTLGK"
     gene            complement(720..1268)
                     /locus_tag="PK348_08685"
     CDS             complement(720..1268)
                     /locus_tag="PK348_08685"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HXK66328.1"
                     /translation="MRKYIRYDKLSAEIKDAIYKYWEAAKQNKPDIALDDAMEDWFLH
                     QFDAFMIAKYHTQGDNMRKHFRLDVEIPIRVVELLIESSKDEAEALELIGTIVNISKG
                     GLYFISDIPLELSSIIRVVIDFRAVDNELTDIEALAMVVRHDRREDNRYGIGVMFSSI
                     YDNGKRNLNIFILKNLSYYLYS"
     gene            complement(1346..1765)
                     /locus_tag="PK348_08690"
     CDS             complement(1346..1765)
                     /locus_tag="PK348_08690"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HXK66329.1"
                     /translation="MSTVELKIKGYHLDLFGRVSNQRFLDFLEDARWSYLDTIGLNYD
                     EFAKRGVFLAVVNINVNFRAPSFLGDVLIIETMVDRIGNRSITVKQKMYNKKTEQIIL
                     DAEVVYVIVDLATGKSIPIDNEMRQLWLELSNKKNTH"
     gene            complement(1841..>2168)
                     /locus_tag="PK348_08695"
     CDS             complement(1841..>2168)
                     /locus_tag="PK348_08695"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HXK66330.1"
                     /translation="NIFETLCLGQIIRYYTNQGINPDIYFFQDAYGHEIDFVIPSGEK
                     LTLIECKWLFPLDKRKEIVSKIKQIIPQEKIKKIVILSSQNEEAALEDKLFLYGCHDP
                     DRFLRD"
BASE COUNT          706 a          419 c          342 g          701 t
ORIGIN      
        1 agagaaagtg cacatattaa tttaatgcaa tcacttgaaa agcaatgtta tgataccctt
       61 attaatgtta ccgcacaatt acagggtata tctgttaccg atagcttcca gcaatgcctg
      121 ctacaacaat taaaacagtg tattgataat aaagtcattg tagttaccta ttttaatgaa
      181 gatttcagtg aaactgcagt tgtgcggata tatgaaaagg ggttgcagca aaaactggca
      241 accttacagt gcaaataaca aggataattg gggttaatat gatttcttat atacgattaa
      301 ttttttttag tctgcttttg tatctgccta tgatttgtta tatatcttca tgtgcctcca
      361 aaaaaacagt taccaacatc ccaccccaag aaaacaatgc tcttcttttt gagtatgaga
      421 aagaaggatt tattgacaac aacacattca gggttatcgt aataattcct gctgaagagc
      481 actatgatga gctatcaata cagcaaaaag gccaggaacg tgcatttgtt tctttaaaaa
      541 attatattat ttctcgaaat aaagtttttg attcaaaaat gcataaccac cttatgacaa
      601 ccattacggt gtatggtact ttgaaaaaaa gggattcaac atgttctaca cggtattgtt
      661 attactatga tattacaaaa agcgggctta aagcagagat tgacacactg gggaaataat
      721 caggaataaa gataatacga aagatttttt aaaataaata tattgaggtt gcgcttgccg
      781 ttatcataaa tactgctgaa cataacgcca ataccgtatc gattatcttc tcgcctgtca
      841 tggcgcacta ccattgccag tgcctcaata tcagttagct cattatcaac cgccctgaaa
      901 tcaataacca ccctgatgat tgaagaaagc tcaagcggaa tgtccgatat aaaataaagc
      961 ccacccttgc tgatattcac aattgtgcct attagctcca gcgcttcggc ttcatctttt
     1021 gaagattcaa taagaagctc caccacccgt ataggtattt caacatccag gcgaaaatgc
     1081 ttgcgcatgt tatcgccctg agtgtgatat ttggcaatca taaatgcatc aaactgatgc
     1141 aaaaaccagt cctccatagc atcatctaat gcaatatcag gtttattttg ttttgctgct
     1201 tcccaatatt tgtatattgc atctttaatc tctgcggaca gcttatcata acggatatat
     1261 tttctcatta tgccctcttc cataatggat actcaacctt gtggaatata atacagtaaa
     1321 gtggtatgca tagcaatcaa attttttaat gtgtattttt tttgttcgat agctccagcc
     1381 acagttgccg catttcatta tctattggta tggatttacc tgttgccaga tccactatga
     1441 catataccac ttcggcatcc aaaattattt gttcggtttt tttattgtac atcttttgct
     1501 taactgttat actgcgattg ccaatcctat ctaccattgt ttcaataatc agcacatcac
     1561 ccagaaacga aggggcgcgg aaattaacat ttatattaac cactgcaagg aatacaccac
     1621 gttttgcaaa ttcatcatag ttcaggccaa tagtatctaa atagctccag cgagcatcct
     1681 ctaaaaagtc cagaaaacgc tgattgctta cgcgaccaaa aaggtccaaa tgatatcctt
     1741 taatttttag ctcaacggta gacatgtcat acctgcttat ggaatccaca ttgtccgttt
     1801 atatttacat acaggaatta ttttggcaat tagtttttta tcaatcacgt aaaaatctat
     1861 caggatcatg acacccataa aggaaaagct tatcttctaa tgcagcttct tcattttgtg
     1921 aactcaaaat cacaattttt tttatttttt cctgtggaat gatctgttta atcttagaaa
     1981 caatttcttt tcgtttatct aaagggaata accatttaca ctcaataagg gtaagttttt
     2041 ctcctgaagg aataacaaaa tcaatttcat gaccatatgc atcttgaaaa aaatatatat
     2101 cgggattaat accctgattg gtataataac gtattatttg ccccaaacac aacgtttcaa
     2161 aaatattt
//
LOCUS       DAOSZP010000408         2157 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_38794_length_2157_cov_2.486204, whole genome shotgun sequence.
ACCESSION   DAOSZP010000408 DAOSZP010000000
VERSION     DAOSZP010000408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2157
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38794_length_2157_cov_2.486204"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            407..>2157
                     /locus_tag="PK348_08700"
     CDS             407..>2157
                     /locus_tag="PK348_08700"
                     /inference="COORDINATES: protein motif:HMM:NF012927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXK66331.1"
                     /translation="MYQLLFTPITINTLEIKNRIAYPALGLLYSYDGTLNDRYYEYFR
                     EKAKGGAGLVTVGPVGIDIAGSGRIALSLASDDRIPDFAKLAQIIKNEGARTFVQLFH
                     AGAYSYSMLTDGIQPVAPSAVYSKYSKQTPREMTLEDIEIVQKAYVDAALRVKEAGFD
                     GVEIIASAGYLITQFLSPITNKRTDKYGGSFENRVRFPREVIEKVRQAVGKDFPISIR
                     MAGNDFVPGSNTSRETPLFAKVYEEAGVDIINVTGGWHEAKVPQLPMELPRAGYAYLA
                     ANIKKAVNVPVMASNRISSPVEAERILRDSMADMVNLGRVLIADPYWPKKAMQGKAKL
                     IRPCVGCNQGCTDTLFSGMPVGCILNACAGYEFERSIAKTSHPLKIMVVGAGPAGLEA
                     AVRAKEAGHEVEIFEKEHEIGGQLFIAAAPPNKQELLEIIRYYKAMIEEYSIPVHLNT
                     AVTIDLINKKKPDHVIVATGAEPIKPPIKGIDSPHVYSAWDILQNNPKLGRNVAVIGG
                     GAVGIETALFIANKGTLSPEVLHFLMFFEAEPVERLKELITQGTSTVTIIEMLPSIGK
                     DVGRSTKWIMKLELEKFG"
BASE COUNT          716 a          349 c          498 g          594 t
ORIGIN      
        1 tatggatgat aacataccct gaagagataa taaacccaac gataaatctt acaaaagaaa
       61 tctcaatacc atgaatggag gatgtactgg tgatatattt tgcaaacacc gttgataaag
      121 aaaaagtaat tgcagaaagg agtacataca gaatgccttt tgtaggaggt gtaatcattg
      181 tataaattcc ataatttacg tattatatgc tgattttaaa aaatataaac taaaatttac
      241 atatatgatt atgcaaatct attaaaacat atagtagcac ataatggcag atatgttggt
      301 aaatgatagt aaaaatattt tattattaat tctgtaaaaa aagttgtaat ataacgtggt
      361 aatgtgataa aatctaaaca taacagtgtt atgtttagga ggctatatgt atcaacttct
      421 tttcacacca ataaccatta atacattgga aatcaaaaac aggattgctt accctgcttt
      481 agggctacta tattcatatg atggtacttt gaatgatcga tactatgaat atttcaggga
      541 aaaagcaaaa ggtggtgcag gccttgttac tgtggggccg gttggaatag atattgcagg
      601 atctggcagg attgccttat cactggcatc agatgacagg attcctgatt ttgcaaaatt
      661 ggcacagata ataaaaaatg aaggtgcgcg gacatttgtg caactgtttc atgctggggc
      721 ttattcctat tcaatgctca cagatggcat acagcctgtt gctccttcag cggtgtattc
      781 aaaatactca aaacagacac ccagggaaat gacactggag gatatagaaa ttgtccagaa
      841 agcatatgtt gatgctgcgc tgcgtgtaaa ggaagctggc tttgacggcg ttgagattat
      901 tgcttctgca gggtatctca ttacacagtt tctatcgccc ataacaaata agcgaacaga
      961 caaatatggc ggatcatttg aaaacagggt gcgattccca cgagaggtga tcgaaaaagt
     1021 taggcaggca gttggaaagg attttcccat aagcatacgg atggcaggga atgattttgt
     1081 tccgggaagt aacacaagca gagaaacacc gttgtttgct aaagtatatg aagaagcagg
     1141 tgtggatatc atcaatgtta ccggtggatg gcatgaagct aaggttcctc aactgcccat
     1201 ggaattgcca cgtgcagggt atgcatatct tgctgccaat attaaaaagg cagtaaatgt
     1261 gccagtaatg gcatccaaca gaatcagctc acctgttgag gctgagagga tacttcgcga
     1321 tagtatggct gatatggtca acctgggaag agtactgatt gctgatccgt attggccaaa
     1381 gaaagctatg cagggaaaag caaaattgat acggccatgc gtgggttgta atcagggttg
     1441 cactgataca ttattcagcg gtatgccagt aggatgtatt ttaaatgcat gcgcggggta
     1501 tgagtttgaa cgctctattg caaaaacttc tcatccgttg aagattatgg ttgtgggtgc
     1561 aggacctgct gggcttgaag cagcagtacg ggcaaaagag gcaggacatg aagtagaaat
     1621 ttttgaaaaa gaacatgaaa ttggtggtca gttgtttata gctgcagcac cacccaataa
     1681 gcaggaatta ctggagatta tccgttacta caaggcaatg atagaagaat attcaatacc
     1741 ggtacatttg aataccgcag taacaattga tcttataaac aaaaagaagc ctgatcatgt
     1801 gattgttgct acaggtgcag aaccaataaa accacccatt aaaggaattg atagcccaca
     1861 tgtatattca gcctgggaca ttttgcaaaa taacccaaaa ttggggcgca atgttgcagt
     1921 gataggtgga ggagctgttg gtatagaaac agcactgttt attgcaaata agggcacttt
     1981 gtcgcctgaa gtcctgcatt ttttgatgtt ttttgaagca gaacctgtgg aacgattgaa
     2041 agagttaata acacagggta ccagtactgt caccattatt gagatgcttc cttctattgg
     2101 taaggatgtt ggcagatcaa caaagtggat aatgaaattg gaattagaaa aatttgg
//
LOCUS       DAOSZP010000409         2155 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_38835_length_2155_cov_2.909524, whole genome shotgun sequence.
ACCESSION   DAOSZP010000409 DAOSZP010000000
VERSION     DAOSZP010000409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2155
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38835_length_2155_cov_2.909524"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1998)
                     /locus_tag="PK348_08705"
     CDS             complement(<1..1998)
                     /locus_tag="PK348_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010527909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HXK66332.1"
                     /translation="MKNIVILFSLMLVFGVLSSQTYPFQNPDLSIDERVQDLISRLTL
                     EEKAALMCDQSDAVPRLGIRKFNWWSEALHGYANNDSVTVFPEPIGMAASFNDELVYQ
                     IFSAVSDEGRAKYHEHLRRGNENKRFLSLAVWTPNINIFRDPRWGRGQETYGEDPYLM
                     TRMGIAVVKGLQGPETSKYRKLLACAKHYAVHSGPEWSRHEINLNNIDPRDLYETYLP
                     AFKALVQQADVRQVMCAYHRLDDEPCCGNRRLLQRILRDDWGYKYIVVSDCGAVTDFF
                     TSHKVSSTPVHAASKAVLAGTDVECVWANYPFKTLPKAVENNLIKVEDVDMSLSRVLR
                     GRFELGDFDPDSLVPWTKIPFSIINNEQHRQLALEMGRQSIVLLQNKNNILPLNKSAV
                     KKIAVIGPNANDAVMLWGNYNGTPIRTITILDGITSKLSSEKILYDKACDLVEDKVTQ
                     SYISRARFENKTGFKATYWNSRNMTGDVVAVQNITNSVKLTTAGDHEFAPGVKLEGFS
                     AKYETEFTADKDEEITFNYGATGYFELFVNGESLAKSYNWRTLPSKAHFKVEAGKKYS
                     IEIHYTQLNNWQANLEFDFGKEVNIDFSGLIDKLKGIDVLIFVGGLSGRLEGEEMPVH
                     YPGFKGGDRTDIELPAVQRNLLKMLKQAGKKVIFVNCSGSAI"
BASE COUNT          644 a          472 c          404 g          635 t
ORIGIN      
        1 aatagccgag cctgagcaat tgacaaaaat tacttttttg ccggcctgct ttaacatttt
       61 aagcaaattg cgctgaacag caggtaattc tatgtcggta cggtcgcctc ctttgaagcc
      121 cggataatga acgggcattt cctcaccctc gagccttccc gacaaaccac ccacgaagat
      181 taatacatca atacctttta gcttatcgat aagcccgctg aaatcaatat ttacttcttt
      241 accaaaatca aactcaaggt tggcctgcca gttgttgagc tgggtatagt gtatttcgat
      301 tgaatatttt ttcccggctt caaccttaaa atgtgccttc gagggaagcg tacgccagtt
      361 atagcttttg gccagcgact cgccattaac aaataattca aaatatcctg ttgcaccata
      421 gttaaaggta atttcttcgt ctttgtcggc agtaaattcg gtttcatatt tggccgaaaa
      481 accttcgagt ttgacgccag gagcaaattc atgatcacca gcagtagtta attttacaga
      541 attggtaata ttttgcacag caaccacatc tcctgtcata tttctgctat tccagtaagt
      601 tgctttaaaa ccagttttat tttcaaaccg agcacgactg atgtagctct gagtaacctt
      661 gtcttccaca aggtcgcacg ccttgtcata aagaattttt tcagatgata attttgaggt
      721 aatgccatct aaaatagtaa tagtgcgtat gggagtacca ttataatttc cccatagcat
      781 taccgcatca ttggcattgg ggcctattac ggcaattttt ttaaccgccg atttattgag
      841 tggaagaata ttgtttttat tctgcaacaa tacaatggac tgacgtccca tttccagagc
      901 aagctgacga tgttgttcat tattaatgat tgaaaaagga attttggtcc aggggacaag
      961 agagtcgggg tcaaaatcgc cgagttcaaa acgtccgcgt aatacacgcg atagactcat
     1021 gtccacatct tctactttaa tcaaattgtt ttccactgct ttaggcaatg ttttaaaagg
     1081 ataattagcc cacacgcatt ctacatcagt tccagcaaga acagcttttg aagcggcatg
     1141 cacaggagtg gatgaaacct tatggctggt aaagaaatca gtaacagccc cacaatccga
     1201 tacgacgata tacttatatc cccagtcgtc gcgcaatatt cgttgcagca acctgcgatt
     1261 gccacaacat ggttcatcgt cgagacgatg gtatgcacac atcacctgac gtacatcagc
     1321 ttgctgaacc aatgccttaa aggcaggtaa ataggtttcg tacaaatcac gtgggtcaat
     1381 attgttgagg ttgatttcgt gacgactcca ttcgggacct gaatgaaccg cataatgttt
     1441 ggcacaggcc agcaacttac gatattttga tgtttcggga ccctgtaaac ctttcaccac
     1501 agcaataccc atgcgggtca ttaggtaagg gtcttcgcca taggtttcct gaccacgtcc
     1561 ccagcgaggg tcacgaaaga tattgatatt gggcgtccaa acagcgagac ttaaaaacct
     1621 tttattttca ttgcctctgc gcaaatgttc gtgatacttt gcacgtcctt cgtccgaaac
     1681 agccgaaaaa atttgataaa ccaactcgtc attaaacgaa gctgccattc caataggttc
     1741 agggaacaca gttacactat cgttattggc ataaccatgc aaagcttcac tccaccagtt
     1801 gaattttcga atacccaacc gtggaacagc atcggactgg tcgcacatca gcgctgcttt
     1861 ttcttcgagc gtaagccgcg agattaaatc ctgcacacgc tcgtcaatac ttaaatcagg
     1921 attttgaaaa ggatatgttt ggctacttaa tacaccaaaa actaacatta aagaaaacaa
     1981 aataacaata tttttcataa taataaaatt taatatttaa agttatgata gaacacccat
     2041 gttagcaatt atatttaatg tttgatttca caagaacatg ggcatttcat ttgttcaaag
     2101 ttttaatttt aaagttaagc tataaattcg taattaatac cttatattct tcagt
//
LOCUS       DAOSZP010000410         2151 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_38923_length_2151_cov_3.558206, whole genome shotgun sequence.
ACCESSION   DAOSZP010000410 DAOSZP010000000
VERSION     DAOSZP010000410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38923_length_2151_cov_3.558206"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="PK348_08710"
     CDS             complement(<1..192)
                     /locus_tag="PK348_08710"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013012778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HXK66333.1"
                     /translation="MNIITRLQQKKPLLSFEVFPPKTPIGEENLFHELHVMVSFKPDF
                     ISVTYGAGGSTREKTLEIAT"
     gene            617..1348
                     /locus_tag="PK348_08715"
     CDS             617..1348
                     /locus_tag="PK348_08715"
                     /inference="COORDINATES: protein motif:HMM:NF016501.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament outer layer protein FlaA"
                     /protein_id="HXK66334.1"
                     /translation="MRREVKAFILSLAIGSLVLIVGGGNIMVQAQEAQQPKKQQTGLV
                     EDWMDDFEACEDWRAASTCPIGETKIRKIPGKPRPIDDNGNEINYPDTITDDNGITHE
                     NKFVLGVKTYYLDRGYDRVEVFPPNEYIIRGKAKEVKVWALGRKFRHTLFIKLRDYKG
                     TIHKIKIGRLDFWGWRELSVVIPGWLPQSPGYALLDKNLHFVSFFVESDLYEVPGTFY
                     FYLDNFRVITDLSEFTGDTSIRDNW"
     gene            1390..>2151
                     /locus_tag="PK348_08720"
     CDS             1390..>2151
                     /locus_tag="PK348_08720"
                     /inference="COORDINATES: protein motif:HMM:NF016501.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament outer layer protein FlaA"
                     /protein_id="HXK66335.1"
                     /translation="MKYTRFIALCVVIASVVFVGVFPEKAQSRIVTNVQPDISAEQYN
                     ALVVEDFETQSDWVLETVPKKHQDPKKDPVPVLEMKYVDGGPADLIPEKWSPLNKGLE
                     KKKCLGIHFKFKYPGFNSVHILPPLEVRWDDPTQKVLTYDSRAGQEVQERAIQLPGRA
                     KAVSVWVHGRGNNYDLEVWIKDYKGDVHILKMGSLNFVGWRPMKAYIPENVPQETNSY
                     PQTRVTKIVRFVVRAKPEAGNEDVYLFFDQIKVLTD"
BASE COUNT          706 a          332 c          465 g          648 t
ORIGIN      
        1 ggttgctatc tctaatgttt tttctctagt agaaccgcca gcaccatagg taacggagat
       61 aaaatcaggt ttaaagctta ccatgacatg tagctcatga aataaatttt cttccccaat
      121 gggagttttt gggggaaaaa cctcaaacga taacagtggc tttttttgct gtaaacgggt
      181 aatgatattc attgtgctct ctcacgtctt cataatggat tgcatgtaag tgttacacac
      241 acagaataaa agaagtagct tctgttttgt accactggag tatattgaaa catgcagaaa
      301 tattcaatta gtgtgacaaa aaaatcaata ataattgtat ataaatcaac cataaataaa
      361 gaaataaata ttaatttctt gacacgcata aagtaatgtt gcatactatc gaagacagtt
      421 atgtttgaaa ataatgtatt atttcaatca tatctgttac cgctaatatt tattatatta
      481 gctatgatat caaattatta ttaaatttct ttactttttt taccgatgat gtttatgata
      541 tcatagtaat atgatagtgg tgaagtagtg aaattgtata tattgttcca taatcttttt
      601 tataaaggag tcgacaatga gaagggaggt caaagctttt atattgtcgc ttgccatcgg
      661 tagtctggta ttaattgtcg gtggaggcaa tataatggtg caggcgcagg aagcgcagca
      721 accaaagaaa cagcaaacag gtttagttga agactggatg gatgattttg aagcatgtga
      781 ggactggcgt gcagcttcaa catgccccat aggtgaaaca aaaatcagga aaatacctgg
      841 aaaaccccgt cctatagatg ataatggaaa tgagatcaac tatcctgata caataactga
      901 tgacaatggt ataactcatg agaataaatt tgttctgggt gttaaaactt attatttaga
      961 taggggatat gacagagttg aggtgttccc gccaaatgaa tatatcatcc gaggaaaagc
     1021 taaagaagtt aaagtgtggg ctttaggtag aaaattcagg catacccttt ttatcaaact
     1081 tcgggattat aaagggacaa ttcataaaat taaaatagga aggcttgatt tctggggatg
     1141 gagggaatta tctgttgtaa tcccgggctg gttgcctcag agtccaggat atgcattact
     1201 ggacaagaac ctgcatttcg tttccttttt tgttgaaagt gatctttatg aggttcctgg
     1261 gacattttat ttctatcttg ataatttcag agtcattacc gatctgtctg aattcactgg
     1321 tgatacaagc atcagagata actggtaaaa aagcatgatg accaaatatg agacctcgga
     1381 ggcattaata tgaaatatac acgatttatt gcactatgtg ttgtgatagc aagtgtggtg
     1441 tttgtagggg ttttccctga aaaagcacaa tcaagaattg ttactaatgt tcagcctgac
     1501 ataagtgctg agcaatacaa tgcacttgtt gtggaagatt ttgaaacaca gtcagattgg
     1561 gtattggaga cagttcctaa aaaacatcag gatcccaaaa aagatcctgt accagtactg
     1621 gaaatgaaat atgttgatgg aggaccagct gacctgattc cggaaaagtg gagccctctc
     1681 aacaagggat tggaaaagaa gaaatgttta ggcattcact ttaagtttaa atatcccgga
     1741 tttaattctg ttcatatatt accgcctttg gaagttcgct gggatgatcc aactcagaaa
     1801 gtattaacat atgactcccg tgctggtcag gaagttcagg aacgtgctat acagcttcca
     1861 ggaagagcaa aagcggtatc tgtatgggta catggcagag gaaataatta tgacctggaa
     1921 gtttggataa aggattataa aggcgatgtg catatattaa agatgggttc gctgaatttt
     1981 gtaggctgga ggccaatgaa ggcatatatc cctgagaatg tgcctcagga aaccaattcc
     2041 tatcctcaga cacgggttac caaaattgta cgttttgtag tcagggcaaa acctgaagcc
     2101 ggcaatgagg atgtatatct gttttttgat cagatcaaag tattaactga t
//
LOCUS       DAOSZP010000411         2150 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_38958_length_2150_cov_2.545585, whole genome shotgun sequence.
ACCESSION   DAOSZP010000411 DAOSZP010000000
VERSION     DAOSZP010000411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2150
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38958_length_2150_cov_2.545585"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..779)
                     /gene="panB"
                     /locus_tag="PK348_08725"
     CDS             complement(<1..779)
                     /gene="panB"
                     /locus_tag="PK348_08725"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012676320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HXK66336.1"
                     /translation="MKNIKDFAKMKQSGEPIAMITCYDYAFARVVSETDIDVILVGDS
                     LGMVVAGFDTTIPVTLDQMIYHSSMVRRGAPQTFIVTDLPFMSYHVSIEDTMRNCGRI
                     MKECGANAVKLEGGRDFVPVVEALTKASIPVMGHLGLTPQSVHKLGGYSVQGKDEEQR
                     KIIMEDALLLQKAGAFALVLEMVPEQLAKEITEALDIPTIGIGAGRYCNGQVLVINDM
                     LGLNKGFNPKFLKRYADLYTTSQQAIQTYCKEVKEHIFPAEG"
     gene            complement(776..1237)
                     /gene="folK"
                     /locus_tag="PK348_08730"
     CDS             complement(776..1237)
                     /gene="folK"
                     /locus_tag="PK348_08730"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HXK66337.1"
                     /translation="MEAYAYILLGSNMGDSYRYIALAIEKLSTLEKTNIVKQSSVIVT
                     SPLEYTQQPDFVNTVVALLTQLPPHTLLEKLQKIEYSLGRERTIPKGPRTIDCDILLY
                     DDMVCNTPELTLPHPQIYTRPFAAQLLLEINNDIIDPLSHKPLREVVLCHQ"
     gene            complement(1237..2052)
                     /locus_tag="PK348_08735"
     CDS             complement(1237..2052)
                     /locus_tag="PK348_08735"
                     /inference="COORDINATES: protein motif:HMM:NF013828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXK66338.1"
                     /translation="MPTKTQKNDTKSIFCAFCEHTGIVRNPHFEEIGQEPLVPCPRCV
                     LTKCQCGGQPPYYIEKDGTISDCYCREIRMKINRIKAIYLRSGIDKKYQWRFFNSFEA
                     NSKLASNAKNVAYDIVSKFPDVRKGLFLWGNPGTGKTLLSSIILTELIIRHAIEGRFI
                     KISRTFFNKLRATFAEGSATYGTAQEIEYELENADVLVVDDFGVQRDSPWEQETLYNL
                     VDARYEAEKFTIFTSNVDPHRALSEIAGGRVLSRIKEMCTILELTGPDYREKL"
BASE COUNT          574 a          466 c          447 g          663 t
ORIGIN      
        1 ccttctgcag ggaatatgtg ttcttttact tctttgcagt acgtttgtat tgcctgctgt
       61 gatgtggtat aaaggtcagc atagcgcttt aagaattttg gattaaatcc tttgtttaag
      121 cctaacatat cgttaattac aaggacctgt ccattgcagt aacgacctgc accaatgcca
      181 atggtgggta tatcaagcgc ttcagttatt tcttttgcaa gctgctcggg taccatttca
      241 agcactaacg caaatgctcc cgctttttgt aacagcaacg catcttccat gatgatttta
      301 cgctgctctt catccttccc ctgaacagaa tatccgccta atttatgaac tgactgcggt
      361 gtaagcccta aatgccccat aaccggtatt gaagctttgg tcagtgcttc aacaacagga
      421 acaaaatccc ttccgccttc taatttaacg gcattggcac cgcattcttt cataatcctg
      481 ccacagttgc gcatggtatc ttcaattgat acgtggtagc tcatgaacgg gaggtcagtt
      541 actataaagg tttgtggtgc accgcggcgt accatgctgc tgtgataaat catctggtca
      601 agggttactg gtatggtggt atcaaagccc gcaacaacca tccccaatga atcgccaacc
      661 agtatgacat caatatcagt ttcgctgaca acacgtgcaa aggcataatc ataacaggtt
      721 atcatggcaa tgggctcgcc tgactgtttc atctttgcaa aatctttgat gttttttatt
      781 gatgacatag tactacctcc cgtaatggtt tgtgagaaag cggatcaatg atatcgttgt
      841 ttatttccag caatagctgt gctgcaaatg gtcgtgtgta gatttgtgga tgaggcaggg
      901 taagttccgg tgtgttacac accatatcat cgtaaagcaa aatatcgcaa tcaatagtgc
      961 gggggccttt agggatggtt ctttcccttc ccagtgaata ctctattttt tgaagctttt
     1021 caagtagcgt gtgtggtggc agttgcgtga gcagcgcaac aactgtattg acaaaatcgg
     1081 gttgctgggt atattctaag ggactggtta ctattacgct gctttgcttt acaatgttgg
     1141 ttttttccag tgtggacagt ttttcaatag ctaaggcaat ataccggtaa ctatcgccca
     1201 tattgcttcc cagtaaaatg tatgcatacg cttccattat aatttttccc tgtaatcggg
     1261 acctgttagc tctaaaattg tgcacatttc cttaatgcgc gacaatactc tgccgccagc
     1321 aatttcagac agtgcacgat gggggtcaac gttggatgta aatatggtaa atttttcggc
     1381 ctcgtatcgc gcatcaacca gattgtacag ggtttcctgc tcccaggggg aatcgcgctg
     1441 tacaccaaaa tcatccacta caagcacatc ggcgttttct aattcatact caatttcctg
     1501 tgccgtaccg taggtggcag aaccttcggc aaacgttgca cgcagtttat taaagaatgt
     1561 acgtgaaatt ttaataaagc gcccttctat ggcatggcgt atgataagct cagttaatat
     1621 aatagacgat agcaaagttt tccccgttcc agggttgccc cataaaaaaa ggcccttgcg
     1681 tacatccgga aattttgaaa caatatcgta agcaacattt tttgcattgg atgctaattt
     1741 tgaattggct tcaaaggaat taaaaaatcg ccactgatat tttttgtcaa tacctgaacg
     1801 cagatatatt gcttttatcc ggttaatctt cattctaatt tcacggcaat agcaatcgga
     1861 tattgtgccg tctttttcta tatagtacgg aggctgtccg ccgcactggc atttggtcaa
     1921 cacacagcgg ggacagggta ccagcggttc ctggccaatc tcttcaaaat ggggattgcg
     1981 cacaataccg gtatgctcac agaatgcgca gaagattgat ttggtatcat ttttttgagt
     2041 ctttgttggc attaaaacct ctcgctacat caatgttcaa cgttcacgaa cactttaaca
     2101 ctacaaacag tctatataat ttcaatgata atttcaaata aataatgtaa
//
LOCUS       DAOSZP010000412         2145 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_39072_length_2145_cov_4.478947, whole genome shotgun sequence.
ACCESSION   DAOSZP010000412 DAOSZP010000000
VERSION     DAOSZP010000412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2145
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39072_length_2145_cov_4.478947"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1177)
                     /locus_tag="PK348_08740"
     CDS             complement(<1..1177)
                     /locus_tag="PK348_08740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66339.1"
                     /translation="MKKFFYYIVLFVAIVTVYGCTGKNALFDELSGSRLTVIIKGTFE
                     STSDSTSVNGWNTTVPGTKIDDSVNDFVTDSSESFPTTFMVDIAEIRLAKPDGSSDDK
                     VGNYRQVFTSQIDDSDPFFNGTGIKLKCDDIKPGKQYGMVKLYIRKLIFDNATQWYID
                     GSNNIVQDNAPEVIFHEEEVNGFDFNQLMVNTYYDYLKENYQDINRVFPLEIPIDGGL
                     IVDEDEEVTLEIRLVIKNFIKLYEYDFTNDDGYGAAYHYWAPSDWLRDVQPDDVFIGG
                     NLLGVARAYRKNAVATVSGNTGSNSGYVIAIPRYDMYGNEQNIGDYFLDDDDPLNWLR
                     PTVYPPATPSVKVGTVEGYLDYYLRYEQYKIEYNDFVDAVNDGSYETLWNSYDGYKHS
                     "
     gene            complement(1185..1754)
                     /locus_tag="PK348_08745"
     CDS             complement(1185..1754)
                     /locus_tag="PK348_08745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66340.1"
                     /translation="MKLKLSWLFVAFTLLCSANDVFAQRKKADYFLKPQTGIWFGPVT
                     PVFSTADDVDTALWGGGFVRFNILSLPLKIGFDSSYEHFKSEGVKELTVIPVYGNILW
                     RLPFDMPLAFQLKAGAGSAKVTIEPEGLQQWDPLGMLGFELSFPAGKWINIGLRIDYL
                     FIYEGYIQGASKNGHIINTGLTLYLNIGM"
     gene            complement(1942..>2145)
                     /locus_tag="PK348_08750"
     CDS             complement(1942..>2145)
                     /locus_tag="PK348_08750"
                     /inference="COORDINATES: protein motif:HMM:NF013575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HXK66341.1"
                     /translation="KGNAFLHNMVRILVGTILQLMKEGKSPDTMQTILKQCDRTYSGI
                     TAPAYGLYLKKVYYPEKYNFLNV"
BASE COUNT          636 a          487 c          347 g          675 t
ORIGIN      
        1 atgaatgttt atacccatca taactattcc ataatgtttc atagctacca tcattaacag
       61 catcaacaaa gtcattgtat tcaatcttat actgctcata acgcaaatag taatctaaat
      121 acccttcaac cgtcccaact ttaactgaag gtgttgcagg cggatataca gttggtctta
      181 gccaatttag agggtcatca tcatctaaaa aataatcacc aatattctgt tcatttccat
      241 acatatcata acgtgggatt gctattacgt aaccactgtt actaccagtg tttccactta
      301 ctgttgcaac cgcattcttt ctatatgcac gggcaactcc taataaattg cctcctatga
      361 atacatcatc cggttgcaca tccctgagcc agtcacttgg cgcccagtaa tgataggcag
      421 caccatagcc atcatcgttt gtaaaatcat actcatacaa tttaataaag ttcttgatta
      481 caaggcgtat ctccagtgtt acttcttcgt cctcatccac aattaagccg ccatcaatag
      541 gtatctccag cgggaacaca cggtttatat cctgatagtt ttccttcagg taatcatagt
      601 atgtatttac cataagctga ttaaaatcaa aaccatttac ttcctcttcg tggaatataa
      661 cttcaggtgc attatcctga acaatattat tggatccatc tatgtaccat tgcgttgcat
      721 tatcaaagat aagcttgcgt atatacagtt ttaccattcc gtactgtttt ccgggcttta
      781 tgtcatcaca ttttagttta ataccggtcc cattgaaaaa cgggtcactg tcatctattt
      841 gtgatgtaaa tacctgccgg taatttccta ccttatcgtc gctgctgcca tctggctttg
      901 caaggcgtat ctcggcaata tcaaccataa aggtagtggg aaagctttca gagctatcgg
      961 ttacaaaatc attaacggaa tcatctattt tagttccggg aacggttgta ttccacccat
     1021 taacactagt gctatcactt gttgactcaa atgtaccttt gattattact gtcaggcggc
     1081 ttccggacag ctcatcaaaa agagcatttt tgccggtgca cccatagaca gtaactatcg
     1141 ccacaaataa tactatgtaa taaaaaaatt ttttcatcgt tatattacat cccaatattc
     1201 agataaagtg tcaatccggt attaatgatg tgcccgtttt tgctggcacc ttgtatatag
     1261 ccttcataga tgaaaagata atcaatcctg agtccaatgt taatccattt gccggcagga
     1321 aacgatagct caaatccaag catccccaga gggtcccatt gctgcagacc ttcaggctca
     1381 atggtaacct tagcactacc tgcacctgcc tttagctgaa atgcaagcgg catgtcaaat
     1441 ggcaaacgcc acaaaatatt gccgtatacc ggaattaccg ttagctcctt aacgccttca
     1501 gatttgaaat gctcatacga agaatcaaac ccaatcttaa gaggcagcga caatatatta
     1561 aatcgcacaa acccgccacc ccacagcgca gtatcaacat catctgctgt tgaaaatacc
     1621 ggagtaaccg gaccaaacca gatccctgtc tggggtttga ggaagtaatc agcctttttg
     1681 cgctgtgcaa aaacatcatt ggcactgcat agcaatgtaa acgcaacaaa gagccacgat
     1741 aattttaact tcacacgtat caccttttct ttttgtttca ctaacaattg ttgtgatatc
     1801 actatgtgtc aataattatt ttttacttac tgtatataag aacgtgtaaa tccacttcat
     1861 ttctcaagcc ggggatgttt ttcttttcat atattcatac tatacatagt ataaaaatat
     1921 ggaaatatac cgtgtttttt gttacacatt taaaaaatta tatttttcag gataatatac
     1981 ttttttaaga tacagcccat acgcaggggc tgttattccg ctataggttc tgtcgcactg
     2041 ctttagtatt gtttgcattg tgtcagggct tttaccttct ttcataagct gcaatattgt
     2101 acctaccagt atccgtacca tattatgcaa aaaggcattg ccctt
//
LOCUS       DAOSZP010000413         2134 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_39329_length_2134_cov_3.426647, whole genome shotgun sequence.
ACCESSION   DAOSZP010000413 DAOSZP010000000
VERSION     DAOSZP010000413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39329_length_2134_cov_3.426647"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..498)
                     /locus_tag="PK348_08755"
     CDS             complement(<1..498)
                     /locus_tag="PK348_08755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66342.1"
                     /translation="MDGKVKRSIIINRYYQLKTGFGVAGLFALCISILFIITGSFVMW
                     NNRSISRAMAHQLDLQNVQDDILKSIEMLSQYGNRKNLIFEAYKAIGDLQKNASALKE
                     SNELLTRSIAINRYLMWINGVLGITLVVVTFIIVVRRTHRVAGPMFLLKRYMNEIIEG
                     KVPEIR"
     gene            complement(517..>2134)
                     /locus_tag="PK348_08760"
     CDS             complement(517..>2134)
                     /locus_tag="PK348_08760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HXK66343.1"
                     /translation="CQNIYDTSPNIIDAARFAAHFYYSIPWYADLVEFLQSTDNVQLQ
                     TVAGNFANCVLKKVMPLANIHSGLSIMFTTKTHESWYISNLYSMDFVRDYSWDEFLQD
                     KHIGLYIDPNELFNNEPDGYTVMLVDGNTDSITINCQNYFHDPYDADIYSIDFNYRGG
                     IYRLTDNTVNTINSNNDRWQSITASQDGAKLAAAVYDGYIYTSGDGGTTWVQRNTPKS
                     WTNITGSQDGTRLAATVYGGYIYTSGDGGTTWTQRSIGPKNWISVACSQDGSKMVAAA
                     YNDYIYISTSGGNSWTSIANPGQKNWSGVAIAYDGTKIAATSYDGIYIITNQSGSWVA
                     SDIYNLSANWISIAVCSIGNDFIIVAAQNPGYIYLSKVVNGVRVWTCLTQAGRRNWKS
                     VSISPDSIRIIAIADNTIFESVDQGQTWYVRNDAGMHRWNFVTYAWNQNALALVSSPA
                     NSAVNGLLTVAVNNVPENCEYIVRIADDNGNILNESDRSGPGGTITLQQYIQTNVFRH
                     YFISLIPVNVSGWSMNTKEQYAVQFNYQKN"
BASE COUNT          640 a          470 c          334 g          690 t
ORIGIN      
        1 gcgtatttca ggtactttcc cttcaataat ttcattcata tatcgtttta ataaaaacat
       61 tggaccagct accctgtgtg tgcgccttac aacaataata aacgtaacaa caacaagtgt
      121 tattcccagt acaccattaa tccacataag gtatctatta atggcaatac tccttgttaa
      181 cagttcattg ctttctttca atgcactggc atttttttga agatccccta tagctttgta
      241 ggcctcaaaa attaaatttt ttctattgcc atattgtgat aacatttcaa tggacttcaa
      301 gatatcatcc tggacattct gcaaatccag ctgatgagcc attgcccgtg aaatagagcg
      361 gttgttccac attacaaaac tacctgtgat aataaaaaga atactgatac acagggcaaa
      421 caaacctgct acaccaaatc ctgttttcaa ttgataatat ctgtttatga ttatgcttct
      481 tttaactttc ccatccataa gcttcccagt aaaaacctaa ttcttttgat aattgaattg
      541 cacggcatac tgctcttttg tattcattga ccatcctgat acatttaccg gtattaatga
      601 aataaagtaa tgcctgaata catttgtctg tatatattgc tgcaaagtaa tggtgccacc
      661 aggacctgat ctatccgatt cgttgagtat gtttccattg tcatcggcaa ttcgcactat
      721 atattcacag ttttccggaa cattattcac agcaactgtt aaaaggccat taacagcgct
      781 atttgcaggt gagctcacca gagcaagcgc attctgattc catgcatacg taacaaagtt
      841 ccacctatgc ataccagcat cattccgtac ataccacgtt tgaccctgat caactgattc
      901 aaaaatagta ttatcagcaa tagcaatgat tctgatgcta tccggtgata tggatacact
      961 tttccagttt ctgcgtcccg cctgggttaa acaagtccat acccgtacac catttactac
     1021 ctttgataaa tatatataac cagggttctg tgctgctaca ataataaaat cattgccaat
     1081 actacataca gcaatgctaa tccagtttgc agataaatta taaatatcag atgctaccca
     1141 acttccactt tggtttgtaa taatatatat tccatcataa cttgttgcag caatcttcgt
     1201 gccatcatac gctatagcca cacccgacca attcttttga ccgggatttg ctatgcttgt
     1261 ccagctattt ccgccgctgg tagaaatata tatgtaatca ttatatgccg cagcaaccat
     1321 ttttgaccca tcctgtgagc atgctacact tatccagttt ttagggccta tgctacgttg
     1381 cgtccaggtt gtaccaccat cacctgaagt atagatataa ccaccataaa ccgttgcagc
     1441 taaccttgtt ccatcctgtg aacctgttat attcgtccag ctttttggtg tattacgctg
     1501 tacccaggtt gtaccaccat cacctgaagt ataaatataa ccatcataaa ctgctgcagc
     1561 caactttgct ccatcctgcg atgcggtaat agattgccac ctgtcattgt tactgttgat
     1621 agtatttaca gtattatcag ttaacctgta tatcccaccc cggtaattaa aatctattga
     1681 atagatatca gcatcataag gatcatgaaa ataattttgg cagtttatgg ttatactatc
     1741 tgtattccca tccacaagca tgactgtata gccatccggt tcgttgttga atagttcatt
     1801 gggatctata tacaatccaa tatgtttatc ctgtaaaaat tcatcccagc tataatctct
     1861 tacaaaatcc attgaatata aattggatat ataccagctt tcatgtgttt ttgttgtaaa
     1921 cattatggaa agacccgaat ggatatttgc taacggcata acctttttaa gcacacagtt
     1981 tgcaaaatta cctgcaacag tttgcaactg gacattgtct gttgactgta aaaattctac
     2041 caggtcagca taccatggaa tggaatagta aaaatgtgca gcaaagcggg cagcatctat
     2101 tatatttggg cttgtatcat atatattctg gcat
//
LOCUS       DAOSZP010000414         2129 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_39449_length_2129_cov_2.719383, whole genome shotgun sequence.
ACCESSION   DAOSZP010000414 DAOSZP010000000
VERSION     DAOSZP010000414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2129
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39449_length_2129_cov_2.719383"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            32..178
                     /locus_tag="PK348_08765"
     CDS             32..178
                     /locus_tag="PK348_08765"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HXK66344.1"
                     /translation="MSRPTLSKIEQGEFVNVSVRALDKILVALGYELSMQPGNIVGLP
                     PLTG"
     gene            230..1399
                     /locus_tag="PK348_08770"
     CDS             230..1399
                     /locus_tag="PK348_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015024465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXK66345.1"
                     /translation="MIKRKIEKTLKELAKQYPVVTITGPRQSGKTTLCRYVFKHKNYV
                     NLEAPDIRMFALEDPRGFLKQHGNNVIIDEIQRVPQLISYIQTIVDDNPESGQFILTG
                     SSHFGIMNGISQSLAGRTAIITLLPFSYEEIQHIVPCNIDRLLYTGFYPRIHDKKLNP
                     TQALADYVATYVERDLRQLAAIKDLYTFEKFLRLCAGRVGQLLNMQSLASDVGVSHTT
                     IRAWISILEASYIIFLLQPWYGNVAKRLIKSPKLYFYDVGLATYLLGIENEKQMQRDP
                     LRGNLFENMVIAEIIKYRFNRGKRSNIFFYRDSKGTEVDVLYQYGRNIFPIEIKAGAT
                     VVDDFFKNFNAVCKYIDCEKGAVIYGGTEIQKRTGVDVIPFVKIPDLLQQIDKNQ"
     assembly_gap    1535..1544
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1589..>2129
                     /locus_tag="PK348_08775"
     CDS             1589..>2129
                     /locus_tag="PK348_08775"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002729728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HXK66346.1"
                     /translation="MKRALITGITGQDGAYLAELLLQKGYTVIGGFRRLSTPNFWRLE
                     ELNILDKIVMVEIDMLDAGNLVRVIEKYKPDEVYNLAAQSFVAHSFANPVLTGNVTGL
                     GVTNILEAIRIVNKDIKFYQASTSELFGKAQETPQKETTPFYPRSPYAVAKLYGHWMT
                     INYREAYGIFASTGILFNHE"
BASE COUNT          711 a          350 c          448 g          610 t
ORIGIN      
        1 aatcacacag ggaatactgt gcaaaacagg tatcagcaga ccaacgttat caaaaattga
       61 acaaggtgaa tttgttaatg tgagtgtcag agcacttgat aaaattcttg tggctctggg
      121 gtatgaacta tcgatgcaac caggaaatat cgttggcctg ccccccctta caggatgatt
      181 aattgaaaat tatacttgca atttgcacca accgctgtaa actatagcta tgattaaacg
      241 aaagatagaa aaaacgctta aagagcttgc aaaacaatat ccggttgtaa ctattacggg
      301 gccccgacaa agcggaaaaa caacattatg cagatatgta tttaaacata aaaactatgt
      361 aaatttagaa gctcctgaca taagaatgtt tgcacttgaa gatccacgag ggtttctaaa
      421 acagcatgga aataatgtta ttattgatga aatacaaaga gtgccacagc ttatatcata
      481 tatacagaca atagttgatg ataatcctga atccggacag tttatactga ctgggagcag
      541 ccattttggg attatgaatg ggataagtca gtcattagcc ggccgaacag caataataac
      601 attattgccg ttcagttatg aagaaataca gcatatcgtc ccctgtaaca ttgacagatt
      661 gctatataca ggattttatc ctagaataca cgataagaaa ttgaatccaa cacaggcact
      721 ggccgattat gttgcaacat atgtagaaag ggatttaaga caactggctg ctatcaagga
      781 cctttacaca tttgaaaaat ttttgcgttt atgtgcaggt agggtagggc agcttttaaa
      841 tatgcagagt ctggcaagtg atgttggcgt atcgcacaca actatacgtg cgtggatatc
      901 aatacttgaa gccagttata ttatattttt gcttcaacca tggtatggca atgtagcaaa
      961 acgtttgata aaatcaccaa agctatattt ttatgatgtt ggactggcaa cctatctcct
     1021 tggtattgaa aatgaaaaac agatgcaacg cgatccatta cggggcaatc tttttgaaaa
     1081 tatggtcata gctgaaatta taaaatatag attcaaccgt ggtaaaagga gcaatatatt
     1141 tttttatcgg gatagcaaag gtacagaggt tgatgtactg taccaatatg gaaggaatat
     1201 attccccatt gaaataaaag caggcgctac agttgtggat gattttttta aaaattttaa
     1261 tgcagtatgt aagtatattg attgtgaaaa aggtgcagtt atatatggtg gcactgaaat
     1321 tcaaaaaaga actggcgttg atgttattcc gtttgttaaa atccctgatt tattacaaca
     1381 aatagataaa aatcaataaa ctattgatgt ctggaatcac tgtaagttat tgacaataat
     1441 tgtgtatatt gtagtgtacg atagtggttg taaatggtca aagaaaccgg atggatgatg
     1501 tagctattta aagagcagtg atattaacct tgtcnnnnnn nnnngcgcgg aattacgatt
     1561 ttcaggaatt atactattga gggaatatat gaaacgagca ctcattacag gcattactgg
     1621 gcaggatggt gcatatttag cagagttgct tttgcagaag ggatataccg ttattggcgg
     1681 gttcaggcgg ttgagcactc caaacttctg gcgattagag gagcttaata ttcttgataa
     1741 gattgtcatg gttgaaatag atatgcttga tgctggcaac ctggtgcgcg tcatagaaaa
     1801 atataaaccg gatgaagtgt ataatttagc tgcgcagagt tttgtggcac actcatttgc
     1861 aaatcctgtt cttacgggta atgtcaccgg ccttggtgta accaatattc tggaagcaat
     1921 ccgaatagtg aataaagaca taaagtttta tcaggcatca acatccgagc tttttggcaa
     1981 ggcacaggaa actccacaaa aagaaacaac accattttat ccccgcagtc cctacgctgt
     2041 ggcaaagcta tacggtcact ggatgacaat aaactatcgc gaagcctatg gcatttttgc
     2101 ttccacggga attcttttta accatgaat
//
LOCUS       DAOSZP010000415         2124 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_39540_length_2124_cov_3.048333, whole genome shotgun sequence.
ACCESSION   DAOSZP010000415 DAOSZP010000000
VERSION     DAOSZP010000415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39540_length_2124_cov_3.048333"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            47..301
                     /locus_tag="PK348_08780"
     CDS             47..301
                     /locus_tag="PK348_08780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66347.1"
                     /translation="MFKNTVLERYKNIYPGCDYLLFVVYNPSRTSCWARAIDIKTKEL
                     IFMHSNIEASEMNANLQKTTRQLITIIQTPTALPQKSKKK"
     gene            complement(309..887)
                     /locus_tag="PK348_08785"
     CDS             complement(309..887)
                     /locus_tag="PK348_08785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66348.1"
                     /translation="MKKIIVMIITVLSSTTIAMANVYVDGLLMINNTGDAKSQSGFGL
                     KIATSIRDDINIFFRQTYSSTTEDPNTINETEYMQIQGFLGGEYVFFIQQFPVAITLS
                     AAAGMSRTEVNPKDDFSGIKDLGENGIGYGLWVGTQWLLTQRVTPFIEIGYSKSMYTT
                     DFKNASIGGLQFLAGIRFTVWGKSKSLSNDYE"
     gene            1177..>2124
                     /locus_tag="PK348_08790"
     CDS             1177..>2124
                     /locus_tag="PK348_08790"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HXK66349.1"
                     /translation="MKNRILGILFCFICIHAVSVAFAEQPDSITAIINEEQNKEHSDA
                     LLQMLQFIDSLYDNLQSRVKNGEKIVIFFDPAHGIDDDGKWEGEKTGRLSCTGLPEEY
                     YSLALSRQLYSLLKKNPYIQVVSTQDYIDALEGKTDTYNNITFRETVELAKNARAVLV
                     ISEHLNNTASIFKAYGLANVKGIHITYSNGKAYLTHIREEHKGFLTLYNKFDASGFSY
                     RVASNVKEKLLAYNLRVNSWNYGTVADDRFTYFVNYPISVIFESGFISNPDDEKILSN
                     PEYQKLIAQAHYDAILETIANVFGIDISGFWGPRIVDTAD"
BASE COUNT          708 a          367 c          382 g          667 t
ORIGIN      
        1 ttaaagatat ataaaaatat atgaaatata tgacttacat tataaagtgt ttaaaaatac
       61 agtacttgaa agatacaaaa atatatatcc aggatgtgat tatttactgt ttgttgtgta
      121 taatccttcg cgtacaagct gctgggcgcg ggctattgac ataaaaacaa aagaattgat
      181 atttatgcac tcaaatattg aagcatctga aatgaatgcc aatctccaga aaaccacacg
      241 tcagcttatc acgattattc agacaccaac agcactgcct caaaaatcaa agaagaaata
      301 agacacattt attcgtaatc gttagataac gatttgcttt tgccccatac agtaaaacga
      361 attccagcta aaaactgcaa tccacctata ctggcattct taaaatcagt tgtatacatt
      421 gattttgaat aaccaatttc aataaatggt gtaactcgct gtgtaagcag ccactgagta
      481 cctacccata aaccgtatcc aataccgttt tcacctaaat ctttgatgcc tgagaagtca
      541 tcctttgggt ttacttctgt gcgtgacatt ccagcagcag cactgagtgt gattgctaca
      601 ggaaactgct gaataaaaaa tacatattcg ccacccagga aaccctgaat ttgcatatat
      661 tcagtttcat taatggtgtt tggatcttcc gtagtagatg aatatgtttg tctgaaaaat
      721 atatttatgt catctctaat acttgttgcg attttcagcc caaagccgct ctgcgacttt
      781 gcgtcacctg tattattgat catgagcaat ccatcaacat atacattagc cattgctatt
      841 gttgtactgg aaagcacggt aattatcata acaattattt ttttcacagt aacctccatt
      901 gaatcatggc tcctattaca tcaaaagtgc ttacatagta actatcgccc aattttaatt
      961 gtttctaagt atatacttat gaatatgttt atgtcaatat aaaaaaattg aaattctgca
     1021 attcttttaa tgtatggtga tttcattggt aaaatataat ttgtttatac tataaaaaag
     1081 acttgcagta gtacaatgtt tgagaataat ttaataaaac actctatatg ttcgaaatta
     1141 taaatatagc ttacagagga caaggtttgg gttttaatga aaaacagaat actgggcata
     1201 ttgttttgtt ttatttgcat tcacgctgtt tcggttgcat ttgctgagca gcctgatagt
     1261 atcactgcaa ttataaatga agagcaaaat aaagaacatt cagatgcatt gctgcagatg
     1321 ctgcagttta tagatagttt atatgataat ctgcaaagcc gggtgaagaa tggtgaaaag
     1381 atagtaatat tttttgaccc cgcacatggt atagatgatg atggcaaatg ggaaggcgaa
     1441 aaaacaggaa ggctaagctg tacagggtta cctgaggaat attattctct tgcactttca
     1501 cgccagctat acagtctttt aaagaaaaat ccatatattc aggtggtaag cacacaggat
     1561 tatattgatg cccttgaagg aaagactgat acgtataaca acatcacctt cagggaaaca
     1621 gtggaactgg caaaaaatgc cagggctgta cttgttattt ctgaacatct taataataca
     1681 gcatcaatat tcaaagcgta tgggcttgca aatgtaaaag gcatacatat tacgtacagt
     1741 aacgggaaag cctaccttac tcatatacgg gaagagcata aagggttttt aacgctgtat
     1801 aataagtttg atgccagtgg tttttcgtac agggttgcaa gcaacgttaa ggaaaaactt
     1861 ttggcttata atttgcgtgt taacagctgg aattatggca ccgtagcgga tgaccggttt
     1921 acgtattttg tcaattaccc catatctgtt atctttgaat ccggtttcat atcaaacccg
     1981 gatgatgaaa aaattttaag taaccctgag tatcaaaaac tcattgcaca agcacattat
     2041 gatgcaattc ttgaaaccat tgctaatgtc tttggcattg atatttctgg tttctgggga
     2101 ccacggattg ttgacactgc tgat
//
LOCUS       DAOSZP010000416         2123 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_39556_length_2123_cov_4.397969, whole genome shotgun sequence.
ACCESSION   DAOSZP010000416 DAOSZP010000000
VERSION     DAOSZP010000416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39556_length_2123_cov_4.397969"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(167..>2123)
                     /locus_tag="PK348_08795"
     CDS             complement(167..>2123)
                     /locus_tag="PK348_08795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66350.1"
                     /translation="LIDWRSNHNALHTLWIVPLLFHEFTDNGYRYYVPLYCSPRRTAY
                     EGVSFGLFHFHRWQPDMDRLWIWLYYHSKHYAINNAPQKNGVEQYYIHFLPIYFSWRN
                     DTSKGNILLPFVYNYSDTNTTVHINLTGYARKTYTGIFKPDVGVNLSQSEGRKYLDTD
                     VSWLYAMWSFSSRIPYSRDKANTGLGDSYLQNADAVDNNKPADYDADSALMKRTIANN
                     HLNGSDTTHPQINKKKTVNREDSQFFWGWKIFFGLMAFEKADSMRHFRLLPLSWLTWD
                     EASKDKLFVLLPLYVSYYSDAEEYFVLFPFYGYQRDNNAFASMYLINCYWHEYDTAGY
                     DEKTILWPFINWYHSDTKRGFRIFPFYWFSVYTEDGYKVTRSFSLIHYNKEYSNNGLI
                     AKSLYINPLYYKSVNTMDKHDDTFAIPLCLMWQHRDDDGQSLFVAGYYRYRSKSYTRD
                     TMLFLYDYKNYYSSGEAIKTQHSLLLTTMTVTTEPEVLRLRLLWGALFEYDRDKNKPM
                     YDVDALLWLVGVEEDNYSFSHKILPLYYYYSEHQFNLLIIPVLLSGCYRHYYKIVDLG
                     LLGLLYYRNEDLLMNDGRRMVLLGTIFDETKKPERKYHSVGSLWGILWAYEKEEETGF
                     TKFSILKGLYKYTEKDGSGKHTFLWFL"
BASE COUNT          697 a          436 c          356 g          634 t
ORIGIN      
        1 ttttaatggc acctacggca gcatcaatat catcatcatc aatatagggg acatggaacg
       61 ggatgttcat ggaagtatat ccttatgcac aacatcgtat tatttctatt ttcggataaa
      121 agcggcggta agaatatcaa aagattttta ttttcaatga caactatcac aaaaaccaca
      181 ggaatgtatg cttgccagag ccatcttttt ctgtatattt atacaaaccc ttcagaatgg
      241 aaaacttggt aaaacccgtt tcttcttcct tttcatatgc ccataatata ccccacaaac
      301 tgccaactga atggtatttc cgttcaggct tttttgtttc atcaaaaatt gttcccagaa
      361 gaaccatccg cctgccatca ttcattaaca agtcttcatt gcgataatac aacaagccta
      421 aaagccccag atcaacaatc ttataataat gtcggtaaca tccactgagc aaaacaggta
      481 taatcaatag attgaattga tgttcactat agtaataata cagaggaagt atcttatgac
      541 tgaatgaata gttatcctcc tctacaccaa caagccacaa cagagcatca acatcataca
      601 tgggtttgtt tttgtcccgg tcatattcaa acaatgcacc ccagagcagt cgtaagcgta
      661 gtacttcggg ttctgtggtt actgtcatag ttgtcaataa caatgaatgt tgcgtcttaa
      721 tggcttcccc tgaagaataa tagtttttat aatcatataa aaaaagcata gtatcccttg
      781 tatagctctt tgatcggtag cggtaatacc ctgcaacaaa aagtgactga ccatcatcat
      841 cacgatgttg ccacatcaaa cacagcggga tggcaaatgt atcatcatgt ttatccattg
      901 tgttgacgct tttgtagtat aacggattta tataaagact tttagcaatc aagccattgt
      961 tactatattc tttgttgtaa tgaataagtg agaatgacct tgtaacctta taaccatctt
     1021 ctgtatacac actaaaccag taaaaaggaa aaatcctgaa cccgcgctta gtgtctgaat
     1081 gataccaatt gatgaaaggc catagtattg ttttttcatc atagcccgca gtatcatatt
     1141 catgccaata gcaattgata agatacatgc tggcaaaagc attgttatcc ctttgatatc
     1201 cgtaaaaagg aaataaaaca aaatattctt cagcatccga ataatatgaa acatacaatg
     1261 gtagcaaaac aaacagcttg tcttttgaag cttcatccca ggtaagccat gaaagtggaa
     1321 gcaatctaaa atgtcgcatg ctatcagctt tttcaaatgc cattaaacca aaaaatatct
     1381 tccaacccca gaagaactgt gaatcttccc tgttaacggt cttcttttta ttaatctgtg
     1441 gatgcgttgt atcactacca ttcaaatgat tattggcgat agttctcttc atcaaggcac
     1501 tgtcagcatc atagtctgct ggtttattat tatcaacagc atcagcattt tgcaaataac
     1561 tatcgcccaa acctgtattt gctttatccc gtgaatatgg aatacgagat gaaaagctcc
     1621 acattgcata taaccacgat acatcagtat caagatattt tctcccttca gattgggaaa
     1681 gattaacccc aacatctggc ttaaaaatac cagtataggt tttacgcgca taccctgtaa
     1741 ggttaatatg aacagtagtg ttggtatcac tatagttgta aacaaaaggc agtagtatat
     1801 tccctttgct tgtatcatta cgccatgaaa aatagatggg caaaaaatga atatagtatt
     1861 gctcaacacc gtttttttga ggcgcattat ttatggcata gtgcttgcta tggtaataca
     1921 accatatcca tagcctatcc atatccggct gccagcggtg aaaatgaaat aagccaaagg
     1981 aaacaccctc ataagctgtc ctgcgtggac tgcagtataa tggcacatag tagcggtatc
     2041 cattatcagt aaattcgtga aacagcaatg gcacaatcca cagggtgtgt aatgcattat
     2101 ggttgcttct ccaatcaatc aac
//
LOCUS       DAOSZP010000417         2109 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_39866_length_2109_cov_3.986855, whole genome shotgun sequence.
ACCESSION   DAOSZP010000417 DAOSZP010000000
VERSION     DAOSZP010000417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2109)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2109
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39866_length_2109_cov_3.986855"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..856)
                     /locus_tag="PK348_08800"
     CDS             complement(<1..856)
                     /locus_tag="PK348_08800"
                     /inference="COORDINATES: protein motif:HMM:NF014273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HXK66351.1"
                     /translation="MICPESNVVSIPLDAIQCDNNYFKISKPLSRELVRSVLHYGIIE
                     PVLLIKWQEQYVPVCGHNRIIAAQQAGIKDVPAKIVTELSMDDFLQIVRVKLYNNALG
                     TIGKIKSLYLLYDYFNADKKKVMAIAQELSLPASLPEFKNIRSIIELPPVIIQYIDSR
                     SISPKFVMPILRLPQSSIALLEKITSACQLRVNYFNSMVVMIEDIYRMGKDKLLGDSI
                     EFLLTKETILDDDIHNCIYSIRYPQYTEYMVRINEIKKKLNACGIKVDIPAYLEGDSI
                     NVIFEVKKN"
     gene            complement(856..>2109)
                     /locus_tag="PK348_08805"
     CDS             complement(856..>2109)
                     /locus_tag="PK348_08805"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF016362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HXK66352.1"
                     /translation="LSVQKKLAKSYYMRDEIEKAFQTYMDILKNYPADIESLYHIAFI
                     CLGQEEFDIAQRYFDRLVKLKNDNYEILFGAGICYYQNMRIQDAIDNFKLAVQIKPQS
                     EIAQIGAAFACNRKRDYRQALGYISRIADTAQDPDVLYLAKRFQAFMLMRLKKHDEGI
                     KLFQDLLDIARKNANDDAELLALYDLGYAYLKNEQTSLAYEAWNELYRRDRNYKNIQY
                     LVTQLRREMEVDKFSKEESVSVTETITTWMDDAFPQNLLWEMCGLKSTKKYNLKDIVV
                     TTKISYDDGKTVSVSDDVMDRYVALDTENFRIISNRLVSKMGFKVDQILQTYREADGV
                     DFLAINPETKDKVLVWVRRWDKTNVGEITLRNFAQAINDMKVQQGIFITTANLTQAAK
                     ESLKKLPKVTVIYPEEITKLLRGLI"
BASE COUNT          632 a          430 c          309 g          738 t
ORIGIN      
        1 tattcttttt tacctcaaag ataacgttaa tactatcccc ctccaaatat gctggtatat
       61 ccactttaat accacaggcg ttaagttttt tctttatctc attaatacga accatatatt
      121 ctgtatactg tgggtaacga attgaatata tacaattatg gatatcatca tctaatatag
      181 tttcttttgt tagcagaaat tctatactat ctccaagaag tttatccttc cccattctat
      241 aaatatcctc aatcatcact accatgctgt taaaataatt taccctgagc tgacatgcag
      301 aagtaatttt ttccagtaga gctattgagc tttggggtaa ccgtaatatt ggcatgacaa
      361 attttggaga aattgatctg gaatcaatat actgaatgat gaccggaggc aattcaatga
      421 ttgatcttat attcttaaat tcaggtaatg aagcaggcag cgatagttcc tgtgctattg
      481 ccattacctt ttttttatca gcattaaaat aatcatacag taaatataaa ctcttaattt
      541 tcccaatggt ccctaatgca ttgttataca atttaactct tactatctgt aaaaaatcat
      601 ccatgctcaa ttcagtgaca atctttgccg ggacgtcttt tatgcctgct tgctgcgctg
      661 ctataatcct gttatgccca catacaggca cgtattgctc ctgccatttt atgagcagca
      721 caggctcaat aatgccatag tgcaatactg atctgaccag ttccctggac aacggctttg
      781 aaattttgaa ataattatta tcacattgaa ttgcatccaa tggtattgat acaacattac
      841 tttcagggca gatcattata taagccctcg taataatttt gtaatctctt caggataaat
      901 gactgtaact ttggggagtt tttttaagct ttctttagct gcctgagtaa ggttagcagt
      961 tgtaataaat ataccctgtt gcactttcat atcattaatg gcctgtgcaa aatttcgtaa
     1021 tgttatttca cctacatttg ttttatccca ccgcctgacc cacactaaaa ctttatcctt
     1081 tgtttcagga ttaattgcca ggaaatcaac accatctgct tcacgatatg tttgtaatat
     1141 ctgatcaacc ttaaaaccca tttttgatac cagtctgttg gaaataattc tgaaattctc
     1201 agtatctaaa gcaacatacc tgtccataac atcatcagaa acactaactg tttttccatc
     1261 atcataactt attttagtgg taacaacgat atcttttaaa ttatattttt ttgtactttt
     1321 taaaccacac atttcccata ataaattttg tgggaaagca tcatccatcc atgttgtgat
     1381 agtttctgta acggataccg attcttcttt gctgaattta tcaacctcca tttccctgcg
     1441 caattgtgtt acaagatatt gtatattttt atagtttcta tctctgcggt aaagttcatt
     1501 ccatgcttca tatgctaaag aagtctgttc attctttaaa tatgcatacc ccagatcata
     1561 taatgccaga agttctgcat catcatttgc attcttccgt gcaatatcca gtaaatcctg
     1621 aaacaacttt attccttcat catgcttttt caggcgcatc aacataaatg cctgaaatcg
     1681 ttttgccaga tacagtacat cagggtcctg ggctgtatct gcaatcctgg aaatatatcc
     1741 cagagcctgg cgataatcac gtttgcgatt acatgcaaat gcagcaccta tctgagcaat
     1801 ttctgattga ggttttatct gcactgctaa tttaaaatta tcgatagcat cctgtatacg
     1861 catattctgg taataacaga ttcctgctcc aaaaagaatt tcataattgt cattcttaag
     1921 ctttaccagt ctgtcaaaat aacgctgtgc aatatcaaac tcttcctgac caagacagat
     1981 aaacgcaata tggtaaaggg attcaatatc ggcaggataa tttttaagaa tgtccatata
     2041 cgtctggaat gctttttcaa tctcatcccg catataatat gatttagcca atttcttctg
     2101 tactgaaag
//
LOCUS       DAOSZP010000418         2101 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_40064_length_2101_cov_3.204301, whole genome shotgun sequence.
ACCESSION   DAOSZP010000418 DAOSZP010000000
VERSION     DAOSZP010000418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40064_length_2101_cov_3.204301"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..636)
                     /locus_tag="PK348_08810"
     CDS             complement(<1..636)
                     /locus_tag="PK348_08810"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXK66353.1"
                     /translation="MIRMQNVKIVLMIGWRNVFRNKRRSLIVASSIVVGLVVMMFSMA
                     IMNGFNRQMLENTINTSLGHVAIHKKGFFNDMKLSLSFVPKDLCNAIKNIQGVACIGQ
                     RVKFEAMVQSSRSSQGVLVMGIEPEEEKCVSNIFEYAGSDIHSYITDSSNNDILISRS
                     LATKLGIGIGDKCVVMLQDKDGVLAAHAFIVKGLYQSPIESFDKYVVYTGIR"
     gene            complement(652..1863)
                     /locus_tag="PK348_08815"
     CDS             complement(652..1863)
                     /locus_tag="PK348_08815"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXK66354.1"
                     /translation="MLAWRNVWRNKRRTILTLCTIVVGCAMIILLNAFAKGGHDQMIE
                     DATGLVMGHIHIHEKGYWENKTIDYAFEADESIEKALSSNTHIRAYCKRIMCDALIAK
                     GGTTKGVMIQGVEPVREKQVCGLHKCIIKGRYLSDSDSTHAVIGATLAENLKVDVGNT
                     IAIISQGFDGSIAAEHLTINGIFKSGNPEYDKFLLQMPLKQADETFSMMGYIHAYVIK
                     ASHIDNIDAITSSLSKKLPNNVEVLGWEKLMPELVQFIIMDDVSGYLFDLMLFLVVAF
                     GILNTIQMSVYERTRELGIMKAVGTTPGQIVALVILETFFITVIGVLLGVALGAGISY
                     YFTVHPIDYSQYSEEIAVWGITTTIFPATVTIANIVVTVMLTMIVSLLFCIIPARRAS
                     RLHPVEAIRHI"
BASE COUNT          625 a          491 c          370 g          615 t
ORIGIN      
        1 acgtatacct gtatacacca cgtatttatc aaagctttca atgggcgatt ggtatagccc
       61 tttgacaata aatgcatgag ccgcaagcac tccatcttta tcctgcagca tcaccacaca
      121 tttatcgcca atgccaatgc caagctttgt tgcaagtgaa cgtgagataa gtatatcatt
      181 gtttgaactg tccgtaatat atgaatggat atcgctacca gcatattcaa atatatttga
      241 gacacacttt tcttcttcgg gttcaatacc cattaccagc acaccctgcg aactgcggct
      301 tgactgtacc atagcttcaa acttgacacg ctgcccaata cacgccacgc cctgtatatt
      361 tttaattgca ttacacaaat cttttggcac aaatgataat gatagcttca tatcattgaa
      421 aaaacctttt ttatgtatag ccacatgtcc cagtgaagta ttaatggtat tttcaagcat
      481 ctgcctgtta aagccattca tgattgccat ggaaaacatc atcaccacaa gccccaccac
      541 aatggatgaa gcaacaatga gtgaacggcg tttattcctg aatacattgc gccaaccaat
      601 cattaacact atttttacat tttgcatccg tatcatacgt tttttacctg attaaatgtg
      661 ccgaattgcc tcaacaggat gaagccggct tgcacgccgt gctggaataa tacaaaacaa
      721 aagtgacact atcatggtca gcataactgt aaccacaata ttggcgatag ttactgtagc
      781 agggaatatg gttgtggtaa taccccacac cgctatctct tcagaatact gtgagtaatc
      841 aatgggatgc accgtaaaat aataggaaat ccctgcaccc aaggcaacac ccagaagcac
      901 gccaataact gtaatgaaaa atgtttcaag tataaccagt gcaacaatct gacctggcgt
      961 ggtacccact gctttcatga tgccaagctc acgtgtgcgc tcatacactg acatctgaat
     1021 ggtattgaga ataccaaagg ccaccaccag aaacagcata aggtcaaaaa gataaccact
     1081 gacatcatcc atgattataa actgcacaag ctcaggcatt agcttttccc atcccaatac
     1141 ctctacgtta tttggcagtt tttttgaaag tgaagaggta atagcatcaa tgttatctat
     1201 atgtgaagct ttaatgacat aggcgtgaat gtagcccatc atgctgaatg tttcatcagc
     1261 ctgtttcaga ggcatctgca gcaaaaattt atcatattca ggatttccac ttttaaatat
     1321 gccattaatg gtaagatgtt cagctgcaat ggatccatca aacccctgtg aaataatggc
     1381 aatagtattg ccaacgtcaa cctttaagtt ttcggctaat gtggcaccta tcactgcatg
     1441 ggtgctatca ctatcgctca ggtagcggcc tttgataatg catttatgca agccacacac
     1501 ctgcttttca cgcacaggct caacaccctg tatcatgacg ccttttgttg tcccaccttt
     1561 tgcaataagg gcatcacaca tgatacgttt gcagtaagca cgtatatgtg tattactgga
     1621 tagcgccttt tctatggatt catctgcttc aaatgcataa tcaattgttt tattttccca
     1681 gtaccctttt tcatggatat gaatatgccc cataacaagg ccagttgcat cttctatcat
     1741 ctggtcgtgg ccacctttgg caaacgcatt gagcagaata atcatggcac aacccacaac
     1801 gatagtacac agagtaagta ttgtacgccg tttattgcgc cacacattgc gccatgctaa
     1861 catacttatt atttcaaaat cttttttcat ttttattggt ttagaagtta tgttataaat
     1921 ttacagtttc atgatgactt attgtgcaac cgtcattccg ggcttgaccc ggaatctcta
     1981 caccacaacc gtcattccgg gcttgacccg gaatctctac gcttaccata tagatcctga
     2041 atcacgttca ggatgactta ttgaatcacg ttcaggatga cttactgaat taagttcatg
     2101 a
//
LOCUS       DAOSZP010000419         2100 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_40084_length_2100_cov_3.488509, whole genome shotgun sequence.
ACCESSION   DAOSZP010000419 DAOSZP010000000
VERSION     DAOSZP010000419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2100
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40084_length_2100_cov_3.488509"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            301..666
                     /locus_tag="PK348_08820"
     CDS             301..666
                     /locus_tag="PK348_08820"
                     /inference="COORDINATES: protein motif:HMM:NF014672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HXK66355.1"
                     /translation="MSSKQITYIVTHADDDPEMASIPFVLANTAVAMDVTPVIILQGK
                     AVMLAMKGYAEHIHFEGFNPLMDMIKSYIEAGNKLLVCTPCMNKRKIKVEDLIEGAEA
                     IGGAKVNEIVLGSDAVLTY"
     gene            complement(674..1912)
                     /locus_tag="PK348_08825"
     CDS             complement(674..1912)
                     /locus_tag="PK348_08825"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HXK66356.1"
                     /translation="MNTKTRNKASNYAFTVVDTIINNIGARPSCSDDSHVTARVFHKL
                     YKAYCDTSQLQSFITHPAAFLGFLTLVPWIYITSLIFLLLHYYGIAALGFTVANIIAS
                     HQFIFYKGTFDFLYKPKKGYNAIGIINPKKEVRQQIIISGHHDSAYEFRYMRTTPRLY
                     RIRVASIILSMVLSLVIGWLIVVNAYWFQSHTAHIVFMVIIALLGIANMQMLFFKSKN
                     ATPGAGDNLIASAITAVMAKIFSNYRPDYTRLIFVSFDGEESGLKGSKAFAKAYRECI
                     SSMPTYHFNMDSLYKPELIKFLTSDVNGFVKLSNEFAKQCVTIAHSKGHTATLFAMYP
                     GTGATDAAPLAQAGAIATTLIALPTEVEKQDSVYHTMNDTTEHIEPEVVQATIDILYS
                     VIEFLDKEIKGCPQILIKHQ"
BASE COUNT          677 a          331 c          398 g          694 t
ORIGIN      
        1 atttattata tctattgaaa atattcaccc aatgatagtt tttcaacaac tatcgataca
       61 aataattaat gaaggattaa gtagcgttgt ttataaaaat atatttacct attccacttt
      121 tggcaggaat ttctagaata tatataatta tttaattata aaaatagtct cagaaaaaaa
      181 ttaatattat ttataatttt ttgttggcga atattataga attatataaa ttattaaaat
      241 attgtttttt caatatattt attttattta ttacacaata cttattatta aggaggatgt
      301 atgagcagta aacagattac gtatattgta actcatgctg atgatgatcc cgaaatggct
      361 tccattccgt ttgttcttgc aaacacagca gtagcaatgg atgtaacacc ggttatcatt
      421 ttgcagggta aagctgtcat gcttgcaatg aaaggatacg ctgagcacat ccattttgaa
      481 ggattcaatc cactaatgga tatgatcaaa tcatatattg aagcaggcaa taaacttctt
      541 gtttgcacac cgtgcatgaa taaaagaaaa attaaagtag aagatttaat tgaaggtgct
      601 gaagctattg gtggtgcaaa agtaaatgag attgtccttg gaagtgatgc ggtcttgacc
      661 tactaaatga aaattattga tgcttaatta aaatttgtgg acaaccttta atttctttat
      721 caagaaattc aataacactg tatagtatat cgattgttgc ctgtacaact tctggttcaa
      781 tgtgctctgt agtatcattc atggtgtggt acacactatc ctgcttttca acttcagtgg
      841 gaagtgctat gagcgttgtt gcaatggcac cagcttgtgc cagtggtgca gcatcggtgg
      901 cgccggttcc cggatacatg gcaaatagtg tggcagtgtg ccctttgcta tgagctatag
      961 ttacacattg ttttgcaaat tcgtttgaaa gcttgacaaa accattcaca tcgcttgtca
     1021 aaaatttgat gagctctggt ttgtaaaggc tatccatatt aaaatggtat gttggcatgc
     1081 tgctaataca ctcacggtat gcttttgcaa aagcttttga acctttaagt ccactttctt
     1141 caccatcaaa gcttacaaag attaaacggg tatagtctgg gcgatagtta ctgaagattt
     1201 ttgccatcac tgcggtaata gcacttgcta ttaagttatc gccagcacct ggcgtagcat
     1261 ttttgctttt gaaaaaaagc atctgcatgt tggcaatacc aagcagtgca atgattacca
     1321 tgaagacaat gtgtgcagtg tgtgattgaa accagtaagc atttactaca attaaccatc
     1381 caataacgag cgatagtacc atggaaagaa tgattgatgc aacacgtata cggtacaggc
     1441 gcggtgtggt gcgcatataa cggaattcgt atgcactatc gtgatgaccg ctaataatga
     1501 tttgctgacg cacttctttt ttaggattga taatgccaat agcattgtag cctttttttg
     1561 gtttgtataa aaaatcaaag gtgcctttat aaaaaataaa ttgatgcgat gcaatgatat
     1621 ttgcaacagt aaatccaagt gcagctatac catagtagtg cagtaataaa aaaatgagtg
     1681 aagtaatgta tatccatggc acaagcgtta agaaacctaa aaaagcagca ggatgcgtaa
     1741 tgaatgattg aagttgtgaa gtgtcacaat acgctttata cagtttatgg aatacacgtg
     1801 cagttacatg tgaatcatca gagcatgaag gtcgagctcc tatattgttt atgattgtat
     1861 caacaacggt gaatgcatag tttgacgcct tgttacgtgt tttagtattc atgccatttt
     1921 ttccttcatg gattatatat gcaatagtgt tatattgtaa ttgtatctga tacatcccac
     1981 tccattaaca gtaactatcg cccatacttt tattagtagc taactgatgg tgattggtta
     2041 caatagtata ctcttcaaga actttttgta aatgtaaaaa aaatcaggta agaaattttt
//
LOCUS       DAOSZP010000420         2085 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_40409_length_2085_cov_4.476355, whole genome shotgun sequence.
ACCESSION   DAOSZP010000420 DAOSZP010000000
VERSION     DAOSZP010000420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40409_length_2085_cov_4.476355"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(319..786)
                     /locus_tag="PK348_08830"
     CDS             complement(319..786)
                     /locus_tag="PK348_08830"
                     /inference="COORDINATES: protein motif:HMM:NF015020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HXK66357.1"
                     /translation="MYYGVRILITHIIFDMDNTLIDEFGSTVRPCITNFLKTLKKKNL
                     HKYLWTNFTKLRAKEILLLHNVHHYFEKFIYREDYDPLNKGMYKDIRKIGGQLLIDDN
                     PAEIQFVTKLGCKGFLIKPYRKNSKASIADYQAEYDAITRIIHDTKKISSLFL"
     gene            1026..1922
                     /gene="hemC"
                     /locus_tag="PK348_08835"
     CDS             1026..1922
                     /gene="hemC"
                     /locus_tag="PK348_08835"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HXK66358.1"
                     /translation="MIKIGTRGSDLALWQAQFVAGKLACDYEIVTIKTKGDRVHNVSF
                     DKMEGKGFFTKELEDALLEGRIDMAVHSMKDLPTEITSGLTIAAVTQREDSSDILVFR
                     KEAYRVNHWFPLKEGATVGTSSLRRVSQLKCIMPSVDVLPLRGNVPTRVKRLHEGKFD
                     AIILAKAGITRLGILLDEFYTMVLPLSYFLPSPGQGALAIETRYDDAHAINAVIHLHD
                     AATAKAVSAERQFLKQFGAGCHVPLGAFAHCYGSSVIVTGVILSVNGLRSIRHTVEDD
                     DPVKAGERLAQFMIEQGAESFL"
     gene            1919..>2085
                     /locus_tag="PK348_08840"
     CDS             1919..>2085
                     /locus_tag="PK348_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020149503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HXK66359.1"
                     /translation="MTNKHKGFVYLVGAGPGDPELISVKGNELIHTADCIIYDYLANP
                     ALLHNTHAERI"
BASE COUNT          630 a          345 c          425 g          685 t
ORIGIN      
        1 tacaatcatt atttcattat tcataaccat tatctgaata aacatcaatt tcatggtttg
       61 gaaaaaaggg ataaatatac aataaaaatt ttataaaaaa tcagtattta aaaatttaat
      121 gcctttataa tgtttgcgat agttattgct gatatagtca tttgtaataa ttgtaataat
      181 tgtaataaaa aactttctat ataccgtatt tattgaggga ttaattattg cagtattgtt
      241 gtgattttta acataccaat gttgtatgca ttgcataagc aatgattgtt tttttccatt
      301 ggggaggtat tgtttgtttc ataaaaacag actgcttatt ttctttgtgt catgaattat
      361 tcttgttatt gcatcatatt ctgcctgata gtctgctatt gaagcttttg agtttttgcg
      421 atatggtttt ataaggaagc ctttacaacc aagctttgta acaaattgta tttcagcagg
      481 gttatcatct ataagcaact gtcctccaat ctttcttatg tctttataca ttcctttgtt
      541 taagggatca tagtcttcac gataaatgaa cttttcaaaa taatgatgta cattatgcaa
      601 aagtaaaatt tcctttgcgc ggagtttggt aaaatttgtc cacaaatact tatgaagatt
      661 ttttttcttt aaggttttaa gaaaattggt tatacatggc cttactgtag agccaaattc
      721 atcaatcagt gtgttatcca tatcaaatat tatatgtgta atcagtatgc ggactccata
      781 gtacacttac acaacgacta tatgttgact gtacaggttt tatgtataat ggcggagcat
      841 ggtaaaaatg caaataaaaa atattttgcc aaaaaaaaca tggggtcaga gcctccatcg
      901 ttactataaa caggtgattt attacaattt ttatttgaca caatccctgc cgctatcaca
      961 ataatgaaac accagatcat gtttattgtt ggtaaattac ttatactgtg gtacatacta
     1021 ttactatgat aaaaattgga acacggggca gtgatttagc gttatggcag gcgcaatttg
     1081 tggcaggcaa gcttgcatgt gactatgaga tagtaactat caaaacaaag ggtgatcgtg
     1141 tacacaatgt gtcatttgat aaaatggaag gcaaaggttt ttttaccaaa gagttagagg
     1201 atgcactgct tgaaggcagg attgatatgg cggtacactc catgaaggat ctgccaacgg
     1261 agataacatc aggattaacg atagcagcag ttacacaacg cgaggattcc tctgacattc
     1321 ttgttttccg taaagaagcg tatagggtaa atcattggtt tccccttaaa gaaggagcaa
     1381 cggttgggac aagctcgctg cggagggttt cacagctaaa gtgcatcatg ccttcggttg
     1441 atgtgctgcc attacggggc aatgtgccaa cacgggtaaa acgcctgcat gagggcaaat
     1501 ttgatgcaat aatactggca aaagcgggta ttacacggct tggtatattg ctggatgaat
     1561 tttatactat ggtattgccc ttgagttact ttttaccgtc tcccgggcag ggggcacttg
     1621 ctatagagac gcgctatgat gatgcgcatg caatcaatgc ggttatccat cttcatgatg
     1681 ctgctacagc aaaagcagta agcgctgaaa ggcaattttt aaaacagttt ggtgctggat
     1741 gccatgtccc cctgggtgca tttgctcact gttatggttc ttcagttatc gttaccggtg
     1801 ttatactttc tgtaaatgga ttgcggtcta ttcgccatac agttgaggat gatgatcctg
     1861 taaaggctgg tgaacggctg gcacagttta tgatagagca aggagctgag agctttctat
     1921 gacaaataag cataaaggat ttgtttatct ggttggtgcc ggtcctggcg atccagagct
     1981 tatatcagta aaagggaatg aactcatcca tactgctgat tgcattatct atgactatct
     2041 tgcaaatcct gccttattac acaatacaca tgccgaacgt atata
//
LOCUS       DAOSZP010000421         2083 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_40461_length_2083_cov_2.783037, whole genome shotgun sequence.
ACCESSION   DAOSZP010000421 DAOSZP010000000
VERSION     DAOSZP010000421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40461_length_2083_cov_2.783037"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /locus_tag="PK348_08845"
     CDS             <1..573
                     /locus_tag="PK348_08845"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HXK66360.1"
                     /translation="PVEMFVEKPNLETAKKYLREGNYFWNSGIFVWKISVILEYYQKL
                     MPKLYQAFQPLRDLTVEQLTQNDELPWEIKRNVFSQIEAISIDYGIMEKADQRYVIPG
                     EFGWADLGSWASIDDVLSPNADMNRSPYPEKVIFHLSKNCTVFPDTKLIAVVGLHDVV
                     VVDSGNEILVMDKNASQDLREVVNRIKNNH"
     gene            complement(557..1285)
                     /locus_tag="PK348_08850"
     CDS             complement(557..1285)
                     /locus_tag="PK348_08850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66361.1"
                     /translation="MNASNKISLCQPAQGKGCCVCCGLFNCADISFAGLEHFLSDATA
                     EIRYSLVTGNIVRNFELLQKQYTFRETTTYVCPYMGFIDSNTPGCLMHPAVNNGIDRR
                     DCSLFGKEICNDYFCPAYTLLDDRLKKILVRYTDNWYTYSIGIIDPLSFIWIVQLIED
                     KLGEPIVPEKSTPDSIVVTLVNTSLMMVADFFNTVPVPVFYYSEPEYHLHMRELSLNQ
                     SSSLNAGVRDKIKNAISASLNGCS"
     gene            1368..2006
                     /locus_tag="PK348_08855"
     CDS             1368..2006
                     /locus_tag="PK348_08855"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HXK66362.1"
                     /translation="MKYRDNLYELLDVSPLSSKEEIRRAFCTKVKALHPDSTGLSSER
                     FTVELQKVIEAYRILMDDRERAHYDATLLQYPKKLEKHYIPQKRIRYSISLKDLLKKE
                     FLPKKMRRKDIIHEFDHDIEIVCTQQEISKGAVALVQLPSRILCPLCNGQHRDCYVCN
                     GLGRISSASTFEIELPADITKTTILTVDLRKYRPGTFTDLTIKYLRIKVTIV"
BASE COUNT          663 a          432 c          386 g          602 t
ORIGIN      
        1 cccgttgaga tgtttgttga aaaaccaaat ttagaaacag caaaaaaata cctcagggaa
       61 ggtaattact tctggaatag tggtattttt gtatggaaaa tatccgttat tctggaatac
      121 tatcaaaaac ttatgccaaa actataccag gcatttcagc ctctgcgcga tctcaccgta
      181 gaacagctta cccagaatga tgaattgcca tgggaaatca agcgtaatgt attctcacag
      241 attgaagcaa tatccattga ctatggcatc atggaaaaag ctgaccagcg gtatgtaatt
      301 cccggtgaat ttggatgggc tgatttaggt agctgggctt caattgatga tgtactatcg
      361 cccaatgccg atatgaatcg atctccctat cctgaaaagg ttatttttca cctttcaaaa
      421 aattgcaccg tattccctga tactaaactc attgcagtgg ttggcttgca tgacgttgtt
      481 gtggtagatt caggaaatga aatccttgtt atggataaaa atgcatccca ggatctcagg
      541 gaagttgtga atcgtatcaa gaacaaccat taagactggc actaatagcg tttttgattt
      601 tatccctgac tcctgcatta agagaagagc tttgattgag cgatagctct cgcatatgca
      661 ggtggtattc aggctcactg tagtaaaata caggcactgg caccgtatta aaaaaatcag
      721 ctaccatcat caatgatgtg ttaactaaag ttaccacaat actatccgga gtactttttt
      781 caggaactat cggttcccct aacttatctt caatcaattg cacaatccat ataaagctga
      841 gcggatcaat gatgccaatt gaataagtat accagttatc cgtatatcta accagtatct
      901 tttttaagcg atcatcaagt aatgtatatg caggacaaaa gtaatcatta catatttctt
      961 tcccaaatag actgcaatct cttctgtcaa tcccattgtt aactgcaggg tgcatcagac
     1021 atcctggagt attgctgtca ataaacccca tatagggaca cacataggtt gtggtttccc
     1081 tgaacgtata ctgtttttgc agcaattcaa aattccttac aatgttgcca gttaccagtg
     1141 agtagcgtat ttcagcagtt gcgtcagata aaaagtgctc taaccctgca aaagagatat
     1201 cagcacagtt aaaaagccca caacacacac aacacccttt tccttgtgca ggctggcata
     1261 gggatatttt attggaagca ttcatattaa ttcacttgca cattcccatg cattttggta
     1321 tgttaatatt aaacgtacac cacatttata gtatagcaga tttttttatg aaatataggg
     1381 ataatcttta cgagcttctt gatgtttccc cactctcttc aaaggaggag atacggaggg
     1441 ctttctgtac aaaggttaaa gcgttacatc ctgacagcac aggtttaagc tctgagagat
     1501 ttaccgtaga acttcagaag gtcattgaag cttaccgcat cctcatggat gacagagagc
     1561 gtgcccatta tgatgccaca ctcttacaat atcctaaaaa acttgagaag cactatatac
     1621 cacaaaaacg catcagatac tccatatcac ttaaagattt attaaagaag gagtttctac
     1681 caaaaaagat gcgtcgcaag gacattattc atgaatttga ccacgacatt gagatagtat
     1741 gcacacaaca ggaaatttca aaaggggcag tagcattagt tcagttgcca tcccgtatac
     1801 tgtgtcctct ttgtaacggt cagcaccgtg attgttacgt atgcaatggg ttgggacgta
     1861 tatccagtgc ttcaacattc gagattgaac tccctgctga cataacaaaa acaacaatat
     1921 taactgttga cctcagaaaa tacaggccag gtacttttac cgatctaacc ataaagtacc
     1981 tgcgtataaa agtcaccata gtataaaggt agcctacctg aatcgtattc tcaacgaatc
     2041 accatgttta tcatcaaatc cttcaacctg cgacatacgt gat
//
LOCUS       DAOSZP010000422         2080 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_40520_length_2080_cov_3.200988, whole genome shotgun sequence.
ACCESSION   DAOSZP010000422 DAOSZP010000000
VERSION     DAOSZP010000422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2080
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40520_length_2080_cov_3.200988"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            696..>2080
                     /locus_tag="PK348_08860"
     CDS             696..>2080
                     /locus_tag="PK348_08860"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HXK66363.1"
                     /translation="MVKNLSRIIILIMMPALISQCSIMGHYFKSSEKQYSGTIHIEGL
                     KQPVEVRYDNLGIPHIKAHTEEDLFLATGYVMASYRLFQMVMIKMAIQGRLSEFAGKD
                     ALPIDYFMRSLNAKKMVAESMRSIDSHGLMMFTQFTKGVNAYIKGCKELPPEFILAGF
                     TPDEWQPEDGLYIFGMLNLDVSFNFIEELQFLMIAQKLGLEKACHLFPVYKDTELPFE
                     EAKKLRSIITTIPQIEEVSGTLSKLKQSLGLGIPASNNWAIAPTKTNGKSIVANDTHL
                     MASLPSPWIIMHLECPTYHCAGVCLPGIPIVVAGYNGHVAWGETMVMADTQDIFIEKI
                     KEEKGNLYYLYKGEWVPVEKHEEVFTVKGKKHTVTMYSTVHGPLLNMALLSMPFPPKL
                     PVQPPAVDMPYGLALSWVVAGGDKTLNGFYTLGKAKTLAQAREAMKHIQTIYLNIVYG
                     DSNSIGWQVTGL"
BASE COUNT          647 a          358 c          449 g          626 t
ORIGIN      
        1 tgtgggatta tttcaagcta tgaaatgatc tcattgtttg tacggtaaca cttttatcct
       61 ctattttttt aaaatgtata taaactattt gttagtaaag aagggtcgta tttttacatt
      121 tataaatact atagagcttc tggactcctg ttggcaaaat tccctctttg agatgaatat
      181 cctggcttga ccggagtggt taacctcttg ttggccagag gtaatagcat ttagtcagaa
      241 aaattttatc ctacaggtat tatttaattg acatagtagc aaaataatat gaacatccgt
      301 tctatatttg aaccattgta aaaataccaa ttatttaagt gtatgccgat tatgcaggga
      361 agtatgaatc agaataccag cgtttagttc ttgaaatatg aaatggtgac aatctatata
      421 acggatacta gtattgatat tctatttctc aatcagatgg gcatgctggc atagaatgta
      481 gtaagaatta tgaaaatcat gattttttcc catttatact gcaggttgtt ttgtgaatat
      541 tgcaatcatg tacatggcgc gatgtttgta gtagatccat gataaaagct tataaatgtt
      601 ggagaaaagt attgcagaat atgataagca aaatgggtta agaaatatac atttgcttaa
      661 gcctgaaaac tttttttacg ggaggaaatt aaggtatggt aaaaaatctt agccgtatca
      721 tcatactgat catgatgcca gcattgattt cacaatgttc cataatggga cattatttca
      781 aatcatcaga aaaacagtat agcggtacca tacacattga agggttgaaa caaccggtag
      841 aggtgcgcta cgataatcta ggcattcctc acataaaggc gcacaccgaa gaagatttgt
      901 ttttggcaac cggatatgtc atggcatcat accggttatt ccagatggtt atgataaaga
      961 tggctattca gggaaggtta tcagagtttg caggcaagga tgcgctgcct atcgactact
     1021 ttatgcgaag cctcaatgca aaaaaaatgg tagccgaaag catgcggtca attgacagcc
     1081 atggccttat gatgtttaca caatttacaa aaggggtcaa tgcttatatc aagggatgta
     1141 aagagttacc accggaattt atccttgcag gatttacacc tgatgagtgg cagcctgaag
     1201 atggtttgta tatttttggt atgctgaatc tggatgtttc gtttaatttt attgaggagc
     1261 tacagttttt gatgatagca caaaagttag gtctggaaaa agcatgccac cttttccccg
     1321 tttacaaaga tactgaactc ccttttgaag aagcaaaaaa attgcgtagc attataacca
     1381 ctatcccaca aatagaagaa gtttcaggta ccctaagcaa gctaaagcag tccttaggat
     1441 tgggtatacc tgcgtcaaat aactgggcta ttgcgccaac aaaaaccaac ggcaaaagta
     1501 ttgttgcaaa cgatactcat ttaatggcat cgcttccttc accgtggatt attatgcacc
     1561 ttgagtgtcc tacctatcac tgcgctggag tatgtttgcc gggtattccc attgttgttg
     1621 caggctacaa cggtcatgtg gcctgggggg agaccatggt tatggctgat acgcaggaca
     1681 tattcattga aaagataaaa gaagaaaagg gtaacctgta ttacctgtat aaaggtgagt
     1741 gggtgcctgt tgaaaagcat gaggaagtat ttacggtaaa ggggaaaaag catactgtta
     1801 ccatgtacag cacggttcac ggcccgctac ttaatatggc tcttttatca atgccatttc
     1861 caccaaagct tccagtgcag ccgcctgcag ttgatatgcc ctatgggctt gcactatcct
     1921 gggttgtggc aggtggtgat aaaacactca atggatttta tactttaggg aaagctaaga
     1981 cacttgctca ggcacgggaa gcaatgaaac atattcaaac aatctatctt aatattgtat
     2041 atggcgatag taactctatt ggctggcagg taacggggct
//
LOCUS       DAOSZP010000423         2073 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_40703_length_2073_cov_1.820119, whole genome shotgun sequence.
ACCESSION   DAOSZP010000423 DAOSZP010000000
VERSION     DAOSZP010000423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40703_length_2073_cov_1.820119"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..715
                     /locus_tag="PK348_08865"
     CDS             <1..715
                     /locus_tag="PK348_08865"
                     /inference="COORDINATES: protein motif:HMM:NF024216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HXK66364.1"
                     /translation="KWHGIMELLVPITKAYCTDMGFRVTELAIQIYGGYGYCQDYPVE
                     QFMRDLKIASIYEGTNGIQALDLVGRKMSQKKGANFINFLGEINKTLARYKDNPRLAD
                     ISKDVQDAVNLLADMGMFFVQCGKEGKFLIPISNAYPFLNLMGTVTLGWLLFWQSGIA
                     YEKLDEICKQNNVDVNDKKAVAQLAKDHKEAAFYSGKIHSARYYITHVLPHAQAYAKA
                     IKSQNLSMLDIPEESFAIE"
     gene            867..>2073
                     /locus_tag="PK348_08870"
     CDS             867..>2073
                     /locus_tag="PK348_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis decarboxylase"
                     /protein_id="HXK66365.1"
                     /translation="MAYKNLHNFITALEKAGELKRITAEVDPYLEITEIADRMSKTGG
                     PALLFEKVKGSLYPVLINAFGSYKRMQMALHCDSFDDIGQKIYNLITMQPPKTLTEKI
                     KALLMLKDVASLMPKQVSKAPCQKHVVTDGALLDALPILTCWPKDGGPFVTLPIVITK
                     DPETGIQNAGMYRMHKFSNTTTGMHWQYNKDGARHYRKYCQLQQRMPVAVALGGSPAI
                     TYAATAPLPPDIDEMLFAGFLDGEPVELVTAKTVDLLVPAEAEFIIEGYVDPGDETIE
                     GPFGDHTGFYSPADRYPVFHVTCITTRDNPVYPATVVGKPPMEDCYMAKATERIFLPF
                     LKMLIPEIVDIELPLEGVFHNCALVSIKKEYPGQAKKVINALWGLGQMASTKFIAVFD
                     ADIHLRDYST"
BASE COUNT          626 a          393 c          463 g          591 t
ORIGIN      
        1 aaaatggcat ggaattatgg aattgctggt tcccataacc aaggcatatt gtacagatat
       61 gggcttcagg gtaacagagc ttgctattca aatttatggc ggttatgggt attgtcagga
      121 ctatcctgtt gaacagttca tgcgcgattt gaaaattgca tccatatatg aaggcaccaa
      181 tggcatacag gcacttgatt tagttggacg aaagatgtcg cagaagaaag gggcaaactt
      241 cattaatttc cttggtgaga ttaacaaaac tcttgcacgc tacaaggata atccacggct
      301 tgctgatatt tcaaaggatg ttcaggatgc agtcaattta cttgctgaca tgggcatgtt
      361 ctttgtccag tgtggcaagg aaggtaagtt cttaattcca atcagcaatg catatccatt
      421 cttaaatctt atgggaactg ttacattggg ctggcttctt ttctggcaga gtggcattgc
      481 gtatgaaaag cttgacgaaa tttgtaaaca gaataatgtt gatgtaaatg ataaaaaggc
      541 tgttgcgcaa ctggctaaag atcataaaga ggcagcattt tattctggca agattcacag
      601 tgcgcgctac tatattacac atgtactgcc gcatgcgcag gcatatgcaa aagcaataaa
      661 gagtcagaat ctttcaatgc ttgacattcc ggaagaaagc tttgcgatag agtaagcagg
      721 ttgtcacgcg tcacaatcaa cactaaagcg ggctactaac tggcctgctt tttttatagt
      781 aactatcgca catataaaaa atacttgcat taacgttatt tttatgaccg atagttacca
      841 actataaatg tggaaatgag gtttacgtgg catataaaaa tttacacaac tttattactg
      901 ctttagaaaa agctggtgag ctgaaacgta tcacagcaga agtagatcca tatttagaga
      961 ttacggaaat agctgacaga atgagtaaaa ccggtggtcc agcacttctt tttgaaaagg
     1021 taaagggttc tttatacccg gtactcatca atgcgtttgg aagctacaaa cgtatgcaga
     1081 tggcactgca ctgcgattct tttgatgaca ttggtcaaaa aatttataat ctcattacca
     1141 tgcagcctcc taaaacatta accgaaaaaa taaaggcatt actgatgcta aaagatgttg
     1201 catcactgat gcccaaacag gtgagtaaag caccatgcca gaagcatgtg gtaaccgatg
     1261 gcgctttact tgatgctctg ccaattttaa catgctggcc aaaggatggt ggtccgtttg
     1321 ttacattacc cattgtgatt acaaaggatc ctgaaacagg gattcagaat gcaggtatgt
     1381 atcgcatgca caagttttct aacacaacaa ccggtatgca ctggcagtat aacaaagacg
     1441 gtgctcgcca ctatagaaaa tattgtcagt tgcagcaaag gatgccggtt gctgttgctc
     1501 ttggcggaag cccggccatt acatacgctg caacggcacc attgccacct gatattgatg
     1561 aaatgctatt tgctggtttt ttggatggtg aaccagttga acttgttaca gcaaagacag
     1621 tggatttact ggtacctgct gaagccgagt ttattattga gggctatgtt gatcctggtg
     1681 atgaaaccat tgaaggtccc tttggtgatc acacaggatt ttattctcct gctgacagat
     1741 atccagtatt tcatgttacc tgtataacaa cgcgcgataa tcctgtgtat cctgcaacag
     1801 tagtgggtaa acccccaatg gaagactgct atatggcaaa agccactgag cgtatattcc
     1861 tgcctttttt aaagatgctg atacctgaaa ttgttgatat tgaacttccg cttgaaggtg
     1921 tgtttcacaa ctgtgcgctt gtatcaatta aaaaagaata tcccgggcag gcaaagaaag
     1981 ttatcaatgc cctctggggg ttggggcaaa tggcatcgac taagtttatt gcagtgtttg
     2041 atgcagacat acatcttcgt gattacagca cgg
//
LOCUS       DAOSZP010000424         2072 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_40709_length_2072_cov_6.095687, whole genome shotgun sequence.
ACCESSION   DAOSZP010000424 DAOSZP010000000
VERSION     DAOSZP010000424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40709_length_2072_cov_6.095687"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1522)
                     /locus_tag="PK348_08875"
     CDS             complement(<1..1522)
                     /locus_tag="PK348_08875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66366.1"
                     /translation="MEKRLLLYLLSLIVIMLLTIGCKKEKVMEIEPNDTYTQSNLIAI
                     GKPIDGFINTESDIDFYCLEVLNPCLLDINVSAVKGVNSAFTIWKESEGSIVPLKYVD
                     DLRKSAPERFCGIYVENGRYYISISHGDKDQAVKNTENPYTLLILEESADGFEKEPND
                     TILAANPIQIDIPVRGYYSPAFNKANTNAVNPNREEDWFTFYVEDAPVVCDVELSGVA
                     GIEALLDIVDAQGTVLFSSTSQGIGVSQMAKGIGLSVAGNYYIMVAAKNYNANNDVPY
                     SLICTTRNYDNKTEIEPNNIMESATPMVTEIIYGRIYPDNDIDYYQYMGDSSNDVFYR
                     IEINPPMTLDVVSAVYTKKGEEITSINNNGTGDKEVYPNVKLNAPFYVKVWAKRGQVD
                     MDNSYALRIVPVQMGNLVDSEPNDTKEQANNVQGTLINGYISKKHDIDFYVLHYKGRQ
                     KKYFSIKAPDTIPISFSVTDSLGYIIQSITVKAGSSNTLHEIVDMKGYCIVKPLNEM"
     gene            1727..2041
                     /locus_tag="PK348_08880"
     CDS             1727..2041
                     /locus_tag="PK348_08880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66367.1"
                     /translation="MSTKTKLYIFGSTLLIVIVLGMYFYFKYFFTYEQKNITQRKIET
                     ITGQNLTITVFGYDGKIIKRWYGVQKITTPEDSRNYSYFYTRDGKYVQIPGQVWYIAE
                     EE"
BASE COUNT          641 a          396 c          334 g          701 t
ORIGIN      
        1 acatttcatt caatggttta acaatgcagt accctttcat atcaactatc tcatgcaatg
       61 tatttgaact acctgctttc acagtaattg attgtattat atatcccaat gaatcagtaa
      121 cagaaaatga tataggtatg gtatcaggcg ctttaatgga aaaatatttt ttttgacgcc
      181 ctttatagtg taaaacataa aaatcaatat cgtgtttttt tgaaatatat ccatttatca
      241 acgttccctg cacattgtta gcctgttctt ttgtatcatt aggttcacta tcaacaagat
      301 ttcccatttg aacaggaact atccgcaaag cgtagctatt atccatgtca acctgtccgc
      361 gcttagccca tactttaaca taaaaaggtg cattcagctt tacattggga tatacttcct
      421 tatcacctgt cccgttatta tttatagaag ttatttcttc acctttttta gtataaactg
      481 cagatactac atcaagagtc attggcggat ttatctcgat acggtaaaat acatcattgg
      541 aactatcgcc catatactga taatagtcaa tgtcattgtc aggataaatc ctgccataaa
      601 taatttcggt aaccatggga gttgccgatt ccatgatatt gtttggttct atctcggttt
      661 tattatcata atttcgagtt gtacaaataa ggctgtaggg aacatcgttg ttggcattat
      721 aatttttagc tgcaaccata atgtagtaat tccctgctac agataatcca atcccttttg
      781 ccatctgact tacaccaata ccctgcgagg tagacgaaaa aagcacagtg ccctgagcat
      841 caacaatatc aagcagtgct tcaatccccg caacacccga tagctccaca tcacacacta
      901 ctggagcatc ttcgacataa aatgtgaacc aatcctcctc acggtttgga ttaactgcat
      961 tggtatttgc cttattaaaa gcaggtgaat agtagcccct taccgggata tctatctgta
     1021 ttggatttgc ggcaaggatt gtatcatttg gttctttctc aaaaccatca gcagattctt
     1081 caagtatcaa cagtgtgtaa gggttttcgg tattttttac cgcctgatct ttgtcgccat
     1141 gggagattga tatatagtac cttccatttt ccacatatat accgcaaaaa cgctcagggg
     1201 ctgactttcg caaatcatca acatatttta atggcacaat actaccttca ctttccttcc
     1261 agatagtaaa tgcactattc actcctttca cggcactgac gttaatgtct aacaagcatg
     1321 gattcagtac ctcgagacaa taaaaatcaa tatcactttc ggtatttata aatccatcaa
     1381 ttggcttacc aattgcaatc aggtttgatt gggtataggt atcattgggt tctatctcca
     1441 taactttttc ttttttacat ccaatagtca ggagcataat gacaatgagc gatagtagat
     1501 ataataacaa tcttttttcc atattcatct caatttttaa aaaatatttc tcttgtatta
     1561 tatggtttat attgattatg attgttacaa tggttactca taaagtcaag ctacattttt
     1621 taattaactg aaatatatta agatttgaca ttttctttgc tttgctacga tgtatagagt
     1681 aatcgtatgc tattgataag caaatcccat tttgatagag gcttgtatga gcacaaaaac
     1741 taaactatat atttttggta gcaccttgct tattgttata gtacttggga tgtattttta
     1801 ttttaagtat tttttcacct atgagcaaaa aaatattact cagcgaaaga tagaaactat
     1861 tacagggcaa aacttaacca ttacagtatt tggttatgat ggaaaaatca tcaaacgctg
     1921 gtatggagta caaaaaatta caacaccaga ggatagccgg aactattctt acttttatac
     1981 tcgggatgga aagtacgttc agattcccgg acaggtctgg tatattgctg aagaggagta
     2041 accgtgcttg ataaaagcat tcaatatatc aa
//
LOCUS       DAOSZP010000425         2072 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_40716_length_2072_cov_3.509668, whole genome shotgun sequence.
ACCESSION   DAOSZP010000425 DAOSZP010000000
VERSION     DAOSZP010000425.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40716_length_2072_cov_3.509668"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..832)
                     /locus_tag="PK348_08885"
     CDS             complement(<1..832)
                     /locus_tag="PK348_08885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66368.1"
                     /translation="MYGNIVCAALFVLFSFSYALATTFNATLYPTPLVYNHKEFYCQR
                     IIIQADQEFEGQIIASIDGVVTKKAVDSKVKEITIDCYRKTITEPSRLVVTIKNNNAI
                     ITKQTLTIPPVKICKIYCVPFTHVDIGFTQSQKNVRAQNISNIQKELLLLEQTKHYPH
                     YARFKLFTEVSWPVIEFLNSAEIPQQEKSKLIAALHKKDFEVGAFVISHQNRFMSPYA
                     LLASLHNTLRIAQKYSINVKTACIHDVMDFSKITRVLYANNIPYCLIGPNDSRYSVPP
                     L"
     gene            complement(853..1599)
                     /locus_tag="PK348_08890"
     CDS             complement(853..1599)
                     /locus_tag="PK348_08890"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HXK66369.1"
                     /translation="MKQWEKVQRINGDMYMDTIQPVKRNKLTESIVNVLLTNIKNGTL
                     PPGSKLPPERELMHKLGVGRSTLREAVQSLALMGILDVKPGEGTFVRAISKEEIIGPH
                     IFVPIIDAESMADFFEARLLIEPHIAALASKRKNAQDIDAIEETLVQTKRNIGKGDEV
                     DRWAGQFHLQVARASKNIVCVRFLEAILSFLVGRRENAEHNKDFLQWEYESHEKIFRA
                     IKQGDESKAYKTMEQHIQAVLQWYKKLDVV"
     gene            complement(1562..>2072)
                     /locus_tag="PK348_08895"
     CDS             complement(1562..>2072)
                     /locus_tag="PK348_08895"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009279821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HXK66370.1"
                     /translation="LKAVVPEAEKAGVKLAMHPDDPPISPIRGVARIMRSVEHFQKLL
                     DMYPSKVNGIALCQGNFALMTDDLPAVIRTFGKQGKIFFVHFRNVQGTPYKFTETFHD
                     DGMIDMYECMKAYHEVGFDGTMRPDHVPTMEGDSNDNPAYSSIGRLFALGYMKGLLEA
                     VGKSAKDKR"
BASE COUNT          592 a          433 c          370 g          677 t
ORIGIN      
        1 aaaggggcgg cacactatac cgtgaatcgt taggcccaat caaacaatag ggaatattat
       61 ttgcatacaa gaccctggta atctttgaaa agtccatgac atcatgaata catgcggttt
      121 ttacatttat tgaatacttt tgtgcaatgc gcagagtatt gtgcaatgat gcaagcagcg
      181 catacggact catgaatcta ttctgatgcg atataacaaa tgctcccact tcaaaatctt
      241 ttttatgtaa ggcagctata agtttagatt tttcctgctg tggtatttca gcactattca
      301 gaaattcaat aacaggccag ctgacttcag taaaaagctt gaagcgtgca tagtgtgggt
      361 agtgttttgt ttgttcaagg agcaaaagct ctttctgtat atttgaaata ttctgtgcac
      421 gcacattttt ttgcgattgc gtaaacccta tatcgacatg ggtaaatgga acacaataga
      481 ttttgcatat ctttaccggt ggaatagtta gcgtttgttt tgttataatt gcgttattat
      541 tttttatagt aacaactaag cgtgaaggtt cagtgatagt ttttctgtaa caatcgatag
      601 ttatttcttt tactttgcta tcaacagcct tttttgtaac aacaccatca attgatgcaa
      661 tgatctggcc ttcaaattcc tgatcagcct gtatgattat tctctggcaa tagaactctt
      721 tatggttata tactaacggc gttggatata gggtggcatt aaacgtagtt gccaaggcat
      781 aactaaaaga aaataacaca aacaatgctg cacacactat gtttccatac acggcaaacc
      841 tccataaaaa tttcatacaa catctaattt tttataccac tgtaaaacag cctgtatatg
      901 ctgttccata gttttatatg ctttagattc atccccctgt tttattgccc taaaaatttt
      961 ttcatgcgat tcgtattccc attgcaaaaa gtctttgtta tgttcagcat tttcgcgcct
     1021 ccccaccaaa aatgaaagga tagcttcaag aaaacggaca catacaatgt tttttgaagc
     1081 tctggcaacc tgcaagtgaa actggccagc ccagcggtca acttcatccc ctttgccaat
     1141 attccgtttt gtttgtacca gcgtttcttc aatagcatca atatcctgtg cattctttcg
     1201 tttgcttgca agggctgcaa tatggggttc aattaaaagg cgtgcttcaa aaaaatctgc
     1261 catactctca gcatctatta ttggaacaaa aatatgagga ccaataattt cttctttgga
     1321 gatagctctt acaaatgttc cttcgcctgg ttttacatca agaatcccca ttaaggccaa
     1381 tgactgtaca gcctcgcgta aggttgaccg ccctactcct aatttatgca tcaattctcg
     1441 ttcaggtggc aatttgcttc ctggcggaag cgtgccattt tttatattgg tcaacaatac
     1501 attcactatt gattcagtta atttgttacg ctttacaggt tgtatggtat ccatgtacat
     1561 atcaccgttt atcctttgca ctttttccca ctgcttcaag taaacctttc atgtatccta
     1621 aggcaaaaag tctgccaatt gatgaatacg ccgggttatc gttactatcg ccttccattg
     1681 ttggtacgtg atcaggcctc atggttccat caaatccaac ttcgtggtat gctttcatgc
     1741 attcatacat atcaatcatg ccatcatcat ggaatgtttc agtaaattta tatggagttc
     1801 cctgcacatt tctaaagtga acaaaaaaga ttttcccctg cttaccaaat gttctgatga
     1861 ctgctggaag atcatccgtc atgagagcaa aatttccctg acacagtgca atgccattga
     1921 ccttgctggg gtacatatct aaaagctttt gaaaatgttc aaccgaacgc ataatccgtg
     1981 caacaccacg aataggagaa atgggaggat cgtcagggtg catggctaat tttactcctg
     2041 ctttttcagc ctcaggaacc actgctttta aa
//
LOCUS       DAOSZP010000426         2070 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_40770_length_2070_cov_2.976675, whole genome shotgun sequence.
ACCESSION   DAOSZP010000426 DAOSZP010000000
VERSION     DAOSZP010000426.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40770_length_2070_cov_2.976675"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1518)
                     /locus_tag="PK348_08900"
     CDS             complement(<1..1518)
                     /locus_tag="PK348_08900"
                     /inference="COORDINATES: protein motif:HMM:NF039685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6206 family protein"
                     /protein_id="HXK66371.1"
                     /translation="MEDDHKDIIEHLELLNKVYELKRKRIRFMQLASVDTELKELSQN
                     FYKIWNKTSKGFVLKDTVQHSKSIDIIKGLFGQHDEIITMMRGNKAILTVDKIRFYQY
                     LVRHIANTRALHYLYNLFYGEENLSIEKLVKEQLPYLERYLPTFKKFDKTKNLEGLVL
                     SQFDVQNIWSIIEIYDRIRDNLIQDTSLYRQFTKDFTRLYREELKLKILGYGEISTVM
                     QTIHGSTLNQSFIKTKIAESDWVWKRMPPIDTLDAVEALKKVYHEYREILTKKIGIQV
                     PQQQFRYFKSNGWYTVYAGQKKINPQMVGNFLVKRLDEKNAVVLFNKILAELKKWYSF
                     NISDSSLKVGIDGQISNWVLISVDGALNYVGQNDTLVYIDTSTPLYRINGVEQLNAEL
                     FLKNTPWFLRAIIRALFLQEVLDRYYDLRSVVIDCIANLYKEKREDLIPAFIKAANFF
                     FASIDESIQPLTLDEIAKYYKSDAFIWRLFQFARRVDKFVTEVIFRKKYMYRLPEK"
     gene            complement(1529..>2070)
                     /locus_tag="PK348_08905"
     CDS             complement(1529..>2070)
                     /locus_tag="PK348_08905"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HXK66372.1"
                     /translation="TGFGRTGKLWGFEHFDVVPDIVVLGKGMSGGIYPITATVYKEDY
                     AFFFKENPFIHISTFGGSEIGCFAAMAVLDMCDSTLLNNVNEMADYFTKKLSMLSSQY
                     PKASFTVRGLGLMMGLAFNDEMTALFMVKLLFDNGVYVVYSGNDPKVIQFLPPLIITK
                     READLVIAAVQKSLQALSQ"
BASE COUNT          651 a          433 c          311 g          675 t
ORIGIN      
        1 tttttctgga agtcggtaca tatatttctt tcgaaaaata acctcggtta caaatttatc
       61 aactctgcgt gcaaactgga acaggcgcca tataaaagca tcacttttat aatattttgc
      121 aatctcatca agagtcagtg gctgaattga ttcatctatt gaagcaaaga aaaaatttgc
      181 agcttttata aacgcaggta tcaaatcctc acgcttttcc ttatacaggt ttgctataca
      241 gtcaataacc actgaacgca aatcataata cctatccaga acttcctgta aaaataaagc
      301 ccgtataatt gcgcgtaaaa accatggtgt atttttcaaa aacagctctg catttagctg
      361 ttcaaccccg ttaatacgat ataacggtgt actggtatca atataaacaa gagtatcatt
      421 ttgaccaaca taattcaggg caccatcaac cgaaatcagt acccagtttg atatctgccc
      481 atcaataccc actttcaggg aactatcgct tatattaaag ctataccatt tttttaattc
      541 agccagtatc ttattaaaca ggacaactgc atttttctca tccaaacgtt ttaccagaaa
      601 atttccaacc atctgtggat ttattttttt ttgaccagca tatacggtat accatccatt
      661 gcttttaaaa taacgaaact gttgctgtgg cacctgtatg ccaatttttt ttgtaagtat
      721 ttccctgtat tcatgataaa cctttttcag tgcttcaaca gcatcaagag tgtcgatagg
      781 gggcatccgt ttccataccc aatccgattc agcaattttt gttttgataa atgactgatt
      841 cagcgttgat ccatgtatgg tctgcatcac ggtggatatt tcgccatatc ctaatatttt
      901 taatttaagc tcttcgcgat acaaccgtgt aaaatcttta gtaaattgcc ggtataaact
      961 cgtatcctgt ataagattat cacgaatacg gtcatatatt tcaataatag accatatatt
     1021 ctgcacatca aactgcgaca ggactaatcc ttccaaattt tttgtcttat caaatttttt
     1081 gaacgttggg aggtatcgct ctaaataggg caattgttcc ttaacaagtt tttcaattga
     1141 aaggttttct tcaccataaa aaagattata cagatagtga agtgcacgcg tgtttgcaat
     1201 atggcgtacc aaatactgat aaaagcgtat cttatcaact gtaagtatag ctttattacc
     1261 ccgcatcatc gttataattt catcatgttg tccaaaaagt cctttaataa tatcaatact
     1321 ttttgaatgc tgcacagtat cttttaacac aaatccctta gatgttttat tccagatctt
     1381 ataaaaattt tgtgacagtt ccttaagttc agtatcaaca gatgctaatt gcataaaccg
     1441 tatccgcttt cgtttcagtt catacacttt gtttaatagt tctaaatgct ctatgatatc
     1501 tttatgatca tcttccataa aagcctcctc actgtgataa ggcttgtagt gacttctgta
     1561 ctgctgcaat aactaaatct gcttcccgct tagtaataat aagtggaggc aaaaactgaa
     1621 ttactttagg atcattccct gaatacacaa catatactcc gttatcaaat agaagcttaa
     1681 ccataaaaag cgctgtcatc tcatcgttaa atgcaagacc catcatgagg ccaagtcctc
     1741 taaccgtaaa tgaagctttt ggatactgtg aggaaagcat acttagtttt tttgtaaaat
     1801 aatctgccat ttcattcaca ttgtttagca atgtgctatc gcacatatca agaaccgcca
     1861 tagctgcaaa gcagccaatt tcactgcctc caaacgttga gatatgtata aaaggatttt
     1921 ctttgaaaaa gaacgcatag tcttctttat atactgttgc agtaattgga taaatgccac
     1981 cactcatgcc tttgcccaga accacaatgt caggcaccac atcaaaatgt tcaaagcccc
     2041 ataacttccc cgtgcgacca aagccagttt
//
LOCUS       DAOSZP010000427         2067 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_40849_length_2067_cov_2.990557, whole genome shotgun sequence.
ACCESSION   DAOSZP010000427 DAOSZP010000000
VERSION     DAOSZP010000427.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2067
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40849_length_2067_cov_2.990557"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /locus_tag="PK348_08910"
     CDS             complement(<1..327)
                     /locus_tag="PK348_08910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66373.1"
                     /translation="MFQVYFTADLDEELSNKLEEHAQSISILYDKKAVIALFNSEEEI
                     PACARDYILSIQTLDEEIWKYRYLDSFNGIAVTPSVFIYPATKGKMCDSSYKYTIAIN
                     PKDAFGD"
     gene            complement(334..>1763)
                     /locus_tag="PK348_08915"
     CDS             complement(334..>1763)
                     /locus_tag="PK348_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TSCPD domain-containing protein"
                     /protein_id="HXK66374.1"
                     /translation="GEQPLLPYESCNLGSINLARCVVHNGGAAAIDYELLKDITYKAV
                     HFLDNVIDVNKYPLPMIEENTKANRKIGLGVMGFADMLIALGVPYNSEAALSIAKNVM
                     ATIQEASKEASRALAKERGAFPNFPLSEYAEKGQEPLRNATTTTIAPTGTISIIAGTS
                     SGVEPIFALAYVRNVLDNNKLVESNPLFVEYCKQKGIYSEELMQKVAKTGSVAHINEV
                     PDDIKAIFVTAHDITPQWHVRIQAAFQEFVDNAVSKTVNFPNSATVQDIETVYKLAFE
                     LGCKGITVYRDGSRESQVLQVEKEKPLERPVLKIQPRPRKEVTWGKTLKMNTGCGSLY
                     VTINEDEHGLFEVFATMGKAGGCAASQTEAVSRLISLSLRSGIEPQQIIKQLKGVRCP
                     NQAWVKGGKIYSCADAIAKAMERYINPDADQSETIDDMYKNIAETNGKGSDTVMVGVC
                     PECHGPLEFESGCSVCRMCGFSRCG"
     assembly_gap    1764..1773
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1774..>2067)
                     /locus_tag="PK348_08920"
     CDS             complement(<1774..>2067)
                     /locus_tag="PK348_08920"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276855.1"
                     /note="Catalyzes the rate-limiting step in dNTP synthesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     alpha"
                     /protein_id="HXK66375.1"
                     /translation="HPDIIEFIRAKEKNDALTNFNCSVAVSDAFMKAVENDEEYDLIN
                     PRSGKSAGKLKAREVFELIVQMAWKNGEPGIVFIDRINEKNVLKKIGLIESTN"
BASE COUNT          554 a          478 c          384 g          641 t
ORIGIN      
        1 atcaccaaag gcatcctttg gattaatggc aatggtgtat ttgtatgagc tatcgcacat
       61 ctttcctttt gttgcaggat aaataaatac agaaggggtt actgctatac cattgaacga
      121 atcaagatag cggtatttcc agatttcttc atcaagtgtt tgtatggaaa gaatataatc
      181 gcgagcacat gcagggattt cttcctcaga attgaacagg gcaattactg cttttttatc
      241 atataatatg gatatactct gtgcatgctc ttcaagcttg ttgctcaatt cttcatcaag
      301 gtcagcagta aaatacacct gaaacatgta ccattacccg caacgtgaaa aaccacacat
      361 acgacatact gaacagcccg attcaaattc cagtgggccg tggcattcag ggcacacacc
      421 caccatgacc gtatcacttc ctttgccgtt tgtttctgct atatttttat acatgtcgtc
      481 aatggtttca ctttgatcag catcaggatt tatatagcgc tccatggctt ttgctattgc
      541 atcagcgcag gaataaatct tgccgccctt aacccatgcc tggtttggac agcgcacacc
      601 tttaagctgc ttgataatct gctgtggttc aatcccggaa cgcagcgaca gcgagattaa
      661 cctgctcact gcctccgtct gacttgctgc acaaccaccg gctttgccca tggtggcaaa
      721 tacctcaaac aagccatgct catcttcgtt gatagtgaca tacaatgatc cacagccagt
      781 attcatcttt aatgttttgc cccaggtaac ttccttacgt gggcgcggct gtattttcaa
      841 taccgggcgt tccagaggct tttctttttc aacctgcaat acctgtgatt ctcgtgatcc
      901 atcacggtat actgttatgc ccttacatcc cagttcaaac gccagtttat atacggtttc
      961 aatatcctgt accgttgcag aattggggaa attaacagtt ttagaaacag cattatcaac
     1021 aaactcttga aaagcagcct gaatgcgtac atgccactga ggagtaatat catgtgcggt
     1081 aacaaagatt gcttttatat catcggggac ttcgttgatg tgagctactg aacctgtttt
     1141 tgcaaccttt tgcatcagtt cttcgctata tatccctttt tgtttacaat attccacaaa
     1201 cagcggattt gactcaacaa gcttattgtt atcaagcaca ttgcggacat atgcaagggc
     1261 aaatattggt tcaacaccac ttgatgtacc ggctataata cttatggtgc ccgttggagc
     1321 tattgtggtg gtagtagcat tgcgaagagg ttcctgccct ttttcagcat actctgaaag
     1381 cggaaagttt gggaatgcac cacgctcctt tgccagagca cgtgaagcct ctttagaagc
     1441 ttcctgaatg gttgccataa cattctttgc aatgctcagt gccgcctcag aattatatgg
     1501 tacaccaagc gctatgagca tgtcggcaaa acccataacg ccaagtccaa ttttacggtt
     1561 ggcttttgta ttttcttcaa tcatgggaag agggtactta tttacatcaa ttacattatc
     1621 aagaaaatgc actgccttat aggttatatc tttcaataat tcataatcaa tagctgcagc
     1681 accaccatta tgcaccacac atcgggccag gttaattgaa cctaaattgc atgattcata
     1741 cggtaatagt ggttgctcac cacnnnnnnn nnngattggt gctttcaata agaccaattt
     1801 tctttaatac attcttttca tttattctgt caataaacac tatacccggc tcaccatttt
     1861 tccatgccat ttgcacaata agttcaaaca cttcacgtgc ctttagcttt cctgctgact
     1921 ttccgcttcg cggattaata agatcatatt cttcatcatt ttcaactgct ttcatgaagg
     1981 catcacttac tgccaccgag cagttaaaat tggtaagtgc atcatttttt tccttggcac
     2041 ggataaattc tatgatatca ggatgtg
//
LOCUS       DAOSZP010000428         2066 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_40864_length_2066_cov_4.904028, whole genome shotgun sequence.
ACCESSION   DAOSZP010000428 DAOSZP010000000
VERSION     DAOSZP010000428.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40864_length_2066_cov_4.904028"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            157..2040
                     /locus_tag="PK348_08925"
     CDS             157..2040
                     /locus_tag="PK348_08925"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HXK66376.1"
                     /translation="MISVSMRNQLADQRDVHFALFELLKLDELKKIPRFADHDKDIYE
                     ATIDLAFRMAVEEVYPVNREADKIGVKYNPEAKKVIIPDLLKAPLKKYNEAGFVGIME
                     EAEIGGMGMPFTIAIACNEIFTAASLAFTSYPSLAHGAAALIKNFGSEEQKQMYLEKM
                     LTGQWGGTMCLTEPEAGSDVGNLKTKAIPQPDGTYLIQGQKIFITAGDNDFYENIIHP
                     VLARIEGDPPGTKGISIFIVPKYRINPDGSLGEHNDVICSGIEHKMGIHGSATCTLNF
                     GDNGKCVGWLLGQPRQGMKIMFHMMNEARLDVALQGLSLSSAAYMHAVTYARNRIQGV
                     HPTQMMNPEAPKVAIIEHPDVKRMLLWMKGYVEGMRMLVYYLAQNVTLSEVLDGDAKK
                     EAQGMVEILIPIAKAGCTDTAVLVTSEAIQVYGGYGYTSDYPVEQYMRDAKITQIYEG
                     TNGIQSMDLTMRKILMNPEQYNYNIMKKRMAETIAKAKGIVEDKYIELVEKGIQKLDE
                     VITMLKDQMAAGRFLHIFAACTPLQQAMFMLTLAWLHLWSLTLTIPKMKELVGDKKGE
                     ERDKFLADNEEAAYYSGRVLSSQFYLGAEFPKFFGRIDALLFNETAVIKASKDIFTGA
                     LLE"
BASE COUNT          650 a          382 c          454 g          580 t
ORIGIN      
        1 atttttaaat aatatcatta ttattgaata tacaaaattt tacttacaaa taatatatac
       61 attgacaaaa tagtatgaca tgtatatata tgttattaat aatgttatac ttttattttt
      121 caatcatttt aacaatacta atagtggggg tatagtatga tttctgtatc aatgcgtaat
      181 caactggcag atcaaaggga tgtacatttt gcactctttg aattactaaa actggatgag
      241 ttaaaaaaaa taccacggtt tgctgatcat gataaggata tatatgaggc aaccatcgat
      301 ttggcattca ggatggcagt agaagaagta tatcctgtta acagggaagc agacaaaatt
      361 ggtgttaaat acaaccctga ggcaaaaaaa gtaattattc ctgatttact caaagcccca
      421 ttgaaaaaat ataatgaagc cggatttgtc ggcataatgg aagaggctga aattggtggt
      481 atgggtatgc cttttaccat agcaatagcg tgtaatgaga ttttcactgc agcaagcctt
      541 gcatttacat catatccttc ccttgctcat ggtgcagcag cactcatcaa aaattttggt
      601 tctgaagaac agaaacaaat gtatctggaa aaaatgctca ccggacaatg gggtggcacc
      661 atgtgcctta ccgaacctga agctggctct gatgtgggca atctgaaaac aaaagctatc
      721 cctcagcctg atggtaccta tctcattcag ggacagaaaa tttttattac tgcaggtgat
      781 aacgactttt atgaaaatat tattcatccg gttcttgccc gcattgaagg cgatccacct
      841 ggcacaaaag gtatttcaat atttatagta ccaaaataca gaataaatcc tgatggcagt
      901 ttaggtgagc ataatgatgt catatgttcg ggtattgagc acaagatggg tatccatggt
      961 tctgctacct gtacattgaa ctttggtgat aatggcaaat gtgttgggtg gttgttaggg
     1021 caaccacgac agggtatgaa gatcatgttc cacatgatga atgaagctag acttgatgtt
     1081 gccttgcagg ggctttcgtt gtcatctgct gcctacatgc atgcagtcac atacgcacgc
     1141 aaccgtatac agggagtaca tcctactcag atgatgaacc ctgaagcccc caaggtagct
     1201 attattgaac atcctgatgt taaacgcatg cttttgtgga tgaaaggcta tgtggaaggc
     1261 atgcgtatgc ttgtgtatta tctagctcaa aacgtaacat tatccgaagt actagacggc
     1321 gatgcaaaga aggaagccca gggtatggtg gagatactta tccccattgc aaaagctggc
     1381 tgtactgaca ctgctgtgct ggttacctca gaagctatac aggtctatgg tggatatgga
     1441 tatacttctg attaccccgt tgagcagtac atgcgtgatg ccaagataac ccagatttac
     1501 gagggtacca acggcatcca gtccatggac ctaaccatgc gtaaaattct gatgaatcct
     1561 gagcagtaca actacaatat catgaagaaa cgaatggctg aaactattgc caaagccaaa
     1621 ggcatagttg aagataaata cattgaactg gttgaaaagg gaatccagaa gttagatgaa
     1681 gtcatcacca tgttgaaaga ccagatggct gcaggaagat ttttacacat ctttgctgcc
     1741 tgtacaccac tgcagcaggc catgttcatg ctgacgcttg catggttaca tctgtggtca
     1801 ctgacattga ccataccaaa gatgaaagag cttgttggtg acaaaaaagg tgaagagcgt
     1861 gacaaattcc ttgctgacaa tgaagaagca gcatactatt ccgggcgtgt gttatcatca
     1921 cagttttacc tgggtgctga atttccaaaa ttctttggcc gaattgatgc gctgctgttt
     1981 aatgaaactg cggttatcaa ggcaagcaaa gacatcttta ccggtgcctt gttagagtaa
     2041 gatgatacat tgtgcaggcg agcaaa
//
LOCUS       DAOSZP010000429         2062 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_40971_length_2062_cov_3.244145, whole genome shotgun sequence.
ACCESSION   DAOSZP010000429 DAOSZP010000000
VERSION     DAOSZP010000429.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40971_length_2062_cov_3.244145"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..637)
                     /locus_tag="PK348_08930"
     CDS             complement(<1..637)
                     /locus_tag="PK348_08930"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HXK66377.1"
                     /translation="MKSPYASKPWLKHYDPGVPATIQYHQTPLHKMLENSVNEFPHNT
                     ALIFEGYRVSYAELFIMVKKFASFLDSIGVKKGDVVAILLPNMIQTVVAYYATISIGG
                     IVAMCNPLYSDRELEYQFNDSGAKVLVCLDLLANRMIKLRSVTSIKHIIYASIGDYLP
                     FPKNILFPLVARKNNMKADVYPAPDVYHFTYCLKVSKPNPPQVNITFDDVVA"
     gene            841..972
                     /locus_tag="PK348_08935"
     CDS             841..972
                     /locus_tag="PK348_08935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66378.1"
                     /translation="MPVIETSIERKEAEMLVKKATVVCAYKDIIFQANDEYYELSPK"
     gene            complement(998..>2062)
                     /locus_tag="PK348_08940"
     CDS             complement(998..>2062)
                     /locus_tag="PK348_08940"
                     /inference="COORDINATES: protein motif:HMM:NF012239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HXK66379.1"
                     /translation="SMYVSEEANPNNTLHVEGVELYYDAEILKKGVVLIDTPGIGSTH
                     RHNTEATLNFLPQCDAALFLMSADPPITEVELEFLKQVATRVNKIFFILNKIDYLSPE
                     ELEEVQRFIMRVIREKAGIQEDIALYPVSAKLGLEAKCSHDTTLLSQSRLDYLEDHII
                     RFMSQKKEKILQASIPRKSFDILTDLEMQLRLSLKAYTMPQEELQQKLMLFDETIQQA
                     EQQKTFSGDILAGERKRLLELLEEQSENLRQKGRHYLESVANSCLYEKNTIDENAITQ
                     ALALAIPGFFEHELDQMSRFFDTHVITITRKAIDIAIQKKQTYASEVAGQVTHLEQLI
                     AALEGHKRHLASFIRDSETI"
BASE COUNT          610 a          381 c          403 g          668 t
ORIGIN      
        1 aggctacgac atcatcaaag gtaatgttta cctgcggagg atttggcttt gacaccttga
       61 ggcaatatgt aaagtggtac acatcaggtg cggggtacac atctgctttc atattatttt
      121 tacgtgcaac aagaggaaaa agaatatttt tagggaatgg gagatagtca ccaatagaag
      181 catatatgat gtgcttaatg cttgtgacag atctgagctt aatcattcta tttgctaaca
      241 gatcaaggca caccagaacc tttgcaccag aatcgttaaa ctgatactca agctccctat
      301 cgctgtacaa tgggttacac attgcaacaa taccccctat gcttatggtg gcgtagtaag
      361 ctaccacggt ctggatcata ttgggaagca gaattgcaac aacatcgcct tttttgacgc
      421 caattgaatc aagaaatgag gcaaattttt ttaccatgat aaagagttcg gcatagctta
      481 ctctgtagcc ttcaaagata agtgcagtgt tgtgcgggaa ttcattaaca ctgttttcaa
      541 gcattttgtg aagcggggtt tgatggtact gtattgtagc tggcacacct ggatcatagt
      601 gttttaacca tggttttgat gcatagggtg atttcatatt ttaatctcct attttgtata
      661 aacattatat tgtattatat ttatattaat ttgtatttgt caataaaaaa tttatattta
      721 tttttgctat ttttatgata aggaatagta ttaaaaaaac aattaatcca tatcttctta
      781 aaataaatac tggatttttt ttacaacagg aactcaacgg gtataatagt ttaccataag
      841 gtgccagtaa ttgaaactag catcgagcgt aaagaagcag aaatgctggt aaaaaaagct
      901 acagtagtat gcgcatacaa ggatataata tttcaggcaa atgatgaata ctatgaacta
      961 tcgcccaaat aataaaccat tacagtatgc atttgtatta tattgtttca gaatccctga
     1021 taaacgatgc caagtgcctt ttatgtcctt ctagtgcagc aatcagttgt tctagatgtg
     1081 taacctgccc ggcaacttca ctggcatatg tttgtttctt ctggatagcg atatcgattg
     1141 cttttcgggt aatggtaata acatgagtat caaaaaagcg cgacatctgg tcaagctcat
     1201 gttcgaaaaa tcccggtata gccagagcaa gtgcttgtgt tatggcattt tcatctattg
     1261 tatttttttc gtaaagacaa ctattagcca ccgattcaag ataatgccgt cctttttgtc
     1321 gtaaattttc cgactgttct tctaaaagtt ccagcaaccg tttgcgctca cctgctaata
     1381 tatcaccgga aaacgttttc tgttgctctg cctgttgtat ggtttcatca aacagcatga
     1441 gtttttgttg cagttcttcc tgtggcatag tgtatgcttt taaagaaagc ctgagctgca
     1501 tctccaggtc agtcaggata tcaaaacttt tgcgtggtat actggcctgc agtatttttt
     1561 ccttcttttg tgacatgaag cgtatgatat ggtcttccag ataatcaagc ctgctttgtg
     1621 aaagcagtgt tgtatcatgt gaacatttgg cctcaagtcc cagctttgcg gataccggat
     1681 acagtgcaat atcttcctgt ataccggctt tctcacgaat gacacgcatt ataaatcgct
     1741 gaacctcttc aagctcttct ggactaagat aatcaatctt attgagtata aaaaatattt
     1801 tattaaccct ggtagcaacc tgctttaaaa attccagttc aacctcggta ataggcggat
     1861 cagccgacat taaaaaaagt gccgcatcac actggggaag aaagttgaga gttgcttcgg
     1921 tattgtggcg atgagttgaa ccaataccgg gagtatcaat aagcactact ccctttttaa
     1981 gtatttcggc atcataatat aattcaactc cttcaacgtg gagtgtatta tttgggttgg
     2041 cctcttctga aacatacata ga
//
LOCUS       DAOSZP010000430         2060 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_41012_length_2060_cov_3.168080, whole genome shotgun sequence.
ACCESSION   DAOSZP010000430 DAOSZP010000000
VERSION     DAOSZP010000430.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2060
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41012_length_2060_cov_3.168080"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            271..1833
                     /locus_tag="PK348_08945"
     CDS             271..1833
                     /locus_tag="PK348_08945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66380.1"
                     /translation="MKKFFTYSIAIIAGIFVLYLIGYICIPRLLVIGKETKHKIYVSL
                     GFHANFYHSYRIDTNDEAGFGKDIRIVRKIIEVLDEKNKKGIPVKGVWDFENLFTLEE
                     ILPKYAPDIIQNIKRRVKKHGDEVILMSYNNGLVSAMTRDEFVYSMQNAITNTRKSGV
                     KDIFGIFLPYVRPQEMMTSAGNFNLYKELGIQGVVLYYSAIPFDAFRVFVDELSLEEA
                     HNPLIYNNTITNERMLVIPAYNHADLIENVSIYNWVQMLRHEQLRGNIKNDVLICINS
                     DADDSYWYGYNLPSYLKWVPNTGGLSQLIDDIASLDYVEFTTIHDYVQHHKPVKEISF
                     GQDTADGSFNGYVSWAEKKYCSDYWTAVEKDRMAHAFVKALYKYIKQPISEKIQAALV
                     ESFQKRMRLLSTTNFGMATPFLAKNREHVVDDLIKQMQSITDTVIRDLKAYAQKICSQ
                     DKKAQRFSNYEYVNSVLLLKQNADNFVFTISGELPVGYDYFLKYNDTPIPVFVFSQNG
                     ISRFFCKGRDSF"
     gene            1838..>2060
                     /locus_tag="PK348_08950"
     CDS             1838..>2060
                     /locus_tag="PK348_08950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66381.1"
                     /translation="MYHIVRKPHSQTQYTTHATQQLLKNSTIVIKFKHGVNSIEVNGK
                     EVLDEYSFLPWIQYNGKNYRPKTVNIKIIN"
BASE COUNT          686 a          336 c          388 g          650 t
ORIGIN      
        1 acacctttga tttttactgt gaaaatggta tccattgcct aaaattctac tggggaccac
       61 caccagaagc cttgtatcat gctatctaag tgctacagac ccatcatcaa taagttgttg
      121 cattcaccag ctttatctgg tatactacgg atattgttta ttttataata gctatgagga
      181 attattattc tgggtcacga tcacacagtg gttgggataa taatttagca ttatgattac
      241 tacaacttgc tttcagtggg agatataaat atgaaaaagt tttttacata ttcaattgca
      301 atcattgcag gcatatttgt actctattta attggttata tttgtattcc acgcctttta
      361 gtaattggca aagagacaaa gcataaaata tatgtgtcat tgggatttca tgccaatttt
      421 tatcattcat accgaataga taccaacgat gaagcaggtt ttggcaaaga tatacgcatt
      481 gtacgtaaga ttattgaagt gctggatgaa aagaataaaa aaggtattcc tgttaaaggt
      541 gtgtgggatt ttgaaaatct atttaccctg gaagagatat taccaaagta tgctcccgat
      601 atcatacaga acatcaaacg tcgtgtgaag aagcatggtg atgaagtcat tttgatgtcg
      661 tataataatg gacttgtttc tgcaatgact cgagatgaat ttgtgtattc aatgcaaaac
      721 gctattacca atacaaggaa aagtggagtg aaagatattt ttggaatttt tttgccctat
      781 gttcgtccac aggaaatgat gacatctgca gggaatttta atctatacaa ggagcttggt
      841 atacagggag ttgttctata ttacagtgct attccgtttg atgcatttag agtttttgtt
      901 gatgaattat cgcttgaaga agctcacaat ccgcttatat acaataatac aataacaaat
      961 gaaagaatgc ttgtaatacc tgcatataat catgccgacc tcattgaaaa tgtaagcatt
     1021 tataactggg tacagatgct tcgccatgaa caattacgtg ggaatataaa aaatgatgta
     1081 ttgatatgca taaatagtga tgctgatgat tcatactggt atgggtataa tctaccttca
     1141 tatttaaaat gggtgcctaa tactggcggg ctatcacaac tcattgatga tatcgcatca
     1201 cttgattacg ttgagtttac tacaatccat gattacgtgc aacatcataa gcctgtaaaa
     1261 gaaatttcat ttgggcagga tactgctgat ggcagtttta atggttatgt atcatgggct
     1321 gaaaaaaaat actgcagcga ttactggact gcagttgaaa aagacagaat ggctcatgca
     1381 tttgtaaaag cgctgtataa atacatcaag caaccgattt ctgaaaagat acaagctgca
     1441 ttggttgaat cgtttcaaaa acgtatgcgg cttctttcta caacaaactt tggtatggca
     1501 acaccatttc tggcaaaaaa ccgtgaacac gttgttgatg atctcattaa acaaatgcaa
     1561 tcaataaccg atactgttat acgtgattta aaagcatatg cacaaaagat ttgcagccag
     1621 gataaaaaag cacagagatt cagtaactac gaatatgtta attctgtgtt acttcttaaa
     1681 caaaatgctg ataattttgt gttcacaatt tcgggtgaat tgcctgtagg ttacgactat
     1741 ttcctgaaat acaatgatac gccaatacct gtgtttgttt tttcacagaa tggaataagc
     1801 cgtttttttt gtaaagggag ggattccttc tgatggtgtg tatcacattg ttcgcaaacc
     1861 tcacagccaa actcagtaca caacacatgc tactcaacag cttctcaaaa acagtacaat
     1921 tgttattaaa ttcaagcatg gagttaattc aatagaagtt aatgggaaag aggttcttga
     1981 tgaatatagt tttcttccct ggatacagta caatggcaaa aactatcgcc ccaaaacagt
     2041 aaacataaag ataattaatg
//
LOCUS       DAOSZP010000431         2057 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_41075_length_2057_cov_3.532468, whole genome shotgun sequence.
ACCESSION   DAOSZP010000431 DAOSZP010000000
VERSION     DAOSZP010000431.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2057
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41075_length_2057_cov_3.532468"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="PK348_08955"
     CDS             <1..209
                     /locus_tag="PK348_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015948150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HXK66382.1"
                     /translation="ESSGNVQEVKEIRIDCDNDCVLLKINQIGDAACHTGYRSCFYRV
                     VEGGDLKVDGIKIFNPEDKYGDKK"
     gene            206..1102
                     /gene="hisG"
                     /locus_tag="PK348_08960"
     CDS             206..1102
                     /gene="hisG"
                     /locus_tag="PK348_08960"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012749648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HXK66383.1"
                     /translation="MSLIKLGIPKGSLESATIELFKRAGFSISVSSRNYFPSIDDPEI
                     SCSLVRAQEMSKYVESGVLDVGLTGLDWILENESDVEIISDLIYSKVSTQKAKWVLAV
                     PNDSPIKTLEDCAGKTISTELVNFTKKYFADRNIPVKVEFSWGATEAKVVEGLVDAIV
                     EVTETGSTIRAHGLKIIYTLLETNTKLIANKQSMKDSAKRAKIKQIALLLRGALNANN
                     LVGIKMNVPEDKLADVVKLIPSLTSPTVSKLYNATWFSVESVIQESVVRDLIPKLINA
                     GADGIIEYPLNKVVSQDDVCLK"
     gene            1121..1852
                     /locus_tag="PK348_08965"
     CDS             1121..1852
                     /locus_tag="PK348_08965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66384.1"
                     /translation="MQHDVISRGRQYIKSFAVTLPAPRFYADSYWYLAIAKKISTVSS
                     VIAYCAGCMRQKGSALGHSYYHAEKVAIESAAIVLKEKGISSQSIHLAMLALIAGFLH
                     DYYREKKDHPAKAAEYVQAHLSHFMPESDIDAISFAIRNHEAFKEYQMVDNSDIMLLS
                     NALYDADKFRWGPDNFIYTIWDMIATMNIPIQDIIAHFPGGVAHINSIRYTFRSKTGM
                     DYGPEFIDQGMLIAEKLLQFLQNNS"
BASE COUNT          656 a          380 c          451 g          570 t
ORIGIN      
        1 gcgagtcttc tggcaacgtg caggaagtaa aagaaatacg cattgattgt gataacgatt
       61 gtgtgttact aaaaattaat cagataggcg atgcagcatg ccataccggt tatcgtagct
      121 gcttttacag ggttgttgaa ggcggtgatc taaaagtaga tggtataaaa atctttaatc
      181 ctgaagataa atatggagat aaaaaatgag ccttatcaag ttaggaattc ctaaaggtag
      241 tttagaaagt gcaacaatag aactttttaa gcgggcaggt ttttcaatat cagtatcatc
      301 acggaattac ttccccagca ttgatgaccc cgagattagc tgttcccttg tccgtgcaca
      361 ggagatgtca aaatatgtgg aaagcggcgt acttgatgtt gggctaaccg gccttgactg
      421 gatactggaa aacgagtctg atgttgaaat aatatctgac ctgatttatt ccaaggttag
      481 cactcagaag gcaaaatggg ttttagcggt acccaacgat tcgcccatca agacattgga
      541 agactgtgca ggcaagacca tatcaacaga gcttgttaac tttaccaaaa aatattttgc
      601 tgaccgcaat atcccggtaa aggttgaatt ttcatggggt gcaactgaag ccaaggtggt
      661 tgagggcctg gtggatgcca ttgttgaggt tactgaaacg ggttccacaa taagagcaca
      721 cggccttaag attatttaca cattgcttga aaccaatacc aaacttattg ccaacaaaca
      781 gagcatgaag gattcagcca agcgcgcaaa aatcaaacag attgcgctgc ttttgcgagg
      841 tgccctgaat gccaataatc ttgtgggcat aaaaatgaat gtccctgaag ataagttagc
      901 tgatgttgtt aagctgatac caagcttaac ttcacccacg gtatccaaac tgtataatgc
      961 tacatggttt tcagtggaaa gcgttattca ggaatcggtg gtcagggatc ttatccctaa
     1021 actcattaat gccggagctg atggaataat tgaatacccg ctcaataaag ttgtgtcaca
     1081 ggatgatgtg tgcttaaagt aacttgttaa agggtaatgc atgcagcatg atgtaatcag
     1141 caggggaagg cagtatataa agtcctttgc tgtaacactg cctgccccac ggttttatgc
     1201 cgatagttac tggtatttag ccattgcaaa aaaaatatca acagtttcat cagtcattgc
     1261 ttactgcgct ggatgcatgc gtcaaaaagg gagcgcatta gggcacagct attaccatgc
     1321 agaaaaagta gcaattgaat cggcggcgat agttcttaag gaaaagggta tttccagcca
     1381 gtcaatacac cttgcaatgc tggcactcat tgcaggattt ctgcatgatt attaccggga
     1441 aaaaaaagac catccggcaa aagctgcaga gtatgtacag gcacatctat ctcacttcat
     1501 gcctgaaagt gatattgatg ccatcagttt tgccatacgc aatcacgaag catttaaaga
     1561 gtaccagatg gtggataaca gtgatatcat gctgctatca aatgcactgt atgatgctga
     1621 taaattcagg tggggccccg ataacttcat atacacaata tgggacatga ttgctaccat
     1681 gaatattccc atccaggata ttattgccca tttccccgga ggtgttgcac atatcaatag
     1741 cattcgttat acattcagat ccaaaacagg catggattac ggccctgaat tcattgatca
     1801 gggtatgctg atagctgaaa aattgttaca gtttttacag aacaattcat aaatttgatt
     1861 gttataagga ttataaaatg tatacgtgct gtaatagtta taagtaataa atacagtgaa
     1921 taaattgtaa aaaatcttga caaataacac ctttttttag gaatatttta ttaaaattgt
     1981 atatattgta tatttcagac aggcccacgt agctcagtcg gcagagcatt tgcatggtaa
     2041 gcaaaaggtc accagtt
//
LOCUS       DAOSZP010000432         2055 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_41132_length_2055_cov_3.456500, whole genome shotgun sequence.
ACCESSION   DAOSZP010000432 DAOSZP010000000
VERSION     DAOSZP010000432.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2055
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41132_length_2055_cov_3.456500"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(78..1841)
                     /gene="rpoD"
                     /locus_tag="PK348_08970"
     CDS             complement(78..1841)
                     /gene="rpoD"
                     /locus_tag="PK348_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010574820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HXK66385.1"
                     /translation="MAKGDVLLESIPEVKKLVDLGKTNGEITYDELNDILPDKVLNSD
                     KIDDIFILLNQLGIDVVEEPTRRAEAVLPKKEKSHSKKASAQTSETAVSDDPIRLYLK
                     EIGKVSLLSGDEEVKLAKRIEEGEILIENAVLDSTVLLNELIKNHSKVKSGKIKLTDV
                     LRINKLYYFSSFDMHDLEKRYENNMSIIIAEDKKIMQYQNKLRKLAEDSKKALELKEK
                     IAESRKIIKDALVNMQVHENEINKTAQRILSMVTRIKETRHFFKSIENHFNKSVKELK
                     QYGRKLEKNEDVKKILKELKVKTKDEILEIVKDIRNNERKIRRIEQEAGATCDEIMEW
                     GTQIEIGHRKISLAKKELINANLRLVVSIAKKYANRGMHFFDLIQEGNIGLIKAVDKF
                     EYRKGYKFSTYATWWIRQAITRAISDQARTIRIPVHMIEQINKVMRETRLFQQEYGRE
                     PSPEELADRLGWPVSKVKGVKNVAREPISLETPIGEEEDSLLGDFIEDKEVDSPVNTA
                     AYRLLSEQINAVLSTLPAREQKVIRMRFGLDDGYSHTLEEVGYVFKVTRERIRQIEAK
                     ALRRLRHPTRARKLKDYLDYM"
BASE COUNT          531 a          435 c          339 g          750 t
ORIGIN      
        1 attacgatat aatgtttgca aaatttactc aacgacctct tttgggacag ccccttaatg
       61 ctattataga agtatggcta catatagtca agataatctt tcaattttcg tgcacgggtg
      121 ggatggcgca accgacgcaa tgctttggct tctatctgtc gtatacgctc acgggttacc
      181 ttaaacacat acccaacttc ttccagtgta tgggagtatc catcatccag accaaaccgc
      241 atgcggataa ccttctgttc acgtgctggc aatgtgctca gcactgcatt tatctgctca
      301 gaaagaagcc ggtatgctgc agtattcacc ggtgaatcaa cttctttgtc ctcaataaaa
      361 tcacccaata acgaatcttc ttcttcgcct ataggggttt caagcgatat gggctcacgg
      421 gcaacattct tcacaccttt aactttactt actggccagc ctaacctgtc ggctaattct
      481 tcagggcttg gctcacggcc atattcctgc tgaaagaggc gtgtttcgcg cattacctta
      541 tttatctgct caatcatatg aacaggtata cgtatggtac gtgcctggtc agaaattgct
      601 ctggttattg cctgtcgaat ccaccatgtg gcataggtgg aaaatttata ccccttgcga
      661 tactcaaact tatctactgc ttttattaag ccaatattac cttcctgaat cagatcaaag
      721 aaatgcatac ctctgttggc atatttcttt gcaattgata ctacaagcct gaggtttgca
      781 ttaatgagct ctttttttgc cagtgaaatc ttgcggtggc ctatttcaat ctgtgtaccc
      841 cattccatga tttcatcaca ggtagcacct gcttcctgct ctatgcggcg aatcttgcgc
      901 tcattatttc tgatatcttt aactatctcc agaatttcat ctttggtctt gacttttagt
      961 tcttttaata tttttttaac atcttcattt ttttcaagct tacgaccata ttgctttaac
     1021 tcttttactg atttgttaaa atgattttca atgcttttaa aaaaatgccg cgtttccttt
     1081 attctggtaa ccatcgatag aatacgctgt gctgttttat ttatttcatt ttcatgtacc
     1141 tgcatgttta ccagtgcatc cttaataatt ttgcgggatt ctgctatttt ttcttttaat
     1201 tccagtgctt tcttagaatc ctcagctaat ttgcgtagtt tattctggta ttgcattatt
     1261 tttttgtctt cagctatgat gatactcatg ttgttttcat agcgcttttc taaatcatgc
     1321 atgtcaaaag aagaaaaata gtagagttta tttatccgca aaacatcagt cagcttaatt
     1381 ttgccacttt taacctttga atgattcttg ataagctcat tgagaagcac agtagaatcc
     1441 agcacagcat tctcaataag tatttcacct tcttctatac gttttgccag tttcacctct
     1501 tcatcaccgg aaagcaggga tactttgcca atctccttta ggtacaggcg gattgggtca
     1561 tcggaaacag cggtttctga tgtctgagct gatgcttttt ttgaatggga tttttctttt
     1621 tttggcaata ctgcttcagc acggcgagtg ggttcttcaa caacatcaat gcccagctga
     1681 ttgagaagaa tgaaaatatc atcaatttta tcagaattta ataccttatc gggcaaaata
     1741 tcgttaagct catcataggt aatctcacca tttgtttttc caagatccac aagtttttta
     1801 acttctggaa tactttctaa aagaacatca ccctttgcca taataaactc taccctttat
     1861 attaaaaatt taatgtgatc ttaaattttt gcactgcctt tgttatagat atatgaagct
     1921 aatttttctc tttcgcggcg caatacttct aattcggtca tataatattg tatattatcc
     1981 tgctgattct tcttaatttt ttctgcaaat tcgttaattt ttttgtcgat agtatacagc
     2041 cgcatattta atatt
//
LOCUS       DAOSZP010000433         2049 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_41310_length_2049_cov_3.317954, whole genome shotgun sequence.
ACCESSION   DAOSZP010000433 DAOSZP010000000
VERSION     DAOSZP010000433.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41310_length_2049_cov_3.317954"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..637)
                     /locus_tag="PK348_08975"
     CDS             complement(<1..637)
                     /locus_tag="PK348_08975"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body protein"
                     /protein_id="HXK66386.1"
                     /translation="MVRGLYTGASGMIAQEARLDAIANNLANVDKTAYKKDLTLFKAF
                     PDMIIRRLNDDGLGITPAGSYDTMPFVGKLGTGVEVNEVYTIYDQGSLMRTENPFDMA
                     LDGKGFFTVMTERGERYTRNGAFTLNQDGILVTHNGLPVLGQNGIIKLQKNNFMINER
                     GEILVNAALSLEPTDLVGMTQNNWEQPVVIDKLKIVDFENIRELKKEGESLY"
     gene            complement(637..1905)
                     /gene="murA"
                     /locus_tag="PK348_08980"
     CDS             complement(637..1905)
                     /gene="murA"
                     /locus_tag="PK348_08980"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HXK66387.1"
                     /translation="MDTYIINGGKKLNGSVTISGAKNAALPIIVASLLAEGKTVLHNV
                     PNLKDIQTILQVIKCLGADYQFDTQTNTLTIHVNSLKNAEVPYDLVKTMRASIYVMGP
                     LLARCGEADVSLPGGCAIGERPVDIHLSGFEALGSTINIEHGYIKARAKKLIGNRFIM
                     RKVSVGATANIMMAAVLADGETILENCAMEPDVVDLGNFLIKMGAKIDGLGTERIVIK
                     GVKKLKPVTYSIIPDRIEAGTFLIAGAITRSNITINKVIPEHFQACIDILSEMGFVIA
                     GGDNSITIEPSKKLQGSMIRTLPYPGFPTDLQAPIMSLMCTIPGISVIIETIFENRYT
                     HVGELRRMGADIEIEGNVAVVKGGNQLQAAPVMMSDLRAGASLVLAALAAKGQTEIRR
                     IYHTDRGYENFEQKLQSLGADIQRVKGGKP"
BASE COUNT          570 a          450 c          391 g          638 t
ORIGIN      
        1 tgtataatga ttcgccttct ttttttaatt cacgaatgtt ttcaaaatca actatcttta
       61 gcttatcaat taccacaggc tgttcccagt tattctgtgt catgcctaca agatcagtgg
      121 gttcaagcga taatgctgca ttaacaagta tttccccacg ttcgtttatc atgaaattgt
      181 ttttttgcag tttgattatg ccattctgtc caagaaccgg aaggccatta tgggttacca
      241 gtataccgtc ttgattaagt gtaaaagcgc cattgcgtgt atagcgctcg ccacgttcgg
      301 tcatcactgt aaaaaaccct ttgccatcaa gtgccatatc aaacggattt tcagttctca
      361 ttaacgaacc ctgatcataa atggtataca cttcgttgac ttcaacgccg gtacctaatt
      421 tgccaacaaa tggcatagta tcgtaactcc ctgcaggggt aatacccaat ccatcatcat
      481 tcagacggcg aattatcata tcagggaagg ctttaaataa agtaaggtct ttcttatatg
      541 cggttttgtc aacgttagcc agattgttgg caattgcatc caggcgtgct tcctgagcaa
      601 tcatgccact tgcgccggta tacagtcctc gtaccattat ggttttcctc cttttactct
      661 ttgtatatcg gcacctaagg attgtaactt ttgctcaaaa ttttcgtacc ctctgtcggt
      721 gtgataaata cgtcgtattt cagtttgacc tttagctgcc agagcagcca gtaccagcga
      781 tgctcctgca cgaagatcag acatcataac cggcgcagcc tgtaattggt ttccaccttt
      841 aacaacagca acatttcctt caatttcaat atcagcaccc atacggcgca gttccccaac
      901 atgagtatac cggttttcaa agattgtttc gataataacg ctgataccgg gtatggtgca
      961 catgagtgac attataggcg cctgaaggtc ggttgggaag ccgggatacg gcaatgtcct
     1021 gatcatgctg ccctgcaatt ttttagatgg ttctattgta atggaattat cgccgcccgc
     1081 aataacaaaa cccatttcag acaatatatc aatacaggcc tggaaatgct cagggataac
     1141 tttgtttatg gtaatgttac tacgtgtaat agctccagca atcagaaatg ttccagcttc
     1201 aatgcggtca ggaataattg aataggttac cggtttaagc ttttttacgc cttttataac
     1261 gatgcgttca gtgcccaatc cgtcaatttt tgctcccatt ttgattaaaa aatttcctaa
     1321 atcaacaaca tccggttcca tggcacagtt ttccaatatt gtttcgccat cagcaagtac
     1381 agctgccatc atgatattag cagtagcgcc aacagaaacc tttcgcatta taaaacggtt
     1441 cccaatcagt ttctttgcgc gtgctttgat atatccatgt tcaatattaa tggtgctgcc
     1501 taaagcttca aaacccgaaa ggtgaatatc aacaggacgc tccccaatag cacatccacc
     1561 aggcagcgat acatcagctt ctccgcatcg agccaaaagt ggtcccataa cataaatgga
     1621 ggcgcgcatg gttttaacaa ggtcataggg cacttcggca tttttcagac tgttgacatg
     1681 aatggttagc gtattcgttt gggtatcaaa ctggtaatca gctcccaggc atttgataac
     1741 ctgcaaaatt gtttgaatat cttttaaatt gggaacatta tgtaaaacgg ttttgccttc
     1801 agcaagaagg cttgctacaa taataggtaa tgcggcgttt ttagcaccgg agatagttac
     1861 tgaaccattg agctttttgc cgccattgat gatatacgta tccacagttt cctcacattt
     1921 tgtattgatt ttatttgtga gcagtagtaa taatgtagca aattgtcaat tcaattaaaa
     1981 gattttatct tcaaaattaa tgtacagtcg ctatcacaaa tatagtcgtt tgaggaattt
     2041 tataaccgt
//
LOCUS       DAOSZP010000434         2041 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_41526_length_2041_cov_2.028197, whole genome shotgun sequence.
ACCESSION   DAOSZP010000434 DAOSZP010000000
VERSION     DAOSZP010000434.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2041
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41526_length_2041_cov_2.028197"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            133..555
                     /locus_tag="PK348_08985"
     CDS             133..555
                     /locus_tag="PK348_08985"
                     /inference="COORDINATES: protein motif:HMM:NF017020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HXK66388.1"
                     /translation="MSKEDNIQESLRWFDTAMSDYEAAKIMCENKKYAHACFLSQQSA
                     EKALKALHYYYDSDPWGHSVLKLIDELKYIDIEVYTILALYTDNARLLDKLYIPTRYP
                     NGLPDITPDLAYSKKDADEALLSADMILQKVKEIIAKR"
     gene            619..1098
                     /locus_tag="PK348_08990"
     CDS             619..1098
                     /locus_tag="PK348_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012176282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HXK66389.1"
                     /translation="MASTQCEVPAFNPEDSEIKEILSQYKIIAVVGLSDDPSKDSNRV
                     AQYLIHHGYTVVPVNPTKETILGLKCYPNLKSIPFPVDIVDIFRKPADVPPVVDDAIA
                     IGAKVVWMQLGIAHNEAAKKAKEAGLKVVQSKCIKIEHTRYFGTGQETVSFNINLNQ"
     gene            complement(1082..>2041)
                     /locus_tag="PK348_08995"
     CDS             complement(1082..>2041)
                     /locus_tag="PK348_08995"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HXK66390.1"
                     /translation="VAEGAGGPRAVKYGTTKDYITGLEFVLTDGSIIQHGGKFIKNAT
                     GYNLTGILIGSEGTLAVITKIFCRLIPAPAHTLDMLIPFQSLHDAVQMVYTLLINRIR
                     PAVLEFMEEDALTLAAQYLGQDMPHPQARAHLLIQLDGDSYHEIDESLQKILSLGAVD
                     PNSILVAQSPEQKERLWKARRCIREAIHAKSPVFLAEDCSVPRSKIVPFLTGVKQALT
                     PYGLQSIMFGHAGDGNVHIDVLKNDMPYDEFKALVPSLKEIIYAEAYKYGGTITGEHG
                     TGFIRKDALKKFASSQELSLYKRLKKAFDPNGILNPHKIIDLD"
BASE COUNT          650 a          371 c          416 g          604 t
ORIGIN      
        1 atgatgattt taatgatata tacaatattg taaataatca ctgtgactta cttatttata
       61 ctccttcaga atgggaaaat attcgtcaaa gaaaattttt taaaaatata ctgaaagaag
      121 caaaagaaat atatgtcaaa ggaagataat atacaagaat cactccgttg gtttgataca
      181 gcaatgagcg attatgaagc tgctaaaata atgtgtgaaa acaaaaagta tgctcatgcg
      241 tgttttttaa gccagcaatc agctgaaaaa gcattgaaag cattacatta ctattatgat
      301 tcagatccat gggggcattc ggttttaaaa ttaattgatg agttgaaata tatagatatt
      361 gaagtatata ctatattagc attgtatact gataatgcac gtttattaga taaattgtat
      421 ataccaacac gttacccaaa cggtttgcct gacattacac ctgatctagc atattcaaaa
      481 aaagatgcag atgaagcatt attgagtgct gatatgattt tgcaaaaagt taaagaaatt
      541 atagcaaaaa ggtaatttta tacttgccgg ttcaaaggta tcatagtatc ttaataacaa
      601 ttttttcagg agttttttat ggccagtaca caatgtgaag ttccagcatt taatccagag
      661 gatagtgaaa taaaagaaat cctttcacaa tacaagatta tagctgttgt tgggctatcc
      721 gatgatcctt ctaaggatag taacagggtg gcgcagtatt tgatacatca tggatacacg
      781 gtggttccgg ttaatccaac aaaagaaaca atactgggac ttaaatgtta tcctaattta
      841 aaatccatac ctttccctgt ggacatagtg gacattttcc gcaagcctgc cgatgtaccg
      901 ccggtggtgg atgatgctat tgccattggg gcaaaagtcg tatggatgca gctgggcatt
      961 gctcataacg aagcagccaa aaaagctaaa gaagcaggtt taaaggtggt gcagagcaaa
     1021 tgcatcaaaa tagagcatac ccgctatttt ggaacgggac aggaaacagt ctcatttaat
     1081 attaatctaa atcaatgatt ttgtgagggt ttaaaattcc attgggatca aacgcttttt
     1141 ttaagcgctt ataaagcgat agttcctgtg atgaggcaaa tttttttaac gcatctttac
     1201 gtataaatcc agttccatgc tctccagtga tagtcccgcc atatttatat gcttctgcgt
     1261 aaataatctc ttttaatgat ggtactaacg ccttgaattc atcatatggc atgtcatttt
     1321 taagcacatc gatgtgaaca ttgccatcac cagcatgacc aaacatgatg gattgaagcc
     1381 cataaggggt cagagcctgt tttacacccg tcaaaaatgg cactatcttt gagcggggta
     1441 cagagcaatc ttcggccaga aaaacaggac tttttgcatg tattgcctca cgtatacagc
     1501 gccgtgcttt ccacaggcgc tctttttgtt caggagattg tgctaccaat atgctgttgg
     1561 gatcaactgc gccaagtgaa aggatttttt gtaacgattc atcaatttca tggtaactat
     1621 cgccatctaa ctggatgagt aagtgggcac gagcctgagg atgtggcata tcctgcccca
     1681 aatactgtgc agcaagggtc agagcatctt cttccataaa ttcaagcacc gcaggccgta
     1741 tgcgattgat taaaagtgtg tagaccatct gcacggcatc gtgaaggctt tgaaacggta
     1801 tgagcatgtc aagcgtatgt gcaggagcag gaataagcct gcagaagatt tttgtaataa
     1861 ctgcaagcgt gccttctgaa ccaatgagta tgccggtaag attataaccg gttgcatttt
     1921 ttataaattt acccccatgc tgtatgatac taccgtcagt taaaacaaat tcaagcccgg
     1981 taatgtaatc ttttgtagtg ccgtatttaa ctgcccgtgg tcccccggct ccttcggcaa
     2041 c
//
LOCUS       DAOSZP010000435         2039 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_41556_length_2039_cov_2.972782, whole genome shotgun sequence.
ACCESSION   DAOSZP010000435 DAOSZP010000000
VERSION     DAOSZP010000435.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41556_length_2039_cov_2.972782"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            35..376
                     /locus_tag="PK348_09000"
     CDS             35..376
                     /locus_tag="PK348_09000"
                     /inference="COORDINATES: protein motif:HMM:TIGR00589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HXK66391.1"
                     /translation="MQPPQCDLSLYTSNEQKVLKELLLIQPGNTISYGELAAQCGFNE
                     GARFVGNVMASNIFPVLFPCHRVIRSNGDTGNYSGGIKIKEFLLAWEKENENSSVKQR
                     TIHHAEYTNKK"
     gene            complement(380..>2039)
                     /locus_tag="PK348_09005"
     CDS             complement(380..>2039)
                     /locus_tag="PK348_09005"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HXK66392.1"
                     /translation="LFIQLAGTVIVAIVASLIVAVIFVPWCLRMTALHPLPSSHISII
                     QRLNLHIPRITLVAMFLGTCKKYVSITYVNSTMEYFFNNQKKLYGIILLALISTVIPI
                     SNITVEDISFTDQNKLFARLEMPSGSSLQATSHVALYIEKQLQHITSINNISTRIQQE
                     HADFIIDTDGTISSEMIKRHLQLPLDTSLIFTSTGGTIRNNIEVIIKGKDVPVIRQLA
                     YRFAEQLQATSAVEDIIYHFKDERPEVLIQFDRMKCAYSGIPIAYAGDYIRNLFYGPV
                     ITKYIDDREVDVRIKDNQYTVNEIDDIVLPMDNKAVPLKNIATVATSHIVTTVWHHDK
                     MRSETISIIPKVPLYKMETILHNTIQQLSLPDGYYIEYDKTFALHKQSIIYMVIYICI
                     AIVSVYMVIASIFESFMLPWIIIVSIPIAWIWAVWALYIFNIQCNISTIMGFVVLTGI
                     VVNNSILLIDSYVQTIKSSRKKKLLLIKDYKTINQHRSKPMLITTITTVSGLLPLVIS
                     SGGSHLWQGFALTVITGLCGSFACILIITPILFNTYQKNLNTQQ"
BASE COUNT          659 a          326 c          389 g          665 t
ORIGIN      
        1 tacatttttt aagcagtatt aacgaaagat atttatacaa cctccacaat gcgatttatc
       61 cctctataca agtaatgaac agaaagtttt aaaggaatta cttttgatac aacctggcaa
      121 tactatatcg tatggtgaac ttgcagcaca atgcggtttt aatgagggtg cacggtttgt
      181 gggaaatgta atggcaagca atatatttcc tgttttgttt ccctgccata gagtaatacg
      241 aagtaatggt gatacaggca attacagtgg tggtataaag ataaaggagt ttttacttgc
      301 gtgggaaaaa gaaaatgaga attccagcgt taaacaaaga acaatacatc acgcagagta
      361 tacgaataaa aaataaatct tactgttgag tatttaaatt tttttgataa gtattaaata
      421 aaattggtgt aataatcaat atacatgcaa atgaaccaca cagtccggtt atcactgtta
      481 aagcaaaacc ctgccataga tgacttccac cagaagagat aaccagcggc aacaaaccag
      541 atacagtggt tattgttgtt atcagcattg gtttgcttcg atgctgattt atggttttat
      601 aatctttgat cagtaataat ttcttttttc ttgaagattt gatcgtttga acataactat
      661 ctatcaaaag tattgaattg ttaaccacaa taccggttaa tactacaaac cccataatgg
      721 ttgatatatt acactggata ttaaagatat ataatgccca gactgcccat atccatgcta
      781 ttggtatgga tacgatgatt atccatggta gcatgaacga ttcaaatata gaagctatta
      841 ccatgtacac tgagacaata gctatgcata tatagatcac catatagatg atggattgct
      901 tatgcagtgc aaaggttttg tcatattcta tgtaatatcc atcggggagg gataattgtt
      961 gaatggtgtt gtggagaatt gtttccattt tatacagggg aacctttgga ataatgctga
     1021 ttgtttcaga tcgcatctta tcatgatgcc atactgtggt aacaatatgt gatgtggcaa
     1081 ctgttgcaat attctttaat gggacagcct tattatccat aggtaatact atatcatcaa
     1141 tttcattaac agtatactgg ttatctttta tgcgtacatc aacttccctg tcatcaatgt
     1201 atttggttat cacaggacca tagaaaagat ttcttatgta atcgcctgca taagcaatgg
     1261 gaatcccgga gtatgcgcat ttcattctgt caaattgaat caacacttcg ggacgctcat
     1321 ctttgaaatg atagataata tcttcaactg ctgatgttgc ctgcaattgt tcagcaaatc
     1381 tatatgctaa ctggcgaata actggcacat ctttcccttt tatgataact tcaatgttat
     1441 tgcgaattgt gccacctgtg ctggtaaaaa ttaatgaagt atccaatgga agctgtaaat
     1501 gccgttttat catttcactg cttattgtgc catctgtatc aataataaaa tctgcatgtt
     1561 cctgttgaat tcgcgttgag atgttgttaa tggatgtaat atgctgcaat tgcttttcta
     1621 tataaagggc aacatgggaa gttgcctgta atgatgaacc tgagggcatt tccaatcgtg
     1681 caaagagttt attttgatca gtaaagctaa tatcttcaac agtaatgttt gagattggga
     1741 ttacggtgct gataagtgct agcaggatta tgccatagag tttcttttga ttattgaaaa
     1801 aatattccat agttgagtta acataggtga tagatacata ttttttgcat gtaccaagaa
     1861 acattgcaac taatgtaata cgcggaatat gcagatttaa tcgttgtatt atagatatat
     1921 gggatgatgg tagtggatga agtgctgtca tgcgtaaaca ccagggtaca aatataacag
     1981 ccactatcag tgatgcaact atagcaacta tcacagtgcc agctaactgt atgaataac
//
LOCUS       DAOSZP010000436         2037 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_41609_length_2037_cov_3.437437, whole genome shotgun sequence.
ACCESSION   DAOSZP010000436 DAOSZP010000000
VERSION     DAOSZP010000436.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2037
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41609_length_2037_cov_3.437437"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            248..1186
                     /locus_tag="PK348_09010"
     CDS             248..1186
                     /locus_tag="PK348_09010"
                     /inference="COORDINATES: protein motif:HMM:NF024122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HXK66393.1"
                     /translation="MKKFTISLPVLINFIFLISLVVSCTQQEKHVDIILSNILSSKTA
                     IEQYITKQSTAKSAHPIFVTFWDFDGTILKGDCSDGYKENDRQIYRGLVQIAIEQNFS
                     KLYKENEFDVFSNYYEHLDRTQGHLAAYTYLTTIFAGQNEDAIIKLSTQYFNETLQHY
                     YFSSSIAIMNELIKNNIHIYIISASPDFFVKGAAQSLKINPDAIHGIKLKNVNGILTN
                     TIVPPVTYAKGKTMKVLQIMKHLQQKYSTDHVYALAAFGNSYHTDGPFLEYVAKQKLP
                     AGTPVAVMINGGKEPDAYKGLFYCVKQSKIIQYSYK"
     gene            1205..>2037
                     /locus_tag="PK348_09015"
     CDS             1205..>2037
                     /locus_tag="PK348_09015"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HXK66394.1"
                     /translation="MKLFHFCIFIIFILTKPAYPEMWGYYLKDSLPYEKHQQYWENRF
                     ANLTVLCFTGITVSSTDCIIPEFTNEQALFKKAYERSIALIPHVTFTSVKSGIAFLTH
                     PQYWKKIIHTLAYAIRTSGWAGCHFDFEYLPSKYAANFAQFLKIFKAIAPDISLSAAM
                     FPQVEYNPTHAAFHDYALLSTYLDAIVLMCYDQHNPKTKPGPVTGVEWSRKNIEYALQ
                     FFKANQLWLGIPAYGYIWKDNTYYSVITMKSLSRYVTRYRNYRHTSGTIVIEYTQNND
                     H"
BASE COUNT          722 a          385 c          318 g          612 t
ORIGIN      
        1 gctgaagaga aatacaggta gtagtttcaa aatacatcgt ttcctcccaa agtatatatt
       61 tttatcacgg tttattgtaa cgaaattata gtatcgataa ctatcgttgc aacaattttt
      121 tatatatatt aagaacaata ccaaaaaaga agttgaaaaa tggtaatgac ttattaaaat
      181 aaatgtttta ctgaataagt ataaaatatt tgtagttatt caaaaatact gattaagaaa
      241 atatactatg aaaaaattta ctatctcact acctgtactg attaacttta tctttctaat
      301 tagtttagtt gtatcatgca cacagcagga aaaacatgtt gatataatcc tttcaaatat
      361 cctttcatca aaaactgcta tcgaacagta tataacaaaa caatctacag ctaaaagtgc
      421 tcatccaatt tttgttactt tctgggattt tgatggcaca attttaaagg gagactgttc
      481 tgacggctat aaagaaaatg acaggcaaat ctacagggga ctggtacaga tagcaataga
      541 acaaaatttt tcaaagttat ataaagaaaa tgaatttgat gtgttttcca attactatga
      601 gcatcttgat agaacgcagg gtcaccttgc agcatataca tatctaacga caatctttgc
      661 aggacaaaat gaagatgcta tcattaaatt atccacacag tattttaatg aaacgttaca
      721 gcattactat ttttcttcat caatagcaat catgaacgag cttattaaaa ataatattca
      781 catctacatc atttcagcaa gtcctgattt ttttgttaaa ggtgctgcac agtcactaaa
      841 aataaatcct gatgccatac acggcatcaa attaaaaaac gttaatggca tactaacaaa
      901 caccatagtt cctcctgtta cctatgcaaa aggcaaaaca atgaaggttt tacaaattat
      961 gaagcattta cagcaaaaat actcaacaga tcatgtatat gcacttgcag cttttggtaa
     1021 cagctaccat accgatggac catttttaga gtatgttgcc aaacagaagt taccagcagg
     1081 cacgccagta gctgttatga ttaatggtgg caaagaacct gatgcatata aaggtctatt
     1141 ctattgtgtt aaacaaagta agattataca atactcatac aaataattat atacagcaca
     1201 atacatgaag ctatttcatt tttgcatctt tattattttc atactgacaa aacctgcata
     1261 ccctgaaatg tggggctact acctgaaaga ttcactgccc tatgaaaaac atcagcaata
     1321 ctgggaaaat cgttttgcca atcttactgt gctgtgtttt acgggaataa ccgtgagcag
     1381 tacagattgc attattcctg aatttactaa cgagcaagca ctttttaaaa aagcctatga
     1441 gcgcagcata gccctcatac cccatgtaac ctttaccagt gttaaaagtg gcattgcatt
     1501 tttaactcat ccacaatact ggaaaaaaat aatacacaca ctggcttatg caatacgtac
     1561 cagcggctgg gcaggatgtc attttgattt tgaatacctg ccatccaaat atgcggcaaa
     1621 ctttgcccaa tttttaaaaa tatttaaggc gatagctcct gacataagcc tatcggcagc
     1681 aatgttcccg caggttgaat ataatcccac ccatgctgca ttccacgatt atgcacttct
     1741 tagcacctat cttgatgcaa tcgttttgat gtgctacgac cagcacaatc caaaaacaaa
     1801 accagggcca gtaacaggtg ttgaatggtc caggaaaaat attgagtatg cgttacaatt
     1861 ttttaaagcc aaccaactat ggttaggcat tcctgcctat ggttacatat ggaaagataa
     1921 cacgtattac tccgttatca ccatgaagtc attgtccaga tatgtaacac gttaccgtaa
     1981 ctatcgccac acttcaggaa ccattgttat agaatatacg caaaataatg accattt
//
LOCUS       DAOSZP010000437         2017 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_42150_length_2017_cov_3.159021, whole genome shotgun sequence.
ACCESSION   DAOSZP010000437 DAOSZP010000000
VERSION     DAOSZP010000437.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42150_length_2017_cov_3.159021"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(161..313)
                     /locus_tag="PK348_09020"
     CDS             complement(161..313)
                     /locus_tag="PK348_09020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66395.1"
                     /translation="MVGYLDLQRKMLEGFFAYFRVENLYNNRYYLRENYPESGIKGLF
                     GIRILL"
     gene            complement(440..1600)
                     /locus_tag="PK348_09025"
     CDS             complement(440..1600)
                     /locus_tag="PK348_09025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66396.1"
                     /translation="MGYHVTIKKLCIIIALFFYLVQPVLAQESNAQSTDAQNYRNMNI
                     DMQVVYGQYNDMLSTVNLSQEQEGFVYLLRTYLKKSNDYGYKDDIYKNTSYYQNRIGF
                     TGNVTASETWKSIFDISVHNDSYGMAQNPVYSREEKDNVILDWKNIVKYSPSFEMYYG
                     IGGAQYYHRLVGTTTQGEKSRVYQGHGQIGGEYIWSASNRFKYLSTFYGYSYEQYEND
                     FFCNNEIVDDFNITKDVGVTIGLNADFNRDDHWLLGPIIGASYKGFSNSSFILQYSYF
                     MQPFKPEELYLQQKFIAPIYNLEPAHVHKVHAKIDYKMNSLITMQVVSQIEHSNNFYN
                     YVTVPGDVLTAQGIESWIVTNSVMLVLNIVPKVLNVDAQYTYAYYHADEHIT"
     gene            complement(1705..>2017)
                     /locus_tag="PK348_09030"
     CDS             complement(1705..>2017)
                     /locus_tag="PK348_09030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66397.1"
                     /translation="TYHFRDNWDVTWKQYITDLGKYIGKSPQGNIPFGVAWYSATVLE
                     SLCNLLHIRSPLTRLAVAVMGRDNDVDTTLTQTELGWETMVAYDDAMQHIAAHTFKQI
                     P"
BASE COUNT          605 a          420 c          340 g          652 t
ORIGIN      
        1 cggtaaaaaa ttattcgttc aacgattgca gcaacagcta tcaccgaaca tagcaatatt
       61 ggtaaaagca tcacccatgt tggcacggca tcaaatacgc caattaatgt accactgtgc
      121 atattatttc atcctgtgat tatttactat gaactatcgc ctataataat atccgtatgc
      181 caaacaaacc cttgatccct gattccggat aattttcgcg aagataatag cgattgttat
      241 acaaattttc cactctaaaa tatgcaaaaa agccttcaag catttttcgc tgtaaatcca
      301 gatacccaac cacccgcgat ggaagtttgg tatcataaac gggcgaagta tatactgaac
      361 ttgcaatatc atgggaaaga ttaattttcc actttgttcc attataggtt attgcaaagg
      421 ctaccagatg ctgtggtctt taggttatat gttcatcagc atgataatat gcataggtat
      481 actgtgcatc aacattgagc actttgggca caatattgag taccagcata acactattgg
      541 taacaatcca tgactcaata ccctgagcag tcaacacatc gcccggaaca gtcacataat
      601 tgtaaaagtt attactgtgt tcaatttgcg ataccacctg cattgttatt aagctattca
      661 ttttataatc aatcttagca tgaactttat gaacatgcgc cggctcaagg ttatatattg
      721 gtgctataaa tttctgctgt aagtataatt cttctggttt gaacggctgc atgaagtaac
      781 tgtactgtaa tatgaagctg gaattactga atcccttata acttgctcca ataattggcc
      841 ccagcaacca gtgatcatcc ctgttaaaat ctgcattaag gccaattgtt actccaacat
      901 cctttgttat attaaagtca tcaactatct cattgttaca aaaaaaatca ttttcatatt
      961 gttcgtatga gtagccatag aacgtagata aatatttaaa acggttggaa gcagaccata
     1021 tgtattcacc cccaatctga ccgtgtccct gatatacgcg gcttttttcg ccctgcgttg
     1081 ttgtgccaac cagcctgtgg taatactgtg caccgcctat gccataatac atttcaaacg
     1141 aagggctgta ctttactata tttttccagt caagaataac gttgtctttt tcttcacggc
     1201 tatatacggg attttgtgcc ataccgtatg aatcattatg cacacttata tcaaaaattg
     1261 atttccaggt ttctgaagca gttacattcc cggtaaaccc aatacgattc tggtagtagc
     1321 ttgtgttttt atatatgtca tccttgtatc cataatcatt ggattttttt aaatatgtcc
     1381 gcagtaaata tacaaatcct tcctgctcct gtgaaaggtt aactgttgac agcatatcgt
     1441 tgtactgccc atacaccact tgcatatcta tattcatgtt gcgatagttc tgggcatcag
     1501 tactctgagc attactttcc tgagcaagga ctggttgcac cagataaaag aaaagtgcaa
     1561 taattataca taattttttt atagttacgt gataccccat acaaccccat atatttgtat
     1621 atagaaatat agtttttata tactgtaaca ttacttgtga tgaaggttga ttatttatat
     1681 gatgcggttt taagtcaata caatttaagg aatttgctta aaggtgtgcg ctgcaatgtg
     1741 ctgcatagca tcatcatacg ccaccattgt ttcccaccct aattcagttt gcgtcaatgt
     1801 agtatcaaca tcattgtccc tgcccatcac tgccactgca agccgtgtta acggcgaacg
     1861 gatatgcaaa agattacaca ggctttcaag tacagttgct gaataccatg caacaccaaa
     1921 aggtatattg ccctgtgggc ttttgcctat atattttcca agatcagtta tatattgctt
     1981 ccaggtgaca tcccagttat ccctgaaatg atacgtt
//
LOCUS       DAOSZP010000438         2016 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_42182_length_2016_cov_3.403876, whole genome shotgun sequence.
ACCESSION   DAOSZP010000438 DAOSZP010000000
VERSION     DAOSZP010000438.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2016
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42182_length_2016_cov_3.403876"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..659)
                     /gene="argC"
                     /locus_tag="PK348_09035"
     CDS             complement(<1..659)
                     /gene="argC"
                     /locus_tag="PK348_09035"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: protein motif:HMM:NF013298.2"
                     /note="catalyzes the reduction of N-acetyl-5-glutamyl
                     phosphate to N-acetyl-L-glutamate 5-semialdehyde in
                     arginine biosynthesis and the reduction of
                     N-acetyl-gamma-aminoadipyl-phosphate to
                     N-acetyl-L-aminoadipate-semialdehyde in lysine
                     biosynthesis; involved in both the arginine and lysine
                     biosynthetic pathways; lysine is produced via the AAA
                     pathway, lysine from alpha-aminoadipate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HXK66398.1"
                     /translation="MKKALIVGATGFGGLGLIEILLRHKGFQIAQLVARKDVGKKISE
                     VFPHLEGFCDMPVQSPEEINYDDIDIAFFSTPDKAGMQMIHTFYKKNIPVIDFSGDFR
                     FATVDDYTIYAKNKGMNTEHASPDVLAHTVYGLPEKYAEKIKKAKIVGNPGCFAIAMI
                     LGLLPAVETGIIDSETIICDGKTGVSGAGKNPGEANFYPQRYEDVNTYREGHHQHIVE
                     V"
     gene            1251..>2016
                     /locus_tag="PK348_09040"
     CDS             1251..>2016
                     /locus_tag="PK348_09040"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015773939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HXK66399.1"
                     /translation="MKKYNVVILGATGAVGIEFRKILLQRNFPINNIRFLGKTTVGQT
                     IEFGDRKVTVEPVTDDAFKGFDIGLFSAGASISREVARKAAESGCIVIDNSSAWRMEK
                     DVPLVVPEVNPNDVDWHNGIIANPNCSTIQMVVALKPIHDVAKIKRIVVNTYQAVSGT
                     GLKAIEELQIQVKDIMNGKPISQRKVYPHQIAFNVLPHIDVFLENGYTKEEMKMVNET
                     KKIMGDSSIQVTATTVRVPVFYGHSDSVNIETEKKIT"
BASE COUNT          667 a          356 c          382 g          611 t
ORIGIN      
        1 tcaacttcca cgatatgctg atgatgccct tcacgatagg tattgacatc ttcataccgc
       61 tgaggataaa aattagcttc acctggattt ttgcctgcac cggatacacc tgttttgcca
      121 tcacagatga ttgtttcaga atcaatgatg cctgtctcaa ctgcaggtaa taatcccaaa
      181 atcatagcta tggcaaaaca gcctgggttg cccacaattt ttgctttttt aattttttct
      241 gcatattttt cgggcaatcc atacacagta tgtgcaagta cgtcagggga agcgtgttcg
      301 gtattcatgc ctttattttt ggcatagata gtgtagtcat caacagtggc aaacctgaaa
      361 tcaccgctga agtcaataac cgggatgttt tttttataaa atgtatgaat catctgcatg
      421 cctgctttat caggagttga gaaaaatgca atatctatat cgtcgtaatt gatttcttcg
      481 ggtgattgta ctggcatatc gcaaaaacct tcaagatgcg ggaacacttc tgatattttt
      541 ttgccaacat ccttgcgggc aactaattgt gcaatctgaa aacctttatg acgcaaaaga
      601 atttcaatca accctaagcc gccaaatccg gtagcaccaa caattaatgc ttttttcata
      661 aatgttcctc cagggataga attttatcca aagtaagtaa ttgctgtcca atcattgcta
      721 aaagcgcttt tttagcagtc aatagaaaat tatcacatct ataatatcac tttatgatgg
      781 gcatagcaac aacgcaattc cttccatacc aggtcattgc gagtgcaacg aagcaatccc
      841 agatttatca aagagattgc cacgtccctt cgagactcgc aatgacgtga tcctatcgtt
      901 attgcgagtc ttgattgtat tgggatgacg aagcaatctt gtttttaaga tttagattgc
      961 ttcaccactc cgggatttgc aatgacaaaa tcttgatgta ttgtttattt aacctgaaat
     1021 ttagttaaaa gtgactatcg cacaaaatga taaacattat taatacgtta tgagtaaagc
     1081 gaaaatattt gtcaattgcg aaatacaatt atggtaatga ttttgaaata attaatgtga
     1141 aaaataattt tttatgacta attgataaaa taaaaaagtt attattgaca aaaaaaagtg
     1201 ctatatatga caaatacaca ataagtaaaa atgcaggggg atcattcatt atgaaaaaat
     1261 acaatgtggt aattttaggt gcaacaggtg cagttggcat tgaattcaga aaaattttat
     1321 tgcaacgcaa ttttcctatt aacaatattc gcttcctggg aaaaactact gttggacaaa
     1381 caatagagtt tggtgatcga aaggttactg ttgaaccggt aacagatgat gcttttaaag
     1441 gattcgatat tgggcttttt tctgcaggtg cttccataag tagagaagta gctcgaaaag
     1501 ctgctgaatc cggctgtatt gttattgaca atagttcagc ctggcgaatg gaaaaggatg
     1561 tccctctggt ggttccggaa gttaacccaa atgatgttga ctggcacaat ggcattatcg
     1621 ctaatcccaa ttgttctaca attcagatgg tagttgctct gaagcctata catgatgttg
     1681 caaagataaa acgaatagtt gtaaatacct atcaggctgt ttccggaact ggccttaaag
     1741 caattgagga acttcagatt caggtaaaag atattatgaa tggaaaaccc atatcacagc
     1801 gaaaagtata tccacatcag atagcattta atgtgttacc acacattgat gtattccttg
     1861 aaaatggata cacaaaagaa gaaatgaaga tggttaatga aactaaaaag attatgggcg
     1921 atagctcaat acaggtaact gcaactacag taagagtccc agtattttat ggccactcag
     1981 attcagtaaa tattgaaacc gagaagaaga taacac
//
LOCUS       DAOSZP010000439         2013 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_42259_length_2013_cov_5.977528, whole genome shotgun sequence.
ACCESSION   DAOSZP010000439 DAOSZP010000000
VERSION     DAOSZP010000439.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42259_length_2013_cov_5.977528"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..937)
                     /locus_tag="PK348_09045"
     CDS             complement(<1..937)
                     /locus_tag="PK348_09045"
                     /inference="COORDINATES: protein motif:HMM:NF012956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HXK66400.1"
                     /translation="MNTVNILASAYYKGAIHSVSSASGDNKADNVLNDDIESFWCTNK
                     SHSATGEYIVIDYGVAVAVNVIELLPSPSGKTTFPSDFRLESSADGSNWEVIKTENRY
                     SLDEDRYRVLLPMNAFQFLKLYIIKPKRIAAKYFSEIGGIKVGIQGASAITASSCSSY
                     EHDPSNLLMNDSAKYWESETSNTPSRQWIEIDMGRDFPLAHIGLISTSSAELFPEQLS
                     IESSEDRKIWTTIAELKRFKAEANRSYIFGMYQFSARYIRILCTTVQSSKQNYCAQIA
                     QICAYASLPSDSHFHVNAAVQYATIFQPGIVTLAGD"
     gene            1446..>2013
                     /locus_tag="PK348_09050"
     CDS             1446..>2013
                     /locus_tag="PK348_09050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66401.1"
                     /translation="MSSPIIEEKTPKGYVFKINTVKRQYVLFNQKGEIIYSDENKTKP
                     FHSMKTIVFIGVYPGDAAIKQPYQLFKIQNASIIIPYMFENNTRHYIFIPCDSYLDVI
                     PCMVEYANDIASFISFVKKGITPDFIIINSELSNDDNAMIQSLCPSSHVEIAGKSVEP
                     PNNDSKRAADAGNIDINLLSNNPVFLARV"
BASE COUNT          646 a          370 c          314 g          683 t
ORIGIN      
        1 catcgcctgc taatgtaaca atccctggct gaaaaatagt tgcatattgc actgcagcgt
       61 taacatggaa atggctatca gaaggcaatg atgcatatgc gcaaatctga gcaatctgag
      121 cacaatagtt ttgtttggag gactgtactg ttgtgcaaag tatgcgtata tagcgtgcac
      181 taaactgata cataccaaaa atatatgaac ggtttgcttc agctttaaat cgttttaatt
      241 cagctatcgt tgtccatatc ttcctgtcct cacttgattc tattgaaagc tgctcgggga
      301 ataattctgc actggatgtt gaaataagac caatatgagc aagaggaaaa tcccttccca
      361 tatcaatttc aatccactga cgagatggtg tattgcttgt ttcagattcc caatattttg
      421 cagaatcatt cattaataaa tttgatggat catgctcata tgatgaacat gaacttgcgg
      481 taattgcact tgctccctgt ataccaacct ttatcccccc tatctccgaa aaatattttg
      541 cagctattcg ctttggttta atgatataca gtttcaaaaa ttggaatgca ttcattggca
      601 gcaatacacg atacctatct tcatcaagcg aatatctgtt ttctgttttt attacctccc
      661 agttgctgcc atcagcactt gattcaagtc tgaaatctga tgggaatgtt gttttacccg
      721 atggtgaagg aagcaactct atgacattaa cagccacagc cacaccataa tcaataacaa
      781 tatattcacc tgtagcacta tgtgacttat ttgtacacca gaagctttct atatcgtcat
      841 ttaaaacatt gtctgcttta ttatcaccag atgcactgga tactgaatgt attgcccctt
      901 tataataagc tgatgccaat atattaactg tattcatcgt ttattccccc cgcagaaaat
      961 aaaattattg ccttgatatt cttgtgtact aatgtgtaaa aaatcatcta aattaaaaac
     1021 cacatatttt cttttaataa taaataaaag acctaacatt tgcttgattt tttatcaatt
     1081 actatttacc tgttaccata tcttttttac cttttatggg cttttgttaa agttggtttg
     1141 tatgcataga ttgtatgcat agagcagttg catcatcttt gataattgaa aaactcctga
     1201 acttgcattt gcagtaaaaa agttttactt ataagttatg cccataactg tagtacatac
     1261 taagtttttg ttgtgctcat gatggtatct ataccatcat gcatgtagat cgtttataag
     1321 cgctgtgatt actatcatgt attacctgtg tacactgttt tatttatgag taaaatttca
     1381 atgaaaattt atatacaatt actatcaatt actatcaatt actatcaata aagaaggtgt
     1441 aatgcatgtc atcaccaata attgaagaaa aaactcccaa aggttatgtt tttaaaataa
     1501 atactgtaaa aaggcaatat gtattattta atcagaaagg agagatcatc tattctgacg
     1561 aaaacaaaac aaaaccattc cactccatga aaacaattgt atttattggt gtttatcctg
     1621 gtgatgcagc tataaagcaa ccatatcagc tttttaagat acaaaacgcc tcaataatta
     1681 ttccctatat gtttgaaaat aatacacggc actatatctt tattccatgc gatagctatc
     1741 ttgatgtgat accctgtatg gtagaatacg ctaatgatat tgcatcattc atcagttttg
     1801 taaaaaaggg gattacgcca gacttcatca ttataaatag tgaattgagc aatgacgata
     1861 atgccatgat acaatctctt tgtccatcgt ctcatgttga aattgcaggc aaatctgttg
     1921 aaccaccaaa taatgattca aaacgtgctg cagatgccgg caatattgat attaatcttt
     1981 tatcaaataa tcctgtattt ttagcgcgtg tcc
//
LOCUS       DAOSZP010000440         2003 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_42532_length_2003_cov_3.308008, whole genome shotgun sequence.
ACCESSION   DAOSZP010000440 DAOSZP010000000
VERSION     DAOSZP010000440.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42532_length_2003_cov_3.308008"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1210
                     /locus_tag="PK348_09055"
     CDS             <1..1210
                     /locus_tag="PK348_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011639761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HXK66402.1"
                     /translation="KRVEELRNVDVRSVCYMRIPKIVEADPEQKHFILNDFHFGTISR
                     RLYDQHLCNYIPITYHQGPRVIRKYLDFDIAFLTVGPMDPRGYFNYGLANSVTSAVVT
                     KAKKIVVEVNEHVPYCLGGNQESIHISRVDHIVEGKNLPLPQIPALEPTDIDKQIAKH
                     LLEQIEDGACLQLGIGGLPNVVGKMIAESDLKDLGVHTEMLVDSYVDLYHAGRVTGAK
                     KNIDKYKMSYTFAMGTNKLYDFLHHNPTCASYPVNYINDPRIIALNDKVVAVNNAIAV
                     DLFSQVCSESAGIAHKSGTGGQLDFIFGAFGSHGGKGFICLSSTYKDKDGNLVSRIVP
                     TLPPGSIVTVPRSIVQYVVTEYGAVQLKGKSTWERAEALISIAHPDFRDELIKKADEM
                     NIWTHTNKIE"
     gene            complement(1245..>2003)
                     /locus_tag="PK348_09060"
     CDS             complement(1245..>2003)
                     /locus_tag="PK348_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002685639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HXK66403.1"
                     /translation="NLLQKPVIDHWWQTETGWPIAANCVGIELLPIKAGSPTKPVPGY
                     QVHIINDEGKQLPNGTEGIVVIKLPLPPGTLPTLWQNDDKFRESYLDIYDGYYFTGDG
                     GYYDEDGYLFIMGRIDDVINVAGHRLSTGAMEEVISKHPDVAECAVIGVEDSFKGQLP
                     VGFVVLKEGTTKSHDQIKNDLAQMVREQIGAVACYKTTLIVQRLPKTRSGKILRGTMR
                     KIADKKPYTTPSTIDDPAILDEIKDALSKITVNA"
BASE COUNT          605 a          381 c          414 g          603 t
ORIGIN      
        1 aaaacgcgtt gaggaattac gcaatgtaga tgttagaagc gtttgttata tgcgcatacc
       61 caaaatagtt gaagctgatc ccgagcagaa acatttcatt ttgaatgact tccattttgg
      121 aactatctca cgaagattgt atgatcagca tctttgtaat tatattccaa ttacctatca
      181 tcagggccca cgggtaatca gaaaatactt agattttgat atagcatttt taactgttgg
      241 cccaatggac ccacgaggtt atttcaatta tggccttgca aattcagtaa cgtctgcagt
      301 tgtaacaaaa gcaaaaaaga tagtcgtaga agttaatgaa catgtcccat actgtttggg
      361 aggaaaccag gaatcaattc atatatcacg ggtggaccat atagttgaag gcaaaaacct
      421 gccgctacca caaatacctg cactggagcc aactgacatt gataagcaaa ttgcaaaaca
      481 tcttctggaa cagattgaag atggagcatg cctgcaactg ggtattggtg ggttgcccaa
      541 tgtagttggg aaaatgattg ctgaaagtga cctcaaggat ttaggagttc atactgagat
      601 gcttgttgac tcatatgttg acttatacca tgcaggaaga gtaacaggtg caaaaaagaa
      661 tattgataaa tacaaaatga gctatacttt tgcaatgggt accaacaaat tatatgactt
      721 cctgcatcac aacccaacat gtgcatcata tcctgttaat tatataaatg acccaagaat
      781 aatagctctc aatgataaag tggttgcagt taataatgct attgctgttg atttattcag
      841 tcaggtttgt tcagagtcag ctgggatagc tcataaatct ggtactggcg ggcagcttga
      901 ttttattttt ggtgcatttg gttctcatgg tgggaaagga tttatttgcc tgtcttcaac
      961 atataaggat aaagatggga atttggtatc aaggattgtg cccacattgc caccaggttc
     1021 tattgttaca gtccctcggt ctattgtaca gtatgtagtg acagaatatg gtgcagtaca
     1081 actcaaggga aaatccacat gggaaagggc agaggcattg atcagcattg ctcatccaga
     1141 cttcagggat gaattaatca agaaagctga tgaaatgaat atatggacac atactaataa
     1201 aattgaataa aaaaacgccg gtgttaaacc ggcgttttta tctactatgc gttaactgta
     1261 atctttgata gtgcatcctt tatttcatcc agtatagccg gatcatcaat agtagatggg
     1321 gttgtatatg gctttttatc agcaattttt cgcatggtgc cacgcagaat ttttccggaa
     1381 cgggttttgg gtaaacgctg aactatcagt gttgttttat aacaggcaac agcaccaatc
     1441 tgctcacgaa ccatctgtgc cagatcattc ttaatctgat catgactttt agttgtgcct
     1501 tctttcaata caacaaatcc aacaggtaat tggcctttaa agctatcttc cactccaatg
     1561 acagcacact cagcaacatc tggatgtttt gaaataactt cttccatggc acctgttgaa
     1621 agtctgtggc cagcaacatt gataacgtca tcaatcctcc ccattataaa cagatatccg
     1681 tcttcatcat agtatccgcc atcgccggtg aagtaatatc catcatatat gtccagatat
     1741 gactcacgga atttatcgtc attctgccat aatgttggaa gtgtcccggg aggaagtgga
     1801 agttttatta cgacaatacc ttcagttcca ttgggcaact gtttgccttc atcgttgata
     1861 atatgtacct gatagcctgg aactggtttg gttggggatc ctgctttgat tggtagcaat
     1921 tctatgccaa cacaatttgc agcaataggc caaccggttt cagtttgcca ccagtgatca
     1981 atgactggtt tttgtaatag att
//
LOCUS       DAOSZP010000441         1996 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_42719_length_1996_cov_3.822772, whole genome shotgun sequence.
ACCESSION   DAOSZP010000441 DAOSZP010000000
VERSION     DAOSZP010000441.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1996
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42719_length_1996_cov_3.822772"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            84..1670
                     /gene="cimA"
                     /locus_tag="PK348_09065"
     CDS             84..1670
                     /gene="cimA"
                     /locus_tag="PK348_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HXK66404.1"
                     /translation="MDTDKKHIILYDTTLRDGAQTFGISFSLQDKIRIALELDKLKID
                     YIEGGWPGSNPKDNIFFSEIRKANLKHSKIAAFGSTKRPKMKLTEDSLLQDLINSSAP
                     TLTIVAKSWDFHVTDALNISLEENLALVYDTIAYLKDKGFEVFLDAEHFYDGYKANPE
                     YAMKVLESAVKAGVDMLVLCDTNGGTLTHEISSITGTVCSTYKTPVGVHHHNDAGVAV
                     ANGIAAVLSGAISVQGTINGYGERCGNCDLTALIPNLVLKLGCSCNAAESLYHLTETS
                     RYISEIANLAHNERAPYVGDNAFAHKGGIHVSALKRNTSTYEHINPELVGNRRKVLIS
                     ELAGKSNIQVKAKELGISINNIELPEKILRRIKDLEDQGFQFEAAEGSFELLIRRSNG
                     EYAPFFTLKGFRVITEKDESGRVTCEATIKVEVDGQIEHTAANGNGPVQALDNALRKA
                     LENFYPEIRELQLSDYKVRVLNEKDGTGSSVRVLIDQKRHTEHWGTVGVSTNIIEASW
                     QALVDGIEYMLLKTRKNTSH"
     gene            1696..>1996
                     /locus_tag="PK348_09070"
     CDS             1696..>1996
                     /locus_tag="PK348_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012773972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HXK66405.1"
                     /translation="MDSRPIGIFDSGIGGLTVCKAINKLLPGENLIYFGDTARFPYGT
                     RSAETLIRYARQITSYLLSRNVKMIVIACNTSSAAALPTLQQENTIPIIGVINAGA"
BASE COUNT          633 a          394 c          403 g          566 t
ORIGIN      
        1 attatttaat aatatttgta gtatagtgta aaatgtaata tatttatctt catactatat
       61 atcataattt tttatagcta tatatggata ctgacaaaaa acacattata ctctatgaca
      121 ccacattacg agatggtgca caaacatttg ggatatcttt ttcccttcag gataaaatac
      181 ggattgcact ggaattagat aagcttaaaa tagattatat tgaaggcggt tggccaggat
      241 ctaatccaaa agacaatata tttttttcag agatccgtaa agccaacctg aaacattcaa
      301 aaattgctgc ttttggatca acaaaacgtc caaaaatgaa attaactgag gactctctgt
      361 tacaggacct tattaattcc agcgctccta cattgactat tgttgcaaaa tcctgggatt
      421 ttcacgttac tgatgccctt aacataagcc ttgaagagaa tttagctctt gtatatgata
      481 ccattgcata tttaaaggat aaaggctttg aagtattcct ggatgccgaa cacttttatg
      541 atggttataa agctaaccct gaatacgcca tgaaagttct ggaaagtgct gtaaaagcag
      601 gcgttgatat gcttgtactg tgcgacacca atggcggcac actgacccat gaaatcagct
      661 ctataacagg aactgtatgt tctacataca aaactcctgt tggcgttcac caccacaatg
      721 atgcaggggt ggctgtggca aatggcatag cagccgtgtt aagcggtgcc atttcagtac
      781 agggaaccat caacgggtat ggtgaacgct gtggcaactg cgacctgact gccttaatcc
      841 ccaatcttgt attaaaatta ggttgtagtt gcaatgctgc tgaatcattg taccatttaa
      901 ctgaaactag ccgttatatc agtgaaattg caaatttagc acataatgaa cgcgctccat
      961 acgtgggtga taatgcattt gctcataaag gcggtatcca tgtatcagca cttaagcgca
     1021 atacatccac ttatgaacat ataaaccctg aacttgtagg caacaggcga aaggttctta
     1081 tttcagagct ggcaggtaaa agtaacatcc aggtaaaagc taaagaacta ggtatttcta
     1141 ttaataacat tgaattgccc gaaaaaatac tacgacgcat caaagactta gaagaccagg
     1201 gatttcagtt tgaagcagca gaaggatcgt ttgaattatt aatcaggcga agcaatggcg
     1261 aatatgcacc attttttacg ctcaagggtt tcagggtaat tactgaaaag gatgaatcag
     1321 gcagggtaac ctgtgaagct accataaaag tagaagtaga tggtcaaatt gagcatactg
     1381 ctgcaaatgg gaacggccct gttcaggctc tggataacgc attgcgcaag gcactggaaa
     1441 acttttatcc agaaatccgt gaattacagc tttctgacta taaggtacgt gtactcaatg
     1501 aaaaagatgg caccggttct tcggtccgcg tccttattga ccagaaacgc cataccgaac
     1561 actggggcac cgtaggagtc tctaccaata ttattgaagc ttcatggcag gctctggttg
     1621 atggtataga atatatgtta ctgaaaactc gaaagaacac atcacattaa tatacaacag
     1681 gcataatcaa ctgttatgga ttcacgaccc attggcatat ttgactctgg tattgggggg
     1741 cttactgtat gtaaagctat taataagctt ttgcccggag aaaatcttat ctattttggt
     1801 gatacagccc gtttccccta tggtacacgc tctgcagaaa cccttattcg gtatgcacgt
     1861 cagattactt catatctgct atcacgcaat gttaaaatga ttgtgatagc atgcaacaca
     1921 tcatcagcag cagcccttcc tactctgcag caggagaata ctattcccat tattggcgtt
     1981 attaatgcag gtgccc
//
LOCUS       DAOSZP010000442         1986 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_43005_length_1986_cov_3.872087, whole genome shotgun sequence.
ACCESSION   DAOSZP010000442 DAOSZP010000000
VERSION     DAOSZP010000442.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1986)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1986)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1986
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43005_length_1986_cov_3.872087"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            89..613
                     /locus_tag="PK348_09075"
     CDS             89..613
                     /locus_tag="PK348_09075"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HXK66406.1"
                     /translation="MFDSKRVPLSNRQRSVMEKKYPYYGAATLMDYVDNYIFDGVYVL
                     LGEDGTVMDEKGYPILQYVWGKFWVNNHAHVLKGKGVFSDEYLYILLKNTNVASIVTG
                     AVQLKINQNNLTGLKIMIPPEDVVKQYNDVVKDIFACFRSNADEIKILISLRDTLLPK
                     LISGEIRVPVEEVV"
     gene            643..>1986
                     /locus_tag="PK348_09080"
     CDS             643..>1986
                     /locus_tag="PK348_09080"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019812 - type I site-specific
                     deoxyribonuclease complex [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HXK66407.1"
                     /translation="MPSHNIYEDELEQAALEWFEELGYETAFAPDISPGGDYPERSDY
                     SDIILEERLKDALKRINPDLPQEALDDALHQILVPLNPALIDNNHLFQKMVTDGVNAT
                     FRANDGRIVSKQVRVFDFNEVKNNDFLAVNQFTVIENRVEKRPDVVVFVNGIPLVVIE
                     LKNLANEDVEISDAYNQIQNYISTIPSLFTYNAFAVISDGVNARAGTITADEDRFMMW
                     RTIDGDEIAPLSRPQLEVLIKGMFEKERLLDIIKSFILFQTDGKDTYKILAGYHQYHA
                     VNKAVESTIRAAITEGDRKIGVVWHTQGSGKSLSMVFYVGKLVLSKELNNPTIVVITD
                     RNDLDDQLFSTFAKSKDLLRQEPVQAQDRADLRKLLTRESGGIIFTTIHKFAPEEKGD
                     SVPVLTDRENVIVIADEAHRSQYGFRAEIVKDDTEADVKYGYAKYMRDALPNASYI"
BASE COUNT          630 a          336 c          443 g          577 t
ORIGIN      
        1 aaccgtttaa gaacggagag tttgaggaaa gcgaacttgg gcttatacca aagggtggag
       61 agttggagtt ttaggagata ttatagatat ttttgattca aaaagagttc ccctttctaa
      121 tcggcaaaga agtgttatgg aaaagaaata tccatactat ggagctgcta cattgatgga
      181 ctatgttgat aactatattt ttgatggtgt ctatgtttta cttggtgagg acggcacagt
      241 gatggatgaa aaaggatacc cgatactgca atatgtctgg ggtaaatttt gggttaataa
      301 ccatgcgcat gttctgaaag gtaaaggtgt attttcagat gaatacttat atatacttct
      361 taaaaatacg aatgtagcaa gtatagttac aggtgctgtt cagttaaaaa ttaaccaaaa
      421 taatttaact ggattaaaaa ttatgattcc tccagaagat gtagtgaagc aatataatga
      481 tgtagtcaaa gatattttcg cttgttttcg ttccaatgct gatgaaatta aaatattgat
      541 atcattacga gacactcttt tacccaaact aattagcggt gaaataagag taccagtgga
      601 agaggttgta tgaactgatt gtgatagaaa gggggaaacc ttatgcctag ccataatata
      661 tatgaggatg aattagaaca ggctgcattg gaatggtttg aagaacttgg ttatgaaacc
      721 gcttttgcac ctgatatttc gcctggtggg gattatcctg aacgttccga ttattcagat
      781 attattctgg aagaacgcct taaggatgcc ttaaagcgga taaatcctga tttaccccag
      841 gaagctcttg atgatgccct acatcaaata cttgtaccgc tcaatcctgc cctcatagac
      901 aataatcatc tttttcagaa gatggttacc gatggagtga atgctacttt tagagctaat
      961 gatggcagga tagtcagcaa gcaggtcagg gtttttgact ttaatgaagt taaaaataat
     1021 gattttctgg ctgttaacca gttcactgtg attgaaaaca gagttgaaaa gcgtcctgat
     1081 gtggtggtat ttgtcaacgg tattcccctt gtagttatcg agcttaaaaa cctggcgaat
     1141 gaagatgtgg aaatatctga tgcatataat caaatccaga attatataag caccattcca
     1201 tccctattta cttataatgc ttttgcagtt atatccgatg gagttaatgc aagagcaggc
     1261 acaatcactg ctgatgaaga caggttcatg atgtggcgta caattgatgg ggacgaaata
     1321 gcaccattaa gccgcccaca gcttgaagtc ctgatcaaag gcatgtttga aaaggaacgc
     1381 ctgcttgata tcattaagag ctttatactg ttccagactg acggaaagga cacatataaa
     1441 attcttgccg gctatcatca atatcatgca gttaacaagg cggtcgaaag cactataaga
     1501 gctgccatca ctgaagggga tcgtaaaatc ggtgttgttt ggcatactca gggaagcggt
     1561 aaaagtcttt ctatggtctt ctatgttggc aagcttgttt tgtcaaaaga acttaacaac
     1621 cctacaatcg tagtgattac cgaccgtaac gaccttgacg atcaactgtt ttccaccttt
     1681 gcaaaatcaa aagatttatt acgtcaggaa ccggttcagg ctcaggacag ggcggatctt
     1741 cgcaaactcc tgacaaggga atcgggaggt attatcttta caaccataca caagtttgct
     1801 cctgaagaaa aaggagacag cgtaccggtt ttaacagacc gggaaaatgt aattgttatc
     1861 gctgatgaag cgcatcgaag ccaatacggg ttcagggcgg aaattgttaa ggatgataca
     1921 gaagctgatg ttaaatacgg ttatgcaaaa tatatgcgtg atgcccttcc aaatgcgtct
     1981 tacatt
//
LOCUS       DAOSZP010000443         1978 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_43205_length_1978_cov_3.689028, whole genome shotgun sequence.
ACCESSION   DAOSZP010000443 DAOSZP010000000
VERSION     DAOSZP010000443.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1978
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43205_length_1978_cov_3.689028"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1607
                     /locus_tag="PK348_09085"
     CDS             <1..1607
                     /locus_tag="PK348_09085"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HXK66408.1"
                     /translation="LLAEYIPLATSFLTFLLAIIALYFYFKNKDVLTLYFAIVSLLWC
                     ISAMHFYLPHYWIMDFNTQDKIYYALTAVIAIVVYFFFENVTDSLNRTLRIIITIAAA
                     CMIILSLSATEHDPVTGWRFQVIGSFGLIVQILWGALIIKGIRNNKKEAQTLLIPYIF
                     MMICVGHDSLAVSGIFYTNFFWINLGYPALIIGIGAILSQRSAKLAQELLESKQYIEK
                     KNADLIQIVQKINQSIGELQHVAKEVESSARTLQNSMHDQTTSIEQTSAALEEFSTTV
                     DIIASNSKHQDTILNKNRDLLMELIKGIGHITDAAKTAVKLSYKSQGQTAVTKEHLSE
                     ALKGIEKIKNYSDTILTITETINDIAEKTNLLSLNASIEAARAGEYGRGFAVVADEIG
                     KLADRSLEQSKNIQAILSDIVTDIENQTILMGTVVYSNEDVERSVYMVNHAIDTILDF
                     CISQEQLTKSIEENMALVLQGSSQITSATQEEKITIGEISSTIQQLVTITNAVYENTE
                     MLAKTLTKILSQIDMLQSVVQTKNNS"
     gene            1625..>1978
                     /locus_tag="PK348_09090"
     CDS             1625..>1978
                     /locus_tag="PK348_09090"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HXK66409.1"
                     /translation="MNYRHYKKTTLLFFILLVAIFLSLIIFFKAFYPCPLSTIPSKTT
                     EHDFLQYFDHRIKVLMGKYEIPGVNIAIIRQGKILWANAYGFADKEKTIPMKLETTCR
                     VESISKSVTAWGILKL"
BASE COUNT          696 a          360 c          344 g          578 t
ORIGIN      
        1 cattacttgc agaatatata ccacttgcaa ccagcttcct tacattcctg ttagctatta
       61 ttgccctgta cttttatttt aaaaacaaag atgtgcttac attatatttt gcaattgttt
      121 ccctcctgtg gtgtataagt gcaatgcatt tttatttgcc acattactgg attatggact
      181 ttaatacaca ggataaaata tactatgcgc tgactgcagt tattgcgata gttgtatact
      241 tcttctttga aaatgtgaca gatagcttga acaggacact caggattatt attacaattg
      301 ccgctgcatg tatgataatt ttaagccttt ctgctactga acatgatccc gtaacaggct
      361 ggcgattcca ggttattgga tcatttggac ttattgttca aatactgtgg ggagcactta
      421 ttataaaggg tatacgaaat aataaaaaag aagcacaaac tctattaatc ccttatattt
      481 ttatgatgat ctgtgtgggc catgattcac ttgcagtatc agggattttt tataccaact
      541 ttttctggat aaatttgggg taccccgcat taattatagg cattggcgca atattgtcac
      601 aacgctctgc aaaactggca caggaactgc ttgaatcaaa acaatatatt gaaaagaaga
      661 atgctgacct gatacagata gtacaaaaaa tcaatcagtc aattggggaa ttacagcatg
      721 tagcaaagga agttgaaagt tctgcacgta ctttgcaaaa tagcatgcat gatcagacca
      781 ccagcattga acaaacatca gcagccctgg aagaattttc aaccacggta gacatcattg
      841 cttctaattc caagcatcag gatacaattc ttaataaaaa tagagacctg ttaatggaat
      901 taattaaagg tattggccat attaccgatg cagctaaaac agcagtcaaa ctgagctata
      961 aaagtcaggg gcaaacagct gtaacaaaag aacacctttc tgaagcactt aaaggcatag
     1021 aaaaaataaa aaactattcg gacaccatat taacaataac cgaaaccatt aatgatatag
     1081 ctgaaaaaac caatctgtta tcacttaacg cttcaattga agctgcacgg gcaggcgagt
     1141 atgggcgcgg ttttgccgtt gtagctgatg aaattgggaa attagcagat agatccttag
     1201 aacaatcaaa aaatatccag gcaatcttat cggacatagt tactgatatt gaaaaccaga
     1261 caatccttat gggcacagtt gtatactcaa atgaagatgt tgaacgctct gtttatatgg
     1321 ttaatcatgc aatagacaca atccttgatt tttgcatatc acaggagcaa ttaacaaaaa
     1381 gcatcgaaga aaatatggca cttgtattgc agggttcttc gcagattaca agcgccacac
     1441 aggaagaaaa aataactatt ggagagatta gttcaacaat acagcagctt gttaccatta
     1501 caaatgccgt atatgaaaat accgaaatgt tagcaaaaac cctcacaaaa atcctgtcac
     1561 agattgatat gcttcaatcc gtagtccaaa caaaaaacaa ttcttaagaa aggattatat
     1621 tactatgaac tatcgccatt ataaaaaaac aacactatta ttttttatac tgttagttgc
     1681 aatttttctt tcactgatta ttttttttaa agcattttat ccttgtccct tatccaccat
     1741 accatcaaaa actacagaac atgacttttt acagtacttt gaccatcgta taaaagtttt
     1801 aatgggaaaa tatgaaattc ctggagtaaa tattgctatt atcaggcaag ggaaaatatt
     1861 gtgggcgaat gcatacggct ttgcagacaa agaaaaaaca atacccatga agctggaaac
     1921 aacttgccgc gttgaatcaa tttcaaaatc agttacagcg tggggtatat taaaactg
//
LOCUS       DAOSZP010000444         1977 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_43237_length_1977_cov_3.216441, whole genome shotgun sequence.
ACCESSION   DAOSZP010000444 DAOSZP010000000
VERSION     DAOSZP010000444.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1977
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43237_length_1977_cov_3.216441"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(380..1414)
                     /locus_tag="PK348_09095"
     CDS             complement(380..1414)
                     /locus_tag="PK348_09095"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005495120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HXK66410.1"
                     /translation="MKTRITELFGIKYPIILPGMSWISTPELVAAVCNAGGTGYLATG
                     PLTPEQTRQAIRRIRELTDKPFGAGCTLIMPGARENAEVMLEEKVPIINVSLGKCDWI
                     AERAHKYGGKVIATVVTEKHALAAEKQGADALQVTGHEAAAHGGQVTTFVLVPAIVDK
                     VKIPVVAVGGIADGRGMAAALALGAEGIGMGTRLSMTKESPLHKKTIEAQLKAGIEDT
                     IYSNRFDGIYCRVLKTKSAEKAIRKGMSLPHAALESYKIAKMMNMPYFKLALGVMLQG
                     PRMMIRLAHFATAFDKIKVATEKGDLDYGVQLIGQCQGLIDDVPTVKEVIERTVKEAK
                     AIYQKNMKKF"
     gene            complement(1532..>1977)
                     /locus_tag="PK348_09100"
     CDS             complement(1532..>1977)
                     /locus_tag="PK348_09100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HXK66411.1"
                     /translation="KLYIRITDRIKDLIITAGGKNIAPQVIETMLGEDLYIEQVVVVG
                     DGRKYISALIVPNFEILGEYAKSKGIQYSSRQELISNPAIIEFYNNKIEKLTESLGQV
                     EKIKKFTLMPHEFTQENGEITPTMKIRRKVIQQRYNDIIDKMYLE"
BASE COUNT          597 a          430 c          286 g          664 t
ORIGIN      
        1 aaaacctcat gattaaagta cttatcaata caatcacgta gatgatgata aacctttaat
       61 ccagaacgag aagcaggatt gactataata gtggcttttg gtataacatc atttttattg
      121 gaagccatat actgaaatcc atgttaagga gttacattca attcaatgtg attatcaagc
      181 agaatattaa aataatttta aaaacattta ctatatagat aaatattttc aatatatttt
      241 taattaatat ggcaatcaca tcaaaacttt ttatattacc atcaaaataa ttgtatcatg
      301 tcataaaata ttcataaaac caatatataa aaaagcagag actttcatct ctgctttttt
      361 aataattatt ttttataact taaaattttt tcatattctt ctgatatata gcttttgctt
      421 cttttactgt tctttcaata acttctttta cggttggcac atcatctatt aacccctgac
      481 actgcccaat aagctgcaca ccataatcaa gatcgccttt ttcagttgct acctttattt
      541 tatcaaatgc agtggcaaaa tgtgccagcc gaatcatcat tcttggcccc tgcagcataa
      601 cgcctaacgc taatttaaaa taaggcatat tcatcatctt agctatctta tatgattcta
      661 aggctgcatg aggcaaactc atgccttttc ttatagcttt ttcagctgat ttggttttta
      721 acacacggca gtatattcca tcaaatctat tggaatagat agtatcttca atacctgctt
      781 taagctgtgc ttcaatggtt ttcttatgta atggactttc ctttgtcatt gacaaacggg
      841 tacccattcc aataccttct gcacctaacg ccagtgcagc agccatccca cggccatctg
      901 ctataccgcc aacagcaaca acaggaattt ttaccttatc aactatcgcc ggaacaagaa
      961 caaaggtggt aacctgccca ccatgagcag ctgcctcatg cccggttacc tgcagagcat
     1021 cagcaccctg tttttctgct gccagagcat gtttttcagt aacgacagtt gcaataactt
     1081 tgccaccata tttatgtgca cgttctgcaa tccagtcaca tttacccaat gatacattaa
     1141 tgattggaac tttttcctca agcatgactt cagcattttc gcgtgcacct ggcataatga
     1201 gggtacatcc tgcaccaaaa ggtttatcgg taagctcacg tatccttctg atagcctgtc
     1261 gtgtttgctc cggtgtcaat ggacctgttg ccagatagcc tgtaccgcct gcattacata
     1321 cagcagctac cagttcagga gtgctaatcc agctcatccc gggaaggatg attggatatt
     1381 taattccaaa cagttcagta attcttgtct tcatacaata actccccatt tactaaaaat
     1441 tattattctc attacctaaa aacataacac cgttatacta ctattgtagc aataaaaatt
     1501 ttatatacaa aaaatactaa tttaaaccac attattccag atacatctta tcaatgatgt
     1561 cattgtatcg ttgctgtatt actttgcgtc taattttcat tgttggtgta atctcaccat
     1621 tttcttgtgt gaattcatgt ggcatcaagg taaacttttt aatcttctca acctgtccca
     1681 agctttcagt tagtttttca atcttattgt tgtagaattc aataattgcc ggatttgaaa
     1741 tcaattcctg ccgtgaagaa tactgaattc cttttgattt tgcatattca cccaagattt
     1801 caaaattggg cacaatcagg gcactgatat attttcgccc atcaccaacc accacaacct
     1861 gctcaatgta gagatcttcg cctaacattg tttcaattac ctgcggagca atattcttac
     1921 caccagcagt tataatgagg tccttaatcc tgtcagtgat gcgtatatac aactttc
//
LOCUS       DAOSZP010000445         1977 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_43239_length_1977_cov_3.034339, whole genome shotgun sequence.
ACCESSION   DAOSZP010000445 DAOSZP010000000
VERSION     DAOSZP010000445.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1977
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43239_length_1977_cov_3.034339"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            103..933
                     /locus_tag="PK348_09105"
     CDS             103..933
                     /locus_tag="PK348_09105"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007782582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HXK66412.1"
                     /translation="MNEIKTVLDEVVSALADVSGIQAVVLGGSRARGTHSPDSDIDIG
                     IYYNTETLDLVSLNKAAKLVDDEHRDNLVVPPGAWGEWVNGGGWLMMDGYHVDFILRD
                     IERVENVISECQEGIISAHYQTGHPHAYMNVMYMGELAVCKVLWDKQGNISALKQVAK
                     KYPPKLKRAIIGFFTFEAEFSLMFAENNARKNDVYYVTAHIVRAISALNQVLFAVNEE
                     YCLNEKKAVGMIEHFNIHPLGYKDKINSIFAAAGTDGANACVQLKQLINEAKGIFLND
                     "
     gene            981..1712
                     /locus_tag="PK348_09110"
     CDS             981..1712
                     /locus_tag="PK348_09110"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011838189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXK66413.1"
                     /translation="MNNIYDNRQFFDQYSKMSRSQQGLSGAGEWHQFKALFPELSYKS
                     VLDLGCGYGWHCKYAVECGAKQVLGIDLSEKMIHEAKAKNADSKITYRVCGLEEYDYP
                     ANSYDCVVSNLVLHYIADIDTIFRKIYLTLKADGVFLLNIEHPVFTAGVNQDWIYDSN
                     GRSQHWPVDDYFYPGERVAHFLGLDVTKQHHTLTQILMGLIKAGFRLDVVEEAMPSAD
                     MMDIPGMSDEMRRPMMLLIKSVKQA"
     gene            1771..>1977
                     /locus_tag="PK348_09115"
     CDS             1771..>1977
                     /locus_tag="PK348_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_047211550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HXK66414.1"
                     /translation="MQYGPDPSSIYPNENIKSICYIKNLITRPNIIVGDYTYYDDING
                     AERFEEHVTHHYEFIGDKLIIGKFC"
BASE COUNT          610 a          339 c          478 g          550 t
ORIGIN      
        1 aggttagatg ttttttcgga agtttttgag gttgtgtggt cggattggat gtgggcatta
       61 acaagctatt ttaaaaagat cgctttaatg aggtgagcat acatgaatga aattaaaaca
      121 gtattagatg aagttgtatc agcattggca gatgtgtcag gcatccaagc agttgtgctt
      181 ggcggctctc gcgcaagggg cacgcattcc cctgactcag atattgatat cggtatttat
      241 tacaatacgg agacacttga ccttgtgtcc ttgaacaagg cagccaaact tgtggatgat
      301 gaacacaggg ataatttggt cgttcctcct ggtgcatggg gagaatgggt taatggcggt
      361 ggctggctta tgatggatgg ataccatgtt gactttatac tacgggatat agaacgtgtg
      421 gaaaatgtaa tttcagaatg tcaggaaggg attatttccg cacattatca aacaggacat
      481 ccccatgcct acatgaatgt gatgtatatg ggagaattag cagtatgcaa ggtattgtgg
      541 gataaacagg gtaatatttc agctttgaag caagtcgcga aaaaataccc gccgaaattg
      601 aaaagagcta ttattggttt ctttacgttt gaagcagagt tttctttaat gttcgcggaa
      661 aacaatgcca ggaaaaacga tgtttattat gttactgctc atattgtacg ggcgatttca
      721 gcgctgaacc aggtgttatt tgcagtaaat gaagagtact gcctgaacga aaaaaaggca
      781 gtcggcatga tagagcattt taacattcac ccgcttggat acaaagataa aataaattca
      841 atttttgcgg cagccggaac agatggagca aatgcctgtg tgcagctaaa gcaattgatt
      901 aacgaagcga aaggtatttt tctgaatgac tgatgaacat atccggtgaa agattctaac
      961 tacacaaaga aggtgagaat atgaacaaca tttatgataa tagacaattt tttgaccagt
     1021 attcgaagat gtcacgcagt caacaaggct tatccggagc aggtgaatgg catcagttca
     1081 aggccttgtt tcctgaatta agctacaaaa gcgtactcga tcttggatgc ggttatggct
     1141 ggcattgcaa atatgcggtt gagtgcggtg caaagcaggt attgggtatt gacctgagtg
     1201 aaaaaatgat acatgaagca aaagcaaaaa acgcagactc gaaaattacc tatcgggttt
     1261 gcgggctgga ggaatatgat tatcccgcca attcatatga ttgcgtggtt tctaatctgg
     1321 tgcttcatta tattgcagat attgatacga ttttcaggaa aatatacttg acgctaaagg
     1381 ctgatggcgt tttccttctt aacattgaac atcctgtttt tacagcgggt gtaaaccagg
     1441 actggattta tgattcgaat ggcagatcac agcactggcc tgttgatgat tatttttatc
     1501 ccggagagcg agttgcccat ttcctaggac tggatgtcac gaaacaacat catacattaa
     1561 cgcaaattct aatggggctt ataaaagccg gatttcggct ggacgttgtt gaggaagcaa
     1621 tgccaagcgc cgatatgatg gacattccgg gcatgtcaga tgaaatgcgc cgcccaatga
     1681 tgctgctaat taagtctgtg aaacaagctt gatagcatct gagttgggaa agcgttgata
     1741 gcggaaagcc aacgcgcgag gaggtttaat atgcaatatg gaccagatcc aagttccatt
     1801 tatccaaatg aaaacatcaa aagcatatgc tacattaaaa atttaatcac acgtccaaac
     1861 attatagtag gcgattacac ctactatgat gatatcaatg gtgcggaacg atttgaagag
     1921 cacgttacac atcactatga atttataggt gataagctca tcatcgggaa attctgc
//
LOCUS       DAOSZP010000446         1961 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_43710_length_1961_cov_2.645331, whole genome shotgun sequence.
ACCESSION   DAOSZP010000446 DAOSZP010000000
VERSION     DAOSZP010000446.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1961
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43710_length_1961_cov_2.645331"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..254
                     /locus_tag="PK348_09120"
     CDS             <1..254
                     /locus_tag="PK348_09120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66415.1"
                     /translation="QQVLEELYAFLGTDLIANPDSRLWDDSAELLVAFASMMNDLNES
                     RYYKSTFEDLTKPAYTSPAEYLYSDEFDPYSGLGQVLTK"
     gene            401..1951
                     /locus_tag="PK348_09125"
     CDS             401..1951
                     /locus_tag="PK348_09125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66416.1"
                     /translation="MMYRLPGKFVVIICAIVTVQGLAYANFADTYGFSAIGISRGNAM
                     TAVVNDWSSVYYNIAGLGRTRGIMPVPSGTDKQQLTLQQKGAKEAQNQPTEMSVSPYD
                     IFPYKDQLAINYMYTQPQMTIDIPRTDIVADKELNFGTVTLGLVLDLNHFVKMPSFIS
                     SSRFGLGLGVMQDGSLVKVYDIDLRTHDFVRYGKEAERTVILAGVGFGFMDDLFGIGA
                     GANVWSGGRGAVNLTEVELGPNEQTPNSEVQMELTPKIAPVAGLYISPGKKIKALQGL
                     DIGVAYRGEVYMEVDPFATTAELYTAGVTLEMALSIYDYYTPHIISGGIAYTFLPIKP
                     LSGLTLSVDVEYQMWSKCKVSKSKTAYWANKGITIPEFQDIIVPKVGVSYNFGCLSPK
                     MQWLTMNLGYSFRPSYVPDDAVNDASLFNFMDANTHIGSLGLSFTIPKTGPMVAPVVI
                     TLAGQMQMLQKRQVKKDRVAIEKIYENFDGNPSDDDVTQAEIDALYPDYSYSGFVYSG
                     FVEVALRW"
BASE COUNT          597 a          316 c          465 g          583 t
ORIGIN      
        1 cgcagcaggt gcttgaagag ctatatgcat tcctgggaac tgatttaata gcaaatcctg
       61 attcacggct gtgggatgat tcagctgaat tgcttgttgc atttgcaagc atgatgaatg
      121 atctcaatga aagcaggtat tataaatcaa catttgaaga tttaacaaag cctgcataca
      181 cttcgccagc agaatatctg tacagtgatg agtttgatcc atattcaggg cttgggcagg
      241 tattaactaa atgaggacaa aagaaagcag tccttttatt atgctttacg gctgcttaac
      301 cacaataagc ttatgaaagg agctatcgaa caaaaattaa aagtgaaggg tttgggttat
      361 catatacaat tatcatttaa aatatttttg aatggaggtt atgatgtata gattgcctgg
      421 taaatttgta gtaattatat gtgcgatagt tactgtgcaa gggcttgctt atgcaaactt
      481 tgccgatacg tacggtttta gtgctattgg aatttcgcgt ggaaatgcta tgacagcagt
      541 ggttaatgac tggtcaagtg tatattataa tattgcaggc ttagggagaa cacgcgggat
      601 aatgccggtg ccttcaggaa cagacaaaca gcaacttaca ttgcagcaaa aaggagcaaa
      661 agaggcacaa aaccagccaa ctgagatgtc agtatcgccc tatgatattt tcccttataa
      721 agatcaactg gccattaatt atatgtacac tcagccgcaa atgacaatag atattccccg
      781 caccgacata gttgctgata aggagttgaa ttttggtact gtaaccctgg gactggtgct
      841 ggatttaaat cattttgtaa aaatgccgtc atttatttca tcaagccgct ttggattggg
      901 actgggtgtt atgcaggatg gctcactggt aaaggtatac gatattgacc tgcgtactca
      961 tgattttgtc cgttacggaa aagaagcaga acgaacagtt attttagccg gcgttggatt
     1021 tggctttatg gatgatttgt ttggtattgg agctggtgca aatgtgtgga gcggaggaag
     1081 aggggcggtt aatttaactg aagttgaact gggaccaaat gaacaaacac caaattctga
     1141 agtacagatg gaattaactc caaaaattgc acctgtggcg ggactatata tatcaccagg
     1201 gaaaaagatt aaggctttac aggggcttga tattggtgtt gcctaccgcg gagaggtgta
     1261 tatggaggtt gatccttttg ccactacagc tgagctgtat actgcaggag taactctgga
     1321 gatggcattg agcatttatg attattatac gcctcatatt atttcgggcg gtattgctta
     1381 tacgttttta cccattaagc cgttatcggg gcttacactc agtgttgatg ttgaatacca
     1441 gatgtggtca aaatgtaaag tatctaaatc aaaaacagca tactgggcaa ataagggcat
     1501 tacaattcct gaatttcagg atatcattgt gccaaaagtt ggtgtaagct ataattttgg
     1561 gtgcctgtcg ccaaaaatgc agtggttaac aatgaatctg ggatattcat tcaggccaag
     1621 ctatgttccg gatgatgcag ttaatgacgc gtcattattc aatttcatgg atgctaacac
     1681 acatattggt tcactgggat tgagttttac aataccaaaa acaggaccaa tggttgctcc
     1741 agttgtcatt acattagcag gacagatgca gatgttgcag aaacgtcagg ttaagaagga
     1801 tagagtggca attgaaaaaa tatatgaaaa ttttgatggt aatccatcag atgatgatgt
     1861 tactcaggct gaaattgatg cattatatcc ggattattca tatagcgggt ttgtttacag
     1921 tggttttgtg gaggtagcat tgcgctggta aaatatatat a
//
LOCUS       DAOSZP010000447         1960 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_43734_length_1960_cov_2.791601, whole genome shotgun sequence.
ACCESSION   DAOSZP010000447 DAOSZP010000000
VERSION     DAOSZP010000447.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1960)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1960)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1960
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43734_length_1960_cov_2.791601"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..941)
                     /locus_tag="PK348_09130"
     CDS             complement(<1..941)
                     /locus_tag="PK348_09130"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HXK66417.1"
                     /translation="MKKSAIFIVVLFYVYELSCTTPEERIPKHYIATSSDYSMLFIGD
                     MMFEWAVKDTMQRYGTEYPLKRIKPFFQSFDFVMANLESPLTRSSQSLVYQKYIFKAP
                     PSIAFILAEAGITAVTLANNHMLDYGVTGLLDTLTSLSHAKVLYAGAGNNEITASLPV
                     MRQLGTTRIVVLCYTQIASKEMIAKNNPGINFFELKKAKEDIKKYRFCDCVIINIHWG
                     NEYFYYPSSKQIDIAHALIDAGADAVIGHHPHVYQGIEIYKNKPIVYSLGNFLFGSVH
                     EGINDNIACALYMSGNGKIIKMHIYAIKGIYNDGSIN"
     gene            complement(945..1625)
                     /locus_tag="PK348_09135"
     CDS             complement(945..1625)
                     /locus_tag="PK348_09135"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXK66418.1"
                     /translation="MSDILVAIIVRNNEELLRRTLDSICNCPYAHVMPLIIDDGSFDQ
                     SPSIVSDVDVHFVMHEEPLGYGGALMSAFAYAKDYSLSSLFMLPLEAFADWHTVSGYL
                     SFFQDADIITGNRFTGQTFNEKKQYNDIVQYFNQHAGLNIVDPFSPLKGISMNHADLF
                     EITEFSDAALVQILIQAAHFKLRIKEFECMYPEKNLLSHLDDIENLPGLKDFITGELH
                     LYPYTKGH"
     gene            complement(1618..>1960)
                     /locus_tag="PK348_09140"
     CDS             complement(1618..>1960)
                     /locus_tag="PK348_09140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR00268 family protein"
                     /protein_id="HXK66419.1"
                     /translation="IQGLQHLKIPYSKASNSCLATRIPYGTGITQDILDAIQRAEEFI
                     ISLGFDGVRVRYHFPVARIEVMRQDMPRIVDPEIRNKIVAFCKKLGFLYVTIDLEGFT
                     SGNLNRMLNHE"
BASE COUNT          633 a          384 c          336 g          607 t
ORIGIN      
        1 atattaatac taccatcatt atatatccct ttgattgcat aaatatgcat cttgataatt
       61 ttcccatttc ctgacatata cagtgcacag gctatattat cattaattcc ttcatgaaca
      121 gagccaaaaa gaaaatttcc caatgaataa acaattggtt tatttttata gatttcaatt
      181 ccctgataca catgaggatg atgtccaata acagcatctg caccagcatc aatgagtgca
      241 tgagctatat caatctgttt tgatgaagga taataaaaat attcattacc ccaatggata
      301 tttattatga cacaatcaca gaaacggtat ttcttaatat cctccttcgc tttctttaat
      361 tcaaaaaaat taattccggg attatttttt gcaatcattt cttttgaggc gatctgcgta
      421 tagcataaca caacaatgcg tgttgtcccc aactgacgca taactggcag cgatgctgtt
      481 atttcattgt tccctgctcc agcatatagc acttttgcat gggatagaga agtaagtgta
      541 tccagtaatc ctgtaactcc ataatcaagc atatgattgt ttgccagggt aactgctgta
      601 ataccagctt cagccagaat aaatgcaatt gaaggtggtg ctttaaaaat atatttttga
      661 tatactaaac tctggctact ccgtgtcaat ggtgattcta aatttgccat aacaaaatca
      721 aatgattgga aaaagggttt aattcttttt aatgggtatt ctgtaccata gcgctgcatt
      781 gtgtccttca ctgcccattc aaacatcata tcgccaataa acagcatgct atagtcagaa
      841 gaagttgcta tatagtgctt ggggattcgt tcctcaggtg ttgtacatga tagttcataa
      901 acataaaata atactactat gaaaatagct gactttttca cgttttaatg cccttttgta
      961 tatgggtaaa ggtgaagctc gcctgttata aaatctttaa ggccgggtag attttcaata
     1021 tcatccagat gagatagtaa atttttctca ggatacatac attcaaattc ttttatcctt
     1081 aacttgaaat gagcagcctg tatcagtatt tgcacaagtg cagcatcaga aaattctgtt
     1141 atttcaaata aatcggcatg attcatggaa atgcctttta gcggagaaaa agggtctacg
     1201 atgttcaagc ctgcgtgctg attgaaatac tgtacaatat cgttatattg ctttttttca
     1261 ttaaaagttt gtcctgtgaa tctattcccg gtgatgatat ctgcatcctg gaaaaaagac
     1321 aggtaccccg aaactgtatg ccaatcagca aaagcttcca ggggcagcat aaacaacgaa
     1381 ctcaacgaat aatcctttgc gtatgcaaac gcactcataa gtgccccacc ataccccaga
     1441 ggctcttcat gcataacaaa atgaacatcc acatcagaaa cgattgaggg cgactggtca
     1501 aaagagccat catcaataat aagtggcatg acatgggcat aggggcaatt gcagatagag
     1561 tctaatgtac gacgtaataa ttcctcatta ttgcgaacaa taattgctac aagaatatca
     1621 ctcatgattt aacatcctgt ttaaattacc tgatgtgaat ccttctaaat caatagtcac
     1681 atataaaaat cccaatttct tacaaaaagc aacgatttta tttcgtatct cagggtcaac
     1741 gattcttggc atatcctgtc tcattacttc gatgcgtgcc acaggaaaat ggtaccgtac
     1801 acgtacacca tcaaagccta acgaaataat aaattcctca gccctctgta tagcgtcaag
     1861 tatatcctga gtaatgcctg taccataagg aatgcgtgtg gcaaggcatg aattggatgc
     1921 ctttgaatat gggattttta aatgttgcaa accctgaata
//
LOCUS       DAOSZP010000448         1957 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_43819_length_1957_cov_2.405363, whole genome shotgun sequence.
ACCESSION   DAOSZP010000448 DAOSZP010000000
VERSION     DAOSZP010000448.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1957)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1957)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1957
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43819_length_1957_cov_2.405363"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1876)
                     /locus_tag="PK348_09145"
     CDS             complement(<1..1876)
                     /locus_tag="PK348_09145"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXK66420.1"
                     /translation="MQKAFTIYTAIAQQNKSAIADKLLVLYKYFALFERMQGNYEKAI
                     EWYKKALEHCAQFQCGDSPSRLYIDIAECYRRLEQDSEALVYLELAERSLPQETSVQY
                     KIGWKFAGFGPVYFYDLGQDTVVIGEGKIVSHLSGFEMEILIQNMRADIYAKSGRFGD
                     AATKYLDLVAITKKQKTTLIADAHITALYNAAYCYTRMGDVQKALELYDTALSIAQDS
                     NIPIDKIYQTMVQWAYCYTASHEQKAKQVESLDKAIRTTEQYKKNMYDKVYNSLLDDY
                     TKKMKAQKKKPVDEDIAALKQQAAKQVEAYSIKFSALTALLYMHKASLLEQDITSDKS
                     TDYYGKANEIIGYYSDAERLFSRAISYFGNDASHQEFVIKLLINRGICRIHSGQFRDG
                     YDDIISARFKANKIQSKELVWIAEATLWDLANTYSEVKQLIDNPDRLIDGVLSRVELL
                     PPLYTMKKEFIQKLYDTYADELIQKKKYKEAYAIQQRRQIITLAFSGLSIPKFYKKSD
                     NTIYSKFLRTCFTIQDLEQKLTSAIVNEEEPDVIEKLTSTISYHYKNLTGYRSAASRF
                     LLPYLGVYTPKASEYTIIHFYTFDNKLYGWIVNKNTIDWLLISDSLKPERLTEDLNTF
                     I"
BASE COUNT          568 a          395 c          323 g          671 t
ORIGIN      
        1 gtataaatgt attaagatct tcagtcaatc tttcaggctt cagtgaatca gaaattaata
       61 accaatcaat agtattttta tttactatcc atccatagag tttattatca aatgtgtaga
      121 aatgaattat ggtatattct gaagcttttg gggtataaac tccaagatag ggtaatagaa
      181 atcttgaagc ggccgacctg taaccagtaa gatttttata atgatatgaa atagtagatg
      241 ttagtttttc aataacatct ggctcttctt cattcactat cgcactggtt aatttttgtt
      301 caaggtcctg tatagtgaaa catgtacgca aaaatttgct atagatggta ttatcgcttt
      361 ttttatagaa ttttggtatt gatagtccac tgaatgctag agtgattatt tgcctgcgct
      421 gctgaatggc ataagcttct ttgtatttct ttttctgtat aagttcatca gcatatgtat
      481 catagagttt ctgaataaac tctttcttca ttgtatacag aggcggcagc aattccaccc
      541 gtgataatac cccatcaata agcctatcag gattatctat gagctgcttt acttcactat
      601 atgtgtttgc taaatcccac agcgttgctt cagcaatcca caccagttct tttgattgaa
      661 ttttattagc tttaaatcga gcactaatga tgtcatcata tccatccctg aactgcccag
      721 aatgtattcg gcatattcct ctattaatga gcaatttaat aacaaactcc tgatgagaag
      781 catcgtttcc aaagtaactg attgcccgag aaaataacct ttcagcatct gaataatagc
      841 caataatttc atttgcttta ccataataat cagtactttt atcagatgtt atatcctgtt
      901 ctagaagaga agctttgtgc atgtacagta gtgctgtcaa tgcactaaat ttaatgctgt
      961 atgcctcaac ctgtttagct gcctgctgtt tcaaagctgc aatatcttca tcaactggtt
     1021 ttttcttttg tgctttcatc tttttggtat aatcatcaag aagggaatta tatactttat
     1081 catacatatt ttttttgtac tgctctgttg tccgtattgc cttatcaagg ctttccactt
     1141 gttttgcttt ttgttcgtgg ctggcagtgt aacagtaagc ccattgaacc atggtttgat
     1201 atattttatc aataggaata tttgaatcct gagcaattga tagagcagtg tcatataatt
     1261 ccagtgcctt ctgtacatcc cccatacggg tataacaata tgccgcatta tacaaagcgg
     1321 taatatgcgc atcagctatt aatgtagtct tttgcttttt tgttattgct actaaatcca
     1381 gatatttggt tgcagcatca ccaaacctgc cgctttttgc atagatatct gcacgcatat
     1441 tctggataag tatttccatc tcaaaacctg aaagatggga aacaattttc ccctccccaa
     1501 taacaacggt atcctgtcca aggtcataga agtatacagg gccaaatcca gcaaatttcc
     1561 agccaatttt atactgcact gacgtttcct gcggcaatga ccgttcagca agttccagat
     1621 atacaagcgc ttcagaatcc tgttccaatc tgcgataaca ttcagctatg tctatataaa
     1681 gacgtgatgg gctatcccca cattgaaatt gtgcacaatg ttctaatgct ttcttatacc
     1741 actcaatggc tttttcataa ttaccctgca tccgttcaaa gagagcaaaa tacttgtaga
     1801 gcaccaagag cttatccgca attgcagact tattctgttg agctatcgcc gtataaatag
     1861 taaacgcctt ctgcattgac acatacgccc cactgtaatt cccctgctgc cataaacaat
     1921 agccatagtg atagtaaaaa agagcttctt ctttcaa
//
LOCUS       DAOSZP010000449         1955 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_43863_length_1955_cov_3.402632, whole genome shotgun sequence.
ACCESSION   DAOSZP010000449 DAOSZP010000000
VERSION     DAOSZP010000449.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1955)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1955)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1955
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43863_length_1955_cov_3.402632"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..838
                     /locus_tag="PK348_09150"
     CDS             <1..838
                     /locus_tag="PK348_09150"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HXK66421.1"
                     /translation="IFASITNALKLNIGILTHKERMLYHDYRIHEKIVDLLEAGYPDL
                     VVSDAIDITYGFESAPYPVRLGALIISNNPLAADVVAAYIMNYRPQDVLHLRLAAERG
                     YGSCNIKDIAISGDVDIEKLRKKPKGQSRLFQILQELDTPLKFYSGYAPSTDIICDGG
                     CEAAVKGCLGTIEKRRPGSLKNAKKGAIVTGIYKGDVIIPGGSVLLVGDCTKVEGKLV
                     AKRVLRIKGCPIGARNLFIPVPLLFGLPSPMFDVRDAFLFIINSIQKAFNIVVYRWVL
                     KR"
     gene            complement(938..1585)
                     /locus_tag="PK348_09155"
     CDS             complement(938..1585)
                     /locus_tag="PK348_09155"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HXK66422.1"
                     /translation="MKSNVKSKDYQDFLDDLKGYYEKHDQKEENIPVGTRIRKIREIQ
                     GMDIESLASIAGIDAKYLQDIESLKAFPDLGTIIRLSKALKISTGLIIDEQSGLPYVV
                     IRKNERRKITRKISGKKDHPHYEYISLSSGVISRHMESFIVRLTSNVYYTQDLSAHEG
                     EEFLYVLEGAMKIKLAYKEETLHTGDSIFYHSTIPHTLKSISPKPAVILAVLYTG"
     gene            1766..>1955
                     /locus_tag="PK348_09160"
     CDS             1766..>1955
                     /locus_tag="PK348_09160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66423.1"
                     /translation="MLCHLWAIVTDDGSDTVYSAHAYTEFAYCSIDMCCYERLFPCDN
                     HYCSDTIYPLRYALTLSFK"
BASE COUNT          535 a          365 c          414 g          641 t
ORIGIN      
        1 tatctttgca tcaatcacca atgccttgaa gctaaatatt ggcatactca cgcacaaaga
       61 aaggatgctt taccatgact atcgcatcca tgaaaagata gttgaccttc tggaagctgg
      121 ctatcctgac ctggttgttt cagatgccat tgacattacc tatgggtttg aatcggctcc
      181 gtatcctgta aggctgggag cacttattat ttccaacaat ccactggctg ctgatgtggt
      241 tgcagcgtat atcatgaact atcgccctca ggatgtattg catttgaggc ttgcagctga
      301 gcgtggctat ggttcatgca atatcaagga tattgcaata agtggtgatg ttgatattga
      361 aaagctcagg aaaaaaccca agggtcagag caggttgttt cagatattac aggagcttga
      421 tacgccactc aagttttatt cagggtatgc tcccagtaca gatataatct gcgatggcgg
      481 ctgtgaggct gctgtcaaag gatgtctggg caccattgaa aaacgaaggc ctggttcact
      541 taaaaacgca aagaaaggag ctattgttac gggaatatac aaaggtgatg tgattatccc
      601 cggtggtagt gtattgctgg tgggtgattg tactaaagtt gaagggaagc ttgttgccaa
      661 aagagttttg cgaatcaaag ggtgccccat tggcgcacgc aatctcttta ttcctgtacc
      721 acttttgttt ggactgccaa gcccaatgtt tgatgtgcgc gatgcttttc tttttattat
      781 caattccatt cagaaagcgt ttaacattgt tgtatacagg tgggtactga aacgataaaa
      841 tgatgtgcga tagttactga taattggttc aaatatagtt atggtgttgc ttaaaaaaca
      901 atctagtgca tacggatttt gctgcgaata aaaagtatta tccagtatag agcactgcta
      961 aaatcacagc aggcttgggg ctaatgcttt ttaaggtatg cgggattgtt gagtgataga
     1021 agatgctgtc accagtatgc agtgtttcct ctttataagc taatttaatc ttcattgctc
     1081 cttcaaggac ataaaggaat tcctcgcctt catgtgccga tagatcctgg gtataatata
     1141 cattgctggt taatctgacg ataaatgatt ccatatgccg agaaatcaca ccagaagata
     1201 atgagatgta ttcataatga gggtggtctt ttttacctga gatttttcgg gttatttttc
     1261 gtctttcatt tttacgtata accacatatg gtaggcctga ttgttcatca atgataagtc
     1321 ctgttgatat cttgagcgct ttggataaac gaataattgt tcccagatcg ggaaatgctt
     1381 tgagcgattc aatgtcctgc aaatatttag catcaatacc agctattgag gctaatgatt
     1441 caatatccat tccctgtatt tctctgatct ttcgtatacg ggtccctacc ggtatatttt
     1501 cttctttttg gtcatgtttt tcataatacc cttttaaatc atccagaaaa tcctggtaat
     1561 ccttgctttt tacattggac ttcattggac cctcacatta atattaaagt gtttatgttg
     1621 tgtatagggg gtaataattg tgctgtcaag aatttaattg ctctgacacc aaacctgcat
     1681 acgagacttc atatttggcg taggagtatc ttactctgac accatacttg cataaggact
     1741 taattcattt aacacaataa ttattatgct ctgtcacctt tgggcgatag ttactgatga
     1801 tggctctgac accgtataca gtgcacatgc ttacacggag tttgcatatt gcagtattga
     1861 tatgtgctgc tatgaacgtc tctttccgtg tgacaaccat tattgctctg acaccatata
     1921 tcctttaaga tatgctctga cactgagttt taaat
//
LOCUS       DAOSZP010000450         1950 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_44010_length_1950_cov_3.226913, whole genome shotgun sequence.
ACCESSION   DAOSZP010000450 DAOSZP010000000
VERSION     DAOSZP010000450.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1950
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44010_length_1950_cov_3.226913"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..767)
                     /locus_tag="PK348_09165"
     CDS             complement(<1..767)
                     /locus_tag="PK348_09165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66424.1"
                     /translation="MKIRVVLTAILCICSVTMVFAASDGTALMLLGGSASMFGRADTG
                     VAYLGSDTFFINPAAIAKEERFIGSFNYGNLLFDYYNPSVVAGFPTSYGVIGGAFRLV
                     SGSDTLNGYQFVLGGGKEFTRRLSMGASLSFLLGNGPNSGAYFAGITLGSMYQTTINT
                     DIGKGFGLYYPTIGIAINAGLPMGSDRDFADMNQVTVGYTLPLYKRNDIQVSLLNDYT
                     FVQYDEIAVKVGTEGVYKNTYIVRLGFMAPGPYEYAD"
     gene            963..1436
                     /gene="bcp"
                     /locus_tag="PK348_09170"
     CDS             963..1436
                     /gene="bcp"
                     /locus_tag="PK348_09170"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015272987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HXK66425.1"
                     /translation="MNEDIILNKPAPQFKLPDKDKNMVDSNNFRGTWVVVYFYPKDNT
                     QGCTLEAIDFTKSIREFNNYNAVVVGISPDSPESHCRFYDKHNLNLILLSDTDHTVAK
                     EYGVWKKKSMYGKSFMGIERSTFLIDPEGRVRAVWRKVKVPGHVEEVLQTLKSFQ"
     gene            1448..>1950
                     /locus_tag="PK348_09175"
     CDS             1448..>1950
                     /locus_tag="PK348_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HXK66426.1"
                     /translation="MKLQEAIEARRAFRSLAPVTITETMVSELAKAASLAPSCYNNQP
                     WRFVFVYEPGKLDEVKKALSKGNEWALKASMIIAVFSKEKDDCVLKNRIYHHFDTGMA
                     TALLLLKATELGLVAHPIAGYDEEVVKKTCAIPDEFTVITLIIIGKKDSIIHDDLTEW
                     QKESEKNR"
BASE COUNT          643 a          351 c          414 g          542 t
ORIGIN      
        1 aaatcagcat attcgtacgg cccgggtgcc ataaaaccca accgcacaat atatgtattt
       61 ttataaacac cttcagtacc aacctttact gcgatttcat catattgcac aaaggtatag
      121 tcattgagca ggctaacctg tatgtcattt cttttataca gtgggagtgt atagccaacg
      181 gttacctgat tcatgtcggc aaaatccctg tcacttccca tgggtaaccc tgcgttaata
      241 gcaataccaa tggttgggta atataagcca aaacctttgc caatatcggt attgatggtg
      301 gtttgataca tgctgccaag agtgatacct gcaaaatatg cacctgagtt ggggccattc
      361 cccaaaagaa atgacagcga tgcacccatt gaaagccgcc gtgtaaattc tttaccacca
      421 cccagcacaa actgatagcc attgagggtg tcagagccag atacaagcct gaatgcgcca
      481 ccaatgacac catatgaggt tgggaatcct gcaactactg atgggttgta gtaatcaaaa
      541 aggagattgc catagttaaa tgacccaatg aaacgctctt cttttgcaat tgctgccggg
      601 tttataaaga aggtgtcaga gcctaagtag gcaacaccgg tatcagctct tccaaacatg
      661 ctggcgctgc ctccaagcaa catcagcgca gtgccatcag aagcagcaaa taccatggtg
      721 acagagcaaa tacatagtat agctgtcaaa acaacacgaa ttttcatgat tatcctcctg
      781 ttaagtgcgg atctttttat atttttttaa cattcattgc atccaaaata ttttttgtca
      841 agtggttata tttttaagtg gggcaattga caaagatcaa aataattagt attttagaat
      901 atactttatg attattgagc tgatttcaat ataaatgaat attttttaat gaggatagag
      961 tgatgaacga agatattata ttaaacaaac ctgcacccca attcaaactt cctgataaag
     1021 ataaaaatat ggtagatagc aataatttca gaggcacgtg ggtggttgtt tatttttatc
     1081 caaaagataa tactcaagga tgtaccctgg aagctatcga ttttaccaaa agtataaggg
     1141 agtttaacaa ttataatgca gttgttgtag gcattagccc ggatagtcct gaaagtcatt
     1201 gcagatttta tgataaacat aaccttaatc taattctctt atcagatact gatcatacag
     1261 tggcaaaaga atatggggta tggaaaaaaa agagtatgta tggcaaaagc tttatgggga
     1321 tagagcgaag tacattcctg attgatcctg aagggagggt cagagcagta tggcgaaagg
     1381 ttaaggtgcc aggacatgtt gaagaagtgt tgcaaacact taaatctttt cagtagagga
     1441 ggttactatg aaattacagg aagctattga agcacgaagg gcattcaggt cgcttgctcc
     1501 ggtgacaatt actgaaacaa tggtgtcaga gcttgcaaaa gcggcatccc ttgctccatc
     1561 atgctataat aatcaaccgt ggaggtttgt atttgtctat gagccaggca aactggatga
     1621 agtgaaaaaa gccctgtcaa aagggaatga atgggcactt aaggcttcca tgattattgc
     1681 ggttttcagc aaggaaaagg atgattgtgt attgaaaaat cgtatatatc accattttga
     1741 tactggaatg gctacagcac ttctgttact taaagcaact gaattagggt tggtagctca
     1801 ccccattgca gggtacgatg aagaagtagt taaaaaaaca tgtgctatac ctgatgagtt
     1861 tactgtaatt accttaatta tcataggtaa aaaagatagt ataatacatg atgatttgac
     1921 cgaatggcag aaggaaagcg aaaagaaccg
//
LOCUS       DAOSZP010000451         1949 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_44037_length_1949_cov_3.204857, whole genome shotgun sequence.
ACCESSION   DAOSZP010000451 DAOSZP010000000
VERSION     DAOSZP010000451.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1949
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44037_length_1949_cov_3.204857"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..531
                     /locus_tag="PK348_09180"
     CDS             <1..531
                     /locus_tag="PK348_09180"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HXK66427.1"
                     /translation="IEDKRAIEHLIQGLSDPNPQFVEACARALGAFSNRLIIKPLTDL
                     ILNENYPIIKSSAIYALSKSIDNNTAHSLLTSFHDAPLIVKKRLIQLASYFPSEESET
                     LLQQALTIDDEDCKMLAIQGLKHIGTEKSIDCVRSAIINENPFISDEARTALKEIIER
                     KQRGNIKTLDNYDDFI"
     gene            791..988
                     /locus_tag="PK348_09185"
     CDS             791..988
                     /locus_tag="PK348_09185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66428.1"
                     /translation="MNIKEVILYDADSLEYSGKILVEGTSWEFCDVSNDFLVKFTKGM
                     PLKAVLQCLISFNIVYDIIEM"
     gene            998..1873
                     /locus_tag="PK348_09190"
     CDS             998..1873
                     /locus_tag="PK348_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013885521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - cellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HXK66429.1"
                     /translation="MQKHLTPETILMIIKFQKSELTEHHIYNLLASHIKNHHNARILK
                     KVAEDEKKHYEFWKSYTHSDVKPDRWKIIKYYIISRLFGLTFGIKLMERGEEQAQASY
                     KSLEPIIPEVKKIIKDEHAHEKELYILLEEEHLQYVGSVVLGLNDALVELTGTLAGLS
                     FALANTHLIALAGLITGIAAALSMAASEYLSSKAEDAKDALKSAIYTGIAYIITVVLL
                     IIPYFVAGHYLVALGITLVIALLIIFFFNYYIAIAKDLNFKKRFSEMAIISLGVACIS
                     FAIGFFIKKYMGIDV"
BASE COUNT          641 a          353 c          372 g          583 t
ORIGIN      
        1 attgaggata aacgtgcaat tgaacatctt attcagggtc tttctgatcc taacccccaa
       61 ttcgtggaag catgtgcacg ggcactgggt gcattcagca acagattaat tatcaaacca
      121 ttgaccgatt taatactgaa tgagaattac cccattatta aatcaagtgc aatttatgcc
      181 ctttcaaaaa gtattgataa caatacagca cacagtttgc ttacctcttt tcatgatgcc
      241 ccacttattg taaaaaaacg ccttatacag ctggcttcat atttcccatc agaagaatca
      301 gaaacgctgt tgcaacaggc tctgactata gatgatgagg attgcaagat gctggctata
      361 cagggattaa aacatattgg caccgaaaaa tccattgact gtgtacgatc agcaattata
      421 aatgaaaacc catttataag cgatgaagcc aggacagcac taaaagaaat aattgaaaga
      481 aaacaacgcg gaaacataaa aacacttgat aattatgatg attttatttg aatagtccag
      541 aagtagtctg tcacataaca ttctgaatat ttatctggta attctgactc caataatgct
      601 gggagaagaa tctcgaagta taaaacttgg attcttcgtc ccaatgcaat cgggacgaag
      661 aatgacatac acacttcgtc atcctgaact tgattcagga tctttggatg atggtggggt
      721 gcaggagatt ctgagtttag ttcagaatga tagtgtataa ttttactttc aggagtgttt
      781 gagtatgagt atgaacatca aagaagtaat tttgtatgat gcagattcac tggaatactc
      841 aggcaagatc ctggtagaag gcaccagctg ggagttttgt gatgtttcaa atgactttct
      901 ggtaaaattt acaaaaggaa tgccgcttaa ggctgtcctc caatgtctta tctcatttaa
      961 tatagtgtat gatatcattg agatgtaggg aaaacttatg caaaagcatt tgactcctga
     1021 aaccatatta atgatcatta aatttcagaa aagtgaatta accgaacacc atatctataa
     1081 ccttttagca tcacatatta agaatcatca taatgcacgt atattgaaaa aagtagcaga
     1141 agatgaaaaa aagcattatg agttctggaa aagctacacc cattccgatg ttaaacctga
     1201 tcggtggaag ataataaaat attatattat ctcccgttta tttggtctga catttggcat
     1261 aaaattaatg gaacgcggcg aagaacaggc tcaagcttct tataaatcat tagaacccat
     1321 tatccccgaa gtaaaaaaga tcatcaaaga cgaacatgcc cacgaaaaag aattatatat
     1381 cttgcttgaa gaagaacatc ttcagtatgt tggttcagta gtactggggc ttaacgatgc
     1441 tctggtagaa ttaactggca cattagctgg tctttcattt gccctggcaa atacacatct
     1501 tattgcctta gcaggtctta taaccggaat tgcagcagcg ttatccatgg cagcatcaga
     1561 atacctttca agcaaagctg aagatgcaaa agatgcgtta aagtcagcca tctacacagg
     1621 aattgcctat atcatcacgg tggtgctttt aattattccc tactttgtgg caggccatta
     1681 cctggttgct cttggcatta cgctggtaat tgcgctgctg ataatctttt tctttaatta
     1741 ctacattgcc attgcaaaag accttaattt caaaaaacgt ttttctgaaa tggcgataat
     1801 aagtcttggc gtagcatgta tatcgtttgc tattggcttt tttattaaaa aatatatggg
     1861 cattgatgtg taatgttacg gcgataggcg cctgcaattc cactgttcat tggaaaaata
     1921 gctgcgttct aattcaattg ccatatcgt
//
LOCUS       DAOSZP010000452         1948 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_44076_length_1948_cov_3.452192, whole genome shotgun sequence.
ACCESSION   DAOSZP010000452 DAOSZP010000000
VERSION     DAOSZP010000452.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1948
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44076_length_1948_cov_3.452192"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="PK348_09195"
     CDS             complement(<1..293)
                     /locus_tag="PK348_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXK66430.1"
                     /translation="MGEETLKSRIISDNPFRALRVYNFRLFFMGQFISLVGTWIQQLA
                     SSWLMYRLTASSMWLGLLAFSAQLPIFLFTPITGVVADRYNRHHILMITQILL"
     gene            complement(275..709)
                     /locus_tag="PK348_09200"
     CDS             complement(275..709)
                     /locus_tag="PK348_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004578305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine dimer DNA glycosylase/endonuclease V"
                     /protein_id="HXK66431.1"
                     /translation="MRLWSIHPHYLDPQGIVAVWREGLLAKKVLEGNTKGYRHHPQLI
                     RFANYIKPLEAIQAYLYWIYEEAKRRGYQFSADKIEAKPLVALIPATYGQLQYEFEHL
                     LKKVYIRNRVWYQELSHKKKDIQCNPVFYCIEGDSEPWEKKH"
     gene            894..1895
                     /locus_tag="PK348_09205"
     CDS             894..1895
                     /locus_tag="PK348_09205"
                     /inference="COORDINATES: protein motif:HMM:NF024598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HXK66432.1"
                     /translation="MKKIAVLLVCIIAIVAILIGFEMFGKSDTQVTVEEVKQQLPAPE
                     FYKGIYLNSQSGRDINLLKNFISKAKDAGFNAVVIDVHNESNNKFLIPKENIDYVISQ
                     GIYPIARVVMFPDGLKVYPIPDELIKGRLELAKKACEIGFREIQFDYIRFNDYGILKH
                     VPLEQRYKTIEGFLARAKTELQPYNVKVACDVFGRIPLNTNDTIGQRMEGLDQVVDVI
                     CPMAYPSHYTWSKKLMADPYYTVFITGKRAKERTKNAQIVMYIQAFKIRVAMSGLSFQ
                     EYMAKQIQAVHDAGVKGFIWWNAAQQYDEPFAVTRQFYTNFKEQSISKADNSKKEAI"
BASE COUNT          630 a          384 c          372 g          562 t
ORIGIN      
        1 cataaaagga tctgggtaat catcaatata tgatgcctgt tatagcggtc agccaccaca
       61 ccggtaatgg gcgtgaacaa aaaaatggga agctgtgcag aaaatgccag taaccccaac
      121 cacatggatg atgcagttaa acggtacatc agccagcttg aggccagttg ctgaatccat
      181 gtcccaacca gtgaaataaa ctgacccata aaaaagagcc tgaaattata taccctcagt
      241 gccctgaatg ggttatcaga tataattctt gattttaatg tttcttctcc catggttcac
      301 tatcaccttc aatacagtag aacaccggat tacattgtat atcctttttt ttgtgcgata
      361 gttcctgata ccagactctg ttgcgtatat acactttttt tagcagatgc tcaaattcat
      421 attgtagttg cccatacgtg gcagggatta atgcaaccag tggttttgcc tcaatcttat
      481 cagcagaaaa ctgatagccc cgcctttttg cttcttcata gatccagtaa agatatgcct
      541 gtatagcttc aagcggctta atgtaattgg caaaccgtat caattgcgga tgatgcctgt
      601 accctttagt attcccttca agtaccttct ttgcaagcaa cccttcccgc catacggcaa
      661 caattccctg agggtctaaa tagtgtggat gtattgacca taaccgcata agatattgta
      721 tataactatc gatagaatat actactacac attgtattac ataacaaccc taaattataa
      781 gtaaataaaa attttagcac tgttttttgg tacaacgaat atatatttta gtcgacaaat
      841 taagcaatag tatcaccata taccacaatc ataacttaca gaaaggaacc actatgaaaa
      901 agattgccgt actgcttgta tgcattatcg ctatcgtagc tattcttatt ggctttgaga
      961 tgtttggcaa aagcgatacg caggtcactg ttgaagaagt caaacagcaa ctacctgcac
     1021 ctgagtttta taaaggtatt tacttaaatt cacaatcggg gcgcgatatc aatcttttaa
     1081 aaaattttat ttcaaaagca aaagatgcag gattcaacgc agtagtcatt gatgtacaca
     1141 atgaatccaa taataagttt ttaatcccca aagaaaatat tgactatgta atttcgcaag
     1201 gcatataccc cattgctcgc gtggttatgt tccccgatgg gctaaaagtg tatcccatcc
     1261 ctgatgagct tattaaaggg aggctggagc ttgcaaaaaa agcctgtgaa attggcttca
     1321 gagaaataca gtttgattat atacggttta atgactatgg tatactgaaa catgtacccc
     1381 ttgaacagcg ctataaaact attgagggat tcttagcccg agcaaaaact gaattacagc
     1441 cgtataatgt caaggttgcc tgcgatgtat ttggccgaat tccacttaat accaacgata
     1501 ccattggtca gcgtatggaa gggcttgatc aggttgttga tgtaatctgc cccatggctt
     1561 atccttccca ttatacatgg agtaaaaaat taatggctga tccatattac acagtattta
     1621 tcaccgggaa aagagcaaaa gaacgtacaa agaatgctca gattgttatg tacatccagg
     1681 cgtttaaaat acgggtagca atgtcaggat tatcgtttca ggaatacatg gcaaaacaaa
     1741 tacaggccgt tcatgatgct ggtgtaaaag gctttatatg gtggaatgct gcacagcagt
     1801 atgatgaacc ttttgcggtt accaggcagt tttacaccaa cttcaaagaa cagagtatat
     1861 caaaagcgga taacagcaaa aaggaagcaa tttaaaacta acaaaaatat tcttgactat
     1921 tggctgccgg tggatattta ttttggcg
//
LOCUS       DAOSZP010000453         1948 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_44079_length_1948_cov_3.229266, whole genome shotgun sequence.
ACCESSION   DAOSZP010000453 DAOSZP010000000
VERSION     DAOSZP010000453.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1948
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44079_length_1948_cov_3.229266"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..359)
                     /locus_tag="PK348_09210"
     CDS             complement(<1..359)
                     /locus_tag="PK348_09210"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HXK66433.1"
                     /translation="MCGQQFTYNLYELFQEQAHKFPKKIMLHYNNVPVLYKDALHKVH
                     KIAHALRKRGVHSKTTVIIQMGNTPYFVYSFFAILQCNAIPVLVNPLARQFELHHYIT
                     VTRPDCIITTSFMAERL"
     gene            552..995
                     /locus_tag="PK348_09215"
     CDS             552..995
                     /locus_tag="PK348_09215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66434.1"
                     /translation="MIVLTFIIILQISLAVHVYYIISYISKKQDKDFKGFLFTSMINI
                     FLGIFLSVFILLFPQDLKAINLERILFIESGVIFFGMLYVKTKIAVNIYKRTKDPQYF
                     HYSYFGKKVLHPNVVKMPELFTYFLTLPLTLVCGAYFIVKLGCGK"
     gene            complement(1000..>1948)
                     /locus_tag="PK348_09220"
     CDS             complement(1000..>1948)
                     /locus_tag="PK348_09220"
                     /inference="COORDINATES: protein motif:HMM:TIGR01883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HXK66435.1"
                     /translation="NVLIRIPGDKSYNPVLFSCHMDTVTPCENIQPIIKDNKIVSDGR
                     TILGADDKSAIAAFLEAISLLQQHSLPHGHIEFLFSCAEEVGLFGAKGFDLSVVKAKY
                     AFVFDSDGPVGKIIVAAPYHISMKIIIKGKAAHAGMEPEKGISAIRVLSEIIAAIPHG
                     RLDSETTVNVGTVKGGRATNIVAEEAECTLETRSRQKTRVLSVKREIERTAKTIAQKY
                     KAKVKIESTMDYEGYTIRDNDPIVLIASNALRSIGIKPALAVSGGGSDTNIFNAHGIK
                     AINLSSGMRQVHTTKEYIIIQDLVNVASLVVSIIQNVKH"
BASE COUNT          591 a          386 c          309 g          662 t
ORIGIN      
        1 aatagtctct cagccataaa gcttgtggta atgatacaat caggacgcgt aacagtaata
       61 tagtgatgca attcaaactg ccgggctaaa ggattaacca gaacaggaat ggcattgcac
      121 tgcaaaatgg caaaaaagct ataaacaaaa tatggtgtat tacccatttg tataatcacc
      181 gtagttttgc tatgaactcc acgctttcgc aatgcatgtg caattttatg tactttatgt
      241 aaggcatcct tatacagtac aggcacatta ttatagtgta gcattatttt ctttggaaat
      301 ttgtgtgcct gttcctggaa taattcatac agattatacg taaactgttg tccacacata
      361 gttcattatc cctttaagcg atagtatata gtatcatgat ttatactaaa aagacaagta
      421 aatgtttaca ggtgttccaa tgattttctt gacttatgct taccagtttg tataatacaa
      481 aagattataa tgtataaccc ccgccaaagg tagcggggtt ataattattc aataattaca
      541 tacgagcata tatgattgtt ctaacattta ttattatcct gcaaatatca ctggccgtcc
      601 acgtctatta tattataagc tatatttcaa aaaaacagga caaagacttc aagggatttc
      661 tttttacttc aatgattaat atttttttag gaatattttt gtcagttttt attttattat
      721 ttccacaaga tcttaaggca atcaatttag aacgcatact ctttattgaa tctggagtaa
      781 ttttttttgg catgctatat gtaaaaacaa aaattgcagt aaatatatac aaacgcacaa
      841 aagatcccca gtattttcat tattcatatt ttggcaaaaa ggtactgcat cccaatgtgg
      901 taaaaatgcc cgaacttttt acctactttt taacacttcc ccttacactg gtatgcggtg
      961 catattttat agtaaaatta ggatgtggga aataatatat cagtgtttta cattctgaat
     1021 aattgacacc accaacgatg caacattaac aagatcctgt attattatat attcttttgt
     1081 ggtatgcact tgccgcatgc ccgatgaaag gtttattgct ttaattccat gcgcattgaa
     1141 tatattggta tcactgcctc cacccgaaac tgccagagcc ggctttatgc caatacttcg
     1201 taacgcatta gatgcaatta aaacaatggg atcattgtcc ctgatggtat agccttcata
     1261 atccatagtg ctttcaatct ttacttttgc tttatatttc tgtgcaattg tttttgctgt
     1321 tctttcaatt tctcttttta ccgaaagcac tctggtcttt tgccgtgaac gggtttcaag
     1381 agtacattcc gcttcctccg caacaatatt ggttgcccgt ccacctttta ctgttccaac
     1441 atttactgtt gtttcgctgt caagcctgcc atgggggata gcagcaataa tttcagaaag
     1501 aactcgtata gcacttatcc ctttttcagg ttccataccg gcatgtgcag ccttcccctt
     1561 tataataatt ttcatgctga tatgataagg tgctgccaca ataattttac ctactggtcc
     1621 atcgctatca aacacaaatg catactttgc tttaaccact gacaggtcaa acccctttgc
     1681 cccaaacaat ccaacttcct ctgcacatga aaataaaaac tcaatatggc catgtggtaa
     1741 actatgctgt tgtaacagtg atattgcttc tagaaacgct gcaattgccg atttatcatc
     1801 agcccctaat attgtccttc catccgatac tatcttatta tcttttatga ttggttgaat
     1861 attttcacac ggtgtgaccg tatccatatg acatgaaaaa agaacaggat tgtagctttt
     1921 atcaccagga atacggatca acacattg
//
LOCUS       DAOSZP010000454         1947 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_44109_length_1947_cov_2.447146, whole genome shotgun sequence.
ACCESSION   DAOSZP010000454 DAOSZP010000000
VERSION     DAOSZP010000454.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1947
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44109_length_1947_cov_2.447146"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1947)
                     /locus_tag="PK348_09225"
     CDS             complement(<1..>1947)
                     /locus_tag="PK348_09225"
                     /inference="COORDINATES: protein motif:HMM:NF014602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HXK66436.1"
                     /translation="AFSIEGIHPSAFPFIVATSFNTLQTQTIVVTTDYHTMNRFVADI
                     SAFVNEDTIFPFPPLEVLPYEYVNPSSSIERDRITALYQLLSGKPVIVVATIESLLRA
                     IPHKEFFLKKGITIERNQEYPFEVIIETLINYGYERETRVDRYGQFSVKGGIIDIYLP
                     NAANPVRLDFFGDTCESIRYFDPMTQVSLGEVETITIYPRREILLSHSEKEKIQKELL
                     RGDSNRNQQAVESLQKANVPGIYDLFPLAIQTDTITSFAHNPHIFLCDPPQLKAKLSE
                     IERTYHQLYNHKKDSTFCIPASQLLLSDSYQHIESQAVKLHPFITDSKNVWHLKGLPS
                     FQGRIQDVRNECAKRIEEGWHIVVSTSFEGQARRLYDLFNEFKPQEHFDTYNPKKHFG
                     IIISNLHEGLEIGSIKTCIITDHEIFGKSYRKKKHFKQKYSKPIETFLELSPGDYVVH
                     INHGIGIFKAIERMSAGGVERDFLVIEYADGDKLYVSLDQINMVQRYVGLDGRQPRID
                     ALGKKSAWNRIKERVQQSVEEIAQDLLKIYSQRKALKGFQYPADTMWQEEFESKFEYE
                     ETPDQITAIEDVKDDMEKSQPMDRLVCGDVGFGKTEVAIRAAFKAVMAGKQVAILVPT
                     TVLAMQHYTTFCKRFADYPVTIEML"
BASE COUNT          514 a          388 c          426 g          619 t
ORIGIN      
        1 agcatctcga tagttaccgg gtaatcagca aaacgcttgc aaaaggttgt atagtgctgc
       61 attgccagta ctgttgtggg aacaagtatt gcaacctgtt ttcctgccat taccgctttg
      121 aatgcagccc ggatagccac ctcggttttg ccaaatccaa catcgccaca caccaggcgg
      181 tccattggct gagatttttc catgtcatct tttacatctt caatggctgt gatctggtca
      241 ggggtttctt catattcaaa ctttgattca aattcttcct gccacatggt gtctgcggga
      301 tactgaaatc cctttaatgc ttttcgctgt gagtaaattt ttaataaatc ctgtgctatt
      361 tcttccactg attgctgaac acgctcttta atgcggttcc atgcagattt tttaccaagt
      421 gcatcaatac gtggttgacg gccatcaagc cccacatatc gctgtaccat atttatctgg
      481 tcaagtgaaa cgtacagctt atcaccatca gcatactcta tgactaaaaa gtcacgctca
      541 acgccaccag cactcatgcg ttcaatggct ttgaaaatcc ctatgccatg gttaatatgc
      601 acaacatagt cgccaggcga tagttctaag aaagtttcta ttggctttga atatttctgt
      661 ttgaaatgct ttttctttcg gtacgatttg ccaaaaattt cgtggtcggt gatgatacat
      721 gtcttgatgc ttcctatctc aaggccttca tgaagatttg aaataataat gccaaaatgc
      781 tttttggggt tatatgtatc aaaatgctcc tgtggtttaa attcattgaa taaatcgtac
      841 agcctgcgtg cctgcccttc aaacgatgtg ctaacaacaa tatgccatcc ttcttcaatg
      901 cgttttgcgc actcattgcg tacatcctga atccttccct ggaatgatgg taatcctttt
      961 aagtgccata cgttcttgga atcggttatg aatgggtgca gtttgacagc ctgcgattca
     1021 atgtgctggt aactatcgct tagcaataat tgcgatgctg gaatgcagaa ggtgctgtct
     1081 tttttgtgat tgtacaattg atggtaggtt cgctcaattt cagacaattt tgctttcagc
     1141 tgtggtggat cgcacagaaa tatgtgtgga ttgtgcgcaa atgaggttat tgtatcggtt
     1201 tgaattgcca gtgggaacag atcgtatatg ccaggtacat ttgctttttg caatgactcc
     1261 actgcctgct gattacggtt actatcgcca cgcagcaatt ctttttgtat tttttctttt
     1321 tcagaatgag acaacaaaat ttcacggcgt ggataaatgg ttatggtttc tacctcacca
     1381 agtgatacct gcgtcatggg gtcaaagtag cgaatggact cacaggtgtc gccaaaaaaa
     1441 tctaatcgga caggattggc tgcattggga agatagatat cgatgatacc acctttaaca
     1501 gaaaattgtc cgtatcgatc aacgcgtgtt tcgcgttcat agccatagtt gatgagtgtt
     1561 tcaatgatta cttcaaatgg atattcttga tttcgttcaa tggtgatgcc tttttttaaa
     1621 aaaaattctt tatgagggat agctcgtagc agtgattcaa tggttgcaac cacaatgaca
     1681 ggttttcctg aaagaagctg gtacagcgcg gtgatacgat cacgttcaat agatgatgat
     1741 gggttaacat actcatacgg aagtacttct aagggtggaa aagggaaaat ggtgtcttca
     1801 ttgacaaatg ctgatatgtc ggcaacaaag cggttcattg tgtgatagtc ggtggtaacc
     1861 acaatggttt gcgtttgcag ggtgttaaat gatgttgcca caataaatgg aaacgcagat
     1921 gggtgaatgc cttctatgga aaaggct
//
LOCUS       DAOSZP010000455         1932 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_44552_length_1932_cov_2.956846, whole genome shotgun sequence.
ACCESSION   DAOSZP010000455 DAOSZP010000000
VERSION     DAOSZP010000455.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1932)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1932)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1932
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44552_length_1932_cov_2.956846"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..447)
                     /locus_tag="PK348_09230"
     CDS             complement(<1..447)
                     /locus_tag="PK348_09230"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HXK66437.1"
                     /translation="MIKGTVLDFAIIVCYFLFILGFGTFFGKYVKSTRDFFFGGQRFS
                     WWLIGFSCVATTVGSYSFIKYSAVAYQYGFSSTMTYLNDWFILPLFVFIWLPIIYYSR
                     ITSIPEYFEKRFNSTTRTISVFILLVYLLGYIGINLYTIGVAYNAMF"
     gene            complement(451..651)
                     /locus_tag="PK348_09235"
     CDS             complement(451..651)
                     /locus_tag="PK348_09235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66438.1"
                     /translation="MNSPWLPFIYQYSIFTIFFIFTTMIAIKKDVISLSHPTERRILF
                     EIITGYLFFIVFHASMIIFAGV"
     gene            complement(648..1262)
                     /locus_tag="PK348_09240"
     CDS             complement(648..1262)
                     /locus_tag="PK348_09240"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HXK66439.1"
                     /translation="MKSIVLHNTYLCMRHAQSTANEKHIIISDPATGISNYGLTKEGE
                     RQVLEAIVTNKEMRNISYIYTSDFLRAYQTATIVSRLCNNVPIITDARLRERYFGLFE
                     GTNSENYHKVWDMDCTSEFDVACYNVEPIHTVARRMLSFLLDSEIHPTNQKILVVSHG
                     DPLQILYAVTRGLAAHQFKSLPHFANAEIRILPSHFTIPEEYLS"
     gene            complement(1306..>1932)
                     /locus_tag="PK348_09245"
     CDS             complement(1306..>1932)
                     /locus_tag="PK348_09245"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXK66440.1"
                     /translation="HTHWDHIQGFPFFDLAYNKHASIYFYGGHSVSTLEKLILGQMDR
                     EYFPVTLFELSSNIHFVELSHNPFTINDITIWYTHLFHPGLSLGFRVEYNNHVFVYAS
                     DNEVLNDPELGDYNLKNIGNLIQDADILVADCQYTPQEYATRIGWGHSSIESVAGVAS
                     RFNVKHLITFHHDPNHTDRDITRAIKKARSLSFPIRITAAKEGDILYI"
BASE COUNT          648 a          346 c          373 g          565 t
ORIGIN      
        1 aaacatggca ttatatgcaa cgccaattgt ataaagatta atacctatat agcctaataa
       61 atacactaaa agtataaata cactgattgt tcgtgtggta ctgttaaacc gcttttcaaa
      121 gtattcaggt attgatgtta tccgtgaata atatattata ggaagccata tgaatacaaa
      181 tagtggcagg atgaaccaat cattgaggta tgtcattgta gaagaaaatc cgtactgata
      241 tgctacagct gaatatttaa taaaactgta tgaccctacc gttgttgcca cgcagctaaa
      301 tccaataagc caccagctaa aacgttgacc gccaaaaaag aaatctcttg tactttttac
      361 atatttgcca aagaatgtcc caaatcccag tataaacaaa aaataacata caataatggc
      421 aaaatcaagt actgtaccct taatcatgtc ttacactccg gcaaagataa tcattgatgc
      481 atgaaatacg ataaaaaaca gatagcctgt gataatttca aagagtatac ggcgctcagt
      541 aggatgagaa agtgatataa catccttctt tatagctatc attgtggtga aaatgaagaa
      601 tatggtgaaa atactgtact gatatatgaa aggcagccag ggtgaattca tgatagatat
      661 tcctccggaa ttgtaaagtg tgatggcaat atgcgtattt cagcatttgc aaaatgtggt
      721 aaagatttaa actgatgtgc agcaagacca cgggtaacag catacagaat ttgcaagggg
      781 tcaccatgtg ataccacaag aatcttttga ttggtagggt gtatttcgga atcaagtaaa
      841 aatgatagca tacgtcgtgc aacggtatga ataggttcaa cattatagca ggcaacatca
      901 aattctgatg tgcaatccat atcccatact ttgtggtaat tttcgctgtt ggttccctca
      961 aacaggccaa aataacgctc acgcagtcgt gcatcagtta tgataggaac attgttacaa
     1021 agccgtgaaa caattgtagc agtttgatat gcgcgcaaaa aatccgatgt atagatgtat
     1081 gatatattgc gcatttcttt attagtaact atcgcctcta acacctgacg ttccccttcc
     1141 tttgtcagtc catagttgct tataccagtt gcagggtcac tgatgataat atgcttttca
     1201 ttagcagtgc tctgggcatg gcgcatgcaa aggtatgtat tatgtaacac aattgatttc
     1261 atacatgtgc cactatagat aaagaaaata taaaatcaat attttttaaa tataaagaat
     1321 atccccttct tttgcagcag ttattctgat ggggaaagac aatgatcgtg ctttctttat
     1381 tgctcgggta atatcacgat cagtatgatt gggatcatgg tggaatgtga ttaaatgctt
     1441 aacattaaaa cgtgaagcaa cgccggcaac ggattcaata gaagaatgcc cccagcctat
     1501 gcgtgttgca tactcctggg gtgtatactg gcagtcagca acaagtatat cggcatcctg
     1561 tattaaatta cctatatttt taaggttata atcccctaac tccggatcat ttaatacttc
     1621 attatcactg gcatacacaa agacatggtt attgtattca acacggaaac ccaatgataa
     1681 tcccggatgg aaaagatggg tgtaccatat tgtaatatcg tttattgtga atgggttgtg
     1741 cgatagctca acgaaatgga tgttagatga cagctcaaat aaagttacgg ggaaatactc
     1801 tctatccatt tgccctaaaa taagcttttc cagtgttgag acgctgtgcc ctccataaaa
     1861 gtaaatagaa gcatgtttat tatatgccag gtcaaaaaac ggaaatccct gtatatggtc
     1921 ccagtgagta tg
//
LOCUS       DAOSZP010000456         1926 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_44737_length_1926_cov_3.956708, whole genome shotgun sequence.
ACCESSION   DAOSZP010000456 DAOSZP010000000
VERSION     DAOSZP010000456.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1926)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1926)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1926
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44737_length_1926_cov_3.956708"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /locus_tag="PK348_09250"
     CDS             complement(<1..246)
                     /locus_tag="PK348_09250"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HXK66441.1"
                     /translation="MIYIAFDIDGTLYDCSPVVGMAFEQGIDTFLVQHPELNLRKPST
                     DEIMKLVGIPVDEIFALLFPALSQELSHTLNDYCTAKL"
     gene            374..1294
                     /locus_tag="PK348_09255"
     CDS             374..1294
                     /locus_tag="PK348_09255"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HXK66442.1"
                     /translation="MNLSVTIGNLQLKNPITVASGTAGYGEELSQFVDIGSLGAIFTK
                     GLSLKPRAGNPGPRIAETPSGMLNSIGLENVGLEAFLSHKLPYLQKANATVIPNIAGH
                     SIEENVELAKTLSHTNGIAAIELNVSCPNVKEGGIAFGQDVAIFSRLLEKVRSVTRGV
                     LIVKLSPNVTDITQFAKAAKEIGADAVSAVNTFVGMKIDIATGKPYFANTFAGLSGPA
                     IKPIALRMVYQIVQNVDIPVIGIGGIFSFEDVLEFLMAGAQAIQIGTGNLIHPDISAV
                     ILQQLENYMKMHSIDTVKDLIGIAQKRSNQ"
     gene            1291..>1926
                     /locus_tag="PK348_09260"
     CDS             1291..>1926
                     /locus_tag="PK348_09260"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HXK66443.1"
                     /translation="MKYWNKRLQKMTEYIPGEQPDNLNEFIKLNTNENPFPPSSAVLD
                     ALQKACNGDLRRYPNPNAQMVRELFAKKNSLAADNVFVANGSDEIFTLLFRGFIEPDG
                     IAAFPYPSYALYYTMAEANGIAYDKITLDDNFDIPFDEFLKKKYNLVIIANPNNPTGK
                     GVPIDQIKKFCSKFKGLLVVDEAYVDFYNETAMPLVKEFDNIVITRSFSKSY"
BASE COUNT          614 a          359 c          381 g          572 t
ORIGIN      
        1 aagttttgcg gtacaataat cattaagcgt gtgtgatagt tcctgtgaaa gagctgggaa
       61 gagtaaagca aatatctcat caacaggaat cccaacaagc ttcatgattt catcagtgga
      121 tggtttgcgc aaattaagct cgggatgttg cactaaaaat gtatcaatac cctgttcaaa
      181 tgccatccca acaacaggtg agcaatcgta taatgttcca tcgatatcaa atgcaatgta
      241 tatcatggtg gaaatatttt ttaattacag gttttggcaa caaaattttg tttcaataat
      301 ttattttctt gactcatttg tggtataatt atacttcaca attgcaaatt ataactatgc
      361 tgctggaaca accgtgaatc tttctgtaac aattggcaat ctacaactaa aaaaccccat
      421 aaccgttgca tcaggcactg caggttatgg cgaagaactt tcgcaatttg ttgatattgg
      481 tagtttaggt gcaatcttta caaaagggtt atccttaaaa ccgcgtgcag gtaatcctgg
      541 tccacgtata gctgaaacac cttcagggat gcttaattcc ataggtcttg aaaatgttgg
      601 acttgaagca ttcttatcgc acaagctgcc gtatttacaa aaagccaatg ctactgtaat
      661 acctaacatt gcagggcact caattgaaga aaatgttgaa ctggcaaaaa cactttcaca
      721 caccaatggc atagcagcta ttgaactcaa tgtgtcatgc cctaatgtaa aagaaggggg
      781 cattgccttt ggacaggacg ttgccatatt ttcaagatta ctggaaaaag ttcgcagtgt
      841 aacccgtggt gttttaattg ttaagctttc accaaatgtt actgatatta cacaatttgc
      901 taaagccgct aaagaaattg gcgctgatgc agtatcagca gtaaatacat ttgtaggaat
      961 gaagattgat atagcaacag gaaagccata ctttgcaaat acatttgccg gtttaagtgg
     1021 ccctgcaatt aaaccaatag cattacgcat ggtatatcag attgtccaga acgttgatat
     1081 accggttatt ggcattggcg gcatctttag ttttgaagat gtgctggaat ttttaatggc
     1141 tggtgcgcag gccatacaga taggtactgg aaaccttatt catcctgata tatccgctgt
     1201 tattttgcaa cagcttgaaa attacatgaa aatgcattca attgatactg taaaagactt
     1261 gattggcatt gcacaaaaaa ggagtaacca atgaagtact ggaacaaacg tctgcagaag
     1321 atgaccgaat acatccccgg tgagcagccc gataatctga atgaattcat taaactcaac
     1381 actaatgaaa atccgtttcc gccatcatcg gctgtactgg atgcattgca gaaagcatgc
     1441 aatggggatc tgcgccgcta tcccaatccc aatgctcaga tggtacggga actgtttgca
     1501 aaaaagaatt cattagcagc tgataatgtt tttgttgcca atggttctga tgaaatattt
     1561 acattgctgt tcagaggatt tattgaacct gatggcattg cggcgttccc ctatccttcg
     1621 tatgcactct attataccat ggctgaagca aacgggattg cgtatgataa aattaccctt
     1681 gatgataact ttgatatacc atttgatgaa ttcttaaaaa agaaatataa tctggttatt
     1741 atagccaatc ccaataaccc taccggtaaa ggggttccca ttgaccagat aaaaaagttt
     1801 tgcagcaaat tcaaagggtt gcttgttgtt gatgaggcgt atgttgactt ttacaatgaa
     1861 acagctatgc cactagtcaa agaatttgat aatattgtga taacccgttc attttcaaaa
     1921 tcatat
//
LOCUS       DAOSZP010000457         1922 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_44856_length_1922_cov_2.600964, whole genome shotgun sequence.
ACCESSION   DAOSZP010000457 DAOSZP010000000
VERSION     DAOSZP010000457.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1922)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1922)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1922
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44856_length_1922_cov_2.600964"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            99..1010
                     /locus_tag="PK348_09265"
     CDS             99..1010
                     /locus_tag="PK348_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012066347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HXK66444.1"
                     /translation="MRKLTHIIEKYFLVWVVLLSLCGYHFPSAFKPLVNYIPLFLGII
                     MFGMGITLKPSDFIVVVKFPLALVTGVVAQFIIMSLVALCLCMLFNLPPLLAIGVILV
                     GTCPGGTASNVITYLSKGDVALSVAMTTVSTLLSPALTPLLTYLLAGRWVPVPIVSMV
                     ISIVQVIIVPVALGIMVRLLLKKYIDRVLMVLPSVSVVAIVTIIAGITAANAHALRTV
                     GFITVLVVILHNVTGLALGYAAASLLGFDKSVRKTIAIEVGMQNSGLAVSLAIMHFEA
                     LAALPGAIFSIWHNISGSIAAWWWRRR"
     gene            complement(978..>1922)
                     /locus_tag="PK348_09270"
     CDS             complement(978..>1922)
                     /locus_tag="PK348_09270"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HXK66445.1"
                     /translation="KTVVEHVRKASTIINNFSRDITAIIHEHSAIFEKSRAISTDIIE
                     SFTVLKEATLKEIENNKEINDITKGLGTDLTNAAEAGKQASGEAKKIQELSDDCNRKL
                     QDARQTIERLRDESKMIEEISQTINEISDQTNLLSLNAAIESARAGEHGRGFAVVADE
                     ISKLADKSQSSAKEISGIIGRSVQRILSAAVQIDDASLALIEIIQFLEANRSFFLKFQ
                     ELVSSQDKNINTVIGHLGNSLKYTQYINELTEKNATAVEQLQQALEQIHTFYDNLTDI
                     SMTFKKLAGELTIHVDNLQKTLGDNSINLPSSPPPCSN"
BASE COUNT          448 a          384 c          410 g          680 t
ORIGIN      
        1 gcgagtatta cgatataatg tttgcaaaat ttactcaacg acctcttttg ggacaccccc
       61 ggtaactatc gccacccttt ttactatggt acacctgtat gcgtaaactt actcacatca
      121 tcgaaaaata ttttttagta tgggttgttc ttttatcttt atgcgggtat catttcccat
      181 ctgcctttaa gcctctggta aactacatac cgcttttttt aggaatcatc atgtttggga
      241 tgggcataac ccttaagcct tccgacttta ttgtggtagt aaaattcccg ctggcgcttg
      301 tcaccggtgt agttgcacag tttataatca tgtcgcttgt tgctttgtgt ttgtgcatgc
      361 tttttaacct gccgccacta cttgctattg gtgtgattct ggttggcacc tgcccgggag
      421 gaactgcctc caatgttatt acgtatctgt caaagggcga tgtagcgctt tcggtagcaa
      481 tgaccactgt aagcaccttg ctgtcacctg ctttgacccc tttgttaacg tacctgcttg
      541 ccgggcggtg ggtgccggta ccaattgttt ccatggttat ttctattgta caggttatta
      601 tcgtgccggt agcactgggc attatggtgc ggcttctttt gaaaaaatat attgaccgtg
      661 tgttaatggt gctcccttca gtttcagttg tggcgatagt tactattata gccgggatca
      721 ctgctgcaaa tgctcatgcg ttgcgcacgg taggttttat taccgtgctt gtggtaatac
      781 tgcataatgt gacaggatta gccctggggt atgctgctgc ttcgttgctt ggatttgata
      841 aaagtgtgcg aaaaaccatt gctattgaag tgggtatgca gaattcaggc cttgcagtgt
      901 cacttgcaat catgcatttt gaagctctgg ctgcattgcc tggtgcaatc ttcagtatat
      961 ggcacaacat ttcgggttca attgctgcat ggtggtggcg aagacggtag atttatggag
     1021 ttgtctccca gggtcttttg caagttgtcc acatgtatgg taagctcacc agcaagcttt
     1081 ttaaatgtca ttgatatatc agttaaatta tcataaaaag tatgaatctg ttctaaagct
     1141 tgctgtaatt gttctactgc tgttgcattt ttttctgtaa gttcattaat atactgggtg
     1201 tattttaaag aatttcctaa atgcccaatg acggtgttaa tatttttatc ctgtgatgat
     1261 acaagttctt gaaactttag aaaaaatgac ctgtttgctt caagaaattg aatgatttca
     1321 atcagcgcta acgaagcatc atctatctga actgccgcac tgagaattcg ctgtactgaa
     1381 cggccaatga tgccgcttat ttctttggcc gaagactggc ttttatctgc taattttgag
     1441 atttcatcgg caactacagc aaatccccgc ccatgttcac cagcacgtgc tgattcaatt
     1501 gctgcgttaa gtgaaagcag gtttgtctga tctgaaattt cgttgatggt ttgtgatatt
     1561 tcttctatca ttttagattc gtcacggaga cgttcaattg tttgacgggc atcctgcagt
     1621 ttgcggttac agtcatcgga aagttcctga atcttttttg cttcaccact tgcttgcttc
     1681 ccggcttcag cggcgtttgt aaggtctgtg cccagacctt tagtgatgtc atttatttct
     1741 ttattatttt caatttcttt caacgtagct tctttcaata cagtaaatga ttcaataata
     1801 tctgtactga tagcacggct tttttcaaag attgcagaat gttcatgtat gatagcagta
     1861 atatctcttg aaaaattatt tataatggtt gaagctttac gaacgtgttc cactactgtt
     1921 tt
//
LOCUS       DAOSZP010000458         1913 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_45142_length_1913_cov_2.628095, whole genome shotgun sequence.
ACCESSION   DAOSZP010000458 DAOSZP010000000
VERSION     DAOSZP010000458.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45142_length_1913_cov_2.628095"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..382
                     /locus_tag="PK348_09275"
     CDS             <1..382
                     /locus_tag="PK348_09275"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HXK66446.1"
                     /translation="NQQLEKIPVTGFAYKSAVVPKQSWDKWAEVAAPVVKEILNKLPE
                     GYVLQVTGHADGSGPEEPVGNKPGNIKISTERAKAVYNALKAKGIDSPKMTYKGVGSS
                     ELLPGVDPRDAANRRVTFKVVPKK"
     gene            475..>1147
                     /gene="rsgA"
                     /locus_tag="PK348_09280"
     CDS             475..>1147
                     /gene="rsgA"
                     /locus_tag="PK348_09280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HXK66447.1"
                     /translation="MHGVVSKVMGLYYTVYHDSTYTNCVLRGKLRQKFECDTEKFSNP
                     VAVGDEVEITIADDGTGVIEEIVPRKNYFSRKEKGKHRKIDIIAANCDCVVVMQSFAL
                     PKLNLRFADRIAVRCGQQHIPLILCVNKYDLASKKDIEYIYNYYKNTDISIVMTSAKT
                     GYNIDEFKNLLYNKTSVIVGYSGVGKTSILNTIYPHLDLRVGEVSASTGKGKHTTTNV
                     TMIIVE"
     assembly_gap    1148..1157
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1158..1402
                     /locus_tag="PK348_09285"
     CDS             <1158..1402
                     /locus_tag="PK348_09285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HXK66448.1"
                     /translation="DTPGLREFGIMDIEPHLVSKYFYEFEKYAARCAFKPCTHDHEPD
                     CEVKRLVQKGVIYEERYISYINILYSLKDYYATMYS"
     gene            1399..>1913
                     /locus_tag="PK348_09290"
     CDS             1399..>1913
                     /locus_tag="PK348_09290"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXK66449.1"
                     /translation="MITAEHVRLTFNHHPVLHDVCFSVPQGLIYVILGKNGSGKTVLL
                     KTIAGLFTHFTGTIALAGIPLQEYINLTIEEKMKRNITMAYVFQKGGLFDSMNVFDNV
                     AFGLRRRNENEAIVTEKVMEALEWVGLSGSEQKLPSQLSGGMQKRVGLARALCLSPSI
                     ILYDDPTAGLDP"
BASE COUNT          619 a          317 c          416 g          551 t
ORIGIN      
        1 gaatcaacag cttgaaaaaa taccggtaac cggctttgct tataaaagtg cagttgtgcc
       61 caaacagagc tgggataaat gggcagaagt agcagctcca gtagtaaagg aaattttaaa
      121 caaattgcct gaaggctatg tattgcaggt aaccggtcat gctgatggtt caggacctga
      181 agaaccggtt ggcaacaaac caggcaacat caaaatatcc actgaaagag caaaagctgt
      241 atataatgct ctcaaagcta aaggaataga ttcacccaaa atgacataca aaggcgttgg
      301 ttcatctgaa cttctgccag gtgtggatcc aagggatgct gcaaacagac gcgttacttt
      361 taaagttgtt ccaaagaagt agtacagaca ataatttaat gttggttttg atgtatacgt
      421 gtagagccag ttttatgact ggctctacgt tattgtgatt aaggtttacc tggtatgcat
      481 ggtgtagttt ctaaagtaat ggggctttat tataccgtat atcatgattc gacatataca
      541 aactgcgtac tcagaggtaa attgcgccag aagtttgaat gtgatactga aaagttttcc
      601 aatccagttg ctgttggcga tgaggttgaa ataaccatag ctgatgatgg tacaggtgtt
      661 attgaggaga tagttccaag aaaaaattat ttttcacgca aagagaaagg taaacacaga
      721 aaaatagata tcattgccgc aaattgtgat tgtgttgttg taatgcaatc atttgcactg
      781 ccaaaattaa atttgcgatt tgctgacagg atagcagtca ggtgtggtca gcaacatatt
      841 ccacttattc tgtgtgttaa taaatatgat ttagcttcaa aaaaagatat tgaatacatt
      901 tacaattatt ataaaaatac tgatatcagt attgtaatga caagtgccaa aacaggctac
      961 aatattgatg agtttaaaaa cctattatac aataaaacat cagttattgt tggctattca
     1021 ggagttggca aaactagcat attgaatacc atctatccac atctggatct tagagtaggt
     1081 gaggtttcag caagcactgg taagggtaaa cacaccacaa ctaatgtaac catgataata
     1141 gtggaagnnn nnnnnnnttg atacccctgg actgcgtgag tttgggatta tggatattga
     1201 acctcatctg gtaagtaaat atttttatga atttgaaaaa tatgctgccc gatgcgcttt
     1261 taagccatgc acccatgacc atgaaccgga ttgcgaagtg aagcggttgg tgcaaaaagg
     1321 agtcatatac gaagaacggt atatttcata tatcaatatt ctctattcct taaaagatta
     1381 ttatgcaacg atgtactcat gataactgca gaacatgttc ggttaacatt taatcatcat
     1441 cccgtgttgc acgatgtgtg cttttctgta ccacagggct taatctatgt cattcttggg
     1501 aaaaatggca gtgggaagac agtgctgtta aaaacaattg cagggctatt tactcatttt
     1561 acaggaacta tcgccttagc tggcattccg ttacaggaat acatcaatct tactatagaa
     1621 gaaaaaatga aacgcaatat aaccatggcg tatgtgttcc agaaaggtgg gctttttgat
     1681 tccatgaatg tgtttgataa tgttgctttt ggattgcggc ggcgtaatga aaatgaggcg
     1741 atagttactg aaaaagtcat ggaagcatta gaatgggtgg gtttgtcagg atcagaacaa
     1801 aaattaccct cccagttaag tggtggtatg caaaaaagag tgggccttgc acgagcgtta
     1861 tgcctgtccc cttctataat tctgtatgat gatccaaccg cagggcttga ccc
//
LOCUS       DAOSZP010000459         1912 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_45164_length_1912_cov_3.196554, whole genome shotgun sequence.
ACCESSION   DAOSZP010000459 DAOSZP010000000
VERSION     DAOSZP010000459.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1912)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1912)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1912
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45164_length_1912_cov_3.196554"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..318)
                     /locus_tag="PK348_09295"
     CDS             complement(<1..318)
                     /locus_tag="PK348_09295"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HXK66450.1"
                     /translation="MKQSSVEERKILLDAELKKIVEVLKQEYDPEKIILFGSLLDGNI
                     HEYSDIDLLIIKKTSKRPIDRILEVAQIINPSLGVDLFVYTPEEFDTLVNEKYTFFTN
                     ILKN"
     gene            567..1160
                     /locus_tag="PK348_09300"
     CDS             567..1160
                     /locus_tag="PK348_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HXK66451.1"
                     /translation="MKKISYSLAVIIALSLFSFTGCGYNKLQMQEEEVFKAYGDLESA
                     LQRRADLIPNLVATVKGYAAHEKTTLQAVIEARAKATSVQLSPKNLSNPEAIAKFQAA
                     QGELSSALARLMVVIERYPNLKADAHFTDLMHQLEGTENRINVARQRYNEAVKEFNSS
                     IRIFPYNITNSLLLHLERKEYFKAEEGAKTAPKVSFE"
     gene            1174..1830
                     /locus_tag="PK348_09305"
     CDS             1174..1830
                     /locus_tag="PK348_09305"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HXK66452.1"
                     /translation="MKDLVSKILSQADAQCIIDAVKKAEKLTSAEIVPMIVNASYHYP
                     LTRIYASAVLSLPLAIALTYYIAPLFYFHKDNLWIFIVFFSLLFIVFKVTIARSVFLT
                     RLFLNPREVDAEVREAAITAFYKNGLYKTADKNGVLVYITLLEKKVWILADEDAHKKI
                     EPEFWETCVKHIITGIKQKQFIPALCATIETIGNRLAKEYPYKPDDVDELKNIIVGDN
                     "
BASE COUNT          565 a          392 c          352 g          603 t
ORIGIN      
        1 attttttaaa atattggtaa aaaaagtata tttttcattt acaagtgtgt caaattcttc
       61 tggagtatag acaaataaat caacccccaa tgatggattt ataatttgtg caacttcaag
      121 tatcctgtct atagggcgtt ttgaagtttt ttttattatc aataaatcaa tatcactata
      181 ttcatggatg tttccatcta acaatgaacc aaaaagtatt atcttttcag gatcatattc
      241 ctgtttaaga acttctacta ttttctttaa ttccgcatca agtaatattt ttctttcttc
      301 aacactactt tgtttcatta tttgctcatt cctacatttt taatctgaat ctatcttcaa
      361 atatattttg cattatgtat ttgttctata acgtaattat acattattat atttgcaagt
      421 ataatttttg tacggagata cccctctttt ccactgcatt ctgtgctctc tgtgggatat
      481 tttttattct tgacaccttt ttgttttaag tgtatatttt aattttacgc aaaacagcgg
      541 tttatctttc cccaccagga gggcttatga aaaaaatttc ctattcttta gcagttataa
      601 ttgcattgtc gctattttcc tttacgggat gcggatataa caaacttcag atgcaggaag
      661 aagaggtatt caaggcgtat ggcgatttag aatcggcact gcaacgaagg gctgacctta
      721 tcccaaactt agttgccact gttaagggat atgccgcgca cgaaaagact acactacagg
      781 ctgtcattga agcgcgggca aaggccactt ccgttcaact atcgcccaaa aacctgagca
      841 accctgaagc cattgcaaaa tttcaggcag cgcagggcga gctttcaagc gcactggcac
      901 gactcatggt ggtaattgag cgctacccaa atttaaaagc cgacgcgcac ttcactgacc
      961 tgatgcatca gcttgaagga accgaaaacc gcatcaatgt ggctcgccag cggtataacg
     1021 aagcggttaa ggaattcaat tcatcaatca gaatttttcc gtacaacata accaacagcc
     1081 tcctgcttca cttagagcgc aaggaatact tcaaggctga agagggcgct aaaactgctc
     1141 ccaaagtttc ttttgaatag gcgatagtta ccaatgaaag atcttgtttc taaaatacta
     1201 tcgcaggctg atgcccagtg catcattgat gccgttaaaa aagctgaaaa gctgacatca
     1261 gccgaaattg tccccatgat tgtcaatgca agctatcatt atccgcttac acgcatatat
     1321 gcatctgcgg tactgtcgct acctctggcg attgcactga cctactacat tgcgccgctt
     1381 ttctactttc acaaagacaa tctgtggata tttattgttt tttttagtct gctgtttatt
     1441 gtgttcaagg ttactatcgc cagaagcgtt tttttaacac gccttttctt aaacccgcgc
     1501 gaagttgatg cagaagtgcg tgaagctgcc ataaccgcat tttacaaaaa tggcctgtat
     1561 aaaacagctg ataaaaacgg cgtgcttgta tatataacgc ttcttgaaaa aaaggtatgg
     1621 atacttgccg atgaagatgc ccataaaaaa attgaacctg aattctggga aacatgcgta
     1681 aaacacatca ttaccggcat aaaacaaaag caatttattc ctgcgctgtg tgcaaccatt
     1741 gaaactattg gcaacaggct tgcaaaagag tatccgtata aacctgatga tgttgatgag
     1801 cttaaaaata taattgttgg tgacaattaa tgaaatgggt tttcacagta ctgtgtgcgg
     1861 tgtgtgtagg tgccactgcg tacgctcagg agttagctcc cataccgcct tt
//
LOCUS       DAOSZP010000460         1907 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_45314_length_1907_cov_3.808315, whole genome shotgun sequence.
ACCESSION   DAOSZP010000460 DAOSZP010000000
VERSION     DAOSZP010000460.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1907
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45314_length_1907_cov_3.808315"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..538)
                     /locus_tag="PK348_09310"
     CDS             complement(<1..538)
                     /locus_tag="PK348_09310"
                     /inference="COORDINATES: protein motif:HMM:NF015573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HXK66453.1"
                     /translation="MKLLLPFITISILLFVGHYLRLHIKLFQKLYLPSSLLGGIVGLM
                     VYQTAKTLSINTLTTWFDGLAMLPSFLINIVFACLFIGVSLPSFKIVLKKSIPQLSYG
                     QIVAWGQYMVGLGLFIFIISKVWNLPAMFGAVIEVGFEGGHGTASGLAPTFEMMKWPQ
                     GKDFALASATVGIISAVIA"
     gene            complement(732..1526)
                     /locus_tag="PK348_09315"
     CDS             complement(732..1526)
                     /locus_tag="PK348_09315"
                     /inference="COORDINATES: protein motif:HMM:NF018066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate decarboxylase family protein"
                     /protein_id="HXK66454.1"
                     /translation="MKTLSRKDFFTIPTETFKMSKGTVELPIYYYDYGYAHFIFRVDY
                     AAAKKKLADTAFIPCKFFGKAIALLNFFEYRDTAIGPYNEVGLSILCYPKSKKQPSVV
                     PLHILKDAKKWQVGAYVINLPVTTEIAYLAGKEVWNYPKFVTRIDFTLVNRYFKGVVY
                     DPTRNEPLVTLAGNIGVVSTPKLKNASFISHTTHNSVTLRTLTVVDTRFKIAYGFSGK
                     CTVNAQSNHSMAQNLLDLGLDGKKPLGCMYSPQAQMMLCKGEPIVS"
     gene            complement(1544..>1907)
                     /locus_tag="PK348_09320"
     CDS             complement(1544..>1907)
                     /locus_tag="PK348_09320"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXK66455.1"
                     /translation="CNHNYYMGKKLIDYKEIQYELADMLIKLESVRMTIWRSCAHFRA
                     CQSLSSIAKVYASDMAVEICKQAMAIMGDSGIHHEYGVEKALRDARLTQIYEGTNQIN
                     RYAIIEHQYSTDVILDNV"
BASE COUNT          554 a          386 c          370 g          597 t
ORIGIN      
        1 cagcaataac agcacttata atgccaactg ttgcacttgc cagtgcaaag tccttgcctt
       61 gtggccactt catcatttca aatgttggtg ccagtccgct tgctgtgcca tggccgcctt
      121 caaaacccac ttcaataaca gctccaaaca tggcaggcag gttccacact ttgctgatga
      181 taaaaataaa tagccccaat cctaccatat attgtcccca ggcaactatc tggccatacg
      241 acagttgggg gattgatttt tttaaaacta ttttaaacga tggcaatgaa acaccaataa
      301 aaagacaggc aaagactatg ttaataagga aagagggcaa catggcaagt ccatcaaacc
      361 aggtggtaag agtattgata gatagtgttt tagctgtctg atacaccatc aatccaacga
      421 tgccgcctaa taacgatgat ggcaggtata gcttctggaa aagtttaatg tgcagcctga
      481 gatagtgtcc aacaaatagc aggatactga tagttataaa cggtaacagt agtttcatgc
      541 acaccttctt gatttgtgat atggggcaat ttactcatct ttatttttta tgcaagcaag
      601 gtcaagataa attatactgt taccatgtct ttatttttat gcggcgatag ttacttgtga
      661 ttagtctgca gtgcacaacc atgtgcaggc aaaaatttat tagcagtaac tatcgcccaa
      721 aaaattgtat ttcaggatac aatgggctca cccttgcaga gcatcatttg cgcctgtggc
      781 gaatacatgc atcccagtgg ctttttgcca tctaagccta agtcaagcag attctgtgcc
      841 atgctgtgat tgctctgtgc attcactgtg cattttccag aaaagccgta ggctattttg
      901 aaacgcgtgt caaccacagt cagtgttctg agtgttacac tattatgtgt tgtatgagaa
      961 ataaaagaag cattttttag ctttggcgtt gacactactc caatgtttcc tgccaatgtt
     1021 accagaggtt cattacgagt gggatcatac acaacgcctt taaaataacg gttaacaagt
     1081 gtaaaatcta ttcgtgttac aaactttgga tagttccata cttctttgcc tgcaaggtat
     1141 gcaatctcgg ttgttactgg cagattaatt acatatgctc caacctgcca tttttttgca
     1201 tccttcagta tgtgtagtgg taccactgat ggttgctttt tgctctttgg atagcacaaa
     1261 attgaaaggc caacttcatt gtagggccca atagctgtat cacggtattc aaaaaagttt
     1321 aacaacgcta ttgcttttcc aaaaaatttg catgggataa atgcagtatc agctaatttc
     1381 ttttttgccg ctgcataatc aacacggaat ataaagtgtg cgtatccata gtcatagtaa
     1441 taaatgggaa gctcaacggt tccctttgac attttgaatg tctcagtggg tattgtaaaa
     1501 aaatctttgc gtgataatgt tttcatgcaa acctcctttt cgtttataca ttgtctagta
     1561 tcacatcagt actgtactga tgttcaataa ttgcatagcg gttaatctgg ttagtgcctt
     1621 cataaatctg ggttaagcgt gcatcgcgca gtgctttttc aacaccatac tcatggtgta
     1681 tgccactatc gcccatgatg gccatagctt gcttacaaat ttcaacagcc atatctgaag
     1741 catatacctt ggcaatagat gaaagtgatt ggcaggctct aaagtgggca caactacgcc
     1801 aaatcgtcat tctcactgat tctaatttta tgagcatatc agcaagttca tattgaattt
     1861 ctttatagtc tatgagtttt ttgcccatat agtaattgtg attacaa
//
LOCUS       DAOSZP010000461         1906 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_45342_length_1906_cov_3.609941, whole genome shotgun sequence.
ACCESSION   DAOSZP010000461 DAOSZP010000000
VERSION     DAOSZP010000461.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1906)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1906)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1906
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45342_length_1906_cov_3.609941"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..567
                     /locus_tag="PK348_09325"
     CDS             <1..567
                     /locus_tag="PK348_09325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66456.1"
                     /translation="SDISYSKVRGGLYYFFRGFDNDVLAFMLKGGYAHNAPYYLYPYN
                     IGRYAKGKSQSIPSDEDAYSMRGFAKDMFYGNRLAVGSIEYRIPLFRKDVGYRMLPLM
                     FRDMWLTPFVEYGNIWIDSTSLNDFKYSIGSELHIRITAGYWADIEGFAGIVKGYGDY
                     GELQVYFGIATGIEGALKQHTIIRQVIQ"
     gene            601..1512
                     /locus_tag="PK348_09330"
     CDS             601..1512
                     /locus_tag="PK348_09330"
                     /inference="COORDINATES: protein motif:HMM:NF014075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF115 domain-containing protein"
                     /protein_id="HXK66457.1"
                     /translation="MNTESLYQALNQGLLQERYALIKRNITKNKVLIEKWGGLQKVVA
                     LLSGKHVVVCGAGRSLNDALKVLRKYQYRKDLAIIATDMAYRPLVLAGIKPHFSISCE
                     AMPVSYYAFLPTSSCHLLAFSGVSSHTLATWKGKISFYNWMIYESPFDALWKLAGQHL
                     GYVATASIVTTQAISLALGCSIASLTMVGNDLAFKDNYYAQHTVVCQRFIMHYNRFST
                     ANSNDYAMIHRKKMYMVKRRESIYYTDHQFLAARQWLENLFTNVTIPVFDCSIPGCSE
                     NKVTKVTISKVMNTLYPDKPIKKRGRT"
     gene            1509..1700
                     /locus_tag="PK348_09335"
     CDS             1509..1700
                     /locus_tag="PK348_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004447972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FlbD family protein"
                     /protein_id="HXK66458.1"
                     /translation="MITLHKMNGEEFILNANHIETIESKPDTTITLTNERKYLVKESK
                     DEVLSRIYEYYHKVLTGFK"
BASE COUNT          613 a          299 c          417 g          577 t
ORIGIN      
        1 tcagatattt catattccaa ggtgcgtggt ggtttgtact attttttccg tggctttgac
       61 aacgatgtgt tagcctttat gctgaagggt ggttatgcac acaatgcacc gtactatctg
      121 tatccctata atatagggcg ttacgcaaaa ggaaagagtc agagcatacc atcagatgag
      181 gatgcttaca gcatgcgagg ttttgccaaa gacatgtttt atggtaacag gttggctgta
      241 ggcagtattg agtatcgcat tcctttattt cgaaaagatg ttgggtacag gatgctgccg
      301 cttatgttca gggatatgtg gttgacaccg tttgttgagt atggcaatat atggatcgat
      361 tctacatcac ttaacgattt taaatatagt attggttctg aacttcatat tcgtatcact
      421 gctggatact gggctgatat tgaagggttt gctggcattg ttaaaggata tggcgattat
      481 ggagagctac aggtgtattt tggcatagca actggcattg aaggggcact gaagcagcat
      541 actatcatac gccaggtaat acagtagcat gcaattttaa ctgatcaggt ttccattatt
      601 atgaatactg aaagccttta tcaggcatta aatcaggggt tattacagga gcgctatgcc
      661 ttaataaagc gtaatataac caaaaataaa gtattgatag aaaaatgggg cgggttgcag
      721 aaggttgtag cgcttttaag tggcaagcat gttgttgtgt gtggtgcagg caggagttta
      781 aacgatgcac ttaaagtgtt aagaaaatat caatacagga aagaccttgc tattattgca
      841 acagatatgg cgtacaggcc gttagtactg gctgggatta agccacattt ttctatatca
      901 tgcgaggcca tgcctgtttc gtattatgca tttttaccca cgtcatcctg ccatctgtta
      961 gcgttcagtg gagtaagctc acatacattg gctacctgga agggtaaaat cagcttttat
     1021 aactggatga tatatgaaag cccttttgat gcattgtgga agcttgcagg ccaacattta
     1081 ggatatgtag caacagcaag tattgtaaca acacaggcaa tatcactggc gttagggtgt
     1141 tctattgctt cgttaacaat ggtaggcaat gaccttgcat ttaaggataa ttactatgca
     1201 cagcatacgg ttgtatgcca gcgctttatc atgcattaca atagattttc aacagccaat
     1261 tcaaatgatt atgcaatgat tcatagaaag aaaatgtaca tggtcaaacg aagagagtcg
     1321 atatattata cagatcatca gtttctggct gccaggcaat ggctggaaaa cctttttaca
     1381 aacgtaacga taccagtatt tgattgcagt attcctggtt gcagtgagaa taaagtcact
     1441 aaagtaacaa ttagtaaagt tatgaacaca ttatatcctg ataaaccaat aaagaaaaga
     1501 ggcagaacat gataacatta cataaaatga atggcgagga attcattttg aatgcaaatc
     1561 acattgaaac tatagagtcg aagcctgata caaccataac actgaccaat gaaagaaaat
     1621 atctggttaa agagtcaaaa gatgaggtat tatcccgtat ttatgaatat tatcataagg
     1681 tgctcactgg gtttaaataa ttacaaaatg ataatttggg cgatagtacc tgggaaaaaa
     1741 tctggtagta ataataaata taataattaa tgaataattt taaaaattta accgaaaatt
     1801 gttaataaga atacctgtac gatttgaatg gctactttag gaggagtaac gctatatgga
     1861 tcttgctacc gttataggtt taattgttgg ttttaccaac ataatt
//
LOCUS       DAOSZP010000462         1902 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_45462_length_1902_cov_3.872225, whole genome shotgun sequence.
ACCESSION   DAOSZP010000462 DAOSZP010000000
VERSION     DAOSZP010000462.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1902)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1902)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1902
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45462_length_1902_cov_3.872225"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(118..555)
                     /locus_tag="PK348_09340"
     CDS             complement(118..555)
                     /locus_tag="PK348_09340"
                     /inference="COORDINATES: protein motif:HMM:NF024844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HXK66459.1"
                     /translation="MAIDKKYIGKQWPTMVYEVGKEKVKEFAKAIKSTNPYFLDDEFA
                     EQSKYKVIVAPPTFAVVFGAKLIEPIFFDNELNLNLAMLVHGEQEFEFYNVVKSGDVL
                     YSDAKIVNIENKEKLDVIAIEIMTRNQEKKDVCKGIYTFVVRK"
     gene            complement(573..1742)
                     /locus_tag="PK348_09345"
     CDS             complement(573..1742)
                     /locus_tag="PK348_09345"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXK66460.1"
                     /translation="MNYEFNDIQKSIKENFKKFCENEIKPHAALLDIGDEKSVEILKK
                     NIKKLGDLGYLGMIHEEEYGGTNQDLINFAIAGEEVAKACAATFLSAGASCGLCGMPI
                     RLFGSKKQKEKYLPGIISGDIIGSFGLTEPQAGSDAVAIATTAKKKGDKWILNGTKTF
                     ITNAPIADVCLVMAYNDKEAGPANGVTAFLVDKGTPGFSTGKPFEKLGYRGSPTSEVF
                     LEDCEVGEDAVLGEVGKGFIQAMQTLEFGRIGMATVSLGVAMACMEEAVKYSAERKAF
                     GKPINRYQEISFKLADMMILTDLSRLLIYKAAWAKQTNNPEAAILASCAKLFASEAAT
                     QISSWALQIHGGYGYIKEFPVERLYRDAKLGEIGEGTSEIQRILIARDLLQKYGS"
     gene            complement(1769..>1902)
                     /locus_tag="PK348_09350"
     CDS             complement(1769..>1902)
                     /locus_tag="PK348_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018085056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXK66461.1"
                     /translation="IFGGYGYIHDYPVERMLRDAKLAQIGGGTSEVQRLIISRLLSL"
BASE COUNT          533 a          454 c          278 g          637 t
ORIGIN      
        1 gattaaagtc acccgatgca ccagcatagt agatagccct gtatttatca acttcctgat
       61 ttaccttgaa actatcgcca acacttattg ttttgtgtga atccattgtt ttcataacta
      121 tttcctcacc acaaaagtat aaattccttt acatacatct ttcttttcct gatttctggt
      181 catgatttca attgcaatta catctaattt ttccttattc tcaatattta ctatctttgc
      241 atctgaatac agcacatcac ccgattttac aacattgtaa aattcaaact cctgctctcc
      301 atgtacaagc attgctaaat tgaggttaag ctcattatca aaaaatattg gttcaatgag
      361 tttagctcca aacactacag caaatgttgg aggggcaaca ataactttat actttgattg
      421 ttcagcaaat tcatcatcta aaaaatacgg gttggtgctt ttaatagctt ttgcaaattc
      481 ctttaccttt tctttgccaa cctcatagac catcgttggc cattgtttgc caatatattt
      541 tttgtcaata gccattgttt cacctcctca tattaacttc catatttctg taacaggtca
      601 cgggctatga ggatcctctg aatttcagat gtaccctcgc ctatctcacc caactttgca
      661 tcacggtata ggcgctcaac aggaaattct ttaatatacc cataaccacc atgtatctgc
      721 agtgcccagc tagaaatctg agtagctgct tcacttgcaa ataattttgc acatgaagcc
      781 aatatggcag cttcgggatt attggtttgc ttggcccacg cagctttata aatcaaaagc
      841 cgtgaaaggt cagtaagtat catcatatca gccagtttaa atgaaatttc ctgatacctg
      901 tttattggct tgccaaaagc tttacgttca gctgaatatt tcacagcctc ttccatgcat
      961 gccattgcaa cacccagtga aactgttgcc atgccaatcc ttccaaactc cagtgtttgc
     1021 attgcctgaa taaagccctt gcctacttcg cctaacactg catcttcacc aacttcacaa
     1081 tcctccagga atacttctga tgttggtgaa ccacggtatc ctaacttttc aaatggcttt
     1141 cctgttgaaa aaccaggtgt tcctttatca acaagaaatg ctgtaacacc attagcaggt
     1201 cctgcctcct tatcattata tgccatgacc aggcacacat cggcaatggg agcattggtg
     1261 ataaatgttt tggtaccgtt taaaatccat ttatcgcctt tcttttttgc agttgtggcg
     1321 atagcaaccg cgtctgatcc tgcctgcggc tcagttaacc caaagctgcc aatgatgtca
     1381 cccgatatta tccctggtaa atatttttcc ttttgctttt ttgatccaaa aagccgtata
     1441 ggcataccgc ataaaccaca gcttgcgcct gctgatagaa atgtagcagc acaggctttt
     1501 gcaacttctt cacctgcaat ggcaaaattt ataagatcct gatttgttcc tccgtattct
     1561 tcctcatgaa tcatgcctaa atagcctaaa tcacccagtt tcttaatatt cttcttcaaa
     1621 atctcaacag atttctcatc accaatatca agcaatgctg catgtggttt tatttcattt
     1681 tcacaaaatt ttttaaagtt ttcctttatt gatttttgta tatcgttaaa ttcataattc
     1741 attttttacc tcctaatatg ccaccagatt ataatgacaa caatcgtgat ataatcaatc
     1801 gttgtacttc agaagttcca ccacctatct gagctaattt tgcatcgcgc agcattcgct
     1861 ccactggata atcatgaata tatccatacc caccaaatat ct
//
LOCUS       DAOSZP010000463         1901 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_45488_length_1901_cov_4.054713, whole genome shotgun sequence.
ACCESSION   DAOSZP010000463 DAOSZP010000000
VERSION     DAOSZP010000463.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1901)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1901)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1901
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45488_length_1901_cov_4.054713"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..769
                     /locus_tag="PK348_09355"
     CDS             <1..769
                     /locus_tag="PK348_09355"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF004885.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HXK66462.1"
                     /translation="LPDHVYQALSTMHSSEKKPLAKETLHILLQNATIAATKHIPLCQ
                     DCGSVIVFLQIGTGVTIDGNPEEIINTAVADTYRSHYLRPSIVKDPLFDRTNTGTNTP
                     VFIHTDIVEGDSLAITVYIKGGGSENMSSLRMFTPTADIEQIIGYIVATVKQAGPNPC
                     PPLFLGIGIGGTADVAMLNAKKAVLQKEPHPNEEYRRLEEVILQRCNTTGVGPLGFGG
                     NNTVATVRIKEAPTHIATLPVALNLNCHSLRYRSTIL"
     gene            766..1293
                     /locus_tag="PK348_09360"
     CDS             766..1293
                     /locus_tag="PK348_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019999618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FumA C-terminus/TtdB family hydratase beta
                     subunit"
                     /protein_id="HXK66463.1"
                     /translation="MKTIDLHTPIPEHDLQQLKAGDFITISGTIYTARDKAHKKIAEL
                     VAQQKPLPIDFTNAVIYYAGPSPAPPGMPIGAIGPTTSYRMDAYTEAMLSLGVRMFIG
                     KGSRSSEVKKLLVNYNAVYCSSFGGAAAYLNQCVVDAQIVAFEEFGPEAMYKLTVEKF
                     PVIVINDMYGGDLYA"
     gene            1286..1888
                     /locus_tag="PK348_09365"
     CDS             1286..1888
                     /locus_tag="PK348_09365"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HXK66464.1"
                     /translation="MLKFSDYNEFLKFCDRIKQVLHQRPPRAIERKSLKDAAVLIPIL
                     LKDNQPHVLVTKRTNNVGTHKGEVSLPGGGIDEDDATPLDTALRETYEEVGIKPDDVT
                     ILGEFDHFISIYGFHVYTFVGIIPYPYDLTINHDEIESYTEVPLSLFIEEKYDDVKYY
                     TYEGTPYAIYYYHFNGYTIWGLTARILTDFSRKVIKNIVV"
BASE COUNT          591 a          392 c          377 g          541 t
ORIGIN      
        1 cctacctgac cacgtttatc aggcactatc taccatgcac agtagtgaaa aaaaaccttt
       61 agccaaagaa actcttcata tattgctaca aaacgccacc atcgctgcta ccaagcatat
      121 tcctctatgt caggactgtg gttcagtgat agtattttta cagataggca ccggcgttac
      181 cattgatggc aaccctgaag agataataaa tacggcagtg gcagatacct acagaagtca
      241 ctatctgcga ccatccatag taaaagatcc actatttgat agaaccaaca caggcaccaa
      301 tacaccggta ttcatccaca ccgatattgt tgaaggggat tctcttgcca ttacagtata
      361 cataaaaggt ggtggatcag aaaacatgtc ctcattaagg atgtttacac caacagcaga
      421 tattgaacaa attattggtt atatagtagc cactgttaaa caggctggtc ccaatccctg
      481 ccctccactt tttttgggaa ttggaattgg tggaactgct gatgttgcca tgcttaatgc
      541 aaaaaaggcg gttttgcaaa aagaaccaca tcccaacgaa gaataccgca ggcttgaaga
      601 ggttatatta caaaggtgca atacaactgg agttggtcct ttagggtttg gtggcaacaa
      661 tactgttgca actgtcagaa taaaagaagc tcctactcat attgcaacac ttcccgttgc
      721 acttaatctt aactgccatt cattacgcta caggtccact atactatgaa aactattgat
      781 ttacatacac ccataccaga acacgatttg caacaattga aggcagggga ctttataact
      841 atatctggca ccatctatac tgcccgtgac aaagctcata aaaagatagc agagcttgtt
      901 gcacagcaaa aacctctccc catagatttt actaatgcag taatctatta tgcagggcca
      961 agtcctgcac caccgggtat gcctatagga gctatcggcc ccacaacctc ttaccgtatg
     1021 gatgcctata ctgaagccat gctgtcgtta ggcgtcagga tgttcatagg caagggatct
     1081 cgcagcagtg aagttaaaaa gctattggtt aattataacg cggtatattg ctcaagcttt
     1141 ggaggtgctg ctgcctatct caatcaatgt gttgtggatg cgcagatagt tgcgtttgaa
     1201 gagtttggcc ccgaagcaat gtataaattg acagtagaaa aatttcctgt cattgtaatc
     1261 aatgatatgt atggcggtga tttatatgct taagttcagt gattataacg aatttttgaa
     1321 attttgtgac aggataaagc aggtattgca ccagcgccca ccccgagcaa ttgaacgcaa
     1381 aagtctgaaa gatgctgcag tgctcattcc tattctttta aaagacaatc agcctcatgt
     1441 gcttgttacc aaacgcacaa ataacgttgg gactcacaag ggtgaggttt cattaccagg
     1501 cggtggaatt gatgaagatg atgcaacccc actagataca gcgcttcgcg aaacctatga
     1561 ggaagttggc ataaagccag atgatgtgac aatattgggt gaatttgacc attttatatc
     1621 aatatatggt tttcatgtat atacttttgt tggaattata ccatatcctt atgatcttac
     1681 cattaatcac gatgaaatcg aatcatatac tgaggtgcct ctttctttat ttattgaaga
     1741 aaaatatgac gatgtcaaat attataccta tgaaggcaca ccgtacgcca tttattatta
     1801 ccatttcaat ggatatacaa tatggggtct aactgctcgc attcttactg atttttcacg
     1861 taaggtgata aaaaatattg tagtctgaat attggttatg t
//
LOCUS       DAOSZP010000464         1898 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_45580_length_1898_cov_3.289745, whole genome shotgun sequence.
ACCESSION   DAOSZP010000464 DAOSZP010000000
VERSION     DAOSZP010000464.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1898)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1898)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1898
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45580_length_1898_cov_3.289745"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..721
                     /locus_tag="PK348_09370"
     CDS             <1..721
                     /locus_tag="PK348_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014323167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HXK66465.1"
                     /translation="TTINIPLPPNTSDEGFLIAMERVVLPILKDFKPDLIINSAGQDN
                     HYSDPITNMNFSALGYAKLTEMLNADIAVLEGGYSIQGALPYVNLGITLAMAGLDYSY
                     VHEPDYSQKDLTQDKEITKYIKRVCDQVMKRYEECKKPSFKLMPGNYDVRKKEIYYDT
                     DNIREMQLESIMVCEECRGVLKIETTSTVNPLCLGIEIPLDACDRCQKEGYRLLDTAL
                     SGDRYKYVQLIDRKNKKYVLY"
     gene            complement(783..>1898)
                     /locus_tag="PK348_09375"
     CDS             complement(783..>1898)
                     /locus_tag="PK348_09375"
                     /inference="COORDINATES: protein motif:HMM:NF037084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase type III"
                     /protein_id="HXK66466.1"
                     /translation="NLGNNLLNPGKSPQDNVQFLVFLTATIRAVYYHADLLRASVASS
                     GNDHRLGANEAPPAIISVFLGEQLTKILNDIENGIITKATNEAIIDLGLSTLPVLSKD
                     NTDRNRTSPFAFTGNKFEFRAVGSSQSIAFPVTILNTIVAESLDILADKIETKSGDVR
                     QAAIDVIRDEIKKIKPILFMGDNYSREWEEEAARRGLPNKKVTVESLPDLVTKKAITL
                     FQSYNVLSPVELEARYAIRLERYIKDMDIEAKTMLDMVRTRIIPATMRYQSSVASSCL
                     RIIDLLGEKAPVDAQKKMCTTITALNNDVIKEASKLDELIEKASTIHDELEKAKFYAY
                     TIKEQMGKLRAKADMLENHTSDNLWPFPRFWEMLFIS"
BASE COUNT          554 a          380 c          372 g          592 t
ORIGIN      
        1 caccaccatt aatattccat taccacccaa cacctctgac gaaggatttt taatagcaat
       61 ggagcgtgtg gtgcttccca ttttaaaaga ttttaagcct gatcttataa ttaattccgc
      121 agggcaggac aaccattact cggaccctat caccaacatg aatttttctg cactggggta
      181 tgctaaatta actgaaatgc tcaatgctga tattgcagta cttgaaggcg ggtattccat
      241 tcagggagca ttgccatacg tcaatctggg cataacgctt gccatggcag ggctggatta
      301 ttcatatgtg catgagcctg attattcgca aaaagacctt actcaggata aggaaataac
      361 taaatacata aaacgggttt gcgatcaggt aatgaagcga tatgaagaat gtaaaaagcc
      421 ttcatttaaa cttatgccag gcaattacga tgtgcgcaaa aaagaaattt attatgacac
      481 tgataatatc cgtgagatgc agttagaatc tatcatggtg tgcgaagaat gccgtggagt
      541 gctgaaaatt gaaacaactt caaccgttaa tccattatgt ctgggtattg aaataccgct
      601 tgatgcatgt gacagatgcc agaaagaagg ataccgtttg cttgacactg cactgtcagg
      661 tgaccggtat aaatatgtgc aacttattga cagaaaaaat aaaaaatatg tgctgtatta
      721 gataattgca ggggcatatg atcatacacc cctgcaggaa atacatccat tccagcaaat
      781 ctttatgata taaacagcat ctcccagaat cgtgggaatg gccacagatt atcacttgta
      841 tgattttcca gcatatcggc tttggctcgt agtttcccca tctgttcttt tatggtatag
      901 gcatagaatt ttgctttttc aagttcatca tggatagtgc tggctttttc aatgagctca
      961 tccagcttac tggcttcttt tattacatca ttgttgagtg cggttattgt tgtacacatt
     1021 tttttctgag catcaaccgg tgccttttct cctagcagat caattatccg caaacatgat
     1081 gacgcaactg aactttgata ccgcatggtt gcaggtataa ttctggtgcg taccatatca
     1141 agcatagtct ttgcttcaat gtccatatct tttatgtagc gctcaaggcg tatagcatac
     1201 cgtgcttcaa gttcaacagg tgataataca ttatagctct gaaacaatgt tatggctttt
     1261 tttgtaacta aatcaggcaa tgactctact gttacttttt tgttaggcaa tcctcttcgt
     1321 gctgcttcct cttcccattc tcttgagtaa ttgtcgccca tgaaaagaat aggttttatt
     1381 tttttaatct catcacggat cacatcgatt gctgcctgac gtacatcacc acttttggtt
     1441 tcaattttat ctgccagtat atccaggctt tcagcaacaa tagtattcag gatagtaacc
     1501 ggaaatgcaa tagattgaga agacccaaca gccctgaatt caaacttgtt gccagtaaat
     1561 gcaaatggtg aggtacggtt acggtcagta ttatctttgc tgagtaccgg tagagttgaa
     1621 agccctaaat caataatagc ttcattggta gcttttgtta tgatgccatt ttcaatatca
     1681 ttaagtattt ttgtaagctg ctcacctaaa aacactgaga taattgccgg tggtgcttca
     1741 ttagccccta atctatgatc attgccactg gaagcaactg acgcacgtaa taaatctgca
     1801 tgatagtaca ccgcacgtat ggttgcagtt aaaaatacca aaaactgtac attgtcctga
     1861 ggtgatttcc cgggattaag caggttattg cctaagtt
//
LOCUS       DAOSZP010000465         1897 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_45611_length_1897_cov_3.379479, whole genome shotgun sequence.
ACCESSION   DAOSZP010000465 DAOSZP010000000
VERSION     DAOSZP010000465.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1897
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45611_length_1897_cov_3.379479"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            158..928
                     /locus_tag="PK348_09380"
     CDS             158..928
                     /locus_tag="PK348_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004076105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HXK66467.1"
                     /translation="MKLKLISWNVNGIRAVYKKGFFQWFVTEKPDILCLQETKATADQ
                     FPEELKNVEGYNTYFAEAQKKGYSGVALFSTVQPQKVETGFGIERFDTEGRILIAYYD
                     AFVLYNIYFPNGKASKERLQYKMDFYDAFLVHAEKLRKKGMSIVMCGDVNTAHTEIDL
                     ARPKENENVSGFLPQERAWIDTFINKGYTDTFRLFIKEGGHYSWWDYKTKARERNIGW
                     RIDYFFVSNDLVPKVKDACILDKVEGSDHCPVGIVLEI"
     gene            complement(1038..>1897)
                     /locus_tag="PK348_09385"
     CDS             complement(1038..>1897)
                     /locus_tag="PK348_09385"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXK66468.1"
                     /translation="SFKSLLNMLKKSKQIIFIKEFTMDLQLTGKVAVITAASKGLGKA
                     VALELSKEGAVCYICSRDSNILQKAADEIMSVTGNTVIALPCDVTNPDDITKCKDAIV
                     KDYKTVDILFTNAGGPPAGFISDFSSEDYIRAIELNLISAINLIYAFLPVMQKNQYGR
                     IIASTSITVKQPLDNLVLSNVSRVGVVAFIKSIANQYGKYNITANAVAPGYILTDRLK
                     SLIELKAQQDNITYEKALASFASEIPLQRIGTPQEFAALVAFLASPKSGYINGQTILI
                     DGGMYKGLF"
BASE COUNT          604 a          311 c          376 g          606 t
ORIGIN      
        1 ctattagaaa atcgttttga tgttttatca cgcaggttat tccaatttat ggtgtggacg
       61 tttgggcttg ttgtttcatc aacaggtatt attattgcat tcatgaagat gcagtaagaa
      121 aaagattatg agtagaaaaa aatagtaata cgacagtatg aagctaaaac ttatttcctg
      181 gaatgtgaat ggaatcagag cagtttataa aaaaggcttt tttcagtggt ttgtaactga
      241 aaagcctgat atcctatgcc tgcaggaaac aaaagcaact gcagaccaat tcccggaaga
      301 attgaaaaat gttgaaggat acaatacgta ttttgctgaa gcacagaaaa aaggatacag
      361 tggggttgca ttgttttcta cagtacagcc acaaaaagtg gaaacaggtt ttggcataga
      421 gcgttttgat actgaggggc gtattttgat tgcatactat gatgcctttg ttctgtacaa
      481 tatctatttc cccaatggca aagcatcaaa agaacgattg caatataaaa tggactttta
      541 cgatgctttc ttagtgcatg ctgaaaaatt gcgtaaaaag ggtatgagta ttgtaatgtg
      601 tggtgatgtt aatactgcac acaccgaaat agacctggcc cgtccaaaag aaaatgagaa
      661 tgtctcagga tttttgcccc aagaacgtgc ctggatagat acatttatta ataaaggata
      721 tactgataca ttcaggttgt ttataaaaga aggtgggcac tattcatggt gggattacaa
      781 gacaaaagct cgagagcgga atataggatg gagaattgac tacttttttg taagtaatga
      841 ccttgtgcca aaggtcaagg atgcatgtat tcttgataaa gtagaaggtt cagaccattg
      901 tcctgttggc atagtgttgg aaatttaaat aaaatactca tatatgctgt atttagatta
      961 cagtatcagc agctaaatta aaccattaaa tataaaacga atcgcaataa tagaacatag
     1021 atcttagaac ttagatctta gaataatccc ttatacattc ctccatcaat aagaatagtt
     1081 tgtccattaa tatagcctga ttttggtgag gctaaaaatg caaccagtgc agcaaattct
     1141 tgaggggtac caatacgctg caacggtatt tctgatgcaa aactggcaag ggctttttca
     1201 tacgttatgt tgtcctgctg tgctttcaat tcaatcaagc tttttaaacg gtcagtgagg
     1261 atatatccag gagcaactgc attggctgtg atgttgtatt tcccatattg gtttgcgatg
     1321 cttttaataa atgcaacaac gccaacacgc gatacatttg ataaaaccag attatccagc
     1381 ggttgcttta ctgtaataga tgttgaagca atgatcctgc catattgatt tttttgcata
     1441 acaggcaaaa aagcgtagat gaggtttatg gcacttatta aattcagttc aatagccctt
     1501 atatagtctt cagatgaaaa atcactgata aatcctgctg gtggtcctcc agcattggtg
     1561 aagagtatat ccactgtctt gtaatccttt actatggcat ctttacattt agtaatgtcg
     1621 tcaggattgg ttacatcaca cggaagtgca atgacggtgt ttccagttac tgacattatt
     1681 tcatctgcag ctttttgtaa tatgttacta tcgcgcgagc atatataaca tactgcacct
     1741 tcttttgata attccagtgc aacagcttta cccaatcctt tgcttgctgc agttattaca
     1801 gccactttgc cggttagttg taaatccatt gtaaactcct ttataaaaat aatttgtttt
     1861 gactttttta acatattcag taagctttta aatgatt
//
LOCUS       DAOSZP010000466         1895 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_45689_length_1895_cov_2.467391, whole genome shotgun sequence.
ACCESSION   DAOSZP010000466 DAOSZP010000000
VERSION     DAOSZP010000466.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1895)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1895)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1895
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45689_length_1895_cov_2.467391"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            166..501
                     /locus_tag="PK348_09390"
     CDS             166..501
                     /locus_tag="PK348_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66469.1"
                     /translation="MSTPSEAVQILVTVIPIVGIVAGSVVIFFFLYFNHKQKMLLIEK
                     GLYQKISFDFDSFSLFSGFLLTGVGGALTLFFVLKDGISYALIGGLVPLGLGISFIMY
                     YFTKMKTTK"
     gene            498..1055
                     /locus_tag="PK348_09395"
     CDS             498..1055
                     /locus_tag="PK348_09395"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXK66470.1"
                     /translation="MTAQHDEYIVQQILKGKQELFSIIVERYQNHIFTLGMRFFKNYD
                     DAADFTQEVFVKVYNNLASFKGLASFKSWLMKVGYNHAINKLNIAKTNNSDEYNDSID
                     NCPNPETQVMQNELQSILEKAISTLPQEYRICIDLYFYVGLPFKEISNITGYPVNTIK
                     SYVFRAKQQLRHALKGTIAEEYHEM"
     gene            1045..1491
                     /locus_tag="PK348_09400"
     CDS             1045..1491
                     /locus_tag="PK348_09400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66471.1"
                     /translation="MKCEKAIEKYLSLDNKKSMPLSLMIHLFACKQCRKEIEDLRSIF
                     TTLQNPPYAISLENKIMQQIMLQKSYYQKVSNFNWVAAGLIIVLSIGVISYSDTLQWL
                     SLHFGNKILVPLYLVMGCIVSGYIGSYVATHLKKLEAIAHSIKSLL"
     gene            complement(1488..>1895)
                     /locus_tag="PK348_09405"
     CDS             complement(1488..>1895)
                     /locus_tag="PK348_09405"
                     /inference="COORDINATES: protein motif:HMM:NF012688.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HXK66472.1"
                     /translation="AYPKIKDYIDSTIAFARKHGYVQTLLGRKRYIPEITSSNQFRRE
                     GAERIAINTPIQGTSADMIKVAMIAIHKAFTDKKLKSRMILQVHDELVFEVPEDEKDI
                     VEGIVVSAMKSAITLDVPVVVDVGWGKNWAQAH"
BASE COUNT          598 a          375 c          338 g          584 t
ORIGIN      
        1 tcatcttttt aaaattacag aaatattgct ggatgaatat aataaatcct tgaaagaaga
       61 agaacatact gatacatttg caccatcatt tgccagcaca ttcacaacca gtactgacac
      121 tgctgggatg gtacatattg ataatccata ggaggccatt gcatcatgtc aacaccttca
      181 gaagctgtcc agatattagt gactgtcatt cccatagttg gtatagtagc aggaagtgtt
      241 gtgatcttct ttttcctgta tttcaatcat aaacaaaaga tgcttctcat tgaaaaagga
      301 ttgtatcaga aaatatcatt tgattttgat tcattcagcc ttttcagcgg atttctacta
      361 accggagttg gtggtgcact tacccttttc tttgttctta aagatggcat atcatatgct
      421 cttatagggg gtctggttcc actgggattg ggcataagct tcatcatgta ttactttacc
      481 aaaatgaaaa caacaaaatg acagcacaac atgatgagta cattgtacaa cagattctta
      541 aaggcaagca ggaattattt tcaattattg ttgaacggta ccagaatcac atatttacac
      601 tgggaatgcg gttttttaaa aattacgatg atgcagctga ctttactcag gaggtttttg
      661 ttaaagtgta caataattta gcttcattca aagggcttgc atcatttaaa agctggctta
      721 tgaaagttgg gtacaatcat gctattaata agcttaacat agctaaaacc aataattccg
      781 atgaatataa tgatagtatt gataattgtc ccaacccgga aacgcaggta atgcagaatg
      841 aactacagtc aatacttgaa aaagccatta gcactcttcc tcaagaatac cgtatttgca
      901 ttgaccttta tttttacgtt ggtttgccat ttaaagaaat ttccaatata accggttatc
      961 ccgtaaatac aatcaagtcg tatgtcttca gggcaaagca gcaattacga catgcattaa
     1021 aaggcaccat agcggaggaa taccatgaaa tgtgaaaaag ccattgaaaa atacttaagc
     1081 ctggacaaca aaaaatccat gccattatca ttaatgatcc atctttttgc gtgtaagcaa
     1141 tgcagaaaag aaattgaaga tctgcgctct atatttacaa ccttacaaaa tccaccgtat
     1201 gccatatcat tagaaaacaa aatcatgcag cagataatgt tacaaaaatc ctactatcaa
     1261 aaggtttcca attttaactg ggttgctgcc ggcctcatta ttgtactgag cattggggtt
     1321 atatcttaca gtgatacact gcaatggtta agcctgcact ttggcaataa aatcctggtg
     1381 ccattgtatc ttgtcatggg gtgcatagta tcagggtata ttggcagtta cgttgcaaca
     1441 catctaaaaa aattagaagc gatagctcat agtattaagt cattgctcta atgagcctgt
     1501 gcccagtttt ttccccaacc tacatccaca acaaccggca catcaagagt gattgcactc
     1561 ttcattgcac tcactacaat gccttcaacg atatcctttt catcttcagg cacctcaaac
     1621 accagttcat catgaacctg aagaatcatt ctacttttta actttttatc agtaaatgct
     1681 ttgtgaatgg caatcattgc cacctttatc atatcagctg atgttccctg aatgggagtg
     1741 ttgattgcga ttcgttctgc accctcgcgc cggaactgat tgcttgatgt aatttcgggg
     1801 atgtagcgct ttcgacccag caatgtttgc acatacccat gtttccgggc aaaagcaata
     1861 gtactgtcaa tgtagtcttt aatctttgga tatgc
//
LOCUS       DAOSZP010000467         1893 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_45743_length_1893_cov_2.794342, whole genome shotgun sequence.
ACCESSION   DAOSZP010000467 DAOSZP010000000
VERSION     DAOSZP010000467.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1893)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1893)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1893
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45743_length_1893_cov_2.794342"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..523
                     /locus_tag="PK348_09410"
     CDS             <1..523
                     /locus_tag="PK348_09410"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HXK66473.1"
                     /translation="ENRVQEAKAKIPQLQGNFTFHLVGHLQSNKAKDAVRLFECIHSI
                     DKISTAIEVDKEAKKINKIQNILIEVNTSGEPSKFGVEPEKAVELCKAIRDLQNVTCT
                     GLMTVGPLTEDRNKIRDAFKQLRHLKEEINQTLGISLQELSMGMSGDFDIAIEEGATM
                     LRIGTAIFGQRIS"
     gene            523..1326
                     /gene="proC"
                     /locus_tag="PK348_09415"
     CDS             523..1326
                     /gene="proC"
                     /locus_tag="PK348_09415"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HXK66474.1"
                     /translation="MDLGNISIGCIGLGNMGSAICIGLSKLLHSENIIGYDVDSSKVK
                     ELSQKVNLSAASSIQELSDKSDVIIIAVKPNMVETVAREIKSKQKSALVISIAAGITL
                     ARLSEWLGDSYRIIRCMPNTPALIGEGMSVLSTVSATENDIHIAQDIFSALGKVIILP
                     EHLMDAVTAVSGSGPAYVFTFIQAMADGGVKAGLSRNDALTLAAQTVLGAAKMVLQGE
                     EPIILRGKVTSPGGTTIDAIHVLEKNGFSGIVMDAIEAACSKSKKLGEK"
     gene            1326..>1893
                     /locus_tag="PK348_09420"
     CDS             1326..>1893
                     /locus_tag="PK348_09420"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HXK66475.1"
                     /translation="MLTNYHIWQIKKAIRFMIPVIIAIFVIGNIIAFFIKDPIKVPDN
                     ALYKKNGLLLFKDASQYISLLEKYPYNPGVKLTIYTIREGESYWDVIQRYGISIDTFI
                     GANPQLDSLLAKAGTKIVIPHKDGLLIACKHFYEAWLAKLYTSGKGSISGRYIHHPLE
                     LLAMDDIRFAFIENARPVAVNSKIEKLYA"
BASE COUNT          624 a          347 c          394 g          528 t
ORIGIN      
        1 cgaaaaccga gttcaggaag caaaggcaaa aattccacag ttacagggca attttacctt
       61 tcaccttgtt ggtcacctgc aatcaaataa agcaaaagat gccgttaggc tatttgagtg
      121 cattcactca attgacaaga tttcaactgc aattgaggtt gacaaagaag ctaaaaaaat
      181 taataaaata caaaatatcc ttattgaagt aaatacgtca ggtgaaccat caaaatttgg
      241 agttgaacct gaaaaagcgg ttgaactttg caaggccata cgtgacctgc aaaacgttac
      301 ctgcacaggc ctcatgacgg tagggccttt gacagaggat cgcaataaaa tacgtgatgc
      361 atttaaacaa ttgagacatt taaaggaaga aataaaccaa acattaggca tttcattaca
      421 ggaactatcg atgggaatgt caggagactt tgatattgcc attgaggaag gtgccacaat
      481 gctgcgtatt ggcacggcaa tttttggaca gaggatatcg tgatggatct gggaaatata
      541 tcaattggtt gtattggcct gggtaacatg ggaagtgcaa tatgcattgg cctgtcaaag
      601 ttattacatt cagaaaacat tattggctat gatgtcgata gttctaaagt aaaagaactt
      661 tcacaaaaag ttaatctttc agctgccagt tcaattcagg aactatcgga caaaagcgat
      721 gttattataa ttgccgtaaa accaaacatg gttgaaacag tagcccgtga aattaaaagt
      781 aaacaaaaat ctgcactggt tatttcaatt gctgcaggta ttactcttgc caggctttct
      841 gaatggctgg gcgatagtta ccgtataata cgctgtatgc ccaatacgcc tgcgctcatt
      901 ggcgaaggca tgtcagtact atcaaccgtt tctgcaaccg aaaatgacat acatattgcg
      961 caggatattt tcagcgcatt aggtaaagta ataatattgc ccgaacacct tatggatgct
     1021 gttactgcag tatcaggcag tggacctgcg tatgtcttta catttataca ggcaatggcc
     1081 gatggaggtg ttaaagcagg actttcacgt aacgacgccc tcacgttagc tgcgcaaact
     1141 gtactgggtg cagcaaaaat ggttttgcag ggcgaggagc caataattct gcggggtaaa
     1201 gtaacctcac ctggtggtac caccattgat gctattcatg tactggaaaa aaacggattt
     1261 tcaggtattg tcatggatgc tattgaggct gcctgcagta aatcaaaaaa attgggggaa
     1321 aagtaatgct tacaaactac catatatggc agataaaaaa agcaatcagg ttcatgatac
     1381 cagtaatcat tgctattttt gttataggaa atattatagc tttctttata aaagatccca
     1441 tcaaagtgcc tgataatgcg ctgtataaaa aaaatggatt acttcttttt aaggacgcat
     1501 ctcaatatat atcacttctt gaaaaatatc catataaccc tggtgttaaa cttacaattt
     1561 atactatccg tgaaggcgaa tcgtactggg atgttattca acggtatggt atcagtattg
     1621 atacctttat tggcgccaac cctcagttag attcattact tgcaaaggct ggtacaaaaa
     1681 ttgtcatccc tcacaaagat ggcctactta ttgcctgtaa acatttctat gaggcatggc
     1741 ttgctaaact gtatacaagt ggtaaaggtt caataagcgg cagatacata catcacccac
     1801 tggaattatt agcaatggat gatatacgat ttgcattcat tgagaatgcc cgtcctgtag
     1861 cagtaaacag taaaattgaa aagttatatg cct
//
LOCUS       DAOSZP010000468         1885 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_45973_length_1885_cov_3.551366, whole genome shotgun sequence.
ACCESSION   DAOSZP010000468 DAOSZP010000000
VERSION     DAOSZP010000468.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1885)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1885)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1885
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45973_length_1885_cov_3.551366"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..695
                     /locus_tag="PK348_09425"
     CDS             <1..695
                     /locus_tag="PK348_09425"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HXK66476.1"
                     /translation="SFIQRSFGFETAFSIAVDAIYAAHTEAEGAPNGIGLVKLMGRHS
                     GFIAANAALASNDVNFVLLPEVPFEIDGPNGLCAHLEERLKRRGHAVICVAEGAGQEI
                     LQKDSEKKYDASGNVKLADIGVYLKEKIQEYFDKKGIEINLKYIDPSYMIRSAPAVPN
                     DSIYCTLLGQNAVHAGMAGKTDLVIGQWSNIYTHVPIEVAISQRKRIDPHSRFYSSVL
                     DATGQPSSMINE"
     gene            692..838
                     /locus_tag="PK348_09430"
     CDS             692..838
                     /locus_tag="PK348_09430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66477.1"
                     /translation="MMGHEEILITSVALFYTLDPQLLKNLLSTPDVQQNSKEDVRKGI
                     HAIQ"
     gene            complement(843..>1885)
                     /locus_tag="PK348_09435"
     CDS             complement(843..>1885)
                     /locus_tag="PK348_09435"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HXK66478.1"
                     /translation="NLVIFTPTHDSYFEVPSLSKVYYALKPRPHFVIMAKRDFLSGNY
                     LSSNFGKKNKLAYYLLYFLDRTGLPKAFFKKMKLIDIPRPFIEDFNNKEAMKSEIYNQ
                     MEKLKSYIHSGFSTLIFPEGTTWGFGGLKNLRSGIFQLVDSTYRHFQKKLYILPINVK
                     VDRCMTGKKDIFINVGKPVFILDEKDKFLAKVQEILKDLHTITFSQIASVYLQRLSMA
                     VEEIDRVIVLKKEEITSHLDSIIADIMALVKEGRLPNIDEYLNDRSYRHKKLKRFLKF
                     CIKKGYIARSNGTYIINTKKVLASFPIKSYRKENPLGFSANELLSINYNVLIAVFDKY
                     CNSFRSIPTHVM"
BASE COUNT          590 a          324 c          373 g          598 t
ORIGIN      
        1 taagctttat tcagcgttca tttggctttg aaacagcatt ttctattgca gtggatgcaa
       61 tttatgctgc ccataccgaa gcagaagggg caccaaatgg aattggcctt gtaaaactta
      121 tgggacgcca ttcaggattt attgcagcaa atgcagcact tgcaagcaat gatgttaact
      181 ttgtactgtt gcctgaagtc ccctttgaaa ttgacggacc taatggtttg tgtgcgcatc
      241 ttgaagagcg cttaaaacga agaggtcatg cggttatctg tgtggcggaa ggtgctggac
      301 aggaaatttt acaaaaagat tcagaaaaaa agtatgatgc atcgggtaat gttaaattgg
      361 ctgatattgg agtatatctt aaagagaaaa tacaggaata ttttgacaaa aagggaattg
      421 aaattaatct aaagtatatt gatccaagct acatgatacg aagtgcacct gctgttccca
      481 atgattcaat ctattgcacg ctactggggc agaatgcagt tcatgcaggc atggcaggga
      541 agactgattt agtcattggt cagtggagca atatctatac tcatgttcct attgaggttg
      601 ccatatcaca gcgtaagcgt attgatcctc attcacggtt ttacagtagc gtactagatg
      661 caacaggaca gccttcttca atgataaatg aatgatgggg catgaagaaa tcctgattac
      721 atcggttgca ctgttttata cccttgatcc gcaactttta aaaaatttgc tatcaacacc
      781 agatgttcag cagaatagta aagaagatgt tagaaaagga atacatgcaa ttcagtagca
      841 gcttacataa catgtgttgg aatagacctg aagctattac aatatttatc gaatactgca
      901 attaacacat tgtaattaat tgataataat tcatttgccg aaaaaccaag aggattttcc
      961 ttgcggtatg atttaatggg gaatgatgcc agtacttttt tggtattaat gatatacgtg
     1021 ccattgcttc tggcaatgta tcctttttta atacaaaatt ttaaaaaccg ttttaatttt
     1081 ttgtggcgat aactcctgtc attaagatat tcatcaatat taggaagcct tccttctttc
     1141 accagagcca taatatcggc gattatagaa tcaagatgtg atgtaatctc ttctttttta
     1201 agaacaatta ccctgtcaat ttcctcaaca gccatagaca aacgctgcag atacactgac
     1261 gcaatctggc tgaaggtaat ggtatgtagg tcctttaata tttcctgtac cttagctaaa
     1321 aatttatcct tttcatcaag aatgaaaact ggtttgccaa cattaataaa aatatctttt
     1381 ttccctgtca tacatctgtc aactttgacg ttgatgggaa gaatatagag tttcttttga
     1441 aaatgtcggt aggtgctgtc aacaagctgg aaaataccac ttcgaagatt ctttaaaccg
     1501 ccaaagcccc atgtggtgcc ttcagggaaa ataagtgtgg aaaacccact gtgaatgtaa
     1561 cttttaagtt tttccatctg gttgtatatt tcagacttca ttgcttcttt gttattgaaa
     1621 tcttctataa atgggcgcgg aatatcaatc agtttcattt ttttaaaaaa tgctttggga
     1681 agccctgtcc tatccagaaa atacaataga taataggcta atttgttttt tttaccaaaa
     1741 tttgatgaca gatagttacc gcttaaaaaa tcccttttgg ccatgataac aaagtgcgga
     1801 cgcggtttta atgcataata tacctttgaa agcgatggga cttcaaagta actgtcatgg
     1861 gttggggtga atattactaa attgg
//
LOCUS       DAOSZP010000469         1885 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_45984_length_1885_cov_2.790710, whole genome shotgun sequence.
ACCESSION   DAOSZP010000469 DAOSZP010000000
VERSION     DAOSZP010000469.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1885)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1885)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1885
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45984_length_1885_cov_2.790710"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..773
                     /locus_tag="PK348_09440"
     CDS             <1..773
                     /locus_tag="PK348_09440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66479.1"
                     /translation="IGVNDIVKKFVPDREDTFYLKWARTISLAAAVIGVALVPLFMQF
                     KSIYLAHGTFIAAVTPPMIVTILFGILWKRYTTKAAIYTLVIGSIAMLISIQWPSVLT
                     LLSHGVAPEGLQYLRALYGLIVCTAVSIVITVLSKEKELSNIPGLVVDTIHEGKVLFK
                     GGEPDDFDEGKIIHCYIAETSEGVSLSTKMMEYLQAKPGDILYIADERWWLGGLRSIH
                     AKAGTPHQGVDNVLFLGSDLITQGNLDINRKVTVIKII"
     gene            complement(781..1359)
                     /locus_tag="PK348_09445"
     CDS             complement(781..1359)
                     /locus_tag="PK348_09445"
                     /inference="COORDINATES: protein motif:HMM:NF003191.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HXK66480.1"
                     /translation="MTDIVNTFISNYGTLFLIFVARIFDVSIGTMRIILMARGYRSIA
                     PVLGFFEVLIWLVAINSALKHFDGAISYIVYAAGFATGNYVGMLLEEKIAIGYQSVRI
                     ITSKVVSALPLVLRQEGFGVTQIDGIGMKGPITLLFAVVPKKDVKRVVEIVHLLEPNA
                     FITIEDVKQHLSGFSVHNGFAQKFAAFITKKK"
     gene            1600..>1885
                     /locus_tag="PK348_09450"
     CDS             1600..>1885
                     /locus_tag="PK348_09450"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HXK66481.1"
                     /translation="MSGYLIGILAYIIFQLILGIIVSRKIHSDDDFILAGRTLGYLLV
                     TFSVFATWFGAESCIGTSGAAYQDGLVGVTADPFGYAIVLFVLGLFFASRL"
BASE COUNT          586 a          351 c          336 g          612 t
ORIGIN      
        1 caattggtgt taatgatatt gttaaaaaat ttgtccctga tagggaagat acgttttacc
       61 taaaatgggc tcgcaccatt tctttagctg ctgcagtcat cggtgtagca ctggtaccct
      121 tattcatgca attcaaatcc atttacctgg ctcatggtac atttattgca gcagtaactc
      181 ctccaatgat agtaactatc ttatttggca ttttatggaa acgatacact acaaaagctg
      241 ctatctatac tctggttatt ggcagtatag caatgcttat ctccatacaa tggccatcag
      301 tattaacatt gttatcccac ggtgttgcac ccgaagggct acagtattta cgagcgctat
      361 atggattaat agtttgtacc gctgtcagta ttgttattac agtactgtca aaagaaaaag
      421 aattatctaa cattcccggt cttgtcgttg ataccattca tgaaggaaag gttttattta
      481 aaggcggcga acctgatgat tttgatgaag gtaaaattat ccattgctac atagccgaga
      541 ccagtgaagg tgtttcttta agtacaaaga tgatggaata tctgcaggct aaacctggtg
      601 atattcttta tatagccgat gaacgctggt ggttaggagg attacgatcc atccatgcca
      661 aagccgggac gcctcatcag ggagttgata acgtactatt tttagggtcg gatcttatta
      721 cacagggaaa tttagatata aacaggaaag taactgtcat aaaaattatc taattatata
      781 ttattttttc tttgtaataa aagctgcaaa tttttgagca aatccattat gaacagaaaa
      841 accgctaaga tgctgtttaa catcttcaat ggtaataaat gcatttggtt ccagcaggtg
      901 tacaatttca acaactcttt tcacatcttt ctttggcaca actgcaaaca ataaggtgat
      961 aggacctttc atgccaattc catctatctg agtaacacca aatccttcct gccgtagcac
     1021 cagcggcaat gcagaaacaa ctttactggt aattattcgt actgattggt aaccaatggc
     1081 aattttttct tcaaggagca tcccaacata attaccggta gcaaatcctg cagcatacac
     1141 aatataactg attgcaccat caaaatgctt caaagccgaa ttaatagcca caagccatat
     1201 taaaacttca aaaaatccca gaaccggagc aattgaccgg tatccacgag ccattaaaat
     1261 aatgcgcatg gtacctatgg atacgtcaaa aatacgggca acaaatatta aaaacaatgt
     1321 cccgtaattt gaaataaatg tattaacaat atctgtcata aattccatgg taaaaaaata
     1381 ttgtttgata gttttttttc aataactatt ctttaaaatc agtgtcttat gaatatacgc
     1441 attcaataaa tagacaacat gtgtatcaca taactttttt tgtaaaaaat gttttatgta
     1501 agattatctt tagttaattt ttttagtata tttcttgaaa aaatatatat actattctat
     1561 gtcaattgta tttttcaagt acctacggag tgaaggaagg tgtcaggata tctcattgga
     1621 attttagcat atattatatt ccagcttatc ctggggataa tagtatcccg taagatccat
     1681 tccgatgatg attttatcct tgcaggcaga acattaggct accttctggt aacattttca
     1741 gtctttgcaa catggtttgg tgccgaatcc tgcattggca cctcaggtgc ggcatatcag
     1801 gacggccttg ttggggttac tgctgatcct tttggatatg cgatagttct ctttgtactt
     1861 ggcctttttt ttgcatcccg cctgt
//
LOCUS       DAOSZP010000470         1875 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_46301_length_1875_cov_2.878022, whole genome shotgun sequence.
ACCESSION   DAOSZP010000470 DAOSZP010000000
VERSION     DAOSZP010000470.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1875)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1875)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1875
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46301_length_1875_cov_2.878022"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1739
                     /locus_tag="PK348_09455"
     CDS             <1..1739
                     /locus_tag="PK348_09455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66482.1"
                     /translation="MFRSDPNIPIYIFNCASENLVKLLDRIESVHTEIDSELEYLFGT
                     NGFIPYLIDKQKIKTWVASGRVSDEFLKGFVSIANKIHEFYREISGELLSEKYDSFKK
                     LSNFVQAIKEQPKTAEEAPRPSRIPQNLSQLYKNSIYQIFEFGLIEKENQKNLLKLLS
                     EFKKMKDPLSSEIDARKIRRQIARFYYDIYAKVFVRSQSESAVPQPVKLMLRFGFFDE
                     TMIEQEQLAELHEISMRRTEQTDIPILYEDEFLTKIFNGEENPSINEMGLSYEAHLRE
                     EQKHIKGSNAKDESDNVQKVIYEINHRIATASSVCSGSTATSFPILTSMNMRGSISHY
                     FVSKKKLESIVNALRDVDYSVFYRETAVMIGNTKELIKEEVVPYFILLPSFGTKTMLW
                     QELDGTNRRTRGRIVVPILFMGDMMPTMAHTFATFRWELNRTIMGGMWADPIEGGLTG
                     AYFDYINTFKKNPKLSLEAKQKVVERFKSIRNNRDRFADDYIQWVLYEKDGIMKLNNV
                     VRDMFYRHIPFKKELRDRLENMPAYTEIANRFRNVFNREVGNYERKFKKYQRDDGTLP
                     EALQKFMEFLYK"
BASE COUNT          649 a          305 c          388 g          533 t
ORIGIN      
        1 caatgtttag aagcgaccct aatattccaa tctatatatt caactgtgca tcagaaaacc
       61 ttgttaaact tttagatcgt attgaatcag tgcataccga aatagactcc gaacttgaat
      121 atctttttgg caccaatggt tttattccgt atttaattga taaacaaaag ataaaaacat
      181 gggttgcatc tggcagagta agtgatgaat tcctgaaagg gtttgtgtca attgccaaca
      241 aaatccatga attttaccgg gaaatatcag gtgagttgct ttcggaaaaa tatgatagtt
      301 tcaaaaagct cagtaatttt gtacaggcta ttaaagaaca gccaaaaacc gcagaagaag
      361 caccaaggcc ttcacgaatc cctcagaatt tgagccagct ttataaaaat tctatatatc
      421 aaatttttga gtttggtttg atagaaaaag aaaaccagaa aaatcttttg aagttattat
      481 cagaatttaa aaaaatgaag gaccctcttt catcagaaat tgatgcgcgt aagatacgaa
      541 ggcagatagc acggttttat tatgatattt atgcaaaggt ttttgtacgc agtcaatcag
      601 aatctgctgt gcctcaaccg gttaagctaa tgctccggtt tggttttttt gatgaaacta
      661 tgattgagca ggaacagcta gctgagttac atgaaatctc aatgcggagg acagagcaaa
      721 cagatattcc aatattatat gaagatgaat ttttaacaaa aatattcaat ggggaagaaa
      781 atcccagtat taatgaaatg ggtttatcct atgaagctca tctgcgggaa gaacaaaaac
      841 acatcaaagg cagtaatgca aaagatgaat cagataatgt gcagaaagtc atttatgaaa
      901 taaatcaccg tattgcaact gcttcttcgg tttgttctgg ttctacagca acatcattcc
      961 caatattgac atcgatgaat atgcgtggca gcatatcaca ttactttgta tctaaaaaga
     1021 aactggaatc tattgttaat gcattgaggg atgttgatta ttctgttttt tatcgtgaaa
     1081 cagcagtaat gattggaaac accaaggagc ttataaaaga agaagtagtc ccttatttta
     1141 tacttttgcc atccttcggc acaaaaacaa tgctatggca ggaactggat gggacaaaca
     1201 gaagaacacg gggcaggata gtggttccta tattgtttat gggggacatg atgcccacaa
     1261 tggcacatac gtttgccaca ttccggtggg agcttaaccg taccattatg ggaggaatgt
     1321 gggctgaccc aatagaaggt gggttgaccg gggcttactt tgattatatc aatacattta
     1381 aaaagaaccc caagctatca cttgaagcca aacagaaggt tgttgaacgt ttcaagagta
     1441 tacgtaataa ccgcgacaga tttgcagatg actatataca gtgggttctc tatgaaaaag
     1501 atggcattat gaagttaaat aatgttgtaa gagatatgtt ttatcgtcac ataccattca
     1561 aaaaagaatt gcgagacagg ctggaaaata tgcctgcata tacagaaata gccaacagat
     1621 tcaggaatgt ttttaatcgt gaagtgggaa attatgaacg aaaatttaaa aaataccagc
     1681 gtgatgatgg gacattgcct gaggctttgc aaaagttcat ggagtttctg tataaataat
     1741 agttatgaac tcacttcgca ttacaggtat aatacggcat tacacacgga aagcacgcga
     1801 ccagatttat aatgcagggt tgacaatatt taaagcagca ggcgttgaac agtatctgga
     1861 tgatattggt gcctg
//
LOCUS       DAOSZP010000471         1869 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_46489_length_1869_cov_3.013230, whole genome shotgun sequence.
ACCESSION   DAOSZP010000471 DAOSZP010000000
VERSION     DAOSZP010000471.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1869
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46489_length_1869_cov_3.013230"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1869
                     /locus_tag="PK348_09460"
     CDS             <1..>1869
                     /locus_tag="PK348_09460"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HXK66483.1"
                     /translation="QGVFPSWVHPVSKGRLCVRGWNIHELLGTQERIIKPYIKDSGQA
                     NYQDAIARLVSALSSYKPEEIAFYASPRSSNEDNFALMKLARLVFKTNNISLQAESGH
                     RNSLDVLYNGTGFAGMLGSLEDIRKAEVLIVAGMDITTQNPIIGSELHYAARSGSLLI
                     TIDSRMTKIAKLSKKFINIKPGTMRIVFDAIAHYIDKQQWSDTKSAHYEEYKKYLATV
                     PFDSIEKETGVLPAEIAFVAERLVKAKSAAVFFTSGISGLSYDTVASIYNLFALAGKI
                     GKEGCGVNPIAGINNLQGSFDMGCAPDLLTGFQSINDATAKKNFEKKWGQELPQFKGK
                     TVEQLLADSSSPLKALVIVDHDDGIVKYADRLKALPFVAYIGAFTNKVADFAHVVLPI
                     TTYIETDGTFTNAERRVQLSVQKCDNAFDVLPMWKLCDTIAQAAGKPFNYQSASEVMD
                     EIASLTPSYEGISYKLLSKKHGVQWPCTKKNHDGTKYCVPEKVSFVLPQKPYAAVQAT
                     QEYPYLLMLGKAQHYWHQNNIMHKTFIPLREYNATLLLYPDGYVALAPEDAKKIGVRD
                     RWPVKVVAEKASMKAMVMISDDVAPGTAYVPYFIREGIASFLLAYSDVVSQGEEAT"
BASE COUNT          563 a          340 c          438 g          528 t
ORIGIN      
        1 acagggagtt ttcccatcgt gggtacatcc agtaagtaaa ggcaggttat gtgtacgggg
       61 atggaatatc catgagttat tgggtacaca ggaacggatt atcaagccgt atatcaagga
      121 tagtggccag gctaattatc aggatgctat tgccaggctt gtttcggcat tatcatcgta
      181 taaaccggaa gagattgcgt tttatgcttc gccacgttcg tccaatgagg acaattttgc
      241 attaatgaaa ttggcccgtt tagtttttaa gacaaacaat atcagccttc aagcagaatc
      301 aggccatcga aatagtttag atgttctgta taatggtacg ggatttgcag gtatgttggg
      361 atcgcttgaa gatatacgga aagctgaagt gctcattgtg gcagggatgg atataaccac
      421 acaaaatccc atcattggca gtgagttaca ttatgctgct cgcagtggct cattacttat
      481 aactatcgac agccgcatga caaagattgc aaagctttct aaaaagttta ttaatataaa
      541 accaggaaca atgcggatag tttttgatgc aattgcgcat tatatagata aacaacaatg
      601 gagcgataca aaatcggcac attatgaaga atacaaaaaa tatttagcca cagtaccgtt
      661 tgattcaata gaaaaggaaa caggtgtatt gccagctgag atagcttttg tagctgagcg
      721 gctggtaaag gctaaaagtg ctgcggtatt ttttacctca ggtatttctg gattaagcta
      781 tgatactgtt gcatcaattt ataacctctt tgcacttgcg ggcaagattg gtaaagaagg
      841 ttgtggagta aatcctattg ctggtataaa caatttacag ggtagctttg atatgggatg
      901 cgctccggat ttactgacag gttttcagag tattaatgat gcaacagcta aaaagaattt
      961 tgaaaaaaaa tgggggcagg agttacctca atttaaagga aagaccgttg aacaacttct
     1021 ggctgacagt tcatcaccac tcaaagctct ggtgattgtt gaccatgatg atggcattgt
     1081 caaatatgcc gatcgtttga aagcattgcc gtttgttgcc tatattggtg catttaccaa
     1141 taaggttgca gattttgctc atgttgtatt gcccattaca acatatattg aaaccgatgg
     1201 tacatttacc aatgctgagc ggcgtgttca gctttcagta caaaaatgcg ataatgcatt
     1261 tgatgtattg cccatgtgga agctgtgtga cacgattgcc caggctgccg ggaagccatt
     1321 taattaccag agtgcatctg aagtaatgga tgagattgct tccctcactc cttcgtatga
     1381 aggtataagc tataaactgc tttccaaaaa gcatggtgtg cagtggccgt gcaccaaaaa
     1441 aaatcatgat ggcacaaaat actgtgttcc tgaaaaagtc tcatttgtac tcccacaaaa
     1501 accgtatgcg gcggtgcagg caacacagga gtatccgtac cttcttatgc ttgggaaagc
     1561 acagcattac tggcaccaga ataacatcat gcataagacc tttattccac tgcgtgaata
     1621 taatgccacg ttgctcttgt atcctgacgg atatgtggct ttagctccgg aagatgcaaa
     1681 gaagatagga gtgcgtgatc gctggccagt aaaggttgta gccgaaaagg caagcatgaa
     1741 agcgatggtt atgatttccg atgatgtggc gccaggtacc gcatatgttc cctattttat
     1801 cagagaggga attgcatcat ttttattagc atatagtgac gttgtgtcac agggcgaaga
     1861 agcaacaat
//
LOCUS       DAOSZP010000472         1866 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_46604_length_1866_cov_3.098288, whole genome shotgun sequence.
ACCESSION   DAOSZP010000472 DAOSZP010000000
VERSION     DAOSZP010000472.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1866
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46604_length_1866_cov_3.098288"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..331
                     /locus_tag="PK348_09465"
     CDS             <1..331
                     /locus_tag="PK348_09465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66484.1"
                     /translation="IVGSKVFAYRAAEAVYNLGKMEKSVFDDFKKLLYIHQKEFLDRL
                     IELMNRYKKPILPVSLSKMPGDETIHSDSGNYKVVIYDSPEEAVLCLSKMYQYYNYVE
                     KRSRVYA"
     gene            324..998
                     /locus_tag="PK348_09470"
     CDS             324..998
                     /locus_tag="PK348_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013257934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HXK66485.1"
                     /translation="MREILERAIANKQKALSEFDSKSVIKIAGIPVSRNYLAKSREEA
                     IAFAQELGFPVVLKGCSDTLTHKTELGMVKLKLKTEEEVARAYDEITGKGVPLDGVLV
                     QEWLDGDNREFVLGTIRDPQFGPCVMFGLGGVFTEALQDVSFRVAPITELDAYEMMDE
                     LRTKKLLGPFRGSPAVDRESLAKALVGLSELVSKYEEIAEVDINPVIIDGNKPVAVDA
                     LIVLKS"
     gene            1085..>1866
                     /locus_tag="PK348_09475"
     CDS             1085..>1866
                     /locus_tag="PK348_09475"
                     /inference="COORDINATES: protein motif:HMM:NF012585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HXK66486.1"
                     /translation="MKLRPAYKKIGMLFSGGPAPSANTVISSVALNFLDNGIPVIGFY
                     RGYEFLQDFNINYPKIKKGVHYEELDYNITHVRNERGIYLKTSRANPGKDIKALDDLQ
                     NAEKNKKLKNILDAFEYLKIGALISIGGDDTLKTANFLNLMGYPVIHIPKTIDNDYYG
                     IPWTFGYWTAVDTAQKMMLNLKADAQATDSFFIVELMGRKAGWITYAAGIAAEAIMMV
                     AAEDIDEDVLDIEVLVEKITNVILARENNRRPYGVIAVSEGIV"
BASE COUNT          610 a          279 c          420 g          557 t
ORIGIN      
        1 cattgttggc tcaaaagttt ttgcttatcg tgcagcagaa gcagtataca atttaggaaa
       61 aatggaaaaa agtgtttttg atgattttaa aaaattatta tatattcatc agaaggaatt
      121 tcttgacaga ttaatagagt taatgaatcg ttataaaaag cccattttac ctgtttcatt
      181 gtcaaagatg ccaggcgatg aaacaattca ctctgatagc ggtaattata aagtggttat
      241 ttatgattct cctgaagagg cagtgttatg tttatcaaaa atgtatcaat attacaatta
      301 tgtagagaag aggagtaggg tatatgcgtg agattttaga gcgtgcaatt gctaataaac
      361 aaaaagcatt atccgagttt gactcaaaaa gtgttattaa gatagcaggt attcctgttt
      421 caagaaacta tcttgcaaaa agtcgtgaag aagccattgc atttgcacag gagttaggat
      481 ttcctgttgt gttaaaaggg tgttccgata cattaacaca taaaaccgaa ctgggaatgg
      541 taaaattaaa attaaaaact gaagaagagg tggcacgggc atatgatgaa ataactggaa
      601 aaggtgttcc gttagacgga gttctggtac aggaatggct tgatggtgac aaccgtgaat
      661 ttgtgttagg aaccattcgc gatccacagt ttggtccctg tgttatgttt ggattgggtg
      721 gagtgttcac tgaggccctg caggatgtat cattcagagt tgcaccaatt accgaacttg
      781 atgcctatga aatgatggat gaactccgca caaaaaaatt attaggacct ttcaggggtt
      841 ctccagctgt tgacagggaa tcactggcaa aggcgctggt agggttaagt gaacttgtca
      901 gcaaatatga agaaattgcc gaagttgata taaatcctgt tattattgat ggcaataaac
      961 cagttgctgt tgatgcattg attgtgttga aatcataaaa aaaaataatt gctattacaa
     1021 atatctacaa gtattattta actgggattg tttggtattg aatttattgt aagaggattg
     1081 tattatgaaa ttgcgtcctg cgtataaaaa gataggtatg cttttttccg gaggtccggc
     1141 cccaagcgcc aatacagtta tatcatcagt tgcattgaat tttttggata acggtatacc
     1201 cgtaattgga ttttacaggg gctatgaatt tttgcaggat tttaacatta attatccaaa
     1261 aataaagaaa ggtgtccact acgaggagct ggattacaat attactcatg tccgcaacga
     1321 aagaggtata taccttaaaa cttcccgtgc aaatcctgga aaagatataa aggcgcttga
     1381 cgatttgcaa aatgctgaga aaaataaaaa actaaaaaat atactggatg catttgaata
     1441 cctgaaaata ggtgcgctaa tatccattgg tggtgatgat acgttaaaga cagcaaactt
     1501 ccttaatctt atgggttatc ctgttatcca tatacctaaa accattgata atgactatta
     1561 tggaatacca tggacatttg gatactggac tgcagtggat actgcacaga agatgatgtt
     1621 aaacctcaaa gctgatgctc aggcaaccga tagctttttt attgttgaac ttatggggcg
     1681 caaagcaggc tggattacct atgctgcggg tatagcagcg gaggcaatta tgatggttgc
     1741 agctgaggat attgatgagg atgtgcttga tatcgaggtg cttgtagaaa agataaccaa
     1801 cgttatactg gcacgtgaaa acaatcgccg cccttacggg gtcattgcag tttcagaagg
     1861 aattgt
//
LOCUS       DAOSZP010000473         1866 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_46605_length_1866_cov_2.957482, whole genome shotgun sequence.
ACCESSION   DAOSZP010000473 DAOSZP010000000
VERSION     DAOSZP010000473.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1866
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46605_length_1866_cov_2.957482"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="PK348_09480"
     CDS             <1..318
                     /locus_tag="PK348_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HXK66487.1"
                     /translation="YRVVLLCSADTSAASSKTYDIEVWMPGLNRYVEISSCSNFVDYQ
                     ARRARIRYRKKEGEKPVFVHTLNGSGLAAGRTLAAVMENYQTKDGTITIPDVLKPYLE
                     KTL"
     gene            319..1275
                     /locus_tag="PK348_09485"
     CDS             319..1275
                     /locus_tag="PK348_09485"
                     /inference="COORDINATES: protein motif:HMM:NF013317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HXK66488.1"
                     /translation="MLQVHTTTFAAARQALALALVKQKECSISIDPLVQQHIHYNRTL
                     QAIVEVLHQLNISCTYHNGIITVLPQKLPACTATITLHSFCPVTDIFLTIAPALSCNS
                     IQSDITFTGVTHCQYTHSTGFIRFGLAPVLSQFGCFLHMATKKFGFYTVTGNAVAKIY
                     PQIKKEIGAIVTNTRITGVRIYIANIDQEFAFHQKKELCKALAVSDDSVSIMQVANVT
                     NHGNAIDVFFSTGDIPSMLSFTMPLYDDNGSFVFEDTMMHEFLHAVINTCLHSYEYIP
                     LPIVEEALPFLLAAGYDATVAQKIQPKIVESEVYTMCKQFRD"
     gene            complement(1280..1666)
                     /locus_tag="PK348_09490"
     CDS             complement(1280..1666)
                     /locus_tag="PK348_09490"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HXK66489.1"
                     /translation="MKVEQRINYGYVIYDITGDITYDNFTEIEDAIKDTLPDNFINVV
                     LNIERVPYINSSALGWLVKLMKEVNAKGYHFFLMNVNQEIMGLLKLTNTLQYFKIIPN
                     EQVLVEKEKKKELDKILNEIEEEKQE"
BASE COUNT          557 a          401 c          336 g          572 t
ORIGIN      
        1 taccgggttg tgttgctatg tagcgctgat acttcagcag catcttcaaa aacgtatgat
       61 attgaagtgt ggatgcctgg attaaaccga tatgtggaga tatcttcatg ttccaatttt
      121 gttgactatc aggcacgccg tgcacgcata cgatatcgca aaaaagaagg tgaaaagcca
      181 gtgtttgtgc atacgctcaa tggctcagga cttgctgcag ggcgtaccct tgcagccgta
      241 atggaaaatt atcaaaccaa ggatggcacc ataaccatcc ctgatgtgtt gaaaccatat
      301 ttagaaaaaa cactataaat gttgcaggta cacacaacaa cctttgcagc tgcgcgacag
      361 gcactcgctt tagcgctagt gaaacaaaaa gaatgttcta taagtattga ccctttggtg
      421 caacagcaca tccactataa ccgcacgctg caggcaatcg ttgaggtatt acatcaactg
      481 aacatttcct gcacatatca taacggtatc atcactgttt tgcctcagaa gctgcctgca
      541 tgcacagcta ctatcacact gcattcattt tgccctgtga ctgatatctt tttaactatc
      601 gcacccgcat tatcatgtaa cagcatacag tcagatataa cgttcacagg cgtcacccat
      661 tgccagtaca cccattcaac aggttttatc cgctttggcc ttgcccctgt gctgtcacag
      721 tttgggtgct ttttgcacat ggccacaaaa aaatttggtt tttatacagt aactggcaat
      781 gcagtagcaa aaatatatcc acagataaaa aaagaaatag gggcgatagt taccaataca
      841 agaatcaccg gcgtacgtat atatattgcc aacattgacc aggaatttgc ctttcatcaa
      901 aaaaaagaac tttgcaaagc actggcagta agtgatgaca gtgtgagtat catgcaggta
      961 gcaaatgtta ccaatcacgg caatgcaatc gatgtgtttt ttagcactgg tgatatacct
     1021 tcaatgctgt catttaccat gccgctgtat gatgataatg gtagttttgt atttgaagac
     1081 acaatgatgc atgaattttt acatgcggtt ataaacacat gtttgcacag ttatgagtac
     1141 ataccgctgc caattgtgga agaagcactg ccgtttttac ttgcagcagg ttatgatgct
     1201 actgtcgcac aaaaaataca accaaagatt gtggaaagtg aagtgtatac gatgtgtaaa
     1261 cagtttagag attaatcccc tattcctgtt tttcttcctc tatttcattc aaaatcttat
     1321 ccaactcttt cttcttttct ttttccacaa gcacctgctc attggggata atcttgaaat
     1381 actgcaatgt attggttagc ttaagaagcc ccatgatctc ctgatttaca ttcatcagga
     1441 aaaagtgata cccttttgca ttaacttctt tcatgagttt aacaagccat cccagtgcag
     1501 agctgttaat atagggaacc cgttcaatat tcagcacaac attaataaaa ttatctggta
     1561 gagtatcttt tattgcatct tcaatttctg taaaattatc gtaggtgatg tctcccgtta
     1621 tgtcataaat tacataaccg taatttatgc gttgttctac cttcataatg ctctgacccc
     1681 aaaataattt atctattatt tcacgcctca agtgctaaga acgctaaaat gtactgtgta
     1741 caaagatttt atagcttaac aacctgtgtt tactctgcgt aaaactattt tactccattt
     1801 gccctgcatg tattacttga gttgctgtcc caaaccaata tcagccacct tatttattct
     1861 ttcaac
//
LOCUS       DAOSZP010000474         1858 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_46843_length_1858_cov_3.057127, whole genome shotgun sequence.
ACCESSION   DAOSZP010000474 DAOSZP010000000
VERSION     DAOSZP010000474.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1858
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46843_length_1858_cov_3.057127"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(179..679)
                     /locus_tag="PK348_09495"
     CDS             complement(179..679)
                     /locus_tag="PK348_09495"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HXK66490.1"
                     /translation="MLLVIALVIVILVLINKWKQIVELVSMQRIPQPVERYYSMLYEK
                     DDAVKKDTDTLLLATSPEYSYTNAWLVEKSGPETGKKFPLIWDEVTIGRDKENAIVIN
                     DDAVSLKHAKIKKIGNTYYLFDLASDNGTFLNNKKLLRPKPLSDWDEIQIGRTSILFR
                     GTKIKQ"
     gene            complement(746..1627)
                     /locus_tag="PK348_09500"
     CDS             complement(746..1627)
                     /locus_tag="PK348_09500"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HXK66491.1"
                     /translation="MKQQLTYAVVVVVTVLLYIPGVLVAGPIVSIERISVDLFPEITI
                     KGVIRNPEGIQISTIGRENFAIVEDNTHINDFTVNSLDTDTYIALCIDASKSISKKNF
                     LRLKKIAINIIKRSKGNYTFIVYKFNDEVVTLAPFSKDTTNYIEIIESLQQHGKRTKL
                     YNCLFAATEELARQKGGKSIILLTDGKDEGSFMFPDDVVNNAREHTIPIFTIVPERCS
                     KAAAVSRLSKITKGKLFVATAGVEEAVINVLQSRMLHTIPFEITFKTNKHDDAIHPIE
                     VQFKHKGIQDHDTGYAK"
BASE COUNT          561 a          350 c          306 g          641 t
ORIGIN      
        1 gttgtaatta ctttagaagt acctttgtaa cgcgcatcaa agatcgtaac agtaagccaa
       61 tcatagtgtg ctaatgcttc tttaatgtat aaaagaccca atggttctga acggtgaaag
      121 ctataataga aatcaaatat tggtggtgat accaacagta ccttgagtga catagtattt
      181 actgctttat cttagttccg cggaaaagga tacttgttcg ccctatctgt atttcatccc
      241 agtctgagag aggtttagga cgcaataatt ttttattgtt taaaaatgtg ccattatcgc
      301 ttgcaaggtc aaaaagatag taggtattcc caattttttt aatttttgca tgtttcaggg
      361 aaacagcatc atcgttgata acaatcgcat tttctttatc tctgcctatc gttacctcat
      421 cccatatgag aggaaatttc ttccctgttt caggtccgct tttctcaacc agccatgcat
      481 ttgtataact gtattcagga gatgttgcaa gaagcagtgt atctgtatct ttttttacag
      541 catcatcttt ttcatagagc atagaatagt atctttcaac gggctgtggt attcgttgca
      601 tgcttacaag ctcaacgatc tgtttccatt tatttattaa taccagaata acaataacca
      661 gcgctatcac caaaagcaca acaatccctg ccagcaccac aaatggcaga tcagtatgta
      721 ttgtatatgt attcttttga aaagattatt ttgcatatcc cgtatcatga tcctgtatgc
      781 ctttgtgttt aaattgtact tctatgggat gaatggcatc atcatgttta tttgttttaa
      841 acgtgatttc aaatggtatg gtatgtagca tgcgtgattg cagtacatta attaccgctt
      901 cttcaactcc cgcagtagca acaaatagtt tccctttggt aattttactt aacctgctta
      961 ctgcagcggc ttttgaacac cgttcaggga caatggtaaa aatgggaatt gtatgttcac
     1021 gggcattatt gacaacatca tcagggaaca taaaactgcc ttcatctttt ccatcggtta
     1081 gcagaataat ggattttccc cccttttggc gagctaattc ttctgtggct gcaaacaaac
     1141 aattataaag cttggttcgt ttgccatgct gctgtaaaga ttcaattatt tctatgtaat
     1201 tggtcgtatc ttttgaaaat ggagccagtg ttacaacttc atcattaaac ttatatacaa
     1261 taaacgtata attgccttta cttcttttta taatgtttat ggctattttt ttaagcctta
     1321 aaaaattttt ctttgatata ctcttgcttg catcaataca cagtgcaata tatgtatctg
     1381 tatccagtga gtttacagta aaatcattga tatgtgtatt atcttcgacg atggcaaaat
     1441 tttccctgcc aatagtacta atctgaatcc cttcagggtt gcgtataaca ccctttatgg
     1501 taatctcagg aaataaatcc acactgatac gttcaatcga cacaattggc cctgcaacca
     1561 gcacacctgg tatataaagc aatactgtga ctacaaccac aactgcatat gttagttgtt
     1621 gtttcataga ttttttcata ataacaaaat gtaataaaaa atttatttta taaactcatt
     1681 actactaatc aaaccaggct tgacacattt atccttctgg aaaacttctt cctaaaatta
     1741 actcacgtga tagtattaaa acagataaac agtaacgata tgtgtactat atatagtatc
     1801 ggtatagatg aatgggcagt acaatcaaat tttactcgtg agcacagtct ttaacctg
//
LOCUS       DAOSZP010000475         1856 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_46896_length_1856_cov_3.571349, whole genome shotgun sequence.
ACCESSION   DAOSZP010000475 DAOSZP010000000
VERSION     DAOSZP010000475.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1856
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46896_length_1856_cov_3.571349"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..532)
                     /locus_tag="PK348_09505"
     CDS             complement(<1..532)
                     /locus_tag="PK348_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003340551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HXK66492.1"
                     /translation="MAYNVLATSQTPALIIYLLDVSGSMSTNLNGKPRIEVVTDALAA
                     TLQQMVFRCTKGGQISPRYRIAMYAYSDSVYDILGGVMTIDQIANLGVPELSTMRSTA
                     TDLAFEEVERLLQQELPSLNDCPAPLVCHMTDGEYTGDDPEPIVRRIMRMSNADGNVL
                     VENIFISDQLLDEPVTD"
     gene            complement(678..1730)
                     /locus_tag="PK348_09510"
     CDS             complement(678..1730)
                     /locus_tag="PK348_09510"
                     /inference="COORDINATES: protein motif:HMM:NF024183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HXK66493.1"
                     /translation="MSNQTGPLKGFRPLKSTDSSTPKPENPPSPTPPIGPNSTSYQPN
                     QGFVRPTIINTTTSSLQKLICPFCSSPIPKGASFCSKCGNPIPKSSPISTQPLDIKCP
                     QCGASNLPGSVYCSSCGAALTKNGQTQGNKLSNLNPHIHLPDAISNLKTDMERFWTYI
                     GGAFAILAFICFFLPFLVLKINNAFSFLISGPDSITISQSGFQFLTASSPSISGVGSL
                     GDTTENLYNELDMGKQIMQYADSSMRTMYIISRILTLIILLLSIANLVVVYQAYAIRE
                     AKWSKWMMAIGVVAPILLIIFYSTAGISFKTGTEELDLLVNSMVKISNGFGFWGMLIG
                     FLGIAFSGYMRSKSHN"
BASE COUNT          566 a          352 c          396 g          542 t
ORIGIN      
        1 tatcagttac gggctcgtcg agcagctggt cagatatgaa aatattttct acgagcacat
       61 tgccatctgc gttagacatg cgcattatgc gtctcacaat tggttctgga tcgtcaccag
      121 tgtattcccc atcggtcata tggcaaacta agggagccgg gcagtcattt aagcttggca
      181 gttcttgctg caacaagcgt tcaacttcct caaatgcgag gtcggttgcg gtagaacgca
      241 tcgtcgataa ctcagggacg cccaggttgg caatttgatc aatggtcata actccgccaa
      301 ggatatcata tacgctatcg ctgtacgcat acatagcaat tcggtaacgt ggtgagattt
      361 ggccaccttt ggtacagcgg aacaccattt gctgcaaagt cgctgcgagt gcatctgtta
      421 ccacttcgat gcgtggtttg ccgttcaagt ttgtagacat cgatccactg acatccagca
      481 aataaataat gagggcaggg gtttgactag tagctaaaac attgtaagcc ataaaaatcc
      541 tcctaaaaca attggtttca taaacaattt atcaaaaagt tttttctcac tttgcccgtt
      601 ctttactaaa caatggaaaa gaattagggc aagacctttc caggcaaatt ttctgcaaaa
      661 accgtagtat cattttatca attatgtgat ttgcttcgca tatatccaga aaaagcaatt
      721 ccgagaaaac caatcaacat tccccaaaat ccaaagccgt tagaaatttt gaccattgaa
      781 tttactaaca ggtcaagctc ttcggttcca gttttgaagc ttatgccagc agtagagtaa
      841 aagataatca ggaggatggg agctacgaca cctattgcca tcatccattt gctccatttg
      901 gcttctctta tagcataagc ttgataaaca acaactaaat ttgcaataga taatagcagt
      961 atgatgagcg ttaatatacg actgatgatg tacatcgtac gcatagaaga atccgcatat
     1021 tgcatgattt gtttacccat atcaagctca ttgtacagat tctcggtcgt atcccctaaa
     1081 ctacccacac ctgaaattga cggtgagcta gcagtcaaga attggaagcc actctgtgaa
     1141 atagttattg aatctgggcc gctgataaga aatgaaaatg cattgttgat tttcaaaaca
     1201 aggaatggta aaaagaagca aataaatgct agaattgcaa atgcaccgcc aatatatgtc
     1261 cagaaacgct ccatgtcagt ttttaaattc gatatcgcat ctggaaggtg aatatgagga
     1321 tttaaattgc ttaatttatt tccttgggtt tgcccatttt tagttaatgc tgctccacaa
     1381 gagctacaat aaactgagcc aggcaaattc gaagcgccac attgtggaca tttaatatcc
     1441 aatggctggg tagatatagg gctggatttt gggatcgggt taccacattt gctgcaaaat
     1501 gatgctcctt ttgggatagg gcttgagcaa aatggacaaa ttaacttctg tagagaggaa
     1561 gtagttgtgt tgataatagt aggtcttacg aatccttgat ttggttgata agaggttgaa
     1621 ttaggaccaa tcggtggagt gggtgaaggt ggattttcgg gtttaggcgt agaggagtca
     1681 gtgcttttaa gtggacgaaa acctttgagc ggaccagttt ggtttgacat tatttcctcc
     1741 tataatattt gtaataaccc tttgtagtgt aattataaaa taaaaaaact aaatctgggg
     1801 cagttttcat atcattaatc tgattcaaga agatcgcatc tataaaatga attggt
//
LOCUS       DAOSZP010000476         1856 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_46897_length_1856_cov_3.561355, whole genome shotgun sequence.
ACCESSION   DAOSZP010000476 DAOSZP010000000
VERSION     DAOSZP010000476.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1856
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46897_length_1856_cov_3.561355"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..343)
                     /locus_tag="PK348_09515"
     CDS             complement(<1..343)
                     /locus_tag="PK348_09515"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HXK66494.1"
                     /translation="MKHINVAFIGLGRIADLHYLGYKNNKHAKLYAVCDINQKLVEQR
                     AKQWHATAAYTDYNDLLKDPHIDAVEILTPHSLHEPVVIAAAKAGKHIALQKPMTVDL
                     ASADRILAATRE"
     gene            746..1231
                     /locus_tag="PK348_09520"
     CDS             746..1231
                     /locus_tag="PK348_09520"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HXK66495.1"
                     /translation="MVTEGKYVAIHYTGKFDDGQVFDSSLDGAPFEFQAGAGMVIPGL
                     DRAVLGMKLDEEKDIVIAPEDGYGDYDENLLYAFPLEEVQQQFKPEIGMTIGLQMDNG
                     AQIPAVIKEITDDEVVVDMNHPLAGKTLHFHVKVVNISDEPEYSGGCDTCGGYSDGCS
                     C"
BASE COUNT          570 a          356 c          370 g          560 t
ORIGIN      
        1 actctcttgt tgctgcaagt atacggtcag cacttgcaag atcaacagtc attggctttt
       61 gcagggcaat gtgctttcct gcttttgctg ccgcaataac cacaggctca tgaagtgagt
      121 gcggcgttaa gatttcaaca gcatcaatgt gaggatcttt tagcaggtca ttataatcag
      181 tgtatgcagc agttgcatgc cattgtttag ctctttgctc aaccagcttc tgattgatat
      241 cacatacagc atacagtttt gcatgtttat tatttttata tccaaggtaa tgaaggtcag
      301 caatacgccc caatccaatg aatgcaacat taatgtgttt catgacaccc tccagtcatt
      361 gcacattata aaatgctatt gcatatatgt caattcattt ttgctgtcat gtccaatatc
      421 gtttcgtgct acaatagcta caatcctcaa tcattccagg tttgatactg aatccacttg
      481 catactgaca ttctgattca tgtccacgat aattctattc ccggtaacta tcgcccacca
      541 attatctaat tttttcttaa aaaaatattg atattgacac attcatagag tatcttattg
      601 tggtagtata taattgctgt gttaactata tttttggtag catgtaatgc tgtaatcacc
      661 tgcccacagt aaccaaccca caggattatt gcattaacac acaaaaattg cagcaattac
      721 caattaaaat atgtaaggag tttttatggt aacagaaggt aaatatgtcg ccattcatta
      781 caccggaaag tttgatgacg gtcaggtgtt tgattcaagt ttagacggtg caccttttga
      841 atttcaggcc ggagctggca tggttattcc aggtctggac agagcagtgt tgggcatgaa
      901 gcttgacgaa gaaaaagata tcgttattgc ccccgaagat ggttacgggg attatgacga
      961 aaatctgtta tatgcatttc cacttgaaga agtacagcag cagtttaagc ccgaaattgg
     1021 gatgactata ggtttgcaga tggacaacgg tgcacagatc cctgcagtta tcaaagagat
     1081 aaccgatgat gaggtggttg ttgatatgaa tcatccactt gcaggaaaaa cgcttcattt
     1141 ccatgtcaaa gtggtcaaca tcagcgatga acccgagtac agcggtggat gtgatacatg
     1201 cggtggttat agcgacggct gcagctgtta agtacctata ccgtgcaaaa acacacagcc
     1261 acgtatctat aacaacgcac tggtagcaaa taaacacttt tccactcaat acaacgtaaa
     1321 aaagaggccg tcccaaaaca atcatggggc ggcttttttt attttaagta tttgatagtg
     1381 gaaatattta actctactaa ctccacaatc tccttgttgt caaagtatac ataattttac
     1441 tcgttgccgt atccggaata tgttatgatt ctattcggtg cttttttaaa atgttttttc
     1501 agattatcac tatgtggctt aaggcaggtg gtgtctgctg ggttacagca ttactgcagt
     1561 aaaagccctg tgcaaaaaga atcctctgca ctgcctcacg ccgcacccgt ttaaaatgaa
     1621 gggagcggcg atgaggcatt aaataatagt tgacaaatat atatttatat atttatatac
     1681 tcaaaactct ataaaggcag tgtatatgga aagggcaata ataagtgtta aaaattatga
     1741 atcacaacgc ataataaaag aaagccgcaa gaaagccatt gatgaaggat tcactttcag
     1801 tgaggctgtt attaggctgc ttgaaaaatg gatcaacaat gaagttcgca ttgaaa
//
LOCUS       DAOSZP010000477         1848 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_47163_length_1848_cov_2.813720, whole genome shotgun sequence.
ACCESSION   DAOSZP010000477 DAOSZP010000000
VERSION     DAOSZP010000477.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1848
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47163_length_1848_cov_2.813720"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="PK348_09525"
     CDS             complement(<1..210)
                     /locus_tag="PK348_09525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66496.1"
                     /translation="MLATYVIPLDSTTGVKTNRQREKKLFATTDFFDNIVINHRGSRY
                     GNPETRTFEYYHKHKCFMDKAQLDQL"
     gene            complement(335..>1848)
                     /locus_tag="PK348_09530"
     CDS             complement(335..>1848)
                     /locus_tag="PK348_09530"
                     /inference="COORDINATES: protein motif:HMM:NF012516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HXK66497.1"
                     /translation="ILLLSVFSSEAKDYLITDFGAVPNSNVLNTKAIQAAIDKANDEG
                     GGMVKVPVGTFLSGTIYLKSNVILHLSPGSVLLGTPKKELYENEALIYAEDQHDIAIS
                     GNGKIDGQGWHSNFRSKNHLNGIPGRPIAVWFKNCRFVTLENFTLVNSACWCIKLQEC
                     VFATADKLNVLSRVVANNDGIDVCDCHNVIISNCKFDCGDDAICVKSDSYFGVENMTV
                     SNCIIRSESNGIKFGTSSVGGFRNVTINNCVIYDTRLSGIALEVVDGATMQNISIGNI
                     TMKEVNGGIFIKLGHRSGEKPGILRDVIIHDIIATGIGIWKPDTTASYFKLPKGSPLI
                     GMSVLGLPGYEVENILLSNIYMQFAGGGSSEDACREIEDTPSVYPEYTNAGITPAYGI
                     NVKNAKRISLSNVRLEYIEEDFRPAFYLKNVEEAELSQVKMKISPEAGSFIMLEDVRN
                     VVISQCKPAAKTVPFLELKGIIENISVINNDLSQVKQPFVSNRTVSQSEIKIK"
BASE COUNT          532 a          406 c          316 g          594 t
ORIGIN      
        1 cagttgatcc aattgagcct tatccatgaa gcatttgtgt ttatggtaat actcaaacgt
       61 cctggtctct gggttaccgt acctgcttcc cctgtgattg ataacaatgt tatcgaaaaa
      121 atccgtcgtt gcaaaaagtt ttttttcgcg ttgtcggttt gtttttactc ctgttgtact
      181 atccaaagga atgacataag tagccaacat tattctcccc atccccataa gagatgttaa
      241 tataaaggct ctgcgcgaaa tgttcttatt catctctaaa ataaaatatt cgtacaattc
      301 attatacatg gatctgcaaa aaatccttta aactttattt aatttttatt tccgattggc
      361 taacagttct atttgacacg aaaggttgtt taacctgact caaatcgtta ttaattaccg
      421 atatattttc aatgattcct ttcagttcaa gaaatggaac tgttttggcg gcaggtttac
      481 actgggagat gacaacattt cgtacatctt caagcatgat gaaggaacca gcctctggcg
      541 aaatcttcat ttttacctga ctcaattcag cctcttcgac attttttaaa taaaatgcag
      601 gcctgaagtc ttcttctatg tattcaagtc ggacgttgct taagcttatt ctcttcgcat
      661 tttttacatt gattccgtaa gcaggtgtta ttcctgcatt tgtgtattcg gggtaaactg
      721 agggtgtatc ttctatttca cggcaggcat cttcagacga tccgccgcca gcaaattgca
      781 tataaatatt acttaacaaa atgttctcaa cttcatagcc aggcagtccc aaaacagaca
      841 ttccgataag aggagaacct ttaggtaatt tgaaatagga agctgttgtg tctggcttcc
      901 aaatccctat tcctgtagct ataatatcgt ggataatcac atccctcaat atgccgggtt
      961 tttccccact acgatgcccc agctttatga atataccacc gttgacctcc ttcatggtga
     1021 tgtttccgat actaatattt tgcatggttg caccatctac aacttcgagt gcaatacctg
     1081 acaggcgcgt atcgtaaatc acacaattgt taatcgtaac attacgaaat ccacctacac
     1141 tgctggttcc aaattttatc ccgttcgatt cacttcttat aatacagttc gaaactgtca
     1201 tgttttctac accaaaataa gagtcgcttt tgacacatat cgcatcgtcg ccgcaatcga
     1261 atttacaatt tgaaatgata acattatgac aatcacaaac gtcgattcca tcgttgttgg
     1321 ctactacacg actgagcaca ttcagcttat cagcagtagc aaaaacacat tcctgaagtt
     1381 taatacacca gcaggcactg tttaccaatg taaaattttc gagagtaaca aatctgcagt
     1441 ttttaaacca aacagcaatc ggtctgcctg gaatcccatt taaatgattc ttggaacgaa
     1501 aatttgaatg ccatccctgt ccgtctattt taccatttcc ggaaatagca atatcgtgtt
     1561 gatcttcggc atatatcaac gcctcgtttt cgtatagttc ctttttcggc gtgcccagca
     1621 aaacagaacc tggggaaaga tgaaggatga cattggattt taagtagatg gttcctgaaa
     1681 gaaatgttcc gacaggcact ttaaccatac ctcctccctc gtcattggct ttatctatcg
     1741 cagcctgaat tgcttttgtg tttaaaacat tactgttagg gacagcacca aaatctgtaa
     1801 tcaggtaatc tttcgcctca ctgctaaata ctgacagcaa aagaatga
//
LOCUS       DAOSZP010000478         1843 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_47333_length_1843_cov_3.062081, whole genome shotgun sequence.
ACCESSION   DAOSZP010000478 DAOSZP010000000
VERSION     DAOSZP010000478.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1843
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47333_length_1843_cov_3.062081"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1843
                     /locus_tag="PK348_09535"
     CDS             <1..>1843
                     /locus_tag="PK348_09535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66498.1"
                     /translation="LHGKVYVVLNNTFISLHKYINNTVLYTMGCVPFASRVDGIERLV
                     WLPADVVTTDTKLSSNIHTTANINNSEYIVDTDNPDITITPPYCFANKLECSAVFKSI
                     NNMKTDELMLLADASFYSHIASVILYSGDNVETLLTAVINNDIKLINSLKTKTVVLGK
                     IPDTIKAREQNAQIIVQEKHTEYAKALERGDYDSALSALERWKRAGLQQQEYYDKKIL
                     VLVYHNNIDEALKVMQSHSSAFTSSFSVYTYLLKGDVTSAEKQLNNPHLAGTFDYTVY
                     ESIIKGYKHKEKLTVINNVSKSTASILPANQLLLLYAEEAFLLGEKEKAKHIMELYQA
                     DYIPSQRELLVAYNLGYSIASHSNTVAYRMLKAITGNSNSSNFINTINEYNNLLLFGI
                     LEIVKNRPLMPDEEWQRIMLIAEKGKHMGIDVQFTTAKIAETLQGIIPARVIQYIKTV
                     AVDKGKIVSPIVNLYAINAYQKTDNFNDAYTACKTLKQLPYTLEQLWYTYYIYSAIML
                     GKTDDAQELLNNSLIATWDLVMYNVFNVCIKLQKVVALKTTDSNMLQTIAQNIDVLIT
                     SLHDESIRWYYSEYKYLLDKLINYAISYAMSRGNQRDALKYAELHKM"
BASE COUNT          636 a          309 c          349 g          549 t
ORIGIN      
        1 cgttacatgg gaaagtctat gttgtgctca ataatacatt catttcactg cataaatata
       61 taaacaatac agtgttatat acaatggggt gtgtgccgtt tgcttcacga gtggatggta
      121 ttgaacgcct ggtctggttg ccagcagacg tagttactac agatacaaag ctttccagta
      181 atatacatac cactgctaat attaacaatt cagagtatat agttgatact gacaatccag
      241 atattactat aacaccgcct tactgctttg ctaacaaatt ggaatgctct gctgttttta
      301 aatctataaa caacatgaaa actgatgaac tcatgctttt agccgatgca agcttttatt
      361 ctcacattgc atctgttatt ctgtattctg gagacaacgt tgagactttg cttacagcag
      421 tgataaataa tgatataaaa cttattaatt ctctgaaaac aaaaacggtg gtgcttggaa
      481 aaattccaga cacaataaaa gctagggaac aaaatgcaca gataattgtt caggaaaaac
      541 atactgagta tgccaaagct ttagaacgtg gcgattatga ttcggccttg tctgctttag
      601 agcgctggaa aagggcagga ttacaacaac aagaatatta tgacaaaaaa atacttgttc
      661 tggtttacca taataacata gatgaagcac tcaaagttat gcagtctcac agtagtgctt
      721 ttaccagttc attttctgtt tatacatatt tgttaaaggg tgatgttaca agcgcggaaa
      781 agcaactgaa caatcctcat ctggcaggta catttgatta taccgtttat gagtctatca
      841 ttaaaggcta taaacataaa gaaaagctta ctgttattaa caacgtatca aaaagtactg
      901 cttcaatttt gcctgctaac caattgttgt tgctgtatgc cgaggaggct tttttgctgg
      961 gagaaaagga aaaggccaaa catataatgg aattgtatca ggcggactat attccttctc
     1021 aacgggagtt gctggttgca tacaatttgg gttattcaat agcttcgcat agtaatacag
     1081 ttgcatacag gatgctaaaa gctattactg gtaacagtaa tagcagcaat tttataaata
     1141 ccataaacga atataataac ctgttgcttt ttggaatact ggaaattgtc aaaaatagac
     1201 cactaatgcc cgatgaagaa tggcaacgta tcatgctgat tgctgaaaag ggcaaacaca
     1261 tggggattga tgtacaattt acaactgcaa aaatagctga aacattgcag ggtatcattc
     1321 cagcaagagt aattcagtac attaaaacag ttgctgtaga caagggaaag attgtatcac
     1381 ctatagtaaa cctgtatgca atcaatgcct atcaaaaaac agataatttt aatgatgcat
     1441 atactgcatg caaaacctta aagcaattgc catatacact ggagcagtta tggtacacct
     1501 attatatcta ttcagcaata atgttaggga aaactgatga tgcacaggag cttttaaaca
     1561 atagtctaat agctacctgg gatcttgtta tgtataatgt tttcaatgtt tgcatcaaac
     1621 tgcaaaaggt tgtggcatta aaaaccactg atagcaatat gttgcaaact atcgcccaaa
     1681 acattgatgt gttgattacc agcctgcatg atgaatcaat acgatggtat tattctgagt
     1741 ataaatactt attggataaa cttattaatt atgcaatatc gtatgcaatg agcaggggaa
     1801 atcagcggga tgctctgaag tatgctgaat tacataagat gat
//
LOCUS       DAOSZP010000479         1843 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_47345_length_1843_cov_2.213087, whole genome shotgun sequence.
ACCESSION   DAOSZP010000479 DAOSZP010000000
VERSION     DAOSZP010000479.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1843
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47345_length_1843_cov_2.213087"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            92..1516
                     /locus_tag="PK348_09540"
     CDS             92..1516
                     /locus_tag="PK348_09540"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HXK66499.1"
                     /translation="MYKIAIDDGHGMQTAGKRTPVFTDGTTGETGKPWMHENEFNRAV
                     AAYLKEHLIRCGFDVLMVAPTDEDTPRMTRINRANDWGADLYISIHANALNGIWGPQE
                     GVSTFHFPGSVKGQKAAEIIHKYLLQGTKQKDRKVQTAKFDVLRYTYMPAVLVECAFM
                     DNLNEARLLMSDAFRRECAYDLARGICEYFNIQYVETKTYKKVSITGKTQYSTVKRGS
                     KGLDVVALQEQLKDMGYYTGAIDGIFGQRTEDAVRYVQKANDLTIDGIVGPLTWANIL
                     TAHVYEVSPLSLRNEIVNQPGNKIKGDFINSVFFEPSTLRSLGNMVQDGKLLAKQWVT
                     HPDTGKPHDVVKRGNLIVYNDGTVVVKMIMDIEKEEDISKIKFAVSGFNMEPLDLKKE
                     WFDPAQVGYTTWRSALGYNPSTKMINAVIMPNCSAERMRKMMDKLGCTLKLGMDSGGS
                     TNGRFAGKDIRLTTRTLCGIIRFG"
BASE COUNT          616 a          315 c          452 g          460 t
ORIGIN      
        1 aaacttgcgt tatctggctg ataaacagcg tagtagtatg gtttacggct atgaaagcat
       61 atgaagaaat aactaataag ggatgatggt aatgtataaa attgctattg acgatggcca
      121 tggaatgcag acagctggca aacgtacgcc tgtgtttacg gatggaacaa caggagaaac
      181 cggcaaaccg tggatgcatg aaaatgagtt taatagagct gtagctgcct acctcaaaga
      241 gcacctcatc cgctgtggtt ttgatgtatt gatggtagct cctacagatg aggatacacc
      301 gcgcatgacg cgcataaacc gtgccaatga ttggggagct gacttgtata tatcaattca
      361 tgccaatgct ttgaatggta tctggggtcc acaagaaggt gtaagtacat tccatttccc
      421 aggttccgta aaaggtcaaa aagcagcaga aattatacat aaatatctgt tgcaaggaac
      481 aaagcaaaaa gataggaaag tgcaaacagc caagtttgat gttttgcgat atacttacat
      541 gcctgccgtg cttgttgaat gcgctttcat ggataatctt aatgaagcaa gactgcttat
      601 gtcagatgca tttcgccgtg aatgtgctta tgatttagcg cgcggtatat gcgaatattt
      661 caacattcaa tatgtggaga ctaaaacgta caaaaaagta tcaataacag gcaaaacaca
      721 atacagcaca gtcaagcgcg gcagcaaagg actagatgtt gttgccttgc aggagcaact
      781 caaggatatg ggttattata caggtgcaat tgatggcata ttcgggcaga ggaccgagga
      841 cgctgtcaga tatgtgcaaa aagcaaatga cctaaccatt gatggtatag tcgggccact
      901 cacctgggca aacatactca ctgcccatgt ttatgaggtc agcccgttga gcctgagaaa
      961 tgaaatcgtt aatcaacctg gcaacaaaat taaaggagat tttattaaca gtgtcttttt
     1021 tgagccgtcc actctgcgaa gtttgggcaa tatggtgcag gatgggaagc tgctggcaaa
     1081 gcaatgggta actcatcctg ataccggcaa gccgcatgat gttgtaaagc gtggaaatct
     1141 aatcgtatac aatgacggta ctgtggtcgt aaaaatgatt atggatattg agaaggaaga
     1201 ggacatttca aagataaagt ttgctgtgtc aggctttaat atggagccgc ttgacttaaa
     1261 aaaagaatgg tttgacccgg cacaggtcgg ctataccaca tggcgaagcg cattagggta
     1321 taatccgtca actaaaatga taaatgccgt aatcatgccg aactgttcag ctgagagaat
     1381 gagaaaaatg atggataagc taggatgtac attaaagctt ggcatggaca gcggaggaag
     1441 cacaaatgga cgatttgcag gaaaggatat caggctgaca acaagaacgc tctgcggaat
     1501 aataagattt ggataaaatg acccctcgaa agaggggttc tttttttgtg tccattttca
     1561 tataggactt tagacctatt ttaaaaaata ttgtaccaaa agtattgaca tttggtacaa
     1621 tataagatat aataaaaaca aataaaaaac aggaggtaag aaagatgtca aaaataatag
     1681 gctatgaaaa tgccacgatt ggcacggtgt atgtgactgc gaagagaaaa tatcacgtat
     1741 atgaggaaga ggggaaagtg catgttgatg cgatcctgaa tgaaggggaa tccgcacaaa
     1801 cactgggttt tgtgccagag tgcgacgaca aatactattt tgg
//
LOCUS       DAOSZP010000480         1832 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_47691_length_1832_cov_3.917276, whole genome shotgun sequence.
ACCESSION   DAOSZP010000480 DAOSZP010000000
VERSION     DAOSZP010000480.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1832
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47691_length_1832_cov_3.917276"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..1037
                     /locus_tag="PK348_09545"
                     /pseudo
     CDS             3..1037
                     /locus_tag="PK348_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009155865.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
     gene            1109..>1832
                     /locus_tag="PK348_09550"
     CDS             1109..>1832
                     /locus_tag="PK348_09550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66500.1"
                     /translation="MALTEYTIPSTKINDFFISVSDGMELYCIDFIPKKDSSSKPIIF
                     FVAGWISHITGWKDVLLTLTDEYRVIYIETREKTSSRIPEGKTYKDFSFNVMRMVLDL
                     HEVIQKVIPKNRTFVMAGSSLGSSVILEYLAMHTRKPECALLIGPLPEFRFPPLLGDI
                     VPRLPASWYNVLKELVKWYLRNFRLDTEKEKEQMAKYTNTINSADPYKLKPNALALKN
                     YNLWDKLPFITTPCYIFGAKTDK"
BASE COUNT          600 a          360 c          339 g          533 t
ORIGIN      
        1 aaatcgcttt gtaccctgga tatggtattg gtccctaggt tgtatcagaa gcagcaaaag
       61 ttattgatgc ctgcaacatt gatgttcatt acacaacatt taactggaat acaaccctgt
      121 ataataaaac aggaaaatgt gcacctgaaa attatcttga attactttca tcgtttgatg
      181 caatattatt aggcgcactt ggcaatacac ataatgcccc tgaccatatt gctgttgaac
      241 cgcttctaac tatacgaaaa gcttttgatc aatatgtcaa tatacggcca gcaatgctat
      301 ttcctggtgt tgaatcacct ttaaaaaaca aaaaaccata tgacatagac atgatagtaa
      361 tcagagaaaa cactgaaggt gaatatacag cactgggagg aagacattat attggtactc
      421 ccgacgaagt tgcaatgcaa attaactatt tttcgcgaaa aggaactgaa cgcataatcc
      481 ggtatgcatt tgaaactgcc cgaaaccgca aacgtaaaac tctgacaagc ataaccaaat
      541 ccaatgcact caaatacagc atggtattct gggatactgt tttttacgaa gttgccaaag
      601 agtatcctga tgtaatagca tcatcaatgc ttgttgatgc tgcagccatg aatatggtac
      661 gctgccccga gcaatttgat gttgtagtcg catccaatct ttttggcgat atacttaccg
      721 acctggcagc gatagttgtt gggggaatgg gatttgctgc aagtggcaat atcaatccaa
      781 cacgaaaata cccatcaatg tttgaacccg tccatggttc ggcaccggat attgcaggta
      841 aaggtgtagc gaatccagtt gctgcaatac tcagtggtgc actcatgtta gaatttttag
      901 gtgagcacga tgcagcacaa aagattaaat ctgctgtggt caatcactta aatgatggca
      961 ctgttaagac acccgatcgt ggtggtaatg caacaactgc tgacgttggc acagatattg
     1021 cacagcgtat acaatgaaat gcttgatttt tgtttttgta tatgatattc tcttatagat
     1081 tgcaataata cttaattaga gggttaccat ggctttgacg gaatacacaa taccatcaac
     1141 aaaaatcaat gattttttca tatctgtttc tgatggaatg gaattatatt gtatagattt
     1201 tattcccaaa aaggattctt catccaaacc aattatattc tttgttgcag gctggatatc
     1261 tcatatcacc ggttggaaag atgtcctgct aactttaacc gatgaatacc gtgttatata
     1321 tatcgaaaca cgcgaaaaaa catcttccag aatacctgaa gggaaaacgt ataaagactt
     1381 ttcgtttaat gtcatgcgaa tggtacttga tctccatgaa gttattcaaa aagttattcc
     1441 taaaaacaga acatttgtta tggcaggaag ttcactgggt tcctcagtta tactagaata
     1501 tttagctatg catacccgca agccagaatg tgcattactt attggcccac tccctgaatt
     1561 cagatttcct cctctgcttg gtgacattgt accaaggctt cccgcttcat ggtataatgt
     1621 actcaaagaa cttgtaaaat ggtatttacg caattttcgc ctggatacag aaaaagaaaa
     1681 ggaacagatg gcaaaatata ccaatacaat aaattcagca gatccttata aattaaaacc
     1741 taatgcgctt gcactcaaaa attataatct atgggacaaa ctccccttta taacaacacc
     1801 ctgctacatc tttggagcaa aaactgataa ac
//
LOCUS       DAOSZP010000481         1829 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_47805_length_1829_cov_3.316798, whole genome shotgun sequence.
ACCESSION   DAOSZP010000481 DAOSZP010000000
VERSION     DAOSZP010000481.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1829
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47805_length_1829_cov_3.316798"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(174..944)
                     /locus_tag="PK348_09555"
     CDS             complement(174..944)
                     /locus_tag="PK348_09555"
                     /inference="COORDINATES: protein motif:HMM:NF028441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HXK66501.1"
                     /translation="MKRFLVFSLLPVFCVAIVIVYAMTGREIMEKSDALPEPRTAISK
                     ATMVIYKGGDTLTRTIEMMAMKVGQNDKVLAVVKEEPGGDITKVLTHTNKTGEDMQWV
                     KMSNGKVKRISSGDRSGAFVNSHIFYEDLRSRNIDDYEYTLMGEETVEGFPCYKVEAK
                     PKPGKSIYDKAIFFIIKSGEFQYFAVRIDIFYEGYLYKRLINYNLKKVDGIITPYKAV
                     MYRLDKKGANLGRTEVTITNLQYNNPAINENMFNVGKL"
     gene            complement(1014..>1829)
                     /locus_tag="PK348_09560"
     CDS             complement(1014..>1829)
                     /locus_tag="PK348_09560"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HXK66502.1"
                     /translation="SYIDLYGGKDDNDNGLADEFEPYVDRKFTTVNIFARIWEKEGKL
                     LTSSIMHHTINKIGTYLDTHLPDGYTYKITGEPKVLVRMSEYIVRGQIMSLALSLISI
                     FVVVFMLFNNIRAALVALIPISTAVLLNFGVMGFLGIRLDIATAIIASITIGIGIDDT
                     IHFLNTYRHFDKMNIPQDEVILKTLHQSGKAIVYTSIALIFGFLVLVISSFKPVIYFA
                     VLLAVTMITTTIGALLFLPATIYFFKLNLSPKISTSKVGQILNIGKYFELESD"
BASE COUNT          555 a          361 c          295 g          618 t
ORIGIN      
        1 cgagtattac gatataatgt ttgcaaaatt tactcaacga cctcttttgg gacagtccct
       61 gtaacaatta agctatgatt atgattatct ttgcgctttg agctctctgc tatcaaacat
      121 acatacgtgc tcgcgcaaaa aaacgctgaa taaaaaagta gtgcattatt acatcacaat
      181 ttccccacat taaacatatt ctcattaatt gctggattat tgtattgcag atttgtgatg
      241 gttacttcgg ttctgcctaa gttagcccct tttttgtcca gtcgatacat aacagcttta
      301 tacggtgtaa tgataccatc aacctttttc agattataat taatcaaccg cttgtataga
      361 tatccttcat agaaaatatc tatacgtaca gcaaaatact gaaattcacc tgatttgata
      421 atgaaaaata ttgccttatc gtagatactt ttgccaggtt ttggttttgc ttctacctta
      481 taacagggaa aaccttcaac agtttcttct cccatcagtg tatattcgta gtcatcaata
      541 tttcgtgatc gcaaatcttc atagaagata tgtgaattga caaatgcacc actgcggtca
      601 ccggatgata tgcgctttac ttttccgtta gacatcttaa cccattgcat atcttcgcct
      661 gttttattgg tatgtgtcaa taccttggta atatcgccac cgggctcttc tttaactact
      721 gcaagtactt tgtcattctg cccaaccttc atagccatca tctcgatggt gcgtgttaag
      781 gtatcaccac ctttgtaaat taccattgtt gctttactta ttgcagttct gggttcaggc
      841 agagcatctg acttttccat tatttccctg cctgtcattg catacacaat aactatcgcc
      901 acacaaaaca caggtaacaa actaaatacc agaaaccttt tcattgtatc cctcctatta
      961 aaatggaatt tataattaat atatttttcc cccctttggg gagtttgaat gcatcaatct
     1021 gattccaatt caaagtattt accaatattg agaatttgcc ccacctttga tgtagatatt
     1081 tttggtgata gatttaattt aaagaaataa atggttgcag gtaaaaataa aagtgcacca
     1141 atggttgtcg ttatcatagt aacagccagc aacacagcaa aatatataac tggcttaaag
     1201 cttgatatta caagaacaag aaaaccaaat ataagtgcta tggatgtata tactattgct
     1261 ttacctgact gatgcagggt tttaagaatt acttcatcct gtgggatatt cattttgtca
     1321 aaatgccgat acgtgtttaa aaagtgaatg gtatcgtcaa taccaattcc aatggttatg
     1381 gatgcaatga ttgctgttgc aatgtcaagc cgtatgccta aaaatcccat aacaccaaaa
     1441 tttaaaagta ctgcagtact tattggaata agtgcaacta gtgctgcccg tatattattg
     1501 aagagcatga atactacaac gaatatagaa atcaaactga gagctaagct cataatctgt
     1561 ccacgcacaa tatattctga catacgcacc agtacttttg gttcgcctgt tattttatat
     1621 gtatatccat cgggaagatg agtgtccaga taggtgccaa tcttgtttat ggtatgatgc
     1681 attatacttg aggttaataa ttttccttct ttttcccata ttcgagcaaa tatgtttact
     1741 gttgtaaatt ttctgtcaac gtagggttca aattcatcag ccaacccatt atcgttgtca
     1801 tcttttcctc cataaaggtc aatgtagct
//
LOCUS       DAOSZP010000482         1819 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_48183_length_1819_cov_2.497732, whole genome shotgun sequence.
ACCESSION   DAOSZP010000482 DAOSZP010000000
VERSION     DAOSZP010000482.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1819
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48183_length_1819_cov_2.497732"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..436)
                     /locus_tag="PK348_09565"
     CDS             complement(<1..436)
                     /locus_tag="PK348_09565"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXK66503.1"
                     /translation="MKGQNFLTTFGPMYYRTKDGDANIHLVCIHGAGGDSRLFIPLMN
                     EIKNITVHAVDLPAHGKSKIQECSLDIYVQATMQFIQSLQGNVFVLGHSMGGGIIFEL
                     VKHDAPVKGALFLATAGTLPVNPVVFELLDKDFDSLCRLAVDL"
     gene            576..1160
                     /locus_tag="PK348_09570"
     CDS             576..1160
                     /locus_tag="PK348_09570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66504.1"
                     /translation="MYTSCNYACQPGFNASCALCCGSHNYTASFHDIDALFKDRFKFF
                     SNMLAKSNDIVSALKACYQELSHITLPAIHPDATQCPFVAYTDSSHTIIGCLLYPYCT
                     NFDYRSLFMGAICKTFSCSVNTVHYADRIQTAAKRCGDWYYYSLIIHDMDLLDLLCNS
                     PAEHTIEECKQKAATMLQNQKACSTGIITKDTAK"
     gene            1299..1772
                     /locus_tag="PK348_09575"
     CDS             1299..1772
                     /locus_tag="PK348_09575"
                     /inference="COORDINATES: protein motif:HMM:NF013340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HXK66505.1"
                     /translation="MNISSRSFTNGGTIPAKYAMKAIAGGMNVSPHIAIGDIPKEARS
                     LAIAFVDRHPMARNWVHWLVINIPAHTTEIPEGASLKSMPPESIEMINTFGFAGYGGP
                     QPPRGSGVHVYELTCFALSESFKMPKKELTESEFLKLIKPLLITQCKMTGNFENK"
BASE COUNT          583 a          391 c          318 g          527 t
ORIGIN      
        1 tcaggtcaac tgccagacgg cataaactat caaaatcctt atccaacagt tcaaatacaa
       61 cagggtttac tggcagcgtg ccagctgttg ctaaaaacag cgcacctttt acgggtgcat
      121 catgtttcac cagctcaaaa attatgccac cacccattga atggccaagc acaaagacat
      181 tgccctgtaa agactgtata aattgcatcg ttgcctgcac atatatatcc aatgagcact
      241 cctgaatttt agattttcca tgagcaggaa gatctacagc atgtaccgtt atatttttta
      301 tttcattcat taatggaata aataagcgtg aatctcctcc tgcaccatgt atacatacaa
      361 gatgaatatt agcatcacca tcttttgtgc gatagtacat gggcccaaat gtagttaaaa
      421 aattctggcc tttcattatg tatttcccct gcaattaaat tatatacaat tcatcttttt
      481 ggctatagta taaaacaata catgtataca cgcaaattgt cgagctataa tttgcttaat
      541 acggcatact actataataa acgaataatt gatttatgta tacctcatgt aattatgcgt
      601 gtcaaccagg ttttaatgct tcgtgtgcgt tgtgctgtgg cagccacaac tatacggcat
      661 catttcatga cattgacgct ttatttaaag accggtttaa atttttcagc aatatgcttg
      721 ctaaaagtaa tgatatcgta tctgcactga aagcctgtta tcaggaacta tcgcacataa
      781 ctcttcccgc tatccaccct gatgcaacac aatgcccttt tgtagcatat accgacagct
      841 cacatacaat tattggttgt ctgctttacc cttactgtac caattttgat tatcgaagtc
      901 ttttcatggg agctatctgc aaaacattta gctgcagtgt aaacacagta cactatgctg
      961 acagaataca gacagcagca aaacgttgtg gcgactggta ctactatagt ctgatcatcc
     1021 atgacatgga tcttttagac ctgctatgta actcaccagc agaacacacc attgaagaat
     1081 gtaaacaaaa ggcagcaaca atgctgcaaa atcagaaagc atgttcaaca ggtatcatta
     1141 ccaaagatac tgcaaagtaa cggtctctta ctcaagaaag atagtaaact ggtttgtttc
     1201 agttacaatg gaaacaaaaa attgtattgc ttttcaccta attcatgatg taaaatttta
     1261 ttcagataca gatactaaaa cagaaaaagg aggaagccat gaatatctca tcccgctcgt
     1321 ttaccaatgg tggaactatt cctgcaaaat atgctatgaa agccatagca ggtggtatga
     1381 atgtatcacc acacattgcc attggcgata tacccaaaga agcccgttcc cttgcaatag
     1441 cctttgttga tcgacacccc atggcacgca actgggtgca ctggcttgtt atcaacattc
     1501 ctgcacatac cactgaaatc cctgaaggtg catcacttaa atcaatgccg ccagaatcta
     1561 ttgaaatgat aaataccttt ggttttgccg ggtacggggg gccacaacct ccacgcggaa
     1621 gtggtgtgca cgtctatgag ttgacatgct ttgcactctc cgaatcattc aaaatgccaa
     1681 agaaagagct cactgaaagt gaatttttaa agcttataaa gcccttgctc attacccagt
     1741 gtaaaatgac gggtaatttt gaaaataaat aggctgaata gtatacaata aaacagttga
     1801 ctattattgt gttttccac
//
LOCUS       DAOSZP010000483         1816 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_48280_length_1816_cov_3.030664, whole genome shotgun sequence.
ACCESSION   DAOSZP010000483 DAOSZP010000000
VERSION     DAOSZP010000483.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1816
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48280_length_1816_cov_3.030664"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..799
                     /locus_tag="PK348_09580"
     CDS             <1..799
                     /locus_tag="PK348_09580"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004788643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HXK66506.1"
                     /translation="AISTKDESKERLQELVKAGVNVVVIDAAQGNSIYQIEMIKYIKK
                     KYPELDVIGGNVVTIEQCENLIKAGADALRIGMGPGSICTTQVTMAVGRAQSTAVYRC
                     SKFAKQYNIPTIADGGIATIGHIAKALALGASTAMMGSMFAGTEEAPGEYFYENGVRL
                     KRYRGMASLEAMEKGGSKRYFAEDSKILVAQGVSGAVVDKGSMFDYVPYLVQGLKHAF
                     QDMGVRTIKELHEKLYKEELRFELRSLSAQIEGGVHTIYSYKEPKYL"
     gene            complement(803..1462)
                     /locus_tag="PK348_09585"
     CDS             complement(803..1462)
                     /locus_tag="PK348_09585"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HXK66507.1"
                     /translation="MGIDGVIFDLDGTLLDTIADLAYCVNNVLLRYGYRTHPVEAYKQ
                     FIGDGMAMLIQRAVGETCSDKEITKLVSELKDEYAKNWNRETKPYPGVHDLLKELSHK
                     KIKISVFSNKSHEFTVEMVKYYFPDIAFSTILGLQDSIPRKPDPHGALLIAHTMQLEP
                     TQIAMIGDSATDVEMAHACGMYSIGVSWGYRPVALLLQHHPHAIANTPGDIIRIITSV
                     T"
     gene            complement(1468..>1816)
                     /locus_tag="PK348_09590"
     CDS             complement(1468..>1816)
                     /locus_tag="PK348_09590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="short-chain dehydrogenase"
                     /protein_id="HXK66508.1"
                     /translation="ALYSATKAALDGFAYSFNFEKDRNFHLVVVYPIATKTEFFKTAG
                     KGTPVPFPTQTPQKVAQEVVKGILKNKRYVFPSKIFRGMMIVNRILPFALWCYAKVEQ
                     YKFKKWVASSESK"
BASE COUNT          538 a          374 c          389 g          515 t
ORIGIN      
        1 agccatttcc accaaggatg aatcaaaaga gcgattacag gaacttgtaa aagctggagt
       61 caatgttgtg gtcatagatg cagcacaggg taactctatc taccagatag aaatgattaa
      121 atatattaaa aagaaatacc cagaacttga tgtcattgga ggtaatgttg tcaccattga
      181 gcaatgcgaa aacctgataa aggcaggggc agatgctctg cgcattggca tggggccagg
      241 ctcaatctgt actacacagg tgactatggc agttggccgc gcacaatcaa cggcagtgta
      301 tcgatgcagc aagtttgcaa aacaatacaa tataccaaca atagccgacg gcggtattgc
      361 cacaataggg cacatagcca aggcacttgc gctgggtgca tcaactgcaa tgatgggttc
      421 aatgtttgct ggcaccgaag aagcacctgg cgaatatttt tatgaaaatg gcgttcgcct
      481 taaacggtat cgtggcatgg catcactgga agcaatggaa aaaggcggtt caaagcgcta
      541 ttttgcagaa gactcaaaga tactcgttgc acagggagtt tctggcgcgg tagttgataa
      601 aggctcaatg tttgattatg tgccatatct ggtacaggga ttgaaacacg cttttcagga
      661 tatgggtgta cgcactatta aagagcttca cgaaaagcta tacaaagaag agcttcgctt
      721 tgaattgcgt tcgctgtcag cccagatcga aggtggtgtg cataccattt attcgtataa
      781 agaacccaag tatctgtaac cttcatgtta ctgatgtgat aatccgtata atgtctccgg
      841 gcgtattggc aattgcatga ggatgatgct gtaaaagcaa cgcaacaggt ctgtaccccc
      901 agctcactcc tatactgtac ataccacatg catgagccat ttcaacatcc gtagccgaat
      961 caccaatcat agcaatctgc gttggttcta attgcatggt gtgggcaata agcagtgcgc
     1021 catgcgggtc aggtttacgc ggtatggaat cctgcaaacc taaaattgtg ctgaatgcaa
     1081 tatcaggaaa atagtattta accatttcta cagtaaactc atgcgattta tttgaaaaaa
     1141 cggaaatttt tatttttttg tgcgatagtt cctttaaaag gtcatgcact cctggatatg
     1201 gttttgtttc cctgttccag ttttttgcat actcatcttt caattcggaa acaagttttg
     1261 taatctcttt atctgaacaa gtttccccaa ccgcacgctg aatgagcatt gccatgccat
     1321 cgccaataaa ctgtttatat gcctctaccg gatgtgtgcg atacccatag cgtaacagga
     1381 cattattaac acagtatgcc agatcagcta tagtatccag aagtgtccca tccaggtcaa
     1441 atatgacgcc atcaattccc atggtaatta tttgctctct gatgatgcaa cccacttttt
     1501 aaatttatac tgctcaactt tagcatagca ccacagggca aatggtagaa ttctgtttac
     1561 aatcatcatg cctctaaata tttttgaagg gaacacatat cgtttatttt tcagtatccc
     1621 tttaacaact tcttgagcaa ctttttgagg tgtttgcgtg gggaaaggaa ccggcgttcc
     1681 tttgcctgct gttttaaaaa attcagtttt cgttgctatg ggataaacca ctacaaggtg
     1741 aaagtttcta tccttttcaa aattaaatga ataagcaaac ccatctagag cagctttggt
     1801 agctgaatat aatgca
//
LOCUS       DAOSZP010000484         1815 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_48318_length_1815_cov_3.281818, whole genome shotgun sequence.
ACCESSION   DAOSZP010000484 DAOSZP010000000
VERSION     DAOSZP010000484.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1815
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48318_length_1815_cov_3.281818"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..452
                     /locus_tag="PK348_09595"
     CDS             <1..452
                     /locus_tag="PK348_09595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66509.1"
                     /translation="KRELIFWRKDVIVWPTYVENLVDLLLVIAVDKRAIGNGYLVHDG
                     ESVTLQKFCEGIANALGVKPIKTHIPYWTAYMAAVVMEAVWKLFTIEKRPLLTTYTVK
                     NLGSRLRFSIDKAKRELGWVPKISFKEGFERTMQWLKTLDLESLKIK"
     gene            488..1318
                     /locus_tag="PK348_09600"
     CDS             488..1318
                     /locus_tag="PK348_09600"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012611164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXK66510.1"
                     /translation="MESFAGKTAFITGGSSGIGLSVAKLLSKEGANIAIFARNRVRLE
                     EAMKEIEQAKKRHGQRFAWYQCDVSDNAQVKKAFTQALRQFDTCDILINCAGRAYPKY
                     FEDISYKQFEETMKINLFGIWNTCSFLVPHMKQKGGYIVNTSSLAGLVGVFGYSDYSA
                     SKFAIVGFSEVLRSELKRYNITVSVLCPPDTDTPGFEVENRTKPDETKEISKSARVMS
                     PDEVAQALLKGMKKKKFIILANFDSKLTWIAKRYAPWLVEAVMLSQIKKVQKKKGTSK
                     "
     gene            1333..1737
                     /locus_tag="PK348_09605"
     CDS             1333..1737
                     /locus_tag="PK348_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012176613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66511.1"
                     /translation="MSLKTTAYLAIVTGIGILLFWIAFFTVGLAPENPPACYFAYEHS
                     FPLPDTVLAIALIIGGILAIKQHDRALYLLLPASGGLIFLGLLDMAFNWQNGMYTITV
                     MDGVLNAFINIWCVVFGLCAILSLQREFVKKS"
BASE COUNT          556 a          315 c          422 g          522 t
ORIGIN      
        1 ttaaacgcga acttattttc tggcgtaagg atgttattgt gtggccaacc tatgttgaaa
       61 atctagtgga tttactgttg gtgattgcag tggataagcg ggccattggt aatggctatc
      121 tggtgcatga tggtgaaagt gttacattgc aaaaattttg cgaaggcata gccaatgcat
      181 taggtgtaaa accaataaaa acccatatac cctactggac agcatatatg gcagcagtgg
      241 taatggaagc cgtatggaaa ttatttacca tagaaaagcg cccgcttctt acaacgtata
      301 ctgtaaaaaa tttagggtcg cgactacgat ttagcattga taaagcaaag cgggaattag
      361 gctgggtacc aaaaatatcg tttaaagaag gatttgagcg cacaatgcag tggcttaaaa
      421 cacttgattt agaatcatta aagataaagt aaaaaaaagt aaatacacgt aaagggagat
      481 agtaactatg gaaagttttg caggtaaaac agcctttatt actggtggtt caagtggcat
      541 aggattatcg gtggcaaaac tcctttcaaa ggagggtgct aatattgcta tttttgcccg
      601 caacagagtc cgactagaag aagcaatgaa agaaattgag caggcaaaaa agcgccatgg
      661 ccagcggttt gcgtggtatc agtgcgatgt atcggacaat gcacaggtta agaaagcttt
      721 tactcaggcc ttacgtcagt ttgacacatg tgatattctc atcaattgtg ctggcagggc
      781 ctatcccaaa tattttgaag atataagcta taagcagttt gaagaaacta tgaaaataaa
      841 tttgtttggt atatggaata cctgttcttt tttagtgccg catatgaagc aaaaaggcgg
      901 ctacattgtc aatacatcat cgctggcagg gctggtaggt gtattcgggt atagtgatta
      961 cagcgcatca aaatttgcca tagttgggtt ttcggaagta ttacgaagcg agctcaaacg
     1021 gtataatatt accgtatcgg tgctctgtcc ccctgacact gatactcccg gctttgaagt
     1081 tgaaaacaga acaaagcctg atgaaaccaa agagatatca aagtcagcca gggtgatgtc
     1141 acctgatgaa gtggcacagg ccctgcttaa aggaatgaaa aagaagaagt ttataatcct
     1201 tgcaaacttt gacagtaaac tcacctggat tgccaaacgg tatgctccat ggcttgttga
     1261 agcagtgatg ctttctcaga taaaaaaggt gcaaaagaaa aagggaacat caaaataata
     1321 acaggaggtt ttatgtcatt aaaaacaaca gcatatttgg cgatagttac cgggataggg
     1381 atccttttat tctggattgc ctttttcacg gtagggcttg ctccggaaaa tccaccagcg
     1441 tgctattttg cctatgaaca ttccttcccc ctgcctgata cagtgttagc gatagctctt
     1501 attattggag gaatcctggc aataaaacaa catgacagag ctttgtatct gcttttgcct
     1561 gcatcgggtg gtttaatctt tttggggtta cttgatatgg cgtttaactg gcagaatggc
     1621 atgtatacaa tcaccgttat ggatggcgta ctcaatgcgt ttattaatat ttggtgtgtg
     1681 gtttttggat tgtgtgctat actgtcacta cagcgtgaat ttgtgaaaaa gtcttgacag
     1741 gcaataagct ctttcgtatt tacatttcat cctgaacgta atcactattt ttatgggcga
     1801 ttagctcagc ctggt
//
LOCUS       DAOSZP010000485         1803 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_48738_length_1803_cov_3.573799, whole genome shotgun sequence.
ACCESSION   DAOSZP010000485 DAOSZP010000000
VERSION     DAOSZP010000485.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1803
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48738_length_1803_cov_3.573799"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..475)
                     /locus_tag="PK348_09610"
     CDS             complement(<1..475)
                     /locus_tag="PK348_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015948097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXK66512.1"
                     /translation="MEFGFSEEQLLFRDTIYKYAKNEIVPLCEEADLKSEFSFEIWKK
                     LGQMGLLGLPFPEEYGGQGADVVTCCLAGEALGHAGVDSGHLLALGAHTYLCTDTLYK
                     HGNEAQRKKYIPKLASGEWIGCMGLTEPGAGSDAASLQTTAVKKGDRWILNGSKTF"
     gene            complement(475..1089)
                     /locus_tag="PK348_09615"
     CDS             complement(475..1089)
                     /locus_tag="PK348_09615"
                     /inference="COORDINATES: protein motif:HMM:NF019958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HXK66513.1"
                     /translation="MKIKERSDIFDIRRAQLTSATYNVVSKKGYYNFTIKDIAQEAGM
                     STGLIHYYFKNKQDLLLNLLREINRNIRTSLQHDLEHLSDPVDRLAAFIERACNLVMQ
                     EKNYFYVLLDFWTQLNHNERMRTAIQKLYTSYRDVCSEILNEGIEQGKFNNIDIRYTA
                     TMIVSMVQGLIIQYVIDPDAFDYAHYAHKIKNQIIETILNKGGQ"
     gene            complement(1144..>1803)
                     /locus_tag="PK348_09620"
     CDS             complement(1144..>1803)
                     /locus_tag="PK348_09620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66514.1"
                     /translation="FDHRVFAVADATERFPHASRLLLSNIVSGLQTIPQSSDEWLHYL
                     NGIYASQEYHLKSTLSLIALQKQESVSRAFIAHGGDSSLQIYNKQNKSLVYKTSVNMN
                     FAGRSKHVDSIDCIPLQDQLRVILYSDGFHDVIKHCNIVMEDLLHVSLIEAITHIIDK
                     VGNLDCEEYDDISLLLFDIPEIYTVNTKGILMGGTTRLQEQKVKESISIHDYNSLIRI
                     M"
BASE COUNT          547 a          391 c          272 g          593 t
ORIGIN      
        1 tgaatgtttt tgaaccgttc aatatccatc tatcgccttt tttaactgca gttgtttgta
       61 gtgatgcagc atcagaacca gcaccaggtt ctgttaatcc catacatcca atccattcac
      121 cactggctaa ttttggtata tattttttac gttgtgcttc atttccatgt ttgtatagag
      181 tatcagtaca taaatacgtg tgtgcaccta atgctaataa atgcccgcta tcaacaccag
      241 catgacccaa cgcttcacca gcaaggcaac atgttaccac atcggccccc tgaccaccat
      301 attcttccgg gaatggcaaa cctaaaagac ccatttgccc taactttttc cagatttcaa
      361 atgaaaactc tgattttaaa tcagcttctt cacacagagg aacaatttca ttttttgcat
      421 atttatatat tgtatccctg aataaaagct gctcctcact gaacccaaat tccatcattg
      481 acctcctttg ttaagtatag tttcaattat ttgatttttt attttatgtg cataatgcgc
      541 ataatcaaat gcatcaggat ctataacata ttgaataata agaccctgaa ccatggaaac
      601 aatcatggta gcggtatacc tgatatctat gttattgaat tttccctgct ctatcccttc
      661 atttaaaatt tccgaacata catcacgata gctagtatat agtttctgaa tagcagtgcg
      721 catccgttca ttatggttca gctgtgtcca gaaatccaga agcacataga agtaattctt
      781 ttcctgcatt accagattgc atgcccgttc tataaaagca gctaatctgt caacagggtc
      841 cgataggtgt tcaagatcat gctgtaatga tgttctaata ttacgattaa tctctctgag
      901 taaatttaaa agcaggtcct gtttattctt aaaataatag tggatcaacc ctgttgacat
      961 acctgcttcc tgggcaatat cctttatagt gaaattataa taccccttct tactcaccac
     1021 attgtaagta gcagaggtaa gctgtgcgcg tcgtatatca aaaatatctg agcgttcctt
     1081 aattttcatt tgaatctcca tccatatgat tgaatgacca atcaatatta tcagaaatca
     1141 tttttacatt atcctaatca aagaattata atcatggata ctgattgatt ctttgacttt
     1201 ctgctcctgc agtcttgtgg tcccacccat cagaattccc ttcgtattta ccgtatatat
     1261 ttcaggaata tcaaataaca aaaggcttat atcatcatac tcttcacagt ccaaattccc
     1321 aaccttatca ataatatgcg ttatagcttc tatcaatgaa acatgaagca aatcttccat
     1381 tacaatattg caatgcttga taacatcatg gaaaccatca ctatacaaaa taactcttaa
     1441 ctgatcttgc agtggtatac aatcaattga atcaacgtgc ttgcttcttc cggcaaaatt
     1501 catattcacc gacgttttgt acaccaggct cttattctgc ttgttatata tttgtaatga
     1561 actatcgcca ccatgtgcaa taaatgcccg tgaaacagac tcctgttttt gaagtgctat
     1621 caaactcagt gtggatttta aatgatattc ctgactggca taaattccat tcagatagtg
     1681 taaccactca tcactgcttt gaggaattgt ttgcaaacca cttacaatat ttgaaagcag
     1741 taagcgtgat gcgtgaggga agcgctctgt tgcatcagca actgcaaata ctcggtgatc
     1801 aaa
//
LOCUS       DAOSZP010000486         1800 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_48847_length_1800_cov_3.111175, whole genome shotgun sequence.
ACCESSION   DAOSZP010000486 DAOSZP010000000
VERSION     DAOSZP010000486.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1800)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1800)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1800
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48847_length_1800_cov_3.111175"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="PK348_09625"
     CDS             <1..320
                     /locus_tag="PK348_09625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66515.1"
                     /translation="VCLAYLNILYFQNIKKRKTQSIALLVKKLDDIDKKLTELSVTKN
                     LVGLTIQHIIHTVTEGYEIDESVTSEQEKVALKSLYLYKGMLEGLQYSTRVINNMIVL
                     LQQ"
     gene            complement(322..1077)
                     /locus_tag="PK348_09630"
     CDS             complement(322..1077)
                     /locus_tag="PK348_09630"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HXK66516.1"
                     /translation="MYPILFQYKFITIGGYGVMLAIGIYMAYILFERELILRNINPDL
                     AYKILLVAIPAGIIGSKVFHILDHMDEFLSDPAGTVFSGAGLSIYGGFLFALLFSYFL
                     IKKSNEPVLKILDITAPCIAVGYGFGRIGCHVAGDGCYGIETSSIFGVAYPNGIVPIS
                     YAVYPTPLFESFFSFIVAILLLQLRKKEFPQGTLFFIYLILNGLPRFFVEFIRRNPVV
                     FAGLTQAQIIALGLIMAGIAGLVIVHQKKLRNA"
     gene            complement(1292..>1800)
                     /locus_tag="PK348_09635"
     CDS             complement(1292..>1800)
                     /locus_tag="PK348_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003929040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HXK66517.1"
                     /translation="MDDDGNFVPVGEVGEIVHRSGHVMTGYLDDPEKTAEAFAYDWFH
                     SGDLGRFDEDGYLYVVDRKKDMIKTGGENVASREVEEVLYQHPAIAEVAVFGLPDPKW
                     IELVCAAVVLKKDMQATEEEIIAFAKQHLAGFKTPKKIFIMNALPKNPSGKILKRELR
                     QTLAVETK"
BASE COUNT          595 a          355 c          293 g          557 t
ORIGIN      
        1 ctgtttgttt agcctaccta aatatattat acttccaaaa cataaaaaag agaaaaactc
       61 aatctatagc tttgctggta aagaaactgg atgatattga taaaaagctt actgaactca
      121 gtgttacaaa gaatttggta gggttaacca tacagcacat tattcataca gttactgaag
      181 gctatgaaat tgatgagtct gttaccagtg aacaggaaaa agtagcttta aaatccctgt
      241 acttatataa ggggatgctt gaagggctac agtacagcac ccgtgttata aacaatatga
      301 tagtattatt acaacaatag tttatgcatt ccgtaacttt ttttgatgaa caatcacaag
      361 cccggcaatg cctgccataa tcaaccctaa tgcaattatt tgtgcttgtg ttaagccggc
      421 aaaaacaacc gggtttctgc gaatgaattc cacaaaaaac cgtggcaatc cgttcaaaat
      481 cagatatatg aaaaaaagtg ttccctgtgg aaattctttt ttcctgagtt gtaataaaag
      541 tattgccaca ataaatgaga aaaatgattc aaaaagaggt gtggggtaca ccgcgtagga
      601 tatcgggaca atcccattgg gataagcaac accaaaaatg cttgatgttt caataccata
      661 acatccgtca ccagcaacgt ggcatcctat tcttccaaaa ccatacccaa ctgctataca
      721 gggtgctgta atatcaagaa tttttaaaac aggctcatta cttttcttaa tgagaaaata
      781 gctgaataac agtgcaaaga gaaatccacc atatatagaa aggccagcac ctgaaaatac
      841 cgtacctgca ggatctgata aaaattcatc catatgatcc agtatgtgaa aaacttttga
      901 accaataata ccagctggaa ttgctaccag taatatcttg tatgctaaat ccgggttgat
      961 attgcgtaag ataagttccc gttcaaacaa aatgtatgcc atataaattc ctatagcaag
     1021 cattacaccg tatccaccaa tggtaataaa cttatactgg aatagtattg gatacatggt
     1081 gaagttcctc cttttcattt aattaatgct attttgactt tatttaaagc tttctaaaaa
     1141 agatattatg atagtacatc tattgaatta ttttcagaac tgttgagtgt actaaagctt
     1201 taatacaaaa agtcatattg cttacaacaa aaaaaattgc aacagaatta tatcttctgt
     1261 tgcaattttt tgtgctacta ctgggtattg tttactttgt ttccactgct aatgtctgac
     1321 gtaattcccg ttttaatatt ttgcctgatg gatttttggg caatgcattc atgataaaga
     1381 ttttttttgg cgttttgaac cctgctaaat gctgtttggc aaatgcaatg atctcttcct
     1441 ctgttgcctg catatctttc ttcaatacca ctgctgcgca tacaagttca atccatttgg
     1501 gatcgggcag tccaaataca gctacttcag caatggcagg atgctggtat aatacttctt
     1561 caacttcacg tgatgcaaca ttttcgccac ctgtctttat catatctttt ttacggtcaa
     1621 caacatacaa atatccgtct tcatcaaatc gtcctaaatc accactatga aaccagtcat
     1681 atgcaaatgc ttcggcagtt ttttcaggat catccagata ccctgtcata acatgaccag
     1741 atctgtgaac tatctcacct acttcaccaa ctggtacaaa gttgccatca tcatccatca
//
LOCUS       DAOSZP010000487         1799 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_48874_length_1799_cov_2.910550, whole genome shotgun sequence.
ACCESSION   DAOSZP010000487 DAOSZP010000000
VERSION     DAOSZP010000487.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1799
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48874_length_1799_cov_2.910550"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            263..1135
                     /locus_tag="PK348_09640"
     CDS             263..1135
                     /locus_tag="PK348_09640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66518.1"
                     /translation="MINKISQLLIIATIMISTNAFALDLNVGATLWYANWKPHWEKEF
                     RDIFRDESGEKTHFKMDDTYLIGPIVSLKLGDTFSLGANMIYGKYTASCIKMWYEGGT
                     ESIHSTMDITKYDTDIILNYRAFTYVQFFVGLKFQHYNLTETQRGIYDTPPGPFSREL
                     LSNSYGPGLGIGLTIPLDENFAMLLNISGIYLRTKVEDSWKENSGANPPSYIYTLNAY
                     GINNSLSLAYFLPSFNITLLTGFRYQYLKYKQADVKKTNYDPGDEFRFDRDYNDQRDI
                     FWGINISVLYSFSI"
     gene            complement(1146..>1799)
                     /locus_tag="PK348_09645"
     CDS             complement(1146..>1799)
                     /locus_tag="PK348_09645"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HXK66519.1"
                     /translation="PTIAIIDPELMVGCPAGVTAASGLDALTQLIESYVSTQANPFTD
                     ALAGSGIAKIGHSLLPLCIHHEDTVFHRANMAYGAFISGITLAHAGLGVVHGLASPLG
                     ALFNIPHGVACGTLLALATKATIKKLQVLGDSYYIKKYAAISELLTGHKSTSDQEACS
                     MLIATLEEWVDILHIPRLSEYGVREVDFAAIIKEGSNKNNPAPLNEQEIMLMLQERL"
BASE COUNT          590 a          329 c          309 g          571 t
ORIGIN      
        1 aagaatgttc acgaatagga taataagagc ctatttacaa taacaaatta tgatttaatt
       61 ttatattgac atttgcaaag attcatttat aattcttaca atatatgttt attatattat
      121 agtatactat aatataaaga tataatttca gattgggcac attccacacc gtaaaccatt
      181 ctgttaatat tctgtattat acaataatca aataaaataa tattcactat tgacttaaat
      241 tttcagatga aggggatacg ttatgataaa taaaatatca caacttttaa ttatagctac
      301 tatcatgatt tccactaatg catttgcatt agatttaaat gttggtgcaa cattatggta
      361 tgctaactgg aaacctcact gggaaaaaga atttcgggat atattccgtg atgaatccgg
      421 tgaaaaaaca cattttaaaa tggatgatac ttatttgatt gggccaatag tgtcattaaa
      481 acttggtgat acatttagcc tgggtgcaaa catgatctat gggaaataca ctgcttcatg
      541 tattaaaatg tggtatgaag gaggcacaga atcaatccat tcaacaatgg atatcacaaa
      601 atatgataca gatatcatac taaattacag agcatttact tatgtacaat tttttgttgg
      661 gcttaagttt caacactata atcttactga aacacagaga ggaatatatg atactcctcc
      721 aggcccattt tcccgtgaat tattatcaaa cagttatggt ccgggtttag gcattggatt
      781 gacaattcct ttagatgaaa attttgccat gcttttgaat atttcaggaa tatatctgag
      841 aacaaaggtt gaagattcgt ggaaggaaaa ctcaggtgca aatccaccat catacatata
      901 cacgttaaat gcctatggca ttaataattc attgtcatta gcctattttc ttccctcatt
      961 taatataaca ttactcacag gttttagata tcaatatctt aaatataaac aagctgatgt
     1021 taaaaaaacc aactatgatc caggggatga attcagattt gatagagatt ataatgatca
     1081 gagggatatt ttttggggta taaatatttc tgttttatat agtttctcta tctaagaaaa
     1141 aacttttata atcgttcctg taacatgagc attatttcct gttcattaag tggtgctgga
     1201 ttattcttat tgctaccctc cttaattatc gcagcaaaat ctacctcgcg cacaccgtat
     1261 tcagacaggc gaggtatgtg caatatatca acccactctt ctaatgtggc aataagcata
     1321 ctgcaggctt cctggtcact ggtgctttta tgcccagtta aaagctcact tatagcagca
     1381 tattttttta tatagtaact atcgcccaat acttgtaatt tttttattgt agcttttgtt
     1441 gctaaagcta acagtgtccc gcaggcaacg ccatgaggaa tattaaacaa tgcaccaagc
     1501 ggtgacgcca acccatgcac cacgccaagc cctgcatgcg caagcgttat gcccgatata
     1561 aatgcgccgt acgccatatt agcgcgatgg aacaccgtat cttcatggtg aatacatagt
     1621 ggcaacaaac tgtgcccaat ctttgcaata ccacttccgg ccagtgcatc ggtgaaagga
     1681 tttgcctgtg ttgacacata cgactcaatc aattgcgtaa gcgcatcaag tccgcttgct
     1741 gcagtgactc cggcagggca cccaaccata agctctggat caatgatagc aatagtggg
//
LOCUS       DAOSZP010000488         1783 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_49460_length_1783_cov_2.550926, whole genome shotgun sequence.
ACCESSION   DAOSZP010000488 DAOSZP010000000
VERSION     DAOSZP010000488.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1783
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49460_length_1783_cov_2.550926"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..107)
                     /locus_tag="PK348_09650"
     CDS             complement(<1..107)
                     /locus_tag="PK348_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015420130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXK66520.1"
                     /translation="MKKLPIGIQSFVEIRTDNYYYADKTFYVEKLTNSG"
     gene            complement(618..1208)
                     /locus_tag="PK348_09655"
     CDS             complement(618..1208)
                     /locus_tag="PK348_09655"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012083271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HXK66521.1"
                     /translation="MNIALFDFDGTITKKDTLFDFIIFAVGYKTFIIKLISLVPLLIL
                     YTLKIIPNWYAKEKLLAAYFKGWELKNFDTIAHKYAQERISSITKEEAIKAIEKHKVN
                     GDLIVVVTASCQNWVKPWCDEHKIDCVATRLEIKNGKLTGRFDGKNCYGKEKVNRIKE
                     KYNLSNYKKIYAYGNSKGDKEMLDLAQVRVYKWVTL"
     gene            complement(1211..>1783)
                     /locus_tag="PK348_09660"
     CDS             complement(1211..>1783)
                     /locus_tag="PK348_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012083272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXK66522.1"
                     /translation="VPVTFDAVKYNTHKAFYNKLSPKPDGVLCVFGYLGDNIKAIQDF
                     TEAERIIDVNYKGAVSILNIVSHDFMQRKSGWIAAISSVAGDRGRQSNFIYGSAKGGL
                     SVYLSGLRNLLCKHNVQVLTIKPGFVNTKMTRGMDLPKKLMAEPEDVAEDIFKAWKKK
                     KDVLYTKWIWRYIMLIIIHIPERIFKRLSL"
BASE COUNT          519 a          366 c          255 g          643 t
ORIGIN      
        1 ttacctgaat ttgttagctt ctctacataa aaagttttat cagcatagta ataattatct
       61 gttcttatct ccacaaaact ttgtatacct atggggagtt ttttcatttt gtattacctt
      121 aattttataa atactttaaa ggctaaatgt tactctgtat tcataaaaat ttttatttgc
      181 ataaaataat caggtagtct ataggatttg tccacaactt tttgtggtta ttcttttctc
      241 ttggatagca ctatcacatc attgcacatc cattgttcat atctgttatt gtaagcctaa
      301 ggagtagcca tctctttcca caaatatggg attgcttcgt tgctgcacac ctaagaatga
      361 cacctttatc ctgagggtag cacgtcatcc tgagccaagc gaaggacccc cttatcccct
      421 caagagattc ttcccttcga caagctcaga gcaagtgctt ctgctcaaaa tgaccgtgac
      481 agtgcgtcat tgcaagttcc aaagtggcgt ggtaatcccc ggtgatgtta aatatttttt
      541 ttgcctgcaa tattgcaact gtgataattg actattttta catgatttaa gttgtctttc
      601 agtaacccac aaatccatta caaagttacc cacttataaa ctcttacttg cgccagatca
      661 agcatttctt tatcaccttt gctattaccg taagcatata tttttttgta attgctgaga
      721 ttatattttt ctttgatgcg atttaccttt tcctttccat aacaattctt cccatcaaat
      781 ctccccgtga gcttaccatt ttttatttct aaacgtgtag ctacacaatc aatcttatgc
      841 tcatcacacc agggctttac ccaattttga caggaggcag ttaccactac aataagatct
      901 ccatttacct tatgtttttc tattgctttt attgcttctt cttttgtaat tgatgaaatt
      961 ctttcttgtg catatttgtg ggcaatagtg tcaaagtttt tcaattccca acctttgaaa
     1021 tatgcagcaa gcaatttttc ttttgcatac caatttggta taattttcaa tgtatagagt
     1081 ataagcaatg gcaccagtga tataagtttt attataaaag ttttatagcc aacagcaaaa
     1141 atgatgaaat caaaaagcgt atcttttttt gtaatggtac catcaaaatc aaataatgca
     1201 atattcatag ttacagtgat aatcttttaa aaatccgctc gggaatatgt ataatgatta
     1261 gcataatata gcgccatatc cattttgtat ataatacatc ttttttcttt ttccatgcct
     1321 taaaaatatc ttcagctaca tcttctggct cggccattag ttttttgggt aaatccattc
     1381 ctcgtgtcat cttagtattc acaaatccag gctttatagt taatacctgc acattgtgtt
     1441 tgcaaagcaa atttcgcaaa cccgaaagat atacgctcaa tcctcctttt gcactgccat
     1501 agataaagtt gctctgcctg cctctatccc ctgcaactga tgatattgct gcaatccatc
     1561 cacttttgcg ctgcataaaa tcatgtgaaa caatattcaa tattgaaacc gctcctttgt
     1621 aattcacatc aatgatgcgc tcagcctcag taaaatcctg tatagccttt atattgtcac
     1681 ctaaataacc aaaaacgcaa aggaccccat caggttttgg cgatagttta ttataaaaag
     1741 ctttatgtgt gttatattta accgcatcaa aagtaacagg tac
//
LOCUS       DAOSZP010000489         1769 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_49946_length_1769_cov_3.699533, whole genome shotgun sequence.
ACCESSION   DAOSZP010000489 DAOSZP010000000
VERSION     DAOSZP010000489.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1769)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1769)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1769
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49946_length_1769_cov_3.699533"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(158..1219)
                     /locus_tag="PK348_09665"
     CDS             complement(158..1219)
                     /locus_tag="PK348_09665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66523.1"
                     /translation="MVKKILMAIACSMLISCGYFRVTDLSANRVCAIPSGAALGAVSI
                     NYNESGLLDMSFGVLLADDKVYVKDNIQKKVLVFDRDGKCLIAIGNVSATAEGKDIKK
                     TQVKFDIIGTMVADSDGNLYVQNRLASVQRIDAAGTQIDMSPSFILVFDEEGKLQYTI
                     GQTGMPTVPFYYIEHLEIDKKNRLFVIARTYDIWSVYRFDKRQRDYYMTFSRNYFVDT
                     VDATTSYTGKIENIRILHSGDSFLLSVAYYNNTRFKYRKLYQFDIKESKPVELLKISE
                     PKNELFTVAQNKYIYVWNVEETNEVKFMLYNFDGTIIGNVRCKFPGQALFSDIDITSE
                     GKLYSYHAYSTGLEVYEWK"
     gene            1413..>1769
                     /locus_tag="PK348_09670"
     CDS             1413..>1769
                     /locus_tag="PK348_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007493351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXK66524.1"
                     /translation="MDFNLSEEQQMVKDMCRKFAENELAPKAEHYDRTHEFPWEHVKK
                     LGEMGMMGVVYPEEYGGSGMDYLTYAIAVEEISRGCASTGVIVSAHNSLCLSPIYYFG
                     TEQQKKKYLPKLCSGEW"
BASE COUNT          533 a          373 c          305 g          558 t
ORIGIN      
        1 agaaaactgc aacattatgc gctttaacag tatgcaatac ataatcagca actgacttac
       61 ctgccagtgc catcagcact tcaccgggta ttccatactg ctgtatggta atcctgtcaa
      121 tttcctgcat ttgctgcgct gaaactacct tcatgcatca cttccattcg tatacttcta
      181 accctgttga gtaggcatgg tatgaatata atttaccctc actggtaata tcaatatccg
      241 aaaataatgc ctgccctggg aatttgcacc gcacattccc aattattgta ccgtcaaaat
      301 tatacagcat aaactttact tcgtttgttt cttcaacatt ccacacatag atatatttat
      361 tctgtgctac cgtgaataat tcattctttg gttctgaaat ttttaacaat tcaacaggtt
      421 ttgattcttt aatatcaaac tgatataact ttcggtattt aaaccgggtg ttattgtaat
      481 aggcaacaga aagcaagaaa ctatcgccgc tatgtaatat cctgatattt tctatttttc
      541 cggtatatga tgttgtggca tcaactgtat ctacaaagta atttctggaa aatgtcatat
      601 aataatcccg ctggcgtttg tcaaagcgat acacgctcca tatatcatac gtacgtgcaa
      661 taacaaaaag cctgtttttt ttgtctattt ctaaatgctc aatgtagtaa aaaggaactg
      721 ttggcattcc cgtctggcct atggtgtatt gcagtttacc ttcttcatca aagacaagta
      781 taaaagaagg tgacatatca atctgcgtcc ctgcagcgtc aattcgctga actgaggcca
      841 gcctgttttg tacatacagg ttgccatctg aatcggcaac catagtgcct atgatgtcaa
      901 atttaacctg tgtttttttt atgtcttttc cttctgctgt tgcactgaca ttgccaatag
      961 ctattaaaca tttcccatcc ctgtcaaaaa caagcacttt cttctggata ttatccttaa
     1021 cataaacttt atcatctgcc agaagcacgc caaaactcat atcaagaagt cctgactcat
     1081 tatagttaat gcttaccgca cctaatgccg ctcctgacgg aattgcacaa accctgtttg
     1141 ctgaaagatc ggtcaccctg aaataaccgc atgaaataag catgctacag gctattgcca
     1201 tcaatatttt tttaaccata ctgttcatcc ctcatcagta actatcgcca cgtatatact
     1261 gtaaccattt gtagtatgta ccagcctttt tttcaacata aaaaatccat gctttttacc
     1321 tgtcaattgt tattgccttg acaaaataaa tccatatatg aaacgatagt acacagcaaa
     1381 tatttataga gatagtataa ggagatcggc ttatggattt taatttatct gaagaacaac
     1441 agatggtcaa agatatgtgc aggaagtttg cagaaaacga acttgcacca aaagctgaac
     1501 attatgaccg aacgcatgaa tttccatggg aacatgtaaa aaaattaggt gaaatgggga
     1561 tgatgggtgt tgtttaccca gaagaatacg gtggatcggg tatggattac cttacctatg
     1621 ccattgccgt tgaagagatt tcgcgtgggt gtgcatccac gggagttatt gtatctgccc
     1681 ataactcatt gtgcctgtca cctatttatt actttggcac tgaacagcag aaaaagaaat
     1741 acctgccaaa gctatgctca ggtgaatgg
//
LOCUS       DAOSZP010000490         1765 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_50092_length_1765_cov_2.995906, whole genome shotgun sequence.
ACCESSION   DAOSZP010000490 DAOSZP010000000
VERSION     DAOSZP010000490.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1765
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50092_length_1765_cov_2.995906"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1702
                     /locus_tag="PK348_09675"
     CDS             <1..1702
                     /locus_tag="PK348_09675"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HXK66525.1"
                     /translation="LDTVPAQDVALQIGEIMNADRAYCNGVFIGASGRFPPHFKSAWN
                     QFRHYTIPATLLHKGKNLLSIKIYYDAEFWINAPLRMVSDSSGKTAFDFINFMRVHLM
                     QAMFFIMLTLAIFFIVMFYYQRDSVEYLFFGLACLSVSFPQLLHFIENLYPDVPYSSN
                     TVLKVSQPGLIFFPALLNLFYRYHVGRNKLWITSIYLLIPVVLTVVLIFQKTRSDILF
                     IRNLSIVVSILFMIDIFVTSGLQLMQHKKAGLLIFIGIVPMVILGLHDVLAFGFKIIP
                     SDVVLYVYGLPFLLFIIAIYLVNIFITALQQSKELNVQLQQLLQDARKLAYLEKELEI
                     ARTIQNQLLPSEIPHLKGMDIAIRFQPALRIGGDIYNIFQLKDGVVIFLADVVGHGIP
                     AAMIASMVNILFKMFQMLSVDPGLLLRSLNINICNMLPQTFVTAACLYINRYEKFVRI
                     ARAGHPSIIIQKADGNIVEYMPQGKALGLKTDNKYETLNQAVSPGDKILIYTDGIVEA
                     IDINKNMFGSERLINIVQQTKEASCAESIEAIYSELIHFVGDTSALEDDVTLIMIRIE
                     "
BASE COUNT          511 a          296 c          345 g          613 t
ORIGIN      
        1 gctggataca gtacctgcgc aggacgtggc acttcaaatt ggtgagataa tgaatgctga
       61 tagggcatat tgcaatggag tctttatagg tgcaagcggc aggttcccac cacattttaa
      121 atcagcatgg aaccagttca ggcattatac aatacctgct acgctgttgc acaaaggcaa
      181 aaacctttta tccataaaaa tatactatga tgctgaattc tggataaatg ctcctctgcg
      241 gatggttagc gatagttccg gtaaaacggc ttttgatttt attaacttta tgcgtgtaca
      301 tcttatgcag gcaatgtttt ttatcatgct gacactggct atattcttta ttgtgatgtt
      361 ttattatcaa agggattcgg tggaatatct attctttggg cttgcgtgtt tatcggtttc
      421 attccctcag cttcttcatt ttattgaaaa cttatatcct gatgtgccgt attcatccaa
      481 tactgtgcta aaggtttcac agccagggtt gatatttttc ccggcattgt taaacctgtt
      541 ttatcggtat catgttggaa ggaataagct ctggataact tcaatctatc ttttaattcc
      601 tgtagtactt acagttgtat tgatttttca gaagacacgc agtgatattt tatttatacg
      661 aaacctttca attgttgtat caattttgtt tatgatagac atatttgtaa cctcaggctt
      721 gcaattaatg caacataaaa aggccggttt gcttatcttt attggtatag tccctatggt
      781 catattagga ctgcatgacg tacttgcttt tggttttaag attattcctt cggatgttgt
      841 attatatgtg tatggcctgc cttttttatt atttatcatt gcaatttacc ttgtaaatat
      901 ttttattaca gcattgcaac aatcaaaaga attgaatgtc cagctacagc aattgttgca
      961 ggatgcaaga aaattagcat atctggaaaa agagcttgaa attgctcgta ctatacaaaa
     1021 tcaattactt ccatctgaaa tcccccatct taaggggatg gatatagcaa tacggtttca
     1081 accggcctta cgtattggtg gagacattta caatattttt cagcttaaag atggcgttgt
     1141 catattcctt gctgatgtgg tggggcatgg tattccagcg gctatgatag cctccatggt
     1201 gaatattctt tttaagatgt ttcagatgtt gtctgttgat ccggggttgc ttttgcgaag
     1261 cttgaatatt aatatatgca atatgctacc gcaaaccttt gttacagcgg catgtttgta
     1321 tatcaatcgc tatgaaaaat ttgtgcgcat tgcacgtgct ggccatccat caattattat
     1381 tcagaaagct gatggtaaca ttgtagaata tatgcctcag ggtaaagcgt taggattgaa
     1441 gactgataat aaatacgaaa cgttaaatca agcagtatca ccgggtgata agattttaat
     1501 atacactgac ggtatagttg aagctataga tattaataaa aatatgtttg gctctgaacg
     1561 attgattaac attgtccagc aaacaaaaga agcatcatgt gctgaatcaa ttgaggctat
     1621 ctattctgaa ctcatacact ttgttggtga tacctcagca cttgaagatg atgtaacgct
     1681 gattatgata aggattgagt aaaactatcc agaagatttt catttagaag ttttaaactc
     1741 catagtattt tacctgttaa aaaat
//
LOCUS       DAOSZP010000491         1764 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_50136_length_1764_cov_2.754827, whole genome shotgun sequence.
ACCESSION   DAOSZP010000491 DAOSZP010000000
VERSION     DAOSZP010000491.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1764
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50136_length_1764_cov_2.754827"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1625)
                     /gene="ilvD"
                     /locus_tag="PK348_09680"
     CDS             complement(<1..1625)
                     /gene="ilvD"
                     /locus_tag="PK348_09680"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HXK66526.1"
                     /translation="MISDRVKKGIEKAPHRSLFKALGLNDDELAKPLIGVVNSFNEII
                     PGHIHLRNIVDAVKKGVIAAGGTPIEFPTIGVCDGIAMNHIGMKYSLGSRELIADSIE
                     IMAKGHAFDGLVMVTNCDKIVPGMIIAAVKLDIPAIIVSGGPMLPGMYKGKPIAVDNV
                     FEAVGKAAKGTITKEELYEIECAACPGAGSCAGLFTANSMNCLTEALGLALPYNGTIP
                     AVFAERIRLAKESGKKIIELVKKQITPRKIVTKKAIENALAVDMAIGGSSNTALHIPA
                     IAHYAGIPIRLKDIQPISERTPHLCSIAPSGPHHMIDLHYAGGIPALMNELLKHGLIH
                     GTCMTVTGKSIAQNVKDKSILDTSVIRLVTKPVHKDGGLAVLTGNLAPDGCIVKQAAV
                     DPSMLKHSGPAKVFDSEEEAVKAIMGNQIQKGDVIVIRYEGPRGGPGMREMLTPTSVI
                     AGMGMDKEVALITDGRFSGATRGSSIGHISPEAAAGGPLAIVKDGDIIEIDIPAKKLT
                     LKVNDNEIKARLAKWKAPKPKITTGYMYRYSLMV"
BASE COUNT          479 a          393 c          331 g          561 t
ORIGIN      
        1 accatgaggc tatagcggta catatatcca gtagtaatct ttggttttgg tgctttccat
       61 ttggccagcc gtgcttttat ttcgttatca tttactttta acgtaagttt tttagcaggg
      121 atatcaattt caataatatc accatcttta acaatggcaa gtggaccacc agcagcagct
      181 tcaggtgaaa tatgaccaat agatgatcca cgtgttgctc cagaaaatct gccatcagtt
      241 atcagtgcaa cctctttatc cattcccata ccagcaataa ctgatgttgg tgttagcatt
      301 tcacgcattc caggccctcc ccgcggtcct tcgtaacgaa taactatgac atcacctttc
      361 tgtatctggt ttcccatgat ggctttaact gcttcttctt ctgaatcaaa gacttttgca
      421 ggcccactat gtttcaacat agatgggtca acagcagcct gttttactat acagccatca
      481 ggtgccagat tcccggtaag tactgcaagt ccgccatctt tatgaacagg tttagtaact
      541 aatcgtatta ctgaagtatc cagtattgat ttatctttta cattttgtgc tattgatttg
      601 ccagtaactg tcatgcaggt gccatgaata agcccgtgtt taagcagttc attcattaat
      661 gccgggatac caccagcata atgcaggtca atcatgtggt gtggtcctga tggtgcaatg
      721 ctgcataaat gaggtgtacg ctcagaaatg ggctgaatgt cttttagtct gatagggatt
      781 cctgcataat gagctattgc cggaatatgc aaagcggtat ttgaagagcc ccctattgcc
      841 atatcaacag caagtgcatt ttcaatggct tttttagtta ctatcttgcg gggagttatt
      901 tgctttttaa caagttcaat tatcttcttt ccactttcct tggcaaggcg aatacgttcg
      961 gcaaaaactg caggtattgt gccattatag ggaagagcaa gtcccaaggc ttcagtaaga
     1021 caattcatgg aattagcagt gaaaagccct gcacaggagc ctgcgcccgg gcatgctgca
     1081 cattctattt catataattc ttcttttgtg atagttcctt ttgcagcttt gccaactgct
     1141 tcaaatacat tgtcaactgc aataggcttg cctttataca ttccaggaag cattgggcca
     1201 ccagacacaa taattgctgg tatatctaat ttcacagctg ctataatcat accggggaca
     1261 attttatcac aattggttac cataacaagg ccatcaaacg catgcccttt tgccataatt
     1321 tcaattgagt cggcaataag ttcacgtgat cctaatgaat atttcatgcc aatgtggttc
     1381 atggcaatgc catcacacac acctattgta gggaattcaa tcggcgtgcc accagcagca
     1441 atgacacctt ttttaactgc atctacaata ttgcgtaaat gtatatgtcc cggaattatc
     1501 tcattaaatg agttaacaac gccaattaat ggttttgcta attcgtcatc atttaaccct
     1561 aacgctttaa acagtgagcg atgaggagct ttctcaattc ctttcttaac gcgatcactt
     1621 atcatgaaac attcctcctg taaaaattga atttataatt agtgtcattt cgaatccctt
     1681 actaccaacg ttgtcatttc gaatccctga ataccaacgc tgtcatttcg aatccccgaa
     1741 ggggtgagaa atctttcttg tctg
//
LOCUS       DAOSZP010000492         1761 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_50247_length_1761_cov_2.618992, whole genome shotgun sequence.
ACCESSION   DAOSZP010000492 DAOSZP010000000
VERSION     DAOSZP010000492.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1761)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1761)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1761
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50247_length_1761_cov_2.618992"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..466)
                     /locus_tag="PK348_09685"
     CDS             complement(<1..466)
                     /locus_tag="PK348_09685"
                     /inference="COORDINATES: protein motif:HMM:NF014460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXK66527.1"
                     /translation="MNGTEITIPRTVVQFFESVGKHSLFFTQTVKWIFKPPFDAKNIL
                     TQMLEIGYKSLPVTCITLFFTGMVMALQIGKAMDSLMSGSSVFIGSAVSIAMFRELCP
                     VLTALLLSGRVGSAIAAEIGTMRVTEQIDALITLDANPIQYLSVPRFLACLVM"
     gene            complement(468..>1761)
                     /gene="dnaB"
                     /locus_tag="PK348_09690"
     CDS             complement(468..>1761)
                     /gene="dnaB"
                     /locus_tag="PK348_09690"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015256528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HXK66528.1"
                     /translation="AEAACLSSILLSKDALLKVIEILQVEDFYIDAHRMIYEAIRELE
                     IKAIPIDLITIKQRLIDKKQFEKAGGDPALVNIYRTVSTAANAEFYANRVKELSLRRK
                     LIETCTTIAEKSYDTTLDTKILLDDAEKDIFLITQRRITSDFESLASVLKQTLEDIGH
                     MYETKRPVTGIASGFTDLDAMLTGFHESELIIIAARPSMGKTALALNIANHVALREKQ
                     PTLFFSLEMPATQLATRILCIESMIDMQHVRTGQISSEQLRKLIDVSGRLEKSPMFID
                     DTPGLSIFELRAKARRFAQKQKVGLIVVDYLQLIVSNNRLERHQQIAEISNALKQLAR
                     ELSVPVIALSQLSRAVESRTDQKPQLSDLRESGAIEQDADVVLFIYREEKVKKETERI
                     GIADIIVAKQRNGPTGTIELRFWDKYMKFGNVEKFRED"
BASE COUNT          481 a          396 c          359 g          525 t
ORIGIN      
        1 tcataaccag gcaggctaaa aaacgtggta ccgacagata ctgaattgga tttgcatcaa
       61 gtgtgatgag cgcatcaatc tgctctgtga cacgcatggt gccaatttca gcagctatgg
      121 ctgaaccaac ccttcctgat aaaagcaaag cggtaagcac cggacacaat tctctaaaca
      181 ttgcaattga tactgcgctt ccaataaata ctgatgatcc tgacataagg gaatccatgg
      241 ctttgcctat ctgcaatgcc ataaccatgc cggtgaaaaa gagcgtgata caggttaccg
      301 gcaaggactt gtaccctatt tcaagcatct gtgttagaat attttttgca tcaaatggcg
      361 gcttaaaaat ccattttaca gtttgggtaa aaaacagtga atgcttgcct actgattcaa
      421 aaaattgaac aaccgttcgt ggtattgtta tttcagtgcc attcatatca gtcttcacga
      481 aatttttcaa cattcccaaa cttcatatac ttgtcccaga agcgtaactc aattgttccg
      541 gttggaccat tacgctgttt tgccacaatg atatcagcta tacctatacg ttcagtttct
      601 tttttaactt tttcctcacg gtatataaat aacacaacgt cagcgtcctg ttcaattgct
      661 cctgattcac gcaggtcaga aagctgtggt ttctggtcag tacgtgattc cacagcacgt
      721 gatagctgtg acagtgcaat aaccggaacc gatagttccc gggcaagttg cttcaaggca
      781 ttactgattt cagctatctg ctgatggcgt tccagtctgt tattggacac aatgagctgg
      841 agataatcaa caactatcag tccaaccttc tgtttctggg caaaacgccg tgcctttgcc
      901 ctgagttcaa aaatacttaa tcctggtgtg tcatcgatga acatggggga cttttccagc
      961 cttccggaca catcaatgag ctttcgcaac tgttcactgc ttatctggcc agttcgtaca
     1021 tgctgcatat caatcattga ttcaatacac aatattcggg ttgcaagctg tgttgcaggc
     1081 atttcaaggg agaagaacaa tgttggctgc ttttcacgca aagctacatg attggcaata
     1141 ttcaatgcaa gcgccgtttt ccccattgat ggacgggcag caatgataat aagctctgat
     1201 tcatgaaaac cggtaagcat ggcatccaga tcggtaaatc cgcttgcaat accagtaaca
     1261 ggcctttttg tttcatacat gtggcctatg tcttcaagtg tctgcttcaa cacacttgcc
     1321 aggctttcaa aatcacttgt tatgcggcgc tgcgtaatga ggaatatatc cttttcagca
     1381 tcatcaagaa gtatttttgt gtccagggtg gtatcgtatg atttttcagc tatggtggta
     1441 caggtttcta tgagctttcg ccgcaggctt aattctttaa ccctgttggc ataaaactca
     1501 gcattggctg cagtagaaac cgtacggtat atattaacca gtgcagggtc gccaccagcc
     1561 ttctcaaact gctttttgtc aatcagccgc tgctttattg taataaggtc aataggtatg
     1621 gcttttattt ctaattccct gatagcctca taaatcattc ggtgggcatc aatgtagaaa
     1681 tcctccacct ggaggatttc aataaccttt aataaagcat ctttgcttaa tagtatggaa
     1741 gataagcacg cagcttcggc c
//
LOCUS       DAOSZP010000493         1759 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_50316_length_1759_cov_3.186033, whole genome shotgun sequence.
ACCESSION   DAOSZP010000493 DAOSZP010000000
VERSION     DAOSZP010000493.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1759)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1759)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1759
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50316_length_1759_cov_3.186033"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..484)
                     /locus_tag="PK348_09695"
     CDS             complement(<1..484)
                     /locus_tag="PK348_09695"
                     /inference="COORDINATES: protein motif:HMM:NF013871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HXK66529.1"
                     /translation="MKTYTVTLAIPPRVAKEIKAICTTLTSNGHECYLIGGSVRDLIL
                     GIPVYDYDFATNAHPEDVMKLFKKVVPTGIKHGTVTVLMHYAQYEITTYRADGKYTDG
                     RHPDTVVFSKTLKEDVERRDFTINGLAYDINTSKIIDYVGGMDDIEKKIIRTIGDPLQ
                     R"
     gene            complement(481..>1759)
                     /locus_tag="PK348_09700"
     CDS             complement(481..>1759)
                     /locus_tag="PK348_09700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66530.1"
                     /translation="CTFETPKGSALLATLPGSRYPYVYPRDSACATRMLYRIAMSRLR
                     SKDIAFRLLAGIAKFIYNCQRDDGYWGQRYGLDAADKSIYKQEDNVAHGVSILCRYLL
                     AAKNRGHIPHDNEAYIEAIYKGAMFAVKRYYRNEIHLFYSTTSIHESAIEEGYSIWVN
                     YAYYCMLLLIEQVAKEFNCETKFEKAFVLKEGFGTIVKNVFTIGDRLIRRLKPDGEAD
                     LRPDITLMSPFFFGTGIVPDIFKADEIFINTMKFLEDTLWDPDLGMLQRYLPFIEDPN
                     THVHAGNGPWVQYTAMLAQYYYFTGNVEKGDTIMDIIDSYSTDGYLCEHLTTPDRFHE
                     FMTLEWLPGSDVNKEFAPDIMVDGITYDLIVEELNHMKNSYDQIKRRIETGSGRYLTF
                     AIPLMWSHAEYAMALLLKTWRQLQDTGVKQHIL"
BASE COUNT          516 a          404 c          307 g          532 t
ORIGIN      
        1 aacgctgaag gggatcccct atagtgcgaa ttatcttttt ttcaatatcg tccatgcctc
       61 ccacataatc aatgattttg ctggtgttga tatcataggc aaggccattt atggtaaaat
      121 cacggcgttc aacgtcttcc ttaagagttt ttgaaaaaac aactgtatca ggatgcctgc
      181 catcagtgta tttaccatca gccctgtatg tagttatctc atactgcgca tagtgcataa
      241 gaactgtcac cgtgccatgt ttgatacctg ttggcacaac ttttttaaac agcttcatta
      301 catcttctgg atgcgcattg gttgcaaaat cataatcata cacaggtatc cccaatataa
      361 ggtcacgcac agaaccacca attaaataac actcatgccc attgctggtt aatgttgtac
      421 atatggcctt aatttcttta gctactcttg gaggaattgc taatgttacg gtatacgtct
      481 tcataaaatg tgctgcttta ctccagtatc ctgtaattgc cgccatgttt taagcaacag
      541 ggccatggca tactcagcat gcgaccacat gagcgggatt gcaaaggtaa gatagcgtcc
      601 agatcccgtt tcaattcttc tttttatctg atcataagaa tttttcatat gattgagttc
      661 ctcaacaatc aaatcgtagg ttattccgtc aaccataata tcaggtgcaa attccttgtt
      721 cacatcactt ccaggcaacc actccaatgt cataaattca tgaaatctat caggcgtggt
      781 caaatgctca cacaaatagc catcagtgct atagctatct ataatatcca taattgtatc
      841 acctttttca acgttccctg taaaataata atactgtgcc agcattgctg tatactgcac
      901 ccagggacca ttaccagcat gtacatgggt attgggatcc tcaataaacg gcagatagcg
      961 ctgtagcatt cctaaatcag ggtcccatag ggtatcctcg agaaatttca ttgtgttaat
     1021 aaaaatctca tctgccttaa aaatatccgg cactatccct gtaccaaaga aaaacgggct
     1081 cattaatgtt atatcagggc gcaaatcggc ctcaccatca ggtttaagcc ttcgtataag
     1141 cctgtcccca atggtaaaaa cgtttttaac aatggtgcca aatccctctt tcagtacaaa
     1201 agctttttca aatttagttt cacagttaaa ttcttttgcc acctgctcaa ttaaaagcaa
     1261 catgcagtaa tatgcataat taacccatat tgaataacct tcttctattg ctgattcatg
     1321 gatggatgtt gtggaataaa ataaatgtat ttcattacga tagtaccgtt taaccgcaaa
     1381 cattgcccct ttataaattg cctcaatata tgcttcatta tcatgaggaa tatgacccct
     1441 gtttttggct gcaagcagat atctgcacaa aatggataca ccatgcgcca cattgtcttc
     1501 ctgcttgtaa atgcttttgt cagccgcatc aagcccatac cgctgacccc agtatccatc
     1561 gtcacgctgg caattgtaaa taaatttggc aataccggca agaagtctaa atgcaatatc
     1621 ttttgaacgc aacctgctca ttgcaatacg gtacagcatt cgagttgcac aggcactatc
     1681 gcgcggatat acatagggat accgtgaacc tggtaacgtt gcaagcaggg cactcccttt
     1741 tggtgtttca aacgtacat
//
LOCUS       DAOSZP010000494         1758 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_50371_length_1758_cov_2.697005, whole genome shotgun sequence.
ACCESSION   DAOSZP010000494 DAOSZP010000000
VERSION     DAOSZP010000494.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1758)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1758)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1758
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50371_length_1758_cov_2.697005"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            96..>1758
                     /locus_tag="PK348_09705"
     CDS             96..>1758
                     /locus_tag="PK348_09705"
                     /inference="COORDINATES: protein motif:HMM:NF016169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HXK66531.1"
                     /translation="MINLYTSNNLEYLAKQLGENLYQKKKNILSPSTVIVQSLGMQRW
                     ISLKLAQQFGVFLNCRFPFPENFAQEIFKAVTPDYELSPLYTNKSLVWLIVSLLPQCL
                     DEKPFEPVKQYLVVDGTISQVRLYQLAHRLAYMYDEYSVYFYRDILQWEKEPSNAWQV
                     LLWRYIKNNISTDSKICHKAQLLEHVIKALNDPKNTQVLKEKLADEVFLFGITVLPEY
                     YITLFNVVSSIVPVHIFQLNPSKEYWFDIRSEKALLRIQQKIKAKNININDLHFEPGN
                     PLLASLGKVGQEYIGLLLDYNAYDTITDEGQYTIRNSSMLLAHIQNDILHCIERGNGK
                     HEKMAISPDDNSITIHSCHSPLREIEVLRDYIINILNTTAIVPNDIVVMAPDIDVYAP
                     FIKAVFDEQVLHREGLPVLPYAIADQSYKNTSRFISAFSDVLEVFAGRLEADKVCELL
                     DYEEITQRFGIEKHYVMAFHQWMEELNVRWGKDASHRKQYTHTESNAFTWQYAIERLL
                     MGYAVPQGAGLVDDVIPFDCVEGSSAVVLGCFLNFLEALFRFFDEA"
BASE COUNT          540 a          289 c          370 g          559 t
ORIGIN      
        1 aaaaggcagg caatacttga gttaaagaaa ataatacaaa tgcactatac cgatgctgag
       61 ttatttttat ttggatcaaa ggcgagttga gtaaagtgat taatctttat accagtaaca
      121 accttgaata tttagccaag caattaggag aaaatcttta tcaaaaaaag aaaaatattc
      181 tgtccccaag tactgttatt gttcaaagtt tggggatgca acggtggatt tcgctaaagt
      241 tggctcagca gtttggtgtg tttttaaact gcagattccc tttcccggaa aactttgccc
      301 aggaaatttt taaggcagtg actcctgact atgaactatc gccactgtat actaataaat
      361 ctctggtgtg gcttattgtt tcattattgc cacagtgttt agatgaaaag ccatttgaac
      421 cagttaagca gtatcttgta gttgatggca caataagtca ggtgcgatta tatcagcttg
      481 cccacaggct tgcctatatg tatgatgaat atagtgtgta tttttacagg gatattcttc
      541 agtgggaaaa ggaaccatca aatgcctggc aggtgttgct gtggcgatat atcaaaaaca
      601 acatttccac tgattcaaaa atttgccata aggcgcaact tcttgaacat gttattaaag
      661 cgttaaatga tccaaagaat acacaggtgt tgaaggaaaa attagctgat gaggtatttt
      721 tgtttggcat taccgtgttg ccagagtatt acattacact attcaatgtt gtaagcagca
      781 ttgttcctgt tcatattttt cagcttaatc catcaaagga atactggttt gatatacgca
      841 gtgaaaaagc actattgcgt atacaacaaa agattaaggc taaaaatatt aatataaacg
      901 atttgcactt tgagccaggg aatccactgc tggcatcgct gggtaaagtg gggcaggaat
      961 atataggtct gcttcttgat tataatgcgt atgatactat cactgatgaa ggtcaatata
     1021 caattcgcaa tagttcaatg ttactagccc acattcaaaa tgatatatta cactgcattg
     1081 aacgtggaaa tggtaagcat gaaaagatgg caatatcccc tgatgataat tcaataacaa
     1141 ttcattcatg tcattctcca ctgcgtgaga ttgaagttct gcgtgattat atcatcaata
     1201 tattaaacac tactgcaatt gttcctaatg acattgttgt catggcacct gatatagatg
     1261 tgtatgcacc attcattaaa gctgtgtttg atgaacaggt attacacagg gaaggattgc
     1321 ctgtattgcc gtatgctatt gctgaccagt catataaaaa tacaagccgt tttatttcag
     1381 cattttctga tgtacttgaa gtttttgcag gcaggcttga agctgataag gtttgtgagc
     1441 ttcttgacta tgaagaaata actcaaagat ttggaataga aaaacattat gttatggctt
     1501 ttcatcagtg gatggaagaa ctcaatgtac gatgggggaa ggatgcttct caccgcaaac
     1561 aatatacaca cactgaaagt aatgcgttta cctggcagta tgctattgag cgcttactga
     1621 tggggtatgc tgtgccacag ggggcaggcc ttgttgatga tgttatcccc tttgattgtg
     1681 ttgaaggttc atcggctgtt gtgctgggat gttttttaaa ttttcttgaa gcgctattta
     1741 gattttttga tgaagcgc
//
LOCUS       DAOSZP010000495         1758 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_50375_length_1758_cov_2.493247, whole genome shotgun sequence.
ACCESSION   DAOSZP010000495 DAOSZP010000000
VERSION     DAOSZP010000495.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1758)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1758)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1758
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50375_length_1758_cov_2.493247"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(62..868)
                     /gene="fdhD"
                     /locus_tag="PK348_09710"
     CDS             complement(62..868)
                     /gene="fdhD"
                     /locus_tag="PK348_09710"
                     /inference="COORDINATES: protein motif:HMM:TIGR00129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HXK66532.1"
                     /translation="MDIQLETVQNVKAFQYQDSAFEEVTVPISTEYGITIAINGKPFV
                     TIACSGSELVELATGFLVAEGIITSKSEIEGIRFDEKNLTMHVTTKETDAIVQRLFTI
                     RSIVSGCGNASQKFEGLKKLTPPEVSPETIIKIGKQFYQTSELHKLTHGVHSAGLYKT
                     TGECIAFYDEIGRHSAIDKLVGYALYNDIPLHNKIVYSTGRISGEIIQKAMVSGFPII
                     ATKGSPTSLAIQLANEYNVMLIAKVRVNRFSVFTTSIDLVSVFGVGLTLR"
     gene            complement(852..1220)
                     /locus_tag="PK348_09715"
     CDS             complement(852..1220)
                     /locus_tag="PK348_09715"
                     /inference="COORDINATES: protein motif:HMM:NF013738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HXK66533.1"
                     /translation="MKNDILKNHFLHPYNVGIVENYDHKIIVKSDQCNDVITFTVTIT
                     HNTIDDIKFEAYGCGYAIAGASLVSDAVKGKDIDLGIRDVEEMLNTITIPDSNLRCIH
                     LSLKAIKQLKDNLIAHGHTA"
     gene            1596..>1758
                     /gene="ssrA"
                     /locus_tag="PK348_09720"
     tmRNA           1596..>1758
                     /gene="ssrA"
                     /locus_tag="PK348_09720"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT          529 a          374 c          258 g          597 t
ORIGIN      
        1 cttcttgaac agatcctcgt cttaccgcaa ctattttgac ttttttatca ttccttaata
       61 attatctgag cgttaatccc acaccaaata ctgatacaag atcaatcgac gttgtaaaca
      121 cactaaaacg gtttactctc acctttgcaa tcaacattac attgtactca tttgcaagtt
      181 gtattgccag tgatgtaggc gagcctttgg ttgcaataat ggggaatcct gataccatgg
      241 ctttctgaat aatctcaccg gatattcttc cagttgaata cactatttta ttatgaagtg
      301 gaatatcatt atataatgca taccccacca gcttatcaat tgcactgtgc cttcctattt
      361 catcataaaa ggcaatacat tcgccagtag ttttgtacaa acccgcacta tgaacaccat
      421 gggtaagctt gtgtaattct gatgtttgat aaaactgttt cccaattttt ataatggttt
      481 caggtgaaac ttctggtggt gttaattttt ttaacccttc aaatttttgc gatgcattcc
      541 cacagcctga gactatagaa cgaatggtaa aaagacgctg aacaatagca tctgtttctt
      601 ttgttgtaac atgcattgtt aaattttttt catcaaaccg tattccttct atttcacttt
      661 tactggtaat gataccttcg gcaaccagaa accccgtagc aagctccaca agctctgagc
      721 ctgaacacgc aatggtaaca aatggcttac cgttgattgc tatggtaata ccatactctg
      781 tacttatcgg tacagttact tcctcaaatg cgctatcctg atactgaaaa gcttttacat
      841 tttgtaccgt ttcaagctgt atgtccatgt gctattaggt tatcctttaa ttgctttatg
      901 gccttcaatg aaagatgtat gcaccgtaga ttagaatctg gaattgttat tgtgttgagc
      961 atttcttcca catcgcgtat ccccagatca atatctttgc ctttaacagc atcactcacc
     1021 agtgatgcac ctgctatcgc atagccacat ccatacgctt caaattttat atcatcaatt
     1081 gtattatgtg tgatagttac tgtaaaagta atcacatcat tacattgatc acttttgaca
     1141 ataattttat gatcataatt ttccactatc cccacattgt atggatgcaa aaaatggttt
     1201 ttcagaatat catttttcat atttcaaaat tacgcttatt catcatagta caatagaata
     1261 ttctactact tttttcctcc aatatctaat ttaaaataaa acaacatata ccagtcacta
     1321 tcgacataat tattaaaatc ttcattgtgg tttttctata cttgatacct ccctcatata
     1381 aaaaatgaaa taatttacct cacccttaag cacttcttat atgactcact gaattccttc
     1441 caaaccagag cctccaatat tacagccatt gaaaagttct caccaaacca cgtaaaataa
     1501 tagaataatg attttgcatc aaaatatcat atacacaaaa aattatccca ataaatattg
     1561 acatttgtgc atcttattgt atattatata tgcaagggga cgatcttggt ttcgactgca
     1621 atggcgtgtc tacagttgca tgccgagttt ctgctggctc gtaaaaactg gcaggttaaa
     1681 ataagtgcaa acaacgattt agcactcgca gcttaatccc tgcgggcgtc tttatcgatc
     1741 ccgctcctct ggggatga
//
LOCUS       DAOSZP010000496         1756 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_50439_length_1756_cov_2.580835, whole genome shotgun sequence.
ACCESSION   DAOSZP010000496 DAOSZP010000000
VERSION     DAOSZP010000496.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1756
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50439_length_1756_cov_2.580835"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..475
                     /locus_tag="PK348_09725"
     CDS             <1..475
                     /locus_tag="PK348_09725"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HXK66534.1"
                     /translation="ELKKAIYTDRLTGLYNRQYCEELLKENREYSIAVIKPDKFKAIN
                     DTCGHKAGDATLIALAQTIRKASPDAIHIRYRGDEFIVATPPDTDIQSLAQAILKAIG
                     TINIASITSGLITSITASIGTGSGVFCQRLIDSVYERMWSVYHKGGNSIAVEGDM"
     gene            472..1422
                     /locus_tag="PK348_09730"
     CDS             472..1422
                     /locus_tag="PK348_09730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HXK66535.1"
                     /translation="MNGLDVLLSKVTIFKKLTQEEILQLVPFLSQKDYPEGSIIFDEG
                     TYGSELFIVASGEVAAFIKLPDGSLREVARFTTGDFFGDMAIFENDVRSATCIAQTDC
                     RLLSLTASNFFTIINSHPSAAIKIMYDMLTVISQRLMKTGSFLTEMIQWGEAARKRSI
                     IDEFTGIYNRSYFEDTITLKMTQEKPFCLIMVDLDYFRKINDQYSHDVGDRAILAIVD
                     VLKNTFRENDALCRYGGDEFAVIFDGNDVAHAHKLAEKARQNVNAIDCRHLGMDNFKL
                     SLSMGIAAFPTFDRDSIRAKADEALYSAKERGRNCVVIAQ"
     gene            complement(1427..>1756)
                     /locus_tag="PK348_09735"
     CDS             complement(1427..>1756)
                     /locus_tag="PK348_09735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66536.1"
                     /translation="PQYLGVYHINASGHEGILVLQVKDGVLYGSVRFPNWANGVWEPL
                     KGVNIKNNTLWFTRSVTNEQERIKTGAREYFTQQYVGTYKDNGKKIIGFYKLRGAKYL
                     FEAYRKK"
BASE COUNT          535 a          361 c          357 g          503 t
ORIGIN      
        1 cgaacttaaa aaagccatat acactgacag gcttacaggc ctttacaaca gacagtattg
       61 tgaagaactt ttaaaagaaa acagggaata ctcaattgcc gttattaaac ctgataaatt
      121 caaagccatc aacgatacct gcggacataa agctggtgat gccaccctca ttgctctggc
      181 acagacgata cgcaaagctt cccccgatgc cattcacatc cggtacaggg gcgatgaatt
      241 tattgttgcc actccccctg atacagatat tcaatcactg gcacaagcaa tactgaaagc
      301 cattgggaca ataaatattg catcaatcac cagcggactg attacatcaa taacggcaag
      361 tattggcact ggaagtggag tgttttgcca gaggcttata gacagtgtgt atgagcgcat
      421 gtggtcagta taccataaag gtggaaattc aatagcagtg gaaggtgaca tgtgaacggg
      481 cttgatgtat tgctttcaaa agttactatt ttcaaaaaac taacgcagga agaaatttta
      541 cagctagtac cgtttctttc acaaaaagat tatcctgaag gcagtatcat ctttgatgaa
      601 ggaacctatg gtagtgagct tttcattgtt gccagcggcg aggttgctgc gtttatcaaa
      661 ttaccagatg gtagtcttcg cgaagttgcc cgctttacca caggcgattt ctttggcgat
      721 atggcaatat ttgaaaatga tgtgcgctct gcaacctgta ttgcacaaac cgattgcagg
      781 ctattgtcat tgacagcttc caactttttc acaatcataa atagccatcc atctgctgct
      841 atcaaaatta tgtatgatat gctcacggtt atatcgcagc gcctcatgaa aaccggctca
      901 tttttaaccg agatgattca gtggggcgaa gcagcacgta agcgttcaat tattgatgaa
      961 tttacaggta tatataaccg cagttatttt gaagatacta ttacattaaa aatgacccag
     1021 gaaaagccat tttgtcttat catggtggat cttgattatt tcagaaaaat aaatgatcag
     1081 tatagccatg acgttggcga cagggcaata cttgctatag ttgacgtact aaaaaataca
     1141 ttcagagaaa atgatgctct atgccgttat ggtggtgatg agtttgcggt aatttttgat
     1201 ggcaatgatg tggcacacgc acacaaactt gccgagaaag cacggcaaaa cgttaatgca
     1261 atagattgcc gccatttagg catggacaat ttcaaactat ccttgagtat gggcattgct
     1321 gcattcccca catttgacag ggatagtatc cgtgcaaagg ctgatgaggc attatatagt
     1381 gcaaaagaac gtggtcgtaa ttgcgttgtg attgcacaat gataatttat ttttttctat
     1441 acgcctcaaa taaatatttg gcaccacgta atttataaaa accaataatt tttttgccat
     1501 tatccttata ggtaccaaca tactgctggg taaaatattc acgggcacct gtctttatcc
     1561 gttcctgttc attggtcacc gaacgtgtaa accacagggt attatttttt atattcactc
     1621 cttttaatgg ttcccacaca ccatttgccc agttgggaaa acgcaccgaa ccatacaaca
     1681 ctccgtcctt tacctgaagc accagtatac cttcatgtcc tgaagcattg atgtggtata
     1741 cacccagata ctgtgg
//
LOCUS       DAOSZP010000497         1754 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_50493_length_1754_cov_3.816363, whole genome shotgun sequence.
ACCESSION   DAOSZP010000497 DAOSZP010000000
VERSION     DAOSZP010000497.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1754)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1754)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1754
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50493_length_1754_cov_3.816363"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..812
                     /locus_tag="PK348_09740"
     CDS             <1..812
                     /locus_tag="PK348_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003868969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HXK66537.1"
                     /translation="NSELTKALLKAKVIGIAYETIQLDDGTLPLLTPMSEVAGRMSIQ
                     VGEFFLQKENGGRGVLLSGIPGVEPGNVVILGGGVAGTNAAKMAIGTGANVYIVDVNL
                     KRLRYLDDILGGRIKSVVSNRYNIEKLISIADLVVGAVLIPGAKTPKLITKEMLSLMK
                     EGSVIVDVAVDQGGCVETTKPTYHDNPTFIVDGVIHYCVANMPGAVPRTSTFALANVT
                     LPYALKIANMGYKEALKSDPSLMKGLNILHDKLVCKPVADAQGIECCPIEL"
BASE COUNT          568 a          282 c          367 g          537 t
ORIGIN      
        1 ctaattctga attaactaaa gccctcctaa aagcaaaggt cattggtatt gcatatgaaa
       61 caatacagct tgatgatgga acactgccgc ttttgacccc catgagtgaa gtagcaggaa
      121 gaatgtccat tcaggtgggt gaattttttt tacagaaaga aaatggaggt aggggtgttt
      181 tattaagtgg catcccaggg gttgaaccag gaaatgtagt tatcttaggt ggtggcgttg
      241 caggcacgaa cgcagcaaag atggcaattg gcacaggggc aaatgtatat atcgttgatg
      301 ttaatttaaa aagactaagg tatcttgatg atattttggg aggtagaatc aaatcagtgg
      361 tgtcaaatag atataatatt gaaaagctta tatccatagc agatttagta gtaggcgctg
      421 ttcttatacc aggggcaaag acgcctaaac ttattacaaa agagatgctt tcactgatga
      481 aagaagggtc agttattgta gatgtggcag tagaccaggg aggttgcgtg gagacaacaa
      541 aacctacata tcacgataat cctaccttta ttgtagatgg ggttatacat tactgcgttg
      601 caaatatgcc tggggcagtt ccaaggacat ccacatttgc tttagctaac gttacccttc
      661 cctatgcact aaaaatagca aacatgggtt acaaagaggc attaaaatct gatccttctc
      721 ttatgaaagg gcttaatatt ttgcatgata aactggtgtg caaaccagtt gctgatgccc
      781 agggcattga atgctgcccc attgagctgt gaaaacactt tttatacaac cacctattga
      841 agatttttat gatacgggca taaggacata cccgttgggg ctgctctatc ttgcagagaa
      901 ggtgaaatcc ttatgcaatg taaaaattat tgattttaga acaaatgcaa aaccccaatc
      961 agcaaaaaca aatccttttc ctgaactgtc tgtttactct aaggaatcgc tatttagccc
     1021 tttttctcta tttaggaaat acagtaggtt cggctcatcc caaggacaac gctttttacc
     1081 gtaatgaagc ttgcaaggtt tgtaaatttt ttaaagggac ttgccgatag gtatggctca
     1141 atgcatgttc agatataccc agacttcctt ttggccaacc aaacagcgat ataataaaag
     1201 agatactttc acggttaatc aatgaaaata ggtttgtggc atatgataga gaaaaaaagg
     1261 actttgtcta tgaaaattgc gatgaccagg tggtgaaata cttttttgaa cacacaaaaa
     1321 aaggatttat caaagactac aaaacccatg aaattattac ctttggttag ttatggcgat
     1381 ttttggtttt gattattttc tttggtcgaa cgggttattt tatgtgtttt ctggaaagat
     1441 ctttttttca aaggcatgct ttatccttaa ggtcaagcat ttcgatgatt gataaaaaaa
     1501 gtaacaattt aaataaaaga catgtccata tattgtttga cattttttat ccatatactt
     1561 tataatcctt tttgttatga aaaatttaaa gtttttgata tgtcttttgc tttcggtttt
     1621 tttgctgtgt agttgcagct caacctcttc aaagaagaaa gaaaaggata ctactgctaa
     1681 acaacaaata gaaaaggtag aggagtcaga gcctaaacca ggggatataa aggtaataga
     1741 tggggtggaa tata
//
LOCUS       DAOSZP010000498         1753 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_50548_length_1753_cov_2.726737, whole genome shotgun sequence.
ACCESSION   DAOSZP010000498 DAOSZP010000000
VERSION     DAOSZP010000498.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1753
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50548_length_1753_cov_2.726737"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..897
                     /locus_tag="PK348_09745"
     CDS             <1..897
                     /locus_tag="PK348_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012176515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXK66538.1"
                     /translation="AGLGNAAILAVANDEQLERFGNKWAAMAITEPGAGSDSGAITTT
                     ATRDGDYWILNGEKIFVTCADRSEVVVVWATVDKKAGKAGIKSFVVEKGTPGFTLEHL
                     EHKLGIRASDTGTFVLSDCRIPYNNILGDPEVKMSTEGFKGVMKTFDNTRPLVASMAT
                     GIARAAVEFTREQYEKKGYAFDYKKNLSNVSAVEREWYLMDANVEAMRLLTWRAAWMA
                     DNNQFNNLEASMAKAKAGRYATLITQRCSELLGPVGYSTQYLAEKWMRDSKIMDIFEG
                     TGQIQHLIIARNVLEMSSKELK"
     gene            924..>1753
                     /locus_tag="PK348_09750"
     CDS             924..>1753
                     /locus_tag="PK348_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012176514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HXK66539.1"
                     /translation="MQSINDVKKIAVIGSGAMGHGIAQVCAQNGLMVTMIDIKQEFLD
                     NALKKVKESLDFLASKGKLQGTVDEILGKISVTTDLQKGVSDAQVIIEAVPENMQLKK
                     DVFAKASSACASDAILATNTSTMSITEIATAVTNPERFAGMHFFNPVTRMKLVELIYG
                     AKTSENAINILFELTKKIDKFPVKVLKDRPGFIVNRISAPNQALLSAILDEGKIHPAQ
                     IDGKMKKMGVKMAPFETADFVGLDVFCHTLEYYAQTLSPQYKPGKFLLGCLDKGHLGM
                     "
BASE COUNT          579 a          260 c          419 g          495 t
ORIGIN      
        1 gctggtttag ggaatgcagc aattttggca gttgccaatg atgaacagtt ggaacgattt
       61 ggcaataagt gggctgctat ggcaattacg gagccgggtg caggttctga ttcaggagct
      121 atcacaacaa cagctactcg tgatggtgat tactggatac tcaatggaga aaagattttt
      181 gtaacctgcg cagacagaag tgaagtggtt gtggtatggg cgacagttga taaaaaagct
      241 ggcaaagctg gtataaaatc atttgtcgtg gaaaaaggaa cacctggttt tactctggag
      301 catttagaac ataaattagg catcagggcc tctgatactg gaacatttgt actcagcgat
      361 tgtagaattc cctataataa tatattgggt gaccctgaag tgaaaatgtc cactgaggga
      421 ttcaaaggtg ttatgaaaac atttgataat accagaccat tggttgcttc aatggccact
      481 ggtattgcac gtgctgctgt agaatttacc cgggaacaat atgagaagaa aggatatgca
      541 tttgattata agaagaattt atcaaatgtt tctgcagttg agcgtgagtg gtatttaatg
      601 gatgccaatg ttgaagctat gagactttta acctggcgtg ctgcctggat ggctgataat
      661 aaccagttta ataatttaga agcatcaatg gcaaaagcta aggcgggtag gtatgcaaca
      721 ttgataaccc agcgatgcag tgaattgctt gggccggtag gatattcaac gcaatatcta
      781 gcagaaaaat ggatgaggga tagcaagatc atggatattt ttgaagggac tggacaaata
      841 cagcatttaa ttattgctcg aaatgtactg gaaatgtcaa gtaaggaact taaataacat
      901 gatttttata aatggaggta attatgcaga gtattaatga tgtaaagaaa attgcagtta
      961 taggttcggg ggcaatgggt catgggatag cacaggtttg tgctcagaat ggtttaatgg
     1021 ttaccatgat agatatcaag caggaattcc ttgataatgc gttaaagaaa gtaaaggaaa
     1081 gtttggattt ccttgcatca aaaggaaaac tgcagggtac tgttgatgaa attcttggga
     1141 aaataagtgt tacaacagat ttacaaaaag gagtatcaga tgctcaggtg atcattgagg
     1201 ctgtccctga aaatatgcaa ctgaagaagg atgtatttgc aaaagcttca tctgcatgtg
     1261 cttcagatgc aatactggca acaaacacat caacaatgag cattactgaa atagctactg
     1321 ctgttacaaa tcctgagcga tttgctggga tgcatttttt caaccctgta acaagaatga
     1381 aattggtgga gttaatttat ggcgcaaaaa catcggaaaa tgctataaat attttgtttg
     1441 aattaacaaa gaaaattgat aaattccccg taaaagtttt gaaagataga cctggtttta
     1501 ttgttaacag aatcagtgca cctaaccagg cattactcag tgcaatttta gatgaaggca
     1561 aaatccatcc tgcacaaata gatgggaaaa tgaagaagat gggtgttaaa atggctcctt
     1621 ttgaaacagc tgattttgtg gggcttgatg ttttttgcca tacactggag tattatgcac
     1681 agacactatc gccacaatat aaacctggaa aatttttact tggatgcctg gataaaggtc
     1741 atcttggcat gaa
//
LOCUS       DAOSZP010000499         1748 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_50734_length_1748_cov_4.423509, whole genome shotgun sequence.
ACCESSION   DAOSZP010000499 DAOSZP010000000
VERSION     DAOSZP010000499.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1748)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1748)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1748
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50734_length_1748_cov_4.423509"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="PK348_09755"
     CDS             complement(<1..314)
                     /locus_tag="PK348_09755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66540.1"
                     /translation="MRNFASILTFVIAIVVFASSCATLNTQDTKLVDQLKAENRVCSQ
                     NLNLFMRENDVLKAENAKYKKENVNLQSRINLLQSDLNALQTKYKNDIAMWNQRYDSL
                     QQ"
     gene            667..1656
                     /locus_tag="PK348_09760"
     CDS             667..1656
                     /locus_tag="PK348_09760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66541.1"
                     /translation="MKFLTKVCFIVLVLFASTTVFAAWVYVPMSINAQKGDIVLSTSP
                     GFIMDLLAILGCYWSHSGMAVDNGYNIRHNTMYVSQIPIEYNYIWFIKTTPKRLDPTR
                     LSNGLPGILTEDIDTAYNTTLNFHAAGGAVLKPTVANEALYRQYLNAAADVFNYLKAY
                     YRVHAYVNMYQLDYANYYITGRGNHCSGTCWYANYFAGKTMAVATIPPALVTQCANSL
                     YTSVKNMVRDEAGGFGAFIIDIEGLFGTGADEKIANQIVNTFGFDRSTDTSSYWRNYI
                     NQVTATANAPDHLLLSSYTNPSGHNVGVQTTSTSYYGQVEPLVITDGYYIEVP"
BASE COUNT          532 a          342 c          319 g          555 t
ORIGIN      
        1 ttttgctgta gtgaatcata ccgctgattc cacattgcaa tatcattttt atattttgtc
       61 tgcaacgcat tgaggtctga ctgcaacaga tttatccttg attgcagatt tacgttctcc
      121 tttttatatt ttgcgttttc agctttcagc acatcatttt cacgcatgaa gaggttcagg
      181 ttctgcgaac aaaccctgtt ttcagctttc agttgatcca caagttttgt atcctgggta
      241 ttgagcgtag cgcatgaaga tgcaaaaaca acaatagcaa tgacaaaggt taaaatgctt
      301 gcaaaatttc tcattaatag cccccacctg cctgataaat atagtatgta taccctaccg
      361 gttaaaaaaa gaattttact tagcataagt aatacagtaa tcttaagcaa actttagtga
      421 gattcaatct ttttttgtta ctgtataaaa aattattcca aataaaagga tttttaaggc
      481 tctgacccca aaattaaaaa tcttaataaa ataccgacta tatggtattt ttttagttga
      541 caatttttag cttatagcat tagatacagc aaattacacc gcactgagtg aataatcact
      601 cattttgcaa acgatcctat ttcctatctc acggtgaata acacttaaat gttatatggg
      661 ggttctatga aatttttaac aaaggtttgt tttattgtgc ttgtgctctt tgccagcaca
      721 acagtatttg cagcatgggt ttatgtgccc atgagcatta atgctcaaaa aggcgatatt
      781 gtcctttcaa ccagccccgg gtttattatg gacctgcttg ctattctggg ttgttactgg
      841 agccatagcg gaatggctgt tgataatgga tataatatcc gccataatac catgtatgtc
      901 agccagatac cgatagagta taactacatc tggtttatca aaacaacacc aaaacgcctt
      961 gatccaacaa gattaagcaa tgggcttcct ggtattctga ctgaagatat tgataccgcg
     1021 tataatacaa cgctgaattt ccatgctgcg ggtggcgctg tacttaaacc cactgttgca
     1081 aatgaagcat tgtacaggca atacctgaat gctgctgcag atgttttcaa ttacctcaaa
     1141 gcttactacc gtgtacatgc atatgtcaat atgtatcagc ttgactatgc caactactac
     1201 attactggcc gtggcaacca ttgttcgggt acatgctggt atgctaacta ttttgccggc
     1261 aagacaatgg ctgtagctac aataccacca gcacttgtta cacaatgtgc caattcactc
     1321 tatacatcag ttaaaaatat ggttcgtgat gaggctggag gatttggtgc atttatcatt
     1381 gatatagaag gtttatttgg tacaggagcc gatgaaaaaa tagccaacca gattgtcaat
     1441 acgtttggtt ttgacaggtc aaccgataca tcttcatact ggagaaatta tattaatcag
     1501 gtaacagcta cagctaatgc ccctgaccat ttattactgt catcatatac aaatccatca
     1561 gggcataatg taggtgtaca gacaacttct acctcatact atggccaggt tgaaccgctt
     1621 gtcattacag atggctatta cattgaagtg ccttaatagt gatttatcca taagtacaat
     1681 taaaaggggg tgccccaaaa gaggtcgttg agtaaatttt gcaaacatta tatcgtaata
     1741 ctcgctca
//
LOCUS       DAOSZP010000500         1746 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_50805_length_1746_cov_3.262567, whole genome shotgun sequence.
ACCESSION   DAOSZP010000500 DAOSZP010000000
VERSION     DAOSZP010000500.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1746
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50805_length_1746_cov_3.262567"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1576)
                     /locus_tag="PK348_09765"
     CDS             complement(<1..1576)
                     /locus_tag="PK348_09765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66542.1"
                     /translation="MDYEIYDVNNILLPVEHKIGALNRAKNLVAEMTHPNIDWKYMVT
                     ELRAYLYDYMYDIVPHSDKVLPVIFHYLKEATVRKRGSTLRAADTFLDRYLFLIKKEI
                     EGDSSLENVTAMFDNESIHFCQILIADTADGFYLEDVNLRILQLLELSLKRKKVDETL
                     FELCTEIIINQFKLYVERSIIVDDEEVYSLQNLWSIEHEHILKLEQLVKSVTKKAYQE
                     KLIKANALKNSKKDRATLLAEIKELIDFHHNTTSWEKICIAAKECIAQNVIEYDDVVL
                     ALLTFLVKKSQEGRDANLQLYISRSVASLCSVMVQQQRFVLLRQVVQMVVPVLVAEIE
                     RGGNYNAAFATILNIGKTVVQSDNRQIIDLFVDILVHAKFCFPQFTGIAQDWSVMVNA
                     SHLANIRTWLELIELNPVYMKRLAASLIANLTLGGVFLKDTDVFQRDISRLLNSNYKD
                     VFYLIISLAAVFPAFYHDIGATGNIRAFTERVDTNHQMNDLIHFVRKQVHVESSSRTV
                     VLLQRVMDFWLTGDKEL"
BASE COUNT          521 a          376 c          327 g          522 t
ORIGIN      
        1 atagctcttt gtcgccggta agccagaagt ccatcacgcg ctgtaacagc accaccgtac
       61 ggctgcttga ttccacatgc acctgcttgc gcacaaaatg tatgaggtca ttcatctggt
      121 gattggtgtc aacacgctcg gtaaacgcac ggatgttgcc ggtagcacca atatcatggt
      181 agaatgcagg gaacactgcc gcaagcgata tgataaggta aaatacatct ttatagttgc
      241 tgtttaaaag gcgtgaaata tcacgctgaa atacatccgt atcttttaaa aagacacccc
      301 ccaacgttaa attggctata aggcttgcag ccaggcgctt catatacacc gggttaagct
      361 caataagctc aagccacgta cggatatttg caaggtggct tgcattgacc ataaccgacc
      421 aatcctgagc aatgccggta aattgtggaa agcagaattt tgcatgtacc agaatatcga
      481 caaaaaggtc aataatctgc cggttatcgg attgaacaac cgttttgcca atattcagga
      541 tagttgcaaa cgcagcatta taattgccac cacgctcaat ctcggcaaca agcacaggta
      601 caaccatctg aacaacctgt cgtagtagca caaaacgttg ctgctgtacc atcacgctgc
      661 ataacgatgc aacacttcgt gatatataca gctgaagatt tgcatccctg ccctcctgcg
      721 attttttaac cagaaacgtt aagagtgcca gcaccacatc atcatattct atgacattct
      781 gagcaatgca ctcttttgca gcaatacaga tcttttccca tgaggtggta ttgtggtgaa
      841 aatcaatcaa ctcttttatt tcagcaagga gtgttgccct gtctttttta ctgtttttta
      901 aagcattagc ttttattaat ttttcctgat acgctttttt agtaactgat ttaacaagct
      961 gctcaagctt aagtatgtgt tcatgctcaa tgctccacag gttttgcagt gaatacactt
     1021 cttcatcgtc aactatgata ctgcgttcaa catacaattt aaactggttt attataattt
     1081 cagtgcacag ttcaaaaagg gtttcatcta ctttttttct tttaagcgat agttctaata
     1141 attgcaaaat cctgagatta acatcttcca ggtaaaaccc atcagcagtg tcggcaatta
     1201 atatctggca aaaatgtatt gactcattgt caaacatggc agtgacattt tccagggaac
     1261 tatcgccctc aatttctttt ttaattaaaa agagatagcg atccagaaag gtatccgcag
     1321 cacgcagcgt tgaaccacgt tttcgtaccg ttgcttcttt caggtaatga aagataaccg
     1381 gcaaaacttt atcactgtga ggaactatat catacatgta atcatacagg taggctctga
     1441 gttcggtgac catgtacttc cagtcaatat tgggatgcgt catctcggca accagattct
     1501 ttgcacggtt taaggcacca atcttatgct ccaccggaag cagaatattg ttcacatcat
     1561 agatttcata gtccatggca tcaccttgta gtgtcagtag attgctctta ctactgttga
     1621 aaatatacta tgctttagaa tgatgtcaat tattttaaaa tttgtaaaat ttgcttgaca
     1681 taaatattat acataacaca tacttaaatt aatgtggtgt actcataaag tggattgaac
     1741 cagtgc
//
LOCUS       DAOSZP010000501         1745 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_50849_length_1745_cov_2.978107, whole genome shotgun sequence.
ACCESSION   DAOSZP010000501 DAOSZP010000000
VERSION     DAOSZP010000501.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1745
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50849_length_1745_cov_2.978107"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            59..610
                     /locus_tag="PK348_09770"
     CDS             59..610
                     /locus_tag="PK348_09770"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HXK66543.1"
                     /translation="MQNFDFHSNNKNFYTDYNAQDYREYTIPQNYATPRSFSQKKAHR
                     MLTIIIALIIMSFTAGLVVGIKFAGGQSKPIIDPHTKIALNNAAQKAKTLVEPRQQPQ
                     FPKSEYPYAIKVGNAYTRDEATTLAQILSKTGQRVIIAKNKDQHNVYAGPYTSLQDAK
                     KSLKIIMEQSKNGNFKNAIILKR"
     gene            620..1093
                     /locus_tag="PK348_09775"
     CDS             620..1093
                     /locus_tag="PK348_09775"
                     /inference="COORDINATES: protein motif:HMM:NF005840.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HXK66544.1"
                     /translation="MKITIRKATSDDAEQILALLSPYVKKGILLPRSKEEIEKTSDSF
                     TVAIAQKKIIGVVSQYKYNTKLFEIRSLAVNSRHHHKGIGKQLVEHAVNDILQQHRDA
                     KIFCLTYNPSFFKKAGFMAVAKETLPDKIWKDCQYCQHRDNCTETAMVYSLRSRG"
     gene            complement(1082..>1745)
                     /locus_tag="PK348_09780"
     CDS             complement(1082..>1745)
                     /locus_tag="PK348_09780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66545.1"
                     /translation="YTSKVKSLWKAGHRKQSLVIAKSILALKESSMNIVKAKKDIIAF
                     MRDIQMKHGKRFVDINRDAWLYLTREDTTMYIFNDKVPYIVALQGNSEIIAAKSVDSL
                     QYSSTVMRAGIRFSDTSGYDAIMTIVAESFAGTIESIRVLEQHWRTIAFDTFDRELLK
                     YSDSIALYSFVTRDNVELKGMELLLAKENRGYCVRIFTTKERYDLYKETMLTIINSFS
                     TP"
BASE COUNT          600 a          344 c          291 g          510 t
ORIGIN      
        1 tcaatatatt catagtacta cacatcataa aattttatca taaaaggtct gatagaatat
       61 gcagaatttt gatttccatt caaataataa aaacttctat actgattaca atgcacagga
      121 ttatcgcgaa tatacaatac cacaaaacta tgcaacaccc agatcttttt cacagaaaaa
      181 agctcataga atgctgacaa ttattattgc tttgataata atgtctttta ctgcagggct
      241 tgttgttggc attaaatttg caggaggtca gagtaaacca attatagacc ctcatacaaa
      301 aatagcattg aacaatgctg ctcaaaaggc aaaaacactg gttgaaccca gacagcagcc
      361 acaatttcca aaatcagaat acccgtatgc catcaaggtt ggcaatgctt ataccaggga
      421 tgaagccacc acattagctc aaatattaag taaaacaggg caacgtgtta tcattgcaaa
      481 aaacaaagat cagcataatg tatatgcagg cccctacacc tcactgcagg atgcaaaaaa
      541 atcattgaaa ataatcatgg aacaatctaa aaatggcaac ttcaaaaacg ctattattct
      601 aaaacgctga ttttttataa tgaagattac tatccgaaaa gctaccagtg atgatgcaga
      661 acaaattctt gccttgcttt ccccgtatgt aaaaaagggc atactgcttc cacgaagtaa
      721 agaggaaatt gaaaaaacca gcgactcatt tacagttgct atcgcccaaa aaaaaattat
      781 tggtgttgtt tcacagtata aatataatac taagcttttt gagataaggt cacttgcagt
      841 taacagcagg catcaccata aaggtattgg aaaacaactt gtagaacatg cagtaaatga
      901 tatattgcag caacacaggg atgcaaaaat tttttgcctt acctataatc cttctttctt
      961 taaaaaagca ggatttatgg cagttgccaa agaaacactc cccgacaaaa tatggaagga
     1021 ttgccagtac tgtcagcaca gggataattg tactgaaacg gcaatggtgt attcgctccg
     1081 atcaaggggt tgaaaagcta ttgataattg taagcatggt ttctttgtaa agatcatacc
     1141 gctcttttgt ggtaaaaatc ctcacacaat atcccctgtt ttcttttgca agcaaaagtt
     1201 ccataccttt gagttctaca ttgtcccgtg taacaaatga atacagcgca atactgtcac
     1261 tgtatttcaa taattcacga tcaaatgtgt caaatgcaat agtacgccag tgctgctcca
     1321 atacacgtat ggattcaata gtaccagcaa agctttcggc tactatcgtc atgatggcat
     1381 catatccact ggtatcagag aaccgtatgc ctgctctcat cacggtggaa gaatattgca
     1441 acgaatcaac tgattttgca gcaataattt ctgaatttcc ctgcaatgcc actatatacg
     1501 gaactttgtc attgaatata tacattgtag tatcttccct ggttaaatat aaccatgcat
     1561 cccgattaat gtcaacaaaa cgttttccat gcttcatctg tatatcacgc ataaatgcaa
     1621 ttatgtcttt tttggctttt actatattca tggatgattc tttcagtgcc aaaattgatt
     1681 ttgcaatgac aagtgactgt ttacggtgcc cggctttcca caaagatttt acttttgagg
     1741 tatag
//
LOCUS       DAOSZP010000502         1743 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_50902_length_1743_cov_3.936019, whole genome shotgun sequence.
ACCESSION   DAOSZP010000502 DAOSZP010000000
VERSION     DAOSZP010000502.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1743
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50902_length_1743_cov_3.936019"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            212..>1743
                     /locus_tag="PK348_09785"
     CDS             212..>1743
                     /locus_tag="PK348_09785"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HXK66546.1"
                     /translation="MKKWFNIVLTYPKTVIVIIIVITVLLGSGVFKIQFDSSLDAVMP
                     RHDPEYLLNEEVKKTYGNTGKFIIIDVASSDMLQPHILTLVSNLHDDIDEYRRFDEGR
                     EMHRLSLLQELSHNKNITKQMLLQTFADDPVFTRYIMQTLQQLDIEGDRLSQRDMTKI
                     IKKFTRTIEVKRNETVDLIISPFTMKDLHGQNDTLYVRDIVEKDETGKRKIPQTAEEI
                     AAFKKRLTNNPAFEKGIYVKDAQGTITDFGILLRLVDQPQYDPLVKEILDIISYYQGT
                     VDIVIQGVPVTHHEINEYMKRDLERFIPLVLLAVLIVFYLNFRSVRGMLLPFATLVFA
                     DLWVIGLMGHLGFKLNVIGVALPPLMTAVGSSYSIHILNRYYIDYPAISRLGKYKGIM
                     SSSMHISLTVILAALTTALGFFTLITNQVSSIREMGVFAAIGVIFSVIIALSMIPAVL
                     MLLPHKEKKLQFSSKVFAYLSNLSTVDTIVTTLAHWAVKKTPVVMSILFILICIAFFG
                     LS"
BASE COUNT          546 a          332 c          326 g          539 t
ORIGIN      
        1 gtactggata gcaacggtaa cggaaaacca gatagtgggg aatatattgg ttactattgg
       61 aagagagata ttacgacact atggtcatat gtaccaataa aactaccttt acctttgggt
      121 gatggtactt cgattttgaa caagacagtg cgtttttcaa gttatacata ttaagttgca
      181 caatcaataa taacaataaa ggaatcggct tatgaaaaag tggtttaaca tagtgcttac
      241 ctaccccaaa acagtgatag taattatcat cgtcatcacc gtactattag gcagtggcgt
      301 atttaagatt cagtttgata gctctcttga tgctgtcatg cccagacatg atcctgaata
      361 tttactgaat gaagaagtaa aaaagaccta tggcaatacc ggcaaattta tcatcataga
      421 tgttgcatca agtgacatgc tgcaaccaca cattttaaca ctggtatcca atcttcacga
      481 tgatatagat gaataccggc gatttgatga aggcagggaa atgcaccggc tttctcttct
      541 acaggaacta tcgcacaaca aaaacattac taaacaaatg ctgttacaga catttgcaga
      601 tgatcctgtt tttacacgct acattatgca aaccttacaa cagcttgaca ttgaaggtga
      661 caggctttca caacgcgata tgacaaagat tattaaaaaa tttaccagaa ccattgaagt
      721 caaacgcaat gaaaccgttg atttaatcat ttcaccgttt accatgaaag accttcacgg
      781 ccaaaacgat accctgtatg tgcgcgatat agtggaaaaa gatgaaacag gcaaacgaaa
      841 aatcccccaa actgctgaag aaattgctgc atttaaaaag cggctcacca ataaccctgc
      901 atttgaaaaa ggcatttacg ttaaggatgc tcaaggaaca atcactgatt ttgggatact
      961 gcttcgtctt gttgaccagc cacaatatga tcctttagta aaagagattc tggatatcat
     1021 cagttactat cagggtactg ttgacatagt tatacagggt gtccccgtaa cccatcatga
     1081 aattaatgaa tatatgaaaa gagatcttga acgctttata ccactggtac ttttagcagt
     1141 gctgattgta ttttatttaa attttagatc agtacgggga atgcttcttc catttgccac
     1201 actggtattt gctgatctgt gggttattgg actcatgggg catctgggat tcaagcttaa
     1261 tgtgataggc gttgcattgc caccacttat gacagctgtt ggcagttcat attctattca
     1321 catccttaac cggtattata ttgattaccc ggcaatttcc cgtttaggga aatataaagg
     1381 aataatgtct tcatcaatgc atatctcact tacggttatc cttgctgctt taactactgc
     1441 tttaggtttt tttaccctca taaccaatca ggtttcatcc atcagagaga tgggagtatt
     1501 tgcagcaata ggagttattt tttctgttat tattgcactc tcgatgattc ctgcagtatt
     1561 gatgctgctt cctcataaag agaaaaagct gcaattttca tcaaaggttt ttgcatacct
     1621 tagtaattta tcaacagttg atacaattgt tactaccctt gcacactggg ctgtgaaaaa
     1681 gactcccgtt gtcatgagca ttttatttat tctgatatgt attgcatttt ttggcttaag
     1741 taa
//
LOCUS       DAOSZP010000503         1743 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_50903_length_1743_cov_3.654028, whole genome shotgun sequence.
ACCESSION   DAOSZP010000503 DAOSZP010000000
VERSION     DAOSZP010000503.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1743
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50903_length_1743_cov_3.654028"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            146..1006
                     /gene="purU"
                     /locus_tag="PK348_09790"
     CDS             146..1006
                     /gene="purU"
                     /locus_tag="PK348_09790"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016777871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HXK66547.1"
                     /translation="MHSKESAILLISCPDKKGIVYKISHFIYSNNGNILRSDQHADEE
                     SGTFFMRIEWDLDGFAIEKNKIIKAFEPIAAEYSMNWQLHFSAKQQRTSIFVSKMDHC
                     LYDILLKAKEGEIATDIACIISNHESLGTIAKYFNIPFFVFQKTKENKLQQEQKELAV
                     LKDFDVDCIVLARYMQILSAHFVSHYPNKIINIHHSFLPAFKGAKPYHQAYQHGVKII
                     GATSHYVTELLDQGPIIEQDVIRVSHKDSIEDIIRKGKDIEKLVLSRALKKHLEHKVL
                     VYANKTIVFD"
     gene            complement(1010..>1743)
                     /locus_tag="PK348_09795"
     CDS             complement(1010..>1743)
                     /locus_tag="PK348_09795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66548.1"
                     /translation="ANLLAKIYLDHYNDRVRALQYFVESLKINDNQPDIHLEVGKLYY
                     FFSEYSKAIEHLQRTIAQKNDVYAHYYLVLIYNLQKNYESAASHIVVCNAITLQQTKP
                     EIEKAQAAQKDNNTNVAMAHYKKILEINPVNREAYVQLSHYYRIQKKIDMAIQILEDC
                     KKVYPKDTDVLLTLAHMYFEFKHPKRREYFIKQAIDLCKVAIGIDSSHCEAYSLLFEI
                     YKELGEVPLRDEHAAKYDACLGGSR"
BASE COUNT          576 a          302 c          300 g          565 t
ORIGIN      
        1 tgaggagtat tatcggaaaa aatattcgta tgtagtggat tgggtgtaaa aaaaataaga
       61 aaaggggtca gagcataata attcattgac aataaataaa caacagcaca tctattaata
      121 aataaacact caatggggtt ttcctgtgca ttctaaagaa agtgctattt tacttatctc
      181 ttgtcctgat aaaaagggga tcgtttataa aatatcacat tttatatatt ccaataatgg
      241 taatatttta cgatctgacc agcatgcaga tgaagagtcg ggaacttttt tcatgcgtat
      301 tgaatgggac ttggatggtt ttgccataga aaaaaacaag attataaaag cctttgagcc
      361 aattgctgct gaatacagca tgaactggca attacatttc tcagcaaaac agcaacgtac
      421 atcaatcttt gtttcaaaaa tggaccattg cctgtatgat attctcctta aggcaaaaga
      481 aggcgaaatt gccactgata ttgcctgtat catcagcaat catgaatcat taggaactat
      541 cgccaaatat tttaacattc ctttttttgt tttccaaaaa acaaaagaaa acaagctaca
      601 gcaggaacaa aaagaattag ccgtacttaa agattttgac gttgattgca ttgtgcttgc
      661 ccgctatatg cagatactat ctgcgcattt tgtttcacat tatcctaaca aaattattaa
      721 tatacaccat tcatttttgc cagcattcaa aggtgcaaaa ccataccacc aggcatatca
      781 gcatggtgtt aaaatcattg gagccaccag tcactatgtt accgaattgt tagaccaggg
      841 gcctattatt gagcaggatg ttatacgggt ttcgcataaa gattcaattg aagatatcat
      901 acgcaaaggc aaagatattg aaaaactggt actgtcccga gcacttaaaa aacatttgga
      961 acataaggta ctggtttatg caaataaaac aatagtgttt gattaaactt tatctgcttc
     1021 ccccaaggca tgcatcatat ttggcagcat gctcatcccg caaagggact tcacctaact
     1081 ctttatatat ttcaaacagc aaagaatatg cttcacagtg cgagctgtca attccaatgg
     1141 ccactttgca caggtcaatt gcctgcttga taaaatactc acgacgtttt ggatgtttaa
     1201 actcaaaata catatgggca agtgtcaaaa gaacatcagt atctttagga tatacttttt
     1261 tgcaatcttc aagaatctgg atagccatat caattttttt ctgtattctg taataatggg
     1321 aaagctgtac atacgcttcc ctgtttacag gatttatctc aagtatcttt ttataatgag
     1381 ccatagcaac attggtatta ttatcttttt gagcagcttg tgctttttca atttcaggtt
     1441 ttgtttgctg tagcgttatt gcattacaca caacaatatg ggaagctgca ctttcataat
     1501 ttttttgcag attatatata agaactaaat aataatgggc atacacatca tttttttggg
     1561 cgatagttct ttgtaaatgc tcaattgctt ttgaatattc actgaaaaaa taatataatt
     1621 ttccaacttc taaatgaatg tcaggctggt tgtcattgat ttttagagat tctacaaaat
     1681 attgtaaggc tctgacccta tcgttgtagt gatccagata aatttttgcc agtaagtttg
     1741 ctg
//
LOCUS       DAOSZP010000504         1734 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_51256_length_1734_cov_2.908874, whole genome shotgun sequence.
ACCESSION   DAOSZP010000504 DAOSZP010000000
VERSION     DAOSZP010000504.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1734
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51256_length_1734_cov_2.908874"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="PK348_09800"
     CDS             complement(<1..278)
                     /locus_tag="PK348_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase substrate binding
                     protein"
                     /protein_id="HXK66549.1"
                     /translation="MTNQDIRWIQRFNHFMNAFKRLEDAVTLYNDRTLSDLEEQGLIQ
                     AFEYTHELAWNVFKDFLEDRGVTNLYGSKDTTRYAFKNGLIDNGEVWM"
     gene            complement(307..1344)
                     /locus_tag="PK348_09805"
     CDS             complement(307..1344)
                     /locus_tag="PK348_09805"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HXK66550.1"
                     /translation="MQTQREKSKLDWQNLPFGYVKTDYNIRFYYKDGKWSEGELSTEE
                     TIPIHIAAPGLHYGQQAFEGLKVFETVDGRIVAFRPDENAKRMQRSCERIFIPEVPVD
                     MFIKAVDTVVAANAKFVPPFGTGASLYVRPVIYGAGARVGLGPADEYVFIVFVTPVGP
                     YYKGGFKPVKALVVEQYDRAAPNGVGDCKVGGNYAAGLRGGEYAKKKGFPIPLYLDPK
                     EKKYVDEFGTSNFIAIKGNTYLTPESNSILPSITNKSLMDIATLLGMNVEKRPIAIDE
                     VEHFDEVGAVGTAAVITPVCSIHYRDKVFTFCDEEKAGPTITKLYEYLTKLQTGEIND
                     TFGWLHEITVK"
     gene            complement(1491..>1734)
                     /locus_tag="PK348_09810"
     CDS             complement(1491..>1734)
                     /locus_tag="PK348_09810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXK66551.1"
                     /translation="DPVLADSISDLIVATQEKYRMTSLLVTQDLAVAQKTAHRVGLLH
                     NGMIVVDLESHEFFSKNNPYAKQFIDGDSEGPIDSM"
BASE COUNT          537 a          382 c          261 g          554 t
ORIGIN      
        1 tccatccata cttcaccatt atcaattaaa ccatttttaa aagcataacg tgtcgtatcc
       61 tttgaaccat ataaattagt aacacctctg tcttcaagga aatctttaaa aacattccat
      121 gctaattcat gggtatattc aaatgcctgt attaaaccct gctcttctaa atcagatagt
      181 gtcctatcat tatacaatgt aacagcatct tccaatcgtt taaaagcatt cataaaatga
      241 ttaaaacgtt gtatccatcg tatatcctga tttgtcatat ataaaattgc ctgattgtga
      301 tgtaatctat tttactgtaa tctcatgaag ccagccaaat gtatcgttta tttcaccagt
      361 ttgcaatttc gttaggtact catataattt ggtaatagta ggtcctgctt tttcctcatc
      421 acaaaatgtg aacactttat cacgatagtg gatgctgcat actggtgtaa taaccgctgc
      481 agttccaaca gcgcccacct catcaaaatg ctcaacttca tctattgcta ttgggcgttt
      541 ttcaacattc atacccaaaa gggtagctat atccataagg cttttgttcg ttatgctggg
      601 caaaatagaa tttgattcag gtgtgagata ggtattaccc ttaatggcta taaaatttga
      661 cgtaccaaat tcatcaacat atttcttttc ttttggatcc agatacagcg gtattgggaa
      721 acctttcttt ttggcatatt caccaccgcg caatcctgca gcataattac caccaacctt
      781 gcagtcaccc accccgttag gagctgctct gtcatattgt tcaacaacaa gagccttaac
      841 cggcttaaag ccgcctttat agtaaggccc aacaggagtc acaaaaacaa taaatacata
      901 ttcatccgcc ggtcccaacc caactcttgc tccagcaccg tatataactg gccgcacata
      961 caggctagca cctgtcccaa atggtggcac aaattttgca tttgctgcta ctactgtatc
     1021 aacagcttta atgaacatat caacaggcac ttcaggaatg aaaatccttt cacaactgcg
     1081 ctgcattcgt ttcgcattct catcgggtct gaaagctaca attcgaccat ccacagtttc
     1141 aaagacttta agcccttcaa acgcctgctg tccataatga aggccaggtg cagctatatg
     1201 aataggtata gtttcttcgg ttgaaagctc tccttcagac cacttcccat ctttataata
     1261 aaatctgata ttataatctg tttttacgta tccaaatggt agattttgcc agtctaactt
     1321 agatttttct cgttgtgttt gcatagctca taaaccctca taaaaaaaaa taaaaaccat
     1381 agcctttaat atggttaaat agcttaaaca tgtttatatt attttaccaa cataatagta
     1441 cagtgtcaaa tatattttaa ttgaaatatt attaaggtta tgttaatatt ttacatacta
     1501 tctattggtc cttcgctatc tccatcaata aactgctttg catatggatt attctttgaa
     1561 aaaaattcat gagattccag atcaacaaca atcatgccat tatgcagcaa tccaacccgg
     1621 tgagctgtct tttgagcaac agctaaatcc tgcgttacca gtaaagatgt catcctgtat
     1681 ttttcctgag tggcaacaat aagatcagaa attgaatcag caagaaccgg gtca
//
LOCUS       DAOSZP010000505         1733 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_51281_length_1733_cov_3.575685, whole genome shotgun sequence.
ACCESSION   DAOSZP010000505 DAOSZP010000000
VERSION     DAOSZP010000505.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1733
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51281_length_1733_cov_3.575685"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1063
                     /locus_tag="PK348_09815"
     CDS             <1..1063
                     /locus_tag="PK348_09815"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HXK66552.1"
                     /translation="RWKDKVINASHTTPESKDLQEIMSIMVLDGVDCIIMEVSSHALS
                     LLRVDDIHFDCAIFTNLTRDHLDYHITFNDYFMAKVRLFEILNKSLKQKKAAFINADD
                     NYGKQILNWRERYGYPLFSFGITNNTDYHCIESSIQISIKGTQFATDLPFAKQFTMKL
                     AGRFNVYNALGAIGVADFLNVPVGRIVTGISVLSSVPGRFDTILSPEGFAVVVDYAHT
                     NDALEKLLLSVRSLKPERIITVFGCGGNRDKTKRPLMGKVSEDYSDWVIVTSDNPRKE
                     NPEAIIRDITTGMKTNRYQVIVNREEAIKHAIYMAKQDDVIVIAGKGHEDYQIIGTKK
                     YHFDDREVARKYINERLGK"
     gene            1060..>1509
                     /locus_tag="PK348_09820"
     CDS             1060..>1509
                     /locus_tag="PK348_09820"
                     /inference="COORDINATES: protein motif:HMM:NF013396.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HXK66553.1"
                     /translation="MIVRFETTSQTIATAINAIHHGSGQPVQYITTDSRLIEDKSLFV
                     PITGNTFDGHDFIEDVCKTGKCTSFLTHKQDKVNIADRYNTSAIYCNDTLQAYAAIAR
                     WHRKKFNIPVIGVTGTNGKTTTKEMIAQVLSTEGQCLKTQKNYNNEIG"
     assembly_gap    1510..1519
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          572 a          308 c          368 g          475 t
ORIGIN      
        1 tcgctggaag gataaagtaa tcaatgcttc acatacaaca ccagagtcaa aggacctgca
       61 ggagataatg tcaataatgg ttctggatgg cgttgattgt ataataatgg aagtttcttc
      121 ccatgcacta tcgctgttac gggttgacga tatacatttt gattgtgcca tatttaccaa
      181 tctcacgcgc gatcatttag actatcacat taccttcaat gactatttta tggcaaaagt
      241 gcgtttgttt gaaattttaa ataaaagcct taaacagaaa aaagcagcat tcattaatgc
      301 tgatgataat tatggcaaac agatattaaa ctggcgcgag cgctatggtt atccactttt
      361 ctcctttggt atcaccaaca atactgatta tcattgcatt gaatcttcaa ttcaaatttc
      421 aattaaaggc acgcaatttg ccacggattt accatttgcc aaacaattta ccatgaaact
      481 ggcaggccga ttcaatgtgt acaatgcatt gggagcaatt ggtgttgctg attttttaaa
      541 tgtgcctgtt ggcagaatag taacagggat atcagtgcta tccagtgttc ccggcagatt
      601 tgacacaatt ctgtcgcctg aaggttttgc agtggttgtt gattatgcac ataccaatga
      661 tgcactggaa aagttattgc tttcagtacg ttcattaaaa cctgagcgca ttataacggt
      721 atttggctgt ggtggtaaca gggataaaac caaacgacca ttaatgggca aagttagcga
      781 ggattattct gactgggtta ttgtaaccag tgataatccc agaaaagaaa atcctgaagc
      841 tataatacgg gatattacaa caggaatgaa aacaaaccgt tatcaggtaa ttgttaatcg
      901 tgaagaagca atcaagcatg caatatatat ggcaaagcag gatgatgtta tagtcatagc
      961 gggcaaaggc catgaggatt atcagattat tggcacaaag aagtaccact ttgatgacag
     1021 ggaagttgca cgcaaatata tcaatgagag gcttgggaag tgatagtacg gtttgaaaca
     1081 acctcacaaa ccattgctac tgccattaat gctattcatc acggcagtgg tcagcctgtg
     1141 cagtatatta ctacagattc gcggcttata gaagataaaa gcctttttgt gccaataact
     1201 ggaaacacat ttgatgggca tgattttata gaagatgtat gtaaaactgg aaaatgcacc
     1261 tcatttttaa cgcataaaca ggataaagta aatatagcag atcgttataa tacaagcgcc
     1321 atttattgca atgacacatt gcaggcgtat gcagcaatag cacggtggca caggaaaaaa
     1381 tttaacatac cagttattgg tgttaccggt accaatggca agacaaccac aaaagagatg
     1441 atagcacagg ttctttcaac agaaggccag tgcctgaaaa cccagaaaaa ttataataat
     1501 gaaattggcn nnnnnnnnnt gcagaataca cacagctatg ccgttattga gatgggtatg
     1561 aatcatcctg gcgagatatc acgtctttca cagattgcca tgccaaccat agctgtaatc
     1621 accaacatag gggaggggca tcttgaattt ttaggatcag ttgaaaatgt tgcacatgca
     1681 aaagctgaga tactggatgg tatggctgca gggagcatat gcatactgaa taa
//
LOCUS       DAOSZP010000506         1732 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_51332_length_1732_cov_3.555158, whole genome shotgun sequence.
ACCESSION   DAOSZP010000506 DAOSZP010000000
VERSION     DAOSZP010000506.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1732
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51332_length_1732_cov_3.555158"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            180..818
                     /gene="jag"
                     /locus_tag="PK348_09825"
     CDS             180..818
                     /gene="jag"
                     /locus_tag="PK348_09825"
                     /inference="COORDINATES: protein motif:HMM:NF041568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HXK66554.1"
                     /translation="MKVLDIEGKTVEEATKKALSLLKINDMNKINIEVLDEGKSGIFG
                     FGISRPAKIRVYYQQEDDIGQQAKEIIEKILMLMEVDAKVKDYKESENKVYVELESSS
                     SGLIIGKKGKTLEALQFMVNLLVNKMTNSEKKIILDIESYRAKREKALRKLSKEIALK
                     VARTGKPWTLEPMNPFERRLIHLTLQNDSKVTTKSEGQGIYRKVKIMPVEKR"
     gene            824..>1732
                     /gene="mnmE"
                     /locus_tag="PK348_09830"
     CDS             824..>1732
                     /gene="mnmE"
                     /locus_tag="PK348_09830"
                     /inference="COORDINATES: protein motif:HMM:TIGR00450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HXK66555.1"
                     /translation="MDDTICAPATPPVHSPIAIIRISGPDSLRVASSIFRAQQHTQEF
                     IPRYAYYGIIHDNNEEIDDCIVIYYKHPQSYTGEDMVEIFCHGNPIIVNKIINCIVAL
                     NVRIAEPGEFTKRAFLHGKMDLTEAEAINHIIAARSEWEITTAIQQKHGSLKNAINDI
                     KQHIIELKADIECAIDFVDEDIEIISFENAQQRMHTIINKIQDILNRCRIGQHLSHGI
                     DIAIVGKPNAGKSSFLNCILNHERAIVSDTPGTTRDVIKESVSIKGIQCNLLDTAGIH
                     KKADGIELLGVQRSYRLIDEVSLIIFI"
BASE COUNT          623 a          268 c          377 g          464 t
ORIGIN      
        1 ttaatatatt aatgaggtta tgcagataag cgattttgtt agatcatttt gatcaacatg
       61 ttatacagta tttgagatta aagtactaag gtgaaatatt tataattaaa aggagggaag
      121 atacatcacc atacaaacaa ctgaactatt attatcaaag tttcgtagga ggcgtgaaca
      181 tgaaggtgtt agatattgaa gggaaaactg ttgaagaagc aacgaagaaa gccttatcat
      241 tattaaaaat taatgatatg aacaaaatca atattgaagt gctggatgaa gggaaaagcg
      301 ggatctttgg ttttggcata tcacgccctg ctaaaatacg agtatattac caacaggaag
      361 atgatattgg ccaacaggca aaggaaatta ttgaaaagat tttgatgctg atggaagttg
      421 acgcaaaggt taaagattat aaggaaagcg aaaataaagt atatgttgaa ctggaaagct
      481 caagttcggg attaattata ggtaaaaaag ggaaaaccct ggaggcattg cagtttatgg
      541 ttaaccttct ggtcaataaa atgaccaatt cagaaaaaaa gatcatactt gatattgaat
      601 catacagggc aaaaagagaa aaagcgttgc gtaaattatc caaagaaatt gcattgaaag
      661 ttgcccgaac gggtaagcca tggacgttag aacccatgaa tccatttgaa cggcgtctta
      721 tacatttgac attgcaaaat gattcaaagg ttaccaccaa gagtgaggga cagggcattt
      781 acagaaaggt aaaaattatg cctgtggaaa aaaggtaatg cagatggatg atacgatatg
      841 tgctccggca actccgccgg tacattcacc aatagccatt atacggataa gcggccctga
      901 ttcattacgg gtcgcttctt ctatattcag ggcacaacag catacacagg aatttattcc
      961 gcgatatgcg tattatggta tcattcatga taacaatgaa gaaattgatg attgtattgt
     1021 aatctattat aaacacccgc aaagctacac aggtgaggac atggtggaaa tattctgtca
     1081 tggcaatccg atcatagtaa ataaaataat taattgtatt gttgcactca atgtgcggat
     1141 agctgaacca ggtgagttta ccaaacgtgc atttttacat ggcaagatgg acctgaccga
     1201 agctgaggct ataaaccata ttatcgctgc acgcagtgaa tgggagataa caactgcaat
     1261 acagcaaaag catggatcac tgaaaaatgc aatcaatgat attaaacagc acatcattga
     1321 attaaaagca gatattgaat gtgctattga ctttgtggat gaggatattg agattatatc
     1381 gtttgaaaat gcacagcaaa ggatgcacac gataattaat aaaatacagg acattcttaa
     1441 tagatgcagg attggccagc acctttctca cggcattgat attgcaattg ttgggaagcc
     1501 caacgcaggt aaatccagct ttttgaactg tatactcaat catgaacgag caattgtgtc
     1561 agatacgcca gggacaaccc gcgatgtgat aaaagaatcg gtttcaatta aaggtattca
     1621 atgcaacttg cttgatacag ctggtataca taaaaaagca gatgggattg aacttttggg
     1681 tgtgcagcgc agctacaggc tgatagatga ggtgtctctt ataattttta tc
//
LOCUS       DAOSZP010000507         1725 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_51617_length_1725_cov_3.076647, whole genome shotgun sequence.
ACCESSION   DAOSZP010000507 DAOSZP010000000
VERSION     DAOSZP010000507.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1725)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1725)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1725
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51617_length_1725_cov_3.076647"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="PK348_09835"
     CDS             <1..307
                     /locus_tag="PK348_09835"
                     /inference="COORDINATES: protein motif:HMM:NF024544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagella basal body P-ring formation protein
                     FlgA"
                     /protein_id="HXK66556.1"
                     /translation="CIKNYVKGLTIIHGYSVKMITGDGSFTNNQDSYNELSPMIKRRT
                     VSIGDYVMVKLIKKSIAIELMGRSLQSGTTGDEITVTLQNGKVVTGTIHNDAIECKL"
     gene            304..930
                     /locus_tag="PK348_09840"
     CDS             304..930
                     /locus_tag="PK348_09840"
                     /inference="COORDINATES: protein motif:HMM:NF014195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HXK66557.1"
                     /translation="MKKIFMAIALVIVSTVYADTIWKDKNLYTAQNAIKVGDIFTIVV
                     SDISQMRFTLTLANNDAFTVTSAPDGTITPFLPQVNASKHATKNNTTNLQHRSNIVIA
                     IAATVQERLANGNFRVNGTKTFSFSGVTTIFNVSGIVNPASINGGTINSSDIANLVLQ
                     IQGVKEGVRITRTPLKEGESASSALTEQEKQQIIIQYLQRILGELTQQ"
     gene            933..>1725
                     /locus_tag="PK348_09845"
     CDS             933..>1725
                     /locus_tag="PK348_09845"
                     /inference="COORDINATES: protein motif:HMM:NF014206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HXK66558.1"
                     /translation="MKMRNKIIILCTLLLLFFQVNLYAQVSVKIKDLIAIDGLKENQI
                     YGYGLVIGLQGTGDSKVALTRSSLSNVLKNLGLNNETINSKNIAAVLVTATLPPYVRV
                     GDRIDVTVSSIGDAKSLEGGVLLQSSLKGADDATYVVAQGMLSIAGDKRKGATIGKIV
                     SGGIVEREILPEVVVNGTIKLALLNFDFSVAKEIKSAIEKKYPDARPVIDKGFIQCSL
                     PQNITAAEFISTIENIEVVPVYSARVVVNESNGTIVMGGDIRVSQA"
BASE COUNT          582 a          287 c          347 g          509 t
ORIGIN      
        1 gtgtataaaa aattatgtaa aagggcttac tattatccat ggctactctg ttaaaatgat
       61 aaccggtgat ggttcattta caaacaatca ggattcatac aatgaactat cgcccatgat
      121 aaaacgccgt accgtaagca ttggtgatta tgtcatggta aaacttatca agaaatcaat
      181 tgctattgag ttgatgggca ggtcgctaca aagtggtaca acaggcgatg aaattacagt
      241 gacattacaa aatggcaagg ttgtaaccgg aacaattcat aacgatgcta ttgagtgcaa
      301 gctatgaaaa aaatttttat ggcgatagct ctggtaattg tatcaacagt atatgctgat
      361 acaatatgga aagataaaaa cctttatacc gcacagaatg ctattaaagt aggcgacata
      421 tttaccattg tggtttctga catatcgcag atgcggttta cgttgacgct tgccaataac
      481 gatgctttta cggttacttc tgcgcctgat ggcactataa cgcctttttt acctcaggta
      541 aatgccagca aacatgctac caaaaataat accacaaatt tgcaacacag aagtaatata
      601 gtaatcgcaa tagctgcaac agtacaggaa cgccttgcaa atggtaattt cagagtaaac
      661 ggcacaaaaa cattttcctt tagtggagtt acaaccattt ttaatgtctc aggaattgta
      721 aatcctgctt ccataaacgg cggtactatt aattccagtg acatcgccaa tttggtatta
      781 cagatacagg gtgttaaaga aggcgtacgt ataacacgca ccccattgaa agagggcgaa
      841 tctgcctcat ctgcattaac cgaacaggaa aaacagcaga taataattca atatttacaa
      901 agaatattag gtgaactaac acagcaataa ctatgaaaat gagaaacaaa ataattattt
      961 tatgtacctt gcttttatta ttttttcagg ttaatcttta tgcacaggtt tctgtaaaga
     1021 ttaaagattt aattgccatt gatggattaa aggaaaacca gatatacggt tatgggcttg
     1081 ttataggttt gcagggtaca ggcgatagca aggttgcgtt aacccggagc tcgctttcaa
     1141 atgttttaaa aaatttaggg cttaataatg aaactattaa ttcaaaaaat attgctgctg
     1201 tgcttgttac tgcaacattg ccaccatatg tcagggtagg cgatcgtatt gatgttactg
     1261 tgtcatctat tggtgatgca aaatcacttg aaggcggtgt gctattacaa tcatcactaa
     1321 aaggagctga tgatgctact tatgttgtag cacagggcat gctatctatt gccggagata
     1381 aacgtaaagg tgcaaccatt ggaaaaattg tttctggcgg tattgttgaa cgtgaaatat
     1441 tacccgaggt tgttgttaat ggaactatta aacttgcatt attaaacttt gatttttcag
     1501 ttgcaaaaga aatcaaatca gcaattgaaa aaaaatatcc agatgccagg ccagtcattg
     1561 ataagggttt tatacagtgc agtttacctc aaaatataac tgctgcagaa tttatatcta
     1621 ctatagaaaa tattgaagta gtgccagtat atagtgcccg tgtcgttgtt aatgaaagca
     1681 atggcacaat agttatggga ggtgatatca gggtgtcaca ggctg
//
LOCUS       DAOSZP010000508         1723 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_51691_length_1723_cov_3.261990, whole genome shotgun sequence.
ACCESSION   DAOSZP010000508 DAOSZP010000000
VERSION     DAOSZP010000508.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1723
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51691_length_1723_cov_3.261990"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="PK348_09850"
     CDS             <1..201
                     /locus_tag="PK348_09850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66559.1"
                     /translation="KDVPLQVNRQLYMVFSKEARSSGITLNEQYMFWPLHTIAGIILP
                     HTGENTCQSCTLLHCQFRNILT"
     gene            257..1405
                     /gene="alr"
                     /locus_tag="PK348_09855"
     CDS             257..1405
                     /gene="alr"
                     /locus_tag="PK348_09855"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HXK66560.1"
                     /translation="MILTHPAYCNRPTRAEISIPNLLNNFSIVRALVAPHVKIMAMVK
                     ANAYGHGIVRVSQELIKAGVDYLGVAYLEEALFLRQHGISTPILVCGAINTEQIPEFI
                     LNDVAITSSSLDKSKAIHNAAKALGKKAKVHLKIDTGMERIGVHWYNAENFINETMSL
                     DGIEVTGIFSHLAKAESDADFTKQQIQRFATIIEYMHKHSICPPYVHLANSAAIINHP
                     ESHFTMVRPGIMLYGYNPNGYNPDITFNGKKLLPVMTLKTKVSYFKVVPAHTGISYNH
                     LYTTSKQTRIVTLPVGYGDGYSRLLSNKGEVIIRGKTYPVVGAVCMDQIMVDIGPDGT
                     AYNGDDVLLFGQMDGYSIPLESLCQKIGTITYEILTNISPRVPRIYIE"
     gene            complement(1407..>1723)
                     /locus_tag="PK348_09860"
     CDS             complement(1407..>1723)
                     /locus_tag="PK348_09860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66561.1"
                     /translation="NSVNNDEHAIKELQKRRGWYDEKLLLNFIENVAKSKEIKKKYEL
                     TTVAIPELKEGMYLAEDVMSKNGVIIGTNKQKITTSLLVTLKNYYNKKLINPFIKVLL
                     VK"
BASE COUNT          539 a          353 c          306 g          525 t
ORIGIN      
        1 aaagacgttc cgttacaggt aaacagacaa ttgtatatgg tatttagcaa agaagcccgt
       61 agcagtggaa tcacgcttaa tgagcagtat atgttctggc cacttcatac cattgctggt
      121 atcatactac cacacacggg ggaaaacacc tgccagagct gcacgttact acactgccag
      181 ttcagaaata tcttgacata ataggtcatt taacaactga taacaaccat taaatctata
      241 ccataaacat tgaaccatga tattaacaca tcctgcatac tgtaaccgcc ccacacgggc
      301 tgaaatttca atacctaatc tactcaacaa tttttcaatt gtgcgggcac tggttgcgcc
      361 acatgtcaaa attatggcaa tggtaaaagc aaacgcatat ggacatggca ttgtgcgcgt
      421 ttcacaggaa ctgataaaag caggtgttga ttatttaggt gttgcctact tagaagaagc
      481 gttgttttta cgccagcacg gcatatcaac accaatactg gtgtgcggtg ccattaacac
      541 tgaacaaatc cccgaattta ttttaaatga tgttgccatt actagctctt cactggataa
      601 atcaaaggca attcacaatg cagcaaaagc actgggcaaa aaagcaaagg tgcacctaaa
      661 gatagacacg ggtatggaac gcataggtgt acactggtac aatgcagaaa attttattaa
      721 tgaaaccatg tcacttgatg gcattgaagt cactggcatt ttttcacatc ttgcaaaagc
      781 tgaatctgat gcggatttta caaaacaaca gatacaacgg tttgctacca taattgagta
      841 tatgcacaaa cattctatat gtccaccgta tgtacaccta gctaattcag cagcaatcat
      901 taatcatcct gagtcacatt tcaccatggt acgccctggc attatgctgt atggctataa
      961 tcccaatgga tataatcccg atattacatt taacggcaaa aagttactgc cggtaatgac
     1021 actgaaaaca aaagtttctt actttaaagt ggtacctgca cacactggaa ttagctacaa
     1081 ccatttatat accacgtcaa aacaaacacg tatagtaacg ctgcctgttg gttacggtga
     1141 tggctacagt cgtctgcttt ccaataaagg tgaagttatt attcgaggaa aaacatatcc
     1201 tgtggttgga gctgtatgta tggaccagat tatggtggat attggacctg atggtactgc
     1261 ctacaatggt gatgatgtgc tactgtttgg ccagatggat ggttacagta ttccccttga
     1321 atcactatgt caaaaaattg gcaccataac ctatgaaatt ttaacaaaca ttagcccccg
     1381 tgtaccacgt atatatattg aataacttat tttactaata gtactttaat gaagggattt
     1441 attaattttt tattataata attcttaagc gttaccagca aagatgttgt gattttttgc
     1501 ttatttgttc caattatgac tccgttttta ctcattacat cttcagcaag atacatccct
     1561 tctttcagtt caggtattgc tactgttgtt aactcatatt ttttcttaat ctccttgctt
     1621 ttggcaacat tttcaataaa gtttaatagc aatttctcat cgtaccagcc tcttcttttc
     1681 tgcagttcct taatggcatg ctcatcattg ttcacagaat taa
//
LOCUS       DAOSZP010000509         1723 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_51700_length_1723_cov_2.642686, whole genome shotgun sequence.
ACCESSION   DAOSZP010000509 DAOSZP010000000
VERSION     DAOSZP010000509.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1723
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51700_length_1723_cov_2.642686"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="PK348_09865"
     CDS             <1..273
                     /locus_tag="PK348_09865"
                     /inference="COORDINATES: protein motif:HMM:NF037386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HXK66562.1"
                     /translation="KKATKKNRYDMLMNLQQEISRERLMETLGDTVQVLVEEKVDRYN
                     YIGRTQYDAPEIDGIFYLTAYNVRVNDIVTAIITDTLEYDRIGEMI"
     gene            270..923
                     /gene="pgsA"
                     /locus_tag="PK348_09870"
     CDS             270..923
                     /gene="pgsA"
                     /locus_tag="PK348_09870"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HXK66563.1"
                     /translation="MNIPNLLTLSRIILIPLFLYLIFTPTIEHRIWALVIFIIASFTD
                     FLDGWTARKLRQETDTGKFLDPLADKFLVISALIAFLFLDPLIPLWMVIVIIARDLLI
                     TLMRYLSIKKGSMLRTSRLGKFKTAFQMIGIIVIIMVFIVRNSIKNVQLEINQLSALK
                     LETVVEILNSNLVHKWLIVCPYFIMAVVTFLTALSGLRYIVTNWRLFLPPYTKKDAK"
     gene            930..1409
                     /locus_tag="PK348_09875"
     CDS             930..1409
                     /locus_tag="PK348_09875"
                     /inference="COORDINATES: protein motif:HMM:NF016489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HXK66564.1"
                     /translation="MNWKEFLFTGLYTGYFPKGSGTVGALLALLLYVILHQLCGRYGM
                     LANVVLVAFITYPAIKLGDDAEIYFAQKDPQEVVLDEMLGFWVTMLFHPFSWSTAVLG
                     FFFFRLFDIIKPYPIRKLEHFRGGLGIMIDDIIAGVYANITIAIIMVIMKAYGIVLV"
     gene            1471..>1723
                     /locus_tag="PK348_09880"
     CDS             1471..>1723
                     /locus_tag="PK348_09880"
                     /inference="COORDINATES: protein motif:HMM:NF024827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HXK66565.1"
                     /translation="MKRTYTIIGVFAIILLTFYIAYAQKTMGDSSHKKNYCIECHIKL
                     NGKPRAVVLEWENSIHAKNNLECHICHGGNPDADDAKAAK"
BASE COUNT          532 a          274 c          350 g          567 t
ORIGIN      
        1 aaaaaggcaa caaagaaaaa tcgctacgat atgctgatga atttgcagca ggaaatttca
       61 cgggaaaggt tgatggaaac tctcggtgac acagtgcagg tgcttgtgga agaaaaagtg
      121 gatcgttata attacattgg aagaactcaa tacgatgcac ctgaaataga tggcattttt
      181 tacttgaccg catacaatgt acgtgtcaac gatattgtga ctgcaataat aactgataca
      241 ttagaatatg accgtatagg agaaatgatt tgaatatacc taatttatta acattatcac
      301 ggataatact cataccatta ttcttgtatt taatctttac accaactata gaacacagaa
      361 tctgggcact ggttattttt atcatagcat cgtttactga ctttttagat ggatggacag
      421 cccgcaagtt gcgtcaggaa actgatacag gaaaattttt ggacccgctt gctgataagt
      481 ttctggttat ttcagcgctt attgcattct tattccttga tccgcttata ccgttatgga
      541 tggttattgt tatcatcgcg cgtgaccttt tgataacatt gatgcgctat ttatctatca
      601 aaaaaggatc aatgttacga acaagcaggt taggtaaatt taaaacagca ttccagatga
      661 ttggcattat cgttataata atggtgttta ttgtccgtaa ttccataaaa aatgtacaat
      721 tggaaattaa ccagttaagt gccctgaagc ttgaaacagt tgttgagatt cttaattcaa
      781 atttagttca taaatggctt attgtatgcc cctatttcat tatggctgtg gtaaccttct
      841 taacagcttt atcaggcctg cgatatattg taaccaactg gcgtttgttt ttaccaccat
      901 acacaaaaaa ggatgctaaa taaatttata tgaactggaa agagtttttg tttacagggc
      961 tttatactgg ctacttcccc aaaggttcgg gtactgttgg cgcgctgtta gctttgttgt
     1021 tgtatgtaat tttacatcaa ctatgtggta gatacggaat gttagccaat gttgtgcttg
     1081 tagcgtttat aacctatcct gcaatcaaac tgggtgatga tgcagaaatt tattttgcac
     1141 aaaaagaccc gcaggaagtg gttcttgatg aaatgcttgg tttctgggtt accatgttgt
     1201 ttcatccatt tagctggagt acagcagtat tgggtttctt ttttttcagg ctgtttgata
     1261 ttattaaacc ttacccaata cgcaaactgg agcattttcg tggagggcta ggtataatga
     1321 ttgacgatat cattgccggg gtgtatgcta acattaccat tgccattata atggttatta
     1381 tgaaagctta tgggattgta cttgtgtaat gagtacaggg tgtcgaaaaa gaaaataggc
     1441 atacaaagaa acattgcttt gaggttagtt atgaagcgta catatacaat tattggtgtt
     1501 tttgctatta tacttttaac tttttatatt gcatatgctc aaaagacaat gggcgatagt
     1561 tctcataaaa aaaattattg tatagaatgt catattaaac tcaatggcaa acctcgtgca
     1621 gtagttcttg agtgggaaaa cagcatacat gcaaaaaata atcttgaatg ccatatatgc
     1681 catggtggaa atcccgatgc agatgatgct aaagctgcaa agg
//
LOCUS       DAOSZP010000510         1718 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_51892_length_1718_cov_3.728202, whole genome shotgun sequence.
ACCESSION   DAOSZP010000510 DAOSZP010000000
VERSION     DAOSZP010000510.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1718
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51892_length_1718_cov_3.728202"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..894)
                     /locus_tag="PK348_09885"
     CDS             complement(<1..894)
                     /locus_tag="PK348_09885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HXK66566.1"
                     /translation="MSSLIVLLLNRALRDQISEVFTQKGFQLSLVERDECSKESINKS
                     TPDIVLFDVGMHSEDDFLLYLDVVRWSEVPVLPVIYRKENSHCTMLKAHELLKDVEAI
                     LLGTDYSDQKDNLAAVQQYPYALTHYHDIFETIPYAIFIHDIHTGEVLDVNVAATELF
                     GYSRDELMYENPAVAYPHNNEFTLSNAMKHIDVAASKGAHTFEWCAYKKSGQVMWLKI
                     HLTLVSISGSKQIMAVVHDITRERQLQKALEASEKRFKSVFEEFSDGLMIIDKEGIID
                     CNTAVEVMFGYSKDELIGLHPA"
     gene            complement(1141..>1718)
                     /locus_tag="PK348_09890"
     CDS             complement(1141..>1718)
                     /locus_tag="PK348_09890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66567.1"
                     /translation="YNQYHRLTVTPYIYYMRQDELYLQNLLRGTLFYTFTFNPYINPY
                     HKSQVDTVVVEKEDGLRPILFRETVGASFVYSYFSGRLGFGFEKQTQDPEKDLLYGAE
                     SIFQIQIPFWDYFTYTFNVDNFISAPEGGDSSYQIRTDIKNAVSIKINTWLSLTIRHR
                     WFYYNPLDTQIPYTYTQLLISLDVKAKFKVF"
BASE COUNT          543 a          352 c          286 g          537 t
ORIGIN      
        1 tgcaggatga aggccaatca attcatcttt agaatagcca aacattactt caactgcggt
       61 attgcagtca ataatgccct ctttatcaat aatcattagc ccatcagaaa attcttcaaa
      121 tactgactta aatcgctttt cacttgcttc cagtgctttc tgtaactgcc gttcacgggt
      181 aatatcgtgc accactgcca ttatctgttt gcttccagat attgaaacca acgtcagatg
      241 tattttaagc cacatcacct ggccactctt tttgtacgca caccactcaa aggtatgagc
      301 accctttgat gcagctacat caatatgctt cattgcattt gatagtgtaa attcattatt
      361 gtgtggataa gcaacggcag gattttcata catcaattca tcccgtgaat acccaaataa
      421 ttccgtagca gccacattga catcaagcac ttcaccagta tgtatatcat ggatgaatat
      481 tgcatacggt attgtttcaa atatatcatg gtaatgtgtc agggcgtacg gatactgctg
      541 tactgccgca agattatctt tctggtcaga ataatccgtt ccaagtaaaa ttgcttcaac
      601 atcctttaac aattcatggg cctttaacat ggtacagtgg gaattttctt tacggtatat
      661 taccggcaat accggcactt cactccagcg tacaacgtct aaatacagta aaaagtcatc
      721 ctcactgtgc atgcccacat caaacaacac aatatcagga gtacttttgt taatggattc
      781 tttactgcat tcatcgcgtt ccaccaaaga caattgaaat cccttctgtg taaaaacctc
      841 agaaatctgg tcacgcagcg ctctgtttag caataatact attaagcttg acattgatac
      901 ctcttttttt aggcatttgc ctaatttacg aaatttaaat aattaaatca acaaaaaatt
      961 atactcaaag cacatttctt gtttgcgtag tgatttttta gggatgtcag gaagaataat
     1021 acgtgggatt acggtgagtg catactattt gaaaataata tatgcagcca gttatctatt
     1081 cagttactat cgctaaatat caccattaac aatataccac actataatga ataaaacaag
     1141 ttaaaatact ttaaactttg cttttacatc aaggcttatt aaaagctggg tataggtata
     1201 tggtatttgc gtatcaagtg gattatagta aaaccacctg tgtctaattg ttaatgaaag
     1261 ccatgtatta attttaatgc ttacggcatt ttttatatca gtccttattt gataagaact
     1321 atcgccacct tcaggagccg agataaaatt atctacgtta aaggtgtatg taaaataatc
     1381 ccagaatggt atctgtatct gaaaaatgga ttcagcacca tagagtaaat ctttttcagg
     1441 atcctgcgtc tgcttttcaa atccaaagcc taacctgcca ctgaaatagc tatatacaaa
     1501 agaggcacca accgtttcac gaaacaatat cgggcgcaac ccgtcttctt tttcaaccac
     1561 caccgtatca acctgagatt tgtgatacgg atttatatat gggttaaatg taaacgtata
     1621 aaaaagcgtc ccgcgcagta aattttgcaa gtatagctca tcctgccgca tgtaatagat
     1681 atagggagtg actgtcaatc ggtggtactg attataga
//
LOCUS       DAOSZP010000511         1717 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_51942_length_1717_cov_2.764741, whole genome shotgun sequence.
ACCESSION   DAOSZP010000511 DAOSZP010000000
VERSION     DAOSZP010000511.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51942_length_1717_cov_2.764741"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..459
                     /locus_tag="PK348_09895"
     CDS             <1..459
                     /locus_tag="PK348_09895"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66568.1"
                     /translation="EDFIDGREFHVTLWGNGVIHTLPPAEMDFSAFDNIKDRVCTYNS
                     KFKPGTSHYENITLKIPAQLDNNQLAILNETALQAYRALGCRDYARIDIRLKDGIFYI
                     LDINPNPDFSPDTSTVYTAEHAGLSYGAFASCLINLAALRHHVYGEKITI"
     gene            complement(504..719)
                     /locus_tag="PK348_09900"
     CDS             complement(504..719)
                     /locus_tag="PK348_09900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66569.1"
                     /translation="MKDYLIELISSMFWIVWFVWMSDTEIILNVYVWLGIILFVGWYF
                     STDKKIPVTVQIAVWLCFFILDILFRW"
     gene            complement(801..>1717)
                     /locus_tag="PK348_09905"
     CDS             complement(801..>1717)
                     /locus_tag="PK348_09905"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HXK66570.1"
                     /translation="REKGVVTVQEVVSKFEVTPRTVKRDIEYLRERVNAPIQYISHKK
                     GYCYSLPFDYFTYRNERMLLFYIFLTRLIDSPTHFPFIDRTVISNIKKSIKATYLPLI
                     TRITYEIEEWEELDIELFSKLFAAMRDGKVVTIQYADVKGKPSTRAIAPEHFIHYEGR
                     WYCIAYDEHKKGIRTFLLSRIRKAQVDVKRRSKKLSNEEIQKYIQSTYGIYKGKTGQV
                     VCIRFYEPVSNIVKKQVWHREQKMKEGSHPQFGAYVEMLLPVHHPQELLGKILRYGKY
                     AEVISPEDIRQQWVNAIAETYKRYCEKK"
BASE COUNT          522 a          371 c          285 g          539 t
ORIGIN      
        1 gaagatttca ttgatggcag ggaatttcat gtgacattat ggggcaatgg cgttattcat
       61 accctccctc ctgcagagat ggatttcagc gcgtttgata atatcaaaga cagggtatgt
      121 acctataatt caaaattcaa acctggtaca tcacattatg aaaatatcac gttaaaaatc
      181 cctgcacagc tggacaataa ccagcttgca atattgaatg aaacagcgtt acaagcatac
      241 agagctcttg gctgtcggga ttacgcacgc atcgatattc gcttaaaaga cggtattttc
      301 tatattcttg acattaatcc caaccctgac ttcagccctg atacatcaac agtatacaca
      361 gctgagcatg ctgggctttc gtatggtgca tttgctagct gcctgataaa tttagcagca
      421 ttgcgccatc acgtttacgg ggaaaaaata acaatataat ccattaccat taacagtcat
      481 tataagttca taaatccctt taattaccac ctgaaaagaa tatctaatat aaagaagcac
      541 agccaaacgg ctatttgcac agttacaggt attttcttat cagtggaaaa ataccacccc
      601 acaaacaaaa ttattcctaa ccagacatat acattaagga taatttcagt atcgctcatc
      661 caaacaaacc atactatcca gaacattgac gatatcaatt ctataagata gtctttcata
      721 tacacccttc cacgataata tatttattaa aattatgaaa aaacttataa ggcaagcagg
      781 gacatacaat gtccctgata ttattttttt tcacaatacc gtttataagt ctctgctatg
      841 gcattaaccc actgctggcg tatgtcttca gggcttatga cctcagcata tttgccatag
      901 cgcaaaattt tccctaaaag ctcctgtggg tgatgaacgg gcaatagcat ttccacatag
      961 gcgccaaatt gaggatgact gccctccttc atcttttgtt ccctgtgcca gacctgtttt
     1021 ttaacaatat tactaacagg ctcgtagaac cgtatgcaca ctacctggcc tgttttacct
     1081 ttataaattc cataggtaga ttgtatgtat ttttgaattt cctcgtttga aagtttttta
     1141 ctgcgccttt taacatctac ctgtgctttc ctgatgcgcg aaagcaaaaa tgtccgtatt
     1201 ccttttttat gttcatcgta agctatacaa taccacctgc cttcataatg aataaaatgt
     1261 tcaggggcga tagctcttgt acttggctta cccttaacat cagcatactg tatggttaca
     1321 accttaccgt cacgcattgc cgcaaacaat ttactgaaca gttctatgtc cagctcctcc
     1381 cattcttcta tctcatatgt tattcgtgta atgagcggta ggtaagtagc tttgatagac
     1441 ttttttatat tgcttattac agttctatct ataaacggga aatgtgttgg gctatcaata
     1501 agacgagtta gaaatatata aaacaacagc atgcgttcat ttcggtaggt aaaataatca
     1561 aacggcaatg aatagcaata ccctttttta tgggatatat actgtatggg ggcattaact
     1621 ctttctctga gatattcaat atcacgtttc accgtacggg gtgtgacttc aaatttactg
     1681 accacctcct gtaccgtaac tactcctttt tctcgaa
//
LOCUS       DAOSZP010000512         1715 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_52022_length_1715_cov_2.635542, whole genome shotgun sequence.
ACCESSION   DAOSZP010000512 DAOSZP010000000
VERSION     DAOSZP010000512.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1715
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52022_length_1715_cov_2.635542"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1715
                     /locus_tag="PK348_09910"
     CDS             <1..>1715
                     /locus_tag="PK348_09910"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HXK66571.1"
                     /translation="AGMFDIDTSRRQIWRFLYEIPDDDFRKMFENVQKLDVSNVNIEK
                     FITFLHVYRMIFDKYNFSDIRAIEKLHQYAQENLFSPPENFFKRIEGNDDFDALEAIL
                     ELQHTLKSDILLSQQVFEPVDTIEFKRHIAFGIPSMYGSYKEKKFDTLKVFFHCNIVR
                     LLLFEKILENISIYPHQKVDYDAIKRVIKLFIQSFEIDGLANHEMRAVTSLLDAPNLT
                     LTQFRDVIYSLLVIHGEISDRFNDTFKSVSRIAIKNIGIDNIIHDFIPPDQPASIEVI
                     VDRFLRNRVMQSPLLQLLDNLLLKLKDNLIHELSYLGNVVILNKVDTRIHKGRLVHII
                     GKYSVQHDETELFAPLWEVGAKAQGLIIAANIDGINVPEGLVISSELYKRIKDGNINN
                     PRFKRKLIYMLKKYIDEFTGYRFGNPENPLLVSVRSGAVFSMPGVMDTITNVGMTEDI
                     VPYFAQYDEWFAWDCYRRVIHDFAISAFGMDRHIFENLMAQAKEEAGVDLKEKLNGKQ
                     MSLLTRKYRFAINKAGYSVPKDPYEQLFYAIIAVFQSWDSAIAQNYRRFINLSDDWGT
                     AVIVQR"
BASE COUNT          535 a          303 c          351 g          526 t
ORIGIN      
        1 gccggtatgt ttgatattga tacctcacgc aggcagatat ggcgattttt atatgagata
       61 ccggatgatg acttcaggaa gatgtttgaa aatgtacaga agttagatgt ttcaaatgtc
      121 aatatagaga aatttattac gttccttcat gtgtacagga tgatctttga taaatacaat
      181 ttttctgata tacgagccat agaaaagctt catcagtatg cacaggaaaa tctttttagt
      241 ccaccagaaa actttttcaa gcgaattgaa ggcaatgatg attttgacgc actcgaagcc
      301 attctggaac tgcaacatac gttaaaaagt gatattttgc tatcccaaca ggtatttgag
      361 ccagttgata ccattgagtt taaacgccat attgcgtttg gcataccgtc aatgtatggc
      421 tcatataaag aaaagaagtt tgatacactg aaagtgttct ttcactgcaa tatagtgagg
      481 ctcctccttt ttgaaaagat actggaaaat atcagcattt atccacatca aaaagttgac
      541 tatgatgcaa tcaaacgcgt tattaaattg ttcatacaat cttttgaaat tgatggcctt
      601 gcaaatcatg agatgagggc tgtgacaagc ctgcttgatg caccaaattt gacattaaca
      661 cagtttcgtg atgttattta tagcctgctg gttatccatg gtgaaatttc ggacaggttt
      721 aatgatacgt ttaaatcagt aagccgcatt gcaattaaaa atattggcat tgacaacata
      781 atccatgatt ttataccacc ggatcaacct gcaagtattg aagttattgt ggacaggttt
      841 ttacgtaaca gggtgatgca atccccattg ttgcagcttt tagacaatct attgctcaag
      901 ctaaaagaca atcttattca tgaactgtca tatttgggca atgtggttat tctcaataaa
      961 gtagatacac gcatacacaa aggcagactt gtccacataa ttgggaaata cagcgtgcaa
     1021 catgacgaaa ctgaactatt tgccccactg tgggaagttg gtgccaaagc tcaggggtta
     1081 attattgcgg caaatattga tggcattaat gtacctgaag gtcttgtcat ctcttcagaa
     1141 ttatataaac gtattaaaga tggcaatatc aataacccgc gctttaagcg aaagctcata
     1201 tatatgttaa aaaaatatat tgatgaattt actggctatc gatttggaaa tcctgaaaat
     1261 ccattacttg tctcagtgcg tagtggtgcg gtgttttcaa tgccaggtgt tatggatact
     1321 atcaccaatg tgggcatgac cgaggacatt gtaccctatt ttgcgcagta tgatgagtgg
     1381 tttgcgtggg attgttaccg acgtgtaatt catgattttg caatatcagc atttggtatg
     1441 gacaggcaca tctttgaaaa tcttatggca caggcaaaag aggaagctgg cgttgatctt
     1501 aaagaaaagc ttaacggcaa gcagatgtca ttgctaacac gcaaatatcg ctttgccatt
     1561 aataaagcag gttattctgt gcccaaagac ccctatgaac aattatttta tgcaatcatt
     1621 gcggtatttc aatcatggga ttcggccatt gcccagaact atcgccgctt tataaactta
     1681 tccgatgact ggggaacagc tgttattgta cagcg
//
LOCUS       DAOSZP010000513         1710 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_52208_length_1710_cov_3.273716, whole genome shotgun sequence.
ACCESSION   DAOSZP010000513 DAOSZP010000000
VERSION     DAOSZP010000513.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1710
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52208_length_1710_cov_3.273716"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            233..1507
                     /locus_tag="PK348_09915"
     CDS             233..1507
                     /locus_tag="PK348_09915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66572.1"
                     /translation="MNVKSNHCAYVLHGVFYFLLTIITQLYAQDVSQNEQLNFSLSGY
                     YKNLFIGYKTDEYFSEDYTQKEKLLVTDVNRVRLSPHCDIGQNIVFHADIDNEFIISN
                     YTQSKTFDATWIGFSYDQLYTDYYDYRNDTGYYRLKLHRAFAKCIIGKATITLGRQLV
                     RFGSGKIWNPLDIVNPISPLSFEGAEETKGVDAMRFELYPAHFLEVGFVYVPQRKNNE
                     FGDSFDNSVYLLRNKVVLSNIDFAILAGKIMQRNVAGLDVALPVYDGLLRGSFLVDKD
                     GDNTYYIAGTGYDYSFESGFSILLEYFYNGYCLTKNDKLYQAYYETFIYGMNEQRYRT
                     LAYHFLTYNRHYAGLSVSFSGASLFSASATSILDFEGHGVMLIPSVVYNIVEDCDLTF
                     YMLYAIVAGNNLSDFSSFDNRPALAAEVKYYF"
BASE COUNT          511 a          282 c          303 g          614 t
ORIGIN      
        1 tagcttagct ttttcaaaat cgcgtaaacc ctgctcgtac caatcgttcc atctgttcat
       61 gatttttcct gttaaataat attgtataca atttttcttg aattattatt atttcaatat
      121 atcaaatgaa gaaacaataa tcaactaata aaataatcta acgataatat gagttgatta
      181 acatactttt attttaacaa ttttttcaaa atcctatttt caagggaaaa acatgaacgt
      241 gaaaagcaat cattgtgctt atgtattaca tggcgtattt tattttttac ttactattat
      301 tactcagtta tatgcccagg atgtttccca aaacgaacaa ttaaattttt cactttctgg
      361 atattataaa aatcttttta ttggatacaa aactgatgaa tacttttctg aagattacac
      421 acaaaaagaa aagctgcttg taaccgatgt taacagagtt cgcctttcac cccattgtga
      481 tattggccag aatattgtat ttcatgctga tattgataat gaatttatca tatccaatta
      541 cacacaaagc aaaacatttg atgcaacatg gattggattt tcctatgatc aattatatac
      601 agattattat gattatcgca acgatactgg ttactatcgc ttaaaactgc accgtgcatt
      661 tgccaaatgt attattggga aagcaaccat tactttagga aggcaactgg tacgttttgg
      721 aagcgggaaa atatggaatc cacttgatat tgtaaaccca atatcaccac tttcatttga
      781 aggagcagaa gagacaaagg gggtggatgc catgcgtttt gagctgtacc ctgctcattt
      841 tttagaagtt gggtttgtct atgtacctca acgaaagaac aatgagtttg gagatagttt
      901 tgataattca gtgtaccttt tgcgcaacaa agtagttttg agcaatatag attttgcaat
      961 ccttgcaggg aagataatgc aaaggaatgt tgcaggttta gatgttgccc tgccagtata
     1021 tgatggcttg ttgcgtggaa gttttcttgt ggataaagat ggagataata cttactatat
     1081 tgcaggtaca ggctatgatt actcttttga aagtggtttc agtatcctgc tggagtattt
     1141 ttataatggc tactgtttga ctaaaaacga taaattgtat caggcatatt atgaaacgtt
     1201 tatctacggc atgaatgaac agcggtaccg tacgcttgca tatcatttcc tgacatataa
     1261 caggcattat gcagggttat cggtaagttt ttctggagct tcacttttca gcgccagtgc
     1321 aacatctatt cttgattttg aggggcatgg ggttatgctt ataccgtcag tagtgtataa
     1381 tatagtggaa gattgtgatc tgacgttcta tatgttatat gcgatagttg ctggcaacaa
     1441 tctttcagat ttttcctcat ttgacaatag gcctgcactt gcagcagaag ttaaatatta
     1501 tttttaatat gtattgtaaa tgccttttat tgtagctgat cttctaccac cagtaccagt
     1561 gtcctgtatg cttcattctt gcttattcaa gttctgtagc aggatattca tttttatcaa
     1621 aagatctatc ctctcatgag ttctattttc tttaaacact gcttccttta cctctttgtt
     1681 tactcctgct actatgactt tttgtgattg
//
LOCUS       DAOSZP010000514         1708 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_52278_length_1708_cov_4.128857, whole genome shotgun sequence.
ACCESSION   DAOSZP010000514 DAOSZP010000000
VERSION     DAOSZP010000514.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1708
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52278_length_1708_cov_4.128857"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1708)
                     /locus_tag="PK348_09920"
     CDS             complement(<1..>1708)
                     /locus_tag="PK348_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016777118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HXK66573.1"
                     /translation="IEKTYGRKGADVVSKNMAAVDEGAKNVKEVKVPEEWKNIELPGK
                     PKFEELPEFIREIVEPMNRLEGDSLPVSAFLPRPDGTFPPGTTQYEKRGIAVNVPEWQ
                     PDNCIQCNQCAYVCPHAVIRPFLLNEDEMKKAPADMVTLDAKGKGLEGLKFRIQISPL
                     DCTGCGNCAEECPAKEKALIMKPLETQLNQQKYWDFIVKNVTYKDNLMNKYTVKGSQF
                     CQPLFEFSGACAGCGETPYIKLITQLFGDRMLVANATGCSSIYGGSAPATPYCINAQG
                     RGPAWASSLFEDASEFGFGIHLGVAQQRNKLAQLLDQLAKTTSNSQLKDAALEWLAGK
                     DNAEKSREATKKLLPLLKGDSSKEAAEVLKLAQYLEKKSIWVFGGDGFAYDIGFGGLD
                     HVIASGEDVNILVLDTEVYSNTGGQSSKATPIAAQAKFAAAGKRVRKKDLGLMAITYG
                     YVYVAQVAMGASQMQYMRALTEAESYPGPSLIIAYSPCINHGLREGMGKAQEEEKRAV
                     ECGYWSLWRYNPLLEKEGKNPFILDSKEPQWDKFNDFLMGEVRYSALKKEFPEVAEQL
                     FAA"
BASE COUNT          453 a          414 c          298 g          543 t
ORIGIN      
        1 cagctgcaaa taattgttct gcaacttcag gaaattcctt ctttaatgct gaatagcgta
       61 cctcacccat gaggaagtca ttaaatttat cccactgtgg ttctttggaa tcaaggataa
      121 atggattttt cccttctttt tcaagtaatg gattgtaccg ccataatgac cagtatccac
      181 attcaactgc cctcttttcc tcttcctgag cttttcccat accttcgcgc aatccatgat
      241 taatacatgg cgaatatgca ataataagtg atggaccagg gtagctttca gcttcggtta
      301 atgcgcgcat atactgcatc tggcttgcac ccatggcaac ctgagctaca tacacatatc
      361 catacgttat tgccatgagc cccaggtctt ttttacgtac ccgtttgcca gctgcagcaa
      421 atttagcctg tgctgctatt ggtgttgctt ttgatgattg accaccggta ttggaatata
      481 cttcagtgtc taacaccagt atatttacat cttcacctga agcaataacg tgatccagac
      541 caccgaaccc aatgtcatat gcaaacccat caccaccaaa cacccagatt gatttctttt
      601 ctaagtactg tgccaatttc agcacttcag ctgcttcttt agagctatcg ccctttaaaa
      661 gcggtaataa tttcttagtt gcttcacgtg atttttcagc gttgtcttta cctgcaagcc
      721 actcaagcgc cgcatcttta agctgggaat tacttgttgt ttttgccagc tgatctaaaa
      781 gctgtgcaag tttattgcgc tgttgagcaa cacctaaatg tattccgaaa ccaaattcgc
      841 tggcatcttc aaaaagtgaa cttgcccatg caggtccacg tccctgtgca ttgatacaat
      901 acggtgtagc aggagctgaa ccaccataaa ttgatgaaca gcctgttgcg ttagcgacaa
      961 gcatcctgtc accaaaaagc tgtgtaataa gcttgatgta aggagtttca ccacaacctg
     1021 cacatgcgcc agagaactca aacaatggct ggcagaattg gctccccttt acagtgtatt
     1081 tattcatcaa attatcttta taggtaacat tttttactat aaagtcccag tatttctgct
     1141 gattcaactg ggtttcaagt ggtttcataa ttaatgcttt ttcttttgca ggacactctt
     1201 cagcacagtt tccacaaccc gtacaatcta aaggacttat ttgaattctg aatttaagcc
     1261 cttcaagtcc tttgcctttt gcatcaagcg ttaccatatc ggcaggagct tttttcattt
     1321 catcttcatt cagcaggaat gggcgtatca ctgcatgtgg acacacatat gcgcactgat
     1381 tacactgaat acaattatca ggctgccatt cgggaacatt gacggcaata cctcgctttt
     1441 catactgtgt ggtacctgga gggaatgtac catcaggtct tggcaaaaag gcacttactg
     1501 gtaaactatc gccctctaac ctgttcattg gttcaacaat ctcacgaata aactcaggca
     1561 attcttcaaa ttttggctta ccaggaagtt caatattttt ccactcttca ggaactttca
     1621 cttcttttac attctttgca ccttcatcaa ctgctgccat atttttgctt acaacatcag
     1681 caccttttct gccataagtt ttttcaat
//
LOCUS       DAOSZP010000515         1700 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_52564_length_1700_cov_3.254711, whole genome shotgun sequence.
ACCESSION   DAOSZP010000515 DAOSZP010000000
VERSION     DAOSZP010000515.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1700
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52564_length_1700_cov_3.254711"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1117
                     /locus_tag="PK348_09925"
     CDS             <1..1117
                     /locus_tag="PK348_09925"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HXK66574.1"
                     /translation="QLNLLEKFDITRGGVVAKLAHDYGTYSGSLQAEGNFGHHESTLE
                     STGADKFYSNDSSYAVRLKEVTKLWQGNTLTAGAEWRRYGGTAKDRSSGTYYLDDKFM
                     YDTSVYALMDQNLFKDVLSINGGARYSYNSEYGDYTAWQAGAAVRPLEKTKMYTNVAK
                     GFKFPDIRQVYLQGMFPAINPNPDLKPETYTSAEIGLEQQIIEKINIHVAGYRIWSKD
                     KFVYTTQWINAPDFKYNGAEASVTVQALTWLAFNAGYSYIDNEQDDTYLPYVPKHKAN
                     AGLTVSYKDFKAGIDGEYIKGLYADIAGVKEFDSYLVANASLSYTILEKYTAFVNFYN
                     ITDKDYATFAVSNPTQTLFWEYPMPGFHFLAGLKATF"
     gene            1279..>1700
                     /gene="amrB"
                     /locus_tag="PK348_09930"
     CDS             1279..>1700
                     /gene="amrB"
                     /locus_tag="PK348_09930"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HXK66575.1"
                     /translation="MWQRKPGFAGSFYPASPEKLKNEIITYLSKATVKKLEHPVAFIV
                     PHAGYIYSGPVAAYSYAQLKENPADVVIVLAPSHRARFNGASIITNGIFSTPLGDITV
                     DSTIAKRLLDEVVFFEIKEAHQYEHSLEVQLPFLQVVL"
BASE COUNT          555 a          293 c          341 g          511 t
ORIGIN      
        1 ccaattaaat cttttagaaa agtttgatat tactcgtggt ggtgttgtgg ccaaattagc
       61 ccatgattat ggtacctaca gtggttcgct acaggcagaa ggcaactttg gtcatcatga
      121 atcaacactg gagtcaacag gagctgataa attttactcc aatgacagtt cttatgcagt
      181 cagattaaaa gaagttacta aactatggca ggggaatact ttaacagcag gtgctgaatg
      241 gcggcggtat ggtggtacag caaaggacag gtcatcaggc acttattatt tagatgataa
      301 attcatgtat gataccagtg tgtatgccct gatggatcaa aatcttttta aggatgttct
      361 gagcattaac ggtggtgcac gatattccta taattcagag tatggcgatt atacagcctg
      421 gcaggcaggt gcagcagttc gtccactgga aaagactaaa atgtatacca atgtggcaaa
      481 aggatttaaa ttccccgata tacgacaggt gtatttacaa ggaatgttcc ctgctataaa
      541 ccccaatcca gatttaaaac ctgaaacata tacgtcagca gaaataggac tggaacaaca
      601 gattattgag aaaattaata tacatgttgc aggatataga atatggtcta aggataaatt
      661 tgtttataca actcaatgga taaatgctcc agattttaaa tacaatggag ctgaagcgtc
      721 ggttaccgtg caagcattaa cgtggttagc gttcaatgca ggatattcgt atattgataa
      781 tgaacaggat gacacctatt taccctatgt accaaaacat aaagcaaatg caggcctgac
      841 agtaagctac aaggatttta aagctggaat agatggcgaa tatataaaag gattatatgc
      901 tgatatagcc ggagtaaaag aatttgacag ttacctggtt gcaaatgcat cgctgtcata
      961 tacaatcctg gaaaaataca cagcatttgt taatttctat aatattactg ataaagacta
     1021 tgctacattt gctgtatcaa atccaacaca aacactattt tgggaatatc caatgccagg
     1081 ttttcatttc ttagcagggt tgaaggcaac gttctaagct caatactgca gtattaccta
     1141 ttgtatcaga ttaatagtag taacacatta aaggtgtgca ttatgcacac ctttttttat
     1201 aatttgccag aataaaatca cttgcaaaga ggggtatgtt ttattatatt taatataatc
     1261 attcgtggag gttttagtat gtggcaacgc aaacccggat ttgcaggttc tttttatccg
     1321 gcaagtcctg aaaaattaaa aaatgaaatc attacctact tatcaaaagc aactgtaaaa
     1381 aagctggaac atcctgtagc atttatagta cctcatgcag ggtatatcta ttctggcccg
     1441 gtagctgcat attcatatgc acagctaaaa gaaaatcctg ctgatgttgt aattgtactg
     1501 gcaccttctc accgtgcacg atttaacggt gcttcaatta ttaccaatgg tattttcagt
     1561 acccctcttg gtgatattac cgtggatagt accattgcta aaaggcttct tgatgaggta
     1621 gttttttttg agattaaaga agcacatcaa tatgaacatt cgctcgaggt acaacttcct
     1681 tttttgcagg tagttttgaa
//
LOCUS       DAOSZP010000516         1696 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_52741_length_1696_cov_3.536258, whole genome shotgun sequence.
ACCESSION   DAOSZP010000516 DAOSZP010000000
VERSION     DAOSZP010000516.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1696
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52741_length_1696_cov_3.536258"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            54..1073
                     /locus_tag="PK348_09935"
     CDS             54..1073
                     /locus_tag="PK348_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese transporter"
                     /protein_id="HXK66576.1"
                     /translation="MHSFIAIIKEASFVSVFVIVMMLLIEYINVVTQGKIANLKSTRL
                     KQYTIAVILGIIPGCLGGFASAAMYAHGAISLGAIVATMIATSGDEAFVMFALFPAVA
                     FALHIGLALIGIVAGYCTDLIFKKHGPVEHCCDGLVIHDEIPFSHYTPGNIISLWKNL
                     SLSRASLMSLLLVALLMVITGFFHQEEGFVKIALPVIISIAIIIVAIVPEHFLQEHLW
                     EHVIKKHLIRIFLWTLSALIVIHLLVDVLHLDALIHNAQWIVLVIAALVGIIPESGPH
                     LLFVMMFAKGMVPLSVLVTSSIVQDGHAMLPLLAQSRKDFLLIKCINLIVGLIVGSII
                     MLTGN"
     gene            1130..>1696
                     /locus_tag="PK348_09940"
     CDS             1130..>1696
                     /locus_tag="PK348_09940"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HXK66577.1"
                     /translation="MKNILVTGGAGYIGSHVTRLLAKKGFNPIVYDNLSGGFKDFVSG
                     FEFIKGDIGDFDKLAHVLSHYSIQCVMNFASYIAVGESVQYPLKYYENNVAKTLTLFE
                     AMIKTNVKRFIFSSSAAVYGNPEIIPIPEESPMKPESPYGKTKYFIEEVLRDLSVSNS
                     FKAISLRYFNAAGADESGQIGESHVPETH"
BASE COUNT          489 a          266 c          348 g          593 t
ORIGIN      
        1 tattcagtgt aatgtaaatt gtataaatag aatttcaggg ggtaataatc cttatgcatt
       61 cctttattgc aattattaaa gaagcatcat ttgttagcgt ttttgttatt gtgatgatgc
      121 tgcttataga atatattaac gtggttacac agggcaagat tgccaacctt aaatcaacaa
      181 gattaaaaca atatactatt gctgttattc tgggtattat tccaggctgt ctgggtggtt
      241 ttgcatctgc agctatgtat gcacatggtg caatatcact gggtgcaatt gttgccacaa
      301 tgatagcaac cagtggtgat gaagcatttg ttatgtttgc tcttttccct gcagtggcat
      361 ttgcattaca tattggcctt gctttgattg gcattgtggc aggatattgc acagatttga
      421 ttttcaaaaa acatggtcca gttgaacatt gctgtgatgg gctggtaatt catgatgaaa
      481 ttcctttttc acattacacg cctggcaata ttatttcatt atggaaaaat ctttctttgt
      541 cgcgggcttc gctgatgtca ttgctgctag ttgctttatt aatggttata actggtttct
      601 tccaccagga ggaaggtttt gtaaaaattg cattgccagt tattattagt atagccatta
      661 ttattgtggc gatagtccct gaacattttt tacaagagca tttatgggag catgttataa
      721 aaaaacatct tataagaata tttttgtgga cactatcggc gctgattgtc attcatctgc
      781 tggtagatgt gttgcattta gatgcattaa ttcataatgc ccaatggatt gttcttgtta
      841 ttgctgcgct tgttggtata atacccgaat caggcccaca tcttttgttc gtaatgatgt
      901 ttgcaaaagg gatggtgcca ctttcagtgc tggtaacaag ttctatagtg caggatggcc
      961 atgccatgct gccactttta gcacaatcaa gaaaggattt tttattgata aaatgtatta
     1021 atcttatagt gggtttgata gtaggttcta tcattatgtt gacaggtaat tgaaaagttt
     1081 attttggtgt caaacgtgaa taattaatat ttttggtgga gatattttta tgaaaaatat
     1141 tttagtcacc ggtggtgcag gttatattgg tagtcatgtc acacgattgc ttgcaaaaaa
     1201 aggatttaat cctatcgtat atgataattt aagcggtgga tttaaagatt ttgtttcggg
     1261 ttttgaattt attaaaggcg acattggcga ctttgataaa cttgcacatg tactatcgca
     1321 ctatagtatt cagtgcgtta tgaattttgc ttcatacatt gccgtaggag aatcagttca
     1381 gtacccactg aaatattacg aaaacaatgt ggcaaaaaca ttaaccctct ttgaagcaat
     1441 gataaagaca aatgtaaaac gattcatatt ctcatcctct gcagcggtgt atggtaatcc
     1501 ggaaataatt ccaatacctg aagaaagtcc catgaaacct gaaagcccat atggtaaaac
     1561 caagtatttc attgaagaag ttttacgtga tctttcagta tctaattctt ttaaggcaat
     1621 cagtttacgc tattttaacg ctgcaggtgc agatgagtca ggtcagattg gcgaaagcca
     1681 tgtgccagaa acacat
//
LOCUS       DAOSZP010000517         1688 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_53059_length_1688_cov_3.903246, whole genome shotgun sequence.
ACCESSION   DAOSZP010000517 DAOSZP010000000
VERSION     DAOSZP010000517.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1688
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53059_length_1688_cov_3.903246"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            135..1490
                     /locus_tag="PK348_09945"
     CDS             135..1490
                     /locus_tag="PK348_09945"
                     /inference="COORDINATES: protein motif:HMM:NF016362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXK66578.1"
                     /translation="MSLLSITFFIVGASIVGALLLVIRAHQYPAKVRKAQEYLDSGDI
                     AKASEIVKLVLERKKDYVPARYLRAQILVKQKQYLLAISELNGILALPEFNKHVNELD
                     IHYMLADLYHETQQWQKEVEEFRIILQFNPDDINANHRLGIVLYRQHDYIGAKDYLLK
                     AVTMDPALTDIYAPLGICLFNLADYELAQNFLIKAIESNPSNYEPYYYLGMIYKMKKD
                     YVAALDMLSRARNDRRYFVKSQYAIGQIYFDNDEYSKAIEELEKGLSYVKDSTDEGLA
                     YRYLLAECYEMENKIQEAVHHWEKIASINPNYRSTQLKLDDYKAILENKNLKFLFTSS
                     MSELQPLITEIIAQLNYSIVYKKEVAPNEYMYKCFSVKRINEPPALINFIRTTRDITE
                     GQINAFYQRMLEEKCRSGIFITTSKFSINAKSQAANKMIEIMDGRFLQKTMERIKGKK
                     V"
BASE COUNT          579 a          271 c          331 g          507 t
ORIGIN      
        1 cgataattcc tacttctgcc attgttatgt tcctttcttt ttaattattt attataagct
       61 aaggcacact ataatgcttg acttaaaata taatgcgtgc agtaataaaa tcaaatttta
      121 aatatgaaaa ttgtatgtca ctgctaagta taacattttt tattgtgggt gcatctattg
      181 ttggtgcatt gcttttagtt atacgtgctc accagtatcc tgcaaaggtg cgtaaagctc
      241 aggaatacct tgatagtgga gacatcgcaa aggcaagtga aattgtcaag cttgttcttg
      301 aacgaaaaaa agattatgtt cccgcacggt acttaagggc acagatttta gtaaaacaaa
      361 aacaatatct tttagcaatt tccgaactca atggtattct tgcccttcct gaattcaata
      421 agcatgttaa tgaacttgat atccattata tgcttgccga cctgtaccat gaaacacagc
      481 aatggcaaaa ggaggttgaa gagttcagga taattttaca gtttaatcct gatgatatta
      541 atgcaaacca ccgacttggt atagtgttat accgacaaca tgattatatt ggtgcaaaag
      601 attatttgct taaagcagtt accatggatc ctgctctgac agatatttat gcaccgctgg
      661 gaatatgcct ttttaacctg gcagattatg aactggcgca aaactttctc ataaaggcaa
      721 ttgaaagcaa tccttcaaat tatgaaccat attattatct gggtatgatt tataaaatga
      781 aaaaggacta tgtggcagcg ctggacatgc tatcaagggc gcgcaatgac aggcgatatt
      841 ttgtgaaaag ccagtatgct attggccaga tttattttga caatgatgaa tacagtaagg
      901 ctattgagga attggagaag ggattatcct atgtaaagga tagtactgat gaaggactgg
      961 cttaccggta tttgctggct gagtgttatg aaatggaaaa taagattcag gaggctgtac
     1021 atcactggga aaaaattgca agcataaatc caaattaccg cagtactcag cttaagctgg
     1081 atgactacaa agcaatactg gaaaataaaa atttgaaatt tttgtttact tcatcaatgt
     1141 ctgagttgca gcccctaata acagaaataa ttgcccagct taattacagc attgtataca
     1201 aaaaagaggt tgcaccaaac gagtatatgt ataaatgctt cagtgtaaaa agaataaatg
     1261 aacctccagc attaatcaac ttcatacgaa caacccgtga tataactgaa ggccagatta
     1321 atgcctttta tcaaagaatg ctggaagaga aatgcagaag cggcatattt attacgactt
     1381 caaaattcag cataaatgct aaatcacagg cagcaaataa aatgattgaa attatggatg
     1441 gaagattttt acagaaaacc atggaaagga ttaaggggaa aaaggtttaa taatgaacag
     1501 gtttttgtat ttgctgatgt gtatgctgtt gataacagtt tcctgttctc gcgaaaagga
     1561 aatcaatgaa gggatacggc tttacaatga aggtcttacg caaagtgcat ttgtaaaatt
     1621 cagggaagga ttgctacagt attctgctat taagactatc aatgctcaga atctttattt
     1681 tacacctt
//
LOCUS       DAOSZP010000518         1687 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_53100_length_1687_cov_3.748775, whole genome shotgun sequence.
ACCESSION   DAOSZP010000518 DAOSZP010000000
VERSION     DAOSZP010000518.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1687
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53100_length_1687_cov_3.748775"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(85..1122)
                     /gene="mtnA"
                     /locus_tag="PK348_09950"
     CDS             complement(85..1122)
                     /gene="mtnA"
                     /locus_tag="PK348_09950"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HXK66579.1"
                     /translation="MMYYTIRWDESAVSMLDQRMLPVQEVYRHYTNYHDVADAIRSMV
                     VRGAPAIGIAAAMGIALAAVKSKTNNINDFKSEMLQVCQIFASTRPTAVNLFWAINRM
                     RNIIEKADNIDMCKNDLVREAQKIHQEDIEINKKIGEFGQKYIEDGITVMTYCNAGAL
                     ATGGYGTALGVIRSAVEHGKKIKVYACETRPYLQGARITAWELFKDGIDTTLICDNMA
                     GHFMKKGEIHCVIVGADRIAANGDTANKIGTYTMSILAKAHGIPFYVAAPISTLDPDT
                     PDGNSIVIEERNIDEVAYIQGVQIAPEGIFIKNPAFDVTPAANITAIITEKGVIENPV
                     ATGIAPLVNVR"
     gene            complement(1094..>1687)
                     /locus_tag="PK348_09955"
     CDS             complement(1094..>1687)
                     /locus_tag="PK348_09955"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXK66580.1"
                     /translation="IVRNILRRKQQHSLENRLGEYIIHDQRTLVIPVDETIADIVAYE
                     LTKHLPYAGFCNRSTAENISMALLEALSNAMYHGNLEIPSEVREEQGMKGFNDEIQKR
                     LQDPQFNNRKVTIHYHYTGDYVEYIIEDEGKGFDYTNVADPRDPENFLKNSGRGLFII
                     QVHMDEVAWEKGGRQIRMRKYRTIQRGGNNVLYHTLG"
BASE COUNT          450 a          372 c          279 g          586 t
ORIGIN      
        1 gagtattacc gaaagaagta ttccatgata cttgggtggg tgtaaaaaaa aataagaaaa
       61 ggggtcagag cttaataaca gtaactatcg cacatttaca agtggtgcaa tacctgttgc
      121 aaccggattt tcaataacac ctttttctgt aataatggcc gtaatgtttg cagcaggagt
      181 aacatcaaat gctggatttt ttatgaatat tccttctgga gctatctgta ctccctgaat
      241 atacgcaact tcatcaatat ttcgttcttc aatgacaatg ctgttgccat caggtgtatc
      301 aggatccagt gttgatatag gggctgccac ataaaaaggt atcccatgtg cttttgccag
      361 tatagacatt gtgtatgttc caatcttgtt tgcagtgtca ccatttgctg caatcctgtc
      421 tgctccaaca atgacacaat ggatttcacc tttcttcatg aaatgaccag ccatattatc
      481 acagataaga gtggtgtcta taccatcttt gaaaagttcc catgcagtga tccgtgcacc
      541 ctgcaagtac ggtcttgttt cgcatgcata tacttttatt ttctttccat gctcaacagc
      601 acttctaatt actcccagtg ccgtaccata tccaccagta gccagtgctc ctgcattaca
      661 gtaggtcatt actgtaatgc catcctcaat atatttttga ccaaattccc caatcttttt
      721 atttatttca atatcttcct ggtgaatttt ttgtgcttct cgcaccagat catttttaca
      781 catatcaatg ttgtctgctt tttcaattat attcctcatc ctgttgattg cccagaaaag
      841 attaacagca gttggccgtg ttgatgcaaa aatctggcat acctgcagca tttcagattt
      901 aaaatcatta atattgtttg ttttactctt aacagccgcc agtgctatac ccatagctgc
      961 agctataccg atagccggag ctcctctgac taccattgac cgtattgcat ctgctacatc
     1021 atgatagttt gtgtaatgtc tgtatacctc ctgtactggt aacatgcgtt gatccaacat
     1081 tgaaactgct gattcatccc agcgtatggt atagtacatt attccctcct cgctgtattg
     1141 tcctgtattt tctcattcgt atctgtcttc cacctttttc ccatgcaact tcatccatgt
     1201 ggacctgaat tataaaaaga ccgcgcccac tgttttttaa aaaattttcc ggatctcttg
     1261 ggtctgcaac attggtataa tcaaatccct tgccttcatc ttcaattata tattcaacat
     1321 aatcaccagt ataatgataa tgaatagtta cttttctgtt attaaactga gggtcctgta
     1381 gccgcttctg tatttcatca ttaaaccctt tcattccctg ttcttctctt acttcagatg
     1441 gtatttccag atttccatga tacatggcat tggaaagtgc ttctaaaaga gccatggaaa
     1501 tattttcagc agttgaacga ttgcaaaatc ctgcatatgg cagatgtttt gttaattcat
     1561 atgcaacaat atctgctata gtttcatcaa caggtatgac cagtgttctt tgatcatgta
     1621 taatatattc tcctaaccgg ttttccaggc tatgctgctg tttcctgcgt aaaatgtttc
     1681 ttacaat
//
LOCUS       DAOSZP010000519         1678 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_53506_length_1678_cov_2.704868, whole genome shotgun sequence.
ACCESSION   DAOSZP010000519 DAOSZP010000000
VERSION     DAOSZP010000519.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1678
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53506_length_1678_cov_2.704868"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            255..932
                     /locus_tag="PK348_09960"
     CDS             255..932
                     /locus_tag="PK348_09960"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HXK66581.1"
                     /translation="MYTIHIFFLFAFGLFIGFISSIPVGAVQLEVIKKAINGHLKPAI
                     SVALGSVTSDFIYGVLVLFGIGGFLHYPKVQIFTYSLGILVIIALLYRSYREHKHIPP
                     SHKPHIQYKKRTSYLTGLTIAITNPGMIVWWVIGYKLLLDLQLFDTMNIPLKATFLVS
                     CCTGLAGYLTLIAFMMNKLQESISDRLLDRMNIVIMILLVFVIIYFSFKLYGLLTNTT
                     IDILYNV"
     gene            1012..1458
                     /locus_tag="PK348_09965"
     CDS             1012..1458
                     /locus_tag="PK348_09965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66582.1"
                     /translation="MDIIATQQFIDTLRKLPDHSTLDREFFAPLYDFFEDAGASLLLS
                     TPDDYYLLYFDLPEAIDDILPTNVSWLVEKDEKTISLTMFADGKEIQTYHTFHFANNP
                     VNAYFFKSLQDSRTLTINFFAMMYGDIYKLKSIDFTLPQAILQQIE"
     gene            1463..>1678
                     /locus_tag="PK348_09970"
     CDS             1463..>1678
                     /locus_tag="PK348_09970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66583.1"
                     /translation="MQEKAKEYIEYIKKGEMPPDNNQRKKKFSRIFLLINIIVFIILL
                     TLIIQKKEPTDYIYQTVAVNGVQVRFSI"
BASE COUNT          515 a          343 c          264 g          556 t
ORIGIN      
        1 ggctttgctt caaagaattg tatccctgtt attgacaacc tcaaagttta caaaggcaat
       61 gacatcatct ttcaggacga tttttcaaaa gaatcaatac gcaaaatagt gctgcagggc
      121 aaaaaactta caccccagga agttgaagaa tataaaaaga aaaacaataa ataatattta
      181 caaaaaatac ttgcaacttt ttactgcaat ttgcatcgta tataatacgc attttattac
      241 gtgtagggta tggcatgtac accattcata tctttttttt atttgcattt ggccttttta
      301 ttggatttat ttcttcaatc cctgttggcg ctgtccagct tgaagtcatt aaaaaagcca
      361 ttaacggcca tttaaaacca gctatatcgg tagcattggg ctcggttacc tctgatttta
      421 tttacggagt tcttgtgctt tttggcattg gtggtttttt gcattaccca aaagttcaga
      481 tctttaccta ttcacttggt attcttgtca tcatagcgct tttgtaccgt tcataccgtg
      541 agcacaaaca cataccgcct tcacacaaac cccacattca gtataaaaaa cgcacatcat
      601 atcttacggg attaaccatt gccattacca atcccggcat gattgtgtgg tgggtcattg
      661 gctataagct tcttttagat ttgcaactgt ttgacaccat gaacattccc ctcaaagcca
      721 cctttctggt atcctgctgt acaggacttg ccggttacct tacactgatt gcattcatga
      781 tgaataaatt gcaggaatca atctccgaca gattgcttga cagaatgaat attgtcataa
      841 tgattttact tgtgtttgtt attatctatt tttcattcaa gctgtatggg ttacttacca
      901 acaccactat tgacattctg tataatgttt aggcgatagt tactgtcaat atacactaac
      961 gtctgcacag gctttttact gtaaattttt tttgggcgat agttactgaa tatggacatc
     1021 attgcaacac aacagtttat tgacacatta cgaaaacttc ctgaccactc tactcttgac
     1081 agagagttct ttgccccgct atatgatttt tttgaagatg caggcgcatc actgctattg
     1141 tcaacacctg atgattatta ccttttatat tttgatttac ctgaagccat tgacgatatt
     1201 ttgcctacca atgtttcatg gcttgtagaa aaagatgaaa aaacaatttc actcaccatg
     1261 tttgctgatg gtaaggaaat tcaaacatac catacattcc attttgctaa taatcctgta
     1321 aatgcctatt tcttcaaatc attacaggat agcaggacat taacaattaa tttttttgcc
     1381 atgatgtatg gcgatatcta caaactaaaa tccatcgatt ttactcttcc tcaagctata
     1441 ctacagcaga tagaataaca caatgcagga aaaagcaaag gaatacattg aatatatcaa
     1501 aaaaggtgaa atgcctcctg acaacaatca gcgcaaaaag aaattttcac gcatcttttt
     1561 gctgattaac attatagtct tcatcatcct cttaacactc atcatccaga aaaaagagcc
     1621 aactgattat atctatcaaa ccgttgccgt aaacggtgtt caggtgcgct tttccatt
//
LOCUS       DAOSZP010000520         1676 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_53592_length_1676_cov_3.045034, whole genome shotgun sequence.
ACCESSION   DAOSZP010000520 DAOSZP010000000
VERSION     DAOSZP010000520.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53592_length_1676_cov_3.045034"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(89..1198)
                     /locus_tag="PK348_09975"
     CDS             complement(89..1198)
                     /locus_tag="PK348_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015903321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S double cluster binding domain-containing
                     protein"
                     /protein_id="HXK66584.1"
                     /translation="MLSKQEIIDKARELQFDDIGFATAEPFEEHKQFLTTVPDNFTWA
                     RVFGLPLEEGCNPQSILPQAKTIIVLIENYFKFSFPKSMEPYFGRCYMDDDRVTKDGL
                     TVRIRQFVSFLREHGLQAKVTFSIPQKAASARAGIGNFGKNCLLYANNAAGKSSWISP
                     ITILVDKAFEADEPTVRVGCPDWCRNACVAACPTRALKGNCTIDSKRCISFLTYFGQG
                     ITPRELREPMGTYIYGCDRCQDVCPRNRAWMSQVLPLNPRVAAKEPWFDIVKLLHMDD
                     AYFQHNIQPHMFYMPASDLWRWKMNVARAMGNSLNERFVPELIKAYEDNSDDRVRGMI
                     AWSLGRIGGAKAKAALERWRHSATPAVKEEIEMAL"
     gene            complement(1173..>1676)
                     /locus_tag="PK348_09980"
     CDS             complement(1173..>1676)
                     /locus_tag="PK348_09980"
                     /inference="COORDINATES: protein motif:HMM:NF028235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HXK66585.1"
                     /translation="YFKNKEELILEALKEAHRQYQEYLAAYTAKVSEPLHKIDAMLDA
                     VYTYHKKRKFRGGCIFGNIALEMADKNIAYRGFIHDVFQQWIDWTATQLEAAVAGGQL
                     LASTPVQELSHHIIAALEGGLLLSKVTKNPDDLNAAIRGIKELLHYYKTNKGLKRRNN
                     AIKTGNN"
BASE COUNT          458 a          385 c          304 g          529 t
ORIGIN      
        1 ttataaaata ttaaatagat ttcaatagat agatagttaa tttataaaat gaaataagtt
       61 ctaagttttt ctattctacg atatatatct acaacgccat ctcaatttct tctttcactg
      121 ctggtgtagc gctatgtcgc cagcgttcaa gtgcagcttt tgcttttgcg ccaccaattc
      181 ttcccagtga ccaggcaatc attcctctca cacgatcatc gctgttgtct tcataagctt
      241 taataagttc tggtacaaaa cgctcattga gactattacc cattgcccgt gctacattca
      301 tcttccatcg ccacaaatcg cttgcaggca tgtaaaacat atgtggctga atattgtgtt
      361 gaaaataggc atcatccata tgaagcagct ttacaatatc aaaccatggt tcctttgcag
      421 ccacgcgcgg gtttagtggc agcacctgtg acatccatgc tctgttgcgt ggacatacat
      481 cctgacaccg atcacaacca tatatatacg tgcccatggg ttctcgaagt tctcgaggcg
      541 taataccctg cccaaaatat gtaagaaatg aaatacaccg cttggagtca atagtacagt
      601 tgcccttcaa cgcccgtgtg ggacatgcag ctacacaggc attacgacac caatcaggac
      661 atcccactct tactgttggt tcatctgctt caaatgcttt atcaaccagt atggtgatgg
      721 ggcttatcca tgaactcttg cccgctgcat tgtttgcata cagtaaacag ttttttccaa
      781 aatttccaat gcctgccctg gctgaagcag ccttttgtgg aatggaaaac gtcacttttg
      841 cctgaagacc atgttcacgc aaaaaggata caaactgccg tattctgacc gtcaacccat
      901 ccttggttac acggtcatca tccatatagc aacgtccaaa gtatggttcc attgatttag
      961 gaaacgaaaa tttaaaatag ttttcaatga gcacaataat agtctttgcc tgcggaagta
     1021 tgctttgagg attacagcct tcttcaagtg gcagaccaaa gacacgtgcc catgtaaaat
     1081 tgtcaggtac tgttgttaaa aactgtttgt gttcttcaaa tggctctgct gttgcaaacc
     1141 caatatcatc aaattgcagc tctcttgcct tatcaattat ttcctgtttt gatagcatta
     1201 ttcctccgtt ttaaaccttt attggtttta tagtaatgta ataactcttt tataccccga
     1261 atagcagcat tcaggtcatc aggatttttt gtaacttttg atagaaggag ccctccctca
     1321 agtgcagcaa taatatggtg cgatagttcc tgtaccggtg tactggccaa caattgccca
     1381 cctgccacag cagcctccag ctgtgttgca gtccagtcaa tccattgctg aaaaacatca
     1441 tggatgaatc ctcgataggc tatattttta tcagccattt ccagtgcgat attcccaaag
     1501 atgcacccgc ctctaaattt tcgttttttg tggtatgtgt atactgcatc aagcattgca
     1561 tctattttgt gtagaggctc agaaaccttt gcagtatatg ctgcaagata ttcctgatac
     1621 tgccgatgag cttctttgag tgcttctaaa attagttctt ctttattttt aaaata
//
LOCUS       DAOSZP010000521         1674 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_53659_length_1674_cov_4.038913, whole genome shotgun sequence.
ACCESSION   DAOSZP010000521 DAOSZP010000000
VERSION     DAOSZP010000521.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1674)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1674)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1674
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53659_length_1674_cov_4.038913"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            186..1142
                     /locus_tag="PK348_09985"
     CDS             186..1142
                     /locus_tag="PK348_09985"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014585832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HXK66586.1"
                     /translation="MNILILGGGAVGLGLARSLITAGNDVTIIDVEPTVSALKEKGIM
                     QTGVLGEFTIQPGIFKVYDSLAKIGNVKIDYICVCIKSYLSETIAQVLKQYEHIIGTC
                     PIVLFQNGWGNAEQFIKYFEKDTIFNARVMTGYIKPALHRVDITVHADAIHIGSLYGG
                     DITQLLSLASELTKGGMPAEVTEHVSKDIWAKMLYNCALNPLGAIFNVPYGELGKSEY
                     TKAIMNDIFHEIFETMHKAGYSTHYRSAEEYIDVFYSRLLPSTGEHRSSMLQDIQAKR
                     RTEIDAMNGAVVALAKQHGVDTPVNEVITGLIKFIEGGYHGK"
     gene            1132..>1674
                     /locus_tag="PK348_09990"
     CDS             1132..>1674
                     /locus_tag="PK348_09990"
                     /inference="COORDINATES: protein motif:HMM:NF024221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HXK66587.1"
                     /translation="MANSLVRSGNPVLRSDPFTKEIGEPATDVMTIEGAVNKTIILLL
                     IVVGFATLSWYHIENLLFNNYLHMFLIVLSILGFIVALLTIFMKKLSPWTAPVYSAIQ
                     GLFLGIVSMLFEKSFPGIVLQAVGLTFGILFSLLLAYKSGLIKATENFKLGVAAATGG
                     IFVVYLCNFILRFFGIDIPFI"
BASE COUNT          509 a          252 c          352 g          561 t
ORIGIN      
        1 ttctgcaata tatgatgaaa aatcctacca tacgctcttc tggtttgcgg cagcgattgt
       61 ggcagtaact tataaaaaaa gaccttttat gtaacttcct gctattttga ttttttgctt
      121 gagcttacaa acgtatatgg cattctggaa tggaaatgct atctttacta taaaaggtat
      181 tgcccatgaa tatattaata cttggtggtg gtgctgttgg attgggttta gcgcgaagcc
      241 ttattactgc aggcaacgat gtaactatta ttgatgttga accaacggtc agtgcactga
      301 aagaaaaagg tattatgcaa acaggtgtgt tgggggaatt tactatacag cctggtatat
      361 ttaaagtcta tgattcattg gcaaaaatag gcaatgtcaa aattgattat atctgtgtat
      421 gtattaaatc atacttatca gaaactatcg cacaggtatt aaaacaatac gaacatataa
      481 ttggtacctg tcccatagta ttgtttcaga atggatgggg caatgctgaa caatttataa
      541 aatactttga aaaagataca atcttcaatg cgcgtgttat gacaggatac attaagccag
      601 cgcttcaccg tgttgatata acggttcatg ctgatgcaat ccatattggt tcattgtatg
      661 gtggtgatat tacacaatta ctatcgcttg ccagtgagtt aacaaaaggt ggcatgcctg
      721 ctgaagtgac cgaacatgtg agtaaagata tatgggcaaa gatgctgtac aactgcgcgt
      781 taaatccatt aggtgctatc ttcaatgtac cgtatggaga gttaggtaag tctgaatata
      841 caaaagctat catgaatgat atatttcatg agatatttga aaccatgcat aaggcaggtt
      901 acagcacgca ttatagaagt gcagaagaat atattgatgt attttattct cgattgcttc
      961 catcaacggg ggagcatcgt tcatcaatgc tgcaggatat acaggcaaag cgccgtactg
     1021 agatagatgc aatgaacgga gctgttgttg cattagctaa acagcatggt gttgatacac
     1081 ctgttaatga agtgataaca ggattaatta aatttattga gggagggtat catggcaaat
     1141 agtcttgtta gaagcggaaa tcctgtgctc agaagtgatc catttacaaa agaaattggg
     1201 gaacctgcaa ctgatgtaat gaccattgaa ggcgctgtga ataagactat cattttatta
     1261 ctgatagttg ttggttttgc aacgttaagc tggtatcata tagaaaactt gcttttcaat
     1321 aattatctcc atatgtttct gatagttttg tcaattttgg gttttatagt agcacttctt
     1381 actattttta tgaaaaaact atcaccatgg acagcacctg tttattctgc aatacagggc
     1441 ttatttcttg gaatagtatc aatgctattt gaaaaaagtt ttcctggtat agtgttgcag
     1501 gcagttggac ttacctttgg aatacttttt tcgcttcttc tggcttataa atcaggttta
     1561 attaaggcta ctgaaaattt taaacttggt gtggctgctg caacaggtgg aatatttgtg
     1621 gtttatttgt gtaattttat tttacgattt tttggtatag atattccttt tatt
//
LOCUS       DAOSZP010000522         1674 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_53665_length_1674_cov_3.416924, whole genome shotgun sequence.
ACCESSION   DAOSZP010000522 DAOSZP010000000
VERSION     DAOSZP010000522.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1674)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1674)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1674
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53665_length_1674_cov_3.416924"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1072)
                     /locus_tag="PK348_09995"
     CDS             complement(<1..1072)
                     /locus_tag="PK348_09995"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXK66588.1"
                     /translation="MLVTYAIIFLFIIILVIFILYFANIYIFPRKLEEIQQLIESGQT
                     KIAIRKLNDIIEKDDRNHYAHYLLALAYEKENNIQYAILEYRQVLKISKFDDKVDEIV
                     IRKRLASIYKARNAIEEAKKEYLLLTKLDPANYEPFYELGVIFFNAGQLEKAAPYFKK
                     SISNNSRHAPSYYYLGQVQYRMQNYADAKQSFIEAIKIDPNDYKSHYFLGLVLRQLGD
                     YEWAIKEFETAQKSDEIKVKCFLAKGTCFLEREQLPKAVVEFERGLKFAKRGSDTELN
                     LRYFLADAQEKMRDLHSAISNWEKIVAVNPTFRDVQEKLKAYAEFRQDDRIKDFMIAG
                     LAQFEHTCRKMVEAMGLTVMDVD"
     gene            complement(1113..1433)
                     /locus_tag="PK348_10000"
     CDS             complement(1113..1433)
                     /locus_tag="PK348_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015682429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66589.1"
                     /translation="MASEIEKLKKENLSLTEEIAELRKIIQTYENVLKLNEQELSNAY
                     QIIAMYENIVEYSRSELKLATETAQARENASQLSRLELMQAFEKIKQLEEENKRLRAQ
                     KGNA"
BASE COUNT          517 a          327 c          238 g          592 t
ORIGIN      
        1 tatcaacatc cataactgtc aaacccattg cttccaccat tttacggcac gtatgctcaa
       61 attgtgcaag gccagcaatc atgaaatcct taatgcggtc atcctgcctg aactcagcat
      121 acgctttaag tttttcctga acatcacgaa aggttggatt aacagccaca attttttccc
      181 aattcgatat agccgaatga agatcacgca tcttttcctg agcatcagct aaaaaatacc
      241 gcaaatttaa ttctgtatca ctaccccttt ttgcaaattt caatccccgt tcaaattcta
      301 ctacagcttt aggaagctgt tcacgctcta aaaaacaggt cccttttgca agaaaacact
      361 taactttaat ttcatcactc ttttgagctg tctcaaattc tttaatagcc cattcataat
      421 cacctaactg tcgcaacacc aggcctaaaa aataatgtga tttataatca ttgggatcaa
      481 tcttaattgc ttcaatgaat gactgtttag catcagcata gttttgcatt ctatactgaa
      541 cctgccccaa ataataatat gatggagcat gtctactatt attagaaata cttttcttaa
      601 aataaggtgc tgctttctct aattgaccag cattaaaaaa aattacaccc aactcgtaaa
      661 aaggttcata gtttgcagga tctaatttag ttaaaagcag gtactctttt tttgcttctt
      721 caatggcatt acgcgctttg tatatgcttg ccagacgttt tcgaataact atctcatcaa
      781 ccttatcatc aaattttgat attttcaata cctgtctgta ttcaagaata gcatactgta
      841 tattattttc tttttcataa gctaatgcta gaagataatg cgcataatga ttccggtcat
      901 ctttttctat aatatcatta agcttccgta ttgctatttt cgtctggcct gattcaatga
      961 gttgttgaat ttcttctaat ttacgtggaa aaatataaat atttgcaaaa taaagaataa
     1021 aaatgacaag aattattatg aataaaaata ttattgcata tgttaccagc atactctgca
     1081 acctgttgcc ttttttggat tatacattta ccttatgcat ttcctttttg agctctgagc
     1141 cttttgtttt cttcttcaag ctgttttatc ttttcaaatg cttgcattaa ttccaatcta
     1201 ctaagctggg aggcattttc ccgtgcctgt gctgtttcag ttgcaagctt taactcagag
     1261 cgtgaatatt caacaatatt ctcatacata gctataatct ggtatgcgtt cgatagttcc
     1321 tgttcattaa gcttcaagac attttcatat gtctgaatga ttttccgtaa ttcagctatc
     1381 tcttcagtta gactcagatt ttctttcttc agtttttcaa tttcagatgc catagtataa
     1441 cctcaagaaa agtatatcct caaagtaaca tataatatta tactactata atgattatca
     1501 ttttggcaag aataaatcca gtataatact aaaatttatt atatatcata ccaaaaaata
     1561 ttgacaaaat tttatgattg attactacga acataagcgt tcatattcta agcataatcg
     1621 ggggcgtagc tcagttggga gagcgcttga atggcattca agaggtcagg ggtt
//
LOCUS       DAOSZP010000523         1672 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_53759_length_1672_cov_3.185529, whole genome shotgun sequence.
ACCESSION   DAOSZP010000523 DAOSZP010000000
VERSION     DAOSZP010000523.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53759_length_1672_cov_3.185529"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(358..>709)
                     /locus_tag="PK348_10005"
     CDS             complement(358..>709)
                     /locus_tag="PK348_10005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66590.1"
                     /translation="ITDGDANENDFKIYSPDIIIDYAKSHFIPVYILTLKTKSPILMR
                     IAQETGGAIYKGSELDGLRTVYDAIKKANDYRYVLIYSTFKMKSLKGWWSDLTVNVSY
                     KGQKGTEWAGYFVP"
     assembly_gap    710..719
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<720..>1672)
                     /locus_tag="PK348_10010"
     CDS             complement(<720..>1672)
                     /locus_tag="PK348_10010"
                     /inference="COORDINATES: protein motif:HMM:NF028479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="6-bladed beta-propeller"
                     /protein_id="HXK66591.1"
                     /translation="PEGVCFGGDSSLYVVDSGNHRVQKFGLDGRFILAFGQFGEYEGQ
                     LNKPTDVAVRYENVYVTDTNNKRIAVFDDSGNFVENLTPSEFAVPRGIFIQGNLMAVS
                     DEKKGLFMYNFDTGQSQWFVSWEGKKKFYHLTSAVMDDNGFVYTCSNKSEAIYVFSPL
                     QQHISNIEVEVTNVDAKKFPTVAVYCNIRDRYGRPIYGLTKENFTIIEDGATITNLSA
                     DYLKNMMPAASMVMCVDRSGSLKNYHNDVPWLAEFILQKMHKNDQLKIINFADDTWVA
                     NPYDWSRRRALKALKTFDYGKGRDIGKALYTAISDVLPQMS"
BASE COUNT          522 a          339 c          234 g          567 t
ORIGIN      
        1 tgctgaaaaa gggttggatc aaaaacggtt tttaataatg gatattggag aaattaaaat
       61 tttaaaaaaa taaaacctgt taaccaattc tctcttttta aagataaaat ttactgaggt
      121 cccaacaaat ttctttccat taaccaattt tattttgata aaaagcctct ggaatatttc
      181 atccagaagc tttttattaa tacattgatt attatttttt acatagcctt taataacgat
      241 tttttcaatt ttatagtgtt cccaaatttt tcataaaatt cttttgacac tgaatcaaac
      301 tcatcaatta attcaacttt cgttattctg aaaaattcat cacgagtata ttatatttca
      361 tggaacaaaa taaccagccc attcggttcc tttttgccct ttatatgaaa cattaacagt
      421 aagatctgac caccatccct tcaaagactt cattttaaac gttgaataaa ttaatacata
      481 gcgataatcg tttgcctttt ttatagcatc atacactgtg cgtaatccat ctaattcact
      541 ccctttatag attgctccgc ctgtttcctg agctatacgc attaaaattg gcgactttgt
      601 ttttaatgtg agaatataca ctggaataaa atgggatttt gcataatcaa taataatatc
      661 gggggaatag atcttgaaat cattttcgtt tgcatcacca tctgtaatan nnnnnnnnna
      721 gacatctgag gcaatacatc actgattgca gtatacagtg ccttaccaat atcacgccct
      781 ttcccatagt caaatgtttt taatgctttc aatgcccttc gcctgctcca atcatacgga
      841 tttgcaaccc aggtatcatc agcaaagttt attattttca gttgatcgtt tttatgcatc
      901 ttctgcaata taaattctgc aagccatggt acatcattat gatagttctt caatgaacct
      961 gaacggtcaa cacacataac cattgaagct gctggcatca tgtttttaag ataatcagca
     1021 cttagatttg tgattgttgc tccatcctca atgatagtga agttttcttt tgttaatcca
     1081 tatattggtc taccataacg gtcacgaata ttgcaataga ctgccacagt gggaaacttt
     1141 tttgcatcaa cattagttac ttcaacttca atattgctta tgtgttgctg gagtggtgaa
     1201 aacacatata tggcttcact tttattgctg catgtataca caaacccatt atcatccata
     1261 actgccgaag taagatgata aaatttcttt ttcccttccc agctaacaaa ccactgtgac
     1321 tgacctgtat caaagttata cataaaaaga cctttttttt catcactcac agccatgagg
     1381 ttgccctgaa taaagattcc tcgaggtaca gcaaactccg atggtgtaag gttttcaaca
     1441 aaattacctg aatcatcaaa aacagctatt cgtttgttgt ttgtatctgt cacatatacg
     1501 ttttcatacc gcacagctac atctgttggt ttattcagtt gaccttcata ttcaccaaat
     1561 tgtccaaatg ccaggatgaa tcgtccatct aaaccaaact tttgtacacg atgattgcca
     1621 ctgtccacca catataagga actatcgccg ccaaaacaaa caccctcagg gc
//
LOCUS       DAOSZP010000524         1671 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_53808_length_1671_cov_3.832921, whole genome shotgun sequence.
ACCESSION   DAOSZP010000524 DAOSZP010000000
VERSION     DAOSZP010000524.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1671
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53808_length_1671_cov_3.832921"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..374
                     /locus_tag="PK348_10015"
     CDS             <1..374
                     /locus_tag="PK348_10015"
                     /inference="COORDINATES: protein motif:HMM:NF013909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HXK66592.1"
                     /translation="TLSDILKKISQLLQSTSMIYYGDSGKPIKKVACLQGAGSRKINA
                     VISHGSVDCIITGDVGYHDAVFANNNGVAVIDIGHYVSEKPIIDFLYQTVYNYLTKEI
                     HCNDIPLMISNKEHNPMKCGM"
     gene            376..1074
                     /locus_tag="PK348_10020"
     CDS             376..1074
                     /locus_tag="PK348_10020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66593.1"
                     /translation="MSPEIYTMLELQSIWDKVLAAKNEITRSRHAIDFWRNEMKAMEA
                     KMADLRSRVNQLKTNIKQKELELHDVEARIQFLDSRKNSLKSQREFDSMITELETLNQ
                     KKDEIENSMLQYMEDVDAIENELSVLQQEMPKRLNQIENDVEELQNKLELLQKEAEDN
                     ASQFNKQMTELPPDLRARFAKVIETKGNGIVPLENGICMGCHFQVPVYVKDEVAKSKA
                     VTCTNCGRFLYFKN"
     gene            1138..>1671
                     /locus_tag="PK348_10025"
     CDS             1138..>1671
                     /locus_tag="PK348_10025"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF024848.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HXK66594.1"
                     /translation="MLLSIDRVLQLLAEGKSLEKIAELSDVDVSEIQSVIEEARKIIL
                     EYDKQRAKSKIIVKKKSSITENKNQAEVSDNIFEGLEVLSTIPTESVLTMYIDGASKG
                     NPGPAGIGIVIYDGDDRLVGKVSAYIGKKTNNQAEYTALLRAIQLALYFKTRTLKIRS
                     DSELVVRQLSGEYQVSNP"
BASE COUNT          638 a          237 c          330 g          466 t
ORIGIN      
        1 taacattatc cgatatattg aaaaaaatat cacaactatt acagagcact tcaatgatat
       61 actatggaga ttctggtaaa cccattaaaa aagtggcgtg tttgcagggt gcaggttcac
      121 gaaaaattaa tgctgttatt tcacatggtt cagtagattg tattattact ggtgatgttg
      181 gatatcatga tgctgtattt gccaataata atggtgttgc agtcattgat ataggtcatt
      241 atgtatccga gaaaccaatc attgattttt tataccaaac tgtttacaac tacttgacaa
      301 aagaaataca ctgtaacgat atacctttga tgatatcaaa taaagaacat aatccaatga
      361 agtgtggtat gtgaaatgag ccctgaaata tatacaatgc ttgaattaca atctatatgg
      421 gataaggtac tggcagcaaa aaatgaaatt acccgatcac gtcatgccat tgatttctgg
      481 cgaaatgaaa tgaaagctat ggaagccaaa atggcagatt tacgatcaag ggttaatcag
      541 ttaaaaacta acattaaaca aaaagaactt gaacttcatg atgtggaagc acgtattcaa
      601 ttccttgaca gcaggaagaa ttctttaaaa tcacaacgtg aatttgattc aatgattaca
      661 gaactggaaa ctcttaatca aaagaaagac gaaattgaaa atagtatgct gcagtatatg
      721 gaagatgttg atgcaataga aaacgaactg agtgtgttac agcaggaaat gccaaaacga
      781 ttaaaccaga ttgaaaacga cgttgaagaa ttacagaata aacttgaatt actgcaaaaa
      841 gaagcagagg ataatgcctc gcaatttaat aaacaaatga cagagttgcc acctgattta
      901 agggccagat ttgcaaaagt tattgaaaca aaaggcaatg gtatagttcc acttgaaaac
      961 gggatttgta tgggatgcca tttccaggta cctgtgtatg tcaaagatga agttgctaaa
     1021 tcaaaggcag ttacatgtac taattgtggc cgttttttgt attttaaaaa ttaatataac
     1081 atgtgaaaaa aatcttttgt agttgtaggt taaactaccc aggctgagga gaaagtaatg
     1141 ttattgagca tagatagagt tttacagcta cttgccgaag gcaagagtct ggaaaagatt
     1201 gctgaattat cagatgttga tgtaagtgag atacaatcag taattgaaga agcacgtaaa
     1261 attatccttg aatatgataa acaacgtgca aaaagtaaaa taatagtaaa aaagaaaagc
     1321 agtattacag aaaataaaaa tcaagctgaa gtatcagaca atatatttga agggcttgaa
     1381 gttttatcaa caatacctac agaatccgtt cttacaatgt atattgatgg tgcttcaaaa
     1441 ggaaatccag gtcctgcagg tattggcatt gttatatatg atggcgatga caggcttgta
     1501 ggcaaagttt cagcttatat tggtaaaaag acaaataatc aggcagaata tacagcatta
     1561 ttacgggcaa ttcaactggc actgtacttt aaaaccagaa cattaaaaat aagaagtgat
     1621 tcagaactag tagtgcgcca gctgtcagga gaatatcagg taagtaatcc t
//
LOCUS       DAOSZP010000525         1666 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_54033_length_1666_cov_3.198014, whole genome shotgun sequence.
ACCESSION   DAOSZP010000525 DAOSZP010000000
VERSION     DAOSZP010000525.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1666
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54033_length_1666_cov_3.198014"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..676
                     /locus_tag="PK348_10030"
     CDS             <1..676
                     /locus_tag="PK348_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014454223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="activase"
                     /protein_id="HXK66595.1"
                     /translation="GEIYVRRDDFAVDELVKHFSKKGIIAKVSGVSEWIYYCDFIREY
                     EYKKAIKLAPWYKKLFIPEVWSLLRFFIEQWYKHRVEKKVKQALQRTQLIPDTPHDMH
                     HIMDMSQKHFVDLELQSEITVSSGVAATAMQDGYSGIVNISPFACLIGRVIEGILTPW
                     ARIQGYPVMSVEIDGNALPPTTLNKLEIFMLNVMRFKEKPEMKEVVEHPAQTAVAIDR
                     KIIKDL"
     gene            complement(681..1055)
                     /locus_tag="PK348_10035"
     CDS             complement(681..1055)
                     /locus_tag="PK348_10035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66596.1"
                     /translation="MSTLNDLFSSEFLKDNAILIGKIKKPAITKEEANQILEFVKQDI
                     QNHPDHQHFILDVLSVQEVSNPIIGVLMKALQFWKKTRGYALLVMTDDLLQKIMIEQP
                     AMFDFFAVFHTVDEAIAFTKKA"
     gene            1189..>1666
                     /locus_tag="PK348_10040"
     CDS             1189..>1666
                     /locus_tag="PK348_10040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66597.1"
                     /translation="MKTFIHYCIKILSYSVIALCSLVVFVMLILFIANLVLTTDRVSK
                     IITDEVESNLNAKVSCHVTHFSLFNGFEIKNFILTGSDSSKPVVTFDTFRLRYSFFPL
                     LIGKIKIYEVGLYNPSVYLEEQKGIWNIQKLSKPSEKKEKEDKEEAKEEKEKKDEIT"
BASE COUNT          554 a          262 c          326 g          524 t
ORIGIN      
        1 tggcgagatt tatgtccgca gggatgattt tgctgttgat gaacttgtta agcacttcag
       61 taaaaaggga atcatagcaa aagtttcagg agtcagcgaa tggatctatt actgtgattt
      121 catacgtgag tatgaatata aaaaggccat taaactggca ccgtggtata aaaagctgtt
      181 tatccctgag gtatggtcgc tgttgagatt ttttattgag cagtggtaca aacaccgtgt
      241 ggagaaaaaa gttaaacagg cgttacaacg tacacagctt atcccggata ccccgcatga
      301 tatgcaccac atcatggata tgtcacaaaa acattttgtg gatctggaac tgcaatctga
      361 gattactgtt tcaagtggtg tagcagcaac agcaatgcag gatggctatt caggcattgt
      421 caatatatca ccgtttgcct gtttaattgg tcgagttatt gaaggtattc ttacgccgtg
      481 ggctcgaata cagggctatc ctgttatgtc agttgaaatt gatggcaatg ccctgccgcc
      541 aaccacgctg aataaacttg aaatattcat gttaaacgtt atgcgattta aggaaaagcc
      601 agaaatgaag gaagtcgttg aacatcctgc acaaacggca gttgcaattg acaggaaaat
      661 aattaaggat ttataataca ctaagctttt ttggtaaaag ctattgcctc atcaacagtg
      721 tgaaatactg caaaaaaatc aaacattgca ggttgttcaa tcattatttt ttgcagcaga
      781 tcatccgtca ttaccagtaa tgcataacca cgtgtctttt tccagaattg cagtgctttc
      841 attaacacac caataatagg attggaaacc tcctgcaccg ataacacatc cagaataaaa
      901 tgctgatggt caggatggtt ttgtatatct tgcttgacaa attctaagat ttgattagcc
      961 tcttctttgg tgattgcagg ctttttgatc ttcccaatga gaatagcatt atcttttaaa
     1021 aattcagaag aaaaaagatc atttaatgta ctcatagttt acccttatgt ataaatattt
     1081 atgtcatgat acaaagccaa taaatgatat gtcaatgaaa aaaagtttta ctatattggt
     1141 taacagtgta gtgattatgc tttatactgg agaatgtgga gcaatcgttt gaaaacattc
     1201 atacattatt gtattaagat tctttcatat agtgttatag cactatgtag ccttgttgtt
     1261 tttgtaatgc tgattctttt tattgccaat cttgtgttaa ccactgatag ggtatcaaaa
     1321 attattacag atgaagttga gtccaatctc aatgcaaaag tttcatgtca tgtaacacat
     1381 ttttcattgt tcaatggctt tgaaataaaa aattttatac ttacaggtag tgatagttca
     1441 aaacctgtag taacctttga tacatttcgg ttgcgttata gttttttccc attgttaata
     1501 ggtaaaatca aaatatatga agttggattg tataacccct ctgtatatct tgaagagcag
     1561 aaagggatat ggaacattca aaaattgagt aaaccttctg aaaaaaaaga aaaggaagat
     1621 aaggaagaag ctaaggagga aaaggaaaaa aaggatgaga ttacat
//
LOCUS       DAOSZP010000526         1665 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_54088_length_1665_cov_2.057764, whole genome shotgun sequence.
ACCESSION   DAOSZP010000526 DAOSZP010000000
VERSION     DAOSZP010000526.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1665
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54088_length_1665_cov_2.057764"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..424
                     /locus_tag="PK348_10045"
     CDS             <1..424
                     /locus_tag="PK348_10045"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HXK66598.1"
                     /translation="KGWNDHWMITFGATYKWGGWSPIDSSHAKAKQLESQANQLDLQL
                     QDFVKSVRLDIQRGFLKLKSASNSLYSQQGNVETAEESLKTATVQFRNGIIDNSKFLE
                     ANVSLIQAKTLYIQALYDYKVAQAELNKAIGVDYFKIQ"
     gene            458..1381
                     /locus_tag="PK348_10050"
     CDS             458..1381
                     /locus_tag="PK348_10050"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HXK66599.1"
                     /translation="MKKGTKIIIVLILLIIAIIGTYRLVVFIGNKIKPKQTAQEERIL
                     PVKAAKAVRMDITKTLNLSGDIVGKEVVNVFSQVPGKVMEILVKEGQFVKKGQVLFRV
                     DRDIVGMEYMPAVVESPLSGYVGTVMVDRGMSISPQTPLAQVVTMNQVEAVAQLIEEQ
                     INMVQIGMKAKVRVDAYPNVYFYGTVYRKNAVLDAASRTQEVRILIDNPELKLRHGMF
                     ADIQIQMGSAKNAVVIPVDSILSDSSGNYYVYKVVDNKAFKQAIKPGFSLKNVIEVVS
                     GINEGDVVVTLGKENVGHGDKLMVYMEEIKQ"
     gene            1403..>1665
                     /locus_tag="PK348_10055"
     CDS             1403..>1665
                     /locus_tag="PK348_10055"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HXK66600.1"
                     /translation="MKLTDISVQRPITITMLYIGIIVVAIVSYVKIPLDLFPDIEFPV
                     AVVFTEYPNVGPKEIESTVTRPLEEALSSVNNIDTITSTSKEGL"
BASE COUNT          538 a          267 c          397 g          463 t
ORIGIN      
        1 taaaggctgg aatgatcact ggatgataac ctttggtgct acctataagt ggggtggatg
       61 gtcaccaatt gattcatcac atgcaaaggc aaaacaactg gaaagccagg caaaccagct
      121 tgatttgcag ttacaggatt ttgtgaaaag tgtgcgactt gatattcagc gcggattttt
      181 aaagctgaaa tcggcttcca attctttgta ttcacagcag ggaaatgttg aaactgcaga
      241 ggagtcattg aaaacggcaa cagtgcagtt tagaaatgga attattgata actcaaaatt
      301 tttagaggca aatgtatcgc ttattcaggc taagaccttg tatatacagg cgctgtatga
      361 ttataaggtt gcacaggctg agttgaacaa agcaattggt gtggattatt ttaagattca
      421 ataaaagata gttcaacgat tttatggagg ataaataatg aagaaaggaa ccaaaatcat
      481 aatagtctta attcttctca ttatagcaat cataggaacg tacagattgg tagtatttat
      541 tggtaataaa attaagccta aacagactgc ccaggaggaa cgtattttac ccgtaaaagc
      601 agcaaaggca gtgcgcatgg atataacaaa aacccttaat ttaagcggtg atattgtggg
      661 caaagaggtt gtgaacgtct tttcacaggt gccgggcaag gttatggaaa tccttgtaaa
      721 ggaaggccag tttgtaaaaa aagggcaggt acttttcagg gttgatcggg atatcgttgg
      781 tatggaatac atgcccgcag ttgttgaatc gccactctca ggatatgtag gcaccgtgat
      841 ggttgatagg ggaatgtcca taagcccgca gactccactt gctcaggttg ttaccatgaa
      901 tcaggttgag gcggttgctc agctcattga ggagcagatc aacatggtac agattggcat
      961 gaaggcaaag gtacgggttg atgcatatcc caatgtatat ttctatggca cagtgtatcg
     1021 taaaaatgcg gtacttgatg cagcaagtcg aacgcaggaa gttcgtatac tcatagataa
     1081 tccagagctt aagctacgcc atggcatgtt tgcagacatt cagatacaga tgggttcagc
     1141 aaagaacgcg gtggtgatcc cggtagatag tattttaagc gatagctccg gtaattatta
     1201 tgtatacaag gtagttgata acaaggcatt taaacaagca ataaagcccg gattttcctt
     1261 aaaaaatgtt atcgaagtag tatccggtat taatgaaggt gatgtggttg ttacactggg
     1321 caaggaaaat gtggggcatg gcgataagct tatggtttat atggaagaaa ttaaacagta
     1381 atagtgcagg gagatagtta ctatgaaatt aactgatata tccgttcaaa ggccaataac
     1441 aatcacaatg ttatacattg gtataattgt agtggcaata gtgagttatg taaaaatccc
     1501 tttagattta tttcctgata ttgaattccc cgttgctgtt gtttttaccg agtatcccaa
     1561 tgttggtcct aaagagatag aaagtaccgt aacccgacca ctggaagagg cattgtcatc
     1621 ggttaataat attgatacca taacgtcaac atcaaaagaa ggcct
//
LOCUS       DAOSZP010000527         1660 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_54289_length_1660_cov_2.783178, whole genome shotgun sequence.
ACCESSION   DAOSZP010000527 DAOSZP010000000
VERSION     DAOSZP010000527.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1660
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54289_length_1660_cov_2.783178"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(79..618)
                     /locus_tag="PK348_10060"
     CDS             complement(79..618)
                     /locus_tag="PK348_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP oxidoreductase"
                     /protein_id="HXK66601.1"
                     /translation="MSKPVVATASLAGCFGCHMSILDIDDRILKLIELVEFNKSPIDD
                     IKTFTKQCDIGLIEGGCCNSENVENLKLFRKNCKILISVGECAIMGGLPAMRNGIPVK
                     ECLEEAYLKCPTVDDNIIPNDDELPMILDRVYPCHEIVKIDYFLPGCPPRADLIWEAL
                     VALLTGKELELPYEVVKFD"
     gene            complement(647..1342)
                     /locus_tag="PK348_10065"
     CDS             complement(647..1342)
                     /locus_tag="PK348_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011394105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HXK66602.1"
                     /translation="MSTINFTIDGKACKAKPGQTIVEAAKDNGIYIPTLCHFEGLKPA
                     GTCRICTVKVGGRNMAACTTPVTEGMVVENKTADLEDYRKAIIEMLFVEGNHMCPTCE
                     KSGNCELQALGYRYMMMAPRFPFLFPRRTLDASLPKIVIERNRCVQCLRCVRGITTAD
                     GKRIFGVVNRGGHVEITVDKDLAATMTDEMAKKAMDLCPVGAILKKEVGFAIPIGKRK
                     FDSKPIGSEIEKA"
     gene            complement(1344..>1660)
                     /locus_tag="PK348_10070"
     CDS             complement(1344..>1660)
                     /locus_tag="PK348_10070"
                     /inference="COORDINATES: protein motif:HMM:NF022059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HXK66603.1"
                     /translation="CGWCVPCRAGNVILKQKLEKIMSGKASTKDVQELEDWCKIVKAT
                     SRCGLGQTSPNPIYTTIQNFREMYEAKVKKDTDYVSTFDLLAAVEESCKAAGRIPKVE
                     EH"
BASE COUNT          458 a          400 c          260 g          542 t
ORIGIN      
        1 tttaccatga ccttctaccc ttgttacagg ttctatcgtt atttttctag ccataagctt
       61 tcttctccta aaacaaaatt agtcaaactt tacaacttcg tacggtaatt ccaactcttt
      121 accggtaaga agtgctacca gtgcttccca gattaaatct gcacggggag gacatcccgg
      181 caagaaataa tctattttaa ctatctcatg acaaggatat accctgtcca gaatcattgg
      241 taattcatca tcattaggaa tgatattatc atctacagtt gggcatttca agtatgcttc
      301 ttccaggcat tcttttacgg gaataccatt gcgcatagct ggtaatccac ccataattgc
      361 acattcacca actgatataa gtattttaca atttttgcgg aataacttaa ggttttcaac
      421 attttcactg ttacagcatc caccttctat taatccaata tcacactgtt tggtgaacgt
      481 tttaatatca tcaattggtg acttgttaaa ttcaaccaat tcaattaact ttaatattct
      541 atcatcaatg tccagaatgg acatatggca tccaaaacat cctgccagtg aagcagttgc
      601 gacaactggt ttactcattg tattcctcca tagatcttca ttaaaatcag gctttttcaa
      661 tttcactgcc aataggcttg ctatcaaact tacgcttgcc aattgggata gcaaaaccaa
      721 cttctttctt caatatagca ccaaccgggc acaaatccat tgccttcttg gccatttcat
      781 cggtcatggt tgctgctaaa tctttatcaa ccgtaatttc aacatggcca cccctattga
      841 caacgccaaa aatacgcttg ccatcagctg tcgttatacc ccgcacacaa cgtaaacact
      901 gcacacacct gtttcgttca atcacaatct ttggtaatga tgcatcaagc gttcgtctgg
      961 ggaaaagaaa cggaaaccgt ggcgccatca tcatataacg gtatcccagc gcctgtaact
     1021 cgcaattacc gcttttttcg catgttgggc acatatgatt gccttcaaca aatagcatct
     1081 caattatagc tttgcggtaa tcttccagat cagcagtttt gttttctacc accatacctt
     1141 cagttacagg tgttgtacag gcagccatat tcctgcctcc aactttaaca gtacagatac
     1201 ggcaggtacc agctggtttt aacccttcaa aatggcataa tgttggaata tatatgccat
     1261 tatcctttgc tgcttcaaca attgtctggc cgggtttagc cttacatgct tttccgtcta
     1321 tagtaaaatt tatagtactc atattagtgt tcctctacct ttgggattct tcctgctgct
     1381 ttgcatgatt cttcaacagc agctaaaaga tcgaatgtgg atacataatc agtatccttc
     1441 ttaacttttg cctcatacat ttctctaaaa ttctgaatag ttgtatatat tggatttggt
     1501 gatgtctgtc ccaaaccgca gcgactggtg gctttcacta tcttacacca atcttcaagc
     1561 tcctgaacat cttttgttga tgctttcccc gacattatct tttctaattt ctgcttcagg
     1621 ataacattac ctgctctaca gggtacgcac catccacagg
//
LOCUS       DAOSZP010000528         1659 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_54325_length_1659_cov_3.538030, whole genome shotgun sequence.
ACCESSION   DAOSZP010000528 DAOSZP010000000
VERSION     DAOSZP010000528.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1659
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54325_length_1659_cov_3.538030"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..571
                     /gene="hprK"
                     /locus_tag="PK348_10075"
     CDS             <1..571
                     /gene="hprK"
                     /locus_tag="PK348_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002155384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HXK66604.1"
                     /translation="LLTHIVEAAFAESVTIHGTLIDVFGIGILLIGKSGVGKSETALE
                     LIERGHRLIADDLVEIKKIDESLLMGSGSELIRHHMETRGIGILNVRDIFGIRSVRNR
                     KRIELVAELQEWDANTHYDRLGIEEQVYTILDIEVPYIIVPVRPGRNIPIIIETAALN
                     QRLKKMGVFSAQELDKKIQEWIARENNNK"
     gene            568..843
                     /locus_tag="PK348_10080"
     CDS             568..843
                     /locus_tag="PK348_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008193712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HXK66605.1"
                     /translation="MKEKKVKVKSDAGVHARPASILVREAMKFPCDIYIIKGQMEANG
                     KSIMSVLGLAITKGSSLIIRANGEMEEEAVDRLSTLIENDFAELASK"
     gene            840..1421
                     /locus_tag="PK348_10085"
     CDS             840..1421
                     /locus_tag="PK348_10085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66606.1"
                     /translation="MKTHGIYCIIWLLIAFILVLQSPLQAQTKKKEAPSQELSLTDDE
                     IKVLFEKKKDPLFAGILSFYMPGLGQFYSDEKLKGALFLVTEYTIVIGSLFYFLDFSF
                     KAGSDSGFNLGIDAKRTDLGVISTQRKYLFYATVATLSLIHIYNIIDAVQSAGSFNTS
                     LQEKRQELIKKYPQLNFGYNGDGGMYFGLSSRF"
     gene            1399..>1659
                     /locus_tag="PK348_10090"
     CDS             1399..>1659
                     /locus_tag="PK348_10090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66607.1"
                     /translation="MGYRLVSKISLVAIIILSLFSIAFAQKDKQIQQNKESAVQSNGT
                     PIIFEETERKDPFIAGVLSWSWRGLGQFYTQNYKKGSAFLLAD"
BASE COUNT          530 a          258 c          376 g          495 t
ORIGIN      
        1 cttgttaacc catatcgttg aagctgcatt tgcagaatca gtaaccatac atggtacatt
       61 gattgatgta tttggtatag gaatattgct tattggtaaa agtggtgttg ggaaaagtga
      121 aactgcttta gagttaatag aaagaggaca ccggctgatt gcagatgacc tggttgagat
      181 taaaaaaatt gatgagtcat tactgatggg aagtggttct gagcttatac ggcatcatat
      241 ggagacacgg ggaattggaa tattaaatgt tcgtgatata tttggtatca ggtcagtaag
      301 gaacagaaag cgtattgagc ttgttgcgga attacaggag tgggatgcaa atacccacta
      361 cgacaggctt ggtatagaag agcaggtgta taccatcctt gatattgaag ttccgtatat
      421 tattgttcca gtaaggcccg ggcggaatat cccgataatt atagaaactg cagcacttaa
      481 tcaacggtta aaaaaaatgg gcgtgttttc cgcgcaggaa cttgataaaa agattcagga
      541 gtggattgca agggagaata acaacaaatg aaggaaaaga aagttaaggt aaaaagtgat
      601 gctggtgttc atgcacgtcc tgcatctata ctggtacggg aggcgatgaa attcccatgt
      661 gatatctata tcattaaagg ccagatggaa gcaaacggca aatccataat gtcagtgctg
      721 ggtctggcta taaccaaggg aagttctctg ataataaggg ctaatggcga aatggaagaa
      781 gaagctgtag accgattatc aactctcatt gaaaatgatt ttgcggagtt agcttcaaaa
      841 tgaaaactca tggtatatat tgtataatat ggctgttgat tgcattcata ctggtattac
      901 aaagccctct tcaagcgcaa acaaagaaaa aagaagctcc ttctcaggaa ctatcgctaa
      961 ctgatgatga aatcaaggtg ctttttgaaa aaaagaagga cccccttttt gccggtattc
     1021 tttcttttta tatgccaggc cttggccaat tttatagtga cgaaaaatta aaaggtgctc
     1081 ttttcctggt tactgaatat accatagtga taggatcact tttttatttt cttgatttta
     1141 gttttaaagc tggaagtgat tcaggtttta atttaggaat tgatgcaaag cgtactgatt
     1201 taggtgtcat cagcactcag cgaaaatatc tgttttatgc tacagttgcc acactatcac
     1261 taattcatat ttataacata attgatgcag ttcaaagcgc cggttccttt aacacttcgt
     1321 tacaggaaaa acgacaggaa ttgataaaaa agtatccaca acttaacttt ggatataatg
     1381 gtgatggagg tatgtatttt gggttatcgt ctcgtttcta agataagttt ggttgcaatt
     1441 atcatactat ctttgttttc aattgcattt gcccagaagg ataaacaaat acagcaaaat
     1501 aaagaatctg ctgtgcaaag taatggtact cctataattt ttgaagaaac tgagcgaaag
     1561 gacccattca tagcgggtgt gctgtcgtgg tcatggagag ggttgggaca gttctatact
     1621 cagaattata aaaagggctc agcctttttg cttgctgac
//
LOCUS       DAOSZP010000529         1654 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_54575_length_1654_cov_3.042527, whole genome shotgun sequence.
ACCESSION   DAOSZP010000529 DAOSZP010000000
VERSION     DAOSZP010000529.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1654
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54575_length_1654_cov_3.042527"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            342..1394
                     /locus_tag="PK348_10095"
     CDS             342..1394
                     /locus_tag="PK348_10095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66608.1"
                     /translation="MKKIIIIVLVFVCTAAYARTTVNLTPADANFSDIPVLEDSLGKQ
                     VQDVTDKNGKFIANSFALANMLGYPIGKSSIGHFPHMEAGIALGVALTNAKYFDDKAE
                     DGTFPGVMANPVVHAGFGLAGNFDIIGKLFYFRKNIYDPDIDTDTARLQDFNFISFGA
                     KLRYNYLKEATILPFLLSFGGLTFSIGGDIMMGNVDVTGEYDAEYEDITVTYSSIDYP
                     LSANLNSTYGATVSWTVFTLSAQAIAYIDVMYLFSFYTGFGLATNLGFFSTDFNGNGQ
                     LTSDDPTFLGVKADGLIGTMEFESVNSYMPGYLIPTFIFGVEINLFVIKLTADTMVNL
                     ANRSDVTLQGGVRMQL"
     gene            complement(1404..>1654)
                     /locus_tag="PK348_10100"
     CDS             complement(1404..>1654)
                     /locus_tag="PK348_10100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="short chain dehydrogenase"
                     /protein_id="HXK66609.1"
                     /translation="DKRQEILAQKFFEKTFATVDDVAKHIIHSISRKKLYIITQPDAK
                     AVWWVKRHFPNFYARILSFAYRRGIVYMYLGVKPEELA"
BASE COUNT          459 a          330 c          341 g          524 t
ORIGIN      
        1 tcgatacgat tgccatgcaa tcactcggcg accgcgcctc gatacgattg ccatgcaatc
       61 actcggcgac cgcgcctcgg tacaattgct tagcaagcgt tcggtaagtg cgccttgatg
      121 taattgctta gcaatcgctg gtgaccgcat ctttatatga tcgccatgca atcactcggc
      181 aatcagcctg tcgaagtctg gaatgacgca tcaaattgat tccgggtcaa gtcgaaaatg
      241 acggtaggga acaatgaaca tacataacaa aaatataaaa gcttcataat aatgtttgac
      301 atctttttat ttttttaaga gtatatattg cggaggatgt tatgaagaag attattatca
      361 ttgtacttgt gtttgtatgc accgctgcct atgccaggac aactgttaat ttaacgcctg
      421 ctgatgcaaa tttttctgac ataccagttc ttgaggattc tttaggaaag caagttcaag
      481 atgtaactga taaaaacggc aagtttattg ccaattcttt tgctctggcc aatatgttag
      541 gatacccaat aggcaaatca tccattggtc attttccgca catggaagct ggtattgccc
      601 ttggtgttgc gctgaccaat gccaaatatt ttgatgataa ggcagaagac ggcaccttcc
      661 cgggtgtaat ggcaaaccct gttgtccatg ctggttttgg actggcaggc aattttgata
      721 tcattggcaa gcttttttat ttcagaaaaa atatctatga tcctgatatt gatactgata
      781 ctgcacgttt acaggatttt aattttatct catttggtgc aaagctacgg tataattatc
      841 ttaaagaagc aaccatactg ccattcctgc tttcatttgg aggtcttacc ttcagtatcg
      901 gcggtgatat catgatgggc aatgtggatg taaccggtga atatgatgct gaatatgagg
      961 atattacagt tacttatagt tcgatagatt accctttatc ggcaaatctc aatagcacct
     1021 atggtgccac ggtaagctgg acggtgttta cacttagcgc tcaggctatt gcatatattg
     1081 atgtcatgta tctattcagc ttttatactg gctttgggct ggctacaaat ctgggatttt
     1141 tcagcactga tttcaatggg aatgggcagc ttacatctga tgatcccact ttccttggag
     1201 taaaagcaga tggactcatt ggtaccatgg aatttgaatc ggtcaatagc tatatgccag
     1261 gctatcttat tcccaccttt atctttggtg ttgaaattaa cctttttgtc ataaaactta
     1321 ccgctgatac catggtaaat ttagctaacc gcagcgatgt taccctgcag ggtggagtac
     1381 gaatgcagct ataatatatt tccttatgct aattcttcag gcttaacacc aagatacata
     1441 taaacaattc cacgcctgta tgcaaacgaa agtattctgg cgtaaaaatt tggaaaatgt
     1501 cgtttaaccc accacaccgc ttttgcatca ggctgggtaa tgatgtaaag ctttttcctg
     1561 gaaattgaat gtataatgtg ctttgcaaca tcatcaaccg ttgcaaatgt tttttcaaaa
     1621 aatttttgag caagtatctc ctgccgcttg tcag
//
LOCUS       DAOSZP010000530         1652 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_54664_length_1652_cov_2.780839, whole genome shotgun sequence.
ACCESSION   DAOSZP010000530 DAOSZP010000000
VERSION     DAOSZP010000530.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1652
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54664_length_1652_cov_2.780839"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(202..843)
                     /locus_tag="PK348_10105"
     CDS             complement(202..843)
                     /locus_tag="PK348_10105"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HXK66610.1"
                     /translation="MFLPYKDENPTQSFPLFTIVLIVLNCVVYYLEVYGQPGFENLIK
                     MYALIPCNVNRINVYNYNHYIPFISHMFLHVNIIHVASNMLFLWIFGNNIEDVLGVIR
                     FYGFYILCGLAAALTFYLFHHNECTPIIGASGAVSGLLGGYVVLFPAARIRVWFMFFI
                     LRLPAWFFILLWFVMQLSNLYGEKLGMTNVAWSAHVGGFIAGFILIIFFKKKY"
     gene            complement(845..>1652)
                     /locus_tag="PK348_10110"
     CDS             complement(845..>1652)
                     /locus_tag="PK348_10110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66611.1"
                     /translation="IIDFAHLIERFAKMKKPAALFKMHFEGKARMAHRILVSTKKALL
                     ATIRQALKHEHTSPNIFEMIINMMVNEGLPKLEFSCMYWPLGTIVDYYNYNYEVINNP
                     EFSKHVFNGHIHSRIQLYRYSMLGPHAKVVQTFLSDSSYINGEVVNSIIFPGVEIAEG
                     AVIRDSIILPYNKIGAKARIMRSIIDERTVQPFDQTPLTIGNGCKIGTTEEGMKNSDY
                     PKVLYNSITLIGKNCIIPDAITIGGCCYVSPGKTQADFEKRKYIYNGTSV"
BASE COUNT          556 a          344 c          277 g          475 t
ORIGIN      
        1 aacaaagcat gcaacgttta taacatctcg ataagaattt tttcattgtg aaatactaat
       61 aattttaaat cttcaatgac aaaagcatag cggcattttt ataaaacaac atatcttcaa
      121 gtgtcgtatt gcctttgcat gccaatttca cagttttaat tgctggtggc ctatatccaa
      181 acggccagtc tgatgcatac attaatattt ctttttaaaa aaaataatta aaataaatcc
      241 cgcaataaaa ccaccaacat gtgccgacca ggctacattg gtcataccta atttttcgcc
      301 atacaggttg gaaagctgca tgacaaacca cagtaatata aaaaaccagg ccggcagacg
      361 taaaataaaa aacataaacc acacccggat gcgagctgca ggaaacaaca ccacataccc
      421 acctaaaagc cccgataccg cacctgaagc cccaatgata ggtgtacatt cgttatggtg
      481 aaaaagataa aacgttaaag cagcagcaag cccgcacaaa atataaaacc cataaaaccg
      541 gataaccccc agaacatctt caatattatt gccaaatatc cataaaaaga gcatattaga
      601 tgccacatgg attatgttta catgtaaaaa catgtggctt ataaaaggaa tataatgatt
      661 gtaattatat acattaatcc tgtttacatt gcagggaatt agtgcataca tctttatcaa
      721 gttttcaaaa cctggctgcc cgtaaacctc aagatagtaa accacacaat tcagaacaat
      781 aagtacaatc gtaaaaaggg gaaatgactg tgtaggattt tcatctttgt acggtaagaa
      841 catgtcacac cgaagtaccg ttgtagatat attttcgttt ttcaaaatca gcctgagttt
      901 tacccggcga tacgtagcag catccaccta tggtaatagc atcaggaata atacaatttt
      961 tcccaattaa tgttatactg ttgtacagca ctttagggta atcgctgttt ttcatacctt
     1021 cttcggttgt gccaatttta caaccattcc cgatggtaag cggtgtctgg tcaaatggct
     1081 gcacagtgcg ctcatctata atgcttcgca tgatgcgcgc ttttgcaccg atcttattat
     1141 atggcaggat aattgaatca cgaatgactg caccttctgc aatttcaaca ccagggaaaa
     1201 ttattgaatt aaccacctcg ccgttaatat aggaactatc ggacaaaaat gtttgcacca
     1261 cttttgcatg aggccccaac attgaatagc ggtataactg tatcctggaa tgtatatggc
     1321 cattgaaaac atgtttagaa aattcaggat tattgataac ctcataattg taattataat
     1381 aatcaactat cgtgcccagt ggccagtaca tacatgaaaa ttcaagtttt ggcaacccct
     1441 catttaccat catattaata atcatttcaa agatgttagg tgaagtatgt tcatgcttca
     1501 atgcctgcct gattgttgca agcaacgctt tttttgtgct taccagtata cggtgcgcca
     1561 tacgtgcctt cccttcaaaa tgcatcttga agagcgcagc aggttttttc atttttgcaa
     1621 accgttcaat caggtgagca aaatctataa tg
//
LOCUS       DAOSZP010000531         1650 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_54743_length_1650_cov_7.495925, whole genome shotgun sequence.
ACCESSION   DAOSZP010000531 DAOSZP010000000
VERSION     DAOSZP010000531.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1650
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54743_length_1650_cov_7.495925"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(512..>1650)
                     /locus_tag="PK348_10115"
     CDS             complement(512..>1650)
                     /locus_tag="PK348_10115"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HXK66612.1"
                     /translation="SYRDKDNDIVHNPIREFIPDINILPFPARHLVELSKYRALGFPI
                     NMMTSRGCPFNCIFCVGSKMVGKKVRYFETHRVVDEFEQLAAMKFKQINVVDDLFTSN
                     KSRCIEVCNEIKRRGIDHPWTAFARVDTVNEELLTCMRDAGCTMLCFGIESGNQEILD
                     KVKKKITLEKCKKAVETCRKVGISPMTSYILGLPGETEETMKKTIQFAKELSDNYGFH
                     VLAPFPGTEVREMKDYYGIEILTDDWDLYDANRAVAVTPDVSANKINEIVDVFNGSIR
                     EYILSLGEKQNRGESLTQNEQNLIGNIRSFEIARDIILERLIENHPGIANGAPIDVAH
                     ELTEYLSATLPFPKDELSREVERLIQLDCLQFKYQDNRLYPQWT"
BASE COUNT          518 a          357 c          263 g          512 t
ORIGIN      
        1 cgccatggaa ttttcataca ccatggacat ggcattatat aatcataccc cgtcgctatc
       61 ataagaggtt acgagccatt tcagatgcaa tcgagaaaaa ggcaaataca acaatgccat
      121 ggttaaagca ttttgcggta accctgatga taaaggcagt taaataaata gcttaaaaaa
      181 agggattaat caaccatacc gaggtaattg ctgtgtcata aggatttttt tcagtatttt
      241 cattggcatt ccccggtaaa catagaatta tttgtcattg tcattatgtg atttttttga
      301 ggagtattac aatgtgccct cactgtcacc tatagcgatg ttaccaattg taatcattca
      361 ttgcaacatt attaaaaaaa agaataaaac aagttcccta tgcttatgac caccacaagg
      421 gaacatctat actacaagta gtagatgttc cccttttttg cagaatatac atacaataat
      481 gttatctatt tcaatggttc tttaacaaac tttatgtcca ctggggatac aatctgttgt
      541 cctgatattt aaactgtaaa caatcaagtt gtataagacg ttccacttcc cgtgataatt
      601 catctttggg aaaaggcaat gttgctgaaa gatattcggt caattcatgg gcaacatcga
      661 ttggtgcacc atttgcaatc ccagggtgat tttcaatcaa tcgttctaaa atgatatcgc
      721 gagctatctc aaaagaacgt atattgccaa ttaaattctg ttcattctgt gttaatgact
      781 caccccgatt ttgcttttca cccaatgaca atatgtattc cctgatacta ccattaaata
      841 catcaacaat ttcattaatt ttgttagcgc ttacatcagg ggtaactgcg actgctctgt
      901 ttgcatcata taagtcccaa tcatccgtta aaatttcaat cccataataa tccttcatct
      961 cacgaacctc agtgcctgga aatggagcca gaacatggaa accataatta tcgctcaatt
     1021 ccttagcaaa ctgtatcgtc tttttcatgg tttcttctgt ttcaccaggc aaacccagga
     1081 tgtatgatgt catgggacta attcccactt ttcggcaagt ctcaaccgct tttttacact
     1141 tttccagtgt aatctttttc ttaactttat ccagaatttc ctgattccca ctttcaatgc
     1201 caaaacaaag catggtacag ccagcatccc tcatgcaggt taacagctct tcattaacag
     1261 tatcaacccg ggcaaatgca gtccatgggt gatcaatccc gcgcctttta atctcgttgc
     1321 aaacctcaat acaacgactt ttattggatg taaaaagatc atccacaaca ttaatctgct
     1381 taaatttcat tgctgctaac tgctcaaatt catccacaac acgatgtgtt tcaaaataac
     1441 gaaccttttt cccaaccatc ttgctgccaa cacaaaaaat acagttaaat gggcaaccac
     1501 gggaggtcat catatttatt ggaaacccta acgcgcgata tttggatagc tcaaccagat
     1561 gccgtgctgg gaaagggagt atattgatat caggaataaa ttcccgtatt ggattatgaa
     1621 caatatcatt atccttatcc cgataagaaa
//
LOCUS       DAOSZP010000532         1649 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_54783_length_1649_cov_5.065872, whole genome shotgun sequence.
ACCESSION   DAOSZP010000532 DAOSZP010000000
VERSION     DAOSZP010000532.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1649
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54783_length_1649_cov_5.065872"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="PK348_10120"
     CDS             complement(<1..245)
                     /locus_tag="PK348_10120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HXK66613.1"
                     /translation="MKKALMAFCDSDGAIYKHPAAVAAFRSGRRFVAVEQEELLPLPD
                     GSTLYVLPGRFPVEGRSFKPVSNFNGNEILAVAAFLSS"
     gene            complement(317..829)
                     /locus_tag="PK348_10125"
     CDS             complement(317..829)
                     /locus_tag="PK348_10125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66614.1"
                     /translation="MKDKFHALTESLLTQEEWQHCIFLLFCLGDSSCVEPLLEELQTR
                     GYIVKENDNRVITQKGKQYLSGLMQKLAQFHNAGEIIFQHYVEEELSPIKKEIHAIRM
                     QWRTVRDKRLARKAELQKMGKDKAAVHKDSIYKALQKEQDALRTHLKHAEKKYHKKLA
                     NHVKNIGMLP"
     gene            complement(826..1023)
                     /gene="thiS"
                     /locus_tag="PK348_10130"
     CDS             complement(826..1023)
                     /gene="thiS"
                     /locus_tag="PK348_10130"
                     /inference="COORDINATES: protein motif:HMM:TIGR01683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HXK66615.1"
                     /translation="MKIRVNGIDIEFEKKTIKELLDLYRFNPSKIMVEKNGRIIDKEH
                     YATEVLKDGDVLEIARFVGGG"
     gene            complement(1036..>1649)
                     /locus_tag="PK348_10135"
     CDS             complement(1036..>1649)
                     /locus_tag="PK348_10135"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HXK66616.1"
                     /translation="QIIDNNGMQYHATITQIEDGKIVATITEKLNYPDINVHCTLCAA
                     LLKGKKFDLVIQKAVEIGVKQIIPVVTQHTIPDVSGKEDKKVERWQLIASEASKQCLR
                     KEVPPVHSVMPFEDVIKLPKEIGIMAHADPKSINLRKFLKEQIKHNNVMLLIGPEGGF
                     SKKEVEIARQYDWFVLYNGATQLRAETAAIVLPAIILYEWGYE"
BASE COUNT          442 a          376 c          268 g          563 t
ORIGIN      
        1 gagcttaaaa aagcagccac agccaaaatt tcattgccat tgaaattgct cacaggctta
       61 aacgatcttc cttcaacggg gaaccgcccc ggcaatacat acaatgtact cccatcggga
      121 agtggtaata gttcttcctg ttcaacggca acaaaccgcc tgccacttct aaacgctgca
      181 acggcagcgg gatgcttata gatagcgcca tcactatcac aaaatgccat caacgctttt
      241 ttcatacaat cattttacac cacatccaat aatatcattg catcaccatt cagtatacac
      301 tcccgtatga tgtatttcag gggagcatcc ctatattttt tacatgattt gctaattttt
      361 tatgatactt tttttcggca tgtttaaggt gtgtgcgcaa tgcatcctgt tctttttgca
      421 gcgccttata aatactatcc ttatgcacgg ctgctttgtc tttccccatt ttttgcaatt
      481 ccgctttacg cgctaatcgt ttatcacgaa cagtgcgcca ttgcatgcgt attgcatgta
      541 tttctttttt tattggcgat agttcctcct caacatagtg ctggaaaata atttcacctg
      601 cattatgaaa ttgcgccagc ttttgcataa gccctgatag atattgtttg cccttttgag
      661 taatgacacg attatcattt tctttgacga tatagccccg tgtttgtaat tcttcaagca
      721 atggttcaac acatgagcta tcgcccaaac aaaacaataa aaaaatgcaa tgttgccact
      781 cttcttgtgt aagcaaagat tccgtcagag catggaattt gtctttcatc ccccaccaac
      841 aaatcgtgct atttcaagaa cgtcgccatc tttcaaaact tcagttgcat aatgttcttt
      901 atcaatgatt cgcccgtttt tctcaaccat gattttagat ggattaaacc tgtaaagatc
      961 aagaagctcc ttaatggttt tcttttcaaa ttcaatatct atcccgttta ccctgatctt
     1021 catgctacta ccctgttatt catatcccca ttcatacaaa atgattgctg gcaaaacgat
     1081 agctgcagtt tctgccctga gctgtgttgc accattatat agtacaaacc aatcatactg
     1141 tcgtgcaatt tcaacctctt tttttgaaaa acccccttca ggtcctatga gcagcataac
     1201 gttgttatgt tttatctgtt cttttaaaaa cttccgtaag tttatcgatt tggggtcagc
     1261 atgtgccata ataccaatct cttttggaag ctttataaca tcttcaaagg gcataacact
     1321 atgaaccggt ggcacctctt tgcgtaagca ttgctttgaa gcttcgcttg caattaactg
     1381 ccagcgttca acctttttat cttctttgcc agaaacatca ggtatggtat gctgcgtaac
     1441 aactggaatt atttgtttta ctccaatttc aacagctttc tggataacaa ggtcaaactt
     1501 tttgcccttt aatagtgcag cacataatgt acagtggaca ttgatatcag gatagttaag
     1561 tttctctgtt attgttgcaa ctatctttcc atcttctatt tgtgtgatgg ttgcatggta
     1621 ttgcataccg ttattatcaa ttatctgta
//
LOCUS       DAOSZP010000533         1645 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_54954_length_1645_cov_3.014465, whole genome shotgun sequence.
ACCESSION   DAOSZP010000533 DAOSZP010000000
VERSION     DAOSZP010000533.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1645
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54954_length_1645_cov_3.014465"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /locus_tag="PK348_10140"
     CDS             <1..556
                     /locus_tag="PK348_10140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HXK66617.1"
                     /translation="ACNDSGIHVFPWVMDFYKDLSVFDSSCTHSLEEYESYFGKGYVA
                     SIPGRYWIHYGGRALKLFKGYYRTKLLEEILASSPCREITNTSHFHFDVYGNYIPGLC
                     SGLAIQADDIGKEIDSITYPFITTLYSEGVAGLYRLAQQEGFVADSSYLNKCDLCLHI
                     RTFFVTQKKMNTPELQPVEFYYNT"
     gene            575..1261
                     /locus_tag="PK348_10145"
     CDS             575..1261
                     /locus_tag="PK348_10145"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HXK66618.1"
                     /translation="MKITTVIIRLLVLASPLLLNDLYLPLVPKDATTLSIVLDVLVYI
                     GWQTTLLYVAYEAGWFSWNSLGINLTQVKQWLWGVVLFVCVFVAYTGITIAFMFAKKY
                     YGITVESFWYFPLPQWHGVYVFLYVLYLSITAGIYEEIIYRGIAIHQLKNLTSNTGIL
                     VIVSTLLFVAIHWSMGPGTWMEAGLWGALWAYLYIKTHSLLPIMIAHVLYDVASIYGL
                     HAAIAHSIGL"
BASE COUNT          460 a          272 c          321 g          592 t
ORIGIN      
        1 cgcctgtaat gatagcggga tacatgtctt tccgtgggta atggattttt ataaggattt
       61 atcggtgttc gatagttcct gcactcattc tctggaagaa tatgaatcgt attttggtaa
      121 aggatatgtt gcgtcaatcc ctggcaggta ctggatacac tatggtggca gggcattaaa
      181 acttttcaaa ggttactatc gcacaaaact gcttgaagaa atacttgcaa gctcgccatg
      241 tcgtgagatt acaaatacca gtcatttcca ttttgatgtg tatggcaatt atattcctgg
      301 tctttgcagt gggcttgcaa tacaggcgga tgacattggt aaagaaattg acagcattac
      361 atatcctttt ataacaacgc tatactctga aggtgttgct ggattatata gattggcgca
      421 gcaggaaggt tttgttgctg acagtagtta tttaaataaa tgtgatttgt gcttgcatat
      481 tcgcacattt tttgttacac agaaaaagat gaacactcct gaattgcagc ctgttgaatt
      541 ttattataac acataattgt taaggaatcc aatcatgaaa ataacaactg ttattattcg
      601 ccttcttgtg ctggcatccc cgcttttgct caatgacctg tacctgccgc ttgtgccaaa
      661 agatgcaaca acgctttcta tagtacttga tgtgctggtg tacattggct ggcagactac
      721 actattatat gtggcctatg aggctgggtg gttttcatgg aacagtttag gtattaatct
      781 aacgcaggtc aagcaatggt tatggggtgt tgttcttttt gtctgtgtgt ttgttgccta
      841 cactggtatt accattgcat tcatgtttgc aaaaaaatat tacggcatta cggttgaatc
      901 attctggtat tttccccttc cccaatggca tggtgtgtat gtgtttttat atgtgctgta
      961 tctttcaata actgctggca tctatgagga aatcatttat cgtggcattg ccatacatca
     1021 gctaaaaaac cttacatcaa atacaggcat acttgtgatt gtttcaacgc ttttgtttgt
     1081 tgccattcac tggtcaatgg gtcctggtac ctggatggaa gctggtttat ggggagcgct
     1141 gtgggcttac ctgtacataa aaacgcattc gcttttgcca attatgattg cacacgtctt
     1201 atatgatgtt gcatctatat acggattaca tgcggcgata gctcatagta ttggtctttg
     1261 atatcgtaac atatcactta gacatctcgg aaagttttac tgttaactct tcaagagttt
     1321 tcatagaact aacaagattt ttatcatgct ctaatacgtc atgcattatg ttattcaggt
     1381 aataaattgc tttaacaatc tctactgcag tatttttacg ttcatcgatt gagttgttaa
     1441 tttttgataa gattttttaa tggtattcaa attactatca tcaaaattgt tttattaaca
     1501 tactatcgaa taagtattaa taactttcgc ttaaaataaa tattgtggta ataaatattt
     1561 gatatgaatt atagaaaaac ttgcgctatt attttataat tgtaattgtt gattaaaaat
     1621 aatattaaaa cgttttcttg tttaa
//
LOCUS       DAOSZP010000534         1644 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_55004_length_1644_cov_2.381372, whole genome shotgun sequence.
ACCESSION   DAOSZP010000534 DAOSZP010000000
VERSION     DAOSZP010000534.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1644)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1644)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1644
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55004_length_1644_cov_2.381372"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1105)
                     /locus_tag="PK348_10150"
     CDS             complement(<1..1105)
                     /locus_tag="PK348_10150"
                     /inference="COORDINATES: protein motif:HMM:NF024994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HXK66619.1"
                     /translation="MLDSLFNPKSVAVVGASEIPFKWGTYISSNILDGKFKGRYYPVN
                     PNILEVFGFKTYPSIRELPEAVDLVFITTPAKTVMQILQDCITKGIKNIVMITSGFSE
                     TGAEGTQMEKEIISLAKNHGLNIVGPNTMGIVNTHCSLYATGAITRPKPGGISIIAQS
                     GNLGYQIMEWAESEDIGIAKFVGSGNEAVLKIEDYLKYFKTDSQTKVILMYVEGVDDG
                     RVFVEIAKETSLHKPIIVLKAGRTEIGSKAAASHTGAMAGSFSIFKGVIQQTGIVLAE
                     SPRELLTLSAAFDSFPLPKGNRIGIITLGGGWGVVTADECAERGLVIPHLPQSIKEEL
                     DKRLPPFWSRNNPVDLVGQPDFQLFKDAVELMVA"
     gene            complement(1208..>1644)
                     /locus_tag="PK348_10155"
     CDS             complement(1208..>1644)
                     /locus_tag="PK348_10155"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013638957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HXK66620.1"
                     /translation="VPETHLIPLVIRAVLDPEYTVTIFGTDYNTPDGTCIRDYIHVND
                     LGEAHILALEKLLESGSSDVFNLGNGKGFSVKEVVDSVRRVTGKEFSVREGERRPGDP
                     AILVASSQKAKEKLHWKPVYTDIDSIVATAWKWELQRKRKGY"
BASE COUNT          473 a          373 c          281 g          517 t
ORIGIN      
        1 gagccaccat aagctcaact gcatctttga ataactgaaa atcaggctgg ccgactaaat
       61 ccacaggatt gtttctgctc cagaatggcg gcaaccgctt atcaagttct tcctttattg
      121 attgaggcaa atggggaata acaagacctc gctcagcaca ttcatccgca gtaacaacgc
      181 cccatccacc acctaacgtg atgatgccaa ttctattacc tttaggcagt ggaaacgaat
      241 caaatgctgc agaaagtgtt aataattcac ggggcgattc agctaatact atacccgttt
      301 gctgaattac acctttgaaa atggaaaagg agccagccat tgcgccagta tgtgatgctg
      361 cagctttgct gccaatttcg gttcttcctg ctttgagaac aatgattggt ttgtgaagcg
      421 atgtttcctt agctatctca acaaatactc tgccgtcatc aaccccttct acatacataa
      481 gtatgacttt tgtttgacta tcggttttaa aatatttaag gtaatcctca atttttaata
      541 cagcttcatt acctgaaccc acaaatttag ctattccaat atcttcactt tctgcccact
      601 ccataatctg ataccctaaa ttgccacttt gagcaataat tgaaatacca cctggctttg
      661 gacgggtaat agcccctgta gcatatagag aacagtgagt attgacaata cccattgtat
      721 ttggcccaac aatatttagg ccatgatttt ttgccagact gataatttct ttttccatct
      781 gagttccttc agctccagtt tcactgaagc cagaggttat catgactatg tttttaattc
      841 cttttgttat gcaatcctgc aaaatctgca taacagtttt tgctggagta gtaataaaaa
      901 ctaagtcaac tgcttctgga agctcacgta ttgaaggata tgttttaaat ccaaagactt
      961 ccagtatatt gggatttacc ggatagtatc gccctttaaa tttgccatca agtatattgc
     1021 ttgaaatata cgtaccccat ttaaaaggga tttctgaagc cccaactaca gcaactgatt
     1081 ttggattaaa caggctatca agcatcattt acctcttgga ttattatgta tgtatagcat
     1141 tattggttaa ttagtaagac atatatatgt atgtcaacga ttttgcacta attttttagt
     1201 acactcgtta ataccctttt cgcttcctct gcaattccca cttccatgca gttgcaacaa
     1261 tgctatctat atcagtgtac actggcttcc agtgtaactt ttcctttgct ttctgtgaag
     1321 atgcaaccag aattgcagga tcacctggcc ttcgttctcc ttcccttaca gaaaattctt
     1381 ttcctgtgac acggcgcact gaatcaacga cctctttgac actgaacccc ttcccgttac
     1441 caagattaaa cacatcgctt gaaccggatt ccagtagttt ttccagtgca agaatatgag
     1501 cttcacccag atcatttaca tgaatataat cacgtataca ggtcccatcg ggcgtattat
     1561 aatcagtacc aaagatagta actgtatact ctggatcaag tacagcacgg ataaccagtg
     1621 gaataagatg tgtttccggt acat
//
LOCUS       DAOSZP010000535         1640 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_55191_length_1640_cov_3.394322, whole genome shotgun sequence.
ACCESSION   DAOSZP010000535 DAOSZP010000000
VERSION     DAOSZP010000535.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1640
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55191_length_1640_cov_3.394322"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(56..337)
                     /gene="cas2"
                     /locus_tag="PK348_10160"
     CDS             complement(56..337)
                     /gene="cas2"
                     /locus_tag="PK348_10160"
                     /inference="COORDINATES: protein motif:HMM:TIGR01573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HXK66621.1"
                     /translation="METWLVIYDIRNEKRLRRIAKLMERYGVRVQKSVFETMCDEHTI
                     QRMRHEAKMILEKEDSLIVIPLCAKCWQKKIQYGVKTEGIGEYKKFQVL"
     gene            complement(373..597)
                     /gene="cas2"
                     /locus_tag="PK348_10165"
     CDS             complement(373..597)
                     /gene="cas2"
                     /locus_tag="PK348_10165"
                     /inference="COORDINATES: protein motif:HMM:TIGR01573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HXK66622.1"
                     /translation="MAKQLERMGIRVQYSFFNVELDDEMLDKLMQILLGLIDPKEDKI
                     YVYPLCTDCCKKVIIDGTGALLDLNTFIIL"
     gene            complement(645..>1640)
                     /gene="cas1"
                     /locus_tag="PK348_10170"
     CDS             complement(645..>1640)
                     /gene="cas1"
                     /locus_tag="PK348_10170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas1"
                     /protein_id="HXK66623.1"
                     /translation="DGTVSKFFPHNTQRVFIIGNIEITPEAFKMLMHNKIEVVFLNKN
                     GLFNGKLVFDDSKNVLLRKKQYDKLNNDAFILGWCKNIIEGKIRNQLVFANRIKRKHK
                     ETTQLQDYVNSMKDIIEKLETATTTNEARGYEGAASRMYFGAIKYAILPHWAQFNGRS
                     MNPPRDNVNAVLSFTYTLLNHLIESYILSEGIDTYVGYLHTVEYGRKSLIFDLMEEFR
                     SPVCDTLTVTLFNLGVLNPDDFRTIDFSSSDDEYPLEPMEEEEDNVSTRKGVLLTKDG
                     LKKVIEKFEAKLEETYYYMPLQRQLSYRKIIAQQVAHCKRVINDEESQYKPFEVK"
BASE COUNT          471 a          340 c          234 g          595 t
ORIGIN      
        1 cttaggttgg cggcatcaaa atatgcctta ttttatacag ttttcaaaga actgttcata
       61 aaacctgaaa ttttttatac tcaccaattc cttcggtctt tacgccatac tgaatcttct
      121 tttgccagca ctttgcacac aacggaataa caattaatga atcttctttt tcaaggatca
      181 tttttgcttc atggcgcatt ctctgtattg tgtgctcatc acacattgtt tcaaagaccg
      241 acttctgcac tcgtacaccg tagcgttcca ttagttttgc aatacggcgt aatcgctttt
      301 cattccgaat gtcgtatatt accaaccagg tttccatact atcatactat aattcatttg
      361 tcaataacta tctcataaaa ttataaatgt atttaaatct aacagtgcgc ccgttccatc
      421 tatgataacc tttttacaac aatcagtaca tagcggatac acatatattt tatcttcttt
      481 gggatctatc aatcccagta gtatctgcat cagcttatca agcatttcat catccagttc
      541 tacattaaag aaagaatact gtacgcgtat gcccatacgc tccaattgct ttgccacacg
      601 agttaacctc ttatgatcac aaatatcata acatatcaaa tatttcattt tacctcaaac
      661 ggtttgtact ggctttcttc atcatttata acacgcttgc agtgtgctac ctgctgcgct
      721 attattttac ggtacgatag ttgtctttgc aatggcatat agtaatatgt ttcttctaat
      781 tttgcttcaa atttttctat tacctttttc aaaccatcct ttgttaaaag tacgccttta
      841 cgcgttgaaa cattatcctc ttcttcttcc attggttcca gcggatattc atcatcacta
      901 cttgaaaaat ctattgtacg gaaatcatca ggattaagta cacctaaatt aaacagtgtt
      961 actgttaagg tatcgcatac tggtgatcgg aattcttcca ttaaatcaaa gatcaacgat
     1021 ttgcggccat attcaactgt atgcaaataa cccacataag tatctatacc ttctgaaagt
     1081 atgtagcttt caattaaatg atttaataat gtataggtaa aacttaacac tgcattgaca
     1141 ttatcgcgtg gtgggttcat acttcggcca ttaaattgtg cccagtgtgg tagtattgca
     1201 tatttaattg caccaaaata cattctggat gcagcacctt catagccacg cgcctcattg
     1261 gtggtggttg cagtttcaag cttttctata atatccttca ttgagttgac ataatcctgt
     1321 aattgtgtgg tttctttatg ctttcgtttt atacgattgg caaataccag ctgattgcgt
     1381 atcttacctt caattatatt tttgcaccac ccaagtataa atgcatcgtt attgagttta
     1441 tcgtactgct ttttacgcaa taatacattt ttgctatcat caaataccag cttaccatta
     1501 aataatccat ttttgtttaa aaacaccact tcaattttat tatgcataag catcttaaat
     1561 gcttcaggag ttatttcaat atttccaatg atgaatactc gttgtgtatt atgggggaaa
     1621 aattttgaca ctgttccatc
//
LOCUS       DAOSZP010000536         1640 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_55195_length_1640_cov_3.038486, whole genome shotgun sequence.
ACCESSION   DAOSZP010000536 DAOSZP010000000
VERSION     DAOSZP010000536.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1640
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55195_length_1640_cov_3.038486"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..721
                     /locus_tag="PK348_10175"
     CDS             <1..721
                     /locus_tag="PK348_10175"
                     /inference="COORDINATES: protein motif:HMM:NF016612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HXK66624.1"
                     /translation="TFQVIKINEIFFLPIPFEVTYESGNRISKEVKQLSATPIIPIVI
                     SCANGYTGYCTTPEEYSMQRYEGGHTLYGPNTQPFLALQYAQLTTTIAKTLGDSNWSK
                     TYSMKSASFLKKYPHPANPVRKAVKQPVYVVNPGIDEPCVSFLWEDVPPDQLEFNKPI
                     VAIEYSLQNDFKPFTQNEIIVDDTGYDIAVIFTGTITKEGMAIYEARWYNPPLNSNYH
                     FRFVILPREHLNILHSEQFSW"
     gene            complement(1000..1446)
                     /locus_tag="PK348_10180"
     CDS             complement(1000..1446)
                     /locus_tag="PK348_10180"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HXK66625.1"
                     /translation="MWKSIALLFIASHITVDGVIDCYDTDIRKKLKTISQGGFTVAIE
                     TSATDTGLYTALAALKPRGRMVMAGINFYGQPVPTLSLIAKEITVLGSMGYSIDEFDD
                     VIENMAEKKLALEALVTHSVSLEVVPVVFNNLAQKSIEAIKVVVQP"
     gene            complement(1431..>1640)
                     /locus_tag="PK348_10185"
     CDS             complement(1431..>1640)
                     /locus_tag="PK348_10185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66626.1"
                     /translation="IREKWGAESVVFGSATTRKMAPYLNKFLAINKEDAFIEPAAVAL
                     HVVNLAGVRSSDTVWCIMYCDCGSR"
BASE COUNT          532 a          342 c          246 g          520 t
ORIGIN      
        1 cacatttcag gtaatcaaga taaatgaaat attttttctt cccataccat ttgaagtaac
       61 atatgaatca ggcaaccgta tcagtaagga ggtaaagcaa ttatctgcca cacctattat
      121 cccaatcgtc ataagttgtg ccaatggata cactgggtat tgcaccacac ctgaagagta
      181 ttccatgcag cgttacgaag gcggacacac cctgtatggg ccaaatactc aaccttttct
      241 tgcattgcag tatgctcagt taacaacaac tatcgccaaa acattgggcg atagtaactg
      301 gtcaaaaacc tacagtatga aaagtgcctc attcttaaaa aaatatccac accctgccaa
      361 tcctgtacgt aaagcagtaa aacaacctgt ttatgttgta aatccaggga tagatgaacc
      421 ctgcgtttct tttttgtggg aagatgtacc tcctgatcaa ttagaattca acaaaccaat
      481 tgttgctatt gaatatagtt tgcagaatga ttttaagcca ttcactcaaa atgaaattat
      541 agtggatgat actggctatg acatagcagt gatatttaca ggcacaataa caaaagaagg
      601 gatggcaatc tatgaagcac gctggtataa tcctccactt aattcaaatt accactttag
      661 atttgtaatt ctgccacgag aacatctaaa tatactacat tcagaacaat tttcctggta
      721 gtatataaaa gcctcatttt caatatgatt atgcttatcc agttatttga ctattgtaat
      781 ccgcttactg gtcatatgct ggggctttca tatcattaat tttccttaat acacaaccat
      841 tacaatatct gctctttttt atctttaaat gagcacttaa agtaatgagt aattgcaaca
      901 atatttccca tcattttttt acaaagctta cctaactttt tttaaatctt atcttctttt
      961 tcatttcatt catgaatagc accttatgca aaaatattat tatggctgta caaccacctt
     1021 gatagcctca attgattttt gagcaagatt gttgaaaact actggcacaa cttcaagcga
     1081 tacactatga gtaacaagtg cttctaaagc taattttttc tcagccatat tttcaataac
     1141 atcatcaaac tcatcaatac tatatcccat tgaacctaac actgtaattt cttttgcaat
     1201 taaagagagt gttggaaccg gctgaccata aaagtttata ccagccataa ccatccgtcc
     1261 tctgggttta agagctgcta gtgctgtata aagtccagta tccgttgctg atgtttcaat
     1321 agcaaccgta aatcctccct gtgaaatggt tttcaatttt ttccgtatat cagtatcata
     1381 acaatcaata acaccatcaa ctgtgatatg ggatgctata aataataaag ctatcgactt
     1441 ccacaatcac aatacatgat gcaccaaaca gtatcagaag accttacacc tgctagatta
     1501 actacatgga gagcaactgc tgccggttca atgaaagcat cttctttatt aatggccaaa
     1561 aatttattta aatatggtgc catttttcgt gtggttgccg agccaaacac tactgattct
     1621 gcaccccatt tttcccttat
//
LOCUS       DAOSZP010000537         1639 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_55231_length_1639_cov_4.409722, whole genome shotgun sequence.
ACCESSION   DAOSZP010000537 DAOSZP010000000
VERSION     DAOSZP010000537.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55231_length_1639_cov_4.409722"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..994)
                     /locus_tag="PK348_10190"
     CDS             complement(<1..994)
                     /locus_tag="PK348_10190"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HXK66627.1"
                     /translation="MDITIQKRIEQWQNPPFDTSTIQEIKQLVAQNNEKELYERFYTT
                     LEFGTGGLRGIIGAGTNRMNIYTVGMATQGLANYIVHQGNAAKGVVIAYDSRRMSDVF
                     SKEAASILAGNGIKVYLFKDIMPTPICSFAIRHYNATAGIVITASHNPPEYNGYKVYW
                     EDGGQVVPPQDKEIIREVSKIQDITQIKKMNFDEAIAHGIILLVGDEIIESYAQKLSQ
                     VRFKSDELSPIHIVYTPLHGTGYRIVPQILKTSGFPNVHIVEQQAIPDGNFPTVKSPN
                     PEEPEALTQAIELAQQINADIVLATDPDADRMGVAFKDDNGQYMLINGNQIGSML"
     gene            1291..>1639
                     /locus_tag="PK348_10195"
     CDS             1291..>1639
                     /locus_tag="PK348_10195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66628.1"
                     /translation="MYIETTTCISYKHLEILKFYAEKKNMSLRTFVSSIISFAALCEK
                     GDIQYFKQIQYRTRNEGEWKRLHLVLYNDEYEFFLDVKKLWKMSLAKVIAYCLDNVLY
                     EFLEFLTKAEEDED"
BASE COUNT          491 a          262 c          326 g          560 t
ORIGIN      
        1 aaagcatgct gcctatctga tttccattaa taagcatata ttgtccattg tcatctttaa
       61 atgctacgcc catgcggtca gcatcgggat cagttgctaa cactatatcg gcattgattt
      121 gttgtgcaag ttcaatggcc tgtgtaagcg cttcaggttc ttcaggattg ggtgatttga
      181 cagtggggaa gttgccatca gggattgcct gttgttcaac tatatgaaca ttggggaacc
      241 cagatgtttt gagtatttga ggaacaatac ggtaaccagt accatgtaat ggtgtgtata
      301 caatgtgtat tggcgatagt tcatcggatt tgaatcgtac ctgtgaaagt ttctgggcat
      361 agctttcaat gatttcatcg ccaactaaca atataatgcc atgagctatc gcttcatcaa
      421 aattcatctt cttaatttgt gtgatgtcct gtatttttga aacttcacgt attatttctt
      481 tatcctgggg cgggacaacc tgaccgccat cttcccagta taccttatat ccgttatatt
      541 ctggtgggtt atgactggct gtgataacaa tacctgctgt ggcattgtaa tggcgtattg
      601 caaatgagca gattggtgtg ggcatgatat ccttgaaaag atatacctta atcccattgc
      661 ccgctaatat agaggctgct tcttttgaga atacatctga catcctgcgt gaatcatatg
      721 ctataactac accttttgca gcatttccct ggtgtacaat gtagtttgcc aggccctgtg
      781 ttgccatgcc aacggtgtaa atgttcattc ggttggtgcc agcaccaatg attccacgaa
      841 gtccgcctgt gccaaactca agtgtggtat aaaagcgttc gtacagttct ttttcattat
      901 tttgtgcaac aagttgtttt atctcctgta tagtgctggt atcaaatggt ggattttgcc
      961 attgttctat tcgtttttga atggtgatgt ccatggtaac tatctcctta taaaacggat
     1021 tatctttgag ctattttaaa ctataatacc tgcaaggtca atagcataat tatggatgta
     1081 aaggatgtac aaaaattatt tgttattatc actgtggcaa taaaaaaatt ctaaatgttt
     1141 tagtaaaaaa ttaatataga tttcagttga cacctaatgt tagtttatat ttgatttttc
     1201 aaaatattat tttatgcttg aaaaattatt ataaattatt acgataaaat caaatataaa
     1261 aaataaaaac tactttggaa gggaaagcat atgtatattg aaaccaccac gtgcatttca
     1321 tacaaacatc ttgaaatatt gaaattttat gcagaaaaga aaaacatgtc gcttaggaca
     1381 tttgtatcct caataataag ttttgctgct ttgtgtgaaa aaggtgatat acagtacttt
     1441 aaacaaatac aatacagaac acgtaacgaa ggggagtgga agagattgca tctggtatta
     1501 tataatgatg agtatgagtt ttttcttgat gtgaagaagc tatggaagat gtcattggca
     1561 aaggttattg catattgtct ggataatgta ttgtatgaat ttttggaatt tttaacaaaa
     1621 gctgaagaag atgaagatt
//
LOCUS       DAOSZP010000538         1635 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_55415_length_1635_cov_3.756329, whole genome shotgun sequence.
ACCESSION   DAOSZP010000538 DAOSZP010000000
VERSION     DAOSZP010000538.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1635
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55415_length_1635_cov_3.756329"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /locus_tag="PK348_10200"
     CDS             <1..411
                     /locus_tag="PK348_10200"
                     /inference="COORDINATES: protein motif:HMM:NF025684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4384 domain-containing protein"
                     /protein_id="HXK66629.1"
                     /translation="PNDGLKIGQSVKFKFRANKSGYVTIVDIQPTGEVVILFPNQFNM
                     NNYITADTVYSIPSETDGFELTVTEPAGEDTITLFFTEKKVDWLDMKKLQGEGFQSVE
                     PAERFAVTRGFSVTATQLNTKQWESVVINVLVKK"
     gene            423..815
                     /locus_tag="PK348_10205"
     CDS             423..815
                     /locus_tag="PK348_10205"
                     /inference="COORDINATES: protein motif:HMM:NF024637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HXK66630.1"
                     /translation="MKSIKPGRMTSFQGFVGSIIAIVFGIFWLFLVTSFMFENEDIPQ
                     AIFFAFAFFGVVFIVIGIVNAVVEYKNAFSKNRYSLLDVVDSSEEHDPFHFKNNTATI
                     NTINYCPHCGASIQKEYNFCPGCGRKLK"
     gene            835..1620
                     /locus_tag="PK348_10210"
     CDS             835..1620
                     /locus_tag="PK348_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009616294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HXK66631.1"
                     /translation="MTQKITVEKKGHILLIGLNRPEKRNAFDVDMYWDLAKAYGTLDA
                     DDELRCGVLFAHGEHFTGGLDLVQWVEPFSRGEYPALPSDSKDPFGIDEDNRVSKPIV
                     AAVQGICFTVGWELMLATDVRVAATNVRLAQIEVKRGIYPVGGATVRLFEEIGWGNAM
                     RYLLTGDEMNAQEAYRLGLVQELVEPGKQLERAIEIAEVIAKQAPLGVRAILRSARLS
                     RVEGAKAALARLLPDLLPIMKSEDAQEGVKAFIERREANFKGR"
BASE COUNT          464 a          259 c          416 g          496 t
ORIGIN      
        1 ccaaacgatg gtttgaaaat tgggcaatca gtaaagttta agttcagggc aaataaatcg
       61 ggatatgtta ccattgttga tattcagcca acaggtgagg ttgttatttt gtttcccaat
      121 cagtttaaca tgaataatta tattactgct gatacagtat actctattcc ttcagaaact
      181 gatgggtttg agttaacggt tacagaacca gcaggtgagg ataccattac gttatttttt
      241 actgaaaaaa aagtggactg gctggatatg aaaaagttac agggtgaagg ctttcagagt
      301 gtggagcctg ctgaacgttt tgctgtaacg agaggatttt cagtaactgc aacacagctt
      361 aatactaagc aatgggaaag tgttgttatt aatgttttag tgaaaaaata agaggtatta
      421 gtatgaaaag tatcaaaccg gggaggatga cttcatttca gggttttgta ggttcaatta
      481 ttgctattgt gtttggtata ttctggttgt ttcttgtaac ttcttttatg tttgaaaatg
      541 aagatattcc acaagctatt ttttttgcgt ttgcattttt tggtgttgtt tttattgtta
      601 taggcattgt gaatgcggtt gttgaatata agaatgcgtt tagcaaaaac cgttactcgt
      661 tattagatgt tgtcgatagt tctgaagaac atgacccatt ccattttaaa aataatactg
      721 ctacaataaa taccataaac tactgcccac actgtggtgc atcaattcaa aaagagtata
      781 acttttgccc aggttgtggg aggaaattaa aataatttta gaataggggt ttttatgaca
      841 caaaaaataa cagttgaaaa gaaaggacac atattactga tcgggctaaa ccgccctgaa
      901 aagcgcaatg cttttgacgt agatatgtac tgggatctgg caaaagcgta tggcaccctt
      961 gatgctgatg atgaattgcg ctgcggtgtt ttgtttgccc atggcgaaca tttcaccgga
     1021 gggcttgatc tggtacaatg ggttgagccg ttttccaggg gggagtatcc cgcacttcct
     1081 tcggacagca aggatccatt tggcattgat gaggataata gggtatcaaa gccaattgtt
     1141 gctgcagtgc agggtatatg ctttacagtg gggtgggagc ttatgcttgc aactgatgtg
     1201 cgtgttgctg ctactaatgt gcgcttggct cagattgagg taaagcgagg tatttatcct
     1261 gttggcggtg caacggtaag gctttttgag gagataggct ggggcaatgc catgcgatat
     1321 ttactcaccg gtgatgagat gaatgcccag gaagcctacc gccttgggct ggtacaggaa
     1381 ctggttgagc cgggaaagca gttagagcgt gcaattgaaa ttgctgaagt tatagccaaa
     1441 caggcaccat tgggggtcag agccatatta cgttcggcac ggctatctcg tgtggaaggg
     1501 gctaaagcag cactggcgcg gttgctacct gaccttttgc caataatgaa aagtgaggat
     1561 gcacaggaag gggtcaaagc ttttattgag cgccgcgaag ctaactttaa ggggagatag
     1621 ttatatttat ataat
//
LOCUS       DAOSZP010000539         1634 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_55471_length_1634_cov_2.962001, whole genome shotgun sequence.
ACCESSION   DAOSZP010000539 DAOSZP010000000
VERSION     DAOSZP010000539.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1634
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55471_length_1634_cov_2.962001"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1061)
                     /locus_tag="PK348_10215"
     CDS             complement(<1..1061)
                     /locus_tag="PK348_10215"
                     /inference="COORDINATES: protein motif:HMM:NF014820.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HXK66632.1"
                     /translation="MEAAIIGVGMTTIEKSKVGETYADLAWEAVNKALNDAGLGIDDI
                     DNVITTSSDFWDGRTISCMAVGDASGVRYKNCSAVEGDGTFGAAYALARSLSGDYKTT
                     LVTALSKGSESISSIITNAAFDPIYERGLGMDMISACAMQARAYMHATGTTPEQLALV
                     SCKNHKNAKDNPYAQLPMDITVKDVLSSEMIADPLHKLDCSPVSDGCCAIIIANADFV
                     KKIKAKPVWIKGVAFCSDAYFMGDRDLSKAKALEEAAKKAYSMAGITNPQNEINVAEI
                     YDAFTYQELMWIEGLGLAEYGKAGLMLEKGIFNRDGKLPVNPSGGLLSGHPVIAAGLY
                     RIAEVVLQLRGEAGGRQVNA"
     gene            complement(1072..1533)
                     /locus_tag="PK348_10220"
     CDS             complement(1072..1533)
                     /locus_tag="PK348_10220"
                     /inference="COORDINATES: protein motif:HMM:NF013920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HXK66633.1"
                     /translation="MEDIFKEIEPQVYKSKISVPYQWWAGETASKFFITLRDEKKILG
                     TECPQCAKVFCPPKKTCPFCYVDTKNWKEVGPKGKVITYTVVYRQLAALKKNVPVAYG
                     LIQLEGSANAMLHYIEGIEPEKISKGMEVEAVFADEKSASIHAISYFRPVK"
BASE COUNT          442 a          408 c          262 g          522 t
ORIGIN      
        1 gcgttaacct gccgcccacc tgcttcacca cgcaattgca acacaacctc agcaattcgg
       61 tacaaacctg ctgcaatgac ggggtgacca cttaataatc cacctgatgg atttactggc
      121 agcttaccat cccggttaaa tatgcctttt tcaagcatca aacctgcctt tccatattct
      181 gccaaaccca gtccttcaat ccacataagt tcctgatagg taaatgcatc atatatttct
      241 gccacattaa tttcattttg cggatttgta atacctgcca ttgaatatgc tttctttgca
      301 gcttcttcca gtgctttagc ttttgataaa tctctatcgc ccataaaata tgcatcactg
      361 cagaatgcta cacctttaat ccatactggc ttagctttaa tctttttcac aaaatcggca
      421 ttagctatga taattgcaca acacccatca gaaaccggtg agcaatctaa tttatgcagc
      481 ggatctgcaa tcatttctga acttaataca tctttcaccg tgatatccat tggcaactgt
      541 gcatagggat tatcctttgc atttttgtga tttttgcatg aaaccagtgc aagctgttcc
      601 ggtgttgtcc cagtagcatg catatatgcc cttgcctgca ttgcacatgc cgaaatcata
      661 tccattccta gcccacgctc atatatagga tcaaacgccg cattggtaat gatgctggag
      721 atactctcag atcctttact aagtgccgtt accagtgttg ttttataatc accggaaagt
      781 gagcgtgcaa gtgcatatgc tgccccaaat gttccatcac cttcaacagc agagcagttt
      841 ttatatctaa ccccactggc atctcctact gccatacacg atatggttct gccgtcccag
      901 aaatcactgg aagtggtaat gacattatca atatcatcaa ttcctaaacc ggcatcattc
      961 aatgctttgt taacggcttc ccaggccagg tcagcatacg tttcaccaac cttgcttttt
     1021 tctattgtgg tcataccaac accaattatt gcagcttcca ttgctaacct cctattttac
     1081 cggcctgaag taagaaatgg catgtataga agcacttttt tcatcagcaa aaacagcttc
     1141 tacttccatt ccttttgaaa tcttttcagg ttcaattcct tcaatatagt gcaacattgc
     1201 gttggctgaa ccctcaagct gtatgagccc atatgcaaca ggcacatttt tctttaacgc
     1261 tgcaagttgc ctgtatacta cagtatatgt aataactttc cctttgggcc caacctcttt
     1321 ccagttcttt gtatccacat agcaaaatgg gcatgttttc ttcggaggac agaacacttt
     1381 tgcacactga gggcactcag tccccagtat tttcttctca tcacgaaggg ttatgaaaaa
     1441 tttagatgct gtttctcctg cccaccattg atagggaaca cttatcttgc ttttatatac
     1501 ctgtggttca atttccttaa agatatcttc cataactatc tcctataaaa agtcaaaata
     1561 ttttacagcc ctattcaaat attgtaaatc tatagaataa tcaggttatg ctaattgctt
     1621 ttcttgcatg gacc
//
LOCUS       DAOSZP010000540         1633 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_55513_length_1633_cov_3.503802, whole genome shotgun sequence.
ACCESSION   DAOSZP010000540 DAOSZP010000000
VERSION     DAOSZP010000540.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1633
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55513_length_1633_cov_3.503802"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            346..>1633
                     /locus_tag="PK348_10225"
     CDS             346..>1633
                     /locus_tag="PK348_10225"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HXK66634.1"
                     /translation="MAILNISQLVDRFEKNEPVSLSFYYPNENISKTLNALLAKILSN
                     QDKIFLLDMMITILREIIVNATKANAKRAYFKKINLNINDPECYQKGIENFKKDIVGQ
                     LDTLEKDLKDNDLKVTITIKKQDDGIRFSITNPTPIHPVELSRIKTRIEKAKQYLDFT
                     QAYEDVYDDTEGAGLGIVLVILLLKNAGVDPSNYKIGTDGKITQVLLNLPNQLRPEKL
                     TTTIKQQILEQIEGIPTFPDHIIQLLELCRRKDVEIKDITFSIMRDPALTTDELKLAN
                     SAGFVPGKRIDNLSEAVMTIGLKNLESLLMTISARKILDERFQKFEQIWDHCDKTASY
                     ARTIATVYRINTIVEQVSLCGLLHDLGKIVLLSVNLELTNWIADLVSDRKIRTSTVME
                     EISKGISHSGIGQLIAQKWQFPQYLVEAIQWHHSPLS"
BASE COUNT          587 a          304 c          287 g          455 t
ORIGIN      
        1 ccactggtct ttatacagca taaccaaaag ccgctgcaac aacctgtatg gaaaaagctt
       61 aatgacatat atacttcaat gcagcataaa ttggtaaaat aaagcgattc ttgtagcaat
      121 aataattcct aaaaaaaagt attttacatg tatattatac ttctggcgca ttcggcagat
      181 caataaatag atcatttatt aatttgaaaa aagttagaaa aaaataatga ctgttactct
      241 aatgataatt ccaaaaataa aatgattgta tttaactttg ctctatggta tattcttccg
      301 ataatgatat atatttgaag aattatccat attataagga atactatggc aatattaaat
      361 atatctcagc ttgtagatcg gtttgaaaaa aatgaaccgg taagcctttc attttattat
      421 cccaatgaaa atatatcaaa aaccctgaat gcactgcttg caaaaatcct ttccaaccag
      481 gataaaatat tcctgcttga tatgatgatt actattttgc gggaaataat cgttaatgcc
      541 accaaagcaa atgctaaaag ggcttatttc aagaaaatca atttaaacat taatgacccc
      601 gaatgttatc aaaaaggtat agagaacttc aaaaaagata ttgttgggca attagacaca
      661 ctggaaaagg atctgaagga taatgacttg aaagttacca taaccataaa aaagcaggat
      721 gatggaatac gattttcaat tactaaccct actcctatac atccggttga actatcacgc
      781 attaaaacgc gtattgaaaa agcaaagcag tatttagact ttacccaggc ctatgaagat
      841 gtatatgatg atactgaagg ggcaggcctt ggaatagtat tagtgatatt gcttttaaaa
      901 aatgcaggtg ttgacccatc aaattataaa ataggaactg atggcaagat aactcaggtg
      961 ttactcaatc tgcccaacca attacgacca gagaaactta caacaaccat aaaacagcag
     1021 atacttgaac aaattgaagg catccctacg ttccccgacc atatcattca gcttcttgaa
     1081 ctttgcagaa ggaaggatgt tgaaattaaa gacattacat tttccatcat gcgtgaccct
     1141 gcactcacta ccgatgagtt gaagcttgca aattcggctg gatttgtgcc tggcaaacgc
     1201 attgacaact taagtgaagc agtcatgaca attggtctaa aaaacctgga atcattgctt
     1261 atgacgataa gtgcccgaaa aatactggat gagcgcttcc agaaatttga acagatatgg
     1321 gaccactgcg ataaaacagc ttcttatgca cgaactatcg ccaccgtgta ccgaataaac
     1381 accatcgttg agcaggtttc attgtgtggc ctgctccatg atttaggcaa gatagtactt
     1441 ctttcagtta atctggagct taccaactgg atagctgacc ttgtatcaga ccgcaaaatt
     1501 cgtacgtcaa cagtaatgga agaaatttca aaaggcataa gccattcagg cattggacag
     1561 cttattgcac agaagtggca attcccacaa tatctggttg aggcaataca gtggcaccat
     1621 tcaccattgt cat
//
LOCUS       DAOSZP010000541         1633 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_55515_length_1633_cov_3.317490, whole genome shotgun sequence.
ACCESSION   DAOSZP010000541 DAOSZP010000000
VERSION     DAOSZP010000541.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1633
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55515_length_1633_cov_3.317490"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(92..1390)
                     /locus_tag="PK348_10230"
     CDS             complement(92..1390)
                     /locus_tag="PK348_10230"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HXK66635.1"
                     /translation="MDRITQHDTLIDTLGNCTIDSPLSIPKFISDSQRIAVDIEAEII
                     EKSLEKGNIPAFENAGPRKKIFFQPQNTRAAIVTCGGLCPGINNVIQGIVRMLTFQYG
                     VKTIFGIRYGFEGLIPHFGHSFIELTPQFVHDLHEKGGTVLGSSRGRQDEKEIVDTLV
                     AHAINILFVIGGDGSLRCAYDIYREVKKRNLPIAIVGIPKTIDNDIWFVDKTFGFSTA
                     VSTAAQAIYAAHAEAIGAKNGIGIVRVMGRDAGYIAAYVTLASNEVNYCFIPEVHFDL
                     EGAHGFLPHLEKRLYTASHAVIVVAEGAGQEYFDGPQQIDSSGNVKYGDIGLLLKDTI
                     TTYCKQKNIPVTIKYIDTSYLVRSIPASSDDAVYCIMLAQNAVHGAMAGKTGFVVGSW
                     HSYYTYIPLSLVTKKRKRIDPHGYLWSIVKQSTCQPDFFN"
BASE COUNT          525 a          315 c          289 g          504 t
ORIGIN      
        1 caaaagaaga gtaaacttat tggtagtcaa cctttctttt gtaagaaagg ttgcacttgt
       61 gacttgaatc caagaagaaa tattatttgc gttaattaaa aaaatcaggc tggcaggtgc
      121 tctgtttaac aattgaccag aggtatccat gtggatctat gcgcttacgc tttttcgtta
      181 ccagcgatag tgggatgtag gtatagtatg agtgccagct accaactaca aagccggttt
      241 tgccagccat agctccatgt accgcatttt gtgcaagcat tatacaatat actgcatcat
      301 cgcttgaggc agggatactt cgcaccagat agcttgtatc aatatatttg atagttactg
      361 gtatattttt ttgtttgcaa tatgtggtaa ttgtatcttt aagcaataaa ccgatatcac
      421 catattttac attaccggaa gaatctatct gttgcggtcc atcaaaatat tcctgacctg
      481 caccttcagc aactacaata acggcatgag aagcagtgta taaccgcttc tccagatgag
      541 gtaaaaagcc atgcgctcct tctaaatcaa agtgaacctc tggaataaag caatagttta
      601 cctcatttga tgctaatgta acatacgctg cgatataccc tgcatcacga cccataacac
      661 gtacaatacc aattccgttt ttagcaccaa ttgcttcagc atgcgctgca taaattgcct
      721 gtgcagcagt ggaaacggca gtggaaaatc caaaggtttt gtcaacaaac catatatcat
      781 tgtcaatagt tttcggtatg ccaacaattg ctatgggaag gtttcttttt tttacttcac
      841 gatatatatc ataggcacaa cgtaatgaac cgtctccacc aatgacaaaa agaatgttaa
      901 tagcatgagc tactagtgta tcaacaatct ccttctcatc ctgccttcct cgtgaagaac
      961 ccagtactgt cccacccttt tcgtgaagat catgtacaaa ttgaggtgtt aattcaataa
     1021 atgaatgacc aaaatgtggg ataagacctt caaagccgta ccgtatgcca aatattgttt
     1081 taaccccata ttggaatgtt agcatacgaa caataccctg aataacatta ttaatacccg
     1141 gacaaagacc tccacaggta acaatagctg ctcgggtgtt ttggggttgg aaaaatattt
     1201 ttttacgagg accagcattt tcaaatgcag gaatattccc cttttcaagc gatttttcaa
     1261 taatctcagc ttcgatatcc actgcaatac gctgcgaatc gcttataaat tttggtattg
     1321 aaagaggcga atcaatggta caattgccca acgtatctat gagggtatca tgctgtgtaa
     1381 tccgatccat acatctccat ttatatgttt ataataaaca atgttgcgat aataaataaa
     1441 ttacattttt atattcagtt tagaatgtca agatataaaa tgctaactat ttttatgata
     1501 aatagccaat aattgttatt gacaggtgaa aaaacagtaa ataggtataa aatataataa
     1561 ctgatatagt ctaaaatttg ttgcactttt atagtgattt gtaagtatca tagcactggt
     1621 atcaactgtg tac
//
LOCUS       DAOSZP010000542         1632 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_55578_length_1632_cov_1.828789, whole genome shotgun sequence.
ACCESSION   DAOSZP010000542 DAOSZP010000000
VERSION     DAOSZP010000542.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1632
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55578_length_1632_cov_1.828789"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="PK348_10235"
     CDS             <1..246
                     /locus_tag="PK348_10235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66636.1"
                     /translation="LNNAAAQTKLYPVVLTGSVLCKVDARQKPVKPGDLLVTSDTPGC
                     GMAASIDSFDKVGTVFAKALTALDDGIALIPVMIWKM"
     gene            complement(259..840)
                     /locus_tag="PK348_10240"
     CDS             complement(259..840)
                     /locus_tag="PK348_10240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66637.1"
                     /translation="MKKIVWIICIASFALYTTFTPAQQKAPQNTQPQSKSIADGYGNV
                     KWGSTYNDVKPNVLGRIAFTDEKRIIVSKDGELEYRYGFFYPEINQEANQQPKLFYVV
                     IQFPYISLDDMKKKMEEKYGPPTGESIKNNQGAYIWESESTSIIVWVDSYEKKPFCRK
                     ITYLSKVMAKDINAYHNLVFSKKEQEILKQLQP"
     gene            1105..>1632
                     /locus_tag="PK348_10245"
     CDS             1105..>1632
                     /locus_tag="PK348_10245"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HXK66638.1"
                     /translation="MEDHHEHSHTHTHQISSTGRFVLVVTLNVIITVAEYIGGIVSGS
                     LALISDAGHNLSDVASLILGYIGQKVSQQKPGKKYSFGLKRFEVLIALINAMTLLGIG
                     AYIIYEAIERYINVQPVNPVIMLPVAFIGLAGNVISMLLLYKSRDENLNLKAAFLHLL
                     YDAVSSVGVIIVGFVL"
BASE COUNT          459 a          299 c          314 g          560 t
ORIGIN      
        1 ttaaataatg cagcagcaca gacaaagctg tatccggtag tattaacagg ctcagtgttg
       61 tgcaaggtgg atgcacgcca aaagccagtt aaacctggtg accttttagt aacatcagat
      121 acaccaggct gcggtatggc tgcttcaatt gattcttttg ataaagtggg cacagtgttt
      181 gcaaaagcac tcactgcgct ggatgatggt atagctttga ttccggtaat gatctggaag
      241 atgtagctac taccgtattt atggttgaag ttgtttgagt atttcctgct cttttttgct
      301 gaaaacaagg ttgtgatagg cattgatatc ttttgccatt accttgctta agtacgttat
      361 cttgcggcag aagggctttt tctcataact atcgacccac acaataatag atgttgattc
      421 tgattcccag atataggcgc cctgattatt cttaattgat tcaccagttg gcggaccgta
      481 cttttcttcc atcttctttt tcatatcatc caatgaaata tatggaaact gaataaccac
      541 ataaaacaat tttggctgct ggtttgcttc ctgatttatt tcaggataaa aaaagccgta
      601 gcggtattct aattctccgt cttttgacac aataattcgc ttttcatcgg taaatgctat
      661 tcttcctaac acattgggtt ttacatcatt gtaggtacta ccccatttta cattcccgta
      721 accatcggca atagatttac tctgcggctg agtgttctgg ggcgctttct gctgtgcagg
      781 tgtaaaggtg gtatataatg caaaacttgc aatgcagatt atccaaacta tttttttcat
      841 agtactatcc ttcataccag tttatgtact gtttatacta tcggttaatc ttatgttaaa
      901 atatattata atttatgggc gatagttccg gtaatatgct tttgtatata tacattctgt
      961 aaagatgact attttgtatt acagtagtga ataaaaatcc aatccaggcg gagtaatatt
     1021 ataaaaataa ttcttgtaaa aatttttgca cagtaactat tgtatactat actatagtaa
     1081 ttacttctat attagggaaa agacatggaa gaccatcacg aacattctca tacacacaca
     1141 catcaaatca gttccaccgg acgatttgtt ctggttgtga cactgaatgt catcataaca
     1201 gttgcagaat acattggtgg catagtgtca ggaagccttg cgctcatctc ggatgcgggg
     1261 cataatcttt ccgatgttgc atcacttatt ttaggataca ttggtcaaaa agtgtcacaa
     1321 caaaaacccg gaaagaaata ttcctttggg ctcaaacgtt ttgaggtact catagcgctt
     1381 atcaatgcca tgacacttct tggtattggt gcttacatta tttatgaagc aattgaacgc
     1441 tatattaatg tacagccggt aaatccagtt attatgcttc ccgttgcgtt tattggcctt
     1501 gcaggtaatg ttatctcaat gctgctttta tataaaagca gggatgaaaa tcttaatttg
     1561 aaggctgcat ttttacatct tttgtatgat gcagtatcct cggttggcgt tattattgtg
     1621 ggttttgtac tg
//
LOCUS       DAOSZP010000543         1630 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_55645_length_1630_cov_3.869841, whole genome shotgun sequence.
ACCESSION   DAOSZP010000543 DAOSZP010000000
VERSION     DAOSZP010000543.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55645_length_1630_cov_3.869841"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="PK348_10250"
     CDS             complement(<1..212)
                     /locus_tag="PK348_10250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66639.1"
                     /translation="MKYIIFFIVILHGLIHVLGFLKSLGYTIPQLPAISKLTGIVWLM
                     ASFAMVATAFLYITDNDTPHISKTID"
     gene            complement(567..>1630)
                     /locus_tag="PK348_10255"
     CDS             complement(567..>1630)
                     /locus_tag="PK348_10255"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HXK66640.1"
                     /translation="SPKDTRSAIAKFTDMAKAYLQRFTFQNDNEEEFILEDAVKAEII
                     DEPDAHDVTLKFIGEKEVNSKSNIPILYASLNAGIPHTHVCGGRARCSTCRVIIEEGL
                     EYCRPRNERETRLAKIKGFSPEVRLACQTTVEGNISLRRLVLDEHDISDAIRQGRGDI
                     NDVGREADVTILFSDIRSFTAFSEKALPYDVIHILNRYFETIGGIIDVNGGYIDKYMG
                     DGIMAIFGLDKEMKDDHAYLAVKSSLEIQHAIDGFNDYLSQHFNHKFKAGVGIHSGTV
                     IIGNLGFSKKKEYTALGDTVNTASRIESLNKRTGTAILVSESSYHRCQKNFTWGRKFK
                     TKVKGKEEPIVVYEVIDFM"
BASE COUNT          521 a          334 c          245 g          530 t
ORIGIN      
        1 aagtctattg ttttggagat atgaggtgta tcattatcag taatatacaa aaaagctgta
       61 gctaccatcg caaagcttgc catcagccaa actataccgg taagtttgct tattgcaggc
      121 aattgaggta ttgtataccc caaagatttt aaaaacccaa gaacatgaat aagaccatga
      181 agtataacaa taaaaaagat tatatatttc atatgttgcc tcctaaaaat acatattgat
      241 aagaactttc aaatacagct attaagggtg agattattga tatgttcacc ttttatctat
      301 gagcctacct ctaaaacagt attatctccc ttaattgaaa tttgcaatat ataaaaaata
      361 taaaaaaagt tattgatact atttatattg ctgcattaaa atgaaagaac cttataccat
      421 aaggaataca ttatgaaaac acaaaaacga atattggcaa tcaccggagc atttgggtat
      481 gttggttcgc gtgtcataga aaggcttaaa aagatttaaa ccacgttatt catcagagca
      541 ggcaattaga gcttatttat cttctactac ataaaatcaa ttacctcata aacaactatg
      601 ggctcttcct tgcctttaac ctttgtttta aattttcgtc cccaggtaaa atttttctgg
      661 caacgatgat atgaactttc agaaaccaat attgcagttc cagttctttt attcaatgat
      721 tctatacgtg aagctgtatt gactgtatca cccaatgcag tatattcttt ctttttgctg
      781 aaccctaagt tcccgatgat aaccgttcca gagtgaatac caacacctgc tttaaacttg
      841 tgattaaaat gttgtgatag gtaatcattg aatccatcaa ttgcatgttg tatttccagt
      901 gatgatttca cagccagata ggcatggtca tctttcattt ccttatccag accaaatatt
      961 gccattatac catcacccat atatttatca atgtagcctc cattaacatc aataatccca
     1021 cctattgttt caaaatacct gtttaaaata tgaatgacat catacggcaa agccttttca
     1081 gaaaatgcag taaaagaccg tatatctgaa aataatattg taacatctgc ttctcttcct
     1141 acatcattga tgtcacccct accctgccgt attgcatcgg atatatcatg ctcatccagt
     1201 acaagcctcc gcaatgagat gttaccttct acagtggtct gacatgcaag cctgacttca
     1261 ggggaaaacc cctttatttt agccagccgt gtttctctct catttcgggg tcgacaatat
     1321 tccagaccct cttctattat gaccctacag gttgaacacc gtgccctacc accacaaaca
     1381 tgtgtatgtg gtatccctgc attaagcgat gcatacagaa ttgggatgtt tgatttagaa
     1441 ttaacttctt tttcaccaat aaactttagt gttacatcat gtgcatcggg ttcatcaata
     1501 atctcagctt ttaccgcatc ttcaagaatg aactcttctt cattatcatt ctgaaatgta
     1561 aaccgctgta aataggcctt agccatatct gtaaatttag caattgctga ccttgtatct
     1621 ttagggctac
//
LOCUS       DAOSZP010000544         1629 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_55701_length_1629_cov_2.189327, whole genome shotgun sequence.
ACCESSION   DAOSZP010000544 DAOSZP010000000
VERSION     DAOSZP010000544.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1629)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1629)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1629
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55701_length_1629_cov_2.189327"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..610)
                     /locus_tag="PK348_10260"
     CDS             complement(<1..610)
                     /locus_tag="PK348_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013007885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide reductase N-terminal alpha
                     domain-containing protein"
                     /protein_id="HXK66641.1"
                     /translation="MSAKVQNWNEITIHSDKEPQLTENARIVLEKRYLAKDEEGNVIE
                     TPRQMFERIAKYIASADLLYGKDARHVNQVATTFYEMMVDLLFIPNSPTLMNAGRPLG
                     QLSACFVLPVEDSMDGIFDAIKYMALIHKSGGGTGFSFSRLRPKNDVVQTTKGVSSGP
                     VSFMNIFNAATETIKQGGTRRGANMAILHVTHPDIIEFIRAKE"
     gene            complement(830..>1629)
                     /gene="carA"
                     /locus_tag="PK348_10265"
     CDS             complement(830..>1629)
                     /gene="carA"
                     /locus_tag="PK348_10265"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HXK66642.1"
                     /translation="LDDYLKEYKVPGIEGVDTRKLTRHIRDKGAMRAGIFLDKAGALE
                     KVLYHPPMAGLDLTRNVMCQKPYTFGTHRDTAPTIAVFDFGVKTNILRLLTAEGFNVT
                     VYPGITPLSEVLKNTTIKGVFLSNGPGDPDAVDYAKVLVKDILKTEIPCFGICLGHQL
                     LALGLGAKTYKLKFGHRGGNQPVKNVLTQKVEITSQNHGFAVDYESTKKIPDIEITHI
                     NCNDTTVEGLRHKKLPLFCVQYHPEANPGPHDSRYLFKEFYSMVAQC"
BASE COUNT          490 a          355 c          284 g          500 t
ORIGIN      
        1 tttccttggc acggataaat tctatgatgt caggatgtgt aacatgcaat attgccatat
       61 ttgcaccacg tcgggtacca ccctgcttga tggtttctgt ggcagcatta aatatattca
      121 taaaggatac agggccactg gatacccctt tggtagtctg aactacatca tttttgggtc
      181 gcaacctgga aaaagaaaaa ccagtgcccc caccactttt atgaattaat gccatatact
      241 ttattgcatc aaatatacca tccatggaat cttctactgg taaaacaaaa cacgccgaaa
      301 gctgccccag tggcctgcct gcattcatta atgttgggct gtttggtata aaaagcaggt
      361 caaccatcat ttcataaaat gtggttgcta cctgattaac atgtcttgca tcttttccat
      421 acaacaaatc ggctgacgca atgtattttg caatgcgctc aaacatctgg cggggtgttt
      481 caataacatt gccttcctca tcttttgcca gataccgctt ttcaagaact atacgagcat
      541 tttcagtaag ctgaggctct ttatcagaat gtattgttat ttcattccag ttctgtactt
      601 ttgcactcac acaaattcct ccataaaata ataatacaca gttatatacc tgcaaataat
      661 gtatacaatt atacaaaaat aatcaatact actatgaaat actatgatca tgcgggaatc
      721 aaaaatacta cgtacgtatt aatatttgtc aacaaccatt tattaaaaag ttatactcaa
      781 ccatacgcta tcgttagctg aagaagttat attggcaatt agggtttaat taacactgtg
      841 ccaccattga ataaaattcc ttaaagagat atcgcgaatc gtgagggcct ggatttgctt
      901 caggatgata ttgtacacaa aataaaggca gttttttatg acgtaatcct tcaacagtgg
      961 tatcgttaca gttgatatgt gttatctcaa tatccggaat cttttttgtg gattcataat
     1021 ccactgcaaa cccatgattc tgcgaggtta tttcaacttt ttgtgttaat acatttttta
     1081 ccggctgatt cccacccctg tggccaaact tcaatttata cgtcttagcc cccaaaccaa
     1141 gcgccagcaa ctgatgccct aaacatatcc caaaacaggg tatctcagtc ttcagaatat
     1201 ctttcaccaa tacctttgca taatcaactg catcgggatc cccgggacca tttgacaaaa
     1261 acacaccttt aatagtagta ttttttaata cttccgataa cggtgtgatg cctggatata
     1321 cagtaacatt aaacccttca gcggtaagca gccgtaaaat gttggtttta acaccaaagt
     1381 caaatacagc aatagttggc gccgtgtcac ggtgtgtacc aaatgtatat ggtttctggc
     1441 acatgacatt gcgggttaaa tcaagtccag ccatgggcgg atgataaagc accttttcaa
     1501 gtgctccagc cttatccaga aaaatgcctg cacgcattgc ccctttgtca cgtatatggc
     1561 gggttaattt tcgggtatca acaccttcaa tgccgggaac tttatattct ttcagatagt
     1621 catcaagtg
//
LOCUS       DAOSZP010000545         1627 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_55779_length_1627_cov_4.055980, whole genome shotgun sequence.
ACCESSION   DAOSZP010000545 DAOSZP010000000
VERSION     DAOSZP010000545.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1627
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55779_length_1627_cov_4.055980"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1064)
                     /locus_tag="PK348_10270"
     CDS             complement(<1..1064)
                     /locus_tag="PK348_10270"
                     /inference="COORDINATES: protein motif:HMM:NF013318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HXK66643.1"
                     /translation="MNKEFVAHIGDDVILFNTGILAKQADGAVAVSCGETIVFASAVA
                     SKKVVEGQDFFPLTVDYREKHYSAGKIPGGFIKREGRPSDKEILTSRLADRPIRPLFP
                     DDFVNEVQVIIYVLSADREHQPDVMAINAASAALIISGIPFKGPVGAVRVGRINGQWK
                     INPYFQEMEHSDIDLVVAGTKKAVTMIEGQAKNLTEDEMIEAVEFAHANIIKLCEVQE
                     ALKEACGKPLMSYEPKLSDKELEKVIRERFFADIEQLKDVHEKKAREQAKDTLITTIT
                     SQLQEQFPDTIKLVSEIVDSLDAEVVRSRILNEGKRADGRGLKDIRPIDIMIGILPRA
                     HGSALFTRGQTQSLGIVTLGT"
     gene            complement(1107..1364)
                     /gene="rpsO"
                     /locus_tag="PK348_10275"
     CDS             complement(1107..1364)
                     /gene="rpsO"
                     /locus_tag="PK348_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006447003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HXK66644.1"
                     /translation="MAEKSEIINKYQLHDKDTGSAEVQIALLTERINHLTEHFKVHVK
                     DHHSRRGLLKLVGKRRRLLDYLKKKDLTKYRELIKSLGIRR"
BASE COUNT          420 a          404 c          264 g          539 t
ORIGIN      
        1 gttcccaggg ttactatccc caaactctgt gtctgtccac gagtaaacag tgctgaacca
       61 tgtgcgcgtg gcaaaatgcc aatcatgata tcaattggtc taatgtcttt gagtcccctg
      121 ccatctgcgc gcttgccctc atttaaaata cgcgagcgca ccacttcggc atctaagctg
      181 tctacaattt cagagaccag cttaatggta tcagggaatt gttcctgcaa ctgactggtt
      241 attgttgtaa tcagtgtgtc ttttgcctgc tctcgtgcct tcttttcatg aacatctttt
      301 aactgttcta tgtcagcaaa aaatcgttct cgtataactt tctcaagttc cttatcgctt
      361 aattttggtt cataggacat cagtggcttg ccacaggctt cttttaatgc ttcctgtact
      421 tcacacagtt ttatgatatt ggcatgggca aattcaacag cttctatcat ttcatcttca
      481 gttaaattct ttgcctgccc ttcgatcatg gtcacagctt tttttgttcc agccacaaca
      541 agatcaatat cactgtgttc catttcctga aaatatggat ttatcttcca ctgcccatta
      601 attctgccaa ccctcacagc cccaacaggc cccttaaaag gtatacctga tataatgagt
      661 gctgctgatg ctgcatttat tgccatcaca tccggctgat gctctctgtc agcagaaagc
      721 acataaataa taacctgcac ctcattgaca aaatcatccg ggaataaagg ccgtatgggc
      781 ctgtcagcca gcctgctggt taaaatttct ttgtcactgg gtcttccttc acgtttaata
      841 aacccaccgg gtatcttgcc cgctgagtaa tgtttttccc gataatcgac cgtcaacggg
      901 aaaaaatcct gtccttccac aaccttcttt gacgcaacag ccgaagcaaa tactatcgtt
      961 tcaccacacg aaactgccac ggcaccatcc gcctgctttg caagtattcc ggtattaaac
     1021 aggattacat catctccaat atgtgctaca aattccttat tcattgtata atccttaact
     1081 acttatttta aaatcttttg taccctttac cttcgtatgc caagtgattt aatcagttct
     1141 ctgtactttg ttaaatcctt cttcttaaga taatcaagaa gtcgtctgcg tttgcctaca
     1201 agcttcagca atccacgtcg tgagtgatgg tctttaacat gtactttgaa atgttcggtc
     1261 agatggttaa tcctctcagt taaaagggca atttgaacct ctgctgatcc ggtatccttg
     1321 tcatgcaact ggtatttgtt gataatctcg cttttttctg ccatacgact ttttagtcct
     1381 ccttacaatc actatattct aatttattat attcactgta agtcttataa ttcacagtgc
     1441 tatttgcagt caaaatcagt caagaaataa attataaaaa gcggggaaca cttttatttc
     1501 attgtatcaa ttttatcaag ctcttcaata aaaacttttt tacacctgta ccctttttca
     1561 tctttcacag ccagtgccac taagtcttca gtcccctcag gcactaccct gaatacaggc
     1621 tcaccgg
//
LOCUS       DAOSZP010000546         1613 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_56420_length_1613_cov_2.094352, whole genome shotgun sequence.
ACCESSION   DAOSZP010000546 DAOSZP010000000
VERSION     DAOSZP010000546.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1613
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56420_length_1613_cov_2.094352"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(31..>1613)
                     /locus_tag="PK348_10280"
     CDS             complement(31..>1613)
                     /locus_tag="PK348_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012528187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HXK66645.1"
                     /translation="YFIFIITTPVFFSLGSEFMPPLNEGTILYMPTTLPGISLTEARK
                     LLLYQDEVLKSFPEVESVFGKAGRAETSTDPAPLSMIETTVVLKPKEQWRKKEQWYSR
                     WAPEPLQQLLRHVWPDHISYSELIAMMDSTLKVPGVTNAWTMPIKGRIDMLTTGIRTP
                     IGIKILGGDIKEIEKIGMHIEGLIKDLEGTRSVYAERVGGGYYINIIPDRVALARYGL
                     TVEDLQTVVATAIGGQTVITTVENSRERYSINVRYQRDFRNNMDELKRVLIPTKNKFH
                     VPLQEVANITLSYGPSMIRNENGMTAGYVFVDIYDTVDIGTYVNNAKEVVYKSLQIPP
                     GYTVTFSGQYENILRVKQRLMIILPVTIILIVLILFINTGNIIKTIIVLLAVPFSLIG
                     AILLMYILGYNISIATWVGMIALMGLDAETGVFMLLFLDLSYEEARKYNRLKTIDNLK
                     NAIMHGAVQRIRPKMMTVMTTMIGLLPIMWSTGTGADMMKRIAAPMVGGLTTSFIMEL
                     LVYPAIYYLWKRRELNLH"
BASE COUNT          516 a          327 c          243 g          527 t
ORIGIN      
        1 attttttcat ataatcctcc tataaattta ttaatgtaaa ttaagttcac gccttttcca
       61 gagataatat attgcaggat atacaagcaa ctccataata aaggaagtgg ttaatcctcc
      121 aaccatagga gcagcaattc gtttcatcat atcagcacct gttcccgtag accacatgat
      181 aggtaatagc ccgatcatgg tagtcataac tgtcatcatt ttaggacgta ttctttgcac
      241 tgcaccatgc attattgcat ttttcaagtt atcaatcgtt ttcaatctat tatattttct
      301 ggcttcttca tatgaaagat ctaagaataa cagcatgaat actccagttt ctgcatcaag
      361 ccccatcaac gcaatcatgc caacccatgt agcaattgag atattatatc ccaggatata
      421 catcaacagt atggcaccaa ttaatgaaaa tggaacggcc agcaaaacta ttattgtctt
      481 aataatgttg ccagtattaa taaataaaat taatactatt aaaataattg taacaggcaa
      541 aataatcatt aaacgttgtt ttacccgcaa tatattttca tattgaccac tgaaggtgac
      601 agtataacct ggaggtattt gtaaggattt atagacaact tcttttgcat tatttacata
      661 tgttccaata tctaccgtat cataaatatc aacaaataca tatccagccg tcatcccatt
      721 ttcattcctg atcatcgatg gtccgtagga taatgtaata tttgccactt cctgcaacgg
      781 aacatggaat ttattttttg tagggattaa cacccttttc aattcgtcca tgttatttct
      841 gaaatctctt tgatatcgaa cattaattga atatcgctct ctagaatttt caacagtagt
      901 aataactgtt tgccctccaa tagccgttgc tacaacagtc tgcaaatctt caacagttaa
      961 accatatcga gctaatgcaa ctctatctgg aataatattg atataatatc ctcctcccac
     1021 acgttcagca tatacactgc gagttccctc caaatctttt atgagccctt caatgtgcat
     1081 accaattttt tcaatttctt taatatcccc acccagaatt ttaataccaa taggcgtacg
     1141 tataccggtt gttaacatat caatacgtcc tttaattggc atcgtccatg cattggtaac
     1201 tccaggaacc tttaaagtac tatccatcat tgcaatgagt tcagaatagg atatatgatc
     1261 aggccataca tgacgtaata attgttgtag tggttctgga gcccaccggg aataccattg
     1321 ttcttttttc cgccactgtt cttttggttt aagtactaca gtagtttcga tcatagataa
     1381 aggagccgga tcagttgaag tttctgctct acctgctttg ccaaatactg attcaacctc
     1441 aggaaagctt tttaacactt catcctgata caacaaaagt tttctggctt cagtaagtga
     1501 tatacctggc aatgtggtag gcatataaag aatagtacct tcatttaaag gtggcataaa
     1561 ttctgaaccc agtgaaaaaa agactggtgt tgtgataata aaaataaaat aga
//
LOCUS       DAOSZP010000547         1611 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_56477_length_1611_cov_3.978792, whole genome shotgun sequence.
ACCESSION   DAOSZP010000547 DAOSZP010000000
VERSION     DAOSZP010000547.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56477_length_1611_cov_3.978792"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1328)
                     /gene="argS"
                     /locus_tag="PK348_10285"
     CDS             complement(<1..1328)
                     /gene="argS"
                     /locus_tag="PK348_10285"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HXK66646.1"
                     /translation="MIIKEYIAQTVGQALTKLLQEKNISTELSPAIKVEYPKEEKFGD
                     YATPVAMECAKILRMSPIQIGEEIKQHINADVFDRIDVVKPGFINIFLSLPFLLQRLE
                     EIVTVKDEYGKNKKSNPRKVNIEFVSANPTGPLNVVSARAAAVGDTLANLLAASGDTV
                     HREFYVNDYGNQVLWLGKSVWIRYRQMLGEDVPFPEEGYHGEYVKDIAKYIHENFSQE
                     LSHINNEEEIINFCAQKAIEYNVASQKEDLDKFNVSFDTWFHESTLHHSGKVLDVFKY
                     LESLGVIRDEDGKKVFVSTQFGDDKDRVVVRDDGRPTYLMADIAYHRTKIERGYDLII
                     DIWGPDHHGYIARLVGAMKAMGYDEKRFRILISQQVNLLMNGEAVKMSKRLGTFSTMR
                     DLIEEIGTDVARYFFVMRSMDSHLDFDIDLAKRQSSENPVFYLQYAHARI"
BASE COUNT          501 a          339 c          239 g          532 t
ORIGIN      
        1 cagattcggg catgagcata ctgcaaataa aatacaggat tttcactgct ctggcgtttt
       61 gccaagtcaa tatcaaaatc taaatggctg tccattgaac gcatgacaaa gaaatatcgc
      121 gcaacatcag tcccaatctc ttcaatgaga tcacgcatgg tggaaaatgt ccccaaccgt
      181 ttagacattt tcaccgcttc accattcata agaaggttta cctgctggga aatcagaatc
      241 ctgaatcgtt tttcatcata acccattgct ttcattgcac caaccaggcg ggcaatatac
      301 ccatgatgat caggccccca tatatcaatg attaaatcat acccacgttc aatctttgtc
      361 ctgtggtatg caatatcagc cattaaatat gttggcctac catcatctcg tacaaccacc
      421 ctatccttgt catcaccaaa ctgtgtggaa acaaaaacct ttttgccatc ttcatctcgt
      481 ataacgccaa gtgattctaa atatttaaaa acatccaata ctttgccact gtgatgcagg
      541 gtgctttcat gaaaccatgt atcaaaacta acattaaatt tatccagatc ctctttttgc
      601 gatgcaacat tatattcaat agctttttgt gcacaaaaat tgatgatctc ttcctcattg
      661 tttatgtgcg atagttcctg tgaaaagttt tcgtgtatgt atttagcaat atcctttaca
      721 tattcaccat gatagccttc ttcaggaaat gggacatctt ctcctaacat ttgcctgtag
      781 cgtatccaca ctgatttccc taaccacaaa acctgattgc cataatcatt gacataaaat
      841 tcacggtgaa cagtatcacc acttgcagca agcaaatttg caagggtatc tcccactgcg
      901 gctgcacgtg cagaaaccac atttaatgga ccagtaggat ttgccgacac aaactcaatg
      961 ttgaccttgc ggggattgga ttttttattt ttaccatatt catctttgac agtaactatc
     1021 tcctctaatc tttgtaaaag aaatggcagt gatagaaata tattgataaa gccaggctta
     1081 accacatcta tacgatcaaa aacatcagca ttaatatgct gtttaatttc ttcgccaatc
     1141 tgaattgggg acatccttag aatttttgca cactccatgg caacgggagt ggcataatca
     1201 ccaaattttt cctctttggg atactcaact tttattgctg gcgatagctc tgttgaaata
     1261 tttttttcct gcaatagttt tgttaaagcc tgaccgacag tttgtgcaat atactccttg
     1321 attatcaaaa caacacctct ttacataatt cccagtttta taatatgcag tatatcacga
     1381 aaaattaatt gaaaagatac aaattgattt tttgcattaa ttattttgtc aagaatttaa
     1441 taaaaaactt gcaattatca cataattatt gtattgcatt ttacagattc ctttaatcaa
     1501 aaatcaaact attatcttat aaatcataag ggtatccact atgcaaatta ataagaatca
     1561 atttctgggt ttggttttat cagtagttgt atttattatt ctttattttt t
//
LOCUS       DAOSZP010000548         1609 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_56573_length_1609_cov_6.138353, whole genome shotgun sequence.
ACCESSION   DAOSZP010000548 DAOSZP010000000
VERSION     DAOSZP010000548.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1609
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56573_length_1609_cov_6.138353"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            29..1060
                     /locus_tag="PK348_10290"
     CDS             29..1060
                     /locus_tag="PK348_10290"
                     /inference="COORDINATES: protein motif:HMM:NF024183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HXK66647.1"
                     /translation="MSNQNSPLKGFRPLKDSKTPSENPPNTTWKSSNPAPYQPPQQFS
                     SSPNINPPQQKTICPYCSSPIPAGSSICLNCGNPVTQPNATSAAPTGNFCPHCGASNP
                     TNASFCNKCGTSLIRPNPSPTYTPPTLNLPSNIQNVVTTLKTDTERFWTYVGAATAIL
                     AFICFFLPFLVMKINNAFSFLIGGPDSITLSLSGYQYLTAQSPSMSGVGSLGDASENL
                     FNELDLGNQMYQYADPSVRRTYIISRIMTLIILLLSIANLVVIYQAYATKKAELSKWM
                     LALGIVGFILLFINYFLAGISFKTGTEELDLLVNAMVKISNGIGFWGMLIGFLGVAFS
                     GYMRNKKTI"
     gene            1078..>1609
                     /locus_tag="PK348_10295"
     CDS             1078..>1609
                     /locus_tag="PK348_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000966847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HXK66648.1"
                     /translation="MAYNVLATSQTPALIIYLLDVSGSMSTNLNGKPRIEVVTDALSA
                     TLQQMVFRCTKGGQISPRYRIAMYAYSDIVYDILGGVMTIDQVANLGVPELSTMRSTA
                     TDLAFEEVERLLQQELPSLNDCPAPLVCHMTDGEYTGEDPEPIVRRIMRMSNADGNVL
                     VENIFISDQLLDEPVTD"
BASE COUNT          453 a          382 c          311 g          463 t
ORIGIN      
        1 gttgttgctt gtatcttagg aggaaagtat gtctaaccaa aatagtcccc tcaaaggttt
       61 tcgtccactc aaagacagta aaactccatc agagaatcca cctaatacaa catggaaaag
      121 ttccaatccc gccccctatc aacctcctca acaattttca agttctccca atatcaatcc
      181 cccgcaacaa aagacaatat gcccatattg ttcaagcccg attccagccg gctcctcgat
      241 ttgtttaaat tgtggtaacc cggtgacaca accaaacgct acctcagctg caccgactgg
      301 aaatttttgt cctcattgtg gggcatcaaa cccaacaaac gcttcattct gtaataaatg
      361 tgggacatct ctaatacgcc ccaatccttc acctacttat acacctccaa cattgaattt
      421 acccagcaac attcaaaatg tagttacaac tctgaaaaca gataccgagc gtttttggac
      481 atatgttggc gctgctacag caattctagc gtttatttgc ttcttcttac cattcttagt
      541 tatgaaaatc aacaatgcat tctcatttct tatcgggggt cctgattcta ttactttatc
      601 attaagtggt taccaatatt taaccgctca atccccatcg atgtcaggtg tgggaagttt
      661 aggggatgcg tccgaaaacc tatttaatga gctcgacctg ggtaaccaaa tgtatcaata
      721 tgcagatcca tctgtacgta ggacatatat catcagtcgt ataatgacat taattatcct
      781 actattgtct attgcaaatt tggttgtgat atatcaggct tacgccacaa aaaaagccga
      841 attaagtaaa tggatgcttg ccctaggcat tgtaggtttt atcctcctat ttattaatta
      901 tttcttagct ggtataagct tcaaaactgg aaccgaagaa cttgacttat tagtcaatgc
      961 aatggtcaag atttccaatg gtattggctt ttggggaatg ctgattggat ttttgggcgt
     1021 tgctttttct ggctacatgc gcaacaagaa aacaatttaa attattggag gatttctatg
     1081 gcatacaatg tgttagcaac gagtcaaacc cctgccctca ttatttactt gctggatgtc
     1141 agcggatcga tgtctacgaa cttgaacggc aaaccacgca tcgaagtggt tacagatgca
     1201 ctctcagcaa ctttgcagca aatggtgttc cgctgtacta agggcggtca aatttctccg
     1261 cgttaccgca tcgcaatgta cgcttatagc gatatcgttt acgacatcct tggcggagtg
     1321 atgaccatcg atcaggttgc caacctgggc gtccccgagt tatcgacgat gcgctccacc
     1381 gccaccgacc ttgcatttga ggaagtcgaa cgcttattgc agcaagaact gccaagctta
     1441 aatgactgcc ctgctccttt ggtttgccat atgaccgatg gggaatacac tggcgaggat
     1501 ccagaaccta ttgtgagacg cataatgcgc atgtctaacg cagatggcaa cgtactcgta
     1561 gaaaatattt tcatatctga ccagctgctc gacgagcccg taactgata
//
LOCUS       DAOSZP010000549         1608 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_56628_length_1608_cov_3.181584, whole genome shotgun sequence.
ACCESSION   DAOSZP010000549 DAOSZP010000000
VERSION     DAOSZP010000549.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56628_length_1608_cov_3.181584"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1608
                     /locus_tag="PK348_10300"
     CDS             <1..>1608
                     /locus_tag="PK348_10300"
                     /inference="COORDINATES: protein motif:HMM:NF014788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HXK66649.1"
                     /translation="SSVHKKAMPGDNIVMVGGRVGRDGIHGATFSSEALTSGSPATAV
                     QIGDPITQKKFSDAIVKEARDLGLYHSITDNGAGGLSCSVSEMAREAGGFIVYLEKVP
                     LKYPGLAPWQIWVSESQERMTLSVPDETLEDFLNLMKRRGVEAWVIGKFTDSGRGIVY
                     YNGEIVFNIDLHFLHEGLPQKTLTSTYTPKSHPYPEFDDPENITEHFIAMVKRLNTAS
                     FEFISTQYDHEVQGNSVIKPLQGKGKVNGNATVIRPLLESTKGVVLSQGLYPSYSDID
                     TYWMAACSIDTAIRNAVATGCDLDKLALLDNFCWCDSTNPYRLGQLKEAARACYDFAV
                     AYKTPFISGKDSMFNDFKGYDEHFNEVFISVPPTLLISSIGVVSDISYCQTLDFKNAG
                     DYVYILGITRDELGGSEYLAYMGETLSGERYIGNTIPHVDTIMNTKVYRVIEKALRAG
                     LIASSISIERGGIALAIAKSAMGGLLGANIDLRVIPHENINRNDTLLFSESQGRILVS
                     IAPENKTQFEKLCKDIPFAKIGQVTDN"
BASE COUNT          493 a          276 c          358 g          481 t
ORIGIN      
        1 aatcttcagt acataaaaaa gcaatgcctg gagataacat agttatggtt ggcggcagag
       61 tggggcgcga tggcattcat ggagcaacat tttcttcgga agctttaaca tcaggcagtc
      121 ctgcaacagc ggttcagatt ggtgacccca ttacacagaa aaaattttct gatgcaatag
      181 tgaaagaagc acgagattta gggttgtatc actcaataac cgataacgga gctggtggac
      241 tttcatgttc ggtttccgag atggcacgtg aagctggcgg ttttattgta tatttagaaa
      301 aggttccgct gaaatatcct ggccttgcgc catggcagat atgggtatca gaatcgcagg
      361 aacgtatgac cttatcagtt cccgatgaaa cgcttgaaga ttttcttaac ctgatgaaac
      421 gccgtggtgt tgaagcatgg gtaattggca aatttacaga ttccggaaga ggaattgtgt
      481 attataatgg ggagatagtt tttaacattg accttcattt tttgcatgaa ggcctccctc
      541 agaaaacgct cacaagcacc tatactccaa agtcccatcc atatcctgag tttgatgatc
      601 cggaaaatat aaccgaacat tttattgcaa tggttaaacg cttaaatact gcaagctttg
      661 aatttatttc aactcagtat gaccatgaag tgcagggcaa cagtgtaatt aaacctctgc
      721 aaggtaaagg gaaggtcaac ggaaacgcta ctgttatacg acctttgttg gaatcaacaa
      781 agggagtggt tttgtcacag ggattatatc cttcatacag tgatattgat acgtactgga
      841 tggcagcctg ttcaatagat actgcaattc gcaatgctgt tgctaccgga tgtgatctgg
      901 ataaacttgc attgcttgat aatttctgct ggtgtgattc tacaaatcca tacaggttag
      961 gccaattaaa agaagctgca cgtgcatgtt atgattttgc tgttgcgtat aaaacaccat
     1021 ttatcagcgg caaagacagc atgtttaatg atttcaaagg ttatgatgaa cattttaatg
     1081 aggtatttat atcagtaccg ccaacattgt taatttcttc tattggagtt gtttctgata
     1141 tttcttattg tcaaactctt gattttaaaa atgctggcga ttatgtatat atacttggca
     1201 ttaccagaga tgaattgggt gggagtgaat atttagcgta tatgggtgaa acgctttctg
     1261 gggaaagata tattggcaat actatcccgc atgttgatac catcatgaat actaaagtat
     1321 atagagtaat agaaaaagca ttacgtgcag gcttgattgc ttcaagtata agcattgagc
     1381 ggggtggcat agctctggct atagcaaaat cggcaatggg cggattactg ggagcaaata
     1441 ttgatttacg tgttattcca catgaaaata ttaatcgcaa cgacacacta cttttttcag
     1501 aatcacaggg aagaattctt gtgtcgatag ctcctgaaaa taaaactcaa tttgaaaaat
     1561 tatgtaagga catacctttt gctaagatag gacaggtcac agataatt
//
LOCUS       DAOSZP010000550         1603 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_56854_length_1603_cov_3.501938, whole genome shotgun sequence.
ACCESSION   DAOSZP010000550 DAOSZP010000000
VERSION     DAOSZP010000550.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56854_length_1603_cov_3.501938"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..601)
                     /locus_tag="PK348_10305"
     CDS             complement(<1..601)
                     /locus_tag="PK348_10305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66650.1"
                     /translation="MFINFFFNLKAQGVPVSIHEWIALHEALALNLNDTSLTKFYYMA
                     RAILVKNEVFYDRYDIAFLDTFKDVETTDELLEKILEGLKKVKELHLTEEEKRQIEKL
                     NLDEILRNFEEQLRQGHYKNHVGGNKAIGTGGRSTQGAWGYNPAGIRIGQGVSRHKSA
                     VQIAEKREFKNYSNQVVLDTRQMRVALSHLRSLLPIGPEE"
     gene            complement(591..1448)
                     /locus_tag="PK348_10310"
     CDS             complement(591..1448)
                     /locus_tag="PK348_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HXK66651.1"
                     /translation="MMNYFTGTEDYVISQELKDNVNVSIALGRPLLVKGEPGTGKTLL
                     AHSIAKGLGKRLLVWNIKSTTKAQEGLYVYDTVQRLNDARFGDKDVSNIKQYIKLGKL
                     GEAFASEDQVVLLIDEIDKADIEFPNDLLHELDIMNFYIPETGETITAKHRPIVIITS
                     NSEKELPDPFLRRCVFHYIAFPDEELMEKIVRVHFPNIEKKLLRECLKKFYWIRQFDM
                     LRKKPSTSELVDWIQALMVGGVPVKKIEDELPFIGTLLKKKEDIDVVVNSGKTHYAYN
                     SSIRTRDVY"
BASE COUNT          471 a          367 c          251 g          514 t
ORIGIN      
        1 tttcttcagg gccaataggc aaaagcgaac gcagatgtga taatgccacc cgcatctgtc
       61 gagtgtcaag aaccacctgg ttagagtaat tcttaaactc acgcttttca gctatctgta
      121 ctgcactttt gtgacgtgaa acaccctgtc caatcctgat gcctgccgga ttataccccc
      181 aggcaccctg cgtggaacgt ccaccagtac caatagcttt attaccacca acatgattct
      241 tgtagtgccc ctggcgcagt tgctcttcaa agttccgcaa tatttcatct aaattcagct
      301 tttcaatctg acgcttttcc tcttcggtga ggtgtaactc ttttactttt tttaagcctt
      361 ccaaaatctt ttcaagcagt tcatccgtag tttctacatc cttgaatgta tcaaggaatg
      421 caatatcgta tctatcgtaa aacacctcat tttttacaag gatagcccgt gccatataat
      481 aaaatttagt taagcttgta tcattcagat ttaaagccaa cgcttcatgc agtgcaatcc
      541 actcatgtat tgatacgggc acaccctgtg ctttaagatt gaagaaaaaa ttaataaaca
      601 tcgcgtgtcc ttatactgct attatatgca taatgtgttt tccctgaatt aacaactaca
      661 tcaatgtctt ctttcttctt caaaagagta ccaatgaaag gcaattcatc ttctatcttt
      721 ttgacaggta ctccaccaac cataagcgcc tgaatccagt caacaagctc gctggttgag
      781 ggttttttcc gcaacatatc aaactgccgt atccagtaaa atttcttcaa acattcacgt
      841 aaaagttttt tttcaatatt ggggaaatgc acccgcacaa tcttttccat aagttcctca
      901 tcaggaaatg ctatataatg aaatacacat cttcgtaaaa atggatcagg caactctttc
      961 tcgctgttgg atgtaataat tacaataggg cggtgttttg ctgttattgt ttcgcctgtt
     1021 tcaggtatat aaaaattcat aatgtcaagc tcatgtaaca ggtcattggg aaactcaata
     1081 tcagccttgt caatttcatc aattaacagt accacctggt cttctgatgc aaacgcttcg
     1141 cctaacttcc ctaatttaat atactgttta atattagaaa catccttatc gccaaatcga
     1201 gcatcattca aacgctgcac tgtatcatac acataaagcc cttcctgagc ctttgttgtt
     1261 gatttaatat tccatactaa aagcctttta cctagtcctt ttgcaataga atgtgcaagc
     1321 aacgttttgc ctgtgcccgg ttcacctttt accagcaacg gccgtcccag agctatcgac
     1381 acatttacat tatctttaag ttcctgggaa ataacataat cttcggttcc ggtaaaataa
     1441 ttcatcatgc acctcaatat actttttttc acgatagttt attagaaagc tgcagcatgc
     1501 atagcatatg gctataaaaa gcagaatgcc atttactttc tatagttaca gtgtagtgca
     1561 aaatcagtta aaaacaaaaa attacactgt tatgttccaa tac
//
LOCUS       DAOSZP010000551         1600 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_57006_length_1600_cov_2.223948, whole genome shotgun sequence.
ACCESSION   DAOSZP010000551 DAOSZP010000000
VERSION     DAOSZP010000551.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1600
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57006_length_1600_cov_2.223948"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..561
                     /locus_tag="PK348_10315"
     CDS             <1..561
                     /locus_tag="PK348_10315"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HXK66652.1"
                     /translation="VIHRFHPEISFEEARRRYLETSGVPFFQQLEIILPGNPTNSQKA
                     AIFEETKKEGFFRSTFSQEVRYVINELRRLGFIVGVSSNNFQYLIEQFINREGLQFDV
                     VLGFRDGFEKGKQHFEYVIQHFNLQKDDLTFVGDSLKDAEKAITNNIKFIGLCGTFTR
                     EQFLQKYPNIVTIESLKELLSCVQLY"
     gene            543..1274
                     /locus_tag="PK348_10320"
     CDS             543..1274
                     /locus_tag="PK348_10320"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HXK66653.1"
                     /translation="MRAIVLAAGTGSRLGEMTKNKTKGMVQVNGKPLIDYLFEFFDLN
                     FFDEIIVVGGFAFNDLEKHLKQKQIEHLKILENKEYHKGNIFTLITALKTFAHDSFLI
                     TNVDHIYPKVMFETMKQSFTRITAMCDFDRQLGPDDMKVKLDVDRKTIIHISKQLHDF
                     DCGYIGMTYVDRTMETVYRDSAYRAIERFGEKAVVENILQILAENPRTAPTICDLSGF
                     GWYEVDDEQDLIKAEKGLFQNPNFS"
     gene            complement(1280..>1600)
                     /locus_tag="PK348_10325"
     CDS             complement(1280..>1600)
                     /locus_tag="PK348_10325"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HXK66654.1"
                     /translation="LEHGMAFHNGLDICAPQGTPIKPVQSGFVNFTGWLDGYGFTIII
                     QHDEGYESWYGHCQKIFAQTGEFVTTDTMIGTVGSTGRSTGPHLHFMLLQHGKMLNPI
                     MFIW"
BASE COUNT          549 a          245 c          318 g          488 t
ORIGIN      
        1 gttatacata gatttcatcc ggaaatttcg tttgaggaag cacgtagaag atacttggaa
       61 acatcgggtg ttcctttttt tcagcaactg gagataatct taccgggaaa tccaaccaat
      121 agccagaaag ctgcaatctt tgaagaaaca aaaaaagaag gctttttcag atcaacgttt
      181 tcgcaagagg tgcgctatgt gatcaatgaa ttacgacgcc tgggatttat agttggagtt
      241 tcatcaaata attttcaata tctcatagag caatttatca atagagaagg gttgcaattt
      301 gatgtagtac tgggttttag agatggtttt gaaaagggca agcagcattt tgaatatgtt
      361 attcagcatt ttaatttgca aaaagatgat ttgacttttg ttggcgattc actaaaagat
      421 gcagaaaagg ctataacaaa taatataaaa tttataggtt tgtgtgggac atttaccaga
      481 gaacagttct tgcaaaaata tccaaatatt gtaacaattg aatcattgaa ggagttatta
      541 tcatgcgtgc aattgtatta gcagcaggta ccggtagtcg cttgggtgaa atgactaaaa
      601 acaaaaccaa agggatggtt caggttaatg gaaagcctct tatagactat ttatttgaat
      661 tttttgatct taattttttt gatgaaatca ttgttgtagg tggttttgcc tttaatgatc
      721 ttgaaaagca tttgaaacaa aaacagatag aacatttaaa aatacttgaa aataaggaat
      781 accacaaagg caatatattt acattaatta ccgcattaaa aacatttgcc catgattcat
      841 tccttataac caatgttgat catatttatc caaaggttat gtttgaaaca atgaagcaat
      901 cttttactcg aattactgct atgtgtgatt ttgacagaca actgggtcct gatgatatga
      961 aggttaagtt agatgttgat aggaagacaa taattcatat aagcaaacaa ttgcatgatt
     1021 ttgattgcgg gtatataggg atgacgtatg ttgatagaac catggaaaca gtttacagag
     1081 attcagcata ccgtgcaata gaaaggtttg gtgaaaaagc ggtagttgaa aatattttac
     1141 aaatactggc agaaaatcca aggactgcac caacaatatg tgacctttca ggatttgggt
     1201 ggtatgaagt ggatgatgag caagatctta taaaggcaga aaaagggtta tttcaaaatc
     1261 caaatttttc ttaaaaaagc taccagataa acatgatagg attaagcatc ttgccgtgtt
     1321 gcagtagcat gaaatgaaga tgaggacctg ttgatcttcc tgttgaacct acagttccaa
     1381 tcatagtatc agttgtcaca aactcacctg tctgggcaaa tattttctgg caatgtccat
     1441 accagctttc atagccttca tcatgctgaa taatgattgt aaacccatac ccatccaacc
     1501 aaccagtaaa attgacaaat ccactttgga ctggttttat tggagtaccc tgtggtgcac
     1561 agatatcaag cccattatgg aaagccatac cgtgctcaag
//
LOCUS       DAOSZP010000552         1596 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_57147_length_1596_cov_3.464633, whole genome shotgun sequence.
ACCESSION   DAOSZP010000552 DAOSZP010000000
VERSION     DAOSZP010000552.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1596
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57147_length_1596_cov_3.464633"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            5..1003
                     /locus_tag="PK348_10330"
     CDS             5..1003
                     /locus_tag="PK348_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007603357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HXK66655.1"
                     /translation="MVFLIALALVFDFLNGFHDAANSIATIVSTRVLSPRYAVIWAAF
                     FNFIAFLVFGLHVAGTVGSGIIDINIIDNTIILATLTGACLWNIITWYLGLPTSSSHA
                     LIGGLIGAALVKVGVNALMFDGIMKTVIFIFVSPIVGFILGLMFGIAIHWIFQHAHPA
                     RVDKLFRKGQLISAAFYSLGHGGNDAQKTMGIIAGLLFSNGLMHIAHGQRFQDAIPLW
                     VVISCQTAIALGTMFGGWRIVKTMGQRVVKLRPVDGFCAEFGAAFNLFVITSYFGIPV
                     STTHTITGAIMGVGSLKRFTAVRWGVAGQIVWAWVLTIPLAAIISGLTYFVAAHWL"
     gene            complement(1015..>1596)
                     /locus_tag="PK348_10335"
     CDS             complement(1015..>1596)
                     /locus_tag="PK348_10335"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HXK66656.1"
                     /translation="ILIKGPMVFKGYNKMTPEEKAEIFTPDGYFRSGDIGIIDEDGYL
                     RITGRLKDIIITSQGKNIVPLNIEKKFESEPLINYICVVGDRRKYLSALIVPNFHLLR
                     QYAREHGISYKTDEELVSHPQVQTLYKTRINEICQALPPYEQIKKFTLISHDFSVKSG
                     ELTPTFKFRRSQIHEKYKDIIDLMYPKSDITRL"
BASE COUNT          417 a          262 c          349 g          568 t
ORIGIN      
        1 gtatatagta tttttgattg cacttgcgtt ggtatttgat ttcctgaatg ggtttcatga
       61 tgcggccaat tcaatagcaa caatcgtttc tacaagggta ctatcgcccc gatatgctgt
      121 tatatgggca gcatttttta atttcattgc ttttcttgtt tttggattgc atgtagcagg
      181 tacggttggg agtggcatta tcgatatcaa tatcattgat aatacaatta tactggctac
      241 gttaaccggt gcctgcctgt ggaatatcat tacatggtat ttaggtttgc ctaccagttc
      301 gtcacatgca ctcattggag ggttaatagg tgctgcgctt gtgaaagttg gcgtaaacgc
      361 attaatgttt gatgggataa tgaaaactgt tatttttatt tttgtttcgc cgatagttgg
      421 ctttatatta ggtcttatgt ttggaattgc cattcactgg atttttcagc atgctcatcc
      481 tgcgcgtgtt gataagttat tcaggaaggg tcagttaata tcagcagcgt tttacagttt
      541 aggacatggt ggcaatgatg cgcagaaaac gatgggtata attgcaggat tgttgttcag
      601 caatggttta atgcatattg cacatggtca aagatttcag gatgcaatac cattatgggt
      661 agtcatttca tgccaaactg caattgcatt gggtaccatg tttgggggat ggcgtattgt
      721 gaagacgatg ggacaacgtg tagttaaatt aagacctgtt gatgggtttt gtgctgaatt
      781 tggcgctgca tttaatctgt ttgttataac ttcatacttt ggcataccag tcagtacaac
      841 tcataccata acgggggcta taatgggtgt ggggtcacta aagcgattta cagctgtgcg
      901 atggggtgtt gccggccaga tagtgtgggc atgggtactg actataccac tggcagcgat
      961 aatatcagga ttaacgtatt ttgttgctgc acattggttg taatattatt cctgttacaa
     1021 tcgtgtgata tcactctttg gatacatcag gtctataata tctttatatt tttcatgaat
     1081 ctggcttcgc ctgaatttaa acgttggagt cagttctccg ctttttaccg aaaaatcatg
     1141 cgaaatcagt gtgaattttt ttatttgttc atatggtggt aatgcctgac agatttcatt
     1201 aatccttgtt ttatataatg tttgaacctg tggatgggat accagttctt cgtcggtttt
     1261 gtatgaaatt ccatgttctc gggcatattg ccgcaaaaga tgaaaattag gaacaatgag
     1321 tgctgataag tattttcgtc tgtcaccaac cacacagata taatttatca aaggttctga
     1381 ttcaaatttc ttttcaatat tcaatggtac aatatttttg ccctgtgaag taataatgat
     1441 atctttaagc ctaccggtta tacgtaaata gccatcttca tctataatac caatgtcgcc
     1501 tgatctgaaa tagccatcag gtgtaaaaat ttctgctttt tcttctggag tcattttatt
     1561 gtaaccctta aaaaccatgg gacctttaat aagaat
//
LOCUS       DAOSZP010000553         1591 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_57390_length_1591_cov_3.337891, whole genome shotgun sequence.
ACCESSION   DAOSZP010000553 DAOSZP010000000
VERSION     DAOSZP010000553.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1591
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57390_length_1591_cov_3.337891"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            124..840
                     /locus_tag="PK348_10340"
     CDS             124..840
                     /locus_tag="PK348_10340"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HXK66657.1"
                     /translation="MEERKVVYDDEIDLYELFIKLWKHKIFILMFTLLCTVASIVAVN
                     IMPPKYKVTAKVVPNNIEFIKIFYKDLGSYGNIDMVLSNIYIGVYTEIIFPLFKSRDL
                     AIKAVGQALGGNSDPEYCASIIDEEELSIDVKKNREVASVIEIEVVHRDSALAYNFAN
                     NLLLQVDNELKRIYNIKDSKTRLLNVTESPVMPKKPYSPKKSLVVAVAFVSSLFLALF
                     LVLIIDWIGAVRKQYSPDRM"
     gene            917..1453
                     /locus_tag="PK348_10345"
     CDS             917..1453
                     /locus_tag="PK348_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HXK66658.1"
                     /translation="MLDALITSKTRLKILLKFFINPAIKGYLRELAAQMNESTNAVRV
                     ELDRLVAAGYLERSASGNKIFYSANTKHPLYRDIHNVVKKYVGIDVLIDDVLSKLGEV
                     NEAYVVGDYAKGIDSGIIDVVIIGQVDVLYLAELIQKAEGIIKRKIRSLVLSPREKKK
                     YGGKIDLPSGLLVWSNEK"
     gene            1443..>1591
                     /locus_tag="PK348_10350"
     CDS             1443..>1591
                     /locus_tag="PK348_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012474397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HXK66659.1"
                     /translation="MRSSWYAVHVRSRHEFKVRESLEAKGIETFLPTVERLRQWKDRK
                     KKVLFP"
BASE COUNT          490 a          220 c          381 g          500 t
ORIGIN      
        1 aaagaaaaag agagctttgg tggagtagct taaggcattt acaaaaatta ataccgtgaa
       61 taaagcagag ttgttttacc tgtgctttgc ttaaattata aatataattg aggagctttg
      121 tcgatggaag aaagaaaagt agtctatgat gatgaaattg atctctatga gctatttata
      181 aaattatgga agcataaaat atttatattg atgttcacac ttttgtgtac tgttgcatca
      241 attgtggcag ttaatattat gcctccaaaa tataaagtaa ctgcaaaggt agtcccaaac
      301 aatattgaat ttattaaaat tttttataag gacctgggta gctatggcaa tatagatatg
      361 gtgttgagta atatatatat tggtgtctac actgaaataa tttttcctct ttttaaaagt
      421 agagatctgg caattaaagc ggttggccag gctcttggtg gtaattcgga ccctgaatat
      481 tgtgcaagta ttatagatga agaagaattg tcaatagatg ttaaaaaaaa tagggaagtt
      541 gcaagtgtta tagaaattga ggtagttcac agggattctg cccttgccta taactttgcc
      601 aataatttat tgcttcaggt ggataatgag ctaaaaagaa tatataacat aaaggattcc
      661 aaaacccgtc ttctgaatgt cactgaaagc cctgtaatgc caaaaaaacc ttatagtcca
      721 aagaaaagtt tagttgttgc tgtggccttt gtgtcatcac tatttttggc tttgtttttg
      781 gtgcttatta ttgattggat aggagcagtg cgtaagcagt attctcctga tagaatgtag
      841 tttaggccct ttgtttagag ggtagaaatt gaaaattcct tgacaaaaat gagtaattat
      901 tttactcatt tttaatatgc ttgatgcgct tattacatcg aaaacacgac ttaagatttt
      961 gctgaaattt tttataaacc cggcaattaa gggttatctc cgtgaactgg cagcgcagat
     1021 gaatgaatcg acaaatgctg tgcgtgtgga gcttgatcgt cttgttgcgg caggctacct
     1081 tgagcgaagc gccagtggta ataaaatttt ttacagcgca aatacaaaac atccccttta
     1141 tcgggatatt cacaatgtgg tgaaaaagta tgtgggtatc gatgtattga ttgatgatgt
     1201 gctttcaaaa ctcggtgagg ttaatgaggc ttatgtggtg ggggattatg ccaaggggat
     1261 tgacagtggt ataattgatg tggtgattat agggcaggtg gatgttttgt atctggctga
     1321 gcttattcaa aaggcagagg gtataattaa acgcaaaata cgttctctgg tgctgtctcc
     1381 cagggaaaag aaaaagtacg gtgggaagat agatttgcct tcagggcttc tggtgtggag
     1441 caatgagaag tagctggtat gcagtgcatg tgcgttctcg ccatgagttt aaggtgaggg
     1501 agagtcttga ggcaaagggg atagagacct ttttgcccac agtggagcgg ctgcgtcagt
     1561 ggaaagaccg caaaaagaag gttctgtttc c
//
LOCUS       DAOSZP010000554         1581 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_57880_length_1581_cov_2.610092, whole genome shotgun sequence.
ACCESSION   DAOSZP010000554 DAOSZP010000000
VERSION     DAOSZP010000554.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1581
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57880_length_1581_cov_2.610092"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..991
                     /locus_tag="PK348_10355"
     CDS             <1..991
                     /locus_tag="PK348_10355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66660.1"
                     /translation="KDIYTDIHEQGPSLNSHLIHKEPNAPYRLFDFISNDGTLKEGRI
                     SYINSIKPHLVVSIHFALNSSPYFRGMNAVIAAPYSILYKGLQYFQGTLTDRSFFYHS
                     KYADWFTEDERKSGFFWYSNDVVMYFTGYRIKNDYSINTDAFRGYRYNMVQWAFSDPP
                     GWAHIAKHHPPKTPYANNIQQFIPQNAFFTREQSKYEQYRRDGGFEGYGGDNLYASNE
                     IIRFVLYNLYSKGIRHKDQRLAPPYISIWSVPLHINAINAFIEFGYLARPYTRTIINN
                     HLDDVAEGIAVGIYSLFAGVEVTKKYPYTPYGKKIDLEKYKIDNDNDYFTIVR"
     gene            1023..>1581
                     /locus_tag="PK348_10360"
     CDS             1023..>1581
                     /locus_tag="PK348_10360"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HXK66661.1"
                     /translation="MKKLYAIIILFFSLSSAYAQDTVSEKFVAAAQKASPSVVSIQVF
                     VVEKGHYRKVGYASGTILSSKGYVVTNYHVVSKGTVYQINLADGTECEVESFSDGSIY
                     RVDEKTDIALLKVRHPAGIPLQAIEFEDEKVYPGQWVLAIGNPFGLQQSVTGGIVSSV
                     GRSDIGFADIEDYIQTDVPINPGNSG"
BASE COUNT          487 a          277 c          329 g          488 t
ORIGIN      
        1 aaaggatata tatacagaca ttcatgagca ggggccatca ttaaattcac accttatcca
       61 taaagaacct aacgcaccat acaggctgtt tgactttatt agcaacgatg gcacgcttaa
      121 agaaggcaga ataagttata ttaacagtat taagccacat ctggtggttt caatacattt
      181 tgctttgaat tcgtcaccat actttcgtgg tatgaatgca gtcattgcag caccatacag
      241 tattttatat aagggattgc agtattttca gggtactctt actgacaggt ccttttttta
      301 tcacagtaag tatgctgact ggtttactga agatgaacgt aagtcaggat ttttctggta
      361 ttccaatgat gttgttatgt atttcacagg ctatcggata aagaatgatt atagcattaa
      421 cactgatgca ttcagaggct acaggtataa tatggttcag tgggctttta gtgacccacc
      481 tggctgggca catattgcaa agcatcatcc gcccaaaaca ccgtatgcaa acaatataca
      541 acagtttatt cctcaaaatg ccttttttac ccgtgaacaa agcaaatatg aacagtatcg
      601 ccgtgacgga ggatttgaag gttacggtgg tgacaacctc tatgcatcaa atgagattat
      661 acgatttgtg ctatacaatc tttattcaaa gggcatacgg cataaagatc agcggcttgc
      721 tccaccgtat atatcaatat ggtcagtacc actgcatatc aatgccatta atgcatttat
      781 tgagtttgga tatttagccc gtccatatac ccgcactatt atcaataatc atctggatga
      841 tgttgcggag ggaattgctg ttggaatata ttcacttttt gctggtgtag aagttaccaa
      901 aaagtatccc tacacaccgt atggaaaaaa gattgacctt gaaaagtata aaattgataa
      961 cgataatgac tattttacaa tagtgcgata gctcatagta gtatgtcttc tggagagaaa
     1021 gtgtgaaaaa actgtatgca attatcatac tatttttttc tttatcatct gcttacgctc
     1081 aggataccgt cagtgaaaaa tttgttgctg ctgctcagaa agcaagccca tcggtagtga
     1141 gtatacaggt atttgttgtt gaaaaaggac attatcgaaa agttggttat gcatcaggca
     1201 ctatcctttc atcaaaaggg tatgttgtta ccaattatca tgttgtttca aaaggtactg
     1261 tatatcagat taatttagcg gatggcacag aatgtgaggt tgaatcattt tctgatggaa
     1321 gtatctacag agtagatgaa aaaactgata ttgcactgtt aaaggtacgg catcctgccg
     1381 gcattccttt acaggcaata gagtttgaag atgaaaaagt atatcctggt cagtgggtac
     1441 ttgcaatagg taatcctttt gggctgcagc aatcggtaac aggtggtatt gtatcgtcag
     1501 tggggcgcag tgacataggc tttgctgata tagaagacta tattcagact gatgtcccta
     1561 ttaatccagg taattcaggt g
//
LOCUS       DAOSZP010000555         1580 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_57923_length_1580_cov_2.963934, whole genome shotgun sequence.
ACCESSION   DAOSZP010000555 DAOSZP010000000
VERSION     DAOSZP010000555.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1580
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57923_length_1580_cov_2.963934"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>504)
                     /locus_tag="PK348_10365"
     CDS             complement(<1..>504)
                     /locus_tag="PK348_10365"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HXK66662.1"
                     /translation="GVIFGKFNLEELYSKLGINKETIKMSEFADIFSESRAFTEKEKQ
                     LIQKSVDYTYNEFVQKVETGRNIPQESIPSVAEGKVFTGAQALQNKLADKEGGLIAAI
                     SYAATLAHIKGEFQVVQLPQKDIDLRKLLKGTSFQASLSGILQYVINALALETLSREE
                     ALLLYPY"
     assembly_gap    505..514
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<515..>1580)
                     /locus_tag="PK348_10370"
     CDS             complement(<515..>1580)
                     /locus_tag="PK348_10370"
                     /inference="COORDINATES: protein motif:HMM:NF013506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HXK66663.1"
                     /translation="LSIVLRDQVKEVKDKSLFEPSKASFFDIVRSLREAMYDESIAGI
                     LIQIDAPALNFAQAQELREEIKRCKQKGKPVYCILASMGNIEYYIASSASKIYYVPNQ
                     PFGITGLKAEVYFFKGLFDKVGIRFEEIKKGQYKSFGESFTRQQMSDAARENIISLLK
                     DLNQQYIQDIAQDRNISVQEVEQCIAGGILTPQLAKSRGFIDDIARPYEVLQRLGVER
                     YGLSYLVTCDSYIEETMFVYEWGPKPKIAVIYVEGNIIRGEAKDDSFLSPAMIGDSNY
                     RKYLEKVFADKDVKAVVIRINSGGGSAIASDLMWHTLVAMKKRYKKPVVISFGNIAAS
                     GGYYIACTNDTIFTSKATITG"
BASE COUNT          442 a          370 c          250 g          508 t
ORIGIN      
        1 tcatagggat acaacaacaa tgcttcttca cgggaaagtg tttctaatgc caacgcattt
       61 attacatact gtaatatgcc tgataatgat gcctgaaagc tagtcccttt cagtaacttc
      121 cgcaagtcaa tatctttctg cggcaactgc acaacctgaa actccccttt gatatgagca
      181 agcgttgcag cataggatat tgctgctata agcccacctt ccttatcagc aagcttgttc
      241 tgcaaagcct gggcaccggt aaatactttc ccttcagcta ccgacgggat gctttcttgt
      301 gggatattcc ttcccgtttc aactttttga acaaactcat tataggtata atccacactc
      361 ttctggatta gctgtttttc tttttctgtg aaagctcgtg attcagaaaa aatatctgca
      421 aattcgctca tcttgattgt ttctttattg atgcctaact tgctgtacaa ttcctctaaa
      481 ttaaattttc caaatataac tcctnnnnnn nnnnccggta atggttgctt tgctggtaaa
      541 gatagtatca tttgtacagg ctatataata acctcctgaa gcagcaatat tcccaaatga
      601 tataactacc ggttttttat agcgcttttt cattgccaca agtgtatgcc acatcaaatc
      661 agaagcaatt gccgaacctc ctcccgaatt aatgcggatt accactgcct taacatcttt
      721 atcagcaaat accttttcaa gatattttcg atagttacta tcgcctatca ttgctggtga
      781 cagaaaacta tcatctttag cttccccacg tataatattt ccctcaacat agataacagc
      841 aatctttggt ttgggtcccc actcatatac aaacattgtt tcttcaatat aactatcgca
      901 tgtaaccagg tatgacaggc catatcgttc aacacctaat cgttgtaaca cttcatacgg
      961 ccgtgcaatg tcatcaataa atccccttga ctttgctagc tgtggtgtta atataccgcc
     1021 tgctatacat tgctccactt cctgaacaga gatatttctg tcctgagcaa tatcctgtat
     1081 gtactgctga tttaaatctt tcagcaatga aataatattt tccctggcag catctgacat
     1141 ctgctgccgt gtaaacgatt cgccaaatga tttatactga ccttttttaa tctcttcaaa
     1201 cctgatacca actttatcaa acagcccttt gaaaaagtac acctcagcct taaggccggt
     1261 aataccaaaa ggctggttgg gaacataata tattttagat gctgacgaag caatatagta
     1321 ttcgatattg cccattgatg caaggatgca gtataccggc ttcccttttt gcttacatcg
     1381 cttgatttct tctctcagtt cctgtgcctg tgcaaaattt aaagctggtg catctatctg
     1441 aataagtata cctgcaatac tttcgtcata cattgcctca cgcagtgagc gcacaatatc
     1501 aaaaaaagat gcttttgatg gctcaaataa gcttttgtcc ttaacctctt taacctgatc
     1561 ccgcaggact atcgacaaat
//
LOCUS       DAOSZP010000556         1580 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_57935_length_1580_cov_2.428197, whole genome shotgun sequence.
ACCESSION   DAOSZP010000556 DAOSZP010000000
VERSION     DAOSZP010000556.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1580
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57935_length_1580_cov_2.428197"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1580)
                     /locus_tag="PK348_10375"
     CDS             complement(<1..>1580)
                     /locus_tag="PK348_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010959466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HXK66664.1"
                     /translation="GDRKNMAYNGTIVTYGRGSGIAVATGMNTELGKIATMLQEEEEV
                     KTPLQKRLAVFGKKLSIAVLVLCAVIFGIGLLRGEEPLRMLLTAISLAVAAIPEALPA
                     VITISLALGAKKMVNQNALIRKLPAVETLGSVTYICSDKTGTLTQNKMTVETIFINNT
                     LFTATQPLQDNHSTSLQLFFYALALNNDAIRDASGTLLGDPTETALYHYAATNSFDKA
                     LLTQTLPRVAEIPFDSERKCMTTIHKNTKQDITREPYIVFTKGAVDTIIDITDTIMIN
                     SEIVPINKDEILKVTEKLSEQGLRVLGIATKFSDTLPSSISPETVESNLTLLGLVGMI
                     DPPRQEAHQAVSECKSAGIKPVMITGDHPLTAKAIAQRLSIIDENRPYIITGKELQEL
                     SLEEFEQKVEYISVYARVAPEQKLKIVKALQDKGQFVAMTGDGVNDAPALKRADIGVA
                     MGITGTDVSKQAAHMILLDDNFATIVKAVKEGRRIFDNIRKFIKYTMTSNSGEIWTIF
                     LAPFFGLPIPLLPIHILW"
BASE COUNT          428 a          308 c          320 g          524 t
ORIGIN      
        1 ccataaaata tgtataggga gcaatggaat gggtaaacca aaaaatggtg ctaaaaatat
       61 tgtccatatt tcacctgaat tactagtcat ggtatatttt ataaattttc tgatattgtc
      121 aaagatcctt cttccttcct tgactgcctt tacaatggtt gcaaaattat catcaagtaa
      181 aatcatgtgt gcagcttgct ttgacacatc ggttccggtt attcccatgg caacacctat
      241 atctgcccgc ttcaaagcag gtgcatcatt aacaccatcg cctgtcattg caacaaactg
      301 gcctttatcc tgtaatgctt ttacaatctt tagcttttgt tcgggtgcaa cccttgcgta
      361 aacgctaatg tattccactt tttgttcaaa ttcttcaagc gatagctcct gcaattcttt
      421 gcctgttata atataggggc gattttcatc aataatacta aggcgttgtg ctatagcttt
      481 ggctgttagt gggtggtcac cggttatcat gactggtttg atgcctgctg atttacattc
      541 agacacagct tggtgtgcct cctggcgggg agggtcaatc atgccaacaa ggcctaacaa
      601 tgtaagattt gattcaaccg tttcaggtga tattgatgaa ggaagagtat cagaaaattt
      661 tgttgcaatt cccagtactc taagtccttg ttctgaaagt ttttcagtca cttttaaaat
      721 ttcatctttg ttaatgggta ctatctctga attaatcatg atggtatcag ttatatcaat
      781 gattgtatcc acagcacctt tggtaaatac tatgtatggt tccctggtta tatcttgttt
      841 agtgttttta tgaatggtgg tcatgcactt tctttcagaa tcaaatggta tttctgccac
      901 acgggggagt gtttgagtaa gcaatgcttt atcaaacgaa ttagtagcgg catagtgata
      961 cagtgcagtt tcggttggat cacctaacag tgtgccagat gcatcacgta tggcatcatt
     1021 attaagagcc agagcataga aaaatagctg taaacttgtg ctgtgattgt cttgaagtgg
     1081 ttgagttgca gtaaataatg tgttattaat aaaaatagtt tcaacagtca tcttgttttg
     1141 tgtaagcgta cccgtcttat ctgaacagat atatgtaacc gatcccaatg tttctacagc
     1201 aggaagtttg cgtataaggg cgttttgatt caccattttt tttgcgccaa gagccagtga
     1261 aatggtgata actgcaggca gtgcttcggg tattgctgca acggcaagcg aaattgcggt
     1321 caaaagcatg cgcagtggtt cttcgccacg taaaagacca atgccaaaga taactgcgca
     1381 gagaacaaga actgctatag aaagcttttt gccaaaaaca gctaaacgct tttgcaatgg
     1441 tgtttttacc tcttcttctt cctggagcat tgttgcaatt ttaccaagtt cagtattcat
     1501 gcctgttgca acagcaattc cacttcctct tccatacgtc acaattgtgc cgttatatgc
     1561 catgtttttt cggtcaccca
//
LOCUS       DAOSZP010000557         1578 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_58032_length_1578_cov_3.163493, whole genome shotgun sequence.
ACCESSION   DAOSZP010000557 DAOSZP010000000
VERSION     DAOSZP010000557.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58032_length_1578_cov_3.163493"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1578)
                     /gene="nifJ"
                     /locus_tag="PK348_10380"
     CDS             complement(<1..>1578)
                     /gene="nifJ"
                     /locus_tag="PK348_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014062313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HXK66665.1"
                     /translation="RVVEMQSEAGAAAAVHGSLQAGSLTTTYTASQGLLLMIPNMYKI
                     SAELLPGVFHVSARSISAQALSIFGDHQDINACRQTGFAMLASGGVQEVMDLAAVAHL
                     SAIKSRIPFLHFFDGFRTSHEIQKIEVLNYDDLAKLIDYAKLEEFRNNALNPEHPVTR
                     GTAQNPDIYFQAREVSNIFYQAVPDIVEEYMQEITKLTGREYHPFTYYGAPDAEYIIV
                     AMGSVTETIEETIDYLIKKGEKVGLVSCHLYRPFSPKYFFKVLPQTVKRIAVLDRTKE
                     PGSIGEPLNLDVRAAFYGTANPPVIVGGRYGLSSKDTTPAQILAVYNNLKQSEPKNGF
                     TIGIVDDVTFTSLPLPEDTSVVPEGTFEGKFFGVGSDGTVGANKNSIKIIGDNTDLYA
                     QAYFAYDSKKSGGVTVSHLRFGKKPIRSTYLVNNPDFVACHVPAYLDKYDILKGLKKG
                     GTFLLNSLWDEEETKKRLPDHIKRYLAENNIKFYIINATEISERIGLPGRTNTIMQAA
                     FFKVSNVIPYEIAEKEM"
BASE COUNT          459 a          326 c          297 g          496 t
ORIGIN      
        1 ttcatttctt tttcagctat ttcataggga atgacattac taaccttgaa aaaggccgcc
       61 tgcatgatgg tattggtcct tcctggaagg ccaatgcgtt cagatatttc cgttgcgtta
      121 atgatataaa atttgatgtt gttttcggct aaatatcgtt taatatgatc cgggagcctc
      181 tttttggttt cttcctcatc ccacaacgaa ttgagtaaaa atgttccacc ttttttcaaa
      241 ccttttaaaa tatcatattt atccagatat gcaggaacat ggcaggctac aaaatcagga
      301 ttgttaacta agtatgttga tcgtataggt tttttgccaa atctaaggtg ggaaaccgtc
      361 acgccgcctg atttctttga atcgtatgca aagtatgcct gtgcatataa atcagtgttg
      421 tcaccaataa ttttgattga gttcttgttt gcaccaacag taccatcaga tccaacacca
      481 aaaaacttac cctcaaatgt accttccgga accacactgg tatcttcagg cagtggcaat
      541 gatgtaaagg ttacatcatc cacaatgcca atagtaaaac cattctttgg ttcactctgt
      601 ttgagattat tatacactgc aagaatctgt gcaggtgtag tatctttgga gcttaatcca
      661 tatcgtccac caactataac aggtggattg gctgttccat aaaatgctgc acgaacatct
      721 aaatttaatg gttcaccaat ggatccgggt tctttggttc tatcaagcac cgcaatacgc
      781 tttactgttt gtggcagtac tttgaagaaa tactttggtg agaatggcct gtacagatgg
      841 catgaaacaa gtccaacttt ttcacctttc ttgattaaat aatctattgt ctcttctatt
      901 gtttctgtga ctgagcccat agcaacaata atgtattcag catcaggtgc accataatag
      961 gtgaagggat ggtattccct gccagtaagt tttgttattt cctgcatgta ttcttcaaca
     1021 atatcaggta ctgcctgata aaagatattg ctgacttccc tggcctggaa ataaatatcc
     1081 gggttttgtg ctgtaccacg tgtaacggga tgttcgggat ttagtgcatt gtttctgaat
     1141 tcttccaact tggcataatc aatgagcttt gctaaatcat catagtttaa tacttcaatt
     1201 ttctgaattt catgagaggt tctgaaacca tcaaagaaat gcaaaaatgg tatacgtgat
     1261 ttaattgctg aaagatgtgc aacagcagct aaatccataa cttcctgcac accaccgctt
     1321 gcaagcatcg caaaaccagt ttgacggcac gcatttatat cctgatgatc accaaatatt
     1381 gataaggcct gagcagaaat agaacgtgca ctaacatgaa atacacccgg tagtaattca
     1441 gcagatattt tatacatatt gggaatcatt aacagcaatc cctgcgatgc tgtatacgta
     1501 gtggtaagcg aacctgcctg tagtgatcca tgcactgcag cagcagcccc agcttctgat
     1561 tgcatctcta caacacga
//
LOCUS       DAOSZP010000558         1569 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_58472_length_1569_cov_4.142668, whole genome shotgun sequence.
ACCESSION   DAOSZP010000558 DAOSZP010000000
VERSION     DAOSZP010000558.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1569
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58472_length_1569_cov_4.142668"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..490)
                     /locus_tag="PK348_10385"
     CDS             complement(<1..490)
                     /locus_tag="PK348_10385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HXK66666.1"
                     /translation="MEQFKEIMKGSTLIAKKELSGYFSTPIGYIVISVFLVIAGFLFF
                     STFFLYGQAELRAYFELLPLLFAFVIPAITMRLFAEERNTGSFEMLMTLPLSTIQVVT
                     GKILAATLFASIMISPTLVYAVSVAFVGSPDIGPVIGGYVGAVLLAAAYASIGVFASS
                     MTK"
     gene            complement(501..1427)
                     /locus_tag="PK348_10390"
     CDS             complement(501..1427)
                     /locus_tag="PK348_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013243311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXK66667.1"
                     /translation="MISVRNLVKHYGEVKAVDGISFTIRKGEITGLLGPNGAGKTTTL
                     RILTGYLQPTSGMVSVDEFLVNENPIEIKKRIGYLPESAPLYPDMMVYDFLQFMADIR
                     HIYDKEKIKDVAIQCGITEVMHKNINELSKGYKQRVGLAQAILHDPDILILDEPTNGL
                     DPNQIVEIRDLIKELGKEKTVILSTHILQEVEATCNRVIIIDKGSIVADDSTTELKSA
                     RGKDIRINCIIGGTTFEGIKNALKNISGVKNVQHVRDEGQLCYIQVLTGADVDLRPAV
                     FNCVKDSGWTLYEMNREYRSLEHVFRELTVGM"
BASE COUNT          471 a          336 c          260 g          502 t
ORIGIN      
        1 ttttagtcat tgatgaagca aacacaccaa ttgatgcata cgcagcagcc agaagcactg
       61 cacccacata gccgccaata acagggccaa tgtcaggtga accaacaaat gcaaccgata
      121 cagcatagac cagtgttggc gatatcatga ttgatgcaaa cagtgttgct gctaatatct
      181 ttcctgtaac tacctggatt gttgaaagcg gtagcgtcat gagcatctca aacgaaccgg
      241 tattgcgctc ttcagcaaac aggcgcatgg tgattgccgg tatcacaaaa gcaaataaaa
      301 gtggtagcag ttcaaaataa gcccttagtt cagcctgtcc atataaaaag aatgttgaaa
      361 agaaaaggaa ccccgctata accagaaaca cagaaattac aatatatcct attggtgttg
      421 aaaaataccc tgacagttcc tttttggcaa tgagtgttga ccctttcatt atctctttaa
      481 attgctccat acatccctcc ttacataccg acggttagtt cgcggaatac atgctccagg
      541 cttctgtatt cccggttcat ttcatataat gtccagccac tgtcttttac acaattgaat
      601 acagcaggac gcaaatcaac atcagctccg gttaaaactt gaatgtagca taactggcct
      661 tcatcacgta catgttgtac gttttttact cctgatatat tttttaaagc attctttatt
      721 ccttcaaatg tagtgccacc aataatacag tttattcgaa tatcttttcc tctggcactt
      781 tttagctcag tggttgaatc atcagctact atcgaaccct tatcaattat tatcacacga
      841 ttgcaggttg cttccacttc ctgcaatata tgggttgaca gtattactgt tttttccttt
      901 cccagctctt taataagatc acgaatttca actatctggt ttggatcaag gccgttagtt
      961 ggttcatcca gtataagaat atcaggatca tgtaatatag cctgcgctaa tccaactcgc
     1021 tgtttatatc ctttagaaag ctcattaata ttcttgtgca ttacttcggt tatgccacat
     1081 tgaattgcaa catcttttat tttttcttta tcatatatat gcctgatatc agccataaat
     1141 tgaagaaagt catataccat catatccgga tacagaggtg cagattcggg caaatatcct
     1201 atacgttttt taatttcaat tggattttca ttaacaagaa actcatcgac acttaccatt
     1261 ccactggttg gctgaagata accagttagt atacgcagtg ttgtggtttt acctgcacca
     1321 tttggcccca gtaaaccggt tatctcaccc ttcctgattg tgaatgaaat accatcaaca
     1381 gcttttactt cgccataatg ctttaccaga ttccgtacgc ttatcatgta ccgctatcct
     1441 ccttgataaa ataggattgt gattttgttg ttatttttca ttccaaaata tagcgatgaa
     1501 aatatttttt caatatacta caatgtatat attgatgtga tactagaaat atgaaaaaca
     1561 aatgatgaa
//
LOCUS       DAOSZP010000559         1561 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_58903_length_1561_cov_3.481408, whole genome shotgun sequence.
ACCESSION   DAOSZP010000559 DAOSZP010000000
VERSION     DAOSZP010000559.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1561
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58903_length_1561_cov_3.481408"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..961)
                     /gene="amrS"
                     /locus_tag="PK348_10395"
     CDS             complement(<1..961)
                     /gene="amrS"
                     /locus_tag="PK348_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HXK66668.1"
                     /translation="MKEALYYTKHSEDAVQCVLCPHNCIIKEGKSGICNARQNKGGVL
                     YSLIYEKPCATHVDPIEKKPLYHFLPGSRSFSLGTVGCNLQCMHCQNWELSTSSPNDV
                     FCHTLTADDCVKLAIQYECQSISYTYNEPSINIEYVLETAIKAHDKGIKNVMVTNGFI
                     NEEPLRQLYKHIDAANVDLKAFDDEFYKKICKARLEPVLKTLTILKEMNVWIEVTNLV
                     IPTHNDDVTMIQKLVDWFVHNLGTDVPLHFTAFYPAHKLTNVPPTPLRVLKQAYDIAY
                     KAGIKFIYLGNVRERSVTRCPSCGRDLIVRSGFSVMRTNLHDGK"
     gene            complement(986..>1561)
                     /locus_tag="PK348_10400"
     CDS             complement(986..>1561)
                     /locus_tag="PK348_10400"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXK66669.1"
                     /translation="IDENYVIGYMNLGVIYEKRGQYDKALEMYKMAVSKYPNYILGLR
                     NLGIGYRNLNRLDEALDAFFKALKLKKDDAQTLSEIGYVYLLKNDYNKAINYFKQSLQ
                     YDPSYRYSLANLGWCLVQQGKYNDAIPYLEKAHNDNRDYAWAAGYLGWLYENKIQDYN
                     KALYWYGEALKRDPNNQDYRNSYNRLSGYYR"
BASE COUNT          426 a          323 c          307 g          505 t
ORIGIN      
        1 attttccatc atgaagattg gtgcgcatca cgctgaatcc gctgcgaaca ataagatcac
       61 gaccgcatga gggacagcgg gtaaccgagc gttcgcggac attgcctaaa tatataaatt
      121 taatacctgc tttatatgca atgtcatacg cctgtttcag caccctgagt ggtgtagggg
      181 ggacatttgt tagtttgtgt gcagggtaaa aggctgtaaa atgaagtgga acatcggtac
      241 ctaaattgtg aacaaaccag tccacaagct tttgaatcat ggttacatcg tcgttgtgtg
      301 taggtataac caggtttgta acctcaatcc atacattcat ttctttaaga atggttaatg
      361 ttttcagtac tggttccagc cgtgccttgc atattttttt ataaaattca tcatcaaaag
      421 ctttcaggtc aacgtttgct gcatcaatat gcttatataa ctgtcgcaag ggttcttcgt
      481 tgataaatcc gttggtgacc atgacattct taatgccttt atcatgagct ttgatggctg
      541 tttcaagaac gtactcaatg tttatactgg gttcattata ggtgtatgat attgactggc
      601 attcatactg aattgccagt ttaacgcagt catctgctgt gagcgtatgg caaaacacgt
      661 cgttggggga gcttgttgac agttcccagt tctggcagtg catgcattgt aaattacagc
      721 ccacagtgcc aagggaaaaa gacctgcttc ccggcaaaaa atggtacagg ggcttttttt
      781 ctattgggtc aacatgggta gcacagggct tttcataaat taacgaatac agtacgcccc
      841 ctttattctg tcgggcattg caaataccac tctttccttc cttgatgata cagttgtgag
      901 gacacagcac acactgtacc gcatcctcac tgtgttttgt ataatacaat gcttctttca
      961 taacagtata ctgatactgt atagtttagc gatagtaacc tgataaacgg ttatatgaat
     1021 tgcgatagtc ctgattatta ggatcacgct ttagtgcttc gccataccag tacaatgcct
     1081 tattgtaatc ctggattttg ttttcatata accagcccaa atatcctgcc gcccatgcat
     1141 aatctctgtt gtcattatgt gctttctcta aatacgggat tgcatcatta tatttgccct
     1201 gctgtaccaa acaccaccct aaattagcaa ggctgtaacg gtacgaagga tcgtattgta
     1261 acgactgttt gaaataattg atagctttgt tgtaatcgtt ttttagcaga tacacatacc
     1321 caatctcact caatgtttgt gcatcgtctt tttttagttt taatgcttta aaaaacgcat
     1381 caagggcttc atcaagtcta ttcaaattac ggtagcctat tcccaggtta cgcagcccca
     1441 gaatatagtt ggggtatttt gaaacagcca ttttatacat ttccagtgcc ttatcatact
     1501 gaccacgttt ttcataaata acacccaggt tcatgtagcc aatgacatag ttttcatcaa
     1561 t
//
LOCUS       DAOSZP010000560         1553 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_59315_length_1553_cov_3.151535, whole genome shotgun sequence.
ACCESSION   DAOSZP010000560 DAOSZP010000000
VERSION     DAOSZP010000560.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1553
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59315_length_1553_cov_3.151535"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="PK348_10405"
     CDS             <1..339
                     /locus_tag="PK348_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013621421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HXK66670.1"
                     /translation="GMLSPEMKEEITSTGEVKQVFKISKVGTVAGAIVLTGRLKRSDN
                     IRLIRDGVVVYDGKLNSLKRFKNDAGEVEAGQECGFTVENFNDIKEGDNFESYQIVEI
                     KKKLEDSSRG"
     gene            349..723
                     /gene="rbfA"
                     /locus_tag="PK348_10410"
     CDS             349..723
                     /gene="rbfA"
                     /locus_tag="PK348_10410"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HXK66671.1"
                     /translation="MGFRKQKLETQIKKLVGTLIVTEIKDPRIGFVTVTNVELSKDYA
                     YADVYVSVLGDENNKKKSMAGLQSARGFIQYRVGKALSIRTIPEIRFHLDTSIEEGVD
                     MVNLLEKLEKESSKNNSGSSDT"
     gene            727..>1553
                     /gene="truB"
                     /locus_tag="PK348_10415"
     CDS             727..>1553
                     /gene="truB"
                     /locus_tag="PK348_10415"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HXK66672.1"
                     /translation="MSVPHIKDCVLLIDKPSGYTSFDIIHRIRKTLKVKKCGHAGTLD
                     KFASGLLIVCLGWATRLVPFVMGMDKRYIATMQLGVATDTHDSEGQVISKSNIQCTVE
                     DIQSCLQTYFTGEIMQVPPDYSAIKINGKRSSDRVRKGELVAIAPRPVHIHKITVIDF
                     DTACSQLTLDITCSKGTYIRALARDLGLRLHTGAHVIALRRIAIGDFSVTDALTVEQC
                     IELSHKNSEDMRGIVTPFRALGNFGVIAVKPDSLIKIKHGKLLGYDDITHVAGEPVF"
BASE COUNT          500 a          242 c          383 g          428 t
ORIGIN      
        1 ggtatgctgt cgcctgaaat gaaagaggag attacatcaa caggtgaggt taaacaggtt
       61 ttcaaaatca gcaaggttgg aactgtggca ggagctattg tgttaacggg gcgattaaag
      121 cgaagcgata acatcagact gattcgtgat ggtgttgttg tgtatgatgg taagcttaat
      181 tcattgaaac gttttaaaaa tgatgcaggt gaggttgaag caggtcagga atgtggtttt
      241 acggtagaaa atttcaatga tatcaaagaa ggcgataact ttgaatcata tcagatagta
      301 gaaataaaaa agaaattaga ggattcctca cggggataaa ccgccaacat gggattcaga
      361 aaacagaaat tagaaacaca gattaaaaaa cttgttggaa cgttaatagt aacggagatt
      421 aaagatccac gtataggatt tgtaacagtg acaaatgttg aattgagtaa agattatgca
      481 tatgcagatg tgtatgtttc agtgctgggt gatgaaaaca ataaaaagaa gtctatggca
      541 ggtctgcaat ctgcgcgtgg gttcatccag tacagagttg ggaaagctct gagtatccgc
      601 actattccag aaattcgttt ccatttagat acatctatag aagagggtgt ggatatggtg
      661 aaccttctgg agaaattaga aaaagaatct tcaaagaata atagcggaag cagtgatacg
      721 taatatatga gtgttccaca tataaaggat tgtgtgttgc ttattgacaa gccttctggc
      781 tatacttctt ttgatattat acatcgtatc agaaaaacac tgaaagtgaa aaagtgtggc
      841 catgcaggta cgctggataa atttgcatca ggtcttttaa ttgtatgcct gggatgggca
      901 acgcggctgg tgccttttgt tatgggtatg gacaagcggt atattgccac aatgcaatta
      961 ggcgtggcaa ctgacacaca tgacagtgag ggtcaggtta tttctaaaag caatattcag
     1021 tgcacagttg aggatataca atcatgcctg caaacctatt ttacagggga aattatgcag
     1081 gttcctcctg attattccgc cattaaaatt aacgggaagc gttcatcaga tagggtgcgc
     1141 aaaggggaat tggtagcgat agctccaaga ccagtccata ttcataaaat tacagtgata
     1201 gattttgata cagcgtgttc acagcttaca cttgacatta catgtagcaa ggggacctat
     1261 atcagagcac tggctcgaga tctgggtttg cgattacaca caggggcaca tgttatagcg
     1321 ttgcgaagaa ttgccattgg ggattttagc gttactgatg ctctaacagt ggaacaatgc
     1381 atagagctat cgcacaaaaa tagtgaggat atgcgcggaa ttgttacacc tttcagggca
     1441 cttggtaact ttggtgttat agcagtaaaa ccagattcat tgataaagat taaacatggc
     1501 aagctacttg gctatgatga tattacccat gttgccggtg agcctgtatt cag
//
LOCUS       DAOSZP010000561         1553 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_59337_length_1553_cov_2.452603, whole genome shotgun sequence.
ACCESSION   DAOSZP010000561 DAOSZP010000000
VERSION     DAOSZP010000561.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1553
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59337_length_1553_cov_2.452603"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..451
                     /locus_tag="PK348_10420"
     CDS             <1..451
                     /locus_tag="PK348_10420"
                     /inference="COORDINATES: protein motif:HMM:NF014788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HXK66673.1"
                     /translation="NDHEKRLFEAHCLPRPHIEEGLFLANSGVVHSCIDISDGLSSDL
                     MHIAEESNVGFILQEDAIPVSPDLLHYCKEHGYSAIEYALAGGEDYVLVCTADAKKAT
                     KLENDFYKTFKRILYRIGTITTEKKYLMKKNDGTFSDIKPKGWDHFA"
     gene            complement(570..>1553)
                     /locus_tag="PK348_10425"
     CDS             complement(570..>1553)
                     /locus_tag="PK348_10425"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HXK66674.1"
                     /translation="KKKNIACHGCPVACGKLVDVPYGTYTGTNVKMPEYETIYAFGSM
                     LENSDVVSIYNANTVCDEMGMDTISFGVTLAFLTECVEKGLIKNSDLEIPLHFGKWNH
                     VADIAEYAAYQKGLLGKLLAMGSRRLSEYIGDESEKYLYEVKGLEIAGHSARGLRQMS
                     LGYATATRGGSHHDTRPTYYPNDPEVDPGFTHQPEYSFNSQNNTAIGDSLVLCRFIHE
                     RSFGTQINETLAEALSYVTGWDITKQELVEIGERIYTLERLINVSRGIDRQSDVLPYR
                     VMNEPIPDGPSKGWMCTNEDLQKMLDEYYRLRGWDKNGIPTQETLKRLKIY"
BASE COUNT          477 a          290 c          293 g          493 t
ORIGIN      
        1 aaatgatcat gaaaaaagac tgtttgaagc acattgtttg ccgcgtccac atatagaaga
       61 aggtttgttt ttggcaaact ctggtgtagt tcactcatgc attgatataa gtgatggatt
      121 aagttcggat ttaatgcata tagcagaaga aagtaatgtg ggttttatac tgcaagaaga
      181 tgcaatacca gtatcacctg atttattgca ctattgtaaa gaacatggct atagcgccat
      241 agagtatgca cttgctggtg gtgaagatta tgtattagta tgtactgctg atgcaaagaa
      301 agcaacaaaa ctagaaaatg atttttataa aaccttcaaa aggatattat acagaattgg
      361 tacaattacc acagaaaaaa aatatctgat gaaaaagaac gatggtacat tcagtgatat
      421 aaaaccaaaa ggctgggatc actttgcgta agatactata tttttggtgc aggtgagtaa
      481 acaactataa cgatatgtgc ctatggtcgt catgaacttg cttcaggatc tacgttgtgt
      541 tacaaatggt tttgtcaaaa taatgagagt caataaattt taagacgctt taatgtttcc
      601 tgtgttggta taccattttt atcccatcca cgtaatctat aatactcatc aagcattttt
      661 tgcagatctt cgttagtaca catccagcct tttgatggcc catcagggat gggttcgttc
      721 attacacgat agggcaatac atcactttgt ctatcaatcc cacgagatac gttgataagg
      781 cgttctaatg tgtaaattcg ttcaccaatt tctactaatt cctgttttgt tatatcccat
      841 cctgtaacgt atgataatgc ctcagctaat gtttcgttaa tctgtgtgcc aaaactgcgt
      901 tcatgaataa atcggcataa taccagagaa tcgccaatag cggtattgtt ctgtgaatta
      961 aaggaatatt caggttgatg agtaaatcct ggatctactt cagggtcatt aggataatag
     1021 gtcggtcgtg tatcatgatg gcttccacca cgtgttgcag ttgcatatcc cagactcatc
     1081 tgtcgtagac cacgagcaga gtggccagct atctccaatc cttttacctc atataggtac
     1141 ttttctgatt catcaccaat gtattcagat aatcgtctgg aacccattgc cagtaattta
     1201 cccagaagac ctttttgata tgcagcatat tcagcaatgt ctgctacatg attccatttc
     1261 ccaaaatgca gtggtatttc taagtctgag ttttttatca gccctttttc aacacactca
     1321 gttagaaatg caagagtaac accaaaactt atagtatcca tgcccatttc atcacagact
     1381 gtattagcat tgtaaattga aacaacatcg ctattttcta acatggatcc aaatgcatag
     1441 attgtttcat attcgggcat ctttacattg gtaccggtat aggtgccata gggtacatca
     1501 acaagtttgc cacaagcaac tggacagccg tggcaggcaa tatttttttt ctt
//
LOCUS       DAOSZP010000562         1552 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_59376_length_1552_cov_3.666667, whole genome shotgun sequence.
ACCESSION   DAOSZP010000562 DAOSZP010000000
VERSION     DAOSZP010000562.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1552
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59376_length_1552_cov_3.666667"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..289)
                     /locus_tag="PK348_10430"
     CDS             complement(<1..289)
                     /locus_tag="PK348_10430"
                     /inference="COORDINATES: protein motif:HMM:NF013514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HXK66675.1"
                     /translation="MGAKPDFAIDQEECTKGYNIIAGIDEAGRGSLAGPLSVSMVIYP
                     AEIFSNPPEEILRYINDSKKLPYKKRIQAKDIITHYCLWYDCLLVPPEIIDE"
     gene            complement(296..715)
                     /gene="rplS"
                     /locus_tag="PK348_10435"
     CDS             complement(296..715)
                     /gene="rplS"
                     /locus_tag="PK348_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013430653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HXK66676.1"
                     /translation="MKAIQLVQNEFSKSYRDVNFEVGDTVKVHYRIIEGDKERIQVYE
                     GIVIAIDNKGISKTFTVRRISYDVGVERIFPLHSPRIEKITVVRKGKKRRAKLYFLRE
                     RTGKSAKLKEVRSRKKAIETPATHEVVDNSENTETNA"
     gene            complement(712..1413)
                     /gene="trmD"
                     /locus_tag="PK348_10440"
     CDS             complement(712..1413)
                     /gene="trmD"
                     /locus_tag="PK348_10440"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014356490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009019 - tRNA
                     (guanine-N1-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HXK66677.1"
                     /translation="MKIFKIITLFPEFFESPLQTGLMGKAVEKGLCRFEIINLRQWGE
                     GQYRQCDDYPYGGGSGMVLMPGPLFKAIELHKGDSTVIYPSPSGELLTQELVKKLFLY
                     DSYCFICGQYEGIDQRVIDRFVDYEISVGDYVLSGGEYATLTIIDALCRYIPGFMSNP
                     QSLLEESFENYLLEYPQYTRPREIDGMAVPDILISGNHEKIRQWRLEKSIEKTKAVRP
                     DLYRLYKAKKDEVEQ"
BASE COUNT          415 a          344 c          276 g          517 t
ORIGIN      
        1 tttcatcaat tatttcaggc ggcacaagaa ggcaatcata ccacaaacaa tagtgtgtaa
       61 tgatatcttt tgcctgaatg cgttttttat agggcagctt ttttgagtca tttatataac
      121 gtaaaatttc ttctggggga ttgctgaata tttcagcagg atagataacc atagaaacag
      181 aaagaggccc tgctaaagag ccccttcctg cttcatctat gccagcaata atgttatagc
      241 cttttgtaca ttcttcctga tctattgcaa aatcaggttt tgcacccata cagtgttatg
      301 catttgtttc agtgttttct gaattgtcaa caacctcatg tgttgcaggt gtttcaatgg
      361 cttttttacg ggaacgcact tctttaagtt ttgctgattt accagttcgt tcacgcagaa
      421 aataaagttt tgctcttcgt tttttgccct ttctgaccac agtaattttt tcaattcgtg
      481 gagaatgcag cggaaatatc ctttcaacgc caacatcata ggaaattcgt ctaactgtga
      541 aggttttgct aatgccttta ttatcaatag caataacgat gccttcatat acctggattc
      601 gctctttgtc cccttcaatt atcctgtagt ggactttaac ggtgtcgccc acctcaaaat
      661 ttacgtcacg gtaactttta ctgaattcat tttgtactaa ctgtattgct ttcattgttc
      721 tacctcgtcc ttttttgctt tatacaatct atacagatca ggccttactg cctttgtctt
      781 ttcaatactt ttttccaggc gccattgccg tattttttca tgattgccac ttatcagtat
      841 atccggtact gccatcccgt caatttcacg cgggcgtgta tactgcggat attctaaaag
      901 ataattttca aagctttcct caagcagcga ctgtggattt gacataaatc ctggtatata
      961 tcggcacaat gcatcaataa tggttaatgt tgcatactcg ccgcctgaaa ggacatagtc
     1021 gccaacagaa atttcataat caacaaaacg gtcaatcact cgctggtcta tgccttcata
     1081 ctggccacat ataaaacagt aactatcgta caaaaaaagc ttttttacaa gttcctgtgt
     1141 tagcaattct cctgatggtg acgggtaaat gactgttgag tcacccttat gcaattcaat
     1201 ggctttaaat aagggtcctg gcataagcac cataccgctt ccgccaccat acggataatc
     1261 atcacattga cgatactggc cttcgcccca ctgtcgcagg tttattattt caaaacggca
     1321 tagacctttt tcaactgctt ttcccataag cccggtttgc agggggcttt caaaaaattc
     1381 cggaaacagc gtaataattt taaagatctt cactcaataa gcccttcaat agggaaaatc
     1441 tcaagaattc gttcgtgcaa tcgattggtg cttaccatac tttcaacaaa cggtataagg
     1501 tactctttgc cttcgggtgt tttgagtacc agtgtatcgg ctgcaccggt tt
//
LOCUS       DAOSZP010000563         1550 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_59472_length_1550_cov_7.292977, whole genome shotgun sequence.
ACCESSION   DAOSZP010000563 DAOSZP010000000
VERSION     DAOSZP010000563.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1550
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59472_length_1550_cov_7.292977"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..440
                     /locus_tag="PK348_10445"
     CDS             <1..440
                     /locus_tag="PK348_10445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66678.1"
                     /translation="KLNAPFYVKVWAKRGQVDMDNSYALRIVPVQMENLVDVEPNDTK
                     EQANNVQGALIHGYISKKYDIDFYVLHYKGRQKKYFSIEAHAIPISFSVTDALGYILQ
                     TVTVKAGSSSTLHEIVDMKGYCIVKPLNEMYDGLYTIEIKDRP"
     gene            437..904
                     /locus_tag="PK348_10450"
     CDS             437..904
                     /locus_tag="PK348_10450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66679.1"
                     /translation="MNKKRLPIVFFLVLFIASGCKTVPTYEPPIEPIISPTEVNINFR
                     FIAYDTGITNPDEDKRCYYKIFIDKIDAGRTTIGLESQKKYFEAKLSSNMHLIVVEKW
                     VLDEREGEYKKVNNILQPKPNFFYFETKASSLTELILINDKLVNKAQFVVTIQ"
     gene            complement(1036..>1550)
                     /locus_tag="PK348_10455"
     CDS             complement(1036..>1550)
                     /locus_tag="PK348_10455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66680.1"
                     /translation="LPKEEIDKLAQDPAQLERNPLLNKNNFLEQMIQQRLVYKKAIDD
                     GTLKNEELKTLLDISKEGLVVQYYIREKFKNDISIAPQEVEMIYNQQRARFKGVPIDQ
                     AEMYIKQQLFQQKLNMKIKELVDTLRDEKKIEKNMDLLRKELSTQSPIQQAPQQIPQP
                     QQQMQNPPAK"
BASE COUNT          492 a          265 c          289 g          504 t
ORIGIN      
        1 taaagctgaa tgcacctttt tatgttaaag tatgggctaa gcgcggacag gttgatatgg
       61 ataatagcta tgctttgcgg atagttcctg tccaaatgga aaatcttgtt gatgttgaac
      121 ctaatgatac aaaagaacag gctaacaatg tacagggagc tttgatacat ggatatattt
      181 caaagaaata tgatattgat ttttatgttt tacactataa aggacgtcaa aaaaaatatt
      241 tttccattga agcgcatgca atacctatat cattttctgt tactgatgcg ctgggttata
      301 tattacagac agttactgtg aaagcaggta gttcaagtac attacatgag atagttgata
      361 tgaaagggta ctgcattgtt aaaccattga atgaaatgta tgacggacta tatactattg
      421 aaataaagga tagaccatga acaaaaaaag gctgccaatt gtattttttt tggtgttatt
      481 tattgcttca ggatgtaaaa cggtgccaac atatgaacca cctatagagc caatcatcag
      541 tccaaccgag gtgaatatca atttcaggtt tattgcctat gatacaggga ttacaaatcc
      601 agatgaagat aaacggtgct actataaaat ttttattgat aagatagacg ctggcaggac
      661 aaccattggg cttgaatcgc agaaaaagta ttttgaggct aaactatcat ccaatatgca
      721 tcttatcgta gttgaaaaat gggtgcttga tgaacgcgaa ggtgaatata aaaaagtaaa
      781 caatattttg cagccaaagc caaacttctt ttattttgaa acaaaagcct catcattgac
      841 cgagctcata ctaataaatg ataagctggt taataaagca cagtttgttg tgacaataca
      901 ataacaagac aaactgtgct ttatactttt aaaaattaca taattcttat atcatgctac
      961 ctgcatatca cacattcgct cagcagtcac agtaacaaag cactacaatg agtgcaatta
     1021 taacactata acaatctatt ttgcaggagg attctgcatt tgctgctgtg gctgtggtat
     1081 ctgctgtggt gcttgttgta tcggtgattg tgtactcaat tctttgcgca gcaaatccat
     1141 gtttttctct atcttctttt catcacgcaa tgtatcaacc agctctttta tcttcatatt
     1201 gagcttttgc tgaaaaagct gctgctttat gtacatttca gcctggtcaa ttggtacccc
     1261 tttaaaacgg gcacgctgct ggttatatat catctcaacc tcttgtgggg caatggatat
     1321 atcgttttta aacttttcac ggatatagta ttgcactaca agcccttcct tggagatatc
     1381 taaaagagtt ttcaattctt cattttttaa tgtgccatca tcaatggctt ttttatacac
     1441 aaggcgctgc tgaatcattt gctcaagaaa gttgttttta ttaagaagag ggttacgctc
     1501 aagctgagca ggatcctgag ccaatttatc tatctcttct ttaggaagat
//
LOCUS       DAOSZP010000564         1550 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_59500_length_1550_cov_2.496321, whole genome shotgun sequence.
ACCESSION   DAOSZP010000564 DAOSZP010000000
VERSION     DAOSZP010000564.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1550
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59500_length_1550_cov_2.496321"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..137)
                     /locus_tag="PK348_10460"
     CDS             complement(<1..137)
                     /locus_tag="PK348_10460"
                     /inference="COORDINATES: protein motif:HMM:NF013974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66681.1"
                     /translation="MDGNEAAAYVSYAFTEVAAIYPITPSSPMAEHVDEWAAHGKKNI
                     FG"
     gene            532..>1550
                     /locus_tag="PK348_10465"
     CDS             532..>1550
                     /locus_tag="PK348_10465"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HXK66682.1"
                     /translation="MANMYCRDTIADMVRRAALRFGEKTAIAFRDTSLTFRQLEEDCC
                     RFGHLMQQYGIEKGDRVAIIAFNSHYYPISFVGLSKIGAIQVPINYMLNGDEMAYIIN
                     HSKSKMVIVEDNLYNVIAPAKDKCTTVENWLYIPLEGTPTPQGFDNLIEMLQGMPTYD
                     IATTVYPEDIAQIPYTSGTESKPKGAMLSHRALLSQYFSCIIEGEYQPHDISLHALPL
                     FHCAQLHCFLMPFLYIGATNIILHKADPVSMMQLIEKYSITHMFSPPTVWIGIINHPE
                     FKKYNLTSLKKAAYGASIMPVEVLKRLTEVFPGLRLWNYYGQTEMGPVATILKPEDQL
                     KKPGSA"
BASE COUNT          503 a          291 c          263 g          493 t
ORIGIN      
        1 ccaaaaatat ttttcttacc atgagcagcc cattcatcaa catgttctgc cattggtgaa
       61 gatggtgtaa ttggatagat ggcagctact tcagtaaagg catacgatac atatgctgca
      121 gcttcgttac catccattga tttcatgatc ttttttgcca tgaaagcttt ctcctttctg
      181 ttttggtttt aaaatataat aaatcccttc tcagtaataa aactgagatt aattacttaa
      241 taacaatttt tgattaatga aagatcacgg cgtgtatata accttccttc tttagtgtac
      301 tgtaagtcaa taaaaaaatc aattaataaa attctaaata tttaataaaa ttacattatt
      361 tcaattaaca tagcatttat atgcataatt tcaaagtatt cattatgagc gatagttcat
      421 tacatatata ctcttgctat tttaaatttt attaacattt tatgcttgca cgacagaagg
      481 tatatttcat atttgtcata tatcttatca taatataaat ggaggattct tatggctaac
      541 atgtattgca gagatacaat agctgacatg gtgcgacgcg cagccttgcg ttttggcgaa
      601 aaaacagcaa ttgcattcag agacaccagt ttaactttta gacaattaga agaagattgt
      661 tgtcgttttg gtcatctgat gcaacagtat ggcatagaaa aaggtgatcg tgttgccatc
      721 atagcgttta attcacatta ttatccaatc agctttgttg gtctttcaaa gattggggca
      781 attcaggtac ccattaacta catgctcaat ggcgatgaga tggcatatat cataaatcac
      841 tctaaatcca agatggtaat cgttgaagat aatttatata atgttatagc acctgcaaaa
      901 gataaatgca ctacagttga aaactggttg tacattcctc ttgaaggtac tccaacacca
      961 cagggttttg ataaccttat tgaaatgttg cagggcatgc caacatatga tattgctaca
     1021 acggtatatc cagaagacat tgcacagatt ccttatacca gtggcactga atcaaaacca
     1081 aaaggcgcaa tgctatcgca cagagcctta ctgtcacagt atttcagctg tattattgaa
     1141 ggggaatatc aaccacatga tataagcctg catgcactgc cactttttca ctgtgcacag
     1201 cttcattgtt tcctgatgcc atttctgtat ataggagcaa caaatatcat tttacataaa
     1261 gcagaccctg tttccatgat gcaactcatt gaaaagtatt ccatcacaca catgttttcg
     1321 ccacctacag tatggattgg aattatcaat catcctgaat tcaaaaaata taatcttact
     1381 tcacttaaaa aagctgccta tggcgccagt attatgccgg ttgaggtttt aaaacgctta
     1441 actgaagtat tcccgggttt gcgcctgtgg aactactatg ggcaaaccga aatggggcca
     1501 gttgcaacaa tactaaaacc agaggatcag cttaaaaagc ccggttcagc
//
LOCUS       DAOSZP010000565         1547 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_59643_length_1547_cov_2.661528, whole genome shotgun sequence.
ACCESSION   DAOSZP010000565 DAOSZP010000000
VERSION     DAOSZP010000565.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1547
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59643_length_1547_cov_2.661528"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1158)
                     /locus_tag="PK348_10470"
     CDS             complement(<1..1158)
                     /locus_tag="PK348_10470"
                     /inference="COORDINATES: protein motif:HMM:NF040105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXK66683.1"
                     /translation="MKSQNPSQRNYLNDAESLLDQEGYIIIAPDEEKKDLQPEEKQTI
                     KTEHVTTQQMEKLPGKKPEILPKAIEAEATDIPSTIQYLKWHMERIAKNVIGREEIIK
                     QAMYAILTREHMLLLSRTGMAKSYLANYIFNTFDNVRVFASQASKEQTPDNYFGPYNI
                     DEFKKGRIRHNIHGSIIEANLVFLDEFFDASDVVLRSLLSVLNERKFINGPEQIDVAI
                     HTAIATANYMRMNEVTEAILDRFTYKSIIPEDDNVYQQLLIDHTYAFSRGKAIEPDKK
                     IPFNQIIYLSDIVRNKNKEVKIEIPDFIYFMKNVITNKFVSDMRKSDYKFFISPRKQA
                     KLQDFLRANALLNNRFEVTMDDLKDMYLALCTLNSFISVKAKDKSEKDVFLD"
     gene            complement(1170..>1547)
                     /locus_tag="PK348_10475"
     CDS             complement(1170..>1547)
                     /locus_tag="PK348_10475"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXK66684.1"
                     /translation="INTAYPKESPKIKGRKNQLIQALLHIISNAEEAMPNKGEIRINV
                     GSVLKNGEKFASITIEDTGIGIPPENLSKIFQPFFTTKGPKSTGLGLMVTYTIINNHG
                     GAIGVKSAPGEGTSFKILLPAID"
BASE COUNT          402 a          296 c          297 g          552 t
ORIGIN      
        1 atctaaaaat acatcctttt ctgatttgtc ctttgcttta accgagataa aactattcag
       61 tgtgcacagt gctaaataca tatccttaag gtcatccatg gtaacctcaa aacggttgtt
      121 aaggagtgca ttggctctga ggaaatcctg taattttgcc tgtttacgcg ggctaataaa
      181 gaatttataa tcggattttc gcatgtcgct tacaaacttg ttggtgatga catttttcat
      241 gaagtaaatg aaatcgggta tctcaatctt aacttcttta ttcttgttac gcacaatatc
      301 cgacagatag ataatctgat taaatggaat ttttttatca ggttctatcg ccttgcccct
      361 gctgaaagca tacgtatgat caatcagcaa ttgctggtat acattatcgt cttcagggat
      421 aattgattta taggtgaagc ggtctaaaat tgcttcggta acttcattca tacgcatgta
      481 gttggcggtg gctatagcgg tgtgtatagc aacatcaatc tgctcagggc catttataaa
      541 ttttcgttcg ttcaataccg atagcaatga acgcaatacc acatcactgg catcaaagaa
      601 ttcatccagg aaaactaaat ttgcttcaat tattgaaccg tgtatattgt gacggatgcg
      661 tccctttttg aattcatcaa tattatatgg tccaaagtaa ttgtctgggg tttgttcctt
      721 gcttgcctga gatgcaaaaa ctcgtacatt atcaaatgtg ttgaatatat aatttgccag
      781 atacgatttt gccatacctg tccgcgataa cagaagcata tgctcacgtg tcagtatagc
      841 atacattgcc tgtttaataa tttcctcacg cccaattaca ttctttgcaa tacgttccat
      901 atgccattta agatactgta tggtgcttgg aatatcggtt gcttcagcct caattgcttt
      961 gggcagtatt tcgggttttt tgccaggcag cttttccatc tgctgggttg tgacatgctc
     1021 agtttttatt gtttgctttt cttctggctg caaatccttt ttttcttcat caggtgcaat
     1081 gataatatat ccttcctgat caaggagtga ttcggcatca ttgaggtaat ttctttgtga
     1141 agggttttgc gatttcatat agtccccctt tagtcaatag ctggaagtag tattttaaat
     1201 gaagtaccct caccaggggc gcttttaact cctattgctc cgccatggtt gtttataatt
     1261 gtgtaggtga ccatcagtcc caatcctgta gattttggac cttttgttgt aaaaaatggc
     1321 tgaaatatct ttgaaagatt ttcaggagga atgccaatgc cggtgtcttc aatggtaatg
     1381 ctggcaaatt tttcaccatt cttcagtacg ctgccaacat taatgcgaat ttccccttta
     1441 ttgggcattg cttcttcagc attgcttatg atatgcaata aagcctgtat aagctgattt
     1501 tttctgcctt taattttagg gctttctttt ggatatgccg tgttaat
//
LOCUS       DAOSZP010000566         1540 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_59983_length_1540_cov_2.925926, whole genome shotgun sequence.
ACCESSION   DAOSZP010000566 DAOSZP010000000
VERSION     DAOSZP010000566.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1540
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59983_length_1540_cov_2.925926"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(121..333)
                     /locus_tag="PK348_10480"
     CDS             complement(121..333)
                     /locus_tag="PK348_10480"
                     /inference="COORDINATES: protein motif:HMM:NF039402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="HXK66685.1"
                     /translation="MAVTSVRFNAKEEKILKLLQKHYNCDTSSLIKKSLWELYEDIKD
                     KEIIEDFEKKEKSKKARFVSIDNIIQ"
     gene            complement(324..593)
                     /locus_tag="PK348_10485"
     CDS             complement(324..593)
                     /locus_tag="PK348_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66686.1"
                     /translation="MILYEKSVQKAIQKGEIPLRTIQLFHNAFKALDLTKDFSLFDIK
                     KLKGNYKRDYYRLRKGKYRAIFYMENNDFYVIYIGKREEVYDTWQ"
     gene            complement(641..1540)
                     /locus_tag="PK348_10490"
     CDS             complement(641..1540)
                     /locus_tag="PK348_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXK66687.1"
                     /translation="MVENITKRFGDFTAVDRVSFSVAQGEIFGLLGPNGAGKSTTIKM
                     LCGLLQPDEGTGSVGGLDIVHQQWDIKKIIGYMSQKFSLYEDLTIEENLEFYGTVYGL
                     SANLLQQRMNFIYELVNIKDYRHSLVKNLPLGIKQRLALGSALIHDPQILFLDEPTSG
                     VDPLMRRIFWDEIYTLAKEGKTVFVTTHYMDEAEHCNRIALIIAGKIIALDTPQGLKE
                     KLPYTIVKIVTDSYLKAYNVLSKENFVIEVSLFGTDLHVVLEKQYKHFTIIKKLLQSH
                     NIHDVTIEPILPTLEDVFVMSAR"
BASE COUNT          452 a          285 c          257 g          546 t
ORIGIN      
        1 atatactgtc agggaagtgg tagcagcatg atgcaggcag gtgatattgt acggtttgta
       61 cgcaacgctt tgaattgcta attactacat gatactcaca tcacttatat ccagttgagt
      121 ttattgtata atattgtcta tgctcacaaa ccgtgccttt ttactttttt ctttcttttc
      181 aaaatcttct ataatttctt tatccttgat gtcttcataa agctcccata atgatttttt
      241 aataagtgat gatgtatcac aattataatg cttttgtaac agtttaagta ttttttcttc
      301 ctttgcatta aatctcactg atgttactgc catgtatcgt atacctcctc gcgttttcca
      361 atgtaaatta cataaaaatc gttattttcc atataaaaaa tagctctata tttaccttta
      421 cgtaatctat aataatctcg tttgtaattc ccttttagtt tttttatgtc aaataaacta
      481 aagtcttttg ttaaatcaag cgctttgaat gcattgtgaa acagctgtat tgttcttaaa
      541 ggtatttcac ctttctgaat agctttctgt actgatttct catataatat catgtaaaca
      601 atgtattaca tcaggggtgc attgtcaata aaaaatagta ttaccgtgca ctcatgacaa
      661 aaacgtcttc taatgttgga agtatgggtt caatggtaac atcatgaatg ttgtgtgatt
      721 gtagtaattt ttttatgata gtaaaatgtt tatattgttt ttcaagcaca acatgaaggt
      781 cagttccaaa aagagatact tcaataacaa agttttcttt actcagcaca ttgtaagctt
      841 tcaggtaact atcggttaca atttttacaa tagtgtaggg tagtttttct tttaagccct
      901 gtggtgtatc aagggcaata attttgcctg caataataag cgcaatgcgg ttacagtgtt
      961 cggcttcatc catgtaatgg gttgttacaa aaacagtttt accttccttt gccagtgtat
     1021 aaatttcatc ccagaagatt cgtcgcatca aaggatcaac gcctgaggtt ggttcatcta
     1081 aaaaaagtat ctgaggatca tgaatcagtg ccgagcccaa tgcaaggcgc tgcttaatgc
     1141 caagcggcag gtttttcacc aggctgtgcc tgtagtcctt gatatttacc agttcataaa
     1201 taaagttcat gcgttgttgt aaaaggtttg cggaaagccc atataccgta ccataaaatt
     1261 ccagattttc ttcaatggta agatcctcat acagagaaaa tttttgtgac atatagccaa
     1321 ttattttttt tatgtcccac tgctgatgaa caatatcaag ccctcccaca cttcctgtac
     1381 cttcatcagg ctgcaataat ccacagagca tttttatagt ggtgctttta ccagcaccat
     1441 tggggccaag caacccaaaa atttcacctt gagctacaga aaagcttact ctgtcaactg
     1501 cggtaaaatc gccaaagcgc ttggtgatgt tttcaactat
//
LOCUS       DAOSZP010000567         1539 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_60031_length_1539_cov_3.291105, whole genome shotgun sequence.
ACCESSION   DAOSZP010000567 DAOSZP010000000
VERSION     DAOSZP010000567.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1539
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60031_length_1539_cov_3.291105"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1326
                     /locus_tag="PK348_10495"
     CDS             <1..1326
                     /locus_tag="PK348_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012500138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HXK66688.1"
                     /translation="MKLTAWGNYPIVDAQADYLESVESLKNLILSKQKLIAYGNGRSY
                     GDQALNERVIFTKKYNYITDFDSQKGMITCQCGVTLEEILDVIVPKGWFLQVTPGTKY
                     ITVGGAIASDVHGKNHHKEGTFCDHVMSFELMLPDGKVITCSRKNNPEIFKATCGGNG
                     LTGVILKATFRLKKIESAYIKQRTIRAQNIEELINAIEDNEHYTYAVAWIDCVTKGKH
                     LGRGVLLLGEHATVDDLPLKKRNNPLVLPKKKKLTVPIYFPNFTLNKFSIKAFNWLYY
                     HKTPKGVSESIIDYNTFFYPLDAIDNWNRIYGTRGFTQYQFVIPKKAGHEAMRTIMEK
                     ITGSGMGSFLAVLKTAGKHNGNYLSFPMEGYTLALDFAIHKKLFAFLDELDELIMQYG
                     GRLYTTKDVRMSSQFFKASYPDISKFMTIRKKLKANTLFESLQSKRLGI"
     gene            1323..>1539
                     /locus_tag="PK348_10500"
     CDS             1323..>1539
                     /locus_tag="PK348_10500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="short-chain dehydrogenase"
                     /protein_id="HXK66689.1"
                     /translation="MKWMLVLGAESDIAKAVVRRFAKEGVNFYLAHFNIKQIEDFAKD
                     VSIRFGVKTIPVTFDAVKYNTHKAFYDK"
BASE COUNT          529 a          239 c          305 g          466 t
ORIGIN      
        1 atgaaactca ctgcctgggg aaactaccct attgttgatg cacaagcaga ttatttagaa
       61 tcagttgaat ccttaaaaaa cctcattctt tcaaaacaaa agcttattgc ctatggcaat
      121 ggccgtagtt atggcgatca ggcattgaat gagcgcgtta tttttacgaa aaaatataac
      181 tatattacag attttgattc acaaaaaggc atgataacct gccaatgtgg tgtgacgctg
      241 gaagaaatat tggatgttat tgttcccaag ggatggtttt tacaggttac ccctgggaca
      301 aaatacatta cggtgggggg agctattgct tcagatgtcc atggaaagaa ccaccataaa
      361 gaaggtacct tttgcgatca tgttatgtca tttgaactca tgcttcctga tgggaaagta
      421 attacctgtt cacgcaaaaa taaccctgaa atctttaaag caacctgtgg cgggaacggc
      481 ttgacgggtg taattttaaa agctactttc aggctaaaaa agattgaatc agcatatata
      541 aaacaaagaa caatacgtgc gcaaaatatt gaagaactca taaatgcaat cgaagataat
      601 gagcattata cctatgctgt tgcctggatt gattgtgtta caaaaggtaa acatttgggt
      661 agaggagttt tactgttagg tgaacatgca actgttgatg atttgccctt aaaaaaaaga
      721 aataatccac ttgtgttgcc aaaaaagaaa aaattaactg tgccaatata ctttccaaat
      781 tttacattga ataaattttc aattaaagca tttaactggc tctattatca taagacacct
      841 aaaggtgttt cggagtcaat tattgattac aatacatttt tttatccatt ggatgctatt
      901 gataactgga acaggatata tggcacacga ggttttacac aatatcagtt tgtgatacca
      961 aagaaagcag ggcatgaagc aatgcgaaca ataatggaaa aaataacagg tagtggtatg
     1021 ggctcctttt tagctgtatt aaagactgca ggcaaacaca atggtaatta tctctcattt
     1081 cctatggaag ggtatacact ggcgcttgat tttgcaatac ataaaaaatt gtttgcattt
     1141 cttgatgaac ttgatgagct tattatgcaa tatggaggca ggctttatac tacaaaagat
     1201 gttcgtatga gctcacaatt ttttaaagca tcatatcctg atataagcaa atttatgact
     1261 ataaggaaaa aattgaaggc aaatacactt tttgaatctt tacaatcaaa gaggttaggt
     1321 atatgaaatg gatgcttgtg ctcggagcag aatcggatat tgcaaaggcg gtggtgcgaa
     1381 ggtttgcaaa agaaggggta aatttttacc ttgcacattt taatattaaa caaattgaag
     1441 attttgcgaa agatgtatct atccgtttcg gagttaaaac cattcctgtt acttttgatg
     1501 cggttaagta taacacacat aaagcttttt atgataaac
//
LOCUS       DAOSZP010000568         1537 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_60141_length_1537_cov_3.053306, whole genome shotgun sequence.
ACCESSION   DAOSZP010000568 DAOSZP010000000
VERSION     DAOSZP010000568.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60141_length_1537_cov_3.053306"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..989)
                     /locus_tag="PK348_10505"
     CDS             complement(<1..989)
                     /locus_tag="PK348_10505"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: protein motif:HMM:NF015047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HXK66690.1"
                     /translation="MIKTKITEMFGVKYPIICGAMMWLCKPKLCAAISNAGGIGNLTA
                     GNYNTEEEFRNAIRETRKLTDRPFFVNVTILPSVRITGEHHKMYLKVCAEEKVAGIEV
                     SGAPIDKACGMEYIDMLKKAGVKLFHKVGAVRHAVHAEHVGYDGIYAAGIEEGGHPLS
                     DDVTTMVLTTKIAQTVKIPVITVGGIANGKTMAAALMLGAEGVMMASRFIATKECEVH
                     DNIKQELVKRQEYETTLIMKSLHLQGRALKNEVVNKILEIEQRGGGFEELFPLISGER
                     MSKAWEQGDVNAAPLMVGQSIGLIDDIPTCAELLENMNKEAEEQIKKVTGNIK"
     gene            complement(1020..>1537)
                     /locus_tag="PK348_10510"
     CDS             complement(1020..>1537)
                     /locus_tag="PK348_10510"
                     /inference="COORDINATES: protein motif:HMM:NF012339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="iron-sulfur cluster-binding domain-containing
                     protein"
                     /protein_id="HXK66691.1"
                     /translation="FHYIPVLEEGSGTYKGYITAKIIKEVVGDSSDKTFFVCGPQALY
                     EHLLKELKPLTLKPRQLRMEMYGVPSNVFEQPGWPKDISRNSTFVIKIRGKKDITVSA
                     GEPLLQSLEKNGIVVPSLCRSGECSLCRVKLGSGKVYQAEGVKLRQADRRFGYIHSCA
                     AFPISDCEIII"
BASE COUNT          412 a          366 c          238 g          521 t
ORIGIN      
        1 tatttaatat ttccagtaac cttttttatt tgctcttcag cttctttgtt catattttca
       61 agcagttctg cacaggttgg aatatcatct ataaggccaa tggattgtcc aaccattaat
      121 ggtgctgcat tgacatcacc ctgctcccat gccttgctca ttcgttcacc tgatattaat
      181 gggaacaatt cttcaaagcc tccacctcgc tgttcaattt caagtatttt atttacaact
      241 tcattcttta aagcccttcc ttgtaaatgc aggcttttca taataagtgt ggtttcatat
      301 tcctggcgtt tgacaagctc ctgctttata ttatcatgca cttcacattc ttttgtagct
      361 ataaaacggc ttgccatcat tacgccttca gcacctaaca tcaacgctgc tgccatagtt
      421 ttaccatttg caataccgcc aactgtaata actggtattt taaccgtttg tgcaattttt
      481 gtagttagca ccattgtagt tacatcatca ctcaacggat gcccaccttc ttctatccct
      541 gctgcatata ttccatcata tccaacgtgt tcagcatgaa cggcatgacg tactgcaccc
      601 actttatgaa aaagtttgac acctgccttt ttaagcatgt caatatattc catgccacag
      661 gctttgtcaa taggggcgcc tgaaacttca atacctgcaa ctttttcttc agcacacact
      721 ttaagataca ttttgtgatg ctcccctgtg atgcgaacag acggcaatat tgtgacattg
      781 acaaaaaaag gcctgtcagt tagttttcgt gtttcacgga tagcatttct gaattcttct
      841 tcggtattat aattaccagc cgtcagattt cctatcccac ccgcatttga aatagcagca
      901 cacaattttg gtttacatag ccacatcatt gcaccacaaa taatgggata ttttacccca
      961 aacatctcag taatcttggt tttaatcata tagaaactct cctgaaaaaa aatatttatt
     1021 taaattatta tttcacaatc acttatggga aatgctgcac atgaatggat atacccaaaa
     1081 cgcctatctg cctgtctcag tttcactcct tcagcttgat acactttacc actccctaac
     1141 tttactctgc ataaactgca ttcaccgctc ctgcacagtg atggtaccac aatcccattt
     1201 ttttcaagtg actgcagtaa tggttcacct gcactaaccg ttatatcttt tttcccgcgt
     1261 atcttaataa caaatgttga atttctgcta atatcttttg gccacccagg ctgttcaaaa
     1321 acattagagg gaacaccgta catctccatt cgaagctgcc ttggttttaa tgtaaggggc
     1381 tttagttctt tcaacaggtg ttcatagagt gcttgtggtc cgcagacaaa gaatgtttta
     1441 tcggaactat cgcccaccac ttcttttatg atttttgctg taatataccc tttataggtg
     1501 cctgagcctt cttcaagtac ggggatatag tgaaaaa
//
LOCUS       DAOSZP010000569         1520 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_61031_length_1520_cov_3.512628, whole genome shotgun sequence.
ACCESSION   DAOSZP010000569 DAOSZP010000000
VERSION     DAOSZP010000569.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1520)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1520)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1520
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61031_length_1520_cov_3.512628"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..411)
                     /locus_tag="PK348_10515"
     CDS             complement(<1..411)
                     /locus_tag="PK348_10515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66692.1"
                     /translation="MKKNFLTIIMVLFTSVLLAQTTQKLDIPVQSLYSNQVKIKDIYF
                     NRRVDIAGKGDVLEVEVLFENLTDYPMDIYVNIIATYEKEEKTKSSFEMPIPEKERIR
                     NFVPYPDDIANYEYTDSKGKKIFQAYPKNTKSGIN"
     gene            complement(487..1272)
                     /locus_tag="PK348_10520"
     CDS             complement(487..1272)
                     /locus_tag="PK348_10520"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HXK66693.1"
                     /translation="MDYKAITEYAIHVCKQAGAIILEGYYSNDVTVDYKSRTNLVTNI
                     DYESEKLIVESIKNTFPDHGIVAEEGSLKKSSSNYVWYVDPLDATNNFAHKIPHFCVT
                     VALYSREIASTVAGVVYEPLRDECFYAWKGGGAYLNTKRIYVSQIQDIGISIIATGFP
                     YKKDDPDVNNLKQFNNVLPHVQGIRRMGSAAIDLSYVACGRFDGYWEPMLYPWDVAAG
                     ALIVEEAGGMVTKYTGEAFDPEYPEICATNGLIHRQLLDCINA"
     gene            complement(1275..>1520)
                     /locus_tag="PK348_10525"
     CDS             complement(1275..>1520)
                     /locus_tag="PK348_10525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66694.1"
                     /translation="NIDDPYAKFGNGQGWFGINTDKWTKGDPFSGFRSLSHVFTASVE
                     EYIHKNIFLYQSLGFELADTSEDHHEITFKLGAGVEL"
BASE COUNT          455 a          335 c          236 g          494 t
ORIGIN      
        1 attaattcct gattttgtat tctttggata cgcctgaaat atttttttcc ctttactgtc
       61 agtatattca taattggcta tatcatcagg ataaggaaca aaatttcgta tgcgctcctt
      121 ttcagggatc ggcatctcaa agctggattt agttttctcc tctttttcat aggtagcaat
      181 aatattgaca tatatatcca tgggataatc cgttaaattt tcaaacaaca cctcaacttc
      241 aaggacatct cctttccctg ctatatccac tctcctgtta aaataaatat cttttatttt
      301 gacctgattg ctatataaag actgaacagg aatatccaat ttttgagtag tctgtgccag
      361 caatacagat gtaaacaata ccataattat tgttaaaaaa tttttcttca tataaaccgc
      421 ccatcttaca gtgtttctta ttatctatcg gtcaatgtgc cagcaaaatt tagaagaata
      481 caccatctac gcattaatac aatcaagtag ctgacgatga atgagcccat tggtggcaca
      541 tatttcaggg tattcagggt caaaagcctc tccagtatat ttagtaacca tccctcccgc
      601 ttcttccaca ataagtgcac cagctgccac atcccatgga tacagcatgg gctcccagta
      661 tccatcaaac cttccacatg ccacatatga aaggtcaatg gctgccgatc ccattcgccg
      721 tataccctga acatgcggca aaacattatt aaactgtttc agattgttta catccgggtc
      781 atctttctta tatggaaaac cggtagcaat aattgaaatg ccaatatcct gaatctgaga
      841 aacatatatg cgttttgtat tgagataggc accgcccccc ttccatgcat aaaaacattc
      901 atcgcgtaat ggttcataga ccacaccagc aactgttgat gctatctccc tggaatataa
      961 cgcaacagta acacaaaagt gcggaatctt gtgtgcaaag ttattggtag catctaatgg
     1021 atcaacatac catacataat tgctgctgga tttttttaaa cttccctctt ctgcaacaat
     1081 gccatggtca ggaaatgtgt tttttatgct ttcaacaatt aatttttcag attcataatc
     1141 tatattggtt actaaatttg tcctgctttt gtaatcaact gttacatcgt tgctgtaata
     1201 gccttccagt atgattgctc ccgcctgttt gcaaacgtgg atagcatatt cagtgattgc
     1261 tttataatcc atacctacaa ctcaacacct gctccaagtt taaacgtaat ttcatgatgg
     1321 tcctctgatg tatcagccag ttcaaaaccc agtgactggt aaaggaaaat atttttatga
     1381 atatattcct ctacactagc agtaaataca tgtgaaagtg acctgaatcc tgagaaagga
     1441 tcaccttttg tccatttatc tgtgttaatg ccaaaccatc cctgcccatt gccaaatttt
     1501 gcatatggat catctatatt
//
LOCUS       DAOSZP010000570         1515 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_61311_length_1515_cov_2.073288, whole genome shotgun sequence.
ACCESSION   DAOSZP010000570 DAOSZP010000000
VERSION     DAOSZP010000570.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61311_length_1515_cov_2.073288"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            65..418
                     /locus_tag="PK348_10530"
     CDS             65..418
                     /locus_tag="PK348_10530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66695.1"
                     /translation="MYKSEESDIFIHDIIKLHRIAKVIYTLEEVYALLVQLMFRQQLH
                     SLLYSLYETSDMQRSTTAYKGSLRQLYLETGMEIFALPAITHTETIELFRDNTERMHT
                     ILAKAAGFSWLSDNE"
     gene            complement(419..949)
                     /locus_tag="PK348_10535"
     CDS             complement(419..949)
                     /locus_tag="PK348_10535"
                     /inference="COORDINATES: protein motif:HMM:NF024827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c family protein"
                     /protein_id="HXK66696.1"
                     /translation="MAARKNASFVGVAVCKKCHESEAIGNQYKSWQQSPHAKAYLLLK
                     QEKALSLAKELSIENPSDNEKCLACHTTGLGKNPALWEDGVGCESCHGPGSMYNSYEN
                     HVALGNREVAYKKALSFGMYPILGTDGIKAREKVCRSCHNEKRPCFPKDPAEQQHQYL
                     PLQLIADFAFPHRLRR"
     gene            1151..1432
                     /locus_tag="PK348_10540"
     CDS             1151..1432
                     /locus_tag="PK348_10540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXK66697.1"
                     /translation="MKNMHDKKVGAFLVENGIISEEQLQEALELQRDNPERLIGEILV
                     TMGVLTKEELVMALEMYMMTTDAMPEHVDEWLDQDEIDLLMEKIKNESK"
BASE COUNT          484 a          254 c          311 g          466 t
ORIGIN      
        1 ttagcactca tgaaattgct acaaagtata tagccggaaa gcttccaaag aaaatgcact
       61 ggtattgtac aagagtgaag aaagtgatat tttcattcat gacatcatta aattacaccg
      121 tatagccaaa gttatctata ctctggaaga ggtgtatgct ctgctggtgc agcttatgtt
      181 tcgacaacaa ttacattcgc tgctgtattc attatatgaa acttcagaca tgcaacgcag
      241 tacaacagct tataaaggta gcttgcgtca gctgtatctt gaaactggta tggaaatttt
      301 tgcactgcct gccataacac atactgaaac aatagaatta ttcagggata acacagaacg
      361 tatgcatact atactggcaa aagcagcagg tttcagctgg cttagtgaca atgaataatt
      421 aacggcggag cctgtgggga aatgcaaagt cagcaatcag ttgtagggga aggtattgat
      481 gctgttgctc tgcagggtct ttggggaagc atggccgttt ttcattgtgg cagcttctgc
      541 atactttttc cctggctttt atgccatctg ttcccaggat tggatacatc ccaaatgaaa
      601 gggccttttt atatgcaact tccctgttgc ccagagccac atggttttca taactattgt
      661 acatactgcc tggaccatgg catgactcac atccaacacc atcttcccac agagcagggt
      721 ttttacctaa gcctgtagtg tgacatgcaa ggcacttttc attgtcggaa ggattttcaa
      781 tagataattc ttttgcaagc gataatgctt tttcctgttt aagcagtaaa tatgcttttg
      841 catgagggga ttgctgccag gatttatact gattaccaat agcctctgat tcatgacact
      901 ttttacagac agcaacacca acaaatgaag cgttttttcg tgctgccaaa aaggcacatg
      961 gtaacagtaa ccacactatg tagattctca ttgatttcat agcaaaagta acaatagcta
     1021 aagtatagcg ttatgaattg tttgtaaata cttttttata tgggcgatag ttcttaataa
     1081 ataatttttg gcttgactta ttaaattttt aatattaata ttaagtaaaa taatacgaag
     1141 cgaattatac atgaaaaata tgcatgataa aaaggttgga gctttcctgg ttgaaaatgg
     1201 tatcatttct gaggagcaat tgcaggaggc cctggaacta cagcgagata atcctgagag
     1261 gcttattggt gaaattcttg taaccatggg agttttaact aaagaagagc tggttatggc
     1321 tttagaaatg tatatgatga ctacagatgc aatgccggaa catgtagatg agtggcttga
     1381 tcaggatgaa attgatttat tgatggaaaa aataaagaat gaaagtaaat gattgaaaaa
     1441 gggtaaatca tctatttttg agtaaatgct caataacgta aaaagttctt tacaattatg
     1501 cattgatttt aaaaa
//
LOCUS       DAOSZP010000571         1508 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_61691_length_1508_cov_3.315898, whole genome shotgun sequence.
ACCESSION   DAOSZP010000571 DAOSZP010000000
VERSION     DAOSZP010000571.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1508
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61691_length_1508_cov_3.315898"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1508)
                     /locus_tag="PK348_10545"
     CDS             complement(<1..>1508)
                     /locus_tag="PK348_10545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014454223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase-related protein"
                     /protein_id="HXK66698.1"
                     /translation="VESAYCFPAEIAHGAVKNIFDKHVDYIFLPHSKEMKSYQNDARA
                     TFCPITQGLPYYIEKAFDEIPKEKILSPVISFKLSDTITEDSFVNVAVKLGFSSEEGK
                     RAFRLALQKQHEYFVKARKLGEEALEFARKLNKPAIALLGRPYNAFTKDANMGIPKKY
                     TSIGYTVIPFDILPFEDEEIFPNMYWFYGQLDMKASVMLAKENNIYVTFISNFSCAPD
                     SFMLHYLKWIMGSKPFLILELDSHTADAGIDTRIEAFLDIIEGYRQKCMGEETQRYDN
                     GLRFVIEDNELYVRNIVTNSKIPVRNNKKVTLLLASMGRLGAELLAASLRSAGINAEA
                     MPVPDAKTLQLARQFVSGKECLPSHLVLGSALQYFASPRYKKDEIYLLFVPTTQGPCR
                     TGQYFVFYENLFKDMRFDNVCVVTLDSSHSYTELGPHFSKHAWWGVVISDYMKDIETS
                     LRACAKDPVSAMALYDQLWQELISVAEKDVTRVIPVLKKIAQTISNIPLQRT"
BASE COUNT          438 a          320 c          273 g          477 t
ORIGIN      
        1 agttctctgt aatgggatat tgctgatagt ttgcgcaatt ttctttaaca cggggataac
       61 tctggtcaca tccttttcag ctactgagat taactcctgc cacaactggt catacagagc
      121 cattgcgctt acgggatcct tggcacatgc tctgagcgac gtttcgatgt ccttcatata
      181 atcactgatg acaactcccc accatgcatg ctttgagaag tgtggcccca gttctgtata
      241 ggaatgtgat gaatccaaag ttactacaca tacattatca aaacgcatgt ccttaaaaag
      301 attttcataa aaaacaaagt actgccctgt tcggcacggt ccctgtgttg ttggcacaaa
      361 taaaagatat atttcatctt ttttatatcg tggtgaagca aagtactgca atgcacttcc
      421 cagtaccaga tgtgaaggca ggcactcctt acctgacaca aattgtcgag ccagttgcaa
      481 tgttttagca tcaggcactg gcattgcctc agcattaatc ccagccgaac gaagtgacgc
      541 tgctaaaagt tctgcgccta aacgccccat gcttgctaaa agtaatgtaa cctttttatt
      601 attgcgaact ggtatcttac tattagtaac tatattgcgt acgtacaatt cattatcttc
      661 aatgacaaag cgcaggccat tatcatagcg ctgggtttct tcacccatac atttttgtct
      721 gtagccttca ataatatcca ggaaagcttc aatgcgtgta tctatcccgg catcagccgt
      781 atgtgagtca agctccagga taagaaatgg ctttgaaccc ataatccatt tcagatagtg
      841 taacatgaat gaatcaggtg cacatgaaaa gtttgaaata aatgttacat aaatattatt
      901 ttcttttgca agcatgacgc ttgccttcat atccagctga ccataaaacc agtacatgtt
      961 aggaaatatt tcctcatctt caaagggaag aatatcaaac ggaataaccg tatatccaat
     1021 actggtatat ttttttggta tgcccatgtt tgcatctttt gtgaatgcat tatatggtcg
     1081 ccccagtaat gctatggcag gtttattgag tttccgtgca aactctaacg cctcttcccc
     1141 caattttctg gcttttacaa aatattcatg ttgcttttgt aatgcaagcc tgaatgctct
     1201 tttcccttct tcagatgaaa atccaagttt tacagcaaca tttacaaatg aatcttcagt
     1261 aattgtatct gatagtttaa agctaataac tggtgaaagt attttttctt taggaatttc
     1321 atcaaatgcc ttttcaatat aatatggtaa cccttgagta atggggcaaa acgttgcacg
     1381 ggcatcattc tgatagctct tcatttcttt actgtgtggc aggaagatat aatcaacatg
     1441 tttatcaaaa atatttttaa cagcgccgtg agctatctca gcagggaagc aataggcact
     1501 ttcaacct
//
LOCUS       DAOSZP010000572         1503 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2056
            NODE_61984_length_1503_cov_3.112569, whole genome shotgun sequence.
ACCESSION   DAOSZP010000572 DAOSZP010000000
VERSION     DAOSZP010000572.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864123
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1503)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1503)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:54:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,110
            CDSs (total)                      :: 2,096
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 14
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1503
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61984_length_1503_cov_3.112569"
                     /isolate="AMR_MDS_2056"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1481)
                     /locus_tag="PK348_10550"
     CDS             complement(<1..1481)
                     /locus_tag="PK348_10550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HXK66699.1"
                     /translation="MSGGVLSDDLFFVYSNNAGKSFSNPRRITTSPANDSSPSILLHN
                     DVIYCVYQNNEKRQWDVRLLKGYNRGKEWEQQPVIVTDINVDCYNPRIVASSDDELLV
                     VWHDMRERYPAVFGRKVSLIDNSMTAEVRISDANVTSYDQFPVVIKKQVLLFWNSNQR
                     LMAKFSDISVLPPNVYSRTHPENVWTRNTNAIIEWDPPVDESGIAGYAVIYNDLPTFD
                     PTVQNVEGPIRKYVVPDLKDGITYFHIRAIDKAGNYSRTIHYPLLVSSTPLPMPTVSS
                     PTHPEATQVDNPVAVVQWTIEQRDRLKGFYYAIAKDAIVEPSTFTTADSVTLESLPKG
                     RYFFSIRPVDMTNMKGRMATYEIVIGDVGKADAAYYERLAKGVLPPEELPEKVPGKVR
                     IQYPIVLFNIPFKEIYASSFYKFSLLPKNILPKQVQGYSVVVSSQKVVPPFIVTHPTS
                     GVMLTNVKPGSYTITARMRYVYYQNGKAVYKWSAPSYTTLVVKS"
BASE COUNT          446 a          315 c          288 g          454 t
ORIGIN      
        1 gatttaacaa ccagcgtggt ataactgggt gcactccatt tatatactgc tttgccattc
       61 tggtaataca cataccgcat acgggcagtt atggtgtaac tgccaggttt tacattggta
      121 agcattaccc cactggttgg gtgggtaacg ataaaaggtg gtactacttt ttgtgaagaa
      181 acaacaactg aatacccctg aacctgcttt ggcaaaatat tttttggtaa taatgaaaat
      241 ttgtaaaatg aagacgcata aatttcttta aacggtatat taaaaagcac aatgggatac
      301 tgtattctta cctttcccgg gactttttct ggtaactctt ctggtggtaa aacacctttg
      361 gctaaacgtt cataatatgc cgcatcagct tttccaacat ccccaataac tatctcatat
      421 gttgccatcc gacctttcat gttggtcata tcaacaggac gaatgctgaa aaaatatctc
      481 ccttttggaa ggctttcaag agtcactgaa tcagcagttg tgaatgttga tggttcaaca
      541 attgcatcct tggcaatagc atagtaaaat cccttaagtc tgtccctttg ttcaattgtc
      601 cactgcacta ctgcaactgg attatcaacc tgtgtggctt caggatgtgt tggagagctc
      661 actgtgggca tgggtagtgg cgtactggaa accagcaacg gataatggat ggtgcggctg
      721 taattgccag ctttatcaat agcgcgaata tgaaaatagg ttataccatc ctttaaatct
      781 ggaacaacat attttcgtat tggcccttcc acgttctgta ctgttggatc aaatgtggga
      841 aggtcattat aaataacagc atatccagct atacctgatt catcaactgg cggatcccat
      901 tcaatgatgg catttgtgtt gcgggtccat acattttctg gatgtgtccg tgaataaacg
      961 tttggaggaa gtacagaaat atcagaaaac tttgccataa ggcgctggtt gctattccag
     1021 aacaacagaa cttgcttttt aataacaaca ggaaactgat catatgacgt tacatttgca
     1081 tcagatattc gcacttctgc agtcattgaa ttatcaataa gagatacttt cctaccaaat
     1141 actgcaggat agcgttcacg catatcatgc cacactacaa gcaattcatc atctgaagaa
     1201 gcaacgatgc gagggttgta acaatcaacg tttatatcag ttactataac cggttgctgc
     1261 tcccactcct ttcccctgtt ataccccttt aaaagccgta catcccattg cctcttttca
     1321 ttattctgat acacacagta aataacatca ttgtgcaaca gtattgaagg agatgaatca
     1381 ttagccgggc ttgtggtaat cctccgtgga tttgaaaaac tttttccggc attatttgaa
     1441 taaacaaaaa acaggtcatc agataatacc cctccggaca atatctttgc ctgaaatact
     1501 aaa
//
